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From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 1 Jan 2001 09:37:39 -0600
Subject: Administrivia: December 2000 Archives now on-line

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Colleagues...

The December 2000 Archives are now on-line at :

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

and welcome to the New Millenium...

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Jan 1 12:51:47 2001



From: simon watkins :      swatkins+-at-pitt.edu
Date: Tue, 02 Jan 2001 09:38:33 -0500
Subject: 3rd annual course in quantitative fluorescence microscopy

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The answer to this very much depends on what you are charging
for. To find an answer, you need to identify (a) the net costs you
wish to recover: e.g. capital costs (to be recovered over what
period?, at what rate of interest?), running costs, salaries of direct
(em technician) and indirect (clerical, admin.) support staff
including overheads, any charges for space occupancy and
services etc., less any subsidies (b) make a realistic estimate of
the maximum number of hours per year that a charge can be
recovered from. The answer is a/b. Simple, isn't it? Well, no. In
practise you will probably find that b is dependent on a/b since all
categories of users are sensitive to price, but you will have to
model this differently for different categories of users.

Our charges per hour for self-help access to a Philips CM120
Biotwin are as follows: Departmental £15, Other departments £21,
other publicly-funded research organisations £35, industrial £70.
Non-attenders incur full charge for the period booked. These
numbers were not exactly arrived at using rocket science. We
charge extra for all materials consumed (film, processing) and for
direct user-support from a technician. We do not include capital
costs recovery in our charges.

I would strongly advise imposing a special concessionary charge
on users who insist on tipping their specimens and retaining
circlips into the guts of the Compustage! An electronically-operated
trap door beneath the operator's seat is also an attractive option.



Chris




} From: "Ping Li" {pli-at-is.dal.ca}
To: {Microscopy-at-sparc5.microscopy.com}


Folks: A Happy New Year to you all
I thought I should let you all know about the Third annual course in
Quantitative Fluorescence Microscopy to be taught between june 17 and 22th
2001 at the Mount Desert Island Marine Biology Laboratories in Arcadia
National Park in Maine. This team taught course led by Dave Piston
(Vanderbilt), Mike Nathanson (Yale), Guillermo Romero (Pittsburgh) and
myself focusses specifically on the development and application of modern
fluorescence microscopic methods. This six day intensive course covers all
aspects of the technology from microscope and dye design, cameras, confocal
microscopy, live cell microscopy, multiphoton microscopy and GFP.
Considerable attention is also given to quantitative analysis in 2 and 3
dimensions and time. The specific focus of the course allows an in depth
treatment of these methods. The goal of the course it to teach students how
to best implement these methods within their labs, using either their own
cells and tissues or using material supplied by the course. An extensive
array instrumentation, provided by all the major microscope and associated
software, hardware and camera manufacturers will be available for students
to use.
For the last two years it has been a very successful event and so we have
decided to give the course again. A full description of the course lectures
together with lecture outlines, registration etc. is available at
http://sbic6.sbic.pitt.edu/microscopy/ I would encourage you to spread the
word, or sign up if you are interested. The total number of students is
limited to 25, enrollment is decided by the course faculty.
If you have any further questions please feel free to contact me
Thanks
Simon

---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------



From daemon Tue Jan 2 08:52:25 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 02 Jan 2001 09:41:03 -0500
Subject: Fwd: Position Open

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} There is a position for an EM technician at the Electron
} Microscopy Core lab of the Biotechnology Program at the University of
} Florida in Gainesville. The laboratory is mostly a fee-for-service lab
} serving the needs of biological, biomedical and agricultural scientists
} at the university. Applicants must be skilled in the preparation of
} biological samples for both scanning and transmission electron
} microscopy. Experience with both PC and Mac as well as website
} management is desirable. Experience with digital imaging and
} fluorescence microscopy also a plus.
} This is a full time, permanent position with standard benefits of
} State of Florida employees.
}
} For further details, reply to this message or contact Greg Erdos
} at the number below.
}
}
}

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251


From daemon Tue Jan 2 10:48:43 2001



From: Tracy Anderson :      tanderson-at-surmodics.com
Date: Tue, 2 Jan 2001 10:42:43 -0600
Subject: S-TPPS Protocol

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Does anyone have a standard protocol for using Silver Tetraphenylporphin
Sulfonate (S-TPPS) as an SEM stain for collagen?

Tracy E. Anderson
Surface Characterization Specialist
SurModics, Inc.
952.829.2720 Voice
952.829.2743 Fax
tanderson-at-surmodics.com



From daemon Tue Jan 2 12:46:50 2001



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 2 Jan 2001 18:25:54 -0000
Subject: Computing for microscopy and imaging

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As part of the planned makeover of our building, it has been
proposed that the various and somewhat distributed microscopy
facilities in our building - SEM, TEM, Confocal, fluorescence,
luminescence imaging, darkroom etc. etc. might be brought together
to form a biological imaging facility, thereby benefitting from some
improvements in supervision, security and user support. Part of that
proposal was to set up a computing lab with fileserver and work-
stations networked to the instruments and dedicated to image
storage and off-line image and spectral processing and analysis.
However, there is great pressure on space in this building, and it
has been suggested to me that computing for imaging and
spectroscopy does not need to be separated from general purpose
computing these days. What are your opinions about this? Pro or
con?

Happy New Millennium
Chris
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Tue Jan 2 13:13:22 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Tue, 2 Jan 2001 14:16:05 -0500
Subject: Re: Uranyl Acetate in Acetone

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Hi Tim,
I like your idea of a separate step for the UA*.
Though 1% may be overkill.
We routinely use a cocktail for freeze-sub consisting of:
1) 4% osmium tetroxide in HPLC grade acetone
2) 0.1% uranyl acetate also made with HPLC grade acetone - make this up the
day before the freezing so it has time to go into solution before chilling.
Mix together 1:1 for wonderful contrast.
*We have to use Radiation Safety Services for disposal of this cocktail
because our hazardous waste folks refuse to deal with the combination of
OsO4 and UA.

Another angle - You didn't mention the type of resin you're using.
Freeze-sub tissue is often murky looking (low contrast) when it is embedded
in 100% Spurr's. We use Araldite/Embed 812 (an EMS kit) or a mixture of
equal parts Spurr's and Araldite/Embed 812.

hope this helps,
Beth

} Hello Out There:
}
} I am currently involved with a cryo substitution project with human red
} blood cells. I have replaced the non crystalline ice with 2% Osmium in 100%
} acetone and have OK morphology, but the contrast is a bit low. I am
} thinking about trying to follow the Osmium step with uranyl acetate, before
} embedding in plastic. Does any one out there know if 1%uranly acetate will
} be OK in 100% acetone? Thanks, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Tue Jan 2 15:51:00 2001



From: Brownell, Patrick :      patrick.brownell-at-weyerhaeuser.com
Date: Tue, 2 Jan 2001 13:36:45 -0800
Subject: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
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Hi all!

I use Ralph type glass knives for plastic sectioning of biological samples.
I am experiencing problems in making a proper knife. I have a TAAB
histoknifemaker (also says Reichart-Jung). Is there anyone willing to offer
expertise in this area?

-Patrick Brownell


From daemon Tue Jan 2 20:49:42 2001



From: Edsworth-at-aol.com
Date: Tue, 2 Jan 2001 21:44:52 EST
Subject: Chiller

Contents Retrieved from Microscopy Listserver Archives
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Within the last couple of days (and, therefore, not in the recently posted
archives) someone posted a note about a used chiller that they had for sale.
I've lost the note. If still available, could you please repeat the note to
my email address: edsworth-at-aol.com Thanks.

Ed Holdsworth
General Mgr.
SEMTEC Laboratories, Inc.


From daemon Wed Jan 3 03:51:27 2001



From: Labar Janos :      labar-at-mfa.kfki.hu
Date: Wed, 3 Jan 2001 12:07:54 +0100
Subject: TEM- electron diffraction: New version of the ProcessDiffraction program

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Dear Colleagues,

I wish you successful and happy new year.

Those of you who need to process electron diffraction ring patterns
(recorded from thin polycrystalline or amorphous samples in a TEM) might be
interested in the FREE program (for IBM PC, Windows op. system) that can be
downloaded from my home page (below). The program produces XRD-like output
from digitized SAED ring-patterns and compares to markers showing the
positions and intensities of known phases.
The newest version (1.2.0) of this ProcessDiffraction program provides
easier operation and new functionality:

* Built-in hints: what to do next (and how). Can be switched off.

* File-name and path can be specified for output. Default paths are
suggested separately for input SAED, input Marker and output. Manually
selected paths can be stored as default (during exit).

* No limit on BMP file-size.

* The automatic peak-list can be manually extended using Cursor.

* Selection of individual points (in the SAED for reading-out of d-value and
establishing correlation between distribution and SAED) is aided with a
Cursor with increased contrast and magnification.


* Units of X-axis can be changed to the physically meaningful scattering
vector [1/A] (in contrast to pixels).

* Several net distributions can be compared

* Individual markers have different colors.

* Renormalized Gross intensity can be calculated by excluding pixels with
zero intensity (i.e. corresponding to beam-blocking pin) from the averaging
process.

* Renormalized Net intensity can be calculated. Here points with intensity
above the average (over a given circle) are only included in the averaging.
Average intensity of bright pixels within a discontinous ring are only
calculated this way, giving more realistic intensity of a diffraction line
for comparison purposes.


* Show menu is included to select which curves to show in the graph.

* Possibility to cancel is introduced at several operations.

* Automatic (fast) refinement of centre is included. In dubious cases manual
selection of peak and valley is asked for.

* Calculation is double precision (basis of later extension of the software
for input files with several bytes/pixel).

* Several error conditions are eliminated (crop, conflicting user requests,
..)

I hope you will like the modifications and find this program useful and easy
to use. Download FREE as before from the same site.

I would appreciate having your comments and/or suggestions. Best regards:



Dr. Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar




From daemon Wed Jan 3 09:13:17 2001



From: Mark Aindow :      m.aindow-at-uconn.edu
Date: Wed, 3 Jan 2001 10:07:03 -0500
Subject: Postdoc Position

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University of Connecticut
Institute for Materials Science

Postdoctoral Position in Electron Microscopy

The Institute for Materials Science (IMS) at UConn is an
interdisciplinary center with the threefold mission of fostering
education, research and outreach in all areas of the materials
sciences. The Institute has a vacancy in the EM laboratory
for postdoctoral researcher to work on a DARPA-funded
program on Ni-based superalloys and a variety of smaller
industrial projects. Candidates should hold a PhD in Materials
Science, Physics or a related discipline and must have extensive
hands-on experience in a broad range of electron microscopy
techniques. A candidate with experience in the microstructural
analysis of Ni-based superalloys using SEM and TEM would
be preferred. The appointment is for one year in the first instance
and is available immediately. Screening of the applications will
begin immediately and will continue until the post is filled.
Applications from under-represented groups, including minorities,
women and people with disabilities are encouraged.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to:
Prof. M. Aindow, Institute for Materials Science, University of
Connecticut, 97 North Eagleville Road, U-3136, Storrs,
CT 06269-3136 USA. Email: m.aindow-at-uconn.edu


From daemon Wed Jan 3 09:29:13 2001



From: Dr. Catherine Powell :      POWCA-at-reg2.health.nb.ca
Date: Wed, 03 Jan 2001 11:24:38 -0400
Subject: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
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We are a clinical diagnostic TEM laboratory serving a provincial network of hospitals. We would like to move away from conventional darkroom printing of images and wish to convert negatives to digital files. We are looking for a negative scanner that will:

1. Accept 3 X 4 1/4 inch sheet film negatives (Kodak 4489 film).

2. Comes with software support that is adaptable to a Windows format.

3. Must be priced under $3000.

4. Must provide the best quality resolution for diagnostic results (i.e. 3200 X 3600 pixel; wide linear dynamic range).

I look forward to any information and advice you can provide.

Catherine Powell







From daemon Wed Jan 3 10:10:33 2001



From: Brownell, Patrick :      patrick.brownell-at-weyerhaeuser.com
Date: Wed, 3 Jan 2001 08:04:10 -0800
Subject: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
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My problem is a poor knife edge. The knife looks fine under casual
observation, or with small magnification, but when it comes time to actually
do some sectioning, I get a lot of chattering, or streaking, or both. I
know make as many test blocks as I do sample blocks, so I don't waste
samples with all the bad glass knifes. I've gone through several boxes of
glass over the past two months, and only get a useable knife every 15 to 20
breaks. The same knife breaker has worked fine in the past, and I have
changed the scoring wheel, and checked all that I can.

This knife breaker is also very hard to get parts for. The only obvious
problem is that one of the pressure points has a rubber pad that is unevenly
worn. I don't see how that would cause my problem. Again, the knifes look
good to the naked eye.

The glass I use is from Electron Microscopy Sciences. They are called ultra
glass knife strips 6.5mm x 25mm x 400 mm.

I guess I'm just looking for someone who has had a similar problem, and
fixed it in some manner. Could I have gotten a bad batch of glass? I don't
think it is very likely, I've used several boxes, but they may all be from
the same batch, for all I know. Is there another brand of glass, or breaker
that others have found produce reliable knives?

Thanks for any and all help

-Patrick Brownell
} ----------
} From: Katja M. Wolski[SMTP:kwolski-at-hsc.usf.edu]
} Reply To: kwolski-at-hsc.usf.edu
} Sent: Wednesday, January 03, 2001 4:26 AM
} To: Brownell, Patrick
} Subject: Re: Ralph Glass Knife problems
}
} I use a different knife machine, but what's the problem you're having?
} Are you
} not getting a good knife edge or what?
}
} Katja
}
}
}
} "Brownell, Patrick" wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hi all!
} }
} } I use Ralph type glass knives for plastic sectioning of biological
} samples.
} } I am experiencing problems in making a proper knife. I have a TAAB
} } histoknifemaker (also says Reichart-Jung). Is there anyone willing to
} offer
} } expertise in this area?
} }
} } -Patrick Brownell
}


From daemon Wed Jan 3 10:54:47 2001



From: John Foust :      jfoust-at-threedee.com
Date: Wed, 03 Jan 2001 10:49:57 -0600
Subject: Zeiss lamp failure question

Contents Retrieved from Microscopy Listserver Archives
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I recently acquired a Zeiss OPMI with floor stand.
I inspected the bulb superficially before turning
it on and it seemed OK, and I confirmed the voltage
was in range beforehand. However, as soon as I
applied power, the filament left the prongs and
the bulb was dead.

It was a Zeiss 390158 bulb, a 6v 30w triangular
bayonet. It's quite possible the bulb was
mechanically shocked before I got it. Was this
a not unusual failure, or is there some kind of
warm-up procedure that might've saved it?

A hunt through my junk boxes fortunately turned up
a replacement, and it worked straight-away, even though
it looks like one of the spring-like wires worked its
way off the filament prongs, where the filament meets
the prong wire.

For the day that this one dies, though, I'd also like
recommendations for a place to purchase a replacement.

- John



From daemon Wed Jan 3 15:01:29 2001



From: Tracy Anderson :      tanderson-at-surmodics.com
Date: Wed, 3 Jan 2001 14:44:41 -0600
Subject: Suggestions for a good sputtercoater?

Contents Retrieved from Microscopy Listserver Archives
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Greetings. Can anyone suggest a good all around sputter coater for general
SEM use? I usually use 50 Angstrom Pt, or Carbon for EDS. Thanks in
advance.

Tracy E. Anderson
Microscopy Specialist
Surface Characterization Dept.
SurModics, Inc.
www.surmodics.com
tanderson-at-surmodics.com



From daemon Wed Jan 3 15:38:45 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 03 Jan 2001 14:25:03 -0600
Subject: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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Anyone else happen to watch "Mysterious Ways" earlier this week. One
character was chatting with another about the fantastic new TEM they were
using with the "Kevex EDS system with the twin Quantum 10-mm2 detectors". I
had to listen quick to hear it.

Granted, its a bit dated (Thermo-Noran is now the name and they don't list
the Quantum on their web page), and some of their other references to other
techniques were of questionable accuracy. Still, I was tickled to see a
reference to EM on TV. I remember a few references on "Quincy", but not a
lot since.

Disclaimer - I don't have a twin Quantum system, but am still using a
10-mm2 Quantum on our JEOL-840.

Warren S.



From daemon Wed Jan 3 15:58:23 2001



From: greg :      greg-at-umic.sunysb.edu
Date: Wed, 03 Jan 2001 16:57:32 -0500
Subject: ultramicrotomes

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Hi all:
Can anyone tell me who the current manufactures
of ultra microtomes are?
--
Regards,
Gregory Rudomen
Technical Specialist Electron Microscopy
State University of New York at Stony Brook
University Microscopy Imaging Center
Stony Brook, NY 11794-8088
631-444-7372 Greg-at-umic.sunysb.edu
*************************************************
Standard disclaimer: The opinions expressed
in this communication are my own and do
not necessarily reflect those of the University
Microscopy Imaging Center.
*************************************************


From daemon Wed Jan 3 17:18:45 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Wed, 3 Jan 2001 17:12:38 -0600
Subject: RE: Zeiss lamp failure question

Contents Retrieved from Microscopy Listserver Archives
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John:

Bulbs of the most exotic sort can be found at:
1. Bulbtronics;(516)-249-2272
2. Bulb Direct; 1-800-772-5267, or info-at-bulbdrect.com

Good luck,

Sam Purdy
Technical Center,
National Steel Corp.
Trenton MI 48183
Voice; 734-676-2682
FAX 734-676-2030
E-mail spurdy-at-nationalsteel.com
} ----------
} From: John Foust
} Sent: Wednesday, January 2001, 11:49 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Zeiss lamp failure question
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I recently acquired a Zeiss OPMI with floor stand.
} I inspected the bulb superficially before turning
} it on and it seemed OK, and I confirmed the voltage
} was in range beforehand. However, as soon as I
} applied power, the filament left the prongs and
} the bulb was dead.
}
} It was a Zeiss 390158 bulb, a 6v 30w triangular
} bayonet. It's quite possible the bulb was
} mechanically shocked before I got it. Was this
} a not unusual failure, or is there some kind of
} warm-up procedure that might've saved it?
}
} A hunt through my junk boxes fortunately turned up
} a replacement, and it worked straight-away, even though
} it looks like one of the spring-like wires worked its
} way off the filament prongs, where the filament meets
} the prong wire.
}
} For the day that this one dies, though, I'd also like
} recommendations for a place to purchase a replacement.
}
} - John
}
}




From daemon Wed Jan 3 18:18:39 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 03 Jan 2001 19:20:03 -0500
Subject: "good all around sputter coater"

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tracey E. Anderson wrote:
======================================================
Greetings. Can anyone suggest a good all around sputter coater for general
SEM use? I usually use 50 Angstrom Pt, or Carbon for EDS. Thanks in
advance.
======================================================
SPI Supplies manufactures a modular coater that is robust, requires little
maintenance, and will do all of the precious group metals, including Pt.
Carbon is done via the carbon coater module. A number of other firms
manufacture sputter coaters for SEM applications and they can all be found
on the following two vendor data bases:

Microscopy Vendor's Data Base
http://207.137.96.185/mvd/vendors.html

MicroWorld Resources and News
http://www.mwrn.com/

Disclaimer: SPI Supplies manufactures sputter and carbon coaters for SEM
laboratory applications.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================









From daemon Wed Jan 3 18:20:17 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Wed, 3 Jan 2001 18:03:42 -0600
Subject: MAS Membership Renewal Applications

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MAS membership renewal application forms were mailed late last week.
Enclosed with your application is the 2000 Membership Directory. Please
make any corrections on your form and return it along with your dues in the
envelope provided. My sincere apology for the late mailing.

If you have any questions, do not receive your renewal packet, or are not a
member of MAS and would like to join, please contact me either at work or
at:

MAS Membership Services
PMB 141
2101 W. Broadway
Columbia, MO 65203-1261
1 (800) 4MASMEM
masmembership-at-excite.com

Thanks,
Lou Ross
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.missouri.edu/~geosclmr/ebaf.html


From daemon Wed Jan 3 21:35:34 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, January 03, 2001 1:31 PM
Subject: Zeiss lamp failure question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John, check the following places.

http://www.sylvania.com/ http://topbulb.com/
http://www.osram.com/ http://www.spectraservices.com/
http://opto.perkinelmer.com/products/catalog/categories/cat3.asp
http://www1.ushio.co.jp/ http://www.arc-lamps.com/

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: John Foust {jfoust-at-threedee.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Wed Jan 3 22:02:50 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 03 Jan 2001 19:58:16 -0800
Subject: Re: Suggestions for a good sputtercoater?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What constitutes a "good all around" sputter coater is perhaps
somewhat vague? If you are looking for something that is
easy to use and provides consistent results, that may be
a problem.

New units are in the $4K to $8K price range. This depends
on whether it has a pump included, thickness measuring
unit (not all that usefull, IMO) and type of target, and dual
mode or single plating mode. These units are sputter
coaters, not evaporation units.

I searched for what it sounds like you are doing the same.
I narrowed the search to a Denton Desk II or an Anatech
Hummer VII. I settled on a used Hummer. This is an obsoleted
unit. But after some initial hickups, this coater works really
well. Everything is contained in one unit. It has old metal
gate CMOS digital ICs (still available) and an Edwards or
Alcatel small pump inside. It will do etching and plating.
What I like about this unit is that it will do plating at a specified
rate with a specified end point thickness. These settings
must be validated to be absolutely correct. The rate and
power can be adjusted via pots to hone the correlation of
settings and results.

Newer units cost tons of money and do not do a proportionately
better job. They are basically manual units. Set the time, and
make the run. No idea of thickness. Pump is optional...right.
Thickness measurement options are a waste of money in
my opinion. The results are not repeatable. And the most
common measuring method is a quartz crystal. This is a
consumable item.

You have a tough decision, based on my experience. I would
suggest that you look for a used Hummer VII or a Desk II.
I just don't think that the newer units are all that great of a value.
Nor are they all that user friendly. But YMMV.

gary g.


At 12:44 PM 1/3/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jan 3 23:01:39 2001



From: integer-at-www.god-emil.dk
Date: Thu, 4 Jan 2001 05:57:31 +0100
Subject: Microscope recommendation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hallo,

I am participating in an art project that incorporates micro-organisms
(e.g. euglena although larger ones e.g. hydra are feasible) as actors.
The video from the microscopes/cameras will be streamed in realtime via the Internet.

Image quality is important to us, cost less so although there shall
be several stations in different geographical locations.

Should one wish to suggest a particular microscope and camera we would be delighted.
(The software isn't an issue)

Gelukkig Nieuw Jaar
Amicalement, NN





From daemon Thu Jan 4 03:02:41 2001



From: Cameron Hind :      Cameron_Hind-at-baxter.com
Date: Thu, 4 Jan 2001 09:50:39 -0600
Subject: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi Listservers and Catherine Powell,

A European company -Digital Biomedical Imaging Systems AG (DITABIS),
produce a plate scanning system that may suit your future needs, but
I'm not certain whether you can scan negatives with it. It's an
imaging system to replace using negatives; I don't know if they have a
distributor State-side but a while back I made contact with a British
company at the Micro 2000 conference, UK, who are distributors for the
system in the UK and they were willing to let customers have a trial
run with the scanning plates.

A web site http://www.ditabis.de/first.html explains the system

The UK company is

ISS group services
Pellowe House
Francis Rd
Withington
Manchester M20 9XP

Phone:+44 161 445 5442
Fax:+4 161 445 4914

They don't appear to have a web site

Hope this info is of some use + I stress that I have absolutely no
commercial or other interests with the above companies.


Mr. Cameron Hind
Research Scientist
Advanced Technologies group
Baxter R & D Europe S.C.R.L.
Rue du Progrès 7
1400 Nivelles
Belgium

Tel:+32 67 882 511
Fax:+32 67 217 191
e-mail: hindc-at-baxter.com





















From daemon Thu Jan 4 07:24:25 2001



From: Robert_Fowler%TDK-US-at-TDKTCA.COM
Date: Thu, 4 Jan 2001 08:18:59 -0500
Subject: Re: Zeiss lamp failure question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi John
In reference to your request for replacement bulb 39-01-58...I currently
use Bulb Direct for all my lighting needs. 1-800-772-5267
WWW.BULBDIRECT.COM This company is very good on delivery (2 days) and have
the best prices I have run across.



I have no affiliation with Bulb Direct just satisfied customer.

Robert



From daemon Thu Jan 4 07:44:56 2001



From: jo verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Thu, 04 Jan 2001 14:32:58 +0000
Subject: Chemical reference sample for EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,


Can anyone advise me on what chemical sample I can use to test the
(in)accuracy of EELS quantification for oxygen. Is there an oxide for
which there is abselutely no doubt on the oxygen concentration.
Preferably with a metal which has a good EELS peak (below 1 keV). The
goal is to have a reference with a reliable metal/O ratio.

Thanks,

Jo



From daemon Thu Jan 4 08:13:40 2001



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Thu, 4 Jan 2001 08:06:52 -0600
Subject: job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Facility Manager Position
Electron Probe Instrumentation Center
Northwestern University

The Electron Probe Instrumentation Center (EPIC) at Northwestern University
has an immediate opening for a full-time facility manager to assume the
responsibility of managing the advanced EM laboratory and
facilitate/initiate advanced TEM research. Responsibilities also include
supervision of two technical staff in SEM and specimen preparation.
The EPIC facility serves over 100 users in all aspects of Scanning and
Transmission electron microscopy. The role of the Facility Manager is to
provide leadership in advanced electron microscopy in materials research,
and assist users with their advanced TEM needs, including the following:
teaching/assistance in: HRTEM, nanodiffraction, EDS, EELS, image processing,
etc. A strong laboratory development component, research initiatives and
commitment to teaching are associated with this position.
All EMs in EPIC are under full service contract. Thus, the duties include
mainly training students/users in their research needs, development of
specialized techniques and applications for materials research, and minor
records and fiscal management.
A PhD in physical sciences/engineering is required. The candidate must have
hands-on experience in all aspects of advanced TEM as well as digital
acquisition, processing and computer assisted techniques. All levels of
experience will be considered. Compensation commensurate with experience and
qualifications. Promotion to research faculty position is possible with
proven research credentials.
Send cover letter, resume and three references to:
Prof. Vinayak P. Dravid, Director EPIC
Materials Science & Engineering
Northwestern University, 3013A MLSB
Evanston, IL 60208
E-mail: v-dravid-at-northwestern.edu
Fax: (847) 491-7820
http://epic.ms.nwu.edu/epic/index.htm

Northwestern University is an Affirmative Action/Equal Opportunity Employer.
Hiring is contingent upon eligibility to work in the United States.






From daemon Thu Jan 4 08:35:30 2001



From: Cavin Mooers :      cavinm-at-vsl.cua.edu
Date: Thu, 4 Jan 2001 08:30:23 -0600
Subject: Ultramicrotomy of Glass Subjected to Vapor Hydration Testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I am a research assistant at Catholic University and have been
attempting for the past few months to thin section alteration layers,
(in cross section,) of simulated nuclear waste glasses subjected to
vapor hydration testing. These altered layers are typically comprised
of multiple phases, frequently composed of various alumino-silicates,
and are brittle and porous. They range from a few microns to hundreds
of microns. When fragmented off the test coupon, usually a few microns
(~1-20um) of glass remain adhered to altered region. We are interested
in this transition region between the glass and altered layers.

Dozens of attempts have been made to section these materials once
embedded. Variations of impregnation protocols have been tried with
recipe variations utilizing Spurr’s resin. Block faces on average have
been ~200x500um with particles sizes anywhere from a few microns to
~200um. Utilizing a Sorvel MT-2, cutting speeds between 0.25 and
2.5mm/second have been attempted, (the full range of the instrument.)
The diamond knife is new. The advance has been altered between 20nm and
250nm – producing the appropriate interference colors, but with voided
regions wherever the particles are situated. I have tried everything I
could think of to
facilitate infiltration/adhesion of these particles within the embedment
medium. After thousands of sections from 80+ blocks and several
protocols, I haven’t had the slightest glimmer of success. I know the
very thing we have been attempting has been successfully done at ANL.
So, I implore anybody that has any specific insight, simple thoughts, or
longshots to let me know.

Sincerely,

Cavin T. F. Mooers
Research Assistant
The Catholic University of America
434 Hannan Hall
Washington, D.C. 20064
(202) 319-5346 phn
(202) 319-4469 fax




From daemon Thu Jan 4 09:55:24 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 4 Jan 2001 11:48:03 -0400
Subject: Leitz TAS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers-

And now, from the Last Hope Department, I was wondering if anyone out
there has an old Leitz Tas Plus Image Analyzer? This would be of mid-'80's
vintage, comprised of a bench with a pop-up monitor with light pen, a nice
microscope with built-in video capability, and a big black electronics box
about four feet tall (containing 2 8-inch floppy drives - like I said, this
thing is old).
Long story short, ours is broken, and we can't even begin to fix it
without schematics (some clever soul -no, not me - tossed them out years
ago).
If anyone happens to have a set of the electronics schematics for one of
these things hanging around, please contact me ....we'll talk....

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
P.O. Box 1006
Dartmouth, Nova Scotia
Canada
B2Y 4A2



From daemon Thu Jan 4 12:16:44 2001



From: C Daniels :      cdaniels-at-gatan.com
Date: Thu, 4 Jan 2001 10:10:47 -0800
Subject: TEM job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TEM Applications Laboratory Manager - Gatan, Inc.

Gatan, Inc., the technology leader in digital imaging, analytical
spectroscopy and icon beam milling applications for electron microscopy in
Pleasanton, CA., is seeking candidates for a TEM Laboratory Manager. This
person will manage the TEM applications laboratory. Responsibilities
include coordinating all activities related to customer demonstrations and
training, Applications material generation and supporting the R&D department
utilization of the TEM systems. Some travel within and outside the USA is
required. Applicants should have significant TEM and CCD imaging
experience. Biological, materials or semiconductor experience would be
useful. Familiarity with existing vendors, consultants, competitors, etc in
the industry is a plus. Knowledge of Gatan's software and hardware
applications. A proven track record in both planning and managing a
laboratory environment and outstanding computer/PC skills. Must be
comfortable with current development environments and development tools to
work intelligently with engineering. Must have excellent overall PC
computing skills, including a thorough familiarity with market tools in
document composition, database design and presentation management. PhD,
desired; technical background preferred. Salary: Base salary plus bonus
commensurate with experience.

Interested candidates should send, email or fax their resume to:

GATAN, INC
Attn: HR Department
5933 Coronado Lane
Pleasanton, CA. 94588
Fax: (925) 463-0204
Email: hr-at-gatan.com
www.gatan.com
Carlotta Daniels
GATAN, Inc.
Human Resources




From daemon Thu Jan 4 13:46:52 2001



From: phil.swab-at-depsci.com (Phil Swab)
Date: Thu, 4 Jan 2001 11:48:14 -0800
Subject: Ultramicrotomy of Glass Subjected to Vapor Hydration Testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Calvin,
Hard materials such as glass can be readily microtomed with a diamond knife
(even diamond coated silicon in Microscopy Research and Technique, vol. 31,
p. 308, 1995). Any good diamond knife will work with meticulous and careful
technique, but experience has shown that 35 degree knives yield the best
results with hard and ultra-hard materials.

It sounds as though your samples may be large and adhesion to the epoxy is
poor. In order to microtome non-porous materials such as glass, you should
first minimize the cross-sectional area to be sectioned. An easy way is to
do this is to pop concoidal micro-chips from the surface. These tend to be
very thin at the edges and can be further broken to form pointed thin
samples. Another critical element is to improve adhesion of the sample to
the resin (Spurrs) with the use of an adhesion promoter such as Dow Corning
Z-6040. Submerging the sample in a 2% solution of Z-6040 (50/50 ethanol
and H2O) for 30 minutes allows a surface reaction that promotes adhesion of
glass to epoxy. Remove samples from the solution, air dry, and embed in
Spurrs. Then, microtome using standard procedures with sectioning speed
reduced to 0.1 mm/s.

Cheers,


Phil Swab, Sr. Engineer
Engineering Development Group
Deposition Sciences, Inc
386 Tesconi Court
Santa Rosa, CA 95401

phil.swab-at-depsci.com
707-566-3718 (Phone)
707-573-6755 (FAX)

-----Original Message-----
} From: Cavin Mooers [SMTP:cavinm-at-vsl.cua.edu]
Sent: Thursday, January 04, 2001 6:30 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Microscopists,

I am a research assistant at Catholic University and have been
attempting for the past few months to thin section alteration layers,
(in cross section,) of simulated nuclear waste glasses subjected to
vapor hydration testing. These altered layers are typically comprised
of multiple phases, frequently composed of various alumino-silicates,
and are brittle and porous. They range from a few microns to hundreds
of microns. When fragmented off the test coupon, usually a few microns
(~1-20um) of glass remain adhered to altered region. We are interested
in this transition region between the glass and altered layers.

Dozens of attempts have been made to section these materials once
embedded. Variations of impregnation protocols have been tried with
recipe variations utilizing Spurr's resin. Block faces on average have
been ~200x500um with particles sizes anywhere from a few microns to
~200um. Utilizing a Sorvel MT-2, cutting speeds between 0.25 and
2.5mm/second have been attempted, (the full range of the instrument.)
The diamond knife is new. The advance has been altered between 20nm and
250nm - producing the appropriate interference colors, but with voided
regions wherever the particles are situated. I have tried everything I
could think of to
facilitate infiltration/adhesion of these particles within the embedment
medium. After thousands of sections from 80+ blocks and several
protocols, I haven't had the slightest glimmer of success. I know the
very thing we have been attempting has been successfully done at ANL.
So, I implore anybody that has any specific insight, simple thoughts, or
longshots to let me know.

Sincerely,

Cavin T. F. Mooers
Research Assistant
The Catholic University of America
434 Hannan Hall
Washington, D.C. 20064
(202) 319-5346 phn
(202) 319-4469 fax




From daemon Thu Jan 4 14:19:04 2001



From: Ping Li :      pli-at-is.dal.ca
Date: Thu, 04 Jan 2001 16:14:44 -0400
Subject: Help: Confocal micorscope imaging problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, we have some imaging problems with Zeiss LSM 410. Sometimes, when we
collect an image series, one or more of the images had different sized
lighter or darker bands across the images. This happened with both green
and red channels (488 nm and 543 nm). It occurred randomly. In addition,
sometimes, the image suddenly totally whited out during the scanning. In
which case, the up part of the image looks normal, but the lower part is
white. In all cases, there were no indication of any changes in pin hole
size, contrast, brightness, and laser power based on the readings showed
in the LSM program. I am not sure what is wrong. Could be the scanner?
video card? or may be something else? Could any of you please help me
and tell me what do you think the problems are? Any information is
highly appreciated.

Ping



From daemon Thu Jan 4 14:48:26 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 04 Jan 2001 12:47:13 -0800
Subject: Re: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Catherine

I am using regular EPSON Expression 1600 flatbed scanner with "transparency
adapter". It has 1600 optical dpi resolution and pretty fast. To
eliminate the Newton rings, you may use special cassettes to hold the
film. Unfortunately, EPSON does not provide the cassettes for standard EM
film. You have to made them in machine shop (not big deal, actually). I
do use some EPSON cassette, which is bigger than my film in one dimension,
but it works well. This scanner comes with very good software and you may
scan your images directly into Photoshop or any other suitable program. I
am scanning in "positive transparency", 12-bit (Hi-Fi) gray, 1600 dpi mode
and than adjust the levels in the Photoshop (to remove the "empty" levels
of gray), transfer in 8-bit mode and save. Usually, I prefer to keep the
original 12-bit image as well, but it's huge - about 50 Mb each. I am
using 2.6 or 5.2 Gb MO disks for storage.

I hope it will help.

Sergey

P.S. I have no interest in EPSON products, just happy user.

At 11:24 AM 1/3/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jan 4 14:57:04 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 04 Jan 2001 12:56:57 -0800
Subject: Re: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is another story, Cameron

This equipment specially designed to work with "image plates". Nothing
related to the negatives. Moreover, they claimed that their system is
supposed to substitute the regular photo-process (and in some instances
that works better, linearity and sensitivity, for instance). Only one
disadvantage - very pricey and time consuming.

Sergey.

At 09:50 AM 1/4/01 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jan 4 15:10:16 2001



From: weiliang EARTH.WGONG.PLANET. gong :      wgong-at-UNM.EDU
Date: Thu, 4 Jan 2001 14:07:13 -0700 (MST)
Subject: Re: Ultramicrotomy of Glass Subjected to Vapor Hydration Testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cavin,

I think Phil is right. You better collect really a small smaple which is
like a needdle. At the frond end of your needle, you will have the altered
layer plus a little glass. Crack your corroded glass samples you can
always find out a tiny needle suitable for ultramicrotomy. You may read
our paper in Journal of Nuclear Materials (Vol. 254, pp. 249-265, 1998)
for your reference.

Good luck,

Weiliang Gong
Center for Radioactive Waste Management
University of New Mexico
1001 University Blvd. Se
Albuquerque, NM 87106



From daemon Thu Jan 4 15:10:39 2001



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Thu, 4 Jan 2001 16:07:51 -0500
Subject: SEM scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello-
Does anyone out there know the scintillator sizes for the upper and
lower secondary electron detectors on a Hitachi S-4500? Also, does anyone
have an opinion on whether the single crystal YAP (or YAG) scintillators
are worth 10x the money of a P-47? The catalogues say they last longer and
have better S/N even though they produce less signal than the P-47. If I
switched, would there be adjustments I would need to make to my SEM?
Thanks for your help.

Sincerely,
Matthew Ervin



From daemon Thu Jan 4 15:34:59 2001



From: Demaree, Richard :      RDEMAREE-at-csuchico.edu
Date: Thu, 4 Jan 2001 13:31:26 -0800
Subject: Nikon coolpix at low light levels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

We are considering purchasing a Nikon Coolpix digital camera for use on
light microscopes. We would like to know if anyone has experience using
these cameras for fluorescent microscopy. Is the meter accurate at low
light levels? Thanks.

Richard Demaree, Ph.D.
Dept. Biological Sciences
Calif. State Univ., Chico
Chico, CA 95929-0515
530-898-5812
rdemaree-at-csuchico.edu



From daemon Thu Jan 4 15:36:34 2001



From: Kristi Snell :      snell-at-metabolix.com
Date: Thu, 04 Jan 2001 16:30:12 -0500
Subject: LM - immunofluorescence in plant protoplasts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to find a way to perform immunofluorescence
in plant protoplasts for both cytosolic and peroxisomal
proteins.

My problems are as follows:

1) Fixing of the protoplasts prior to immunofluorescence
is difficult;
fixation in 4% paraformaldehyde, 350 mM mannitol, and
50 mM sodium phosphate, pH 7 for 50 min followed
by treatment with 0.1% triton X-100 for 10 minutes
yields protoplasts that appear to be aggragated and
broken.

2) Fixing of protoplasts expressing cytosolic GFP,
as detailed above, loose their GFP-fluorescence.

Does anyone have an established method for plant protoplast
immunofluorescence?

Thanks

Kristi Snell

--
Dr. Kristi D. Snell
Metabolix, Inc.
Cambridge, MA






From daemon Thu Jan 4 15:44:03 2001



From: Tracy Anderson :      tanderson-at-surmodics.com
Date: Thu, 4 Jan 2001 15:29:22 -0600
Subject: TEM sample prep for glass slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings. I would like to look at a cross section of a standard sized
glass slide under TEM. Does anyone know how I could prepare such a sample?
Will a microtome work? Any suggestions would be very much appreciated.

Tracy E. Anderson
Surface Characterization Specialist
SurModics, Inc.
952.829.2720 Voice
952.829.2743 Fax
tanderson-at-surmodics.com



From daemon Thu Jan 4 16:34:07 2001



From: Kristi Snell :      snell-at-metabolix.com
Date: Thu, 04 Jan 2001 17:26:15 -0500
Subject: Re: LM - peroxisomal staining in plant protoplasts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} I am trying to find a way to stain peroxisomes
} in plant protoplasts and visualize the peroxisomes by light
} microscopy.

} Does anyone have an established method for this that doesn't break the
} fragile protoplasts?

} Thanks.
}
} Kristi Snell
}
} --
} Dr. Kristi D. Snell
} Metabolix, Inc.
} Cambridge, MA





From daemon Thu Jan 4 18:51:55 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 4 Jan 2001 19:47:09 -0500
Subject: RE: TEM sample prep for glass slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are a number of standard methods that will work.

Microtomy should work. There has been a recent thread about some porous glass. You should see the postings by Phil Swab. He also has a Journal article that you might be able to get a copy from Stacie Kirsch on how to do cross section of glass using microtomy. He recommends a 35 degree knife. Basically, you create small shards from the surface of the glass, mount them and then cut them. If you know how to microtme and do not know any of the other standard cross sectional preparation techniques, I would go with this one.

You could use the small angle cleavage technique. It is relatively inexpensive and easy to learn. It also works well with glass.

You can use the standard cross sectional method of Bravman and Sinclair. You can take two pieces of your glass slides about 5-10 mm x 10-20 mm and epoxy them together. I use Epo-Tek 353ND, but you can also use N-bond 610. I prefer to use a stack of silicon on one side for a number of reasons: 1) I believe that the Si helps reduce the charging effects, 2) I believe that the Si helps prevent the glass from heating under the beam, 3) I can use the Si to gauge the thickness of the sample during dimple grinding, and 4) the Si can help me find the orientation of the sample relative to the beam that puts the surface parallel to the beam. Cut the samples with a thickness of about 400-500 um with a diamond wheel cutoff saw, hand lap them with a hand grinding tool with parallel sides to between 60-90 um thick, dimple grind them to about 5 um (the Si will show a rich red color in transmitted light), and ion mill to perforation and electron transparency.

You can use Tripod polishing with a low angle ion mill clean-up. This takes some learning to do properly and specialized tools.

You can use an FIB. Glass cuts reasonable well without too much charging affects. Protect the surface with a relatively thick sputter coat of gold or Au-Pd before you put it in the FIB, especially if you use a single beam FIB.

With your finished sample, I found that at 125 kV I had to put a light carbon coat on the sample to prevent softening of the glass under the beam. AT higher voltages (200 kV or better), you don't need to coat the sample for charging or for eliminating the heating effects.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Tracy Anderson [mailto:tanderson-at-surmodics.com]
Sent: Thursday, January 04, 2001 4:29 PM
To: 'Microscopy ListServer'
Subject: TEM sample prep for glass slide


---------------------------------------------------------------
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of America
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Greetings. I would like to look at a cross section of a standard sized
glass slide under TEM. Does anyone know how I could prepare
such a sample?
Will a microtome work? Any suggestions would be very much appreciated.

Tracy E. Anderson
Surface Characterization Specialist
SurModics, Inc.
952.829.2720 Voice
952.829.2743 Fax
tanderson-at-surmodics.com




From daemon Thu Jan 4 19:39:02 2001



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Fri, 5 Jan 2001 10:35:54 +0900
Subject: Re: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: "Sergey Ryazantsev" {sryazant-at-ucla.edu}

} This equipment specially designed to work with "image plates". Nothing
} related to the negatives. Moreover, they claimed that their system is
} supposed to substitute the regular photo-process (and in some instances
} that works better, linearity and sensitivity, for instance). Only one
} disadvantage - very pricey and time consuming.

Yes, they are better for diffraction work (high dynamic range, linearity).
But why time consuming? You just put the plates in the scanner and go do
some other things. When you are back you have all the images scanned. No
development and stuff like with films.

Best regards,

Rado


---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------



From daemon Thu Jan 4 19:41:36 2001



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Fri, 5 Jan 2001 10:39:34 +0900
Subject: Re: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Sergey,

} I am using regular EPSON Expression 1600 flatbed scanner with
"transparency
} adapter". It has 1600 optical dpi resolution and pretty fast.

Have you tested the MTF of the EPSON? I'm interested what are the actual
resolutions X,Y.

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------



From daemon Thu Jan 4 21:04:47 2001



From: Winton Cornell :      winton-cornell-at-utulsa.edu
Date: Thu, 4 Jan 2001 20:58:48 -0600
Subject: problems with a Phaser 780 color laser-printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

We have a Tektronix Phaser 780 color laser-printer which is currently
out of service. We can not find anyone in Oklahoma who can service
it; Xerox, who purchased the Tektronix printer line, is not providing
service for this printer.

I wonder if any of you who have this printer have a service source?
We seek a service center in the
Oklahoma-Texas-Kansas-Arkansas-Misouri area, but are nearly desperate
enough to ship this thing anywhere in the U.S.

Thanks, in advance, for any help you provide.

Winton Cornell


--

Winton C. Cornell, Ph.D.
Department of Geosciences
College of Engineering and Natural Sciences
The University of Tulsa
Tulsa, OK 74104
phone: 918-631-3248
fax: 918-631-2091
e-mail: winton-cornell-at-utulsa.edu




From daemon Fri Jan 5 03:21:25 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Fri, 5 Jan 2001 04:12:33 -0500
Subject: SEM scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Sorry I do not know the sizes of the scintillators in the 4500/4700 SEM but
the only change you should see is that you run the "contrast" at a lower
level than you did prior to the change.

Work we have carried out suggests an improvement compared with the
conventional product. Scintilltors start when new with a high signal that
falls off quite quickly to start with then there is a gradual decline over
the life of the scintillator. In my mind people do not change, or in your
future case clean, the scintilltors enough.

Good luck

Steve Chapman
Senior Consultant Protrain
For consultancy and training by professionals World Wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com


From daemon Fri Jan 5 03:22:50 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 5 Jan 2001 09:17:06 +0000 (GMT Standard Time)
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's my favourite EM on TV story.

In an episode of the "X-Files" the EM operator looks up
from the TEM and shows Scully the alien virus. It turns
out to be an SEM of a pollen grain.

After telling the story to visitors I remind them that any
scientist who reveals important information to either
Mulder or Scully must take great care when driving home!

Dave


On Wed, 03 Jan 2001 14:25:03 -0600 Warren E Straszheim
{wesaia-at-iastate.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Anyone else happen to watch "Mysterious Ways" earlier this week. One
} character was chatting with another about the fantastic new TEM they were
} using with the "Kevex EDS system with the twin Quantum 10-mm2 detectors". I
} had to listen quick to hear it.
}
} Granted, its a bit dated (Thermo-Noran is now the name and they don't list
} the Quantum on their web page), and some of their other references to other
} techniques were of questionable accuracy. Still, I was tickled to see a
} reference to EM on TV. I remember a few references on "Quincy", but not a
} lot since.
}
} Disclaimer - I don't have a twin Quantum system, but am still using a
} 10-mm2 Quantum on our JEOL-840.
}
} Warren S.
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jan 5 03:25:04 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 5 Jan 2001 09:21:24 +0000 (GMT Standard Time)
Subject: Re: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps you could get a lab with no problems to make some
knives with your glass and then test them in their lab and
yours (get them to send some of their glass to you as
well to test your knifemaker). That should show if it is
the glass, the knifemaker or the ultramicrotome that is at
fault.

Dave


On Wed, 3 Jan 2001 08:04:10 -0800 "Brownell, Patrick"
{patrick.brownell-at-weyerhaeuser.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My problem is a poor knife edge. The knife looks fine under casual
} observation, or with small magnification, but when it comes time to actually
} do some sectioning, I get a lot of chattering, or streaking, or both. I
} know make as many test blocks as I do sample blocks, so I don't waste
} samples with all the bad glass knifes. I've gone through several boxes of
} glass over the past two months, and only get a useable knife every 15 to 20
} breaks. The same knife breaker has worked fine in the past, and I have
} changed the scoring wheel, and checked all that I can.
}
} This knife breaker is also very hard to get parts for. The only obvious
} problem is that one of the pressure points has a rubber pad that is unevenly
} worn. I don't see how that would cause my problem. Again, the knifes look
} good to the naked eye.
}
} The glass I use is from Electron Microscopy Sciences. They are called ultra
} glass knife strips 6.5mm x 25mm x 400 mm.
}
} I guess I'm just looking for someone who has had a similar problem, and
} fixed it in some manner. Could I have gotten a bad batch of glass? I don't
} think it is very likely, I've used several boxes, but they may all be from
} the same batch, for all I know. Is there another brand of glass, or breaker
} that others have found produce reliable knives?
}
} Thanks for any and all help
}
} -Patrick Brownell
} } ----------
} } From: Katja M. Wolski[SMTP:kwolski-at-hsc.usf.edu]
} } Reply To: kwolski-at-hsc.usf.edu
} } Sent: Wednesday, January 03, 2001 4:26 AM
} } To: Brownell, Patrick
} } Subject: Re: Ralph Glass Knife problems
} }
} } I use a different knife machine, but what's the problem you're having?
} } Are you
} } not getting a good knife edge or what?
} }
} } Katja
} }
} }
} }
} } "Brownell, Patrick" wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Hi all!
} } }
} } } I use Ralph type glass knives for plastic sectioning of biological
} } samples.
} } } I am experiencing problems in making a proper knife. I have a TAAB
} } } histoknifemaker (also says Reichart-Jung). Is there anyone willing to
} } offer
} } } expertise in this area?
} } }
} } } -Patrick Brownell
} }
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jan 5 07:33:49 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 5 Jan 2001 05:28:34 -0800 (PST)
Subject: Re: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick:

I don't know the machine, but is TAAB no longer in business in the UK? For
the longest time TAAB supplies (altho not generally easily available in the
US) was an excellent source of resins and small items. Regardless, I would
think that the worn rubber pad is your problem. Again, thinking of
experience with other knifemakers, the pads are an essential part of
relieving some of the stress in the glass as it breaks. If this pad is
uneven, it may not be absorbing the pressure/stress as it should. Perhaps
(if TAAB is not around) some other users of the knifemaker might have
accessories (like a spare pad) to help you out. Also check the following:
depth of the score--if it's too deep or too light, that will affect the
break and edge quality; how fast are you breaking after scoring? - a slow
break has always been essential to consistently good knife edges for me;
check the setting of the clamp(s) and pressure points to ensure that there
are no uneven points and that the clamp(s) are providing equal pressure. I
can't find the reference right now, but the original publications on making
the Ralph knives by hand (it was in Stain Technology, I think) might get you
by, and it also might allow you to check the quality of the glass. The
technique wasn't too difficult or tedious, and, as I remember (from the dim
recesses of ancient history) the percentage of good knives was acceptable.
Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Fri, 5 Jan 2001 09:21:24 +0000 (GMT Standard Time), Patton, David wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Perhaps you could get a lab with no problems to make some
} knives with your glass and then test them in their lab and
} yours (get them to send some of their glass to you as
} well to test your knifemaker). That should show if it is
} the glass, the knifemaker or the ultramicrotome that is at
} fault.
}
} Dave
}
}
} On Wed, 3 Jan 2001 08:04:10 -0800 "Brownell, Patrick"
} {patrick.brownell-at-weyerhaeuser.com} wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } My problem is a poor knife edge. The knife looks fine under casual
} } observation, or with small magnification, but when it comes time to
actually
} } do some sectioning, I get a lot of chattering, or streaking, or both.
I
} } know make as many test blocks as I do sample blocks, so I don't waste
} } samples with all the bad glass knifes. I've gone through several boxes
of
} } glass over the past two months, and only get a useable knife every 15
to 20
} } breaks. The same knife breaker has worked fine in the past, and I have
} } changed the scoring wheel, and checked all that I can.
} }
} } This knife breaker is also very hard to get parts for. The only
obvious
} } problem is that one of the pressure points has a rubber pad that is
unevenly
} } worn. I don't see how that would cause my problem. Again, the knifes
look
} } good to the naked eye.
} }
} } The glass I use is from Electron Microscopy Sciences. They are called
ultra
} } glass knife strips 6.5mm x 25mm x 400 mm.
} }
} } I guess I'm just looking for someone who has had a similar problem, and
} } fixed it in some manner. Could I have gotten a bad batch of glass? I
don't
} } think it is very likely, I've used several boxes, but they may all be
from
} } the same batch, for all I know. Is there another brand of glass, or
breaker
} } that others have found produce reliable knives?
} }
} } Thanks for any and all help
} }
} } -Patrick Brownell
} } } ----------
} } } From: Katja M. Wolski[SMTP:kwolski-at-hsc.usf.edu]
} } } Reply To: kwolski-at-hsc.usf.edu
} } } Sent: Wednesday, January 03, 2001 4:26 AM
} } } To: Brownell, Patrick
} } } Subject: Re: Ralph Glass Knife problems
} } }
} } } I use a different knife machine, but what's the problem you're
having?
} } } Are you
} } } not getting a good knife edge or what?
} } }
} } } Katja
} } }
} } }
} } }
} } } "Brownell, Patrick" wrote:
} } }
} } } }
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
-----------------------------------------------------------------------.
} } } }
} } } } Hi all!
} } } }
} } } } I use Ralph type glass knives for plastic sectioning of biological
} } } samples.
} } } } I am experiencing problems in making a proper knife. I have a TAAB
} } } } histoknifemaker (also says Reichart-Jung). Is there anyone willing
to
} } } offer
} } } } expertise in this area?
} } } }
} } } } -Patrick Brownell
} } }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Fri Jan 5 07:58:14 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 5 Jan 2001 13:54:43 +0000 (GMT Standard Time)
Subject: Re: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TAAB still exist. Try E-mail sales-at-taab.co.uk

Dave

On Fri, 5 Jan 2001 05:28:34 -0800 (PST) Roger Moretz
{rcmoretz-at-excite.com} wrote:

} Patrick:
}
} I don't know the machine, but is TAAB no longer in business in the UK? For
} the longest time TAAB supplies (altho not generally easily available in the
} US) was an excellent source of resins and small items. Regardless, I would
} think that the worn rubber pad is your problem. Again, thinking of
} experience with other knifemakers, the pads are an essential part of
} relieving some of the stress in the glass as it breaks. If this pad is
} uneven, it may not be absorbing the pressure/stress as it should. Perhaps
} (if TAAB is not around) some other users of the knifemaker might have
} accessories (like a spare pad) to help you out. Also check the following:
} depth of the score--if it's too deep or too light, that will affect the
} break and edge quality; how fast are you breaking after scoring? - a slow
} break has always been essential to consistently good knife edges for me;
} check the setting of the clamp(s) and pressure points to ensure that there
} are no uneven points and that the clamp(s) are providing equal pressure. I
} can't find the reference right now, but the original publications on making
} the Ralph knives by hand (it was in Stain Technology, I think) might get you
} by, and it also might allow you to check the quality of the glass. The
} technique wasn't too difficult or tedious, and, as I remember (from the dim
} recesses of ancient history) the percentage of good knives was acceptable.
} Hope this helps.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals, Inc.
}
} On Fri, 5 Jan 2001 09:21:24 +0000 (GMT Standard Time), Patton, David wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Perhaps you could get a lab with no problems to make some
} } knives with your glass and then test them in their lab and
} } yours (get them to send some of their glass to you as
} } well to test your knifemaker). That should show if it is
} } the glass, the knifemaker or the ultramicrotome that is at
} } fault.
} }
} } Dave
} }
} }
} } On Wed, 3 Jan 2001 08:04:10 -0800 "Brownell, Patrick"
} } {patrick.brownell-at-weyerhaeuser.com} wrote:
} }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } My problem is a poor knife edge. The knife looks fine under casual
} } } observation, or with small magnification, but when it comes time to
} actually
} } } do some sectioning, I get a lot of chattering, or streaking, or both.
} I
} } } know make as many test blocks as I do sample blocks, so I don't waste
} } } samples with all the bad glass knifes. I've gone through several boxes
} of
} } } glass over the past two months, and only get a useable knife every 15
} to 20
} } } breaks. The same knife breaker has worked fine in the past, and I have
} } } changed the scoring wheel, and checked all that I can.
} } }
} } } This knife breaker is also very hard to get parts for. The only
} obvious
} } } problem is that one of the pressure points has a rubber pad that is
} unevenly
} } } worn. I don't see how that would cause my problem. Again, the knifes
} look
} } } good to the naked eye.
} } }
} } } The glass I use is from Electron Microscopy Sciences. They are called
} ultra
} } } glass knife strips 6.5mm x 25mm x 400 mm.
} } }
} } } I guess I'm just looking for someone who has had a similar problem, and
} } } fixed it in some manner. Could I have gotten a bad batch of glass? I
} don't
} } } think it is very likely, I've used several boxes, but they may all be
} from
} } } the same batch, for all I know. Is there another brand of glass, or
} breaker
} } } that others have found produce reliable knives?
} } }
} } } Thanks for any and all help
} } }
} } } -Patrick Brownell
} } } } ----------
} } } } From: Katja M. Wolski[SMTP:kwolski-at-hsc.usf.edu]
} } } } Reply To: kwolski-at-hsc.usf.edu
} } } } Sent: Wednesday, January 03, 2001 4:26 AM
} } } } To: Brownell, Patrick
} } } } Subject: Re: Ralph Glass Knife problems
} } } }
} } } } I use a different knife machine, but what's the problem you're
} having?
} } } } Are you
} } } } not getting a good knife edge or what?
} } } }
} } } } Katja
} } } }
} } } }
} } } }
} } } } "Brownell, Patrick" wrote:
} } } }
} } } } }
} ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} -----------------------------------------------------------------------.
} } } } }
} } } } } Hi all!
} } } } }
} } } } } I use Ralph type glass knives for plastic sectioning of biological
} } } } samples.
} } } } } I am experiencing problems in making a proper knife. I have a TAAB
} } } } } histoknifemaker (also says Reichart-Jung). Is there anyone willing
} to
} } } } offer
} } } } } expertise in this area?
} } } } }
} } } } } -Patrick Brownell
} } } }
} } }
} }
} } ----------------------------------------
} } Patton, David
} } Email: David.Patton-at-uwe.ac.uk
} } "University of the West of England"
} }
} }
}
}
}
}
}
} _______________________________________________________
} Send a cool gift with your E-Card
} http://www.bluemountain.com/giftcenter/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jan 5 08:00:48 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Fri, 5 Jan 2001 08:57:43 -0500
Subject: X-TEM of those hydration treated glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Calvin,

Are you vacuum embedding the glass and Spurr's resin? That should work to
get a lot of the void space out.

Just pour the resin into the embedding capsule, put the capsule into a
vacuum oven, let pump down for a few minutes (~20 min), bring the sample
back to room pressure to let the air pressure force the resin into the
pores, then pump it down again and bring back to room pressure and let the
sample cure.

dz

David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Fri Jan 5 08:03:54 2001



From: epmalab :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 5 Jan 2001 06:25:19 -0800
Subject: RE: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris writes ...}

} As part of the planned makeover of our building, it has been
} proposed ...
} Part of that proposal was to set up a computing lab with
} fileserver and work-stations networked ...
} However, there is great pressure on space in this building, and it
} has been suggested to me that computing for imaging and
} spectroscopy does not need to be separated from general purpose
} computing these days. ...

Somewhat true ... for our EPMA and SEM images, we simply archive to a
"files available" location, and every researcher has their own computer and
software. However, you would need a workstation for the OM, would you not?

shAf :o)



From daemon Fri Jan 5 08:20:54 2001



From: us004118-at-mindspring.com ()
Date: Fri, 5 Jan 2001 08:16:23 -0600
Subject: Ask-A-Microscopist: LM: Crystal Growth

Contents Retrieved from Microscopy Listserver Archives
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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------

Email: us004118-at-mindspring.com
Name: Leonard Lessin, FBPA

School: (Retired Science & Medical Photographer)

State: NY

Zip: 10012

Question: I am enjoying doing photomicrographs of crystal
preperations in polarized light.However I have insuffient knowledge of
chemistry
to choose solvents without a long series of trial and error efforts.Can you
give me
a rationale and/or a reference to go about this in a more productive
manner?

---------------------------------------------------------------------------




From daemon Fri Jan 5 09:04:09 2001



From: Alexander Solodukhin :      as4j-at-virginia.edu
Date: Fri, 05 Jan 2001 09:58:58 -0500
Subject: Re: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
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Dear Catherine,

High quality professional film scanners (LeafScan 45, LS-4500 etc.) are very expansive - over $10k.

I use MINOLTA Dimage Scan Multi (Minolta USA -- how2scan.com ) for digitizing of TEM negatives.

Its features:
- Optical resolution: 1128dpi (TEM) 2820dpi (35mm, APS)
- Dynamic range: 3.6
- Price: around $2,000

It has one drawback only: TEM film adapter aperture size is of 2.2x3.15. However only central part of a negative is used for image processing.

There is a choice among the price and convenience.

Best regards,

Alexander

Dr. A.S. Solodukhin
Department of Anesthesiology
University of Virginia Health System
P.O. Box 800710
Charlottesville, VA 22906-0710
FAX : (804) 982-0019
Phone: (804) 924-2494



"Dr. Catherine Powell" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are a clinical diagnostic TEM laboratory serving a provincial network of hospitals. We would like to move away from conventional darkroom printing of images and wish to convert negatives to digital files. We are looking for a negative scanner that will:
}
} 1. Accept 3 X 4 1/4 inch sheet film negatives (Kodak 4489 film).
}
} 2. Comes with software support that is adaptable to a Windows format.
}
} 3. Must be priced under $3000.
}
} 4. Must provide the best quality resolution for diagnostic results (i.e. 3200 X 3600 pixel; wide linear dynamic range).
}
} I look forward to any information and advice you can provide.
}
} Catherine Powell
}
}



From daemon Fri Jan 5 10:15:48 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 05 Jan 2001 10:08:27 -0600
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
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Since there has been only one other response, I will toss in my 2 cents.

Some time back I would have said that the load of microscopic images would
be more than what the general purpose computing lab would want to take
responsibility for. It might prove a significant network traffic load too.
However, I think any decent network should be able to handle the load from
the microscope labs. On the side, we have a good campus network here at
Iowa State. However an analysis of network traffic shows a disproportionate
amount of traffic coming out of a few dorm rooms (the normal traffic
pattern would be incoming). The university is going to start limiting
traffic to so many gigabytes per month or start charging. So I think the
microscope load is no longer that significant.

The other issue that might work against it is software licensing. I don't
know if you use special programs to work with your images. It might be
difficult to work out a suitable licensing arrangement if that software
needs to be installed on a lab full of computers, but maybe you can.

As far as regular maintenance of the data, I would be all in favor of
letting someone else do the backups providing they have the space (and they
should).

Warren

At 06:25 PM 1/2/2001 +0000, you wrote:

} As part of the planned makeover of our building, it has been
} proposed that the various and somewhat distributed microscopy
} facilities in our building - SEM, TEM, Confocal, fluorescence,
} luminescence imaging, darkroom etc. etc. might be brought together
} to form a biological imaging facility, thereby benefitting from some
} improvements in supervision, security and user support. Part of that
} proposal was to set up a computing lab with fileserver and work-
} stations networked to the instruments and dedicated to image
} storage and off-line image and spectral processing and analysis.
} However, there is great pressure on space in this building, and it
} has been suggested to me that computing for imaging and
} spectroscopy does not need to be separated from general purpose
} computing these days. What are your opinions about this? Pro or
} con?
}
} Happy New Millennium
} Chris
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Jan 5 10:30:03 2001



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Fri, 05 Jan 2001 08:26:31 -0800
Subject: request for carbon coater

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

we're looking to buy a used carbon evaporator and before I
speed through the used equipment websites I thought I'd just check to see if
anyone here is thinking of selling their one.

cheers
Liz McKenzie


*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************


From daemon Fri Jan 5 12:05:20 2001



From: Platek, Frank :      FPLATEK-at-ora.fda.gov
Date: Fri, 5 Jan 2001 12:57:27 -0500
Subject: Scanning Microscopy in Forensic Science Session - SCANNING 2001

Contents Retrieved from Microscopy Listserver Archives
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Applications of Scanning Microscopy in Forensic Science


Dear Fellow Microscopist / Forensic Scientist,


SCANNING 2001, the Thirteenth Annual International Scientific Meeting on
Scanning Microscopies will be held May 5-7, 2001, in New York City at The
Roosevelt Hotel. Please make plans to attend three full days of scientific
papers and six short courses all devoted to the use of scanning microscopy.
Numerous specific application topics including forensics, electron
backscattered diffraction, scanning probe microscopy, nanotechnology,
anthropology, modern optical microscopy, museum applications of SEM, food
microstructure, material science, microwave techniques and pharmaceuticals.
For a completely listing of session topics, short course titles and
registration information, please visit the SCANNING web site at
www.scanning-fams.org.

CALL FOR PAPERS:

As a co-chair of the SCANNING 2001's "Applications of Scanning Microscopy in
Forensic Science" session, I am most pleased with the participation and
interest in the forensic session over the last eight years. This year, Mr.
Dennis Ward of the Federal Bureau of Investigation, has graciously agreed to
co-chair the forensic sessions. Together, we are planning to provide an
exciting and informative forensic program. The continued growth of the
forensic session over the last eight years will once again permit two full
days of forensic papers. The "Applications of Scanning Microscopy in
Forensic Science" sessions will be held on Sunday and Monday, May 6-7, 2001.
Combined with the popular one day "Scanning Microscopy in Forensic Science"
short course (Saturday, May 5, 2001), the forensic scientist/student will be
able to attend three consecutive (and full) days of instruction as well as
scientific papers on current forensic science research and unique cases all
devoted to scanning microscopy applications in forensic science. A large
number of microscopy vendors and a full schedule of social activities and
tours in New York are also available for a most enjoyable meeting
experience.

I encourage you to submit an abstract for platform or poster consideration
and be a part of the SCANNING 2001 Forensics Session. Additionally, if you
are involved with or know of forensic science students actively engaged in
forensic research using any type of scanning microscopy, I strongly
encourage you to have your student(s) submit an abstract for consideration
as a student paper or poster. Again, please visit the SCANNING web site at
www.scanning-fams.org.


Should you have any questions about the forensic symposium, short course or
student papers, please feel free to contact me.

See you in New York!


S. Frank Platek, MS
Co-Chair, Forensic Symposium and Short Course
SCANNING 2001
(513) 679-2700
(513) 679-2761 FAX
fplatek-at-ora.fda.gov




From daemon Fri Jan 5 12:23:20 2001



From: OCONNELL-at-ltu.edu
Date: Fri, 05 Jan 2001 13:19:55 -0500 (EST)
Subject: Job Posting

Contents Retrieved from Microscopy Listserver Archives
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Looking for a few good Account Representatives for an electron
and optical microscopy service. Territory is open. Excellent
earning potential. Call Dick O'Connell at 734-668-3309
or e-mail at oconnell-at-ltu.edu for more information.

Thank You

Dick O'Connell


From daemon Fri Jan 5 13:10:16 2001



From: William A. Monroe :      monroe-at-emcenter.msstate.edu
Date: Fri, 5 Jan 2001 14:39:11 -0600
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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I believe TAAB is still in business
TAAB Laboratories Equipment Ltd.
3 Minerva House Calleva Park
Aldermaston , Berkshire
RG7 8NA UK Phone: 44-118-9817775 Fax: 44-118-9817881
E-mail: sales-at-taab.co.uk

Chris


----- Original Message -----
} From: "Roger Moretz" {rcmoretz-at-excite.com}
To: "Patton, David" {David.Patton-at-uwe.ac.uk} ; "Brownell, Patrick"
{patrick.brownell-at-weyerhaeuser.com}
Cc: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 05, 2001 1:28 PM


The earliest mention of EM in TV or movies goes back in time much
further than "Quincy" or the "X-Files".

I thought I would mention my favorite EM TV/Movie memory. Anyone
remember the movie called "The Andromeda Strain" ?? The movie from
the 60's was based on a Michael Crighton book by the same name . A
Transmission Electron Microscope (RCA EMU ???), as well as "live"
images of the ultramicrotomy sectioning process were featured.

Remember?

I would be interested in knowing if there are earlier references than this one.

Best Wishes,

Bill Monroe
--
Bill Monroe
EM Center
Mississippi State University
(601)-325-3019 Lab
Fax 325-0246


From daemon Fri Jan 5 15:21:42 2001



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 05 Jan 2001 15:19:10 -0500
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill:

You're correct about the RCA microscope, except that the one in the
movie was right after RCA discontinued the business, and it was
picked up by "Forgflo", which was the name seen clearly in the movie.

Check it out...

Larry
(Master of all trivia...why don't I get on Who Wants to be a Millionaire?) ;-)




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The earliest mention of EM in TV or movies goes back in time much
} further than "Quincy" or the "X-Files".
}
} I thought I would mention my favorite EM TV/Movie memory. Anyone
} remember the movie called "The Andromeda Strain" ?? The movie from
} the 60's was based on a Michael Crighton book by the same name . A
} Transmission Electron Microscope (RCA EMU ???), as well as "live"
} images of the ultramicrotomy sectioning process were featured.
}
} Remember?
}
} I would be interested in knowing if there are earlier references
} than this one.
}
} Best Wishes,
}
} Bill Monroe
} --
} Bill Monroe
} EM Center
} Mississippi State University
} (601)-325-3019 Lab
} Fax 325-0246

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Fri Jan 5 15:45:18 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 05 Jan 2001 16:43:15 -0500
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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You might also recall that in the Andromeda Strain movie, the specimen was
inserted through the viewing port and placed on the fluorescent screen. In
this way the were actually able to watch the "virus" replicate right before
their eyes.

At 02:39 PM 01/05/2001 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251


From daemon Fri Jan 5 15:56:46 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 5 Jan 2001 15:53:58 -0600
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Warren E Straszheim" {wesaia-at-iastate.edu}
{snip}

}
} As far as regular maintenance of the data, I would be all in favor of
} letting someone else do the backups providing they have the space (and
they
} should).

Warren,

If the backups are of any value to you the only person you can trust to
make them is yourself. The only method I have found that has never failed
me it to compress the files in zip format transfer it to the backup media
and verify it on the back up media. Then store the back up media off site.
The zip format applies to MSDOS computer other archive file types apply to
other OSs.

If the data is really important make two copies.

Unless you can hire a lot better folks then I have seen colleges hire they
will make mistakes on backups sooner or later probably sooner.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Fri Jan 5 17:00:30 2001



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Fri, 05 Jan 2001 14:56:02 -0800
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Of course some of us remember this movie but for heavens sake why did they have green "colored" (interference "color") sections floating in the microtome knife boat when gold surely would have photographed as well and fit the dialogue of that scene. Obviously the technical people just said well cut us some sections to phtograph. A sore point to me at the time.

} } } "William A. Monroe" {monroe-at-emcenter.msstate.edu} 01/05 12:39 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The earliest mention of EM in TV or movies goes back in time much
further than "Quincy" or the "X-Files".

I thought I would mention my favorite EM TV/Movie memory. Anyone
remember the movie called "The Andromeda Strain" ?? The movie from
the 60's was based on a Michael Crighton book by the same name . A
Transmission Electron Microscope (RCA EMU ???), as well as "live"
images of the ultramicrotomy sectioning process were featured.

Remember?

I would be interested in knowing if there are earlier references than this one.

Best Wishes,

Bill Monroe
--
Bill Monroe
EM Center
Mississippi State University
(601)-325-3019 Lab
Fax 325-0246




From daemon Fri Jan 5 17:01:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 5 Jan 2001 17:58:37 -0500
Subject: RE: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The other fun thing about the crystalline image in that TEM from Andromeda Strain was that it was in color!


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: William A. Monroe [mailto:monroe-at-emcenter.msstate.edu]
Sent: Friday, January 05, 2001 3:39 PM
To: Warren E Straszheim
Cc: Microscopy-at-sparc5.microscopy.com
Subject: Re: TV Trivia


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html



---------------------------------------------------------------
--------.


The earliest mention of EM in TV or movies goes back in time much
further than "Quincy" or the "X-Files".

I thought I would mention my favorite EM TV/Movie memory. Anyone
remember the movie called "The Andromeda Strain" ?? The movie from
the 60's was based on a Michael Crighton book by the same name . A
Transmission Electron Microscope (RCA EMU ???), as well as "live"
images of the ultramicrotomy sectioning process were featured.

Remember?

I would be interested in knowing if there are earlier
references than this one.

Best Wishes,

Bill Monroe
--
Bill Monroe
EM Center
Mississippi State University
(601)-325-3019 Lab
Fax 325-0246



From daemon Fri Jan 5 18:31:25 2001



From: Richard Wuhrer :      Richard.Wuhrer-at-uts.edu.au
Date: Fri, 5 Jan 2001 18:26:11 -0600
Subject: AMAS Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_________________________________________________________________________

"Scanned Probe and Electron Microscopy and Microanalysis: Applications &
Techniques"
_________________________________________________________________________

The University of Sydney

February 14-16, 2001


AMAS and ASPMS invite you to join us for what will probably be the very
first electron and scanned probe microscopy meeting of the 21st Century.

The aim of the Symposium is to provide a forum where participants can
discuss electron and scanned probe microscopy, with emphasis on practical
solutions and applications. A number of overseas invited speakers will be
involved with both the Symposium and the Workshops. We also encourage you
to present your recent work using SPM, SEM/TEM and microanalysis.

Pre-Symposium Workshop
February 12-13, 2001
Practical Digital Imaging Introduction to SPM
Spectral Imaging Materials SPM
Environmental SEM Biological SPM
Low Voltage SEM and microanalysis SPM of Soft Materials
Advances in EBSD in SEM SPM Calibration and Maintenance
Introduction to XEDS and EELS in the EM Functionalising Tips

Information concerning the symposium, workshops, accommodation,
registration, etc. is posted on our website (www.microscopy.org.au then
follow the link to "Upcoming ASPMS, AMAS Symposium". This information will
be revised and expanded as necessary.

General Enquiries:
Clive Nockolds, Tel. (02) 9351 2351, fax (02) 9351 7682, email:
clive-at-emu.usyd.edu.au

SPM Enquiries:
Gordon Thorogood, Tel. (02) 9717 3183, fax (02) 9543 7179, email:
gjt-at-ansto.gov.au

Website:
http://www.microscopy.org.au/symposium/Symposium_HomePage.html




**************************************************
Richard Wuhrer
Microstructural Analysis Unit
Faculty of Science
University of Technology, Sydney
Lower Ground Floor, Bldg 4, Thomas Street, Ultimo
PO Box 123 Broadway NSW 2007
+61 2 9514 1702 (P) +61 2 9514 1703 (fax)
0411 877 476 (M)
Email: Richard.Wuhrer-at-uts.edu.au
**************************************************





From daemon Fri Jan 5 19:20:24 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 Jan 2001 17:16:06 -0800
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a word of caution about Zip disks. They can be very
unreliable. When Iomega first came out with them, their
media was really good. Now it is not the same. Maxell
and Fujifilm also make media. I think that theirs are better.

The other problem with Iomega drives (Zip and Jaz) is the
click of death. This is a precursor to a dead drive or
media and loss of all that is on the media (if bad media).

To check your drives and media, run tip.exe. To find out
more about this, visit http://www.grc.com

gary g.


At 01:53 PM 1/5/01, you wrote:

} ----- Original Message -----
} } From: "Warren E Straszheim" {wesaia-at-iastate.edu}
} {snip}
}
} }
} } As far as regular maintenance of the data, I would be all in favor of
} } letting someone else do the backups providing they have the space (and
} they
} } should).
}
} Warren,
}
} If the backups are of any value to you the only person you can trust to
} make them is yourself. The only method I have found that has never failed
} me it to compress the files in zip format transfer it to the backup media
} and verify it on the back up media. Then store the back up media off site.
} The zip format applies to MSDOS computer other archive file types apply to
} other OSs.
}
} If the data is really important make two copies.
}
} Unless you can hire a lot better folks then I have seen colleges hire they
} will make mistakes on backups sooner or later probably sooner.
}
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00



From daemon Fri Jan 5 22:07:44 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 5 Jan 2001 22:07:12 -0600
Subject: Re: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Gordon Couger" {gcouger-at-couger.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 05, 2001 7:16 PM


Could someone post the reference for the article for making glass knives
by hand?

Thanks
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Jan 5 22:51:41 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Sat, 6 Jan 2001 15:52:24 +1000
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


And there were all those Zeiss microscopes in Jurassic Park. Pity the one
they were using in one scene (an Axiophot?) didn't have a condenser....


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Fri Jan 5 23:03:56 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 Jan 2001 20:59:44 -0800
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes....different "zip." Winzip is very good for munching
files into a smaller data space. The media problem remains,
however. As you point out, other media keeps coming out.

I tried DVD-RAM and really got whacked by bad sectors.
I don't use that any longer. DVD-R is quite another story.
CD-R is so cheap these days it is a great way to store
600MB of data. The only problem is to ensure that it
remains on the media. Most burner programs do not
verify after write. Toast on the Mac does, but Adaptec's
programs on the PC do not. I have not found any other
programs that will verify after write on the PC. Bummer.

I have not done much at all with CD-RW. I wonder what
the experiences have been with this option? My new Yamaha
drive is 16x/10x/40x and should do nicely for RW. Never tried
it for RW. I guess that I should do that some day. CD-Rs at
12X are iffy....even with certified media. That's why the
verification is such an important missing feature.

For really good backup and restore, consider the removable
IDE drives. Very nice. My one year's work fits on three 45G
drives. Hopefully it remains accessible in the future. Data
overload is an emerging problem.

gg


At 08:03 PM 1/5/01, you wrote:

} ----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "Gordon Couger" {gcouger-at-couger.com}
} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, January 05, 2001 7:16 PM
} Subject: Re: Computing for microscopy and imaging
}
}
} } Just a word of caution about Zip disks. They can be very
} } unreliable. When Iomega first came out with them, their
} } media was really good. Now it is not the same. Maxell
} } and Fujifilm also make media. I think that theirs are better.
} }
} } The other problem with Iomega drives (Zip and Jaz) is the
} } click of death. This is a precursor to a dead drive or
} } media and loss of all that is on the media (if bad media).
} }
} } To check your drives and media, run tip.exe. To find out
} } more about this, visit http://www.grc.com
} }
} } gary g.
}
} Gary,
}
} Zip files not Zip disk. Use Pkzip or some other program to put all the
} files into one zip file, usually a complete directory, copy that file to
} the back up media and verify the zip file on the back up media. Currently
} I use write only CD-ROMs and keep a copy of really important stuff on my
} internet server at my ISP as well. Hard disk space is cheap. I have the
} last 15 years work at my finger tips. If I can find it:) I started using
} this on single sided single density floppies on a Radio Shack Model 1. I
} can only think of two times that I was unable to retrieve a file in 20
} years.
}
} I am a programmer not a photographer so a years work might fit on a floppy
} disk.
}
} I am not satisfied with the life of CD-ROMs but so far something better
} has always come along before the old media went bad and I copied it all
} over. One thing I don't like about CD-ROMs is they are not protected from
} scratching or being broken.
}
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00



From daemon Sat Jan 6 00:32:59 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sat, 6 Jan 2001 18:01:59 +1100
Subject: JENA ZEISS Terchnoval 2

Contents Retrieved from Microscopy Listserver Archives
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----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Gordon Couger" {gcouger-at-couger.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 05, 2001 10:59 PM


I am looking for accessories and parts for binocular microscope Jenna
Technoval 2.
Anyone have some spare unneeded accessories as camera attachment, oculars,
literature, etc?

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Sat Jan 6 01:21:03 2001



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 6 Jan 2001 01:18:23 -0600
Subject: Re: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In one of the Batman movies where he fights the arch villain Mr. Freeze. In
the part of the move where the police raid Mr. Freezes' lair you will see
off to one side a Philips 400 TEM dressed up as a cheap prop. This is an
insult to the Scientist and Technicians who have developed finely honed
skills to bring to light the world of the very small in a effort to make
life a little better for all of us.
Electron Microscopy has been an important part of Science for the last half
of the 20th century. This finely engineered tool of Science has open up the
world of the very small to eyes that would never have imagined that life
could exist on such a level. It sickens me to see this fine and noble tool
of Science reduced to a carnival side show gimmick. It is my hope that the
men and women of this newsgroup will in their own way and in their own time
bring electron microscopy back to its proper place in the scientific
community, because if we of this newsgroup and others who labor in Science
do not, than this important source of knowledge will be lost to history and
the politicians.
I will now get off my soap box and make no further comment on the subject.

Ronald Austin
Research Associate
LSU Medical Center
Dept of Pathology
Shreveport, LA
rla-at-mindspring.com


-----Original Message-----
} From: Rosemary White [mailto:Rosemary.White-at-pi.csiro.au]
Sent: Friday, January 05, 2001 11:52 PM
To: Microscopy-at-sparc5.microscopy.com


And there were all those Zeiss microscopes in Jurassic Park. Pity the one
they were using in one scene (an Axiophot?) didn't have a condenser....


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au






From daemon Sat Jan 6 05:44:51 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 6 Jan 2001 05:39:49 -0600
Subject: RE: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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Ah, come on. There were many technical errors in that film, but wasn't it
fun? One of my favorite faux pas was the rapid growth? multiplication? of
the strain. Not to mention it's apparent ability to degrade a variety of
natural and synthetic rubbers while also attacking human blood plasma.

Michael Crichton did a good job of describing a possible protagonist, and
Hollyweird did its usual job of visualizing it. To expect anything
different would be folly.


On Friday, January 05, 2001 4:59 PM, Walck, Scott D. [SMTP:walck-at-ppg.com]
wrote:
}
} The other fun thing about the crystalline image in that TEM from
Andromeda Strain was that it was in color!
}
}
} -Scott
}
} -----Original Message-----
} From: William A. Monroe [mailto:monroe-at-emcenter.msstate.edu]

}
} The earliest mention of EM in TV or movies goes back in time much
} further than "Quincy" or the "X-Files".
}
} I thought I would mention my favorite EM TV/Movie memory. Anyone
} remember the movie called "The Andromeda Strain" ?? The movie from
} the 60's was based on a Michael Crighton book by the same name . A
} Transmission Electron Microscope (RCA EMU ???), as well as "live"
} images of the ultramicrotomy sectioning process were featured.
}
} Remember?
}
} I would be interested in knowing if there are earlier
} references than this one.
}
} Best Wishes,
}
} Bill Monroe
} --
} Bill Monroe
} EM Center
} Mississippi State University
} (601)-325-3019 Lab
} Fax 325-0246
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sat Jan 6 11:20:51 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 06 Jan 2001 09:12:17 -0800
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
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At 10:29 PM 1/5/01, you wrote:


} } Yes....different "zip." Winzip is very good for munching
} } files into a smaller data space. The media problem remains,
} } however. As you point out, other media keeps coming out.
} ============================
} I really think the best media out there for long term storage would be
} plain old 35 mm film. I don't know what kind of data density you could get
} on tech pan but I would trust the data to be there when I wanted to get it
} back.

Winzip does a good job on data that is compressible. Non-LZW TIFF
can be compressed as can ASCII and other byte hungry data. But
I don't find much size reduction with compressed TIFF or JPEG. These
are mostly what I have. Even then, my native images are uncompressed
TIFF. Those are what I need to save as backups plus backups of the
backups.

} }
} } I have not done much at all with CD-RW. I wonder what
} } the experiences have been with this option? My new Yamaha
} } drive is 16x/10x/40x and should do nicely for RW. Never tried
} } it for RW. I guess that I should do that some day. CD-Rs at
} } 12X are iffy....even with certified media. That's why the
} } verification is such an important missing feature.
} ===============
} } From the reports I see on RW CDROMs the storage life is a lot shorter than
} a R CDROM. Both of them use some kind of dye process and the RW is
} reversable.

I too have not heard good things about RW. Unless and until the
viability is assured, my precious data isn't going on an RW media.
When its gone, its gone. Not a good situation.


} It takes time and markting didn't like it is my guess. But that is why you
} have to verify each file you copy with verify option of Winzip. It reads
} the file and does the CRC and checks it against the one in the file.
}
} It is slow and cumbersom but it is the only way I have found that works on
} MSDOS or Windos. On Linux or Unix I can write a script to do it all. I
} have been involved in disaster recovereries and most of the times at least
} part of the back ups are bad. Some of the restore system can't recover
} from a bad file. I don't know much about backup systems for contemperary
} systems I just copy every thing to a CDROM uncompressed and put a
} compressed version on the network box and call it good.

Hum. I'm using Winzip 7 and it is very fast on Win98SE. Stuffit on the
Mac also does a nice job.

} }
} } For really good backup and restore, consider the removable
} } IDE drives. Very nice. My one year's work fits on three 45G
} } drives. Hopefully it remains accessible in the future. Data
} } overload is an emerging problem.
} ==================================
} That's what I do on my Linux box on the internet. I have a drive for
} backups. Drives are cheap. High quality digital imagining is going to
} really eat up disk space. We need 10 or 20 gig CDROMs

Yes indeed. Even a 10G CD would be great, if it worked. I've
toyed with the idea of DVD-R which will do 4.7G and 9G. But
the drives are very pricey as is the media. And I suspect they
may have the same reliability issues as CD-RW and even CD-R.
Not all CD-R writes work 100%. Only Toast on the Mac will do
a verify after write. I've not found any burner program for the PC
that will verify after write. Strange.

The IDE drive trays seem like a good approach to backup. The
drives too are obsoleted rather quickly. My "new" IBM 45G Deskstar
ATA-66 drives are now discontinued. IBM makes ATA-100
45G drives for $239 versus the $129 I paid for the ATA-66 ones.

My only other backup option (a redundant one) is a Sony SDT-9000
4mm DAT. Using native hardware compression, it will store 24G
on a DDS-3 120meter tape. But this has some peculiar problems
based on lack of backup software for tape that works in a dual
host adapter environment. Dantz's Retrospect works great on
the Mac but fails on the PC. I have to use old Win95 Adaptec
backup. It works but does not offer user interface to the
drive's features.

It seems that as we rush from film to digital, we may look back
on the thousands of negs or chromes sitting in file cabinets
and wonder what happened. We'd now have file cabinets of
$200 hard drives which may or may not work after sitting for
some amount of time. A rather unsettling feeling.

gary g.



From daemon Sat Jan 6 11:29:44 2001



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Sat, 6 Jan 2001 10:25:58 -0700
Subject: RE: TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Holey Microscopy Batman...maybe we should blame that on Alfred. And to
think I didn't tune in the following night...same bat time...same bat
channel...to see if this oversight was corrected.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Ronald Austin [mailto:rla-at-mindspring.com]
Sent: Saturday, January 06, 2001 12:18 AM
To: Microscopy Society of America


In one of the Batman movies where he fights the arch villain Mr. Freeze. In
the part of the move where the police raid Mr. Freezes' lair you will see
off to one side a Philips 400 TEM dressed up as a cheap prop. This is an
insult to the Scientist and Technicians who have developed finely honed
skills to bring to light the world of the very small in a effort to make
life a little better for all of us.
Electron Microscopy has been an important part of Science for the last half
of the 20th century. This finely engineered tool of Science has open up the
world of the very small to eyes that would never have imagined that life
could exist on such a level. It sickens me to see this fine and noble tool
of Science reduced to a carnival side show gimmick. It is my hope that the
men and women of this newsgroup will in their own way and in their own time
bring electron microscopy back to its proper place in the scientific
community, because if we of this newsgroup and others who labor in Science
do not, than this important source of knowledge will be lost to history and
the politicians.
I will now get off my soap box and make no further comment on the subject.

Ronald Austin
Research Associate
LSU Medical Center
Dept of Pathology
Shreveport, LA
rla-at-mindspring.com







From daemon Sat Jan 6 13:24:40 2001



From: Robert Ruscica :      ruscica-at-etp-usa.com
Date: Sat, 06 Jan 2001 11:19:43 -0800
Subject: Ultimate and perhaps last of TV Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have enjoyed reading all the TV trivia regarding the Quincy and Andromeda
Strain. What many of you may not know is that for the "Strain" movie the
makers of the film contacted a now defunct Microprobe Company, Materials
Analysis Co. We sent to the studio an Electron Microprobe. The only portion
that made the film was some blinking x-ray scalers and a portion of the
electronics rack. What the impact of microprobe was in the film I can't
guess or remember.
In the Quincy episodes, there were more than one, we sent to the studio an
ISI SEM along with a service engineer, whose name at the moment escapes
me, to install it and our application and Demonstration man, Bill Roth to
run the SEM, Bill has been with Hitachi for many years now and could give
more incite as to what happened at the studio than I can as he was there
for a week doing the one episode. I remember him telling me that Quincy's
technician, Sam was explaining to Quincy what was on the CRT with the
camera going from the control panel with Bill's hands being filmed but the
overall shot of Sam and Quincy looking at the CRT and discussing the
forensic material. I still have some 8x10's around here somewhere of the
filming. Thought you might be interested.

Regards,
Bob Ruscica



From daemon Sat Jan 6 22:03:43 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 6 Jan 2001 21:57:46 -0600
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Gary Gaugler" {gary-at-gaugler.com}

{snip}
} Winzip does a good job on data that is compressible. Non-LZW TIFF
} can be compressed as can ASCII and other byte hungry data. But
} I don't find much size reduction with compressed TIFF or JPEG. These
} are mostly what I have. Even then, my native images are uncompressed
} TIFF. Those are what I need to save as backups plus backups of the
} backups.
=========
These files are already compressed as much as possible. The only
advantage of zip programs they get them in one file.
{snip}
} } } For really good backup and restore, consider the removable
} } } IDE drives. Very nice. My one year's work fits on three 45G
} } } drives. Hopefully it remains accessible in the future. Data
} } } overload is an emerging problem.
} } ==================================
} } That's what I do on my Linux box on the internet. I have a drive for
} } backups. Drives are cheap. High quality digital imagining is going to
} } really eat up disk space. We need 10 or 20 gig CD-ROMs
}
} Yes indeed. Even a 10G CD would be great, if it worked. I've
} toyed with the idea of DVD-R which will do 4.7G and 9G. But
} the drives are very pricey as is the media. And I suspect they
} may have the same reliability issues as CD-RW and even CD-R.
} Not all CD-R writes work 100%. Only Toast on the Mac will do
} a verify after write. I've not found any burner program for the PC
} that will verify after write. Strange.
}
} The IDE drive trays seem like a good approach to backup. The
} drives too are obsolete rather quickly. My "new" IBM 45G Deskstar
} ATA-66 drives are now discontinued. IBM makes ATA-100
} 45G drives for $239 versus the $129 I paid for the ATA-66 ones.
=============
I just use a regular IDE drive it's about as cheap and not much more
trouble to change if you put it in the bottom bay and don't screw it in.
That works well for me but it would not work well for large images. They
just generate too much data.
}
} My only other backup option (a redundant one) is a Sony SDT-9000
} 4mm DAT. Using native hardware compression, it will store 24G
} on a DDS-3 120meter tape. But this has some peculiar problems
} based on lack of backup software for tape that works in a dual
} host adapter environment. Dantz's Retrospect works great on
} the Mac but fails on the PC. I have to use old Win95 Adaptec
} backup. It works but does not offer user interface to the
} drive's features.
====================
Be careful reusing tapes. They can only be use a few times before they
start getting unreliable. One I was using recommended 5 reuses.

Tape is also slow to get a single file from. The one I want is always on
the end.
}
} It seems that as we rush from film to digital, we may look back
} on the thousands of negs or chromes sitting in file cabinets
} and wonder what happened. We'd now have file cabinets of
} $200 hard drives which may or may not work after sitting for
} some amount of time. A rather unsettling feeling.

I agree that it is unsettling. That why I have said in the past
photographic film is probably still the best archival media we have from
cost, aging and resolution point of view. It's fatal flaw is lack of
instant availability and knowing if you got a good shot or not while you
are
still set up.

An interesting setup would be a beam splitter that would let you take
either plain old film or CCD images. Then you could do your long term
storage on film that we know won't go obsolete with of the next upgrade
and we have no questions about how long it will last. Film is competitive
or
cheaper with digital storage if you include resolution in the equation.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Sun Jan 7 08:24:35 2001



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Sun, 07 Jan 2001 09:12:19 -0500
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Gary, Gordon & Microscopists !

What I have been doing, for convenience more than anything,
is to copy my SEM & PM prints with a digital camera at 1024
by 768 resolution. I use a copy stand for its light sources
but I hand-hold the camera (!). After all, the images are
already at their maximum enlargement, so I won't be looking
for more detail than can already be seen in the original
image. They print at 200 dpi, not as good as what the printer
can do (600 dpi) but they look fine in my reports, and then
I can use HTML to write the report because the macro images
are in a digital format, too. Extra copies are no longer a
problem; in the "good old days" we (Amenex's microscopist,
that is) had to spend days at a time making contact prints
in a rube-goldberg photo lab set up in the metallographic
preparation room (only one that's dark enough).

On the other hand, when one starts making digitally
recorded images on the SEM, theoretically one is creating
a super-wide-angle image. Does the resolution hold up ?
If not, we're kidding ourselves like the fly on a raft
who wants the drawbridges raised ...

Best regards,
George Langford, Sc.D.
amenex-at-amenex.com
http://www.amenex.com/


From daemon Sun Jan 7 10:19:46 2001



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Sun, 7 Jan 2001 17:28:57 +0100
Subject: need help on X-EDS k factors

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Hi, everybody !

I would greatly appreciate some hints concerning the kind of materials
(minerals ?) that
could be used in order to determine the k(A,B) (Cliff-Lorimer approx.)
factors for my instrument
(TEM CM-200 Philips microscope with EDAX EDS X-ray spectrometer) concerning
Zr(K-lines),
Y(K-lines), Hf(L-lines) and La(?-lines). Do they exist Si containing minerals
which could be
used to determine the k factors relating Si and the above mentioned elements ?
Are they suppliers of standard materials that could be used for determining
the k factors for the
above mentioned elements ?

Thank you in advance !

Corneliu Sarbu, PhD
Dept.of Metallurgy and Applied Materials Science
Catholic University of Leuven
Belgium


From daemon Sun Jan 7 10:27:51 2001



From: jwahom01-at-tufts.edu ()
Date: Sun, 7 Jan 2001 10:23:30 -0600
Subject: Ask-A-Microscopist:TEM of calcium carbonate crystals

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Email: jwahom01-at-tufts.edu
Name: Jane Wahome
School: Tufts University

Question: I need to view calcium carbonate crystals with a transmission
electron microscope for a research project. I also need to record the
images. All I've managed to do so far is order thin carbon grids. I have
been asked to write a plan for my experiment but I have no clue how to
prepare crystals in solution for magnification or what tools to use.
Please give me some direction. My professors say I should figure
everything out myself - "It will be a good learning experience."

---------------------------------------------------------------------------




From daemon Sun Jan 7 11:50:28 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 07 Jan 2001 09:45:09 -0800
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
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I only do digital images on the SEM. There are several
providers of this capability for retrofitting. Some if not
most all current SEM makers include digital imaging.
When running an active scan system, the digital image
is just as on the TV screen and Polaroid output film.
But it can be made different. Since the voltage points
for limits of the scanned frame are user programmable,
a small region can be scanned at very high resolution
or a larger region at a lower resolution. The limiting
factor is the number of bits in the D/A converter which
drives the scan coils. Most of these are 12-bits, so that
makes 4096 discrete positions across the X range
specified and 4096 discrete positions for the Y range.

My highest recording setting is 4032x2688 pixels which
is 10.8M pixels at 8-bits per pixel or twice that at 16-bits
per pixel. The 4032x2688 values are chosen to make
the aspect ratio 1.5:1, so it fully fits a 35mm frame. The normal
1.33:1 does not. Given that the scan limits are set as for
a Polaroid shot, the equivalent pixel density is between
700 and 800 pixels per inch for the digital capture image.
Not bad at all. And since the pixel dwell time is programmable,
one can optimize it for scan time and minimum noise.

gary g.


At 06:12 AM 1/7/01, you wrote:
} Hello Gary, Gordon & Microscopists !
}
} [snip]
} On the other hand, when one starts making digitally
} recorded images on the SEM, theoretically one is creating
} a super-wide-angle image. Does the resolution hold up ?
} If not, we're kidding ourselves like the fly on a raft
} who wants the drawbridges raised ...
}
} Best regards,
} George Langford, Sc.D.
} amenex-at-amenex.com
} http://www.amenex.com/



From daemon Sun Jan 7 16:30:40 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sun, 07 Jan 2001 20:08:50 -0500
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
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----- Original Message -----
} From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
To: "Gordon Couger" {gcouger-at-couger.com}
Cc: {microscopy-at-sparc5.microsocpy.com}
Sent: Sunday, January 07, 2001 8:34 PM


George,
What do you mean by a super-wide-angle picture? The digital scan output
goes through the mag control, so your angle is the same as for Polaroid
at a given mag. The resolution is determined by the pixel density and
won't exceed a Type 55 negative on your ETEC until you capture about 4k
by 4k. 2k by 2k isn't quite as good as you can do with film, the
limiting factor being your record CRT and camera. As Gordon said, film
is still the measure. It lasts, the resolution is great and software
"upgrades" won't make your entire file obsolete.

Besides, don't you have some problems using pure digital imaging for
legal cases? My understanding is that if you use digital, the original
file must be on a WORM drive (now known as CD-R) to give the equivalent
of an original negative on file. This info came from the Virginia
Crime Labs quite a few years ago.

Ken Converse,
owner
Quality Images
Delat, PA


George Langford, Sc.D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Gary, Gordon & Microscopists !
}
} What I have been doing, for convenience more than anything,
} is to copy my SEM & PM prints with a digital camera at 1024
} by 768 resolution. I use a copy stand for its light sources
} but I hand-hold the camera (!). After all, the images are
} already at their maximum enlargement, so I won't be looking
} for more detail than can already be seen in the original
} image. They print at 200 dpi, not as good as what the printer
} can do (600 dpi) but they look fine in my reports, and then
} I can use HTML to write the report because the macro images
} are in a digital format, too. Extra copies are no longer a
} problem; in the "good old days" we (Amenex's microscopist,
} that is) had to spend days at a time making contact prints
} in a rube-goldberg photo lab set up in the metallographic
} preparation room (only one that's dark enough).



}
} On the other hand, when one starts making digitally
} recorded images on the SEM, theoretically one is creating
} a super-wide-angle image. Does the resolution hold up ?
} If not, we're kidding ourselves like the fly on a raft
} who wants the drawbridges raised ...
}
} Best regards,
} George Langford, Sc.D.
} amenex-at-amenex.com
} http://www.amenex.com/
}
}
}



From daemon Sun Jan 7 21:05:21 2001



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Sun, 07 Jan 2001 21:59:52 -0500
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ken & Microscopists !

Ken Converse wrote:

} What do you mean by a super-wide-angle picture? The digital scan
} output goes through the mag control, so your angle is the same as
} for Polaroid at a given mag. The resolution is determined by the
} pixel density and won't exceed a Type 55 negative on your ETEC
} until you capture about 4k by 4k. 2k by 2k isn't quite as good as
} you can do with film, the limiting factor being your record CRT and
} camera.

The image I see on the Polaroid film comes nowhere near taxing the
resolution of the film. My 1024 by 768 pixel digicam snapshots of
the original Polaroid prints look pretty close to the originals.
Why enlarge them any more ? Empty magnification and all that. Yes,
I know that the twelve- or sixteen-bit ADC's can capture a much wider
range of exposure than can film, but I don't see where all those
pixels get us any more spatial resolution. A 35 mm camera can cram
a lot of resolution onto a small area of film, but its lens is
reducing the original scene, not enlarging it. All the fancy lenses
on our metallographs can't do any better than covering the 4X5 inch
format of the film at maximum resolution. If we try to use a smaller
eyepiece to get a wide-angle effect, then the edges of the image show
terrible distortion. So there's no point in using an excessive number
of pixels to describe the output of a microscope's imaging system.

} As Gordon said, film is still the measure. It lasts, the
} resolution is great and software "upgrades" won't make your entire
} file obsolete.

Isn't it the hardware that goes South ? Where do I read my eight-inch
floppies; or the 5-1/4 inch ones, for that matter ... ? Once a bit
map, always a bit map, I should think. I do notice that my .JPG
image editor can't make heads or tails of some of the .JPG files
that look fine on Netscape, so there are some software issues, but
it looks as though the old drives are the real problem.

} Besides, don't you have some problems using pure digital imaging
} for legal cases? My understanding is that if you use digital, the
} original file must be on a WORM drive (now known as CD-R) to give
} the equivalent of an original negative on file. This info came from
} the Virginia Crime Labs quite a few years ago.

I lock the original floppy disk and leave the original image files
untouched there. Then I copy 'em to my hard drive for cropping and
adjustment of contrast, etc. Anyone wants to see the report, gets
the whole shebang: originals, thumbnails, edited pix, and text (HTML).
CDROM's are handy for the monster files that result. One report
had almost a thousand individual files in it. Client wasn't too
happy with the task of learning what a browser is. Printing it all
out is a monumental PIA, but then, so is ruffling through a
two-inch-thick pile of prints.

Best regards,
George Langford
Principal Consultant
Amenex Associates, Inc.
http://www.amenex.com/


From daemon Mon Jan 8 03:59:28 2001



From: Alan Bright :      bright-at-dial.pipex.com
Date: Mon, 8 Jan 2001 09:50:13 -0000
Subject: Re: RE: Ralph Glass Knife problems

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I can assure you all that TAAB is well and truly still in business as we
communicate on a regular basis concerning cryostats and microtomes. Please
contact TAAB on: sales-at-taab.co.uk

Best. Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com


-----Original Message-----
} From: Roger Moretz [mailto:rcmoretz-at-excite.com]
Sent: 05 January 2001 13:29
To: Patton, David; Brownell, Patrick
Cc: 'Microscopy-at-MSA.Microscopy.Com'


Patrick:

I don't know the machine, but is TAAB no longer in business in the UK? For
the longest time TAAB supplies (altho not generally easily available in the
US) was an excellent source of resins and small items. Regardless, I would
think that the worn rubber pad is your problem. Again, thinking of
experience with other knifemakers, the pads are an essential part of
relieving some of the stress in the glass as it breaks. If this pad is
uneven, it may not be absorbing the pressure/stress as it should. Perhaps
(if TAAB is not around) some other users of the knifemaker might have
accessories (like a spare pad) to help you out. Also check the following:
depth of the score--if it's too deep or too light, that will affect the
break and edge quality; how fast are you breaking after scoring? - a slow
break has always been essential to consistently good knife edges for me;
check the setting of the clamp(s) and pressure points to ensure that there
are no uneven points and that the clamp(s) are providing equal pressure. I
can't find the reference right now, but the original publications on making
the Ralph knives by hand (it was in Stain Technology, I think) might get you
by, and it also might allow you to check the quality of the glass. The
technique wasn't too difficult or tedious, and, as I remember (from the dim
recesses of ancient history) the percentage of good knives was acceptable.
Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Fri, 5 Jan 2001 09:21:24 +0000 (GMT Standard Time), Patton, David wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Perhaps you could get a lab with no problems to make some
} knives with your glass and then test them in their lab and
} yours (get them to send some of their glass to you as
} well to test your knifemaker). That should show if it is
} the glass, the knifemaker or the ultramicrotome that is at
} fault.
}
} Dave
}
}
} On Wed, 3 Jan 2001 08:04:10 -0800 "Brownell, Patrick"
} {patrick.brownell-at-weyerhaeuser.com} wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } My problem is a poor knife edge. The knife looks fine under casual
} } observation, or with small magnification, but when it comes time to
actually
} } do some sectioning, I get a lot of chattering, or streaking, or both.
I
} } know make as many test blocks as I do sample blocks, so I don't waste
} } samples with all the bad glass knifes. I've gone through several boxes
of
} } glass over the past two months, and only get a useable knife every 15
to 20
} } breaks. The same knife breaker has worked fine in the past, and I have
} } changed the scoring wheel, and checked all that I can.
} }
} } This knife breaker is also very hard to get parts for. The only
obvious
} } problem is that one of the pressure points has a rubber pad that is
unevenly
} } worn. I don't see how that would cause my problem. Again, the knifes
look
} } good to the naked eye.
} }
} } The glass I use is from Electron Microscopy Sciences. They are called
ultra
} } glass knife strips 6.5mm x 25mm x 400 mm.
} }
} } I guess I'm just looking for someone who has had a similar problem, and
} } fixed it in some manner. Could I have gotten a bad batch of glass? I
don't
} } think it is very likely, I've used several boxes, but they may all be
from
} } the same batch, for all I know. Is there another brand of glass, or
breaker
} } that others have found produce reliable knives?
} }
} } Thanks for any and all help
} }
} } -Patrick Brownell
} } } ----------
} } } From: Katja M. Wolski[SMTP:kwolski-at-hsc.usf.edu]
} } } Reply To: kwolski-at-hsc.usf.edu
} } } Sent: Wednesday, January 03, 2001 4:26 AM
} } } To: Brownell, Patrick
} } } Subject: Re: Ralph Glass Knife problems
} } }
} } } I use a different knife machine, but what's the problem you're
having?
} } } Are you
} } } not getting a good knife edge or what?
} } }
} } } Katja
} } }
} } }
} } }
} } } "Brownell, Patrick" wrote:
} } }
} } } }
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
-----------------------------------------------------------------------.
} } } }
} } } } Hi all!
} } } }
} } } } I use Ralph type glass knives for plastic sectioning of biological
} } } samples.
} } } } I am experiencing problems in making a proper knife. I have a TAAB
} } } } histoknifemaker (also says Reichart-Jung). Is there anyone willing
to
} } } offer
} } } } expertise in this area?
} } } }
} } } } -Patrick Brownell
} } }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}





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From daemon Mon Jan 8 04:55:47 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 08 Jan 2001 03:07:35 -0800
Subject: digital media for EM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We are using Magneto-Optical Disks, 2.6 or 5.2 Gb to store the data. They
are very reliable (manufacturer claims, that data may be stored up to 50
years) it's not sensitive for magnetic fields and heat (in reasonable
temperature diapason, actually until melted). You may rewrite data many
times (a million, I believe). Because of reliability, US government uses
this media to store all digital data. MO disk needs special drive, which
suppose to be connected to the computer (to the PC in our case,
SCSII). It's connected to the network, so it is accessible from other
computers. We have to insert/remove MO disk by hands of coarse. 2.6 Gb MO
is about $40-80 each. MO drive - about $1000. I am not so happy with this
instrumentation (relatively slow, but faster than CD or Zip-drive, you have
to have special MO-drive connected to the particular computer etc), but
it's only known to me a media, which is reliable: CDR/CDRW - absolutely not
reliable, light-sensitive, etc.; Zip-drive/diskette is not enough for me,
HD- not bad idea, but technically not that easy (you have to have removable
HD, I did have a problem with that setup when WinNT changed the letters to
the logical drives and therefore all my programs suddenly stopped when I
removed the HD, and you have to restart the computer if you want to remove
HD). Currently, I do have approximately 10 Gb data stored on MO
disks. In our Department there are 200 or so disks are in use. To my
knowledge we did have one case when MO disk virtually lost the data but all
100% data has been recovered later. Something like that may happens if you
will try to remove the disk during the writhing (what, probably was
happens). If I am going to work with some block of data, I copied that to
the server and work on it from any computer even from home. The same
happens with fresh portion of data: I temporary store the data on the
server and then (after frequent reminding from SysAdmin), transfer it to
the MO disk. It takes approximately 20 min to transfer 1 Gb of
data. Detailed information about MO disks you may find on the Internet.

Sergey




CEPE} | {A

(310) 453-0748 (home)
(310) 825-1144 (office)
Pager: (310) 845-0248
mailto: sryazant-at-ucla.edu



From daemon Mon Jan 8 06:51:59 2001



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 08 Jan 2001 07:47:55 -0500
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To help improve the reliability of ZIP disk there is a freeware program
available from Gibson Research, TIP.exe . see http://www.grc.com


At 08:16 PM 1/5/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 8 07:20:30 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Mon, 8 Jan 2001 08:17:59 -0400
Subject: Re: Movie Trivia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} And there were all those Zeiss microscopes in Jurassic Park. Pity the one
} they were using in one scene (an Axiophot?) didn't have a condenser....
}
And let's not forget the Cambodian lady in "Blade Runner" (1982) who
operates an SEM in the street (in the rain!!?). She helps Harrison Ford
identify a scale in evidence as having come from a bioengineered snake, not
a fish (bioengineered animals apparently have serial numbers on even their
smallest parts).
There was another, more recent movie called "Mimic" in which there is a
scene where an insect specialist has a pile of SEM micrographs on her desk,
purportedly representing various insect eggs or larvae. As a former
micropaleontologist, I could tell they were actually illustrations of
planktonic Foraminifera, but I suspect this distinction was lost on much of
the viewing public.

Frank Thomas
Geological Survey of Canada
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Mon Jan 8 08:31:23 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 8 Jan 2001 07:14:07 -0800 (PST)
Subject: Re: Ultimate and perhaps last of TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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Jane,

I have done a lot of EM on ZnO and CaCO3 crystals a while ago. I simply made
a suspension of the powder in ethanol or methanol and ultrasonicated them
for 3 to 5 min. I then just dropped one or two drops of the suspension on
the grid positioned on a filter paper and let it dry. You might want to
check with the SEM that you do not change morphologies by the ultrasound
treatment. I suggest that you first take some SEM images anyway to get some
information on sizes, distributions and morphologies. IF you have rather
large crystals. SEM will not work, if the crystals are too small. In that
case you will have to go directly to the TEM. If you are taking the crystals
directly from a reaction solution, just give a drop or two on the grid and
let dry. Of course in that case, you might also have non CaCO3 material on
the grid depending on your reaction. You will have to make sure that you are
looking at the CaCO3 and not some other crap. This can be done by electron
diffraction or HRTEM. Image recording possibilities depend on your
microscope. What microscope are you using ? If you need further information
or want to discuss some things feel free to send an email.

Andreas

*************************************************
Dr. Andreas Taubert
Materials Science and Engineering Dept.
3231 Walnut Street
The University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************


----- Original Message -----
} From: {jwahom01-at-tufts.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, January 07, 2001 11:23 AM


Or not. I guess I just can't ignore this thread. I have always been vocal
about inconsistencies/stupidities/errors in TV/movies/etc. So, as to
Andromeda Strain: first of all, there was the insertion of the specimen
without use of an airlock (and those of us who suffered with the Forgflo/nee
RCA EMU-4 know that the beast had this horrid airlock that used the external
bellows minipump!!!); then there was the immediate location of the desired
area under the beam....; plus all those already mentioned. On Quincy: the
first episode (or maybe 2) the lab was fully outfitted with Zeiss equipment;
the rest of the series the lab was outfitted with AO (must be nice to have
that kind of equipment budget--but why go that direction??? could it be
politics???); then the SEM/microprobe episode, where, once again, the area
of interest with the decisive inclusion was magically right in the area
being examined immediately; then there was the TEM episode where Sam
received the biopsy about 4am and had a block, stained sections and a
confirmatory diagnosis by 8am (now there's a reality check for you--did your
boss pick up on that and demand that turn-around for you????); I'm sure
there were others but those stuck (mostly in my craw). And a final overall
plaint about the fact that everybody was working (at high mag, no less) in
brightly lit rooms, when we toiled away in near pitch dark!! Even that
Forgflo/RCA with its ma beam current couldn't do that--I know from many
hours in the dark, dark adjusting my eyes.

O well, as someone put it--that's Hollyweird.

Roger Moretz Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Sat, 06 Jan 2001 11:19:43 -0800, Robert Ruscica wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have enjoyed reading all the TV trivia regarding the Quincy and
Andromeda
} Strain. What many of you may not know is that for the "Strain" movie the
} makers of the film contacted a now defunct Microprobe Company, Materials
} Analysis Co. We sent to the studio an Electron Microprobe. The only
portion
} that made the film was some blinking x-ray scalers and a portion of the
} electronics rack. What the impact of microprobe was in the film I can't
} guess or remember.
} In the Quincy episodes, there were more than one, we sent to the studio
an
} ISI SEM along with a service engineer, whose name at the moment escapes
} me, to install it and our application and Demonstration man, Bill Roth
to
} run the SEM, Bill has been with Hitachi for many years now and could give

} more incite as to what happened at the studio than I can as he was there
} for a week doing the one episode. I remember him telling me that Quincy's

} technician, Sam was explaining to Quincy what was on the CRT with the
} camera going from the control panel with Bill's hands being filmed but
the
} overall shot of Sam and Quincy looking at the CRT and discussing the
} forensic material. I still have some 8x10's around here somewhere of the
} filming. Thought you might be interested.
}
} Regards,
} Bob Ruscica
}
}





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From daemon Mon Jan 8 10:03:51 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Mon, 8 Jan 2001 10:59:28 -0500
Subject: TEM help making a uranyl acetate stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I need some assistance in making up a uranyl acetate stain. I have powdered
uranyl acetate and would like to know what concentration and solvent to use
to make a good stain for looking at meat samples in the TEM. If there are
any procedures (time and temp) that I should adhere too that would also be
much appreciated.

Ps. I'm microtoming the TEM samples first then applying the uranyl acetate
stain with a follow-up stain of RuO4 vapor.

thanks all
dz

David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Mon Jan 8 14:11:20 2001



From: C Daniels :      cdaniels-at-gatan.com
Date: Mon, 8 Jan 2001 12:05:52 -0800
Subject: Job Opening - Analytical Product Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Analytical Product Manager - Gatan, Inc.

Gatan, Inc the technology leader in digital imaging, analytical spectroscopy
and ion beam milling applications for electron microscopy is seeking
candidates for an Analytical Product Manager. This person will manage the
complete product life cycle for this company's product line.
Responsibilities include coordinating all activities necessary to achieve
the strategic revenue and profit objectives for the product. For the
Product manager there are three key product cycle phases: 1) Planning:
includes market research, Marketing plan production and results in a vision
statement to pass to development. This the key stage in the development of
any new product. The vision statement is the marketing vision for the
product and it may include an analysis of competitor's products and a
projection of opportunities in the future. 2) Development: Pricing, product
positioning, product packaging and product promotion.3) Stabilization: Beta
testing, code testing and feedback to developers and product launch. The
applicant must have significant product management experience, preferably in
a product area that is applicable to market: TEM applications, electron
microscopy, biological, materials or semiconductor experience would be
useful. Familiarity with existing vendors, consultants, competitors, etc in
the industry is a plus. Technical knowledge of Gatan software and hardware
applications. A proven track record in both planning and executing
successful product management programs. Must be comfortable with current
development environments and development tools to work intelligently with
engineering. Must have excellent overall PC computing skills, including a
thorough familiarity with market tools in document composition, database
design and presentation management. MBA preferred; PHD desired with a
background in TEM and experience with GIF and PEELS systems. Salary: Base
salary plus bonus commensurate with experience.
Interested candidates should send email or fax their resume to:

GATAN, INC
Attn: HR Department
5933 Coronado Lane
Pleasanton, CA. 94588
Fax: (925) 463-0204
Email: hr-at-gatan.com
www.gatan.com



Carlotta Daniels
GATAN, Inc.
Human Resources
Pleasanton, CA. 94583





From daemon Mon Jan 8 14:38:36 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 8 Jan 2001 13:28:46 -0700
Subject: digital media for EM images

Contents Retrieved from Microscopy Listserver Archives
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OK, I think I'll add to the confusion ....

First of all, here are two URLs with what I found interesting data (and
opinions) about CD media.

http://thetechnozone.com/pcbuyersguide/hardware/storage/CD-R_reliability_rep
orts.html

http://www.ctnews3d.com/zzz/art35.html

there are also a number of follow-up links, which I have not explored.

The latter link has the following section:

So, How Long Can CDs Last?
Leaving aside scratches, fires, floods, and peanut butter sandwiches and
concentrating on the slow chemical changes that determine the inherent life
expectancy of a CD, extensive accelerated-aging tests suggest that Kodak
writable CD products, including Photo CD discs, will not reach a BLERmax of
50 for a period of around 200 years when kept in the dark at moderate
storage conditions. This long potential life expectancy is mainly a function
of the greater dark stability of the dye used in Kodak writable CD products.
Considering that BLERmax 50 is still not an unreadable level of error, Kodak
writable CDs have a very long life expectancy indeed. Similar research by
the 3M Company shows that CD-ROM products made by them will not attain a
block error rate of 50 per second for more than 100 years in moderate
storage conditions.

Accelerated aging is subject to uncertainties, but it does rest on firm
scientific footing. Behind the data is the simple assumption that raising
the temperature causes the reactions of decay to happen faster--so fast, in
fact, that they occur within a few months, rather than decades. The science
of reaction rates is called kinetics, and the lifetime predictions are based
on well-established principles of that branch of chemical science. These
same principles are used every day to design the chemical plants and
processes of the modern world. Because there is so much practical experience
with the laws of kinetics, lifetime predictions based on them are
approximately correct. Such test methods soon will be part of a forthcoming
ANSI (American National Standards Institute) standard dealing with tests for
CD permanence.

End of citation.

So, from a scientific standpoint (accelerated aging), a good writeable CD
should have a lifespan of 100-200 years, which is more than tape (several
decades) and on par with film, at least as far as I can tell. Film suffers
from the same problems the CD suffer from (sensitivity to light, scratches,
fire, alien attacks, etc.).

Finally a few words regarding M/O and CD:

We supplied with our systems until a few years ago an M/O drive. At that
time, there were only CD-ROMs. The drives were (are?) fairly expensive,
about $1000 for the drive, and with the advent of cheap CD-Rs it became
harder and harder to find drives and media. The drives are much more common
in Europe. In addition, the drives are pretty slow, and it was always a
hassle to work with the drives under Windows NT. The drives use a laser to
heat the storage medium, then a magnetic head to write the info to the
heated patch. This might result in a more stable storage than CD or
magnetic. The availability, however, seems to be a big problem.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Monday, January 08, 2001 4:08 AM
To: microscopy-at-sparc5.microscopy.com


Hello,

We are using Magneto-Optical Disks, 2.6 or 5.2 Gb to store the data. They
are very reliable (manufacturer claims, that data may be stored up to 50
years) it's not sensitive for magnetic fields and heat (in reasonable
temperature diapason, actually until melted). You may rewrite data many
times (a million, I believe). Because of reliability, US government uses
this media to store all digital data. MO disk needs special drive, which
suppose to be connected to the computer (to the PC in our case,
SCSII). It's connected to the network, so it is accessible from other
computers. We have to insert/remove MO disk by hands of coarse. 2.6 Gb MO
is about $40-80 each. MO drive - about $1000. I am not so happy with this
instrumentation (relatively slow, but faster than CD or Zip-drive, you have
to have special MO-drive connected to the particular computer etc), but
it's only known to me a media, which is reliable: CDR/CDRW - absolutely not
reliable, light-sensitive, etc.; Zip-drive/diskette is not enough for me,
HD- not bad idea, but technically not that easy (you have to have removable
HD, I did have a problem with that setup when WinNT changed the letters to
the logical drives and therefore all my programs suddenly stopped when I
removed the HD, and you have to restart the computer if you want to remove
HD). Currently, I do have approximately 10 Gb data stored on MO
disks. In our Department there are 200 or so disks are in use. To my
knowledge we did have one case when MO disk virtually lost the data but all
100% data has been recovered later. Something like that may happens if you
will try to remove the disk during the writhing (what, probably was
happens). If I am going to work with some block of data, I copied that to
the server and work on it from any computer even from home. The same
happens with fresh portion of data: I temporary store the data on the
server and then (after frequent reminding from SysAdmin), transfer it to
the MO disk. It takes approximately 20 min to transfer 1 Gb of
data. Detailed information about MO disks you may find on the Internet.

Sergey




CEPE} | {A

(310) 453-0748 (home)
(310) 825-1144 (office)
Pager: (310) 845-0248
mailto: sryazant-at-ucla.edu



From daemon Mon Jan 8 14:48:55 2001



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Mon, 08 Jan 2001 12:45:29 -0800
Subject: Re: Ralph Glass Knife problems

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Gordon Couger wrote:

}
} Could someone post the reference for the article for making glass knives
} by hand?

Here's the original reference, which includes a bit of the history of Ralph
knives (in honor of the late Dr. Paul Ralph):
Stain Technology 51(2): 71-97. [1976]. Bennet et al.
Science and art in preparing tissues embedded in plastic for
light microscopy,
with special reference to glycol methacrylate, glass knives and
simple stains.

Good luck.
Mike Nesson
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu




From daemon Mon Jan 8 15:12:08 2001



From: ERIC :      biology-at-ucla.edu
Date: Mon, 08 Jan 2001 13:11:31 -0800
Subject: Re: Computing for microscopy and imaging

Contents Retrieved from Microscopy Listserver Archives
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Out here in Sunny Southern California we just purchased a AMT 1K digital
camera for use in our EM lab at the UCLA Medical Center.
The storage device or choice for archiving we utilize is to burn CD's of
the images as long term storage.

===========
} } Just a word of caution about Zip disks. They can be very
} } unreliable. When Iomega first came out with them, their
} } media was really good. Now it is not the same. Maxell
} } and Fujifilm also make media. I think that theirs are better.
} }
} } The other problem with Iomega drives (Zip and Jaz) is the
} } click of death. This is a precursor to a dead drive or
} } media and loss of all that is on the media (if bad media).
} }
} } To check your drives and media, run tip.exe. To find out
} } more about this, visit http://www.grc.com
} }
} } gary g.
}
} Gary,
}
} Zip files not Zip disk. Use Pkzip or some other program to put all the
} files into one zip file, usually a complete directory, copy that file to
} the back up media and verify the zip file on the back up media. Currently
} I use write only CD-ROMs and keep a copy of really important stuff on my
} internet server at my ISP as well. Hard disk space is cheap. I have the
} last 15 years work at my finger tips. If I can find it:) I started using
} this on single sided single density floppies on a Radio Shack Model 1. I
} can only think of two times that I was unable to retrieve a file in 20
} years.
}
} I am a programmer not a photographer so a years work might fit on a floppy
} disk.
}
} I am not satisfied with the life of CD-ROMs but so far something better
} has always come along before the old media went bad and I copied it all
} over. One thing I don't like about CD-ROMs is they are not protected from
} scratching or being broken.
}
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}



From daemon Mon Jan 8 15:21:04 2001



From: ERIC :      biology-at-ucla.edu
Date: Mon, 08 Jan 2001 13:21:08 -0800
Subject: Archiving Digital Images

Contents Retrieved from Microscopy Listserver Archives
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On the HP CD Writer there are two programs for writing to a CD.. Out here
we use the CD-R one time write to the disc.

There is the HP program for writing a CD which does not have a way to
verify the CD was written. There is also another program that comes with
the CD writer from HP that performs a write speed test on the disc then
writes to the disc and then it verifies the disc... the program is called
MY CD on the HP CD writers....




From daemon Mon Jan 8 15:40:58 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 8 Jan 2001 11:37:48 -1000 (HST)
Subject: Temperture on TEM stage

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Happy New Year to you all!

How may I calculate (OK, make an educated guess) the temperture on my grid
in the TEM? I know it depends on the high voltage, the beam current, the
thickness of the specimen, the contact between grid and holder, and the
phase of the moon, and so probably I won't *really* know. All I need is a
ballpark figure, like is it 80C or 200C or 1500C?

I'm watching something presumably melt and fuse/crystallize on my LEO 912
EFTEM at 100-120 kV with a beam current of somewhere in the neighborhood
of 10 uA. This is not necessarily a bad thing - it tells me something
useful about these Si nanoparticles!

Mahalo,
Tina

Sunny, clear, about 74F, South Shore flat, North Shore up.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Jan 8 16:16:08 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 8 Jan 2001 17:11:35 -0500
Subject: Re: Temperture on TEM stage

Contents Retrieved from Microscopy Listserver Archives
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Happy New Year to you all!

How may I calculate (OK, make an educated guess) the temperture on my grid
in the TEM? I know it depends on the high voltage, the beam current, the
thickness of the specimen, the contact between grid and holder, and the
phase of the moon, and so probably I won't *really* know. All I need is a
ballpark figure, like is it 80C or 200C or 1500C?

I'm watching something presumably melt and fuse/crystallize on my LEO 912
EFTEM at 100-120 kV with a beam current of somewhere in the neighborhood
of 10 uA. This is not necessarily a bad thing - it tells me something
useful about these Si nanoparticles!

Dear Tina,
I posted something similar to the list a while ago, and wrote up a more
complete version for Microscopy Today. Although the variables are different,
the technique should be the same. A number you'd need would be the stopping
power of Si for 100 kV e-, which is 3.274 Mev cm^2/gm. The radiative stopping
power is much smaller than the collisional stopping power, which is 3.265 MeV
cm^2/gm (since the radiation should escape the particle, use this latter
number). Use this to calculate how much energy is transmitted to the specimen
for each electron by multiplying the stopping power by the density of Si (2.33
gm/cm^3) to get how much energy is deposited per unit path length, figuring out
the path length of each electron, and using the beam current flux (in e-/nm^2 or
the like) to get how many e- strike the particle each second. Next, calculate
the heat losses by conduction and radiation as a function of temperature, and
the steady-state temperature of the specimen--the number you want--will be where
the heat absorbed is equal to the heat lost. I assumed that heat radiated and
that conducted from the immediate vicinity of the particle would be lost in an
essentially infinite heat sink, and that the moon was in third quarter.

Sunny, clear, about 74F, South Shore flat, North Shore up.

Cloudy, about 270 K, Hudson icy.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us







From daemon Mon Jan 8 17:51:53 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Mon, 8 Jan 2001 17:46:55 -0600
Subject: Re: need help on X-EDS k factors

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Two minerals are fairly easy to come by Zircon (SiZrO4) and Hafnon
(SiHfO4) or could be synthesized. The rare-earth elements more
commonly form phosphate minerals such as monazite ((Light REE)PO4)
and xenotime ((Heavy REE,Y)PO4). The substitution of Huttonite
(SiThO4) into these structures would allow for the calculation of
REE,Y/Si k-factors. The problem with these minerals is that they are
normally not homogeneous, so special care must be made to correlate
electron microprobe with TEM analyses. Alternatively, you could try
to synthesize your own standards.
Hope this helps,
Ken
}
} Hi, everybody !
}
} I would greatly appreciate some hints concerning the kind of materials
} (minerals ?) that
} could be used in order to determine the k(A,B) (Cliff-Lorimer approx.)
} factors for my instrument
} (TEM CM-200 Philips microscope with EDAX EDS X-ray spectrometer) concerning
} Zr(K-lines),
} Y(K-lines), Hf(L-lines) and La(?-lines). Do they exist Si containing minerals
} which could be
} used to determine the k factors relating Si and the above mentioned elements ?
} Are they suppliers of standard materials that could be used for determining
} the k factors for the
} above mentioned elements ?
}
} Thank you in advance !
}
} Corneliu Sarbu, PhD
} Dept.of Metallurgy and Applied Materials Science
} Catholic University of Leuven
} Belgium




From daemon Mon Jan 8 17:53:32 2001



From: Pbgrover-at-aol.com
Date: Mon, 8 Jan 2001 17:49:33 -0600
Subject: one more TV trivial

Contents Retrieved from Microscopy Listserver Archives
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Around 20 years ago, I saw a movie about killer bats in which, after looking
at a slide with what looked like a Tasco microscope, the scientist exclaimed
"It's just as I feared - the rabies bacillus!". To add insult to injury there
followed a view through the microscope where, if I recall correctly, a
paramecium was swimming.

Paul




From daemon Mon Jan 8 17:53:39 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Mon, 8 Jan 2001 17:49:51 -0600
Subject: storage media

Contents Retrieved from Microscopy Listserver Archives
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Colleagues,

It's my understanding that dye-based CD-ROMs aren't archival, but the
gold-based version is. Anyone know more about this?

Dee



***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Mon Jan 8 18:58:18 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Mon, 8 Jan 2001 20:01:30 -0500
Subject: to Philip Oshel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

sorry for bothering you with that. I wanted to send an email to Phil
Oshel, but deleted the email before writing down his email address.
Phil, please contact me off-list, thanks.

Andreas

*************************************************
Dr. Andreas Taubert
Materials Science and Engineering Dept.
3231 Walnut Street
The University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************



From daemon Mon Jan 8 19:56:51 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 08 Jan 01 18:03:02 -0800
Subject: Re: Ultimate and perhaps last of TV Trivia

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: Re: Ultimate and perhaps last of TV Trivia
Dear All,

I too have been enjoying this conversation and thought I would sit back and read. However, I can't resist a few comments. After all we have one of the actual Zeiss microscopes from Jurassic Park in our lab. It works wonderfully, as a Zeiss microscope should. The only madification they made was to paint it pink to look right on film. Being so close to Hollywood, we have a few other pieces of famous furniture around the lab.
I must admit that I too look out for appearances of microscopes, and especially EM's in the media, but I think I must be a little more tolerant of the way we are portrayed. My phylosophy is that as long as we are out there, then it is a good thing. Who cares if the portrayal is accurate or not, we can sort that out in our lectures. At least we are being recognized. After all, it is much better to be talked about (no matter the subject) than to be ignored.

If we systematically went through film portrayals of any subject then I am sure we will find Hollywood had managed to insult just about every scientific disipline, ethnic group and foreign country. Who cares, its only entertainment. Watch out instead for the school science books that show cells with organelles but do not show the Golgi complex, or the histology books that omit the lysosomes. They are there. This is far more harmful to the scientific community.

I enjoyed the comments from Roger Moretz about the speed samples are processed. After all one of the roles of this form of entertainment has become a predictor of the future. Maybe the portrayals are so so inaccurate after all. We already work with microscopes in full daylight (from computer screens), we are close to having 4hr sample processing for resin-embedded samples (cf microwave processing), and we can already section and examine biopsies in less than 2 hr (with cryosectioning methods). Two thumbs up for Hollywood for showing us the way.

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Roger Moretz wrote:

} Or not. I guess I just can't ignore this thread. I have always been vocal
} about inconsistencies/stupidities/errors in TV/movies/etc. So, as to
} Andromeda Strain: first of all, there was the insertion of the specimen
} without use of an airlock (and those of us who suffered with the Forgflo/nee
} RCA EMU-4 know that the beast had this horrid airlock that used the external
} bellows minipump!!!); then there was the immediate location of the desired
} area under the beam....; plus all those already mentioned. On Quincy: the
} first episode (or maybe 2) the lab was fully outfitted with Zeiss equipment;
} the rest of the series the lab was outfitted with AO (must be nice to have
} that kind of equipment budget--but why go that direction??? could it be
} politics???); then the SEM/microprobe episode, where, once again, the area
} of interest with the decisive inclusion was magically right in the area
} being examined immediately; then there was the TEM episode where Sam
} received the biopsy about 4am and had a block, stained sections and a
} confirmatory diagnosis by 8am (now there's a reality check for you--did your
} boss pick up on that and demand that turn-around for you????); I'm sure
} there were others but those stuck (mostly in my craw). And a final overall
} plaint about the fact that everybody was working (at high mag, no less) in
} brightly lit rooms, when we toiled away in near pitch dark!! Even that
} Forgflo/RCA with its ma beam current couldn't do that--I know from many
} hours in the dark, dark adjusting my eyes.
}
} O well, as someone put it--that's Hollyweird.
}
} Roger Moretz Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals, Inc.
} On Sat, 06 Jan 2001 11:19:43 -0800, Robert Ruscica wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} } } } } } I have enjoyed reading all the TV trivia regarding the Quincy and
} Andromeda } } Strain. What many of you may not know is that for the "Strain" movie the } } makers of the film contacted a now defunct Microprobe Company, Materials } } Analysis Co. We sent to the studio an Electron Microprobe. The only
} portion } } that made the film was some blinking x-ray scalers and a portion of the } } electronics rack. What the impact of microprobe was in the film I can't } } guess or remember.
} } In the Quincy episodes, there were more than one, we sent to the studio
} an } } ISI SEM along with a service engineer, whose name at the moment escapes } } me, to install it and our application and Demonstration man, Bill Roth
} to } } run the SEM, Bill has been with Hitachi for many years now and could give
}
} } more incite as to what happened at the studio than I can as he was there } } for a week doing the one episode. I remember him telling me that Quincy's
}
} } technician, Sam was explaining to Quincy what was on the CRT with the } } camera going from the control panel with Bill's hands being filmed but
} the } } overall shot of Sam and Quincy looking at the CRT and discussing the } } forensic material. I still have some 8x10's around here somewhere of the } } filming. Thought you might be interested.
} } } } Regards,
} } Bob Ruscica
} } } }
}
}
}
}
}
} _______________________________________________________
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} Subject: Re: Ultimate and perhaps last of TV Trivia
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From daemon Mon Jan 8 21:28:14 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 08 Jan 2001 19:22:56 -0800
Subject: Re: storage media

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They are all dye-based. What is silver or gold is the coating
on the top of the media. It ca readily be flaked off.

With the metal coating removed, the media is generally
transparent. Some have a blue tint while others have
little color. Others might look green. But essentially,
they are all chalgocencide technology. Some are
better than others.

gg


At 03:49 PM 1/8/01, you wrote:

} Colleagues,
}
} It's my understanding that dye-based CD-ROMs aren't archival, but the
} gold-based version is. Anyone know more about this?
}
} Dee
}
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 914/365-8640
} F: 914/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)



From daemon Mon Jan 8 21:30:04 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 08 Jan 2001 19:26:38 -0800
Subject: Re: one more TV trivial

Contents Retrieved from Microscopy Listserver Archives
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These are found all of the time in Hollywood. They are stand-ins.
Only a very sharp eye can tell the difference. Notice how well
Antonio Banderas dances in "The Mask of Zorro?" Real,
or a stand-in? duh.

gary



At 03:49 PM 1/8/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jan 9 03:45:24 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Tue, 09 Jan 2001 09:39:37 +0000 (GMT)
Subject: Re: Temperture on TEM stage

Contents Retrieved from Microscopy Listserver Archives
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Hi Tina,
I'm sure this question has been asked before, (look in the archives) - but I think the basic answer is 'it depends'(!). I am currently having terrible trouble with plucked FIB sections of Au metallised GaAs on holey carbon films; the metal melts, alloys with and destroys my specimen even at relatively low beam currents in my JEOL 120 CX. I have been told by our theory guys that little heat will escape by radiation below about 500C, so if you have a sample with very poor thermal conductivity it will be easy to get to this temperature. You may be able to avoid the problem somewhat by using higher accelerating voltages (less inelastic interaction with the specimen, I believe) and/or adding a carbon coating to the specimen to increase thermal conductivity.

Good luck!

Richard

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Happy New Year to you all!
}
} How may I calculate (OK, make an educated guess) the temperture on my grid
} in the TEM? I know it depends on the high voltage, the beam current, the
} thickness of the specimen, the contact between grid and holder, and the
} phase of the moon, and so probably I won't *really* know. All I need is a
} ballpark figure, like is it 80C or 200C or 1500C?
}
} I'm watching something presumably melt and fuse/crystallize on my LEO 912
} EFTEM at 100-120 kV with a beam current of somewhere in the neighborhood
} of 10 uA. This is not necessarily a bad thing - it tells me something
} useful about these Si nanoparticles!
}
} Mahalo,
} Tina
}
} Sunny, clear, about 74F, South Shore flat, North Shore up.
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
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From daemon Tue Jan 9 04:30:15 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 09 Jan 2001 10:39:24 +0000
Subject: Re: Archiving Digital Images

Contents Retrieved from Microscopy Listserver Archives
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Dear all

It's a bit late but may I wish you a happy new year.

My personal experience is that the biggest problem with CD writing is the
possibility of corrupting the disk and not noticing (e.g. buffer under runs).
This can at the least result in wasted time and frustration and at worst the
actual loss of data if not properly backed up elsewhere. I haven't seen this
mentioned so I thought I would bring up the subject of 'Burn proof' CD writers
which have appeared in the last 6-12 months. They apparently have built in
hardware/software which should mean that you can now use CDs as super floppies
and not have to worry about turning off conflicting software before writing
large chunks of data to CD. The magazine reviews have raved about this stating
that you could play 'Quake' or access the internet and still write to a CD
without error. Has anyone had any experience of these new drives?

Archival quality of CDs, ZIPs and magneto opticals has been discussed many
times before and the usual conclusion is that the technology will be defunct
before good quality discs, stored properly will be significantly corrupted. My
money has to stay with CDs as a cheap, reliable and
'likely-to-be-around-in-a-decade' technology (e.g. all modern DVD drives still
read CDs). CD/DVD technologies still have a long way to go in terms of data
density and so multi-format reading machines would seem more likely than with
magnetic media ... unless you know better. I'm sure that I read of recent
developments in dyes/pigments (maybe it was Kodak - I don't know) that should
greatly reduce writeable CDs deterioration in light. One thing's for certain
if I want to distribute images to students, researchers or other users there
is only one universal technology (some new PCs don't even have a floppy drive
now).

Another thread was discussing e.m. film scanners and I think I should mention
the Epson Perfection 1200P which is a USB scanner that can be used with A4
prints or 5x4 inch negatives. It has a true resolution of 1200dpi and an
optical density range of 3.2 and costs a little over 200 UK pounds. Much of
our work with undergraduates and researchers has been done at 600dpi (which
should easily allow an equivalent of 3x print enlargement) and dare-I-say-it
JPEG formats (set at lowest compression to retain most detail). The film is,
after all, our archive and the digital images are at present for cataloguing,
rapid retrieval and project write ups - the students love it if only because
they don't spend hours in the darkroom. I am not suggesting that this
particular scanner will suit all users because it has a narrower OD range than
some, but is an excellent low cost way of trying the technology out while it
is still maturing. The biggest problem is that there was no film holder for
our format but cardboard and sticky tape have solved that problem,
temporarily. Also if you opt for the USB version then you really need Windows
98 on a PC (95 - including the the second version, NT etc. may all have
problems).

I have no commercial interest in any of the above technologies unless they
make my work or research any easier - in a year or two I may be in a better
position to judge that, objectively.

Malcolm Haswell
e.m. unit
University of Sunderland
UK

ERIC wrote:

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}
} On the HP CD Writer there are two programs for writing to a CD.. Out here
} we use the CD-R one time write to the disc.
}
} There is the HP program for writing a CD which does not have a way to
} verify the CD was written. There is also another program that comes with
} the CD writer from HP that performs a write speed test on the disc then
} writes to the disc and then it verifies the disc... the program is called
} MY CD on the HP CD writers....



From daemon Tue Jan 9 06:31:47 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 09 Jan 2001 12:39:16 +0000
Subject: Re: one more TV trivial

Contents Retrieved from Microscopy Listserver Archives
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Paul

I agree, but I don't blame the film makers. They are part of the majority of the
public who (sometimes proudly) proclaim to have no/little knowledge of science.
It seems to be quite common to use bugs, germs, viruses, bacilli and bacteria as
interchangeable terms. This can be extremely misleading when a national news
reporter warns of the latest outbreak of the meningitis 'virus' when he almost
certainly meant the bacterial form.

There has been more than one occasion where I have seen a TEM instrument with a
superb SEM image portrayed on film or TV (yes I know about TEM/SEM/STEMs - but it
wasn't) presumably because the TEM looks impressive and so do SEM pictures. I
also saw the Bladerunner SEM and another technology it had was a voice activated
TV with high resolution scanner and printer. It seemed well out of reach of the
average household at the time and although I haven't seen this on a TV this month
all of that technology is common on your average home PC now - maybe the next
Intel (QX4) microscope will be a toy SEM connected to your TV.

Malcolm Haswell
e.m. unit
University of Sunderland
UK

"Pbgrover-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Around 20 years ago, I saw a movie about killer bats in which, after looking
} at a slide with what looked like a Tasco microscope, the scientist exclaimed
} "It's just as I feared - the rabies bacillus!". To add insult to injury there
} followed a view through the microscope where, if I recall correctly, a
} paramecium was swimming.
}
} Paul



From daemon Tue Jan 9 08:26:22 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 09 Jan 2001 08:18:03 -0600
Subject: Re: storage media

Contents Retrieved from Microscopy Listserver Archives
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At 07:22 PM 1/8/01 -0800, Gary Gaugler wrote:
} They are all dye-based. What is silver or gold is the coating
} on the top of the media. It ca readily be flaked off.

No, supposedly the gold ones are actually made of gold
the metal, and the "silver" ones are aluminum. The Al will
oxidize over time if scratched, the Au won't.

The story says that if you scratch a disc deep enough to
expose the metal, it could degrade the data over time.
I don't put a lot of stock in this worry, though. After
all, it wouldn't take much more of a scratch to wipe out
a section of gold, too, and there's a big difference in
damage between a radial and a concentric scratch on any
media.

- John



From daemon Tue Jan 9 08:37:58 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 9 Jan 2001 06:34:14 -0800 (PST)
Subject: Re: Ralph Glass Knife problems

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Mike:

Thanks for coming up with the reference. I just KNEW it was it my Reference
Manager database (NOT). So, for several days now I have been trying to
figure out if I ever referenced it in a paper, or in notes or
methods/techniques/procedures.... Now I can sleep at night again :)

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Mon, 08 Jan 2001 12:45:29 -0800, Michael Nesson wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
}
} Gordon Couger wrote:
}
} }
} } Could someone post the reference for the article for making glass
knives
} } by hand?
}
} Here's the original reference, which includes a bit of the history of
Ralph
} knives (in honor of the late Dr. Paul Ralph):
} Stain Technology 51(2): 71-97. [1976]. Bennet et al.
} Science and art in preparing tissues embedded in plastic for
} light microscopy,
} with special reference to glycol methacrylate, glass knives and
} simple stains.
}
} Good luck.
} Mike Nesson
} _______________________________________________________________________
} Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
} 2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
} (541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu
}
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue Jan 9 08:58:05 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 9 Jan 2001 09:51:38 -0800
Subject: RE: EM - Negative Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our customers have enjoyed success with the Agfa "Duoscan" line of
scanners.
Fitting in your price range would be the Duoscan Hi-D. The Hi-D has a Dmax
of
3.8 (hence the name Hi-D), 1000x2000 hardware resolution, apochromatic
optics,
excellent software for both Mac and Windows, color management software, and
a SCSI interface. The Hi-D sells for under $2600.
Coming soon will be the Arcus 1200, which also has glassless film scanning,
1200x2400 hardware resolution with a SCSI interface. The Arcus 1200 will be
approximately $800-850 when available.
The Agfa T2500 is a similar design, with 2500x2500 hardware resolution and
3.4 Dmax. The T2500 does not fall in this price range, selling for under
$4300.
It is a phenomenal scanner for TEM. There is currently a $350 rebate on the
T2500.
All of these scanners share the Agfa Fotolook sofware driver for Mac and
Windows.
Unlike most "flatbed" scanners which place films on a glass bed for
scanning,
the Duoscan line utilizes a glassless holder similar to a negative carrier
in an
enlarger. There is no glass between the film and the CCD. This eliminates
Newton
Rings and other issues associated with scanning through glass.

Microtek International shares some hardware specs with the Agfa Duoscan
line
in their Artixscan series. The Artixscan 1100 would be the "sister" model
to the
Duoscan Hi-D with 1000x2000 optical resolution, 3.9 Dmax, SCSI
interface,etc.
Price is under $1750.
The Artixscan 2500 is similar to the Duoscan T2500- 2500x2500 optical res,
SCSI interface, etc. Price under $4000.

See Microtek at http://www.microtekusa.com/list.zhtml?cid=2
Agfa DuoScan HI-D at
http://www.agfahome.com/product/CatProd_DisplayPublic.html?id=4290
Agfa DuoScan T2500 at
http://www.agfahome.com/product/CatProd_DisplayPublic.html?id=3115
Agfa Arcus 1200 at
http://www.agfahome.com/product/CatProd_DisplayPublic.html?id=6130

I will be happy to provide literature, information or answer any questions.
Please contact me directly by phone or email.

Sincerely,

George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com




From daemon Tue Jan 9 10:34:49 2001



From: Jonathan Wilson :      wilson_jm-at-cimar.org
Date: Tue, 9 Jan 2001 16:30:50 -0800
Subject: cryostat tissue mounting stubs

Contents Retrieved from Microscopy Listserver Archives
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Hello, I was wondering if any one might know where I could get some cryostat
tissue mounting stubs. I am looking for the 'T' type looking stubs that fit
into a ball joint so the stub position can be adjusted in the cryostat
chuck. The ones I have used were made of copper and the pin diameter was1/8"
with a locking screw in the shaft of the ball joint.
Thanks for any leads.
Sincerely,
Jonathan

Jonathan Wilson (Ph.D.)
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR)
Universidade do Porto
Rua do Campo Alegre 823
4150-180 Porto, Portugal
tel 351 22 606 0421
fax351 22 606 0423
e-mail: wilson_jm-at-cimar.org
mop01258-at-mail.telepac.pt



From daemon Tue Jan 9 12:19:30 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 09 Jan 2001 13:13:00 -0500
Subject: Re: Archiving Digital Images

Contents Retrieved from Microscopy Listserver Archives
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Many years ago (at least 6!) when we had an early Pinnacle Micro CD writer
I got into the habit of checking my CD's by copying them back to the hard
drive. If they would copy without generating an error then they were OK (an
even better way was to do the copy on another CD drive). One fairly often
found one that was bad.

Today, using a more modern drive and faster computer I still do the same
thing (my software doesn't have a verify option), but I rarely find a bad
disk. However, it doesn't take much of my time, and I always think better
safe than sorry when it comes to an archive!

Tony.



* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Tue Jan 9 12:28:21 2001



From: ERIC :      biology-at-ucla.edu
Date: Tue, 09 Jan 2001 10:28:07 -0800
Subject: RMC ultramicrotome Parts needed?

Contents Retrieved from Microscopy Listserver Archives
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Microscopy List,

I need to get some parts for a RMC MX-600XL ultramicrotome.. I know RMC
was sold to another company from Ventana.. How do I or who do I contact
about some parts??


Eric A. Rosen
UCLA Medical Center
Dept. Pathology and Lab Medicine
Los Angeles, CA 90095





From daemon Tue Jan 9 12:46:57 2001



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 9 Jan 2001 10:43:39 -0800
Subject: 4by5 film scanners

Contents Retrieved from Microscopy Listserver Archives
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In the context of recent queries, I just thought I'd
post Nikon's most recent announcement ... see:
http://www.klt.co.jp/Nikon/Press_Release/ls-8000.html

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jan 9 12:48:26 2001



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Tue, 9 Jan 2001 10:45:33 -0800
Subject: 4by5 film scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the context of recent queries, I just thought I'd
post Nikon's most recent announcement ... see:
http://www.klt.co.jp/Nikon/Press_Release/ls-8000.html

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jan 9 12:59:51 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 9 Jan 2001 13:31:08 -0600
Subject: Re: 4by5 film scanners

Contents Retrieved from Microscopy Listserver Archives
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If you check the CD-FAQ:
http://www.landfield.com/faqs/cdrom/cd-recordable/part1/



This looked pretty interesting. Have you heard a ballpark price?


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--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Jan 9 14:10:21 2001



From: RCHIOVETTI-at-aol.com
Date: Tue, 9 Jan 2001 15:06:54 EST
Subject: Re: RMC ultramicrotome Parts needed?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 01/09/2001 11:30:40 AM US Mountain Standard Time,
biology-at-ucla.edu writes:

{ { I need to get some parts for a RMC MX-600XL ultramicrotome.. I know RMC
was sold to another company from Ventana.. How do I or who do I contact
about some parts??


Eric A. Rosen
UCLA Medical Center
Dept. Pathology and Lab Medicine
Los Angeles, CA 90095


} }

Eric,

RMC is now a part of Boeckler Instruments in Tucson, Arizona. You can reach
them at:

Boeckler Instruments, Inc.
4650 South Butterfield Drive
Tucson, AZ 85714
Tel. 800-552-2262
Fax 520-745-0004

Bob Chiovetti
GTI Microsystems


From daemon Tue Jan 9 16:11:14 2001



From: Earl Weltmer :      eweltmer-at-home.com
Date: Tue, 9 Jan 2001 14:07:46 -0800
Subject: EDS Detector problem

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have a Kevex EDS detector that was shipped via FEDEX with liquid nitrogen.
FEDEX, in their infinite wisdom, managed to tilt the detector and drain the
LN2 from the dewar.
(Although the packing box and "tilt watch indicators" say "do not tilt',
"this side up", etc.)

Upon inspecting the detector I have found that a plug on the side of the
dewar has been removed and the detector is at atmosphere. I suspect that the
LN2 froze the plug & "O" rings therby allowing air into the detector.

I am considering machining an adapter flange and repumping the detector.
This is, of course, assuming that the detector window is entact.

Does anyone have experience with this? While pumping, I am considering
pouring hot water into the dewar to outgass the system but I don't know if
this is a good idea.

Thank You,

Earl Weltmer



From daemon Tue Jan 9 17:12:24 2001



From: Gwyneth Hill Beagley :      beagleyg-at-alma.edu
Date: Tue, 09 Jan 2001 18:05:18 +0800
Subject: looking for TEM parts - again

Contents Retrieved from Microscopy Listserver Archives
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Hi -
I am looking for a used lens regulator circuit board for a Philips TEM
201. The parts # is Lens Regulator 5322 695 14022 in the 1974 service
manual -also called RB-U13. Is anyone junking a 201? The price from
FEI/Philips is a bit beyond my budget! g





From daemon Tue Jan 9 17:50:06 2001



From: Gwyneth Hill Beagley :      beagleyg-at-alma.edu
Date: Tue, 9 Jan 2001 17:45:37 -0600
Subject: looking for lens regulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi -
I am looking for a used lens regulator circuit board for a Philips TEM
201. The parts # is Lens Regulator 5322 695 14022 in the 1974 service
manual -also called RB-U13. Is anyone junking a 201? g




From daemon Tue Jan 9 17:51:42 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Tue, 9 Jan 2001 17:47:53 -0600
Subject: TEM: Energy Filtered Diffraction patterns

Contents Retrieved from Microscopy Listserver Archives
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Colleagues,
Has anyone had any experience with generating energy filtered
diffraction patterns (not CBED) with a Gatan GIF on a Philips CM? I
would like some pointers on intensity issues and summation of images.
Thanks in advance.
Ciao for now,
Ken




From daemon Tue Jan 9 17:52:12 2001



From: jcoleman-at-rdg.boehringer-ingelheim.com
Date: Tue, 9 Jan 2001 17:48:24 -0600
Subject: JELCO JSM 6700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are installing a new JEOLCO JSM 6700F Field Emission Scanning Electron
Microscope. This is a new model and incorporates some novel technology. We
are interested in communicating with others who are or will be working with
this model. Please respond off-line and I will construct a mailing list to
distribute questions, concerns and, of course, suggestions for operation and
improvement.

James R. Coleman, Ph.D.
Supervisor, Electron Microscopy Laboratories
Boehringer-Ingelheim Pharmaceuticals Inc.




From daemon Tue Jan 9 19:14:49 2001



From: Douglas Keene :      DRK-at-shcc.org
Date: Tue, 09 Jan 2001 17:07:30 -0800 (Pacific Standard Time)
Subject: Re: Nikon coolpix at low light levels

Contents Retrieved from Microscopy Listserver Archives
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Richard asked if the exposure meter on the Nikion coolpix
900 digital camera was sensitive enough to control exposure
in low light (immunofluorescent) conditions. We used the
camera to take images of samples labeled with moderate
intensely with FITC. We captured nice images in the "spot"
automatic metering mode. With the camera mounted in one
ocular, the camera chose to expose at F 3.1 and kept the
shutter open one second. However, it was a bit difficult
to use since the image did not appear on the display during
focusing. There was not enough light for real time
imaging. To insure a focused image, we needed focus in
brightfield illumination, then go to EPI to collect the
image. You could also use a monitor, but that would add
significant cost to the system.

It may be useful to realize that the camera has the ability
to be used in several automatic exposure settings
(programmed, aperture priority, shutter priority) (wide
field, narrow field, center spot) and in manual mode, where
you can use a timed exposure from 1/1000 to 8 seconds, or
longer (up to 60 seconds) by pushing the exposure once to
open and again to close.

Feel free to phone with more questions,

Doug

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org






From daemon Tue Jan 9 22:21:08 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 09 Jan 2001 22:37:18 -0500
Subject: Re: EDS Detector problem

Contents Retrieved from Microscopy Listserver Archives
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Earl-

It all depends. If there is no-one else who would pay to fix your
detector, then you have nothing to lose by doing what you suggest (incl.
the hot water). I do it relatively often, (but only to detectors that are
seriously degraded or not functioning at all), and it works, but I've never
had a crystal return to tip-top premium performance after this treatment.
(In fact, only today I deliberately vented a detector and re-pumped it, but
that is a long, and different, story). BTW, you only need to achieve a
quite modest vacuum during the pump - the molecular sieve will take care of
a lot of gas when it cools! Having said that, I have usually rigged up a
piece of vacuum hose from the pumping adapter to a port I had added to an
old evaporater, and pumped down to the 10^-5 - 10^-4 range. I have a
pumping adapter with a US thread for the plug - perhaps it will work on
your Kevex detector (in fact, it came from Kevex 20 years ago). If you
want to borrow it, let me have your FedEx or other shipping account number,
and shipping details, and I'll send it off.

On the other hand, if the shipper, or FEDEX, can be persuaded to pay to
have the detector refurbished, it will probably work better in the end, and
a do-it-yourself attempt beforehand may make your claim more difficult!

Good luck!

Tony

Tony.


At 02:07 PM 1/9/2001 -0800, you wrote:
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From daemon Wed Jan 10 03:08:40 2001



From: Kun Li :      k-li-at-mailcityasia.com
Date: Wed, 10 Jan 2001 16:57:09 +0800
Subject: staining chemical for low k HSQ

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Dear Listers,

Does any of you know the staining chemicals for low k HSQ so that HSQ and other kinds of oxides such as SOG and PETEOS can be delineated?

K. Li


Get your FREE Email at http://www.mailcityasia.com


From daemon Wed Jan 10 04:45:54 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 10 Jan 2001 02:43:54 -0800
Subject: Re: Temperture on TEM stage

Contents Retrieved from Microscopy Listserver Archives
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I suppose, if plastic film or plastic sections (biological samples) are
relatively stable under the beam, it may mean (may not) that temperature is
not so high. In my hands, overheating of the plastic film in air over
+150oC will destroy the film. I assume, something like that would happens
in the microscope if the beam generate such amount of heat (plastic film
itself has a low thermal conductivity, I believe). Another scenario:
unelastic scattered electrons may transfer kinetic energy (not necessary it
will immediately transferred into the heat) to the sample/film. This
excess of energy may heat the sample but may force molecules/atoms to
migrate for instance. Heavy atoms should affected more. I do remember, 20
or so years ago people shout films under TEM, how Pt or Au atoms may move
under the beam. They (atoms) tend to form big clusters under the
beam. This is explanation (to me), why the resolution of Pt or Au shadowed
samples is so bad. Pt-C shadowing succeeded because carbon protect Pt from
migration and forming big clusters. In general, I do agree: "it depends"...

Sergey

At 09:39 AM 1/9/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jan 10 05:11:29 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 10 Jan 2001 21:07:59 +1000
Subject: RE: EDS Detector problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did a few detector treatments and I agree with Tony's comments.
One addition: Obtain a fairly good vacuum before pouring boiling water (couple
of liters) into the dewar. The heat causes a dramatic drop in vacuum due to
outgasing of the molecular sieve. I would terminate pumping a couple of hours
after the hot water was added. Obviously is nice to have a clean vacuum system
available as backstreaming oil vapour would rather defeat the purpose.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, January 10, 2001 1:37 PM, Tony Garratt-Reed [SMTP:tonygr-at-mit.edu]
wrote:
}
}
} Earl-
}
} It all depends. If there is no-one else who would pay to fix your
} detector, then you have nothing to lose by doing what you suggest (incl.
} the hot water). I do it relatively often, (but only to detectors that are
} seriously degraded or not functioning at all), and it works, but I've never
} had a crystal return to tip-top premium performance after this treatment.
} (In fact, only today I deliberately vented a detector and re-pumped it, but
} that is a long, and different, story). BTW, you only need to achieve a
} quite modest vacuum during the pump - the molecular sieve will take care of
} a lot of gas when it cools! Having said that, I have usually rigged up a
} piece of vacuum hose from the pumping adapter to a port I had added to an
} old evaporater, and pumped down to the 10^-5 - 10^-4 range. I have a
} pumping adapter with a US thread for the plug - perhaps it will work on
} your Kevex detector (in fact, it came from Kevex 20 years ago). If you
} want to borrow it, let me have your FedEx or other shipping account number,
} and shipping details, and I'll send it off.
}
} On the other hand, if the shipper, or FEDEX, can be persuaded to pay to
} have the detector refurbished, it will probably work better in the end, and
} a do-it-yourself attempt beforehand may make your claim more difficult!
}
} Good luck!
}
} Tony
}
} Tony.
}
}
} At 02:07 PM 1/9/2001 -0800, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi all,
} }
} } I have a Kevex EDS detector that was shipped via FEDEX with liquid nitrogen.
} } FEDEX, in their infinite wisdom, managed to tilt the detector and drain the
} } LN2 from the dewar.
} } (Although the packing box and "tilt watch indicators" say "do not tilt',
} } "this side up", etc.)
} }
} } Upon inspecting the detector I have found that a plug on the side of the
} } dewar has been removed and the detector is at atmosphere. I suspect that the
} } LN2 froze the plug & "O" rings therby allowing air into the detector.
} }
} } I am considering machining an adapter flange and repumping the detector.
} } This is, of course, assuming that the detector window is entact.
} }
} } Does anyone have experience with this? While pumping, I am considering
} } pouring hot water into the dewar to outgass the system but I don't know if
} } this is a good idea.
} }
} } Thank You,
} }
} } Earl Weltmer
} }
} ***********************************************
} Anthony J. Garratt-Reed, M.A., D.Phil.
} Principal Research Scientist
} Room 13-1027
} Massachusetts Institute of Technology
} 77 Massachusetts Avenue
} Cambridge, MA 02137-4307
}
} Tel: (617) 253-4622
} Fax: (617) 258-6478
} ***********************************************



From daemon Wed Jan 10 06:16:01 2001



From: Alan Bright :      bright-at-dial.pipex.com
Date: Wed, 10 Jan 2001 12:09:06 -0000
Subject: cryostat tissue mounting stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jonathan,

If you have a problem locating the original suppliers of these object
holders, I should be able to make them for you, please fax me a sketch will
measurement and I will reply with a price.


Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com


-----Original Message-----
} From: Jonathan Wilson [mailto:wilson_jm-at-cimar.org]
Sent: 10 January 2001 00:31
To: Microscopy


Hello, I was wondering if any one might know where I could get some cryostat
tissue mounting stubs. I am looking for the 'T' type looking stubs that fit
into a ball joint so the stub position can be adjusted in the cryostat
chuck. The ones I have used were made of copper and the pin diameter was1/8"
with a locking screw in the shaft of the ball joint.
Thanks for any leads.
Sincerely,
Jonathan

Jonathan Wilson (Ph.D.)
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR)
Universidade do Porto
Rua do Campo Alegre 823
4150-180 Porto, Portugal
tel 351 22 606 0421
fax351 22 606 0423
e-mail: wilson_jm-at-cimar.org
mop01258-at-mail.telepac.pt




From daemon Wed Jan 10 07:08:19 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 10 Jan 2001 07:00:04 -0600
Subject: RE: EDS Detector problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Earl,

For about 15 years, I ran a Kevex "extra" detector. This was a windowless
type and over time (I did a lot of light element work) the crystal would ice
over. The Etec was turbo pumped, but between specimen out gassing and the
methane from the WDS, icing over time could not be avoided. I warmed and
pumped a number of times using warm/hot water. A small amount of
degradation occurred, but it did help overall. I later went to a hot air
gun and a tube to direct the air to the bottom of the dewar. By doing this,
I did not have to worry about drying the dewar.

Woody


From daemon Wed Jan 10 08:01:52 2001



From: Brian Gere :      vm89xt-at-zipee.net
Date: Wed, 10 Jan 2001 07:51:30 -0500
Subject: All of us #1ABA

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Jan 10 08:11:52 2001



From: Tom McKee :      tmckee-at-scilabs.com
Date: Wed, 10 Jan 2001 09:10:08 -0500
Subject: EM - used Denton DV 515 Evaporator

Contents Retrieved from Microscopy Listserver Archives
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Several folks have asked about chillers and evaporators lately.

We have a Denton DV-515 vacuum evaporator which has been replaced by an
automated DV-502A. The DV-515 was originally from a government lab so it
has been well maintained and cared for. We had used it mainly as a back up
for our DV-502A. We use the carbon rod arc to produce TEM support films and
general carbon coating of samples on MCE & PC filters during TEM specimen
prep and to put carbon coatings on SEM samples.

If you are interested, we would like 2500.
reply to:

tmckee-at-scilabs.com




From daemon Wed Jan 10 08:14:59 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Wed, 10 Jan 2001 09:08:51 -0800
Subject: RE: 4by5 film scanners

Contents Retrieved from Microscopy Listserver Archives
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All,
The preliminary information I have is that the Coolscan 8000 ED
will sell for approximately $4000 US. We will carry it when it becomes
available, currently listed as June. I hope to see it in early February.
Quick specs..
4000dpi optical resolution
IEEE1394 "Firewire" Interface- not SCSI
4.2 dynamic range 48bit images
Multi Sample Scanning

Info is also at
http://64.77.39.20/usa_product/product.jsp?cat=7&grp=703&productNr=9246

I'll pass along more info as it becomes available.

George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com
}
} This looked pretty interesting. Have you heard a ballpark price?
}
} }
} } In the context of recent queries, I just thought I'd
} } post Nikon's most recent announcement ... see:
} } http://www.klt.co.jp/Nikon/Press_Release/ls-8000.html
} }

}



From daemon Wed Jan 10 08:47:14 2001



From: Scott Robinson :      sjrobin-at-itg.uiuc.edu
Date: Wed, 10 Jan 2001 08:51:14 -0600
Subject: TEM/SEM: Coolscan 8000 ED film scanner

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I called Nikon yesterday (1/9/2001) to ask about the Coolscan 8000 ED. They
told me it would cost $3000 (U.S.) and is scheduled to be released in April.

Scott Robinson





From daemon Wed Jan 10 09:23:03 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 10 Jan 2001 10:02:01 -0500
Subject: Re: Ask-A-Microscopist:TEM of calcium carbonate crystals

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Question: I need to view calcium carbonate crystals with a transmission
electron microscope for a research project. I also need to record the
images. All I've managed to do so far is order thin carbon grids. I have
been asked to write a plan for my experiment but I have no clue how to
prepare crystals in solution for magnification or what tools to use.
Please give me some direction. My professors say I should figure
everything out myself - "It will be a good learning experience."

---------------------------------------------------------------------------

Dear Jane,
The most difficult aspect of this is likely to be getting sufficiently
small crystals dispersed over the grid surface so that you can see the
individual crystals. You do not say whether you need detail within the crystals
or whether just seeing silhouettes is sufficient. If the latter, then just
evaporate the solvent rapidly from a dilute CaCO3 solution placed on the grid.
This should leave small crystals dispersed over the grid. If you can't
evaporate on the grid, you will have to prepare the small crystals and transfer
them either by suspending them in a volatile liquid which doesn't dissolve CaCO3
or by wafting them into the air and collecting them on the grid. If you need
detail within the crystals, they must be very thin--no more than ~10 nm
(depending on the EM voltage), so you will have to find a way to get them this
thin, and, if possible, much wider than they are thin--a platy habit. Good
luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us







From daemon Wed Jan 10 09:23:05 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 10 Jan 2001 10:01:01 -0500
Subject: Re: Ask-A-Microscopist: LM: Crystal Growth

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------

Email: us004118-at-mindspring.com
Name: Leonard Lessin, FBPA

School: (Retired Science & Medical Photographer)

State: NY

Zip: 10012

Question: I am enjoying doing photomicrographs of crystal
preperations in polarized light.However I have insuffient knowledge of
chemistry
to choose solvents without a long series of trial and error efforts.Can you
give me
a rationale and/or a reference to go about this in a more productive
manner?

---------------------------------------------------------------------------

Dear Leonard,
There are several requirements for a suitable solvent: 1) it must, of
course, disolve the material you want to examine, 2) it must evaporate to
produce crystals in a habit suitable for your work (not too small, transparent,
etc.), and 3) it should not be too toxic even though you may have a fume hood
available. The key to 1) is "like disolves like". If you have an ionic salt or
a polar solute such as sucrose, water would be a good choice, if you have an
aromatic compound, such as naphthalene, an aromatic would work (I hesitate to
reccommend benzene, even though it gives nice crystals, because of its toxicity;
I would try toluene.), and if you have a lipid, like cholesterol, I'd use a
non-polar aliphatic solvent such as hexane. For 2) it is important to have a
suitable rate of evaporation, so in the last case, hexane might evaporate too
fast, so I would go to a longer-chain hydrocarbon, e.g., decane. The Handbook
of Chemistry and Physics lists many compounds, both organic and inorganic, and
some solvents for each; I'd use that as a first reference. I hope this will
reduce the problem from a long series of trial-and-error efforts to a short one,
but you can still expect some problems. The preparation of suitable crystals is
most often the most difficult part of these studies. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us







From daemon Wed Jan 10 09:42:45 2001



From: Brian Gere :      vm89xt-at-zipee.net
Date: Wed, 10 Jan 2001 07:51:30 -0500
Subject: All of us #29E2

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From daemon Wed Jan 10 10:24:11 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 10 Jan 2001 10:19:32 -0600
Subject: xenon bulb problem

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I was forced to dismantle my Opti-quip fluorescence bulb housing the
other day for an unrelated problem. The 75W xenon bulb only has 200
hrs of use so I would have expected it to have another 200 hrs of
life based on what the supplier told me. But I noticed that at one
of the ends, at the junction between the metal cap and the glass
bulb, there was a buildup of a hard, white substance on the outside
of the glass. Does any one know what caused this and if it is safe
to re-install the bulb? TIA, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Jan 10 10:58:39 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 10 Jan 2001 14:55:42 -0200
Subject: Lauda Ultra-Kyomat K40D - Technical manual

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Hello all,

I am looking for the technical manual (components and operation) of the the
Lauda Ultra-Kryomat K 40 D. Can anybody help me?
Thank you.

Rinaldo Pires dos Santos
Dep. of Botany
UFRGS
Brazil



From daemon Wed Jan 10 11:53:57 2001



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Wed, 10 Jan 2001 11:50:42 -0600
Subject: Administrivia: Testing please ignore

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Colleagues:

This is just a test. I've had to make a revision to the JUNK mail
filter (we've been getting alot of hits lately) and I'm testing
the latest modification. To make sure real mail gets through.

Nestor
Your FriendlyNeighborhood SysOp.

===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================




From daemon Wed Jan 10 12:09:25 2001



From: Temporary Recruiter :      recruiter-at-thermonoran.com
Date: Wed, 10 Jan 2001 12:12:29 -0600
Subject: Job posting

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Microanalysis Applications Specialist
XRF Applications Specialist


Thermo NORAN, the makers of the finest instrumentation for elemental
analysis is seeking both a Microanalysis and an XRF Applications/Product
Specialist. This position will be responsible for the ensuring the success
of the applications/products targeted at analytical instrument markets,
including materials characterization and materials development markets.
Will perform demonstrations and provide other pre-sale support as required
at major trade shows, technical conferences and other meetings. Will
analyze customer samples and prepare reports for prospective customers.
Worldwide travel may be required to perform demonstrations and
presentations. Travel may include trips greater in duration than one week.
Must be degreed in a physical or life science, have excellent written and
oral communication skills. Knowledge of electron microscope and/or x-ray
microanalysis with experience in materials characterization.
Interested candidates should send, e-mail or fax resumes to:

Thermo NORAN
Attn: Matthew Duffy
2551 W. Beltline Hwy.
Middleton, WI 53562
e-mail: recruiter-at-thermonoran.com {mailto:recruiter-at-thermonoran.com}
fax: 608-836-7224
phone: 608-836-4382
www.thermonoran.com



From daemon Wed Jan 10 13:08:33 2001



From: Stephen Skirius :      Stephen_Skirius-at-bkitech.com
Date: Wed, 10 Jan 2001 13:00:20 -0600
Subject: TEM: Need help on the mounting/sectioning of cellulosic fibers fo

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Is there a "standard" procedure for the embedding, sectioning and staining
of wood fibers for examination by transmission electron microscopy?

Steve


From daemon Wed Jan 10 13:35:43 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 10 Jan 2001 17:36:39 -0200
Subject: Lauda Ultra-Kryomat K 40 D - Manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I am looking for the technical manual (components and operation) of the the
Lauda Ultra-Kryomat K 40 D. Can anybody help me?
Thank you.

Rinaldo Pires dos Santos
Dep. of Botany
UFRGS
Brazil




From daemon Wed Jan 10 14:56:10 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 10 Jan 2001 10:51:37 -1000 (HST)
Subject: Choosing a digital camera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A broad question for the light microscopists-

I'm writing up a wish list for our EM lab, and it includes (gasp) light
microscopes. My question is - how do I go about evaluating and choosing a
digital camera for light microscopes? It would be for both compound and
dissecting microscopes, should be color, decent resolution, not
necessarily low light nor real-time video, but capable of good images for
image analysis on sections. We are getting a confocal, so fluorescence
imaging would be done there rather than with the proposed 'scope and
camera.

What do I need to look for, and what price ranges are we talking about?

Mahalo,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jan 10 14:57:44 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 10 Jan 2001 12:54:59 -0800
Subject: Mounting large diatoms?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Does anyone know how to mount large (100um+) diatoms for LM or SEM? The
little ones are OK, they stick to most any stub, but these big guys like to
fall off.

Anyone know how arranged diatom slides are/were made? I checked WWW but no
specifics on techniques. Something like these methods would work for us.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Jan 10 17:16:50 2001



From: Douglas Keene :      DRK-at-shcc.org
Date: Wed, 10 Jan 2001 15:10:48 -0800 (Pacific Standard Time)
Subject: Nikon coolpix at low light levels

Contents Retrieved from Microscopy Listserver Archives
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Richard asked if the exposure meter on the Nikion coolpix
900 digital camera was sensitive enough to control exposure
in low light (immunofluorescent) conditions. We used the
camera to take images of samples labeled with moderate
intensely with FITC. We captured nice images in the "spot"
automatic metering mode. With the camera mounted in one
ocular, the camera chose to expose at F 3.1 and kept the
shutter open one second. However, it was a bit difficult
to use since the image did not appear on the display during
focusing. There was not enough light for real time
imaging. To insure a focused image, we needed focus in
brightfield illumination, then go to EPI to collect the
image. You could also use a monitor, but that would add
significant cost to the system.

It may be useful to realize that the camera has the ability
to be used in several automatic exposure settings
(programmed, aperture priority, shutter priority) (wide
field, narrow field, center spot) and in manual mode, where
you can use a timed exposure from 1/1000 to 8 seconds, or
longer (up to 60 seconds) by pushing the exposure once to
open and again to close.

Feel free to phone with more questions,

Doug

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Wed Jan 10 18:13:17 2001



From: Calvert, Dave :      calvert-at-eastman.com
Date: Wed, 10 Jan 2001 18:03:27 -0600
Subject: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers
My company's safety folks are bent on making us wear Kevlar gloves while
razor trimming samples for ultramicrotomy - I'm talking about the final
trimming done using a trimming stand on the microtome. As you might know,
this is fine work - wearing gloves is a handicap.
My questions:
1. Does anyone out there actually wear gloves? - if so then can you
recommend a glove?
2. Are there other ways to make this task look less dangerous to the
safety police? (short of purchasing a trimming machine)

Thanks!

Dave Calvert
Eastman Chemical Company
Building 150B Lincoln Street (packages)
P.O. Box 1972 (US Mail)
Kingsport, Tennessee 37662-1972
(423) 229-4943
(423) 229-4558 fax




From daemon Wed Jan 10 19:04:00 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 10 Jan 2001 15:00:49 -1000 (HST)
Subject: Re: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} My company's safety folks are bent on making us wear Kevlar gloves while
} razor trimming samples for ultramicrotomy - I'm talking about the final
} trimming done using a trimming stand on the microtome. As you might know,
} this is fine work - wearing gloves is a handicap.
} My questions:
} 1. Does anyone out there actually wear gloves? - if so then can you
} recommend a glove?
} 2. Are there other ways to make this task look less dangerous to the
} safety police? (short of purchasing a trimming machine)

When I train people to trim blocks, I always point to the Band-Aids first,
then to the razor blades! This gets a laugh, but drives home the
point. Then I suggest that they put the bandages on FIRST before trimming,
which also gets a laugh, but significant number of people decide to do
so! Therefore, I suggest something like finger cots or other wrap-around
solutions that leave some dexterity while protecting the last joints of
the fingers.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jan 10 21:36:22 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 10 Jan 2001 22:31:56 -0500
Subject: SEM Mounting of diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jonathan Krupp wrote:
==============================================================
Does anyone know how to mount large (100um+) diatoms for LM or SEM? The
little ones are OK, they stick to most any stub, but these big guys like to
fall off.

Anyone know how arranged diatom slides are/were made? I checked WWW but no
specifics on techniques. Something like these methods would work for us.
==============================================================
Consider these two options:

a) Tacky Dot™ Slides, see URL
http://www.2spi.com/new/tacky.html
and
b) Double sided conductive discs, sheets or tape
http://www.2spi.com/catalog/spec_prep/cond_adhes.html

The Tacky Dot Slides have good adhesion and the diatoms should be held in
place without problems. The double sided conductive sheets, discs, and tape
should work as well, but if you are using material that has aged past its
"expiration date", the surface tends to lose its "tac" and indeed might not
hold the "big guys". Of course, only the Tacky Dot Slides will result in
the diatoms being arranged in orthogonal arrays for convenient analysis.

Disclaimer: SPI Supplies is the exclusive worldwide licensee for the
manufacturing and distribution of Tacky Dot Slides for applications in
microscopy and microanalysis.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================









From daemon Thu Jan 11 00:09:30 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 11 Jan 2001 00:04:29 -0600
Subject: Re: Mounting large diatoms?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Klaus Kemp at http://www.diatoms.co.uk/index.html makes stunning
arrangments of diatoms and seems willing to share his knowledge.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}

}
} Hi:
}
} Does anyone know how to mount large (100um+) diatoms for LM or SEM? The
} little ones are OK, they stick to most any stub, but these big guys like
to
} fall off.
}
} Anyone know how arranged diatom slides are/were made? I checked WWW but
no
} specifics on techniques. Something like these methods would work for us.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}




From daemon Thu Jan 11 04:31:41 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 11 Jan 2001 20:24:40 +1000
Subject: RE: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Never cut myself in over quarter a century of trimming. Any accidental cuts
would be minor since little force is exerted during final trimming. Some force
is required during rough trimming and when cutting PE moulds off blocks -
without a press.
For these operations, just think where a slipped razor blade may go - and that
is were the fingers don't. Surprizingly hardly anybody in my labs ever cut a
finger, because they were taught the obvious.
I think its also obvious, that unless those Kevlar gloves are rather thick (no
analogy to the safety police) they would not prevent cuts. They should have
recommended those stainless steel chain linked butcher's gloves.
That could be a saleable idea!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, January 11, 2001 10:03 AM, Calvert, Dave
[SMTP:calvert-at-eastman.com] wrote:
}
}
}
} Listers
} My company's safety folks are bent on making us wear Kevlar gloves while
} razor trimming samples for ultramicrotomy - I'm talking about the final
} trimming done using a trimming stand on the microtome. As you might know,
} this is fine work - wearing gloves is a handicap.
} My questions:
} 1. Does anyone out there actually wear gloves? - if so then can you
} recommend a glove?
} 2. Are there other ways to make this task look less dangerous to the
} safety police? (short of purchasing a trimming machine)
}
} Thanks!
}
} Dave Calvert
} Eastman Chemical Company
} Building 150B Lincoln Street (packages)
} P.O. Box 1972 (US Mail)
} Kingsport, Tennessee 37662-1972
} (423) 229-4943
} (423) 229-4558 fax
}
}



From daemon Thu Jan 11 05:01:21 2001



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Thu, 11 Jan 2001 05:58:33 -0500
Subject: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have always worn latex gloves more for protection ( abrasion from
tightening chucks, etc.) Using long single edge razors (Weck Extra Long
Blades, EMS #71933-60) seems to give me more control of the blade and
therefore less of a chance to knick one's fingers.

Becky Garrison
Pathology Supervisor
Shand Jacksonville
Jacksonville, FL 32209
904-244-6237
-----Original Message-----
} From: Calvert, Dave [mailto:calvert-at-eastman.com]
Sent: Wednesday, January 10, 2001 7:03 PM
To: Microscopy-at-sparc5.microscopy.com


Listers
My company's safety folks are bent on making us wear Kevlar gloves while
razor trimming samples for ultramicrotomy - I'm talking about the final
trimming done using a trimming stand on the microtome. As you might know,
this is fine work - wearing gloves is a handicap.
My questions:
1. Does anyone out there actually wear gloves? - if so then can you
recommend a glove?
2. Are there other ways to make this task look less dangerous to the
safety police? (short of purchasing a trimming machine)

Thanks!

Dave Calvert
Eastman Chemical Company
Building 150B Lincoln Street (packages)
P.O. Box 1972 (US Mail)
Kingsport, Tennessee 37662-1972
(423) 229-4943
(423) 229-4558 fax




From daemon Thu Jan 11 06:05:46 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 11 Jan 2001 10:04:49 +0000
Subject: Re: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina

I have used LKB (now Reichert-Jung or whatever) microtomes and it has always been
possible to trim specimen blocks with glass knives on the microtome, itself. With
a bit of practice you can both trim and cut within about 30 minutes per block so I
don't think that it's particularly time consuming. The added bonuses are that
blocks will always have smooth facets and students get some good hands-on training
with microtome, binoculars and knives before actually cutting thin sections.

Once you get into the technique there are other rewards such as the ability to
'face' or smooth block fronts for best quality sections on difficult specimens,
mesa trimming, combining trimming of blocks and light microscopy surveys etc. The
only requirements are that your specimen must be embedded near to the surface of
the resin for easy trimming and that you don't damage the microtome mechanism.

The basic technique is fairly intuitive you just need practice:
trim the front of the block until you reach the specimen (1-5 um cuts on manual
feed should be OK)
rotate the knife stage by +10 to +40 deg (angle is optional and can be varied for
each side) and trim until you reach the desired edge in your block
rotate knife stage to the other side (i.e. -10 to -40deg) and again trim until you
have a long thin block face
rotate block in specimen holder by 90deg and repeat for the other two edges
Finally I may carefully trim or 'face' the front of the block for best results
If you want to produce mesas then don't rotate the knife - just use it to trim a
few microns around each side of the area to stand 'proud' as a mesa.
If the the block is difficult and blunts the knife you may need to use a second
knife, although this is not common
Smoothest trimming results from thinnest cuts at slowest speeds, so always make
the last couple of cuts on each edge about 1um or less.

If you're uncertain about the ability of your ultramicrotome to trim in this
fashion, I'm sure the manufacturer or someone on the list should be able to help.
If I do ever trim with a blade I use single edged razors and cut away from myself
on an uncluttered work area, but this may only happen once or twice a year often
when I'm demonstrating the method to students.

I'm sure that this has been mentioned on the list quite some time back but please
contact me directly if you have any further questions although there must be lots
of labs that have experience of this technique.

DISCLAIMER I have no commercial interest in LKB or Reichert, they just happen to
be the instruments that I have grown up with.

Good luck

Malcolm

Malcolm Haswell
e.m. unit
University of Sunderland
UK


Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } My company's safety folks are bent on making us wear Kevlar gloves while
} } razor trimming samples for ultramicrotomy - I'm talking about the final
} } trimming done using a trimming stand on the microtome. As you might know,
} } this is fine work - wearing gloves is a handicap.
} } My questions:
} } 1. Does anyone out there actually wear gloves? - if so then can you
} } recommend a glove?
} } 2. Are there other ways to make this task look less dangerous to the
} } safety police? (short of purchasing a trimming machine)
}
} When I train people to trim blocks, I always point to the Band-Aids first,
} then to the razor blades! This gets a laugh, but drives home the
} point. Then I suggest that they put the bandages on FIRST before trimming,
} which also gets a laugh, but significant number of people decide to do
} so! Therefore, I suggest something like finger cots or other wrap-around
} solutions that leave some dexterity while protecting the last joints of
} the fingers.
}
} Aloha,
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************



From daemon Thu Jan 11 06:09:20 2001



From: Tiffany Argent :      tiffany.argent-at-abbott.com
Date: Thu, 11 Jan 2001 06:06:32 -0600
Subject: Cross section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to find a way of looking at a cross section of a printed ink
approx 10um thick on a PVC substrate. I am trying to measure the porosity of
the ink so I guess some image analysis will be used. It is mainly a method
of preparing the samples that has me a bit stumped. I have tried freeze
fracturing and embedding the samples in resin then polising the surface.
Neither are satisfactory. The microscopy techniques I have available are
SEM and LM. Can anyone help?


From daemon Thu Jan 11 06:51:09 2001



From: Michael P. Goheen :      mgoheen-at-iupui.edu
Date: Thu, 11 Jan 2001 07:56:41
Subject: LM Stain

Contents Retrieved from Microscopy Listserver Archives
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Jon -

Largish diatoms, like most other microfossils bigger than, say, 50
microns or so in diameter or length, can usually be handled under a
binocular microscope with a very fine (00 or 000) artists' paintbrush. Just
wet the brush with water (distilled, if it's going to matter) and touch the
bug with it. It'll stick to the brush long enough for you to place it on a
stub. When I'm looking at microfossils in the SEM, I can usually get them to
stick to double-sided tape on a stub just by "dabbing" them on with the
point of the brush. Orienting them can be a bit tricky, but you'll improve
with practice. One problem with double-sided tape, though, is that once
stuck on, a lot of the more delicate microfossils such as diatoms will break
before they'll come loose. So you don't want to handle type specimens this
way....
Another good way to stick such bugs down includes a little bit of Gum
Tragacanth. An old micropaleontologists' standby, this stuff is available as
a powder, which you mix with a little water to make a paste. You can glue
individual bugs down with this, using your fine paint brush. It dries in a
few seconds - and if you want to move/remove the bug later, just wet your
brush and touch the bug. The gum redissolves and the specimen comes free.
Unsightly gum residues on the bug will come off with a bit more application
of your wet brush. Once mixed up and kept in a sealed little vial, the gum
mixture should keep for months or years. A drop or two of clove oil in it
will inhibit any possible mould growth, if you're going to keep it around
for a while.
One guy I used to work with liked to use bits of old undeveloped 35mm
film. Apparently you can get small items to stick to such film really well
if the specimen is wet. I've never tried this myself, though.
And, of course, there's lots of micropaleontology texts out there with
chapters on handling techniques.
After reading all this, you're probably sorry you got me started.
Anyway, good luck with the diatoms - they're among nature's most photogenic
micro-organisms.

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 10, 2001 4:54 PM


Hi Folks,

Does anyone know of a stain to differentiate PMN's from Macrophages for
light microscopy?

Thanks,

Mike Goheen



From daemon Thu Jan 11 07:59:49 2001



From: Judy Murphy :      jmurphy-at-sjdccd.cc.ca.us
Date: Thu, 11 Jan 2001 07:54:21 -0600
Subject: Delta Microscopy Society, & Northern California Microscopy

Contents Retrieved from Microscopy Listserver Archives
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We are honored to host

Dr. Patrick Echlin of Cambridge University, UK

for a 7 Hr Minicourse in Cryomicroscopy and Microanalysis

at the Microscopy Technology Center of San Joaquin Delta College in
Stockton, CA

Everyone is welcome. Come, enjoy, and RSVP.
Happy New Year

Delta Microscopy Society, & Northern California Microscopy Society
Joint Meeting



WHEN: Jan 17, 18, 2001; Wed. and Thur.

WHERE: San Joaquin Delta College, Microscopy Technology Center, Stockton, CA

Please email or fax RSVP. See form below.

7 Hr Minicourse in Low Temperature Microscopy and Microanalysis
by Dr. Patrick Echlin, Director of Cambridge Analytical Microscopy and
member of Multi-Imaging Centre, University of Cambridge, UK

Image Database (Asset Management System) for Multi-Platform, Multi-Instrument
Microscopy Laboratory, Dr Judy A. Murphy, San Joaquin Delta College,
Microscopy Technology Center

January 17, 2001: Wednesday
12:00 - 1:00 pm Registration, Holt 121
1:15 - 1:30 pm Welcome and Introduction, South Forum Building
1:30 - 2:30 pm Minicourse Lecture 1 : Chemical-physics of water and ice
2:45 - 3:30 pm Minicourse Lecture 2: Sample cooling
3:30 - 4:30 pm Tour of Microscopy Technology Center, Holt 121
including Demo of Image Database
4:30 - 5:30 pm Minicourse Lecture 3; Sectioning and Fracturing
Discussion
Social 5:30 - 6:30 pm, Budd Lounge
Light Buffet 6;30 - 8pm, Budd Lounge
8:00 - 9:00 Image Database Lecture

January 18, 2001: Thursday
8:00 - 8:45 am Registration, 121 Holt
9:00 - 10:00 am Minicourse Lecture 4: Freeze drying, freeze substitution
and low temperature embedding
10:15 - 11 am Minicourse Lecture 5: Low temperature microscopy
Discussion
11:15 - 1:00 Lunch
1:15 - 2:15 pm Minicourse Lecture 6: Low temperature microanalysis
2:30 - 3:30 pm Minicourse Lecture 7 : Imaging, artefacts and beam damage.
Discussion

Any building or room changes will be posted in Holt 121.

Activities are being sponsored by: Gatan Inc., Oxford Instruments, Hitachi,
JEOL, FEI, Ted Pella Inc., EMS/Diatome, Anatech, Cressington, LEO, Advanced
Computers and Networking, Delicato Winery, Paul De Georgio and others to be
identified later.

Please RSVP so we know what room sizes we will need, # of handouts, and
how much food.


Cost to Students: $0
Cost to Other Registrants $15 (to defray cost of activities, handouts, etc.)

Please PRINT or TYPE

Name:

Company:

Address:

City, State, ZIP

Phone:

FAX:

email:

Activities you will attend: Please indicate which you will attend:

Wed. afternoon lectures: yes no maybe

Wed. Social/Buffet yes no maybe

Wed. pm Lecture yes no maybe

Thur. am Lectures yes no maybe

Thur pm Lectures yes no maybe

Please email or fax RSVP

email to:
murphyjudy-at-mediaone.net

FAX to: 209/954-5600
This is a central fax, so put Judy Murphy, Microscopy on top of fax.

Any questions, call
Judy Murphy (209/954-5284) or Pat Kysar (209/954-5246)

Checks can be made out to Delta Microscopy Society and sent to:
Judy Murphy, San Joaquin Delta College, Microscopy Technology Center, 5151
Pacific Ave, Stockton, CA 95207

Directions to Microscopy Technology Center & Lodging
Holt Center
San Joaquin Delta College
5151 Pacific Avenue
Stockton, CA 95207

1. Find Interstate 5 (I-5) on California map. It is a main North/South
Interstate in California.
2. From I-5, exit on March Lane going East. Continue on March Lane 1.6
miles until you reach the traffic light at Pacific Ave.
3. At Pacific Ave.turn LEFT, and go 0.3 miles until you reach the traffic
light for Delta College.
4. Turn LEFT at Delta College Entrance. The street is labeled Yoguts going
East and Delta College going West.
5. As soon you turn into Delta College Entrance, bear LEFT on the Campus
Drive (called Burke-Bradley in some places).
6. Continue around on Campus Drive until you reach Holt 1 Parking Lot.
Turn RIGHT into Holt 1 Parking Lot.
7. As soon as you turn into Holt 1 Parking Lot, take a sharp RIGHT turn to
enter Holt 2 Parking Lot.
8. Park as close to the front of the parking lot as possible, closest to
the buildings.
9. Since this is the first week of Spring semester classes, no tickets are
issued, so you do NOT need a parking permit.
10. As you walk towards the buildings, you should first enter Holt
building. Go to Holt 121 which is the Microscopy Center.
11. Any questions, call Judy Murphy (209/954-5284) or Pat Kysar
(209/954-5246)

Lodging Within 1.5 miles of San Joaquin Delta College
Prices were quoted Dec. 20, 2000, but check when you make reservations.
All listed lodging is close to March Lane exit off Interstate 5

Red Roof Inn, 2654 March Lane, Stockton, CA, Phone: 209/478-4300
Fax: 209/478-1872; Rates: Single - $59.99; Queen or 2 Double - $65.99;
King - $71.99; Suite - $87 and up depending on number of people.

LaQuinta, 2710 W. March Lane, Stockton, CA, Phone: 209/952-7800, Fax:
209/472-0732; Rates: King-$75; 2 Double Beds-$69, Complimentary Breakfast
AAA, AARP,Senior Citizen Discount Rates: King-$71.25; 2 Double beds-$65.55

Radisson Hotel, 2323 Grand Canal Blvd., Stockton, CA, Phone 209/957-9090
Fax: 209/473-0739; Rates: $79 - $99. Canal Blvd is one block off March Lane.

Courtyard by Marriott, 3252 W. March Lane, Stockton, CA, Phone
209/472-9700, Fax:
209/472-9722; Rate: $89-104
.
Marriott Residence Inn, 3240 W. March Lane, Stockton, CA, Phone: 209/472-9800
Fax: 209/472-9888; Rates: Studio - $119; 1 Bedroom - $124. Complimentary
Breakfast every day. Rooms have Kitchens equipped with Stove,
refrigerators, pots & pans etc




From daemon Thu Jan 11 10:05:46 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 11 Jan 2001 10:00:58 -0600
Subject: Re: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
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It sounds like the safety folks may be overzealous in their attempts
to protect you from embedding resins.
Once polymerized, the resins are considerably safer than the
monomers. Since the plastic is no longer liquid, latex gloves would
be more appropriate (if the individual is not allergic to latex).

A bigger concern, I would think, when working with polymerized
plastic is the possible allergic reaction due to inhalation of the
plastic chips. I strongly recommend the use of a dust mask if you are
using a grinder or other mechanical device to shape the blocks.




} My company's safety folks are bent on making us wear Kevlar gloves while
} razor trimming samples for ultramicrotomy - I'm talking about the final
} trimming done using a trimming stand on the microtome. As you might know,
} this is fine work - wearing gloves is a handicap.
} My questions:
} 1. Does anyone out there actually wear gloves? - if so then can you
} recommend a glove?
} 2. Are there other ways to make this task look less dangerous to the
} safety police? (short of purchasing a trimming machine)
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Jan 11 10:30:55 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 11 Jan 2001 17:27:45 +0100
Subject: EDS detector pumping

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Hello

I have no experience in EDS detector pumping, but a little bit in vacuum
technology.

What do you think about heating the Dewar before pumping ?

That's what I do with sorbtion (zeolite) pumps used to prepump UHV vessel
(to have a dry pumping before starting turbo or ion pump). I put a heater
around the pump -in our case it would be hot water (hot air ? hot oil ?
which temperture can support the Dewar assembly ?)- let it warm over the
night, and close the venting valve.

You could do something similar with the detector : warm it at air
pressure for a few hours (it schould dry too the zeolite) and than pump
down with turbo, but pre-pump with dry backing pump to prevent oil
contamination,
as Jim mentioned. Than pump down until the Dewar is cold, with a base
pressure of something like 10E-5 Torr. And after that, cool it with
nitrogen.

What do you think about that ?

Bye


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Jan 11 10:34:00 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 11 Jan 2001 10:30:17 -0600
Subject: Re: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
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}
}
} } My company's safety folks are bent on making us wear Kevlar gloves while
} } razor trimming samples for ultramicrotomy
} } 2. Are there other ways to make this task look less dangerous to the
} } safety police? (short of purchasing a trimming machine)


We routinely use a Dremel moto-tool (available at your local hardware
store) with thin cutting wheels (i prefer the ones from Small Parts,
Inc that have an abrasive on one side and a very thin metal backing
on the other side). Working in the hood to avoid the dust, we can
trim dozens of blocks rapidly. I advise holding the block in a
pliers in case you slip with the dremel but it really is very easy
once you get used to it.
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Jan 11 10:57:14 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 11 Jan 2001 17:51:52 +0100
Subject: Thikness measurment

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Hello Jondo

If someone in your lab has a Perrot Fabry spectrometer, you can use it to
mesure film thickness between 2 and 100 nm. You évaporate your film on a
glass slide (LM slide), than you put a fine scratch on the film, than you
evaporate a opticaly opaque film of silver or aluminum. Than on the Perrot
Fabry, with a monochromatic light, you can see and mesure the hights of
the siver setp, which covers the the film to bee mesured. You must know
the wavelength of your light. You schould find the way to calculate this
in any optics book. You must do a serie of different thickness to draw a
calibration curve. Its very sensitiv and cheep,... if you have the Perrot
Fabry of course

An other way, opticaly too, is on a LM with a Nomarski Prism. I used it 15
years ago, but I do know the way to make the optical montage. I remember
that there is a monochromatique light, the Nomarski prism, the polariser
and analyser, but what kind of objectif ? The sample must be scratched and
silver covered as with the Perrot Fabry...

Can someone tell me something about that ?

Thanks


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Jan 11 11:18:39 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 11 Jan 2001 17:14:30 +0000
Subject: Re: 4by5 film scanners

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George

I had a quick look at the specification of the Coolscan 8000ED and it seems
to be aimed at TEM users with about 6x9cm films - I quote the largest areas
from the web page:
(6 x 9) 63.5 x 88.0mm (10,000x13,176 pixels);
(Elect Micro) 56.9 x 83.7mm (8,964x13,176 pixels);
My e.m. film is 4 x 3 5/16 inches or 83 x 102 mm with an absolute minimum
picture area (without data) of 69 x 92mm so it may be a problem for some e.m.

users.

I must admit it's a great specification though.

Malcolm Haswell
e.m. unit
University of Sunderland
UK

George Laing wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} All,
} The preliminary information I have is that the Coolscan 8000 ED
} will sell for approximately $4000 US. We will carry it when it becomes
} available, currently listed as June. I hope to see it in early February.
} Quick specs..
} 4000dpi optical resolution
} IEEE1394 "Firewire" Interface- not SCSI
} 4.2 dynamic range 48bit images
} Multi Sample Scanning
}
} Info is also at
} http://64.77.39.20/usa_product/product.jsp?cat=7&grp=703&productNr=9246
}
} I'll pass along more info as it becomes available.
}
} George
}
} George Laing
} National Graphic Supply
} v:(800) 223-7130 X3109
} f:(800) 832-2205
} email: scisales-at-ngscorp.com
} }
} } This looked pretty interesting. Have you heard a ballpark price?
} }
} } }
} } } In the context of recent queries, I just thought I'd
} } } post Nikon's most recent announcement ... see:
} } } http://www.klt.co.jp/Nikon/Press_Release/ls-8000.html
} } }
}
} }



From daemon Thu Jan 11 11:30:12 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Jan 01 09:36:22 -0800
Subject: RE: Ultramicrotomy safety issues

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Jan 01 09:36:22 -0800
Subject: RE: Ultramicrotomy safety issues

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Reply to: RE: Ultramicrotomy safety issues
It is possible to perform all the trimming steps needed to get a good block by using glass knives. Not only is it safer for the operator it also produces blocks with smooth, regular edges which are great for serial sectioning. More details provided on command.

Paul Webster.




Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Calvert, Dave wrote:
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} -----------------------------------------------------------------------.
}
}
} Listers
} My company's safety folks are bent on making us wear Kevlar gloves while
} razor trimming samples for ultramicrotomy - I'm talking about the final
} trimming done using a trimming stand on the microtome. As you might know,
} this is fine work - wearing gloves is a handicap.
} My questions:
} 1. Does anyone out there actually wear gloves? - if so then can you
} recommend a glove?
} 2. Are there other ways to make this task look less dangerous to the
} safety police? (short of purchasing a trimming machine)
}
} Thanks!
}
} Dave Calvert
} Eastman Chemical Company
} Building 150B Lincoln Street (packages)
} P.O. Box 1972 (US Mail)
} Kingsport, Tennessee 37662-1972
} (423) 229-4943
} (423) 229-4558 fax
}
}
}
}
}
} RFC822 header
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From daemon Thu Jan 11 11:47:09 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Thu, 11 Jan 2001 09:25:51 -0800
Subject: STEM

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Dear Colleagues,

Does anyone know any place that has a TEM with a dedicated STEM unit
attached. The reason for the question is that I have particles composed of
cores and shells (coated particles), both of which are different materials.
By TEM I am not able to discern the shells from the cores because the
contrast is extremely low. However, I am told that a dedicated STEM unit
might allow me distinguish the shells from the cores based on the fact that
they both have very different z-contrast.

Regards,
Thearith

_________________________

Thearith Ung, Ph.D.
Quantum Dot Corporation
26136 Research Road
Hayward CA 94545
Tel: (510)-887-8775 (x4125)
Fax:(510)-783-9729
www.qdots.com



From daemon Thu Jan 11 11:57:08 2001



From: Lewis McCrigler :      lmm7001-at-Humboldt.edu
Date: Thu, 11 Jan 2001 09:56:34 -0800
Subject: Help on an Orthomat E Photo System

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I'm looking for technical assistance or a service
manual for a
Leitz
Orthomat E (7916) Photo System.The problem we are
having is
the unit will
not allow a picture to be taken unless there is very
little light.
Once the
system thinks it is in range, there is not enough
light present to
get the
photograph. An adjustment of the light detector? Any
assistance
is
appreciated.
Lewis McCrigler
Humboldt State University



From daemon Thu Jan 11 12:16:53 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 11 Jan 2001 12:13:16 -0600
Subject: RE: Cross section sample preparation

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I've never worked with ink samples, but nevertheless I am
trying to give some advice (be cautious!).

I think that techniques like freeze fracturing are not suitable for
your purposes. Cracks could prefer to propagate through pores, so
fracture surface will exaggerate porosity. Polished cross sections are
much better for quantification. Maybe you could find suitable media for
embedding. And what about mounting 2-3 substrates in a clip and cutting
them with a sharp blade (hm... I really do not know ink's properties...)?

Vladimir



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Tiffany Argent [mailto:tiffany.argent-at-abbott.com]
} Sent: Thursday, January 11, 2001 6:07 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Cross section sample preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
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} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
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} ---------.
}
}
} I am trying to find a way of looking at a cross section of a
} printed ink
} approx 10um thick on a PVC substrate. I am trying to measure
} the porosity of
} the ink so I guess some image analysis will be used. It is
} mainly a method
} of preparing the samples that has me a bit stumped. I have
} tried freeze
} fracturing and embedding the samples in resin then polising
} the surface.
} Neither are satisfactory. The microscopy techniques I have
} available are
} SEM and LM. Can anyone help?
}


From daemon Thu Jan 11 13:15:53 2001



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 11 Jan 2001 12:58:33 -0500
Subject: fluorescence intensity ratios

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One of our investigators is studying the ratio of total and phosphorylated
transcription factor in the cell. We are able to do multiple fluorescence
labeling but I need help in determining the fluorescence intensity ratios.
We are using the Alexa dyes 350, 488 and 630 just to make sure there is no
bleed through with the filters that we use. I would like to know if anyone
has done this type of experiment and what are the potential problems that
one could run into. I would appreciate comments or suggestions.

Thank you,

Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Thu Jan 11 13:20:06 2001



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Thu, 11 Jan 2001 13:18:56 -0600
Subject: RE: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
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We've only lost about 4 technologists due to them accidentally cutting open
their jugulars with a razor blade whilst trimming blocks and bleeding to
death on the spot. So with that good safety record, we've chosen to
dispense with the gloves thus far. Should we lose a few more though, the
issue might come up again.

Garry

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg, Canada



From daemon Thu Jan 11 14:34:36 2001



From: Louise_Harner-at-albint.com
Date: Thu, 11 Jan 2001 15:28:51 -0500
Subject: Re: Mounting large diatoms?

Contents Retrieved from Microscopy Listserver Archives
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Jon -

I've never done any serious particle arranging, but perhaps one of the people
mentioned below can help you.

You might try asking Klaus Kemp via the website at http://www.diatoms.co.uk
since he creates arranged slides as a profession. He has been referred to as a
master of the art at several microscopy seminars I've attended.

http://www.microscopy-uk.org.uk/mag/artapr00/machslide.html will take you to an
article titled "A microscopical view of an old diatom circle pattern slide with
250+ diatom shells" by Martin Mach (there is a link to his e-mail). He provides
a "Modern Microscopy" reference for preparation of such slides, but unless you
have a GOOD library, you might ask him if he could send you a copy of the
article - it is dated 1895.

Anna Teetsov at McCrone Associates (http://www.mccrone.com/ma/staff.html) is
also expert at positioning particles on slides. While she is famous for
manipulating the tiniest of particles, I've seen some beautiful arranged
butterfly scale slides she's made for viewing under the light microscope. She
might be willing to provide some suggestions.


Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com





jmkrupp-at-cats.
ucsc.edu (Jon To: Microscopy-at-sparc5.microscopy.com
Krupp) cc:
Subject: Mounting large diatoms?
2001/01/10
03:54 PM






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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi:

Does anyone know how to mount large (100um+) diatoms for LM or SEM? The
little ones are OK, they stick to most any stub, but these big guys like to
fall off.

Anyone know how arranged diatom slides are/were made? I checked WWW but no
specifics on techniques. Something like these methods would work for us.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu








From daemon Thu Jan 11 14:50:19 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 11 Jan 2001 14:46:26 -0600
Subject: Microtome for semi-thins

Contents Retrieved from Microscopy Listserver Archives
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A colleague (visiting from Brazil) is looking for a microtome for
cutting semi-thin (0.25 to 2 micrometer) sections of plastic (epoxy)
embedded specimens (mammalian tissues). He is considering a new Leica
RM2165 ($18K).

I am soliciting testimonials (good and bad) for this or ANY other
microtomes for cutting semi-thins.

Thank you on his behalf.

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Jan 11 15:00:44 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 11 Jan 2001 14:54:31 -0600
Subject: RE: Ultramicrotomy safety issues

Contents Retrieved from Microscopy Listserver Archives
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Garry,

We've solved that problem with Kevlar collars. We found that gloves just
made it MORE likely that jugulars would be severed, so we don't use them
either.

Randy

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Thursday, January 11, 2001 1:19 PM
To: microscopy-at-sparc5.microscopy.com


We've only lost about 4 technologists due to them accidentally cutting open
their jugulars with a razor blade whilst trimming blocks and bleeding to
death on the spot. So with that good safety record, we've chosen to
dispense with the gloves thus far. Should we lose a few more though, the
issue might come up again.

Garry

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg, Canada



From daemon Thu Jan 11 15:28:26 2001



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Thu, 11 Jan 2001 15:50:36 -0500
Subject: SAFETY POLICE

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Dave:

In my 21 years of thin sectioning I have from time to time cut my fingers,
but a band aid is all that was needed. Kevlar gloves sound to me like
ridiculous over kill. However for those of us working in really big and not
always friendly cities, there might be an argument for Kevlar vests. Happy
sectioning, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Thu Jan 11 15:33:16 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 11 Jan 2001 15:29:53 -0600
Subject: Re: Ultramicrotomy safety issues

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----- Original Message -----
} From: "Garrison, Becky" {becky.garrison-at-jax.ufl.edu}
} I have always worn latex gloves more for protection ( abrasion from
} tightening chucks, etc.) Using long single edge razors (Weck Extra Long
} Blades, EMS #71933-60) seems to give me more control of the blade and
} therefore less of a chance to knick one's fingers.
}
I have at times shaved with a straight razor and that is little different
that what a long single edge razor. Some people that have beards still do
although a old Gillette safety razor with the guard removed is easier to
use since sharpening a straight razor is a pain. Explaining that some
folks still use them to shave should at least catch the safely Nazi off
guard if not convince him of the absurdity of his request.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Thu Jan 11 15:56:17 2001



From: William R. Oliver :      oliver-at-cpt.afip.org
Date: Thu, 11 Jan 2001 14:29:59 -0500 (EST)
Subject: Using a microscope as a "flatbed" scanner

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Hi! I am interested in using a light
microscope to digitize photographic film, i.e.
to use my light microscope as a film scanner
to scan negatives.

Since our lab does a fair amount of investigation
into telepathology applications, we have scopes
that are set up for the automatic scanning of
multiple fields, merging of fields, etc. That
problem is (relatively) solved.

What I am getting stumped on here is how to
mount my 35mm photographic negatives. If I
just slap one on a slide and set a coverslip
on top, I get (not surprisingly) nontrivial
refractive problems with irregularities in the
surface of the film.

Unfortunately, these are photographic negatives
which might have forensic/evidentiary value, so
I can't dribble on any of the mounting media I
can think of -- since I can't permanently mount
the negative. I have to be able to take that
negative at a later time and make an "original"
photograph.

Anybody have experience with this? Any lore
would be appreciated.


billo




From daemon Thu Jan 11 16:49:14 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 11 Jan 2001 17:44:52 -0500
Subject: Re: TEM Course

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A one day TEM course sponsored by the AVS will given during the upcoming
February Santa Clara meeting. For more information please see
www.vacuum.org


At 9:04 AM -0800 12/13/00, Thearith H. Ung wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Thu Jan 11 18:59:10 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 12 Jan 2001 10:52:58 +1000
Subject: RE: Using a microscope as a "flatbed" scanner

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Glycerin has about the right refractive index and is easily washed out with
water, after which the negatives can be air dried. Certainly glycerin works as
a temporary mountant for microscope slides and the negative's gelatin emulsion
is not affected by it.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, January 12, 2001 5:30 AM, William R. Oliver
[SMTP:oliver-at-cpt.afip.org] wrote:
}
}
}
} Hi! I am interested in using a light
} microscope to digitize photographic film, i.e.
} to use my light microscope as a film scanner
} to scan negatives.
}
} Since our lab does a fair amount of investigation
} into telepathology applications, we have scopes
} that are set up for the automatic scanning of
} multiple fields, merging of fields, etc. That
} problem is (relatively) solved.
}
} What I am getting stumped on here is how to
} mount my 35mm photographic negatives. If I
} just slap one on a slide and set a coverslip
} on top, I get (not surprisingly) nontrivial
} refractive problems with irregularities in the
} surface of the film.
}
} Unfortunately, these are photographic negatives
} which might have forensic/evidentiary value, so
} I can't dribble on any of the mounting media I
} can think of -- since I can't permanently mount
} the negative. I have to be able to take that
} negative at a later time and make an "original"
} photograph.
}
} Anybody have experience with this? Any lore
} would be appreciated.
}
}
} billo
}
}



From daemon Thu Jan 11 19:07:35 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 12 Jan 2001 11:05:55 +1000
Subject: RE: EDS detector pumping

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Don't do it. The reason for pumping detectors first without heat is to avoid
further contamination of the crystal. When the dewar is heated material is
released from the walls of the contained space and especially the molecular
sieve.
Pump first and then heat, that way the pump can cope and quickly eliminate the
additional contaminants.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, January 12, 2001 2:28 AM, Faerber Jacques
[SMTP:Jacques.Faerber-at-ipcms.u-strasbg.fr] wrote:
}
} Hello
}
} I have no experience in EDS detector pumping, but a little bit in vacuum
} technology.
}
} What do you think about heating the Dewar before pumping ?
}
} That's what I do with sorbtion (zeolite) pumps used to prepump UHV vessel
} (to have a dry pumping before starting turbo or ion pump). I put a heater
} around the pump -in our case it would be hot water (hot air ? hot oil ?
} which temperture can support the Dewar assembly ?)- let it warm over the
} night, and close the venting valve.
}
} You could do something similar with the detector : warm it at air
} pressure for a few hours (it schould dry too the zeolite) and than pump
} down with turbo, but pre-pump with dry backing pump to prevent oil
} contamination,
} as Jim mentioned. Than pump down until the Dewar is cold, with a base
} pressure of something like 10E-5 Torr. And after that, cool it with
} nitrogen.
}
} What do you think about that ?
}
} Bye
}
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Materiaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Jan 11 23:01:08 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 11 Jan 2001 22:56:13 -0600
Subject: Re: Using a microscope as a "flatbed" scanner

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There is also a film cleaning fluid I found several at Porters Camera
http://www.porters.com/ and search for "film cleaner" in their on line
store. These usually don't require washing and would be easier to clean
off than Glycerin. There is a scratch remover that might also work. I
couldn't find it so it may have had something in it that the EPA didn't
like. Your darkroom or local camera store may have these.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
} From: "Jim at ProSciTech" {jim-at-proscitech.com}
}
} Glycerin has about the right refractive index and is easily washed out
with
} water, after which the negatives can be air dried. Certainly glycerin
works as
} a temporary mountant for microscope slides and the negative's gelatin
emulsion
} is not affected by it.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Friday, January 12, 2001 5:30 AM, William R. Oliver
} [SMTP:oliver-at-cpt.afip.org] wrote:
} }
} }
} }
} } Hi! I am interested in using a light
} } microscope to digitize photographic film, i.e.
} } to use my light microscope as a film scanner
} } to scan negatives.
} }
} } Since our lab does a fair amount of investigation
} } into telepathology applications, we have scopes
} } that are set up for the automatic scanning of
} } multiple fields, merging of fields, etc. That
} } problem is (relatively) solved.
} }
} } What I am getting stumped on here is how to
} } mount my 35mm photographic negatives. If I
} } just slap one on a slide and set a coverslip
} } on top, I get (not surprisingly) nontrivial
} } refractive problems with irregularities in the
} } surface of the film.
} }
} } Unfortunately, these are photographic negatives
} } which might have forensic/evidentiary value, so
} } I can't dribble on any of the mounting media I
} } can think of -- since I can't permanently mount
} } the negative. I have to be able to take that
} } negative at a later time and make an "original"
} } photograph.
} }
} } Anybody have experience with this? Any lore
} } would be appreciated.
} }
} }
} } billo
} }
} }
}
}




From daemon Fri Jan 12 01:44:31 2001



From: Nuria Cortadellas :      nuriac-at-giga.sct.ub.es
Date: Fri, 12 Jan 2001 08:39:23 +0100 (CET)
Subject: callose

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Hi, microscopists

does anybody knows the telephone number from Biosupplies (Melbourne,
Australia), I bought a callose ((1-3)beta-glucan-specific) monoclonal
antibody two years ago and I can't contact with them at the telephone that
I have.
Thanks a lot
Nuria

------------------------------------------------------------------------------
Nuria Cortadellas
Unitat de Microscopia de Transmissio
Serveis Cientifico-Tecnics
Universitat de Barcelona
C/ Sole i Sabaris, 1-3 08028 Barcelona
Tel: +34 3 402 13 52
Fax: +34 3 402 13 98
E-mail: nuriac-at-giga.sct.ub.es



From daemon Fri Jan 12 02:16:19 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 12 Jan 2001 08:13:20 +0000 (GMT Standard Time)
Subject: Re: SAFETY POLICE

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We put up with the minor wounds. I do however tell
students not to cut themselves as I will be obliged to fill
in an accident form and the safety officer will invite me
to think of alterative methods. We thought of blunting the
blade corners or making little corner guards but never got
round to it.

Dave

On Thu, 11 Jan 2001 15:50:36 -0500 Timothy Schneider
{Timothy.Schneider-at-Mail.TJU.EDU} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dave:
}
} In my 21 years of thin sectioning I have from time to time cut my fingers,
} but a band aid is all that was needed. Kevlar gloves sound to me like
} ridiculous over kill. However for those of us working in really big and not
} always friendly cities, there might be an argument for Kevlar vests. Happy
} sectioning, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jan 12 04:22:27 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 12 Jan 2001 10:18:45 +0000
Subject: Re: STEM

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Thearith

we use a Hitachi H7000 with STEM attachment although I'm sure there will be
someone much closer to you, but I would like to make a couple of comments:
you mention a dedicated STEM unit which to me is a free-standing high
resolution STEM and not a STEM attachment to a TEM. There should be lots of
TEMs with STEM attachments on instruments bought in the last 10 or 15 years but
only a few high resolution STEMs because they are expensive and specialized
(try Brookhaven National Laboratory website
http://bnlstb.bio.bnl.gov/biodocs/stem/stem.htmlx).

You may also want to consider that certain TEMs have built in energy filtering
or electron energy spectrometers which can actually be tuned for different
atomic masses in the specimen - Zeiss EM902s can do this but I'm not sure if
any other machines are available.

I have no connection with Brookhaven or Zeiss, although of course I do use
Hitachi.

Good luck

Malcolm


Malcolm Haswell
e.m. unit
University of Sunderland
UK

"Thearith H. Ung" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Colleagues,
}
} Does anyone know any place that has a TEM with a dedicated STEM unit
} attached. The reason for the question is that I have particles composed of
} cores and shells (coated particles), both of which are different materials.
} By TEM I am not able to discern the shells from the cores because the
} contrast is extremely low. However, I am told that a dedicated STEM unit
} might allow me distinguish the shells from the cores based on the fact that
} they both have very different z-contrast.
}
} Regards,
} Thearith
}
} _________________________
}
} Thearith Ung, Ph.D.
} Quantum Dot Corporation
} 26136 Research Road
} Hayward CA 94545
} Tel: (510)-887-8775 (x4125)
} Fax:(510)-783-9729
} www.qdots.com



From daemon Fri Jan 12 04:51:15 2001



From: Peter Bond :      P.Bond-at-plymouth.ac.uk
Date: Fri, 12 Jan 2001 08:17:03 -0600
Subject: Microtome injury

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Glycerin, and also ethylene glycol, can be dried under vacuum
making it possible to avoid rehydrating the negative
Chris

----- Original Message -----
} From: "Jim at ProSciTech" {jim-at-proscitech.com}
To: "'William R. Oliver'" {oliver-at-cpt.afip.org} ;
{microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 12, 2001 12:52 AM


Before this subject is banned from the list -

Our only serious injuries to date are blinded students falling asleep
while sectioning and poking their eyes out on the binocular eyepieces!

Perhaps the kevlar collars would keep their heads up a little longer?!

Pete

--
Peter Bond
Plymouth Electron Microscopy Unit
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel/Fax: 01752 233092
email: pbond-at-plymouth.ac.uk




From daemon Fri Jan 12 09:55:42 2001



From: ed_bachmann-at-unc.edu (Ed Bachmann)
Date: Fri, 12 Jan 2001 11:01:48 -0500 (Eastern Standard Time)
Subject: Nikon SMZ800 vs Leica MZ7 Stereozooms

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I am trying to judge the optical quality of two similar stereoscopic zoom
scopes: the Nikon SMZ800 and the Leica MZ7, both equipped with a 1x
Planapochromatic objective.

Leica publishes resolution figures for the MZ7, claiming 309 line pairs per mm.
Nikon does not, and I haven't been able to persuade the Nikon representative to
"characterize" the resolution of the SMZ800, although he says he can. I do not
have the necessary test slide, nor do I know where I can get one with a scale
graduated from say 200-400 lp/mm. I do know though that such a slide would cost
probably $300-$500.

Interestingly, Nikon publishes a resolution number for its next higher quality
scope, the SMZ1000. It claims the SMZ1000 can resolve 300 lp/mm with a 1x
Planapo. Because Nikon product literature doesn't publish a resolution for the
SMZ800, I suspect that its resolution is substantially lower than 300 lp/mm, and
therefore inferior to the Leica MZ7.

Does anyone have any suggestions on how I might judge the relative quality of
these two scopes, or does anyone have experience with either scope that might
shed light on this problem? Is my only alternative to find and buy a suitable
test slide? If so, can anyone point me to a source?

Thanks very much,
Ed

Ed Bachmann
Odum Institute for Research in Social Science
Manning Hall CB 3355
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-3355
(919) 962-0512



From daemon Fri Jan 12 10:39:34 2001



From: DMoravits-at-swri.edu
Date: Fri, 12 Jan 2001 10:37:47 CST
Subject: LM--need operator's manual

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I am looking for some sort of operator's manual for a Zeiss Axioskop (am unsure
of the date of production, but assuming the '90's).

Thank you,

Don Moravits
Senior Technician
Southwest Research Institute
6220 Culebra Road
San Antonio, Texas 78238

Voice-210-522-2891
Fax-210-522-6220
E-Mail-dmoravits-at-swri.edu


From daemon Fri Jan 12 10:50:48 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 12 Jan 2001 16:47:47 +0000 (GMT)
Subject: Re: STEM

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Thearith,

Here are a couple of references you might find useful, from a specialist
on this kind of electron microscopy. I'll send the abstracts as
attachments separately.

Srnova-Sloufova I, Lednicky F, Gemperle A, et al.
Core-shell (Ag)Au bimetallic nanoparticles: Analysis of transmission
electron microscopy images
LANGMUIR 16: (25) 9928-9935 DEC 12 2000

Lednicky F, Coufalova E, Hromadkova J, et al.
Low-voltage TEM imaging of polymer blends
POLYMER

} "Thearith H. Ung" wrote:
} }
} } Dear Colleagues,
} }
} } Does anyone know any place that has a TEM with a dedicated STEM unit
} } attached. The reason for the question is that I have particles composed of
} } cores and shells (coated particles), both of which are different materials.
} } By TEM I am not able to discern the shells from the cores because the
} } contrast is extremely low. However, I am told that a dedicated STEM unit
} } might allow me distinguish the shells from the cores based on the fact that
} } they both have very different z-contrast.
} }
} } Regards,
} } Thearith
} }
} } _________________________
} }
} } Thearith Ung, Ph.D.
} } Quantum Dot Corporation
} } 26136 Research Road
} } Hayward CA 94545
} } Tel: (510)-887-8775 (x4125)
} } Fax:(510)-783-9729
} } www.qdots.com

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+



From daemon Fri Jan 12 10:56:24 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 12 Jan 2001 16:53:10 +0000 (GMT)
Subject: Microscopy of core-shell: abstracts

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Thearith,

(I accidentally deleted the original message with your e-mail address)

Abstracts after the signature.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

Record 1 of 2

Author(s): Srnova-Sloufova I; Lednicky F; Gemperle A; Gemperlova J
Title: Core-shell (Ag)Au bimetallic nanoparticles: Analysis of
transmission electron microscopy images
Source: LANGMUIR 2000, Vol 16, Iss 25, pp 9928-9935
Addresses: Srnova-Sloufova I, Charles Univ, Dept Phys & Macromol Chem,
Hlavova 2030, Prague 12840 2, Czech Republic.
Charles Univ, Dept Phys & Macromol Chem, Prague 12840 2, Czech Republic.
Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague 6, Czech
Republic.
Acad Sci Czech Republ, Inst Phys, Prague 18221 8, Czech Republic.

Abstract: Layered core-shell bimetallic silver-gold colloids in the size
range of 10-16 nm have been prepared by the seed-growth method. Silver
nuclei were covered by gold shells of various thicknesses without any
stabilization agent. Interfacial (Ag)Au colloid-2,2'-bipyridine films were
prepared from these bimetallic colloids and used for the purpose of
analysis of transmission electron microscopy (TEM) images and electron
diffraction. Both observed and calculated TEM images were used to
characterize the prepared nanoparticles. On the basis of the analysis of
TEM images, the calculated TEM image contrast, and results obtained by
electron diffraction, energy-dispersive X-ray analysis, and other
experiments, the core-shell structure of the prepared (Ag)Au nanoparticles
was revealed. Particles were found to consist of a silver core and a gold
shell enriched with silver.


--------------------------------------------------------------------------------

Record 2 of 2

Author(s): Lednicky F; Coufalova E; Hromadkova J; Delong A; Kolarik V
Title: Low-voltage TEM imaging of polymer blends
Source: POLYMER 2000, Vol 41, Iss 13, pp 4909-4914
Addresses: Lednicky F, Acad Sci Czech Republ, Inst Macromol Chem,
Heyrovsky Sq 2, CR-16206 Prague 6, Czech Republic.
Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague 6, Czech
Republic.
Delong Instruments, Brno 61200, Czech Republic.

Abstract: Low-voltage transmission electron microscopy (LV-TEM) was
applied to obtain images of the phase structure of selected polymer blends
without any prior staining. The instrument used (LVTEM-5, working at 5 kV)
is of a novel construction combining visual-light and
electronmicroscopical techniques, resulting in an enhanced efficiency of
light transport to the eye and facilitating CCD imaging. Results were
compared wi th LV-STEM at 25 kV. Phase structure of polycarbonate/poly(
styrene-co-acrylonitrile) (PC/SAN), polystyrene/polypropylene (PS/PP), and
polyethylene/polypropylene blends (PE/PP, ADFLEX) were selected to
demonstrate the above techniques. The difference in density between the
individual components of polymer blends was found to be the reason for the
obtained image contrast. Differences less than 0.04 g/cm(3) can be traced
with this technique.


--------------------------------------------------------------------------------





From daemon Fri Jan 12 11:05:21 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 12 Jan 2001 09:00:28 -0800
Subject: Re: Using a microscope as a "flatbed" scanner

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First off, I can't understand the rationale for using an
LM to do neg scanning. Why not just use a scanner?
Easy, fast, reproducable.

If you do want to use an LM, it seems to me that a low
power objective is required in order to avoid imaging
mostly the grain structure. With a typical field of view
for the camera, you will likely be taking about 100
individual shots and then stitching them together.
A 2.5X objective works on low NA condensers. A
1X objective requires an ultra low condenser. But
even this would result in a 10X FOV on each image
frame.

Notwithstanding the above, if you do want to temporarily
mount 35mm frames, get Bair mounts from the Stock
Solution, Salt Lake City.

http://www.tssphoto.com/sp/index.html

These are self-adhesive cardboard mounts which stick
to themselves but not to the film. When you are done,
just peel the mount apart and your neg will fall out.

gary g.

http://photoweb.net



At 11:29 AM 1/11/01, you wrote:

} Hi! I am interested in using a light
} microscope to digitize photographic film, i.e.
} to use my light microscope as a film scanner
} to scan negatives.
}
} Since our lab does a fair amount of investigation
} into telepathology applications, we have scopes
} that are set up for the automatic scanning of
} multiple fields, merging of fields, etc. That
} problem is (relatively) solved.
}
} What I am getting stumped on here is how to
} mount my 35mm photographic negatives. If I
} just slap one on a slide and set a coverslip
} on top, I get (not surprisingly) nontrivial
} refractive problems with irregularities in the
} surface of the film.
}
} Unfortunately, these are photographic negatives
} which might have forensic/evidentiary value, so
} I can't dribble on any of the mounting media I
} can think of -- since I can't permanently mount
} the negative. I have to be able to take that
} negative at a later time and make an "original"
} photograph.
}
} Anybody have experience with this? Any lore
} would be appreciated.
}
}
} billo



From daemon Fri Jan 12 11:07:47 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 12 Jan 2001 17:05:00 +0000 (GMT)
Subject: Re: Ask-A-Microscopist: LM: Crystal Growth

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Dear Leonard,

Some things don't need solvents. For example, citric acid can be melted
on a hotplate at about 100^C, and if cooled slowly will give chunky
crystals in all sorts of birefringence colours.

If you have a hot enough plate, it's good to look at sodium nitrate (mp
about 305^C. This will form large slabs of crystals. These don't look so
good in themselves, but if you have a Bertrand lens or else simply pull
out the eyepiece and look at down the tube, many of them will display
concentric rings on a Maltese cross, the interference pattern resulting
from different path lengths of the two polarized rays through the crystal.
Try this with different magnification objectives. The citric acid will
also give wonderful interference patterns, but they're much more
complicated.

Other organic compounds can simply be melted, and when cooled between
cover slip and hot plate will form spherulites.

If you melt a thin film of polypropylene between slide and cover slip, and
cool slowly, you will get fantastic spherulites. Spherulites are found in
many plastics, but polypropylene is the star performer. (Further details
on request).

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

On Fri, 5 Jan 2001 us004118-at-mindspring.com wrote:

} Question: I am enjoying doing photomicrographs of crystal preparations
} in polarized light. However I have insuffient knowledge of chemistry to
} choose solvents without a long series of trial and error efforts.Can you
} give me a rationale and/or a reference to go about this in a more
} productive manner?

Email: us004118-at-mindspring.com
Name: Leonard Lessin, FBPA

School: (Retired Science & Medical Photographer)

State: NY

Zip: 10012







From daemon Fri Jan 12 11:22:10 2001



From: Joseph Passero :      jp-at-spacelab.net
Date: Fri, 12 Jan 2001 12:18:44 -0500
Subject: [LM - Stereo] AO Questions

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What is the difference in AO Stereo eyepieces between the 10X WF number 134 and the 10x WF
number 148?

Who (after market or used) has part for AO boom stand's number 562?

I am looking for rack and pinion set for the 562 stand?

Any one have a copy of service manual, instruction manual or catalog for a AO Stereo Zoom
model 580 pod?

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net

Shop online without a credit card
http://www.rocketcash.com
RocketCash, a NetZero subsidiary


From daemon Fri Jan 12 11:37:17 2001



From: E.M.M. Manders :      e.manders-at-chem.uva.nl
Date: Fri, 12 Jan 2001 18:28:28 +0100
Subject: FOCUS ON MICROSCOPY 2001, Amsterdam, April 1-4

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***************************************************
Last call for papers: FOCUS ON MICROSCOPY 2001
***************************************************

FOM 2001 , Amsterdam The Netherlands, April 1-4, 2001


FOM 2001 is a world leading international conference on advanced
microscopy. It is the joint meeting of the 13th International Conference on
Confocal Microscopy and the 13th International Conference on 3D Image
Processing in Microscopy.

FOM 2001 will be held at the Academic Medical Centre (AMC) of the
University of Amsterdam, Amsterdam, The Netherlands.

For more information visit: http://www.focusonmicroscopy.org

Call for abstracts
------------------
Deadline for abstract submission: february 1st, 2001.


Core conference subjects:
"INSTRUMENTATION" and "IMAGE PROCESSING AND ANALYSIS"

SPEAKERS: S. Hell (Gottingen, Germany), T. Wilson (Oxford, UK), S. Kawata
(Osaka, Japan) C.J.R. Sheppard (Sydney, Astralia), C. Coggswell (Sydney,
Astralia) E. Stelzer (Heidelberg, Germany) C. Cremer (Heidelberg, Germany),
P.A. Benedetti (Pisa, Italy), A. Kriete (Giessen, Germany), H. van der
Voort (Hilversum, The Netherlands), P.C. Cheng (Buffalo, USA), G.J.
Brakenhoff (Amsterdam, The Netherlands)


Special conference subjects this year:
MULTI-PHOTON MICROSCOPY" and "MICROSCOPY OF LIVING CELLS AND TISSUE".

SPEAKERS: A. Zumbusch (Muenchen, Germany), L. Moreaux (Paris, France) A.
Diaspro (Genova, Italy), M. Muller (Amsterdam, The Netherlands), K.
Sullivan (La Jolla, USA, to be confirmed), R. van Driel (Amsterdam, The
Netherlands), R. Eils (Heidelberg, Germany), T. Mistelli (Bethesda, USA; to
be confirmed), D. Gadella (Wageningen, The Netherlands), J. Dobrucki
(Krakov, Poland), A. Houtsmuller (Rotterdam, The Netherlands), E. Manders
(Amsterdam, The Netherlands)


Conference Office:
------------------
Mrs M.P.A. Beunk-Timmers
Nicolaes Tulp Institute
PO-Box 23213
1100 DS Amsterdam
The Netherlands
Fax: +31-(0)20-6963228
Phone +31-(0)20-5668585
Web: http://www.FocusOnMicroscopy.org
E-mail: info-at-FocusOnMicroscopy.org


Local Organising Committee:
---------------------------
Prof. G.J. Brakenhoff and Dr. E.M.M. Manders






---------------
Erik M.M. Manders, PhD
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam

Visit: Kruislaan 316, Building III, room 2.07, Amsterdam, The Netherlands
Plantage Muidergracht 12, 4th floor, Amsterdam, The Netherlands
Mail: Kruislaan 316
1098 SM AMSTERDAM
The Netherlands
E-mail: e.manders-at-chem.uva.nl
Tel: +31-(0)20-5256225 (5257702)(5255136)
Fax: +31-(0)20-5256271
Web: http://wwwmc.bio.uva.nl/
http://www.FocusOnMicroscopy.org/
---------------



From daemon Fri Jan 12 12:07:45 2001



From: William R. Oliver :      oliver-at-cpt.afip.org
Date: Fri, 12 Jan 2001 10:40:23 -0500 (EST)
Subject: Re: Using a microscope as a "flatbed" scanner

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On Fri, 12 Jan 2001, Gary Gaugler wrote:

} First off, I can't understand the rationale for using an
} LM to do neg scanning. Why not just use a scanner?
} Easy, fast, reproducable.

There are a couple. The driving reason for me is that this gives me a
*lot* more control in exploiting the lighting of the negative.

I do forensic image processing as well as "regular" pathology stuff.
Consider the following scenario:

A bad guy is sitting in a shadowed room aiming a rifle through a
window. Joe Snuffy tourist takes a photo of the street which
incidentally captures the window (let's assume that Joe Snuffy
is a purist and isn't using a Mavica, in which this is all moot
and useless). The window is largely a dark blob.

The task, then is to get as much information of that dark blobby window
as possible. If you look at most film scanners on the market,
particularly in any reasonable price range, you don't have the option
of cranking your illumination up or down, of sticking filters in the
light pathway, of doing all the stuff we take for granted as
microscopists.

There's a lot of tools in them thar microscopy hills. It ain't so, it
seems, with the film scanners I have available.

Instead, almost always, the only thing you can play with is the gamma.
That means you are going to spend a lot of your representation space on
stuff you don't want to see. The scanner is going to get two bits of
data out of that blob, whatever knob you twiddle.

Playing games resetting the gamma as an option in a TWAIN driver won't
fix this, and I haven't seen a "burn the bejezus out of this little
area here and ignore the rest of the slide" button. Oh, sure, you can
take those two bits the scanner gives you and display it as 0 and 1 or
0 and 255, but it ain't going to fill up the space in between. Not
even with noise. The bottom line is that my impression is that film
has dynamic range that is not accessible to most film scanners.

As a secondary issue, I have a few ideas for for playing with grain and
restoration that might benefit from data aquisition at a higher
resolution.


}
} If you do want to use an LM, it seems to me that a low
} power objective is required in order to avoid imaging
} mostly the grain structure.

Sorta, but I am actually interested in seeing about characterizing the
grain structure and seeing if that characterization can be useful in
restoration.

Finally, I have a good characterization of the mtf of my microscope. I
don't have a good characterization of the mtf of my flatbed scanner.
While that doesn't help me with blind deconvolution for the camera mtf,
it does help me for at least that part of the restoration of the
image. I may be goofy, but I'd like to play with it a little.

If you have some experience with this, I'd love to learn from you. I
figure that *somebody* must have played with this, but I haven't been
able to find much literature on it. Do you have some lore you would
like to share (nudge, nudge)?


} With a typical field of view
} for the camera, you will likely be taking about 100
} individual shots and then stitching them together.

Something like that. But that stitching and data handling
is something we do on a daily basis.

}
} Notwithstanding the above, if you do want to temporarily
} mount 35mm frames, get Bair mounts from the Stock
} Solution, Salt Lake City.
}
} http://www.tssphoto.com/sp/index.html


Thanks!


billo



From daemon Fri Jan 12 12:52:38 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 12 Jan 2001 08:49:15 -1000 (HST)
Subject: Re: STEM

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} Does anyone know any place that has a TEM with a dedicated STEM unit
} attached. The reason for the question is that I have particles composed
} of cores and shells (coated particles), both of which are different
} materials.
} By TEM I am not able to discern the shells from the cores because the
} contrast is extremely low. However, I am told that a dedicated STEM unit
} might allow me distinguish the shells from the cores based on the fact
} that they both have very different z-contrast.

I agree with the person who suggested an energy-filtering TEM, such as the
Zeiss 902. We have the newer version, the LEO 912, and I have also been
doing some work with coated nanoparticles. Yes, it is possible to see the
difference between the cores and coating with this type of instrument.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Fri Jan 12 13:06:30 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 12 Jan 2001 11:03:17 -0800
Subject: Gold staining heparin

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Hi:

Its me again.

Someone here would like to probe for heparin on some amyloid fibrils they
have made. Fibril formation is different in the presence of heparin and
they are curious to know if the heparin is binding to the fibrils or not.

They prepare the fibrils in their lab, don't ask me how, and apply liquid
suspensions to a formvar coated grid. We negative stain with UA and then go
to the TEM to look for fibrils.

The results of the neg stain search can be variable, sometimes the fibrils
look like fibers, other times it seems to be just a bunch of junk, so the
fibril formation is not always we defined. These folks are trying to figure
out whether to probe the fibrils on the grid, or before while still
suspended. But they tell me that often the fibrils do not survive too many
washings in suspension, so maybe probing in solution before applying to
grids could be a problem.

Any ideas, this is not my area of expertise.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Jan 12 14:28:17 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Fri, 12 Jan 2001 15:22:27 -0500
Subject: Food Structure and Functionality Symposium 2001

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Food Structure & Functionality Symposium 2001
An international symposium leading food structure & Functionality studies through the 21st century
*webaddress at the AOCS site http://www.aocs.org/member/division/fsff/index.htm*

Being held in conjunction with the , May 13-16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e-mail us at: meetings-at-aocs.org

The symposium has two themes:
* New and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* Food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting.

Tentative Program (confirmed as of January 12th, 2001)

May 13th - Short Courses (short courses will run for a full day, and will run concurrently)

1) Food Contaminants. Contact person - Mark Auty
2) Specific Labeling in Foods. Contact person - Marcel Paques

May 14th-16th inclusive - Technical sessions
6 sessions will run over 3 days

Morning
Symposium opening
Plenary lecture - Food Quality andwhy the Structure matters. P. Lillford, Unilever Research, Colworth House UK

Session 1: Dairy Products and Fat Based Foods Session - chairs M. Auty and M. Paques

Milk protein polysaccharide interactions in aqueous solutions and oil-in-water emulsions.
H. Singh*, Y. Hemar & P. Munro, Institute of Food, Nutrition and Human Health
Massey University, Palmerston North, New Zealand

Structural functions of dairy ingredients in products formulated with taro flour.
C. Onwulata, USDA/ARS,Eastern Regional Research Center,Wyndmoor, PA 19038

Confocal imaging of galactomannan mode of action in recrystallisation of ice in model ice cream
D. Ferdinando, Unilever Research Colworth House, UK

Heating of Food Proteins in a Closed System at High Temperature
N. Kitabatake, Kyoto University, Japan

Milk Protein - molecular components and functional properties. N. C. Ganguli, Indian Dairy Association

Afternoon
Session 2: Food Safety - chairs J. W. Arnold and R. Droleskey
Prevention of Foodborne Illness Through Sanitation and Processing Technology
J.W. Arnold; USDA/ARS, Russell Research Center; Athens, GA 30604

PreHarvest Intervention Strategies to Control Foodborne Pathogens in Poultry
J. A. Byrd; USDA/ARS/SPARC; College Station, TX 77845

Interactions of Competitive Exclusion Cultures with the Intestinal Mucosa of Newly Hatched Chicks
R. Droleskey; USDA/ARS/SPARC; College Station, TX 77845

Adhesion of Salmonella on Alfalfa Sprouts
A. Chartowski; USDA/ARS, Western Regional Research Center; Albany, CA 94710

Growth of Fusarium moniliforme Dependent upon Corn Tissue Type
I. E. Yates; USDA/ARS, Russell Research Center; Athens, GA 30604

Dedicated poster viewing 4:00-6:00PM

Evening: Round Table Discussion - topic to be announced


Tuesday, May 15th, 2001
Morning
Session 3: New Methods and Techniques for Food Structure and Functionality Analysis -chair K.Groves

Diffusing wave spectroscopy - a new and non-invasive method for the investigation of the structure, dynamics and interactions in complex food systems
M. Alexander* and P. Schurtenberger

Food: How complex can it be?
E. Esselink ,Unilever Research Vlaardingen, The Netherlands

Immunolocalization of Transgenic Protein in Wheat Endosperm
M. L. Parker *, E. Stoger, R. Casey and P. Christou, Institute of Food Research, Norwich, England.

Structure/Function relationships through microrheology.
M. Paques, Wageningen Centre for Food Sciences/Unilever Research Vlaardingen

Specific labeling techniques for foods
J. Leunissen, Aurion: Immuno-Gold Reagents & Accessories, The Netherlands


Food Structure and Functionality Division Luncheon. Dr. Brian Brooker (Institute of Food Research, England * retired) will be presented with the Food Structure and Functionality Division award and will give a presentation entitled: Fat crystals - the importance of
being small.

Afternoon
Session 4: Agricultural Applications of Microscopy and Imaging Session- chairs D.F.Wood and P. Allan-Wojtas

The Utility of Sorting in Agriculture.
H. J. Arnott, Department of Biology, University of Texas at Arlington, Texas

The Potential for Automatic X-ray Sorting of Insect Infested Grain
R. Haff . USDA - ARS - WRRC, Albany, California.

Automated Sorting of Almonds with Embedded Shell by Laser Transmittance Imaging
T. Pearson* R. Young, USDA - ARS - WRRC, Albany, California.

Use of a GFP-transformed strain of Fusarium graminearum to study ear rot development in corn
S. S. Miller. , AAFC - ECORC, Ottawa, Ontario, Canada.

Popping modifies endosperm structure and improves digestibility in maize and sorghum.
M.L. Parker, Institute of Food Research, Norwich, England.

Microstructural Changes in Rice During Cooking
D. Wood* and P.C. Yu . USDA - ARS - WRRC, Albany, California.

Evening: Food Structure and Functionality Division Member meeting

Wednesday, May 16th, 2001
Morning
Session 5: Ingredients and Food Processing - chairs D. Kittleson and J. Charbonneau

Water continuous fat crystal networks in ice cream induced by unsaturated monoglycerides
N. M. Barfod , Danisco Cultor, Brabrand, Denmark

High pressure application fo food systems and its impact on functional ingredients
B. Tauscher*, P. Butz,and A.F. Garcia, Federal Research Center for Nutrition, Karlsruhe, Germany

Specificity and application of lipolytic enzymes in bread making processes
T.B. Frandsen, T. Spendler, G. Budolfsen, L. Christiansen and J. B. Neilsen, Novozymes A/S, Bagsvaerd, Denmark

Afternoon
Session 6: Colloidal and Interfacial Sciences - chairs D. Pechak and M. Paques

Rheology and Structure of Particulate Protein Gels
T. van Vliet, Wageningen Centre for Food Sciences,Wageningen, The Netherlands

Posters:

1. In situ study of the effect of heating on dough components using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR)

O. Sevenou, S.E. Hill, I.A. Farhat and J.R. Mitchell. Division of Food Sciences, University of Nottingham, Loughborough, UK

2. Antioxidative activity of the crude extract of lignan glycosides from sesame meal

Y-S Shue and L.S. Hwang, Graduate Institute of Food Science and Technology, National Taiwan University, Taiwan, R.O.C.

3. Near Field Microscopy of phase separation in a mixed interfacial protein/surfactant film

A. P. Gunning, A.R. Mackie, A.R. Kirby and V.J. Morris, Institute of Food Research, Norwich Research Park, Norwich, UK

Contact information for the chairs is shown below, in alphabetical order:

Paula Allan-Wojtas
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre, Kentville, Nova Scotia, Canada
B4N 1J5
Tel: (902) 679-5566
FAX: (902) 679-2311
email: allanwojtasp-at-em.agr.ca

Judy Arnold
USDA -ARS - RRC
950 College Station Rd.
P.O. Box 5677
Athens, GA 30604-5677
USA
Tel: (706) 546-3515
FAX: (706) 546-3068
email: jarnold-at-ars.usda.gov

Mark Auty
Dairy Products Research Centre
TEAGASC
Moorepark, Femoy, Co. Cork
Ireland
Tel: 011-353-25-42447
FAX: 011-353-25-42340
email: mauty-at-moorepark.teagasc.ie

James E. Charbonneau
National Food Processors Association
Food Chemistry and Packaging Department
1401 New York Ave, NW
Washington, D.C. 20005
USA
Tel: (202) 639-5972
FAX: (202) 639-5991
email: jcharbo-at-nfpa-food.org

Kathy Groves
Leatherhead Food Research Association
Randalls Road, Leatherhead
Surrey KT22 7RY
England
Tel: 44 0132 822330
FAX: 44 0132 386228
email: kgroves-at-lfra.co.uk

Diana Kittleson
Pillsbury TPC Labs
737 Pelham Blvd.
St. Paul, MN 55114
USA
Tel: (651) 917-5859
FAX: (651) 917 5850
email: dkittleson-at-pillsbury.com

Tony McKenna
New Zealand Dairy Institute
Private Bag 11 029
Palmerston North,
New Zealand
Tel: 011 64 6 350 4649
FAX: 011 64 6 356 1476
email: tony.mckenna-at-nzdri.org.nz

Marcel Paques
Wageningen Centre for Food Sciences/Unilever Research Vlaardingen
P.O. Box 20, 6710 BA Ede
The Netherlands
Tel: 011 31 318 659690
FAX: 011-31-318 650400
email: paques-at-nizo.nl

David Pechak
Kraft Technology Centre
801 Waukegan Road
Glenview, IL 60025
USA
Tel: (847) 646-4808
FAX: (847) 646-3864
email: dpechak-at-kraft.com

Delilah Wood
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5653
FAX: (510) 559-5777
email: wood-at-pw.usda.gov



From daemon Fri Jan 12 15:12:21 2001



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Fri, 12 Jan 2001 14:47:28 -0600
Subject: single cell, Lanthanum and Ruthenium

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Greetings,
Does anyone have experience with lanthanum and/or ruthenium HT staining specifically for isolated cells??
I prepared two samples of single atrial cells, using protocols from the 60's and 70's. The ultrastructure was great, in part because I believe that the lanthanum as well as the ruthenium enhanced the UA and LC staining. I was hoping for a much more dense cell margin that would help to delineate vesicles that might be open to the surface.
I know that Ruthenium HT is supposed to bind to the glycocalyx and stain well....but the lanthanum protocols I could find were all intact tissue where the spaces between cells were filled with dense material. I am not sure that lanthanum works for single cells, but gave it a try. Ruth. HT was added to both the GA and OsO4, but lanthamun was used, per protocol, only in the OsO4. Etoh dehydration and Embed 812 resin.
Any sage wisdom on how to improve staining on a single cell prep would be helpful.
Linda Fox
lfox1-at-lumc.edu


From daemon Fri Jan 12 15:31:19 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 12 Jan 2001 16:27:35 -0500
Subject: Facility management topics

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fellow Microscopists:
As many of you know, the Facility Management session held at M&M2000 was very well received and there was a unanimous request for it to be continued. Therefore a similar session will be held at M&M 2001. I must finalize the topics within the next week or two. Since the purpose of this session is to discuss topics of interest to facility managers, I would like your input before the session is finalized.

The topics covered in the M&M 2000 session with transcripts published in Microscopy Today were:
1) Multi-user Facilities...managing users
2) Justification of Costs, cost recovery, electronic bookkeeping and billing
3) Equipment Maintenance: Vender service contracts vs. Independent providers vs. Insurance company/self-insure options.

The following are some suggestions that were gleaned from the evaluation forms and discussion during the session.

1) Training Users…courses, workshops, …what works for whom?
2) Scientific Ethics…What are our responsibilities as lab Managers?
3) Mission statements/lab organization/lab business plans/future planning issues
4) Staffing issues including training, part-time student help, Training course T.A’s
5) Daily issues…prioritizing use, prioritizing service projects, dealing with difficult users/customers
6) Legal issues involving data ownership, confidentiality of data, handling of potential evidence.
7) Justification for new equipment
8) Commercial use of university facilities
9) Microscopy as a research tool…integration of industry or campus facilities in coordination with other core facilities.
10) Issues unique to Multi-user and/or Service facilities and how such facilities can coexist.


The first 2 received the greatest number of requests. However there may be more relevant topics now. Please rank the 3 of highest priority to you or suggest additional topics of interest. I will sift through all replies at the end of next week and narrow them down to 3. Also please feel free to supply the names of individuals to lead specific discussion topics.
The format will be the same as the last session. A presenter will give a 10-15 min. introduction of the topic followed by ~30 min. open discussion.

Thanks,
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



From daemon Fri Jan 12 15:32:48 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 12 Jan 2001 16:33:54 -0500
Subject: Re: Microtome injury

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Hi All,
On the "serious" side of this, and to make the safety police happy,
EMS sells a plastic guard that holds razor blades, exposing just a
small expanse for trimming. I had bought some years ago, on the
"that sound good" premise. Personally, I found them awkward to use,
but they are cheap, and having them in the lab (even if not used) may
be enough to satisfy your safety wizards.

Disclaimer: I have no financial interests in EMS.

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Jan 12 17:24:00 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Fri, 12 Jan 2001 17:18:04 -0600
Subject: mounting diatoms for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mounting of particles on the order of 50-100um onto double stick tape is
easily done as previously noted by using a very fine artist's brush and
distilled water. But this should be done moist, rather than wet. After
dipping the brush into DW, twirling the length of the brush end on a lab
tissue both removes excess water and creates a finer point at the tip. The
moist, fine-tipped brush can then not only pick up the diatom, foram or
other particle, but can also be used to orient it and position it on the
tape with good control. This method also prevents glue from bleeding up the
particle. Particles of this size range can also be set down on a thin
layer of silver conducting paint that's still wet, if you can chase the
drying horizon fast enough, or on a thin layer of still-wet collodion (the
same adhesive used by belly dancers to secure the navel jewel, but that's
another story).


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Fri Jan 12 17:24:01 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 12 Jan 2001 17:20:03 -0600
Subject: RE: Nikon SMZ800 vs Leica MZ7 Stereozooms

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Ed:
Edmund Scientific sells test slides for microscopes. Perhaps you can
find an adequate one there. Some test slides run up $300, though. Or, what
about using diatoms to compare one scope with another?

Sam Purdy
Technical Center/National Steel Corp
Trenton MI
} ----------
} From: ed_bachmann-at-unc.edu
} Sent: Friday, January 2001, 11:01 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Nikon SMZ800 vs Leica MZ7 Stereozooms
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am trying to judge the optical quality of two similar stereoscopic zoom
} scopes: the Nikon SMZ800 and the Leica MZ7, both equipped with a 1x
} Planapochromatic objective.
}
} Leica publishes resolution figures for the MZ7, claiming 309 line pairs
} per mm.
} Nikon does not, and I haven't been able to persuade the Nikon
} representative to
} "characterize" the resolution of the SMZ800, although he says he can. I
} do not
} have the necessary test slide, nor do I know where I can get one with a
} scale
} graduated from say 200-400 lp/mm. I do know though that such a slide
} would cost
} probably $300-$500.
}
} Interestingly, Nikon publishes a resolution number for its next higher
} quality
} scope, the SMZ1000. It claims the SMZ1000 can resolve 300 lp/mm with a 1x
} Planapo. Because Nikon product literature doesn't publish a resolution
} for the
} SMZ800, I suspect that its resolution is substantially lower than 300
} lp/mm, and
} therefore inferior to the Leica MZ7.
}
} Does anyone have any suggestions on how I might judge the relative quality
} of
} these two scopes, or does anyone have experience with either scope that
} might
} shed light on this problem? Is my only alternative to find and buy a
} suitable
} test slide? If so, can anyone point me to a source?
}
} Thanks very much,
} Ed
}
} Ed Bachmann
} Odum Institute for Research in Social Science
} Manning Hall CB 3355
} University of North Carolina at Chapel Hill
} Chapel Hill, NC 27599-3355
} (919) 962-0512
}
}




From daemon Fri Jan 12 20:58:08 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sat, 13 Jan 2001 12:49:33 +1000
Subject: RE: mounting diatoms for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe that the time-honoured method of manipulating small particles with a
fine brush can be improved on. A customer thought that certain specimen should
not be wetted, because they were to be weighed. Presumably they could be left
to dry, but that was inconvenient.
I suggested one of those vacuum pick-up devices; these pick up specimens using
a square tipped syringe needle. Obviously those needles are too large for
foramifera but they can be tipped with thin plastic tubing. On the first try I
pulled some 0.3mm internal tubing using a PE transfer pipette. With practice
smaller tubes could be produced. I suggest that manipulating specimens with a
vacuum pick-up is faster and more precise than using a brush.
I have used a vacuum pick-up for placing (and turning over) TEM grids when
making support films. Its a rather more elegant and faster method than using
tweezers.
Disclaimer: ProSciTech is a supplier of vacuum pick-ups.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, January 13, 2001 9:18 AM, Dee Breger
[SMTP:micro-at-ldeo.columbia.edu] wrote:
}
}
} Mounting of particles on the order of 50-100um onto double stick tape is
} easily done as previously noted by using a very fine artist's brush and
} distilled water. But this should be done moist, rather than wet. After
} dipping the brush into DW, twirling the length of the brush end on a lab
} tissue both removes excess water and creates a finer point at the tip. The
} moist, fine-tipped brush can then not only pick up the diatom, foram or
} other particle, but can also be used to orient it and position it on the
} tape with good control. This method also prevents glue from bleeding up the
} particle. Particles of this size range can also be set down on a thin
} layer of silver conducting paint that's still wet, if you can chase the
} drying horizon fast enough, or on a thin layer of still-wet collodion (the
} same adhesive used by belly dancers to secure the navel jewel, but that's
} another story).
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 914/365-8640
} F: 914/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)
}
}



From daemon Sat Jan 13 06:30:15 2001



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Sat, 13 Jan 2001 12:24:08 -0000
Subject: Retirement

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
I've been thrown on the scrapheap at 60. So I'll be unsubscribing soon but
will keep an eye on the list over a colleague's shoulder. Many thanks for
all the help and interesting items, especially the forensic stuff - a
fascinating and useful insight into the problems of others.
Byeeee, Chris, Plant Path., IACR-Rothamsted, UK. chris.smith-at-bbsrc.ac.uk


From daemon Sat Jan 13 12:10:35 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Sat, 13 Jan 2001 18:06:22 -0000
Subject: Microtome safety

Contents Retrieved from Microscopy Listserver Archives
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Most injuries while trimming blocks with hand-held razor blades result
either from the free
hand not knowing where the razor hand is, or from sudden uncontrolled
movement of the blade when force is released at end of cut, or due to
slippage, etc.
when the razor hand / arm movement is unlimited.
A couple of simple rules will prevent these situations from arising.
1) Look before you move
2) when cutting, use both hands - hold one end of the blade in each
hand at all times. That way you know where both hands are in relation
to the blade all the time
and there is also improved control over blade movement
3) Never cut with the blade at the end of an unsupported arm. Arms
and
hands should be supported as close to the specimen as possible, so
that
blade movement is strictly limited to the range that can be driven by
deliberate finger movements, and free, involuntary movements cannot
occur.

I recommend you not to wear gloves because they interfere with touch
perception and interfere with your grip on the blade. Has your safety
officer seen you in action? Does he really know what the risks are, or
is his imagination running riot? Show him your procedures.
(Any gender-specificity in the above is wholly unintentional)

Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility

Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401



From daemon Sat Jan 13 15:05:22 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 13 Jan 2001 13:00:06 -0800
Subject: Re: Retirement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hi Listers,
} I've been thrown on the scrapheap at 60. So I'll be unsubscribing soon but
} will keep an eye on the list over a colleague's shoulder. Many thanks for
} all the help and interesting items, especially the forensic stuff - a
} fascinating and useful insight into the problems of others.
} Byeeee, Chris, Plant Path., IACR-Rothamsted, UK. chris.smith-at-bbsrc.ac.uk

Chris -

WRONG attitude! I retired at the same age, and started Project MICRO so
that I could continue to share my love of the microworld. I suggest that
you contact the RMS equivalent, AMFES, and have some fun yourself!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Jan 15 08:48:34 2001



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Mon, 15 Jan 2001 14:25:12 -0000
Subject: Resurrection

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
Many thanks indeed for the unanticipated response to my 'for info' note.
Attitude adjustment assimilated. The world will hear from me again! ttfn,
Chris


From daemon Mon Jan 15 11:44:55 2001



From: TEDPELLA-at-aol.com
Date: Mon, 15 Jan 2001 12:37:52 EST
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ted Pella


From daemon Mon Jan 15 11:52:52 2001



From: OCONNELL-at-ltu.edu
Date: Mon, 15 Jan 2001 12:50:00 -0500 (EST)
Subject: Job posting--revisited

Contents Retrieved from Microscopy Listserver Archives
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Still looking...for a few good account representatives for an
electron and optical microscopy service. territory is open.,
excellent earning potential. Please call Dick O'Connellat
734-668-3309 or e-mail Oconnell-at-ltu.edu. for more information.
(I was unable to reach some of you who responded because of
one reason or another--you know who you are. Please contact
me again with a phone number or some other means to reach you.)

Thanks
Dick O'Connell


From daemon Mon Jan 15 18:24:41 2001



From: RangeTS-at-aol.com
Date: Mon, 15 Jan 2001 19:18:08 EST
Subject: Job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for someone with EDS/SEM experience to work out of our Florida
office. Responsible for setting up procedures and repairing systems in house
as well as customer sites. Working without supervision is a must. if you
are interested to know more about the position, please send your
qualification and salary history to our Email address. rangets-at-aol.com



From daemon Mon Jan 15 20:41:55 2001



From: Boron nitride :      boronnitride-at-hotmail.com
Date: Mon, 15 Jan 2001 20:35:27 -0600
Subject: SOFTWARE FOR CREATE ATOMIC STRUCTURES OF INTERFACE OR DEFECT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleage,
Does anybody know if there is a software that can create
the atomic structure for an interface or a dislocation, so that an image
simulation (by using EMS or Mac Tempas) can be carried out directly for such
structures? I know crystal Kit can create an interface structure,however,
its atomic cordinations can not be exported directly to MacTempas or EMS
directly for image simulations.

I would be greatly appreciate if you could provide such kind of
information!

Sincerely,


James

_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com




From daemon Mon Jan 15 22:07:37 2001



From: Rosalie Daniel :      rosalie-at-deakin.edu.au
Date: Tue, 16 Jan 2001 14:59:03 +1100
Subject: Staining Spurr's and LR sections for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I a trying to stain sections of plant root that has been infected with a
fungus to highlight certain features including lignin, callose, suberin and
the fungal tissue itself. However, my samples have been embedded in LR
White and Spurr's Resins. I am having difficulty finding protocols for
staining of these resins. Most protocols refer to paraffin sections and it
is not possible to use ethanol based stains for these resin sections. Does
anyone know of any good references, books etc where I can find protocols
for staining of these resins for LM?
Thanks for your help.
Rose




From daemon Tue Jan 16 05:38:39 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Tue, 16 Jan 2001 21:34:39 +1000
Subject: RE: Staining Spurr's and LR sections for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several stains are used with resin embedded sections. The favorite seems to be
Toluidine blue. This topic was discussed last March and several postings will
be in the Archives. The differentiation method I contributed then, but to make
this posting useful, I'll give here "my" complete method. There are many
variants, most recently contributors to the histoserver were "frothing" how
wonderful the results of staining with a combination of urea and Toluidine blue
are - I have no experience with that.

Make up 0.5% Toluidine blue and 1% Borax in water. This will keep "forever" in
a stoppered bottle on the bench. Keep with it a small funnel and a double layer
filter paper.

Sections should be well adhering to a slide after heating on a 60-80 degree
hotplate for some minutes after the sections had dried. Run a thick felt-tip
pen around the section - on the underside. This will help locating sections and
will not wash off.


Poor about 5ml of stain into the filter and apply a couple of drops to the
sections. Sit the funnel back on the bottle recovering the stain.

Place the section on a hotplate and leave until the edge of the stain has dried
up.

Now a few drops of water could be added to gently remove the remaining stain.
This would result in a fairly intense, but blue-only staining.

Its better to differentiate.
This is one of the most attractive features of the Toluidine stain as it can
give a two
colour, pink and blue rendition. This shows cell features similar to a double
staining with Haematoxylin & Eosin.

After staining on a hotplate add a drop of acid alcohol and then rinse the
slide with distilled water. The acid alcohol can destain partially or even
completely, but a brief application brings forth the two colours.

Make acid alcohol by adding to 25ml of 50% Ethanol a couple of drops of 1N HCl.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, January 16, 2001 1:59 PM, Rosalie Daniel
[SMTP:rosalie-at-deakin.edu.au] wrote:
}
} Hello,
} I a trying to stain sections of plant root that has been infected with a
} fungus to highlight certain features including lignin, callose, suberin and
} the fungal tissue itself. However, my samples have been embedded in LR
} White and Spurr's Resins. I am having difficulty finding protocols for
} staining of these resins. Most protocols refer to paraffin sections and it
} is not possible to use ethanol based stains for these resin sections. Does
} anyone know of any good references, books etc where I can find protocols
} for staining of these resins for LM?
} Thanks for your help.
} Rose
}
}



From daemon Tue Jan 16 06:34:45 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 16 Jan 2001 04:31:26 -0800 (PST)
Subject: Re: Staining Spurr's and LR sections for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rose:

LR White is a methacrylate, and most routine paraffin stains can be used
directly with minor time modifications (the methacrylate can give some
background staining, but usually umimportant). Spurr's (and other epoxies)
can also be stained with differential methods--primarily using Toluidine
Blue or methylene blue. Check out Hayat's book on staining (MA Hayat,
"Stains and Cytochemical Methods", Plenum Press, 1993). He includes a broad
range of stains, including PAS, basic fuchsin-toluidine blue (similar to
H&E), Azure II-methylene blue-safranin O, H&E, and a thionin-acridine orange
(specifically for plant tissues). Another good source of references would
be to go to PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and do a search for
stains, then for resins or methacrylate, combine your searches, and use the
original papers (I know there are a lot out there). BTW, most suppliers of
stains (e.g. EM Sciences, Polysciences, Ted Pella, EB Sciences, Fullam,
Ladd, ProSciTech,SPI, etc) will have protocols for using their stains on LR
White and Spurr's--another good source for you. Hope this helps.

Roger C. Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

I have no financial interest in any of the mentioned vendors/suppliers (and
if I missed anyone--I apologize, it was purely a senior moment).

(On Tue, 16 Jan 2001 14:59:03 +1100, Rosalie Daniel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I a trying to stain sections of plant root that has been infected with a
} fungus to highlight certain features including lignin, callose, suberin
and
} the fungal tissue itself. However, my samples have been embedded in LR
} White and Spurr's Resins. I am having difficulty finding protocols for
} staining of these resins. Most protocols refer to paraffin sections and
it
} is not possible to use ethanol based stains for these resin sections.
Does
} anyone know of any good references, books etc where I can find protocols
} for staining of these resins for LM?
} Thanks for your help.
} Rose
}
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue Jan 16 07:39:16 2001



From: Carmen =?iso-8859-1?Q?Mart=EDn?= :      cmartal-at-terra.es (by way of Nestor J.
Date: Tue, 16 Jan 2001 07:33:53 -0600
Subject: Light Microscopy for vegetal tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I work with radicle tips and I'm looking for an effective stain in order
to reveal different cellular types such as xylem and/ or phloem, but I'm
havin some problemsa in finding protocols. Can anybody help me? Thanks
Carmen Martín




From daemon Tue Jan 16 07:56:46 2001



From: jshields-at-cb.uga.edu
Date: Tue, 16 Jan 2001 08:58:47 -0500
Subject: more microtome safety

Contents Retrieved from Microscopy Listserver Archives
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One of our students was worried and put labeling tape (sometimes
referred to as "time tape") along the edge to be held (we normally
break a double edge in half).
We also have a very graphic cartoon drawn by our grad student
depicting a severed finger complete with blood (he was getting
frustrated with the EM students...)

Regarding the safety officers - teach *them* to section...


John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu


From daemon Tue Jan 16 09:14:26 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 16 Jan 2001 10:08:25 -0500
Subject: Re: single cell, Lanthanum and Ruthenium

Contents Retrieved from Microscopy Listserver Archives
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Hi Linda,
I worked with isolated ventricular cells many years ago, and as I
remember, there was always some variablilty of staining intensities
between cells that were processed in the same sample. We used cell
that were isolated using enzymatic treatment in conjunction with
shaking ( I believe that was pretty standard. This was in the early
1980's, work with Beatrice Wittenberg and Thomas Robinson). Cells
that ended up in the same dish for study, and were fixed & processed
together, took the stains differently. We also used Ruthenium Red to
stain the glycocalyx as well as Alcian Blue, Safraninin O, phenylene
diamine and a few other stains. You can look up the papers by
Simionescu & Simionescu from the 1970's for examples.
Caveoli can also be visualized with careful staining of silver-grey
sections. You can also try bismuth instead of lead, but use it at
1/10 the recipe's strength and for a shorter time.

good luck,
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jan 16 12:00:43 2001



From: Joyce Craig :      j-craig-at-csu.edu
Date: Tue, 16 Jan 2001 11:47:46 -0600
Subject: job posting

Contents Retrieved from Microscopy Listserver Archives
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The Department of Biological Sciences at Chicago State University is
looking for an experienced electron microscopy technologist.
We teach a course in TEM in the fall and SEM in the spring. In the
summer we have students from several programs who learn about EM and
complete a simple research project.
We work on research projects with undergraduates, graduate students, and

faculty.
We have a really nice facility with JEOL 1200 STEM, JEOL 2100 SEM, and
RMC ultramicrotomes with cryo.
We have a beautiful campus that is very easy to get to as it is right at

several major freeways.
Our website will give you more information:
WWW.CSU.EDU/jbrown/EM



From daemon Tue Jan 16 14:39:10 2001



From: Michael D. Standing :      Michael_Standing-at-byu.edu
Date: Tue, 16 Jan 2001 13:37:55 -0700
Subject: Metal for sputter coating targets

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

In our lab we have been sputter coating with gold since the dawn of time.
This has essentially been the "traditional" metal we have used for coating
our non-conductive samples. We have found that in some of our applications
that the gold grain is to large and is causing us problems in visualizing
some of the smaller structures (primarily in engineering samples) that we
are interested in. We currently sputter our samples for 1 to 4 minutes with
a current reading of 18 to 20 mA using an argon atmosphere in the coater.
We are aware that chromium and/or osmium coating are preferential to gold
sputtering, but those technologies are not available to us at this time.
What I would like to know is:
1) What are the advantages of using something other than gold as the target
material (eg. Gold palladium, platinum palladium, or just platinum)?
2) Are there other materials that will work besides these metals that would
give us equal service for small grain and a stable coating?
3) Are there operating conditions we could try that would still give us good
coating but reduce the grain size of the film material?
Thanks for your help,

Mike

======================================
Michael D. Standing
BYU Microscopy Lab
401 WIDB
Brigham Young University
Provo, UT 84602

e-mail: Michael_Standing-at-byu.edu
phone: (801) 378-4011
fax: (801) 378-3937
======================================




From daemon Tue Jan 16 14:50:16 2001



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Tue, 16 Jan 2001 12:41:07 -0800
Subject: mounting supplies - bakelite ring forms

Contents Retrieved from Microscopy Listserver Archives
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Hello listers-
I am looking for a supplier of bakelite ring forms 1 inch in diameter
(outside). Would someone contact me with that information. I can't
find anything on the web.
I appreciate your help!
Many Thanks,
Sarah
--
Sarah A.W. Lundberg
Electron Microanalysis and Imaging Laboratory
Department of Geoscience, UNLV
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010

EPMA Lab (702) 895-2660
SEM Lab (702) 895-2462
Office (702) 895-1134
Fax (702) 895-4064
Dept. Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMIL.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635




From daemon Tue Jan 16 15:11:25 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Wed, 17 Jan 2001 08:07:46 +1100
Subject: Re: Metal for sputter coating targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Michael,
you dont say what resolution you need, but if you cut the current on your
existing gold system down to 4-5mA for 3 min or so, you may see a
considerable improvement in the 10-50,000X range.

Sally Stowe


Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218 or 6125 8525
http://www.anu.edu.au/EMU


} } } "Michael D. Standing" {Michael_Standing-at-byu.edu} 01/17/01 07:37am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers:

In our lab we have been sputter coating with gold since the dawn of time.
This has essentially been the "traditional" metal we have used for coating
our non-conductive samples. We have found that in some of our
applications
that the gold grain is to large and is causing us problems in visualizing
some of the smaller structures (primarily in engineering samples) that we
are interested in. We currently sputter our samples for 1 to 4 minutes
with
a current reading of 18 to 20 mA using an argon atmosphere in the coater.
We are aware that chromium and/or osmium coating are preferential to gold
sputtering, but those technologies are not available to us at this time.
What I would like to know is:
1) What are the advantages of using something other than gold as the
target
material (eg. Gold palladium, platinum palladium, or just platinum)?
2) Are there other materials that will work besides these metals that
would
give us equal service for small grain and a stable coating?
3) Are there operating conditions we could try that would still give us
good
coating but reduce the grain size of the film material?
Thanks for your help,

Mike

======================================
Michael D. Standing
BYU Microscopy Lab
401 WIDB
Brigham Young University
Provo, UT 84602

e-mail: Michael_Standing-at-byu.edu
phone: (801) 378-4011
fax: (801) 378-3937
======================================





From daemon Tue Jan 16 15:56:08 2001



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Tue, 16 Jan 2001 13:45:33 -0800
Subject: Bakelite Rings

Contents Retrieved from Microscopy Listserver Archives
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Ah, once again invaluable information, Thank you John, Fred and Nestor
too!!
I tried all the suppliers I could think of, except Buehler of course!

--
Sarah A.W. Lundberg
Electron Microanalysis and Imaging Laboratory
Department of Geoscience, UNLV
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010

EPMA Lab (702) 895-2660
SEM Lab (702) 895-2462
Office (702) 895-1134
Fax (702) 895-4064
Dept. Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMIL.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635




From daemon Tue Jan 16 15:59:16 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 16 Jan 2001 17:00:34 -0500
Subject: polarizing microscopes

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
Here's an LM-related question:
A group here needs to look at samples stained with picrosirius red
using polarizing optics. I have a microscope equipped with DIC, and
realize that if we pull the Wollaston prisms, we might be able to see
something, but I don't have a rotatable stage which would make it
easier to align their samples relative to the polarized light. Do
any of you out there in the New York City area have an appropriate
microscope? Or any ideas?
TIA,
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jan 16 16:19:05 2001



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 16 Jan 2001 17:15:35 -0500
Subject: mounting supplies - bakelite ring forms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sarah:

We at South Bay Technology offer such a product. They are available as our
Part Number RFP100-10 and cost $5.25 for a package of 10. We have them in
stock and can ship them out the same day you order them. I hope this
helps.

Best regards-

David
Writing at 3:03:09 PM on 01/16/2001

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Sarah Lundberg
}
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Hello listers-
I am looking for a supplier of bakelite ring forms 1 inch in diameter
(outside). Would someone contact me with that information. I can't
find anything on the web.
I appreciate your help!
Many Thanks,
Sarah
--
Sarah A.W. Lundberg
Electron Microanalysis and Imaging Laboratory
Department of Geoscience, UNLV
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010

EPMA Lab (702) 895-2660
SEM Lab (702) 895-2462
Office (702) 895-1134
Fax (702) 895-4064
Dept. Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMIL.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635


{


From daemon Tue Jan 16 16:30:38 2001



From: Pier, Julie (LNA) :      Julie.Pier-at-america.luzenac.com
Date: Tue, 16 Jan 2001 15:32:35 -0700
Subject: Electron diffraction pattern simulation software

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know of free software to simulate electron diffraction patterns
(space group info not necessary)?


From daemon Tue Jan 16 21:04:00 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 17 Jan 2001 00:58:45 -0200 (UOL)
Subject: TEM: problems with Spurr embedding of seeds

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Hi all,
I am working with seeds of Ilex paraguariensis (the maté). The aim of my research is to describe the ultrastructure (TEM) of the endosperm and the imature embryo. The samples (small dissected pieces) were fixed in glutaraldehyde and formaldehyde solution in phosphate buffer, washed, transferred for osmium (2% in 0,8% K3FeCN6, 1:1), dehydrated in acetone (10, 30, 50, 70, 90, 90 ,100, 100, 30 min in each step) and included in 1:3, 1:1, 3:1 and two changes of pure Spurr resin (24 hour in each step with continuous agitation). However, the tissues are very poorly infiltrated (endosperm and embryo with holes and soft parts). The endosperm cells have a lot of proteins and lipids as storage substances. I think that I should use a special protocol for this plant tissues, but I want to maintain the osmium fixation (lipid preservation). I tried the LR White and Unicryl resins with worst results. Does anybody suggest some thing?
Thank's.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy
Dept. of Botany - UFRGS
Brazil
e-mail: rinaldop-at-uol.com.br


From daemon Wed Jan 17 00:45:26 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Wed, 17 Jan 2001 07:40:08 +0100
Subject: Re: Staining Spurr's and LR sections for LM

Contents Retrieved from Microscopy Listserver Archives
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Hi Rosalie,

you will find a lot of information in "Histochemistry" by R.W. Horobin,
Stuttgart, G. Fischer 1982.

A few years ago I did some staining of plant cuticles (lets assume cutin is
similar to suberin for your purpose) with Sudan Black B in Epon sections.
SBB needs to be dissolved in hot alcohol but it worked quiet well on my
Epon semis. I even managed to dissolve the resin in KOH before staining and
got good results ... A recipe for SBB staining you will find in Bronner,
R., 1975: Simultanous Staining of Lipid and Starch in Plant Tissues. Stain
Technology 50(1):1-4.

I do not know if you want to use Toluidin Blue O, but TBO is a water based
stain for acid polyanions like pectins, DNA/RNA and stains cytoplasm too.
It will stain paraffin and resin sections and is easy and relatively safe
to use. A very basic paper describing it is: Sakai, S.W, 1973: Simple
method for differential staining of paraffin embedded plant material using
Toluidin Blue O. Stain Tech 48(5):247-249.

Hope that helps you,

Joachim

Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350



From daemon Wed Jan 17 02:04:04 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Wed, 17 Jan 2001 10:01:26 +0100
Subject: Antwort: TEM: problems with Spurr embedding of seeds

Contents Retrieved from Microscopy Listserver Archives
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------- Forwarded message follows -------
} From: Alexander Tikhonovski {tikhonov-at-mpi-halle.mpg.de}
To: "Pier, Julie (LNA)" {Julie.Pier-at-america.luzenac.com}



Hi Rinaldo,

I have been working on the ultrastructure of orchid stigmas for a few
years, this tissue contains a large amount of lipids and intercellular
cutin, debris from plasmolysed "nutritive cells", slimy pectins etc ...
very difficult to embed - at least that was what everbody told me when I
started.
As in the orchid column the pollen (pollinia in this case) is still there,
in most cases I embedded even the pollen with its sporopollenin, a lipidic
substance usually extremely bad to embed (sections may break out at the
interface to the resin).

In the beginning I tried Spurr and had very poor results. Then I switched
to Epon although everybody told me that this would work even worse because
of the high viscosity of Epon (= bad infiltration).

But I could achieve godd embedding with Epon for my tissue. Here is how I
did it:

Specimen: Orchid buds, columns and stigmas, preferably smaller then 2x2x2
mm
Fixation: 3% GAD in cacodylate buffer pH7.2 for at least 24 h
Wash a few times in buffer and then in a.d.
Stepwise dehydration in acetone: 30, 50, 70, 90, 100, 100% for 20 min each
Intermedium acetontril 3 x 100% for 10 min each
Embedding in Epon (Agar 100 Resin Kit): No movement of the specimens
needed, just use Eppendorf tubes or something similar
acetonitril:Epon 3:1 overnight
acetonitril:Epon 1:1 4 h
acetonitril:Epon 1:3 4 h
pure Epon overnight
change to Epon + accelerator
Polymerisation in flat embedding form 48 h at 60°C

I used the acetonitril as a substitute for propylene oxide simply because
it is said to be less poisonous. Maybe its the acetonitril step that is
responsible for the good infiltration - I really do not know, but it worked
well, so "never change a winning team".

Hope that helps you,

Joachim

Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350




From daemon Wed Jan 17 03:49:44 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Wed, 17 Jan 2001 11:44:52 +0200
Subject: ICEM-15

Contents Retrieved from Microscopy Listserver Archives
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{bold} *** ICEM-15 *** {/bold}


"World Focus on Microscopy"


15th International Congress on Electron Microscopy

Durban, South Africa


1 - 6 September 2002


www.icem15.com


{center} A conference and trade exhibition on the technology, techniques
and applications of all forms of microscopy to be held under the
auspices of the International Federation of Societies for Electron
Microscopy and the Microscopy Society of Southern Africa. {/center}


The Organisers of ICEM-15 are pleased to extend an invitation to

anyone involved in microscopy in any of its various forms to use

this opportunity to hear from world authorities about the latest

developments in your areas of interest, meet with colleagues,

make new friends, present results, see and test the latest

technology, and last but not least, enjoy the best of South Africa's

warmth and hospitality.


Look at the web site (www.icem15.com) for more information about
this exciting event.


If you wish to be included on the mailing list please click your reply

button and complete your contact details in the space below.

Alternatively you may register your information online when you
visit the congress web site (www.icem15.com).


Best regards


Robin Cross

Chairman : ICEM-15



Please include me on the mailing list for ICEM-15.


Name:

Postal address:

Email address:

Tel:

Fax: {color} {param} 0100,0100,0100 {/param}



From daemon Wed Jan 17 05:37:16 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 17 Jan 2001 06:31:33 -0500
Subject: Re: Metal for sputter coating targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
The obvious grains of Au are the reason that the Au/Pd alloy is used.
It should be less apparent with a Au/Pd target.
Ken Converse
owner
Quality Images
Delta, PA

Michael D. Standing wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers:
}
} In our lab we have been sputter coating with gold since the dawn of time.
} This has essentially been the "traditional" metal we have used for coating
} our non-conductive samples. We have found that in some of our applications
} that the gold grain is to large and is causing us problems in visualizing
} some of the smaller structures (primarily in engineering samples) that we
} are interested in. We currently sputter our samples for 1 to 4 minutes with
} a current reading of 18 to 20 mA using an argon atmosphere in the coater.
} We are aware that chromium and/or osmium coating are preferential to gold
} sputtering, but those technologies are not available to us at this time.
} What I would like to know is:
} 1) What are the advantages of using something other than gold as the target
} material (eg. Gold palladium, platinum palladium, or just platinum)?
} 2) Are there other materials that will work besides these metals that would
} give us equal service for small grain and a stable coating?
} 3) Are there operating conditions we could try that would still give us good
} coating but reduce the grain size of the film material?
} Thanks for your help,
}
} Mike
}
} ======================================
} Michael D. Standing
} BYU Microscopy Lab
} 401 WIDB
} Brigham Young University
} Provo, UT 84602
}
} e-mail: Michael_Standing-at-byu.edu
} phone: (801) 378-4011
} fax: (801) 378-3937
} ======================================
}
}
}
}
}



From daemon Wed Jan 17 08:02:27 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 17 Jan 2001 08:58:04 -0500
Subject: Re: Metal for sputter coating targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,

I would suggest you try gold/palladium. It has a smaller grain size and
the same reflectivity.

John Arnott

Disclaimer: Ladd Research sell targets for most types of sputter
coaters.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


Michael D. Standing wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Dear Listers:
}
} In our lab we have been sputter coating with gold since the dawn of time.
} This has essentially been the "traditional" metal we have used for coating
} our non-conductive samples. We have found that in some of our applications
} that the gold grain is to large and is causing us problems in visualizing
} some of the smaller structures (primarily in engineering samples) that we
} are interested in. We currently sputter our samples for 1 to 4 minutes with
} a current reading of 18 to 20 mA using an argon atmosphere in the coater.
} We are aware that chromium and/or osmium coating are preferential to gold
} sputtering, but those technologies are not available to us at this time.
} What I would like to know is:
} 1) What are the advantages of using something other than gold as the target
} material (eg. Gold palladium, platinum palladium, or just platinum)?
} 2) Are there other materials that will work besides these metals that would
} give us equal service for small grain and a stable coating?
} 3) Are there operating conditions we could try that would still give us good
} coating but reduce the grain size of the film material?
} Thanks for your help,
}
} Mike
}
} ======================================
} Michael D. Standing
} BYU Microscopy Lab
} 401 WIDB
} Brigham Young University
} Provo, UT 84602
}
} e-mail: Michael_Standing-at-byu.edu
} phone: (801) 378-4011
} fax: (801) 378-3937
} ======================================


From daemon Wed Jan 17 09:36:45 2001



From: Leslie Cummins :      gunther-at-aecom.yu.edu
Date: Wed, 17 Jan 2001 10:26:23 -0500
Subject: CryoEM sectioning

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers

I have been doing cryoultramicrotomy for the last year or so with varying
levels of success. I finally got some pretty pictures, so I know I can get
it right. Now I want to make it consistent.

So, my biggest problem appears to be with freezing. I am fixing my cells
with 4% para, 0.1% glut, 0.25M Hepes, rinsing with Hepes, embedding in 10%
gelatin and then infiltrating with 2.3M sucrose in a stepwise manner. I am
cutting the sample into *very* small blocks (less than 0.5mm) hoping to
infiltrate better. I then place the sample onto pins and drop into liquid
nitrogen to freeze. However, it always seems that only the very outer
layer of tissue/cells are well frozen. If I take a 1 micron section to see
how the tissue looks, I can't take another b/c it would bring me into a
freeze damaged area. I have tried using liquid proprane and liquid freon,
but the samples cracked when I attempted to section them.

I have now been given a very precious human sample that they want to
immunolabel on cryo thin sections, and I don't have enough tissue to make
lots of blocks to section just the outside.

If anyone has any suggestions to help me get good freezing deeper in the
tissue, I'd appreciate it.
TIA
Leslie


These are my pretty pictures:
http://www.aecom.yu.edu/aif/gallery/TEM/shields_immuno/tem_cryo.htm
Analytical Imaging Facility visit our web site: www.aecom.yu.edu/aif
Albert Einstein C. of M
1300 Morris Park Ave
Forchheimer 639
Bronx, NY 10461
718-430-3547


From daemon Wed Jan 17 10:15:40 2001



From: Cindy Kleier :      j-kleier-at-northwestern.edu
Date: Wed, 17 Jan 2001 10:10:19 -0800
Subject: Negatives-Need help find clear plastic sleeves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The sleeves need to be 3 1/4 x 4 in. I don't know where they were bought
previously and need help finding out where.

Thanks

Cindy K.

Joyce L. Kleier
Northwestern University, Evanston, IL. USA
j-kleier-at-northwestern.edu



From daemon Wed Jan 17 11:02:48 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 17 Jan 2001 11:55:27 -0500
Subject: Microtome Safety

Contents Retrieved from Microscopy Listserver Archives
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To all:

It always amazes me how much discussion can be generated on some topics!

I very rarely offer my 'two cents', but love reading through the comments.
On the issue of microtome safety: I have been an electron microscopist for
over 25 years (I started as a child prodigy!) and other than one incident early
on when I (stupidly) tried trimming a block by bringing the razor blade toward
me, I have NEVER had a problem with severed fingers, major lacerations, etc.

The aforementioned incident did result in stitches and I bear the scar proudly.

I do my trimming on a special holder on the Reichert Ultracut looking
through the binoculars and "map" out the block face by marking the
edges with the blade. Then I proceed very carefully (away from me
and the block) to trim the epon. I then do the rough sectioning on
the microtome itself. At times, we have used a jeweler's saw and
vise to trim away excess epon and get down to the tissue, etc.

I agree that gloves or other enhancements only add to the problem.

I believe the main thing to consider is: "Think before you cut!"

Peggy Sherwood
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed Jan 17 11:22:56 2001



From: Joseph Passero :      jp-at-spacelab.net
Date: Wed, 17 Jan 2001 12:20:01 -0500
Subject: [LM - Stereo] Wanted AO Stereo Microscope Part

Contents Retrieved from Microscopy Listserver Archives
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Would any one know who or have a AO coaxial illuminator (available for sale) for a AO model 580
stereo zoom pod?

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net

Shop online without a credit card
http://www.rocketcash.com
RocketCash, a NetZero subsidiary


From daemon Wed Jan 17 13:49:20 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 17 Jan 2001 14:45:44 -0500
Subject: Re: Negatives-Need help find clear plastic sleeves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cindy Kleier wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} The sleeves need to be 3 1/4 x 4 in. I don't know where they were bought
} previously and need help finding out where.
}
} Thanks
}
} Cindy K.
}
} Joyce L. Kleier
} Northwestern University, Evanston, IL. USA
} j-kleier-at-northwestern.edu

Polysciences in Warrington, PA 800-523-2575
Electron Microscopy Sciences in Ft. Washington, PA 800-523-5874


Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Jan 17 14:14:25 2001



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Wed, 17 Jan 2001 13:42:33 -0600
Subject: SEM, used model available

Contents Retrieved from Microscopy Listserver Archives
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Hennepin County Medical Center, Minneapolis, MN has a used ISI-60A SEM
available to anyone interested. Supporting equipment also available
include: Eiko Multicoater, model VX-10A and an OMAR critical point dryer,
model SPC 900/EX.



From daemon Wed Jan 17 14:31:37 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Wed, 17 Jan 2001 15:28:02 -0500
Subject: TEM specimen prep short course (there is no snow in Florida in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TEM Specimen Preparation Short Course!!!!

With emphasis on Tripod Polishing and Focused Ion Beam Specimen Preparation

.will be offered in conjunction with Surface Analysis 2001 and the Joint
Annual Meetings of the
Florida Society for Microscopy and the Florida American Vacuum Society in
Orlando at the University of Central Florida March 14-16, 2001.

Instructors:

Ron Anderson, IBM
Fred Stevie, Lucent Technologies
Lucille Giannuzzi, UCF

Vendor Sponsors will be participating!

To register, please go to www.dce.ucf.edu

For technical information please contact Lucille Giannuzzi at lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Wed Jan 17 14:41:12 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Wed, 17 Jan 2001 15:23:23 -0600
Subject: Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,
One of my readers has asked the following question. If you can help, kindly
contact her direct.
Don Grimes, Microscopy Today

Do you know or can you ask your readers about the possibility of
Electrostatic Discharge damage caused by an SEM? Is this a problem? My
company is reluctant to have me examine flight-ready devices in the SEM
because of this possibility. I can't find any information on the
subject. Thanks for any help you can give me.

Virginia Harper
vbharper-at-west.raytheon.com



From daemon Wed Jan 17 15:38:38 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 17 Jan 2001 15:31:22 -0600
Subject: Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, one of the (supposedly true)legends in our facility is that a student
was knocked out of a chair one day long ago by a major spark from an old
JSM35 SEM. But maybe that's not what you meant.... ;-)

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO  65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu {mailto:tindallr-at-missouri.edu}  
          http://biotech.missouri.edu/emc



-----Original Message-----
} From: Don Grimes [mailto:microtoday-at-mindspring.com]
Sent: Wednesday, January 17, 2001 3:23 PM
To: microscopy-at-sparc5.microscopy.com


Group,
One of my readers has asked the following question. If you can help, kindly
contact her direct.
Don Grimes, Microscopy Today

Do you know or can you ask your readers about the possibility of
Electrostatic Discharge damage caused by an SEM? Is this a problem? My
company is reluctant to have me examine flight-ready devices in the SEM
because of this possibility. I can't find any information on the
subject. Thanks for any help you can give me.

Virginia Harper
vbharper-at-west.raytheon.com



From daemon Wed Jan 17 15:47:32 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 17 Jan 2001 15:41:22 -0600
Subject: Teflon stirring paddle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Does anyone know where to find a Teflon stirrer/mixer attachment in the
shape of a corkscrew. We used to use one at the New Mexico State EM lab
(yup, that's you, Soumitra) for mixing resins, attached to a variable speed
drill-type mixer on a stand. I have never been able to locate another and
my stirring arm is about worn out.

Thanks

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Jan 17 18:00:52 2001



From: Boron nitride :      boronnitride-at-hotmail.com
Date: Wed, 17 Jan 2001 16:55:08 -0700
Subject: Software for generating atom arrangment in one specific atomic palne

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleage,
Does anybody know if there is a software that can draw the atom arrangments
in a specific crystalline plane? I know several software, such as
Crystalkit, Mactempas and Crystal Maker, can draw the atomic
projection, in which many layers are overlapped, however they can not
display the
atomic arrangments in one specific plane.

I would greatly appreciate if you could provide such kind of
information!

Sincerely,


James



_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com



From daemon Wed Jan 17 19:14:12 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 17 Jan 01 17:19:47 -0800
Subject: RE: Microtome Safety

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 17 Jan 01 17:19:47 -0800
Subject: RE: Microtome Safety

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Reply to: RE: Microtome Safety
I too have been surprized by the response to this subject.
I wonder if everyone who uses Lowicryl resin also hand trims the blocks with a blade?


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


}
} To all:
}
} It always amazes me how much discussion can be generated on some topics!
}
} I very rarely offer my 'two cents', but love reading through the comments.
} On the issue of microtome safety: I have been an electron microscopist for
} over 25 years (I started as a child prodigy!) and other than one incident early
} on when I (stupidly) tried trimming a block by bringing the razor blade toward
} me, I have NEVER had a problem with severed fingers, major lacerations, etc.
}
} The aforementioned incident did result in stitches and I bear the scar proudly.
}
} I do my trimming on a special holder on the Reichert Ultracut looking } through the binoculars and "map" out the block face by marking the } edges with the blade. Then I proceed very carefully (away from me } and the block) to trim the epon. I then do the rough sectioning on } the microtome itself. At times, we have used a jeweler's saw and } vise to trim away excess epon and get down to the tissue, etc.
}
} I agree that gloves or other enhancements only add to the problem.
}
} I believe the main thing to consider is: "Think before you cut!"
}
} Peggy Sherwood
} -- } Peggy Sherwood
} Lab Associate, Photopathology
} Wellman Laboratories of Photomedicine (W224)
} Massachusetts General Hospital
} 50 Blossom Street
} Boston, MA 02114
} 617-724-4839 (voice mail)
} 617-726-6983 (lab)
} 617-726-3192 (fax)
} sherwood-at-helix.mgh.harvard.edu
}
}
}
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From daemon Wed Jan 17 19:54:18 2001



From: Gordon Nord :      gnord-at-mindspring.com
Date: Wed, 17 Jan 2001 20:52:16 -0500
Subject: Re: Software for generating atom arrangment in one specific atomic palne

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James,

Perhaps you are familiar with an older version but CrystalMaker can limit the
size of your structure to one atomic plane and draw an opaque or translucent
plane in any color parallel or coincident ot the plane of interest. Check it
out.

{http://www.crystalmaker.co.uk}



Boron nitride wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Colleage,
} Does anybody know if there is a software that can draw the atom arrangments
} in a specific crystalline plane? I know several software, such as
} Crystalkit, Mactempas and Crystal Maker, can draw the atomic
} projection, in which many layers are overlapped, however they can not
} display the
} atomic arrangments in one specific plane.
}
} I would greatly appreciate if you could provide such kind of
} information!
}
} Sincerely,
}
} James
}
} _________________________________________________________________
} Get your FREE download of MSN Explorer at http://explorer.msn.com

--
Gordon Nord
Scientist Emeritus
US Geological Survey
Email gnord-at-mindspring.com




From daemon Thu Jan 18 04:09:39 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Thu, 18 Jan 2001 10:24:30 +0100
Subject: Antwort: CryoEM sectioning

Contents Retrieved from Microscopy Listserver Archives
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Hi Leslie,

I think you are doing everything right (fixation, rinsing, 10% gelatin,
0.5mm size, drop into LN2), but maybe it is the STEPWISE infiltration with
2.3 M sucrose that does not work well for your cells. How long do you do
the infiltration? At what temperature?

You probably get a gradient of sucrose concentration from the edges to the
center of your blocks because the lower suc concentrations enter the block
rapidly while the higher concentrations need more time?! The different
concentrations probably do not have time enough at low temperatures (i.e.
+4°C) to mix/substitute properly.

I would recommend to do the infiltration in an Eppendorf vial (app. 1ml)
DIRECTLY in 2.3M sucrose at +4°C (your gelatin is not fixed I assume, so
you need the low temperature for the gelatin to stay solid). Infiltrate for
AT LEAST 2 HOURS on a rotating table.

Jan Slots group in Utrecht, The Netherlands achieves very good results with
that method. They are giving a highly recommendable CRYOCOURSE from 3rd -
12th July 2001 in cooperation with Leica (I am doing a tiny little bit of
it). If you are interested I can send you detailed information.

Hope that helps you,

Joachim



Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350



From daemon Thu Jan 18 04:09:56 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 18 Jan 2001 04:10:09 -0600
Subject: RE: Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your question is quite frankly hard to fathom. In an SEM, the sample is
subjected to up to 50 KV electron bombardment intentionally at up to
several hundred nanoamps in an area as small as a few tenths of a square
millimeter. Exactly what measure are you considering as electrostatic
discharge? Voltage, current or radiation flux density? And what specs are
you holding your electronics to? If lightning strikes or EMP effects are
considered, the SEM's effects are probably minimal.

50 KV can certainly be considered an electrostatic potential. 50 KV at 100
nanoamps sample current translates to only 5 milliwatts. However, that
amount of power translated to the sub-millimeter scales of an electron
microscope can add up, as can the effects mentioned when considering such
small structures.

A rough estimate shows that a 30 KV beam at 100 nA sample current and an
excitation surface on the sample of 100 micrometers diameter yields a huge
energy density at the surface. Assuming a subsurface absorption sphere of
around 200 micrometers diameter, obviously the radiation flux densities are
quite high. I leave it to you to calculate the actual figures based on the
materials you are sampling.

However, given your internet domain and the specific question asked, I have
to wonder what reservations your company has. If there are electrostatic
weaknesses in the circuitry you want to examine, is it better to have these
brought out in R&D SEM examination or in field exposure to lightning, EMP
and ECM? If I were in the left seat, I would want some assurance that
those envelope edges had been explored. My intuitive side would tend to
believe that the potential lightning, EMP and ECM effects would likely be
as concentrated, if not more so, that the SEM beam energies in the
nanoscale structures of the circuitry you are probably using. In fact, the
electron beam energies may be the best method you have of simulating the
localized energy density effects of these sources.


} Group,
} One of my readers has asked the following question. If you can help,
kindly
} contact her direct.
} Don Grimes, Microscopy Today
}
} Do you know or can you ask your readers about the possibility of
} Electrostatic Discharge damage caused by an SEM? Is this a problem? My
} company is reluctant to have me examine flight-ready devices in the SEM
} because of this possibility. I can't find any information on the
} subject. Thanks for any help you can give me.
}
} Virginia Harper
} vbharper-at-west.raytheon.com
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Jan 18 07:22:28 2001



From: jshields-at-cb.uga.edu
Date: Thu, 18 Jan 2001 08:21:23 -0500
Subject: Re: Negatives-Need help find clear plastic sleeves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We get them from Better Containers Mfg. Co.
530 Hyde Park
Hillside, Ill 60162
708-547-7272

There is also other companies (e.g. Consolidate Plastics Co., Inc.
330-425-3900) out there.

Good Luck

On 17 Jan 2001, at 10:10, Cindy Kleier wrote:

} The sleeves need to be 3 1/4 x 4 in. I don't know where they were
} bought previously and need help finding out where.
}
} Thanks
}
} Cindy K.
}
} Joyce L. Kleier
} Northwestern University, Evanston, IL. USA
} j-kleier-at-northwestern.edu
}


John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu


From daemon Thu Jan 18 07:39:56 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 18 Jan 2001 08:32:25 -0800
Subject: Re: Negatives-Need help find clear plastic sleeves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} The sleeves need to be 3 1/4 x 4 in. I don't know where they were bought
} previously and need help finding out where.

We carry Plastine sleeves for 3 1/4 x 4 negatives.
These sleeves are archival quality polyethylene and come in
a box of 1000.
We also carry Kodak Sleeves which come in boxes of 100,
and the Print File EM-6 page which will hold 6 negatives in a
page that fits a 3 ring binder.

George


George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com


From daemon Thu Jan 18 07:41:35 2001



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Thu, 18 Jan 2001 05:46:14 -0800
Subject: Re: Software for generating atom arrangment in one specific atomic palne

Contents Retrieved from Microscopy Listserver Archives
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"Boron",

I think EMS will also do what you want. There is a web site where you
can
preview some of the possibilities of this package.

http://cimesg1.epfl.ch/CIOL/ems.html

cheers,
John

John Heckman
MSM Department
Michigan State University

No affiliation with CIME; just an end user of EMS.





From daemon Thu Jan 18 08:15:26 2001



From: Majid :      majid.ghoddusi-at-anu.edu.au
Date: Thu, 18 Jan 2001 08:10:33 -0600
Subject: Freeze-Fracture replica cleaning

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Hello all

We are preparing Pt-C and C replicas from different biological material. For
cleaning the obtained replica we either use bleach (5.2%) or chromic acid
(10, 20 followed by 40%) with washes in between. The protocol usually works
well for most stuff but when it comes to oocytes from Xenopus we are usually
left with a lot of organic material still attached to the replica. If we
leave the replica in cleaning agent for a long time we end up with a very
brittle replica which can easily break.

Oocytes have got a reputation for being very sticky and difficult to clean.
We are considering to use plasma cleaning instead but I don't know what to
expect from that. I would appreciate it if you could share your 'tips and
tricks' with us.

Majid Ghoddusi



................................................................
Majid Ghoddusi, PhD
Protein Dynamics Unit
Department of Chemistry
Australian National University
ACTON 0200
Canberra
Tel: (02) 6125 4190
Fax: (02) 6125 0760
e.mail: majid.ghoddusi-at-anu.edu.au
web site: http://langevin.anu.edu.au/
...........................................................................
..........




From daemon Thu Jan 18 08:25:58 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 18 Jan 2001 09:25:44 -0500
Subject: Re: CryoEM sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Leslie,
One thing I can think of, is that you shouldn't drop your samples
into the liq. Nit. LN2 is a poor cryogen in that it boils when warm
things are placed into it, creating a pocket of nitrogen gas around
the sample, that acts as an insulating layer. Most of us do use
nitrogen to freeze our samples. I always hold the pin end of the
stub in a hemostat-type clamp and wiggle it as it freezes to try to
ensure an exchange of the cryogen and avoid too much of a gas bubble
around the sample. I'm sure you will get many other suggestions,
since there are so many of us out there doing this with varying
degrees of success.
Good luck,
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Jan 18 08:25:58 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 18 Jan 2001 11:21:25 -0500
Subject: Metal for sputter coating targets

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

ESD damage on semiconductor samples is quite real & has been documented for
years.
When CMOS circuits first came out this was a real concern.
Motorola & a number of other semiconductor companies has reams of papers on
this subject.

Contact me offline & I can see if I can dig up the info.

Regards,

Earl

----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Don Grimes'" {microtoday-at-mindspring.com} ;
{microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 18, 2001 2:10 AM


Hi

Just a few points which may help in your quest for better sputter coatings.

Sputter coaters fall into a number of categories based upon age. Each
category requires a slightly different set up procedure.

Class 1 - Coaters with a variac voltage control of up to 1500volts
Class 2 - Coaters with a variac voltage control of up to 2500volts
Class 3 - Cool coaters with a fixed voltage but a variable "deposition"
control

General - All coaters need approximately a 5cm target to specimen
distance. Coat a piece of paper the 2-3 minutes the paper should cover
the specimen bed, you will then see the distribution pattern of low and
high depostion areas. Make sure you put your specimen in a high deposition
area. Coating many specimens in one run may lead to uneven coatings as
each specimen has an affect on those near by, outgassing, partial shadowing
etc.

Class 1 need an operating voltage which you would guess is about 700 volts
on the voltage dial, Class 2 around 800 volts (i.e. as low as possible
whilst still striking a plasma). These coaters probably need a 1 minute
coating at 20mA for gold.

Class 3 coaters need a deposition rate set at 10mA and a 30 second coating
for gold.

With any coater increase the coating time and you increase thickness and
the possibility of visualizing the structure.

Multi coats increase the visibility of the metal structure.

Platinum deposits at half the rate but at less than half the grain size;
increase coating times but do not exceed the 20mA and 10mA settings as
described above.

Hope this helps

Steve Chapman
Senior Consultant Protrain
For consultancy and training by professionals World Wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com


From daemon Thu Jan 18 10:30:41 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 18 Jan 2001 11:29:22 -0500
Subject: Re: polarizing microscopes

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,

Thanks to all of you who have responded to my query about polarizing
microscopes. Among the responses was the name of someone nearby who
has a fully equipped instrument, and if all the "poor man's"
adaptations I've received don't pan out, I'll give that fellow a
call. Below are highlights of the responses I received. We can put
this topic to rest now.

Lee

***********************
Hello re: rotating stage.

Put some vaseline on top of your stage after removing the mechanical
stage, and invert an AOL CD on it. Makes an elegant rotating stage
for a scope.
************************
Alternatively, you can make a mini "Theta" stage by mounting any sort
of small turntable on your existing stage. Take off all the normal
slide holding/mechanical stage equipment and mount the turntable in
place. Parameters:
a. You need to open a hole in the center so that you can get light
through the slide.
b. You need some mechanism (as simple as a rubber band or an old
stage clip) which will allow you to slide the slide for XY; the
special stage will supply the rotation
c. Depending on the thickness of the turntable, you may need a long
working distance condenser; simple Abbe condensers are good, but for
true pol work, you need high NA

There are limits to this approach, especially in terms of centration,
but if they are just doing qualitative Pol, it's a good way to go.
***********************
Yes, as you wrote, if you remove the wallaston's from the
light path, you will indeed have a set up with crossed polarizers.
This is a good start, but as you suggested that still leaves a few
problems. You mentioned the rotating stage. As DIC is fairly popular,
you might well find someone around with a DIC stand that has a
rotatable stage. Really, for DIC work, the stage ought to rotate too,
because contrast in the specimen does change as a function of the
orientation of the sample. But there are two other very useful things
that you should look for in the stand.

1) Be sure that you can rotate either the polarizer or the analyser.
For DIC, it doesn't matter all that much if the analyser and
polarizer are not exactly crossed. But for polarized light they need
to be crossed as exactly as possible. You can look through the
eyepieces at a blank slide and rotate either the analyser or the
polarizer until the field gets maximally dark. Then stop.

2) A compensator of some kind is handy. That is because often the
sample has weak retardation and the compensator enhances that. If you
are lucky enough to find a real polarized light scope, it will have
several of these available. But if you use a DIC scope, then what you
can do is to experiment with various kinds of plastic that you find
lying around. Petri dish lids are a good bet, or clear tape or
plastic wrap stretched on a slide. Put this in the beam, somewhere
after the polarizer in a way that you can rotate to some extent.
You'll know you have got the right thing when you find something that
makes the nice dark field (from step 1) get bright as your rotate it.
***********************
I have made a 'poor-man's' polarizing microscope by putting one sheet of
polaroid material (glass or plastic) over the light source of an ordinary LM
and another sheet between the slide and the objective lens. I rotate the
sheet over the light source until I get the desired effect. Works great as
long as you do not need a high magnification lens that works close to the
specimen.

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Jan 18 10:48:35 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 18 Jan 2001 11:45:14 -0500
Subject: Facility management topics

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Okey, one last time. Please, if you haven't weighed in on your preference for topis (see below) by tomorrow, I will pick them based on response to date. There is a wide range of responses and I would love to have more to see if a few topics end up standing above the rest as to interest level. Also, don't forget to recommend presenters for specific topics.
And thanks to all who have already responded. Please don't stuff the ballot box by voting again.
Thanks,
Debby

=================


Fellow Microscopists:
As many of you know, the Facility Management session held at M&M2000 was very well received and there was a unanimous request for it to be continued. Therefore a similar session will be held at M&M 2001. I must finalize the topics within the next week or two. Since the purpose of this session is to discuss topics of interest to facility managers, I would like your input before the session is finalized.

The topics covered in the M&M 2000 session with transcripts published in Microscopy Today were:
1) Multi-user Facilities...managing users
2) Justification of Costs, cost recovery, electronic bookkeeping and billing
3) Equipment Maintenance: Vender service contracts vs. Independent providers vs. Insurance company/self-insure options.

The following are some suggestions that were gleaned from the evaluation forms and discussion during the session.

1) Training Users…courses, workshops, …what works for whom?
2) Scientific Ethics…What are our responsibilities as lab Managers?
3) Mission statements/lab organization/lab business plans/future planning issues
4) Staffing issues including training, part-time student help, Training course T.A’s
5) Daily issues…prioritizing use, prioritizing service projects, dealing with difficult users/customers
6) Legal issues involving data ownership, confidentiality of data, handling of potential evidence.
7) Justification for new equipment
8) Commercial use of university facilities
9) Microscopy as a research tool…integration of industry or campus facilities in coordination with other core facilities.
10) Issues unique to Multi-user and/or Service facilities and how such facilities can coexist.


The first 2 received the greatest number of requests. However there may be more relevant topics now. Please rank the 3 of highest priority to you or suggest additional topics of interest. I will sift through all replies at the end of next week and narrow them down to 3. Also please feel free to supply the names of individuals to lead specific discussion topics.
The format will be the same as the last session. A presenter will give a 10-15 min. introduction of the topic followed by ~30 min. open discussion.

Thanks,
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



From daemon Thu Jan 18 11:37:47 2001



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Thu, 18 Jan 2001 17:31:50 -0000
Subject: Sputtering targets

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Hi Mike,
Just a thought. When we tried to change to other metals we found that our
sputterer would handle Au, Au/Pd but not Pt. Might be worth checking yours
before spending serious money.
ttfn, Chris


From daemon Thu Jan 18 11:52:27 2001



From: Rehorek, Susan S. :      susan.rehorek-at-sru.edu
Date: Thu, 18 Jan 2001 12:50:21 -0800
Subject: Looking for TEM

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I am searching for a new TEM (hoping to write a grant to get funding for
one anyway - since I am not independantly wealthy). The one we currently
have is a JOEL 100S (and a crabby one, too boot).

I would like to know what is available. I would also like to know some
approximate prices.

Please reply off-list!

Thank you.
Susan J Rehorek, Ph.D.
Department of Biology
Slippery Rock University of Pennsylvania
PA, 16057-1326

ph: 724 738 2485
fax: 724 738 4782
email: susan.rehorek-at-sru.edu


From daemon Thu Jan 18 12:55:43 2001



From: Karen Pawlowski :      Karen.Pawlowski-at-worldnet.att.net
Date: Thu, 18 Jan 2001 12:47:08 -0600
Subject: Basal bodies

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Hi all,

I was wondering if there are any stains/methods that would allow the
identification of basal bodies or centromeres in a non-dividing cell.
I would like to determine the basal body position in a cell without
resorting to serial TEM. My thinking is that I might be able to do
it in whole cells with the right marker. Any comments?

Karen Pawlowski


From daemon Thu Jan 18 12:55:43 2001



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Thu, 18 Jan 2001 13:47:43 -0500
Subject: Re: Freeze-Fracture replica cleaning

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Majid,
Try 50% sulfuric acid for 2 hours to overnight. I used this successfully
some years ago on replicas from Xenopus oocytes.
Frank


At 08:10 AM 1/18/01 -0600, Majid wrote:
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From daemon Thu Jan 18 14:33:39 2001



From: Sue Hendricks :      sjh2e-at-virginia.edu
Date: Thu, 18 Jan 2001 16:15:54 -0500
Subject: LM: tongue paraffin embedding help

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Yes, EDS is very real and chip manufacturers go to great length (end
expense) to avoid them. However, I think the mechanism is different:

EDS means, that something collects static electricity (by walking on a
carpet, for example), then touches something that is at a very different
potential. That's what happens if you touch a metal after walking on that
carpet. The enormous potential difference create strong electric field that
finally ionize the air and you get "zapped". The strong fields can destroy
the electronics.

In an SEM the sample is usually grounded and does not see strong fields.
True, the electrons are accelerated to 30 KV or more, but the sample does
not see that, as the anode usually sits at ground level with the Cathode
being at - 30 kV. What you need to be concerned about here is the kinetic
energy that is transferred to the sample and can heat it up considerable. On
the other hand, if the sample is not grounded, there will be fields
developing. There are also other effects, such as residual organics getting
"cracked" and forming a residue on the sample, but that's a different story.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Earl Weltmer [mailto:eweltmer-at-home.com]
Sent: Thursday, January 18, 2001 7:24 AM
To: ars-at-sem.com; 'Don Grimes'; microscopy-at-sparc5.microscopy.com


Hi All,

ESD damage on semiconductor samples is quite real & has been documented for
years.
When CMOS circuits first came out this was a real concern.
Motorola & a number of other semiconductor companies has reams of papers on
this subject.

Contact me offline & I can see if I can dig up the info.

Regards,

Earl

----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Don Grimes'" {microtoday-at-mindspring.com} ;
{microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 18, 2001 2:10 AM


Can anyone offer any tips for embedding rat tongue tissue in paraffin? I
am having difficulty with complete infiltration (?) of the tissue. The
tongue has a dense muscle core that becomes hard during the process causing
shredding during sectioning. Any help would be much appreciated.

On a second note: This tissue has been injected with tritiated thymidine.
Has anyone ever heard of loss of radioactive label during tissue post-fix or
embedding?

thanks so much,

Susan Hendricks
University of Virginia
Department of Psychology
PO Box 400400
Gilmer Hall
Charlottesville, VA 22904

804.982.4755
802.982.4785 [fax]
sjh2e-at-virginia.edu



From daemon Thu Jan 18 15:26:28 2001



From: Michael D. Standing :      Michael_Standing-at-byu.edu
Date: Thu, 18 Jan 2001 14:29:09 -0700
Subject: Metal for Sputter Coating Targets -- Thanks and a summary

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Dear Listers:

Thank you for the numerous responses to my query about which metals and
operating conditions to consider for improving the grain size of the
coatings we have been getting from our sputter coater. Below is a brief
summary of the recommendations we have received. I will be experimenting
with several of these items to see where we ultimately want to end up with
this. I suspect that we will develop several protocols based on the
requirements of the experiment. Again thank you.

1) Gold/Palladium definitely has a smaller grain and we should see a
definite improvement by using it.

2) Platinum will have the finest grain size but it takes longer to deposit,
potentially causing damage to sensitive samples.

3) Platinum/Palladium is sometimes used as an alternative to Chromium
coating

4) Reduce the coating thickness as much as possible

5) Coat using higher vacuum / lower currents / lower voltage to reduce grain
size

6) Pulse the deposition

7) Decrease sample surface temperature

8) Gold may be the best alternative for samples where resolution is not as
much a factor as is sample integrity as the other metals require more energy
/ longer exposure to get an adequate coating.
======================================
Michael D. Standing
BYU Microscopy Lab
401 WIDB
Brigham Young University
Provo, UT 84602

e-mail: Michael_Standing-at-byu.edu
phone: (801) 378-4011
fax: (801) 378-3937
======================================




From daemon Thu Jan 18 16:02:48 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 Jan 2001 13:55:00 -0800
Subject: Re: Sputtering targets

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Interesting. What was it about the unit that precluded Pt?
Did the maker supply Pt targets or did you just try your own?
It would seem that if Pt target are factory supplied, they should
work with the system. My Hummer does Au, Au/Pd and Pt
very well. No problems.

gary g.



At 09:31 AM 1/18/01, you wrote:
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From daemon Thu Jan 18 16:59:05 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 18 Jan 2001 15:08:50 -0800
Subject: Re: Freeze-Fracture replica cleaning

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Hello Majid

What I did many years ago, I treated the replica with enzymes to degradate
the most of the sample and then I did clean the replica in 20-40%
H2SO4. The enzymes you could use depends from nature of your contamination
on the replica. I did use proteinase-K. It's very active enzyme if you
need to have deal with proteins. I assume that, because your
containerizations are survived even in chromic-mixture (difficult to
impress to me) it may be some poly-sugars or something like that. So, you
may try the particular enzymes. There are plenty of them in the Sigma Catalog.

You may also use full-strength chromic-mixture. In theory it may not
affect the Pt-C replica. I don't like the bleach at all,
personally. Plasma cleaning will affect the integrity of the carbon layer,
I believe.

Good luck.

Sergey.

At 08:10 AM 1/18/01 -0600, you wrote:
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------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Thu Jan 18 17:05:47 2001



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Thu, 18 Jan 2001 17:58:27 -0500
Subject: Re: CryoEM sectioning

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Hi Leslie,

I've never had a problem with freeze damage and I've done this a lot...I
can't see anything obviously wrong in what you're doing..The few things I
can think of are:

- wash your cell pellet in PBS with 0.12% glycine before putting them in
the gelatin. This will quench free aldehyde groups from the fix and avoid
crosslinking the gelatin (I'm not sure this would affect the sucrose
infiltration..?? but..maybe..I never tried not quenching)

- avoid putting the cells in gelatin (if you can). If you have tissue
there's certainly no need for gelatin. Cut the fixed tissue in to small
cubes, wash in PBS/glycine for 15 minutes then infiltrate in 3 drops of 2.3
M sucrose at RT for a total of 15 minutes (30 minutes for tissue pieces),
put a piece of the pellet/tissue on the pin and put into LN2.

( I only do gelatin embedding if I have cells that have been fixed in
suspension for some reason - they will never stay together as a pellet in
the sucrose! If you spin the cells down in a microfuge tube in the fixative
and let them fix as a pellet, they will stay together really well without
the gelatin (you can get the pellet out of the microfuge tube with a
toothpick)

- If you need to do gelatin, try putting the gelatin embedded cells in 2.3M
Sucrose (not step-wise) and incubate over night in the fridge.

good luck!
don't hesitate to contact me if you have more problems.

Maria

At 10:26 AM 1/17/01 -0500, Leslie Cummins wrote:
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____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html


From daemon Thu Jan 18 17:44:13 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Thu, 18 Jan 2001 18:40:50 -0500
Subject: Re: Question

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Virginia,

The kinetic energy referred to by Michael can be a very real problem
for your sample in the SEM.

As far as the concern over Electrostatic Discharge damage goes, it may
not look the same as that caused by somebody's finger discharging
through the part, but it will be real. I have analyzed samples with both
types
of damage. In the SEM, floating nodes of circuitry may charge up from the
beam. When they exceed the breakdown voltage of the dielectric, such
as a CMOS gate oxide, a very large, very short in duration, current spike
will occur and cause extreme localized heating. This causes a hole in
the oxide, filled with alloyed silicon, causing a short circuit. Another
way
for this to happen, is for the interlevel dielectric to build up a charge
of
electrons, and then suddenly discharge through a part of the circuitry.
Both
of these mechanisms have given me problems while trying to localize
resistive via contacts with Resistive Contrast Imaging in the SEM, where
the specimen absorbed current is imaged. The defect "heals" when it is
blown through.

Another problem with looking at circuits that you hope to operate after
SEM observation, is the electron beam can "implant" electrons in the
chips structures and affect the proper operation of the circuitry. This is
especially true at the higher accelerating voltages.

I hope this helps.

Darrell Miles
Failure Analyst
IBM Microelectronics Analytical Services
Fishkill, New York



From daemon Thu Jan 18 17:44:13 2001



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Thu, 18 Jan 2001 18:38:39 -0500
Subject: RE: Question

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} Yes, EDS is very real and chip manufacturers go to great length (end
} expense) to avoid them. However, I think the mechanism is different:
}
} EDS means, that something collects static electricity (by walking on a
} carpet, for example), then touches something that is at a very different
} potential. That's what happens if you touch a metal after walking on that
} carpet. The enormous potential difference create strong electric field that
} finally ionize the air and you get "zapped". The strong fields can destroy
} the electronics.
}
} In an SEM the sample is usually grounded and does not see strong fields.
} True, the electrons are accelerated to 30 KV or more, but the sample does
} not see that, as the anode usually sits at ground level with the Cathode
} being at - 30 kV. What you need to be concerned about here is the kinetic
} energy that is transferred to the sample and can heat it up considerable. On
} the other hand, if the sample is not grounded, there will be fields
} developing. There are also other effects, such as residual organics getting
} "cracked" and forming a residue on the sample, but that's a different story.
}
} Michael
}
} Michael Bode, Ph.D.

Not really true as backscattered electrons as well as x-rays generated from
the sample can have an energy upto the acceleration voltage. The anode is
grounded to create the 30KeV field which accelerates the electrons. The
electrons shoot through the hole in the anode, through lenses (and other
things) all the way to the sample, at 30KeV. Check any source on how the
SEM works or for that matter how a basic electron accelerator works.

As for the EDS (static discharge that is) question, why would a chip maker
use SEM to analysis the effect of static discharge if the SEM could cause
the effect in the first place. Check out the older chip makers data books,
plenty of SEM photos of EDS effects. An SEM would be pretty useless for IC
failure analysis if it caused EDS damage.

EDS is destructive from the current density, not the field density. Too
much current through a sub-micron conductor blows up the conductor in the
same way electrical wires vaporize under too much current. EDS protection
is handled through shunting the current before it gets to the sensitive
areas. Most modern ICs are EDS protected, some (like IO drivers) more than
others. Old hat to chip makers and not much expense compared to the rest of
the design.

I would suggest this, try it. If you have EDS problems you will see the
results. If you see the results, send the design back to R&D for further
development. If you are nervious about 30KeV, use a lower voltage, that's
what it is there for.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Thu Jan 18 18:37:01 2001



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Thu, 18 Jan 2001 19:30:56 -0500
Subject: RE: Question

Contents Retrieved from Microscopy Listserver Archives
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One more comment,

The orignal question was

} } } Do you know or can you ask your readers about the possibility of
} } } Electrostatic Discharge damage caused by an SEM? Is this a problem? My
} } } company is reluctant to have me examine flight-ready devices in the SEM
} } } because of this possibility. I can't find any information on the
} } } subject. Thanks for any help you can give me.

Virginia , if you are suggesting to examine these devices and then install.
Very bad idea for any sort of testing besides operational on shipping
product. This is never done for production that goes out the door. That's
what product testing is all about. You take samples from production, then
subject them to things that they will see over their life. If they fail, a
statistical analysis will tell you their failure rate. Or if they cannot
fail (because of were they are used), tells you to try again.

Flight-ready devices have very specific rules and regulations, I can
understand their reluctance. Keeps things in the air, in the air. Why do
you need to examine by SEM? Do you need to examine every one or will a
statistical sample of production devices work as well. IC makers don't EDS
test every chip they ship yet they are EDS rated. They test samples from
pre-production and then test production samples every now and then. The
tested devices are not returned to production.

If these devices are expensive (most flight-ready devices are) and your
need for examination is important, then the company should have no problem
pulling some production devices for you to use. Once used, they do not ship
but are stored or destroyed. If you company says it's too expensive, then
you have a managment problem because product testing costs should have been
built into the selling price of the devices. It's part of NRE,
non-returnable expenses.

For a flight-ready device example, say you have a box of electronic that
must survive 100Gs. You don't subject every box to 100Gs and say ship the
ones that survived. You design for 100Gs. You test for 100Gs. You start
production and pull samples for testing at 100Gs. Over life of the
production and whenever the production process changes, you test for 100Gs.
Everytime you test, survive or fail, you open the box to check if there is
an unseen problem that will cause a future failure because you performed
the test. Product testing 101.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Thu Jan 18 22:02:32 2001



From: Harry Walsh :      h_walsh-at-acs.org
Date: Thu, 18 Jan 2001 21:56:26 -0600
Subject: ACS LM Short Course at PittCon

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"Applied Optical Microscopy" - an ACS Short Course, offered in conjunction
with PITTCON 2001 in New Orleans.

A three-day, hands-on course in light microscopy, including units on
contrast techniques, becoming a better consumer, digital imaging, and
quantitative polarized light.

March 2-4, 2001, Hyatt Regency Hotel, New Orleans, Louisiana
Tuition: $995 for non-ACS members, $895 for ACS members. (Note: Tuition does
not include housing or meals.)


For more information about the course or to register, contact the American
Chemical Society at shortcourses-at-acs.org or 800-227-5558, extension 4508.
Or, visit the website at www.acs.org/shortcourses.



**********************************************************
Harold G. Walsh
Department of Continuing Education
American Chemical Society
1155 Sixteenth Street, NW
Washington, DC 20036

Phone: 800-227-5558, ext. 4507, or 202-872-4507
Fax: 202-872-6336

Visit our Web site at: www.acs.org/shortcourses
**********************************************************




From daemon Thu Jan 18 22:40:44 2001



From: Al Coritz :      al-at-boeckeler.com
Date: Thu, 18 Jan 2001 21:57:52 -0700
Subject: Freeze-Fracture replica cleaning

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Quite Correct.

Usually 5% of the lot is pulled for testing.
The evaluation for the entire lot is based upon the test results of the
representative 5%.

Sometimes examining a part under the SEM will cause damage depending upon
the construction of the part.
The damage is artifact and has nothing to do with the part integrity.
I think it is this damage that Raytheon is most concerned.

The emphasis for SEM examination of semiconductor has shifted from 15KV to
20KV, to lower (up to 2KV)
accelerating voltages. Resolution at low KV suffers and hence the
untilization of field emission guns.


Regards All,


Earl

----- Original Message -----
} From: "Scott D. Davilla" {davilla-at-4pi.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 18, 2001 4:30 PM


Majid:

The common cleaning methods for FF replicas include: Conc. Bleach, 70%
Sulfuric acid, or 50%Chromic acid. These work for almost everything I have
encountered. For the others I recommend Fisher Scientific Glass Cleaning
Solution*. Start with 10% increasing to 50% overnight in a humidified
chamber with a cover.

If you don't take care to slowly increase the conc. of ANY of the cleaning
solutions you take the chance of "cooking" the remaining biological material
to the replica. This maybe what is happening. Several labs I work with are
using Xenopis oocytes and don't encounter problems with the replicas and
they use only Bleach. Plasma cleaning replicas may also "cook" the replica
too but I don't know for certain.

I think the brittleness you are encountering is related strictly to the
replication process and not the cleaning. I would need more information on
your protocol to comment on what might be causing it. Pt/C replicas are
stabile in all of the solutions mentioned above for extended cleaning times.

* I have no financial interest in Fisher Chemicals or Products.


Al Coritz
Sales & Service Manager
RMC-Boeckeler Instruments
4650 S. Butterfield Dr.
Tucson, AZ 85714
Voice: 520-745-0001
Cell: 520-465-3598
Fax: 520-745-0004
Email:Al-at-Boeckeler.com
Website:RMCProducts.com



-----Original Message-----
} From: Majid [mailto:majid.ghoddusi-at-anu.edu.au]
Sent: Thursday, January 18, 2001 7:11 AM
To: Microscopy-at-sparc5.microscopy.com




Hello all

We are preparing Pt-C and C replicas from different biological material. For
cleaning the obtained replica we either use bleach (5.2%) or chromic acid
(10, 20 followed by 40%) with washes in between. The protocol usually works
well for most stuff but when it comes to oocytes from Xenopus we are usually
left with a lot of organic material still attached to the replica. If we
leave the replica in cleaning agent for a long time we end up with a very
brittle replica which can easily break.

Oocytes have got a reputation for being very sticky and difficult to clean.
We are considering to use plasma cleaning instead but I don't know what to
expect from that. I would appreciate it if you could share your 'tips and
tricks' with us.

Majid Ghoddusi



...............................................................
Majid Ghoddusi, PhD
Protein Dynamics Unit
Department of Chemistry
Australian National University
ACTON 0200
Canberra
Tel: (02) 6125 4190
Fax: (02) 6125 0760
e.mail: majid.ghoddusi-at-anu.edu.au
web site: http://langevin.anu.edu.au/
..........................................................................
.........





From daemon Thu Jan 18 23:51:03 2001



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Thu, 18 Jan 2001 21:47:57 -0800 (PST)
Subject: Re: Basal bodies

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Hi Karen -
I've worked with ciliates (i.e., eukaryotic microorganisms -- one common
example is Paramecium), and there are several silver stains that, when
properly executed, allow the user to clearly visualize basal bodies under LM
brightfield. They are: Klein silver stain (using silver nitrate);
Chatton-Lwoff silver stain (using a salinated gel in combo with silver
nitrate); Rio Hortega ammoniacal silver stain (using a combo of ammonium
hydroxide and silver nitrate), and Protargol stain (using proteinaceous
silver nitrate). There are protocols for these stains in a protozoology book
by Kirby (I forget the title offhand). Any of these stains may help you.
Good luck with the basal body visualization!
Nelson Conti



On Thu, 18 Jan 2001 12:47:08 -0600, Karen.Pawlowski-at-worldnet.att.net wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hi all,
|
| I was wondering if there are any stains/methods that would allow the
| identification of basal bodies or centromeres in a non-dividing cell.
| I would like to determine the basal body position in a cell without
| resorting to serial TEM. My thinking is that I might be able to do
| it in whole cells with the right marker. Any comments?
|
| Karen Pawlowski
|


164 Ferne Court
Palo Alto, CA 94306
(650) 494-0472
[NelsonC51-at-excite.com]





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From daemon Fri Jan 19 05:02:01 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 19 Jan 2001 10:56:44 +0000
Subject: Re: CryoEM sectioning

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Leslie

one way that you could improve the cooling properties of your liquid
nitrogen is by 'slushing' it. This simply involves supercooling liquid
nitrogen by using latent heat of evaporation. A simple rotary pump and
vacuum chamber is used to evaporate gas off the liquid until a slush of
solid nitrogen is produced. If air is leaked into the chamber the solid
nitrogen melts and the process should be repeated 2 or 3 times to thoroughly
cool the nitrogen. The resulting liquid/slush should be handled with great
care because it does not boil when warm objects are plunged into it (i.e. it
will freeze specimens and you more quickly). It can be used for a few
minutes after production and can be tested simply by dropping small metal
objects in (if they 'fizz' it's no longer cool enough).

If you intend to try this you will need to experiment with quantities and
containers (about 100-200ml in a 200-400ml PTFE or metal beaker) and take
care that the nitrogen doesn't all spurt out when the solid forms on its
surface. You will need a vacuum chamber design where chilling and nitrogen
spillage will not cause damage, you can view the process through a window
and bleed air in quickly. I have used freeze drying units and a
home/lab-made chamber made out of an old film desiccator and they both
worked well. If you're careful and have some form of insulated drip tray you
might try an ordinary vacuum coater with just the roughing/rotary pump.

I'm sure there is plenty of published information on this technique (perhaps
in a Hayat book or other cryo technique texts) and I apologise if you were
already aware of it. If my memory serves me well, the last paper I looked at
stated that pentane etc. were better cryogens but that slush was a lot
better than liquid nitrogen.

Good luck

Malcolm Haswell
e.m. unit
University of Sunderland
UK


Leona Cohen-Gould wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
} Hi Leslie,
} One thing I can think of, is that you shouldn't drop your samples
} into the liq. Nit. LN2 is a poor cryogen in that it boils when warm
} things are placed into it, creating a pocket of nitrogen gas around
} the sample, that acts as an insulating layer. Most of us do use
} nitrogen to freeze our samples. I always hold the pin end of the
} stub in a hemostat-type clamp and wiggle it as it freezes to try to
} ensure an exchange of the cryogen and avoid too much of a gas bubble
} around the sample. I'm sure you will get many other suggestions,
} since there are so many of us out there doing this with varying
} degrees of success.
} Good luck,
} Lee
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175



From daemon Fri Jan 19 05:09:16 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 19 Jan 2001 05:06:19 -0600
Subject: Re: Question

Contents Retrieved from Microscopy Listserver Archives
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} }
} } The orignal question was
} }
} } } } } Do you know or can you ask your readers about the possibility of
} } } } } Electrostatic Discharge damage caused by an SEM? Is this a
problem?
} My
} } } } } company is reluctant to have me examine flight-ready devices in
the
} SEM
} } } } } because of this possibility. I can't find any information on the
} } } } } subject. Thanks for any help you can give me.
} }

Having worked on aircraft parts you will never get to test a flight ready
part and get it declared flight ready again. I expect an inspector will
destroy it in some way when you are finished with it. If you think about
it a little you wouldn't want it any other way. The device was not
designed to be run through a SEM so even if it was totaly safe it would be
out of the queston.

If you need to look at one look at one that has been used in other testing
or has failed inspection and can not be reworked. These happen on occasion
just have them hold one for you.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger





From daemon Fri Jan 19 06:07:19 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Fri, 19 Jan 2001 13:02:28 +0100
Subject: Antwort: LR White protocol for plant tissues

Contents Retrieved from Microscopy Listserver Archives
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Hi Rinaldo,

sorry that I answer your question a bit late! Some weeks ago you asked
about recipes for LR White embedding of plant tissue.

Well, here are some references:

Lonsdale J.E., McDonald K.L., Jones R.L. (1999): High pressure freezing and
freeze substitution reveal new aspects of fine structure and maintain
protein antigenicity in barley aleurone cells. Plant Journal 17(2):
221-229.

Takahashi, H., Kuroiwa, H., Miyagishima, S., Toda K., Itoh R., Kuroiwa T.
(1997): Improved procedure for immunogold electron microscopy: Rapid-freeze
substitution with absolute acetone. Cytologia (Tokyo) 62(3): 303 ? 308

This one is with LR Gold:
Staff, I.A., Schappi, G., Taylor P.E. (1999): Localisation of allergens in
ryegrass pollen and in airborne micronic particles. Protoplasma 208(1-4):
47 ? 57.

Hope that helps,

Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350



From daemon Fri Jan 19 08:15:02 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Fri, 19 Jan 2001 08:09:12 -0600
Subject: Problems with blue staining

Contents Retrieved from Microscopy Listserver Archives
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Hello
} My problem is: after revealing the semithin slices (10 um), I can see
} eye-naked the blue staining, but then it fades away in 10 to 30
} minutes. Can it be possible that the glycerol-PBS that I use as
} mounting media has something to do, or it is maybe the PBS in which I
} rinse the slides after revealing, to clean the excess and toxic DAB ?
}
} We suspect the PBS may have some contaminating elements which may
} reduce the DAB so it re-dissolves, or the glycerol is too old (it was
} bought in 1997) and has turned acid so it gives protons to the media
so
} DAB re-dissolves.
} Has it ever happened to anyone? Thank you.
}
} Albert Cardona.
} University of Barcelona. Spain.


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From daemon Fri Jan 19 08:20:25 2001



From: ldb2-at-ix.netcom.com
Date: Fri, 19 Jan 2001 08:16:27 -0600
Subject: Ask-A-Microscopist: Student Science Fair Project on DNA

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------------------------------

Email: ldb2-at-ix.netcom.com
Name: Larry Bell
School: Westfields High School
State: VA

Question:
My son built a polarized light microscope and has been trying to observe
the liquid crystal properies of DNA for his 10th grade science fair
project. We've been all over looking for books and materials on the
subject and haven't had much luck. We've seen great pictures of the 3
liquid crystal phases exhibited by DNA, but can't find information on
preparing the sample and the slides. His science teacher gave him a simple
procedure to extract DNA from peas (or bananas) using a blender, meat
tenderizer and alcohol. The DNA rises to the top after the alcohol is
added. He tried allowing a drop of DNA/Alcohol mixture evaporate under the
crossed polarizer, but no changes have been observed. I'm told he needs to
"dissolve the DNA in a buffered saline solution prior to evaporation". Can
you advise him on a simple process to prepare the sample, and offer advice
on how to obtain or make the buffered saline solution?

Can you help us with a simple process to prepare a DNA sample for analysis
by evaporation?

Any advice you can offer would be appreciated.



---------------------------------------------------------------------------




From daemon Fri Jan 19 09:35:41 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 19 Jan 2001 07:30:44 -0800 (PST)
Subject: RE: Freeze-Fracture replica cleaning

Contents Retrieved from Microscopy Listserver Archives
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I have used all of the aforementioned replica cleaning reagents. One that
hasn't been mentioned yet, and that is very good for cleaning Pt/C replicas
is HF. The method is like all the rest, except that the HF has to be in
plastic containers (small petri dish, usually). Cleaning time can be up to
overnight, wash by transferring across several water washes, and pick up on
the grid. This is the method of choice for high resolution
shadowed/replicas protein preps. I have used it on other specimens as well,
and think that if necessary you could try it on yours. Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Thu, 18 Jan 2001 21:57:52 -0700, Al Coritz wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Majid:
}
} The common cleaning methods for FF replicas include: Conc. Bleach, 70%
} Sulfuric acid, or 50%Chromic acid. These work for almost everything I
have
} encountered. For the others I recommend Fisher Scientific Glass Cleaning
} Solution*. Start with 10% increasing to 50% overnight in a humidified
} chamber with a cover.
}
} If you don't take care to slowly increase the conc. of ANY of the
cleaning
} solutions you take the chance of "cooking" the remaining biological
material
} to the replica. This maybe what is happening. Several labs I work with
are
} using Xenopis oocytes and don't encounter problems with the replicas and
} they use only Bleach. Plasma cleaning replicas may also "cook" the
replica
} too but I don't know for certain.
}
} I think the brittleness you are encountering is related strictly to the
} replication process and not the cleaning. I would need more information
on
} your protocol to comment on what might be causing it. Pt/C replicas are
} stabile in all of the solutions mentioned above for extended cleaning
times.
}
} * I have no financial interest in Fisher Chemicals or Products.
}
}
} Al Coritz
} Sales & Service Manager
} RMC-Boeckeler Instruments
} 4650 S. Butterfield Dr.
} Tucson, AZ 85714
} Voice: 520-745-0001
} Cell: 520-465-3598
} Fax: 520-745-0004
} Email:Al-at-Boeckeler.com
} Website:RMCProducts.com
}
}
}
} -----Original Message-----
} } From: Majid [mailto:majid.ghoddusi-at-anu.edu.au]
} Sent: Thursday, January 18, 2001 7:11 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Freeze-Fracture replica cleaning
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hello all
}
} We are preparing Pt-C and C replicas from different biological material.
For
} cleaning the obtained replica we either use bleach (5.2%) or chromic acid
} (10, 20 followed by 40%) with washes in between. The protocol usually
works
} well for most stuff but when it comes to oocytes from Xenopus we are
usually
} left with a lot of organic material still attached to the replica. If we
} leave the replica in cleaning agent for a long time we end up with a
very
} brittle replica which can easily break.
}
} Oocytes have got a reputation for being very sticky and difficult to
clean.
} We are considering to use plasma cleaning instead but I don't know what
to
} expect from that. I would appreciate it if you could share your 'tips and
} tricks' with us.
}
} Majid Ghoddusi
}
}
}
} ...............................................................
} Majid Ghoddusi, PhD
} Protein Dynamics Unit
} Department of Chemistry
} Australian National University
} ACTON 0200
} Canberra
} Tel: (02) 6125 4190
} Fax: (02) 6125 0760
} e.mail: majid.ghoddusi-at-anu.edu.au
} web site: http://langevin.anu.edu.au/
}
.........................................................................
} .........
}
}
}
}





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From daemon Fri Jan 19 09:53:38 2001



From: Stephen Skirius :      Stephen_Skirius-at-bkitech.com
Date: Fri, 19 Jan 2001 09:47:01 -0600
Subject: The preparation and examination of wood fibers by TEM

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Is there a "standard" procedure for the embedding, sectioning and staining
of wood fibers for examination by transmission electron microscopy?

Steve


From daemon Fri Jan 19 09:57:27 2001



From: Tom Pella :      tom_pella-at-tedpella.com
Date: Fri, 19 Jan 2001 07:53:35 -0800
Subject: Re: Negatives-Need help find clear plastic sleeves

Contents Retrieved from Microscopy Listserver Archives
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We sell clear polypropylene sleeves, 3 1/4 x 4, product number 35341-P.

Ted Pella, Inc.
800-237-3526

Tom Pella




From daemon Fri Jan 19 10:16:43 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 19 Jan 2001 08:13:00 -0800 (PST)
Subject: Re: LM: tongue paraffin embedding help

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Sue:

First, a question: are you embedding the whole tongue or a cross section (or
cross sections)? We routinely process rat tongue as part of the tissue
workup on whole animal collections. We collect a cross section
approximately 7mm long and embed to view the cross section. Processing is
done with the same schedule that we use for all the rest of our rat tissue.
Dehydration/clearing are at 45 min steps except for the last 100% alcohol
and clearant (those are 60 min each). We use either two 1 hour 30 min steps
in paraffin (Shandon Hypercenter XP) or two 45 min and two 60 min steps (VIP
E300). The only differences I can see between the two processors is that
the skins and cns tissues tend to be less well embedded through the Shandon
two-stage paraffin vs the VIP four-stage paraffin. Tongue embeds equally
well in either (as does other dense tissues)--the problem with skin
(especially from female rats or very old animals--either sex) and cns is the
high lipid/fat content and the ability to remove/replace that more
effectively with the additional paraffin infiltration steps. The other
thing I do is to make sure that my paraffins are not contaminated with
clearant (even the first stage). If the paraffin feels "slippery" when
rubbed between finger and thumb, dump it and replace with fresh paraffin.
Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Thu, 18 Jan 2001 16:15:54 -0500, Sue Hendricks wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone offer any tips for embedding rat tongue tissue in paraffin?
I
} am having difficulty with complete infiltration (?) of the tissue. The
} tongue has a dense muscle core that becomes hard during the process
causing
} shredding during sectioning. Any help would be much appreciated.
}
} On a second note: This tissue has been injected with tritiated
thymidine.
} Has anyone ever heard of loss of radioactive label during tissue post-fix
or
} embedding?
}
} thanks so much,
}
} Susan Hendricks
} University of Virginia
} Department of Psychology
} PO Box 400400
} Gilmer Hall
} Charlottesville, VA 22904
}
} 804.982.4755
} 802.982.4785 [fax]
} sjh2e-at-virginia.edu
}
}





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From daemon Fri Jan 19 10:55:17 2001



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Fri, 19 Jan 2001 17:33:08 -0000
Subject: Sputter coater

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Thanks, Allen, for pointing out some of the issues here. By the way, It's
"Wehnelt", not "Wienault".

You are probably right about the ESD vs. EDS name. However, it was clear to
me what the original question was about.

Someone pointed out, that the cause of ESD (!) failure in electronic devices
is, that the fields increase to beyond the breakdown strength of the
devices, which causes a high and sudden current to flow through the parts
where the fields are highest, which in turn heats and destroys the sample. I
think, that is right. It's the fields that reach critical strength, which
then causes the current flow and destruction of the device. That's what I
meant when I said you get "zapped".

Now, about the electron microscope. Obviously I don't want to pronounce,
that electron microscopy is impossible. That would be stupid, wouldn't it?
However, as you said yourself, the sample is at ground potential, or very
near it, and so is the anode. In other words, there is not electric
potential (or very little) between the anode and the sample and thus no
electric field. Since the sample is grounded, as is the rest of the
microscope, there is no electric field between those, either. The energy of
an electron in an electric field is proportional to the electric field
(actually proportional to the square of the field, if my memory serves me).
No field - no electric energy. Of course that does not mean that the
electrons don't have energy. They have quite a lot of energy, on the order
of 20keV, by "falling" through the electric field between cathode and anode
and not being stopped by the anode. Since the sample is grounded (or should
be), the electric effects of the electron beam on the grounded sample will
be cancelled. What you have to contend with is the kinetic energy (20
keV/electron), which is transferred to the sample.

Now, I have always talked about grounded samples. That does not mean that
electric fields cannot be generated by the electron beam within the sample.
Obviously, you put electrons in the sample and they have to move out of the
sample. If there is any kind of resistance (an oxide layer, etc.), that will
result in charging and an electric potential.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Allen R. Sampson [mailto:ars-at-sem.com]
Sent: Friday, January 19, 2001 2:44 AM
To: 'Mike Bode'


Hi,
Many thanks for the suggestions.
We have an Oxford Instruments 1500 attached to an SEM. Oxford themselves
say that this model will only sputter Au or Au/Pd, although they supply all
types of target. It can be upgraded to a 1500HF at £11,000 plus tax which
will do Pt but they say we would also need to swap the rotary vacuum pump
for a turbo. We compromised and bought an Au/Pd target. We find cracking
open the valve to the SEM chamber to get a sniff of the higher vacuum helps
deposition.
ttfn, Chris


From daemon Fri Jan 19 11:38:18 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 19 Jan 2001 12:35:49 -0500
Subject: Embedding samples after GUS staining

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Microscopists,
We have some students doing projects where GAS staining is used as a genetic marker in arabadopsis roots. These roots are very small and must then be fixed embedded and thick-sectioned so that the distribution of the blue GUS staining can be observed with the light microscope.

The staining tends to fade badly with most embedding procedures. We have found that if samples are only run up to 95% ETOH and then embedded, the staining intensity is preserved. Thus only resins which tolerate 5% water can be used. We have successfully used technovit but this resin is very difficult to cut. Has anyone had experience with this stain and other resins that might preserve the stain intensity but is easier to handle (and hopefully not as expensive!).
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907





From daemon Fri Jan 19 15:58:35 2001



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Fri, 19 Jan 2001 12:51:43 -0900
Subject: Used fiber optic panel for Zeiss 109

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Fellow Listers,
Our Zeiss 109 recently developed a large vacuum leak in the fiber optic
panel (Between the column and the camera). I am told a new one runs
approximately $6000.00. We are no longer on service contract, but my old
service technician at LEO may already have a line on a used one. Above and
beyond the call of duty, if you ask me. In case that falls through I
thought I'd see If anyone out there might know of a likely source.
Thanks in advance
You are all so amazingly resourceful

Kim DeRuyter
Histology and Electron Microscopy Technician
900 Yukon Dr.
Fairbanks, Alaska 99775-0180
907-474-5452





From daemon Fri Jan 19 16:36:16 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Sat, 20 Jan 2001 09:32:13 +1100
Subject: Re: Used fiber optic panel for Zeiss 109

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Kim,
I think there is a 35mm camera port on that microscope? You might be able
to blank off the bottom plate and pick up a second hand 35mm camera.
Good luck
Sally




Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218 or 6125 8525
http://www.anu.edu.au/EMU


} } } Kim DeRuyter {fnksd1-at-uaf.edu} 01/20/01 08:51am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fellow Listers,
Our Zeiss 109 recently developed a large vacuum leak in the fiber optic
panel (Between the column and the camera). I am told a new one runs
approximately $6000.00. We are no longer on service contract, but my old
service technician at LEO may already have a line on a used one. Above and

beyond the call of duty, if you ask me. In case that falls through I
thought I'd see If anyone out there might know of a likely source.
Thanks in advance
You are all so amazingly resourceful

Kim DeRuyter
Histology and Electron Microscopy Technician
900 Yukon Dr.
Fairbanks, Alaska 99775-0180
907-474-5452






From daemon Fri Jan 19 18:18:18 2001



From: Jim_Koncki-at-Ingersoll-Rand.com
Date: Fri, 19 Jan 2001 18:14:00 -0600
Subject: Carbon in Steel Standards for WDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:

I need to obtain some certified standards for
carbon in steel matrix to do quantitative work using WDX.
The problem is finding material that is homogeneous
on a micro-scale. I am trying to cover a range of 0.1 to
2.0 Wt. % carbon. No luck thus far with NIST or Brammer.

Does anyone know of a supplier who can produce
and sell such material?

Thanks in advance.

Jim Koncki
Senior Project Chemist
Torrington Co./Ingersoll-Rand

860-626-2774
fax 496-3636




From daemon Fri Jan 19 18:18:18 2001



From: Garone, Lynne C :      GARONEL-at-polaroid.com
Date: Fri, 19 Jan 2001 18:12:16 -0600
Subject: Alden printer drivers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone use an Alden model 9315 CTP direct thermal printer to print
images? We have obtained this printer without drivers and the company
appears to be out of business.
Thanks,
Lynne C. Garone
Polaroid Corp.
W4-1D
1265 Main St.
Waltham, MA 02451-1714
(781) 386 -1446
(781) 386 -0378
GaroneL-at-Polaroid.com




From daemon Fri Jan 19 19:14:02 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 19 Jan 2001 19:10:58 -0600
Subject: Re: Used fiber optic panel for Zeiss 109

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Kim DeRuyter" {fnksd1-at-uaf.edu}
} Fellow Listers,
} Our Zeiss 109 recently developed a large vacuum leak in the fiber optic
} panel (Between the column and the camera). I am told a new one runs
} approximately $6000.00. We are no longer on service contract, but my
old
} service technician at LEO may already have a line on a used one. Above
and
} beyond the call of duty, if you ask me. In case that falls through I
} thought I'd see If anyone out there might know of a likely source.
} Thanks in advance
} You are all so amazingly resourceful
}
A free wanted add on www.labx.com turns up a lot of hard to find stuff and
the price it right.

I have no connection with labx except using their service to find some
really hard to find stuff.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger





From daemon Fri Jan 19 19:54:53 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sat, 20 Jan 2001 11:51:50 +1000
Subject: RE: Metal for Sputter Coating Targets -- Thanks and a summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that the emphasis is not quiet right. If you have resolution problems
due to the coating in the common SEM magnification range at say up to x60k,
chances are, its the thickness of the coating and not the grain-size.
The analogy with a blanket of snow should be clear to all of you in frigid
climates: A dusting of snow contours every pebble, whereas a 300mm layer would
hide all small features. You cannot expect to see much detail using a thick
coating.
A thin coating may not be continuos because of grain size and a thin coating
may lead to charging effects and these have to be addressed by various means.
Finer grain size gives a more continuous coating, but if the coating is thick
the grain-size scarcely matters in terms of resolution.
The difference in grain size between Au and Au/Pd is marginal and was first
given as a theoretical advantage when SEM specimens were coated by evaporation.
Sputtering gives lower grain sizes than evaporation for any given metal, but I
guess that the relative grain-size remains.
I rather doubt that you will find any real advantage after switching from Au to
Au/Pd targets. Pt would give an advantage at high SEM mags, but even then, part
of the effect may be the thinner coating that Pt gives when compared with Au.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, January 19, 2001 7:29 AM, Michael D. Standing
[SMTP:Michael_Standing-at-byu.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers:
}
} Thank you for the numerous responses to my query about which metals and
} operating conditions to consider for improving the grain size of the
} coatings we have been getting from our sputter coater. Below is a brief
} summary of the recommendations we have received. I will be experimenting
} with several of these items to see where we ultimately want to end up with
} this. I suspect that we will develop several protocols based on the
} requirements of the experiment. Again thank you.
}
} 1) Gold/Palladium definitely has a smaller grain and we should see a
} definite improvement by using it.
}
} 2) Platinum will have the finest grain size but it takes longer to deposit,
} potentially causing damage to sensitive samples.
}
} 3) Platinum/Palladium is sometimes used as an alternative to Chromium
} coating
}
} 4) Reduce the coating thickness as much as possible
}
} 5) Coat using higher vacuum / lower currents / lower voltage to reduce grain
} size
}
} 6) Pulse the deposition
}
} 7) Decrease sample surface temperature
}
} 8) Gold may be the best alternative for samples where resolution is not as
} much a factor as is sample integrity as the other metals require more energy
} / longer exposure to get an adequate coating.
} ======================================
} Michael D. Standing
} BYU Microscopy Lab
} 401 WIDB
} Brigham Young University
} Provo, UT 84602
}
} e-mail: Michael_Standing-at-byu.edu
} phone: (801) 378-4011
} fax: (801) 378-3937
} ======================================
}
}



From daemon Sat Jan 20 07:29:03 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Sat, 20 Jan 2001 05:22:21 -0800 (PST)
Subject: RE:RE:Problems with Blue staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Grace
It was my first question here and it amazes me how fast answers came
from everywhere.
Yes, I'm using niquel amonium sulfate as DAB enhancer, and then I mount
the slides with glycerol-PBS 50%, since the slides are 8-well ones
-pretty expensive- and we re-use them hundreds of times. It would not
be possible to do so with permanent mounting media as DPX or permount.
Anyway, there is no point in keeping the screening results ... these
immunostainings are used screen monoclonal antibodies produced by
myself.
But, the issue is, it has always been possible to mount the slides in
glycerol-PBS with no loss-of-stain problems -at least, the blue stain
lasted 2 days. But now we are losing the staining in 30 minutes, even
less, sometimes even 10 minutes. That is why I was asking if the
glycerol aged more acid had something to do, or the PBS.
Thank you.
Oh, by the way, I accidentaly deleted the last 10 mails, one of them
also including an answer to my request. If anybody could be so kind as
to resend it to me to planaria2000-at-yahoo.com I would really appreciate
it.

Albert Cardona
University of Barcelona. Spain.

__________________________________________________
Do You Yahoo!?
Yahoo! Auctions - Buy the things you want at great prices.
http://auctions.yahoo.com/


From daemon Sat Jan 20 07:56:55 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Sat, 20 Jan 2001 05:53:57 -0800 (PST)
Subject: RE: RE: Problems with blue staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Grace
It was my first question here and it amazes me how fast answers came
from everywhere.
Yes, I'm using niquel amonium sulfate as DAB enhancer, and then I mount
the slides with glycerol-PBS 50%, since the slides are 8-well ones
-pretty expensive- and we re-use them hundreds of times. It would not
be possible to do so with permanent mounting media as DPX or permount.
Anyway, there is no point in keeping the screening results ... these
immunostainings are used screen monoclonal antibodies produced by
myself.
But, the issue is, it has always been possible to mount the slides in
glycerol-PBS with no loss-of-stain problems -at least, the blue stain
lasted 2 days. But now we are losing the staining in 30 minutes, even
less, sometimes even 10 minutes. That is why I was asking if the
glycerol aged more acid had something to do, or the PBS.
Thank you.
Oh, by the way, I accidentaly deleted the last 10 mails, one of them
also including an answer to my request. If anybody could be so kind as
to resend it to me to planaria2000-at-yahoo.com I would really appreciate
it.

Albert Cardona
University of Barcelona. Spain.

__________________________________________________
Do You Yahoo!?
Yahoo! Auctions - Buy the things you want at great prices.
http://auctions.yahoo.com/


From daemon Sat Jan 20 08:35:36 2001



From: Markus F. Meyenhofer :      micro-at-mail.superlink.net
Date: Sat, 20 Jan 2001 08:30:24 -0600
Subject: Help for LKB Ultrotome V

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all.
I would like to get a circuit diagram for an LKB Ultrotome V (2088)
Ultra Microtome. Please contact me direct.
Thanks for any help.
Markus




From daemon Sat Jan 20 20:53:40 2001



From: Tim Richardson :      mirlyn-at-attglobal.net
Date: Sat, 20 Jan 2001 21:31:52 -0500
Subject: Food Structure and Functionality Symposium 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For your information, thought you all might be interested to know what is
happening in microscopy in this sector. The sender is the head of plant /
food em microscopy at AG Canada in Dartmouth NS I think.

Tim

=========

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Food Structure & Functionality Symposium 2001
An international symposium leading food structure & Functionality studies
through the 21st century
*webaddress at the AOCS site
http://www.aocs.org/member/division/fsff/index.htm*

Being held in conjunction with the , May 13-16, 2001, Minneapolis
Convention Center, Minneapolis, Minnesota, USA. For information, e-mail us
at: meetings-at-aocs.org

The symposium has two themes:
* New and novel approaches (including microscopy, rheology and
spectroscopy) to the study of structure-function relationships in foods;
* Food system studies covering any part of the processing chain - from the
raw material to the final product, and including trouble shooting.

Tentative Program (confirmed as of January 12th, 2001)

May 13th - Short Courses (short courses will run for a full day, and will
run concurrently)

1) Food Contaminants. Contact person - Mark Auty
2) Specific Labeling in Foods. Contact person - Marcel Paques

May 14th-16th inclusive - Technical sessions
6 sessions will run over 3 days

Morning
Symposium opening
Plenary lecture - Food Quality andwhy the Structure matters. P. Lillford,
Unilever Research, Colworth House UK

Session 1: Dairy Products and Fat Based Foods Session - chairs M. Auty and
M. Paques

Milk protein polysaccharide interactions in aqueous solutions and
oil-in-water emulsions.
H. Singh*, Y. Hemar & P. Munro, Institute of Food, Nutrition and Human Health
Massey University, Palmerston North, New Zealand

Structural functions of dairy ingredients in products formulated with
taro flour.
C. Onwulata, USDA/ARS,Eastern Regional Research Center,Wyndmoor, PA 19038

Confocal imaging of galactomannan mode of action in recrystallisation of
ice in model ice cream
D. Ferdinando, Unilever Research Colworth House, UK

Heating of Food Proteins in a Closed System at High Temperature
N. Kitabatake, Kyoto University, Japan

Milk Protein - molecular components and functional properties. N. C.
Ganguli, Indian Dairy Association

Afternoon
Session 2: Food Safety - chairs J. W. Arnold and R. Droleskey
Prevention of Foodborne Illness Through Sanitation and Processing Technology
J.W. Arnold; USDA/ARS, Russell Research Center; Athens, GA 30604

PreHarvest Intervention Strategies to Control Foodborne Pathogens in Poultry
J. A. Byrd; USDA/ARS/SPARC; College Station, TX 77845

Interactions of Competitive Exclusion Cultures with the Intestinal Mucosa
of Newly Hatched Chicks
R. Droleskey; USDA/ARS/SPARC; College Station, TX 77845

Adhesion of Salmonella on Alfalfa Sprouts
A. Chartowski; USDA/ARS, Western Regional Research Center; Albany, CA 94710

Growth of Fusarium moniliforme Dependent upon Corn Tissue Type
I. E. Yates; USDA/ARS, Russell Research Center; Athens, GA 30604

Dedicated poster viewing 4:00-6:00PM

Evening: Round Table Discussion - topic to be announced


Tuesday, May 15th, 2001
Morning
Session 3: New Methods and Techniques for Food Structure and Functionality
Analysis -chair K.Groves

Diffusing wave spectroscopy - a new and non-invasive method for the
investigation of the structure, dynamics and interactions in complex food
systems
M. Alexander* and P. Schurtenberger

Food: How complex can it be?
E. Esselink ,Unilever Research Vlaardingen, The Netherlands

Immunolocalization of Transgenic Protein in Wheat Endosperm
M. L. Parker *, E. Stoger, R. Casey and P. Christou, Institute of Food
Research, Norwich, England.

Structure/Function relationships through microrheology.
M. Paques, Wageningen Centre for Food Sciences/Unilever Research Vlaardingen

Specific labeling techniques for foods
J. Leunissen, Aurion: Immuno-Gold Reagents & Accessories, The Netherlands


Food Structure and Functionality Division Luncheon. Dr. Brian Brooker
(Institute of Food Research, England * retired) will be presented with the
Food Structure and Functionality Division award and will give a
presentation entitled: Fat crystals - the importance of
being small.

Afternoon
Session 4: Agricultural Applications of Microscopy and Imaging Session-
chairs D.F.Wood and P. Allan-Wojtas

The Utility of Sorting in Agriculture.
H. J. Arnott, Department of Biology, University of Texas at Arlington, Texas

The Potential for Automatic X-ray Sorting of Insect Infested Grain
R. Haff . USDA - ARS - WRRC, Albany, California.

Automated Sorting of Almonds with Embedded Shell by Laser Transmittance
Imaging
T. Pearson* R. Young, USDA - ARS - WRRC, Albany, California.

Use of a GFP-transformed strain of Fusarium graminearum to study ear rot
development in corn
S. S. Miller. , AAFC - ECORC, Ottawa, Ontario, Canada.

Popping modifies endosperm structure and improves digestibility in maize
and sorghum.
M.L. Parker, Institute of Food Research, Norwich, England.

Microstructural Changes in Rice During Cooking
D. Wood* and P.C. Yu . USDA - ARS - WRRC, Albany, California.

Evening: Food Structure and Functionality Division Member meeting

Wednesday, May 16th, 2001
Morning
Session 5: Ingredients and Food Processing - chairs D. Kittleson and J.
Charbonneau

Water continuous fat crystal networks in ice cream induced by unsaturated
monoglycerides
N. M. Barfod , Danisco Cultor, Brabrand, Denmark

High pressure application fo food systems and its impact on functional
ingredients
B. Tauscher*, P. Butz,and A.F. Garcia, Federal Research Center for
Nutrition, Karlsruhe, Germany

Specificity and application of lipolytic enzymes in bread making processes
T.B. Frandsen, T. Spendler, G. Budolfsen, L. Christiansen and J. B.
Neilsen, Novozymes A/S, Bagsvaerd, Denmark

Afternoon
Session 6: Colloidal and Interfacial Sciences - chairs D. Pechak and M. Paques

Rheology and Structure of Particulate Protein Gels
T. van Vliet, Wageningen Centre for Food Sciences,Wageningen, The Netherlands

Posters:

1. In situ study of the effect of heating on dough components using
Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR)

O. Sevenou, S.E. Hill, I.A. Farhat and J.R. Mitchell. Division of Food
Sciences, University of Nottingham, Loughborough, UK

2. Antioxidative activity of the crude extract of lignan glycosides from
sesame meal

Y-S Shue and L.S. Hwang, Graduate Institute of Food Science and Technology,
National Taiwan University, Taiwan, R.O.C.

3. Near Field Microscopy of phase separation in a mixed interfacial
protein/surfactant film

A. P. Gunning, A.R. Mackie, A.R. Kirby and V.J. Morris, Institute of Food
Research, Norwich Research Park, Norwich, UK

Contact information for the chairs is shown below, in alphabetical order:

Paula Allan-Wojtas
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre, Kentville, Nova Scotia,
Canada
B4N 1J5
Tel: (902) 679-5566
FAX: (902) 679-2311
email: allanwojtasp-at-em.agr.ca

Judy Arnold
USDA -ARS - RRC
950 College Station Rd.
P.O. Box 5677
Athens, GA 30604-5677
USA
Tel: (706) 546-3515
FAX: (706) 546-3068
email: jarnold-at-ars.usda.gov

Mark Auty
Dairy Products Research Centre
TEAGASC
Moorepark, Femoy, Co. Cork
Ireland
Tel: 011-353-25-42447
FAX: 011-353-25-42340
email: mauty-at-moorepark.teagasc.ie

James E. Charbonneau
National Food Processors Association
Food Chemistry and Packaging Department
1401 New York Ave, NW
Washington, D.C. 20005
USA
Tel: (202) 639-5972
FAX: (202) 639-5991
email: jcharbo-at-nfpa-food.org

Kathy Groves
Leatherhead Food Research Association
Randalls Road, Leatherhead
Surrey KT22 7RY
England
Tel: 44 0132 822330
FAX: 44 0132 386228
email: kgroves-at-lfra.co.uk

Diana Kittleson
Pillsbury TPC Labs
737 Pelham Blvd.
St. Paul, MN 55114
USA
Tel: (651) 917-5859
FAX: (651) 917 5850
email: dkittleson-at-pillsbury.com

Tony McKenna
New Zealand Dairy Institute
Private Bag 11 029
Palmerston North,
New Zealand
Tel: 011 64 6 350 4649
FAX: 011 64 6 356 1476
email: tony.mckenna-at-nzdri.org.nz

Marcel Paques
Wageningen Centre for Food Sciences/Unilever Research Vlaardingen
P.O. Box 20, 6710 BA Ede
The Netherlands
Tel: 011 31 318 659690
FAX: 011-31-318 650400
email: paques-at-nizo.nl

David Pechak
Kraft Technology Centre
801 Waukegan Road
Glenview, IL 60025
USA
Tel: (847) 646-4808
FAX: (847) 646-3864
email: dpechak-at-kraft.com

Delilah Wood
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5653
FAX: (510) 559-5777
email: wood-at-pw.usda.gov



===============================================================
Tim Richardson, R&D, Bio-Microtech Inc. & Bolton Bio-Research
email: mirlyn-at-attglobal.net, web: www.bio-microtech.com
ph: 905-951-7058 fax: 905-951-7052
4-670 Hardwick Road, P.O. Box 23, Bolton, Ontario, L7E 5T1, Canada





From daemon Sun Jan 21 11:31:33 2001



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Sun, 21 Jan 2001 10:21:06 -0700
Subject: Carbon in Steel Standards for WDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might try Geller Microanalytical Lab in Peabody, Mass. The last phone
number I had for him was 508-535-5595. If he doesn't have it, he would know
where to direct you.

Good Luck.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Jim_Koncki-at-Ingersoll-Rand.com"-at-sparc5.microscopy.com
[mailto:"Jim_Koncki-at-Ingersoll-Rand.com"-at-sparc5.microscopy.com]
Sent: Friday, January 19, 2001 5:14 PM
To: Microscopy-at-sparc5.microscopy.com


Hi All:

I need to obtain some certified standards for
carbon in steel matrix to do quantitative work using WDX.
The problem is finding material that is homogeneous
on a micro-scale. I am trying to cover a range of 0.1 to
2.0 Wt. % carbon. No luck thus far with NIST or Brammer.

Does anyone know of a supplier who can produce
and sell such material?

Thanks in advance.

Jim Koncki
Senior Project Chemist
Torrington Co./Ingersoll-Rand

860-626-2774
fax 496-3636




From daemon Mon Jan 22 00:48:06 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Mon, 22 Jan 2001 07:35:39 +0100
Subject: Antwort: The preparation and examination of wood fibers by TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi Stephen,

there is (probably not a "routine method") a really good paper on embedding
Cornus wood in Spurr:

Ristic, Z., Ashworth E.N. (1995): Response of xylem ray parenchyma cells of
supercooling wood tissues to freezing stress: Microscopic study.
International Journal of Plant Sciences. 156(6): 784-792.


The preparation there was done by plunge freezing followed by Freeze
Substitution.

Hope that helps,

Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350



From daemon Mon Jan 22 02:21:05 2001



From: Labar Janos :      labar-at-mfa.kfki.hu
Date: Mon, 22 Jan 2001 10:41:58 +0100
Subject: TEM of Mg-based alloys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

A friend wants to examine Mg-based alloys in TEM and warned us that in a
previous lab this alloy was expelled from the microscope because it caused
contamination by sublimation. I have no experience with these materials. Can
anyone give me more detailed info if this is a real danger?

Thanks in advance:

János


Dr. habil, Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar




From daemon Mon Jan 22 05:21:35 2001



From: Giles Sanders :      g.sanders-at-ic.ac.uk
Date: Mon, 22 Jan 2001 12:09:09 -0000
Subject: Interfacing digital camera to microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, please forgive me. My spelling is not very accurate when I am
responding so early in the morning. Your correction on Wehnelt is correct.
Please forgive any such errors in this response, as it is similar in
timing.

The electric field of interest is not between any parts of the column, but
rather between the electrons and the sample. I really don't want to try to
attempt any quantum exercises here, but the energy impressed on the
electrons results in their increased EM field effects. The acceleration of
the electrons controlled by the electron gun configuration controls the
energy afforded the electrons. What happens after the anode does not
affect the energy of the electrons in the beam.

The electrons are the field. The acceleration given to them by the gun
determine their acceleration, and thus their field and relativistic
effects. Their energy is determined by the fields in the gun structure
alone. Once they are given that energy, their travel through the column is
determined by the momentum they have been given. The fields they generate
as they travel through the sample surface are the direct result of the
energy they were provided with in the electron gun.

Here's a simple challenge - define the 'kinetic' energy of a fast moving
electron. As the kinetic energy is generally defined as the mass vs. the
velocity of that mass, you may have a problem. The best high energy
experiments have been unable to assign a 'mass' to the electron.
Apparently, the only mass associated with the electron is the Einsteinium
energy-mass equivalent of its charge. If there is any increase in an
electron's mass/energy by acceleration in an applied electric field, it is
to the electrons energy. Check with SLAC's experiments.

A fine point, but one with merit. It doesn't matter whether the distance
from anode to sample is 10 cm or 1000 cm, if the electron can travel the
distance without interference and external force. Thus the electro-magn
etic interaction of the electron with the sample is simply dependant on the
force originally impressed on the electron by the gun.

The field present at the sample surface will thus be determined by the
acceleration given the electrons by the gun and their current density. The
higher the beam current, the greater the field flux. The smaller the area
of the electron impingement on the surface, the higher the field flux. As
we work towards smaller circuit dimensions, these electron interactions
produce higher field flux densities as the circuit dimensions come closer
to the electron beam diameters.

You seem to want to separate the mass related kinetic effects from the
energy of the particle, but that can not be done. As the electron is
accelerated, its energy is apparently increased, rather than its mass. The
result is that the classical and relativistic effects normally related to
mass are instead seen in an increase in the energy, and thus, the field
effects of the electron with the sample.

On Friday, January 19, 2001 10:43 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
wrote:
} { { File: ATT00000.txt; charset = windows-1252 } }

Thanks, Allen, for pointing out some of the issues here. By the way, It's
"Wehnelt", not "Wienault".

You are probably right about the ESD vs. EDS name. However, it was clear to
me what the original question was about.

Someone pointed out, that the cause of ESD (!) failure in electronic
devices
is, that the fields increase to beyond the breakdown strength of the
devices, which causes a high and sudden current to flow through the parts
where the fields are highest, which in turn heats and destroys the sample.
I
think, that is right. It's the fields that reach critical strength, which
then causes the current flow and destruction of the device. That's what I
meant when I said you get "zapped".

Now, about the electron microscope. Obviously I don't want to pronounce,
that electron microscopy is impossible. That would be stupid, wouldn't it?
However, as you said yourself, the sample is at ground potential, or very
near it, and so is the anode. In other words, there is not electric
potential (or very little) between the anode and the sample and thus no
electric field. Since the sample is grounded, as is the rest of the
microscope, there is no electric field between those, either. The energy of
an electron in an electric field is proportional to the electric field
(actually proportional to the square of the field, if my memory serves me).
No field - no electric energy. Of course that does not mean that the
electrons don't have energy. They have quite a lot of energy, on the order
of 20keV, by "falling" through the electric field between cathode and anode
and not being stopped by the anode. Since the sample is grounded (or should
be), the electric effects of the electron beam on the grounded sample will
be cancelled. What you have to contend with is the kinetic energy (20
keV/electron), which is transferred to the sample.

Now, I have always talked about grounded samples. That does not mean that
electric fields cannot be generated by the electron beam within the sample.
Obviously, you put electrons in the sample and they have to move out of the
sample. If there is any kind of resistance (an oxide layer, etc.), that
will
result in charging and an electric potential.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Allen R. Sampson [mailto:ars-at-sem.com]
Sent: Friday, January 19, 2001 2:44 AM
To: 'Mike Bode'


Apologies in advance if this has been asked before ..

Has anyone had an success in interfacing an Epson PhotoPC 3000 digital
camera to an optical microscope?

Thanks

Giles Sanders
----------------------------------------------------------------------------
---------------------------
Dr. Giles Sanders
Zeneca / SmithKline Beecham Centre for Analytical Sciences
Chemistry Department
Imperial College of Science, Technology and Medicine
London
SW7 2AY

Web: http://www.achem.ic.ac.uk/gsanders/web/giles.htm

Tel: (44) - 020-7594-5749
Fax: (44) -020-7594-5833



Never express yourself more clearly than you think.
-- Niels Bohr (1885-1962) Danish physicist

----------------------------------------------------------------------------
------------------------



From daemon Mon Jan 22 06:41:10 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Mon, 22 Jan 2001 04:37:28 -0800 (PST)
Subject: LM - problems with paraffin, xylene and AA

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Hello everybody
My questions are:
1- Is it very important to use an extremly pure ethanol (AA) to remove
the xylene from the semithin sections placed on slides?
2- Can the tissue burst and the cells disrupt if it is not 100% pure?
3- Can the xylene stay within the tissue if the AA is not 100% pure,
as little yellow bubbles?
4- What happens if the paraffin has been stored at 56 degrees for too
long? Can it turn into longer polymers and then be harder or impossible
to remove from the sections? How may I detect so in the sections, is it
visible?

Albert Cardona
University of Barcelona, Spain.

__________________________________________________
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From daemon Mon Jan 22 09:06:09 2001



From: Louise_Harner-at-albint.com
Date: Mon, 22 Jan 2001 10:01:00 -0500
Subject: Carbon in Steel Standards for WDX

Contents Retrieved from Microscopy Listserver Archives
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Just thought I'd provide a corrected phone number & address (having been to
their lab). These were pulled off their website, but I didn't check to see if
they have what you're looking for.

Joseph D. Geller
Email: jg-at-gellermicro.com
www.gellermicro.com

Geller MicroAnalytical Laboratory
426e Boston St.
Topsfield, MA 01983-1212

Phone:(978) 887-7000
Fax:(978) 887-6671

Hope this helps. Disclaimer - I have no connection with Geller MicroAnalytical
Laboratory except as a satisfied customer. - Louise

Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com


----- Forwarded by Louise Harner/AlbInt on 2001/01/22 09:32 AM -----

"Ekstrom, Harry"
{harry.ekstrom-at-hone To: '\"Jim_Koncki {"'\"Jim_Koncki-at-Ingersoll-Rand.com\"
ywell.com} -at-sparc5.microscopy.com'" {"Jim_Koncki-at-Ingersoll-Rand.com"
-at-sparc5.microscopy.com} , Microscopy-at-sparc5.microscopy.com
2001/01/21 12:21 PM cc:
Subject: RE: Carbon in Steel Standards for WDX





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You might try Geller Microanalytical Lab in Peabody, Mass. The last phone
number I had for him was 508-535-5595. If he doesn't have it, he would know
where to direct you.

Good Luck.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Jim_Koncki-at-Ingersoll-Rand.com"-at-sparc5.microscopy.com
[mailto:"Jim_Koncki-at-Ingersoll-Rand.com"-at-sparc5.microscopy.com]
Sent: Friday, January 19, 2001 5:14 PM
To: Microscopy-at-sparc5.microscopy.com


Hi All:

I need to obtain some certified standards for
carbon in steel matrix to do quantitative work using WDX.
The problem is finding material that is homogeneous
on a micro-scale. I am trying to cover a range of 0.1 to
2.0 Wt. % carbon. No luck thus far with NIST or Brammer.

Does anyone know of a supplier who can produce
and sell such material?

Thanks in advance.

Jim Koncki
Senior Project Chemist
Torrington Co./Ingersoll-Rand

860-626-2774
fax 496-3636







From daemon Mon Jan 22 09:56:11 2001



From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 22 Jan 01 09:46:33 -0800
Subject: Re: TEM of Mg-based alloys

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Dear János,
I did a lot of TEM work of Mg-based alloys and I never experienced any
danger of these alloys in the microscope. The Mg matrix is definitely stable
in the microscope under the beam. The only concern may arise from the
stability of the compounds in your samples (usually I don't think it's a big
deal, since once a particle decomposes under the beam, the amount is quite
tiny). Anyway you may test your sample out of the microscope first.
Best regards,
Z.P. Luo

* * * * * * * * * * * * * * * * * * * * * *
Zhiping LUO
Materials Science Division, Building 223
Argonne National Laboratory
9700 South Cass Avenue, Argonne, IL 60439
Phone: (630) 252-8762
Fax: (630) 252-7777
E-mail: luo-at-msd.anl.gov
* * * * * * * * * * * * * * * * * * * * * *


} From: "Labar Janos" {labar-at-mfa.kfki.hu}
} To: "Microscopy ListServer" {Microscopy-at-sparc5.microscopy.com}
} Subject: TEM of Mg-based alloys
} Date: Mon, 22 Jan 2001 10:41:58 +0100
}
}
} Dear Colleagues,
}
} A friend wants to examine Mg-based alloys in TEM and warned us that in a
} previous lab this alloy was expelled from the microscope because it
caused
} contamination by sublimation. I have no experience with these materials.
Can
} anyone give me more detailed info if this is a real danger?
}
} Thanks in advance:
}
} János
}
}
} Dr. habil, Janos L. Labar
} Research Institute for Technical Physics and Materials Science
} H-1121 Budapest, Konkoly-Thege u. 29-33
} Postal address: H-1525 Budapest-114, Po Box 49
} Tel: (36)(1) 392-26-92
} Fax: (36)(1) 275-49-96
} Fax/phone: (36)(1) 395-92-32
} home page: www.mfa.kfki.hu/~labar
}



From daemon Mon Jan 22 10:16:29 2001



From: Colleen Genadry :      cgenadry-at-US.ibm.com
Date: Mon, 22 Jan 2001 11:12:12 -0500
Subject: CLEAR PLASTIC SLEEVES

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Importance: Normal


Dear Cindy,

The following is our supplier for clear "glassine " envelopes:

National Graphic Supply
226 N. Allen Street
Albany, NY 12206
Phone: (800)-223-7130

The manufacturer is Savage Universal Corporation in Arizona. We
have purchased, in the past, the following sizes:

2 3/4" X 3 3/4" Part No. SA 64 OPEN END (1000 per carton) - $
63.00 per carton*
4 1/4" X 5 1/4" Part No. SA 66 OPEN END (1000 per carton) - $
49.40 per carton**
5 1/4" X 7 3/8" Part No. SA 67 OPEN END (1000 per carton) -
$111.45 per carton*
8 1/4" X 10 1/4" Part No. SA 68 OPEN END (500 per carton) -
$102.90 per carton*

(Please NOTE: *last price - September, 1999; **last price - March, 2000.)

If I can be of any further help, please let me know.

Regards,

Colleen Marie Genadry, Administrative Assistant, Manpower
IBM Corporation
2070 State Route 52, Mail Stop E40, Dept. 13WA
Hopewell Jct., NY 12533-6531
T/L 532-2664
} From Outside call (845)-892-2664
FAX #: (845)-892-2003
E-Mail: cgenadry-at-us.ibm.com



From daemon Mon Jan 22 10:21:35 2001



From: ERIC :      biology-at-ucla.edu
Date: Mon, 22 Jan 2001 08:22:14 -0800
Subject: Cleaning Knife Boats?

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To my fellow Microscopists,

Here we use the LKB plastic knife boats for our glass knives on cutting 1
micron sections. To attach the knife boat to the glass I use clear nail
polish. The problem is how can I safely remove the nail polish and use the
knife boats again.

I have tried a low concentration of acetone which melts the boats. I have
tried a diluted nail polish remover which also melts the plastic boats and
deforms them.

Any suggestions?

Thanks,

Eric A. Rosen
UCLA Medical Center
Electron Microscopy Lab
Department of Pathology
Email: biology-at-ucla.edu or erosen-at-mednet.ucla.edu




From daemon Mon Jan 22 10:57:00 2001



From: J. A. Kiernan :      jkiernan-at-julian.uwo.ca
Date: Mon, 22 Jan 2001 11:53:50 -0500 (EST)
Subject: Re: Basal bodies

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On Thu, 18 Jan 2001, Karen Pawlowski wrote:

} I was wondering if there are any stains/methods that would allow the
} identification of basal bodies or centromeres in a non-dividing cell.
} I would like to determine the basal body position in a cell without
} resorting to serial TEM. My thinking is that I might be able to do
} it in whole cells with the right marker. Any comments?

This is classical cytology, so you need a classical method! Here
are a few suggestions.

1. Osmium tetroxide is a must, either in the primary fixative
or as a post-fix. A glutaraldehyde-osmium sequence, as for EM,
is excellent.

2. Plastic embedding followed by staining 1um sections with a
basic dye may be all you need. Otherwise, cut paraffin sections
as thinly as possible (The 19th century methods for centrosomes
were, according to McClung's Handbook, done this way, after
chrome-osmic fixatives.)

3. Try treating paraffin sections of postosmicated tissue with
ethyl gallate, which makes a more darkly coloured product at
sites of osmium binding. This method was used with great elegance
in a study of the hypothalamus and neurohypophysis by
D. G. Montemurro - J. Endocrinol. 35: 271-279 (1966).

4. Try Heidenhain's iron-haematoxylin method. With careful
differentiation this can make almost anything visible.

5. When you have found a good method let us all know about
it, by way of Histonet.

John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
E-mail: kiernan-at-uwo.ca
http://publish.uwo.ca/~jkiernan/index.htm




From daemon Mon Jan 22 11:52:59 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 22 Jan 2001 11:37:39 -0600
Subject: Re: Carbon in Steel Standards for WDX

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I didn't think that you could get a single-phase microstructure for carbon
in steel at a very high level of carbon. But I couldn't remember with
certainty, so I asked my friendly neighborhood materials scientist. Here is
his reply.
-------------
} At what levels can carbon be held in the matrix by solid solution? When
} does it force a second phase to appear?

About 0.02% C.

That's a huge range of carbon levels to expect homogenous
microstructure. Only thing I can think of would be to look for standards
with nickel to keep the steel austenitic, like an austenitic stainless
steel. Might be able to get quenched steel standards having a somewhat
uniform structure of martensite, though not purely homogenous.

So it begs the question, if the guy needs standards to analyze samples that
are homogenous, how are the samples being prepared?
-------------
I would be curious to know what sort of samples will be the subject of the
investigation. I suppose it might be possible to get some estimate by
probing a larger area, but the results would be subject to dis-similarities
in the microstructure and whatever errors result from dealing with a
two-phase structure.

C in iron is problematic, and I don't have a probe to get enough signal.
Besides, my friend gets good, cheap, fast results from a spectrographic lab
so that I haven't bothered to even try.

Warren S.

At 06:14 PM 1/19/2001 -0600, you wrote:
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From daemon Mon Jan 22 12:16:21 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Mon, 22 Jan 2001 10:13:07 -0800 (PST)
Subject: RE: Problems with blue staining

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Ok, here I go again :-)
We've checked the PBS pH and turned out to be just fine, about 7
(suposedly is 7.2). The glycerol was more acid though, down to 4.5. So
maybe it was the glycerol. But we performed a dot-blot test and it was
negative for that batch of glycerol: we fixed HRPeroxidase on
nitrocelulose paper and we tested 2 batches of DAB (an old and a new
one)to reveal it, and both performed equally up to the same
HRP-dilution. Then we added the supposedly bad glycerol:PBS and nothing
happened, the stain didn't fade, not even in 2 hours. So that is why
it's running us crazy. Maybe the DAB performs differently on semithin
tissue sections than over nitrocellulose paper.
The semithin sections, well they are 7 or 10 micrometers wide,in 53ª
paraffin, and a few months ago the immunostaining didn't work at all
(though the protocol has been working fine for 5 years), and then small
yellowish bubbles could be seen inside the tissue. And we never cleared
if they were residual paraffin or xylene -though we found out both
paraffin and xylene were doing wrong, since the paraffin had been at
56º for months -supposedly the polymers grew longer-, the xylene was
too polluted with paraffin and the AA was not pure at all. I'll never
forget again that EVERY single step on that protocol is killingly
important.
That is why I was asking if anyone had ever had any problems with
immunostainings ...
So, what do you think? Right now the protocol works fine some days, and
some others doesn't.

Albert Cardona
University of Barcelona

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From daemon Mon Jan 22 13:43:34 2001



From: Tony Owens :      towens-at-camscan-usa.com
Date: Mon, 22 Jan 2001 14:38:44 -0500
Subject: Re[2]: Question

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Just thought I would throw my two cents worth in regarding the
issue of fields. It seems that one of the problems is defining
exactly what we mean by an electric field. Faraday introduced the
concept of a field (or more precisely a "force field") simply as
model or construct used to describe the existence - i.e. the
observation - of electric forces. An electric field is
characterized or explored by "measuring" the magnitude and
direction of electric force experienced by a test charge (the
force vector). A collection of infinitely many force vectors
"defines" the electric field. At any point in the field, the
direction of the field is DEFINED as the direction of force on
the test charge, and the strength of the field is DEFINED as the
magnitude of the force experienced by the test charge. There are
different models for the mechanism of electric force - the
"original" Faraday-Maxwell theory, in which lines or tubes of
force represent a strain in the "ether" - to the more modern
theory of exchange of particles (photon-field theory). But the
important point here is that "field" is simply a concept used to
characterize the electric forces experienced by our imaginary
test charge (we'll say an electron, even though physicists use a
positive test charge by convention).

So maybe the question to ask is what electric force will an
imaginary, stationary electron experience in the "vicinity" of
the sample (either above the sample surface or within the
sample). Assuming that the sample is a perfect conductor, my
sense is that this stationary electron would not experience any
significant electric forces, indicating that (by definition) no
significant electric field exists.

However, real samples such as what we're discussing in this
thread will contain regions of varying electrical conductivity.
The best example is perhaps that of a charging dust particle,
around which a strong electrical "field" is generated - I believe
a stationary test charge will experience strong electrical forces
in this region. The reason the field is produced is the existence
of a buildup of charges on/in the dust particle, and the
close proximity of the surface of the sample which does not build
up this electric charge (perhaps semantics, but I would NOT say
the electrons ARE the field - they may be partly responsible for the
field, but the "field" itself is just a concept which
characterizes our observation that a test charge in this region
will experience a force which has magnitude and direction.

Therefore, it seems to me that any electrostatic discharge (ESD)
damage caused within a sample will be initiated by the generation
of such an electric field, which in turn can only be generated
when there are differences in electrical conductivity (otherwise,
how can a differential buildup of charges - which produces the
field - occur?)

Best regards,


Tony mailto:towens-at-camscan-usa.com

Tony Owens
CamScan USA Inc.
508 Thomson Park Dr.
Cranberry Twp., PA 16066
Tel: 724-772-7433
Fax: 724-772-7434
URL: www.camscan-usa.com

____________________________________________


Monday, January 22, 2001, 1:44:53 PM, You wrote:

ARS} ------------------------------------------------------------------------
ARS} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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ARS} Yes, please forgive me. My spelling is not very accurate when I am
ARS} responding so early in the morning. Your correction on Wehnelt is correct.
ARS} Please forgive any such errors in this response, as it is similar in
ARS} timing.

ARS} The electric field of interest is not between any parts of the column, but
ARS} rather between the electrons and the sample. I really don't want to try to
ARS} attempt any quantum exercises here, but the energy impressed on the
ARS} electrons results in their increased EM field effects. The acceleration of
ARS} the electrons controlled by the electron gun configuration controls the
ARS} energy afforded the electrons. What happens after the anode does not
ARS} affect the energy of the electrons in the beam.

ARS} The electrons are the field. The acceleration given to them by the gun
ARS} determine their acceleration, and thus their field and relativistic
ARS} effects. Their energy is determined by the fields in the gun structure
ARS} alone. Once they are given that energy, their travel through the column is
ARS} determined by the momentum they have been given. The fields they generate
ARS} as they travel through the sample surface are the direct result of the
ARS} energy they were provided with in the electron gun.

ARS} Here's a simple challenge - define the 'kinetic' energy of a fast moving
ARS} electron. As the kinetic energy is generally defined as the mass vs. the
ARS} velocity of that mass, you may have a problem. The best high energy
ARS} experiments have been unable to assign a 'mass' to the electron.
ARS} Apparently, the only mass associated with the electron is the Einsteinium
ARS} energy-mass equivalent of its charge. If there is any increase in an
ARS} electron's mass/energy by acceleration in an applied electric field, it is
ARS} to the electrons energy. Check with SLAC's experiments.

ARS} A fine point, but one with merit. It doesn't matter whether the distance
ARS} from anode to sample is 10 cm or 1000 cm, if the electron can travel the
ARS} distance without interference and external force. Thus the electro-magn
ARS} etic interaction of the electron with the sample is simply dependant on the
ARS} force originally impressed on the electron by the gun.

ARS} The field present at the sample surface will thus be determined by the
ARS} acceleration given the electrons by the gun and their current density. The
ARS} higher the beam current, the greater the field flux. The smaller the area
ARS} of the electron impingement on the surface, the higher the field flux. As
ARS} we work towards smaller circuit dimensions, these electron interactions
ARS} produce higher field flux densities as the circuit dimensions come closer
ARS} to the electron beam diameters.

ARS} You seem to want to separate the mass related kinetic effects from the
ARS} energy of the particle, but that can not be done. As the electron is
ARS} accelerated, its energy is apparently increased, rather than its mass. The
ARS} result is that the classical and relativistic effects normally related to
ARS} mass are instead seen in an increase in the energy, and thus, the field
ARS} effects of the electron with the sample.

ARS} On Friday, January 19, 2001 10:43 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
ARS} wrote:
} } { { File: ATT00000.txt; charset = windows-1252 } }

ARS} Thanks, Allen, for pointing out some of the issues here. By the way, It's
ARS} "Wehnelt", not "Wienault".

ARS} You are probably right about the ESD vs. EDS name. However, it was clear to
ARS} me what the original question was about.

ARS} Someone pointed out, that the cause of ESD (!) failure in electronic
ARS} devices
ARS} is, that the fields increase to beyond the breakdown strength of the
ARS} devices, which causes a high and sudden current to flow through the parts
ARS} where the fields are highest, which in turn heats and destroys the sample.
ARS} I
ARS} think, that is right. It's the fields that reach critical strength, which
ARS} then causes the current flow and destruction of the device. That's what I
ARS} meant when I said you get "zapped".

ARS} Now, about the electron microscope. Obviously I don't want to pronounce,
ARS} that electron microscopy is impossible. That would be stupid, wouldn't it?
ARS} However, as you said yourself, the sample is at ground potential, or very
ARS} near it, and so is the anode. In other words, there is not electric
ARS} potential (or very little) between the anode and the sample and thus no
ARS} electric field. Since the sample is grounded, as is the rest of the
ARS} microscope, there is no electric field between those, either. The energy of
ARS} an electron in an electric field is proportional to the electric field
ARS} (actually proportional to the square of the field, if my memory serves me).
ARS} No field - no electric energy. Of course that does not mean that the
ARS} electrons don't have energy. They have quite a lot of energy, on the order
ARS} of 20keV, by "falling" through the electric field between cathode and anode
ARS} and not being stopped by the anode. Since the sample is grounded (or should
ARS} be), the electric effects of the electron beam on the grounded sample will
ARS} be cancelled. What you have to contend with is the kinetic energy (20
ARS} keV/electron), which is transferred to the sample.

ARS} Now, I have always talked about grounded samples. That does not mean that
ARS} electric fields cannot be generated by the electron beam within the sample.
ARS} Obviously, you put electrons in the sample and they have to move out of the
ARS} sample. If there is any kind of resistance (an oxide layer, etc.), that
ARS} will
ARS} result in charging and an electric potential.

ARS} Michael Bode, Ph.D.
ARS} Soft Imaging System Corp.
ARS} 1675 Carr St., #105N
ARS} Lakewood, CO 80215
ARS} ===================================
ARS} phone: (888) FIND SIS
ARS} (303) 234-9270
ARS} fax: (303) 234-9271
ARS} email: mailto:info-at-soft-imaging.com
ARS} web: http://www.soft-imaging.com
ARS} ===================================



ARS} -----Original Message-----
} } From: Allen R. Sampson [mailto:ars-at-sem.com]
ARS} Sent: Friday, January 19, 2001 2:44 AM
ARS} To: 'Mike Bode'
ARS} Subject: RE: Question


ARS} Ok, apparently a simple physics lesson needed here.

ARS} First of all we are talking about ESD, or Electro-Static Discharge effects
ARS} here. Not EDS, or what in this field is known as Electron Dispersive
ARS} Spectroscopy.

ARS} Secondly, the electrons arriving at the sample surface in an SEM are
ARS} getting there with an acceleration that is roughly equivalent to the the
ARS} acceleration voltage impressed at the cathode. The sample is at, or very
ARS} near, ground potential. But the electrons in the beam are reaching the
ARS} sample at close to the accelerating voltage impressed. The grid or
ARS} "Wienault" of the electron gun is at a potential roughly +500V from the
ARS} accelerating voltage and is used to induce the electrons from the cathode
ARS} through the aperture in the anode, preventing the 'space charge' effects
ARS} around the cathode and providing the first electrostatic lens in the gun.

ARS} The potential of the anode of an electron microscope may be at ground
ARS} potential, but the electron beam is passing through the opening in the
ARS} anode. The anode is used as the second electrostatic lens, and has little
ARS} effect on the energy of the electrons passing through it. By its charge
ARS} opposite that of the electron beam, it encourages the first crossover of
ARS} the beam, but since the beam does not directly interact with it, there is
ARS} no direct energy exchange.

ARS} Precisely what kinetic energy can be transferred to the sample if there is
ARS} no energy differential between the electron potential at the anode and the
ARS} sample? Given your understanding of the processes involved, there can be
ARS} no discernable energy changes in the sample since the electrons have no
ARS} energy, and thus, there can be no discernable energy release from the
ARS} sample. In other words, in your view, electron microscopy is impossible.



ARS} On Thursday, January 18, 2001 2:24 PM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
ARS} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Yes, EDS is very real and chip manufacturers go to great length (end
} } expense) to avoid them. However, I think the mechanism is different:
} }
} } EDS means, that something collects static electricity (by walking on a
} } carpet, for example), then touches something that is at a very different
} } potential. That's what happens if you touch a metal after walking on that
} } carpet. The enormous potential difference create strong electric field
ARS} that
} } finally ionize the air and you get "zapped". The strong fields can
ARS} destroy
} } the electronics.
} }
} } In an SEM the sample is usually grounded and does not see strong fields.
} } True, the electrons are accelerated to 30 KV or more, but the sample does
} } not see that, as the anode usually sits at ground level with the Cathode
} } being at - 30 kV. What you need to be concerned about here is the kinetic
} } energy that is transferred to the sample and can heat it up considerable.
ARS} On
} } the other hand, if the sample is not grounded, there will be fields
} } developing. There are also other effects, such as residual organics
ARS} getting
} } "cracked" and forming a residue on the sample, but that's a different
ARS} story.
} }
} } Michael
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
ARS} Allen R. Sampson, Owner
ARS} Advanced Research Systems
ARS} 317 North 4th. Street
ARS} St. Charles, Illinois 60174
ARS} voice 630.513.7093 fax 630.513.7092


ARS} Allen R. Sampson, Owner
ARS} Advanced Research Systems
ARS} 317 North 4th. Street
ARS} St. Charles, Illinois 60174
ARS} voice 630.513.7093 fax 630.513.7092




From daemon Mon Jan 22 15:44:07 2001



From: Wright, John D. :      jwright-at-dugway-emh3.army.mil
Date: Mon, 22 Jan 2001 14:48:18 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe


From daemon Mon Jan 22 16:52:26 2001



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Mon, 22 Jan 2001 14:47:15 -0800
Subject: SEM: Imaging irradiated samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hiyo Listers,
What are the problems associated with imaging (SE and BSE) low level
radioactive samples (rocks)? They were irradiated for isotopic dating
~2 years ago and are now only a couple of times background? Does anyone
have experience with this low level radioactivity and SEM analysis? I
would appreciate any advice.
Many thanks,
Sarah

--
Sarah A.W. Lundberg
Electron Microanalysis and Imaging Laboratory
Department of Geoscience, UNLV
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010

EPMA Lab (702) 895-2660
SEM Lab (702) 895-2462
Office (702) 895-1134
Fax (702) 895-4064
Dept. Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMIL.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635




From daemon Mon Jan 22 17:50:46 2001



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Mon, 22 Jan 2001 15:47:30 -0800
Subject: Summer Internship in Transmission Electron Microscopy at Intel Co

Contents Retrieved from Microscopy Listserver Archives
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} We are pleased to announce the availability of a Summer Internship
} in Transmission Electron Microscopy at Intel Corporation, in Santa Clara,
} California. The period of employment will be summer, 2001, approximately
} from June through August, depending on availability. The goals of the
} internship will be to develop techniques using Electron Tomography to
} characterize the three-dimensional structure of elements of
} microelectronic devices. The ideal candidate should be a graduate student
} in materials science, physics, biophysics, or equivalent. In addition, the
} ideal candidate should be familiar with Transmission Electron Microscopy
} theory and practice, and should be very facile with computers. Prior
} experience with Electron Tomography techniques, FIB specimen preparation
} and Silicon Graphics would be especially beneficial. US citizenship, a
} Green Card, or an unrestricted work permit are required. For candidates
} geographically distant from California, relocation assistance may be
} available. Intel is an equal opportunity employer. Interested candidates
} are invited to contact John Mardinly by replying to this e-mail, or
} directly John.Mardinly-at-Intel.Com, 408-765-2346. Resumes must be submitted
} as text in an e-mail, not an attachment, or can be submitted through
} postal mail at: John Mardinly, Intel Corporation, Mail Stop SC2-24, 2200
} Mission College Blvd., Santa Clara, CA 95052-8119
}
}



From daemon Mon Jan 22 19:41:39 2001



From: Arthur Day :      ard-at-ansto.gov.au
Date: Tue, 23 Jan 2001 12:36:39 +1100
Subject: Re: SEM: Imaging irradiated samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hiyo Listers,
} What are the problems associated with imaging (SE and BSE) low level
} radioactive samples (rocks)? They were irradiated for isotopic dating
} ~2 years ago and are now only a couple of times background? Does anyone
} have experience with this low level radioactivity and SEM analysis? I
} would appreciate any advice.
} Many thanks,
} Sarah
}
} --
} Sarah A.W. Lundberg

Sarah,

From your description there should not be any problem with SE and BSE
imaging if the levels are only a few times "background".

If your samples have been irradiated (neutrons?) at some point in the
past then you might pick up some spontaneous signals from them in
your EDS detector, but that's probably about the extent of any
interference you'll get.

Cheers,
Arthur.





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Tue Jan 23 03:52:33 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Tue, 23 Jan 2001 03:37:17 -0600
Subject: RE: Re[2]: Question

Contents Retrieved from Microscopy Listserver Archives
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The concept of fields are not, I think, as arbitrary as you seem to
believe. Their introduction was not as an expedient to describe electric
forces, but rather DEFINED as their influence over distance, which could
not be explained by any other means. A fine point, perhaps, but one worth
making. Newton had given some explanation of the gravitational field, and
thus the underpinning of field theory. When it was discovered that
electricity and magnetism were related, it was obvious that the same
theories that explained the mass-gravity duality might be extended to
charge-magnetism . In each case, it was supposed that distant interactions
could be related to localized observations.

In our specific field, we are privy to the roots of quantum theory that
currently pervade our understanding of this world. The characteristic
x-ray spectrums that we rely on were a catalyst for the basic quantum
theories such as Pauli's, not to mention the photo-electric effects of PMTs
that were the foundation of Einstein's theory of Special Relativity. These
theories also support the concept of fields, although they couch them in
the exchange of discrete particles. This is used to explain the
empirically observed discrete, rather than continuous, exchanges of energy
between sub-atomic particles.

You digress to interactions of a sample with 'stationary' electrons. I
have written about electrons that have been given energy by acceleration in
electric fields. Apples and oranges. An electron that has been
accelerated by the typical electron beam instrument has far more energy
than a 'stationary' electron, and that energy is primarily available
through its interaction with matter. Can a 'stationary' electron produce
an inner shell electron vacancy? I can prove that an accelerated electron
can produce far greater yields than those produced by simple kinetic
theories. Producing an inner shell electron vacancy requires a defined
amount of energy and energy transfer at a distance that is simply not
available in a 'stationary' electron. As the electron has no measurable
dimension, the 'billiard ball' idea of kinetics can simply not explain such
an energy exchange or the spectral yield in a typical x-ray instrument.

A simple demonstration. Within your SEM or TEM is a circuit that provides
compensation for various accelerating voltages used. When you increase the
accelerating voltage, this circuit will increase the currents applied to
the condenser and objective lenses. Why? Because the electrons have more
energy at the increased accelerating voltages and require more force to be
applied to alter their trajectories to produce a similar demagnification
and focussing. The rest energy of these electrons has not changed, but
their total energy has. What repercussions does this have? Very simply,
their ability to interact with external fields and thus other charged
particles has increased while their susceptibility to those influences has
decreased.

In terms of fields, the accelerated electron produces an increased electric
field gradient in its immediate vicinity as its energy is increased. For a
single electron, this is a very localized effect. But in an electron beam
instrument, this effect can be spread over a much larger area as there is
considerable uncertainty in the location of individual electrons. In a
macro gradient produced by ESD, they are larger still. While the field
gradients are substantially different from those produced by electro-static
discharge, the effect and cause can be basically the same.

In ESD, two macro materials are brought in close contact that have greatly
differing electric potentials. Those electric differentials eventually
find a path through ionization of the interstitial gases to equalize the
electrical difference. The intervening gases provide a randomizing effect
that spreads the effect over a relatively large area. However, the energy
transfer behind the effect is the acceleration of electrons by the
differing electric potentials of the materials. Those accelerated
electrons hit the secondary material with the same enhanced energy as those
accelerated by artificial means.

Whether you use particle energy and fields or wave functions, the math is
similar. The electron has a rest mass, that is indistinguishable from its
charge. When accelerated through electric fields, the electron gains
energy that is then available externally, through interactions with the
fields it generates, or the extension of its wave function. In either
case, as the energy of the electron is increased, the gradient of its field
or wave function increases. That creates an increasing EM field that on a
small scale can create large electric and magnetic fields gradients that
can emulate larger scale effects within reason.

On Monday, January 22, 2001 1:39 PM, Tony Owens
[SMTP:towens-at-camscan-usa.com] wrote:
} Just thought I would throw my two cents worth in regarding the
} issue of fields. It seems that one of the problems is defining
} exactly what we mean by an electric field. Faraday introduced the
} concept of a field (or more precisely a "force field") simply as
} model or construct used to describe the existence - i.e. the
} observation - of electric forces. An electric field is
} characterized or explored by "measuring" the magnitude and
} direction of electric force experienced by a test charge (the
} force vector). A collection of infinitely many force vectors
} "defines" the electric field. At any point in the field, the
} direction of the field is DEFINED as the direction of force on
} the test charge, and the strength of the field is DEFINED as the
} magnitude of the force experienced by the test charge. There are
} different models for the mechanism of electric force - the
} "original" Faraday-Maxwell theory, in which lines or tubes of
} force represent a strain in the "ether" - to the more modern
} theory of exchange of particles (photon-field theory). But the
} important point here is that "field" is simply a concept used to
} characterize the electric forces experienced by our imaginary
} test charge (we'll say an electron, even though physicists use a
} positive test charge by convention).
}
} So maybe the question to ask is what electric force will an
} imaginary, stationary electron experience in the "vicinity" of
} the sample (either above the sample surface or within the
} sample). Assuming that the sample is a perfect conductor, my
} sense is that this stationary electron would not experience any
} significant electric forces, indicating that (by definition) no
} significant electric field exists.
}
} However, real samples such as what we're discussing in this
} thread will contain regions of varying electrical conductivity.
} The best example is perhaps that of a charging dust particle,
} around which a strong electrical "field" is generated - I believe
} a stationary test charge will experience strong electrical forces
} in this region. The reason the field is produced is the existence
} of a buildup of charges on/in the dust particle, and the
} close proximity of the surface of the sample which does not build
} up this electric charge (perhaps semantics, but I would NOT say
} the electrons ARE the field - they may be partly responsible for the
} field, but the "field" itself is just a concept which
} characterizes our observation that a test charge in this region
} will experience a force which has magnitude and direction.
}
} Therefore, it seems to me that any electrostatic discharge (ESD)
} damage caused within a sample will be initiated by the generation
} of such an electric field, which in turn can only be generated
} when there are differences in electrical conductivity (otherwise,
} how can a differential buildup of charges - which produces the
} field - occur?)
}
} Best regards,
}
}
} Tony mailto:towens-at-camscan-usa.com
}
}
} Monday, January 22, 2001, 1:44:53 PM, You wrote:
}
} ARS} Yes, please forgive me. My spelling is not very accurate when I am
} ARS} responding so early in the morning. Your correction on Wehnelt is
correct.
} ARS} Please forgive any such errors in this response, as it is similar
in
} ARS} timing.
}
} ARS} The electric field of interest is not between any parts of the
column, but
} ARS} rather between the electrons and the sample. I really don't want to
try to
} ARS} attempt any quantum exercises here, but the energy impressed on the
} ARS} electrons results in their increased EM field effects. The
acceleration of
} ARS} the electrons controlled by the electron gun configuration controls
the
} ARS} energy afforded the electrons. What happens after the anode does
not
} ARS} affect the energy of the electrons in the beam.
}
} ARS} The electrons are the field. The acceleration given to them by the
gun
} ARS} determine their acceleration, and thus their field and relativistic
} ARS} effects. Their energy is determined by the fields in the gun
structure
} ARS} alone. Once they are given that energy, their travel through the
column is
} ARS} determined by the momentum they have been given. The fields they
generate
} ARS} as they travel through the sample surface are the direct result of
the
} ARS} energy they were provided with in the electron gun.
}
} ARS} Here's a simple challenge - define the 'kinetic' energy of a fast
moving
} ARS} electron. As the kinetic energy is generally defined as the mass
vs. the
} ARS} velocity of that mass, you may have a problem. The best high energy
} ARS} experiments have been unable to assign a 'mass' to the electron.
} ARS} Apparently, the only mass associated with the electron is the
Einsteinium
} ARS} energy-mass equivalent of its charge. If there is any increase in
an
} ARS} electron's mass/energy by acceleration in an applied electric
field, it is
} ARS} to the electrons energy. Check with SLAC's experiments.
}
} ARS} A fine point, but one with merit. It doesn't matter whether the
distance
} ARS} from anode to sample is 10 cm or 1000 cm, if the electron can travel
the
} ARS} distance without interference and external force. Thus the
electro-magn
} ARS} etic interaction of the electron with the sample is simply dependant
on the
} ARS} force originally impressed on the electron by the gun.
}
} ARS} The field present at the sample surface will thus be determined by
the
} ARS} acceleration given the electrons by the gun and their current
density. The
} ARS} higher the beam current, the greater the field flux. The smaller
the area
} ARS} of the electron impingement on the surface, the higher the field
flux. As
} ARS} we work towards smaller circuit dimensions, these electron
interactions
} ARS} produce higher field flux densities as the circuit dimensions come
closer
} ARS} to the electron beam diameters.
}
} ARS} You seem to want to separate the mass related kinetic effects from
the
} ARS} energy of the particle, but that can not be done. As the electron
is
} ARS} accelerated, its energy is apparently increased, rather than its
mass. The
} ARS} result is that the classical and relativistic effects normally
related to
} ARS} mass are instead seen in an increase in the energy, and thus, the
field
} ARS} effects of the electron with the sample.
}
} ARS} On Friday, January 19, 2001 10:43 AM, Mike Bode
[SMTP:mb-at-Soft-Imaging.com]
} ARS} wrote:
} } } { { File: ATT00000.txt; charset = windows-1252 } }
}
} ARS} Thanks, Allen, for pointing out some of the issues here. By the way,
It's
} ARS} "Wehnelt", not "Wienault".
}
} ARS} You are probably right about the ESD vs. EDS name. However, it was
clear to
} ARS} me what the original question was about.
}
} ARS} Someone pointed out, that the cause of ESD (!) failure in electronic
} ARS} devices
} ARS} is, that the fields increase to beyond the breakdown strength of the
} ARS} devices, which causes a high and sudden current to flow through the
parts
} ARS} where the fields are highest, which in turn heats and destroys the
sample.
} ARS} I
} ARS} think, that is right. It's the fields that reach critical strength,
which
} ARS} then causes the current flow and destruction of the device. That's
what I
} ARS} meant when I said you get "zapped".
}
} ARS} Now, about the electron microscope. Obviously I don't want to
pronounce,
} ARS} that electron microscopy is impossible. That would be stupid,
wouldn't it?
} ARS} However, as you said yourself, the sample is at ground potential, or
very
} ARS} near it, and so is the anode. In other words, there is not electric
} ARS} potential (or very little) between the anode and the sample and thus
no
} ARS} electric field. Since the sample is grounded, as is the rest of the
} ARS} microscope, there is no electric field between those, either. The
energy of
} ARS} an electron in an electric field is proportional to the electric
field
} ARS} (actually proportional to the square of the field, if my memory
serves me).
} ARS} No field - no electric energy. Of course that does not mean that the
} ARS} electrons don't have energy. They have quite a lot of energy, on the
order
} ARS} of 20keV, by "falling" through the electric field between cathode
and anode
} ARS} and not being stopped by the anode. Since the sample is grounded (or
should
} ARS} be), the electric effects of the electron beam on the grounded
sample will
} ARS} be cancelled. What you have to contend with is the kinetic energy
(20
} ARS} keV/electron), which is transferred to the sample.
}
} ARS} Now, I have always talked about grounded samples. That does not mean
that
} ARS} electric fields cannot be generated by the electron beam within the
sample.
} ARS} Obviously, you put electrons in the sample and they have to move out
of the
} ARS} sample. If there is any kind of resistance (an oxide layer, etc.),
that
} ARS} will
} ARS} result in charging and an electric potential.
}
}
} ARS} -----Original Message-----
} } } From: Allen R. Sampson [mailto:ars-at-sem.com]
} ARS} Sent: Friday, January 19, 2001 2:44 AM
} ARS} To: 'Mike Bode'
} ARS} Subject: RE: Question
}
}
} ARS} Ok, apparently a simple physics lesson needed here.
}
} ARS} First of all we are talking about ESD, or Electro-Static Discharge
effects
} ARS} here. Not EDS, or what in this field is known as Electron
Dispersive
} ARS} Spectroscopy.
}
} ARS} Secondly, the electrons arriving at the sample surface in an SEM are
} ARS} getting there with an acceleration that is roughly equivalent to the
the
} ARS} acceleration voltage impressed at the cathode. The sample is at, or
very
} ARS} near, ground potential. But the electrons in the beam are reaching
the
} ARS} sample at close to the accelerating voltage impressed. The grid or
} ARS} "Wienault" of the electron gun is at a potential roughly +500V from
the
} ARS} accelerating voltage and is used to induce the electrons from the
cathode
} ARS} through the aperture in the anode, preventing the 'space charge'
effects
} ARS} around the cathode and providing the first electrostatic lens in the
gun.
}
} ARS} The potential of the anode of an electron microscope may be at
ground
} ARS} potential, but the electron beam is passing through the opening in
the
} ARS} anode. The anode is used as the second electrostatic lens, and has
little
} ARS} effect on the energy of the electrons passing through it. By its
charge
} ARS} opposite that of the electron beam, it encourages the first
crossover of
} ARS} the beam, but since the beam does not directly interact with it,
there is
} ARS} no direct energy exchange.
}
} ARS} Precisely what kinetic energy can be transferred to the sample if
there is
} ARS} no energy differential between the electron potential at the anode
and the
} ARS} sample? Given your understanding of the processes involved, there
can be
} ARS} no discernable energy changes in the sample since the electrons have
no
} ARS} energy, and thus, there can be no discernable energy release from
the
} ARS} sample. In other words, in your view, electron microscopy is
impossible.
}
}
}
} ARS} On Thursday, January 18, 2001 2:24 PM, Mike Bode
[SMTP:mb-at-Soft-Imaging.com]
} ARS} wrote:
} } }
} } }
} } } Yes, EDS is very real and chip manufacturers go to great length (end
} } } expense) to avoid them. However, I think the mechanism is different:
} } }
} } } EDS means, that something collects static electricity (by walking on a
} } } carpet, for example), then touches something that is at a very
different
} } } potential. That's what happens if you touch a metal after walking on
that
} } } carpet. The enormous potential difference create strong electric field
} ARS} that
} } } finally ionize the air and you get "zapped". The strong fields can
} ARS} destroy
} } } the electronics.
} } }
} } } In an SEM the sample is usually grounded and does not see strong
fields.
} } } True, the electrons are accelerated to 30 KV or more, but the sample
does
} } } not see that, as the anode usually sits at ground level with the
Cathode
} } } being at - 30 kV. What you need to be concerned about here is the
kinetic
} } } energy that is transferred to the sample and can heat it up
considerable.
} ARS} On
} } } the other hand, if the sample is not grounded, there will be fields
} } } developing. There are also other effects, such as residual organics
} ARS} getting
} } } "cracked" and forming a residue on the sample, but that's a different
} ARS} story.
} } }
} } } Michael
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================

Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Tue Jan 23 03:56:04 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 23 Jan 2001 09:53:11 +0000 (GMT Standard Time)
Subject: Re: Cleaning Knife Boats?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You could attach the boats with wax. They can then be
reused.

Dave


On Mon, 22 Jan 2001 08:22:14 -0800 ERIC {biology-at-ucla.edu}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To my fellow Microscopists,
}
} Here we use the LKB plastic knife boats for our glass knives on cutting 1
} micron sections. To attach the knife boat to the glass I use clear nail
} polish. The problem is how can I safely remove the nail polish and use the
} knife boats again.
}
} I have tried a low concentration of acetone which melts the boats. I have
} tried a diluted nail polish remover which also melts the plastic boats and
} deforms them.
}
} Any suggestions?
}
} Thanks,
}
} Eric A. Rosen
} UCLA Medical Center
} Electron Microscopy Lab
} Department of Pathology
} Email: biology-at-ucla.edu or erosen-at-mednet.ucla.edu
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Jan 23 04:24:25 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Tue, 23 Jan 2001 10:21:30 +0000 (GMT)
Subject: Alcohol, Xylene and Paraffin

Contents Retrieved from Microscopy Listserver Archives
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Albert,

Solvents and polymers are my business, rather than clearing sections (I last
did that sort of thing many years ago, in my schooldays), but here's some
chemical background. But those with more direct experience in this field
might know better.

On Mon, 22 Jan 2001, Grup Epigenetica wrote:

} Hello everybody
} My questions are:

} 1- Is it very important to use an extremly pure ethanol (AA) to remove
} the xylene from the semithin sections placed on slides?

I doubt it very much. DRY is important, for if water is present one might
end up with xylene-water emulsion. But I seem to remember that denatured
ethanol (i.e. with some methanol but not all the other things which are put
into ordinary "methylated spirit) was OK.

} 2- Can the tissue burst and the cells disrupt if it is not 100% pure?

If you are finding trouble with ethanol, I would think that isopropanol
(propan-2-ol) MIGHT be even better, since it has greater molar volume. But
because of its greater molar volume it would work more slowly. Paint
strippers, which work by swelling and bursting, rely on SMALL molar volume
for their effect. Small traces of water AND of xylene tend to evaporate
out of propan-2-ol, since in this environment their partial vapour
pressure is greater with an azeotropic effect.

} 3- Can the xylene stay within the tissue if the AA is not 100% pure,
} as little yellow bubbles?

Very unlikely, unless there is water there.

} 4- What happens if the paraffin has been stored at 56 degrees for too
} long? Can it turn into longer polymers and then be harder or impossible
} to remove from the sections? How may I detect so in the sections, is it
} visible?

The oxidation products of paraffins are unlikely to be polymeric.
Generally oxidation reduces the molecular weight of polythene, which after
all is a super-long paraffin. Yellow sticky polymeric nasties only form
from unsaturated oils such as linseed oil and overheated cooking oil: they
can cross-link because of their double bonds. But if the paraffin is not
properly washed out by the xylene it could be re-precipitated by the
alcohol, whether clean or degraded.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Tue Jan 23 04:30:44 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Tue, 23 Jan 2001 22:51:38 +1000
Subject: RE: Cleaning Knife Boats?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is unlikely that there is a solvent that will dissolve nail polish
without also attacking the boats, since the solvents in nail polish
dissolve the boats. Your only option here is to use another "glue".
Good old dental wax is probably the simplest.

Chris

Date sent: Mon, 22 Jan 2001 08:22:14 -0800
To: Microscopy-at-sparc5.microscopy.com
} From: ERIC {biology-at-ucla.edu}


Agreed. Wax is the way to fasten boats. However, I much prefer dental wax over
common paraffin wax which is more prone to cause leaky boats.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, January 23, 2001 7:53 PM, Patton, David
[SMTP:David.Patton-at-uwe.ac.uk] wrote:
}
}
} You could attach the boats with wax. They can then be
} reused.
}
} Dave
}
}
} On Mon, 22 Jan 2001 08:22:14 -0800 ERIC {biology-at-ucla.edu}
} wrote:
}
}
} }
} } To my fellow Microscopists,
} }
} } Here we use the LKB plastic knife boats for our glass knives on cutting 1
} } micron sections. To attach the knife boat to the glass I use clear nail
} } polish. The problem is how can I safely remove the nail polish and use the
} } knife boats again.
} }
} } I have tried a low concentration of acetone which melts the boats. I have
} } tried a diluted nail polish remover which also melts the plastic boats and
} } deforms them.
} }
} } Any suggestions?
} }
} } Thanks,
} }
} } Eric A. Rosen
} } UCLA Medical Center
} } Electron Microscopy Lab
} } Department of Pathology
} } Email: biology-at-ucla.edu or erosen-at-mednet.ucla.edu
} }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}



From daemon Tue Jan 23 07:08:22 2001



From: Lena Falk :      lklfalk-at-fy.chalmers.se
Date: Tue, 23 Jan 2001 13:57:51 +0100
Subject: TEM post-doc position at Chalmers, Sweden

Contents Retrieved from Microscopy Listserver Archives
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POST-DOCTORAL POSITION
"Transmission Electron Microscopy of Carbon Nanotubes"

A post-doctoral position in the field of carbon nanotube science is
available in the Division for Microscopy and Microanalysis at Chalmers
University of Technology, Gothenburg, Sweden. Qualified candidates
should have a Ph.D. in physics, materials science or chemistry, and a
documented background in transmission electron microscopy. It will be
considered an advantage if the candidate has some experimental
experience of electron energy loss spectroscopy (EELS) and / or high
resolution lattice imaging. The position is immediately available, and
the appointment may last up to 24 months.

The position is part of a consortium of seven research groups at
Universities in Gothenburg and Uppsala in Sweden. The consortium, which
is funded by the Swedish Foundation for Strategic Research (SSF), will
focus on the experimental and theoretical investigation of structure and
electronic properties of carbon nanotubes with the ultimate goal of
implementation of prototype electronic devices based on carbon
nanotubes. An interdisciplinary approach, provided by the diversity of
the research groups in the consortium and strong interaction between the
groups is intended to facilitate this aim.

The microscopy work will be carried out on a Philips CM200 Supertwin
transmission electron microscope (TEM) with a field emission gun (FEG)
and surrounding interactive instrumentation. The FEG TEM is equipped
with the Gatan imaging filter (GIF), which produces energy filtered
electron images and diffraction patterns as well as electron energy loss
spectra, a Gatan off-axis CCD camera and a Link Isis energy dispersive
X-ray (EDX) system. There are also other electron microscopes in the
laboratory, e.g. a Jeol 2000-FX TEM/STEM/SEM and a CamScan SEM.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with postal
and e-mail addresses to:

Associate Professor Lena Falk, Department of Experimental Physics,
Chalmers University of Technology, SE-412 96 Göteborg, Sweden
e-mail: lklfalk-at-fy.chalmers.se; fax: +46 31 772 3224; phone: +46 31
772 3321

Information about the Division for Microscopy and Microanalysis can be
found at
http://fy.chalmers.se/microscopy


____________________________

Associate Professor Lena Falk
Department of Experimental Physics,
Chalmers University of Technology,
SE-412 96 Göteborg, SWEDEN

tel: +46 31 772 3321
fax: +46 31 772 3224
e-mail: lklfalk-at-fy.chalmers.se


From daemon Tue Jan 23 07:17:04 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 23 Jan 2001 13:14:08 +0000 (GMT Standard Time)
Subject: Re: RE: Cleaning Knife Boats?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Jim. I should have said it was dental wax. You can
get it from an EM supply company. We have a little LKB
multiplate which allows you to, warm the knife, melt the
dental wax and apply it with a sort of warmed metal spatula.

Dave


On Tue, 23 Jan 2001 22:51:38 +1000 Jim at ProSciTech
{jim-at-proscitech.com} wrote:

} Agreed. Wax is the way to fasten boats. However, I much prefer dental wax over
} common paraffin wax which is more prone to cause leaky boats.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Tuesday, January 23, 2001 7:53 PM, Patton, David
} [SMTP:David.Patton-at-uwe.ac.uk] wrote:
} }
} }
} } You could attach the boats with wax. They can then be
} } reused.
} }
} } Dave
} }
} }
} } On Mon, 22 Jan 2001 08:22:14 -0800 ERIC {biology-at-ucla.edu}
} } wrote:
} }
} }
} } }
} } } To my fellow Microscopists,
} } }
} } } Here we use the LKB plastic knife boats for our glass knives on cutting 1
} } } micron sections. To attach the knife boat to the glass I use clear nail
} } } polish. The problem is how can I safely remove the nail polish and use the
} } } knife boats again.
} } }
} } } I have tried a low concentration of acetone which melts the boats. I have
} } } tried a diluted nail polish remover which also melts the plastic boats and
} } } deforms them.
} } }
} } } Any suggestions?
} } }
} } } Thanks,
} } }
} } } Eric A. Rosen
} } } UCLA Medical Center
} } } Electron Microscopy Lab
} } } Department of Pathology
} } } Email: biology-at-ucla.edu or erosen-at-mednet.ucla.edu
} } }
} } }
} } }
} }
} } ----------------------------------------
} } Patton, David
} } Email: David.Patton-at-uwe.ac.uk
} } "University of the West of England"
} }
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Jan 23 07:36:23 2001



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Tue, 23 Jan 2001 07:36:51 -0600
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe


From daemon Tue Jan 23 07:53:51 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Tue, 23 Jan 2001 07:46:27 -0600
Subject: RE: Imaging irradiated samples

Contents Retrieved from Microscopy Listserver Archives
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Sarah,

At the rad levels you describe, the effects will be difficult to notice. I
have examined specimens ranging up to 10s of R at a foot. Not many that hot
lately, thankfully. Emissions from my specimens have included alpha, beta,
and gamma. Gamma was my least favorite. Highly active alpha/beta would
significantly impair imaging and EDS by adding noise to the system. The
signal to noise of the SE/BSE system was degraded and EDS peaks broadened.
Have you ever seen 80% dead time *before* you turn on the beam? On one
occasion, the EDS was totally locked out at 100% DT. Dead time...Hummmm...
OTOH those high energy gammas would escape the chamber and take aim at me.
I was hiding behind two - four inches of temporary lead wall in front of the
chamber.

Woody

}
} -------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hiyo Listers,
} What are the problems associated with imaging (SE and BSE) low level
} radioactive samples (rocks)? They were irradiated for isotopic dating
} ~2 years ago and are now only a couple of times background?
} Does anyone
} have experience with this low level radioactivity and SEM analysis? I
} would appreciate any advice.
} Many thanks,
} Sarah
}
} --
} Sarah A.W. Lundberg
} Electron Microanalysis and Imaging Laboratory
} Department of Geoscience, UNLV
} 4505 S. Maryland Parkway Box 454010
} Las Vegas, NV 89154-4010
}
} EPMA Lab (702) 895-2660
} SEM Lab (702) 895-2462
} Office (702) 895-1134
} Fax (702) 895-4064
} Dept. Office (702) 895-3262
}
} lundberg-at-nevada.edu
} http://www.unlv.edu/Colleges/Sciences/Geoscience/EMIL.htm
}
} Home:
} 8025 W. Russell Rd. Apt. #1117
} Las Vegas, NV 89113
} (702) 871-9635
}
}
}


From daemon Tue Jan 23 08:16:50 2001



From: klaus neumann :      bikneu-at-krzsun.med-rz.uni-saarland.de
Date: Tue, 23 Jan 2001 15:15:43 +0100
Subject: RE: cleaning plastic knife boats

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,
sorry but I have already deleted the original question, but I suggest
"physical" cleaning by liquid nitrogen. This should also remove the nail
polish.

Hope this helps

Klaus Neumann
Dr. Klaus Neumann
2.5 Med. Biologie
Universität des Saarlandes
D-66421 Homburg
Tel: ++49-6841-16-62 55
Fax: ++49-6841-16-62 56
http://www.med-rz.uni-sb.de/med_fak/biologie



From daemon Tue Jan 23 17:35:17 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Tue, 23 Jan 2001 15:19:13 -0800 (PST)
Subject: Re: LM - problems with paraffin, xylene and AA

Contents Retrieved from Microscopy Listserver Archives
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Thank you Carmen Martin
Yes, we do also use our dehydratation series a lot, up to 60 slides
-which roughly means 2 weeks- and for sure the clearest sign is they
don't get stained. But it has it all puzzling us since anything seemed
to be wrong but everything failed -or so it looks.

Albert Cardona
University of Barcelona. Spain.


__________________________________________________
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From daemon Wed Jan 24 01:49:20 2001



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Date: Tue, 23 Jan 2001 17:15:21 -0800
Subject: Detective Software to Find Anything

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From daemon Wed Jan 24 04:13:09 2001



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Wed, 24 Jan 2001 09:54:46 -0000
Subject: Histoknifemaker

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I have been filtered out of posting responses by a quirk of my e-mail
address so this info is a little out of date but unavoidably so.

For those people with TAAB or the branded Reichert-Jung Histoknifemakers we
are still able to supply many of the spare parts for instruments up to 20
years old, but don't all rush at once!

This also confirms that we are still here live and well in Blighty,

Regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England

Tel ++44 118 981 7775
Fax ++44 118 981 7881


From daemon Wed Jan 24 05:57:34 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Wed, 24 Jan 2001 12:51:47 +0100
Subject: Antwort: Cleaning Knife Boats?

Contents Retrieved from Microscopy Listserver Archives
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Hi list,

may I just add to the discussion about the knife boats:

yes, if you use dental wax to glue the Truf to the knife (with the Leica
Multiplate, especially designed for that purpose its really easy) the boat
can be reused a few times.

HOWEVER:
1. breaking off the Truf from the knife and scratching away the old
hardened wax may damage the Truf - leaking may result from this! Can be
frustrating if you have perfect sections of a precious sample and see them
dive away ...
2. Esp. with ultrathins (but also with semithins) you must be aware of
possible cross contamination of old sections to the new set (I have no idea
if there is a GLP rule on that but in a hospital mixing up different
samples might cause serious problems)!

BTW the "LKB"Trufs are "Leica"Trufs now, but call them as you like it :-)

Best regards,

Joachim

Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350




From daemon Wed Jan 24 08:42:43 2001



From: Ryan Mitchell :      gtma-rdm-at-texas.net
Date: Wed, 24 Jan 2001 08:42:59 -0800
Subject: Unsubscribe

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From daemon Wed Jan 24 08:47:58 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 24 Jan 2001 09:45:08 -0500
Subject: TEM calibration

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi all,
I just wanted to run some interesting calibration data by you. We are working with some small particles and have to have reasonable size calibrations at both 80 and 100kV on a Philips EM-400 microscope (~20 years old). Calibration using replica gratings and catalase lattice spacing give us values somewhat below the magnifications indicated by the mag. readout on the panel. This is fine. The important thing is that we know what the true mags are.
One thing we did find is that the magnifications are consistantly slightly lower in value at 100kV than at 80kV. This surprised me at first but I am assuming that the difference is due to the higher acceleration of the electrons at 100kV. Am I correct to assume that higher energy electrons would be influenced less by the lenses, resulting in lower x-over point, which could result in a slight decrease in the magnification?
I suspect that newer microscopes could compensate for this through the software programming and will be doing the calibration at different kV values on our CM-10 to verify this.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



From daemon Wed Jan 24 09:22:17 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Wed, 24 Jan 2001 10:58:10 -0500
Subject: Printers - opinions, please

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Allen,

I am not sure I am following you. You say

"The best high energy experiments have been unable to assign a 'mass' to the
electron."

To my knowledge and from a list of "fundamental constants" in my "Solid
State Physics" book by Ashcroft and Mermin, the rest mass of an electron is
9.1095 x 10^(-31) kg. Just multiply that wit 1/2 v*v and you have the
kinetic Energy of the electron. Of course you have to take into account
relativistic effects. Or just multiply it with v and you have the momentum.

I think, you mistake the mass of the electron with that of the neutrino,
which indeed they have not been able to determine, although there are
indications from recent high energy physics measurements. But that's
irrelevant here.



Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Allen R. Sampson [mailto:ars-at-sem.com]
Sent: Monday, January 22, 2001 4:21 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


Hi, all,

I'm back to ask for more help. Thanks in advance, before I forget to My colleague is gathering some information on Tektronix Phaser 950 color printers. Specifically she would like to know:

- the quality of b/w prints (are they publication quality?) Some color printers make wonderful color prints and awful b/w prints.

- what sort of paper we would need for the best b/w prints.

Any comments, pros and cons from users, or people who have tried this product would be very welcome.

Please contact me offline and I'll forward the replies to her.

Thanks again.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Wed Jan 24 10:06:30 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 24 Jan 2001 11:42:56 -0500
Subject: Re: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Debby,

Yes, the focussing action of the lens, as given by d2z/dr2, is proportional
to (field) squared over voltage. [Check, e.g. Joy, Romig & Goldstein,
Principles of Analytical Electron Microscopy, pages 44-45.] I'l be
interested to hear whether newer TEMs do compensate for this or not.

Gill
----- Original Message -----
} From: "Debby Sherman" {dsherman-at-purdue.edu}
To: "message to: MSA list" {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 24, 2001 7:45 AM







Hi all,
I just wanted to run some interesting calibration data by you. We are
working with some small particles and have to have reasonable size calibrations
at both 80 and 100kV on a Philips EM-400 microscope (~20 years old).
Calibration using replica gratings and catalase lattice spacing give us values
somewhat below the magnifications indicated by the mag. readout on the panel.
This is fine. The important thing is that we know what the true mags are.
One thing we did find is that the magnifications are consistantly slightly
lower in value at 100kV than at 80kV. This surprised me at first but I am
assuming that the difference is due to the higher acceleration of the electrons
at 100kV. Am I correct to assume that higher energy electrons would be
influenced less by the lenses, resulting in lower x-over point, which could
result in a slight decrease in the magnification?
I suspect that newer microscopes could compensate for this through the
software programming and will be doing the calibration at different kV values on
our CM-10 to verify this.
Debby


Dear Debby,

It is true that the lens currents will be higher at 100 kV than at 80 kV;
however, even a 20-year-old microscope takes this into account and sets
the lens currents to match the accelerating voltage. It seems that in
your case this correction is slightly off resulting in the systematically
lower mags. The newer microscopes will also set the lens currents to
match the voltage, but whether this is done through software or
electromechanically I don't know.

Yours,


Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Wed Jan 24 10:56:56 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 24 Jan 2001 11:55:49 -0500
Subject: 2 Month Leave

Contents Retrieved from Microscopy Listserver Archives
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To all:

I will be out of the lab for two months. Please do not include me in
messages to the ListServer for the period 1/26/01-4/1/01.

Thanks!
Peggy Sherwood
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed Jan 24 11:37:32 2001



From: Nancy Robertson :      pfnlr-at-UAA.ALASKA.EDU
Date: Wed, 24 Jan 2001 08:37:22 -0900
Subject: Microtome room

Contents Retrieved from Microscopy Listserver Archives
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To all:

I have a small inner room within my office that is 6.2 5X 3.8 ft. with a
high ceiling. The building was built about 1950 with some of the same
electrical features. I would like to purchase a microtome (basic for
cutting thin sections of plant tissue for TEM) for the little room, and
the electrician asked if there were special requirements before the
purchase is made. Could someone help me?

Thanks,

Nancy Robertson
Research Plant Pathologist
USDA/ARS
533 E. Fireweed Ave.
Palmer AK 99645


From daemon Wed Jan 24 13:27:20 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Wed, 24 Jan 2001 14:23:54 -0500
Subject: EFTEM of 2 nm features

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I have been trying for a while to obtain EFTEM images on a series of polymers, but they wouldn't
cooperate. Our group is investigating ionomers (i.e polymers with a hydrophobic backbone and approx.
5 mol % of either sulfonic acid or carboxylic acid groups that are neutralized with different metal
ions). We know from STEM that these metal ion neutralized acid groups form aggregates in the 2 nm
range within a hydrophobic matrix and we would like to know the elemental distribution of metal ions
and O (and S in the case of SO3H). I use a JEOL210F FEG with GIF at 200kV. Has anybody ever tried to
resolve such small structures by EFTEM ? I'd appreciate tips and tricks as well as some useful
references.

Thanks for your help,

Andreas

*************************************************
Dr. Andreas Taubert
Materials Science and Engineering Dept.
3231 Walnut Street
The University of Pennsylvania=20
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************




From daemon Wed Jan 24 14:00:17 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 24 Jan 2001 15:51:14 -0400
Subject: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

I've just run into a problem (and, I think, partially solved it) that
may affect a lot of beam instruments with water-cooled diffusion pumps.
We'd been having some problems lately with at least one of the diff
pumps on our ESEM shutting itself off because it thought it wasn't being
cooled enough. I checked our little Neslab chiller unit; it seemed OK, and
since I'd just replaced the pump in it last year, I figured that there
really couldn't be a problem with that....
So I was starting to worry that maybe we had a diff pump or at least a
sensor starting to fail, but then this morning, the other diff pump also
shut itself down. Hello, thinks I, then this has to be a cooling problem. So
I disconnect the outflow from the chiller, and check the flow. Damn, lots of
water coming out....must be something else. But then I get a bright
idea....let's check the inline filter. Turns out, the filter is getting a
bit full of crud. Green crud. Ok, so I put a new one in. Then I get a second
bright idea (my personal daily limit). Let's check the water pressure/flow
returning from the ESEM. ....Oh oh, very low. So I disconnect both inlet and
outlet lines from the ESEM, stick the nozzle of a can of compressed air in
one, and let fly. Wish I hadn't had the other line aimed at myself just
then, but that's beside the point. About a half a litre of pretty dirty
water came shooting out, including a surprising number of little solid bits
of stuff. Judging by their blue-green colour, they're copper oxides,
presumably from the water lines in my slowly-decomposing ESEM.
Anyway, after that system flush, the flow seems a lot better, and now
the diff pumps seem to be staying on.
I don't believe it says anywhere in the manual that one should regularly
do this kind of procedure on one's beam instrument, but I wonder if this is
a common problem. Maybe some of you do a flush like this on a regular
(annual?) basis?
The water in our system is RO, and I don't put anything in to treat for
algae, since that doesn't seem to be a problem. Yet there's obviously some
corrosion going on. Is there a way to buffer the water to ensure a neutral
pH that will certainly not bother the instrument? I'm assuming our water is
a bit on the acid side, for this corrosion to be happening (?).


F. C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Dartmouth, Nova Scotia
Canada



From daemon Wed Jan 24 14:13:14 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Wed, 24 Jan 2001 13:16:58 -0500
Subject: HT/Spanish/NJ

Contents Retrieved from Microscopy Listserver Archives
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Fellow colleagues, I am posting this for Carlos Saenz, a veteran histotec
and licenced HT, in San Jose, Costa Rica, English is limited, but knowledge
of histotecnology is excellent and commitment is there. He may be reached
at 732-748-069 in New Jersey. I had one reply for Carlos to try and
increase his English skills, and he is trying, but will someone give him an
opportunity.
I have been invited to lecture about Histotecnology in Mexico, Guatemala
and Central America; And, the only countries I have not been in South
America are Venezuela, Colombia, Guyanas, Brazil and Uruguay. And the only
diffrence between the histotecnologist there and in the USA are
opportunities and language barriers, othere than that, knowledge of our
field is excellent.
Thanks again, Teresa




From daemon Wed Jan 24 14:21:36 2001



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Wed, 24 Jan 2001 21:20:48 +0100
Subject: PD: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi
all till now answered people are right - the lens currents are adjusted
according to selected HT by means of sets of analog resistors and reference
voltages adjustable inside the EM400. If this adjustment is not 100%
according to theory (what is allmost 100% true after so many years) than the
image size projected on the screen differs from expected size and mag values
can be different for each HT, non linear thru HT etc.
It is a fine tuning required by service..... but is this mag accuracy worth
this service work ?? maybe just do in series with shots with importance of
mag value the one with same condition and calibration sample inserted for
corrections as reference ??

regards
Krzysztof Herman

EMISJA S.c.
02-892 Warszawa, ul.Bazancia 45 A, Poland
tel/fax: (+48 22)6449753, 6449750
tel: (+48 601)307456
kherman-at-labsoft.com.pl
www.emission.com.pl


----- Wiadomooæ oryginalna -----
Od: Debby Sherman {dsherman-at-purdue.edu}
Do: message to: MSA list {microscopy-at-sparc5.microscopy.com}
Wys³ano: 24 stycznia 2001 15:45
Temat: TEM calibration


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
} I just wanted to run some interesting calibration data by you. We are
working with some small particles and have to have reasonable size
calibrations at both 80 and 100kV on a Philips EM-400 microscope (~20 years
old). Calibration using replica gratings and catalase lattice spacing give
us values somewhat below the magnifications indicated by the mag. readout on
the panel. This is fine. The important thing is that we know what the true
mags are.
} One thing we did find is that the magnifications are consistantly
slightly lower in value at 100kV than at 80kV. This surprised me at first
but I am assuming that the difference is due to the higher acceleration of
the electrons at 100kV. Am I correct to assume that higher energy electrons
would be influenced less by the lenses, resulting in lower x-over point,
which could result in a slight decrease in the magnification?
} I suspect that newer microscopes could compensate for this through the
software programming and will be doing the calibration at different kV
values on our CM-10 to verify this.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-099e Whistler Building
} West Lafayette, IN 47907
}



From daemon Wed Jan 24 15:07:48 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 24 Jan 2001 15:03:00 -0600
Subject: Re: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey, deja-vu all over again......

I encountered a similar (green sludge and corrosion) problem on the
DP's and recirculating water system of our TEM several years ago. I
assumed that it was a reaction between the iron and copper tubing
used in the system. Old time plumbers, for example, always warned
about directly connecting iron and copper tubing because of the
corrosion that would occur. I KNOW that on our DP's the iron fittings
have copper connections. Oh well....

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Jan 24 15:09:22 2001



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Wed, 24 Jan 2001 22:08:57 +0100
Subject: Odp: PD: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Wiadomooæ oryginalna -----
Od: Peggy Bisher {peggy-at-research.nj.nec.com}
Do: Krzysztof Herman {kherman-at-labsoft.com.pl}
Wys³ano: 24 stycznia 2001 21:44
Temat: Re: PD: TEM calibration


} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} } Hi
} } all till now answered people are right - the lens currents are adjusted
} } according to selected HT by means of sets of analog resistors and
reference
} } voltages adjustable inside the EM400. If this adjustment is not 100%
} } according to theory (what is allmost 100% true after so many years) than
the
} } image size projected on the screen differs from expected size and mag
values
} } can be different for each HT, non linear thru HT etc.
} } It is a fine tuning required by service..... but is this mag accuracy
worth
} } this service work ?? maybe just do in series with shots with importance
of
} } mag value the one with same condition and calibration sample inserted for
} } corrections as reference ??
} }
} } regards
} } Krzysztof Herman
} }
} } EMISJA S.c.
} } 02-892 Warszawa, ul.Bazancia 45 A, Poland
} } tel/fax: (+48 22)6449753, 6449750
} } tel: (+48 601)307456
} } kherman-at-labsoft.com.pl
} } www.emission.com.pl
}
}
} I do remember at one time that Philips would guarantee that the
} "nominal mag" be +/- 5% of the actual mag no matter what kV you were
} using. What I am saying is that if you calibrate your microscope and
} you found it to be more or less than 5% of what is actually displayed
} on the panel then they should come in and make an adjustment for
} you, providing you have a maintenance contract.
} Many years ago I found one of my EM400's to be in some instances
} 10-15% out so they fixed it. Otherwise it is your responsibility to
} calibrate your microscope.

I am the service specialist grown from CM series onwards. I was maintening
not only Philips microscopes. But each time I touch the EM-xxx machine I
feel the smell of nobel time of Philips name. Now all this become a
business, very commercial and without soul. Software makes it like this. It
is similar difference like the old mechanical swiss clock 100 years old and
modern Taiwan made electronic one. Do You feel the difference ?
From otehr side the people from the times You mentioned (times of "customer
first" slogan) "they should come and adjust it..." so "they" are allmost all
retired or gone.
I think - be happy that this EM-XXX still works. Ofcourse - pure customers
point of view and feeling can differ from mine.
All the best
Krzysztof Herman
EMISJA S.c.
02-892 Warszawa, ul.Bazancia 45 A,
tel/fax: (+48 22)6449753, 6449750
tel: (+48 601)307456
kherman-at-labsoft.com.pl
www.emission.com.pl



From daemon Wed Jan 24 16:28:30 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Wed, 24 Jan 2001 17:25:28 -0500
Subject: FW: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Debby and Bill,

I suspect (but have not worked out), another possibility could be that the lens
currents are set properly for 100kV but your HT tank is actually providing
somewhat less..or more? Without thinking too hard, it seems like } 100kV could
result in lower than expected mags. Is this error consistent over weeks,
months? My EM300 calibrates consistently higher than published values, but I
haven't seen a systematic difference with kV. In any case, if you have service
on the instrument, ask the engineer to check currents, reference resistors,
etc. The correct values should be easy to find.

Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu



}
}
} Dear Debby,
}
} It is true that the lens currents will be higher at 100 kV than at 80
} kV;
} however, even a 20-year-old microscope takes this into account and sets
} the lens currents to match the accelerating voltage. It seems that in
} your case this correction is slightly off resulting in the
} systematically
} lower mags. The newer microscopes will also set the lens currents to
} match the voltage, but whether this is done through software or
} electromechanically I don't know.
}
} Yours,
}
}
} Bill Tivol
} Wadsworth Center
} Albany NY
} (518) 473-7399 WFT02-at-health.state.ny.us
}



From daemon Wed Jan 24 17:19:57 2001



From: tamara a howard :      thoward-at-UNM.EDU
Date: Wed, 24 Jan 2001 16:16:05 -0700 (MST)
Subject: Image Pro Express evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone using/used in the past Image Pro Express software (or Image Pro
Plus)? I'm unfamiliar with the package, and would like to hear any
comments (+ or -).

Thanks!

Tamara

(note new home bench!)
|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Wed Jan 24 17:37:19 2001



From: John Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 25 Jan 2001 09:34:06 +1000
Subject: Re: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day Frank,
Early last year we had a similar problem on our E3 ESEM, the water lines
were blocking up with gelatinous material. After many attempts to
reverse flush the lines we decided to pull all the water lines off and
replace them. Our problem was in the water cooled alloy heatsink board,
the alloy was slowly dissolving. We reamed out the alloy and fitted
copper tube through the cooling channel. This solved the problem.
Regards
JVN

Frank Thomas wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Listers -
}
} I've just run into a problem (and, I think, partially solved it) that
} may affect a lot of beam instruments with water-cooled diffusion pumps.
} We'd been having some problems lately with at least one of the diff
} pumps on our ESEM shutting itself off because it thought it wasn't being
} cooled enough. I checked our little Neslab chiller unit; it seemed OK, and
} since I'd just replaced the pump in it last year, I figured that there
} really couldn't be a problem with that....
} So I was starting to worry that maybe we had a diff pump or at least a
} sensor starting to fail, but then this morning, the other diff pump also
} shut itself down. Hello, thinks I, then this has to be a cooling problem. So
} I disconnect the outflow from the chiller, and check the flow. Damn, lots of
} water coming out....must be something else. But then I get a bright
} idea....let's check the inline filter. Turns out, the filter is getting a
} bit full of crud. Green crud. Ok, so I put a new one in. Then I get a second
} bright idea (my personal daily limit). Let's check the water pressure/flow
} returning from the ESEM. ....Oh oh, very low. So I disconnect both inlet and
} outlet lines from the ESEM, stick the nozzle of a can of compressed air in
} one, and let fly. Wish I hadn't had the other line aimed at myself just
} then, but that's beside the point. About a half a litre of pretty dirty
} water came shooting out, including a surprising number of little solid bits
} of stuff. Judging by their blue-green colour, they're copper oxides,
} presumably from the water lines in my slowly-decomposing ESEM.
} Anyway, after that system flush, the flow seems a lot better, and now
} the diff pumps seem to be staying on.
} I don't believe it says anywhere in the manual that one should regularly
} do this kind of procedure on one's beam instrument, but I wonder if this is
} a common problem. Maybe some of you do a flush like this on a regular
} (annual?) basis?
} The water in our system is RO, and I don't put anything in to treat for
} algae, since that doesn't seem to be a problem. Yet there's obviously some
} corrosion going on. Is there a way to buffer the water to ensure a neutral
} pH that will certainly not bother the instrument? I'm assuming our water is
} a bit on the acid side, for this corrosion to be happening (?).
}
} F. C. Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Dartmouth, Nova Scotia
} Canada

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Wed Jan 24 18:06:21 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 25 Jan 2001 10:03:53 +1000
Subject: RE: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A law of nature: Small diameter tubes in a heated system crud up.
Filters must be cleaned and anti-corrosion fluids renewed.
Check the flow rate (litres per minute) of the return line every couple of
weeks.
A thermometer measuring the temperature of the returned water is useful.
Return lines from each instrument are best hooked into a 50mm gravity drain,
which obviously must be higher than the cooling water reservoir.
Such an open drain avoids back-pressure and makes monitoring of different
instruments easier.

After some years EMs will need to be reversed flushed with water. If the flow
is still not good enough, than pumping of a weak acid (10% acetic/vinegar)
through the instrument in a loop may be required .
There was a long discussion on anti corrosion items a couple of years ago. The
Tips and Ticks site (one of the first on our Links page) may have that
information more easily accessible than wading through the archives.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, January 25, 2001 5:51 AM, Frank Thomas
[SMTP:thomasf-at-AGC.BIO.NS.CA] wrote:
}
} Listers -
}
} I've just run into a problem (and, I think, partially solved it) that
} may affect a lot of beam instruments with water-cooled diffusion pumps.
} We'd been having some problems lately with at least one of the diff
} pumps on our ESEM shutting itself off because it thought it wasn't being
} cooled enough. I checked our little Neslab chiller unit; it seemed OK, and
} since I'd just replaced the pump in it last year, I figured that there
} really couldn't be a problem with that....
} So I was starting to worry that maybe we had a diff pump or at least a
} sensor starting to fail, but then this morning, the other diff pump also
} shut itself down. Hello, thinks I, then this has to be a cooling problem. So
} I disconnect the outflow from the chiller, and check the flow. Damn, lots of
} water coming out....must be something else. But then I get a bright
} idea....let's check the inline filter. Turns out, the filter is getting a
} bit full of crud. Green crud. Ok, so I put a new one in. Then I get a second
} bright idea (my personal daily limit). Let's check the water pressure/flow
} returning from the ESEM. ....Oh oh, very low. So I disconnect both inlet and
} outlet lines from the ESEM, stick the nozzle of a can of compressed air in
} one, and let fly. Wish I hadn't had the other line aimed at myself just
} then, but that's beside the point. About a half a litre of pretty dirty
} water came shooting out, including a surprising number of little solid bits
} of stuff. Judging by their blue-green colour, they're copper oxides,
} presumably from the water lines in my slowly-decomposing ESEM.
} Anyway, after that system flush, the flow seems a lot better, and now
} the diff pumps seem to be staying on.
} I don't believe it says anywhere in the manual that one should regularly
} do this kind of procedure on one's beam instrument, but I wonder if this is
} a common problem. Maybe some of you do a flush like this on a regular
} (annual?) basis?
} The water in our system is RO, and I don't put anything in to treat for
} algae, since that doesn't seem to be a problem. Yet there's obviously some
} corrosion going on. Is there a way to buffer the water to ensure a neutral
} pH that will certainly not bother the instrument? I'm assuming our water is
} a bit on the acid side, for this corrosion to be happening (?).
}
}
} F. C. Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Dartmouth, Nova Scotia
} Canada
}



From daemon Wed Jan 24 18:49:31 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Wed, 24 Jan 2001 16:51:17 -0800
Subject: teaching microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am assembling platform and poster presentations for a symposium on
"Teaching Microscopy" to be given at the upcoming MSA meeting in Long Beach
from August 5-9, 2001. I encourage anyone with an interest or experience
with this topic to submit a presentation. Please contact me directly for
more information or to answer any questions. See you in Long Beach!

Teaching Microscopy

Remote links to various light and electron microscopes, virtual
microscopes, and CD-ROMS of images are all being used to teach colleagues
and/or students about microscope theory, operation, and utilization. These
are the new hardware and software tools for instruction. Are they
effective? How are these tools being integrated into training programs and
school curricula? What should be included in a training course? What is the
best way to teach these concepts? This symposium will highlight examples of
the hardware and software tools, as well as discuss different approaches,
from formal classes and image collections to intensive workshops, to be
used to teach microscopy theory and operation.

Steve Barlow



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/





From daemon Wed Jan 24 19:12:14 2001



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 24 Jan 2001 18:07:47 -0700
Subject: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use DI water and ethylene glycol or some good old fashioned anti-freeze.
However, I can't remember the concentration right now. I think our
"corrosion" problems promptly disappeared.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Frank Thomas [mailto:thomasf-at-AGC.BIO.NS.CA]
Sent: Wednesday, January 24, 2001 12:51 PM
To: Microscopy-at-sparc5.microscopy.com


Listers -

I've just run into a problem (and, I think, partially solved it) that
may affect a lot of beam instruments with water-cooled diffusion pumps.
We'd been having some problems lately with at least one of the diff
pumps on our ESEM shutting itself off because it thought it wasn't being
cooled enough. I checked our little Neslab chiller unit; it seemed OK, and
since I'd just replaced the pump in it last year, I figured that there
really couldn't be a problem with that....
So I was starting to worry that maybe we had a diff pump or at least a
sensor starting to fail, but then this morning, the other diff pump also
shut itself down. Hello, thinks I, then this has to be a cooling problem. So
I disconnect the outflow from the chiller, and check the flow. Damn, lots of
water coming out....must be something else. But then I get a bright
idea....let's check the inline filter. Turns out, the filter is getting a
bit full of crud. Green crud. Ok, so I put a new one in. Then I get a second
bright idea (my personal daily limit). Let's check the water pressure/flow
returning from the ESEM. ....Oh oh, very low. So I disconnect both inlet and
outlet lines from the ESEM, stick the nozzle of a can of compressed air in
one, and let fly. Wish I hadn't had the other line aimed at myself just
then, but that's beside the point. About a half a litre of pretty dirty
water came shooting out, including a surprising number of little solid bits
of stuff. Judging by their blue-green colour, they're copper oxides,
presumably from the water lines in my slowly-decomposing ESEM.
Anyway, after that system flush, the flow seems a lot better, and now
the diff pumps seem to be staying on.
I don't believe it says anywhere in the manual that one should regularly
do this kind of procedure on one's beam instrument, but I wonder if this is
a common problem. Maybe some of you do a flush like this on a regular
(annual?) basis?
The water in our system is RO, and I don't put anything in to treat for
algae, since that doesn't seem to be a problem. Yet there's obviously some
corrosion going on. Is there a way to buffer the water to ensure a neutral
pH that will certainly not bother the instrument? I'm assuming our water is
a bit on the acid side, for this corrosion to be happening (?).


F. C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Dartmouth, Nova Scotia
Canada



From daemon Wed Jan 24 19:18:39 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Thu, 25 Jan 2001 13:04:39 +1100
Subject: Re: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good question.

I used to put antifreeze in the chillers but in the later models there is a
warning stating not to use antifreeze as it damages the seals.

I would like to know what everyone now uses to prevent organic growth.

By the way the DP's must have been fairly warm to trip the sensors.
If I have any questions at all about the temperture Is usually feel the DP
cooling coils.
All the coils should be cool except for the last two or three turns. Typical
temperature is 20 deg C.

Earl Weltmer
----- Original Message -----
} From: "Frank Thomas" {thomasf-at-AGC.BIO.NS.CA}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 24, 2001 11:51 AM


Earl said:
I used to put antifreeze in the chillers but in the later models there is
a
warning stating not to use antifreeze as it damages the seals.

I would like to know what everyone now uses to prevent organic growth.


We dont put anything in the water these days, but check filters, flush and
reverse flush once a year or whenever convenient or when the temperature or
flow meters look ominous. Our recirculating system has a 500 litre buffer
tank which is cleaned about every five years.

We have had certainly no more trouble, probably less, than when we put
various chemicals into the water

Sally




Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218 or 6125 8525
http://www.anu.edu.au/EMU



From daemon Wed Jan 24 21:04:55 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 24 Jan 2001 22:05:51 -0500
Subject: Sectioning non-decalcified bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I'm trying to obtain 8 um thick sections of non-decalcified bone which
has been embedded in methacrylate using a R-J autocut. Thanks in advance
for any tips to minimize compression and adhere sections to a glass slide.
Rosemary Walsh
EMF for the Life Sciences
Penn State University


From daemon Thu Jan 25 00:50:15 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Thu, 25 Jan 2001 07:44:29 +0100
Subject: Antwort: Microtome room

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Nancy,

all ultramicrotomes I know run either with a standard electrical supply (eg
110 V in the USA, 220 V in many other countries eg here in Austria) or
with a "full range power supply". Our Leica EM UCT and UCR are equipped
with a full range power supply for a voltage input range from "90 - 260
VAC, 50-60 Hz" (as the engineers call it). Some older model you will have
to switch between voltages depending on the voltage you use...

You may want to run additionally a few desktop lights, a LM for looking at
semis before trimming down to a blockface for ultrathins, etc. but this
should be no problem even with electrical features from the 1950`s.

Important when deciding for a UM room: Are you having vibrations in this
room? In which floor is your room? Are you having a concrete floor? and
what is the floor covering made of? These factors all may affect the
stability of your room!

I strongly recommend the use of an antivibration instrument table or AT
LEAST an antivibration base plate for the UM. Do not use a desk or smthg
similar! You can test for vibrations easily by putting a petridish filled
with water (slightly "overfilled" with a convex meniscus) on a table in
your room under a light - you should see NO waves! Slam the door and watch
what happens :-)

And just one more thought REALLY important: Should you ever decide to work
with a cryosectioning equipment: DANGER OF SUFFOCATION!!! Make sure that
you have a good ventilation of your room! 1 litre of liquid nitrogen (LN2)
will produce 700 litres of gaseous nitrogen (GN2). It is odourless and
tasteless and an operator in a small room may suffocate and not even
recognize that he/she is in danger!!!
You may use an oxygen analyzer (range 0 - 25%) for testing ...

Hope that helps you,

Joachim

Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350






From daemon Thu Jan 25 04:40:57 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 25 Jan 2001 09:54:27 +0000
Subject: Re: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Maybe US plumbers are better trained, but UK heating systems are
almost always iron (pressed steel steel) radiators with copper
connecting pipes. Other metals involved are brass valves and
connectors, and lead/tin solder at soldered joints. Systems like that
corrode unless inhibitors are used. The sludge that collects in my
copper/iron central heating system is deep black, like indian ink, not
green.
Is it possible that your green sludge is not copper but algae??
Transparent plastic tubing in the system may allow alagal growth,
provided there is some source of carbon. Bacterial/fungal
decomposition of glycol could provide that.
Chris

----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 24, 2001 9:03 PM


Debby

there are several things that you should consider when checking magnification on a TEM:
First of all most manufacturers will aim for a consistency of about +/-5% accuracy because of the laws of diminishing returns. Also they may have an optimum voltage
Second there will be variations up and down the magnification scale because a balance has been struck between several lenses and when they are excited differently this will be expressed differently.
Third remember that there is a very short working distance between lens polepiece and specimen (just a few millimetres) so if the specimen height varies by +/- 0.2 mm this will greatly affect the magnification. For instance if the grid is slightly bent or kinked, if you don't check magnifications at a standard Z position (eucentric if that's available) then you will get inconsistencies of easily 5%. When I calibrated at eucentric I noticed that all of my
magnifications at 75kv were near or better than +/- 4% whereas one or two previous sets were mostly worse than +/- 5%
Fourth all of the above will change with time as the electronics changes so calibrations, if you're fussy, should be done perhaps every 6 months or year if you're fussy or notice great changes. I'm sure that Steve Chapman would agree that it's an excellent way of monitoring part of the performance of your microscope anyway.
Fifth consider how you measure your calibrations you should always measure the negative never the print (use a calibrated graticule eyepiece or travelling microscope). Prints can vary by 1 or 2% so easily.
Sixth the calibration standards for TEM will be at best +/- 2% accurate unless you've got a certificate that says otherwise. They will often differ e.g. catalase and diffraction grating will give you a slightly different answer.
Seventh don't forget the hysteresis factor. On most microscopes you get best calibration by calibrating at the highest magnification then working down the range because of inherent hysteresis in the electromagnetic lenses. Some manufacturers even used to supply a special button to overcome this on our old AEI 801 it was called standardise magnification.
Eighth I'm slipping I can usually come up with about ten problems.

Never quote magnifications to 3 significant figures it's meaningless. It may sound insurmountable but if you are aware of the problems you can reduce or avoid them. If you have calibrated and see little change between sets then you can put confidence limits on your accuracy. If it's critical that a user needs accuracy then photograph a consecutive shot of a calibration sample under exactly the same conditions.

good luck in your quest for the perfect magnification.

Malcolm

Malcolm Haswell
e.m. unit
University of Sunderland


Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all,
} I just wanted to run some interesting calibration data by you. We are working with some small particles and have to have reasonable size calibrations at both 80 and 100kV on a Philips EM-400 microscope (~20 years old). Calibration using replica gratings and catalase lattice spacing give us values somewhat below the magnifications indicated by the mag. readout on the panel. This is fine. The important thing is that we know what the true mags are.
} One thing we did find is that the magnifications are consistantly slightly lower in value at 100kV than at 80kV. This surprised me at first but I am assuming that the difference is due to the higher acceleration of the electrons at 100kV. Am I correct to assume that higher energy electrons would be influenced less by the lenses, resulting in lower x-over point, which could result in a slight decrease in the magnification?
} I suspect that newer microscopes could compensate for this through the software programming and will be doing the calibration at different kV values on our CM-10 to verify this.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-099e Whistler Building
} West Lafayette, IN 47907



From daemon Thu Jan 25 06:15:15 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 25 Jan 2001 12:11:01 +0000 (GMT Standard Time)
Subject: Re: Antwort: Cleaning Knife Boats?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is no need to scrape off the old wax when reusing
Trufs - just put them on the multiplate and it melts and
gets recycled. I used to to re-use them then I hit a
problem where I had difficulty wetting the knife edge. I
got so desperate I decided to use a new boat each time.

Dave


On Wed, 24 Jan 2001 12:51:47 +0100
"joachim.prutsch-at-leica-microsystems.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi list,
}
} may I just add to the discussion about the knife boats:
}
} yes, if you use dental wax to glue the Truf to the knife (with the Leica
} Multiplate, especially designed for that purpose its really easy) the boat
} can be reused a few times.
}
} HOWEVER:
} 1. breaking off the Truf from the knife and scratching away the old
} hardened wax may damage the Truf - leaking may result from this! Can be
} frustrating if you have perfect sections of a precious sample and see them
} dive away ...
} 2. Esp. with ultrathins (but also with semithins) you must be aware of
} possible cross contamination of old sections to the new set (I have no idea
} if there is a GLP rule on that but in a hospital mixing up different
} samples might cause serious problems)!
}
} BTW the "LKB"Trufs are "Leica"Trufs now, but call them as you like it :-)
}
} Best regards,
}
} Joachim
}
} Dr. Joachim Prutsch
} Product Manager EM Specimen Preparation
}
} Leica Microsystems GmbH
} Hernalser Hauptstr. 219 email:
} Joachim.Prutsch-at-leica-microsystems.com
} A 1170 Vienna Tel.: +43 1 4 88 99 - 235
} AUSTRIA Fax: +43 1 4 88 99 - 350
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Jan 25 07:12:56 2001



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Thu, 25 Jan 2001 13:07:59 +0000
Subject: TEM magnification calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One point about magnification calibration that I have not read here yet
is that for a particular nominal setting, the actual mag differs when
the mag has been stepped up compared to stepping down the mag. I have
been told this is due to hysteresis in the lenses (predominantly the
objective probably). I have measured up to 10% difference.

I have not kept a close eye on this discussion, but the dependence of
the electron voltage was mentioned. If this is so, that means that the
electron accelerating voltage would need to be checked. I remember in
the book by Forwood and Clarebrough (Electron Microscopy of Interfaces
in Metals and Alloys, published by Adam Hilger) where they found the
accelerating voltage of their instrument was 220 keV as opposed to 200
keV. I take it that could make a difference.


--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Thu Jan 25 07:57:14 2001



From: Leslie Eibest :      leibest-at-duke.edu
Date: Thu, 25 Jan 2001 09:01:43 -0500
Subject: Re: fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 5:16 PM -0800 1/24/01, Earl Weltmer wrote:
} I would like to know what everyone now uses to prevent organic growth.


We sprinkle dichlorophene on the surface of the water in our Haskris
chiller to prevent fungal/bacterial growth, and buffer the water to
about pH 7.0 with sodium bicarbonate. So far (for the last 21
years), these procedures have worked fine.

Leslie Eibest


From daemon Thu Jan 25 08:04:20 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Thu, 25 Jan 2001 09:02:07 -0500
Subject: Image Pro Express evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes Tamara,

I used the working demo version of Image Pro Plus during the summer to help
analyze my SEM photos for my masters thesis. I found the program to be very
easy to use and very functional. I was really pleased with how well you
could set, save, and recall image calibrations for doing measurement work.
I had SEM micrographs from two different machines (one acquired digitally,
the other Polaroid film) and each had a different screen calibration.

dz

-----Original Message-----
} From: tamara a howard [mailto:thoward-at-UNM.EDU]
Sent: Wednesday, January 24, 2001 6:16 PM
To: Microscopy-at-sparc5.microscopy.com; histonet-at-pathology.swmed.edu


Is anyone using/used in the past Image Pro Express software (or Image Pro
Plus)? I'm unfamiliar with the package, and would like to hear any
comments (+ or -).

Thanks!

Tamara

(note new home bench!)
|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Thu Jan 25 08:18:02 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 25 Jan 2001 09:16:06 -0500
Subject: RE: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl,

I heard the same about the antifreeze...checked with Haskris and they
recommended (a year or two ago) Dichlorophene to prevent biological growth.
Seems to work but I still need to clean filters 2-3 times a year and monitor
flow. Haskris actually faxed me an info sheet, there's a chance I could find
it so email me if you would like it.

Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Wednesday, January 24, 2001 8:16 PM, Earl Weltmer [SMTP:eweltmer-at-home.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good question.
}
} I used to put antifreeze in the chillers but in the later models there is a
} warning stating not to use antifreeze as it damages the seals.
}
} I would like to know what everyone now uses to prevent organic growth.
}
} By the way the DP's must have been fairly warm to trip the sensors.
} If I have any questions at all about the temperture Is usually feel the DP
} cooling coils.
} All the coils should be cool except for the last two or three turns. Typical
} temperature is 20 deg C.
}
} Earl Weltmer
} ----- Original Message -----
} } From: "Frank Thomas" {thomasf-at-AGC.BIO.NS.CA}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, January 24, 2001 11:51 AM
} Subject: Fun with vacuum systems
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Listers -
} }
} } I've just run into a problem (and, I think, partially solved it) that
} } may affect a lot of beam instruments with water-cooled diffusion pumps.
} } We'd been having some problems lately with at least one of the diff
} } pumps on our ESEM shutting itself off because it thought it wasn't being
} } cooled enough. I checked our little Neslab chiller unit; it seemed OK, and
} } since I'd just replaced the pump in it last year, I figured that there
} } really couldn't be a problem with that....
} } So I was starting to worry that maybe we had a diff pump or at least a
} } sensor starting to fail, but then this morning, the other diff pump also
} } shut itself down. Hello, thinks I, then this has to be a cooling problem.
} So
} } I disconnect the outflow from the chiller, and check the flow. Damn, lots
} of
} } water coming out....must be something else. But then I get a bright
} } idea....let's check the inline filter. Turns out, the filter is getting a
} } bit full of crud. Green crud. Ok, so I put a new one in. Then I get a
} second
} } bright idea (my personal daily limit). Let's check the water pressure/flow
} } returning from the ESEM. ....Oh oh, very low. So I disconnect both inlet
} and
} } outlet lines from the ESEM, stick the nozzle of a can of compressed air in
} } one, and let fly. Wish I hadn't had the other line aimed at myself just
} } then, but that's beside the point. About a half a litre of pretty dirty
} } water came shooting out, including a surprising number of little solid
} bits
} } of stuff. Judging by their blue-green colour, they're copper oxides,
} } presumably from the water lines in my slowly-decomposing ESEM.
} } Anyway, after that system flush, the flow seems a lot better, and now
} } the diff pumps seem to be staying on.
} } I don't believe it says anywhere in the manual that one should
} regularly
} } do this kind of procedure on one's beam instrument, but I wonder if this
} is
} } a common problem. Maybe some of you do a flush like this on a regular
} } (annual?) basis?
} } The water in our system is RO, and I don't put anything in to treat
} for
} } algae, since that doesn't seem to be a problem. Yet there's obviously some
} } corrosion going on. Is there a way to buffer the water to ensure a neutral
} } pH that will certainly not bother the instrument? I'm assuming our water
} is
} } a bit on the acid side, for this corrosion to be happening (?).
} }
} }
} } F. C. Thomas
} } MicroAnalysis Facility
} } Geological Survey of Canada (Atlantic)
} } Dartmouth, Nova Scotia
} } Canada
} }
} }



From daemon Thu Jan 25 08:45:16 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Thu, 25 Jan 2001 09:41:55 -0500
Subject: black & white print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
Our 10 year old Ilford print processor is breaking down piece by
piece. We've about reached the point of diminishing returns and so are
considering replacing it. Not everyone has switched to digital so we
use quite a lot of film. Can any of you recommend a good black and white
print processor?

Thank you,

Mary Gail Engle
Manager
Electron Microscopy & Imaging Facility
University of Kentucky


From daemon Thu Jan 25 10:45:43 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 25 Jan 2001 10:40:30 -0600
Subject: Salary Range for Microscopist at University

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We need to determine a reasonable salary (at a research university)
for someone with the following skills:

PhD degree in biological sciences
11 yr experience (practial & theory) in LM, SEM, TEM
microscope maintenance (basic and advanced-mechanical)
basic and advanced scope operator (STEM, EDS)
excellent training skills (personable)
excellent communication skills (verbal, written)
specimen prep, ultramicrotomy, immunocytochemistry, vacuum
techniques, digital imaging, statistics
1 yr exerience in atomic force microscopy
8 yr supervisory experience

This is a 12 month, continuing, non-tenured, hard-money position.
The person provides research support to faculty & researchers on campus.

What are other academic institutuions paying such (or similar) individuals?

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Jan 25 12:32:50 2001



From: DrJohnRuss-at-aol.com
Date: Thu, 25 Jan 2001 13:27:53 EST
Subject: Ann: Image Processing and Measurement Workshop

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The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ through the North Carolina State University Department
of Continuing and Professional Education is now in its 19th year. The course
will be presented May 9 - 11, 2001, in Raleigh, NC, and June 6-8, 2001, at
the Danish Technological Institute in Taastrup, Denmark (near Copenhagen).
This course has generated highly favorable reviews from the thousands of
previous students. The primary focus is on images from various types of
microscopy, with practical guidance in correcting imaging defects, enhancing
the images for presentation and measurement, and performing stereological
meaningful measurements on them. Textbooks and computer software are provided
to attendees. Lab sessions with an opportunity to bring your own images makes
this course immediately useful and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.AOL.com/IPCourse/




From daemon Thu Jan 25 12:38:28 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Thu, 25 Jan 2001 13:42:45 -0500
Subject: SEM of stomata

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Hi all,
Is it possible to fix plant tissue and have the stoma (guard cells and the
pore between them) remain in their original position (opened or closed)?
Does anyone have a reference?
thanks for the help,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Thu Jan 25 13:37:12 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Thu, 25 Jan 2001 13:34:04 -0600
Subject: Re: Salary Range for Microscopist at University

Contents Retrieved from Microscopy Listserver Archives
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Hello John
Surely you will hear of people making less or more but between the survey
done by Don Grimes of Microscopy Today (457 microscopist) & the salary survey
of optically educated individuals published annually in Photonics Spectra, I
think your window is 50-70K$. Can you get someone for less, probably. Would you
like continuity in the years to come? (the answer is yes) One more thing, I
don't think the range of skills & experience you are asking for are typical of
microscopist in education e.g. ignore the educational discount usually applied
to most of us.

Bruce Brinson
Rice U.

"John J. Bozzola" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We need to determine a reasonable salary (at a research university)
} for someone with the following skills:
}
} PhD degree in biological sciences
} 11 yr experience (practial & theory) in LM, SEM, TEM
} microscope maintenance (basic and advanced-mechanical)
} basic and advanced scope operator (STEM, EDS)
} excellent training skills (personable)
} excellent communication skills (verbal, written)
} specimen prep, ultramicrotomy, immunocytochemistry, vacuum
} techniques, digital imaging, statistics
} 1 yr exerience in atomic force microscopy
} 8 yr supervisory experience
}
} This is a 12 month, continuing, non-tenured, hard-money position.
} The person provides research support to faculty & researchers on campus.
}
} What are other academic institutuions paying such (or similar) individuals?
}
} Thank you.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ##############################################################



From daemon Thu Jan 25 14:45:55 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Thu, 25 Jan 2001 12:47:03 -0800
Subject: teaching microscopy CD-ROMS

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Dear Barry

I'm sure there are more microscopy CD-ROMS out there than I know about. A
very nice one that comes to mind is one produced by Oxford Instruments as
part of its X-Ray microanalysis tutorial. That one concentrates mainly on
SEM and microanalysis. It was on desplay at recent MSA meetings.
Instructors are best served by going to the Project Micro website and
checking that bibliography, so ably maintained by the tireless Caroline
Schooley. If anyone knows about microscopy materials not on that list,
please send me and Caroline the title and source (even better a review of
the material and what level it is appropriate for).

While the major emphasis of Project Micro is for pre-college, Caroline has
tried to include useful materials for all levels.

Steve

} From: "Barry Searle" {B.Searle-at-unsw.edu.au}
} To: "Steve Barlow" {sbarlow-at-sunstroke.sdsu.edu}
} Subject: Re: teaching microscopy
} Date: Thu, 25 Jan 2001 12:13:46 +1100
} MIME-Version: 1.0
} X-Priority: 3} Steve,
}
} Would you have a web address for CD-ROMS on electron Microscopy and
} teaching?
}
} Thanks
}
} Barry
} EMU
} UNSW
}
}
} ----- Original Message -----
} From: Steve Barlow {sbarlow-at-sunstroke.sdsu.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, January 25, 2001 11:51 AM
} Subject: teaching microscopy
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I am assembling platform and poster presentations for a symposium on
} } "Teaching Microscopy" to be given at the upcoming MSA meeting in Long
} Beach
} } from August 5-9, 2001. I encourage anyone with an interest or experience
} } with this topic to submit a presentation. Please contact me directly for
} } more information or to answer any questions. See you in Long Beach!
} }
} } Teaching Microscopy
} }
} } Remote links to various light and electron microscopes, virtual
} } microscopes, and CD-ROMS of images are all being used to teach colleagues
} } and/or students about microscope theory, operation, and utilization. These
} } are the new hardware and software tools for instruction. Are they
} } effective? How are these tools being integrated into training programs and
} } school curricula? What should be included in a training course? What is
} the
} } best way to teach these concepts? This symposium will highlight examples
} of
} } the hardware and software tools, as well as discuss different approaches,
} } from formal classes and image collections to intensive workshops, to be
} } used to teach microscopy theory and operation.
} }
} } Steve Barlow
} }
} }
} }
} } ___________________________________________________
} } Dr. Steven Barlow
} } EM Facility/Biology Dept.
} } San Diego State University
} } 5500 Campanile Drive
} } San Diego CA 92182-4614
} } phone: (619) 594-4523
} } fax: (619) 594-5676
} }
} } email: sbarlow-at-sunstroke.sdsu.edu
} } http://www.sci.sdsu.edu/emfacility
} }
} } Chairman, Educational Outreach subcommittee
} } promoting microscopy instruction and increased access to microscopes
} } Microscopy Society of America
} } http://www.msa.microscopy.com/
} }
} }
} }
} }
} }



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/





From daemon Thu Jan 25 15:47:33 2001



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 25 Jan 2001 15:40:20 -0800
Subject: ETEC module

Contents Retrieved from Microscopy Listserver Archives
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We have an ETEC Autoscan whose main use is as a teaching scope and for
doing demos for the various groups that tour our facility. It has TV
capability which is really helpful for the above mentioned activities,
but is also nice when you're searching for small particulates scattered
all over the plug. Our TV Scan module is currently in for repair so we
are without this useful accessory (and the class has started and the
tours keep coming). We have two other ETECs that we use as spare parts,
but neither of them had TV capability. Is there anybody out there
having one of these (working) modules that would be willing to part with
it? We might even be able to work out a trade if I have a module that
you need. TIA.
Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Thu Jan 25 16:30:44 2001



From: Julaine Roffers :      roffers-at-uwm.edu
Date: Thu, 25 Jan 2001 16:29:05 -0600
Subject: problems using tannic acid for SEM

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I have been following a protocol that involves using tannic acid (1% in
50mM Na cacodylate buffer) to prevent shrinkage of cells in Xenopus. I
am looking at cilia on the surface of the embryos and when I incubate in
tannic acid I see a deposition of crystals on the surface of the
embryos. I have made sure that the embryos as well as the solution is
at room temperature before and during incubation but this problem
persists. I am also washing subsequently in cacodylate buffer three
times. Has anyone seen this before or have any suggestions about
preventing it? The tannic acid does appear to reduce cell shrinkage but
makes it difficult to observe the cilia.
Julaine Roffers
University of Wisconsin-Milwaukee


From daemon Thu Jan 25 16:35:27 2001



From: Yali Tang :      ytang-at-anl.gov
Date: 25 Jan 01 16:32:50 -0600
Subject: Re: Image Pro Express evaluation

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"David SBCCOM(N)\"" {David.Ziegler-at-Natick.Army.Mil} ,
Yali Tang {ytang-at-anl.gov}
X-Mailer: QuickMail Pro 1.5.4 (Mac)
X-Priority: 3
Reply-To: Yali Tang {ytang-at-anl.gov}
MIME-Version: 1.0
Content-Transfer-Encoding: 7bit
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From: Yali Tang :      ytang-at-anl.gov
Date: 25 Jan 01 16:32:50 -0600
Subject: Re: Image Pro Express evaluation

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



I used to use Image Pro for image analysis and diffraction pattern
measurements. It is pretty good. Sometime you have to make calibration in order
to measure diffraction patterns. i hope there will be a version for Mac. (
i am now using NIH on Mac)

Yali Tang

Materials Science Division
Argonne National Laboratory
Bldg. 223, A109
Argonne, IL 60439
Ph: 630-252-6219
Fax:630-252-7777
Email:ytang-at-anl.gov



From daemon Thu Jan 25 16:42:50 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 25 Jan 2001 17:41:30 -0500
Subject: RE: Fun with vacuum systems - Dichlorophene

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Listmembers,

For those interested in what Haskris recommended to me: I scanned in their
sheet and OCR'd it so I might have missed some "typos". Other standard
disclaimers: this info is two years old so you may want to check again with
Haskris, or whichever manufacturer applies in your case.

Matt

WATER TREATMENT FOR BIOLOGICAL GROWTH
In reference to our recent phone conversation, following are some suggestions
regarding water treatment for biological growth. Please keep in mind that any
additive must have the prior approval of the manufacturer of the equipment
being cooled.

It is important that you fill the system with clean, potable distilled water.

Check the system after one week of operation, if algae is starting to form,
flush the system in accordance with one of the following alternatives:

ALTERNATIVE 1:
Add one cup of chlorine bleach per 15 gallons of water, circulate 20-30
minutes. Drain the system and refill with clean water.

ATLERNATIVE 2:
Add one pint of hydrogen peroxide per 15 gallons of water. Circulate 20-30
minutes. Drain the system and refill with clean water.

Once the system has been flushed we recommend that you proceed with one of the
following water additives:

ALTERNATIVE 1:
A 10% solution of lab grade (no additives) ethylene glycol is recommended.

ALTERNATIVE 2:
Dichlorophene. This is a non-soluble white powder. It is a
fungicide/bactericide and should be sprinkled evenly across the entire tank's
water surface.

Haskris Co. does not sell this product, but we have the following source for
you to call:

ICN Biomedicals, Inc,. P.O. Box 5023, Costa Mesa, CA 92626 Phone:
800-854-0530

Dichlorophene Catalog # 05201981

ALTERNATIVE 3:
Chlorine, 8 parts per million.


NOTE: A 5 micron filter is very helpful in keeping the system clean, especially
if used in conjunction with one of the additives described above.

If you have any questions, please give us a call.





From daemon Thu Jan 25 16:45:16 2001



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Thu, 25 Jan 2001 16:41:56 -0600
Subject: Immuno gold

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I have a researcher who needs an unusual type of gold-conj. secondary
antibody. Her primary is made in goat and the only known available
anti-goat is made in rabbit. Her tissue has cross-reactivity with
rabbit. Is there any other species (donkey, dog, Brazilian opossum...)
that anyone knows of as an anti-goat gold conjugate secondary? Do we have
to make a home-made version?
Thanks!

Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Thu Jan 25 16:49:11 2001



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Thu, 25 Jan 2001 16:46:54 -0600
Subject: Re: teaching microscopy CD-ROMS

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There is also a very good set of teaching tools for SEM, ESEM, and SEM/EDS done
by Brendan Griffin (UWestern Australia - Perth), Clive Nockolds (UofSydney)
it is University level. Contact Brendan for details at {bjg-at-cyllene.uwa.edu.au}


Nestor
Your Friendly Neighborhood SysOp

===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================




From daemon Thu Jan 25 18:53:45 2001



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Thu, 25 Jan 2001 18:46:10 -0600
Subject: AO 923 knife sharpener

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Does anyone have a manual for a AO 923 knife sharpener that they would be
willing to copy and mail or fax to me? This is the model that
automatically sets the plate heights for the coarse and fine blade facets.


Thank you,


Jaclynn Lett, Research Assistant jlett-at-cid.wustl.edu


Central Institute for the Deaf
Faye and Carl Simons Center for Biology of Hearing and Deafness
4560 Clayton Ave.
St. Louis, MO 63110


voice: 314-977-0257
fax: 314-977-0030







From daemon Thu Jan 25 18:53:47 2001



From: jekstrom-at-mediaone.net
Date: Thu, 25 Jan 2001 18:49:00 -0600
Subject: Ask-A-Microscopist: Zeiss Jena microscope

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Colleagues...

Can anyone help this person . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------------------------------

Email: jekstrom-at-mediaone.net
Name: jim Ekstrom

School: Phillips Exeter Academy

State: NH

Zip: 03833

Question: I have a Zeiss Jena microscope drawing set (Nr.6454) Stamped
Zeichenapparat on the box. I am looking for directions for this particular
instrument or a similar type.

---------------------------------------------------------------------------




From daemon Thu Jan 25 19:28:16 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 25 Jan 2001 19:24:03 -0600
Subject: FW: Fun with vacuum systems

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Fellow Microscopists:
As some one on the metallurgical side of things, I may be able to
help. Chris Jeffry is on the right track. The corrosion product from Cu/Fe
couples. such Cu pipe and Fe fittings, may be magnetite, a black, magnetic
residue or, maybe, red rust but not green Cu compounds. In such a couple, Fe
corrodes and the Cu does not. I'd bet on algae as the green material. You
biologists ought to be able to tell if it's algae.

regards,

Sam Purdy
Technical Center
National Steel Corp.

} ----------
} From: Chris Jeffree
} Sent: Thursday, January 2001, 4:49 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Fun with vacuum systems
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Maybe US plumbers are better trained, but UK heating systems are
} almost always iron (pressed steel steel) radiators with copper
} connecting pipes. Other metals involved are brass valves and
} connectors, and lead/tin solder at soldered joints. Systems like that
} corrode unless inhibitors are used. The sludge that collects in my
} copper/iron central heating system is deep black, like indian ink, not
} green.
} Is it possible that your green sludge is not copper but algae??
} Transparent plastic tubing in the system may allow alagal growth,
} provided there is some source of carbon. Bacterial/fungal
} decomposition of glycol could provide that.
} Chris
}
} ----- Original Message -----
} } From: "John J. Bozzola" {bozzola-at-siu.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, January 24, 2001 9:03 PM
} Subject: Re: Fun with vacuum systems
}
}
} } --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} ---.
} }
} }
} } Hey, deja-vu all over again......
} }
} } I encountered a similar (green sludge and corrosion) problem on the
} } DP's and recirculating water system of our TEM several years ago. I
} } assumed that it was a reaction between the iron and copper tubing
} } used in the system. Old time plumbers, for example, always warned
} } about directly connecting iron and copper tubing because of the
} } corrosion that would occur. I KNOW that on our DP's the iron
} fittings
} } have copper connections. Oh well....
} }
} } JB
} }
} } --
} } ##############################################################
} } John J. Bozzola, Ph.D., Director
} } I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} } 750 Communications Drive - MC 4402
} } Southern Illinois University
} } Carbondale, IL 62901 U.S.A.
} } Phone: 618-453-3730
} } Fax: 618-453-2665
} } Email: bozzola-at-siu.edu
} } Web: http://www.siu.edu/departments/shops/cem.html
} } ##############################################################
} }
}
}




From daemon Fri Jan 26 01:07:30 2001



From: Nazlia Samodien :      ctssamodien-at-samiot.uct.ac.za
Date: Fri, 26 Jan 2001 09:00:23 +0200
Subject: CPD

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Hi All
Is there anybody out there who may have a spare Critical Point Drier to
sell. We urgently need one, and our existing budget does not allow the
purchase of a new one right now. Any offers???? We're hoping that someone
in South Africa would respond to this offer. Makes life a bit easier.
Thanks


_____________________________________________________

Nazlia Samodien

Cardiovascular Research Unit
Dept. of Cardiothoracic Surgery
University of Cape Town Medical School
Anzio Rd, Observatory, 7925, South Africa

Tel. +27 21 406 6398 (office)
+27 21 406 6476 (Secretary)
Fax + 27 21 448 5935


Email: ctssamodien-at-samiot.uct.ac.za










From daemon Fri Jan 26 03:37:41 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Fri, 26 Jan 2001 09:32:19 +0000 (GMT)
Subject: Re: TEM calibration

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Hi Malcolm,
just thought I'd raise a few points. I should say I'm using a 20 year old JEOL 120CX and things may be different for more modern instruments!

1) I find that magnification is changed only slightly by variations in sample position (maybe 3% top to bottom of my 1 mm z-range). However diffraction pattern magnification (camera length) is changed enormously (+/- 50%). It's easy to see why if you draw a ray diagram.
2) I agree the standards are no better than +/- 2%. I'm lucky to be working in a field where I can generate my own standards which are an order of magnitude better (III-V superlattices which can be measured by high resolution X-ray diffraction very accurately).
3) If I turn all the knobs to the end and back I find magnification reproducibility is better than I can measure (about 0.5%). Unless, of course the reference batteries start going flat, but then the position of the knobs in the standard setting changes. I am happy to quote mags to 3 significant figures (but not 4!).

Richard

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} -----------------------------------------------------------------------.
}
}
} Debby
}
} there are several things that you should consider when checking magnification on a TEM:
} First of all most manufacturers will aim for a consistency of about +/-5% accuracy because of the laws of diminishing returns. Also they may have an optimum voltage
} Second there will be variations up and down the magnification scale because a balance has been struck between several lenses and when they are excited differently this will be expressed differently.
} Third remember that there is a very short working distance between lens polepiece and specimen (just a few millimetres) so if the specimen height varies by +/- 0.2 mm this will greatly affect the magnification. For instance if the grid is slightly bent or kinked, if you don't check magnifications at a standard Z position (eucentric if that's available) then you will get inconsistencies of easily 5%. When I calibrated at eucentric I noticed that all of my
} magnifications at 75kv were near or better than +/- 4% whereas one or two previous sets were mostly worse than +/- 5%
} Fourth all of the above will change with time as the electronics changes so calibrations, if you're fussy, should be done perhaps every 6 months or year if you're fussy or notice great changes. I'm sure that Steve Chapman would agree that it's an excellent way of monitoring part of the performance of your microscope anyway.
} Fifth consider how you measure your calibrations you should always measure the negative never the print (use a calibrated graticule eyepiece or travelling microscope). Prints can vary by 1 or 2% so easily.
} Sixth the calibration standards for TEM will be at best +/- 2% accurate unless you've got a certificate that says otherwise. They will often differ e.g. catalase and diffraction grating will give you a slightly different answer.
} Seventh don't forget the hysteresis factor. On most microscopes you get best calibration by calibrating at the highest magnification then working down the range because of inherent hysteresis in the electromagnetic lenses. Some manufacturers even used to supply a special button to overcome this on our old AEI 801 it was called standardise magnification.
} Eighth I'm slipping I can usually come up with about ten problems.
}
} Never quote magnifications to 3 significant figures it's meaningless. It may sound insurmountable but if you are aware of the problems you can reduce or avoid them. If you have calibrated and see little change between sets then you can put confidence limits on your accuracy. If it's critical that a user needs accuracy then photograph a consecutive shot of a calibration sample under exactly the same conditions.
}
} good luck in your quest for the perfect magnification.
}
} Malcolm
}
} Malcolm Haswell
} e.m. unit
} University of Sunderland
}
}
} Debby Sherman wrote:
}
} } ------------------------------------------------------------------------
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} } -----------------------------------------------------------------------.
} }
} } Hi all,
} } I just wanted to run some interesting calibration data by you. We are working with some small particles and have to have reasonable size calibrations at both 80 and 100kV on a Philips EM-400 microscope (~20 years old). Calibration using replica gratings and catalase lattice spacing give us values somewhat below the magnifications indicated by the mag. readout on the panel. This is fine. The important thing is that we know what the true mags are.
} } One thing we did find is that the magnifications are consistantly slightly lower in value at 100kV than at 80kV. This surprised me at first but I am assuming that the difference is due to the higher acceleration of the electrons at 100kV. Am I correct to assume that higher energy electrons would be influenced less by the lenses, resulting in lower x-over point, which could result in a slight decrease in the magnification?
} } I suspect that newer microscopes could compensate for this through the software programming and will be doing the calibration at different kV values on our CM-10 to verify this.
} } Debby
} }
} } Debby Sherman Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-099e Whistler Building
} } West Lafayette, IN 47907
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
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From daemon Fri Jan 26 04:13:17 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 26 Jan 2001 10:10:33 +0000
Subject: Re: TEM calibration

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Dear all

I wrote a throw away comment at the end of my last mailing to the list
about NEVER quoting magnifications to 3 significant figures. I think I
should qualify that
because 3 significant figures at 10k is different from 3 significant
figures at 98k. It might be reasonable to quote 10.5k (this would depend
on what % error your
calibration gave) but pointless to quote 98.5k.

Malcolm


Malcolm Haswell wrote:

}
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America
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-----------------------------------------------------------------------.

}
} Debby
}
} there are several things that you should consider when checking
magnification on a TEM:

} {SNIP}
}
} Never quote magnifications to 3 significant figures it's meaningless.
It may sound insurmountable but if you are aware of the problems you can
reduce
or avoid them. If you have calibrated and see little change between sets
then you can put confidence limits on your accuracy. If it's critical
that a user
needs accuracy then photograph a consecutive shot of a calibration
sample under exactly the same conditions.
}
} good luck in your quest for the perfect magnification.
}
} Malcolm
}
} Malcolm Haswell
} e.m. unit
} University of Sunderland
}
} Debby Sherman wrote:
}
} }
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} }
-----------------------------------------------------------------------.

} }
} } Hi all,
} } I just wanted to run some interesting calibration data by you.
We are working with some small particles and have to have reasonable
size
calibrations at both 80 and 100kV on a Philips EM-400 microscope (~20
years old). Calibration using replica gratings and catalase lattice
spacing give
us values somewhat below the magnifications indicated by the mag.
readout on the panel. This is fine. The important thing is that we
know what the
true mags are.
} } One thing we did find is that the magnifications are consistantly
slightly lower in value at 100kV than at 80kV. This surprised me at
first but I am
assuming that the difference is due to the higher acceleration of the
electrons at 100kV. Am I correct to assume that higher energy electrons
would be
influenced less by the lenses, resulting in lower x-over point, which
could result in a slight decrease in the magnification?
} } I suspect that newer microscopes could compensate for this
through the software programming and will be doing the calibration at
different kV
values on our CM-10 to verify this.
} } Debby
} }
} } Debby Sherman Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-099e Whistler Building
} } West Lafayette, IN 47907



From daemon Fri Jan 26 07:08:40 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 26 Jan 2001 04:57:53 -0800 (PST)
Subject: Re: problems using tannic acid for SEM

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Julaine:
A question: how are you using the tannic acid? Is this in one of the OTOTO
methods, or just part of the glut mixture? An answer to these would allow
me to give you some advice/experience, as I have used both, but they are
really different issues. I'll check back later today, and try to help then.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Thu, 25 Jan 2001 16:29:05 -0600, Julaine Roffers wrote:

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} -----------------------------------------------------------------------.
}
}
} I have been following a protocol that involves using tannic acid (1% in
} 50mM Na cacodylate buffer) to prevent shrinkage of cells in Xenopus. I
} am looking at cilia on the surface of the embryos and when I incubate in
} tannic acid I see a deposition of crystals on the surface of the
} embryos. I have made sure that the embryos as well as the solution is
} at room temperature before and during incubation but this problem
} persists. I am also washing subsequently in cacodylate buffer three
} times. Has anyone seen this before or have any suggestions about
} preventing it? The tannic acid does appear to reduce cell shrinkage but
} makes it difficult to observe the cilia.
} Julaine Roffers
} University of Wisconsin-Milwaukee
}





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From daemon Fri Jan 26 07:28:32 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 26 Jan 2001 23:26:45 +1000
Subject: RE: Antwort: Microtome room

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I used nine ultramicrotomes (including a cryo) over the years. All except for
one Reichert, which had its own desk, were placed on a common office desk and
we never had a hint of vibration problems.
(Who remembers the hand-driven Porter-Blum, the Siroflex and the Huxley, great
stuff - 35 years ago) However, my labs were either on the ground floor or in a
basement. I suggest that you buy an expensive table only when needed.

Suffocation: sure its possible, but why scare the population. Our body does not
require the nominal 16% of O2. The partial pressure of gases dictates that only
about half the nominal is available at 10,000 ft altitude, which is equivalent
to the cabin pressure of aircraft at high altitude.
A small microtome room of 4x3x2.5m contains 30,000 liters of air and over 80%
of that is nitrogen. I don't know how nitrogen hungry your equipment is, but I
suggest that leaving the door open is good advice. If you have air-conditioning
with return vents, you can even shut that door.
I rather have a Canary than an oxygen analyser in the cryolab.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, January 25, 2001 4:44 PM, "joachim.prutsch-at-leica-microsystems.com"
-at-sparc5.microscopy.com
[SMTP:"joachim.prutsch-at-leica-microsystems.com"-at-sparc5.microscopy.com] wrote:
}
} Hi Nancy,
}

snip}
} Important when deciding for a UM room: Are you having vibrations in this
} room? In which floor is your room? Are you having a concrete floor? and
} what is the floor covering made of? These factors all may affect the
} stability of your room!
}
} I strongly recommend the use of an antivibration instrument table or AT
} LEAST an antivibration base plate for the UM. Do not use a desk or smthg
} similar! You can test for vibrations easily by putting a petridish filled
} with water (slightly "overfilled" with a convex meniscus) on a table in
} your room under a light - you should see NO waves! Slam the door and watch
} what happens :-)
}
} And just one more thought REALLY important: Should you ever decide to work
} with a cryosectioning equipment: DANGER OF SUFFOCATION!!! Make sure that
} you have a good ventilation of your room! 1 litre of liquid nitrogen (LN2)
} will produce 700 litres of gaseous nitrogen (GN2). It is odourless and
} tasteless and an operator in a small room may suffocate and not even
} recognize that he/she is in danger!!!
} You may use an oxygen analyzer (range 0 - 25%) for testing ...
}
} Hope that helps you,
}
} Joachim
}
} Dr. Joachim Prutsch
} Product Manager EM Specimen Preparation
}
} Leica Microsystems GmbH
} Hernalser Hauptstr. 219 email:
} Joachim.Prutsch-at-leica-microsystems.com
} A 1170 Vienna Tel.: +43 1 4 88 99 - 235
} AUSTRIA Fax: +43 1 4 88 99 - 350
}
}
}
}



From daemon Fri Jan 26 07:35:56 2001



From: jshields-at-cb.uga.edu
Date: Fri, 26 Jan 2001 08:37:28 -0500
Subject: Re: Immuno gold sources

Contents Retrieved from Microscopy Listserver Archives
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I have found that Accurate (don't have the address handy,
somewhere in NY state if I remember) has a wide variety of oddball
antibody, sera, and toxin inventory.
Also the website http://www.antibodygateway.com/
is sort of a clearing house for several companies.
john

On 25 Jan 2001, at 16:41, Tracey M. Pepper wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} I have a researcher who needs an unusual type of gold-conj. secondary
} antibody. Her primary is made in goat and the only known available
} anti-goat is made in rabbit. Her tissue has cross-reactivity with
} rabbit. Is there any other species (donkey, dog, Brazilian
} opossum...) that anyone knows of as an anti-goat gold conjugate
} secondary? Do we have to make a home-made version? Thanks!
}
} Tracey Pepper
} Supervisor
} Bessey Microscopy Facility
} Iowa State University
} ph: 515-294-3872
} fax: 515.294.1337
}




From daemon Fri Jan 26 08:21:12 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 26 Jan 2001 10:26:13 -0400
Subject: Fun with....revisited

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Hi Bill,

I can get you several.

Earl Weltmer


----- Original Message -----
} From: "Bill Chissoe" {wchiss-at-ou.edu}
To: "MSA listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 25, 2001 3:40 PM


Thanks to all those who responded privately with good ideas about the
chiller/circulation problem, and to those who pitched in for the good of the
whole List.
I've made some further investigations into my own plumbing problem (....that
doesn't sound right....) and found out a couple additional facts.

1. The water currently in my system is pH 5.3.
2. The green crud all over my water filter was, indeed, copper.

So, I am having some corrosion problems, presumably because of the acid
water. As somebody said, iron will be attacked first where it's available,
but I found only very small traces of Fe on the filter. Perhaps there's very
little iron/steel available in my particular system.
Checking the acidity of fresh RO water from our source showed its pH to be
5.8-6.0, which is probably good enough. I guess I'll just institute a policy
of monitoring and changing the water in the system on some kind of regular
basis (semiannually?). Found no trace of biologicals on the filter so at
least that doesn't seem to be an issue.
Since it took at least five years (maybe 6 or 7) for the lines in the
ESEM to become occluded the first time, I'll assume a modest amount of care
and attention will prevent further occurrences.
It could well be that much of this copper is actually coming from the
cooling coils in the chiller itself - unfortunately this unit (a Neslab) has
a closed-up tank that you can't see inside - to add water there's just a
little neck and cap like a gas tank on a car. I think I prefer the old
Haskris unit we used to have. It just had a big open tank that you could
easily inspect.

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Fri Jan 26 10:38:08 2001



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Fri, 26 Jan 2001 10:07:43 -0500
Subject: Re: black & white print processors

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Re: black and white print processors:

I can recommend the MohrPro print processor. We've had it for a year and a
half and it's been great. It's dry-dry, compact, fast and fairly easy to
clean. I believe I bought it from EMS for around 4000$.

Maria

At 09:41 AM 1/25/01 -0500, Mary Gail Engle wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html


From daemon Fri Jan 26 10:39:13 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 26 Jan 2001 08:34:53 -0800
Subject: Re: FW: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
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Dear Sam,
I have a similar system and I definitly get green copper oxide flakes
accumulating in my reservoir. Some of the brass fittings in my microscope
water system have almost completely lost their inside flanges to corrosion.
I haven't seen any iron oxide residue, but I don't know all the metals in
the water system.
At 07:24 PM 1/25/01 -0600, you wrote:
} Fellow Microscopists:
} As some one on the metallurgical side of things, I may be able to
} help. Chris Jeffry is on the right track. The corrosion product from Cu/Fe
} couples. such Cu pipe and Fe fittings, may be magnetite, a black, magnetic
} residue or, maybe, red rust but not green Cu compounds. In such a couple, Fe
} corrodes and the Cu does not. I'd bet on algae as the green material. You
} biologists ought to be able to tell if it's algae.
}
} regards,
}
} Sam Purdy
} Technical Center
} National Steel Corp.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Jan 26 10:50:55 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Fri, 26 Jan 2001 10:47:23 -0600
Subject: Texas Society for Microscopy Spring Meeting announcement and call for

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**CALL FOR PAPERS**

The 2001 Spring Meeting of

THE TEXAS SOCIETY FOR MICROSCOPY
“Embracing All Forms of Microscopy”


April 5-7, 2001

Houston Marriott, Medical Center
6580 Fannin Street
Houston, TX 77030

(713) 770-8100
www.marriotthotels.com

Mention that you are with TSM when making your reservations.
RATES: $79/single, double, triple or quad
HOTEL RESERVATION DEADLINE: March 12, 2001
After this date reservations will be accepted on a space available
basis only with convention rates not guaranteed.

The meeting will open with a Thursday evening social (7:00 pm).

THURSDAY WORKSHOP
“Atomic Force Microscopy”
Coordinated by Steve Zeigler
FRIDAY GUEST SPEAKER
Cell cycle, sperm dimorphism and sperm cell biology in flowering
plants
Presented by Scott D. Russell
SATURDAY WORKSHOP
“Cryo-Microtomy”
Coordinated by Al Coritz

If you need fore information on the meeting, please contact Pam
Neill, Pamela.Neill-at-AlconLabs.com, or phone her at 817-568-6497.
(TSM members: you will receive this information by surface mail and
e-mail.)

ABSTRACTS MUST BE RECEIVED BY: March 12, 2001
Mail abstracts to: Camelia G.-A. Maier, Ph.D.
Assistant Professor
Texas Woman's University
Department of Biology, GRB 328
Denton, TX 76204
Tel.: 940-898-2358 (office)
E-mail: f_maier-at-twu.edu

If you need format documents and are not a member of TSM, please
contact Dr. Maier as listed above.
(TSM members will receive these documents by surface mail and e-mail.)







From daemon Fri Jan 26 11:16:18 2001



From: David T. Hoelzer :      hoelzerd-at-ornl.gov
Date: Fri, 26 Jan 2001 12:13:54 -0500
Subject: Re: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
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Hello,

After following this thread dealing with corrosion occurring in the cooling
system, I've decided to add my experience. In my former position at NYSCC,
I had a similar problem of corrosion products clogging the cooling system
on the ion mill, which was a closed loop system combined with a Neslab
chiller. Although there were two cast iron fittings used (not including
the chiller unit), the tubing and most of the fittings were Cu or brass.
However, the corrosion products were definitely Cu-based. Why? I examined
at times the elbow and tube size reduction fittings and determined that
these were mostly the problem. I surmised that both cause turbulence in
the water flow which promotes erosion corrosion. This would especially be
more of a problem in regions of the tubing and fittings that became hot,
such as near the bottom of the diffusion pump heater as well as on the
trailing side of the flow direction from the diffusion pump. I learned to
first check the elbow fitting on the trailing side of the pump since it
almost always got clogged by corrosion products. Thus, be aware of these
transitions points in the water flow path and on the proximity to the heat
load as potential areas for this problem.

David
* * * * * * * * * * * * * * *
David T. Hoelzer, Ph.D.
Metals and Ceramics Division
Oak Ridge National Laboratory
Bldg. 4500S, Mail Stop 6151
P. O. Box 2008
Oak Ridge, Tennessee 37830
* (865) 574-5096 {Work}
* (865) 574-0641 {Fax}


From daemon Fri Jan 26 11:56:01 2001



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Fri, 26 Jan 2001 12:50:57 -0500
Subject: Re: Fun with vacuum systems

Contents Retrieved from Microscopy Listserver Archives
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Hi

I found an interesting page at:

http://www.moenv.go.kr/english/tit12/eco49.htm

on Demetalization (the selective corrosion caused by the difference
in ionization tendencies among the components of alloy). Maybe that
explains why the copper pieces are floating around ... would be
interested in more details though,

Best regards,

Edgar



--


________________
Dr. Edgar Voelkl
Senior Development Staff Member
ORNL
Bldg 4515, MS 6064
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov


From daemon Fri Jan 26 12:04:22 2001



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Fri, 26 Jan 2001 10:17:54 -0800 (PST)
Subject: IHC classes in N. California?

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Yes, you can do this with Low-temperature SEM. I wrote a paper on this
in 1989
vanGardingen, PR, Jeffree, CE and Grace, J (1989) Variation in
stomatal aperture in leaves of Avena fatua L. observed by
low-temperature scanning electron microscopy. Plant Cell and
Environment 12, 887-897.

I have to say that the method is not applicable to all species - just
those which offer an unobstructed view of the aperture.
If you need any more info on this just ask.
Chris

----- Original Message -----
} From: "Beth Richardson" {beth-at-dogwood.botany.uga.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, January 25, 2001 6:42 PM


Dear List folks

I have a colleague who would like to take some workshops or classes in
Immunohistochemistry-Microscopy techniques. She's just a beginner, so
something that starts with the basics would be ideal. Does anyone have
recommendations on offerings of either in the greater bay area to greater
Sacramento area? I can think of a few local schools that may teach such
courses, but I don't know about their policies about non-students taking
such classes, especially when it involves hands-on labwork.

Thanks,
Pauline Yu
Microscopist Technician
USDA-ARS-WRRC
splene-at-pw.usda.gov



From daemon Fri Jan 26 14:24:38 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 26 Jan 01 12:28:54 -0800
Subject: RE: Immuno gold

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 26 Jan 01 12:28:54 -0800
Subject: RE: Immuno gold

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Reply to: RE: Immuno gold
Although I didn't really understand the problem with your antibody (rabbit and goat-gold, how could it react with the sample?), there may be a solution to not using rabbit antibodies.

If you purchase an unconjugated goat antibody that binds protein A, you could then use the protein A to visualize antibody binding. Examples of antibodies that bind protein A include those from pig, dog, guinea pig, rat IgG2c and IgG1, and any IgG2a, IgG2b immunoglobulins from mouse. I think human antibodies are also good for binding protein A but have not yet found any volunteers willing to be subjected to the repeated injections required.

Paul Webster.



Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



Tracey M. Pepper wrote:
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} I have a researcher who needs an unusual type of gold-conj. secondary } antibody. Her primary is made in goat and the only known available } anti-goat is made in rabbit. Her tissue has cross-reactivity with } rabbit. Is there any other species (donkey, dog, Brazilian opossum...) } that anyone knows of as an anti-goat gold conjugate secondary? Do we have } to make a home-made version?
} Thanks!
}
} Tracey Pepper
} Supervisor
} Bessey Microscopy Facility
} Iowa State University
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} fax: 515.294.1337
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From daemon Fri Jan 26 14:36:58 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Fri, 26 Jan 2001 12:33:19 -0800
Subject: RE: black & white print processors

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We still use a lot of film too, but gave up on darkroom printing in favor of
scanning and digital image processing some time ago. Film recording still
offers significant advantages over the in-microscope CCD cameras for certain
applications (we use both). Compared to darkroom printing, the scanning/digital
processing/dye-sub printing route is a big cost saver and reduces generation of
chemical wastes. Also, digital processing with programs like PhotoShop or
CorelPaint is much faster and gives better control of the process. All the time
spent and print paper wasted dodging and getting tones right certainly won't be
missed. Also, digital images avoid the extra step of dealing with prints in
presentations and publications.

Larry

Larry Thomas
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto: Larry.Thomas-at-pnl.gov


----------
From: Maria Ericsson
Sent: Friday, January 26, 2001 7:07 AM
To: Mary Gail Engle; Microscopy-at-sparc5.microscopy.com
Subject: Re: black & white print processors

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Re: black and white print processors:

I can recommend the MohrPro print processor. We've had it for a year and
a
half and it's been great. It's dry-dry, compact, fast and fairly easy to

clean. I believe I bought it from EMS for around 4000$.

Maria

At 09:41 AM 1/25/01 -0500, Mary Gail Engle wrote:

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} Dear listers,
} Our 10 year old Ilford print processor is breaking down piece by
} piece. We've about reached the point of diminishing returns and so are

} considering replacing it. Not everyone has switched to digital so we
} use quite a lot of film. Can any of you recommend a good black and
white
} print processor?
}
} Thank you,
}
} Mary Gail Engle
} Manager
} Electron Microscopy & Imaging Facility
} University of Kentucky

____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html



From daemon Fri Jan 26 15:34:20 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Fri, 26 Jan 2001 15:33:26 -0600
Subject: 3mm glass,tem

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Hi Ya'll,
if there is a kind sole out there with a tem sample prep hole saw, that
is willing into cut ~20 TEM disk from microscope slide covers, I am
willing to pay for the service.
Please contact me off line.

Bruce Brinson
Rice U.



From daemon Sat Jan 27 09:32:25 2001



From: m.delcerro-at-worldnet.att.net ()
Date: Sat, 27 Jan 2001 09:18:24 -0600
Subject: Ask-A-Microscopist: LM/EM of mosses

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor
---------------------------------------------------------------------------

Email: m.delcerro-at-worldnet.att.net
Name: Manuel del Cerro
State: New York


Question: I would very much like to see light and electron microscopy
(TEM/SEM) of leaves of mosses. Could you please give me the name of a book
or web site that will have these?
Thanks!

---------------------------------------------------------------------------




From daemon Sat Jan 27 09:44:45 2001



From: Stefan.Geimer :      stefan.geimer-at-uni-koeln.de
Date: Sat, 27 Jan 2001 16:40:42 +0100
Subject: embedding (human) spermatozoa for TEM

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I´am looking for a reference giving a protocol how to embedd (human)
spermatozoa for TEM. What I need also to know is if and how I have to
isolate the spermatozoa out of the ejaculate. It would be advantageous
for me to have a relatively dense pellet of spermatozoa and I have no
idea how much percent of the ejaculate are spermatozoa and how much is
`junk`.

Tanks

Stefan


****************************
Dr. Stefan Geimer
University of Cologne
Botanical Institute
Gyrhofstr. 15
D-50931 Cologne
Germany

phone:
office: +49-(0)221-470-7022
lab: +49-(0)221-470-3795

fax: +49-(0)221-470-5181
****************************




From daemon Sat Jan 27 20:48:13 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Sat, 27 Jan 2001 21:28:12 -0500
Subject: RE: 3mm glass,tem ----I got it covered.

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I have a stash of about 50 cut from coverslips that I don't need. I don't know what thickness they are, but they are probably #1 or #2. Send me your address.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Bruce Brinson [mailto:brinson-at-rice.edu]
Sent: Friday, January 26, 2001 4:33 PM
To: MSA Listserver
Subject: 3mm glass,tem


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Hi Ya'll,
if there is a kind sole out there with a tem sample prep hole saw, that
is willing into cut ~20 TEM disk from microscope slide covers, I am
willing to pay for the service.
Please contact me off line.

Bruce Brinson
Rice U.




From daemon Sat Jan 27 20:48:13 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Sat, 27 Jan 2001 21:40:41 -0500
Subject: RE: Fun with vacuum systems

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Careful.
Galvanic corrosion is dependent on the relatvie electronegativities of the alloys involved in the corrosion cell. Not all Fe alloys will corrode in contact with copper. I know for instance, that 304SS is about the same as copper, but a little more noble and therefore it is the copper that will corrode.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Purdy, Sam [mailto:SPurdy-at-nationalsteel.com]
Sent: Thursday, January 25, 2001 8:24 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: FW: Fun with vacuum systems


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Fellow Microscopists:
As some one on the metallurgical side of things, I may
be able to
help. Chris Jeffry is on the right track. The corrosion
product from Cu/Fe
couples. such Cu pipe and Fe fittings, may be magnetite, a
black, magnetic
residue or, maybe, red rust but not green Cu compounds. In
such a couple, Fe
corrodes and the Cu does not. I'd bet on algae as the green
material. You
biologists ought to be able to tell if it's algae.

regards,

Sam Purdy
Technical Center
National Steel Corp.

} ----------
} From: Chris Jeffree
} Sent: Thursday, January 2001, 4:49 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Fun with vacuum systems
}
}
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}
}
} Maybe US plumbers are better trained, but UK heating systems are
} almost always iron (pressed steel steel) radiators with copper
} connecting pipes. Other metals involved are brass valves and
} connectors, and lead/tin solder at soldered joints. Systems like that
} corrode unless inhibitors are used. The sludge that collects in my
} copper/iron central heating system is deep black, like
indian ink, not
} green.
} Is it possible that your green sludge is not copper but algae??
} Transparent plastic tubing in the system may allow alagal growth,
} provided there is some source of carbon. Bacterial/fungal
} decomposition of glycol could provide that.
} Chris
}
} ----- Original Message -----
} } From: "John J. Bozzola" {bozzola-at-siu.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, January 24, 2001 9:03 PM
} Subject: Re: Fun with vacuum systems
}
}
} }
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} }
--------------------------------------------------------------------
} ---.
} }
} }
} } Hey, deja-vu all over again......
} }
} } I encountered a similar (green sludge and corrosion) problem on the
} } DP's and recirculating water system of our TEM several years ago. I
} } assumed that it was a reaction between the iron and copper tubing
} } used in the system. Old time plumbers, for example, always warned
} } about directly connecting iron and copper tubing because of the
} } corrosion that would occur. I KNOW that on our DP's the iron
} fittings
} } have copper connections. Oh well....
} }
} } JB
} }
} } --
} } ##############################################################
} } John J. Bozzola, Ph.D., Director
} } I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} } 750 Communications Drive - MC 4402
} } Southern Illinois University
} } Carbondale, IL 62901 U.S.A.
} } Phone: 618-453-3730
} } Fax: 618-453-2665
} } Email: bozzola-at-siu.edu
} } Web: http://www.siu.edu/departments/shops/cem.html
} } ##############################################################
} }
}
}





From daemon Sun Jan 28 11:12:24 2001



From: Paula Allan-Wojtas :      ALLANWOJTASP-at-em.agr.ca
Date: Sun, 28 Jan 2001 11:54:08 -0500
Subject: What is Food Structure and Functionality?

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Dear all,

I'd like to thank Tim Richardson for his comments and interest in our upcoming Food Structure and Functionality Symposium in May 2001, which was recently publicized on this list. I wanted to take advantage of this opportunity to explain a little about this fascinating area of study.

Food Structure and Functionality is the epitome of applied microscopy and imaging. It is not merely imaging but the integrating of this information with other food quality attributes especially texture. One of our fundamental beliefs is that every change in the behaviour of a food is accompanied by a structural change at a heirarchy of levels - from the macroscopic to the molecular. In other words, this is a structure/function relationship.

If you think about this a little, you can see how the quality of food can be assessed and controlled using these methods. Processed foods with certain properties can be designed by following the evolution of structure using these methods. Food Structure and Functionality covers all aspects of foods, from their plant and animal origins, from breeding, growth and management, harvest and post-harvest (plants), and finally to the processing stage.

At present, Food Structure and Functionality researchers are scattered all over the world. There are many labs which do this type of work and don't use this name. I know that there are a number of labs in Canada's Federal Department of Agriculture (Agriculture and Agri-Food Canada) which do this type of work, but there is only 1 in our department, at our Research Centre in Kentville, Nova Scotia (1 hour drive from Halifax/Dartmouth) which actually calls itself a Food Structure lab (our lab). USDA has many labs which do this research. Many of the large food companies in the world routinely use these techniques as a valuable tool - some are able to talk about what they do while others cannot. In Europe, labs using these techniques are numerous and are well supported.

Those of us who come to this field of study are either classically trained microscopists from the bio/medical sciences, or food scientists/technologists who have discovered the power of imaging techniques in their daily work. To my knowledge, there is no university which specifically offers courses in Food Structure and Functionality, but it is the basis of many challenging PhD theses.

Hopefully this explains a little about us for the curious. You can find out more about our group at the AOCS website (www.aocs.org, on the the Food Structure and Functionality Division webpage). Also, Milos Kalab, a pioneer in Food Structure research has a website called Food under the Microscope which is really worthwhile to visit at:

http://www.magma.ca/~scimat

Thanks for the opportunity of telling you all of this.

Paula.





From daemon Sun Jan 28 12:52:56 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Sun, 28 Jan 2001 12:16:59 -0600
Subject: thx,3mm glass, tem

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Hi Yall,
I tried to send this with "thanks, 3mm glass, tem" in the subject
line...guess ''thanks" got cought in the spam filter.
} Wow! My thanks to the MSA list server & those of you that reponed. My
need
} for 3mm disk has been met faster than I got a copy of my own messsage
{:o{)}

Bruce Brinson
Rice U.
}



From daemon Mon Jan 29 03:59:22 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Mon, 29 Jan 2001 05:41:26 -0500
Subject: Re: teaching microscopy CD-ROMS

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Sorry for not getting around to this before now.

First, I am neither an expert in physics or mathematics. Unfortunately, I
shot from the hip and included my own personal terminology. My
understanding of the world is based in what I would call 'intuitive
modeling'. In terms of modern physics, the electron does have a definable
mass. However, with my aversion to the mathematics of quantum physics, I
struggle to find a more intuitive understanding. The fitting of
statistical descriptions to empirical data holds far less interest to me
than the discovery of theoretical considerations that explain empirical
data.

In doing so, I consider the electron as having no gravitational mass, but
rather a mass defined at this point based solely on its energy. This I
base on the simple minded concept of classical physics of particles as
'billiard balls'. In that concept, two features are required for 'mass'-
spatial dimension and gravitational effects. At this point in time, I am
unaware of any experiments that can demonstrate that either property can be
attributed to the electron.

Instead, I consider the electron as a dimensionless bundle of energy that
affects matter solely through contactless energy interactions. While
Einstein managed to equate energy with matter, the matter portion of his
equations gained considerable weight from the c-squared portion. That
should have shown up by now in the case of the electron.

In regards to the matter at hand (no pun intended), it can not be denied
that the accelerated electron carries a field. As even relativistically
accelerated electrons have, of yet, not been shown to have an increased
gravitational or spatial mass, I assume, probably incorrectly, that the
apparent increase in mass is due to an increase in their energy. At least
a portion of that increase should be in their electro-magnetic effects, and
thus their field strength and their effect on nearby charges. Consider
quantum explanations of the Bremstralung radiation, which is dependent on
the acceleration of the electron yet relies on the action at a distance of
a field effect.

On the level of flux densities involved in electron microscopes, I also
assume that the field densities brought on the microscopic level by
accelerated and focussed electron beams can easily approximate those on a
macro level by an electrostatically charged body brought within millimeters
of a surface. The matter of scale in a focussed electron beam can not be
underestimated.

While Einstein was clearly successful in uniting our concepts of energy and
mass, he also left clear distinctions. If one assumes that there is a
gravitational mass associated with the electron, then one would have to
assume an infinite gravitational field gradient if the electron is truly
dimensionless. Such a gradient would essentially extend beyond the
Swartzchild radius and make the electron a small 'black hole' - essentially
undefinable in our current understanding. I honestly have not done the
math myself, but I think that the difference in energy attributable to the
electron's charge and its mass is sufficient that modern experiments should
have been able to detect them.

That mass effect should become apparent when the particle is brought near
the speed of light, as the gravitational mass would then be greatly
amplified. But no such effect has been measured, as far as I know.

Frankly, I think we have a long way to go to explain the electron. In the
mean time, I reserve the right to define my own understanding of it,
although I may regret making those views public.

On Wednesday, January 24, 2001 9:11 AM, Mike Bode
[SMTP:mb-at-Soft-Imaging.com] wrote:
} { { File: ATT00005.txt; charset = windows-1252 } }

Allen,

I am not sure I am following you. You say

"The best high energy experiments have been unable to assign a 'mass' to
the
electron."

To my knowledge and from a list of "fundamental constants" in my "Solid
State Physics" book by Ashcroft and Mermin, the rest mass of an electron is
9.1095 x 10^(-31) kg. Just multiply that wit 1/2 v*v and you have the
kinetic Energy of the electron. Of course you have to take into account
relativistic effects. Or just multiply it with v and you have the momentum.

I think, you mistake the mass of the electron with that of the neutrino,
which indeed they have not been able to determine, although there are
indications from recent high energy physics measurements. But that's
irrelevant here.

-----Original Message-----
} From: Allen R. Sampson [mailto:ars-at-sem.com]
Sent: Monday, January 22, 2001 4:21 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


Hi

Am I permitted to say that we at Protrain do a range of training CD for
SEM, TEM and EDX? Ready in a week or two will be a CD on Monitoring &
Maintaining an Electron Microscope that will tidy up a number of the MSA
mailings on SEM and TEM performance as well as instrument calibration.

As an ex service engineer, and now with our training courses, I find the
magnification "problems" mentioned by your mailings to be absolutely
NORMAL. If you have checked as many instruments as I have you will have
seen all the "errors" mentioned.

Surely the important point in the discussions is not where the
magnification is, high or low, but how consistant it is? For example if you
have an old instrument that was calibrated by the manufacturer just the
differences in glass plate to film thickness must make a difference but
does it matter once you know? Specimen position, therefore objective lens
current, is always very critical as is the high voltage level. If you
monitor high voltage over a period of several hours you will detect a
change so during the working day I know that instruments do change in
relation to their calibration.

Try this experiment? When you switch on the high voltage in the morning
insert a test specimen (holey carbon film) find true focus (no fringe) and
immediately take a test picture at say 50,000kX. Leave the microscope on
and take further pictures every hour BUT most important count the focus
clicks (their value will be found in your instruction book), that is the
correction that you make from one test picture to the next. After a few
hours one would expect the high voltage to be stable and very little focus
change to take place.

Now insert a grating replica and take a picture at the exact same focus you
had for your last test picture. Now turn the focus back to the position
that you had for your first test picture and adjust the eucentric Z to
bring the sample back in focus. Measure the two pictures and see how your
microscope performs. Plus or minus 5% is very good, but what answers will
you get?

If you have a gas filled high voltage tank the system will settle far
quicker than an oil filled tank. You are waiting for heat gained by the
tank to equal the heat lost; then the system will become stable!

Have fun

Steve Chapman
Senior Consultant Protrain
For consultancy and training by professionals World Wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com


From daemon Mon Jan 29 09:00:50 2001



From: Brian Wajdyk :      Brian.Wajdyk-at-onsemi.com
Date: Mon, 29 Jan 2001 08:03:23 -0700
Subject: SEM and LM image capture on PC

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Fellow Microscopists,

I would like suggestions for any frame grabber cards, for image capture,
for both light microscope (S-video) and SEM (NTSC) applications. All of
our microscopes are connected to PC based Windows NT systems. We have
tried several cards with varying success. I am hoping that some of you
have found cards that you are happy with. Any suggestions would be
appreciated.

Regards,

Brian Wajdyk
Senior Electron Microscopist
On Semiconductor
Product Analysis Laboratory
Brian.Wajdyk-at-onsemi.com


From daemon Mon Jan 29 09:15:27 2001



From: Jennifer Palmer :      jpalmer-at-cvmbs.colostate.edu
Date: Mon, 29 Jan 2001 08:11:29 -0700 (Mountain Standard Time)
Subject: Re: embedding (human) spermatozoa for TEM

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On Sat, 27 Jan 2001 16:40:42 +0100 "Stefan.Geimer"
{stefan.geimer-at-uni-koeln.de} wrote:

} I´am looking for a reference giving a protocol how to embedd (human)
} spermatozoa for TEM. What I need also to know is if and how I have to
} isolate the spermatozoa out of the ejaculate. It would be advantageous
} for me to have a relatively dense pellet of spermatozoa and I have no
} idea how much percent of the ejaculate are spermatozoa and how much is
} `junk`.
}
} Tanks
}
} Stefan
}
}
} ****************************
} Dr. Stefan Geimer
} University of Cologne
} Botanical Institute
} Gyrhofstr. 15
} D-50931 Cologne
} Germany
}
} phone:
} office: +49-(0)221-470-7022
} lab: +49-(0)221-470-3795
}
} fax: +49-(0)221-470-5181
} ****************************
} Hope this helps.
Our lab routinely embeds rabbit and horse spermatozoa. You really
will need to centrifuge the ejaculate into a reasonably dense pellet.
I wouldn't worry about excess "junk".
We fix the ejaculate in 4% glutaraldehyde, 5% sucrose in 1M
cacodylate for 24hr. Wash with buffer.
Osmicate in 1% OsO4 for 1.5hr. Wash in buffer, centrifuge then
embed in 2% low melting point agarose(Sigma A5030), 2% sucrose in
buffer. This does very well in 1cc syringes that have been cut off at
both ends and the plunger cut even with the bottom so that you can
fill them to about 0.5cc and they will fit in a centrifuge tube. You
can warm and mix the sperm and agarose in the syringe, keep warm and
centrifuge, then push out a perfect pellet. We then use a standard
dehydration and epon protocol for TEM.
Sorry I don't have published reference for this. I have it as a
hand-me down protocol.
Jennifer
}
}

====================================
Jennifer Palmer
ARBL
Colorado State Univ
Ft. Collins, CO 80523-1683 , USA
voice:(970)491-1770
fax:(970)491-3557

jpalmer-at-cvmbs.colostate.edu
====================================



From daemon Mon Jan 29 10:11:08 2001



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Mon, 29 Jan 2001 10:57:45 -0500
Subject: Cartoon Depictions of EM

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To All,
I am hoping someone can help me locate, i.e. send me a copy of, a Gary
Larson Far Side cartoon which I saw several years ago. I would like to
include this cartoon in an upcoming presentation I will be giving at a local
university.

The cartoon showed a janitor who had entered an electron microscope lab,
propped his mop up against the wall and was shown peering into the
microscope to glimpse a view of an apple he had placed in the 'viewing'
area. I hope this description sounds familiar to someone. Can anyone help?
Although it is not my intention, this note may well start an exchange of all
cartoons illustrating the amusing side of work in the microscopy lab or any
lab for that matter.

So, can one of you Larson fans help me out? Thanks.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Mon Jan 29 10:34:19 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 29 Jan 2001 09:43:55 -0600
Subject: Re: SEM and LM image capture on PC

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In what way were you not satisfied with the boards you have already tried?

Resolution - NTSC is only good for about 640 pixels across. That is too low
for my use. I prefer 1024. Many prefer even higher numbers. The NTSC signal
just doesn't have it there to be captured.

Noise - I would think most NTSC signals would be noisy and that would show
up in a single frame capture. You should be able to do some frame averaging
or integration to improve that. That depends on the system.

Usability - This will be a function of software. Because of the resolution
issue, I don't even have such a system anymore. Well maybe I do, sort of.
We still have a couple of units from Dazzle that we use very infrequently.
When we do use them, it is for the purpose of recording a video.

At 08:03 AM 1/29/2001 -0700, you wrote:

} Fellow Microscopists,
}
} I would like suggestions for any frame grabber cards, for image capture,
} for both light microscope (S-video) and SEM (NTSC) applications. All of
} our microscopes are connected to PC based Windows NT systems. We have
} tried several cards with varying success. I am hoping that some of you
} have found cards that you are happy with. Any suggestions would be
} appreciated.

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Jan 29 13:37:32 2001



From: Katja M. Wolski :      kwolski-at-hsc.usf.edu
Date: Mon, 29 Jan 2001 14:21:02 -0500
Subject: Re: embedding (human) spermatozoa for TEM

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{!doctype html public "-//w3c//dtd html 4.0 transitional//en"}
{html}
{font size=-1} Here are some references you might find useful for isolate
and embed spermatazoa. {/font}
{p}
{hr WIDTH="100%"}
{br}  
{p} Aleman V. Trejo R. Morales E. Hernandez-Jauregui P. Delhumeau-Ongay
G.  A simple and rapid technique to
{br} isolate enriched populations of spermatocytes and spermatids from the
immature rat testis.  {b} {i} Journal of Reproduction & Fertility {/i} . {/b}

{i} 54(1):67-75, 1978 Sep. {/i}
{p} Baccetti B. Burrini AG.  An improved method for the scanning electron
microscopy of spermatozoa.  {b} {i} Journal of {/i} {/b}
{br} {b} {i} Microscopy {/i} . {/b}   {i} 99(1):101-7, 1973 Sep. {/i}
{p} Basrur PK. Ackerley CA. Reyes ER. Doig PA.  Surface morphology
of the spermatozoa in infertile Welsh ponies.  {b} {i} Scanning Electron
Microscopy {/i} . {/b}   {i} (3):511-6, 1979. {/i}
{br}  
{p}
{hr WIDTH="100%"}
{br}  
{br}  
{p} {font size=-1} -- {/font}
{br} {font size=-1} Katja M. Wolski {/font}
{br} {font size=-1} Ph.D. Student {/font}
{br} {font size=-1} University of South Florida College of Medicine {/font}
{br} {font size=-1} Department of Anatomy {/font}
{br} {font size=-1} Andrology Laboratory {/font}
{br} {font size=-1} 12901 Bruce B. Downs Blvd., MDC 6 {/font}
{br} {font size=-1} Tampa, FL 33612-4799 {/font}
{br} {font size=-1} Office:  813.974.6102 {/font}
{br} {font size=-1} Lab:  813.974.9434 {/font}
{br} {font size=-1} Fax:  813.974.2058 {/font}
{br} {font size=-1} E-mail:  kwolski-at-hsc.usf.edu {/font} {/html}



From daemon Mon Jan 29 13:44:51 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Mon, 29 Jan 2001 12:39:25 -0700 (MST)
Subject: Image Pro wrap-up

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If anyone is interested, the general response to my question about Image
Pro software(s) was that the package is nice, easy to learn and use (big
plus!), and reasonably priced. There is a demo version available from
Media Cybernetics (http://www.mediacy.com/). The "Express" package is
pretty basic, but can collect images, do some "rudimentary"
processing/measuring, and store. "Plus" is more advanced in its analysis
capabilities, and "Pro" is the suped-up version.

I also heard that the Russ plug-in for Adobe Photoshop is similar to Image
Pro; I've heard wonderful things about it, but haven't had a chance to
play with it, yet. And I don't know how close it is to the Media Cyb.
stuff.

Thanks again to everyone who responded!

Tamara


|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|





From daemon Mon Jan 29 14:31:28 2001



From: DrJohnRuss-at-aol.com
Date: Mon, 29 Jan 2001 15:41:41 EST
Subject: Re: Image Pro wrap-up

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With all due respect (long pause): Isn't this thread getting a bit old?

The person who asked this question has long since had her question answered.

Doesn't this remind one of two dogs and only one tree?

Thank You,

Earl


----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Mike Bode'" {mb-at-Soft-Imaging.com} ; "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 29, 2001 1:55 AM


I've just faxed it to you. I enlarged it first so shrinking it should make
the fax look better. If it doesn't look OK, I can either scan and e-mail or
snail mail it to you.

Ron L
----- Original Message -----
} From: Gerroir, Paul J {Paul.Gerroir-at-crt.xerox.com}
To: Microscopy-at-MSA. Microscopy. com (E-mail)
{Microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 29, 2001 10:57 AM



In a message dated 1/29/01 3:55:20 PM, thoward-at-UNM.EDU writes:

} I also heard that the Russ plug-in for Adobe Photoshop is similar to Image
} Pro; I've heard wonderful things about it, but haven't had a chance to
} play with it, yet. And I don't know how close it is to the Media Cyb.
} stuff.

I wouldn't say they are "similar" and in fact many people use the tool kit or
fovea pro plug-ins with Image Pro Plus (in which they work just as well as in
Photoshop). The plug-ins implement a very wide range of algorithms for
processing and measurement, which no single commercial program provides.
IPPlus is quite strong in image measurement and some areas of image
processing, but has areas such as FFT processing and morphological processing
of binary images in which it is less complete. The tool kit fills in those
gaps nicely. On the other hand, the plug-ins when used with Photoshop provide
only a very limited amount of automation. You can create actions that provide
efficient batch processing of images, but you cannot automate stage control,
acquisition, and report creation as you can with an IP+ Macro. I don't see
them as competitive products, and I don't think Media Cybernetics does
either. They address different needs and in some respects are complementary.
To go beyond what I've tried to say here probably verges into the area of
commercialism that would be inappropriate for this forum, but I did think it
important to put my 2 cents worth in since the specific question was raised.

John C. Russ



From daemon Mon Jan 29 15:15:40 2001



From: Susanne Stemmer :      stemmer-at-rice.edu
Date: Mon, 29 Jan 2001 15:10:39 -0600
Subject: Postdoctoral Position in TEM (resent 1/29/01)

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Postdoctoral Position in Transmission Electron Microscopy - Rice University

Rice University, Department of Mechanical Engineering and Materials Science,
located in Houston, TX, is seeking candidates for a postdoctoral position in
transmission electron microscopy of multicomponent oxide thin films.
Applicants should have extensive and demonstrated experience in several areas
of TEM and a strong background and interest in materials problem solving.
Preference will be given to candidates with experience in high-resolution
imaging and electron energy-loss spectroscopy as well as conventional
diffraction contrast imaging.
Facilities at Rice include a JEOL 2010, field-emission SEM and high-resolution
X-ray diffractometers, as well as sample preparation. The
project will be carried out in close collaboration with the University of
Houston using a state-of-the-art field-emission TEM (JEOL 2010F), with annular
dark-field detector, EDS and EELS capabilities. The position is available
immediately. Duration about 1-2 years, salary is commensurate with
qualifications. Candidates with a Ph.D. in Materials Science or Physics will
be given preferred consideration.
Interested candidates should send a curriculum vitae, publication list and the
names and email addresses of at least three references to:

Prof. Susanne Stemmer
Rice University
Department of Mechanical Engineering and Materials Science
MS 321
6100 Main Street
Houston, TX 77005-1892
stemmer-at-rice.edu

Applicants must have proof of legal authorization to work in the United
States.
Rice University is an Affirmative Action/Equal Opportunity Employer.


From daemon Mon Jan 29 17:45:48 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 29 Jan 2001 17:26:28 -0500
Subject: thanks - stomata replies

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Hi,
I just want to thank all the people who wrote to me about SEM of stomata. I
regret that I do not have the time to reply individually.
I appreciate all the advice and references. I will try both chemical and
cryo SEM (just as soon as our central facility gets a cold stage).
This is such a great list!
thanks again,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Mon Jan 29 17:47:51 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 29 Jan 2001 15:11:55 -0800 (PST)
Subject: Batson's No 17 Plastic Replica and Corrosion Kit

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Hello,
I have someone wanting to do some corrosion casts from parts of a rat. I
was wondering if anyone has any experience with Batson's No 17 Plastic
Replica and Corrosion Kit. I believe it is a methyl methacrylate monomer.

We had trouble in the past in working with resins in the SEM. They tended
to melt under the beam, even after sufficient coating. So I was hoping,
to get any comments about this or other resins.
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Mon Jan 29 20:25:51 2001



From: Eric :      biology-at-ucla.edu
Date: Mon, 29 Jan 2001 18:19:16 -0800
Subject: Question about NIH Image

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To my Fellow Microscopists,

First of all thanks for all the info on cleaning the LKB Knife boats
we are using here and I will try and get them to switch to dental
wax.

Secondly, I need to know where I can find NIH Image if it is still
available and is it availabe for the PC now??

Thanks

Eric A. Rosen
UCLA Medical Center
Electron Microscopy Lab


{ { {This message is made of 100% recycled electrons} } }
|"|
-`^'- {_*_} ` _ , ' _|_|_
(o o) (o o) - (o)o) - (o o)
ooO--(_)--Ooo--8--(_)--Ooo-ooO'(_)--Ooo-ooO--(_)--Ooo
**Everyone has a photographic memory. Some just don't have film!
Eric {Los Angeles, California}
Go Check out my new and alomst finished webpage at:
http://www.geocities.com/Yosemite/Rapids/4855/
_ /| \\\|///
\'o.O' ( o o )
==========oOO==(_._)==OOo==============oOO==(_)==OOo========



From daemon Mon Jan 29 20:37:19 2001



From: Frederick Schamber :      fhscham-at-stargate.net
Date: Mon, 29 Jan 2001 21:44:31 -0500
Subject: Re: Question

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Allen:

Since you are admittedly creating your own interpretation of realitiy in these
postings, it is a bit hard to understand when you depart from accepted physics
whether you are doing that by intent or otherwise, but a few comments do seem
to be in order:

(1) First of all, there is absolutely no need to drag either quantum mechanics
or relativity into the discussion. The discussion of an electron microscope
can be accomplished very nicely by assuming that an electron is a classical
particle -- the only exception is when we deal with diffraction of the electron
by a small aperture or other feature. We also do not generally need to invoke
quantum mechanics to discuss the interaction of free electrons with matter
through electromagnetic fields -- this is due to the fact that the
electromagnetic forces are very long range and it matters not a bit whether the
electron is a point or an extended distribution. Unless you are operating a
VERY high energy electron microscope, relativistic effects are also
inconsequential. In short, a late nineteenth century physicist would not have
had any trouble understanding how an electron microscope operates (except for
the aforementioned matter of diffraction) nor would he have had any problem
with understanding how the field of the incident electron interacted with
conduction electrons in the specimen (ionization would be a different story due
to the atomic shells involved). So it seems to me that you are struggling to
create a great deal of complexity where none needs to exist.

(2) Your remarks about the ambiguity of the mass of the electron are
"remarkable". Does an electron have mass? Of course! The rest mass of an
electron is well-known (9.1x10^-31kg) and confirmed in many experiments. One
of the most direct ways of observing it is via the positron "annihilation"
gamma rays emitted in many nuclear decay schemes. The mechanism is as
follows: a positron (anti-matter version of the electron with a positive
charge) is emitted in the nuclear decay. The positron quickly encounters a
normal electron and they annihilate in a flash of pure energy, giving off a
pair of oppositely directed gamma rays (as required by the conservation of
momentum), each gamma ray carrying 511,000 eV energy, which, using E=mc^2, is
just the rest mass of the electron! But you don't have to get this exotic to
know that electrons have mass -- there are countless devices (such as scanning
electron microscopes and TV picture tubes) which routinely manipulate their
trajectories -- and they obey F=ma with the aforesaid mass. Since the behavior
of electrons in the classical, quantum mechanical, and relativistic domains are
all beautifully predicted by their representation as having mass (and I
personally know of no experiment or theory to the contrary), why on earth would
one want to invent an alternate theory?

Fred Schamber


"Allen R. Sampson" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Sorry for not getting around to this before now.
}
} First, I am neither an expert in physics or mathematics. Unfortunately, I
} shot from the hip and included my own personal terminology. My
} understanding of the world is based in what I would call 'intuitive
} modeling'. In terms of modern physics, the electron does have a definable
} mass. However, with my aversion to the mathematics of quantum physics, I
} struggle to find a more intuitive understanding. The fitting of
} statistical descriptions to empirical data holds far less interest to me
} than the discovery of theoretical considerations that explain empirical
} data.
}
} In doing so, I consider the electron as having no gravitational mass, but
} rather a mass defined at this point based solely on its energy. This I
} base on the simple minded concept of classical physics of particles as
} 'billiard balls'. In that concept, two features are required for 'mass'-
} spatial dimension and gravitational effects. At this point in time, I am
} unaware of any experiments that can demonstrate that either property can be
} attributed to the electron.
}
} Instead, I consider the electron as a dimensionless bundle of energy that
} affects matter solely through contactless energy interactions. While
} Einstein managed to equate energy with matter, the matter portion of his
} equations gained considerable weight from the c-squared portion. That
} should have shown up by now in the case of the electron.
}
} In regards to the matter at hand (no pun intended), it can not be denied
} that the accelerated electron carries a field. As even relativistically
} accelerated electrons have, of yet, not been shown to have an increased
} gravitational or spatial mass, I assume, probably incorrectly, that the
} apparent increase in mass is due to an increase in their energy. At least
} a portion of that increase should be in their electro-magnetic effects, and
} thus their field strength and their effect on nearby charges. Consider
} quantum explanations of the Bremstralung radiation, which is dependent on
} the acceleration of the electron yet relies on the action at a distance of
} a field effect.
}
} On the level of flux densities involved in electron microscopes, I also
} assume that the field densities brought on the microscopic level by
} accelerated and focussed electron beams can easily approximate those on a
} macro level by an electrostatically charged body brought within millimeters
} of a surface. The matter of scale in a focussed electron beam can not be
} underestimated.
}
} While Einstein was clearly successful in uniting our concepts of energy and
} mass, he also left clear distinctions. If one assumes that there is a
} gravitational mass associated with the electron, then one would have to
} assume an infinite gravitational field gradient if the electron is truly
} dimensionless. Such a gradient would essentially extend beyond the
} Swartzchild radius and make the electron a small 'black hole' - essentially
} undefinable in our current understanding. I honestly have not done the
} math myself, but I think that the difference in energy attributable to the
} electron's charge and its mass is sufficient that modern experiments should
} have been able to detect them.
}
} That mass effect should become apparent when the particle is brought near
} the speed of light, as the gravitational mass would then be greatly
} amplified. But no such effect has been measured, as far as I know.
}
} Frankly, I think we have a long way to go to explain the electron. In the
} mean time, I reserve the right to define my own understanding of it,
} although I may regret making those views public.
}
} On Wednesday, January 24, 2001 9:11 AM, Mike Bode
} [SMTP:mb-at-Soft-Imaging.com] wrote:
} } { { File: ATT00005.txt; charset = windows-1252 } }
}
} Allen,
}
} I am not sure I am following you. You say
}
} "The best high energy experiments have been unable to assign a 'mass' to
} the
} electron."
}
} To my knowledge and from a list of "fundamental constants" in my "Solid
} State Physics" book by Ashcroft and Mermin, the rest mass of an electron is
} 9.1095 x 10^(-31) kg. Just multiply that wit 1/2 v*v and you have the
} kinetic Energy of the electron. Of course you have to take into account
} relativistic effects. Or just multiply it with v and you have the momentum.
}
} I think, you mistake the mass of the electron with that of the neutrino,
} which indeed they have not been able to determine, although there are
} indications from recent high energy physics measurements. But that's
} irrelevant here.
}
} -----Original Message-----
} } From: Allen R. Sampson [mailto:ars-at-sem.com]
} Sent: Monday, January 22, 2001 4:21 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Question
}
} Yes, please forgive me. My spelling is not very accurate when I am
} responding so early in the morning. Your correction on Wehnelt is correct.
} Please forgive any such errors in this response, as it is similar in
} timing.
}
} The electric field of interest is not between any parts of the column, but
} rather between the electrons and the sample. I really don't want to try to
} attempt any quantum exercises here, but the energy impressed on the
} electrons results in their increased EM field effects. The acceleration of
} the electrons controlled by the electron gun configuration controls the
} energy afforded the electrons. What happens after the anode does not
} affect the energy of the electrons in the beam.
}
} The electrons are the field. The acceleration given to them by the gun
} determine their acceleration, and thus their field and relativistic
} effects. Their energy is determined by the fields in the gun structure
} alone. Once they are given that energy, their travel through the column is
} determined by the momentum they have been given. The fields they generate
} as they travel through the sample surface are the direct result of the
} energy they were provided with in the electron gun.
}
} Here's a simple challenge - define the 'kinetic' energy of a fast moving
} electron. As the kinetic energy is generally defined as the mass vs. the
} velocity of that mass, you may have a problem. The best high energy
} experiments have been unable to assign a 'mass' to the electron.
} Apparently, the only mass associated with the electron is the Einsteinium
} energy-mass equivalent of its charge. If there is any increase in an
} electron's mass/energy by acceleration in an applied electric field, it is
} to the electrons energy. Check with SLAC's experiments.
}
} A fine point, but one with merit. It doesn't matter whether the distance
} from anode to sample is 10 cm or 1000 cm, if the electron can travel the
} distance without interference and external force. Thus the electro-magn
} etic interaction of the electron with the sample is simply dependant on the
} force originally impressed on the electron by the gun.
}
} The field present at the sample surface will thus be determined by the
} acceleration given the electrons by the gun and their current density. The
} higher the beam current, the greater the field flux. The smaller the area
} of the electron impingement on the surface, the higher the field flux. As
} we work towards smaller circuit dimensions, these electron interactions
} produce higher field flux densities as the circuit dimensions come closer
} to the electron beam diameters.
}
} You seem to want to separate the mass related kinetic effects from the
} energy of the particle, but that can not be done. As the electron is
} accelerated, its energy is apparently increased, rather than its mass. The
} result is that the classical and relativistic effects normally related to
} mass are instead seen in an increase in the energy, and thus, the field
} effects of the electron with the sample.
}
} On Friday, January 19, 2001 10:43 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} wrote:
} } { { File: ATT00000.txt; charset = windows-1252 } }
}
} Thanks, Allen, for pointing out some of the issues here. By the way, It's
} "Wehnelt", not "Wienault".
}
} You are probably right about the ESD vs. EDS name. However, it was clear to
} me what the original question was about.
}
} Someone pointed out, that the cause of ESD (!) failure in electronic
} devices
} is, that the fields increase to beyond the breakdown strength of the
} devices, which causes a high and sudden current to flow through the parts
} where the fields are highest, which in turn heats and destroys the sample.
} I
} think, that is right. It's the fields that reach critical strength, which
} then causes the current flow and destruction of the device. That's what I
} meant when I said you get "zapped".
}
} Now, about the electron microscope. Obviously I don't want to pronounce,
} that electron microscopy is impossible. That would be stupid, wouldn't it?
} However, as you said yourself, the sample is at ground potential, or very
} near it, and so is the anode. In other words, there is not electric
} potential (or very little) between the anode and the sample and thus no
} electric field. Since the sample is grounded, as is the rest of the
} microscope, there is no electric field between those, either. The energy of
} an electron in an electric field is proportional to the electric field
} (actually proportional to the square of the field, if my memory serves me).
} No field - no electric energy. Of course that does not mean that the
} electrons don't have energy. They have quite a lot of energy, on the order
} of 20keV, by "falling" through the electric field between cathode and anode
} and not being stopped by the anode. Since the sample is grounded (or should
} be), the electric effects of the electron beam on the grounded sample will
} be cancelled. What you have to contend with is the kinetic energy (20
} keV/electron), which is transferred to the sample.
}
} Now, I have always talked about grounded samples. That does not mean that
} electric fields cannot be generated by the electron beam within the sample.
} Obviously, you put electrons in the sample and they have to move out of the
} sample. If there is any kind of resistance (an oxide layer, etc.), that
} will
} result in charging and an electric potential.
}
} -----Original Message-----
} } From: Allen R. Sampson [mailto:ars-at-sem.com]
} Sent: Friday, January 19, 2001 2:44 AM
} To: 'Mike Bode'
} Subject: RE: Question
}
} Ok, apparently a simple physics lesson needed here.
}
} First of all we are talking about ESD, or Electro-Static Discharge effects
} here. Not EDS, or what in this field is known as Electron Dispersive
} Spectroscopy.
}
} Secondly, the electrons arriving at the sample surface in an SEM are
} getting there with an acceleration that is roughly equivalent to the the
} acceleration voltage impressed at the cathode. The sample is at, or very
} near, ground potential. But the electrons in the beam are reaching the
} sample at close to the accelerating voltage impressed. The grid or
} "Wienault" of the electron gun is at a potential roughly +500V from the
} accelerating voltage and is used to induce the electrons from the cathode
} through the aperture in the anode, preventing the 'space charge' effects
} around the cathode and providing the first electrostatic lens in the gun.
}
} The potential of the anode of an electron microscope may be at ground
} potential, but the electron beam is passing through the opening in the
} anode. The anode is used as the second electrostatic lens, and has little
} effect on the energy of the electrons passing through it. By its charge
} opposite that of the electron beam, it encourages the first crossover of
} the beam, but since the beam does not directly interact with it, there is
} no direct energy exchange.
}
} Precisely what kinetic energy can be transferred to the sample if there is
} no energy differential between the electron potential at the anode and the
} sample? Given your understanding of the processes involved, there can be
} no discernable energy changes in the sample since the electrons have no
} energy, and thus, there can be no discernable energy release from the
} sample. In other words, in your view, electron microscopy is impossible.
}
} On Thursday, January 18, 2001 2:24 PM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} wrote:
} }
} } Yes, EDS is very real and chip manufacturers go to great length (end
} } expense) to avoid them. However, I think the mechanism is different:
} }
} } EDS means, that something collects static electricity (by walking on a
} } carpet, for example), then touches something that is at a very different
} } potential. That's what happens if you touch a metal after walking on that
} } carpet. The enormous potential difference create strong electric field
} that
} } finally ionize the air and you get "zapped". The strong fields can
} destroy
} } the electronics.
} }
} } In an SEM the sample is usually grounded and does not see strong fields.
} } True, the electrons are accelerated to 30 KV or more, but the sample does
} } not see that, as the anode usually sits at ground level with the Cathode
} } being at - 30 kV. What you need to be concerned about here is the kinetic
} } energy that is transferred to the sample and can heat it up considerable.
} On
} } the other hand, if the sample is not grounded, there will be fields
} } developing. There are also other effects, such as residual organics
} getting
} } "cracked" and forming a residue on the sample, but that's a different
} story.
} }
} } Michael
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
}
} On Wednesday, January 24, 2001 9:11 AM, Mike Bode
} [SMTP:mb-at-Soft-Imaging.com] wrote:
} } { { File: ATT00005.txt; charset = windows-1252 } }
}
} Allen R. Sampson, Owner
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
} voice 630.513.7093 fax 630.513.7092



From daemon Tue Jan 30 05:44:23 2001



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Tue, 30 Jan 2001 11:36:44 +0000 (GMT)
Subject: Re: Question about NIH Image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

You can find the NIH Image home page on:
http://rsb.info.nih.gov/nih-image/
The PC version is available from Scion Corporation on:
http://www.scioncorp.com

Cheers,

David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-msm.cam.ac.uk

On Mon, 29 Jan 2001, Eric wrote:

} Secondly, I need to know where I can find NIH Image if it is still
} available and is it availabe for the PC now??



From daemon Tue Jan 30 07:06:00 2001



From: MSimko-at-uss.com
Date: Tue, 30 Jan 2001 07:32:35 -0500
Subject: dusty Polaroid DMC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our laboratory owns a Polaroid DMC digital camera for acquiring digital
images from a Nikon Optiphot upright optical microscope. We have the need
to determine the cleanliness of polished steel samples and capture clean,
accurate digital images. This is a very demanding application and the
presence of any dust creates unacceptable spots on the final images.

The optics in the microscope and camera mounts have been checked multiple
times and are spotless. When critical images are needed, Polaroid film is
used which works wonderfully. Obviously we would prefer to capture the
images electronically. On visual inspection, the camera chip itself
appears to have dust on the surface. We have been told that we should not
attempt to service the unit ourselves and for about five hundred dollars we
could have factory service. However, we are also told that with the
mechanical shutter action, the problem will recur in short order.
Unfortunately, we cannot tolerate the loss of productivity taken by sending
the camera in for this kind of service at frequent intervals.

Does anyone have a similar problem with this camera? Can anyone suggest a
possible solution to this dilemma? I speculate that electrostatic forces
may be keeping the dust in place. Bursts of canned air will not remove the
dust but I would be willing to try something else. I am afraid that the
camera may not be adequate for these most delicate applications and we may
need to find another unit to meet our needs. Any assistance would be
greatly appreciated. Please contact me with any suggestions and I will
respond with the results. Thank you in advance for your help.

Michael Simko
Research Manager ? Metallography
U. S. Steel Research and Technology Center
msimko-at-uss.com




From daemon Tue Jan 30 07:20:22 2001



From: jshields-at-cb.uga.edu
Date: Tue, 30 Jan 2001 08:21:40 -0500
Subject: NIH image source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To my Fellow Microscopists,

First of all thanks for all the info on cleaning the LKB Knife boats
we are using here and I will try and get them to switch to dental
wax.
NIH Image is now Scion image and available for the PC.
You can find it here.
http://www.meyerinst.com/html/scion/scion_image_windows.htm



Secondly, I need to know where I can find NIH Image if it is still
available and is it availabe for the PC now??

Thanks

Eric A. Rosen
UCLA Medical Center
Electron Microscopy Lab
John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu


From daemon Tue Jan 30 08:50:55 2001



From: Zhaojie Zhang :      Zhaojie-Zhang-at-mail.omrf.ouhsc.edu
Date: Tue, 30 Jan 2001 08:43:10 -0600
Subject: Question about NIH Image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric:

NIH Image is still available and you can download the program at
http://www.scioncorp.com/

Zhaojie Zhang
Cell and Molecular Biology
Oklahoma Medical Research Foundation
OKC, OK 73104



-----Original Message-----
} From: Eric
To: 'Microscopy-at-MSA.Microscopy.Com'
Sent: 1/29/01 8:19 PM


To my Fellow Microscopists,

First of all thanks for all the info on cleaning the LKB Knife boats
we are using here and I will try and get them to switch to dental
wax.

Secondly, I need to know where I can find NIH Image if it is still
available and is it availabe for the PC now??

Thanks

Eric A. Rosen
UCLA Medical Center
Electron Microscopy Lab


{ { {This message is made of 100% recycled electrons} } }
|"|
-`^'- {_*_} ` _ , ' _|_|_
(o o) (o o) - (o)o) - (o o)
ooO--(_)--Ooo--8--(_)--Ooo-ooO'(_)--Ooo-ooO--(_)--Ooo
**Everyone has a photographic memory. Some just don't have film!
Eric {Los Angeles, California}
Go Check out my new and alomst finished webpage at:
http://www.geocities.com/Yosemite/Rapids/4855/
_ /|
\\\|///
\'o.O'
( o o )
==========oOO==(_._)==OOo==============oOO==(_)==OOo========



From daemon Tue Jan 30 13:51:13 2001



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Tue, 30 Jan 2001 09:26:03 -0600
Subject: Polaroid 55 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Although we have a digital camera
for our SEM, some people still
prefer Polaroids over digital
images. We've been using Polaroid
55 pos/neg film. We routinely wash
the negatives in running water for
at least 30 minutes to remove
residual traces of chemicals.
According to the literature
supplied with the film, the
negatives should cleared with 18%
sodium sulfite as soon as possible
after exposure, then briefly rinsed
in water. I assume that this is to
stop development and clear the
negative. My question is: Is sodium
sulfite a necessary step or can we
continue to clear with a long rinse
of running water? Note: we've never
seen any adverse effects from
washing in water alone.

Thanks in advance for your responses.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
Email: tom.januszewski-at-UTSouthwestern.edu


From daemon Tue Jan 30 13:51:17 2001



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Tue, 30 Jan 2001 14:28:59 -0500
Subject: Thanks for all the Cartoon Submissions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all who responded so generously with their emails, faxes and phone calls
- Thanks!

Cheers!
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Tue Jan 30 13:51:17 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Tue, 30 Jan 2001 10:38:20 -0500
Subject: Re: Batson's No 17 Plastic Replica and Corrosion Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

Since we do Mercox it would be unfair for Ladd to report our comparison of
Mercox with other corrosion casting materials.
But having said that, the work of our internal personnel and outside
researchers leads us to believe the problems incurred with other corrosion
casting materials in use have not been experienced with Mercox.
You may find the following links of interest,

http://laddresearch.com/Key_Products/Mercox/HosslerPaper/hosslerpaper.html

and

http://laddresearch.com/Key_Products/Mercox/mercox.html

Hope this was of use,

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



Gordon Vrololjak wrote:
}

} Hello,
} I have someone wanting to do some corrosion casts from parts of a rat. I
} was wondering if anyone has any experience with Batson's No 17 Plastic
} Replica and Corrosion Kit. I believe it is a methyl methacrylate monomer.
}
} We had trouble in the past in working with resins in the SEM. They tended
} to melt under the beam, even after sufficient coating. So I was hoping,
} to get any comments about this or other resins.
} Gordon.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793


From daemon Tue Jan 30 15:46:59 2001



From: Pradyumna Prabhumirashi :      p-prabhumirashi-at-northwestern.edu
Date: Tue, 30 Jan 2001 15:08:43 -0600
Subject: ZnO on Si (111)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I need to prepare a plan-view sample of a ZnO thin film (~200 nm) on Si
(111). I would like to etch away the Si and float the thin film on a grid.
Can someone please suggest me a way of doing this? I can etch the Si away by
HF/HNO3 but am afraid that in doing so, it would affect my ZnO too!. Please
advice.

Many thanks
Prad

Pradyumna Prabhumirashi
Department of Materials Science
Northwestern University
Phone: (847)-491-7798
Fax: (847)-491-7820
http://vpd.ms.northwestern.edu/prad.htm



From daemon Tue Jan 30 15:57:05 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 30 Jan 2001 13:53:02 -0800
Subject: Removable epoxy resist?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could you brainiacs please put on your thinking caps and see what you can
come up with for this problem?

A guy came to the lab asking if I knew of anything he could use as an
'epoxy resist'.

Now, here's the rest of the story:

He is making CCD's for telescopes. He wants to glue a piece of material to
the face of the CCD using a very low viscosity epoxy. He puts the two
together dry with thin strips of tape down two sides as spacers, then by
capillary action fills the space between with epoxy and lets it cure.

Sometimes excess epoxy runs out at the ends and covers some of the bonding
pads on the CCD. He wanted to know if there is something he could put on
the bonding pads that would shield them from the epoxy, either repel it or
protect the pads and let the epoxy separate easily from the pads after it
has cured.

It struck me that this is similar to the kinds of problems some material
scientists might run into when preparing TEM samples via tripod polishing,
ie. how to hold a sample, but then release it later.

Some more details. The bonding pads are 100 x 300 um made of 1 um thick Al.
After the gluing part, 1 mil gold wires are ultrasonically bonded to the
pads. He says the pads are too small to paint anything on, thinks a spray
might work. Pads can't be touched or the bonding won't work right.

I thought of super glue and acetone, ala tripod polishing. He wants to
avoid acetone and things like petroleum distallates. He thinks ethanol
and/or water would be OK, not sure about other solvents. I thought of wax
and xylene, but he wasn't sure about that one either. He took some sucrose
to try as a separation layer, he will try anything.

I suggested more careful measuring of the epoxy used so it wouldn't
overflow, but there are some technical problems that limit the usefulness
of this technique. He might try some physical barriers to hold back the
overflow, but he would still like a way to protect the pads from the epoxy
in case these fail.

So, is there anything that can be put on these pads to protect them from
epoxy and be removed later to open them up for bonding? If anybody knows,
one of you will.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Jan 30 16:36:50 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Tue, 30 Jan 2001 12:40:06 -0500
Subject: Museum Session at Scanning 2001

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues...

This note is to announce the session entitled "Museum Applications of SEM"
at Scanning 2001. Anyone involved in the application of scanning electron
microscopy and/or x-ray microanalysis in a museum setting (natural history,
art, geology, etc) is encouraged to submit an abstract.

Scanning 2001 will be held in New York City, May 5-7. Details for abstract
submission can be found at:

http://www.scanning.org/

Invited speakers are:

Frankie Jackson, Museum of the Rockies - "Dinosaur Eggshell Microstructure
Visualized by Scanning Electron Microscopy"

and

Mark Wypyski, Metropolitan Museum of Art - "SEM and X-ray Microanalysis
Applications for Art Materials"

All the best,

Angela

---------------------------------------------
Angela V. Klaus

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Voice: (212)769-5977
Fax: (212)496-3480
---------------------------------------------


From daemon Tue Jan 30 16:37:43 2001



From: Bob Bagnell :      rml-at-grayhawk.med.unc.edu
Date: Tue, 30 Jan 2001 17:33:41 -0400
Subject: black & white print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For years we have used a Kodak Dektomatic print processor (not to
be confused with the stabalization technology Ektomatic processor) for both
our EM negatives and prints. Polymax chemistry is fine for both. Kodak no
longer makes the Dektomatic, but a suitable replacement might be the Colex
Colette Pro B&W processor. We are working with Colex to determine if this
processor can handle EM film material. These processors are for relatively
high volume work (we process about 1500 film images per month) and are more
expensive than the MorePro machine.

Bob

_____________________________________________________________
C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof.
Microscopy Services Laboratory
Department of Pathology & Laboratory Medicine
CB #7525 UNC-CH, Chapel Hill, N.C. 27599
ph 919-966-2413 fx 919-966-6718
http://www.pathology.med.unc.edu/path/microscopy/welcome.htm




From daemon Tue Jan 30 16:47:50 2001



From: Peter Engelhardt :      Peter.Engelhardt-at-helsinki.fi
Date: Wed, 31 Jan 2001 00:05:53 +0200
Subject: Cryo-TEMs

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I wonder if someone could tell me rough approximate costs of:

- He-Cryo 300 kV, and Cryo 400, 200, or 120kV TEM,
- with FEG and equipments for automatic collection of tilt series,
CCD cameras etc.

I thank you in advance

Cheers

Peter





--
___________________________________________________________
Peter Engelhardt, PhD, docent
Assistant Professor in Molecular Genetics
Unit of Electron Tomography
Department of Virology
Haartman Institute
P.O.Box 21 (Haartmaninkatu 3)
FIN-00014 University of Helsinki,
Finland

Email: Peter.Engelhardt-at-Helsinki.FI
URL: http://www.csc.fi/jpr/emt/engelhar/
Mobil phone: 040 811 9180

Dept. Virology, Tel: (+358-9)-19126487,19126507
EM-laboratory: (+358-9)-19126885 (direct)
Fax: (+358-9)-19126491 (Dept of Virology)

CSC (Center for Scientific Computing):
Tekniikantie 15a D, Otaniemi, Espoo
CSC-facilities, Tel: (+358-9)-4573229 (direct)

Home address:
Lindstedstvägen 1 B 7
FIN-02700 Grankulla,
Finland
Home Tel: (+358-9)-5091381



From daemon Tue Jan 30 17:28:49 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Tue, 30 Jan 2001 18:29:11 -0500
Subject: cartoons for TEM illustration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am looking for some cartoons to illustrate what is a TEM. I would like
to make a poster to show the general public what is TEM.

I would appreciate very much if anyone could send me or tell me where to
get these cartoons (like a sketch of a microscope), anything related to TEM
would do.

Regards

Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Tue Jan 30 17:30:40 2001



From: Glenn Larkin :      gmlarkin-at-mtu.edu
Date: Tue, 30 Jan 2001 17:26:25 -0500
Subject: Boron Microanalysis (Biological Specimens)

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,

I am working on the analysis of boron in lignocellulosic materials,
employing an electron microprobe and synthetic WDS crystal (OV-145H).
Does anyone know what the lowest reported detection limit for boron in
biological matrix is? Any helpful suggestions?

Thank you.

Regards,


Glenn


Glenn M. Larkin
Research Scientist
School of Forestry & Wood Products
Michigan Technological University
Houghton, MI 49931-1295
Ph: (906) 487-3316
Fax: (906) 487-2915
e-mail: gmlarkin-at-mtu.edu



From daemon Tue Jan 30 19:32:56 2001



From: colin.veitch-at-tft.csiro.au
Date: Wed, 31 Jan 2001 10:38:46 +1000
Subject: NIH Image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

The PC version of Image is available from Scion Corp BUT there is platform
independent, Java version available from NIH at http://rsb.info.nih.gov/ij/
I have used both and personally, I prefer the Java version.

Cheers

Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-tft.csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811


The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.



From daemon Tue Jan 30 21:46:48 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 30 Jan 2001 20:57:22 -0600
Subject: Re: Removable epoxy resist?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shellac and alcohol?

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
} Could you brainiacs please put on your thinking caps and see what you
can
} come up with for this problem?
}
} A guy came to the lab asking if I knew of anything he could use as an
} 'epoxy resist'.
}
} Now, here's the rest of the story:
}
} He is making CCD's for telescopes. He wants to glue a piece of material
to
} the face of the CCD using a very low viscosity epoxy. He puts the two
} together dry with thin strips of tape down two sides as spacers, then by
} capillary action fills the space between with epoxy and lets it cure.
}
} Sometimes excess epoxy runs out at the ends and covers some of the
bonding
} pads on the CCD. He wanted to know if there is something he could put on
} the bonding pads that would shield them from the epoxy, either repel it
or
} protect the pads and let the epoxy separate easily from the pads after
it
} has cured.
}
} It struck me that this is similar to the kinds of problems some material
} scientists might run into when preparing TEM samples via tripod
polishing,
} ie. how to hold a sample, but then release it later.
}
} Some more details. The bonding pads are 100 x 300 um made of 1 um thick
Al.
} After the gluing part, 1 mil gold wires are ultrasonically bonded to the
} pads. He says the pads are too small to paint anything on, thinks a
spray
} might work. Pads can't be touched or the bonding won't work right.
}
} I thought of super glue and acetone, ala tripod polishing. He wants to
} avoid acetone and things like petroleum distallates. He thinks ethanol
} and/or water would be OK, not sure about other solvents. I thought of
wax
} and xylene, but he wasn't sure about that one either. He took some
sucrose
} to try as a separation layer, he will try anything.
}
} I suggested more careful measuring of the epoxy used so it wouldn't
} overflow, but there are some technical problems that limit the
usefulness
} of this technique. He might try some physical barriers to hold back the
} overflow, but he would still like a way to protect the pads from the
epoxy
} in case these fail.
}
} So, is there anything that can be put on these pads to protect them from
} epoxy and be removed later to open them up for bonding? If anybody
knows,
} one of you will.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}




From daemon Tue Jan 30 22:27:31 2001



From: mary mckee :      mckee-at-helix.mgh.harvard.edu
Date: Tue, 30 Jan 2001 22:21:57 -0600
Subject: Reichert accesories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Listers,

I need a couple of things for a Reichert ultracut E and wondered if anyone
knows a supplier. I need a couple of chuck holders (locks chuck in place
for trimming the block). Also, I need a rubber 'belt' to operate the
specimen arm - one broke. Thanks in advance.

Mary Mckee
Renal Unit
MGH
Charlestown, MA

(617)726-3696 phone
(617)726-5669 fax




From daemon Wed Jan 31 00:30:12 2001



From: Tony Bruton :      Bruton-at-nu.ac.za
Date: Wed, 31 Jan 2001 08:24:01 +0200
Subject: Re: Thanks for all the Cartoon Submissions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Paul

I trust that all of these EM cartoons are going to find their way onto some public access site where we can all see them - and perhaps use them to liven up our lectures !

Tony


Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website via:http:www.nu.ac.za
Email:bruton-at-nu.unp.ac.za
postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } "Gerroir, Paul J" {Paul.Gerroir-at-crt.xerox.com} 01/30/01 09:28PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To all who responded so generously with their emails, faxes and phone calls
- Thanks!

Cheers!
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com





From daemon Wed Jan 31 02:52:19 2001



From: jesper.v.carstensen-at-risoe.dk
Date: Wed, 31 Jan 2001 09:32:37 +0100
Subject: cartoons for TEM illustration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Yan Xin,

Here are a few suggestions:

Sketch of the first two-step electron microscope with magnetic lenses
(1931), designed and realized by M. Knoll and E. Ruska:
http://www.scandem.lu.se/

Learn how transmission electron microscopes work:
http://www.unl.edu/CMRAcfem/temoptic.htm

Study common uses of the TEM: http://www.mri.psu.edu/mcl/tem.htm

Modes of transmission electron microscope operation:
http://em-outreach.sdsc.edu/web-course/Sec-I.F/Sec-I.F.html

I hope that you can use some of it.

Kind regards,
Jesper

_ _
(. .)
---------------------000--(_)--000----------
Jesper Vejlø Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O.Box 49, DK-4000 Roskilde
DENMARK
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/afm
--------------------------------------------





-----Original Message-----
} From: Yan Xin [mailto:xin-at-magnet.fsu.edu]
Sent: 31. januar 2001 00:29
To: microscopy-at-sparc5.microscopy.com


Hello,

I am looking for some cartoons to illustrate what is a TEM. I would like
to make a poster to show the general public what is TEM.

I would appreciate very much if anyone could send me or tell me where to
get these cartoons (like a sketch of a microscope), anything related to TEM
would do.

Regards

Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Wed Jan 31 07:04:53 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 31 Jan 2001 04:58:39 -0800 (PST)
Subject: Re: Polaroid 55 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom:
It has been awhile since I used the 55p/n film, but IF memory serves me
correctly, the sodium sulfite was used to both stop the development process
and to harden the emulsion. We used this for years (as did many
microscopists) and finally switched over to positive only polaroid (when not
grabbing digital images).

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Tue, 30 Jan 2001 09:26:03 -0600, tom.januszewski-at-email.swmed.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Although we have a digital camera
} for our SEM, some people still
} prefer Polaroids over digital
} images. We've been using Polaroid
} 55 pos/neg film. We routinely wash
} the negatives in running water for
} at least 30 minutes to remove
} residual traces of chemicals.
} According to the literature
} supplied with the film, the
} negatives should cleared with 18%
} sodium sulfite as soon as possible
} after exposure, then briefly rinsed
} in water. I assume that this is to
} stop development and clear the
} negative. My question is: Is sodium
} sulfite a necessary step or can we
} continue to clear with a long rinse
} of running water? Note: we've never
} seen any adverse effects from
} washing in water alone.
}
} Thanks in advance for your responses.
}
} Tom Januszewski
} Senior Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} Dallas, TX 75390-9039
} 214-648-7291
} Email: tom.januszewski-at-UTSouthwestern.edu
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed Jan 31 07:06:26 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 31 Jan 2001 05:02:43 -0800 (PST)
Subject: Re: Reichert accesories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary:
Leica took over the Reichert line of products several years ago, so getting
in touch with the local Leica rep (I just saw that Energy Beam Sciences is
the rep here in New England) is your best bet. If that doesn't work try the
links at the MSA web site or at the Microworld Resource page
http://www.mwrn.com.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Tue, 30 Jan 2001 22:21:57 -0600, mary mckee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, Listers,
}
} I need a couple of things for a Reichert ultracut E and wondered if
anyone
} knows a supplier. I need a couple of chuck holders (locks chuck in place
} for trimming the block). Also, I need a rubber 'belt' to operate the
} specimen arm - one broke. Thanks in advance.
}
} Mary Mckee
} Renal Unit
} MGH
} Charlestown, MA
}
} (617)726-3696 phone
} (617)726-5669 fax
}
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed Jan 31 10:04:20 2001



From: dino-at-peterbosch.com
Date: 31 Jan 2001 07:58:04 -0800
Subject: Microscopist looking for a new position

Contents Retrieved from Microscopy Listserver Archives
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31 Jan 2001 07:58:04 PST
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Content-Disposition: inline
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X-Mailer: Web Mail 3.7.1.7

------------------------------------------------------------------------
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L.S.

Microscopist (34) (Msc) is looking for a new challenging working environment.
In total 10 years of research experience in materialscience/(fracture)mechanics.
3 years experience in SEM/EPMA/EBSD/AFM/Image-processing as well as confocal microscopy.
Currently working at University.

Interested?: mail dino-at-peterbosch.com


-------------------------------------
Register for your free domain name!
Plus free email and a personal portal
http://www.namedemo.com


From daemon Wed Jan 31 10:35:47 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 31 Jan 2001 08:30:24 -0800
Subject: Re: Removable epoxy resist?

Contents Retrieved from Microscopy Listserver Archives
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Dear Jon,
I would suggest the "Mold release spray" that I use to stop the epoxy
mounting material from sticking to to the molds. Mine is by Crown, number
63223 and is called "Ready Release", "Gerneral Purpose Silicone". Hope this
helps.
At 01:53 PM 1/30/01 -0800, you wrote:

} Could you brainiacs please put on your thinking caps and see what you can
} come up with for this problem?
}
} A guy came to the lab asking if I knew of anything he could use as an
} 'epoxy resist'.
}
} Now, here's the rest of the story:
}
} He is making CCD's for telescopes. He wants to glue a piece of material to
} the face of the CCD using a very low viscosity epoxy. He puts the two
} together dry with thin strips of tape down two sides as spacers, then by
} capillary action fills the space between with epoxy and lets it cure.
}
} Sometimes excess epoxy runs out at the ends and covers some of the bonding
} pads on the CCD. He wanted to know if there is something he could put on
} the bonding pads that would shield them from the epoxy, either repel it or
} protect the pads and let the epoxy separate easily from the pads after it
} has cured.
}
} It struck me that this is similar to the kinds of problems some material
} scientists might run into when preparing TEM samples via tripod polishing,
} ie. how to hold a sample, but then release it later.
}
} Some more details. The bonding pads are 100 x 300 um made of 1 um thick Al.
} After the gluing part, 1 mil gold wires are ultrasonically bonded to the
} pads. He says the pads are too small to paint anything on, thinks a spray
} might work. Pads can't be touched or the bonding won't work right.
}
} I thought of super glue and acetone, ala tripod polishing. He wants to
} avoid acetone and things like petroleum distallates. He thinks ethanol
} and/or water would be OK, not sure about other solvents. I thought of wax
} and xylene, but he wasn't sure about that one either. He took some sucrose
} to try as a separation layer, he will try anything.
}
} I suggested more careful measuring of the epoxy used so it wouldn't
} overflow, but there are some technical problems that limit the usefulness
} of this technique. He might try some physical barriers to hold back the
} overflow, but he would still like a way to protect the pads from the epoxy
} in case these fail.
}
} So, is there anything that can be put on these pads to protect them from
} epoxy and be removed later to open them up for bonding? If anybody knows,
} one of you will.
}
} Thanks
}
} Jonathan Krupp
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jan 31 10:58:14 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 31 Jan 2001 08:52:11 -0800
Subject: Re: Polaroid 55 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom,
I have used PN 55 for many years and always used the 18% sodium sulfite
bath. When you slip the negative into the sodium sulfite bath and gently
swish it around, the negative clears and the brown developing jelly lifts
off the negative and floats in the bath. It can then be fished out and
disposed of like your other film developers. I then rinse the negative for
10 to 15 minutes in running water to remove the remaining caustic. My
concern would be that if you only rinse in water, the developer goes down
the drain and many jurisdictions do not allow this.
At 09:26 AM 1/30/01 -0600, you wrote:
} Hi,
}
} Although we have a digital camera
} for our SEM, some people still
} prefer Polaroids over digital
} images. We've been using Polaroid
} 55 pos/neg film. We routinely wash
} the negatives in running water for
} at least 30 minutes to remove
} residual traces of chemicals.
} According to the literature
} supplied with the film, the
} negatives should cleared with 18%
} sodium sulfite as soon as possible
} after exposure, then briefly rinsed
} in water. I assume that this is to
} stop development and clear the
} negative. My question is: Is sodium
} sulfite a necessary step or can we
} continue to clear with a long rinse
} of running water? Note: we've never
} seen any adverse effects from
} washing in water alone.
}
} Thanks in advance for your responses.
}
} Tom Januszewski
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jan 31 12:29:05 2001



From: greg :      greg-at-umic.sunysb.edu
Date: Wed, 31 Jan 2001 13:25:12 -0500
Subject: Job Listings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Is there a site that has a listing of jobs for
E.M. Please respond off the list server. I don't
wish to clutter up the listserver with non
techinical request.
--
Best Regards,
Gregory Rudomen
Technical Specialist Electron Microscopy
631-444-7372 Greg-at-umic.sunysb.edu
*************************************************
Standard disclaimer: The opinions expressed
in this communication are my own and do
not necessarily reflect those of the University
Microscopy Imaging Center.
*************************************************


From daemon Wed Jan 31 13:58:52 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 31 Jan 2001 13:50:42 -0600
Subject: Re: Reichert accesories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Another person to try would be Dave Paschke, The Dawson Co.,
} (617)484-7900. He had some parts for our Reichert MeF2 when I spoke to
} him a year ago...you never know.
}
} Diane Ciaburri
} General Dynamics
} Pittsfield, MA 01235
} (413)494-2847

} your freind could try to contact Mr. Rasche in Germany:
} email: mikrovid-at-gmx.de
} homepage: www.mikrovid.com
}
} best wishes, Joachim
}
}
} Dr. Joachim Prutsch
} Product Manager EM Specimen Preparation
}
} Leica Microsystems
} Hernalser Hauptstr. 219 email:
} Joachim.Prutsch-at-leica-microsystems.com
} A-1170 Vienna Tel. +43 1 48899 - 235
} Austria Fax +43 1 48899 - 350
} ---------------------- Weitergeleitet von Joachim

These are my only source for Reichart parts.

Good luck
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Jan 31 14:40:45 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Wed, 31 Jan 2001 15:31:15 -0800
Subject: RE: Polaroid 55 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,
Here is verbiage from a Polaroid Tech Sheet on Type 55 film:


Processing the reusable negative:
In order to remove the reagent layer and the anti-halation dyes,
the processed negative needs to be washed in an 18% sodium sulfite
solution.
The salts within the solution minimize the swelling in the negative's
gelatin layer that would be caused by washing in water only. Swelling can
cause reticulation which would remain after the negative dries.

To prevent scratches:
Negative scratch resistance can be improved by treating the
processed negative(after clearing in water and sodium sulfite) in a
solution of Kodak Rapid Fix with Hardener (parts A&B) for two minutes.
This solution should be made up and used in accordance with Kodak's
recommended mix procedures, chemical caution statements, wash times
and temperatures.

Warm Water 2 liters 70oz.
Soduim Sulfite anhydrous 440 grams 16oz(avdp)


George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com





From daemon Wed Jan 31 16:10:32 2001



From: William P. Sharp :      wsharp-at-asu.edu
Date: Wed, 31 Jan 2001 15:03:51 -0700
Subject: TEM Cryo Stages

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello again, list -

Seems I only communicate when I need something. Our Life Science EM lab is
considering writing a proposal for a cryo stage for our Philips CM12S. We
have a range of support instrumentation such as a high pressure freezer,
cryo ultramicrotomy atttachment for one of our ultramicrotomes, plus other
bits and bobs. We do a lot of freeze substitution, but there are times when
it would be useful in the extreme to see material that has been frozen only
- not subjected to immersion in solvents, chemical fixatives, etc.

To that end, we have had discussions with one or more companies who build
and/or sell cryo transfer and cryo stages or specimen holders for our
research microscope. Some offer what are characterized as "additional" or
"ancillary" anti contamination blades or devices that are permanently
installed in the objective lens gap ( if there's room) and are used to keep
contaminants from settling on the specimen (which is the coldest spot in
the immediate area) . We are told that these devices necessarily cost a
great deal and may not even be useable if there is too much "stuff" - like
detectors, etc. in the gap or if the gap is too small.

Our 'scope has a short focal length lens called a "twin lens" (as opposed
to a super twin or an ultra twin or a bio twin) and we have an EDS
detector, a secondary electron detector and its bias plate, a stock
anticontamination horseshoe, and the specimen holder plus the objective
aperture all in the vicinity.

My questions. Finally. First, is it folly to expect excellent results using
a cryo holder without the extra anticontamination blades installed? I am
thinking in terms of long exposure periods such as one would experience if
one were trying to learn TEM Tomography - tilt series micrographs through
short intervals over the full range of the holder. Second, if the blades
are installed, do they limit the use of other detectors or the movement
range of the specimen or any other function that I haven't thought of. I'm
hoping there are those of you out there who have the blades and can comment
and those of you who only have the transfer device and holder who can relay
their experiences and some hint as to the quality of image achieved and the
difficulty encountered either with the system or because of it.

Ours is a multi-user facility and we are trying to add to the functionality
of our instruments, not to make them less useful.

Thanks very much for any comment or observation, whether on or off list.

Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Wed Jan 31 16:10:33 2001



From: Bob Price :      price-at-dcsmserver.med.sc.edu
Date: Wed, 31 Jan 2001 17:04:19 -0500
Subject: Microscopy and Microanalysis 2001 meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Members,

The program for the Microscopy and Microanalysis 2001 meeting that
will be held in Long Beach, California from August 4-9 promises to be
an exciting one and covers many aspects of light, confocal, atomic
force and electron microscopy. Highlights of the scientific meeting will
include a 2-day Pre-meeting Congress chaired by Dr. Dave Piston of
Vanderbilt University entitled "Imaging Life: From Cells to Whole
Animals", a number of pre-meeting workshops, and a variety of
symposia in the Biological and Material sciences covering specific
topics on the development and applications of a full range of
microscopes and microscopy techniques.

Contributed presentations and posters are welcome for all scientific
sessions. Deadline for submission of abstracts (2 page extended format)
is February 15, 2001.

In addition to the scientific program the Local Arrangements Committee,
chaired by Bob Koch and Zed Mason, have arranged several activities
around the Long Beach area. These include the Sunday Golf
Tournament, a Sunday evening reception on the Queen Mary, and a
Wednesday Evening cruise around the Long Beach and Los Angeles
breakwater on the motor yacht "Spirit".

Full information concerning the meeting including the instructions for
submitting abstracts, registration forms and lodging, can be found by
following the Microscopy and Microanalysis '01 link on the right side of
the Microscopy Society of America web page
(www.msa.microscopy.com). If there are any questions please contact
me (Price-at-med.sc.edu) or the meeting managers (877-MSA-MAS-1).

I hope to see as many of you as possible in Long Beach.

Bob Price


Bob Price
M&M 2001 Program Chair
803-733-3393 (T)
803-733-1533 (F)


From daemon Wed Jan 31 16:30:32 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 31 Jan 2001 14:25:11 -0800
Subject: Re: Thanks for all the Cartoon Submissions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I'm familiar with the cartoon that Paul wanted; we tried to get permission
to use it for a t-shirt for the international EM meeting in Seattle a few
years ago. But even though the artist lived there (and was even a neighbor
of the then-current MSA president), the answer was a firm NO. Professional
cartoonists make a living from their drawings and they won't like "public
access". But MSA has a few talented amateurs, including its current
president (hi, Ron!), who could contribute; the job will get done if
someone (like you, for example) volunteers to do it!

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Jan 31 17:04:34 2001



From: Heinrich Matthies :      hjmatthies-at-ucdavis.edu
Date: Wed, 31 Jan 2001 16:59:45 -0600
Subject: rotary shadowing, platinum coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello,

I'm plan to attempt to rotary shadow a motor protein using platinum on a
carbon rod and then coat with carbon. At the moment, I am trying to
evaporate the platinum from the carbon electrode and am having difficulties.

We have a denton vacuum LLC with a bench top turbo III high vacuum
evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) and
then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm carbon
rod. The wire is pushed toward the solid carbon rod coming from the other
side and all of the loops are tight (touch each other). The "spring" of Pt
wire is tightly wound around the carbon wire. We pull a vacuum to about 5
x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and then
increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but
usually somewhere between 26-30, the carbon appears to evaporate. At lower
amps, less than 24, I don't see any Pt on the test paper. So at the higher
amps, both carbon and pt evaporate and this means we are getting too much
carbon rather than an initial Pt coating. Under these conditions, the Pt
wire only covers a short distance of the narrow tip of the carbon
electrode. Does anyone have any advice or experienced this problem?

Thank you,

Heiner Matthies
UC Davis
MCB
530-754-9051




From daemon Wed Jan 31 17:04:37 2001



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Wed, 31 Jan 2001 16:57:57 -0600
Subject: autoradiography for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

Is anyone out there working with autoradiography at the TEM level? We are
considering a project of radiolabelling plant cells and looking at the sites
of incorporation as per Pickett-Heaps, 1968 Protoplasma 65: 181-205.

I would be interested in any feedback regarding techniques, pitfalls,
references (current) etc. especially with regard to plants.

Thank you in advance,

Kim
---
Dr. Kim Rensing
Dept. of Botany, UBC
6270 University Blvd
Vancouver, BC, Canada
V6T 1Z4

phone: 604-822-5223
fax: 604-822-6089




From daemon Wed Jan 31 17:10:47 2001



From: Smartech :      smartech-at-javanet.com
Date: Wed, 31 Jan 2001 17:05:24 -0600
Subject: Nikon 990, what is the best set-up , focus method and monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have played w/ my Nikon 990 a while. I am very impressed with the camera.

I have a metallurgical and a stereo microscope and I connect the Nikon using
an eyepiece adaptor.

My question is: what is the best focus method and what is a suitable
external monitor.

I would assume a manual focus set at some reasonable focal length, perhaps
the distance the eye would perceive an object when viewed in the eye piece.
They fine focus could be done with the LM?

An external monitor seems to be required. The manual indicates NTSC or PAL
as video output. I assume that means only low resolution output, so no need
to spend extra for a high resolution monitor? Also, the LCD monitor
indicates 110,000 dots. Which I guess would be in the ball park of 300
lines and 400 pixels, so it is hard to image Nikon would put much technology
to produce extra resolution for the video output since only a small fraction
of users would ever connect to a monitor.

Has anyone out picked a monitor they are happy with?

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756





From daemon Wed Jan 31 17:10:47 2001



From: Smartech :      smartech-at-javanet.com
Date: Wed, 31 Jan 2001 17:05:43 -0600
Subject: used stereo microscpe pricing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is a fair price range for a used ( {10 years old and in good condition)
German or Swiss stereo microscope that can provide good resolution images up
to 100X. I am looking to buy such a scope. Please contact me directly if
you are selling a scope that fits this description.

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756




From daemon Wed Jan 31 19:04:53 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Wed, 31 Jan 2001 18:26:51 -0600
Subject: FW: Polaroid 55 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom:

For years and years, at least 20, we have been doing what you
do-rinsing in 20C water. 20 year old negatives are just as clear as new ones
with no signs of degradation.

Sam Purdy
Tech Center
National Steel Corp.
Trenton, MI

} From: Tom Januszewski
} Sent: Tuesday, January 2001, 10:26 AM
} To: microscopy-at-msa.microscopy.com
} Subject: Polaroid 55 film
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Although we have a digital camera
} for our SEM, some people still
} prefer Polaroids over digital
} images. We've been using Polaroid
} 55 pos/neg film. We routinely wash
} the negatives in running water for
} at least 30 minutes to remove
} residual traces of chemicals.
} According to the literature
} supplied with the film, the
} negatives should cleared with 18%
} sodium sulfite as soon as possible
} after exposure, then briefly rinsed
} in water. I assume that this is to
} stop development and clear the
} negative. My question is: Is sodium
} sulfite a necessary step or can we
} continue to clear with a long rinse
} of running water? Note: we've never
} seen any adverse effects from
} washing in water alone.
}
} Thanks in advance for your responses.
}
} Tom Januszewski
} Senior Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} Dallas, TX 75390-9039
} 214-648-7291
} Email: tom.januszewski-at-UTSouthwestern.edu
}




From daemon Wed Jan 31 19:20:58 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 31 Jan 2001 14:33:13 -0800
Subject: HV tank oil disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

You all did so well with the last question (epoxy resist) that I couldn't
resist trying a new one on you.

My EH&S guy wants to know about the current knowledge regarding the
disposal of HV tank oil that may be contaminated with PCB's. He has an HV
tank from a Philips x-ray machine, circa 1970's, rated at 100KV. He asked
me what is usually done to dispose of these tanks. The information he finds
is ambiguous. In some cases if the oil is drained, it can be disposed of
one way that may be cheaper than getting rid of the whole thing intact. On
the other hand, if he can just get rid of the whole thing as one piece it
would be a lot less mess.

Its the PCB's in the oil that complicates things. They were put in most HV
oils of that vintage, primarily to reduce the flamability of the simple
mineral oil used in the tank (I think). He would like to avoid an assay for
PCB's just to tell him something we already know. Also, if there is really
solid evidence and reasons for special care and caution with th oil, it
would help him chart the best course for responsible disposal.

Thanks,

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Jan 31 19:29:17 2001



From: Jane LaGoy :      jlagoy-at-bodycote-imt.com
Date: Wed, 31 Jan 2001 13:38:54 -0500
Subject: Re: EM cartoons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To second Paul Gerroir's request for the Gary Larson SEM cartoon, can anyone
e-mail or FAX one to me? I had forgotten that I had it, and another Gary
Larson one involving a light microscope attached to a car steering wheel as
a 'cool option', on the wall near our SEM. Then we had an explosion that
ruined my lab and SEM -- and the cartoons. (The lab has since been rebuilt
& SEM replaced.)

Thanks!

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
FAX 978-475-2951
jlagoy-at-bodycote-imt.com



From daemon Wed Jan 31 20:11:04 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 31 Jan 2001 15:43:18 -1000 (HST)
Subject: Re: HV tank oil disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Jon and Listers-

} My EH&S guy wants to know about the current knowledge regarding the
} disposal of HV tank oil that may be contaminated with PCB's. He has an HV
} tank from a Philips x-ray machine, circa 1970's, rated at 100KV. He asked
} me what is usually done to dispose of these tanks. The information he finds
} is ambiguous. In some cases if the oil is drained, it can be disposed of
} one way that may be cheaper than getting rid of the whole thing intact. On
} the other hand, if he can just get rid of the whole thing as one piece it
} would be a lot less mess.

{snip}

We managed to talk our local electric company into taking our old oil off
our hands - it was a miniscule amount for them. It was rather under the
table, to avoid all the inevitable paperwork, so call in any favors you
can think of... They are, however, prepared to deal with HV oil with PCBs
in it.

Good luck!
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jan 31 23:17:25 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 31 Jan 2001 23:10:52 -0600
Subject: Administrivia: Network Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

If things appear quiet or problematic with the Listserver it is due to
network problems. Connectivity will be sparse to intermittent
at best for the next few days until we sort out what is wrong.
As far as I can tell the problem is "outside" my local LAN.
The worrying thing is that it may be somewhere under the snow
and ice.

Nestor
Your Friendly Neighborhood SysOp




From daemon Thu Feb 1 00:38:06 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Thu, 1 Feb 2001 10:20:56 +0200
Subject: Microscopy of Invertebrate Reproduction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jon,

PCB's were a real concern even in the early 70's.
Your oil tank may or may not have PCB's at all as I believe they were banned
at that time.
The procedure we used (in the early 70's) was to first confirm the PCB's by
testing.
Several outside labs tested for a modest fee.

I really don't know who would test nowadays.
Maybe someone on the listserver would know.

Earl


----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 31, 2001 2:33 PM


At Oklahoma State they have a group that disposes of what ever you have as
long as you know what it is and were it came from. You don't need to know
if it has in it just the source. High voltage oil would be good enough for
them. They do the testing if needed. It is a lot cheaper for the
university for one office to deal with it than a bunch.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 31, 2001 4:33 PM


TO: THOSE INVOLVED IN MICROSCOPY OF INVERTEBRATES

CONFERENCE ANNOUNCEMENT

The 9th International Congress on Invertebrate Reproduction &
Development is to be held in South Africa (Rhodes University,
Grahamstown) from July 15-20th. This integrative meeting
welcomes papers on all aspects of invertebrate reproduction. The
second circular with details is available at the conference web site:
http://www.rhodes.ac.za/conferences/icird2001 where it is also
possible to register online. The deadline date for registration and
submission of abstracts is March 31st after which a late fee will
apply. The current exchange rate is very much in favour of overseas
delegates. With registration delegates get entrance to all
conference sessions, daytime refreshments, lunches, conference
literature and evening functions. Delegates can also book
excursions to some of the local game parks where the "big five"
can be seen in malaria-free reserves. The International Society for
Invertebrate Reproduction was founded in 1975 - come to Africa to
celebrate 25 years of the society. We want to know about your
research on invertebrate reproduction.

If you require further information please do not hesitate to contact
the conference organiser, Alan Hodgson (A.Hodgson-at-ru.ac.za)

Alan Hodgson
--------------------------------------
Professor Alan Hodgson
Dept. Zoology & Entomology
Rhodes University
Grahamstown 6140
South Africa
Tel. (+46) 6038526 Fax (+46) 6224377
Cellphone 083 598 4892

Convener 9th International Conference on Invertebrate Reproduction
& Development 15-20 July 2001. For information visit:
http://www.rhodes.ac.za/conferences/icird2001/


From daemon Thu Feb 1 03:35:58 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 01 Feb 2001 10:41:30 +0100
Subject: EM Meeting in Stockholm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Date: Thu, 01 Feb 2001 10:25:14 +0100
} To: "Bob Price" {price-at-dcsmserver.med.sc.edu} ,
microscopy-at-sparc5.microscopy.com, confocal-at-listserve.acsu.buffalo.edu
} From: Gareth Morgan {Gareth.Morgan-at-impi.ki.se}
} Subject: EM Meeting in Stockholm
} In-Reply-To: {041b81404221f11SERVICES-at-connect.med.sc.edu}
}
} Bob
}
} Thanks for the meeting announcement. Have you seen this one?
}
} http://www.biosci.ki.se/SCANDEM2001/
}
} I hasten to add that I have no association with the conference. The
conference scientific programme looks interesting and Sweden and Stockholm
are worth a look. I know - I moved here 2 years ago.
}
}
}
}



Med vänliga hälsningar/With best regards

Gareth

http://www.ki.se/biomedlab

Tel +46 8 728 3734
Fax +46 8 728 3688
e-mail Gareth.Morgan-at-impi.ki.se

"Words are the seeds of misunderstanding - use them carefully."

"Give us the wisdom to know and not to feel that not knowing is less than
wisdom itself."

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

NB/Obs! Visiting address =
Lindhagensgatan 92
Kungsholmen


From daemon Thu Feb 1 05:13:18 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 1 Feb 2001 04:59:42 -0600
Subject: RE: Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl,

All due respect, but I'm not sure that anyone really has an answer to her
question. I've had responses that said ESD effects are very similar to
electron beam damage and I've had others who have said not. Frankly, I'm
confused on the issue and would love some definitive response. Have one to
offer?

On Monday, January 29, 2001 2:05 PM, Earl Weltmer [SMTP:earlw-at-pacbell.net]
wrote:
}
} With all due respect (long pause): Isn't this thread getting a bit old?
}
} The person who asked this question has long since had her question
answered.
}
} Doesn't this remind one of two dogs and only one tree?
}
} Thank You,
}
} Earl
}
} }
} } Allen R. Sampson, Owner
} } Advanced Research Systems
} } 317 North 4th. Street
} } St. Charles, Illinois 60174
} } voice 630.513.7093 fax 630.513.7092
} }
} }
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Feb 1 05:44:58 2001



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Thu, 1 Feb 2001 17:40:02 +0600
Subject: jem4000EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear LISTERS-FRIENDS,
Thank your very much to everyone for your kind replies on my problems
with electron miocroscope JEM-4000EX.

There was problem in valve V4: the leak in the place of welding of
internal bellow. This leak exist in open condition of valve only.
We have changed this valve on new and now everything is OK.
Again thank very much for help.
Looking forward to help YOU in future.

Sincerely yours
Anton Gutakovskii
Laboratory of Electron Microscopy
Institute of Semiconductor Physics
Novosibirsk, Russia
-
mailto:gut-at-thermo.isp.nsc.ru




From daemon Thu Feb 1 07:12:04 2001



From: Gareth Robinson :      Gareth.Robinson-at-emitech.co.uk
Date: Thu, 1 Feb 2001 10:24:08 -0000
Subject: RE: rotary shadowing, platinum coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Heinrich,

Can I refer you to an excellent article in Microscopy Today Issue 01-1.
Page 28 (Rotary Shadowing Macromolecules) By Douglas R Keene.

drk-at-shcc.org

Hope it helps

Kind regards

Gareth Robinson

Gareth Robinson


Emitech Ltd - Serving the Science of Electron Microscopy
South Stour Avenue
Ashford
Kent - TN23 7RS
United Kingdom
Tel +44 (0) 1233 646332
Fax +44 (0) 1233 640744
Email:EM-at-emitech.co.uk
HTTP://www.emitech.co.uk

This communication contains information which is confidential and may
also
be privileged. It is for the exclusive use of the addressee. If you
are
not the addressee, please note that any distribution, dissemination,
copying
or use of this communication or the information in it is prohibited, and
may
be unlawful. If you have received this message in error please advise
the
sender


} -----Original Message-----
} From: Heinrich Matthies [SMTP:hjmatthies-at-ucdavis.edu]
} Sent: 31 January 2001 23:00
} To: Microscopy-at-sparc5.microscopy.com
} Subject: rotary shadowing, platinum coating
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
}
}
} Hello,
}
} I'm plan to attempt to rotary shadow a motor protein using platinum on
} a
} carbon rod and then coat with carbon. At the moment, I am trying to
} evaporate the platinum from the carbon electrode and am having
} difficulties.
}
} We have a denton vacuum LLC with a bench top turbo III high vacuum
} evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045)
} and
} then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm
} carbon
} rod. The wire is pushed toward the solid carbon rod coming from the
} other
} side and all of the loops are tight (touch each other). The "spring"
} of Pt
} wire is tightly wound around the carbon wire. We pull a vacuum to
} about 5
} x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and
} then
} increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but
} usually somewhere between 26-30, the carbon appears to evaporate. At
} lower
} amps, less than 24, I don't see any Pt on the test paper. So at the
} higher
} amps, both carbon and pt evaporate and this means we are getting too
} much
} carbon rather than an initial Pt coating. Under these conditions, the
} Pt
} wire only covers a short distance of the narrow tip of the carbon
} electrode. Does anyone have any advice or experienced this problem?
}
} Thank you,
}
} Heiner Matthies
} UC Davis
} MCB
} 530-754-9051
}
}


From daemon Thu Feb 1 08:00:14 2001



From: Gary Bassell :      bassell-at-aecom.yu.edu
Date: Thu, 1 Feb 2001 09:52:19 -0500
Subject: job ad for EM tech

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't wish to add to the "tree".


Earl
----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Earl Weltmer'" {earlw-at-pacbell.net} ; "'Mike Bode'"
{mb-at-Soft-Imaging.com} ; "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 01, 2001 2:59 AM


Please post the following announcement:

POSITION AVAILABLE FOR EM TECHNICIAN
The project involves localization of mRNA, cytoskeletal proteins and
synaptic markers in neurons using in situ hybridization and
immunogold methods. Expertise in immunogold and TEM desired. State
of the art microscopy facility. Highly competitive salary and
benefits.

Please send CV to:

Dr. Gary Bassell
Department of Neuroscience
Kennedy Center for Mental Retardation, #529
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461
Tel 718-430-3648
FAX 718-430-2960
--
------------------------------------
Gary Bassell, Ph.D
Assistant Professor
Department of Neuroscience
Rose Kennedy Center for Mental Retardation, Room #529
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461
Tel 718-430-3648
FAX 718-430-2960
http://www.aecom.yu.edu/asb/bassell/bassell.htm


From daemon Thu Feb 1 11:00:10 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 1 Feb 2001 08:55:04 -0800
Subject: PCB's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI:

Found a web site with everything you need to know about PCB's

http://www.llnl.gov/es_and_h/guidelines/pcb/pcb.html.

Take a look.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Feb 1 12:22:15 2001



From: Darcy Kehler :      kehler-at-twu.ca
Date: Thu, 1 Feb 2001 10:16:33 -0800
Subject: Requesting biological TEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,

I am seeking surplus TEM specimens for demonstration of EM to undergrad
Biology students. We have acquired a Philips EM 300 which is operational
but we would like to be able to show a range of biological preparations to
our students.
Currently we have no biological specimens whatsoever.

I would be grateful for any prepared grids which might be spared.

A brief description of a grid including a specimen's identity and perhaps
the method of preparation would be useful.

Please reply off list if you might be able to assist.
Thanks.

Darcy

Mr. Darcy Kehler, ext 3249
Lab Co-ordinator, Biology
Trinity Western University
7600 Glover Rd
Langley, BC
Canada V2Y 1Y1
Switchboard: (604) 888-7511
Secretary: (604) 513-2043
fax (604) 513-2018


From daemon Thu Feb 1 13:21:22 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 2 Feb 2001 08:23:00 GMT+1200
Subject: Re: HV tank oil disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} My EH&S guy wants to know about the current knowledge regarding the
} disposal of HV tank oil that may be contaminated with PCB's. He has
} an HV tank from a Philips x-ray machine, circa 1970's, rated at
} 100KV. He asked me what is usually done to dispose of these tanks.
} The information he finds is ambiguous. In some cases if the oil is
} drained, it can be disposed of one way that may be cheaper than
} getting rid of the whole thing intact. On the other hand, if he can
} just get rid of the whole thing as one piece it would be a lot less
} mess.
}
} Its the PCB's in the oil that complicates things. They were put in
} most HV oils of that vintage, primarily to reduce the flamability of
} the simple mineral oil used in the tank (I think). He would like to
} avoid an assay for PCB's just to tell him something we already know.
} Also, if there is really solid evidence and reasons for special care
} and caution with th oil, it would help him chart the best course for
} responsible disposal.
}


You should first of all check with Philips, they are pretty together
with info on that.

Electricity supply companies use a proprietary test kit to determine
if there are PCBs in transformer oil, so contact your local.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Feb 1 14:43:42 2001



From: Sally Shrom :      sally.shrom-at-villanova.edu
Date: Thu, 01 Feb 2001 15:33:37 -0500
Subject: darkroom/enlarger

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I have a Durst Laborator 1200 varipoint enlarger. The Varicontrol 1200
frequently blows fuses. I am using a 1 amp slow blow fuse. The point
source bulb is 100 watt. Can I get a replacement Varicontrol?
Thank you,
Sally Shrom



From daemon Thu Feb 1 15:46:01 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 01 Feb 2001 15:41:05 -0600
Subject: Re: darkroom/enlarger

Contents Retrieved from Microscopy Listserver Archives
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I would suggest looking at the power rating on the control unit. A 120-volt
100 watt bulb should pull 0.83 amps. That does not leave much margin or
room for anything else. I would look at a 1.5A fuse if the unit rating
permits it.

A on 120 V At 03:33 PM 2/1/2001 -0500, you wrote:

} Hello,
} I have a Durst Laborator 1200 varipoint enlarger. The Varicontrol 1200
} frequently blows fuses. I am using a 1 amp slow blow fuse. The point
} source bulb is 100 watt. Can I get a replacement Varicontrol?
} Thank you,
} Sally Shrom

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Thu Feb 1 17:10:31 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 2 Feb 2001 09:59:15 +1000
Subject: RE: rotary shadowing, platinum coating

Contents Retrieved from Microscopy Listserver Archives
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Dear Allen,

The person who asked the inital question, Ginny Harper, is a customer of
mine.
She called me the day after her question & I directed her to proper,
knowledgable, people at Motorola.
As far as I know, her question has been answered to her satisfaction.

The last two emails were not directed at you personally.
Like many on this Listserver I don't read every subject & delete the
irrelevent ones as I am very busy.
I took note at this one because a customer of mine asked the initial
question.

I was surprised at the tangent(s) that this thread has generated none of
which relate to her question.
I believe this forum was meant to share collective knowledge & experiences.
It appears to have degenerated to a test of "Will & Egos".

I decline your invitation to comment on said "ESD effects".
I am an SEM repairman & "ESD effects" is not my area of expertise:
nor is the subject of "quantum mechanics". Even the experts in the field of
quantum mechanics dis-agree with each other. (I know this first hand).
I will comment only on the areas of expertise that I feel I am qualified to
comment.
I will, however, direct you to people who are more qualified to answer that
question as it is their business.


Regards,


Earl Weltmer


The last two comments were
----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Earl Weltmer'" {earlw-at-pacbell.net} ; "'Mike Bode'"
{mb-at-Soft-Imaging.com} ; "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 01, 2001 2:59 AM


In order to meet federal requirements, the oil should be tested for PCB
content. A result of less than 50 mg/kg is considered non-PCB. A
concentration that 50 mg/kg to 499 mg/kg is considered to be contaminated
with PCBs. And a concentration of 500 mg/kg or greater is considered to be
PCB. There is an entire code in the Federal Register that deals with the
proper way to dispose of these types of oil. If the concentration is found
to be 50 mg/kg or greater, the oil is considered to be Hazardous Waste, and
falls within the RCRA standard. The following URL will take you to the
EPA's PCB Home Page where your friend will find several links to answer his
questions.

http://www.epa.gov/opptintr/pcb/

Hope this helps.

Regards,
Beth Bray

Elizabeth P. Bray
Plant Chemist, Central Laboratory
South Carolina Electric and Gas Co.
2102 N. Lake Dr.
Columbia, SC 29212

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 31, 2001 5:33 PM


I translate (x25.4) the funny measure 0.008 " to 0.2mm.
For Pt/C coating the more commonly used thickness is 0.1mm wire. If you work
out the cross section area of both wires you would find that you are using
considerably more Pt than the 50 to 75mm of 0.1mm wire otherwise used.
I suggest that you switch to the thinner wire because this would cover more of
the 1.4mm carbon cylinder. Therefore this would take longer (in time) to
evaporate. This is important because you need more than 5 seconds of
evaporation to obtain an even coating - with the specimen rotating at perhaps
30 RPM.
What do you mean: " } at lower amps, less than 24, I don't see any Pt on the
test paper"?
The C would be much more visible than the Pt and normally you would not see the
Pt on filter paper.
The test is in the looking.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, February 01, 2001 9:00 AM, Heinrich Matthies
[SMTP:hjmatthies-at-ucdavis.edu] wrote:
}
}
} Hello,
}
} I'm plan to attempt to rotary shadow a motor protein using platinum on a
} carbon rod and then coat with carbon. At the moment, I am trying to
} evaporate the platinum from the carbon electrode and am having difficulties.
}
} We have a denton vacuum LLC with a bench top turbo III high vacuum
} evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) and
} then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm carbon
} rod. The wire is pushed toward the solid carbon rod coming from the other
} side and all of the loops are tight (touch each other). The "spring" of Pt
} wire is tightly wound around the carbon wire. We pull a vacuum to about 5
} x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and then
} increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but
} usually somewhere between 26-30, the carbon appears to evaporate. At lower
} amps, less than 24, I don't see any Pt on the test paper. So at the higher
} amps, both carbon and pt evaporate and this means we are getting too much
} carbon rather than an initial Pt coating. Under these conditions, the Pt
} wire only covers a short distance of the narrow tip of the carbon
} electrode. Does anyone have any advice or experienced this problem?
}
} Thank you,
}
} Heiner Matthies
} UC Davis
} MCB
} 530-754-9051
}
}



From daemon Thu Feb 1 18:47:25 2001



From: u980013 :      u980013-at-giki.edu.pk
Date: Fri, 2 Feb 2001 05:44:55 +0500
Subject: Lithography information needed

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I am a student doing a Research paper on the subject ofLithography and
Semiconductor Fabrication
I need information on the subject of=20
Lithography....Its types specially Photolithography,
Electron beam Lithograaphy, UV lithography and Xray lithography
Plz help me...if u have any articleds plz send them to me...
or tell me of WEB Resources that can help..
plz reply soon
u980013-at-giki.edu.pk




From daemon Thu Feb 1 19:47:54 2001



From: rad0 :      rden25-at-mindspring.com
Date: Thu, 1 Feb 2001 19:27:52 -0600
Subject: test

Contents Retrieved from Microscopy Listserver Archives
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test



From daemon Thu Feb 1 19:53:00 2001



From: rad0 :      rden-at-mindspring.com
Date: Thu, 1 Feb 2001 19:50:31 -0600
Subject: test

Contents Retrieved from Microscopy Listserver Archives
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test



From daemon Thu Feb 1 20:13:15 2001



From: Gramp Skin Pathology :      grampath-at-camtech.net.au
Date: Fri, 2 Feb 2001 09:36:54 +1030
Subject: LM. CD 34 help

Contents Retrieved from Microscopy Listserver Archives
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I have used CD 34 QBend10 clone, to demonstrate endothelial cells for
several years. It gave a clean picture with no problems.

3 months ago it started to pick up collagen, elastic and general connective
tissue at such a high intensity that the technique became almost unusable.

I have changed the primary antibody and every other reagent involved in the
technique. I have also tried changing the dilution factor and various blocks
and pretreatments. Nothing has changed in our processing or fixation
routines, and the problem is also occurring in blocks that had previously
stained without the heavy background.

I am just about out of ideas, any thoughts would be appreciated.



Mark Daymon




From daemon Fri Feb 2 02:27:52 2001



From: rad0 :      rden25-at-mindspring.com
Date: Fri, 2 Feb 2001 02:21:44 -0600
Subject: SEM samples

Contents Retrieved from Microscopy Listserver Archives
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Is there a place where you can purchase
ready-made examples of things to look
at with an SEM?

I'm just getting started with learning to use an
SEM and I've been thinking that this would
be helpful to have something to look at, that
you already know what it should look like.

Thanks...





From daemon Fri Feb 2 02:50:50 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 02 Feb 2001 00:50:02 -0800
Subject: Re: rotary shadowing, platinum coating

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Hello Heinrich

I am not familiar with technique you described, but let me comment your
message a little bit. First of all, it's not clear to me: are you going to
shadow your sample with Pt and then coat it with carbon or you want to
perform Pt/C shadowing? I assume, you want to try the second one. Pt/C
shadowing gives a less developed granularity because carbon protects Pt
from aggregation/clusterization. The best solution for Pt/C shadowing - to
use electron gun with special Pt/C insert. Many years ago, I did Pt/C
thermal evaporation. For that purpose I did make special carbon rods 1.5
mm dia with hole. I was using hole to insert Pt wire inside the carbon
rod. As long as you are using the same rod you will have highly
reproducible results. Your set-Up seems to me is so tricky and less
reproducible. About thickness: it's impossible to distinguish Pt from C in
the mixed evaporation. Reasonable thickness will be when you just start to
see the difference between exposed and unexposed to the shadowing area of
paper. It will be something brownish, never black (it's too-o-o-o much!).
If you are planning not only shadow your sample but see some details on it,
vacuum in the range of 10-5 torr is not adequate. You, probably have to go
to 2x10-6 torr at least. In general, the cleanness of the system is very
important for shadowing. And the last: I would recommend you will practice
a little bit with latex beads before start real experiment. The latex
suspension is widely available from any EM supply vendors. You may chose
the latex size correlated to your real molecules size. With latex, you will
see perfectly the quality of your shadowing. Doing one-directional
shadowing - you may determine the real angle of shadowing and the quality
of the shadowed metal layer (for the real sample you may switch easily to
the "rotary"). And the very last comment: rotary shadowing comes from DNA
analysis. At that case it was necessary, because of elongated DNA shape
and necessarily to trace the whole thing. For compact globular objects,
there is no advantage for rotary shadowing. One or dual (perpendicular)
shadowing may provide to you more information than rotary. Usually, the
contrast of the rotary shadowed samples is less than for one/dual direction
shadowing (at the same metal thickness). The geometry of the shadow may
give you unique information about 3rd dimension of your object. I suggest
you may try both.

Good luck!

Sergey


At 04:59 PM 1/31/01 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Feb 2 08:24:15 2001



From: tellis2-at-hallmark.com
Date: Fri, 2 Feb 2001 08:23:19 -0600
Subject: SEM images

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John,
We recently disposed of a Philips EM-200 TEM (circa 1963) and 3 others in storage for parts. That left us with 8 high voltage tanks...4 in power cabinets and 4 in scope consoles. Our radiological and environmental management people, responsible for disposing of all hazardous waste on campus, checked the tanks for PCB's and then disposed of the oil and tanks. This is part of their job and there was no charge to us. Do check with your people on campus....I suspect that they also have the means to take care of the problem for you.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907
--------------------------------------


In order to meet federal requirements, the oil should be tested for PCB
content. A result of less than 50 mg/kg is considered non-PCB. A
concentration that 50 mg/kg to 499 mg/kg is considered to be contaminated
with PCBs. And a concentration of 500 mg/kg or greater is considered to be
PCB. There is an entire code in the Federal Register that deals with the
proper way to dispose of these types of oil. If the concentration is found
to be 50 mg/kg or greater, the oil is considered to be Hazardous Waste, and
falls within the RCRA standard. The following URL will take you to the
EPA's PCB Home Page where your friend will find several links to answer his
questions.

http://www.epa.gov/opptintr/pcb/

Hope this helps.

Regards,
Beth Bray

Elizabeth P. Bray
Plant Chemist, Central Laboratory
South Carolina Electric and Gas Co.
2102 N. Lake Dr.
Columbia, SC 29212

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 31, 2001 5:33 PM


Walter McCrone has published a Particle Atlas that has lots of SEM
images and their EDX spectra and there are also light images of the same
materials, its been very useful reference for me. Its on a CD called PAE2
Particle Atlas. You can then collect the same materials and see what they
look like on your systems or McCrone also sell collection of materials you
could buy. I have collected samples of materials associated with the paper,
printing/plating industry and EHS (office dusts) since that is what I look
at most of the time .
I have no interest in McCrone.
I just use their stuff.
Terry Ellis
Hallmark Cards Inc.
tellis2-at-hallmark.com



From daemon Fri Feb 2 11:36:11 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 02 Feb 01 09:40:02 -0800
Subject: RE: LM. CD 34 help

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Reply to: RE: LM. CD 34 help

Gramp Skin Pathology wrote:
} I have used CD 34 QBend10 clone, to demonstrate endothelial cells for
} several years. It gave a clean picture with no problems.
}
} 3 months ago it started to pick up collagen, elastic and general connective
} tissue at such a high intensity that the technique became almost unusable.
}
} I have changed the primary antibody and every other reagent involved in the
} technique. I have also tried changing the dilution factor and various blocks
} and pretreatments. Nothing has changed in our processing or fixation
} routines, and the problem is also occurring in blocks that had previously
} stained without the heavy background.
}
} I am just about out of ideas, any thoughts would be appreciated.

This problem will have a logical explanation for why your labeling method has stopped working. It will be based either on something you changed (so look VERY carefully at the protocol actually being used) or something that has happened to either the reagents or the samples.
As the problem occurs with samples that previously worked well, then it seems that specimen preparation can be ruled out as a source. Therefore you must look carefully at the reagents and the treatments you perform on the sections.

If the fixation protocols, the tissues being used and the labeling protocols are all the same (ie there have been NO changes), then the question to ask is: how do you store and handle your antibodies?

If there is no doubt that the antibody storage is not the problem, then the next question is: what is the blocking agent being used and has that been changed recently?


Regards,

Paul Webster




Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm






Gramp Skin Pathology wrote:

}

}
}
}
} Mark Daymon
}
}
}
}
}
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From daemon Fri Feb 2 12:54:31 2001



From: A.G.Cullis :      A.G.Cullis-at-sheffield.ac.uk
Date: Fri, 2 Feb 2001 20:10:44 -0000
Subject: Microscopy of Semiconducting Materials Conference

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Message-ID: {000c01c08d48$ed79ad30$8a68fea9-at-Sony}


Thanks to all...

I think I'll just use the light bulb filament to see if everything works.

But, I'm interested in using this SEM to view fungi.

I'd like to start a small catalogue of these things.

This is probably on the intenet already?





----- Original Message -----
} From: "White, Woody N." {nwwhite-at-mcdermott.com}
To: "'rad0'" {rden25-at-mindspring.com}
Sent: Friday, February 02, 2001 7:39 AM


Royal Microscopical Society

12th International Conference on

MICROSCOPY OF SEMICONDUCTING MATERIALS

25-29 March 2001, University of Oxford, UK

********************************
Final Announcement
********************************

This international conference will focus on the latest developments
in the study of the structural and electrical properties of
semiconductors by the application of transmission and scanning
electron microscopy, scanning probe microscopy and X-ray
techniques.

The state-of-the-art in all important subject areas will be
addressed, including the characterisation of bulk and thin film
as-grown materials, the study of lattice defect and impurity
behaviour and the investigation of advanced semiconductor
processing procedures.

Special conference sessions will concentrate on recent
developments in high-resolution imaging and analytical electron
microscopy, advances in SEM and SPM applications, the
characteristics of epitaxial layers (including III-V nitrides),
quantum wells, wires and dots, the effects of device processing
treatments (including, especially, advanced silicon technology) and
metal-semiconductor contacts and silicides. Prominent invited
speakers will introduce each topic area.

The full conference programme, together with registration
information, is now available on the web site:

http://www.rms.org.uk/currentevents2.htm#MSMXII

Please contact the Royal Microscopical Society or the under-
signed for any additional information.

Tony Cullis
MSMXII Co-Chairman

****************
Professor Anthony G Cullis
Head of Semiconductor Materials & Devices Group
Dept of Electronic & Electrical Engineering
University of Sheffield
Mappin Street
Sheffield
S1 3JD, UK

Tel: +44-114-222-5407
Fax: +44-114-272-6391
E-mail: a.g.cullis-at-sheffield.ac.uk


From daemon Fri Feb 2 15:26:27 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Fri, 02 Feb 2001 16:17:32 -0500
Subject: Interamerican Congress

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I would like to encourage people to attend the forthcoming Interamerican
Microscopy Congress. Previous meetings have been great fun and
scientifically rewarding. Details follow.
Alwyn Eades

VI Interamerican Congress on Electron Microscopy

October 7 - 11 2001
Hotel Emporio
Veracruz, México

The Interamerican Congress on Electron Microscopy was previously held in
Merida Venezuela, 1992; Cancún, México, 1993; Cancún, México, 1995;
Guayaquil Ecuador, 1997 and Margarita Island Venezuela, 1999
This is the official congress of Committee of Inter-American Societies
for Electron Microscopy (CIASEM) and is a forum for microscopy in The
Americas.
The Congress will have plenary talks, invited talks and a poster
session.
Proceedings will be published.

Congress Site
The congress will take place at Hotel Emporio, which has a fine location
in the port of Veracruz: It is in the heart of the historic center.
The tourist attractions of the port of Veracruz and surroundings range
from a rich nightlife to sites of unmatched natural beauty. It was the
home of the Olmeca culture and is where Hernán Cortés disembarked.
There are wonderful beaches. The well-preserved archeological areas,
vast tropical forests, and colonial cities make Veracruz a fascinating
place to visit.

Conference Topics:

Materials Science
Electron Microcopy of magnetic materials, fracture mechanics, thin films
and semiconductors, materials, computer simulation, sol-gel materials,
polymers, ceramics, glasses, renewable energy materials, recycling
materials, surfaces characterization, corrosion, composites, and general
topics in material science.

Biological Sciences
In situ hybridization, immunolocalization, scanning probe microscopy in
biology, pathology, microscopy and cell biology, neurobiology, and
general
topics in biological sciences

Short Courses and Labs:
Sample preparation
Scanning microscopy
TEM and High Resolution
Digital and Image Processing
Advances in remote control electron microscopy

Exhibition
Electron microscopes, sample preparation and related products will be
displayed during the congress. Exhibitors in fields related to new
microscopies and optical microscopies are encouraged to participate.

Call for papers
Abstracts must be contained in exactly 2 pages. The first page will
include
only text, including title, authors, affiliation, main body of the work
and
references. The second page will include text, tables and figures. Use a
word processor, with high quality printer, TIMES NEW ROMAN type letter
in
12 points, and a single space line.
Abstracts will be published in Acta Microscopica

Deadline to send abstracts is July 15, 2001.

English will be the official language of the Congress. Translation
facilities will not be available.

Hotel Reservation
Special rates are available at the Hotel Emporio for Congress attendees.
For reservations contact the hotel directly mentioning the congress.
Single rooms are US $98, Doubles $108.50, extra people $17. Suites
are $116 (single) and $138 (double).

Hotel Emporio
Paseo del Malecón s/n, Col. Faros C.P. 91700
Tel. (5229) 32 00 20 Fax (5229) 31 22 61
Email: emporio-at-ver.megared.net.mx
Reservations: Fax. (5229) 31 66 24
and 01 800 29 520 00

Congress Registration fees

Before august 31, 2001 $ 300.00 USD
Students: $ 100.00 USD
After august 31, 2001 $ 325.00 USD
Students: $ 125.00 USD
Courses
Before august 31, 2001 $ 60.00 USD
After august 31, 2001 $ 80.00 USD

Chairman:
Miguel José Yacamán (yacaman-at-che.utexas.edu)

Informations and Registration
Dr. José Reyes-Gasga
Instituto de Física, UNAM
Apartado Postal 20-364, 01000
México D.F., México
Phone: (525) 622-5083 Fax. (525) 622-5008
Email: jreyes-at-fenix.ifisicacu.unam.mx


From daemon Fri Feb 2 17:30:05 2001



From: Vachik Hacopian :      vhacopian-at-wellesley.edu
Date: Fri, 2 Feb 2001 18:25:08 -0500
Subject: SEM Lab @ Harvard MCZ

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could anyone provide me with the telephone number of the Laboratory of
Scanning Electron Microscopy at the Harvard Museum of Comparative Zoology?
Many thanks in advance.

Vachik Hacopian




From daemon Fri Feb 2 17:46:15 2001



From: Stephen Skirius :      Stephen_Skirius-at-bkitech.com
Date: Fri, 2 Feb 2001 17:46:04 -0600
Subject: AO microtome knife sharpener

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have a model 903/904 AO automatic knife sharpener. I am missing the
redressing bridge for reconditioning the glass plates. Does anyone have one
they would like to get rid of?

Steve




From daemon Sat Feb 3 05:07:24 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 03 Feb 2001 03:00:35 -0800
Subject: Re: black & white print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kreonite is pretty much a notable industry standard for
paper and emulsion autoprocessing. These are not low
cost units, however. Rebuilt ones can be found at places
like:

http://www.dunningphoto.com/rebuilt.html

You can also search for other sources using the key
model numbers which suit your needs.

gary g.


At 01:33 PM 1/30/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Feb 3 05:23:16 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 03 Feb 2001 03:19:23 -0800
Subject: Re: SEM and LM image capture on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Media Cybernetics Image Pro-Plus. Then add an NTSC
frame grabber card which it supports. The Matrox and
National Instruments cards are very good. with this
complement, you can average, integrate, Kalman and
do pixel mapping as well as image archiving from a
standard TV video source.

http://www.mediacy.com

gary g.


At 07:03 AM 1/29/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Feb 3 09:47:12 2001



From: carlos-e-chavez-at-uiowa.edu
Date: Sat, 3 Feb 2001 09:34:29 -0600
Subject: Ask-A-Microscopist: Microscopy of Dental Interfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: carlos-e-chavez-at-uiowa.edu
Name: carlos e chavez,dds
School: University of Iowa College of Dentistry
State: Iowa

I am an graduate student currently enrolled in a Master's program in
Operative Dentistry at University of Iowa, College of Dentistry. My
research interest involves the evaluation of interfaces between composite
resins used in dentistry and a noble (Au 51.5%, Pd 38%) and base metal
alloys (NiCrBe). I have mounted a metal disc of 10mm. diameterX 2mm thick
(Noble or base alloy) in Epoxy resin and followed the protocol used in
dental research for metal preparation (280, 400,and 600 grit Si Carbide
paper under running water, etc). Then, using a brush I painted on the metal
surface delimited by a tape with a 6 mm diameter hole using a resin opaquer
paste of 0.5 mm thick. Then using a mold of 2.38 mm diameter a applied a
composite resin and light cured to harden it.Finally, I cleaned the excess
opaque in the periphery. My question is how do you prepare this three
element interface for observation under the SEM or optical microscope?
How would I make the cuts so I do not disturb the bond between the three
elements and examine at these interfaces under the microscope accurately?.
I hope this is clear.
Thank you very much.
Carlos E. Chavez, DDS
University of Iowa College of Dentistry








---------------------------------------------------------------------------




From daemon Sat Feb 3 09:47:12 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 03 Feb 2001 09:42:54 -0500
Subject: Pt wire diameter + Pt/C evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

The "main" way Pt/C I thought was being evaporated today is the "bead on
carbon" method, that is, where the wire is coiled up, around a pin of
diameter slightly larger than the diameter of the neck on the carbon rod,
and then, with some help from some good Style #5 tweezers, it is pulled onto
the sharpened neck of the carbon rod. The bell jar is lowered over the
carbon rods and holders, and wearing the appropriate eye protection, the
power is slowly turned up, slowly heating the carbon rods and wound Pt wire.
At some point the miracle happens: The Pt melts, and surface tension
effects cause it to form (in an instant) a nice little "bead" (it looks like
the textbook "sessile drop") firmly attached to the carbon rod once the
heating is stopped. After cooling down, the rod with the drop is rotated
around so that it is facing where the samples will be, the bell jar is then
loaded, pumped down and evaporation of Pt/C can be done simultaneously this
way.

Indeed I don't think it is possible to evaporate Pt wire **without** the
formation of the sessile drop, therefore, how the wire is originally spread
out (on the rod neck) does not matter!

A note not related to the original question: The sharpened "neck" should be
sharpened to a diameter not more than 3/16" (4.75 mm). The method will not
work as well if the neck diameter is larger than this. If you are
sharpening your own rods, make sure you are using rods of higher rather than
lower density, otherwise the rod will not have the mechanical properties
needed to sharpen down to this diameter.

I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil
) wire since a previous posting seemed to attribute to it something special
(relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe
that to be so, that for the practice of the sessile drop method, either
diameter wire would "bead up" just as quickly and easily, and the only
difference between the two diameters would be one of cost, since the cost to
draw the same mass in 4 ml is obviously going to be higher than that to draw
8 mil. Actually some years ago we came into some 10 mil Pt wire, and we
found it could be used just as easily to make the sessile drop.

Furthermore since either diameter wire could be used to create a drop of
equal size, the actual evaporation time would be independent of the diameter
of the starting wire.

In the end, we came upon the novel conclusion that I would make a posting
and subject my advice to the most stringent (microscopy) peer review panel
in the world, namely the listserver. Am I not correct, in that the end
result will be the same irrespective of whether 4 mil or 8 mil wire is used?

Chuck

Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire
that is used for evaporation in electron microscopy applications.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Feb 3 10:26:31 2001



From: NPGSlithography-at-aol.com
Date: Sat, 3 Feb 2001 11:19:33 EST
Subject: Re: Lithography information needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A good place to start would be:

Handbook of Microlithography, Micromachining, and Microfabrication :
Microlithography
by P. Rai-Choudhury (Editor)
Hardcover Vol 001 (June 1997)
SPIE Press; ISBN: 0819423785

This is a very thorough book which covers optical lithography, electron beam
lithography, and x-ray lithography, as well as other related topics. The
chapter on e-beam lithography is on the web at
"http://www.cnf.cornell.edu/spiebook/toc.htm".

A list of other books that may be useful can be found at
"www.jcnabity.com/booklist.htm".

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com

In a message dated 2/1/2001 11:25:38 PM Mountain Standard Time,
u980013-at-giki.edu.pk writes:

} Hi,
} I am a student doing a Research paper on the subject ofLithography and
} Semiconductor Fabrication
} I need information on the subject of=20
} Lithography....Its types specially Photolithography,
} Electron beam Lithograaphy, UV lithography and Xray lithography
} Plz help me...if u have any articleds plz send them to me...
} or tell me of WEB Resources that can help..
} plz reply soon
} u980013-at-giki.edu.pk
}



From daemon Sat Feb 3 15:10:32 2001



From: Rassad-at-students.miami.edu () (by way of Nestor J. Zaluzec)
Date: Sat, 3 Feb 2001 15:07:23 -0600
Subject: Ask-A-Microscopist: see a protein in an sem?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



---------------------------------------------------------------------------

Email: Rassad-at-students.miami.edu
Name: Rizwan Assad
School: University of Miami


Question: I would like to know if I would be able to see a protein that
weighs about 100 kilo daltons under an SEM. If so, where can I find
resourses for the preparation of the specimen?

---------------------------------------------------------------------------




From daemon Sat Feb 3 15:10:32 2001



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Sat, 03 Feb 2001 15:02:28 -0500
Subject: Vibratome manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I ran across an original manual for an Oxford Vibratome--Model G-Catalog
no. 501 and 502. Since we no longer have the vibratome, we don't need the
manual. Does anyone want it?

Bob
Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
tele: (920) 424-3404
fax: (920) 424-1101
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html


From daemon Sat Feb 3 15:13:24 2001



From: Seth Grotelueschen :      sethg-at-paxit.com
Date: Sat, 3 Feb 2001 11:02:36 -0600
Subject: RE: SEM and LM image capture on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

You can also look at www.integraltech.com for very reliable image capture
cards. They have 1000's installed (we have sold that many alone). They
have AGP and PCI versions. Also can handle signals from RGB to NTSC
Composite.

As for software -- there is a product called A4i that is pretty good (not
ours) and www.paxit.com (ours) that handle all of the archiving, measuring,
Excel interface, report generation and image analysis functions you are most
likely desiring. We have thousands of systems in stalled and many in
semicon mnfg and QA. Both of these products have network versions that
allow less expensive licenses for your work outside the fab.

Good luck,

Seth G.



-----Original Message-----



From: Seth Grotelueschen :      sethg-at-paxit.com
Date: Sat, 3 Feb 2001 11:02:36 -0600
Subject: Re: SEM and LM image capture on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Media Cybernetics Image Pro-Plus. Then add an NTSC
frame grabber card which it supports. The Matrox and
National Instruments cards are very good. with this
complement, you can average, integrate, Kalman and
do pixel mapping as well as image archiving from a
standard TV video source.

http://www.mediacy.com

gary g.


At 07:03 AM 1/29/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Sun Feb 4 09:56:09 2001



From: Bert Luttmer :      mylab-at-telekabel.nl
Date: Sun, 04 Feb 2001 16:38:20 +0100
Subject: LM rapid & accurately relocating cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ladies and gentlemen,

CellFinder Microscope Slides are described in detail in website
http://home.talkline.nl/mylab/

A durable coded micropattern on the slide surface enables cell relocation
with an accuracy of about 5 microns.

Pattern masks are made with latest IC manufacturing technology in a large
semiconductor laboratory.

Slides can be sterilized and re-used during years without loss of pattern
quality.

General field of applicatons: genetics, geology, hydrology, algae
reasearch, plant systematics.

In a focal plane of optical systems for aerospace photography to project
pattern codes on the image of film or video-chip.

I am available for any specific question regarding special applications of
CF-slides and will answer you to the best of my knowledge.


Ing Bert Luttmer
Microlab
CellFinder developer since 1973
Arnhem - The Netherlands



From daemon Sun Feb 4 12:22:36 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 4 Feb 2001 11:11:29 -0700
Subject: RE: SEM and LM image capture on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

there are definitely many cards out there to acquire standard TV signals
from any source that adheres to that standard. These standards were
developed decades ago for TV cameras, and my personal opinion is, that they
are good for moving images (as on TV), but they lack resolution and
definition for still images (LM and SEM). However, since they cards are
usually not very expensive, and some of them do offer integration
capabilities (normally frame integration), it might be worth a shot. Also
the microscopes themselves might be offering integration capabilities. You
are limited to a resolution of 640x480 (NTSC) or 758x576 (PAL).

For better resolution you might want to look at other options as well. For
SEMs there are interfaces available (such as our ADDA II, but there are
other manufacturers also), normally available as "passive" or "active"
systems. For LM, of course, there are digital cameras with better resolution
and bit-depth (Video only carries about 8 bits of information, if you're
lucky).

Look for systems that are open for upward expansion to keep your options
open. Contact me offline if you are interested in getting more information
about the way we do that.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

At 07:03 AM 1/29/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Mon Feb 5 00:05:33 2001



From: Volfova Petra :      volfova-at-chelin.chtf.stuba.sk
Date: Mon, 5 Feb 2001 00:00:46 -0600
Subject: TEM-Preparation of dispersion samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I would like to request of preparation samples from
poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size
for measurements by JEOL type of transmission electron microscope.
We have got some problems with dilution of latexes and with time of
staining of carbon, too. We would like to confirm a core/shell
particles morphology of samples prepared by two step seeded emulsion
polymerization. We have got some problems with magnification and contrast
expansion of core/shell particles,too.
Thank you very much for your help.

Petra Volfova, PhD. student of Department of plastics and rubber,
Slovak Technical University,Bratislava,Slovak Republic.




From daemon Mon Feb 5 00:08:09 2001



From: Damian :      dneuberger-at-mindspring.com
Date: Mon, 5 Feb 2001 00:07:32 -0600
Subject: Midwest Microscopy&Microanalysis Mtg Feb. 13, 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



MIDWEST MICROSCOPY AND MICROANALYSIS SOCIETY, INC. (MMMS)
AFFILIATE OF THE MICROSCOPY SOCIETY OF AMERICA

MEETING ANNOUNCEMENT

JOINT MEETING WITH THE SOCIETY FOR APPLIED SPECTROSCOPY
Tuesday, Feb. 13, 2001

At Unilever HPC USA, located at 3100 Golf Road, Rolling Meadows, IL. See
directions for more details.

Social Hour: 5:30 pm
Dinner: 6:00 pm
Speaker: 7:00 pm
_________________________________________________________________
The Infrared Microprobe in Production and Research
By Koichi Nishikida

Abstract
Unlike the visible microscope and electron microscope, which respectively
use visible light and electrons to magnify the sample, the infrared
microscope does not utilize infrared wavelengths to magnify the sample
image. Instead, a visible microscope is modified, so that a magnified
sample image is observed and, at the same time, an infrared beam is passed
through the microscope to obtain an infrared spectrum of the sample.
Therefore, the "IR microscope" should be better named the "IR microprobe".

Applications of the IR-microprobe cover applications from forensic
} analysis
to defect analysis, reverse engineering in the production industry, and
medical diagnostic research.

In this talk, I will show how the IR-microprobe has contributed to quality
improvements in the production industry, as well as recent research in
medical applications. Briefly, I will address the evolution of this
technique over the past five decades.
*********************************************************************
Please make your dinner reservations for the upcoming meeting by calling
(847)734-3712. Leave your name, company affiliation and the number of
reservations. Please call by noon on February 9th, so that proper
arrangements can be made.
Dinner Cost:
M3S Members: $25
M3S Students: $10
Nonmembers: $30


Directions to Unilever, Rolling Meadows, Illinois

From the Chicago Area:
Kennedy Expressway to I-90 West (Rockford);
I-90 West to Arlington Heights Rd.;
North to Golf Rd. (2nd traffic light);
Turn left (West) onto Golf Rd. for approximately 2 miles.
The Unilever R&D facility is on the right, just before Hwy. 53, across from
the forest preserve. Follow the entrance road all the way to the last
parking lot (North lot) and enter the building through the far glass doors.


From I-290 Eisenhower Expressway:
Stay on I-290 towards Rockford;
Exit on Golf/Higgins Rds.;
Continue North to Golf Rd.;
Turn east on Golf Rd.
Unilever is1/4 mile left on Golf Rd., across from the forest preserve.
Follow the entrance road all the way to the last parking lot (North lot)
and enter the building through the far glass doors.


From the Northern Suburbs (Route 53 South):
Exit Woodfield Drive/Golf Rd. (58);
Continue to first light;
Turn left at first light;
Continue to Golf Rd (58);
Turn right (East) on Golf Rd.;
Unilever is1/4 mile left on Golf Rd., across from the forest preserve.
Follow the entrance road all the way to the last parking lot (North lot)
and enter the building through the far glass doors.

If you have any questions about the meeting, please direct your inquiries
to the phone number below. For questions about MMMS, please contact:

Dr. Damian Neuberger
Senior Research Scientist
Baxter Healthcare, Corp.
(847) 270-5888
damian_neuberger-at-baxter.com







From daemon Mon Feb 5 00:09:04 2001



From: Kevin McCleary :      kmccleary54-at-hotmail.com
Date: Mon, 5 Feb 2001 00:08:37 -0600
Subject: prepared slides question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Email: kmccleary54-at-hotmail.com
} Name: Kevin McCleary
} School: South Peace Secondary School
} State: British Columbia, Canada
}
} Question: I am anticipating the purchase of prepared slides for an
} introduction to cells in my grade ten science classes.Ý Will chromosomes be
} easily viewed in smears of Drosophila (advertized as giant chromosomes)?
} If not, could you suggest alternate subjects.Ý Our light microscopes are of
} standard high school quality.
}
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.




From daemon Mon Feb 5 06:22:33 2001



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Mon, 5 Feb 2001 08:25:47 -0600
Subject: Sand Request for Microscopy Education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck's reply without the customary inclusion of the previous contribution is
difficult to understand. I have now added my previous contribution and the
original request below.
The hanging drop method results largely in Pt evaporation followed by carbon.
The method also requires breaking the vacuum and is therefore more trouble. As
Sergey Ryazantsev in his thoughtful contribution explained (I erased that
because there was no need to reply), its the simultaneous nature of Pt/C which
yields the finer grain structure. Sergey also explained that the other means of
achieving such fine grain is electron beam evaporation, but that requires
special equipment.
I explained in my previous contribution why the thinner wire is required.
30 years ago I used many meters annually of the 0.1mm Pt for freeze etching and
Kleinschmitt/ DNA rotary shadowing.
Nothing for it Chuck: you will need to stock 0.1mm Pt wire like all other major
suppliers.
Don't be overly concerned, very few people use these techniques now; in fact
you could purchase your minor requirements from us!
Disclaimer: its too obvious, we sell small quantities of 0.1mm Pt.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Sunday, February 04, 2001 12:43 AM, Garber, Charles A.
[SMTP:cgarber-at-2spi.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} The "main" way Pt/C I thought was being evaporated today is the "bead on
} carbon" method, that is, where the wire is coiled up, around a pin of
} diameter slightly larger than the diameter of the neck on the carbon rod,
} and then, with some help from some good Style #5 tweezers, it is pulled onto
} the sharpened neck of the carbon rod. The bell jar is lowered over the
} carbon rods and holders, and wearing the appropriate eye protection, the
} power is slowly turned up, slowly heating the carbon rods and wound Pt wire.
} At some point the miracle happens: The Pt melts, and surface tension
} effects cause it to form (in an instant) a nice little "bead" (it looks like
} the textbook "sessile drop") firmly attached to the carbon rod once the
} heating is stopped. After cooling down, the rod with the drop is rotated
} around so that it is facing where the samples will be, the bell jar is then
} loaded, pumped down and evaporation of Pt/C can be done simultaneously this
} way.
}
} Indeed I don't think it is possible to evaporate Pt wire **without** the
} formation of the sessile drop, therefore, how the wire is originally spread
} out (on the rod neck) does not matter!
}
} A note not related to the original question: The sharpened "neck" should be
} sharpened to a diameter not more than 3/16" (4.75 mm). The method will not
} work as well if the neck diameter is larger than this. If you are
} sharpening your own rods, make sure you are using rods of higher rather than
} lower density, otherwise the rod will not have the mechanical properties
} needed to sharpen down to this diameter.
}
} I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil
} ) wire since a previous posting seemed to attribute to it something special
} (relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe
} that to be so, that for the practice of the sessile drop method, either
} diameter wire would "bead up" just as quickly and easily, and the only
} difference between the two diameters would be one of cost, since the cost to
} draw the same mass in 4 ml is obviously going to be higher than that to draw
} 8 mil. Actually some years ago we came into some 10 mil Pt wire, and we
} found it could be used just as easily to make the sessile drop.
}
} Furthermore since either diameter wire could be used to create a drop of
} equal size, the actual evaporation time would be independent of the diameter
} of the starting wire.
}
} In the end, we came upon the novel conclusion that I would make a posting
} and subject my advice to the most stringent (microscopy) peer review panel
} in the world, namely the listserver. Am I not correct, in that the end
} result will be the same irrespective of whether 4 mil or 8 mil wire is used?
}
} Chuck
}
} Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire
} that is used for evaporation in electron microscopy applications.
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================

} From: Jim at ProSciTech [SMTP:jim-at-proscitech.com]
Sent: Friday, February 02, 2001 9:59 AM
To: 'Heinrich Matthies'; Microscopy-at-sparc5.microscopy.com


Folks,

Past sand donations from listserver subscribers helped start the MSA sand
collection. This collection is freely given to educators to use with
Microscopic Explorations and other educational programs. Recently, we
advertised this resource to educators and the response has been
overwhelming. Consequently much of the sand collection has been given away
and the collection is in need of restoration. As you can guess, the most
popular sands are from locations outside the United States.

So I am asking for donations from listserver subscribers, especially those
outside the United States. At one point we had sand from every continent,
but that is not the case any more. Please help us rebuild the MSA sand
collection. Double bag your sand in sealable bags (Ziplock baggies work
great) and mail your donations to:

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com

A list of the current collection can be found at:

http://microscopy.com/ProjectMicro/SandCollection.html

Thanks,
Joe Neilly


From daemon Mon Feb 5 09:20:11 2001



From: Lesley Graham :      LGRAHAM-at-utsa.edu
Date: Mon, 5 Feb 2001 09:16:20 -0600
Subject: unsuscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon Feb 5 10:33:17 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 5 Feb 2001 10:23:12 -0600
Subject: RE: Ask-A-Microscopist: Microscopy of Dental Interfaces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Carlos,

} From your description of the specimen preparation it seems you have
prepared a sample for some type of mechanical test (shear bond test?)
If you need just observe an interface you can prepare samples for this
purpose. Do not embed you disks in Epoxy. Treat all disk surface with
with resin and composite. On low speed diamond saw cut disks in halves.
Then embed them in Epoxy and polish with diamond pastes. All adhesives
and composites should withstand this treatment easily. The most difficult
part is polishing - glass particles of a composite filling could produce
a lot of scratches, so treat you sections gently.

Good luck,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "carlos-e-chavez-at-uiowa.edu"-at-sparc5.microscopy.com
} [mailto:"carlos-e-chavez-at-uiowa.edu"-at-sparc5.microscopy.com]
} Sent: Saturday, February 03, 2001 9:34 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Microscopy of Dental Interfaces
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} Email: carlos-e-chavez-at-uiowa.edu
} Name: carlos e chavez,dds
} School: University of Iowa College of Dentistry
} State: Iowa
}
} I am an graduate student currently enrolled in a Master's program in
} Operative Dentistry at University of Iowa, College of Dentistry. My
} research interest involves the evaluation of interfaces
} between composite
} resins used in dentistry and a noble (Au 51.5%, Pd 38%) and base metal
} alloys (NiCrBe). I have mounted a metal disc of 10mm.
} diameterX 2mm thick
} (Noble or base alloy) in Epoxy resin and followed the protocol used in
} dental research for metal preparation (280, 400,and 600 grit
} Si Carbide
} paper under running water, etc). Then, using a brush I
} painted on the metal
} surface delimited by a tape with a 6 mm diameter hole using a
} resin opaquer
} paste of 0.5 mm thick. Then using a mold of 2.38 mm diameter
} a applied a
} composite resin and light cured to harden it.Finally, I
} cleaned the excess
} opaque in the periphery. My question is how do you prepare this three
} element interface for observation under the SEM or optical microscope?
} How would I make the cuts so I do not disturb the bond
} between the three
} elements and examine at these interfaces under the microscope
} accurately?.
} I hope this is clear.
} Thank you very much.
} Carlos E. Chavez, DDS
} University of Iowa College of Dentistry
}
}
}
}
}
}
}
}
} --------------------------------------------------------------
} -------------
}
}
}


From daemon Mon Feb 5 12:06:54 2001



From: Bradley, John :      john.bradley-at-mse.gatech.edu
Date: Mon, 5 Feb 2001 12:12:48 -0500
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Mon Feb 5 14:28:05 2001



From: Judy Trogadis :      judy-at-uhnres.utoronto.ca
Date: Mon, 05 Feb 2001 15:21:36 -0800
Subject: data acquisition software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are about to buy a Hamamatsu Orca CCD camera to
capture images, viewed with both
fluorescent and transmitted light. This seems to be a
popular choice of camera among microscopists. We would
like to know which are the different (favorite)
software packages that people are using to collect,
display and analyze the data captured by an Orca
camera.

Thank you.


Judy Trogadis
Vision Science Research Program
Toronto Western Research Institute
399 Bathurst St.
Toronto, ON M5T 2S8
ph: 416-603-5088
fax: 416-603-5126
email:judy-at-uhnres.utoronto.ca




From daemon Mon Feb 5 15:58:24 2001



From: Richard Gordon :      gordonr-at-Ms.UManitoba.CA
Date: Mon, 5 Feb 2001 15:39:06 -0600
Subject: Finding Reprint Authors' E-Mail Addresses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric Cowdrey and I have put together a web page that makes it
relatively easy to find the e-mail addresses of academic colleagues,
for the purpose of requesting reprints:

Finding Reprint Authors' E-Mail Addresses
http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html

There are no a d v e r t i s e m e n t s or costs. If you use it,
please let us know of omissions, corrections, or improvements you'd
like, and forward it to your colleagues.
--

Dr. Richard Gordon, Radiology, University of Manitoba, HSC Rm. GA216,
820 Sherbrook St.
Winnipeg R3A 1R9 Canada
Adjunct: Electrical & Computer Engineering, Exec Member: CSTB, CARRF, IEEE-EMBS
phone:(204)789-3828, fax:(204)787-2080, e-mail: GordonR-at-ms.umanitoba.ca
New book: The Hierarchical Genome & Differentiation Waves: Novel
Unification of Development, Genetics & Evolution:
http://www.wspc.com.sg/books/lifesci/2755.html
Finding Reprint Authors' E-Mail Addresses:
http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html


From daemon Mon Feb 5 19:06:18 2001



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 5 Feb 2001 18:51:49 -0600
Subject: SECOND ANNOUNCEMENT: 6th Live-cell Course at UBC: June 18-28

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SECOND ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March 1!!

Hello all,

The faculty for the 2000 UBC 3D Live-cell Microscopy Course is now almost set .

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted Inoué Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Michael Weis Agriculture Canada


Basic info is below but you can get the entire brochure, including
links to faculty home pages, at

http://www.cs.ubc.ca/spider/ladic/course/brochure.htm

Cheers,

Jim P.

Sixth Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells
June 18 - 28, 2001


Fifth, Post-course Workshop on

3D Image Processing,
June 30 - July 3, 2001


Organized by Prof. James Pawley,
(University of Wisconsin-Madison)

in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada



DATES

Applications must be received by March 1, 2001
Deposit due April 15, 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001
3D Image Processing Wksp Sat. June 30 - Monday, July 2


APPLICATIONS DUE BY MARCH 1, 2001


More information at :
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or

REGARDING COURSE ORGANIZATION:

Contact:
Prof. James B. Pawley,
JBPAWLEY-at-FACSTAFF.WISC.EDU

REGARDING APPLICATIONS

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4


THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary improvement
in our ability to view living cells. To help convert this promise to
reality for a wider selection of biological scientists, an intensive
eleven-day residential course concentrating on all aspects of 3D Microscopy
of Living Cells will be held at the University of British Columbia, in June
of 2001. The course includes 4 days on 2D techniques, 6 days of 3D
techniques and a summary presentation. It covers basic microscopy to the
highest level confocal microscopy. (A half-day Pre-course is offered for
any who may need to brush up on basic optics!).

Topics include:
o Quantitative 2D light microscopy
o How to keep your cells alive
o 3D imaging in confocal microscopy
o Widefield/deconvolution techniques
o Two-photon excitation microscopy
o Fluorescent and backscattered light signals
o Dye design, characteristics and use
o Pixelation: The Nyquist Criterion
o Lasers and laser tweezers
o Objectives and aberrations
o Scanning-systems: AODs and mirrors
o Optimal pinhole size/photon efficiency
o Detectors: operation and performance
o Video-rate confocal imaging
o Measuring ion concentrations
o Display and measurement of 3D data

Morning lecture/demonstrations lead to hands-on laboratory exercises each
afternoon that will utilize most of the commercial instruments currently
available for 3D microscopic imaging. Students will work in groups of 3 or
4 throughout the discussion and laboratory sessions, and may complete a
live-cell 3D study on their own specimens. In the first five years, over
130 students from 23 countries have attended. Last year, 11 separate, 3D
microscopical workstations were available for student use under the
supervision of a 17-member international faculty. We expect to have even
more workstations in 2001. Including manufacturers representatives, the
teacher/ student ratio will be almost 2:1.

INTERNATIONAL FACULTY

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Elaine Humphrey University of British Columbia
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Glen MacDonald University of Washington
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Michael Weis Agriculture Canada

TUITION

Course tuition is $2,150 US and includes lunches. On receipt of 50%
deposit, students will receive preliminary group assignments and the
textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The
tuition fee includes the textbook, a binder of handouts, and tickets for
the Opening Reception, the Manufacturer's Reception and the Beach Party.
Accommodations and meals other than lunch are not included in the tuition
fee. The Pre-course is $100 US.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrollment is limited to about 24 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts on
request to read before the course begins and are encouraged to take the
Pre-course on the afternoon of June 18.


Application forms, and other course information from this and past years,
can be downloaded from the WWW site at:

http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4
Phone: 1-604.822-3354
FAX: 1-604.265-5315
Email: ech-at-unixg.ubc.ca.


Application deadlines:

Application forms are due by March 1, 2000!
Deadlines are early to facilitate setting up groups. Successful applicants
will be notified by April 1, and a deposit of 50% must be received by April
15, 2000 to reserve your position. In general, deposit refunds are only be
possible if your position can be filled from the waiting list. The
remainder of the fees is due at registration.

DATES

Applications must be received by March 1, 2001
Deposit due April 15 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001


TEACHING PHILOSOPHY

As befits teaching in an area at the boundary of "what is now known,"
lecturers have been chosen based on their expertise as scientists working
in the field rather than because they all agree. They are encouraged more
to be provocative than to be prosaic. Students should expect discussion in
areas where differences of opinion exist.

Prior to the course, students will be organized into groups and encouraged
to communicate by email/phone, about the "Living-cell" group projects that
they will pursue during the course and that will be presented to the class
on the last day of the course. It has been found that group interactions
make best use of students' prior experience and can be very effective in
teaching the practical skills covered in a hands-on course of this type.

ARRANGEMENTS FOR LIVE SAMPLES

Students must contact Dr. Elaine Humphrey to make necessary arrangements
for the transport and maintenance of cell lines etc. needed for their
projects. Organisms linked in any way with human disease are not permitted
because of safety considerations. Transport and customs arrangements for
living specimens are entirely the responsibility of the student.
Students also attending the 3D Image Processing Course, may be able to
analyze, process and display some of the 3D data collected from their
specimens


********************************************************************************

Fourth Annual

3D MAGE PROCESSING WORKSHOP

The workshop will cover 3D image processing for measurement and display.
Enrollment is limited to those attending the 3D Microscopy course.
Tuition : $850 US (lunches and snacks incl.)

WHO SHOULD ATTEND?

The course is designed for biologists working with multidimensional and
possibly multicolor microscopical data sets. Getting the data is only half
of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to
store let alone analyze or display. This course is to help students
understand the hardware and software aspects of this problem and give them
the techniques they need to make the best use of their data.

The course is designed for biologists who need to make measurements on 3D
microscopical data sets and then display the results in an effective
manner. The course will be taught on SGI, Macintosh and IBM-compatible
computers. A wide variety of software designed for the 3D microscopy market
will be described, demonstrated and available for use.

Workshop Organizers

o Andreas Kriete Giessen University, DE
o Felix Margadant University of Sydney, Au
o Ping Chin Cheng State U. of New York, Buffalo
o Elaine Humphrey University of British Columbia
o Glen MacDonald University of Washington

PLAN OF INSTRUCTION
Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations
followed immediately by hands-on laboratory sessions using a variety of
workstations. Students will "learn-by-doing" with two to a machine. Lab
handouts will describe some specific exercises to be performed on "canned"
data sets. Facilities and supervision will be available until 11:00 PM, for
students to work on their own data.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Feb 5 19:13:42 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 05 Feb 2001 17:06:18 -0800
Subject: Re: Nikon 990, what is the best set-up , focus method and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I found that the 990 has specific applications, rather than
being of a broad nature. It is not good for DIC metallurgical
work since color balance is not effective. It is not bad for
BF. It does do a nice job on a stereo scope. with color
balanced lighting, the auto white balance will work well.
The auto exposure is also very functional. For focusing,
I used the AF mode with the zoom set to wide and use the
scope focus for all focusing. The LCD display works fine
for focusing. The critical part is to use the remote release
cable. Otherwise, operation is a real pain. I typically
had to take 3-5 shots to get one that was acceptable.
Just a minor inconvenience. I shot at high rez, JPEG.
I have not tried high rez TIFF mode.

Manual operation may be an ultimately better approach,
but I have not worked much with this mode. The 990 is
OK for quickie shots and proofs but for my work, I need
higher pixel resolution and the ability to control gamma,
and highlight/shadow regions. Also, the 990 lacks
the provision for specific manual color temperature
setting on a per-region basis. This is not an issue
with a stereo. But it sure is with a compound scope.

gary g.


At 03:05 PM 1/31/01, you wrote:

} I have played w/ my Nikon 990 a while. I am very impressed with the camera.
}
} I have a metallurgical and a stereo microscope and I connect the Nikon using
} an eyepiece adaptor.
}
} My question is: what is the best focus method and what is a suitable
} external monitor.
}
} I would assume a manual focus set at some reasonable focal length, perhaps
} the distance the eye would perceive an object when viewed in the eye piece.
} They fine focus could be done with the LM?
}
} An external monitor seems to be required. The manual indicates NTSC or PAL
} as video output. I assume that means only low resolution output, so no need
} to spend extra for a high resolution monitor? Also, the LCD monitor
} indicates 110,000 dots. Which I guess would be in the ball park of 300
} lines and 400 pixels, so it is hard to image Nikon would put much technology
} to produce extra resolution for the video output since only a small fraction
} of users would ever connect to a monitor.
}
} Has anyone out picked a monitor they are happy with?
}
} Ric
}
} SMARTech
} 860-491-3299
} www.semguy.com
} 19 Cornwall Drive
} Goshen CT 06756
}
} SMARTech
} 860-491-3299
} www.semguy.com
} 19 Cornwall Drive
} Goshen CT 06756



From daemon Mon Feb 5 20:02:24 2001



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Tue, 6 Feb 2001 10:58:19 +0900
Subject: Re: Pt/C evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use small Pt/C "chips" (cylinder shaped ... about 1.5 mm diameter and 3
mm long). The chip is held between two (not sharpened) carbon rods in the
evaporator. The "faces" of the rods are flat with a small hole in the center
(this is where the chip is held). The covering evaporated this way has very
small grain size and the process is "painless" compared to the drop method.
If you are interested I can check for more details (manufacturer of the
chips, composition, price etc.).

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------



From daemon Mon Feb 5 21:58:10 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 05 Feb 2001 19:54:18 -0800
Subject: Re: prepared slides question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might consider Ward's Scientific prepared slides:

93-8015 salivary glands; illustrating giant chromosomes
93-8016 H&E stain; chromosomes in prophase

These slides are about $12 each.

800-962-2660
http://www.wardsci.com

Rochester, NY

gary g.



At 10:08 PM 2/4/01, you wrote:

} } Email: kmccleary54-at-hotmail.com
} } Name: Kevin McCleary
} } School: South Peace Secondary School
} } State: British Columbia, Canada
} }
} } Question: I am anticipating the purchase of prepared slides for an
} } introduction to cells in my grade ten science classes.Ý Will chromosomes be
} } easily viewed in smears of Drosophila (advertized as giant chromosomes)?
} } If not, could you suggest alternate subjects.Ý Our light microscopes are of
} } standard high school quality.
} }
} _________________________________________________________________________
} Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Tue Feb 6 00:58:00 2001



From: COURYHOUSE-at-aol.com
Date: Tue, 6 Feb 2001 01:52:33 EST
Subject: wanted x ray history " on the trail of the invisible light"

Contents Retrieved from Microscopy Listserver Archives
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Notice you are in the radiology area, if you ever run across a copy of

'on the trail of the invisible light'

About the history of x-rays, lets us
know, we need a copy for the museum's reference library....

thanks Ed Sharpe archivist for SMECC
}
} } Subj: Finding Reprint Authors' E-Mail Addresses
} } Date: 2/5/01 7:29:02 PM US Mountain Standard Time
} } From: gordonr-at-Ms.UManitoba.CA (Richard Gordon)
} } To: microscopy-at-sparc5.microscopy.com
} }
} }
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Eric Cowdrey and I have put together a web page that makes it
} } relatively easy to find the e-mail addresses of academic colleagues,
} } for the purpose of requesting reprints:
} }
} } Finding Reprint Authors' E-Mail Addresses
} }
http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html
} }
} } There are no a d v e r t i s e m e n t s or costs. If you use it,
} } please let us know of omissions, corrections, or improvements you'd
} } like,


From daemon Tue Feb 6 08:00:55 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 06 Feb 2001 07:52:20 -0600
Subject: Re: Sand Request for Microscopy Education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote:
} Past sand donations from listserver subscribers helped start the MSA sand
} collection. This collection is freely given to educators to use with
} Microscopic Explorations and other educational programs.

What exactly happens in Activity 8 of Microscopic Explorations?
Are you looking for sand with special characteristics?

- John



From daemon Tue Feb 6 11:14:11 2001



From: operaciones-at-mirameonline.com
Date: Tue, 6 Feb 2001 11:10:43 -0600 (CST)
Subject: Hemos visitado su pagina y solicitamos contactarlo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



De nuestra mayor consideración:

Acostumbramos navegar la web en busca de sitios y páginas web interesantes, relacionados a la educación en todo el mundo, para establecer nuevos contactos y relaciones como objetivo principal.

De esta manera hemos encontrado su email y creemos interesante solicitarles, por favor, que visiten nuestra página relacionada al ámbito de la astronomía, biología, geología, oceanografia a través planetarios móviles, telescopios, sunspotters, etc, y servicios a las escuelas.

http://www.starlab.webprovider.com

De hacernos el favor de correspondernos, con gusto esperaremos sus opiniones, sugerencias y/o propuestas.

Además, si nos envia su dirección postal, le enviaremos gratuitamente completa información a todo color. Muchísimas gracias.

Ingeniero Alejandro Vega
Representante de STARLAB para Latino America

starlab2000-at-ciudad.com.ar

te: 54 11 4572 5800
fx: 54 11 4545 5114

Salvador Maria del Carril 2341 - Buenos Aires (1419) - Argentina




From daemon Tue Feb 6 11:14:11 2001



From: JODI SCHWARZ :      schwarzj-at-bcc.orst.edu
Date: Tue, 06 Feb 2001 09:10:20 -0800
Subject: Antibody subtraction?

Contents Retrieved from Microscopy Listserver Archives
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Hello -

I have a polyclonal antibody that was made against a protein that was
cloned from sea anemone and then expressed in bacteria. The problem is
that in TEM immunocytochemistry of sea anemone tissue, this antiserum is
labelling not only my protein of interest, but also a homologous protein
that occurs in an intracellular symbiont. I know that these are two
separate proteins because Westerns of the sea anemone show a 32kD protein
while Westerns of the symbiont show a 50kD protein. I want to tease apart
which protein localizes only to the host tissue and which one localizes to
the symbiont.

Does anyone know of any protocols to somehow do a subtraction so that I am
left with a subpopulation of antibodies that recognize only the sea anemome
protein and a separate subpopulation that recognizes only the symbiont
protein? Then I could do TEM on each partner, separately.

Thanks so much for your help!

Jodi Schwarz
______________________________________________________________

Jodi Schwarz phone: 541-737-4358
Zoology Department email: schwarzj-at-bcc.orst.edu
3029 Cordley Hall
Oregon State University
Corvallis, OR 97331
_______________________________________________________________


From daemon Tue Feb 6 12:27:27 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 6 Feb 2001 12:21:02 -0600
Subject: Re: Antibody subtraction?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you could make a homogenate of the symbiont and pre-absorb your
antisera prior to staing the anemone.




}
}
} Hello -
}
} I have a polyclonal antibody that was made against a protein that was
} cloned from sea anemone and then expressed in bacteria. The problem is
} that in TEM immunocytochemistry of sea anemone tissue, this antiserum is
} labelling not only my protein of interest, but also a homologous protein
} that occurs in an intracellular symbiont. I know that these are two
} separate proteins because Westerns of the sea anemone show a 32kD protein
} while Westerns of the symbiont show a 50kD protein. I want to tease apart
} which protein localizes only to the host tissue and which one localizes to
} the symbiont.
}
} Does anyone know of any protocols to somehow do a subtraction so that I am
} left with a subpopulation of antibodies that recognize only the sea anemome
} protein and a separate subpopulation that recognizes only the symbiont
} protein? Then I could do TEM on each partner, separately.
}
} Thanks so much for your help!
}
} Jodi Schwarz
} ______________________________________________________________
}
} Jodi Schwarz phone: 541-737-4358
} Zoology Department email: schwarzj-at-bcc.orst.edu
} 3029 Cordley Hall
} Oregon State University
} Corvallis, OR 97331
} _______________________________________________________________

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Feb 6 13:00:52 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 6 Feb 2001 13:57:05 -0500
Subject: Re: Antibody subtraction?

Contents Retrieved from Microscopy Listserver Archives
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I have a polyclonal antibody that was made against a protein that was
cloned from sea anemone and then expressed in bacteria. The problem is
that in TEM immunocytochemistry of sea anemone tissue, this antiserum is
labelling not only my protein of interest, but also a homologous protein
that occurs in an intracellular symbiont. I know that these are two
separate proteins because Westerns of the sea anemone show a 32kD protein
while Westerns of the symbiont show a 50kD protein. I want to tease apart
which protein localizes only to the host tissue and which one localizes to
the symbiont.

Does anyone know of any protocols to somehow do a subtraction so that I am
left with a subpopulation of antibodies that recognize only the sea anemome
protein and a separate subpopulation that recognizes only the symbiont
protein? Then I could do TEM on each partner, separately.

Thanks so much for your help!

Jodi Schwarz

Dear Jody,
I would try affinity chromatography. Make two affinity columns by
attaching each of the two proteins of interest to column material that is
designed for this purpose. Then run the polyclonal antibody through one or the
other. You can collect the ab that passes through one column, then detach the
ab that sticks, then do the same with the other column (this gives ab that
sticks to neither, thus should be removed, ab to the 32 kD protein which doesn't
cross-react, ab to the 50 kD protein which also doesn't cross-react, and ab
which reacts with both proteins). I don't have a detailed protocol, since I've
never done it; I'd check Methods in Enzymology as a start. Good luck.

Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Tue Feb 6 14:09:34 2001



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Tue, 6 Feb 2001 15:02:56 -0500
Subject: MAT: Position Open (SIMS)

Contents Retrieved from Microscopy Listserver Archives
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Position Open – Immediately

SIMS Research Assistant Professor or Analyst


A position is open for SIMS Research Assistant Professor (Ph.D. required) or
Analyst (BS degree or higher required) at the North Carolina State
University Analytical Instrumentation Facility (AIF).

Duties and responsibilities include: day to day operation and
maintenance of a Cameca IMS-6F SIMS instrument, stylus profilometers, and
sample preparation devices such as plasma metal coaters, vacuum ovens, etc;
assistance with scheduling of access to and oversight of the above
instrumentation and the SIMS laboratory and participation in SIMS analytical
development and related research. A BS higher degree or higher is required
(Ph.D. desired) in analytical chemistry or a materials related discipline
(non biological) along with some hands on analytical experience in a
multi-user SIMS facility and some working knowledge of SIMS. Experience in
vacuum equipment and/or electronics maintenance; experience with
microcomputers, both PC and Unix; and experience with analysis of
semiconductor or other non biological materials a plus.

Please send resume and three letters of reference to: Phil
Russell, Director; Analytical Instrumentation Facility; North Carolina State
University; Box 7531, Room 118A EGRC; 1020 Main Campus Drive; Raleigh, NC
27695-7531 or email PRUSSELL-at-NCSU.EDU.

North Carolina State University is an Equal Opportunity and Affirmative
Action Employer. ADA Accommodations: Phil Russell, prussell-at-ncsu.edu,
919-515-7501
______________________________
Roberto Garcia
NCSU/Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh NC 27695-7531
P: (919) 515-8628
F: (919) 515-6965
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm



From daemon Tue Feb 6 16:13:02 2001



From: Young, Gene (GP) :      GPYOUNG-at-dow.com
Date: Tue, 6 Feb 2001 17:07:54 -0500
Subject: RE: dusty Polaroid DMC

Contents Retrieved from Microscopy Listserver Archives
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We've seen this problem on all of our DMC and DMCie cameras (four total).
We would also be VERY interested in a permanent solution. We've had the 3
DMCie cameras since early last year. They all had to be serviced after a few
months, and now they are showing the same signs again.

Gene Young
Research Technologist
Analytical Sciences, SMX Group
The Dow Chemical Company

Michael Simko wrote:

{ {Our laboratory owns a Polaroid DMC digital camera for acquiring digital
images from a Nikon Optiphot upright optical microscope. We have the need
to determine the cleanliness of polished steel samples and capture clean,
accurate digital images. This is a very demanding application and the
presence of any dust creates unacceptable spots on the final images.

The optics in the microscope and camera mounts have been checked multiple
times and are spotless. When critical images are needed, Polaroid film is
used which works wonderfully. Obviously we would prefer to capture the
images electronically. On visual inspection, the camera chip itself
appears to have dust on the surface. We have been told that we should not
attempt to service the unit ourselves and for about five hundred dollars we
could have factory service. However, we are also told that with the
mechanical shutter action, the problem will recur in short order.
Unfortunately, we cannot tolerate the loss of productivity taken by sending
the camera in for this kind of service at frequent intervals.

Does anyone have a similar problem with this camera? Can anyone suggest a
possible solution to this dilemma? I speculate that electrostatic forces
may be keeping the dust in place. Bursts of canned air will not remove the
dust but I would be willing to try something else. I am afraid that the
camera may not be adequate for these most delicate applications and we may
need to find another unit to meet our needs. Any assistance would be
greatly appreciated. Please contact me with any suggestions and I will
respond with the results. Thank you in advance for your help.} }

Michael Simko
Research Manager ? Metallography
U. S. Steel Research and Technology Center
msimko-at-uss.com


From daemon Tue Feb 6 16:27:13 2001



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Tue, 6 Feb 2001 16:13:15 -0600 (CST)
Subject: Re: Sand Request for Microscopy Education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of {4.3.2.7.0.20010206075127.02837400-at-pc}
from John Foust {jfoust-at-threedee.com} :
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote:
} } Past sand donations from listserver subscribers helped start the MSA sand
} } collection. This collection is freely given to educators to use with
} } Microscopic Explorations and other educational programs.
}
} What exactly happens in Activity 8 of Microscopic Explorations?
} Are you looking for sand with special characteristics?
}
} - John
}
When we do it with the Minnesota Microscopy Society we have a sneaker with sand
stuck on the bottom, and have the students perform a "forensic investigation" to
see if they can determine where the sneaker has been. They are given samples of
sand from various locations and have to match them by color, shape, grain size,
shell content etc. Depending on the age range it may help to motivate them by
saying the sneaker came from a pirate who buried some treasure and we want to
find it. We typically have half a dozen samples to compare with - Florida beach,
White Sands NM, Duluth MN, California beach, Hawaii beach and Connecticut beach
amongst others. You can see examples of them at our web site
http://resolution.umn.edu/MMS/ProjectMicro/ (if you get the TV program "Popular
Mechanics for Kids" look for our desert sand images there too).

There are other ways to use the sand, but getting them to look carefully at each
one and describe it carefully is the overall aim.


Stuart





__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 Fax:(612) 625-5368



From daemon Tue Feb 6 16:27:32 2001



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Tue, 06 Feb 2001 14:23:56 -0800
Subject: [Fwd: Re: confocal laser scanning confocal microscope LSM-410 (Zeiss)]

Contents Retrieved from Microscopy Listserver Archives
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My boss asked me if I could forward this to all of the listers. If you
are interested, you call call him or myself.
Jo Dee Fish

Dear all,

We are offering our confocal laser scanning confocal microscope LSM-410
(Zeiss),
equipped with:

1. internal 543 nm and external 488 and 514 nm lasers
2. Great set of optics ( air, water, glycerol, and oil immersion lenses)
3. New Pentium computer.

If you are interested in this instrument let me know.

--
Edward Monosov, Ph.D.

Director
Cell Analysis & Histology Facility
The BURNHAM INSTITUTE
10901 N. Torrey Pines Rd, La Jolla, CA 92037
Tel: (858) 646-3100
Office (r.#5144): ext. x3206
MP Confocal Microscopy (r.#5105): ext. x3466
Fluorescent Microscopy (r.#5118): ext. x3469
Electron Microscopy (r.#5121 & ##5123): ext. x3686

FAX (858) 646-3196;
E-mail: emonosov-at-burnham-inst.org


From daemon Tue Feb 6 18:21:37 2001



From: Yanfa Yan :      yanfa_yan-at-nrel.gov
Date: Tue, 06 Feb 2001 17:12:06 -0700
Subject: Position Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Analytical Microscopy Group at the National Renewable Energy Laboratory
(NREL) in Golden Colorado, is seeking applicants for a research associate
position. The main responsibility is to carry out TEM, HREM, and EELS
characterization of epitaxial and polycrystalline semiconductor thin films
for photovoltaic applications. Current research topics include: 1. Defect
generation and reduction using lateral epitaxial overgrowth, and 2. The
microstucture, chemistry and electrical behavior of extended defects in
semiconductors.

A Ph.D. in materials science or a related field, and a strong background in
transmission electron microscopy are required. Experience with EELS and
HREM image simulation is highly desirable. Good communication skills
(verbal and written ) are essential.

Interested individuals should submit a resume, three selected publications,
and the names of three references to:

Dr. Mowafak Al-Jassim
NREL
1617 Cole Blvd.
Golden, CO 80401
Fax: (303) 384-6446
e-mail: mo-at-nrel.gov



From daemon Tue Feb 6 18:54:30 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 6 Feb 2001 16:50:07 -0800
Subject: Re: Sand Request for Microscopy Education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote:
} } Past sand donations from listserver subscribers helped start the MSA sand
} } collection. This collection is freely given to educators to use with
} } Microscopic Explorations and other educational programs.
}
} What exactly happens in Activity 8 of Microscopic Explorations?
} Are you looking for sand with special characteristics?
}
} - John -

"Microscopic Explorations" is the teacher's manual for Project MICRO, MSA's
middle school outreach program. It teaches observation more than it
teaches microscopy, and the message that sand delivers in that context is
that sand is DIFFERENT. Students look at it, describe it, and locate its
point of origin on a globe; further inquiry is encouraged. It can lead to a
geography lesson, which is why Joe has run out of sand from other
continents. Or geology, crystallography, malacology, whatever. So ANY
"special characteristics" are a real plus for a creative teacher. Please
donate, and provide whatever information you can, in brief form. I don't
think he has any industrial sands; glassmakers and others, please note!


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Feb 6 20:16:51 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 06 Feb 01 18:21:37 -0800
Subject: RE: Antibody subtraction?

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Reply to: RE: Antibody subtraction?
I have a polyclonal antibody that was made against a protein that was
} cloned from sea anemone and then expressed in bacteria. The problem is
} that in TEM immunocytochemistry of sea anemone tissue, this antiserum is
} labelling not only my protein of interest, but also a homologous protein
} that occurs in an intracellular symbiont. I know that these are two
} separate proteins because Westerns of the sea anemone show a 32kD protein
} while Westerns of the symbiont show a 50kD protein. I want to tease apart
} which protein localizes only to the host tissue and which one localizes to
} the symbiont. }
} Does anyone know of any protocols to somehow do a subtraction so that I am
} left with a subpopulation of antibodies that recognize only the sea anemome
} protein and a separate subpopulation that recognizes only the symbiont
} protein? Then I could do TEM on each partner, separately.
}

A simple thing to try is to take Western blots of the sea anemone and cut up the band where you know the symbiont protein is present (buyt excluding the sea anemone protein).
Incubate this membrane fragment with your diluted antibody. Anti-symbiont antibody binds to the blotting membrane leaving the antibodies to the sea anemone protein in suspension. The adsorbed antibody can be applied directly to your sample. It may work after one incubation, or you may need multiple exposure to the blotting membrane.
You can of course use only one membrane fragment because you can strip off bound antibody after each use.
To examine the symbiont protein, use the part of the Western with the sea anemone protein as the adsorbant.

You could also incubate the diluted antibody with homogenized bacteria (the ones in which the protein was expressed, and which brobably contaminated the initial antigen preparation). I bet that removes the extra band too.

Paul Webster



Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


JODI SCHWARZ wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello - }
} I have a polyclonal antibody that was made against a protein that was
} cloned from sea anemone and then expressed in bacteria. The problem is
} that in TEM immunocytochemistry of sea anemone tissue, this antiserum is
} labelling not only my protein of interest, but also a homologous protein
} that occurs in an intracellular symbiont. I know that these are two
} separate proteins because Westerns of the sea anemone show a 32kD protein
} while Westerns of the symbiont show a 50kD protein. I want to tease apart
} which protein localizes only to the host tissue and which one localizes to
} the symbiont. }
} Does anyone know of any protocols to somehow do a subtraction so that I am
} left with a subpopulation of antibodies that recognize only the sea anemome
} protein and a separate subpopulation that recognizes only the symbiont
} protein? Then I could do TEM on each partner, separately.
}
} Thanks so much for your help! }
} Jodi Schwarz
} ______________________________________________________________
}
} Jodi Schwarz phone: 541-737-4358
} Zoology Department email: schwarzj-at-bcc.orst.edu
} 3029 Cordley Hall
} Oregon State University
} Corvallis, OR 97331
} _______________________________________________________________
}
}
}
} RFC822 header
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}
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 7 02:07:44 2001



From: Paul Webster :      pwebster-at-hei.org
Date: 2/6/01 10:57 AM
Subject: FWD: Re: Antibody subtraction?

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--------------------------------------

Dear Jody,
I would try affinity chromatography. Make two affinity columns by
attaching each of the two proteins of interest to column material that is
designed for this purpose. Then run the polyclonal antibody through one or the
other. You can collect the ab that passes through one column, then detach the
ab that sticks, then do the same with the other column (this gives ab that
sticks to neither, thus should be removed, ab to the 32 kD protein which doesn't
cross-react, ab to the 50 kD protein which also doesn't cross-react, and ab
which reacts with both proteins). I don't have a detailed protocol, since I've
never done it; I'd check Methods in Enzymology as a start. Good luck.

Yours,

Bill Tivol

Reply:
Hi Bill,

Although an accepted method for antibody purification, there are a couple of problems with affinity purification. One is that there has to be enough purified antigen (protein) available to put onto a column. It is increasingly clear that many antigens are just not that abundant and so other methods have to be found to relace this form of purification. Small strips of nitrocellulose into which has been embedded a small amount of the protein (Western blotting), purified by gel electrophoresis, are very useful for small volumes. These strips are useful both for affinity purification of small amounts of antigen, and for subtractive adsorption. This is where antigens are added to antibodies to remove specific binding species. This leaves the specific antibodies of interest in suspension.

The other main problem with affinity chromatography is more theoretical than real. Antibody binding to antigens is variable. Some antibodies will have high affinity and bind so tightly that it is almost impossible to remove them from the antigen. If all antibodies to be used for immunocytochemistry go through an affinity purification step it is logical to assume that many of the very best antibodies will have been removed before we even start to label with them.

As I said, this is more a theoretical problem I put forward than one I have experienced. I have not researched studies that may have observed this effect in practice, but I am sure it could possibly occur. Has anyone seen or heard about this effect occurring in practice?

Regards,

Paul Webster.


Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 7 04:46:40 2001



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Wed, 07 Feb 2001 02:41:11 -0800
Subject: SEM TV rate frame integration

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I am seeking a device which will provide live NTSC frame integration or
frame averaging for my full TV rate SEM. I would prefer a "stand alone
box", but a PC card with a live-pass through would be OK.

I already own an Optical Electronics 67156 Frame integrator which will
integrate 7 frames. I would like the capability to select more
integration.

I have made a half-hearted effort to contact vendors on this matter, but
I am not confident that I uncovered the full range of solutions.

Thank you.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233.




From daemon Wed Feb 7 07:12:15 2001



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Wed, 7 Feb 2001 07:07:13 -0600
Subject: Re: Sand Request for Microscopy Education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In Activity 8 students examine sand and compare color, size, and shape of
sand grains. They also look for crushed rocks, shells, bones, minerals,
etc. Then they locate the sand on a map, thus linking science and geography.

Joe Neilly
Abbott Laboratories




jfoust-at-threedee.com on 02/06/2001 09:58:29 AM
To: microscopy-at-sparc5.microscopy.com, joe.p.neilly-at-abbott.com
cc:


At 08:25 AM 2/5/01 -0600, Joseph Neilly wrote:
} Past sand donations from listserver subscribers helped start the MSA sand
} collection. This collection is freely given to educators to use with
} Microscopic Explorations and other educational programs.

What exactly happens in Activity 8 of Microscopic Explorations?
Are you looking for sand with special characteristics?

- John







From daemon Wed Feb 7 12:25:56 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 07 Feb 2001 13:19:29 -0500
Subject: Re: Pt wire diameter + Pt/C evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would prefer not to take sides in the Darley/Garber discussion
concerning Pt/C evaporation, but our research over the years clearly
shows that it is the simultaneous nature of Pt/C evaporation which
results in the finer grain structure.
To achieve this we have long supplied Pt/C pellets (50/50) which as Dr.
Danev described are placed between two flat faced carbon rods. If
further information is required feel free to contact us or visit our web
page.

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



Jim at ProSciTech wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Chuck's reply without the customary inclusion of the previous contribution is
} difficult to understand. I have now added my previous contribution and the
} original request below.
} The hanging drop method results largely in Pt evaporation followed by carbon.
} The method also requires breaking the vacuum and is therefore more trouble. As
} Sergey Ryazantsev in his thoughtful contribution explained (I erased that
} because there was no need to reply), its the simultaneous nature of Pt/C which
} yields the finer grain structure. Sergey also explained that the other means of
} achieving such fine grain is electron beam evaporation, but that requires
} special equipment.
} I explained in my previous contribution why the thinner wire is required.
} 30 years ago I used many meters annually of the 0.1mm Pt for freeze etching and
} Kleinschmitt/ DNA rotary shadowing.
} Nothing for it Chuck: you will need to stock 0.1mm Pt wire like all other major
} suppliers.
} Don't be overly concerned, very few people use these techniques now; in fact
} you could purchase your minor requirements from us!
} Disclaimer: its too obvious, we sell small quantities of 0.1mm Pt.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Sunday, February 04, 2001 12:43 AM, Garber, Charles A.
} [SMTP:cgarber-at-2spi.com] wrote:
} } ------------------------------------------------------------------------} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.} }
} }
} } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} }
} } The "main" way Pt/C I thought was being evaporated today is the "bead on
} } carbon" method, that is, where the wire is coiled up, around a pin of
} } diameter slightly larger than the diameter of the neck on the carbon rod,
} } and then, with some help from some good Style #5 tweezers, it is pulled onto
} } the sharpened neck of the carbon rod. The bell jar is lowered over the
} } carbon rods and holders, and wearing the appropriate eye protection, the
} } power is slowly turned up, slowly heating the carbon rods and wound Pt wire.
} } At some point the miracle happens: The Pt melts, and surface tension
} } effects cause it to form (in an instant) a nice little "bead" (it looks like
} } the textbook "sessile drop") firmly attached to the carbon rod once the
} } heating is stopped. After cooling down, the rod with the drop is rotated
} } around so that it is facing where the samples will be, the bell jar is then
} } loaded, pumped down and evaporation of Pt/C can be done simultaneously this
} } way.
} }
} } Indeed I don't think it is possible to evaporate Pt wire **without** the
} } formation of the sessile drop, therefore, how the wire is originally spread
} } out (on the rod neck) does not matter!
} }
} } A note not related to the original question: The sharpened "neck" should be
} } sharpened to a diameter not more than 3/16" (4.75 mm). The method will not
} } work as well if the neck diameter is larger than this. If you are
} } sharpening your own rods, make sure you are using rods of higher rather than
} } lower density, otherwise the rod will not have the mechanical properties
} } needed to sharpen down to this diameter.
} }
} } I had a phone call on Friday from a customer asking about 0.1 mm (e.g. 4 mil
} } ) wire since a previous posting seemed to attribute to it something special
} } (relative to 0.2 mm/8 mil) wire. I told the caller that we did not believe
} } that to be so, that for the practice of the sessile drop method, either
} } diameter wire would "bead up" just as quickly and easily, and the only
} } difference between the two diameters would be one of cost, since the cost to
} } draw the same mass in 4 ml is obviously going to be higher than that to draw
} } 8 mil. Actually some years ago we came into some 10 mil Pt wire, and we
} } found it could be used just as easily to make the sessile drop.
} }
} } Furthermore since either diameter wire could be used to create a drop of
} } equal size, the actual evaporation time would be independent of the diameter
} } of the starting wire.
} }
} } In the end, we came upon the novel conclusion that I would make a posting
} } and subject my advice to the most stringent (microscopy) peer review panel
} } in the world, namely the listserver. Am I not correct, in that the end
} } result will be the same irrespective of whether 4 mil or 8 mil wire is used?
} }
} } Chuck
} }
} } Disclaimer: SPI Supplies is a long time supplier of the high purity Pt wire
} } that is used for evaporation in electron microscopy applications.
} }
} } ===================================================
} } Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} } President 1-(800)-2424-SPI
} } SPI SUPPLIES FAX: 1-(610)-436-5755
} } PO BOX 656 e-mail: cgarber-at-2spi.com
} } West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
} }
} }
} } Look for us!
} } ############################
} } WWW: http://www.2spi.com
} } ############################
} } ==================================================
}
} } From: Jim at ProSciTech [SMTP:jim-at-proscitech.com]
} Sent: Friday, February 02, 2001 9:59 AM
} To: 'Heinrich Matthies'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: rotary shadowing, platinum coating
}
} } I translate (x25.4) the funny measure 0.008 " to 0.2mm.
} For Pt/C coating the more commonly used thickness is 0.1mm wire. If you work
} out the cross section area of both wires you would find that you are using
} considerably more Pt than the 50 to 75mm of 0.1mm wire otherwise used.
} I suggest that you switch to the thinner wire because this would cover more of
} the 1.4mm carbon cylinder. Therefore this would take longer (in time) to
} evaporate. This is important because you need more than 5 seconds of
} evaporation to obtain an even coating - with the specimen rotating at perhaps
} 30 RPM.
} What do you mean: " } at lower amps, less than 24, I don't see any Pt on the
} test paper"?
} The C would be much more visible than the Pt and normally you would not see the
} Pt on filter paper.
} The test is in the looking.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Thursday, February 01, 2001 9:00 AM, Heinrich Matthies
} [SMTP:hjmatthies-at-ucdavis.edu] wrote:
} }
} }
} } Hello,
} }
} } I'm plan to attempt to rotary shadow a motor protein using platinum on a
} } carbon rod and then coat with carbon. At the moment, I am trying to
} } evaporate the platinum from the carbon electrode and am having difficulties.
} }
} } We have a denton vacuum LLC with a bench top turbo III high vacuum
} } evaporator. We wind one inch of platinum (Pt) wire on a nail (0.045) and
} } then we load this 1 inch of 0.008 Pt wire onto a 0.04 tip of a 1.5mm carbon
} } rod. The wire is pushed toward the solid carbon rod coming from the other
} } side and all of the loops are tight (touch each other). The "spring" of Pt
} } wire is tightly wound around the carbon wire. We pull a vacuum to about 5
} } x 10-5 torr and bring the amperage to 10 amps (filament 2) wait and then
} } increase the amperage. Then I've tried 20,22.24, 26 and 30 amps but
} } usually somewhere between 26-30, the carbon appears to evaporate. At lower
} } amps, less than 24, I don't see any Pt on the test paper. So at the higher
} } amps, both carbon and pt evaporate and this means we are getting too much
} } carbon rather than an initial Pt coating. Under these conditions, the Pt
} } wire only covers a short distance of the narrow tip of the carbon
} } electrode. Does anyone have any advice or experienced this problem?
} }
} } Thank you,
} }
} } Heiner Matthies
} } UC Davis
} } MCB
} } 530-754-9051


From daemon Wed Feb 7 13:45:14 2001



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Wed, 07 Feb 2001 13:51:25 -0600
Subject: FRET/FLIM Symposium Annnouncement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{bigger} The University of Texas Health Science Center will host a
symposium sponsored by

Hamamatsu Photonics KK

on

{/bigger}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} {bigger} FRET
and FLIM:

{/bigger} {/bigger} {/bigger} {/color} {/bold} {bold} {bigger} Advanced
Fluorescence Techniques for Biological Imaging

{/bigger} {/bold}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} June 8-10,
2001 {/bigger} {/bigger} {/color} {/bold} =20


{bold} at {/bold} =20


{bold} {bigger} The Sheraton Gunter Hotel

205 E. Houston St.

San Antonio, TX

{/bigger} {/bold}

{bold} {color} {param} 0000,0000,ffff {/param} {bigger} Registration Fees

Student: $175 ($200 after May 1st)

Academic/Corporate: $225 ($250 after May 1st)

{/bigger} {/color} {/bold}


Meeting, lodging and travel information may be found at:


{bold} {color} {param} ffff,0000,ffff {/param} {bigger} {bigger} http://usa.hamamat=
su.com/fretflim

{/bigger} {/bigger} {/color} {/bold}

or contact


Victoria Centonze Frohlich (mailto:frohlich-at-uthscsa.edu)


{bold} {bigger} Speakers:

{/bigger} {/bold}

Philippe Bastiaens, European Molecular Biology Laboratory (Germany)=20

Christoph Biskup Friedrich, Schiller University (Germany)=20

Robert Clegg, University of Illinois Urbana-Champaign (USA)=20

Michael Edidin, Johns Hopkins University (USA)=20

Hans Gerritsen, Utrecht University (Netherlands)=20

Jesus Gonzalez, Aurora Biosciences (USA)=20

Enrico Gratton, University of Illinois Urbana-Champaign (USA)=20

Brian Herman, University of Texas Health Science Center San Antonio (USA)=20

Thomas Jovin, Max-Planck Institute for Biophysical Chemistry (Germany)=20

Steven Kay, The Scripps Research Institute/Novartis Inc.(USA)=20

Karsten K=F6nig, Friedrich Schiller University (Germany)=20

Wen-Hong Li, University of Texas Southwestern Medical Center (USA)=20

Atsushi Miyawaki, The Institute of Physical and Chemical Research (Japan)=20

Ammasi Periasamy, University of Virginia (USA)=20

Alexander Sorkin, University of Colorado Health Science Center (USA)=20

Roger Tsien, University of California, San Diego (USA)=20





From daemon Wed Feb 7 13:48:41 2001



From: Jane LaGoy :      jlagoy-at-bodycote-imt.com
Date: Wed, 7 Feb 2001 14:49:08 -0500
Subject: THANK YOU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to everyone who sent me copies of the Gary Larson EM/LM cartoons -- I
had forgotten that the mammoth one was another one I had lost!



From daemon Wed Feb 7 16:33:02 2001



From: JODI SCHWARZ :      schwarzj-at-bcc.orst.edu
Date: Wed, 07 Feb 2001 14:41:15 -0800
Subject: thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The message below, from John Francis of Polaroid Corporation, refers to a
"Shutter Opening Utility" for cleaning dust off of the anti-aliasing filter
of the Polaroid DMC digital camera. I tried it on one DMCie camera and the
method was about 98% successful for removing dust particles between the
shutter and filter. It took 1-2 hours to reach a point where I felt like it
was as clean as I could get it. If the other 3 cameras can be cleaned this
way, it will save us considerable time and money.

Gene Young


-----Original Message-----
} From: Francis, John W [mailto:FRANCIJ-at-polaroid.com]
Sent: Wednesday, February 07, 2001 8:01 AM
To: 'Young, Gene (GP)'


Thanks to all who offered suggestions about doing an "antibody
substraction." I shall proceed posthaste!

Jodi
______________________________________________________________

Jodi Schwarz phone: 541-737-4358
Zoology Department email: schwarzj-at-bcc.orst.edu
3029 Cordley Hall
Oregon State University
Corvallis, OR 97331
_______________________________________________________________


From daemon Wed Feb 7 16:52:06 2001



From: Larry Nittler :      nittler-at-lepvax.gsfc.nasa.gov
Date: Wed, 07 Feb 2001 17:49:03 -0500
Subject: Ultramicrotome recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I am looking to buy an ultramicrotome. Any recommendations on specific
manufacturers/models?

Thanks,
Larry
--
----------------------------------------------------------------------
Larry R. Nittler Laboratory for Extraterrestrial
Physics, Code 691
Interstellar Dust Buster NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
phone (DTM): 202-478-8460
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/
----------------------------------------------------------------------


From daemon Wed Feb 7 17:51:52 2001



From: Kevin McCleary :      kmccleary54-at-hotmail.com
Date: Mon, 05 Feb 2001 00:08:37 -0600
Subject: prepared slides question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



} Email: kmccleary54-at-hotmail.com
} Name: Kevin McCleary
} School: South Peace Secondary School
} State: British Columbia, Canada
}
} Question: I am anticipating the purchase of prepared slides for an
} introduction to cells in my grade ten science classes.Ý Will chromosomes be
} easily viewed in smears of Drosophila (advertized as giant chromosomes)?
} If not, could you suggest alternate subjects.Ý Our light microscopes are of
} standard high school quality.
}
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.








From daemon Wed Feb 7 17:52:55 2001



From: Damian :      dneuberger-at-mindspring.com
Date: Mon, 05 Feb 2001 00:07:32 -0600
Subject: Midwest Microscopy&Microanalysis Mtg Feb. 13, 2001

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



MIDWEST MICROSCOPY AND MICROANALYSIS SOCIETY, INC. (MMMS)
AFFILIATE OF THE MICROSCOPY SOCIETY OF AMERICA

MEETING ANNOUNCEMENT

JOINT MEETING WITH THE SOCIETY FOR APPLIED SPECTROSCOPY
Tuesday, Feb. 13, 2001

At Unilever HPC USA, located at 3100 Golf Road, Rolling Meadows, IL. See
directions for more details.

Social Hour: 5:30 pm
Dinner: 6:00 pm
Speaker: 7:00 pm
_________________________________________________________________
The Infrared Microprobe in Production and Research
By Koichi Nishikida

Abstract
Unlike the visible microscope and electron microscope, which respectively
use visible light and electrons to magnify the sample, the infrared
microscope does not utilize infrared wavelengths to magnify the sample
image. Instead, a visible microscope is modified, so that a magnified
sample image is observed and, at the same time, an infrared beam is passed
through the microscope to obtain an infrared spectrum of the sample.
Therefore, the "IR microscope" should be better named the "IR microprobe".

Applications of the IR-microprobe cover applications from forensic
} analysis
to defect analysis, reverse engineering in the production industry, and
medical diagnostic research.

In this talk, I will show how the IR-microprobe has contributed to quality
improvements in the production industry, as well as recent research in
medical applications. Briefly, I will address the evolution of this
technique over the past five decades.
*********************************************************************
Please make your dinner reservations for the upcoming meeting by calling
(847)734-3712. Leave your name, company affiliation and the number of
reservations. Please call by noon on February 9th, so that proper
arrangements can be made.
Dinner Cost:
M3S Members: $25
M3S Students: $10
Nonmembers: $30


Directions to Unilever, Rolling Meadows, Illinois

From the Chicago Area:
Kennedy Expressway to I-90 West (Rockford);
I-90 West to Arlington Heights Rd.;
North to Golf Rd. (2nd traffic light);
Turn left (West) onto Golf Rd. for approximately 2 miles.
The Unilever R&D facility is on the right, just before Hwy. 53, across from
the forest preserve. Follow the entrance road all the way to the last
parking lot (North lot) and enter the building through the far glass doors.


From I-290 Eisenhower Expressway:
Stay on I-290 towards Rockford;
Exit on Golf/Higgins Rds.;
Continue North to Golf Rd.;
Turn east on Golf Rd.
Unilever is1/4 mile left on Golf Rd., across from the forest preserve.
Follow the entrance road all the way to the last parking lot (North lot)
and enter the building through the far glass doors.


From the Northern Suburbs (Route 53 South):
Exit Woodfield Drive/Golf Rd. (58);
Continue to first light;
Turn left at first light;
Continue to Golf Rd (58);
Turn right (East) on Golf Rd.;
Unilever is1/4 mile left on Golf Rd., across from the forest preserve.
Follow the entrance road all the way to the last parking lot (North lot)
and enter the building through the far glass doors.

If you have any questions about the meeting, please direct your inquiries
to the phone number below. For questions about MMMS, please contact:

Dr. Damian Neuberger
Senior Research Scientist
Baxter Healthcare, Corp.
(847) 270-5888
damian_neuberger-at-baxter.com










From daemon Wed Feb 7 20:44:40 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 07 Feb 2001 18:25:45 -0800
Subject: Re: dusty Polaroid DMC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suppose that this is nice, but one must wonder why such
kludge operations are necessary in the first place? It seems
to me that it is simply indicative of a basic flaw or limitation
in the unit's design. The DMC is rather old, in technology
life time terms. It was rather good in its day, but that
has passed.

If this operation does keep your investment working, that
is good. If the periodic procedure does not become too
burdensome, then the unit should serve one's needs.

I had one of these units for about 4 months and found that
it not only did not perform well but was quite unreliable.
Polaroid was very accommodating in offering a refund
in lieu of a replacement. Their scanners are quite the
opposite of their cameras, in my experience.

gg


At 02:27 PM 2/7/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Feb 8 05:30:17 2001



From: Sam Li :      samli-at-mrc-lmb.cam.ac.uk
Date: Thu, 8 Feb 2001 11:23:51 +0000 (GMT)
Subject: CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, has anyone done any study or comparison of the slow scan CCD
camera from Gatan and Tietz, for example, Model 795 from Gatan vs. Model
TemCam-F224 from Tietz? Any comments will be appreciated.

Sam Li
*******************************
Medical Research Council
Laboratory of Molecular Biology
Hills Road
Cambridge, CB2 2QH
United Kingdom
*******************************



From daemon Thu Feb 8 06:07:06 2001



From: a.asterix-at-firemail.de
Date: Thu, 08 Feb 2001 05:58:43 -0600
Subject: Dow Jones Investing Video..Commodities 6842

Contents Retrieved from Microscopy Listserver Archives
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{!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"}
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From daemon Thu Feb 8 06:55:18 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 04 Feb 2001 11:11:29 -0700
Subject: RE: SEM and LM image capture on PC

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Brian,

there are definitely many cards out there to acquire standard TV signals
from any source that adheres to that standard. These standards were
developed decades ago for TV cameras, and my personal opinion is, that they
are good for moving images (as on TV), but they lack resolution and
definition for still images (LM and SEM). However, since they cards are
usually not very expensive, and some of them do offer integration
capabilities (normally frame integration), it might be worth a shot. Also
the microscopes themselves might be offering integration capabilities. You
are limited to a resolution of 640x480 (NTSC) or 758x576 (PAL).

For better resolution you might want to look at other options as well. For
SEMs there are interfaces available (such as our ADDA II, but there are
other manufacturers also), normally available as "passive" or "active"
systems. For LM, of course, there are digital cameras with better resolution
and bit-depth (Video only carries about 8 bits of information, if you're
lucky).

Look for systems that are open for upward expansion to keep your options
open. Contact me offline if you are interested in getting more information
about the way we do that.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

At 07:03 AM 1/29/01, you wrote:
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From daemon Thu Feb 8 08:40:14 2001



From: Phoebe J Doss :      pjdoss-at-cvm.okstate.edu
Date: Thu, 8 Feb 2001 08:36:02 -0600
Subject: Sorvall MT 6000 ultramicrotome

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Hello:

I have a Sorvall MT 6000 that sections much slower than the
millimeter/second speed I have it set at. I can get it to operate
correctly by speeding up the set speed and letting it run at that setting
for awhile and then slowly taking it down to my desired sectioning speed.
I have cleaned it, but don't know what else to do. Any suggestiongs?

Thanks.

Phoebe

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Department of Physiological Sciences
264 McElroy Hall
Oklahoma State University
Stillwater, OK 74078
405-744-6765



From daemon Thu Feb 8 08:53:47 2001



From: simon watkins :      swatkins+-at-pitt.edu
Date: Thu, 08 Feb 2001 09:49:19 -0500
Subject: Up for grabs

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We have an MT5000 which works though is surplus to our needs, I am happy to
ship it to the most needy home
Simon


---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------



From daemon Thu Feb 8 08:58:37 2001



From: Wulp, Kees van der :      wulp-at-pml.tno.nl
Date: Thu, 8 Feb 2001 15:55:17 +0100
Subject: Looking for B/W CCD camera for SGI-O2

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Dear listmembers,

I am looking for B/W CCD cameras that, mounted on a microscope, can be
connected and directly controlled (not driven from an extensive package,
only by a simple driver) by a Silicon Graphics O2 workstation. The chip
should at least have 1030x1030 (about 6 micron square) pixels. It should
have 8 to 12 bit greyvalues. It will NOT be used for live images (25 full
frames in Europe), so (transmission) speed is not an issue. It should have
exposure times between 10 ms and a few seconds. The camera has to be
controlled (setting exposure time and taking an image) by the SGI-O2.
If anyone has information I hope you will be so kind as to share it with me.
Please respond directly to me (email address below).
Thank you all in advance.

Kees van der Wulp

C.J.M. van der Wulp
TNO-Prins Maurits Laboratorium
Division 3, dept. MB
Lange Kleiweg 137
2288 GJ Rijswijk
The Netherlands

Tel.: +31 15 284 3101
Fax: +31 15 284 3939 or
Fax: +31 15 284 3959
email: wulp-at-pml.tno.nl



From daemon Thu Feb 8 12:59:48 2001



From: OCONNELL-at-ltu.edu
Date: Thu, 08 Feb 2001 13:52:36 -0500 (EST)
Subject: Job Posting

Contents Retrieved from Microscopy Listserver Archives
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Am looking fo a sem operator/metallurgist/materials person
for a small optical and electron microscopy lab in the
Detroit area. If interested call 734-668-3309 or e-mail
Dick O'Connell at oconnell-at-LTU.edu

Thanks
Dick O'Connell


From daemon Thu Feb 8 14:51:03 2001



From: Sally Shrom :      sally.shrom-at-villanova.edu
Date: Thu, 08 Feb 2001 15:45:40 -0500
Subject: Ultramicrotome

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Leica Ultra-cut UCT is my favorite.
Sally Shrom



From daemon Thu Feb 8 15:36:18 2001



From: Jensen, Karen :      Karen_Jensen-at-URMC.Rochester.edu
Date: Thu, 8 Feb 2001 16:32:20 -0500
Subject: TEM IMAGE OF AMPLICONS

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Does anyone know what Amplicons (from HSV I)
look like either in cultured cells or by negative staining. I have done
several literature searches which discuss the molecular structure with
diagrams, but none of them have a TEM image.


Karen L. Jensen, M.S.
Associate Scientist & Project Manager
Electron Microscope Research Core
Pathology Department, Box 626
University of Rochester Medical Center
Rochester, NY 14642




From daemon Thu Feb 8 16:13:26 2001



From: simon watkins :      swatkins+-at-pitt.edu
Date: Thu, 08 Feb 2001 17:07:34 -0500
Subject: Up for grabs: and the winner is

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I feel like an E-Bay auction (though this has no $$s attached)... Thanks for
the tremendous interest (we received almost 50 requests). The MT5000 is on
its way to Oshkosh Wisconsin, to be placed in the lab of a starting faculty
member who is putting a new EM facility into operation.
Simon


---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------



From daemon Thu Feb 8 16:54:44 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 08 Feb 2001 17:50:41 -0500
Subject: Ion miller available

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We have a Gatan Dual Ion Mill model 600, which we no longer need. It is
complete and working (as far as we know) except that it lacks a backing
pump.

Will anyone who is interested in acquiring it please contact me (off
line, of course).
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Fri Feb 9 10:49:26 2001



From: E.M.M. Manders :      e.manders-at-chem.uva.nl
Date: Fri, 09 Feb 2001 17:26:00 +0100
Subject: Registration FOCUS ON MICROSCOPY 2001, Amsterdam, April 1-4

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***************************************************
FOCUS ON MICROSCOPY 2001, Amsterdam, April 1-4
***************************************************


We are happy to announce that about 100 people have submitted an abstract.
Although the deadline for abstract submission was a week ago (February 1),
we still receive new abstracts every day. Next week we will finalize the
program and put the final program on the web on February 19. So, if you
would like to submit you contribution for FOM 2001, please hurry!!!


On our web-pages you will find the titles of some of the presentations of
the invited speakers. With the 100 contribution from other researchers from
various fields (e.g. instrumentation, non-linear optics, 2-photon
microscopy, imaging of living cells), the conference will become a great
success.

We expect that about 250 participants will visit the conference. Since the
maximum number of participants is 300, it is important to register early !!!



***************************************************
FOCUS ON MICROSCOPY 2001, Register now !!!!
***************************************************

FOM 2001 , Amsterdam The Netherlands, April 1-4, 2001


FOM 2001 is a world leading international conference on advanced
microscopy. It is the joint meeting of the 13th International Conference on
Confocal Microscopy and the 13th International Conference on 3D Image
Processing in Microscopy.

FOM 2001 will be held at the Academic Medical Centre (AMC) of the
University of Amsterdam, Amsterdam, The Netherlands.

For more information visit: http://www.focusonmicroscopy.org


Core conference subjects:
"INSTRUMENTATION" and "IMAGE PROCESSING AND ANALYSIS"

SPEAKERS: S. Hell (Gottingen, Germany), T. Wilson (Oxford, UK), S. Kawata
(Osaka, Japan) C.J.R. Sheppard (Sydney, Astralia), C. Coggswell (Sydney,
Astralia) E. Stelzer (Heidelberg, Germany) C. Cremer (Heidelberg, Germany),
P.A. Benedetti (Pisa, Italy), A. Kriete (Giessen, Germany), H. van der
Voort (Hilversum, The Netherlands), P.C. Cheng (Buffalo, USA), G.J.
Brakenhoff (Amsterdam, The Netherlands)


Special conference subjects this year:
MULTI-PHOTON MICROSCOPY" and "MICROSCOPY OF LIVING CELLS AND TISSUE".

SPEAKERS: A. Zumbusch (Muenchen, Germany), L. Moreaux (Paris, France) A.
Diaspro (Genova, Italy), M. Muller (Amsterdam, The Netherlands), K.
Sullivan (La Jolla, USA, to be confirmed), R. van Driel (Amsterdam, The
Netherlands), R. Eils (Heidelberg, Germany), T. Mistelli (Bethesda, USA; to
be confirmed), D. Gadella (Wageningen, The Netherlands), J. Dobrucki
(Krakov, Poland), A. Houtsmuller (Rotterdam, The Netherlands), E. Manders
(Amsterdam, The Netherlands)


Conference Office:
------------------
Mrs M.P.A. Beunk-Timmers
Nicolaes Tulp Institute
PO-Box 23213
1100 DS Amsterdam
The Netherlands
Fax: +31-(0)20-6963228
Phone +31-(0)20-5668585
Web: http://www.FocusOnMicroscopy.org
E-mail: info-at-FocusOnMicroscopy.org


Local Organising Committee:
---------------------------
Prof. G.J. Brakenhoff and Dr. E.M.M. Manders


---------------
Erik M.M. Manders, PhD
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam

Visit: Kruislaan 316, Building III, room 2.07, Amsterdam, The Netherlands
Plantage Muidergracht 12, 4th floor, Amsterdam, The Netherlands
Mail: Kruislaan 316
1098 SM AMSTERDAM
The Netherlands
E-mail: e.manders-at-chem.uva.nl
Tel: +31-(0)20-5256225 (5257702)(5255136)
Fax: +31-(0)20-5256271
Web: http://wwwmc.bio.uva.nl/
http://www.FocusOnMicroscopy.org/
---------------



From daemon Fri Feb 9 11:04:45 2001



From: Valerie Woodward :      WOODWARD-at-brk.bfg.com
Date: Fri, 09 Feb 2001 11:50:19 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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please unsubscribe

Valerie P. Woodward
Senior R&D Chemist
BFGoodrich Performance Materials
Measurement Science, D/2197
Microscopy and X-ray Analysis
9921 Brecksville Rd.
Brecksville OH 44141-3289
(216) 447-5408 (voice)
(216) 447-5575 (FAX)
woodward-at-brk.bfg.com (e-mail)



From daemon Fri Feb 9 14:49:27 2001



From: George Lawton :      George.Lawton-at-UTSouthwestern.edu
Date: Fri, 09 Feb 2001 14:41:11 -0600
Subject: Need EDS help

Contents Retrieved from Microscopy Listserver Archives
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To the Listserver
I have an investigator who needs some EDS work done. My lab does not have a capability to do this type of work.

The investigator is looking for evidence of Aluminum Oxide deposition in six tissue samples of lung, brain, and skin obtained from mice exposed to and not exposed to aerosolized Aluminum Oxide.
They are willing to send the samples to any lab in the United States who can do the work for them.
Please email me if you can help.

Thanks in advance,

George

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
Fax 214-648-6408
eMail: George.Lawton-at-UTSouthwestern.edu


From daemon Fri Feb 9 17:33:31 2001



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 9 Feb 2001 17:12:01 -0600
Subject: SEM Grain Mounts

Contents Retrieved from Microscopy Listserver Archives
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List,

I am looking for a source of SEM grain mounts. Basically a 1" dia. disk of
aluminum with a patterned array of holes. Exploring buying prior to making.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Sat Feb 10 06:21:05 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Sat, 10 Feb 2001 13:13:31 +0100
Subject: IEM: First report on LR-White IEM

Contents Retrieved from Microscopy Listserver Archives
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Dear members of the listserver,

GR Newman introduced LR-White for Immuno-EM 1982/1983. The first article published was in Histochem J (1983,15,543-555). OK so far.
But there was a earlier report in the Journal of Microscopy in abstract form.
I know issue and pages from another cite (J Microscopy, 1982, p. 127, RP5-6), but I don´t know the TITLE ....!
Who can help me?
Tanks in advance,
With best regards,

Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________________
Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de
Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de



From daemon Sat Feb 10 09:50:33 2001



From: Volfova Petra :      volfova-at-chelin.chtf.stuba.sk
Date: Mon, 05 Feb 2001 00:00:46 -0600
Subject: TEM-Preparation of dispersion samples

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I would like to request of preparation samples from
poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size
for measurements by JEOL type of transmission electron microscope.
We have got some problems with dilution of latexes and with time of
staining of carbon, too. We would like to confirm a core/shell
particles morphology of samples prepared by two step seeded emulsion
polymerization. We have got some problems with magnification and contrast
expansion of core/shell particles,too.
Thank you very much for your help.

Petra Volfova, PhD. student of Department of plastics and rubber,
Slovak Technical University,Bratislava,Slovak Republic.







From daemon Sat Feb 10 12:21:28 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Sat, 10 Feb 2001 18:14:42 -0000
Subject: IEM: First report on LR-White IEM

Contents Retrieved from Microscopy Listserver Archives
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NEWMAN GR, JASANI B, WILLIAMS ED
THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITY
J MICROSC-OXFORD 127: (SEP) RP5-RP6 1982

Chris

----- Original Message -----
} From: "Michael Reiner" {Elektronenmikroskopie-at-web.de}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, February 10, 2001 12:13 PM


Dear members of the listserver,

GR Newman introduced LR-White for Immuno-EM 1982/1983. The first
article published was in Histochem J (1983,15,543-555). OK so far.
But there was a earlier report in the Journal of Microscopy in
abstract form.
I know issue and pages from another cite (J Microscopy, 1982, p. 127,
RP5-6), but I don´t know the TITLE ....!
Who can help me?
Tanks in advance,
With best regards,

Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
______________________________________________________________________
_________
Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE:
http://web.de
Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP:
http://freemail.web.de





From daemon Sat Feb 10 13:17:42 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 10 Feb 2001 11:09:42 -0800
Subject: Re: SEM TV rate frame integration

Contents Retrieved from Microscopy Listserver Archives
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I did some experiments with my SEM and external
frame grabber. The results were rather interesting.

SEM is an Amray 1910FE, which includes internal
TV signal processing, which is frame averaging and
Kalman filtering.

External frame grabber is a Soft Imaging GrabBit PCI
board controlled by their analySIS software. The
analySIS and GrabBit package will do a huge number
of frame averages. It will not do Kalman.

If I do frame averaging in the SEM at a count of 4 or 5,
I get rather good results. If I do Kalman, I get very good
results. Either of these frame processes can be frozen
in the SEM's internal buffer. That composite NTSC RS-190
video is grabbed by the GrabBit. The captured results
are those seen on the SEM's monitor.

If I do frame averaging with the GrabBit and analySIS
anywhere from 8-30 frames, the results are not
near as good as using the SEM's electronics. At
low to medium magnification ( { 10KX), running at
80 averaged frames makes no dramatic difference.
As magnification increases, the image tends to slightly
shift. This effectively kills the ability to average. However,
the SEM's internal Kalman filter will capture a very nice
image in about 2-3 seconds. And this is quick enough
to avoid image movement.

So, to answer your question, I would suggest looking for
a TV frame grabbing system which does Kalman. No
idea if such a thing exists. It would probably be an
integrated h/w + s/w product since they would have to
be closely tied. Since it is a real time process, the
software would have to process image frames directly
from the frame grabber board/interface.

If you need any other info, please contact me.

gary g.


At 02:41 AM 2/7/01, you wrote:
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From daemon Sat Feb 10 21:38:55 2001



From: neetal.timble-at-cable.alcatel.com ()
Date: Sat, 10 Feb 2001 21:32:15 -0600
Subject: SEM analysis of optical fibers

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---------------------------------------------------------------------------

Email: neetal.timble-at-cable.alcatel.com
Name: Neetal Timble

School: Clemson University


Question: I wanted to know whether there are any books available which
issues the topic of SEM analysis of optical fibers, which helps in
analyzing the SEM images of optical fibers for Break source Analysis.
Also I would like to know whether there are any other materials on this subject

---------------------------------------------------------------------------




From daemon Sat Feb 10 21:38:55 2001



From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Sat, 10 Feb 2001 21:20:12 -0600
Subject: onset of apoptosis in endothelial cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} } Hi,
} } I am trying to identify an onset of apoptosis in endothelial cells
(brain
} } tissue) using postembedding immunostaining method. So far I have tried 11
} } different AB's and have not
} } been able to stain the cells I know should be staining. Tissue has
} } already been embedded in lowacryl so i have no choice in the run up. Any
} } suggestions????
Neelima Shah..............
Biomedical Imaging Core Facility
Uni of Pennsylvania
Philadelphia, Pa.




http://www.MED.upenn.edu/morphlab/




From daemon Sat Feb 10 21:38:55 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Sat, 10 Feb 2001 21:19:52 -0600
Subject: Beta Probe?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings, listers!

Many years ago, probably during the mid '60's, I saw an instrument that was
described to me as a "Beta Probe". It was used in the lab of a company
selling non-ferrous metals (principally brasses and bronzes), and was an
analytical instrument which determined the composition of the products with
enough precision to qualify them for government specifications.

I remember parts of the demonstration I was given. A chunk of the material
was ground and polished, then simply set on an O-ring seal on the top of
the device. It was evacuated, the "Go" button was pushed, and after a
suitable time (and perhaps other manipulation) some counters were read.
Presumably these numbers were then converted, via a calibration, to the
composition.

At the time I had no clue what it was actually doing. Now I surmise that
it was an electron probe (probably with a "macro" spot size) with pre-set
crystal spectrometers.

If anyone knows anything more about this device, both I, and my aunt (who
managed the chemical part of the laboratory at that time - hence my visit!)
would be fascinated to know. Many thanks.

Tony.



* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Sat Feb 10 22:15:12 2001



From: puy85-at-icpc.ie (LUKE)
Date: Sat, 10 Feb 2001 22:13:22 -0600 (CST)
Subject: CONFIDENTIAL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


UNIVERSITY DIPLOMAS

Obtain a prosperous future, money earning power,
and the admiration of all.

Diplomas from prestigious non-accredited
universities based on your present knowledge
and life experience.

No required tests, classes, books, or interviews.

Bachelors, masters, MBA, and doctorate (PhD)
diplomas available in the field of your choice.

No one is turned down.

Confidentiality assured.

CALL NOW to receive your diploma
within days!!!

1-212-465-3248

Call 24 hours a day, 7 days a week, including
Sundays and holidays.




==============================
rem - techdept2001us-at-yahoo.com
==============================

824831130494379155526
yy-at-


From daemon Sat Feb 10 23:02:34 2001



From: joy43-at-icpc.ie (LUKE)
Date: Sat, 10 Feb 2001 22:59:34 -0600 (CST)
Subject: University Diploma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


UNIVERSITY DIPLOMAS

Obtain a prosperous future, money earning power,
and the admiration of all.

Diplomas from prestigious non-accredited
universities based on your present knowledge
and life experience.

No required tests, classes, books, or interviews.

Bachelors, masters, MBA, and doctorate (PhD)
diplomas available in the field of your choice.

No one is turned down.

Confidentiality assured.

CALL NOW to receive your diploma
within days!!!

1-212-465-3248

Call 24 hours a day, 7 days a week, including
Sundays and holidays.




==============================
rem - techdept2001us-at-yahoo.com
==============================

2038311679437204830


From daemon Sun Feb 11 03:25:16 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sun, 11 Feb 2001 20:19:59 +1100
Subject: Re: University Diploma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am member of few entomological listserver, even I am owner and moderator
of coleoptera listserver. What I do not understand why only this listserver
has spam messages. But When I try to write any message I am rejected..

Regards
Ricardo



From daemon Sun Feb 11 10:01:09 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sun, 11 Feb 2001 10:51:09 -0500
Subject: NYMS PLM Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Bernard Friedman Memorial Workshop

Polarized Light Microscopy
April 21, 28, May 5, 12, 2001

An advanced course on polarized light microscopy which will cover the
following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation
The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation

The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of polarized
light microscopy. The course instructors include Jan Hinsch of Leica, Inc.,
Mary McCann of McCann Imaging, John Reffner of Sensir Technologies and
N.Y.M.S. Instructor Don O'Leary.

WHEN: April 21, 28, May 5, 12, 2001 from 10 A.M. to 4 P.M.

WHERE: “Evergreens”, 30 N. Mountain Avenue, Montclair, NJ 07042
(accessible by public transportation, Information on car pools and
transportation will be provided.)

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the
Microscope" or are experienced in microscopy and familiar with the theory of
its use.

HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849

PLEASE POST
----------------------------------------------------------------------------
-------------------------------------
Registration Form
Polarized Light Microscopy

N.Y.M.S. Member_________________ ($275) Non-Member__________($295)

Name___________________________________________________________________
Address__________________________________________________________________
Phone (W)________________(H)______________________E-Mail___________________

Donald O'Leary
Curator & Education Chair
New York Microscopical Society
6 Chittenden Road
Fair Lawn, NJ 07410
(201) 797-8849



From daemon Sun Feb 11 14:00:56 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 11 Feb 2001 11:50:37 -0800
Subject: CD-ROMs for microscopy education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of Murphy's laws says that whenever something is published,
it's immediately out of date. Project MICRO's "Microscopy for Children: a
Bibliography", which was distributed with the December "Microscopy Today",
is no exception. I've been contacted recently about two CD-ROMs that will
interest many listserver readers. Since the web version of the
bibliography (URL below) won't be updated for a while, I'm providing
listings here:

Edmark Corp. 1998 Zap! $29.95, with a 50 page users' manual, from
Edmark at PO Box 97201, Redmond, WA 98073-9721, 800-320-8379,
www.edmark.com. For Macintosh or Windows 3.1 or 95.
Subtitled "Save the show with sound, light, and electricity", this
CD-ROM is sure to capture the interest of a computer game-addicted middle
schooler. All three topics are introduced in game format, complete with
levels of complexity and lots of shooting. In the optics section, laser
beams are aimed at mirrors, lenses, prisms, and filters; the targets are
eggs that hatch into cute monsters when activated by the beam. It actually
does a very good job of teaching reflection, refraction, absorption, and
color. The player who works through all levels of all three units then
can set up the light show for a concert, and even manipulate the sound.
It's much too time consuming to use in the classroom, but it's a delight
for home use. There is ample reference material, and homeschool parents
can set options to control complexity. Middle school. RECOMMENDED

Excalibur Mineral Company 1999 Photographic Guide to Mineral Species
$69.95+ $4.00 shipping from Excalibur at 1000 North Division SStreet,
Peekskill, NY 10566, www.bestweb.net/~excalmin. For Windows or Mac;
requires Netscape Communicator (included).
This is an "adult" CD, but the superb photos make it an excellent
visual supplement for a student who is learning about crystals and other
minerals. It's truly a "coffee table book" on a CD, with an unbelievable
5400 photos, which search and load rapidly in Netscape (and not at all in
Internet Explorer). There is an eye-catching 300 image automatic slide
show which can be set to play continuously. Images can be searched by
location or mineral content. They are mostly micromounts, photographed at
dissecting scope magnifications. Descriptions are minimal; it isn't
intended as a textbook substitute. Adult. RECOMMENDED

Want more? There are over 20 on the website.
Want copies of the bibliography for educational use? Contact me.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Feb 11 15:34:21 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Sun, 11 Feb 2001 22:29:43 +0100
Subject: Re: onset of apoptosis in endothelial cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Neelima,
wow, 11 different antibodies tested is a lot!
I don´t think that your problem is the choice of the antibody, you are enfaced with a more general problem.

Let´s assume your sections are about 60 nm thick.
This is about a 100 times thinner than a conventional paraffin section and about 200 times thinner than a conventional cryosection.
Thus, you have to consider that the amount of accesible epitopes/signal is 100-200 times weaker as seen in LM.
Then, your samples are already embedded in resin. I don´t know what kind of procedure you applied, but consider a factor } = 10 of epitopes destroyed during embedding.
That means at least 1000 times less epitopes in the same visible area as in LM-IHC. Impressive.

Now the other way round:
Let´s say a section of 1mm in square of (mouse) brain cortex contains about 50 capillaries and 3-4 greater vessels, the capillaries containing 2-3 endothelial cells hit. That makes altogether, mh, 200 endothelial cells in your section (not whole cells, only a 60 nm slice of them...).
I don´t know if you are examing normal tissue or pathological alterated (hypoxic, transgenic, inflamed, radiated etc...) but generally the apoptosis rate of adult continous endothelium is quite low.
Let´s say rate of apoptosis is 2%, that would mean that 3-4 cells in your 1mm in square area are apoptotic!
Common apoptosis markers refer to the nucleus. If 25% of all the endothelial cells show a nucleus hit by the section plane - expect one cell positive.
Even if apoptosis rate is 10% there would be optimistically 5 cells, including those who are in the holes of your section after the immunoprocedure :-(

In my point of view, you are looking for the needle in the haystack.

If I had to look for apoptotic cells by immunohistochemistry, I would take thick sections of mildly fixed tissue on a vibratome and do a discrete DAB-reaction-based IHC after the use of a detergent (e.g. AB against p18 of PARP). Then embed the sections with positive cells after osmification and focus on trimming your blocks towards the cells positive. (a pity that you have no choice in the run-up)

In the proceeded apoptosis of the cell you can observe typical changes in the nucleus and cytoplasm, so the use of an antibody is not the only way to look for apoptosis at EM level.

I´m curious what the other people on the listserver have to say to this topic.

I wish you good luck and cross my fingers for you.

Best regards,
Michael

Michael Reiner
University of Cologne
Dept. of Anatomy I
Germany


Neelima Shah wrote:
Hi,
I am trying to identify an onset of apoptosis in endothelial cells
(brain tissue) using postembedding immunostaining method. So far I have tried 11 different AB's and have not been able to stain the cells I know should be staining. Tissue has already been embedded in lowacryl so i have no choice in the run up. Any suggestions????

Neelima Shah
Biomedical Imaging Core Facility
Uni of Pennsylvania
Philadelphia, Pa.

_______________________________________________________________________________
Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de
Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de



From daemon Sun Feb 11 22:07:45 2001



From: Terry Robertson :      terryr-at-cyllene.uwa.edu.au
Date: Mon, 12 Feb 2001 12:00:35 +0800
Subject: Metal band for LKB Nova Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Urgent request
Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated

Terry



Dr Terry Robertson (PhD)
Senior Research Fellow
Department of Pathology
University of Western Australia
Nedlands 6009

Phone 618 9346 2935
Fax 618 9346 2891
Mobile phone 040302 5440
email terryr-at-cyllene.uwa.edu.au




From daemon Sun Feb 11 22:07:46 2001



From: Terry Robertson :      terryr-at-cyllene.uwa.edu.au
Date: Mon, 12 Feb 2001 11:57:50 +0800
Subject: Metal band for LKB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Urgent request
Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated

Terry


Dr Terry Robertson (PhD)
Senior Research Fellow
Department of Pathology
University of Western Australia
Nedlands 6009

Phone 618 9346 2935
Fax 618 9346 2891
Mobile phone 040302 5440
email terryr-at-cyllene.uwa.edu.au




From daemon Mon Feb 12 03:29:01 2001



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Mon, 12 Feb 2001 11:22:53 +0200
Subject: Photoshop purchase info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear everybody

I would like to purchase the Photoshop image software package. Unfortunately
our computer department does not have any information on the cost of this
package or where it can be purchased. Who can I contact for this information
?

Regards
W. Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]


From daemon Mon Feb 12 06:23:14 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 12 Feb 2001 12:15:14 +0000
Subject: Re: Metal band for LKB Nova Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Terry

Leica now handle LKB/Reichert microtome parts if you can still get them. I am trying to trace a part in the UK for myself so I will contact you if they come back with any information on your metal band, it may take two weeks
though. You could however try contacting Leica Australia see web address for details:
Main web page
http://www.leica-microsystems.com/
Australian website
http://www.leica-microsystems.com/cgi-bin/boxgate29910?login=guest&password=&opt=area%3DCompany

I get the impression that the normal electron microscope rule of at least 10 (maybe 15 if you're lucky) years of availability for spare parts won't apply to ultramicrotomes because of the major changes in the market about 12
or 13 years ago (in the UK: LKB ---} Reichert ---} Cambridge Instruments? ---} Leica).

Good luck

Malcolm

Malcolm Haswell
e.m. unit
University of Sunderland
UK

Terry Robertson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Urgent request
} Is there anyone out there in cyber space that may know where we can obtain the metal band (LKB part number 90-00-0047) that hold the specimen arm up on an LKB Nova ultramicrotome. Any help would be gratefully appreciated
}
} Terry
}
} Dr Terry Robertson (PhD)
} Senior Research Fellow
} Department of Pathology
} University of Western Australia
} Nedlands 6009
}
} Phone 618 9346 2935
} Fax 618 9346 2891
} Mobile phone 040302 5440
} email terryr-at-cyllene.uwa.edu.au



From daemon Mon Feb 12 08:14:40 2001



From: Louis Kerr :      lkerr-at-mbl.edu
Date: Mon, 12 Feb 2001 09:19:51 -0500
Subject: Summer 2000 Bio Microscopy Tech Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


2001 MICROSCOPY TECHNICIAN SUMMER POSITION AVAILABLE

The Marine Biological Laboratory has a summer position available for 10
to 15 weeks (June, July, and August) for a microscopy oriented
technician. We would like to attract someone with some knowledge of
biological preparative techniques and experience in any of the
following: laser scanning
confocal microscopy, TEM, SEM, and/or LM.

The technician will assist in the Central Microscopy Facility. The
technician's duties will be to check out incoming investigators in the
usage of our equipment and then to supervise its continuing usage and to
perform contract work for investigators. This may include fixation,
embedding, sectioning, scope use, darkroom work, etc. The technician will
also provide routine maintenance.

This is a short term and scientifically rewarding position. Salary will be
in the $8 to $10/hour range. Housing may be available to rent through MBL.

For more information, including a more detailed position description,
please contact Louis Kerr at: MBL, 7 MBL Street, Woods Hole, MA 02543.
Telephone, 508-289-7273; or at Email: lkerr-at-mbl.edu.

Please apply to: Human Resources, MBL, 7 MBL Street,
Woods Hole, MA 02543. or resume-at-mbl.edu.

You can visit our web site for online employment information and general
information regarding the Marine Biological Laboratory - http://www.mbl.edu/

An Equal Opportunity/Affirmative Action Employer/ Non-smoking workplace.


From daemon Mon Feb 12 08:53:29 2001



From: Lawrence Mason :      LMason-at-genetics.com
Date: Mon, 12 Feb 2001 09:49:30 -0500
Subject: TEM - Outsource sectioning/staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for a service (in the USA) where I can send my TEM blocks to be sectioned and stained (UA/LC). These are biological samples cured in Embed 812 resin.

Lawrence Mason
Research Scientist
Genetics Institute
lmason-at-genetics.com



From daemon Mon Feb 12 08:53:29 2001



From: Charlesworth, Jon :      charlesworth.jon-at-mayo.edu
Date: Mon, 12 Feb 2001 08:49:39 -0600
Subject: EM Tech Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mayo Clinic has an opening for an EM technician in the Muscle Research Lab
at the Rochester, MN campus. Details are as follows:

Research Technologist ñ Posting #01-0818

Summary:

Responsible for preparation of specimens for electron microscopy, evaluation
of specimens in electron microscope, and related photography work. Will
participate in research projects involving transmission EM, EM
cytochemistry, and immunocytochemistry; data analysis including morphometry;
assist in preparation of manuscripts for publication. Innovative and
creative individual capable of independent work with minimal supervision is
preferred. Executes experimental studies in support of lab goals, grant
commitments. Assists in the planning, design and modifications of
experiments. Performs basic statistical data analysis; prepares
tables/charts/graphs and assists in organizing data. May make presentations
and may contribute to writing of manuscripts. Trains
residents/fellows/temporary lab personnel in lab techniques. Maintains lab
equipment and supplies.

Qualifications:

Requires a Bachelor's degree in biology, chemistry, or other relevant
sciences. Experience required in lab projects/research work in educational
or work setting which would provide a solid understanding of lab techniques,
equipment, and safety. Must have the ability to organize and carry out
basic techniques independently. Candidate should have the ability to work
both independently and in a team. Experience with electron microscopy is
preferred.

For additional information on this position refer to posting # 01-0818 and
contact:

Jill M. Kelly
Staffing Specialist
Mayo Clinic - Ozmun East -4
kelly.jill-at-mayo.edu
ph #(507) 284-0510 fax# (507)284-9751
Assistant: Leah Carlson (507)284-3574



From daemon Mon Feb 12 11:33:57 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Mon, 12 Feb 2001 12:32:05 -0500
Subject: software for RDF of amorphous materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I am very interested in getting softwares to process electron diffraction
patterns from disordered materials to obtain the G(r) (reduced intensity
function), and J(r) (radial distribution function).

Any information on how to get these softwares is appreciated. In
particular, information on free ones are most welcome.

Regards
Yan Xin

=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Mon Feb 12 12:53:39 2001



From: Michael B. Ferrari :      ferrari-at-uark.edu
Date: Tue, 13 Feb 2001 00:41:50 +0800
Subject: Cell Physiol Postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral position in Cell Physiology

Qualified candidates (Ph.D. or other terminal degree) are sought for a
NIH-funded
postdoctoral position to study intracellular calcium signals and their
downstream
effectors which regulate myofibril assembly. Primary training in real-time
fluorescence digital microscopy (both conventional and confocal) using
calcium tools
and fluorescently tagged sarcomeric proteins expressed or microinjected in
cultured
myocytes. Prior training in protein biochemistry or molecular biology
highly desirable. Interests and skill areas could include: cell signaling,
cytoskeleton, actin or myosin dynamics, intracellular calcium channels,
muscle development. Salary and start date are negotiable, funding available
summer 2001. Please reply offline. See website below for more info.

Mike


Michael B. Ferrari
Department of Biological Sciences
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-5349
http://biology.uark.edu/ferrari




From daemon Mon Feb 12 17:25:47 2001



From: Terry Robertson :      terryr-at-cyllene.uwa.edu.au
Date: Tue, 13 Feb 2001 07:14:03 +0800
Subject: LM Complement antibodies that work on paraffin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone out there in immuno cyber space had any experience in demonstrating complement C3 or C5 on formalin fixed frozen or paraffin sections. I need a good antibody that works. Any comments would be gratefully received.

Terry




Dr Terry Robertson (PhD)
Senior Research Fellow
Department of Pathology
University of Western Australia
Nedlands 6009

Phone 618 9346 2935
Fax 618 9346 2891
Mobile phone 040302 5440
email terryr-at-cyllene.uwa.edu.au




From daemon Mon Feb 12 18:43:51 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 12 Feb 2001 16:38:36 -0800
Subject: TEM-Resins-Save or Discard?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Someone from another lab that used to do EM, but doesn't do EM any more
just delivered a couple of boxes of old resin components to me to 'take
care of'.

It was OK with me, being the EM guy I should know what to do with them, but
I realized I wasn't sure. I already had a bunch of old resins left over
from kits folks had bought through the years and I figured I could just add
it to the pile, but now there is a space problem, too may old bottles and
not enough space to keep them.

These things are old, 1980's, but could they still be good enough to use?
Is it worth finding space for them in case some poor starving student comes
along who can't afford new supplies but is dying to do EM? What is the
shelf life of things like this?

What would you do? Mix up a bunch of paper weights? Call EH&S and have them
take away the bottles of individual components? Consolidate the nearly
empty bottles to save space? Would you ever use things this old, I don't
think I would. I am tempted to just get rid of them in one way or another.
But I am haunted by the possibility that I will be tossing perfectly good
stuff.

Relieve me of my guilt, tell me what to do.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Feb 12 22:08:31 2001



From: John Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Tue, 13 Feb 2001 14:03:57 +1000
Subject: Re: TEM-Resins-Save or Discard?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don't waste your time, disgard the lot.
Regards
JVN

Jon Krupp wrote:
}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi:
}
} Someone from another lab that used to do EM, but doesn't do EM any more
} just delivered a couple of boxes of old resin components to me to 'take
} care of'.
}
} It was OK with me, being the EM guy I should know what to do with them, but
} I realized I wasn't sure. I already had a bunch of old resins left over
} from kits folks had bought through the years and I figured I could just add
} it to the pile, but now there is a space problem, too may old bottles and
} not enough space to keep them.
}
} These things are old, 1980's, but could they still be good enough to use?
} Is it worth finding space for them in case some poor starving student comes
} along who can't afford new supplies but is dying to do EM? What is the
} shelf life of things like this?
}
} What would you do? Mix up a bunch of paper weights? Call EH&S and have them
} take away the bottles of individual components? Consolidate the nearly
} empty bottles to save space? Would you ever use things this old, I don't
} think I would. I am tempted to just get rid of them in one way or another.
} But I am haunted by the possibility that I will be tossing perfectly good
} stuff.
}
} Relieve me of my guilt, tell me what to do.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Tue Feb 13 05:54:01 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 13 Feb 2001 06:47:56 -0500
Subject: Re:job offer from RangeTS@aol.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Job Hunters,
There was a job offer on the server last month from RangeTS-at-aol.com for
an SEM tech job in Florida. Did anyone send any info and, if so, did
anyone get a reply? Concerns have been expressed to me about having
sent off personal info to a blind add and then getting no reply.

If RangeTS-at-aol.com is lurking out there, please have the courtesy to
reply to all who answered your add ASAP. Taking personal info without
acknowledgement or info on who you are is rather unprofessional. Blind
adds are not the best way to go, but if you feel you must use them, the
good folks on this listserver expect a professional response. Thank you.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA 17314



From daemon Tue Feb 13 07:20:23 2001



From: FARNHAM, WARREN H :      WHFARN-at-solutia.com
Date: Tue, 13 Feb 2001 08:11:00 -0500
Subject: TEM: Ultramicrotomy of Emulsion PSA

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Greetings;

I am interested in characterizing emulsion latex Pressure Sensitive
Adhesives (PSA) in their aqueous phase state and as cast films. Can
anyone offer any specific techniques tips for doing cryo-
ultramicrotomy of such material? Are there any good literature
references that would be informative? Any recomendations for staining
techniques of Acrylic based copolymer based adhesives?

Warren


From daemon Tue Feb 13 08:26:12 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 13 Feb 2001 08:20:50 -0600
Subject: Throwing away old stuff

Contents Retrieved from Microscopy Listserver Archives
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At 04:38 PM 2/12/01 -0800, Jon Krupp wrote:
} Someone from another lab that used to do EM, but doesn't do EM any more
} just delivered a couple of boxes of old resin components to me to 'take
} care of'.

I wholeheartedly encourage this kind of behavior... Tell the
mailing list about old stuff you're about to throw away!

Someone out there might be willing to pay the cost of shipping
and handling to get it, and perhaps they'll put it to good use.

It may not need to be fresh, contemporary or professional quality;
don't forget the schools, teachers, amateurs, etc.

- John



From daemon Tue Feb 13 09:34:08 2001



From: Jay Campbell :      jmcampbe-at-facstaff.wisc.edu
Date: Tue, 13 Feb 2001 09:29:57 -0600
Subject: Microtubule Preservation

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Hello All,
Can anyone suggest/recommend a fixation protocol that preserves
microtubules and/or centrioles without needlessly extracting other
cellular components?

Thanks for any response,
Jay Campbell
University of Wisconsin
Microscopy Resource


From daemon Tue Feb 13 09:43:23 2001



From: David_Bell-at-millipore.com
Date: Tue, 13 Feb 2001 10:46:31 -0500
Subject: Re:job offer from RangeTS@aol.com

Contents Retrieved from Microscopy Listserver Archives
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Hi Ken,

I sent some of the requested info, but I held some back just in case the
request was bogus. It seems my fears are justified, as I never received a
reply. On the other hand, I have not received any new spam, so perhaps
it's not a diabolical plot. ;-)

I do hope, if it was a legitimate offer, that RangeTS-at-aol.com will take the
time to respond to those who expressed an interest.



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108





Ken Converse
{qualityimages-at-n To: "MSA, listserver" {Microscopy-at-sparc5.microscopy.com}
etrax.net} cc:
Subject: Re:job offer from RangeTS-at-aol.com
02/13/01 06:47
AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Job Hunters,
There was a job offer on the server last month from RangeTS-at-aol.com for
an SEM tech job in Florida. Did anyone send any info and, if so, did
anyone get a reply? Concerns have been expressed to me about having
sent off personal info to a blind add and then getting no reply.

If RangeTS-at-aol.com is lurking out there, please have the courtesy to
reply to all who answered your add ASAP. Taking personal info without
acknowledgement or info on who you are is rather unprofessional. Blind
adds are not the best way to go, but if you feel you must use them, the
good folks on this listserver expect a professional response. Thank you.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA 17314







From daemon Tue Feb 13 11:34:14 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 13 Feb 2001 12:28:54 -0500
Subject: Acrylic PSA adhesives by TEM

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Warren wrote the following:
=======================================================
I am interested in characterizing emulsion latex Pressure Sensitive
Adhesives (PSA) in their aqueous phase state and as cast films. Can
anyone offer any specific techniques tips for doing cryo-
ultramicrotomy of such material? Are there any good literature
references that would be informative? Any recomendations for staining
techniques of Acrylic based copolymer based adhesives?
=======================================================
This is not a question that is easily answered because you have to state
further just what are your analytical objectives for wanting to do this work
in the first place.

But if a typical (some are not "typical") acrylic emulsion, then the
"particles" have to be "hardened" up in some way, otherwise they will
coalesce and pancake, and when viewed by TEM will, otherwise, be one big
ambiguous mess. So the soft particles have to be hardened up (into what
look like hard rigid "marbles") and that is generally done using UV
irradiation in quartz tubes or sometimes using RuO4. We get the best
results on these kinds of emulsions with samples lightly shadowed with Pt/C.

For the sectioning of the resulting adhesive film, again this is a TEM job,
you have to define whether you want to look at the interface with the
release paper or some other characteristics, such as the dispersion of
inorganic additives in the adhesive coating. Evidence of the release layer
can sometimes be resolved this way as well. The preparation methods are
different (for the different objectives) but the common feature is that they
all must be cryo-sectioning using a good state of the art cryo-
ultramicrotome and using only good quality diamond knives. Anyone who has
either tried or done this knows that it can take one quite a long time to
develop the magic of the "art" before good sections can be made on a
reproducible basis.

I would be happy to give you more information off-line or better yet, our
laboratory services division would be very happy to give you a quote to do
the work for you.

Disclaimer: Our firm manufactures and distributes the kinds of materials
and supplies needed for the conduct of this type of work and when one
prefers to send it to an outside laboratory, we also perform studies on
these kinds of samples for clients in our own facilities.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Tue Feb 13 12:44:52 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 13 Feb 2001 13:42:42 -0500
Subject: Re: Microtubule Preservation

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Jay Campbell wrote:

} Hello All,
} Can anyone suggest/recommend a fixation protocol that preserves
} microtubules and/or centrioles without needlessly extracting other
} cellular components?
}
} Thanks for any response,
} Jay Campbell
} University of Wisconsin
} Microscopy Resource

You might have a look at "Increased visualization of microtubules by an
improved fixation method". Luftig, R. B. et al. J. Histochem. Cytochem.
25:175-187, 1977.
I seem to recall someone using PIPES as a buffer for glut fixation to
improve microtubules.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Feb 13 12:46:58 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 13 Feb 2001 12:43:45 -0600
Subject: Re: Microtubule Preservation

Contents Retrieved from Microscopy Listserver Archives
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Hi Jay,
You didn't say what kind of cells so I won't comment
specifically. But in general a good fix for tubes will have 10 mM
EGTA to chelate calcium. A typical fix might contain 4 %
paraformaldehyde, 0.1% glut, 25 mM Pipes pH 7.0, 10 mM EGTA, 1 mM
MgSO4. Remember to fix at room temp, at least for the first half
hour or so, because microtubules are cold sensitive.
Hope this helps,
Tobias

}
}
}
} Hello All,
} Can anyone suggest/recommend a fixation protocol that preserves
} microtubules and/or centrioles without needlessly extracting other
} cellular components?
}
} Thanks for any response,
} Jay Campbell
} University of Wisconsin
} Microscopy Resource

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Tue Feb 13 13:17:26 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 13 Feb 2001 14:13:41 -0500
Subject: RE:TEM outsource sectioning and staining

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I have extensive experience in all types of biological specimens and TEM.
If you would like to discuss your project and estimated costs, please
contact me at 716-275-1954. Our EM laboratory is housed in the Pathology
Department, but serves as a core facility to any University of Rochester
Med. Ctr. researchers. We also do projects for companies and researchers
outside the University. So please call if you are interested in a
discussion...

Sincerely,

Karen L. Jensen, M.S.
Project Manager & Associate Scientist
Electron Microscopy Research Core, Box 626
University of Rochester Medical Center
Rochester, NY 14642




From daemon Tue Feb 13 13:18:39 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 13 Feb 2001 14:15:37 -0500
Subject: RE:TEM resins-Save or Discard

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I disagree with JVN, because if the bottles have never been opened
then they can definitely be used. Other EM technicians I have surveyed here
at the University of Rochester Medical Center in Rochester, NY have used
resins purchased in the 1980's in the 1990's-2000. So, if they are resins
that you routinely order, start using the older bottles. Save yourself some
money!
Also you can test embedd a specimen. By the way if any of the
resins are Lowicryls, they are definitely OK unless they have turned color,
should be white not pale yellow. Also, here at the U of R we have a
chemical recycle list that is accessible to other researchers here, thereby
providing an opportunity to use unopened resins, chemicals, etc...Maybe your
university has a similar program.


Karen L. Jensen, M.S.
Project Manager & Associate Scientist
Electron Microscopy Research Core




From daemon Tue Feb 13 13:31:51 2001



From: Christoph Wittig :      Christoph.Wittig-at-iw.uni-halle.de
Date: Tue, 13 Feb 2001 20:29:20 MET
Subject: Re: TEM-Preparation of dispersion samples

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Petra Volfova wrote:
}
} I would like to request of preparation samples from
} poly(styrene)/poly(butyl acrylate) dispersion with 100 nm particles size
} for measurements by JEOL type of transmission electron microscope.
} We have got some problems with dilution of latexes and with time of
} staining of carbon, too. We would like to confirm a core/shell
} particles morphology of samples prepared by two step seeded emulsion
} polymerization. We have got some problems with magnification and contrast
} expansion of core/shell particles,too.
} Thank you very much for your help.
}
} Petra Volfova, PhD. student of Department of plastics and rubber,
} Slovak Technical University,Bratislava,Slovak Republic.
}

Dear Petra,
Below are my experiences with TEM on polystyrene/poly(butyl
acrylate) latexes. Please note that I'm not a specialist in this
area and these are _not_ 100% perfect receipts - but it often
works for me.
Dilution: Wet the end (1...2cm) of a glass stick with your latex
and dip it in a test tube filled with ca. 10 ml dist. water.
The water must become slightly opaque. Repeat one or two
times (depending from the concentration of the latex).
Staining: If poly(butyl acrylate) forms the shell, some negativ
staining could be helpful, because the acrylat phase itself is
often not visible in TEM. I used uranyl acetate, two drops of
2% UA solution in 10 ml diluted latex. The PS-phase can be
stained by vapour staining with Ruthenium (0.1g RuCl3 + 5 ml
Na-Hypochlorid solution (13% Cl)).
Preparation: I've mounted copper grids, coated with a 10 nm carbon
film, on a specimen holder (from a TESLA BS500 TEM) and placed
a drop diluted latex on the grid (formvar or collodium coated grids
are not recommendable, because these films are very unstable under
the electron beam after Ru-staining). After drying (some hours at
room temp.) the grid can be umounted for vapour staining.
If you have an "ultrasonic fogger" (or how this device is called
in english, in german it's an "Ultraschallvernebler"), you can
use it with very good results for preparation of latices smaller
than ca. 200 nm.
Microscopy: There is nothing special, if you have problems with the
contrast, play a little bit with the accelerating voltage and
objective aperture. Magnification should be not the problem.
In polystyrene-core/poly(butyl acrylate)-shell particles the
core/shell structure should be visible (if the acrylate shell is
not too thin). If PS forms the shell, I've never seen a core/shell
structure. In this case, you must embed the dryed latex particles
in a resin (acrylate or epoxy), cut ultrathin sections and stain
them for visualization of the core/shell structure (if the particles
are not crosslinked, they may be soluble in some kind of resin,
especially at higher temperatures).

Literature, that may help:
A.W. Robards, A.J. Wilson (editors): Procedures in Electron Microscopy;
John Wiley and Sons, 1993-1998 (a comprehensive collection of receipts,
eg. staining methods, preparation of carbon and formvar coated grids etc.)

J.S. Trent, J.I. Scheinbeim, P.R. Coachman, Macromolecules
16(1983)589-598 (Ru staining)


Good luck,

Christoph



From daemon Tue Feb 13 13:42:47 2001



From: Judy Trogadis :      judy-at-uhnres.utoronto.ca
Date: Tue, 13 Feb 2001 14:38:17 -0800
Subject: job ad

Contents Retrieved from Microscopy Listserver Archives
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I am posting this for a friend of mine:


Rod Bremner wrote:

Technician
Immunohistochemistry/Histology/Molecular biology

A technical position is available at the Eye Research Institute, Toronto Western Research Institute, University Health Network. Experience in immunohistochemistry and histology is essential, and a background in molecular biology would be beneficial. The position can either be a full time junior post, or part time senior post. The successful candidate will assist investigators who study the molecular mechanisms of eye development and disease. A CV and the names/phone numbers of two referees should be sent to Rod Bremner,

email: rbremner-at-uhnres.utoronto.ca,
Fax: 416 603 5126.



Judy Trogadis
Vision Science Research Program
Toronto Western Research Institute
399 Bathurst St.
Toronto, ON M5T 2S8, Canada
ph: 416-603-5088
fax: 416-603-5126
email:judy-at-uhnres.utoronto.ca




From daemon Tue Feb 13 14:29:05 2001



From: FERGMANN-at-aol.com
Date: Tue, 13 Feb 2001 15:23:50 EST
Subject: Forensic Microscopy Course

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Dear Fellow Microscopists...I'm looking for suggestions on how to plan an
undergraduate forensic microscopy course. Chances are the course will have
to start with a generic introduction to microscopy, so anything on general
undergraduate microscopy would also be appreciated. Books? Demonstrative
Aids? Personal experiences with undergrad instruction, Etc. Any and all
help would be appreciated. Thanks in advance for your time! Mary-Jacque Mann


From daemon Tue Feb 13 16:37:45 2001



From: Gramp Skin Pathology :      grampath-at-camtech.net.au
Date: Wed, 14 Feb 2001 09:04:33 +1030
Subject: CD34 Thanks

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Thanks to all the people who replied to my posting about CD34.

I’m still working on it !

Mark




From daemon Tue Feb 13 16:38:39 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Wed, 14 Feb 2001 09:39:30 +1000
Subject: Re: onset of apoptosis in endothelial cells

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Hi Neelima,

One further comment after Michael's excellent summary.

If you can see any apoptotic cells in the sections, you shoud try and
label these same cells using neighbouring sections. If these don't
stain, you have a problem. You might also try and beg/borrow a
Lowicryl embedded sample known to have reacted positively for an
apoptotic marker, so that at least you can see if your antibody
works. But really, apoptosis is such a rapid event that
ultrastructural ID is perhaps as good as labelling, depending of
course on why you want to label them. I seem to remember that
apoptosis is complete in 20 mins. Shoot me down if I'm wrong!

Diana
--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Wed Feb 14 02:31:46 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Wed, 14 Feb 2001 09:27:36 +0100
Subject: Re: Microtubule Preservation

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Jay,
Sorry, I forgot one thing!
Tobias Baskin´s proposal for a MT fix sounds good as well. EGTA seems to me useful.
But anyway, what kind of fixative you will choose, DON`T forget to keep your fixative at room temperature at least, this is a crucial prerequisite.

Michael
______________________________________________________________________________
Die Fachpresse ist sich einig: WEB.DE 18mal Testsieger! Kostenlos E-Mail,
Fax, SMS, Verschlüsselung, POP3, WAP....testen Sie uns! http://freemail.web.de



From daemon Wed Feb 14 02:31:46 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Wed, 14 Feb 2001 09:22:49 +0100
Subject: Re: Microtubule Preservation

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Hello Jay!
Try it with a fixative containing glut and tannic acid (TA), followed by osmification and embedding in epoxy.
There is a classical paper:

Lewis G. Tilney et al.:
"Microtubules: Evidence for 13 Protofilaments", JCB 59,pp 267-275, 1973

Tilney used 2% Glut and 2-8% tannic acid made in phosphate buffer. 1% digitonin was added to faciitate TA penetration.

My recipe is the following:
make a sticky TA solution:
solve 25 grs TA powder in 25 ml aqua, heat on a stirrer until you have a dark brown solution, be quick, so it will remain fluid. Filter with a paper filter . The solution made is stable over years, the older it gets the better your result.
For fixation add 1-2% v/v of the stock TA solution to a cacodylate/phosphate buffer containing 4% Formaldehyd and 2% Glutaraldehyde. May be you will have to add 0,5-1% digitonin, but TA from the stock solution penetrates not that bad as directly in the fix soluted TA powder will do.
Don´t fix your samples too long, because overcontrasting can occur (max. 24 hours).
Then, follow a standard embedding protocol including osmificartion and uranylic acetate en-bloc staining. Try a quick dehydration with aceton to avoid tissue component extraction but I have satisfactorily results with normal alchoholic dehydration.

Hope this helps!

Good luck,
with best regards,
Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I

Jay wrote
} Hello All,
} Can anyone suggest/recommend a fixation protocol that preserves
} microtubules and/or centrioles without needlessly extracting other
} cellular components?

______________________________________________________________________________
Die Fachpresse ist sich einig: WEB.DE 18mal Testsieger! Kostenlos E-Mail,
Fax, SMS, Verschlüsselung, POP3, WAP....testen Sie uns! http://freemail.web.de



From daemon Wed Feb 14 09:19:43 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Wed, 14 Feb 2001 09:43:55 -0500
Subject: APOPTOSIS

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To the person looking for ultrastructural evidence of apoptosis: Take a
look at one of my papers: American Journal of Pathology, Vol. 156, No. 6,
June 2000. Good Luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Wed Feb 14 11:40:41 2001



From: csedax-at-alpha.arcride.edu.ar
Date: Wed, 14 Feb 2001 14:38:07 -0300
Subject: SEM: Asbestos, to have or not to have?

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Hi dear microscopists,

here we are, trying to find out how to face a SEM
work related to a request from someone who manufactures some
kind of synthetic boards to build up ceilings. We do not know much
about this product but, they ensure it does not contain asbestos.
Actually, they need to proof that!.

How would you make up the measurements in order to garantee or
proof that no asbestos at all are found in these boards. It means a
kind of "negative experiment" what we are asked to do.

Any experiences or sugestions?. We are looking at standards or
regulations but not found about the sampling and measuring
(observation) procedure for a negative result.

Thanks in advance,

Silvia Montoro
Centro Regional de Investigación y Desarrollo de Santa Fe
Güemes 3450
3000 Santa Fe
Fax +54 - 342 - 455 0944
e-mail: csedax-at-arcride.edu.ar
smontoro-at-arcride.edu.ar
npratta-at-arcride.edu.ar


From daemon Wed Feb 14 11:40:41 2001



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Wed, 14 Feb 2001 11:31:41 -0800
Subject: cryoSEM

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I was recently asked who manufactures cryo stage/prep equipment for
SEM's. We have had such equipment for several years, but I have no idea
what other suppliers are out there. Any help?
Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Wed Feb 14 12:32:41 2001



From: George Lawton :      George.Lawton-at-UTSouthwestern.edu
Date: Wed, 14 Feb 2001 12:27:58 -0600
Subject: Thanks for EDS replies

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all the contributors of the Listserver

I want to say thanks to all people who replied to my inquiry on who can do EDS. I received 17 responses and/or suggestions. I was overwhelmed with the response. I have passed the information on to the investigator who will decide what direction she wants to go in.

I can't tell you how many times the listserver has come to the recuse. Sometimes a response comes while I'm thinking about asking the listserver. It like you are reading my mind. Besides being a lifesaver, it is also educational. I learn alot from all of you.
Thanks again.

George

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
Fax 214-648-6408
eMail: George.Lawton-at-UTSouthwestern.edu


From daemon Wed Feb 14 15:21:13 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Wed, 14 Feb 2001 16:13:59 -0500
Subject: Dual Ion Mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I posted a message to say that we have a Gatan Dual Ion Mill to dispose
of. We have been swamped with replies. I had no idea what I was letting
myself in for. Moreover, I got flu and so have been unable, til now, to
respond. So, please no new contacts. Also those who have made contact
already, please be patient. I will get to deciding what to do as soon
as I can.

--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Wed Feb 14 17:55:23 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 15 Feb 2001 09:51:34 +1000
Subject: RE: cryoSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Emitech makes several models. You can read about them in our on online:
Home} Contents} K4
Disclaimer: ProSciTech supplies these instruments, but only within Australasia
south of Singapore.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, February 15, 2001 5:32 AM, Bill Chissoe [SMTP:wchiss-at-ou.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was recently asked who manufactures cryo stage/prep equipment for
} SEM's. We have had such equipment for several years, but I have no idea
} what other suppliers are out there. Any help?
} Bill
}
} --
} =============================================================
} Bill Chissoe III
} Electron Microscopist
} University of Oklahoma
} 770 Van Vleet Oval
} Norman, Ok. 73019
} E-mail: wchiss-at-ou.edu Ph. (405)325-4391
} =============================================================
}
}



From daemon Wed Feb 14 19:42:37 2001



From: JENKINS-at-afip.osd.mil
Date: Wed, 14 Feb 2001 19:34:01 -0600
Subject: TEM of FECAL Material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone out there have experience in cleaning up fecal material so it
can be processed for TEM. We are looking for a virus which we are going to
label. Any help, anyone could give me ASAP would be greatly appreciated

Marie Jenkins
Lab Manager
Department of Environmental & Toxicologic Pathology
Armed Forces Institute of Pathology Bldg 54 Rm M093B
Washington, D.C. 20306-6000
Tel:202-782-2734
Fax: 202-782-9215
jenkins-at-afip.osd.mil




From daemon Wed Feb 14 20:39:07 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Tue, 13 Feb 2001 11:33:32 +1300
Subject: Information on old books (Light Microscopy)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a rationalisation of our institutes library we have come across
two old volumes of the Illustrated Annual of Microscopy dated 1898
and 1900 published by Percy Lund, Humphries & Co London. Has
anyone any information on the scarcity of these volumes so we can
decide whether to donate them to a library with better archival
storage facilities.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


From daemon Thu Feb 15 03:53:07 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Thu, 15 Feb 2001 11:27:35 +0000 (GMT)
Subject: Re: SEM: Asbestos, to have or not to have?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gatan (incorporating the cryo EM bit of Oxford Instruments), VG
Microtech (Polaron), EmiTech and Bal-Tech are main players.
Their contact details can be found in the Microscopy Vendors
Database

http://www.kaker.com/mvd/list.html
Chris

Date sent: Wed, 14 Feb 2001 11:31:41 -0800
} From: Bill Chissoe {wchiss-at-ou.edu}


We often get dust and bits of board from the site here to examine. We use powder X-ray diffraction to test for asbestos. If you have a theta-2theta diffractometer you could use, I can give you a protocol.

Richard

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi dear microscopists,
}
} here we are, trying to find out how to face a SEM
} work related to a request from someone who manufactures some
} kind of synthetic boards to build up ceilings. We do not know much
} about this product but, they ensure it does not contain asbestos.
} Actually, they need to proof that!.
}
} How would you make up the measurements in order to guarantee or
} proof that no asbestos at all are found in these boards. It means a
} kind of "negative experiment" what we are asked to do.
}
} Any experiences or suggestions?. We are looking at standards or
} regulations but not found about the sampling and measuring
} (observation) procedure for a negative result.
}
} Thanks in advance,
}
} Silvia Montoro
} Centro Regional de Investigación y Desarrollo de Santa Fe
} Güemes 3450
} 3000 Santa Fe
} Fax +54 - 342 - 455 0944
} e-mail: csedax-at-arcride.edu.ar
} smontoro-at-arcride.edu.ar
} npratta-at-arcride.edu.ar

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Thu Feb 15 06:31:45 2001



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Thu, 15 Feb 2001 13:37:50 +0100 (CAT)
Subject: Coater Unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Everybody,
Our central science lab is trying to egt a coater unit, can any body
suggest where to get one and what are the performance xteristics that goes
with it also all neccesary accessories.
thank You

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.

email: sojilori-at-oauife.edu.ng



From daemon Thu Feb 15 07:19:18 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 15 Feb 2001 08:13:18 -0500
Subject: Re: cryoSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
"message to: MSA list" {microscopy-at-sparc5.microscopy.com}
X-Mailer: QuickMail Pro 2.1 (Mac)
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From: Debby Sherman :      dsherman-at-purdue.edu
Date: 15 Feb 2001 08:13:18 -0500
Subject: Re: cryoSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Bill,
Gatan has recently taken over the SEM cryostage previously marketed by Oxford Instruments (originally owned by Hexland in the 80's). My understanding is that the deal was the division in it's entirety which means that the personnel involved with tech support, sales, etc. also moved on to Gatan.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907


On Wednesday, February 14, 2001 2:31 PM, Bill Chissoe {wchiss-at-ou.edu} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Feb 15 08:57:18 2001



From: JHumenansky-at-phi.com
Date: Thu, 15 Feb 2001 08:49:08 -0600
Subject: Re: SEM: Asbestos, to have or not to have?

Contents Retrieved from Microscopy Listserver Archives
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To analyze for asbestos at least two approaches can be used. A small piece
of the sample can be pulverized and characterized by PLM. A more complete
method that will provide some quantitative measure is to pulverize a
measured mass of the material and then put into a DI solution and sonicate.
Aliquot of the solution are then redeposited onto special filters via
vacuum filtration and then prepped for analysis by TEM/EDS. There are many
labs in the USA that specialize in these types of analysis. You can
contact them to get more detailed information on these types of analysis.

Hope this helps.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Thu Feb 15 09:53:41 2001



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Thu, 15 Feb 2001 10:46:43 -0500
Subject: SEM: Asbestos, to have or not to have?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In America, there are lots of commercial asbestos testing labs. The analysis
is usually performed by utilizing a TEM and/or a PLM based on protocols by
NVLAP or other agencies. In your case, the most reliable +/- confirmation
can be done with a TEM in 10 minutes, including sample preparation, should
you have the permission to do asbestos analysis and be familiar with the
morphology/structure(SAED)/chemistry(EDS). But I would like to suggest you
send the sample out for the analysis because of the HAZMAT issue. It costs
$60-$200 for one sample. For further information, you can contact me off
line.

Disclaimer: I'm not doing asbestos analysis any more though had done it for
a living for a couple of years, and nor do I have any financial interests in
this kind of analysis.

-cy, Scientist, Rodel Inc.


-----Original Message-----
} From: "csedax-at-alpha.arcride.edu.ar"-at-sparc5.microscopy.com
[mailto:"csedax-at-alpha.arcride.edu.ar"-at-sparc5.microscopy.com]
Sent: Wednesday, February 14, 2001 12:38 PM
To: Microscopy-at-sparc5.microscopy.com
Cc: csedax-at-ARCRIDE.EDU.AR


Hi dear microscopists,

here we are, trying to find out how to face a SEM
work related to a request from someone who manufactures some
kind of synthetic boards to build up ceilings. We do not know much
about this product but, they ensure it does not contain asbestos.
Actually, they need to proof that!.

How would you make up the measurements in order to garantee or
proof that no asbestos at all are found in these boards. It means a
kind of "negative experiment" what we are asked to do.

Any experiences or sugestions?. We are looking at standards or
regulations but not found about the sampling and measuring
(observation) procedure for a negative result.

Thanks in advance,

Silvia Montoro
Centro Regional de Investigación y Desarrollo de Santa Fe
Güemes 3450
3000 Santa Fe
Fax +54 - 342 - 455 0944
e-mail: csedax-at-arcride.edu.ar
smontoro-at-arcride.edu.ar
npratta-at-arcride.edu.ar


From daemon Thu Feb 15 10:27:43 2001



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 15 Feb 2001 10:19:46 -0800
Subject: Thanks:re. cryoSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who answered my inquiry about cryoSEM. As always, this is
a very friendly, helpful group.
Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Thu Feb 15 11:38:36 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Thu, 15 Feb 2001 12:32:42 -0500
Subject: RE: SEM ASBESTOS TO HAVE OR NOT TO HAVE?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is my understanding that all asbestos testing with an Electron
Microscopy Lab must acquire certification from not only their state health
agency but also there are Environmental Protection Agency regulations, one
must thoroughly examine. I would be very cautious to accept any work
involving Asbestos testing. Several laboratories have been sued.
We were also approached by a local company in Rochester, NY who
would provide us with a substantial amount of money to test asbestos, but
upon talking to the NY state Health Dept., and looking into the Technician's
certification course(which is not cheap and is 1year), and the laboratory
would also have to be licensed, we decided it wasn' t going to be worth it.

Karen L. Jensen, M.S.
Project Manager & Associate Scientist
Electron Microscopy Research Core




From daemon Thu Feb 15 12:01:41 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Thu, 15 Feb 2001 12:56:51 -0500
Subject: TEM of FECAL Material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


STOOL Samples of about 2-4 gm can be collected into a sterile
container filled only half way. Make a 10-20% suspension in distilled water
and clarify by centrifuging at 3000 rpm for 30 minutes.
Then take the supernatant and centrifuge again at 15,000 RPM for one
hour to concentrate a viral laden pellet. This pellet you could resuspend
in various dilutions and drop onto a coated grid and immunolabel.


Karen L. Jensen, M.S.
Project Manager & Associate Scientist
Electron Microscopy Research Core



-----Original Message-----
} From: "JENKINS-at-afip.osd.mil"-at-sparc5.microscopy.com
[mailto:"JENKINS-at-afip.osd.mil"-at-sparc5.microscopy.com]
Sent: Wednesday, February 14, 2001 8:34 PM
To: microscopy-at-sparc5.microscopy.com


Does anyone out there have experience in cleaning up fecal material so it
can be processed for TEM. We are looking for a virus which we are going to
label. Any help, anyone could give me ASAP would be greatly appreciated

Marie Jenkins
Lab Manager
Department of Environmental & Toxicologic Pathology
Armed Forces Institute of Pathology Bldg 54 Rm M093B
Washington, D.C. 20306-6000
Tel:202-782-2734
Fax: 202-782-9215
jenkins-at-afip.osd.mil




From daemon Thu Feb 15 15:13:00 2001



From: Bob Price :      price-at-dcsmserver.med.sc.edu
Date: Thu, 15 Feb 2001 16:03:54 -0500
Subject: M&M 2001 Paper submission deadline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listmembers,

The official deadline for submission of papers for the 2001 Microscopy
and Microanalysis meeting is today, February 15. However, there have
been some problems with the database so the deadline is being extended
until Noon Eastern Time on Monday, February 19. All Title, Author and AV
requirement data must be entered into the database at
mmconference.org/2001 by that time and papers must be received at the
following address by Tuesday, February 20.

MSA Meeting Managers
7000 West Southwest Highway
Chicago Ridge, IL 60415

877-672-6271 (Telephone)

If you have problems submitting your Author information, or if you would
like to submit a paper for the late breaking poster session after February
19, please contact Bob Price (Price-at-med.sc.edu) or 803-733-3393

Thanks.

Bob
Bob Price
M&M 2001 Program Chair
803-733-3393 (T)
803-733-1533 (F)


From daemon Thu Feb 15 16:31:19 2001



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Thu, 15 Feb 2001 16:20:12 -0600
Subject: job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Manager Keck Center for Characterization of Nanoscale Soft
Materials-Northwestern University seeks a Ph.D. scientist with experience in
operation of imaging TOF-SIMS, high vacuum STM/AFM and high throughput XPS
instruments to manage open user facility. For detailed job description see
http://www.chem.nwu.edu/group_openings.htm. Applicants should send CV and
summary of relevant experience and qualifications to Dr. Robert N. Scott,
Department of Chemistry, Northwestern University, 2145 Sheridan Road,
Evanston, IL 60208-3113 or to r-scott-at-chem.nwu.edu. EEO/AA Employer.



From daemon Thu Feb 15 17:57:51 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 15 Feb 2001 18:36:51 -0500
Subject: RE: software for RDF of amorphous materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a paper in Applied Surface Science 167 (2000), pp. 59-68 entitled, "Structural studies of amorphous and crystallized tungsten nitride thin films by EFED, XRD, and TEM" by Y.G. Shen and Y.W. Mai that you should look up if you are interested in this topic.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Yan Xin [mailto:xin-at-magnet.fsu.edu]
Sent: Monday, February 12, 2001 12:32 PM
To: microscopy-at-sparc5.microscopy.com
Subject: software for RDF of amorphous materials


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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On-Line Help
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---------------------------------------------------------------
--------.


Dear all,

I am very interested in getting softwares to process electron
diffraction
patterns from disordered materials to obtain the G(r) (reduced
intensity
function), and J(r) (radial distribution function).

Any information on how to get these softwares is appreciated. In
particular, information on free ones are most welcome.

Regards
Yan Xin

=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================






From daemon Thu Feb 15 18:54:50 2001



From: Xinran Liu :      xinran.liu-at-UTSouthwestern.edu
Date: Thu, 15 Feb 2001 18:50:08 -0600
Subject: Confocal microscope selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are in the process to get a confocal microscope for a multi-user center.
The major application for this microscope will be using green fluorescent
proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal
pathways, including living cells.

Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any
suggestion and advice on the comparison of these two microscopes will be
highly appreciated.

Many thanks.

*******************************************
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
UT Southwestern Medical Center
6000 Harry Hines Blvd., NA4. 214A
Dallas, TX 75390-9111

Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu



From daemon Fri Feb 16 06:50:55 2001



From: Michelle.Taurino-at-aventis.com
Date: Fri, 16 Feb 2001 06:34:02 -0600
Subject: Confocal microscope selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


WE have the Zeiss LSM 510 and it's great. Their customer support/technical
support is outstanding.

Good Luck-
Michelle Taurino
Aventis Pharmaceuticals
Senior Scientist
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Xinran Liu [mailto:xinran.liu-at-UTSouthwestern.edu]
Sent: Thursday, February 15, 2001 7:50 PM
To: Microscopy-at-sparc5.microscopy.com


We are in the process to get a confocal microscope for a multi-user center.
The major application for this microscope will be using green fluorescent
proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal
pathways, including living cells.

Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any
suggestion and advice on the comparison of these two microscopes will be
highly appreciated.

Many thanks.

*******************************************
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
UT Southwestern Medical Center
6000 Harry Hines Blvd., NA4. 214A
Dallas, TX 75390-9111

Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu


From daemon Fri Feb 16 08:41:55 2001



From: Larry Nittler :      nittler-at-lepvax.gsfc.nasa.gov
Date: Fri, 16 Feb 2001 09:28:26 -0500
Subject: ultramicrotome recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many thanks to all who answered my request for ultramicrotome
information.

Cheers,
Larry

--
------------------------------------------------------
Larry R. Nittler, Interstellar Dust Buster

Laboratory for Extraterrestrial Physics, Code 691
NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/


From daemon Fri Feb 16 08:52:51 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 16 Feb 2001 09:54:54 -0500
Subject: Re: Confocal microscope selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 6:50 PM -0600 2/15/01, Xinran Liu wrote:
}
}
} We are in the process to get a confocal microscope for a multi-user center.
} The major application for this microscope will be using green fluorescent
} proteins and /or styrul dyes (e.g., FM 1-43) to label specific neuronal
} pathways, including living cells.
}
} Our consideration will be either "Leica TCS SP2" or "Zeiss LSM 510", any
} suggestion and advice on the comparison of these two microscopes will be
} highly appreciated.
}
} Many thanks.
}
**************************************

I cannot say anything about the Leica instrument, since I have never
used it. I can however, speak about the Zeiss LSM 510. I operate a
multi-user core facility and we have had an LSM 510 here since Nov.
1998. It has proven itself to be a reliable and fairly robust
instrument. We have aver 50 registered users. Some work quite
frequently, others rarely. Our regular users have all been trained
to use the system and work independently. The types of samples
examined also vary widely, from immunolabelled tissue sections
(paraffin and cryo) to live cell culutres expressing GFP. Overall,
we are very happy with it.

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Feb 16 11:06:28 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Fri, 16 Feb 2001 16:58:58 +0000 (GMT)
Subject: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had several requests for the asbestos protocol, so here it is.


1) Immerse sample in 10% HCL for 30 mins or until bubbles have stopped. (This removes filler material, usually calcite ICPMS 5-586.)

2) Decant and wash in water.

3) Evaporate till dry.

4) Grind to a powder using pestle and mortar. (Obviously, if the sample is suspected to be asbestos, take suitable precautions!)

5) Mount as a standard thick powder sample for theta-2theta diffractometry

6) Obtain data from 5-60 degrees (theta) using Cu K alpha radiation. (We have a fairly standard Philips diffractometer, with graphite monochromator and acceptor slit.)

In the UK, the notifiable asbestos compounds according to act of Parliament (don't ask me which one!) are:

Crocidolite ICPMS 29-1237
Amosite ICPMS 29-1170
Chrysotile ICPMS 31-808 or 25-645
Actinolite ICPMS 25-157
Anthophyllite ICPMS 9-455
Tremolite ICPMS 13-457

Other compounds which may be considered to be hazardous/asbestos-like are:
Quartz (SiO2) ICPMS 33-1161
Tridymite (SiO2) ICPMS 18-1170 or 14-260
Christobalite (SiO2) ICPMS 11-695
Kaolinite ICPMS 14-164
Amosite ICPMS 29-1170
Anorthite ICPMS 18-1202
Phlogopite ICPMS 10-492

You will need the ICPMS database and the ability to compare and/or search for matches.

Detection limit is about 5%, depending on the other materials in the sample.

This is not an accredited test. If you have a sample which doesn't show up positive, it may still contain asbestos - if in doubt, get an accredited test house to check.

No liability is accepted or implied in providing this information.



==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Fri Feb 16 12:43:52 2001



From: hagglund.kw-at-pg.com
Date: Fri, 16 Feb 2001 13:38:27 -0500
Subject: help me to find a book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been looking for a copy of "Food Microscopy", by Olga Flint. It is one
volume of the Royal Microscopy Society handbook series. I saw copies available
during the last MSA meeting, but cannot recall who the vendor was. Can somebody
point me towards a company or individual who may have a copy of this book
available for sale?

Karl Hagglund, Researcher
P&G Food and Beverage Analytical and Microbiology
6071 Center Hill Ave., Box 117
Cincinnati, OH 45224
(513)634-0146



From daemon Fri Feb 16 12:53:50 2001



From: hagglund.kw-at-pg.com
Date: Fri, 16 Feb 2001 13:50:04 -0500
Subject: finding a book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have been looking for a copy of "Food Microscopy", by Olga Flint. It is one
volume of the Royal Microscopy Society handbook series. I saw copies available
during the last MSA meeting, but cannot recall who the vendor was. Can somebody
point me towards a company or individual who may have a copy of this book
available for sale?

Karl Hagglund, Researcher
P&G Food and Beverage Analytical and Microbiology
6071 Center Hill Ave., Box 117
Cincinnati, OH 45224
(513)634-0146



From daemon Fri Feb 16 13:03:19 2001



From: Eric Steel :      eric.steel-at-nist.gov
Date: Fri, 16 Feb 2001 13:59:08 -0500
Subject: Re: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The US Environmental Protection Agency (US EPA) developed a series of
methods [Polarized Light Microscopy, Transmission Electron Microscopy,
Gravimetry (including acid dissolution and ashing), and X-ray Powder
Diffraction] for the analysis of asbestos in building materials that are
described in the following document:

EPA 600/R-93-116
Method for the Determination of Asbestos in Bulk Building Materials.
July 1993. (NTIS / PB93-218576)
[Updates and replaces Interim version in 40 CFR 763 Subpart F App A]


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-417-1321
100 Bureau Drive, Stop 8371
Gaithersburg, MD 20899-8371
http://www.nist.gov/cstl/div837/837.02/


From daemon Fri Feb 16 14:45:52 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 16 Feb 2001 15:39:49 -0500
Subject: Re: Confocal microscope selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We also have a multi-user facility and have two Leica systems, one an
inverted with multi-photon lasers and the other an upright with argon,
krypton, and HeNe lasers. When they work, they're great. However,
software has been less than desirable , as has service. Software keeps
being "upgraded" but not necessarily improved. I'm sure all the
instruments have problems but I think we've had more than our share with Leica.

At 06:50 PM 2/15/01 -0600, Xinran Liu wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-5700


From daemon Fri Feb 16 14:53:20 2001



From: hagglund.kw-at-pg.com
Date: Fri, 16 Feb 2001 15:41:38 -0500
Subject: Re: Finding a book... mission accomplished

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the help finding the book. I placed an order and (hopefully) have a
copy on the way.

Karl Hagglund, Researcher
P&G Food and Beverage Analytical and Microbiology
6071 Center Hill Ave., Box 117
Cincinnati, OH 45224
(513)634-0146



From daemon Fri Feb 16 15:11:03 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 16 Feb 2001 15:02:12 -0600
Subject: RE: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Any comments on this one?

I have (not) identified asbestos... That is, some insulating material was
brought to me with the question, "Is it asbestos?". I was able to say that
it was not since careful EDS examination of the powdered material revealed
no trace of the elements associated with asbestos. It was 100% gypsum.

Even if not certified, this would seem to produce a satisfactory answer.
..Or are the regulations now so absurd that officialdom is concerned
someone may mistake brass for asbestos?
;)

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

http://home.att.net/~woody.white


From daemon Fri Feb 16 15:34:10 2001



From: Cindy Kleier :      j-kleier-at-northwestern.edu
Date: Fri, 16 Feb 2001 15:27:37 -0800
Subject: PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Need assistance on Gatan PIPS. Believe DP and MDP are functioning
properly, but specimen chamber vacuum is "glitchy."

Symptoms: DP light on constant, MDP light never comes on, DP test reads
19.9 at all times, Lights on vac and vent do not glow nor will sample raise
or lower.

Please respond off list.








J. Cindy Kleier
Specimen Preparation Engineer
Northwestern University
Evanston, IL. USA
847-491-7807
j-kleier-at-northwestern.edu



From daemon Fri Feb 16 15:41:32 2001



From: Cindy Kleier :      j-kleier-at-northwestern.edu
Date: Fri, 16 Feb 2001 15:35:17 -0800
Subject: Low Speed Saw

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, does anyone have an old or extra low speed saw that they don't use
anymore? Please respond off list.

Thank you,

Cindy Kleier








J. Cindy Kleier
Specimen Preparation Engineer
Northwestern University
Evanston, IL. USA
847-491-7807
j-kleier-at-northwestern.edu



From daemon Fri Feb 16 16:31:30 2001



From: Joseph Passero :      jp-at-spacelab.net
Date: Fri, 16 Feb 2001 17:26:22 -0500
Subject: (LM) Wanted Wild M20 Part

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am looking for a stage (a standard stage for transmitted light) for a Wild M20.

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net


From daemon Fri Feb 16 16:52:06 2001



From: COURYHOUSE-at-aol.com
Date: Fri, 16 Feb 2001 17:48:07 EST
Subject: Re: help me to find a book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


fatbrain.com and barnes and noble and amazon.com all list this book for 20
something bucks.
hope this helps...

ed sharpe archivist for SMECC

{ { Subj: help me to find a book
Date: 2/16/01 2:06:41 PM US Mountain Standard Time
From: hagglund.kw-at-pg.com-at-sparc5.microscopy.com
To: microscopy-at-microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


I have been looking for a copy of "Food Microscopy", by Olga Flint. It is
one
volume of the Royal Microscopy Society handbook series. I saw copies
available
during the last MSA meeting, but cannot recall who the vendor was. Can
somebody
point me towards a company or individual who may have a copy of this book
available for sale?

Karl Hagglund, Researcher
P&G Food and Beverage Analytical and Microbiology
6071 Center Hill Ave., Box 117
Cincinnati, OH 45224
(513)634-0146




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From daemon Fri Feb 16 17:00:47 2001



From: Jim Howe :      jh9s-at-virginia.edu
Date: Fri, 16 Feb 2001 17:55:24 -0500
Subject: New TEM/XRD Textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague:

We are e-mailing to let you know that our textbook:

"Transmission Electron Microscopy and Diffractometry of Materials"

has just been published by Springer. This textbook and its earlier drafts
have been used for over a decade in courses on transmission electron
microscopy (TEM) and x-ray diffractometry (XRD) at the California Institute
of Technology and University of Virginia. These courses are taken by
graduate students and advanced undergraduates in materials science,
solid-state physics, and solid-state chemistry.

The book emphasizes themes common to both diffraction and microscopy, such
as wave coherence, scattering from atoms, and the formation and analysis of
diffraction patterns. It also describes the uniqueness of TEM and XRD.
The book has 748 pages through the index, includes approximately 500
accompanying illustrations, and a total of 151 problems at the ends of
chapters. The Appendix provides a set of introductory TEM laboratory
exercises, and contains up-to-date reference data for both TEM and XRD.
The chapters are:

1. Diffraction and the X-Ray Powder Diffractometer, p. 1
2. The TEM and its Optics, p. 63
3. Scattering, p. 123
4. Inelastic Electron Scattering and Spectroscopy, p. 167
5. Diffraction from Crystals, p. 225
6. Electron Diffraction and Crystallography, p. 275
7. Diffraction Contrast in TEM Images, p. 339
8. Diffraction Lineshapes, p. 423
9. Patterson Functions and Diffuse Scattering, p. 467
10. High-Resolution TEM Imaging, p. 523
11. Dynamical Theory, p. 595
12. Bibliography, p. 661
13. Appendix, p. 675
14. Index, p. 735

To help with teaching, sections containing specialized or higher-level
material are marked, and paths around these sections are suggested.
A secure web site has been established with worked solutions to 3/4 of
the problems in the text.

A web site with excerpts from many chapters is:

http://www.caltech.edu/~matsci/btf/TEM_Book.html

Publication and order information can be found at the Springer site:

http://www.springer.de/cgi-bin/search_book.pl?isbn=3-540-67841-7

We hope that you find our book useful and look forward to hearing from you
about it.

Sincerely yours,
Brent Fultz and Jim Howe

Brent Fultz, Professor of Materials Science
California Institute of Technology, mail 138-78
Pasadena, CA 91125, USA
Office: 626-395-2170; Fax: 626-795-6132; E-mail: btf-at-hyperfine.caltech.edu

James M. Howe, Professor and Director of the Electron Microscope Facility
Department of Materials Science & Engineering
University of Virginia
Charlottesville, VA 22904-4745, USA
Office: 804-982-5646; Fax: 804-982-5660; E-mail: jh9s-at-virginia.edu

























From daemon Fri Feb 16 17:50:58 2001



From: Lewis McCrigler :      lmm7001-at-Humboldt.edu
Date: Fri, 16 Feb 2001 15:49:15 -0800
Subject: Wild M5 Eyepieces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We are looking for some Wild 20x oculars to equip a Wild M5 stereo
microscope. If anyone has any extras or knows of a source, please respond
directly to:
LMM7001-at-humboldt.edu

Thanks,
Lewis mcCrigler
Equipment Tech.
Humboldt State University



From daemon Sat Feb 17 17:02:28 2001



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Sat, 17 Feb 2001 17:47:36 -0500
Subject: RE: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Woody,

I think this is fundamentally an EDS detection limit issue. Question is when
you see Ca, S, and possibly O peaks only on a spectrum from your insulation,
how sure you can be to say that you don't have 1 vol% of Si-Mg-Fe-Mn etc
while the 1 vol% is the cut-off concentration in the US regulation governing
asbestos analysis of building materials. Personally, I wouldn't be very
comfortable with an EDS un-identification of asbestos.

-cy, Ex-expert of asbestos analysis



-----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-mcdermott.com]
Sent: Friday, February 16, 2001 4:02 PM
To: 'Microscopy-at-MSA.Microscopy.Com'



Any comments on this one?

I have (not) identified asbestos... That is, some insulating material was
brought to me with the question, "Is it asbestos?". I was able to say that
it was not since careful EDS examination of the powdered material revealed
no trace of the elements associated with asbestos. It was 100% gypsum.

Even if not certified, this would seem to produce a satisfactory answer.
.Or are the regulations now so absurd that officialdom is concerned
someone may mistake brass for asbestos?
;)

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

http://home.att.net/~woody.white


From daemon Sat Feb 17 17:11:49 2001



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Sat, 17 Feb 2001 18:05:59 -0500
Subject: RE: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Forgot to mention that my previous comments only apply to EDS/SEM that
appears to be what Mr. Woody meant. In the case of TEM analysis, Woody
should be 100% right if the beam is focused on each individual fibers and
sufficient number of fibers are checked.

-cy, Ex-fiber counter on TEM, PLM and PCM


-----Original Message-----
} From: Ni, Chao-Ying
Sent: Saturday, February 17, 2001 5:48 PM
To: 'White, Woody N.'; 'Microscopy-at-MSA.Microscopy.Com'



Any comments on this one?

I have (not) identified asbestos... That is, some insulating material was
brought to me with the question, "Is it asbestos?". I was able to say that
it was not since careful EDS examination of the powdered material revealed
no trace of the elements associated with asbestos. It was 100% gypsum.

Even if not certified, this would seem to produce a satisfactory answer.
.Or are the regulations now so absurd that officialdom is concerned
someone may mistake brass for asbestos?
;)

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

http://home.att.net/~woody.white


From daemon Sat Feb 17 19:05:16 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Sun, 18 Feb 2001 11:50:55 +1100
Subject: Re: Confocal microscope selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We recently had a Leica TCS SP2 installed, and it has been running just
fine, software is easy to use and service has been great. In a previous
institution we had two Leica TCS's - hardware fine, software OK, service
also great.

IMHO, the main considerations are first, service and reliability - and
service varies quite a bit with location for each company. Second, do you
really need the SP function of the Leica? If not, I think these two
instruments are much of a muchness for routine work. There are pluses and
minuses on each side and you need to decide what your priorities are.
Third, what kind of price/package can you negotiate?

Another 2¢ worth.....
cheers,

At 06:50 PM 2/15/01 -0600, Xinran Liu wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Sun Feb 18 07:28:37 2001



From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Sun, 18 Feb 2001 08:21:28 -0500 (EST)
Subject: Immonogold-antigen retrieval

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I need to do some immunogold TEM with antibodies that work well
for light microscopy immunohistochemistry but require various antigen
retrieval treatments. I used to do a lot of immunogold in the old
pre-antigen retrieval times. I wonder what are the current developments in
this area. Are antigen retrieval techniques used on LR White sections?
Thanks for any suggestions,

Sarka Lhotak, PhD.

Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada
lhotaks-at-mcmaster.ca


From daemon Sun Feb 18 11:53:19 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Sun, 18 Feb 2001 11:41:09 -0600
Subject: RE: Asbestos protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I understand the situation. In addition to "bulk" analysis, numerous
locations were examined using higher and higher BSE magnifications to sort
out any (non existent in this case) Z differences. EDS was used for each as
well. In addition, with the appropriate choice of accelerating potential
and count integral, my EDS could easily detect { 0.25%. Granted, this is
not a very efficient method and if ANY of the questionable elements were
detected, nothing is proved.

Woody

} Dear Woody,
}
} I think this is fundamentally an EDS detection limit issue.
} Question is when
} you see Ca, S, and possibly O peaks only on a spectrum from
} your insulation,
} how sure you can be to say that you don't have 1 vol% of
} Si-Mg-Fe-Mn etc
} while the 1 vol% is the cut-off concentration in the US
} regulation governing
} asbestos analysis of building materials. Personally, I
} wouldn't be very
} comfortable with an EDS un-identification of asbestos.
}
} -cy, Ex-expert of asbestos analysis
}
}
}
} -----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-mcdermott.com]
} Sent: Friday, February 16, 2001 4:02 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Asbestos protocol
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} Any comments on this one?
}
} I have (not) identified asbestos... That is, some insulating
} material was
} brought to me with the question, "Is it asbestos?". I was
} able to say that
} it was not since careful EDS examination of the powdered
} material revealed
} no trace of the elements associated with asbestos. It was
} 100% gypsum.
}
} Even if not certified, this would seem to produce a
} satisfactory answer.
} ..Or are the regulations now so absurd that officialdom is concerned
} someone may mistake brass for asbestos?
} ;)
}
} Woody White
} McDermott Technology, Inc.
} http://www.mtiresearch.com/
}
} http://home.att.net/~woody.white
}


From daemon Mon Feb 19 07:13:56 2001



From: MSimko-at-uss.com
Date: Mon, 19 Feb 2001 07:34:30 -0500
Subject: Polaroid DMC camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to thank everyone who sent suggestions for cleaning the dust
from a Polaroid DMC digital camera. We had great luck using a lint-free
cloth moistened with methanol and very gently wiping the filter surface
after opening the shutter. Practically all of the "spots" we were seeing
on our digital images were successfully removed and the camera is now
capable of meeting our most demanding needs. Polariod really came through
with excellent technical support via the Listserver. If anyone is
interested in learning more details (I know some of you mentioned you had
the same "problem"), please feel free to contact me and thanks again for
all your help.

Michael Simko
Research Manager Metallography Group
U. S. Steel Research and Technology Center




From daemon Mon Feb 19 07:13:57 2001



From: MSimko-at-uss.com
Date: Thu, 15 Feb 2001 08:06:23 -0500
Subject: Polaroid DMC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wanted to thank everyone who sent suggestions for cleaning the dust from
a Polaroid DMC digital camera. We had great luck using a lint-free cloth
moistened with methanol and very gently wiping the filter surface after
opening the shutter. Practically all of the "spots" we were seeing on our
digital images were successfully removed and the camera is now capable of
meeting our most demanding needs. Polariod really came through with
excellent technical support via the Listerserver. If anyone is interested
in learning more details (I know some of you mentioned you had the same
"problem"), please feel free to contact me and thanks again for all your
help.

Michael Simko
Research Manager Metallography Group
U. S. Steel Research and Technology Center



From daemon Mon Feb 19 07:13:57 2001



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Mon, 19 Feb 2001 13:09:10 -0000
Subject: Janus green mitochondrial stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues


I am trying to find some information on staining mitochondria with Janus
green, including the staining mechanism. However nothing relevant seems to
be available in the internet or in my technical books.
This is mainly for teaching purposes.

Can anyone send me information on the subject and/or relevant URL(s) to
consult ?

Thanks for your help
Dr. A.P. Alves de Matos
apmatos-at-ip.pt



From daemon Mon Feb 19 12:14:10 2001



From: Luvstodance99-at-aol.com
Date: Mon, 19 Feb 2001 13:07:37 EST
Subject: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are searching for companies that provide cleaning and maintenance of
microscopes.

Rhonda Blassingill
Harrisonburg Cytology


From daemon Mon Feb 19 12:53:27 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Mon, 19 Feb 2001 13:48:53 -0500
Subject: Food Structure and Functionality Symposium 2001 - Feb 19th

Contents Retrieved from Microscopy Listserver Archives
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Food Structure & Functionality Symposium 2001
An international symposium leading food structure & Functionality studies through the 21st century

"webaddress at the AOCS site http://www.aocs.org/member/division/fsff/index.htm"

Being held at the 92nd AOCS Annual Meeting & Expo, May 13_16, 2001, Minneapolis
Convention Center, Minneapolis, Minnesota, USA. For information, e_mail us at
meetings-at-aocs.org

The symposium has two themes:
* New and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure_function relationships in foods;
* Food system studies covering any part of the processing chain _ from the raw material to the final product, including trouble shooting.
---------------------------------------------------
Tentative Program (confirmed as of February 19th, 2001)

May 13th _ Short Courses (short courses will run for a full day, and will run concurrently)

Short Course #1 Food Contaminants.
Course organiser: Mark Auty (mauty-at-moorepark.teagasc.ie)

Short Course #2
Understanding structure-function relationships in food systems through specific localisation methods and microscopy.

For more information contact Marcel Paques (paques-at-nizo.nl)
---------------------------------------------------

May 14th-16th inclusive -Technical sessions (6 sessions will run over 3 days)

Monday, May 14th, 2001
Morning
Symposium opening Plenary lecture _ Food Quality and why the Structure matters
P. Lillford, Unilever Research, Colworth House UK

Session 1: Dairy Products and Fat Based Foods Session _ chairs M. Auty and M. Paques

Milk protein polysaccharide interactions in aqueous solutions and oil-in-water emulsions.
H. Singh*, Y. Hemar & P. Munro, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand

Structural functions of dairy ingredients in products formulated with taro flour.
C. Onwulata USDA/ARS,Eastern Regional Research Center,Wyndmoor, PA 19038

Confocal imaging of galactomannan mode of action in recrystallisation of ice in model ice cream
D. Ferdinando, Unilever Research Colworth House, UK

Heating of Food Proteins in a Closed System at High Temperature
N. Kitabatake, Kyoto University, Japan

Milk Protein -molecular components and functional properties
N.C. Ganguli ,Indian Dairy Association

Afternoon
Session 2: Food Safety _ chairs J. W. Arnold and R. Droleskey
Prevention of Foodborne Illness Through Sanitation and Processing Technology
J.W. Arnold; USDA/ARS, Russell Research Center; Athens, GA 30604

PreHarvest Intervention Strategies to Control Foodborne Pathogens in Poultry
J. A. Byrd; USDA/ARS/SPARC; College Station, TX 77845

Interactions of Competitive Exclusion Cultures with the Intestinal Mucosa of Newly Hatched Chicks
R. Droleskey; USDA/ARS/SPARC; College Station, TX 77845

Adhesion of Salmonella on Alfalfa Sprouts
A. Chartowski; USDA/ARS, Western Regional Research Center; Albany, CA 94710

Growth of Fusarium moniliforme Dependent upon Corn Tissue Type
I. E. Yates; USDA/ARS, Russell Research Center; Athens, GA 30604

Dedicated poster viewing 4:00_6:00PM

Evening: Round Table Discussion _ topic to be announced

---------------------------------------------------
Tuesday, May 15th, 2001
Morning
Session 3: New Methods and Techniques for Food Structure and Functionality Analysis _chair
K.Groves
Applications of Scanning Near Field Optical Microscopy in the analysis of food and food-related
materials.
A.R. Kirby*, P. Gunning, P.J. Wilde and V. J. Morris, Institute of Food Research,
Norwich, England

Diffusing wave spectroscopy - a new and non-invasive method for the investigation of the structure, dynamics and interactions in complex food systems
M. Alexander* and P. Schurtenberger, Universite de Fribourg, Switzerland

AFM as a tool for characterising polysaccharides and their modification.
V.J. Morris*, A.P. Gunning, A.R. Round, E. Adams, E. Kroon and G. Williamson, Institute of Food Research, Norwich, England

Micro-rheology - functional properties of food systems and their origin in microstructure
M. Paques, Y. Nicolas, G. van Aken, H. Tromp, A. Janssen, Unilever Research
Vlaardingen, The Netherlands

Immunolocalization of Transgenic Protein in Wheat Endosperm
M. L. Parker (invited),* E. Stoger, *R. Casey and *P. Christou, Institute of Food
Research, Norwich, England and *John Innes Centre, Norwich, England

Food: How complex can it be?
E. Esselink ,Unilever Research Vlaardingen, The Netherlands

Multiple Extrusion Module for determining changes in consistency during working - a new method.
J.F.C. Van Maanen*, B. Dunnewind, and A. Jurgens. TNO Nutrition and Food Research
Institute, The Netherlands

Specific detection of components and compounds through microscopical immunodetection
J. Leunissen, AURION: Immuno-Gold Reagents & Accessories, The Netherlands

Food Structure and Functionality Division Luncheon. Dr. Brian Brooker (Institute of Food Research, England, retired), the recipient of the Food Structure and Functionality Division Award, will give a
presentation entitled: Fat crystals - the importance of being small.

Afternoon
Session 4: Agricultural Applications of Microscopy and Imaging Session chairs D.F.Wood and P. Allan-Wojtas

The Utility of Sorting in Agriculture.
H. J. Arnott, Department of Biology, University of Texas at Arlington, Texas

The Potential for Automatic X-ray Sorting of Insect Infested Grain
R. Haff . USDA - ARS -WRRC, Albany, California.

Automated Sorting of Almonds with Embedded Shell by Laser Transmittance Imaging
T. Pearson* R. Young, USDA -ARS- WRRC, Albany, California.

Use of a GFP-transformed strain of Fusarium graminearum to study ear rot development in corn
S. S. Miller. , AAFC-ECORC, Ottawa, Ontario, Canada.

Popping modifies endosperm structure and improves digestibility in maize and sorghum.
M.L. Parker, Institute of Food Research, Norwich, England.

Microstructural Changes in Rice During Cooking
D. Wood* and P.C. Yu . USDA -ARS- WRRC, Albany, California.

The Food Structure and Functionality Division Member meeting will be held immediately following this session. All are welcome.

--------------------------------------------------- Wednesday, May 16th, 2001
Morning
Session 5: Ingredients and Food Processing chairs D. Kittleson and J. Charbonneau

Water continuous fat crystal networks in ice cream induced by unsaturated monoglycerides
N. M. Barfod , Danisco Cultor, Brabrand, Denmark

High pressure application of food systems and its impact on functional ingredients
B. Tauscher*, P. Butz,and A.F. Garcia, Federal Research Center for Nutrition, Karlsruhe, Germany

Specificity and application of lipolytic enzymes in bread making processes
T.B. Frandsen, T. Spendler, G. Budolfsen, L. Christiansen and J. B. Neilsen, Novozymes A/S, Bagsvaerd, Denmark

The development of bubble structure in bread doughs.
M.B. Whitworth and J.M. Alava, Campden & Chorleywood Food Research Association,
UK

Structure-texture relationships in heat resistant chocolate
K. Groves, P. Subramaniam and O. Murphy, Leatherhead Food Research Association, UK

Reduction of oil uptake by methyl-cellulose coatings applied to fried dough
M. A. Garcia, C. Ferrero, N. Bertola, M. Martino* and N. Zaritzky, CIDCA CONICET. Fac. de Ciencias Exactas and Fac. de Ingenieria, Universidad Nacional de La Plata, Argentina.

Afternoon
Session 6: Colloidal and Interfacial Sciences _ chairs D. Pechak and M. Paques

Rheology and Structure of Particulate Protein Gels
T. van Vliet, Wageningen Centre for Food Sciences,Wageningen, The Netherlands

Time-temperature studies of food polymer gelation.
Paulson, AT., Nickerson, M.T. and Speers, R.A. Department of Food Science and TechnologyDalhousie University, Halifax, NS, Canada

---------------------------------------------------
Contact information for the chairs is shown below, in alphabetical order:

Paula Allan-Wojtas
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre,
Kentville, Nova Scotia, Canada
B4N 1J5
Tel: (902) 679-5566
FAX: (902) 679-2311
email: allanwojtasp-at-em.agr.ca

Judy Arnold
USDA -ARS -RRC
950 College Station Rd.
P.O. Box 5677
Athens, GA 30604-5677
USA
Tel: (706) 546-3515
FAX: (706) 546-3068
email: jarnold-at-ars.usda.gov

Mark Auty
Dairy Products Research Centre
TEAGASC
Moorepark, Femoy, Co. Cork
Ireland
Tel: 011-353-25-42447
FAX: 011-353-25-42340
email: mauty-at-moorepark.teagasc.ie

James E. Charbonneau
National Food Processors Association
Food Chemistry and Packaging Department
1401 New York Ave, NW
Washington, D.C. 20005
USA
Tel: (202) 639-5972
FAX: (202) 639-5991
email: jcharbo-at-nfpa-food.org

Kathy Groves
Leatherhead Food Research Association
Randalls Road, Leatherhead
Surrey KT22 7RY
England
Tel: 44 0132 822330
FAX: 44 0132 386228
email: kgroves-at-lfra.co.uk

Diana Kittleson
Pillsbury TPC Labs
737 Pelham Blvd.
St. Paul, MN 55114
USA
Tel: (651) 917-5859
FAX: (651) 917-5850
email: dkittleson-at-pillsbury.com

Tony McKenna
New Zealand Dairy Institute
Private Bag 11 029
Palmerston North,
New Zealand
Tel: 011 64 6 350 4649
FAX: 011 64 6 356 1476
email: tony.mckenna-at-nzdri.org.nz

Marcel Paques
Wageningen Centre for Food Sciences/Unilever Research Vlaardingen
P.O. Box 20, 6710 BA Ede
The Netherlands
Tel: 011 31 318 659690
FAX: 011 31 318 650400
email: paques-at-nizo.nl

David Pechak
Kraft Technology Centre
801 Waukegan Road
Glenview, IL 60025
USA
Tel: (847) 646-4808
FAX: (847) 646-3864
email: dpechak-at-kraft.com

Delilah Wood
USDA -ARS -WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5653
FAX: (510) 559-5777
email: wood-at-pw.usda.gov



From daemon Mon Feb 19 13:00:16 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Mon, 19 Feb 2001 13:55:45 -0500
Subject: Food Structure and Functionality Symposium 2001 - Short

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Food Structure & Functionality Symposium 2001
An international symposium leading food structure & Functionality studies through the 21st century

"webaddress at the AOCS site http://www.aocs.org/member/division/fsff/index.htm"

Being held at the 92nd AOCS Annual Meeting & Expo, May 13_16, 2001, Minneapolis
Convention Center, Minneapolis, Minnesota, USA. For information, e_mail us at
meetings-at-aocs.org

May 13th - Short Courses (short courses will run for a full day, and will run concurrently)

Short Course #1 Food Contaminants.
Most food manufacturing companies possess light microscopes, but seldom use them to their full
potential. Microscopy is a powerful problem-solving tool for both contaminant analysis and food
ingredient characterisation. This short course is aimed at Quality Control/Assurance and Food
Product R&D personnel. The course comprises lectures and hands-on tuition from expert
microscopists. The aim of the course is to provide delegates with a practical introduction to food
microscopy, in particular contaminant (foreign body) identification and food ingredient
characterisation.

Programme topics include:
Getting the most from your light microscope - basic setting up and contrast techniques
Preparing food samples for microscopy
How to approach foreign body identification
Identifying plant and animal remains
Identifying glass, plastics, minerals
Instrumental methods for contaminant analysis
Microscopy in product research and development

Course contributors:
John Shane (McCrone Research Institute, USA)
Jim Charbonneau (National Food Processors Association, USA)
Kathy Groves (Leatherhead Food Research Association, UK).
Diana Kittleson (Pillsbury Technical Labs, USA)
Mark Auty (Teagasc, Moorepark, Ireland)
Course organiser: Mark Auty
(mauty-at-moorepark.teagasc.ie)

Short Course #2
Understanding structure-function relationships in food systems through specific
localisation methods and microscopy.

Spreadability, shelf life, fracture behaviour, creaminess, and mouthfeel are examples of functional properties of food products. These properties originate from the microscopic structure of the products. Specific localisation techniques and microscopy are powerful new tools to facilitate intelligent modification of ingredient composition or processing to obtain targeted product properties. The short course is aimed at R&D personnel in the Foods area (fundamental research, innovation, and product development). The course provides lectures and an intensive hands-on practical part enabling participants to gain sufficient basic knowledge and skills to set-up and implement the methods in their own work.

Programme topics include:
D Introduction on specific localisation methods and principles
D Localisation strategies, marking options, and imaging approaches
D Experimental set-up, preparation and incubation procedures
D Demonstration cases
D Hands-on practical sessions

Course contributors:
Jan Leunissen (Aurion: immuno Gold Reagents & accessories, custom labeling, The Netherlands)
Gary Fulcher (University of Minnesota, USA) Paula Allan-Wojtas (Agriculture and Agri-Food,
Canada).
Marcel Paques (Wageningen Centre for Food Sciences/Unilever Research Vlaardingen, The
Netherlands)

For more information contact Marcel Paques (paques-at-nizo.nl)
---------------------------------------------------

Technical sessions (6 sessions will run over 3 days) will follow the short courses from May 14th-16th inclusive.

---------------------------------------------------

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Mon Feb 19 14:32:53 2001



From: RCHIOVETTI-at-aol.com
Date: Mon, 19 Feb 2001 15:26:30 EST
Subject: Re: Wild M5 Eyepieces

Contents Retrieved from Microscopy Listserver Archives
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Lewis,

I'm not sure about this, but I recall someone telling me that the current
Leica eyepieces for the MZ series of stereomicroscopes will also work on the
older Wild M5, M8, etc.

It would be worth a shot to call your local Leica rep, or contact Leica
Customer Service at 1-800-248-0123.

Good luck!

Bob Chiovetti
GTI Microsystems


From daemon Mon Feb 19 16:36:51 2001



From: Hunter O'Reilly :      hunter-at-ArtByHunter.com
Date: Mon, 19 Feb 2001 15:36:41 -0600
Subject: Microscopic Images for Art/Science Exhibit

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Dear Scientists,

My name is Hunter O'Reilly. I recently received my Ph.D. in genetics from
the University of Wisconsin-Madison in December 2000. I am also a
contemporary artist currently working full-time on an art project called
Radioactive Biohazard. Radioactive Biohazard is a project that integrates
art and science to bring awareness of issues in biotechnology to the public
in a positive light. A free catalog containing scientifically accurate yet
simple explanations of the artworks will be available to the public.

I am very interested in using electron micrograph images of viruses (i.e.
HIV, hepatitis, rabies, polio, herpes, ebola, adenovirus) in digital
artworks for the second part of this project described below. I am also
interested in other visually interesting microscopic images. Any artwork
that used any part of a scientist's image would give the scientist credit
for that part of the image both in the exhibit and publication of the
artwork. Donations of images can also be made anonymously. I have high
speed internet access. Very large files can be sent to me via email.

My artwork has appeared on the covers of several international scientific
journals including Nature Reviews Genetics, Medical Genetics, The EMBO
Journal, Promega's Neural Notes, and Developmental Dynamics. You can
view some of these covers at
http://www.artbyhunter.com/media/

Please email questions and/or microscopic images to Hunter O'Reilly at

hunter-at-artbyhunter.com

You can view my artwork on my website at
http://www.ArtByHunter.com

Thank you for your time and consideration! Please read further for more
information.

I have received donations of images from Biotechniques and the following
individuals for use in this project.

Biotechniques: A Journal of Laboratory Technology for Bioresearch and Eaton
Publishing Co.
Dr. Peter Angeletti, Post-Doc at the McArdle Laboratory for Cancer Research
Dr. James Briscoe, Columbia University
Dr. James Ellingboe, Scientific Editor of Biotechniques
Dr. Prakash Hande, Columbia University
Dr. Mary McCarthy, Associate Scientific Editor of Biotechniques
Dr. James A. Priess, Fred Hutchinson Cancer Research Center
Dr. Alison Roberts, University of Rhode Island
Dr. Charlotte Schubert, formerly at the Fred Hutchinson Cancer Research
Center


Radioactive Biohazard will premiere at the Walker's Point Center for the
Arts in Milwaukee, Wisconsin April 20, 2001.

The exhibit will consist of three parts:
1. oil paintings reinterpreting science as art exploring themes such as
cloning and genetic identity--For example I am creating one oil painting on
the theme of stem-cell research, and the potential to create organs for
transplants from stem-cells. I am creating another oil painting regarding
the use of human cloning as a treatment for infertility. The explanations
in the catalog will be simple for the general public, but scientifically
accurate. Controversial issues surrounding this research will also be
discussed. I will write the original explanations, and have other
scientists proofread and make suggestions before they are finalized.
2. enlargements of micrographs of cells, viruses and bacteria highlighted
with neon tubing--These artworks will be digital collages of microscopic
images. With some of the collages, I will create a landscape. With others
I may superimpose the image of a person. In the catalog and next to each
artwork, there will be a diagram siting the source of each part of the
image, explaining what the image is, and possibly how the image is
obtained. The creation of the digital collage will be artistic, but there
will be scientific explanations to accompany the works. These artworks
will allow the general public to see images they have never seen before.
3. a laboratory bench installation: an artistic interpretation of a
laboratory bench, equipment etc.

You will find a longer description at
http://www.artbyhunter.com/artgallery/radbio.html

My homepage is at
http://www.artbyhunter.com

Thank you for your time and consideration! I look forward to hearing from
you!

Sincerely,

Hunter O'Reilly, Ph.D.


From daemon Mon Feb 19 17:41:58 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Tue, 20 Feb 2001 10:36:26 +1000
Subject: Re: Immonogold-antigen retrieval

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Hi,

It's a couple of years since I did any antigen retrieval and then I
did it on tissue pieces, which were labelled preembedding. I found
citrate buffer heated to about 95deg was effective and the most
gentle. Have no info on LR White sections, except that in my hands
they never seem to label with anything!

Diana

} ------------------------------------------------------------------------
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--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Mon Feb 19 17:56:11 2001



From: Wizardofthelab-at-aol.com
Date: Mon, 19 Feb 2001 18:52:42 EST
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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Look in the phone book.

Why must we endure these mundane questions, can anybody in this world think
for themselves.


From daemon Mon Feb 19 20:57:54 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 19 Feb 2001 20:52:33 -0600
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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} From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com}
} Look in the phone book.
}
} Why must we endure these mundane questions, can anybody in this world
think
} for themselves.

Not all of us live in a city, state or even a country that has a
microscope repair and service company.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger





From daemon Mon Feb 19 20:57:54 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Tue, 20 Feb 2001 08:09:24 +0200
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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In some areas it may not be as simple as looking in the phone book.
This listserver was meant to answer question not as a "put down' media.

Thank You,

Earl
----- Original Message -----
} From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, February 19, 2001 3:52 PM


The question may have been unnecessary, but wasn't this
response rather abrupt, unhelpful and unfriendly, particularly as it
was anonymous?

} Look in the phone book.
}
} Why must we endure these mundane questions, can anybody in this world
} think for themselves.

Have a good day.




Robin H Cross (Mr)
Director : Electron Microscopy Unit
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
email: r.cross-at-ru.ac.za
http://www.ru.ac.za/emu/index.htm

** remember ICEM-15 in Durban in September 2002 **


From daemon Tue Feb 20 01:45:31 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 20 Feb 2001 20:46:14 GMT+1200
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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}
} Look in the phone book.
}
} Why must we endure these mundane questions, can anybody in this
} world think for themselves.
}


Very rude.

And coming from someone sheltering behind anonymity

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Feb 20 02:34:14 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 20 Feb 2001 19:28:53 +1100
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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Hi,

A couple of hints. Here, a local microscope maintenance company (not
listed in the phone book except in yellow pages under scientific
instruments) comes around once a year and is known by several other company
reps. Your microscope sellers may know of someone, as may other
microscopy-related suppliers, even if you can't find anything listed in the
phone book or on the web. The suppliers are usually unwilling to do this
type of maintenance themselves, but there are local exceptions, so it's
worth asking.

good luck,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Feb 20 04:31:00 2001



From: Chris Holp :      holpcr-at-earthlink.net
Date: Tue, 20 Feb 2001 04:25:00 -0600
Subject: Failure analysis of plastics and ceramics

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I would like to know if anyone can recommend any books regarding the failure
modes and mechanisms of plastics and ceramics. My searches have been yielded
little (read as nothing), and my library is woefully lacking.

Thanks to all in advance for your help!

Chris Holp
ATC Materials Lab
holpcr-at-earthlink.net




From daemon Tue Feb 20 06:26:22 2001



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 20 Feb 2001 07:22:18 -0500 (EST)
Subject: anonymity and microscope cleaning

Contents Retrieved from Microscopy Listserver Archives
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Several people have already beat me to the punch, as they say, but I
thoroughly agree with the rudeness of anonymity, even if it is
unintentional. I have corresponded off-line with Nestor on this and my
recollection is that the rules of the list preclude anonymous postings. Any
one of us can occasionally make this error. But a small but annoying
percentage of the members still post anonymously every time.

So why must I endure postings on asbestos when my interest is
cathodoluminescence? Because it is part of the purpose of the list. And why
must I endure questions from novices? Because this has also grown to be a
purpose of the list and one that has been rather well served. And frankly it
occasionally empowers me to ask questions that may seem stupid to those who
know all things.





} Look in the phone book.
}
} Why must we endure these mundane questions, can anybody in this
} world think for themselves.
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)


From daemon Tue Feb 20 06:38:51 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 20 Feb 2001 06:30:40 -0600
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
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At 08:52 PM 2/19/01 -0600, Gordon Couger wrote:
} } From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com}
} } Look in the phone book.
} }
} } Why must we endure these mundane questions, can anybody in this
} } world think for themselves.
}
} Not all of us live in a city, state or even a country that has a
} microscope repair and service company.

Speak with respect and a humble aspect, for he is
WizardOfTheLab ... -at-aol.com ! :-)

- John



From daemon Tue Feb 20 07:53:25 2001



From: James F. Sanzo :      jfs-at-seas.upenn.edu
Date: Tue, 20 Feb 2001 08:43:21 -0500
Subject: Re: Cleaning and maintenance of microscopes

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Dear "wizard",

Although you were afraid to use your real name, and instead used an absurd
pseudonym, allow me to enlighten you just the same. The asker of the
question you have objected to was looking for a RECOMMENDATION.
Facilitating the sharing of facts and opinions in a professional, helpful
way is what this listserver does so well.

Perhaps you should consider unsubscribing?

Thanks,
Jim

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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
James F. Sanzo, Ph.D
Dept. of Bioengineering
120 Hayden Hall
3320 Smith Walk
University of Pennsylvania
Philadelphia, PA 19104-6392

Phone: 215-573-5191
Fax: 215-573-2071

http://www.seas.upenn.edu/be/labs/confocal/



From daemon Tue Feb 20 09:45:03 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 20 Feb 2001 09:39:56 -0600
Subject: RE: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a question to Nestor:
May be no more anonymous subscribers?

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Monday, February 19, 2001 5:53 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Cleaning and maintenance of microscopes
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Look in the phone book.
}
} Why must we endure these mundane questions, can anybody in
} this world think
} for themselves.
}


From daemon Tue Feb 20 10:29:20 2001



From: Wizardofthelab-at-aol.com
Date: Tue, 20 Feb 2001 11:25:34 EST
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am sorry you all are taking this so terrible, I was not intending to be
rude. I was merely pointing out the fact that every time someone has a slight
issue they want to magnify it in this forum. I think this forum can be used
for better issues than this.

It seems to me that we are all intelligent people that can find things out
for ourselves. We know enough to log onto the Internet and check what is in
this forum everyday. I don't see why a simple Internet search for this
service cannot be performed on any search engine. I performed a search on
Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in
my area. We coddle people in this country like they are helpless children, we
need to stop this or everybody will expect us to do everything for them.

Now I ask you do we really need this type of question in this forum?

If you all feel this is still rude I will unsubscribe.


From daemon Tue Feb 20 11:38:26 2001



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 20 Feb 2001 12:33:42 -0500 (EST)
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Wizard of the lab wrote:

} Now I ask you do we really need this type of question in this forum?
}
*** Yes. When I move to a new area, I ask colleagues to recommend a
dentist; I don't merely go to the yellow pages or the web and hope for the
best. In a similar vein, for most of us, our scopes are too precious to
let just anyone touch them; a recommendation from a satisfied colleague is
most valuable.

However, it would have been useful for the original poster to let us know
where he/she was located. People from across the world subscribe to this
list. A cleaning service in New Jersey may not be useful to the person in
California or Australia.

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Tue Feb 20 12:07:16 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Tue, 20 Feb 2001 13:06:58 -0500
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Fisher Scientific or ask a nearby university, department of biology. Good luck.



} } } {"Luvstodance99-at-aol.com"-at-sparc5.microscopy.com} 02/19 1:07 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We are searching for companies that provide cleaning and maintenance of
microscopes.

Rhonda Blassingill
Harrisonburg Cytology




From daemon Tue Feb 20 12:15:16 2001



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 20 Feb 2001 18:11:16 +0000
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These kinds of services are not in fact so easy to locate. Our
yellow pages would tell you nothing useful and web searches do
not provide a full list or carry any personal recommendation. The
question was simple, the reply can be equally simple or you can
simply delete and ignore it. The usefulness of a list like this lies in
the fact that even a simple question stands a high chance of being
quickly and appropriately answered, because the users have a
broad range interests and experience in common. Let's not
diminish this by inhibiting people from participating.

Chris

ps - who and where are you anyway?

}
} I am sorry you all are taking this so terrible, I was not intending to be
} rude. I was merely pointing out the fact that every time someone has a slight
} issue they want to magnify it in this forum. I think this forum can be used
} for better issues than this.
}
} It seems to me that we are all intelligent people that can find things out
} for ourselves. We know enough to log onto the Internet and check what is in
} this forum everyday. I don't see why a simple Internet search for this
} service cannot be performed on any search engine. I performed a search on
} Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in
} my area. We coddle people in this country like they are helpless children, we
} need to stop this or everybody will expect us to do everything for them.
}
} Now I ask you do we really need this type of question in this forum?
}
} If you all feel this is still rude I will unsubscribe.
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Tue Feb 20 12:34:20 2001



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Tue, 20 Feb 2001 10:29:40 -0800 (PST)
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, it is still rude. And it still doesn't address the issue of people
who live and work outside of major centres. And you are still anonymous.

Lesley Weston.



On Tue, 20 Feb 2001 Wizardofthelab-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am sorry you all are taking this so terrible, I was not intending to be
} rude. I was merely pointing out the fact that every time someone has a slight
} issue they want to magnify it in this forum. I think this forum can be used
} for better issues than this.
}
} It seems to me that we are all intelligent people that can find things out
} for ourselves. We know enough to log onto the Internet and check what is in
} this forum everyday. I don't see why a simple Internet search for this
} service cannot be performed on any search engine. I performed a search on
} Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in
} my area. We coddle people in this country like they are helpless children, we
} need to stop this or everybody will expect us to do everything for them.
}
} Now I ask you do we really need this type of question in this forum?
}
} If you all feel this is still rude I will unsubscribe.
}
}



From daemon Tue Feb 20 12:43:41 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 20 Feb 2001 12:37:05 -0600
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:25 AM 2/20/2001 -0500, you wrote:

{snip}

} It seems to me that we are all intelligent people that can find things out
} for ourselves. We know enough to log onto the Internet and check what is in
} this forum everyday. I don't see why a simple Internet search for this
} service cannot be performed on any search engine. I performed a search on
} Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in
} my area. We coddle people in this country like they are helpless children, we
} need to stop this or everybody will expect us to do everything for them.
}
} Now I ask you do we really need this type of question in this forum?

Perhaps you can enlighten some of the rest of us on the ease of searching.
I checked the Yahoo yellow pages for "microscope cleaning" and found
nothing in Iowa and not even anything around Chicago. I tried the regular
Yahoo and Google searches and found thousands of matches, but I was not
looking for a "coin cleaning microscope" or "cleaning a microscope slide".
In view of such irrelevant results, I am not surprised that someone would
ask the list for recommendations.

Perhaps you could give us a tutorial on how you were able to refine the
search to produce more useful results. I consider myself rather web savvy
and still fight the search engines. Such a tutorial could be a contribution
rather than so much noise.

I did find the service we use for cleaning our LMs in our local yellow
pages. It turns out to be through the distributor who first sold us the
scopes. However, if I were not in a university town, I doubt that there
would have been a listing.

} If you all feel this is still rude I will unsubscribe.

I don't know about rude, but I still have no idea of who you are. I don't
know what credentials or experience you might have that I should give any
consideration to your comments. An identity would help.

And in case anyone does not recognize iastate.edu as the domain for Iowa
State University...

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Tue Feb 20 13:05:17 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 20 Feb 2001 13:02:34 -0600
Subject: Re: Failure analysis of plastics and ceramics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris: have you tried the ASM International website?
(http://asminternational.org) Try the "Shop ASM" button at the top
right to see
their library of book, videos, etc. I'm looking at one now called
"Plastics
Failure Guide: Causes and Prevention" by Myer Ezrin. They also have
lots of
information on ceramics. You can also search for information. I'm a
member of
one of their affiliate societies, the Electronic Devices Failure
Analysis
Society. (You don't have to be a member to but from them, although it
is
cheaper.)

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
DSPS Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Tue Feb 20 14:06:43 2001



From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Tue, 20 Feb 2001 15:01:47 -0500
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't think it would hurt if you identified yourself. Your mask of
anonymity isn't helping matters much.

Gail Harrison
Reichhold

-----Original Message-----
} From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, February 20, 2001 11:26 AM
To: microscopy-at-sparc5.microscopy.com


I am sorry you all are taking this so terrible, I was not intending to be
rude. I was merely pointing out the fact that every time someone has a
slight
issue they want to magnify it in this forum. I think this forum can be used
for better issues than this.

It seems to me that we are all intelligent people that can find things out
for ourselves. We know enough to log onto the Internet and check what is in
this forum everyday. I don't see why a simple Internet search for this
service cannot be performed on any search engine. I performed a search on
Yahoo and found 1000+ results. I narrowed it down to an area and found 16 in

my area. We coddle people in this country like they are helpless children,
we
need to stop this or everybody will expect us to do everything for them.

Now I ask you do we really need this type of question in this forum?

If you all feel this is still rude I will unsubscribe.


From daemon Tue Feb 20 14:37:50 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 20 Feb 2001 14:34:04 -0600
Subject: RE: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Now I ask you do we really need this type of question in this forum?
}
} If you all feel this is still rude I will unsubscribe.

I think nobody relly wants to decrease number of listers,
but it's still not a chat room with all it's virtual emotions.

Wouldn't it be better if you would unsubscribe and
subscribe later under you real name?

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



From daemon Tue Feb 20 15:50:17 2001



From: Vachik Hacopian :      vhacopian-at-wellesley.edu
Date: Tue, 20 Feb 2001 16:44:37 -0500
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear "Wizardofthelab,"

Since you asked, yes, you were rude. No need to unsubscribe, though. Just
try to show common courtesy. If you don't care about a given question,
then don't reply. If you're interested in issues of self-reliant
individuals and non-coddling societies, you might take your own advice and
do a simple Internet search for an appropriate forum.

Vachik Hacopian

} I am sorry you all are taking this so terrible, I was not intending to be
} rude. I was merely pointing out the fact that every time someone has a
} slight
} issue they want to magnify it in this forum. I think this forum can be
} used
} for better issues than this.
}
} It seems to me that we are all intelligent people that can find things out
} for ourselves. We know enough to log onto the Internet and check what is
} in
} this forum everyday. I don't see why a simple Internet search for this
} service cannot be performed on any search engine. I performed a search on
} Yahoo and found 1000+ results. I narrowed it down to an area and found 16
} in
} my area. We coddle people in this country like they are helpless children,
} we
} need to stop this or everybody will expect us to do everything for them.
}
} Now I ask you do we really need this type of question in this forum?
}
} If you all feel this is still rude I will unsubscribe.





From daemon Tue Feb 20 16:12:10 2001



From: Wizardofthelab-at-aol.com
Date: Tue, 20 Feb 2001 17:08:25 EST
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am going to make you all happy and unsubscribe. You are making this exactly
what I was hoping to avoid.

You all sound like a bunch of whiny children.

Political correctness is ruining the fiber of this great country.

Enjoy your pathetic lives.

Mike Nolan
President
Materialographic Technologies

P.S. I am using a back-up ISP while my Primary ISP is working out some
problems and I did not realize my signature was not included.



From daemon Tue Feb 20 16:15:05 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 20 Feb 2001 14:14:28 -0800
Subject: Fwd: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rude language and anonymity are not appropriate at all. Another point of
view: ListServer is our public place for questions, answers and some
"social talking". This space we have to keep clean. If for some reason you
(or somebody else) don't like this place as it is- you may find many spaces
on the Internet, where your language and anonymity is welcomed. Just go to
the yellow pages...


} Date: Tue, 20 Feb 2001 11:25:34 -0500 (EST)
} From: "Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com
} Subject: Re: Cleaning and maintenance of microscopes
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: AOL 5.0 for Windows sub 117
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Feb 20 16:32:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 20 Feb 2001 14:26:12 -0800
Subject: Third party SEM maintenance sources

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looks like bad timing for this question on the list,
but the SEM won't wait.

Would appreciate leads on third party maintenance providers
for an Amray 1830 SEM located in Sacramento, CA. This system
has a Balzers 240 turbo, LaB6 gun, Varian 30L/s ion pump,
load lock system, and is currently on-line and working.

Providers of service contracts for this system are sought.
System is at a DOD facility.

tnx,
gary gaugler.



From daemon Tue Feb 20 16:45:31 2001



From: sghoshro-at-nmsu.edu
Date: Tue, 20 Feb 2001 15:40:58 -0700 (MST)
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think this kind of questions should always be welcome. The list serve is
here to entertain all kinds of microscopy related questions no matter how
simple it is. I posted a similar question last year and received some
excellent replies. I don't think yellow pages or internet search would
have done it. We are located in a small city and I can't imagine the
yellow pages will have any kind of microscopy services.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml






From daemon Tue Feb 20 17:48:28 2001



From: Louise_Harner-at-albint.com
Date: Tue, 20 Feb 2001 18:43:46 -0500
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The whole idea of this listserver is to synergistically combine the expertise of
people from a variety of backgrounds, locations, and experience levels so we can
assist each other. I suggest you rejoin those of us who (usually) quietly lurk
until you realize that flaming is neither helpful nor appropriate.

The person who requested assistance was thinking for herself - and doing it
well. After all microscopists are the best source of information on
individuals/companies truly qualified to clean microscopes. I do standard
cleaning/maintenance on my PLM's and stereomicroscopes at work and have cleaned
up many low end 'scopes as a freebie for public schools. But I wouldn't dream of
fully disassembling/cleaning some of the high end microscope systems I've used,
even if I had the tools available. I'd want a true expert - not just a name I
pulled from the yellow pages or internet.

Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com




"Wizardofthela
b-at-aol.com" To: microscopy-at-sparc5.microscopy.com
cc:
2001/02/19 Subject: Re: Cleaning and maintenance of microscopes
06:52 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Look in the phone book.

Why must we endure these mundane questions, can anybody in this world think
for themselves.






From daemon Tue Feb 20 19:12:45 2001



From: David Bentley :      dlb-at-email.arizona.edu
Date: Wed, 21 Feb 2001 00:04:07 -0600
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Rhonda and all list,

I'm an engineer in Nikon Japan. I used to work as sales engineer in NY.
How old and which maker's microscope do you mention? If it is Nikon,
could you contact our service or dealer? I know our service department
clean and maintain microscopes with reasonable charge.
Please contact 516-845-7788 first.

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp
----- Original Message -----
} From: {"Luvstodance99-at-aol.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 20, 2001 3:07 AM


No Great Loss.

Earl

----- Original Message -----
} From: {"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 20, 2001 2:08 PM


I am hesitant to reply to such a message, a few list members have
expressed much of my sentiments, but think that there is a deeper problem
with a few (very few but vocal) members of this list. I realize I am
preaching to the choir and those that should hear, won't. Attacks such as
this need to be discouraged.

Because of the quickness of a very few to attack without provocation,
usually my answers are restricted to off the list responses. This gets the
information to the inquirer, without requiring e-mail after e-mail in some
sort of battle. There have been useful lively conversations that I learned
much from, and gained deeper understanding, but these have always remained
civil, and dealt with the question at hand. Responses such as below,
really stifle I want from the list as a list member, a free flow of
questions and answers. These are what I learn from. There is a delete key
or trash bin for what I don't want to read, doesn't take long, nor much
effort. I have an enormous file of those I keep for future reference and
things I didn't know. The list works!

This list is for questions, hopefully for which, the inquirer doesn't
readily have the answer. What is to one, mundane and trivial, to others is
a significant question. Who is to chose what is mundane and for that
matter what is the scope of the inquiry? When read, I understood that
advice was being sought for the quality of service as well as who. This is
the kind of information that is available through this list. I am sorry I
wasn't able to respond to the original question but it was not from my
region, so I can't suggest good service engineers in the inquirer's area to
help.

I for one, would NOT want to trust a research light microscope worth
$30,000.00 or more to a choice from the Yellow pages (nor the Web for that
matter) without further information. We all have thrown bad service
personnel out of the lab, and had to pay the price of having the job done
right later. There is no information in the Yellow pages on how good or
how bad a given company is. Users of these services DO have this
information and we are the users.

As you send an e-mail, ask yourself, if the response you are giving
doesn't answer, or some way relate to, the question, why are you sending
it, and what good will it do for fellow list members?

Remember: There are no stupid questions, only stupid answers.

At 06:52 PM 2/19/2001 EST, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Feb 21 00:57:37 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Wed, 21 Feb 2001 17:53:27 +1100
Subject: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, I thought I'd see if I, too, could find 1000+ microscope maintenance
companies in the US, and there are a few out there. Search under
microscope maintenance:

www.southernmicro.com in Georgia
southernmicroscope.com/services.html somewhere in the South
www.dscoptical.com/services.htm near Boston
www.caleywhitmore.com near Boston
www.opticalexpertise.com in New York
www.mwrn.com/product/microscope/repair.htm lists various service companies
www.sciscope.com/field-service.htm based in Iowa, service most states
www.svms.com/services/index.html in silicon valley
www.meyerinst.com/html/mts/mts.htm in Texas
www.microresource.com/services2.htm in Illinois
www.allometrics.com/microscope_serv.htm in Lousiana and Texas
www.labworksinc.com in San Fransisco
www.biotherm-inc.com in 5 southern states
www.mikroni.com/fp/fp/services.htm in San Diego
www.uams.edu/oas/OES/oesilab.htm in Arkansas
www.uni.edu/pur/maintenance/equipment.html Uni of Northern Iowa
www.dominionmicroscope.com/services.htm Richmond, VA

etc.

took an hour to weed through them, while staining some sections......

cheers,




Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Wed Feb 21 02:28:41 2001



From: =?iso-8859-1?Q?J=F6rgen_Lennartsson?=
Date: Wed, 21 Feb 2001 09:20:50 +0100
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




----------------------------------------------------------------------------
------------

Jörgen Lennartsson

Marketing & Sales

Nanofactory Instruments AB
visitors address: Holtermansgatan 1C
Stena Center
SE 412 92 Göteborg
Sweden

tel. +46 31 772 81 77
mobile +46 705 30 06 97
fax +46 31 772 80 91
jorgen.lennartsson-at-nanofactory.com
{http://www.nanofactory.com}




From daemon Wed Feb 21 02:34:05 2001



From: Keith Ryan :      kpr-at-pml.ac.uk
Date: Wed, 21 Feb 2001 08:30:40 +0000
Subject: Re: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



To "Wizardofthelab"

If I can be of help - you don't seem to be great Wiz at "unsubscribing" - your wand needs to be aimed at:
ListServer-at-MSA.Microscopy.Com

for for this purpose - refer to the header of every message!

Keith Ryan

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Wed Feb 21 02:36:30 2001



From: pierre.frykberg-at-alfalaval.com
Date: Wed, 21 Feb 2001 09:29:13 +0100
Subject: Re: Failure analysis of plastics and ceramics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Chris Holp wrote:

"I would like to know if anyone can recommend any books regarding the
failure
modes and mechanisms of plastics and ceramics. My searches have been
yielded
little (read as nothing), and my library is woefully lacking.

Thanks to all in advance for your help!"

Chris Holp
ATC Materials Lab
holpcr-at-earthlink.net

I have been working with SEM since the beginning of the 80's and one of my
literature companions during all these years has been the excellent book
"An Atlas of Polymer Damage", ISBN 0 7234 0750 9. Regarding ceramics I
have little experience and do not know any good source.

Best regards

Pierre Frykberg
Alfa Laval Tumba AB
Div. Materials




From daemon Wed Feb 21 04:53:54 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Wed, 21 Feb 2001 04:54:00 -0600
Subject: RE: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Relax, and welcome to a public (though moderated) forum.

You got flamed because you failed to realize the extent of this list. I've
been a denizen for many years and have found questions and responses on
every level of expertise. As an internet resource, this list is
exceptional; however, you have to be responsible for your own filtering as
to what responses you make.

Basically, those posting here are interested primarily in constructive
information. Had you requested the original poster's location and provided
them with the results of your searches located within their area, there
would have been nothing left to argue. You would have been lauded as
helpful and the poster would have had the help they requested.

No whiney children here, only people of a wide variety of different
abilities and experience in this narrow field of knowledge who hope to find
some deeper understanding and practical help in fulfilling the tasks they
have to pursue each day.

I'm sorry if you really do leave this list. Having been in this field for
over 20 years, I rather enjoy the infusion of newcomers.

As an independent businessman, I am a staunch republican and arch-rival of
'political correctness', but I see none in this thread. A simple question
was asked, requiring a simple answer. If you didn't have that simple
answer, you should have just remained silent and deleted the email.
Instead, you chose to inject your displeasure that this particular email
was sent. Here's a surprise - this list is not maintained for your
pleasure, but for the practical use of hundreds, if not thousands, of
others.

You are welcome here, at least by me (I certainly can't speak for anyone
else here). Please recognize that you are preaching to the masses here,
and not just to an audience that you desire.

On Tuesday, February 20, 2001 4:08 PM,
"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com
[SMTP:"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com] wrote:
}
} I am going to make you all happy and unsubscribe. You are making this
exactly
} what I was hoping to avoid.
}
} You all sound like a bunch of whiny children.
}
} Political correctness is ruining the fiber of this great country.
}
} Enjoy your pathetic lives.
}
} Mike Nolan
} President
} Materialographic Technologies
}
} P.S. I am using a back-up ISP while my Primary ISP is working out some
} problems and I did not realize my signature was not included.
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Wed Feb 21 05:42:15 2001



From: Platek, Frank :      FPLATEK-at-ora.fda.gov
Date: Wed, 21 Feb 2001 06:38:08 -0500
Subject: List server "Flamers"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With regard to the recent "point / counter point" thread regarding the rude
postings of "the Wiz", I was initially reluctant to "add to the pile" when
so many had already voiced many of my sentiments. For years, I have
encouraged my SEM students at a local university to subscribe to "the List"
and regularly read and learn from the problems and questions many people
have shared with List members. If the students had specific questions or
similar problems, I have further encouraged them to post their comments or
questions. The most common response from students is "I don't DARE post my
silly question because everyone else will know the answer and I will look
foolish. I don't want to be put down or scolded before thousands of
knowledgeable readers" (I might add, who does?!) Until this recent thread
from "the Wiz", I could comfortably assure my students the list was a place
for good information without the assault of self appointed experts like "the
Wiz". The true damage a rude person like "the Wiz" does is to discourage
others from responding eventually leading to the death of the list server.

Problems like this one are inevitable. A few years ago, a forensic list
server was plagued with an even more blatant and as you would guess,
anonymous flamer. The flamer took delight in ridiculing many list members
and seeing how far he/she/they could upset the list members. The list
response was much the same as we are experiencing with "the Wiz". Our words
will not beat him/her/they back and their offer to unsubscribe is a joke.
Like a bully on a playground, if everyone ignores him/her, they will loose
interest and go away. If he/she/they cannot master to ability to delete
unwanted questions or text, how can we possibly expect them to abide by the
unpublished rules of good manners and mutual respect?

When I have learned EVERYTHING about ALL types of microscopy, I may see "the
Wizard's" view. But until then, I will enjoy and learn from fellow
microscopists and their responses to the MSA List server. Let's not waste
more personal (or company) resources on "the Wiz".

Keep up the good work, Nestor!

OBLIGATORY DISCLAIMER:
My comments and opinions expressed in the above text are mine alone and do
not represent those of the US FDA or any other Federal agency.


S. Frank Platek
Forensic Chemistry Center
U.S. Food and Drug Administration
6751 Steger Drive
Cincinnati, OH 45237-3097
(513) 679-2700 X254
fplatek-at-ora.fda.gov



From daemon Wed Feb 21 06:03:04 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 21 Feb 2001 08:24:24 -0500
Subject: Nestor, end this

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Seconded. There is no reason to unsubscribe.

} From: "Allen R. Sampson" {ars-at-sem.com}
Send reply to: "ars-at-sem.com" {ars-at-sem.com}
To: "'\"Wizardofthelab-at-aol.com\"-at-sparc5.microscopy.com'"
{"Wizardofthelab-at-aol.com"-at-sparc5.microscopy.com} ,
"microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}


Hi Listers,

Nestor, would you please call a halt to all the flying insults?

Everybody is getting their panties in a knot over this and I think it should end. Wizard was rude, but the replies are rude to.

To quote a "friend" of the LAPD ;-), "Can't we all just get along?"



Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed Feb 21 07:29:33 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 21 Feb 2001 07:27:08 -0600 (CST)
Subject: Tracking (C buildup from arcing) on an insulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


More years ago than I want to mention, whenever we changed
the filament on a Siemens 102 we cleaner the ceramic insulator
with some sort of polish to remove any C buildup. Does anyone
have a suggestion what to use as a "polish" for this.

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Feb 21 08:25:22 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 2/21/01 8:30 AM
Subject: Re: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When is enough, enough? The guy was harassed into unsubscribing.
Leave him alone now, please.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



To "Wizardofthelab"

If I can be of help - you don't seem to be great Wiz at "unsubscribing" - your w
and needs to be aimed at:
ListServer-at-MSA.Microscopy.Com

for for this purpose - refer to the header of every message!

Keith Ryan

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk






From daemon Wed Feb 21 09:18:37 2001



From: Kalman Rubinson :      kr4-at-nyu.edu
Date: Wed, 21 Feb 2001 10:13:20 -0500
Subject: Re: Wild M5 Eyepieces

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.3+Sun/8.9.1) id JAA13196
for dist-Microscopy; Wed, 21 Feb 2001 09:16:46 -0600 (CST)
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X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58



"RCHIOVETTI-at-aol.com"-at-sparc5.microscopy.com wrote:

} I'm not sure about this, but I recall someone telling me that the current
} Leica eyepieces for the MZ series of stereomicroscopes will also work on
the
} older Wild M5, M8, etc.

Also Olympus G series eyepieces work.

Kal



From daemon Wed Feb 21 10:00:37 2001



From: George Langford :      amenex-at-amenex.com
Date: Wed, 21 Feb 2001 11:00:03 -0500
Subject: Re: Cleaning and maintenance of microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello LOM'sts !

Now that a little of the invective has quieted down, here's
the contact information for John Sowers, who services Amenex's
light microscopes from his location in Claymont, Delaware:

J&L Microscope Services, (302) 798-5304

John has been servicing our B&L and Olympus microscopes for
about fifteen years; they still work as well or better than
they did when he started. What more can I say ?

Tell John I put you on to him.

Best regards,
George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
amenex-at-amenex.com
http://www.amenex.com/


From daemon Wed Feb 21 10:38:53 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 21 Feb 2001 16:44:23 +0000 (GMT Standard Time)
Subject: Re: Tracking (C buildup from arcing) on an insulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Laurence,

If it is a glazed insulator then we find that alumina
(Al2O3) powder mixed with alcohol to form a slurry is good.
Dipping a cotton bud in the slurry and working at the mark,
then a general clean all around the component with the
slurry on wipes is very effective. The alumina is easy to
clean off and does not leave any residue. It is very
important to wash thoroughly with frequent changes of
alcohol.

If the tracking is very bad (deep) then it may be necessary
to shot blast with alumina and then wash in alcohol. Shot
blasting also works for some of the porous insulators.

Good luck,
Ron


On Wed, 21 Feb 2001 07:27:08 -0600 (CST) "L. D. Marks"
{ldm-at-risc4.numis.nwu.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} More years ago than I want to mention, whenever we changed
} the filament on a Siemens 102 we cleaner the ceramic insulator
} with some sort of polish to remove any C buildup. Does anyone
} have a suggestion what to use as a "polish" for this.
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} -------------------------------------------------------
}
} Workshop May 17-19 2001 "New approaches to the Phase Problem"
} http://xraysweb.lbl.gov/esg/phasing/index.html
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Feb 21 10:50:54 2001



From: A. K. Christensen :      akc-at-umich.edu
Date: Wed, 21 Feb 2001 11:43:58 -0500
Subject: Re: List server "Flamers"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The bottom line is that "The List" should not be blamed for the opinion of
"The Wiz". Here in America we advocate free speech, and we should be used
to the idea that unpopular opinions will therefore occasionally be
expressed -- and we shouldn't overreact to them. "The List" is an open
forum, and so "The Wiz" has a perfect right to express his opinion. But
students and beginners should realize that most of us don't share that
opinion in this case, and they should feel perfectly free to ask questions
about anything they find confusing in the vast realm of microscopy. It
doesn't make any sense to me that anyone would abandon ship (unsuscribe)
just because an unpopular opinion was expressed. We need collectively to
have "thicker skins".

Kent Christensen

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 5801 Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Wed, Feb 21, 2001 6:38 AM -0500 "Platek, Frank" {FPLATEK-at-ora.fda.gov}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} With regard to the recent "point / counter point" thread regarding the
} rude postings of "the Wiz", I was initially reluctant to "add to the
} pile" when so many had already voiced many of my sentiments. For years,
} I have encouraged my SEM students at a local university to subscribe to
} "the List" and regularly read and learn from the problems and questions
} many people have shared with List members. If the students had specific
} questions or similar problems, I have further encouraged them to post
} their comments or questions. The most common response from students is
} "I don't DARE post my silly question because everyone else will know the
} answer and I will look foolish. I don't want to be put down or scolded
} before thousands of knowledgeable readers" (I might add, who does?!)
} Until this recent thread from "the Wiz", I could comfortably assure my
} students the list was a place for good information without the assault of
} self appointed experts like "the Wiz". The true damage a rude person
} like "the Wiz" does is to discourage others from responding eventually
} leading to the death of the list server.
}
} Problems like this one are inevitable. A few years ago, a forensic list
} server was plagued with an even more blatant and as you would guess,
} anonymous flamer. The flamer took delight in ridiculing many list members
} and seeing how far he/she/they could upset the list members. The list
} response was much the same as we are experiencing with "the Wiz". Our
} words will not beat him/her/they back and their offer to unsubscribe is a
} joke. Like a bully on a playground, if everyone ignores him/her, they
} will loose interest and go away. If he/she/they cannot master to ability
} to delete unwanted questions or text, how can we possibly expect them to
} abide by the unpublished rules of good manners and mutual respect?
}
} When I have learned EVERYTHING about ALL types of microscopy, I may see
} "the Wizard's" view. But until then, I will enjoy and learn from fellow
} microscopists and their responses to the MSA List server. Let's not waste
} more personal (or company) resources on "the Wiz".
}
} Keep up the good work, Nestor!
}
} OBLIGATORY DISCLAIMER:
} My comments and opinions expressed in the above text are mine alone and do
} not represent those of the US FDA or any other Federal agency.
}
}
} S. Frank Platek
} Forensic Chemistry Center
} U.S. Food and Drug Administration
} 6751 Steger Drive
} Cincinnati, OH 45237-3097
} (513) 679-2700 X254
} fplatek-at-ora.fda.gov
}






From daemon Wed Feb 21 10:51:03 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 21 Feb 2001 08:37:29 -0800
Subject: Amray maintenance sources (was third party sources)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 03:03 AM 2/21/01, you wrote:
} I don't know if it will help, but here's the response I had to make earlier
} today to a DOD request in the Sacramento area. The email address I was
} given didn't work, so I will have to call the party tomorrow. He claimed
} that they had three Amrays at their location. Amray's new service policies
} have certainly created some problems for customers!

Are any of you aware of a change in Amray's (KLA-Tencor) service
policies? It seems that their main problem is lack of trained personnel.
The second driver is the diminishing of importance of the lab SEMs
versus the KLA semiconductor fab/inspection equipment.

The problem with this particular 1830 system is that pre-contract inspection is
done on a per-diem basis. This is the lowest priority service call.
Systems are not placed on contract until the pre-inspection is done.
Sort of a Catch-22 situation.

This may not be a huge problem for systems like a 1000 or 1600
or 1610T. Maybe not too bad for an 1830. Non-Amray service
ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910,
3300 and 3600, it is quite another matter. Is there an impression
or actual set of data points where Amray/KLA is dropping the non-FESEMs
and retaining the FESEMs or simply dropping all models of SEMs?

Thanks,
gary gaugler



From daemon Wed Feb 21 11:50:00 2001



From: Battjes, Kevin :      battjes-at-impactanalytical.com
Date: Wed, 21 Feb 2001 12:44:55 -0500
Subject: Amray maintenance sources (was third party sources)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

KLA-Tencor, Amray Division recently sent me a notice they were no longer
providing service contracts for my area (Michigan). They also sent a list
of eleven third party service providers. We contracted with one of those
vendors for our service (Ex-Amray guys of course). The vendor we chose
simply accepted the instrument without inspection since we were just coming
off contract with KLA.

I can't speak for FESEMs since ours is an 1820 LaB6.

Good luck with your search.

Kevin

---------------------------------------------------------------
Kevin Battjes
Senior Research Specialist
Michigan Molecular Institute Voice 517-832-5555, ext 556
1910 W. St Andrews Road Fax 517-832-5560
Midland MI 48640 e-mail: battjes-at-mmi.org
After April 6, 2001 use 989 area code
---------------------------------------------------------------


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Wednesday, February 21, 2001 11:37 AM
To: ars-at-sem.com
Cc: MSA listserver


At 03:03 AM 2/21/01, you wrote:
} I don't know if it will help, but here's the response I had to make earlier
} today to a DOD request in the Sacramento area. The email address I was
} given didn't work, so I will have to call the party tomorrow. He claimed
} that they had three Amrays at their location. Amray's new service policies
} have certainly created some problems for customers!

Are any of you aware of a change in Amray's (KLA-Tencor) service
policies? It seems that their main problem is lack of trained personnel.
The second driver is the diminishing of importance of the lab SEMs
versus the KLA semiconductor fab/inspection equipment.

The problem with this particular 1830 system is that pre-contract inspection
is
done on a per-diem basis. This is the lowest priority service call.
Systems are not placed on contract until the pre-inspection is done.
Sort of a Catch-22 situation.

This may not be a huge problem for systems like a 1000 or 1600
or 1610T. Maybe not too bad for an 1830. Non-Amray service
ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910,
3300 and 3600, it is quite another matter. Is there an impression
or actual set of data points where Amray/KLA is dropping the non-FESEMs
and retaining the FESEMs or simply dropping all models of SEMs?

Thanks,
gary gaugler



From daemon Wed Feb 21 12:46:16 2001



From: Keith Collins :      collins-at-alrc.doe.gov
Date: Wed, 21 Feb 2001 10:39:43 PST
Subject: Re: Amray maintenance sources (was third party sources)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mr. Gaugler

To my knowledge KLA Tencor got out of the lab SEM business to include
service. They did not renew our service contract and supplied a list of third
party vendors . This is where I got Grant Gerringer's name.

I would suggest you contact AMRAY directly and get the list of third party
vendors and a copy of the letter.

781-275-1400
781-275-0740 fax
1-800-225-1462

Toll free may not work any more

Hope this helps

Good Luck
Keith Collins



From daemon Wed Feb 21 13:09:32 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 21 Feb 2001 14:05:03 -0500
Subject: Re: Tracking (C buildup from arcing) on an insulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







If it is a glazed insulator then we find that alumina
(Al2O3) powder mixed with alcohol to form a slurry is good.
Dipping a cotton bud in the slurry and working at the mark,
then a general clean all around the component with the
slurry on wipes is very effective. The alumina is easy to
clean off and does not leave any residue. It is very
important to wash thoroughly with frequent changes of
alcohol.

If the tracking is very bad (deep) then it may be necessary
to shot blast with alumina and then wash in alcohol. Shot
blasting also works for some of the porous insulators.


Dear Laurence,

If the shot-blasting is necessary, you may want to check out the Air
Eraser, available from Scientific Instrument Services. It's not expensive
and can operate on valved-down house compressed air. It has a small tip,
so it can easily be controlled.

Yours,

Bill Tivol




From daemon Wed Feb 21 13:09:32 2001



From: rad0 :      rden25-at-mindspring.com
Date: Wed, 21 Feb 2001 13:06:27 -0600
Subject: do sem's have a tech manual when new?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have aquired the remnants of an ISI-40 sem.

I'm new to this thing, and I'm going to need some sort
of technical manual just to identify the components.

So, do these exist? Did they ever?

And, if so, where might I start looking for them?

And also, this thing looks mighty old, is this model still used
by anyone, and are there technicians still alive who could work
on it?

Thanks,
Rick



From daemon Wed Feb 21 14:05:40 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Wed, 21 Feb 2001 12:00:51 -0800
Subject: re:microscope maintenance dialogue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Look at this dialogue as "theater-a la-internet" and we can see this in many different perspectives. Social commentary? Have we abdicated our ability to understand more than one perspective? It makes for great life art. I like to see it as an aggravation, a lesson, and humor....being in the audience...but then I love EM as art!




From daemon Wed Feb 21 14:56:48 2001



From: Kalman Rubinson :      kr4-at-nyu.edu
Date: Wed, 21 Feb 2001 15:50:52 -0500
Subject: Out of office SPAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


While we are expressing our feelings, let me strongly suggest that
subscribers temporarily unsubscribe if they are going to use an
autoanswer reply when out of the office.

If one posts to this list, one is addressing the entire list and a
stream of autoanswer SPAM should not result. Frankly, my dears, I don't
like getting autoanswer messages from people I do not know informing me
how long they will be away.

{end of rant}

Kal


From daemon Wed Feb 21 15:32:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 21 Feb 2001 13:14:56 -0800
Subject: x-ray inspection system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There was an earlier posting requesting information and
leads for x-ray inspection systems for integrated circuits.
I must have lost the post but I have found a recent
lead for what seems like a nice system. It is quoted in
the $75K price range as was at the requested budget amount
in the original posting.

Here is the info.

The Jewel Box by Glenbrook Technology, URL:

http://www.glenbrooktech.com/


gary g.



From daemon Wed Feb 21 16:34:45 2001



From: Alan Berginc :      aberginc-at-cressington.com
Date: Wed, 21 Feb 2001 16:28:50 -0600
Subject: passing of a colleague

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hate to be the bearer of bad news to the list but I feel many people
will want to know this.  After a long battle with cancer Terry
Donovan passed away yesterday. While his primary association with electron
microscopy was as a salesman he nonetheless had a vigorous enthusiasm for
the field and promoted it where ever he went. No doubt that over the years
he touched the lives of many people in a positive way, as he did mine.
Anyone who knew he was a sailing enthusiast may be interested to know he
wanted his ashes put into the Chesapeake Bay. Regards........Alan
Berginc Cressington Scientific, Inc 508 Thomson Park Drive Cranberry
Township, PA  16066-6425 TEL 724-772-0220 (USA)




From daemon Wed Feb 21 16:37:37 2001



From: jhaydenrbp :      jhaydenrbp-at-earthlink.net
Date: Wed, 21 Feb 01 17:39:25 -0500
Subject: Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am relatively new to this listserve and have been quietly monitoring
the various threads and wide array of responses. Although much has no
relevance to my work, I have found many responses quite useful and thank
the list for the opportunities it provides.

I am passing along a request for recommendations on image analysis
software. A client has a collection of fluorescence micrographs (film
originals) that they would like to analyze quantitatively. They do not
have a digital capture system that allows them to do the quantitation
real time. I believe it is a simple matter of thresholding and measurimg
the areas, but I may be missing something in my assumptions.

I have already recommended NIH (Scion) Image for the purpose, as I have
used an early version of that software for similar purposes, but they
would like to know if there are any highly recommended commercial
packages that might be better, or if there are any companies that could
do this analysis for them (again from their film originals).

The request comes from a graduate student at the University of Illinois
in Chicago who has money for this purpose.

Any suggestions either through the list or to me personally are greatly
appreciated and I will forward those responses on.

Thanks for your time.

James Hayden

*********************************

James E. Hayden, RBP, FBCA
Bio-Graphics
1058 Hemlock Drive
Blue Bell, PA 19422
tel/fax: (215)654-0625
tel:(215)514-4223

www.biographics.org

jhaydenrbp-at-earthlink.net





From daemon Wed Feb 21 18:24:42 2001



From: richardblack-at-cwcom.net
Date: Wed, 21 Feb 2001 19:33:00 -0600
Subject: Re: Wild M5 eyepieces

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Microscopy-at-sparc5.microscopy.com; Wed, 21 Feb 2001 16:12:54 -0800 (PST)


At 02:55 PM 2/21/01, you wrote:

(snip)
} This may not be a huge problem for systems like a 1000 or 1600
} or 1610T. Maybe not too bad for an 1830. Non-Amray service
} ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910,
} 3300 and 3600, it is quite another matter. Is there an impression
} or actual set of data points where Amray/KLA is dropping the non-FESEMs
} and retaining the FESEMs or simply dropping all models of SEMs?

The official word from Amray is that they are supporting the FESEM at this
time & will do so "for probably another five years".
As I do not have that in writing, the "five year" policy may change.

} Are any of you aware of a change in Amray's (KLA-Tencor) service
} policies? It seems that their main problem is lack of trained personnel.
} The second driver is the diminishing of importance of the lab SEMs
} versus the KLA semiconductor fab/inspection equipment.
}
} The problem with this particular 1830 system is that pre-contract
inspection is
} done on a per-diem basis. This is the lowest priority service call.
} Systems are not placed on contract until the pre-inspection is done.
} Sort of a Catch-22 situation.

I don't understand.
Are you trying to obtain a service contract from Amray at this time?
Or are you trying to obtain a contract from a "third party' maintenance
organization?

Regards,

Earl


----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: {ars-at-sem.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, February 21, 2001 8:37 AM


They do exist.

You might try "Aspex" (formerly RJ Lee Instruments) at (724) 744-0100.

Earl

----- Original Message -----
} From: "rad0" {rden25-at-mindspring.com}
To: "microscopers!!" {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, February 21, 2001 11:06 AM



I am fairly sure this is the case. I had a Leica MZ8 and MZ6 and the eyepieces
could be interchanged with the Wild M8 I had. I am not sure about tube lengths
and all that if such things are critical on stereo microscopes.

Richard Black.




From daemon Wed Feb 21 19:38:04 2001



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Wed, 21 Feb 2001 19:32:46 -0600
Subject: Liquid film thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi out there.

I have recently been approached by some of our researchers with the
request to assist them in developing an imaging system that enables them
to measure the thickness of a liquid and/or vapour film that develops
when water is sprayed onto a hot metal surface.

In approaching me with their request they hoped that my experience in
electron microcopy might give them some ideas. However, I am at a loss.


Can anyone out there give me advise whom to contact and what technique
has been developed to measure (in situ) liquid film thicknesses in the
order of approximately 30 microns.

Thanks

Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au




From daemon Wed Feb 21 23:21:57 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 22 Feb 2001 00:16:38 -0500
Subject: RE: Liquid film thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Optical techniques might work. Ellipsometry or other reflection techniques. Contact someone at JA Woollam in Lincoln, Nebraska.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Hans Brinkies [mailto:HBrinkies-at-groupwise.swin.edu.au]
Sent: Wednesday, February 21, 2001 8:33 PM
To: microscopy-at-sparc5.microscopy.com
Subject: Liquid film thicknesses


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
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---------------------------------------------------------------
--------.


Hi out there.

I have recently been approached by some of our researchers with the
request to assist them in developing an imaging system that
enables them
to measure the thickness of a liquid and/or vapour film that develops
when water is sprayed onto a hot metal surface.

In approaching me with their request they hoped that my experience in
electron microcopy might give them some ideas. However, I am at a loss.


Can anyone out there give me advise whom to contact and what technique
has been developed to measure (in situ) liquid film thicknesses in the
order of approximately 30 microns.

Thanks

Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au





From daemon Thu Feb 22 03:17:31 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 22 Feb 2001 03:14:35 -0600
Subject: RE: Amray maintenance sources (was third party sources)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been getting a lot of inquiries from Amray customers for contract
proposals, including one for a 3300 FESEM. In that case, I don't think
that Amray made any gestures - they also have a 1600 series SEM and are not
impressed with the service on the 3300 so we are talking about servicing
both.

Don't be too alarmed. Some of us independents are the most experienced EM
service personnel out there. I have over twenty years as an independent
working on all EM manufacturers as well as a wide variety of other
instruments such as XRF, AAS, UV-Vis, GC-MS, IR-FFT and a variety of
microprobes, not to mention my previous experience with an SEM
manufacturer, IBM and others.

The major concern for an FESEM owner transferring to an independent is, of
course, the cathode assembly. Amray will charge $10,000 for a swap and
billable installation on top of that. Denka, however, manufactures
emitters that will work in most FESEMs for generally less than $3,000 and
are available through Energy Beam Sciences. While there are increased
demands in reconditioning the electrostatic surfaces in an FESEM cathode,
they are easily managed. Given today's manufacturers service contract
pricing, you should be able to find an independent coming in at nearly 50%
less, not including the emitter replacements. Add in the purchase of one
emitter per year, and your costs should still be considerably lower, while
the experience level of your service engineer is increased.

Many FESEMs sold are located in companies that also have clean room
facilities or laminar flow hoods. Clean rooms only certify a certain
elimination of airborne particulates. While that is useful, it only
addresses one part of the contamination concerns regarding FE cathodes -
one that can be easily handled through a careful and methodical cathode
assembly under nearly any conditions.

The other concern is that of chemical cleanliness of the lens surfaces.
Once again, use of appropriate cleaning and handling methods can produce
good results. There is nothing magical here, more than likely the
manufacturers are doing the same work under conditions that may surprise
you, not to mention the experience and qualifications of the employees
doing the work.

Like a good jeweler or machinist, anyone experienced in servicing these
machines should be able to eyeball dimensions down to 1/10,000 of an inch,
perhaps with the help of a loupe. Alignment of any critical components
shouldn't be any problem.

Don't be shy to ask the questions you have of any independent you may be
considering. We survive on our integrity in a very limited field. If an
independent can't properly handle customer questions and problems, they
won't be able to feed their families for long. Believe me, it's tough to
keep a business like this going if you can't at least equal the service
capabilities of the manufacturer, as low a bar as that may be at times.

I can't tell you what Amray is considering other than the announcements
they have already made. I can tell you that similar moves were considered
by ISI prior to their withdrawal from the American market and subsequent
marketing and service licensing through another manufacturer. While such a
move is more than unlikely for Amray, I'd say that SEM manufacturers in
general are looking for creative ways to get out of self-imposed
difficulties.

It is possible that we are witnessing yet another new trend and other
manufacturers may be considering similar moves. The SEM field has grown
greatly over the past two decades, and manufacturers still seem unable to
recognize the true value of their service organizations - that of marketing
support by helping to build a base of loyal customers.

Instead, they are increasingly demanding their service organizations be
independent profit centers. In doing so, they bring a bean-counter
mentality to service that minimizes the concept of a career for service
engineers. By doing so, they encourage a rapid turn-over in their service
force which is why you probably have seen either a never ending stream of
promising, but inexperienced, engineers or a civil-service stereotypical
engineer who never quite makes the grade, but never leaves.

Sorry to have indulged in yet another of my tirades on the manufacturers.
Now and then there appears one manufacturer who seems to get it, and they
can profit greatly. There is one, who shall remain un-named, that I
recommended to my customers for over a decade because of the post sales
service they provided. I'm probably responsible for well over a million
dollars a year in sales for them for five to ten years.

I saw the writing on the wall when they announced lay-offs at their
corporate offices over five years ago; in a country where such a thing was
unheard of at the time. Their response seems to have been a clamp down on
expenditures and rising prices in foreign countries, witnessed by the
increasing requests I've received for service on their instruments over the
last few years. Frankly, I would have preferred to see them continue their
monopoly on good service, as I only give advice I am reasonable sure of.
However, having said that, I can state to everyone, as I stated in the
advice that I have given in the past, that every manufacturer eventually
f___s up.

Putting on the head dress of soothsayer, I can speculate that manufacturers
are looking to increase their profits by dumping those lines that produce
the least profit and concentrate on those lines that produce the most.
Having everyone who has bought into the latest and greatest of
technological advances, i.e. FE, snuckered into thinking that there are no
alternatives to their magic, there are greater profits for the
manufacturers in the service of these instruments. After all, the
alternative would seem to be a nearly doubling of their already bloated
service costs.

The trend over the last two decades has been to introduce new product
models at a rapid rate, regardless of any real evolutionary improvements.
These are due primarily to the increased influence of marketing
departments that seek not just to distance themselves from the competition,
but also from their own offerings. In this trend, manufacturers have
screwed themselves as the learning curve for inexperienced service
personnel for a large variety of models is much longer than is really
necessary. The result is that they expect more from their service
engineers and place increasing demands on them while not providing
increased incentives. I've yet to see a service engineer promoted beyond
service manager. Yet human resource, marketing and accounting underlings
are commonly promoted to top corporate positions. Why stay in service?

I've seen the industry standard service contract cost rise from 5% of
purchase price per year to over 10%. I have not seen any indication that
those costs are justified, except through poor management decisions by the
manufacturers. Since the purchase prices themselves reflect any basic cost
increases over the years, exactly how do you justify the doubling of
service costs which I've stated in proportion to the purchase price?

Have I given you a hint of where I'm coming from? Sorry, but sometimes
when you give me a nail, I'll grab a 16 pound sledge hammer, although never
when I'm working on an SEM. However, you may want to bolt your windows, as
I often want to toss an instrument out. (To Fred, and anyone else who
tends to take me too seriously, the immediately preceding was intended as a
joke, as poor as it was).

On Wednesday, February 21, 2001 10:37 AM, Gary Gaugler
[SMTP:gary-at-gaugler.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 03:03 AM 2/21/01, you wrote:
} } I don't know if it will help, but here's the response I had to make
earlier
} } today to a DOD request in the Sacramento area. The email address I was
} } given didn't work, so I will have to call the party tomorrow. He
claimed
} } that they had three Amrays at their location. Amray's new service
policies
} } have certainly created some problems for customers!
}
} Are any of you aware of a change in Amray's (KLA-Tencor) service
} policies? It seems that their main problem is lack of trained personnel.
} The second driver is the diminishing of importance of the lab SEMs
} versus the KLA semiconductor fab/inspection equipment.
}
} The problem with this particular 1830 system is that pre-contract
inspection is
} done on a per-diem basis. This is the lowest priority service call.
} Systems are not placed on contract until the pre-inspection is done.
} Sort of a Catch-22 situation.
}
} This may not be a huge problem for systems like a 1000 or 1600
} or 1610T. Maybe not too bad for an 1830. Non-Amray service
} ought to be fine. But for FESEMs like 1840, 1845, 1880, 1910,
} 3300 and 3600, it is quite another matter. Is there an impression
} or actual set of data points where Amray/KLA is dropping the non-FESEMs
} and retaining the FESEMs or simply dropping all models of SEMs?
}
} Thanks,
} gary gaugler
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Feb 22 03:41:20 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 22 Feb 2001 03:42:37 -0600
Subject: RE: do sem's have a tech manual when new?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are quite a few ISI-40s still in use. As a basic SEM, it works. ISI
made their inroads in the American market as a low bidder, offering a
useable instrument at a price that others couldn't match.

There are a variety of independent service providers out there that can
service your instrument, but you need to specify your location.

The last I knew, ISI made their schematics available at an incredibly low
cost (I got mine at $35 US). Access to ISI is currently through Leo (have
I got it right?) and you should contact their parts department for the
schematics. Their schematics are in Japanese (or is it Korean?) so you
should be cognizant of international electronic symbols and nomenclature.
If not, then you should seek the help of someone who is. As far as I am
aware, the electrical schematics are all that is available.

On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I have aquired the remnants of an ISI-40 sem.
}
} I'm new to this thing, and I'm going to need some sort
} of technical manual just to identify the components.
}
} So, do these exist? Did they ever?
}
} And, if so, where might I start looking for them?
}
} And also, this thing looks mighty old, is this model still used
} by anyone, and are there technicians still alive who could work
} on it?
}
} Thanks,
} Rick
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Feb 22 06:05:30 2001



From: =?iso-8859-1?Q?Agn=E9s?= de Matteis :      agnes.matteis-at-EMBL-Heidelberg.de
Date: Thu, 22 Feb 2001 13:00:07 +0100
Subject: Meeting & Workshop announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to announce the 1st International Meeting & Workshop on
Advanced Light Microscopy - June 6-10, 2001 - Santa Maria Imbaro, Italy.

The program will include lectures on recent exciting advances in the fields
of light microscopy and their applications to modern questions in life
sciences.
The practical workshop (afternoon), organised by manufacturers, leading in
the field, will give the possibility to learn practical aspects on the
latest developments in the field.
Evening session will give students the possibility to discuss their own
work and we also will have round table discussions bringing together
manufacturers and scientists discussing where light microscopy presently
stands and where it should move to in the future.

For further information, please check this web page:
http://www.embl-heidelberg.de/ELMI/ItalyMeeting




Agnès de Matteïs
Assistant Visitors Programme/EURALMF (room 415)
Tel: + 49 6221 387 138
Fax: + 49 6221 387 512
matteis-at-embl-heidelberg.de




From daemon Thu Feb 22 06:13:28 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 22 Feb 2001 04:13:56 -0600
Subject: RE: do sem's have a tech manual when new?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Having dug further into my emails for the day, Earl has provided the right
answer, contrary to my previous posting. I swear, its getting harder every
day to track the passage of individual SEM manufacturers. RJ Lee did
indeed handle the ISI assets in the US, my mistake, and you should contact
them for info. The transfer to Aspex was not known to me, but web links to
RJ Lee as a manufacturer appear to be non-existent and Earl is probably
right. I'm confused...

On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I have aquired the remnants of an ISI-40 sem.
}
} I'm new to this thing, and I'm going to need some sort
} of technical manual just to identify the components.
}
} So, do these exist? Did they ever?
}
} And, if so, where might I start looking for them?
}
} And also, this thing looks mighty old, is this model still used
} by anyone, and are there technicians still alive who could work
} on it?
}
} Thanks,
} Rick
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Feb 22 07:18:59 2001



From: Chris Holp :      holpcr-at-earthlink.net
Date: Thu, 22 Feb 2001 08:18:26 -0500
Subject: Responses to failure of plastics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you all for the myriad of responses to the inquiry regarding failures
in plastics and ceramics. Below is a brief compilation of suggested sources:

"Medical Plastics: Degradation Resistance & Failure Analysis", Portnoy, ISBN
1-884207-60-X
"Plastics Failure Guide: Cause & Prevention", Ezrin, ISBN 1-56990-184-8
(These two are available at the www.asm-intl.org site)

"An Atlas of Polymer Damage" ISBN 0-7234-0750-9
"Failure of Plastics", Brostow & Corneliussen, ISBN 3-446-14199-5
"Polymer Microscopy" ISBN 0-412-25710-6
"An Atlas of Polymer Damage", Engle et al, ISBN 0-13-050013-5
"Polymer Degradation", W. Schnabel
"Case Studies of Plastics Design & Failure Analysis", Mallick/Ford Motor Co.
"Handbook of Plastics Technology", SPE
"Polymer Characterization & Analysis", Kroschwitz
"Handbook of Plastics Flammability", Landrock
"Handbook of Plastics Degradation", Mamid & Amin

This is a quick list and I have not yet investigated each title, but I do
have a few on order. I was actually impressed at the number of relevant to
semi-relevant books shown at Amazon.com. Also, The Society of Plastics
Engineers has a nice site with good book offerings.

Chris Holp
ATC Materials Lab
Cleveland, OH
holpcr-at-earthlink.net






From daemon Thu Feb 22 07:36:44 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 22 Feb 2001 08:27:24 -0800
Subject: Re: Tracking (C buildup from arcing) on an insulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Access is through Aspex.
Leo is a consortium of Zeiss & Cambridge.


----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'rad0'" {rden25-at-mindspring.com} ; "microscopers!!"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 22, 2001 1:42 AM


Dear Laurence,
I have used six micron diamond polishing paste for this, in the past. It
must be very thoroughly washed off with clean ethanol or methanol.
At 07:27 AM 2/21/01 -0600, you wrote:
} More years ago than I want to mention, whenever we changed
} the filament on a Siemens 102 we cleaner the ceramic insulator
} with some sort of polish to remove any C buildup. Does anyone
} have a suggestion what to use as a "polish" for this.
}
} -------------------------------------------------------
} Laurence Marks
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Feb 22 11:16:03 2001



From: Tom W Bargar :      tbargar-at-unmc.edu
Date: Thu, 22 Feb 2001 11:12:20 -0800
Subject: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm having a stabiliy problem with LR White sections. They curl, expand,
split, etc. during examination on the TEM. My operating conditions are
60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
low. In the past I've tried vac. evap. a layer of carbon which helped some.
I was also thinking of coating the grids with another layer of formvar to
"sandwich" the sections. I would appreciate anyone's thoughts and what they
have tried in solving the stability problem. thanks.

Tom Bargar
EM Lab, UNMC
Omaha, NE
(402)559-7347



From daemon Thu Feb 22 11:48:24 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 22 Feb 2001 11:43:46 -0600
Subject: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom,

We've had nothing but trouble with LR White and have finally switched to
Unicryl to see if that improves things. Not only has LRW been unstable
under the beam, but we've had repeated problems with sections being
destroyed in the immunolabeling and staining processes. We have cleaned and
recleaned our grids. We have tried different hardness grades of LRW and
different batches of resin. We've tried extended dehydrations and
infiltrations and abbreviated ones, from the manufacturer's suggested
protocol to every sensible variation we could think of. The final straw was
when we processed LRW and Unicryl embedded samples side by side and got
beautiful stable sections in Unicryl and nonexistent to tattered shreds on
the LRW grids.

We're still pretty new to Unicryl, so I'm sure it will have its own set of
problems, but so far it's been more than acceptable.

There are some archived discussions about LR resins at
http://www.biotech.ufl.edu/~emcl/tips.html.

Meanwhile, I'd be interested in seeing any responses you get, if you
wouldn't mind.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Tom W Bargar [mailto:tbargar-at-unmc.edu]
Sent: Thursday, February 22, 2001 1:12 PM
To: Microscopy-at-sparc5.microscopy.com


I'm having a stabiliy problem with LR White sections. They curl, expand,
split, etc. during examination on the TEM. My operating conditions are
60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
low. In the past I've tried vac. evap. a layer of carbon which helped some.
I was also thinking of coating the grids with another layer of formvar to
"sandwich" the sections. I would appreciate anyone's thoughts and what they
have tried in solving the stability problem. thanks.

Tom Bargar
EM Lab, UNMC
Omaha, NE
(402)559-7347



From daemon Thu Feb 22 12:42:16 2001



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Thu, 22 Feb 2001 12:39:41 -0600
Subject: Teorell-StenhagenBuffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listservers,

I'm trying to track down a
formulation for Teorell and
Stenhagen buffer. A 2.5mM
concentration of diaminobenzidine
is used with this buffer and
hydrogen peroxide to selectively
stain the catalase in peroxisomes.
The references that I have found
all seem to use this buffer and
reference an article in a German
journal dating to 1938.
Is anyone out there familiar with
this buffer? If so, can you supp;y
the recipe? Is there another buffer
that I can use to effectively stain peroxisomes?
As always, thanks in advance for
your replies.

Tom Januszewski
Senior Electron Microscopist
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
FAX: 214-648-6408
Email: tom.januszewski-at-UTSouthwestern.edu


From daemon Thu Feb 22 13:30:35 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Thu, 22 Feb 2001 12:25:42 -0700 (MST)
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom - Have you tried sort of "burning" the sections at low mag, low beam
for a few minutes when they first go in the beam? That may help stabilize
them for work at higher mags. That has helped me in the past.

However, I'm joining the Unicryl camp :) Never had trouble with losing LR
White sections during processing, but the few times I've used Unicryl so
far I've had less trouble with microholes in the sections (had those in LR
Gold, too) and the sections have been more stable in the beam.

Not bashing LR resins - they've worked just fine for me for years, but I'm
all for trying newer stuff when I can!

Tamara

On Thu, 22 Feb 2001, Tom W Bargar wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm having a stabiliy problem with LR White sections. They curl, expand,
} split, etc. during examination on the TEM. My operating conditions are
} 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
} low. In the past I've tried vac. evap. a layer of carbon which helped some.
} I was also thinking of coating the grids with another layer of formvar to
} "sandwich" the sections. I would appreciate anyone's thoughts and what they
} have tried in solving the stability problem. thanks.
}
} Tom Bargar
} EM Lab, UNMC
} Omaha, NE
} (402)559-7347
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Thu Feb 22 13:48:39 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Thu, 22 Feb 2001 11:44:56 -0800
Subject: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,
I use routinely formvar/carbon coated copper grids. The other way of support I
use, especially for high resolution work and elemental analysis where I don't
want to look through the layer of carbon, is a holey "lacey" carbon film. The
support is superb, the drawback is a limited viewing area. I purchase both from
EMS. I've never tried to make a sandwich with another formvar layer, but sounds
to me kind of labor-intensive (not speaking of another layer you'd be imaging
through). Good luck, Alice.

Alice Dohnalkova
Battelle, PNNL
MS P7-50
Richland, WA 99352
(509) 372-0692

-----Original Message-----
} From: Tom W Bargar [mailto:tbargar-at-unmc.edu]
Sent: Thursday, February 22, 2001 11:12 AM
To: Microscopy-at-sparc5.microscopy.com


I'm having a stabiliy problem with LR White sections. They curl, expand,
split, etc. during examination on the TEM. My operating conditions are
60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
low. In the past I've tried vac. evap. a layer of carbon which helped some.
I was also thinking of coating the grids with another layer of formvar to
"sandwich" the sections. I would appreciate anyone's thoughts and what they
have tried in solving the stability problem. thanks.

Tom Bargar
EM Lab, UNMC
Omaha, NE
(402)559-7347



From daemon Thu Feb 22 15:54:27 2001



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Thu, 22 Feb 2001 13:48:02 -0800
Subject: RE: do sem's have a tech manual when new?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


RJ Lee Group had spun off RJ Lee Instruments a couple of years ago. RJ Lee Instruments, Limited changed it's name to Aspex, LLC last year. ASPEX stands for Application Specific Products using Electron
beam and X-ray analytical technologioes, they tell me. It was easier for me to remember RJ Lee. For more info their web address is www.aspexllc.com . Which has some contact information or a button for questions as I recall. If ASPEX doesn't handle what you want I'm sure they can give you contact information for RJ Lee Group.

Jim Roberts


James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "Allen R. Sampson" {ars-at-sem.com} 02/22/01 02:13AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Having dug further into my emails for the day, Earl has provided the right
answer, contrary to my previous posting. I swear, its getting harder every
day to track the passage of individual SEM manufacturers. RJ Lee did
indeed handle the ISI assets in the US, my mistake, and you should contact
them for info. The transfer to Aspex was not known to me, but web links to
RJ Lee as a manufacturer appear to be non-existent and Earl is probably
right. I'm confused...

On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I have aquired the remnants of an ISI-40 sem.
}
} I'm new to this thing, and I'm going to need some sort
} of technical manual just to identify the components.
}
} So, do these exist? Did they ever?
}
} And, if so, where might I start looking for them?
}
} And also, this thing looks mighty old, is this model still used
} by anyone, and are there technicians still alive who could work
} on it?
}
} Thanks,
} Rick
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092







From daemon Thu Feb 22 16:31:44 2001



From: muller.bruce-at-columbus.co.za ()
Date: Thu, 22 Feb 2001 16:17:33 -0600
Subject: Ask-A-Microscopist:deep-etch Type 316 SS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(muller.bruce-at-columbus.co.za) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, February
22, 2001 at 06:28:29
---------------------------------------------------------------------------

Email: muller.bruce-at-columbus.co.za
Name: Bruce Muller

State: Mpumalanga

Zip: 1050

Question: What solution and method would one use to deep-etch Type 316
STAINLESS STEEL to reveal the inclusions and/or precipitates present in
relief. This will be used for SEM analysis.
Thankyou in advance for your assistance.
Bruce

---------------------------------------------------------------------------




From daemon Thu Feb 22 16:50:45 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Thu, 22 Feb 2001 16:55:36 -0600
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
I think there are a few things you should be careful with. First, don't use
naked grids with LRW sections, always use film supported grids. Second, don't
try to use chloroform to extend the wrinkles of your sections before picking
them up. Third, don't use UAc in EtOH or MtOH, use aquatic UAc for your
staining.

Good luck

Greg Ning
EM Facility
Medical College of Wisconsin

Tom W Bargar wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm having a stabiliy problem with LR White sections. They curl, expand,
} split, etc. during examination on the TEM. My operating conditions are
} 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
} low. In the past I've tried vac. evap. a layer of carbon which helped some.
} I was also thinking of coating the grids with another layer of formvar to
} "sandwich" the sections. I would appreciate anyone's thoughts and what they
} have tried in solving the stability problem. thanks.
}
} Tom Bargar
} EM Lab, UNMC
} Omaha, NE
} (402)559-7347



From daemon Thu Feb 22 16:55:28 2001



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 22 Feb 2001 17:53:57 -0500
Subject: Si wafers wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Can someone please give me a source for free or inexpensive Si wafer
rejects? I break them into small squares to use as SEM substrates for nano
particles.
Thank you,
Jim

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Feb 22 18:18:03 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Fri, 23 Feb 2001 10:14:51 +1000
Subject: Re: do sem's have a tech manual when new?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The company that now owns ISI is the Optical company TOPCON they have
branches through out the world and locally Topcon still has "ISI"
instruments available.
Regards
JVN

"Allen R. Sampson" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} There are quite a few ISI-40s still in use. As a basic SEM, it works. ISI
} made their inroads in the American market as a low bidder, offering a
} useable instrument at a price that others couldn't match.
}
} There are a variety of independent service providers out there that can
} service your instrument, but you need to specify your location.
}
} The last I knew, ISI made their schematics available at an incredibly low
} cost (I got mine at $35 US). Access to ISI is currently through Leo (have
} I got it right?) and you should contact their parts department for the
} schematics. Their schematics are in Japanese (or is it Korean?) so you
} should be cognizant of international electronic symbols and nomenclature.
} If not, then you should seek the help of someone who is. As far as I am
} aware, the electrical schematics are all that is available.
}
} On Wednesday, February 21, 2001 1:06 PM, rad0 [SMTP:rden25-at-mindspring.com]
} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello,
} }
} } I have aquired the remnants of an ISI-40 sem.
} }
} } I'm new to this thing, and I'm going to need some sort
} } of technical manual just to identify the components.
} }
} } So, do these exist? Did they ever?
} }
} } And, if so, where might I start looking for them?
} }
} } And also, this thing looks mighty old, is this model still used
} } by anyone, and are there technicians still alive who could work
} } on it?
} }
} } Thanks,
} } Rick
} }
} }
} }
}
} Allen R. Sampson, Owner
} Advanced Research Systems
} 317 North 4th. Street
} St. Charles, Illinois 60174
} voice 630.513.7093 fax 630.513.7092

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Thu Feb 22 21:49:03 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 22 Feb 2001 19:46:48 -0800
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, Tom

I would pick up sections on the carbon-plastic-coated grid and then put
second layer of carbon over. In such "sandwich", extra couple of
nanometers of carbon does not harm your resolution. Carbon should work
perfectly to protect your sections. Secondly, I would try higher HT, like
80 kV.

Good luck,
Sergey

At 11:12 AM 2/22/01 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Feb 22 21:53:45 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Fri, 23 Feb 2001 14:49:27 +1100
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom,

A few years ago I did quite a bit of immunogold labelling on LR White
sections on uncoated 400 mesh copper thin-bar grids (washed thoroughly with
acetone) at 80-100 kV and imaging at 40-100K. I usually "baked" the
sections a bit at low mag before imaging at high mag - as suggested by
Tamara Howard. They were rather unstable, but I would usually be able to
count gold particles and/or take a photo before the section blew up. As
you say, keeping the beam intensity low prolonged their life a bit, enough
to get the information needed. So I didn't solve the problem, but just
worked around it as much as possible. Also, I cut thickish sections - dark
gold, expanding to pale gold with chloroform vapour waved over the knife
bath. Longer polymerisation might help, but then you may lose antibody
access to the tissue, if that's what you're interested in. I used medium
or hard grade resin polymerised ~16 hours at 55 C. Over 4 years, we did
have a couple of dud batches that didn't polymerise very well.

good luck,
Rosemary White
}
} I'm having a stabiliy problem with LR White sections. They curl, expand,
} split, etc. during examination on the TEM. My operating conditions are
} 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
} low. In the past I've tried vac. evap. a layer of carbon which helped some.
} I was also thinking of coating the grids with another layer of formvar to
} "sandwich" the sections. I would appreciate anyone's thoughts and what they
} have tried in solving the stability problem. thanks.
}
} Tom Bargar
} EM Lab, UNMC
} Omaha, NE
} (402)559-7347



Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Thu Feb 22 21:55:10 2001



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Fri, 23 Feb 2001 14:51:48 +1100
Subject: Leitz DURIMET

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The objectives of our Leitz DURIMET Micro-Hardness tester was damaged
during class experiments last year. Has anyone an idea where I can
purchase a x10 (A 0.18 C HM25~) and a x40 (A 0.7 C HM6.3~) objective
that suits a Leitz DURIMET.

Thanks



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Thu Feb 22 23:00:23 2001



From: asys.hitech-at-zdnetmail.com.br
Date: Mon, 19 Feb 2001 17:09:10 -0600
Subject: Dow Jones Investment Video for you 30578

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Thu Feb 22 23:54:59 2001



From: Dale Shumaker :      dshumake-at-mail.jhmi.edu
Date: Fri, 23 Feb 2001 00:48:31 -0500 (EST)
Subject: Re: Si wafers wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Depending on what you call cheap, Ted Pella sells 4" wafers precut to either
5x7 or 5x5mm chips for $61.00 US.


Dale

On Thu, 22 Feb 2001, JIM ROMANOW wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} Can someone please give me a source for free or inexpensive Si wafer
} rejects? I break them into small squares to use as SEM substrates for nano
} particles.
} Thank you,
} Jim
}
} James S. Romanow
} The University of Connecticut
} Physiology and Neurobiology Department
} Electron Microscopy Facility
} Unit-2131
} Storrs, CT 06269-2131
} bsgphy3-at-uconnvm.uconn.edu
} 860 486-2914 voice
} 860 486-1936 fax
}
}
}

Dale Shumaker
G9 WBSB
725 N Wolfe Street
Baltimore, MD 21205

Wilson Lab; Johns Hopkins University School of Medicine,
Cell Biology and Anatomy
410-614-2654
410-955-4129 (fax)



From daemon Fri Feb 23 00:49:46 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Fri, 23 Feb 2001 07:45:35 +0100
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
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Hi Tom!
LR-White behaves sometimes a little bit "excentric" ...
I would like to give you some hints drawn from my personal experience with LR-White:

-I use medium grade LR-W. That´s not because I tried other grades, but this works well with me

- The way of LRW polymerization is critical. If you do it in the heat, at 55-60°C, with or without vacuum, plastic gets very hard and sometimes brittle. Although, it´s easy to cut but the sections tend to be unstable in the beam, they suffer badly during immunoprocedures (sometimes only 1 out of 4 survives...), they are destroyed by alcoholic UA, contrast is sometimes awfully low and so on ...

- I polymerize LR-White in the cold at -20° by addition of 15microlitres of accelerator to 1ml of fresh resin which is in good-sealing microtubes (air prevents polymerization). What I get is not that good to cut because is softer than heat-polymerized LR-W, but it comes off the knife without wrinkles, is very stable during processing and in the beam at even 80kV. Contrast is sufficient, even if you only stain with (aquaeous) Uranyl and omit the lead. Immunolabeling works fine if you add 0.025 % Tween-20 to washing buffer and secondary gold-probe.

- Yes its true, the older this stuff gets even in your fridge, the poorer polymerization (especially by the use of the accelerator) will be. One year should be a batch´s life more or less.

- I use simply formvar coated nickle/copper grids. That is sufficient. If you have the facilities to coat the grids with carbon, it might work even better, everything is more robust during immunolabeling.

Why don´t you try it with the accelerator. Important: be quick as polymerization sets on immeadiatly, for that cool down the resin together with the samples (-20°C), don´t polymerize more than a handful of samples at one time. Use some kind of plastic microtubes (eppendorf, sarstedt and so on ...) which seal tight! After adding accelerator to the cold resin, shake vigorously and than hurry up. Make a paper copy of sample numbers and use them, laser-printouts get smeary and unreadable after LRW polymerziation.

If you have further questions, don´t hesitate to contact me.

Good luck,
Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I

} I'm having a stabiliy problem with LR White sections. They curl, expand,
} split, etc. during examination on the TEM. My operating conditions are
} 60KV, Magnifcations in range of 10 to 45K, trying to keep beam intensity
} low. In the past I've tried vac. evap. a layer of carbon which helped some.
} I was also thinking of coating the grids with another layer of formvar to
} "sandwich" the sections. I would appreciate anyone's thoughts and what they
} have tried in solving the stability problem. thanks.
}
} Tom Bargar
} EM Lab, UNMC
} Omaha, NE
} (402)559-7347

_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Fri Feb 23 07:50:23 2001



From: FARNHAM, WARREN H :      WHFARN-at-solutia.com
Date: Fri, 23 Feb 2001 08:42:00 -0500
Subject: RE: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
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Have you tried making carbon/metal grids (Collodion or Forvar
removed). I'm wondering if the metal might help dissipate the heat.
Additionally, can you increase your HT (even though the potential
energy is higher) which will decrease wavelength which I have found
helps with beam sensitive materials. Although, maybe you require low
KV for contrast.


Warren


From daemon Fri Feb 23 08:34:06 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 23 Feb 2001 09:30:49 -0500
Subject: Speaking of Ted Pella's Si precut wafers

Contents Retrieved from Microscopy Listserver Archives
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I have been using Pella's precut wafers for making cross section samples. I only use them as blanks to build up the cross section. They come with an adhesive sheet that binds them together. When I get further into the middle of the wafer, the edges of the piece have a tendency to chip because of the adhesive. Does anyone know how to release them without this occurring. I am concerned about solvents contaminating them and preventing adhesion.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Dale Shumaker [mailto:dshumake-at-mail.jhmi.edu]
Sent: Friday, February 23, 2001 12:49 AM
To: JIM ROMANOW
Cc: microscopy-at-sparc5.microscopy.com
Subject: Re: Si wafers wanted


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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ListServer-at-MSA.Microscopy.Com
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---------------------------------------------------------------
--------.


Depending on what you call cheap, Ted Pella sells 4" wafers
precut to either
5x7 or 5x5mm chips for $61.00 US.


Dale

On Thu, 22 Feb 2001, JIM ROMANOW wrote:

}
---------------------------------------------------------------
---------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
}


---------------------------------------------------------------
--------.
}
}
} Hello,
} Can someone please give me a source for free or inexpensive Si wafer
} rejects? I break them into small squares to use as SEM
substrates for nano
} particles.
} Thank you,
} Jim
}
} James S. Romanow
} The University of Connecticut
} Physiology and Neurobiology Department
} Electron Microscopy Facility
} Unit-2131
} Storrs, CT 06269-2131
} bsgphy3-at-uconnvm.uconn.edu
} 860 486-2914 voice
} 860 486-1936 fax
}
}
}

Dale Shumaker
G9 WBSB
725 N Wolfe Street
Baltimore, MD 21205

Wilson Lab; Johns Hopkins University School of Medicine,
Cell Biology and Anatomy
410-614-2654
410-955-4129 (fax)




From daemon Fri Feb 23 08:44:06 2001



From: csedax-at-alpha.arcride.edu.ar
Date: Fri, 23 Feb 2001 11:42:54 -0300
Subject: Thanks about the asbestos analysis for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi dear microscopists,

we appreciate very much all responses about our
request related to a negative analysis of asbestos in building
materials.
We still might ask some of you "off line" for details or further
comments.
Thanks a lot!

Silvia Montoro
Centro Regional de Investigaciones y Desarrollo de Santa Fe
Santa fe
Argentina


From daemon Fri Feb 23 10:03:43 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 23 Feb 2001 10:58:37 -0500
Subject: RE: Teorell-StenhagenBuffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom:

Try: Roels, F, et al: J. Histochem. Cytochem. 22:442-4,1974
Roels, F, et al: J. Histochem. Cytochem. 27: 1471-7, 1979
Roels, F, et al: Am. J. Med. Genet. 25:257-71, 1986


One of these might have the recipe, however, I have used a procedure
published by De Craemer, et al in the Journal of Histochemistry and
Cytochemistry, 17:675-680, 1969. They use Tetra-HCl buffer which is more
commonly called Tris-HCl buffer. I labeled catalase in liver peroxisomes.
I immersion fixed 2mm thick sections of liver 6 hours in 3% Glut
made up in 0.1M Cacodylate buffer, then vibratomed 50 um tissue slabs. I
used 5-20mg DAB(10mg is good) diluted in 0.1M Tris-HCl buffered to 9.0 pH,
add 0.02% H2O2, then incubate 30-60 (you can extend to 120) min at 37C.
Rinse in Tris buffer and post-fix in glut, osmicate, dehydrate and embedd in
epoxy resin.
This was in normal rat liver and the label was good. Make sure you
are using really fresh H2O2. Some of the older published articles have EM
pictures with very dark label, but these days you should expect less DAB
intensity. I have talked to a biochemistry/liver researcher about the
decrease in intensity of DAB label, and she was certain that the changes
were do to modern manufactured food supplements which are different from
what was fed to rats back in the 60's and 70's.
One last thing about liver peroxisomes, they are more numerous in
the centrolobular areas of the liver.

Good Luck!

} ----------
} From: Tom Januszewski[SMTP:tom.januszewski-at-email.swmed.edu]
} Reply To: tom.januszewski-at-email.swmed.edu
} Sent: Thursday, February 22, 2001 1:39 PM
} To: microscopy-at-msa.microscopy.com
} Subject: Teorell-StenhagenBuffer
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Listservers,
}
} I'm trying to track down a
} formulation for Teorell and
} Stenhagen buffer. A 2.5mM
} concentration of diaminobenzidine
} is used with this buffer and
} hydrogen peroxide to selectively
} stain the catalase in peroxisomes.
} The references that I have found
} all seem to use this buffer and
} reference an article in a German
} journal dating to 1938.
} Is anyone out there familiar with
} this buffer? If so, can you supp;y
} the recipe? Is there another buffer
} that I can use to effectively stain peroxisomes?
} As always, thanks in advance for
} your replies.
}
} Tom Januszewski
} Senior Electron Microscopist
} UT Southwestern Medical Center at Dallas
} Dallas, TX 75390-9039
} 214-648-7291
} FAX: 214-648-6408
} Email: tom.januszewski-at-UTSouthwestern.edu
}


From daemon Fri Feb 23 10:31:00 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 23 Feb 2001 11:27:35 -0500
Subject: RE: Correction on response to Teorell-StenhagenBuffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have to correct a mistake, I realized as soon as I sent the e-mail
response.......I'm sorry, I have a really bad cold and I mistakenly said
there was a change in DAB labeling intensity, which is not what I wanted to
say!!

The change is in the number of peroxisomes present in the rat liver due to
the dietary changes from the 60's to present day.




} ----------
} From: Tom Januszewski[SMTP:tom.januszewski-at-email.swmed.edu]
} Reply To: tom.januszewski-at-email.swmed.edu
} Sent: Thursday, February 22, 2001 1:39 PM
} To: microscopy-at-msa.microscopy.com
} Subject: Teorell-StenhagenBuffer
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Listservers,
}
} I'm trying to track down a
} formulation for Teorell and
} Stenhagen buffer. A 2.5mM
} concentration of diaminobenzidine
} is used with this buffer and
} hydrogen peroxide to selectively
} stain the catalase in peroxisomes.
} The references that I have found
} all seem to use this buffer and
} reference an article in a German
} journal dating to 1938.
} Is anyone out there familiar with
} this buffer? If so, can you supp;y
} the recipe? Is there another buffer
} that I can use to effectively stain peroxisomes?
} As always, thanks in advance for
} your replies.
}
} Tom Januszewski
} Senior Electron Microscopist
} UT Southwestern Medical Center at Dallas
} Dallas, TX 75390-9039
} 214-648-7291
} FAX: 214-648-6408
} Email: tom.januszewski-at-UTSouthwestern.edu
}


From daemon Fri Feb 23 10:38:15 2001



From: Xinran Liu :      xinran.liu-at-UTSouthwestern.edu
Date: Fri, 23 Feb 2001 10:28:55 -0600
Subject: Mouse brain vibratome section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, colleagues,

We have a question regarding a trouble shooting of chatter/compression on
brain sections.

The vibratome we used is VIBRATOME 3000, automated with refrigeration, the
mouse brain tissue was fixed in 4% paraformaldehyde, the thickness of
section cut was 30-50 um. We have being adjusting either speed or amplitude,
however, it doesn't seem to solve the problem.

We would appreciate anyone who would kindly help us to solve this problem.

Many thinks.

Xinran

***********************************************************
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
UT Southwestern Medical Center
6000 Harry Hines Blvd., NA4. 214A
Dallas, TX 75390-9111

Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu



From daemon Fri Feb 23 11:14:21 2001



From: pmiller-at-mbt.com
Date: Fri, 23 Feb 2001 12:11:58 -0500
Subject: statistics on quantitative microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know if there are training courses anywhere in North
America on the statistics of quantitative microscopy or stereology. If so,
where, or where can I search? Pmiller-at-mbt.com



From daemon Fri Feb 23 11:14:21 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 23 Feb 2001 08:51:46 -0800
Subject: S.S. etch

Contents Retrieved from Microscopy Listserver Archives
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Dear Bruce,
I have not done this etch myself, but my copy of Van der Voort has an etch
specifically for SEM of stainless steels:
"5 ml Acetic acid
5 ml HNO3
15 ml HCl Aqua regia plus acetic acid. For ferritic grades (of
stainless steel). Swab sample 15 s. Deep etch for SEM after 45 s."
I hope this helps.
You wrote:
Question: What solution and method would one use to deep-etch Type 316
STAINLESS STEEL to reveal the inclusions and/or precipitates present in
relief. This will be used for SEM analysis.
Thankyou in advance for your assistance.
Bruce

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Feb 23 11:43:22 2001



From: DrJohnRuss-at-aol.com
Date: Fri, 23 Feb 2001 12:39:27 EST
Subject: Re: statistics on quantitative microscopy

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 2/23/01 1:26:35 PM, pmiller-at-mbt.com-at-sparc5.microscopy.com
writes:

} I would like to know if there are training courses anywhere in North
} America on the statistics of quantitative microscopy or stereology. If
} so, where, or where can I search? Pmiller-at-mbt.com

The N. C. State Univ. course on quantitative image processing and stereology
has been taught for 20 years now. The next session is May 9-11 and there are
still some openings available. You can get full information at
http://members.aol.com/IPCourse
including syllabus, lab materials and books, on-line registration, and a
downloadable brochure, or call Cindy Allen at 919 515 8171


From daemon Fri Feb 23 11:48:52 2001



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Fri, 23 Feb 2001 10:43:24 -0700
Subject: Metallograph and Digital Camera for Light Microscopy

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Good Day Listers,

I am in the wonderful position of purchasing a new inverted metallograph
with digital image acquisition for our laboratory.

I have pretty much decided on the Nikon Epiphot 200 metallograph with
brightfield and polarized light modules. Also chosen were the Plan Achromat
objectives. Any comments on this choice?

Now the real question. Which digital camera would you buy? I know these
things have been discussed greatly here in the past and I have followed them
with great interest.

One of our primary wants is live image preview(} 15 fps).

We are leaning towards the Spot RT, although the Insight looks to be equally
promising.

Any comments for or against either of these would be appreciated. Also, if
anyone has another preference I would appreciate hearing your comments.

Thanks

William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com



From daemon Fri Feb 23 12:23:02 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 23 Feb 2001 12:14:53 -0600
Subject: 42 inch color printers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know this isn't a microscopy question but suspect many of the
microscopy core types on the listserver are responsible for the
large, poster-sized format printers. I plan to buy one and would
appreciate recommendations (both good and bad ones!). If you have
any comments on the workload to maintain and use them, that would
also be useful. TIA, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Feb 23 13:30:20 2001



From: JHoffpa464-at-aol.com
Date: Fri, 23 Feb 2001 14:25:43 EST
Subject: subscribe

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From daemon Fri Feb 23 14:24:32 2001



From: David Mathes :      dtm8p-at-virginia.edu
Date: Fri, 23 Feb 2001 15:18:14 -0500
Subject: stain

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Hi,
I am wondering if anyone knows of a staining method that might allow
one to distiguish between a region of proton implanted GaAs and an
un-implanted region in a TEM?

Also, does anyone have a URL for a microscopy web forum?

--
David Mathes
Department of Materials Science
University of Virginia
116 Engineer's Way
Charlottesville, VA 22904
804 982 5683




From daemon Fri Feb 23 14:55:33 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 23 Feb 2001 14:59:56 -0600
Subject: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
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Hi Microlisters,

Just a few quick comments on the polymerisation of LR White:

I've been using microwave oven curing of LR White for over two years now, I
and others who use this resin for gold labeling studies. The blocks section
VERY easily, silver after silver section just roll off the ol' diamond knife
edge.

Like others have reported, we also coat grids (300# nickel in these cases,
sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two
short rinses in acetone) with Formvar, then coat with evaporated carbon.
THEN use them to pick up sections. They are very stable under the beam and
we have expeienced virtually no stability problems.

Can't really say if microwave curing aids the stability of the sections or
not. We do that primarily because it saves time, and the resulting blocks
section easily.

We use the Formvar support film primarily to increase stability of sections
because of the many gold labeling solutions and rinses the sections on grids
have to go through, the carbon coating is primarily for stability under the
TEM beam.

Gib Ahlstrand




From daemon Fri Feb 23 15:24:24 2001



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Sat, 24 Feb 2001 10:20:54 +1300
Subject: Re: S.S. etch

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For deep etching of austenitic stainless steels use 10% bromine in
methanol. see E.M. Mahla and N.A. Nielsen, Carbide Precipitation in
Type 304 Stainless Steel-An Electron Microscope Study, Trans. ASM
43:290-322 (1951).


From daemon Fri Feb 23 15:29:51 2001



From: Jane LaGoy :      jlagoy-at-bodycote-imt.com
Date: Fri, 23 Feb 2001 11:23:30 -0500
Subject: re: deep etch 316 SS

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If you cannot get an answer through this microscopy forum, try
www.metallography.com "Ask the Experts"; I've found it to be very helpful.



From daemon Fri Feb 23 15:55:21 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 23 Feb 2001 14:00:30 -0500
Subject: Re: 42 inch color printers?

Contents Retrieved from Microscopy Listserver Archives
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Hi Tom...

We just got an Epson 9500 large format printer (44 inch). Works great and
was highly rated.

Best,

Angela

At 12:14 PM 02/23/2001 -0600, Tom Phillips wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Voice: (212)769-5977
Fax: (212)496-3480
---------------------------------------------


From daemon Fri Feb 23 16:21:05 2001



From: Tom W Bargar :      tbargar-at-unmc.edu
Date: Fri, 23 Feb 2001 16:17:10 -0800
Subject: TEM, immunogold protocols for Insulin, glucagon

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Anyone have protocols for immunogold labelling of insulin and glucagon in
pancreatic islet cells?

Also, many thanks to everyone who responded about the LR White stability
problem.

Tom Bargar
EM Lab, UNMC



From daemon Fri Feb 23 16:26:17 2001



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Fri, 23 Feb 2001 16:03:34 -0500
Subject: Calibration beads for fluorescence

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Has anyone used InSpeck calibration beads from Molecular Probes? I
recently purchased InSpeck green and I tried several combinations of
microscope and CCD camera settings but I can only read intensity values
from 3 concentrations, i.e. if I adjust for the highest concentration I can
only image (get readings)3 concentrations and the same is true if I start
at the lowest concentration. Also the values I obtained for the 3
concentrations is not linear. Does anyone know if there are other beads
available or how else can one calibrate the fluorescence intensity in a
fluorescence microscope equipped with a 100W HBO lamp? I would appreciate
comments or suggestions.

Thanks,

Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747


From daemon Fri Feb 23 17:09:35 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 23 Feb 2001 17:05:32 -0600
Subject: RE: Ask-A-Microscopist:deep-etch Type 316 SS

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To observe inclusions it is better do not etch steel at all, because
etching could hide them (especially deep etch). It is true for both
polished cross sections and fracture surfaces.
EDS and/or BSE detector could be helpful in the beginning when you
are learning how to distinguish inclusions from other features of
your sample.

Vladimir


} Question: What solution and method would one use to deep-etch Type 316
} STAINLESS STEEL to reveal the inclusions and/or precipitates
} present in
} relief. This will be used for SEM analysis.
} Thankyou in advance for your assistance.
} Bruce
}
} --------------------------------------------------------------
} -------------
}
}
}


From daemon Fri Feb 23 18:18:19 2001



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Fri, 23 Feb 2001 18:27:40 -0600
Subject: TEM, immunogold protocols for Insulin, glucagon

Contents Retrieved from Microscopy Listserver Archives
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Tom, I don't know your level of expertise but I'll give you a protocol that I've used for those antibodies on pancreatic cells grwon on coverslips.
the protocol is pretty standard with LRW. I use 0.05M tris saline with 1%BSA + 0.05% Tween 20 for all solutions including washes. Place grids section side down on 30 ul drops. Filter all solutions. Note: I use different variations of this protocol but this one is easy to trouble shoot.

1. 15-20 min incubation in 5 to 10 %normal serum of animal used for the secondary antibody.
2. 1- 2 hrs in primary antibody. Try various dilutions, i.e, 1:50 to 1:500. This depends on a number of factors such as background, avidity of the antibody, etc. 1:50 is always a good one to include for one hour. It gives you a feel what changes you may need the next time.
In cases where background is too high go overnight with higher dilutions in frig -at- 4C.
3. wash by placing grids on several drops of buffer, i.e. 6 drops
4. 30 -60min in 1:40, 5 or 10nm gold conjugated anti-IgG of the animal that the primary was produced in.
5. wash grids first with 5 drops of buffer followed by 5 drops of distilled water.
6. If you are going to dual label repeat the steps above but now your primary is from a different animal so your first step or blocking step is then different. Also you will used a different size gold particle conjugated secondary.
7. Counter stain with UA and lead. Contrary to what has been said I prefer staining very briefly in UA as a saturated soln in 50% ETOH followed by washing in a descending series of ETOH (50%,25% 10%) to water. I prefer the appearance of the chromatin stained with alcoholic UA. The only problems that I have is over staining if I go much beyond 5 to 10secs. In your case I would try it if you don't feel confident using alcoholic UA used 1% aqueous but stain longer like a minute
The staining depends on your preferences. The only problem if you go to long you can't see the gold. Stain with 30 to 60 secs.
I have been doing immunolabelling with LRW in everything from seeds to cells to mammary gland without too many problems. You have to be careful at all steps of the way but it works fine. I also stay away from filmed grids if I can and use 300 to 400 mesh nickel grids for many reasons especially when I know structures containing my epitope are common like what you are looking for and the descreased viewable area due to the grids bars is not a factor. LRW in our hands survives well the numerous staining steps. If you have any more questions email me.
Good luck.

Hank Adams

Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX 77030


-----Original Message-----
} From: Tom W Bargar [SMTP:tbargar-at-unmc.edu]
Sent: Friday, February 23, 2001 6:17 PM
To: Microscopy-at-sparc5.microscopy.com


Anyone have protocols for immunogold labelling of insulin and glucagon in
pancreatic islet cells?

Also, many thanks to everyone who responded about the LR White stability
problem.

Tom Bargar
EM Lab, UNMC






From daemon Fri Feb 23 20:50:27 2001



From: cenpok :      dyy-at-cenpok.net
Date: Sat, 24 Feb 2001 10:38:31 +0800
Subject: my first post

Contents Retrieved from Microscopy Listserver Archives
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dear all,
does anyone know where the microscope adapter for nikon coolpix 990 could be available?

does anyone know where can I get mannual of microscopial analysis of feedstuffs, 1992 3rd edition, the americian association of feed microscopists?

Thanks

Yours

Yiyang DOng
Beijing, CHina


From daemon Fri Feb 23 22:29:00 2001



From: cenpok :      dyy-at-cenpok.net
Date: Sat, 24 Feb 2001 12:17:02 +0800
Subject: my second post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Does anybody have a near infrared microscope? do you know which one is the best instruments of NIR microscope ?

TIA

Yours
Yiyang Dong
Beijing


From daemon Sat Feb 24 00:55:15 2001



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Sat, 24 Feb 2001 08:48:38 +0200
Subject: Sources of noise

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Hallo All!
I've noticed a bit of electronic noise starting to creep into our
pulse processing electronics, and to eliminate it have turned the gain
down a little accordingly. I have some ideas of where it may be coming
from but thought I pick the brains of the wealth of experience out
there. So, guys n' galls what gives rise to electronic noise in pulse
processing electronics. Of course, I could just look up such elimentary
stuff on some web page or other, but where's the fun in that?
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Sat Feb 24 01:55:39 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 23 Feb 2001 13:17:00 -0700
Subject: Employment

Contents Retrieved from Microscopy Listserver Archives
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Post-Doctoral Associate

Available with the Center for High Resolution Electron Microscopy at
Arizona State University. Position is sponsored by major chemical company
and primary focus of the research will be the development and application
of environmental electron microscopy to industrially relevant catalyst
systems. Areas of particular interest include the study of phase
transformations under reaction environments, in situ polymerization,
mobility and dynamic microstructural changes. Candidate will have a Ph.D.
in material science, material physics, solid-state chemistry or chemical
engineering, with extensive experience in catalyst characterization by
transmission electron microscopy. Experience in the areas of catalyst
synthesis, testing and characterization is preferred. Please submit your
resume together and the names of 3 referees to: Dr. Peter A. Crozier,
Industrial Associates Program, Center for Solid State Science, Arizona
State University, Tempe, AZ 85287-1704, Fax (480) 965-9004, email:
crozier-at-asu.edu.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Sat Feb 24 12:30:16 2001



From: Karen Pawlowski :      Karen.Pawlowski-at-worldnet.att.net
Date: Sat, 24 Feb 2001 12:17:30 -0600
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gib and all,

This thread and a conversation with a colleague here in Dallas recently
has gotten me wondering. Has anyone else come across a situation where
a formulation for a polymer works fine in one area of the country, but
you can't get it to work in another? For instance, years ago when
I was working in Minnesota, we used a soft formula for Spurs with
fairly good results yet here in Dallas, Tx., I have to use the hardest
formula to get a block that is hard enough to section and still some-
times the blocks are soft. I also remember that we always had a whole
different set of problems to deal with in Minnesota depending on whether
it was in the middle of the very dry winter or in the humid spring and
summer.

My colleague here in Dallas is trying to use a two part polymer for
casting. He has used it successfully some years ago in another part of
the country and now he is getting too soft results. I don't know about
his protocol, but I have tried adding drierite to the chamber that my
blocks are polymerized in, but it has met with limited success.

I also remember a story my old boss told me about his bringing some
already cured "epon" blocks that had already been successfully sectioned
in Minnesota with him on sabbatical. The blocks were like goo when he
tried to section them the second time in Sweden. (This was in the late
'60s, early 70's.)

Could the success that you see, Gib, and the lack of success the others
are having be related to your location and the "elements"? I have
never used a microwave for curing, so maybe that's the difference. Does
that shorten the cure time significantly?

Anybody care to comment?

Karen Pawlowski


Gib Ahlstrand wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Microlisters,
}
} Just a few quick comments on the polymerisation of LR White:
}
} I've been using microwave oven curing of LR White for over two years now, I
} and others who use this resin for gold labeling studies. The blocks section
} VERY easily, silver after silver section just roll off the ol' diamond knife
} edge.
}
} Like others have reported, we also coat grids (300# nickel in these cases,
} sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two
} short rinses in acetone) with Formvar, then coat with evaporated carbon.
} THEN use them to pick up sections. They are very stable under the beam and
} we have expeienced virtually no stability problems.
}
} Can't really say if microwave curing aids the stability of the sections or
} not. We do that primarily because it saves time, and the resulting blocks
} section easily.
}
} We use the Formvar support film primarily to increase stability of sections
} because of the many gold labeling solutions and rinses the sections on grids
} have to go through, the carbon coating is primarily for stability under the
} TEM beam.
}
} Gib Ahlstrand


From daemon Sat Feb 24 13:26:29 2001



From: Smartech :      smartech-at-javanet.com
Date: Sat, 24 Feb 2001 14:30:18 -0500
Subject: Can anyone ID these disks on a SEM micrograph of a Foam Sample?

Contents Retrieved from Microscopy Listserver Archives
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I was analyzing some foam samples and observed small disks (about 25
microns) on the cell walls on one sample. Does anyone out there know what
they are? The foam is non-ridged.

http://www.semguy.com/gfx/St400x.jpg

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Sun Feb 25 22:03:29 2001



From: aowao-at-mail-box.cz
Date: Sun, 25 Feb 2001 23:23:48 -0800
Subject: Consolidate Debt Without a Loan___

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr.Dong,

I'm Okugawa from Nikon Japan.
The adapter is available in China. Could you contact the following woman
who is representative in Beijing office.

Product: MXA29005
Contact: Ms. Wang, phone: 010-6515-5637 or 5639

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp
----- Original Message -----
} From: "cenpok" {dyy-at-cenpok.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Saturday, February 24, 2001 11:38 AM


Dear Dr.Dong,

This is Okugawa again.

What application do you like to use NIR microscope for?
How long wavelength are you interested?
Objective effects the image performance. Most of our standard
objectives transmit and make good images until 1100nm.
Many of them transmits more than 50% and some transmit
more than 70%.
I may help you technically with your requests. Please contact
the e-mail below personally.

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp
----- Original Message -----
} From: "cenpok" {dyy-at-cenpok.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Saturday, February 24, 2001 1:17 PM


How would you like to take all of your credit cards,
reduce or eliminate the interest, pay 70% less per
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From daemon Mon Feb 26 08:31:30 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Mon, 26 Feb 2001 08:28:57 -0600
Subject: Metallograph

Contents Retrieved from Microscopy Listserver Archives
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Bill Giles:

In addition to the polarized light module, I would suggest getting
the DIC (Nomarski) module. This will provide contrast to low contrast etched
microstructures such as martensite and acicular structures. We find it very
useful for low carbon steels.

Sam Purdy
Technical Center
National Steel Corp.
Trenton, MI
spurdy-at-nationalsteel.com




From daemon Mon Feb 26 08:53:46 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 26 Feb 2001 15:00:27 +0000 (GMT Standard Time)
Subject: EM support vacancies - UK

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The Department of Materials, University of Oxford, UK, has
three vacancies in its Electron Microscope Facility - one
senior Engineer and two technicians.
The details can be found at
http://www.materials.ox.ac.uk/vacancies/vacancieshome.html#SupportStaff.

Closing date for applications is 23rd March 2001.

Please bring this to the attention of anyone who may be
interested.

Thanks
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Mon Feb 26 09:27:02 2001



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Mon, 26 Feb 2001 10:23:34 -0500
Subject: Quartz Crystals

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I am looking for micro-crystalline quartz crystals, up to 100
microns in size. Does anybody know of a source? Thank you, Peggy.
--


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com






From daemon Mon Feb 26 10:33:48 2001



From: PMarcum :      pmarcum-at-p3.net
Date: Mon, 26 Feb 2001 11:25:25 -0500
Subject: Re: TEM, stability problems of LR White sections in EM beam

Contents Retrieved from Microscopy Listserver Archives
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Dear Gib and All,
We get calls from all over the country and the increase in technical calls
with problems is different for some areas. The time of year and weather
conditions can and do play a part in how somethings react and polymerize.
It is difficult to tell when or where this will happen and often we
recommend drying with dissicant at one point and adding moisture for others.
This sounds a little strange and as if the question is being avoided
however, you bring up a good point and the changes in the environment should
be considered when getting a protocol from a colleague in another area or
country. It has caused people to become very angry when they think
something is left out or not discussed. in actuality the problem can be the
change in environment or even pH of the distilled, DI, or tap water.
Histology has these problems often with season changes and the addition of
heat or cooling of the laboratory air. Pam Marcum

-----Original Message-----
} From: Karen Pawlowski [mailto:Karen.Pawlowski-at-worldnet.att.net]
Sent: Saturday, February 24, 2001 1:18 PM
To: Gib Ahlstrand
Cc: Microscopy-at-sparc5.microscopy.com


Hi Gib and all,

This thread and a conversation with a colleague here in Dallas recently
has gotten me wondering. Has anyone else come across a situation where
a formulation for a polymer works fine in one area of the country, but
you can't get it to work in another? For instance, years ago when
I was working in Minnesota, we used a soft formula for Spurs with
fairly good results yet here in Dallas, Tx., I have to use the hardest
formula to get a block that is hard enough to section and still some-
times the blocks are soft. I also remember that we always had a whole
different set of problems to deal with in Minnesota depending on whether
it was in the middle of the very dry winter or in the humid spring and
summer.

My colleague here in Dallas is trying to use a two part polymer for
casting. He has used it successfully some years ago in another part of
the country and now he is getting too soft results. I don't know about
his protocol, but I have tried adding drierite to the chamber that my
blocks are polymerized in, but it has met with limited success.

I also remember a story my old boss told me about his bringing some
already cured "epon" blocks that had already been successfully sectioned
in Minnesota with him on sabbatical. The blocks were like goo when he
tried to section them the second time in Sweden. (This was in the late
'60s, early 70's.)

Could the success that you see, Gib, and the lack of success the others
are having be related to your location and the "elements"? I have
never used a microwave for curing, so maybe that's the difference. Does
that shorten the cure time significantly?

Anybody care to comment?

Karen Pawlowski


Gib Ahlstrand wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Microlisters,
}
} Just a few quick comments on the polymerisation of LR White:
}
} I've been using microwave oven curing of LR White for over two years now,
I
} and others who use this resin for gold labeling studies. The blocks
section
} VERY easily, silver after silver section just roll off the ol' diamond
knife
} edge.
}
} Like others have reported, we also coat grids (300# nickel in these cases,
} sonicated before use to clean in 10% acetone, 10% HCl, 80% DW, plus two
} short rinses in acetone) with Formvar, then coat with evaporated carbon.
} THEN use them to pick up sections. They are very stable under the beam and
} we have expeienced virtually no stability problems.
}
} Can't really say if microwave curing aids the stability of the sections or
} not. We do that primarily because it saves time, and the resulting blocks
} section easily.
}
} We use the Formvar support film primarily to increase stability of
sections
} because of the many gold labeling solutions and rinses the sections on
grids
} have to go through, the carbon coating is primarily for stability under
the
} TEM beam.
}
} Gib Ahlstrand



From daemon Mon Feb 26 11:03:24 2001



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Mon, 26 Feb 2001 09:57:34 -0700
Subject: RE: Metallograph and Digital Camera for Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Lawrence,

Thanks for your response.

I read the literature available on the indicated web site and have a couple
of questions.

There seems to be some conflicting information.
Under specifications:
Image size 3840 x 3072
CCD 1.34 M pixels
Document text: 12 million pixels (3046 x 3072) ???????

Is my math wrong or am I missing some pertinent information?

How do you obtain RGB? What does the color array on the chip look like? Or
does the camera use filters?

What's the s/n ratio?

Pixel size?

Are there twain drivers available or a Photoshop plugin?

Is any annotaion, image enhancement and/or image analysis software included?

BTW, the Spot RT is $7k also, not twice that price.

William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com

} -----Original Message-----
} From: Lawrence Kordon [SMTP:nikon-at-jagunet.com]
} Sent: Friday, February 23, 2001 1:38 PM
} To: Giles, Bill
} Cc: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Re: Metallograph and Digital Camera for Light Microscopy
}
} William,
}
} Excellent choice on the Nikon Epiphot 200! However, why are you not
} considering
} the Nikon DXM1200 Digital Camera? It is a whopping 12 Million pixels with
} a live
} Video Output to a PC and cost under $7000. That is about the half the
} price of
} the Spot-RT. Also, why would you want to have a cooled CCD anyway?
} Metallurgy;
} even with Fluorescence and high Mag DIC (Pol) does not require it. Not to
} mention this camera can do extremely low light anyway! This Camera is
} literally
} the hottest product in Microscopy today! We have sold hundreds already
} "sight/unseen" and are first shipping them. All the major Image Analysis
} companies have or are writing Drivers and it is so popular we have put it
} on
} other brands microscopes as well! By the way, the US MINT and some NIST
} Labs
} here is Washington, DC have it. Please check out the specs at....
} http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM
} 1200
} and call you local Nikon Dealer for a Demonstration and Quotation.
}
} Good Luck,
}
} Lawrence Kordon
} Nikon Instruments, Inc.
} Columbia, MD
} nikon-at-jagunet.com
}
}
}
} "Giles, Bill" wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Good Day Listers,
} }
} } I am in the wonderful position of purchasing a new inverted metallograph
} } with digital image acquisition for our laboratory.
} }
} } I have pretty much decided on the Nikon Epiphot 200 metallograph with
} } brightfield and polarized light modules. Also chosen were the Plan
} Achromat
} } objectives. Any comments on this choice?
} }
} } Now the real question. Which digital camera would you buy? I know these
} } things have been discussed greatly here in the past and I have followed
} them
} } with great interest.
} }
} } One of our primary wants is live image preview(} 15 fps).
} }
} } We are leaning towards the Spot RT, although the Insight looks to be
} equally
} } promising.
} }
} } Any comments for or against either of these would be appreciated. Also,
} if
} } anyone has another preference I would appreciate hearing your comments.
} }
} } Thanks
} }
} } William T. Giles
} } Sr. Electron Microscopist
} } Met. Lab. Coordinator
} } Henderson Technical Laboratory
} } TIMET
} } PO Box 2128 Henderson NV 89009
} } Ph: (702)566-4436
} } Fax: (702)564-9038
} } E-mail: Bill.Giles-at-timet.com


From daemon Mon Feb 26 11:09:33 2001



From: Edward J. King :      king-at-biology.utah.edu
Date: Mon, 26 Feb 2001 10:14:56 -0700
Subject: Electron Microscopy Technician opening

Contents Retrieved from Microscopy Listserver Archives
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I've been asked to distribute the following announcement of an available
position for a TEM technician at the University of Utah. Anyone who's
interested should contact Dr. Kendal Broadie.

Ed King
____________________________

Electron Microscopy Lab Technician

The ideal candidate will have a bachelor degree in a biological science and
experience in electron microscopy. Exposusure to neuroscience and/or
genetics would be a benefit. The job includes preparing specimens for
microscopy, operation of electron microscopes, producing photographic and
digital micrographs and analyzing ultrastructural data. Training will be
provided.
If interested, please submit an application at the University of Utah Human
Resources. If you would like more information about job specifics, please
contact me (Kendal Broadie) at e-mail: broadie-at-biology.utah.edu

Dr. Kendal S. Broadie (801) 585-9426 (office; 448 Skaggs)
Department of Biology (801) 585-9425 (main lab; 450 Skaggs)
University of Utah (801) 585-1301 (lab II; 460 Skaggs)
257 South 1400 East (801) 581-4668 (fax)
Salt Lake City, Utah 84112-0840 broadie-at-biology.utah.edu

lab web page: http://synapse.biology.utah.edu




From daemon Mon Feb 26 12:18:56 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 26 Feb 2001 13:13:07 -0500
Subject: Re: Quartz Crystals

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I am looking for micro-crystalline quartz crystals, up to 100
microns in size. Does anybody know of a source? Thank you, Peggy.
--

Dear Peggy,
I have seen some small quarz crystals while examining river sediment.
They're about 1 micron in size, EDS shows lots of Si, and no Na, Al, K, Ca, Fe
or Ti--the most common other elements in silicates. I'm also not sure how best
to separate them from the other material, although I would think that floating
the sediment on an appropriate liquid would work. The Handbook of Chemistry and
Physics has a table of Heavy Liquids for Mineral Separation on page E-376 (62nd
Edition). Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Mon Feb 26 12:33:52 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 26 Feb 2001 12:28:43 -0600
Subject: 2 post-doc positions and/or Sr. Tech position

Contents Retrieved from Microscopy Listserver Archives
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Two newly funded positions are available to study intracellular
protein trafficking using LM and EM immunocytochemistry. Ideally,
these positions will be filled by either two postdoctoral fellows or
one postdoc and one senior research technician. The projects use
similar approaches but are funded by separate sources.

The first project involves using confocal microscopy and electron
microscopy, to visualize lipoproteins, lipid synthesizing enzymes,
and components of the ubiquitin-proteasome system in hepatic and
intestinal cells. Wild-type and heterologous cells will be used to
map the location of these proteins in cell compartments and the
secretory pathway. Live cell recording using transgenic cells with
GFP-labeled constructs are part of this study.

The second project examines the intracellular trafficking and storage
of transgenic proteins in maize and other plant seed tissues. The
ideal candidate would have experience in plant microscopy and/or
light and electron microscopic immunocytochemistry.

Individuals with experience in electron microscopy and prior training
in ultramicrotomy are especially encouraged to apply for either
position.

Please send a curriculum vitae, cover letter and the names of three
references to Dr. Tom Phillips, Division of Biological Sciences, 3
Tucker Hall, University of Missouri, Columbia, MO 65211 7400.
Enquires can also be directed to PhillipsT-at-missouri.edu.



--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Mon Feb 26 13:08:28 2001



From: Steve Widing :      swiding-at-astro.ocis.temple.edu
Date: Mon, 26 Feb 2001 14:04:36 -0500 (EST)
Subject: Huxley microtome

Contents Retrieved from Microscopy Listserver Archives
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Hello List,

Does anyone have a contact or supplier for parts for a Huxley
Ultra Microtome MK2?

Thanks,

Steve Widing
Temple University




From daemon Mon Feb 26 13:14:57 2001



From: George Falb :      gfalb-at-buckeyenutrition.com
Date: Mon, 26 Feb 2001 14:06:48 -0500
Subject: my first post

Contents Retrieved from Microscopy Listserver Archives
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Yiyang DOng...

For your Nikon adapter I would suggest you contact McCrone Microscopes &
Accessories from Westmont, IL 60559. They can be contacted on the web at
www.mccrone.com or e-mail address info-at-mccrone.com
or phone 630-887-7100 or Fax 630-887-7764. If they don't
have it they should be able to direct you to who can.

As far as the 1992 Microscopy Analysis of Feedstuffs you can contact the
American Oilseed Chemist Society at www.aocs.org. Please contact them and
they will be happy to help.

George Falb
AOCS/Feed Microscopy Division Chair

----- Original Message -----
} From: "cenpok" {dyy-at-cenpok.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 23, 2001 9:38 PM


dear all,
does anyone know where the microscope adapter for nikon coolpix 990 could be
available?

does anyone know where can I get mannual of microscopial analysis of
feedstuffs, 1992 3rd edition, the americian association of feed
microscopists?

Thanks

Yours

Yiyang DOng
Beijing, CHina








From daemon Mon Feb 26 13:38:07 2001



From: Laura Lopez :      laural-at-ccmc.unam.mx
Date: Mon, 26 Feb 2001 11:05:34 -0600
Subject: BSE detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello all,

I'm doing my master in materials science and recently a BSE detector was

installed in the SEM. I'm working with it and I want to know how the
detector
physically works. The manual just said that the detector is made of
semiconducor material. The detector is a GW Electronics system 47 solid
state detector. Could some body help me with that description ??


Laura Lopez
CCMC-UNAM
Ensenada, B.C. Mexico



From daemon Mon Feb 26 14:02:45 2001



From: Kelly Randall :      kellyr-at-neuro.hfh.edu
Date: Mon, 26 Feb 2001 15:01:13 -0500 (EST)
Subject: Re: Mouse brain vibratome section

Contents Retrieved from Microscopy Listserver Archives
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Xinran,
I have not had too much experience in vibratoming mouse brains, but I do
vibratome rat brains on a regular basis. I have only had problems when
the tissue has not been fixed long enough, or when I am cutting 25-30 um
sections. Usually turning down the amplitude to a very low setting has
worked. Sorry that I couldn't be much more help.
Contact me if you have any other specific questions.
KElly Randall
Henry Ford Hospital
kellyr-at-neuro.hfh.edu


On Fri, 23 Feb 2001, Xinran Liu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, colleagues,
}
} We have a question regarding a trouble shooting of chatter/compression on
} brain sections.
}
} The vibratome we used is VIBRATOME 3000, automated with refrigeration, the
} mouse brain tissue was fixed in 4% paraformaldehyde, the thickness of
} section cut was 30-50 um. We have being adjusting either speed or amplitude,
} however, it doesn't seem to solve the problem.
}
} We would appreciate anyone who would kindly help us to solve this problem.
}
} Many thinks.
}
} Xinran
}
} ***********************************************************
} Xinran Liu, M.D., Ph.D.
} Center for Basic Neuroscience
} UT Southwestern Medical Center
} 6000 Harry Hines Blvd., NA4. 214A
} Dallas, TX 75390-9111
}
} Phone: (214) 648-1830
} Fax: (214) 648-1801
} E-mail: Xinran.Liu-at-UTsouthwestern.edu
}
}
}


From daemon Mon Feb 26 14:06:12 2001



From: HARGER-ALLEN.MARGARET_J-at-INDIANAPOLIS.VA.GOV
Date: 26 Feb 2001 15:06:20 -0500 (EST)
Subject: TEM processing/formalin fixed tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
microscopy-at-sparc5.microscopy.com



From: HARGER-ALLEN.MARGARET_J-at-INDIANAPOLIS.VA.GOV
Date: 26 Feb 2001 15:06:20 -0500 (EST)
Subject: TEM processing/formalin fixed tissue

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Pathology lab. listers: are there any new/and/or superior techniques
for processing previously formalin fixed tissue for TEM?
Thanks, Peggy Harger-Allen
|TAB|email:harger-allen-at-indianapolis.va.gov


From daemon Mon Feb 26 15:57:57 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 26 Feb 2001 13:52:56 -0800
Subject: RE: Metallograph and Digital Camera for Light Microscopy

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The Nikon MX1200 is native 1.2M pixels. It, like current
generation digital microscope cameras, do a "mechanical"
interpolation to increase final pixel count. One way is
to physically move the CCD imager. Each movement
results in new image data for that position. This is a bad
way to gather more pixels, however. The other way is to
keep the CCD fixed but scan the image across it multiple
times at different angles.

Zeiss has tried to do mechanical shifting and not done
all that good of a job. Looks like Nikon is trying it too.
They are up against a basic patent by Pixera. Pixera
uses what they call a "Diractor" to scan the image
across a fixed CCD.

http://www.pixera.com/PixeraCatalog/Penguin/Penguin.htm

The Pixera is available as a 1.5M pixel unit or as a 5.8M pixel
Diractor unit. Each are also available with Peltier cooling.
Like the Nikon, the Pixera uses a dedicated PCI card for
PC or Mac. This card, camera and software will do
live focus at 12-15 fps on a 733MHz system. It does
manual and automatic exposure and also provides a focus
indication bar. Also provided is automatic or manual
white balance, selectable black balance and multi-size
spot exposure metering.

The best way to find out how well a camera performs is
to take a high resolution shot at different lighting extremes
and do an FFT on the images. Artifacts and garbage will
show up if the system produces high pixel count at the
expense of image quality.

I use a Pixera Penguin 600CL and like it very much. It
is easy to take single shots either as one exposure or
averaged or integrated up to 256 exposures. Averaging
and Peltier cooling greatly reduces ultimate noise in the
image. For simple documentation purposes, this is
not necessary. But for images which will undergo
further processing, noise and artifacts must be reduced
or eliminated. The Pixera has selectable ISO from 50 to 400.
I run it at ISO 100. No info on what the Nikon does.

The Pixera also supports Twain interfacing.

gary g.

At 08:57 AM 2/26/01, you wrote:

} Lawrence,
}
} Thanks for your response.
}
} I read the literature available on the indicated web site and have a couple
} of questions.
}
} There seems to be some conflicting information.
} Under specifications:
} Image size 3840 x 3072
} CCD 1.34 M pixels
} Document text: 12 million pixels (3046 x 3072) ???????
}
} Is my math wrong or am I missing some pertinent information?
}
} How do you obtain RGB? What does the color array on the chip look like? Or
} does the camera use filters?
}
} What's the s/n ratio?
}
} Pixel size?
}
} Are there twain drivers available or a Photoshop plugin?
}
} Is any annotaion, image enhancement and/or image analysis software included?
}
} BTW, the Spot RT is $7k also, not twice that price.
}
} William T. Giles
} Sr. Electron Microscopist
} Met. Lab. Coordinator
} Henderson Technical Laboratory
} TIMET
} PO Box 2128 Henderson NV 89009
} Ph: (702)566-4436
} Fax: (702)564-9038
} E-mail: Bill.Giles-at-timet.com
}
} } -----Original Message-----
} } From: Lawrence Kordon [SMTP:nikon-at-jagunet.com]
} } Sent: Friday, February 23, 2001 1:38 PM
} } To: Giles, Bill
} } Cc: 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: Re: Metallograph and Digital Camera for Light Microscopy
} }
} } William,
} }
} } Excellent choice on the Nikon Epiphot 200! However, why are you not
} } considering
} } the Nikon DXM1200 Digital Camera? It is a whopping 12 Million pixels with
} } a live
} } Video Output to a PC and cost under $7000. That is about the half the
} } price of
} } the Spot-RT. Also, why would you want to have a cooled CCD anyway?
} } Metallurgy;
} } even with Fluorescence and high Mag DIC (Pol) does not require it. Not to
} } mention this camera can do extremely low light anyway! This Camera is
} } literally
} } the hottest product in Microscopy today! We have sold hundreds already
} } "sight/unseen" and are first shipping them. All the major Image Analysis
} } companies have or are writing Drivers and it is so popular we have put it
} } on
} } other brands microscopes as well! By the way, the US MINT and some NIST
} } Labs
} } here is Washington, DC have it. Please check out the specs at....
} } http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM
} } 1200
} } and call you local Nikon Dealer for a Demonstration and Quotation.
} }
} } Good Luck,
} }
} } Lawrence Kordon
} } Nikon Instruments, Inc.
} } Columbia, MD
} } nikon-at-jagunet.com
} }
} }
} }
} } "Giles, Bill" wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Good Day Listers,
} } }
} } } I am in the wonderful position of purchasing a new inverted metallograph
} } } with digital image acquisition for our laboratory.
} } }
} } } I have pretty much decided on the Nikon Epiphot 200 metallograph with
} } } brightfield and polarized light modules. Also chosen were the Plan
} } Achromat
} } } objectives. Any comments on this choice?
} } }
} } } Now the real question. Which digital camera would you buy? I know these
} } } things have been discussed greatly here in the past and I have followed
} } them
} } } with great interest.
} } }
} } } One of our primary wants is live image preview(} 15 fps).
} } }
} } } We are leaning towards the Spot RT, although the Insight looks to be
} } equally
} } } promising.
} } }
} } } Any comments for or against either of these would be appreciated. Also,
} } if
} } } anyone has another preference I would appreciate hearing your comments.
} } }
} } } Thanks
} } }
} } } William T. Giles
} } } Sr. Electron Microscopist
} } } Met. Lab. Coordinator
} } } Henderson Technical Laboratory
} } } TIMET
} } } PO Box 2128 Henderson NV 89009
} } } Ph: (702)566-4436
} } } Fax: (702)564-9038
} } } E-mail: Bill.Giles-at-timet.com



From daemon Mon Feb 26 15:59:47 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 26 Feb 2001 15:49:12 -0600
Subject: Re: BSE detector

Contents Retrieved from Microscopy Listserver Archives
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I am going off my recollection, which can sometimes be a dangerous thing,
but it should be a start and may be adequate.

I believe your GW BSE detector is very similar in principle to solar
voltaic cells. Incident photons create electron-hole pairs which generate a
current/voltage. You will find that such detectors are swamped when the SEM
chamber light is turned on or the chamber is opened to room light.

In the other camp are Robinson style BSE detectors. These are better
considered as scintillator type detectors. Incident electrons generate a
pulse of light which is directed down a light pipe to a photo-multiplier
tube. This kind can generate a signal suitable for TV-rate imaging, but may
not be very sensitive to low signal levels. The former kind may be more
sensitive, but are slower responding and suitable primarily for slow scans.

The most basic of SEM books should describe the difference between
backscattered and secondary electrons. The intensity of the BSE signal is
directly related to the atomic number of the phase under the beam.
Therefore, the BSE signal gives indications of compositional variation even
without EDX. I also remember seeing some articles that talked of doing
spectroscopy on the BSE signal (i.e., measuring energy of the scattered
electrons) and relating that to the material doing the scattering. However,
basic BSE systems are not setup for such measurements.

Hope this gets you started.

At 11:05 AM 2/26/2001 -0600, you wrote:

} Hello all,
}
} I'm doing my master in materials science and recently a BSE detector was
}
} installed in the SEM. I'm working with it and I want to know how the
} detector
} physically works. The manual just said that the detector is made of
} semiconducor material. The detector is a GW Electronics system 47 solid
} state detector. Could some body help me with that description ??
}
}
} Laura Lopez
} CCMC-UNAM
} Ensenada, B.C. Mexico

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Feb 26 16:44:07 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 26 Feb 2001 17:40:53 -0500
Subject: Re: BSE detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Laura,
The BSE detector is basically a solar cell and is able to generate a
current from the energy released by backscattered eleectrons impinging
on its surface. It is fairly fragile, so don't run a specimen up into
it. Also GW has done a lot of work on this and a regular solar cell
won't work because it has too much capacitance. These are very thin. I
also seem to remember that they went to a cell that was doped opposite
to the doping normally found in solar cells (P instead of N or vice
versa). The signal is then run through a preamplifier and on to the
main amplifier. For TV rates (if that was set up) I believe they
reverse bias the cell to further reduce capacitance and increase gain
(sensitivity). Secondary electrons don't have nearly enough energy to
cause the cell to react.

Ken Converse
owner
Quality Images
Delta, PA

Laura Lopez wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hello all,
}
} I'm doing my master in materials science and recently a BSE detector was
}
} installed in the SEM. I'm working with it and I want to know how the
} detector
} physically works. The manual just said that the detector is made of
} semiconducor material. The detector is a GW Electronics system 47 solid
} state detector. Could some body help me with that description ??
}
}
} Laura Lopez
} CCMC-UNAM
} Ensenada, B.C. Mexico
}
}
}
}



From daemon Mon Feb 26 16:51:33 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Tue, 27 Feb 2001 08:49:17 +1000
Subject: Re: Huxley microtome

Contents Retrieved from Microscopy Listserver Archives
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There VERY OLD, however they were manufactured by the Cambridge
Instrument Company, now part of LEO. (Cambribge SEMs). They may be able
to help you.
At one stage the Huxley Mk2 were distributed by LKB, now part of the
Leica Instruments Company, they also may be able to help you.
Regards
JVN

Steve Widing wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello List,
}
} Does anyone have a contact or supplier for parts for a Huxley
} Ultra Microtome MK2?
}
} Thanks,
}
} Steve Widing
} Temple University

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Mon Feb 26 17:59:51 2001



From: Ronald Austin :      rla-at-mindspring.com
Date: Mon, 26 Feb 2001 17:55:06 -0600
Subject: TEM processing/formalin fixed tissue

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Ronald Austin [mailto:rla-at-mindspring.com]
Sent: Monday, February 26, 2001 4:39 PM
To: "HARGER-ALLEN.MARGARET_J-at-INDIANAPOLIS.VA.GOV"-at-sparc5.microscopy.com


Pathology lab. listers: are there any new/and/or superior techniques
for processing previously formalin fixed tissue for TEM?
Thanks, Peggy Harger-Allen
|TAB|email:harger-allen-at-indianapolis.va.gov




From daemon Mon Feb 26 18:08:12 2001



From: rad0 :      rden25-at-mindspring.com
Date: Mon, 26 Feb 2001 18:06:11 -0600
Subject: how do you look at a fungus using a SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Forgive me, I haven't tried to find this on my own yet...

But have any of you used an electron microscope to get a
close-up of a fungus? Can this be done?

Thanks...



From daemon Mon Feb 26 18:23:58 2001



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Mon, 26 Feb 2001 18:15:57 -0600
Subject: LM: Need a filter cube holder for Nikon Diaphot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was wondering if anyone might have an extra filter cube holder laying
around for an older Nikon Diaphot inverted microscope.

Any help would be greatly appreciated.

Thanks,

Raj

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
**********************************************
"Innovative Tools to Explore the Microworld"


From daemon Mon Feb 26 19:01:35 2001



From: risports-at-aol.com ()
Date: Mon, 26 Feb 2001 19:01:08 -0600
Subject: Ask-A-Microscopist: American Optical Model 150 microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor
---------------------------------------------------------------------------

Email: risports-at-aol.com
Name: Keenan Kelly
School: Veterans Memorial HS

Question: I recently bought an American Optical Model 150 microscope used
on eBay and would like to add phase contrast to it.

Question 1.) Why can't I find a web site or other company information
about American Optical on the web?


Question 2.) Are objectives and condensers interchangeable between
microscopes from different manufacturers? If not all, then how do you find
out which are?



---------------------------------------------------------------------------




From daemon Mon Feb 26 19:01:35 2001



From: wastedmind-at-email.com ()
Date: Mon, 26 Feb 2001 18:59:57 -0600
Subject: Ask-A-Microscopist: Abbe's theory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor


---------------------------------------------------------------------------

Email: wastedmind-at-email.com
Name: Nick Short
School: Coupeville High


Question: Could you please explain Ernst Abbe's theory of image formation
in terms easy to understand for someone not well versed in science?

---------------------------------------------------------------------------




From daemon Mon Feb 26 19:02:16 2001



From: maegan-at-ualr.edu ()
Date: Mon, 26 Feb 2001 19:01:53 -0600
Subject: dispersion staining techniques

Contents Retrieved from Microscopy Listserver Archives
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Email: maegan-at-ualr.edu
Name: Marilyn Egan
School: University of Arkansas at Little Rock

Question: Are you familure with dispersion staining techniques? I need
information regarding microscope set up and grain orientation with respect
to the annular and central stops.

Thank you for your time.

Sincerely,
Marilyn Egan

---------------------------------------------------------------------------




From daemon Mon Feb 26 20:32:21 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 26 Feb 2001 21:31:10 -0500
Subject: Re: how do you look at a fungus using a SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

Fix in glutaraldehyde, dehydrate through a gradient ethanol and acetone
series, critical point dry, glue to a stub and sputter-coat or --attach to
a specimen mount for cryo-sem, freeze, etch by raising the temperature to
-100C, sputter-coat and image.

Rosemary



From daemon Tue Feb 27 03:43:05 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Tue, 27 Feb 2001 10:21:58 +0100
Subject: Tripod Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I am looking for a nice glue to fix the specimen to the tripod polisher for
wedge polishing. The "super glue" stuff does not work well. The specimen falls
off too easily.

I would appreciate very much if anyone could send me some good idea.

Best Regards
Csaba Cserhati


From daemon Tue Feb 27 03:58:31 2001



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Tue, 27 Feb 2001 10:56:20 +0100
Subject: SEM - CPD

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,

This week, our CPD was broken. I was planning to CPD some samples that
day. The samples are now in acetone. My question : how long can these
samples stay in acetone ? The samples are epithelial cells from duodenum
grown on glass.

greetings,

De Pauw Bart
Belgium



From daemon Tue Feb 27 04:21:19 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Tue, 27 Feb 2001 21:39:29 +1000
Subject: RE: SEM - CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nikon DXM1200 has 1.3M pixels CCD. With 3 x 3 times "Diractor",
it makes 12M pixels images. Because of the color array, 2 pixels should
be moved totally. DXM1200 moves two times every two thirds pixels. Pixera
moves 1 time every one pixel or three times every half pixels.
Please visit the following web to get more information

http://www.microscopyu.com/

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp
----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Giles, Bill" {William.Giles-at-TIMET.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 27, 2001 6:52 AM


Dear Sam,

DXM1200 is high sensitive enough to get good DIC images
or some fluorescence images.

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp
----- Original Message -----
} From: "Purdy, Sam" {SPurdy-at-nationalsteel.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, February 26, 2001 11:28 PM


If those cells were for TEM sections, I would be very concerned and re-hydrate
them back to 70% alcohol immediately. For SEM lipid extractions matters less
but you may well get some shrinkage. Its less urgent, but I would take those
cells back to 70%. At that and refrigerated you SHOULD see no real change for
some weeks.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw
[SMTP:Bart.DePauw-at-rug.ac.be] wrote:
}
} Hello Everyone,
}
} This week, our CPD was broken. I was planning to CPD some samples that
} day. The samples are now in acetone. My question : how long can these
} samples stay in acetone ? The samples are epithelial cells from duodenum
} grown on glass.
}
} greetings,
}
} De Pauw Bart
} Belgium
}



From daemon Tue Feb 27 08:42:46 2001



From: stacey andringa :      andrina-at-email.uc.edu
Date: Tue, 27 Feb 2001 09:37:35 -0500
Subject: fixation of mouse eyes

Contents Retrieved from Microscopy Listserver Archives
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I have been given mouse eyes in 2% paraformaldehyde/2.5% glutaraldehyde
buffered with Millonig's phosphate buffer. I was asked to rate the lens
for opacity and then to fix the eyes in tact and section them.
Any suggestions would be appreciated.
Thanks.

Stacey Andringa
andrina-at-email.uc.edu



From daemon Tue Feb 27 09:25:50 2001



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Tue, 27 Feb 2001 15:22:35 -0000
Subject: microtomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html} {DIV} Hi all, {/DIV}
{DIV} I'm from the university of Massachusetts, Lowell. We are looking for a microtome to replace the old one we have. does anyone have a old microtome they want to sell off or just trying to get rid of? {/DIV}
{DIV} Praveena {/DIV}
{DIV} Department of Chemical Engineering {/DIV}
{DIV} Unversity of Massachusetts, Lowell {/DIV}
{DIV} Lowell , MA {/DIV}
{DIV} PH: 978-934-3411 {/DIV}
{DIV}   {/DIV} {br clear=all} {hr} Get Your Private, Free E-mail from MSN Hotmail at {a href="http://www.hotmail.com"} http://www.hotmail.com {/a} . {br} {/p} {/html}


From daemon Tue Feb 27 11:07:36 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 27 Feb 2001 09:00:44 -0800
Subject: Re: how do you look at a fungus using a SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear rad0,
I have looked at fungus and fungus spores on my SEM and they are very simple
to prepare. Just stick them to a stub by sticky tab or glue and then gold
coat. They are very robust and they don't need any fixing or dehydration.
At 06:06 PM 2/26/01 -0600, you wrote:
} Hello,
}
} Forgive me, I haven't tried to find this on my own yet...
}
} But have any of you used an electron microscope to get a
} close-up of a fungus? Can this be done?
}
} Thanks...
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Feb 27 12:54:55 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 27 Feb 2001 10:56:36 -0800
Subject: Re: how do you look at a fungus using a SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rad0

You might try exposing your non-aquatic sample (bread molds, mushrooms,
etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4%
OsO4 on the lid of a very small container with your sample inside or the
lid over the sample directly), then air dry, mount, coat and view. As with
any sample, the drying artifacts vary with the fungus. Some things look
great.

good luck

Steve
}
} Hello,
}
} Forgive me, I haven't tried to find this on my own yet...
}
} But have any of you used an electron microscope to get a
} close-up of a fungus? Can this be done?
}
} Thanks...



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/





From daemon Tue Feb 27 15:20:54 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 27 Feb 2001 16:00:55 -0500
Subject: RE: Tripod Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There has been a lot of discussion over the past on this subject. The boys from IBM are the experts. (There's your plug, Ron.) Since nobody has answered this yet, I'll give you my summary. Ron, If I say anything wrong, please correct me.

Don't use any type of super glue gel! It doesn't work, you have to use the thin stuff.

The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes difficult to locate in other parts of the country.

I have had good success with the Alpha superglue from Electron Microscopy Sciences and with Loctite superglue available almost anywhere. Buy it from a place that has a high turnover rate of it so that it is fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator for any length of time. Once it has been opened, you can't use it for very long (a day or two). I have used the Loctite a little longer because it has a very good sealing top.

I think that pressure on the sample while it cures is important, but the IBM guys just wick the extra stuff away from the sample and don't put a lot of pressure on the sample.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Csaba Cserhati [mailto:cserhati-at-delfin.klte.hu]
Sent: Tuesday, February 27, 2001 4:22 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Tripod Polisher


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---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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---------------------------------------------------------------
--------.


Dear Microscopists,

I am looking for a nice glue to fix the specimen to the tripod
polisher for
wedge polishing. The "super glue" stuff does not work well.
The specimen falls
off too easily.

I would appreciate very much if anyone could send me some good idea.

Best Regards
Csaba Cserhati



From daemon Tue Feb 27 15:42:38 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 27 Feb 2001 16:45:04 -0500
Subject: Re: fixation of mouse eyes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 9:37 AM -0500 2/27/01, stacey andringa wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

***********************
Hi Stacey,

If you must leave the eyes intact, I would suggest REALLY prolonging
everything: at least 30 minutes in each alcohol, and take about a
day & a half for the infiltration. Use a rotator during all steps.
I've had better luck using Epon-Araldite for eyes, although I've also
used Spurr's.

If its at all possible, find out if you (or the person you are doing
this for) can make a few small holes through the sclera to give
better access of solutions to the internal structures. eyes are very
complex, with lots of layers of different cell types and different
consistencies, so you really need to get your reagents to penetrate
thoroughly. The vitreous, in the center, is frequently problematic
and one of the main reasons for needing the long steps.

Eyes are not easy, but when you've don a good job, they are
beautiful. Stain your 1 micron sections with methylene
blue...gorgeous!

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Feb 27 15:55:23 2001



From: Bob Bagnell :      rml-at-grayhawk.med.unc.edu
Date: Tue, 27 Feb 2001 16:52:26 -0400
Subject: Phase Contrast Artifacts

Contents Retrieved from Microscopy Listserver Archives
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What are some good demonstration specimens that clearly show phase halo and
shading off? I am especially interested in demonstrating shading off.

Bob

_____________________________________________________________
C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof.
Microscopy Services Laboratory
Department of Pathology & Laboratory Medicine
CB #7525 UNC-CH, Chapel Hill, N.C. 27599
ph 919-966-2413 fx 919-966-6718
http://www.pathology.med.unc.edu/path/microscopy/welcome.htm




From daemon Tue Feb 27 16:33:02 2001



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Tue, 27 Feb 2001 15:26:24 -0700
Subject: Metallograph and Digital Camera for Light Microscopy- Follow up

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all those Listers that responded both on and off line(RH).
We've decided to stick with our original decisions.

Any end users or potential purchasers should contact me off line, I'd be
more than happy to share my learning experiences(good and bad).

Anyone needing titanium metallography or application assistance, give us a
call!

Once again thanks to Nestor for providing this forum for open exchange.

William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com



From daemon Tue Feb 27 16:37:08 2001



From: Thurberg, Beth :      Beth.Thurberg-at-genzyme.com
Date: Tue, 27 Feb 2001 17:31:53 -0500
Subject: Confocal Microscopist position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


CONFOCAL MICROSCOPIST POSITION OPEN AT GENZYME CORPORATION (FRAMINGHAM, MA)

Position: Scientist- Staff I

Description of Duties:

This individual will be in charge of Genzyme's confocal microscopy
facility, and will interact with multiple research groups across the Company
who utilize this technology. The individual will also be a key player in
research efforts within the Pathology department, and will be a leader in
fluorescent microscopy, image acquisition and image analysis of cell culture
and tissue models of disease.

Experience/Skills/Education required:

Expertise in confocal microscopy and fluorescent microscopy required. The
individual must have a good working knowledge of molecular biology, cell
biology and cell culture, and histology. Strong computer skills are a must,
with expertise in digital image acquisition and image analysis using
Metamorph, Image Pro Plus and Photoshop. Highly motivated self-starter,
with effective supervising skills. Excellent interpersonal skills, written
and oral presentation skills and team approach to research are a necessity,
as this individual will be interfacing with multiple teams throughout the
company. Ph.D. in cell and molecular biology, with 0-3 years post-doctoral
experience. Prior industrial experience a plus.

Please send resumes by US mail ONLY. Please do not reply to this message.
Resumes sent by email will NOT be considered.

______________________________
B. Thurberg, M.D., Ph.D.
Associate Director of Pathology
Preclinical Biology
Genzyme Corporation
One Mountain Road
P.O. Box 9322
Framingham, MA 01701-9322



From daemon Tue Feb 27 16:49:35 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 27 Feb 2001 16:49:28 -0600
Subject: SEM of Fungus

Contents Retrieved from Microscopy Listserver Archives
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I have looked extensively at a wild strain of Aspergillus many years ago. I
grew the fungus on sterilized glass slides with half-strength
saubourod-dextrose agar for at t least two days and up to 21 days. I cut
out pieces of the agar culture and fixed in 1% osmium fumes fix overnight
under cover, then a standard dehydration in ETOH, two changes of amyl
acetate and then critical point drying. These were sputter coated several
times as these were difficult to view without charging. I never published
this, but I dragged the paper out recently after 22 or so years to see if I
could do SEM on Candida. Funny you should also ask! Good luck and if you
have any other question, you can reach me at Bplowman-at-sfuop.edu.

Barbara L. Plowman
Univ. of the Pacific School of Dentistry
2155 Webster Rm 632
San Francisco, CA 94115
ph: 415-929-6692
FAX: 415-929-6654
email: Bplowman-at-sfuop.edu




From daemon Tue Feb 27 17:36:22 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 27 Feb 2001 18:31:47 -0500
Subject: Current level for LaB6 filaments

Contents Retrieved from Microscopy Listserver Archives
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Can anyone tell me if the required currents for the different brands of LaB6 filaments are significantly different?

The reason that I am asking this is that it appears that on my JEOL 2000FX that I can not fully saturate the LaB6 filament when it is set up correctly, i.e. tip to whenelt distance, centering, biasing. There appears to be a limit at which no more current is available as you turn up the filament current. If I lower the bias, I can saturate the filament, but the emission is too low. I seem to recall a discussion some time ago about whether the filament power on some of the older 2000FX had enough juice to do the job, but I may be mistaken.

I am using a new Denka filament. I have a new titanium wehnelt. It is doing the same as a used and a new Denka filament that I had in using the old SS wehnelt assembly. There are no problems when a tungsten filament is used. My thought is that if another brand of filament requires less current to heat (say about the same as the tungsten filament) that I might be able to use that.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Tue Feb 27 17:46:31 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Tue, 27 Feb 2001 18:28:21 -0600
Subject: Just For Fun Micrograph Contest

Contents Retrieved from Microscopy Listserver Archives
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Group,
At the upcoming M&M Conference in Long Beach (5/9 August), Microscopy Today
will again hold a "Just For Fun Micrograph Contest". Prizes will be $300,
$200 and $100 respectively for first second and third prizes. If interested
in participating, kindly contact me off line and I will provide complete
information.
See you in Long Beach,
Don Grimes, Microscopy Today



From daemon Tue Feb 27 19:15:47 2001



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au
Date: Wed, 28 Feb 2001 12:10:09 +1100
Subject: Clearing plant tissue

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I was wanting to know whether anyone has used methyl salicylate to clear
fixed slices of plant tissue, and if so, what in their experience is the
best method to use.

Thanks in advance,

Mark Talbot.



From daemon Tue Feb 27 21:03:55 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Tue, 27 Feb 2001 21:02:25 -0600
Subject: stainless steel crystallizing dishes

Contents Retrieved from Microscopy Listserver Archives
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Colleagues:

Does anyone know of a source of stainless steel crystallizing
dishes, 100-250 ml to use in electrolytic etching experiments in
metallography?

Thanks in advance!

Sam Purdy
Technical Center
National Steel Corp.
Trenton MI 48138
Voice 734-676-2682
FAX 734-676-2030
e-mail spurdy-at-nationalsteel.com




From daemon Tue Feb 27 21:46:06 2001



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Wed, 28 Feb 2001 14:43:26 +1100
Subject: JEOL 2000fxII aberration coefficients

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

a colleague would like to simulate HRES images to compare with those
recorded using our JEOL 2000fxII TEM. For this he needs to know the
objective lens spherical aberration coefficient (Cs). The polepiece
we have is the AHP20L. Does anyone know what the Cs value should be
for this instrument?

Another parameter he requires is the beam semi-convergence (in mrad).
How is this parameter defined and how can we measure it?

I would appreciate any advice you can offer. Cheers,

Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily
represent the official views of ANSTO from which this message was
conveyed.


From daemon Wed Feb 28 01:16:38 2001



From: Alexander Tikhonovski :      tikhonov-at-mpi-halle.mpg.de
Date: Wed, 28 Feb 2001 08:13:06 +0100
Subject: Re: Tripod Polisher

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopists,
}
} I am looking for a nice glue to fix the specimen to the tripod polisher for
} wedge polishing. The "super glue" stuff does not work well. The specimen falls
} off too easily.
}
} I would appreciate very much if anyone could send me some good idea.
}
} Best Regards
} Csaba Cserhati
}
Can you fix your specimen with epoxy resin?

I like to glue specimens with epoxy resin because the substance
does not harden too fast, and you always have time to find the best
location for your sample, so that the latter does not break.

Alex




From daemon Wed Feb 28 04:13:25 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 28 Feb 2001 10:01:28 +0000 (GMT Standard Time)
Subject: Re: RE: SEM - CPD

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Re what % ethanol to use for storage.

I once read a study which claimed 100% was better that the
usual 70%. I cannot remember the authors etc.

Dave


On Tue, 27 Feb 2001 21:39:29 +1000 Jim at ProSciTech
{jim-at-proscitech.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} If those cells were for TEM sections, I would be very concerned and re-hydrate
} them back to 70% alcohol immediately. For SEM lipid extractions matters less
} but you may well get some shrinkage. Its less urgent, but I would take those
} cells back to 70%. At that and refrigerated you SHOULD see no real change for
} some weeks.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw
} [SMTP:Bart.DePauw-at-rug.ac.be] wrote:
} }
} } Hello Everyone,
} }
} } This week, our CPD was broken. I was planning to CPD some samples that
} } day. The samples are now in acetone. My question : how long can these
} } samples stay in acetone ? The samples are epithelial cells from duodenum
} } grown on glass.
} }
} } greetings,
} }
} } De Pauw Bart
} } Belgium
} }
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Feb 28 04:47:26 2001



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Wed, 28 Feb 2001 12:43:34 +0200
Subject: Re: Clearing plant tissue

Contents Retrieved from Microscopy Listserver Archives
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Mark
A long time ago I needed to clear corn roots with attached parasitic
roots to make it possible to trace vascular connections between host
and parasite.
If I remember correctly the process I used employed xylol instead of
salicylate:

Decolorize and soften material in 1% hypochlorite bleach or in 0.5M
NaOH. Time (hours to days) and choice of bleach or alkali depends on
thickness and colour of the material.

After material is sufficiently translucent, dehydrate in ethanol to
100% and clear by immersing in xylol (take all precautions with this).
My recollection is that some materials became glassy clear, to such an
extent that it was necessary to stain the vascular bundles with a
trace of Safranin O during the 100% ethanol step.

Jan


MARK JEFFREY TALBOT wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} I was wanting to know whether anyone has used methyl salicylate to clear
} fixed slices of plant tissue, and if so, what in their experience is the
} best method to use.
}
} Thanks in advance,
}
} Mark Talbot.

--
Prof Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


From daemon Wed Feb 28 05:03:17 2001



From: Joakim.Fagerlund-at-skf.com
Date: Wed, 28 Feb 2001 11:52:45 +0100
Subject: Automatic SEM analysis of inclusions

Contents Retrieved from Microscopy Listserver Archives
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I am interested in a SEM that automatically can detect
and quantify the number and chemical composition of
inclusions present on a polished steel surface.

The SEM should manage to scan an area automatically
and detect all the inclusions, which fulfill some user defined
requirements e.g. a size over a certain limit or a special
chemical composition

Does anybody have experience of such system and
what manufactures are there ?

Best regards
Joakim Fagerlund






From daemon Wed Feb 28 05:04:00 2001



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Wed, 28 Feb 2001 10:48:51 -0000
Subject: Microscopy Meeting in London

Contents Retrieved from Microscopy Listserver Archives
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This is a multi-part message in MIME format.

------=_NextPart_000_01C0A174.09670A80
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 7bit

Dear Listers,

The Society of Electron Microscope Technology (SEMT), is holding a one day
meeting in London (England) on 28th March 2001 to include the following
topics:

Cryo TEM
Low Vacuum/Low Voltage Microscopy
GFP
Stereology
Confocal and 2-Photon Microscopy
Digital Imaging in Microscopy

All are welcome and further details are shown on the attachment. As a
committee member I can supply more information but it might be quicker to
go direct to the secretary David McCarthy, e-mail address also on the
attachment,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
------=_NextPart_000_01C0A174.09670A80
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------=_NextPart_000_01C0A174.09670A80--



From daemon Wed Feb 28 07:12:59 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 28 Feb 2001 07:03:17 -0600
Subject: RE: Automatic SEM analysis of inclusions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most EDS manufacturers offer this function as a part of their software.
Defining the inclusion positions is a key issue. Most often this is done
using BSE imaging by choosing a grayscale range (from histogram) of
inclusions while excluding the matrix. It may be an option for some. The
most simple method uses beam sweep control by the EDS/imaging system. Some
"integrated" EDS/SEM systems may also offer stage control. Haven't looked
in detail at the various manufacturer's specs for a while, so hesitate to be
more detailed. They, I am sure, would be glad to give you the details!

Woody

} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
}
} I am interested in a SEM that automatically can detect
} and quantify the number and chemical composition of
} inclusions present on a polished steel surface.
}
} The SEM should manage to scan an area automatically
} and detect all the inclusions, which fulfill some user defined
} requirements e.g. a size over a certain limit or a special
} chemical composition
}
} Does anybody have experience of such system and
} what manufactures are there ?
}
} Best regards
} Joakim Fagerlund
}
}
}
}
}


From daemon Wed Feb 28 07:38:10 2001



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 28 Feb 2001 08:35:14 -0800
Subject: Re: Tripod Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Csaba,

I agree with Scott (below). I've mangled a number of samples, in various ways, learning the tripod technique but have never had a complete (rare partial de-wets can be repaired) glue failure using the Loctite product. It's available in a "pen" type applicator which seems to have the best bench life of any of the applicators I've used. My tube usually is swiped off the bench long before it goes bad. One of the advantages to the cyanoacrylic cement is it
dissolves, in a reasonable time, in acetone. If you were to use a crosslinked epoxy, you'll need to devise a cleaning scheme that will exhaustively remove the epoxy without altering your sample. Good luck.

John


John W. Heckman
MSM Department
3505 Engineering Building
Michigan State University
East Lansing, MI 48824-1226
heckman-at-pilot.msu.edu

517-353-9719 (work)
517-353-9842 (dept. fax)



"Walck, Scott D." wrote:

}
} There has been a lot of discussion over the past on this subject. The boys from IBM are the experts. (There's your plug, Ron.) Since nobody has answered this yet, I'll give you my summary. Ron, If I say anything wrong, please correct me.
}
} Don't use any type of super glue gel! It doesn't work, you have to use the thin stuff.
}
} The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes difficult to locate in other parts of the country.
}
} I have had good success with the Alpha superglue from Electron Microscopy Sciences and with Loctite superglue available almost anywhere. Buy it from a place that has a high turnover rate of it so that it is fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator for any length of time. Once it has been opened, you can't use it for very long (a day or two). I have used the Loctite a little longer because it has a very good sealing top.
}
} I think that pressure on the sample while it cures is important, but the IBM guys just wick the extra stuff away from the sample and don't put a lot of pressure on the sample.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}



From daemon Wed Feb 28 08:06:26 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Wed, 28 Feb 2001 09:16:23 -0500
Subject: RE: Tripod Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Thanks for the plug, Scott.

I responded to the requester off line and said that most any super glue
works well. I should have added that "super glue" is defined as a
"cyanoacrylate," which should be printed on the bottle. We've never tried
a "gel' but if Scott doesn't like it that's good enough for us. The main
emphasis of my response was the absolute requirement for a clean surface
for the glue to bond to. Lack of cleanliness is the biggest cause of
specimens "falling off too easily." The bonding surfaces of the tool and
the specimen *must* be free of any contamination films. Inspect the
surfaces with reflected light. Water from the tap and impure solvents are
to blame.

With regard to the glue. We stopped using Ross awhile back. Currently we
have Loctite and ND Industries Super Glue in the lab.

Ron



Don't use any type of super glue gel! It doesn't work, you have to use the
thin stuff.

The IBM folks (Ron et al.) recommend Ross super glue, but that is sometimes
difficult to locate in other parts of the country.

I have had good success with the Alpha superglue from Electron Microscopy
Sciences and with Loctite superglue available almost anywhere. Buy it from
a place that has a high turnover rate of it so that it is fresh. The IBM
guys recommend keeping the stuff unopened in the refrigerator for any
length of time. Once it has been opened, you can't use it for very long (a
day or two). I have used the Loctite a little longer because it has a very
good sealing top.

I think that pressure on the sample while it cures is important, but the
IBM guys just wick the extra stuff away from the sample and don't put a lot
of pressure on the sample.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Csaba Cserhati [mailto:cserhati-at-delfin.klte.hu]
Sent: Tuesday, February 27, 2001 4:22 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Tripod Polisher


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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---------------------------------------------------------------
--------.


Dear Microscopists,

I am looking for a nice glue to fix the specimen to the tripod
polisher for
wedge polishing. The "super glue" stuff does not work well.
The specimen falls
off too easily.

I would appreciate very much if anyone could send me some good idea.

Best Regards
Csaba Cserhati







From daemon Wed Feb 28 08:56:27 2001



From: Kuusisto, Ari :      Ari.Kuusisto-at-perkinelmer.com
Date: Wed, 28 Feb 2001 08:55:44 -0600
Subject: Nikon DXM1200 and "mechanical" interpolation to increase the fina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I would like to ask some basic things of those cameras that scan the CCD to
get more resolution than the specific CCD really offers. I can understand
that this can work with color CCDs that have the color mask (array or mosaic
or whatever it is called) on the chip and cannot reach the full resolution
of the CCD. However, according to my knowledge the "scanning" cannot make
the resolution better than with the same chip used as black-and-white camera
(without the color mask). So the scanning is done just to compensate against
the loss of resolution caused by the color mask. Or has somebody really
invented some new rules. Could somebody please advise me, if I have missed
something here.

Ari Kuusisto, Research Physicist

PerkinElmer Life Sciences,
Wallac Oy

Phone: +358 2 267 8508 (direct)
+358 2 267 8111 (switchboard)
Fax: +358 2 267 8380
e-mail: Ari.Kuusisto-at-wallac.fi {mailto:Ari.Kuusisto-at-wallac.fi}
{mailto:Ari.Kuusisto-at-wallac.fi {mailto:Ari.Kuusisto-at-wallac.fi} }
Address: Wallac Oy
PO Box 10
FIN-20101 Turku, Finland
Homepage: www.wallac.fi {http://www.wallac.fi} {http://www.wallac.fi
{http://www.wallac.fi} }


-----Original Message-----
From: Hisashi Okugawa [SMTP:okugawa.h-at-nikon.co.jp]
{mailto:[SMTP:okugawa.h-at-nikon.co.jp]}
Sent: Tuesday, February 27, 2001 12:18 PM
To: Giles, Bill; Gary Gaugler
Cc: MSA listserver
Subject: Re: Metallograph and Digital Camera for Light
Microscopy


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
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{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}

-----------------------------------------------------------------------.


Nikon DXM1200 has 1.3M pixels CCD. With 3 x 3 times "Diractor",
it makes 12M pixels images. Because of the color array, 2 pixels
should
be moved totally. DXM1200 moves two times every two thirds pixels.
Pixera
moves 1 time every one pixel or three times every half pixels.
Please visit the following web to get more information

http://www.microscopyu.com/ {http://www.microscopyu.com/}

Hisashi Okugawa
1st design department
Instrument company in Nikon Corporation
Phone: 81-45-853-8568
Fax: 81-45-853-8475
e-mail: okugawa.h-at-nikon.co.jp {mailto:okugawa.h-at-nikon.co.jp}
----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com {mailto:gary-at-gaugler.com} }
To: "Giles, Bill" {William.Giles-at-TIMET.com
{mailto:William.Giles-at-TIMET.com} }
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com
{mailto:Microscopy-at-sparc5.microscopy.com} }
Sent: Tuesday, February 27, 2001 6:52 AM
Subject: RE: Metallograph and Digital Camera for Light Microscopy


}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
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{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
}
-----------------------------------------------------------------------.
}
}
} The Nikon MX1200 is native 1.2M pixels. It, like current
} generation digital microscope cameras, do a "mechanical"
} interpolation to increase final pixel count. One way is
} to physically move the CCD imager. Each movement
} results in new image data for that position. This is a bad
} way to gather more pixels, however. The other way is to
} keep the CCD fixed but scan the image across it multiple
} times at different angles.
}
} Zeiss has tried to do mechanical shifting and not done
} all that good of a job. Looks like Nikon is trying it too.
} They are up against a basic patent by Pixera. Pixera
} uses what they call a "Diractor" to scan the image
} across a fixed CCD.
}
} http://www.pixera.com/PixeraCatalog/Penguin/Penguin.htm
{http://www.pixera.com/PixeraCatalog/Penguin/Penguin.htm}
}
} The Pixera is available as a 1.5M pixel unit or as a 5.8M pixel
} Diractor unit. Each are also available with Peltier cooling.
} Like the Nikon, the Pixera uses a dedicated PCI card for
} PC or Mac. This card, camera and software will do
} live focus at 12-15 fps on a 733MHz system. It does
} manual and automatic exposure and also provides a focus
} indication bar. Also provided is automatic or manual
} white balance, selectable black balance and multi-size
} spot exposure metering.
}
} The best way to find out how well a camera performs is
} to take a high resolution shot at different lighting extremes
} and do an FFT on the images. Artifacts and garbage will
} show up if the system produces high pixel count at the
} expense of image quality.
}
} I use a Pixera Penguin 600CL and like it very much. It
} is easy to take single shots either as one exposure or
} averaged or integrated up to 256 exposures. Averaging
} and Peltier cooling greatly reduces ultimate noise in the
} image. For simple documentation purposes, this is
} not necessary. But for images which will undergo
} further processing, noise and artifacts must be reduced
} or eliminated. The Pixera has selectable ISO from 50 to 400.
} I run it at ISO 100. No info on what the Nikon does.
}
} The Pixera also supports Twain interfacing.
}
} gary g.
}
} At 08:57 AM 2/26/01, you wrote:
}
} } Lawrence,
} }
} } Thanks for your response.
} }
} } I read the literature available on the indicated web site and
have a
couple
} } of questions.
} }
} } There seems to be some conflicting information.
} } Under specifications:
} } Image size 3840 x 3072
} } CCD 1.34 M pixels
} } Document text: 12 million pixels (3046 x 3072) ???????
} }
} } Is my math wrong or am I missing some pertinent information?
} }
} } How do you obtain RGB? What does the color array on the chip look
like?
Or
} } does the camera use filters?
} }
} } What's the s/n ratio?
} }
} } Pixel size?
} }
} } Are there twain drivers available or a Photoshop plugin?
} }
} } Is any annotaion, image enhancement and/or image analysis
software
included?
} }
} } BTW, the Spot RT is $7k also, not twice that price.
} }
} } William T. Giles
} } Sr. Electron Microscopist
} } Met. Lab. Coordinator
} } Henderson Technical Laboratory
} } TIMET
} } PO Box 2128 Henderson NV 89009
} } Ph: (702)566-4436
} } Fax: (702)564-9038
} } E-mail: Bill.Giles-at-timet.com {mailto:Bill.Giles-at-timet.com}
} }
} } } -----Original Message-----
} } } From: Lawrence Kordon [SMTP:nikon-at-jagunet.com]
{mailto:[SMTP:nikon-at-jagunet.com]}
} } } Sent: Friday, February 23, 2001 1:38 PM
} } } To: Giles, Bill
} } } Cc: 'Microscopy-at-MSA.Microscopy.Com'
} } } Subject: Re: Metallograph and Digital Camera for Light
Microscopy
} } }
} } } William,
} } }
} } } Excellent choice on the Nikon Epiphot 200! However, why are
you not
} } } considering
} } } the Nikon DXM1200 Digital Camera? It is a whopping 12 Million
pixels
with
} } } a live
} } } Video Output to a PC and cost under $7000. That is about the
half the
} } } price of
} } } the Spot-RT. Also, why would you want to have a cooled CCD
anyway?
} } } Metallurgy;
} } } even with Fluorescence and high Mag DIC (Pol) does not require
it. Not
to
} } } mention this camera can do extremely low light anyway! This
Camera is
} } } literally
} } } the hottest product in Microscopy today! We have sold hundreds
already
} } } "sight/unseen" and are first shipping them. All the major
Image
Analysis
} } } companies have or are writing Drivers and it is so popular we
have put
it
} } } on
} } } other brands microscopes as well! By the way, the US MINT and
some
NIST
} } } Labs
} } } here is Washington, DC have it. Please check out the specs
at....
} } }

http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM
{http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=DXM}

} } } 1200
} } } and call you local Nikon Dealer for a Demonstration and
Quotation.
} } }
} } } Good Luck,
} } }
} } } Lawrence Kordon
} } } Nikon Instruments, Inc.
} } } Columbia, MD
} } } nikon-at-jagunet.com {mailto:nikon-at-jagunet.com}
} } }
} } }
} } }
} } } "Giles, Bill" wrote:
} } }
} } }
}
------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
of
America
} } } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}

} } } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
} } }
}
-----------------------------------------------------------------------.
} } } }
} } } } Good Day Listers,
} } } }
} } } } I am in the wonderful position of purchasing a new inverted
metallograph
} } } } with digital image acquisition for our laboratory.
} } } }
} } } } I have pretty much decided on the Nikon Epiphot 200
metallograph
with
} } } } brightfield and polarized light modules. Also chosen were
the Plan
} } } Achromat
} } } } objectives. Any comments on this choice?
} } } }
} } } } Now the real question. Which digital camera would you buy? I
know
these
} } } } things have been discussed greatly here in the past and I
have
followed
} } } them
} } } } with great interest.
} } } }
} } } } One of our primary wants is live image preview(} 15 fps).
} } } }
} } } } We are leaning towards the Spot RT, although the Insight
looks to be
} } } equally
} } } } promising.
} } } }
} } } } Any comments for or against either of these would be
appreciated.
Also,
} } } if
} } } } anyone has another preference I would appreciate hearing
your
comments.
} } } }
} } } } Thanks
} } } }
} } } } William T. Giles
} } } } Sr. Electron Microscopist
} } } } Met. Lab. Coordinator
} } } } Henderson Technical Laboratory
} } } } TIMET
} } } } PO Box 2128 Henderson NV 89009
} } } } Ph: (702)566-4436
} } } } Fax: (702)564-9038
} } } } E-mail: Bill.Giles-at-timet.com {mailto:Bill.Giles-at-timet.com}
}
}




From daemon Wed Feb 28 09:09:30 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 28 Feb 2001 09:09:29 -0600
Subject: Administrivia: Attachments and Apologies.....

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Sorry Folks...

An Email attachment got through the filter this AM.

Although it was a microscopy relevant posting it still
contained an attachment. This message about a meeting
in London managed to find it's way through a loop-hole
in the filter software. I believe that I have plugged that hole now.
(I guess I should thank you Terry, since your posting allowed
me to find the loop-hole).

As a safety issue let me remind you that you should scan
all Email attachments for viruses before you open them as it
is possible for viruses to be attached to messages even
if the author did not intend it.

As a safety issue, we "try" to reject all postings
having attachments and succeed most times. Please
remember to send all your Email messages
as simple ASCII text embedded in the body of your message.
Do not embedd HTML in your messages as some Email
programs (MS Outlook in particular) converts this
to an attachment in many situations, if this happens then
as an attachment the HTML embedded message will then get rejected.

In case your curious, of the 2, 234 messages have triggered
the Filter and been rejected in the last 2 years. The vast majority
of these were true junk mail.

Remember if you post a message and it gets caught and tagged by the filter
as suspect mail, READ THE REJECTION MESSAGE and then follow
the directions so that I can sort out the problem related to your
posting . Most of the rejections are true junk mail, but a few
of you will get caught by accident, this is the price we all have
to pay due to the junk mailers.


Cheers...
Nestor
Your Friendly Neighborhood SysOp





From daemon Wed Feb 28 10:11:51 2001



From: Massimo Sassaroli :      massimo-at-inka.mssm.edu
Date: Wed, 28 Feb 2001 11:06:07 -0500
Subject: Apologies -- Test message

Contents Retrieved from Microscopy Listserver Archives
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I apologize for this message. I have not been receiving messages from the
list since the second week in February and I am trying to establish why.
Again my apologies for cluttering your email boxes.

____________________________________________
Massimo Sassaroli
Dept. of Physiology and Biophysics, Box 1218
Mount Sinai School of Medicine
One Gustave L. Levy Place
New York, NY 10029
Tel:(212) 241-9512 FAX:(212) 860-3369
email: massimo-at-inka.mssm.edu



From daemon Wed Feb 28 10:25:59 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 28 Feb 2001 10:21:24 -0600
Subject: RE: JEOL 2000fxII aberration coefficients

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Mark,

You should be able to get the Cs value from JEOL.

On the convergence semi-angle, you can measure this in the diffraction
pattern IF you are working close to crossover (i.e. beam at crossover on the
specimen). Then, the diffraction pattern will consist of disks, and the
semi-angle for convergence is the radius of the disks, which you can convert
to angle in radians by scaling with respect to the other reflections in the
pattern (when you index, you get the Bragg angle if the lattice parameters
are known). This angle will scale with the condenser aperture radius. (For
more detail, see O'Keefe and Sanders, Acta Cryst A31 (1975) p. 307.)

It is likely that on a modern TEM you are not working very close to beam
crossover on the sample. You may safely assume that the convergence at
crossover is an upper limit. However, when you decondense the beam you are
producing a situation where incident angle varies as a function of position
on the sample, and locally the convergence may be much smaller than the
total convergence over the entire illuminated area. In the back focal
plane, each disk now contains an image of the sample, which goes together
with the previous statement.

If you refocus the diffraction pattern so that each spot is a sharp filament
image you will see that decondensing demagnfies the filament, so it is
(locally) decreasing the convergence angle significantly. The convergence
angle now scales not with the condenser aperture but with the spot size. To
measure it you may refocus the diffraction pattern to make each spot an
in-focus filament image, then measure the semi-angle of the filament images.


I hope this is not more confusing here than helpful!

Regards,

Wharton Sinkler
UOP LLC
Des Plaines, IL


} -----Original Message-----
} From: Mark Blackford [SMTP:mgb-at-ansto.gov.au]
} Sent: Tuesday, February 27, 2001 9:43 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: JEOL 2000fxII aberration coefficients
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
}
} a colleague would like to simulate HRES images to compare with those
} recorded using our JEOL 2000fxII TEM. For this he needs to know the
} objective lens spherical aberration coefficient (Cs). The polepiece
} we have is the AHP20L. Does anyone know what the Cs value should be
} for this instrument?
}
} Another parameter he requires is the beam semi-convergence (in mrad).
} How is this parameter defined and how can we measure it?
}
} I would appreciate any advice you can offer. Cheers,
}
} Mark Blackford
} TEM Group
} Materials Division, ANSTO
} PMB 1,
} Menai, N.S.W.
} Australia
} 2234
}
} Phone 61 2 9717 3027
} Fax 61 2 9543 7179
}
} Disclaimer:
} The views expressed in this E-mail message do not necessarily
} represent the official views of ANSTO from which this message was
} conveyed.


From daemon Wed Feb 28 10:50:55 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Wed, 28 Feb 2001 17:45:42 +0100
Subject: Jeol 2000fxII aberration coefficients

Contents Retrieved from Microscopy Listserver Archives
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Dear Mark,

the sperical aberration constans for JEOL 2000FXII AHP20L is:

Cs=3.4mm

Cheers,
Csaba

--
____________________________________________
Csaba Cserhati
Univ.of Debrecen / Dept. of Solid State Phys.
Hungary
tel/fax: 36 52 316073
e-mail: cserhati-at-delfin.klte.hu
____________________________________________


From daemon Wed Feb 28 10:55:59 2001



From: Seijo, Edward R. :      SeijoER-at-moffitt.usf.edu
Date: Wed, 28 Feb 2001 11:53:01 -0500
Subject: Looking for a Stage Micrometer

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Looking for a stage micrometer that will allow me to calibrate down to the
micrometer level. Everything I have found thus far is 1mm.

Thanks


From daemon Wed Feb 28 11:07:43 2001



From: Rodney McCabe :      rmccabe-at-lanl.gov
Date: Wed, 28 Feb 2001 10:03:56 -0700
Subject: Quantitative stereo TEM

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Dear Colleagues:

I am interested in doing quantitative stereo microscopy using a TEM and am
looking for software that may be helpful in such an endeavor. I anticipate
the primary application will be examining the spatial arrangement of linear
defects. I have not been able to find a software designed directly for
quantitative stereo TEM. I have come across one commercial software that
can be used for SEM surface topography that may be applicable. It seems
like there must be other software packages out there used for applications
such as surface mapping, electron tomography, or even astronomy that could
be useful. Also, as I am new to stereo microscopy, any words of wisdom
would be appreciated.

Thanks,

Rod




From daemon Wed Feb 28 11:14:03 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 28 Feb 2001 12:09:46 -0500
Subject: Re: Nikon DXM1200 and "mechanical" interpolation to increase the

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I would like to ask some basic things of those cameras that scan the CCD to
get more resolution than the specific CCD really offers. I can understand
that this can work with color CCDs that have the color mask (array or mosaic
or whatever it is called) on the chip and cannot reach the full resolution
of the CCD. However, according to my knowledge the "scanning" cannot make
the resolution better than with the same chip used as black-and-white camera
(without the color mask). So the scanning is done just to compensate against
the loss of resolution caused by the color mask. Or has somebody really
invented some new rules. Could somebody please advise me, if I have missed
something here.

Dear Ari,
You are, indeed, missing something. I expect that there is a less obscure
referrence, but the one that I know of is a paper by Atsushi Imiya in Vol. 101
(1997) of Advances in Imaging and Electron Physics. The paper, Formal
Polynomials for Image Processing, goes from p. 99-142, and includes a section
from p. 125-135 on subpixel resolution and pyramid transforms. The author's
address is Department of Information and Computer Sciences, Faculty of
Engineering, Chiba University, Yayoi-cho, Chiba 260, Japan. If you can't find
the volume, you can write for a reprint.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Wed Feb 28 11:15:03 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 28 Feb 2001 17:11:58 +0000 (GMT Standard Time)
Subject: Database

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Could anyone please recommend me a database with manufacturers / suppliers
for a polarizing optical microscope with reflectance mode and Nomarski
attachment? Any experience of a recent purchase would also be welcome.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+



From daemon Wed Feb 28 11:24:24 2001



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Wed, 28 Feb 2001 09:23:02 -0800
Subject: Re: JEOL 2000fxII aberration coefficients

Contents Retrieved from Microscopy Listserver Archives
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Hi Mark,

I can't help you with your Cs value, but the incident-beam convergence
semi-angle can be measured from a diffraction pattern taken with the beam
adjusted as for imaging. With luck you should see a disk at each
diffraction spot [1,2], and the radius of the disk is the semiangle. If you
use a specimen with a known d-spacing in a known orientation, you will be
able to scale the diffraction pattern in angle -- remember that 2.theta =
n.lambda/d and get the semiangle in milliradian. Then you need to decide if
the disks have uniforn illumination across them, or if the intensity is
greater in the center. If you think that the intensity distribution is more
like a 2-D Gaussian, use a HREM-simulation program that uses a Gaussian
model for the convergence. If you think the disks are uniform, then use a
HREM-simulation program that uses a "top-hat" model for the convergence. If
the disks are uniform, but you are stuck with a HREM-simulation program that
uses a Gaussian model for the convergence, make sure that the std.dev. of
the Gaussian (its sigma) is set to 0.77 times the disk radius [2].
[1] "n-beam lattice images, VI. Degradation of image resolution by a
combination of incident-beam divergence and spherical aberration", M.A.
O'Keefe and J.V. Sanders, Acta Cryst. A31 (1975) 307-310.
[2] "Using convergence and spread-of-focus parameters to model spatial and
temporal coherence in HRTEM image simulations", Jan-Olle Malm and Michael A.
O'Keefe, in 51st Ann Proc. MSA, Cincinnatti, Ohio (1993) 974-975.
Cheers,
Mike O'Keefe

Mark Blackford wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All,
}
} a colleague would like to simulate HRES images to compare with those
} recorded using our JEOL 2000fxII TEM. For this he needs to know the
} objective lens spherical aberration coefficient (Cs). The polepiece
} we have is the AHP20L. Does anyone know what the Cs value should be
} for this instrument?
}
} Another parameter he requires is the beam semi-convergence (in mrad).
} How is this parameter defined and how can we measure it?
}
} I would appreciate any advice you can offer. Cheers,
}
} Mark Blackford
} TEM Group
} Materials Division, ANSTO
} PMB 1,
} Menai, N.S.W.
} Australia
} 2234
}
} Phone 61 2 9717 3027
} Fax 61 2 9543 7179
}
} Disclaimer:
} The views expressed in this E-mail message do not necessarily
} represent the official views of ANSTO from which this message was
} conveyed.



From daemon Wed Feb 28 12:28:52 2001



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Wed, 28 Feb 2001 12:25:24 -0600
Subject: DataBase/Vendor Listings on the WWW

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You can try the following

http://www.amc.anl.gov/Docs/NonANL/ComSites.html#Catag
}
scan down the list to the light/optical/confocal listings.

howwever, this listing is NOT differentiated by the specific
criteria you asked. It only represents vendors that have
submitted information and is therefore not necessairly
comprehensive.


Nestor

} }
} }
} }
} } Could anyone please recommend me a database with manufacturers / suppliers
} } for a polarizing optical microscope with reflectance mode and Nomarski
} } attachment? Any experience of a recent purchase would also be welcome.
} }
} } +------------------------------------------------------------------------+
} } | Robert H.Olley Phone: |
} } | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
} } | University of Reading {University internal extension 7867 |
} } | Whiteknights Fax +44 (0) 118 9750203 |
} } | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
} } | England URL: http://www.reading.ac.uk/~spsolley |
} } +------------------------------------------------------------------------+

===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================




From daemon Wed Feb 28 14:45:04 2001



From: Werner Anetseder :      anetseder-at-web.de
Date: Wed, 28 Feb 2001 21:39:51 +0100
Subject: preparation of fibers for SEM

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Dear Microscopists!

I'd like to get a closer look on coatings of silica-fibers using SEM. Has anybody ever tried to polish cross- and/or longitudinal sections?

Thanks for any information!
Werner
_______________________________________________________________________________
Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de
Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de



From daemon Wed Feb 28 14:46:41 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Wed, 28 Feb 2001 14:52:51 -0600
Subject: SEM of fungus

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Microlisters,

While the simpler and less time consuming suggestions already mentioned in
this thread may work in some cases, I found out a long time ago that the
only way to deal with the "fungal jungle", eg. fungi cultured on agar
plates, is to hit 'em with the ol' OTO method, preceded by glutaraldehye
fixation. Quick outline is as follows:

Glut. fixation, your favorite buffer
Buffer rinses
Osmium tetroxide, 2%
Water rinses
Thiocarbohydrazide, sat. soln.,filtered
water rinses
Osmium tetroxide, 2%
Water rinses
dehydration series, EtOH, or acetone
CPD
metal coating? Maybe, maybe not. Try without first.

This eliminates the charging, and fixation is quite good.

Classic reference for this method:

"Ligand-mediated osmium binding:Its application in coating biological
specimens for scanning electron microscopy". Kelley, Robert O., A.F. Dekker
and John G. Bluemink. J. Ultrastrucute Research 45:254-258 (1973).

See also:

"Non-coating techniques to render biological specimens conductive/1980
update". Judith A. Murphy. Scanning Electron Microscopy/1980 Vol.I, p.
209-220.


Good luck!

Gib Ahlstrand

-----------------------------------------------------------------
Rad0

You might try exposing your non-aquatic sample (bread molds, mushrooms,
etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4%
OsO4 on the lid of a very small container with your sample inside or the
lid over the sample directly), then air dry, mount, coat and view. As with
any sample, the drying artifacts vary with the fungus. Some things look
great.

good luck

Steve
} -----------------------------------------------------------------
} Hello,
}
} Forgive me, I haven't tried to find this on my own yet...
}
} But have any of you used an electron microscope to get a
} close-up of a fungus? Can this be done?
}
} Thanks...



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Wed Feb 28 15:19:41 2001



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Wed, 28 Feb 2001 14:14:00 -0700
Subject: RE: Nikon DXM1200 and "mechanical" interpolation to increase the

Contents Retrieved from Microscopy Listserver Archives
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Ari,

You've addressed my concerns exactly. Then again, you're not trying to sell
me a camera.

I tend to be wary of 12 megapixel output from a 1.34 megapixel chip, even if
"my" math is wrong.

I too am interested in how the interpolation is done in these cameras.

It appears that some manufactures are using controlled optics to shift the
image on the CCD, whilst others are shifting the CCD.
How that interpolation then happens would be most informative.
Here again, real info is difficult to come by on the "sales" oriented
sites.Pixera seems to offer more info than others.

It would seem that by using the Bayer mask, which uses 2 green tinted pixels
for 1 each of blue and red, that the resolution of the chip would decrease
fourfold.

Here again, i'm just an inquiring mind that would like to know how the
technology that we, the buying public, are being asked to believe.

William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com

} -----Original Message-----
} From: Kuusisto, Ari [SMTP:Ari.Kuusisto-at-perkinelmer.com]
} Sent: Wednesday, February 28, 2001 6:56 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Nikon DXM1200 and "mechanical" interpolation to increase the
} fina l pixel count
} Importance: High
}
} Hi all,
}
} I would like to ask some basic things of those cameras that scan the CCD
} to
} get more resolution than the specific CCD really offers. I can understand
} that this can work with color CCDs that have the color mask (array or
} mosaic
} or whatever it is called) on the chip and cannot reach the full resolution
} of the CCD. However, according to my knowledge the "scanning" cannot make
} the resolution better than with the same chip used as black-and-white
} camera
} (without the color mask). So the scanning is done just to compensate
} against
} the loss of resolution caused by the color mask. Or has somebody really
} invented some new rules. Could somebody please advise me, if I have missed
} something here.
}


From daemon Wed Feb 28 16:37:46 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 28 Feb 2001 10:45:10 -0600
Subject: History of TEM trivia questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listers,

I'm preparing a talk which is to include a timeline giving dates and names
for some relevant TEM developments. I'm trying to find answers to two
trivia questions with great importance to microscopy:

1) Who was the first to knowingly image a dislocation in a TEM, when was it
done and where was it published? On this, I have found references as far
back as an article by Heidenreich in J. Appl. Phys, 1949, but don't have
this journal going back that far.

2) Who developed the solid state detector for energy analysis of x-rays
(EDS), when and where published?

I have found some references to books on TEM history by Marton (1968) and
Hawkes (1985) but don't have these on hand.

Thanks,

Wharton

***********************
Wharton Sinkler
UOP LLC
Des Plaines, IL



From daemon Wed Feb 28 18:21:36 2001



From: HAMMOND,LOMA (HP-Corvallis,ex1) :      loma_hammond-at-hp.com
Date: Wed, 28 Feb 2001 16:07:22 -0800
Subject: LM: staining particles forensic type application

Contents Retrieved from Microscopy Listserver Archives
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Wharton,

Check out our web site we have the info.

http://srv.emunit.unsw.edu.au/


Barry
EMU
UNSW


----- Original Message -----
} From: Sinkler, Wharton {WSinkler-at-uop.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 01, 2001 3:45 AM



This is a forensic microscopy question. I would like a stain(s) that
differentiates between skin/protein particles and cellulose/starch
particles. I would like the stain to be evident in the compound microscope
under normal incident lighting. The particles are filtered from a solution
of IPA and captured on a .2um polycarbonate filter. The information I have
come across would suggest Ninhydrin for the staining of peptides in skin,
but I have not found any info on preferentially staining cellulose/paper
products.

Any suggestions,

Loma Hammond
IR/ Raman Engineer
Inkjet printing - Hewlett-Packard
loma_hammond-at-hp.com .


From daemon Wed Feb 28 18:42:20 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 1 Mar 2001 10:40:37 +1000
Subject: RE: Looking for a Stage Micrometer

Contents Retrieved from Microscopy Listserver Archives
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1mm? For that I'll use a ruler. One of our stage micrometers is 0.1mm long and
has 0.002mm divisions.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, March 01, 2001 2:53 AM, Seijo, Edward R.
[SMTP:SeijoER-at-moffitt.usf.edu] wrote:
}
}
} Looking for a stage micrometer that will allow me to calibrate down to the
} micrometer level. Everything I have found thus far is 1mm.
}
} Thanks



From daemon Wed Feb 28 19:27:23 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 1 Mar 2001 11:24:16 +1000
Subject: RE: RE: SEM - CPD

Contents Retrieved from Microscopy Listserver Archives
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That would be new to me. I would like to see that study.
It also would be counter-intuitive since conc alcohol and acetone are known to
extract lipids, whereas 50 to 70% hardly does. And since membranes are mostly
lipids . . .
Also during dehydration the greatest specimen shrinkage occurs above 80%
alcohol. So it makes sense to have more and smaller steps at the upper end, but
that is mostly due to physical effects.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, February 28, 2001 8:01 PM, Patton, David
[SMTP:David.Patton-at-uwe.ac.uk] wrote:
}
} Re what % ethanol to use for storage.
}
} I once read a study which claimed 100% was better that the
} usual 70%. I cannot remember the authors etc.
}
} Dave
}
}
} On Tue, 27 Feb 2001 21:39:29 +1000 Jim at ProSciTech
} {jim-at-proscitech.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } If those cells were for TEM sections, I would be very concerned and re-
} } hydrate
} } them back to 70% alcohol immediately. For SEM lipid extractions matters
less
} }
} } but you may well get some shrinkage. Its less urgent, but I would take
those
} }
} } cells back to 70%. At that and refrigerated you SHOULD see no real change
} } for
} } some weeks.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Tuesday, February 27, 2001 7:56 PM, Bart De Pauw
} } [SMTP:Bart.DePauw-at-rug.ac.be] wrote:
} } }
} } } Hello Everyone,
} } }
} } } This week, our CPD was broken. I was planning to CPD some samples that
} } } day. The samples are now in acetone. My question : how long can these
} } } samples stay in acetone ? The samples are epithelial cells from duodenum
} } } grown on glass.
} } }
} } } greetings,
} } }
} } } De Pauw Bart
} } } Belgium
} } }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}



From daemon Wed Feb 28 19:56:33 2001



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Wed, 28 Feb 2001 19:46:48 -0600
Subject: THIRD ANNOUNCEMENT: 6th Live-cell Course at UBC: June 18-28

Contents Retrieved from Microscopy Listserver Archives
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THIRD ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March 1!!

(Note: I know that today is the deadline but there were still some
vacancies last night and we will accept applications as long as this
remains true. In addition, there is a Waiting List to replace those
who find they cannot come for medical or financial reasons. JP)

Hello all,

We have two additions to the faculty for the 2000 UBC 3D Live-cell
Microscopy Course:

* Alan Hibbs from BioCon in Melbourne (who was really on the list to
start with but I somehow missed on the previous announcements. Sorry
Alan!)

* Mark Cannell from U. Auckland NZ. Well known for developments in
multiphoton microscopy but also for deconvolving 3D data from
confocal and multiphoton microcopes. Mark will replace Jason Swedlow
from U. Dundee who had schedule conflicts (Thanks for your help the
last 3 years Jason!)

Other faculty include:

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted Inoué Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Michael Weis Agriculture Canada


Basic info is below but you can get the entire brochure, including
links to faculty home pages, at

http://www.cs.ubc.ca/spider/ladic/course/brochure.htm

Cheers,

Jim P.

Sixth Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells
June 18 - 28, 2001


Fifth, Post-course Workshop on

3D Image Processing,
June 30 - July 3, 2001


Organized by Prof. James Pawley,
(University of Wisconsin-Madison)

in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada



DATES

Applications must be received by March 1, 2001
Deposit due April 15, 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001
3D Image Processing Wksp Sat. June 30 - Monday, July 2


APPLICATIONS DUE BY MARCH 1, 2001


More information at :
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or

REGARDING COURSE ORGANIZATION:

Contact:
Prof. James B. Pawley,
JBPAWLEY-at-FACSTAFF.WISC.EDU

REGARDING APPLICATIONS

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4


THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary improvement
in our ability to view living cells. To help convert this promise to
reality for a wider selection of biological scientists, an intensive
eleven-day residential course concentrating on all aspects of 3D Microscopy
of Living Cells will be held at the University of British Columbia, in June
of 2001. The course includes 4 days on 2D techniques, 6 days of 3D
techniques and a summary presentation. It covers basic microscopy to the
highest level confocal microscopy. (A half-day Pre-course is offered for
any who may need to brush up on basic optics!).

Topics include:
o Quantitative 2D light microscopy
o How to keep your cells alive
o 3D imaging in confocal microscopy
o Widefield/deconvolution techniques
o Two-photon excitation microscopy
o Fluorescent and backscattered light signals
o Dye design, characteristics and use
o Pixelation: The Nyquist Criterion
o Lasers and laser tweezers
o Objectives and aberrations
o Scanning-systems: AODs and mirrors
o Optimal pinhole size/photon efficiency
o Detectors: operation and performance
o Video-rate confocal imaging
o Measuring ion concentrations
o Display and measurement of 3D data

Morning lecture/demonstrations lead to hands-on laboratory exercises each
afternoon that will utilize most of the commercial instruments currently
available for 3D microscopic imaging. Students will work in groups of 3 or
4 throughout the discussion and laboratory sessions, and may complete a
live-cell 3D study on their own specimens. In the first five years, over
130 students from 23 countries have attended. Last year, 11 separate, 3D
microscopical workstations were available for student use under the
supervision of a 17-member international faculty. We expect to have even
more workstations in 2001. Including manufacturers representatives, the
teacher/ student ratio will be almost 2:1.

INTERNATIONAL FACULTY

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o mark Cannell Univ. of Auckland
o Elaine Humphrey Univ. of British Columbia
o Alan Hibbs BioCon, Melbourne
o Stephan Hell Max Planck, Goettingen
o Elaine Humphrey University of British Columbia
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Glen MacDonald University of Washington
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Michael Weis Agriculture Canada

TUITION

Course tuition is $2,150 US and includes lunches. On receipt of 50%
deposit, students will receive preliminary group assignments and the
textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The
tuition fee includes the textbook, a binder of handouts, and tickets for
the Opening Reception, the Manufacturer's Reception and the Beach Party.
Accommodations and meals other than lunch are not included in the tuition
fee. The Pre-course is $100 US.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrollment is limited to about 24 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts on
request to read before the course begins and are encouraged to take the
Pre-course on the afternoon of June 18.


Application forms, and other course information from this and past years,
can be downloaded from the WWW site at:

http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4
Phone: 1-604.822-3354
FAX: 1-604.265-5315
Email: ech-at-unixg.ubc.ca.


Application deadlines:

Application forms are due by March 1, 2000!
Deadlines are early to facilitate setting up groups. Successful applicants
will be notified by April 1, and a deposit of 50% must be received by April
15, 2000 to reserve your position. In general, deposit refunds are only be
possible if your position can be filled from the waiting list. The
remainder of the fees is due at registration.

DATES

Applications must be received by March 1, 2001
Deposit due April 15 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001


TEACHING PHILOSOPHY

As befits teaching in an area at the boundary of "what is now known,"
lecturers have been chosen based on their expertise as scientists working
in the field rather than because they all agree. They are encouraged more
to be provocative than to be prosaic. Students should expect discussion in
areas where differences of opinion exist.

Prior to the course, students will be organized into groups and encouraged
to communicate by email/phone, about the "Living-cell" group projects that
they will pursue during the course and that will be presented to the class
on the last day of the course. It has been found that group interactions
make best use of students' prior experience and can be very effective in
teaching the practical skills covered in a hands-on course of this type.

ARRANGEMENTS FOR LIVE SAMPLES

Students must contact Dr. Elaine Humphrey to make necessary arrangements
for the transport and maintenance of cell lines etc. needed for their
projects. Organisms linked in any way with human disease are not permitted
because of safety considerations. Transport and customs arrangements for
living specimens are entirely the responsibility of the student.
Students also attending the 3D Image Processing Course, may be able to
analyze, process and display some of the 3D data collected from their
specimens


********************************************************************************

Fourth Annual

3D MAGE PROCESSING WORKSHOP

The workshop will cover 3D image processing for measurement and display.
Enrollment is limited to those attending the 3D Microscopy course.
Tuition : $850 US (lunches and snacks incl.)

WHO SHOULD ATTEND?

The course is designed for biologists working with multidimensional and
possibly multicolor microscopical data sets. Getting the data is only half
of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to
store let alone analyze or display. This course is to help students
understand the hardware and software aspects of this problem and give them
the techniques they need to make the best use of their data.

The course is designed for biologists who need to make measurements on 3D
microscopical data sets and then display the results in an effective
manner. The course will be taught on SGI, Macintosh and IBM-compatible
computers. A wide variety of software designed for the 3D microscopy market
will be described, demonstrated and available for use.

Workshop Organizers

o Andreas Kriete Giessen University, DE
o Felix Margadant University of Sydney, Au
o Ping Chin Cheng State U. of New York, Buffalo
o Elaine Humphrey University of British Columbia
o Glen MacDonald University of Washington

PLAN OF INSTRUCTION
Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations
followed immediately by hands-on laboratory sessions using a variety of
workstations. Students will "learn-by-doing" with two to a machine. Lab
handouts will describe some specific exercises to be performed on "canned"
data sets. Facilities and supervision will be available until 11:00 PM, for
students to work on their own data.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Wed Feb 28 21:34:15 2001



From: erqla-at-163.net
Date: Thu, 01 Mar 2001 11:20:44 -0800
Subject: Judicial Judgements-Child Support {

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{HTML}
{BODY bgColor=3D#C0C0C0}

{FONT face=3D"Arial"}
{FONT size=3D4}
{FONT color=3D"#800040"} Thank you for your interest! {/FONT}
{FONT size=3D3}
{FONT color=3D"#800040"} {/FONT}
{FONT color=3D"#000000"} PI1 {BR}
{BR}
Judgment Courses offers an extensive Audio training {BR}
course in {/FONT}
{FONT color=3D"#0000A0"} "How to Collect Money Judgments" {/FONT}
{FONT color=3D"#000000"} . {BR}
{BR}
If you are like many people, you are not even sure what a {BR}
Money Judgment is and {/FONT}
{FONT color=3D"#0000A0"} why processing Money Judgments {BR}
can earn you very substantial income {/FONT}
{FONT color=3D"#000000"} . {BR}
{BR}
If you ever sue a company or a person and you win then you {BR}
will have a Money Judgment against them. {BR}
{BR}
You are happy you won but you will soon find out the {BR}
shocking fact: "Its now up to you to collect on the {BR}
Judgment". The court does not require the loser to pay you. {BR}
The court will not even help you. You must trace the loser {BR}
down, find their assets, their employment, bank accounts, {BR}
real estate, stocks and bonds, etc. {BR}
{BR}
Very few people know how to find these assets or what to do {BR}
when they are found. The result is that millions of {BR}
Judgments are just sitting in files and being forgotten. {BR}
{BR}
"In 79% of the cases the winner of a Judgment never sees a {BR}
dime." {BR}
{BR}
The non-payment of judicial debt has grown to epidemic {BR}
proportions. Right now in the United States there is {BR}
between {/FONT}
{FONT color=3D"#0000A0"} 200 and 300 billion dollars of uncollected Money {=
BR}
Judgment debt {/FONT}
{FONT color=3D"#000000"} . For every Judgment that is paid, 5 more {BR}
Judgments take its place. {BR}
{BR}
We identified this massive market 8 years ago and have {BR}
actively pursued Judicial Judgments since. We invented this {BR}
business. We have perfected it into a well proven and solid {BR}
profession in which only a select few will be trained in the {BR}
techniques necessary to succeed. {BR}
{BR}
With our first hand experience we have built a course which {BR}
teaches you how to start your business in this new unknown {BR}
and exciting field of processing Money Judgments. {BR}
{BR}
By following the steps laid out in our course and with {BR}
reasonable effort you can become very successful in the {BR}
processing of Money Judgments. {BR}
{BR}
The income potential is substantial in this profession. {/FONT}
{FONT color=3D"#0000A0"} We {BR}
have associates who have taken our course and are now {BR}
working full time making $96,000.00 to over $200,000.00 per {BR}
year. Part time associates are earning between $24,000.00 {BR}
and $100,000.00 per year {/FONT}
{FONT color=3D"#000000"} . Some choose to operate out of {BR}
their home and work by themselves. Others build a sizable {BR}
organization of 15 to 25 people in attractive business {BR}
offices. {BR}
{BR}
Today our company and our associates have over 126 {BR}
million dollars in Money Judgments that we are currently {BR}
processing. Of this 126 million, 25 million is in the form {BR}
of joint ventures between our firm and our associates. {BR}
Joint ventures are where we make our money. We only break {BR}
even when our course is purchased. We make a 12% margin on {BR}
the reports we supply to our associates. Our reporting {BR}
capability is so extensive that government agencies, police {BR}
officers, attorneys, credit agencies etc., all come to us {BR}
for reports. {BR}
{BR}
{BR}
Many of our associates already have real estate liens in {BR}
force of between 5 million to over 15 million dollars. {BR}
Legally this means that when the properties are sold or {BR}
refinanced our associate must be paid off. The norm is 10% {BR}
interest compounded annually on unpaid Money Judgments. {BR}
Annual interest on 5 million at 10% translates to {BR}
$500,000.00 annually in interest income, not counting the {BR}
payment of the principal. {BR}
{BR}
Our associates earn half of this amount or $250,000.00 per {BR}
year. This is just for interest, not counting principle {BR}
and not counting the compounding of the interest which can {BR}
add substantial additional income. Typically companies are {BR}
sold for 10 times earnings. Just based on simple interest {BR}
an associate with 5 million in real estate liens could sell {BR}
their business for approximately 2.5 million dollars. {BR}
{BR}
{/FONT}
{FONT color=3D"#0000A0"} 92% of all of our associates work out of their ho=
me; 43% {BR}
are women and 36% are part time {/FONT}
{FONT color=3D"#000000"} . {BR}
{BR}
One of the benefits of working in this field is that you are {BR}
not under any kind of time frame. If you decide to take off {BR}
for a month on vacation then go. The Judgments you are {BR}
working on will be there when you return. The Judgments {BR}
are still in force, they do not disappear. {BR}
{BR}
The way we train you is non-confrontational. You use your {BR}
computer and telephone to do most of the processing. You {BR}
never confront the debtor. The debtor doesn't know who you {BR}
are. You are not a collection agency. {BR}
{BR}
Simply stated the steps to successful Money Processing {BR}
are as follows: {BR}
{BR}
Mail our recommended letter to companies and individuals {BR}
with Money Judgments. (We train you how to find out who {BR}
to write to) {BR}
{BR}
8% to 11% of the firms and people you write will call you {BR}
and ask for your help. They call you, you don't call them {BR}
unless you want to. {BR}
{BR}
You send them an agreement (supplied in the course) to {BR}
sign which splits every dollar you collect 50% to you and {BR}
50% to them. This applies no matter if the judgment is for {BR}
$2,000.00 or $2,000,000.00. {BR}
{BR}
You then go on-line to our computers to find the debtor {BR}
and their assets. We offer over 120 powerful reports to {BR}
assist you. They range from credit reports from all three {BR}
credit bureaus, to bank account locates, employment {BR}
locates, skip traces and locating stocks and bonds, etc. {BR}
The prices of our reports are very low. Typically 1/2 to {BR}
1/3 of what other firms charge. For example we charge {BR}
$6.00 for an individuals credit report when some other {BR}
companies charge $25.00. {BR}
{BR}
Once you find the debtor and their assets you file {BR}
garnishments and liens on the assets you have located. {BR}
(Standard fill in the blanks forms are included in the {BR}
course) {BR}
{BR}
When you receive the assets you keep 50% and send 50% to {BR}
the original Judgment holder. {BR}
{BR}
Once the Judgment is fully paid you mail a Satisfaction of {BR}
Judgment to the court. (Included in the course) {BR}
{BR}
Quote's from several of our students: {BR}
{BR}
Thomas in area code 516 writes us: "I just wanted to drop {BR}
you a short note thanking you for your excellent course. {/FONT}
{FONT color=3D"#0000A0"} My {BR}
first week, part time, will net me 3,700.00 dollars {/FONT}
{FONT color=3D"#000000"} . Your {BR}
professionalism in both the manual and the video opened {BR}
doors for me in the future. There's no stopping me now. {BR}
Recently Thomas states he has over $8,500,000 worth of {BR}
judgments he is working on. {BR}
{BR}
After only having this course for four months, Larry S. in {BR}
area code 314 stated to us: " {/FONT}
{FONT color=3D"#0000A0"} I am now making $2,000.00 per {BR}
week {/FONT}
{FONT color=3D"#000000"} and expect this to grow to twice this amount with=
in the {BR}
next year. I am having a ball. I have over $250,000 in {BR}
judgments I am collecting on now." {BR}
{BR}
After having our course for 7 months Larry S. in 314 stated {BR}
" {/FONT}
{FONT color=3D"#0000A0"} I am now making $12,000.00 {/FONT}
{FONT color=3D"#000000"} per month and have approximately {BR}
$500,000.00 in judgments I am collecting on. Looks like I {BR}
will have to hire someone to help out" {BR}
{BR}
Marshal in area code 407 states to us "I feel bad, you only {BR}
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From daemon Thu Mar 1 00:19:58 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Thu, 1 Mar 2001 08:14:49 +0200
Subject: Re: History of TEM trivia questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Wharton

} I have found some references to books on TEM history by Marton (1968)
} and Hawkes (1985) but don't have these on hand.

I have a book that was published at the time of the International
Congress on EM in Kyoto, Japan, in 1986, entitled "History of
Electron Microscopes 1986" edited by Hiroshi Fujita. This gives a
lot of information on the history of EM, mainly from a Japanese
perspective. I haven't seen whether or not this has the answers to
your specific questions but if there is someone near you who has a
copy of this book you will find much interesting information in it.
Please let me know if you cannot get hold of a copy.


Robin H Cross (Mr)
Director : Electron Microscopy Unit
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
email: r.cross-at-ru.ac.za
http://www.ru.ac.za/emu/index.htm

** remember ICEM-15 in Durban in September 2002 **


From daemon Thu Mar 1 01:09:44 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 01 Mar 2001 08:17:33 +0100
Subject: Re: LM: staining particles forensic type application

Contents Retrieved from Microscopy Listserver Archives
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Hi

I am not a forensic scientist so my suggestions are general - therefore I
am uncertain how the methods I give could be made robust enough for use in
court. I assume that IPA is isopropyl alcohol?

Starch should be positive with the periodic acid Schiff method (PAS) and
may be 'confirmed' as such by using an amylase control. Starch should also
show 'Maltese Cross' birefringence under polarised light. The cellulose
should also be PAS positive, resist amylase and show 'linear' birefringence.

The protein is a little more difficult to confirm absolutely but if these
are skin cells they will contain cytokeratins - thus giving the possibility
of using immunocytochemistry with a pancytokeratin antibody.

Good luck!

PS Why is an IR/Raman engineer doing forensic work on paper/cells?

At 16:07 2001-02-28 -0800, HAMMOND,LOMA (HP-Corvallis,ex1) wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best regards

Gareth

'Coelum non animum mutant qui trans mare currunt'

e-mail Gareth.Morgan-at-impi.ki.se
http://www.ki.se/biomedlab

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu Mar 1 03:36:46 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wednesday, February 28, 2001 3:52 PM
Subject: Fwd: SEM of fungus

Contents Retrieved from Microscopy Listserver Archives
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Congo red will stain cellulose red, and can also be viewed by
fluorescence using a green (RITC-type) excitation filter. Cellulose
and starch are also birefringent, which you could demonstrate by using
crossed polarizers. A 1/4 wave or 1-wave plate may make this easier to
see. The fluorescence of Cellulose stained with congo red is also
polarized because the CR molecules line up on the oriented cellulose
microfibrils. As has already been mentioned starch and cellulose stain
with periodate-Schiff. If you have access to a fluorescence
microsocope you might also try a fluorescence variant of PAS, by
replacing schiff reagent with Lucifer Yellow CH. View with FITC-type
excitation.

Iodine/potassium iodide will stain starch blue/black.

Chris

----- Original Message -----
} From: "HAMMOND,LOMA (HP-Corvallis,ex1)" {loma_hammond-at-hp.com}
To: "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 01, 2001 12:07 AM

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Fungal people,
Although vapor fixation works sometimes and immersion fixation also works sometimes, often fungi samples are too delicate to handle with routine techniques. Just immersion in an aqueous solution can damage the structures. Repeated washings also can wash away delicate conidia. CPD causes shrinkage which may not be a major concern with some samples but can be a real problem with delicate ones like fungal hyphae.
Really the very best way to deal with many fungi samples is cryo SEM. True you may have to coat a bit longer than normal, etc. but the structures, when instantly frozen, are most likely to be similar to the living state. Unfortunately many people do not have access to Cryo -SEM equipment so must settle for the next best approach....fixation and drying by one of the methods already mentioned.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907

--------------------------------------


Microlisters,

While the simpler and less time consuming suggestions already mentioned in
this thread may work in some cases, I found out a long time ago that the
only way to deal with the "fungal jungle", eg. fungi cultured on agar
plates, is to hit 'em with the ol' OTO method, preceded by glutaraldehye
fixation. Quick outline is as follows:

Glut. fixation, your favorite buffer
Buffer rinses
Osmium tetroxide, 2%
Water rinses
Thiocarbohydrazide, sat. soln.,filtered
water rinses
Osmium tetroxide, 2%
Water rinses
dehydration series, EtOH, or acetone
CPD
metal coating? Maybe, maybe not. Try without first.

This eliminates the charging, and fixation is quite good.

Classic reference for this method:

"Ligand-mediated osmium binding:Its application in coating biological
specimens for scanning electron microscopy". Kelley, Robert O., A.F. Dekker
and John G. Bluemink. J. Ultrastrucute Research 45:254-258 (1973).

See also:

"Non-coating techniques to render biological specimens conductive/1980
update". Judith A. Murphy. Scanning Electron Microscopy/1980 Vol.I, p.
209-220.


Good luck!

Gib Ahlstrand

-----------------------------------------------------------------
Rad0

You might try exposing your non-aquatic sample (bread molds, mushrooms,
etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4%
OsO4 on the lid of a very small container with your sample inside or the
lid over the sample directly), then air dry, mount, coat and view. As with
any sample, the drying artifacts vary with the fungus. Some things look
great.

good luck

Steve
} -----------------------------------------------------------------
} Hello,
}
} Forgive me, I haven't tried to find this on my own yet...
}
} But have any of you used an electron microscope to get a
} close-up of a fungus? Can this be done?
}
} Thanks...



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/






From daemon Thu Mar 1 08:07:17 2001



From: Bob Bagnell :      rml-at-grayhawk.med.unc.edu
Date: Thu, 1 Mar 2001 09:04:12 -0400
Subject: Phase Contrast Artifacts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As others have pointed out, phase halo is easily demonstrated.
Shading-off is a different matter, at least for me. Both artifacts are the
result of incomplete segregation of the direct and diffracted light to the
conjugate and complementary areas respectively of the phase plate.
Shading-off is described in "Advanced Light Microscopy" vol. 2 pg. 15-20 by
Maksymilian Pluta. Shading-off appears as a brighter central area becoming
darker toward the edge in positive phase contrast, and just the reverse for
negative phase contrast. Pluta has illustrations of this on pages 18 and
19, but he doesn't say what the object is. It looks like a crystal of some
kind.

My point in bringing this up is to solicit others ideas on how best
to teach students (as well as ourselves) to interpret the phase contrast
image. The phase image has these artifacts superimposed on it. The phase
halo can mislead a novice but actually help an expert. Shading-off is more
subtle in a complex specimen than it is in Pluta's example, and I have
never seriously considered how it affects the phase image. I wonder if
there is a biological sample that clearly shows both artifacts? Any
thoughts and suggestions on this?

Bob

_____________________________________________________________
C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof.
Microscopy Services Laboratory
Department of Pathology & Laboratory Medicine
CB #7525 UNC-CH, Chapel Hill, N.C. 27599
ph 919-966-2413 fx 919-966-6718
http://www.pathology.med.unc.edu/path/microscopy/welcome.htm




From daemon Thu Mar 1 08:50:20 2001



From: kuznetsv-at-geo.komisc.ru
Date: Thu, 1 Mar 2001 08:48:53 -0600
Subject: Ask-A-Microscopist: Stereo Graphic Projections

Contents Retrieved from Microscopy Listserver Archives
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---------------------------------------------------------------------------

Email: kuznetsv-at-geo.komisc.ru
Name: Chuprov Georgy
School: The Institute of Geology
State: Republic of Komi

Question: Please, tell me.
Where I can find a computer program for plot of
stereografic projections of optical axes
(were tested by optical microscop)?

---------------------------------------------------------------------------




From daemon Thu Mar 1 09:32:28 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 1 Mar 2001 09:28:12 -0600
Subject: TEM: Focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have one of those very basic "thought" questions on a procedure that I've
always taken for granted, but am now not so sure about. I had been trained
from Day One to always focus a TEM with the beam at crossover, then to
spread the beam until the exposure intensity is reached. The idea seems to
be that small focusing errors at crossover will be corrected as the beam is
spread and "depth of focus" is increased somehow.

The reason I'm asking is that in recent years, almost no one else I've run
across focuses in this way, preferring instead to focus with the beam
already spread to the point at which the photograph will be taken (at least
as long as the image is bright enough to see for focusing). Out of
curiosity, I ran tests using both methods and have noted no clear
differences.

Does anyone have any thoughts on this? Any ideas or theories about why one
method would be superior to the other?

Sincerely,
Curious in Columbia

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Mar 1 10:03:21 2001



From: Bruce Ingber :      bingber-at-srrc.ars.usda.gov
Date: Thu, 01 Mar 2001 09:56:05 -0600
Subject: SEM of fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Ahlstrand's technique is excellent for imaging hyphal structures and some spore bodies while the stick on method of Dr. Mary Mager works well for certain individual spores. However, imaging of fruiting body structures (conidiophores, etc.) are often best prepared by using Osmium vapor fixation followed simply by air-drying (AD). One can also add minimal solvent exchange before AD, but some structural collapse will be observed in any event. "One size does not fit all" for fungi, sometimes even within one species.

We have a new ESEM now and hopefully combinations of low vacuum technology and various wet modes will improve imaging for our mycologists.


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Microlisters,

While the simpler and less time consuming suggestions already mentioned in
this thread may work in some cases, I found out a long time ago that the
only way to deal with the "fungal jungle", eg. fungi cultured on agar
plates, is to hit 'em with the ol' OTO method, preceded by glutaraldehye
fixation. Quick outline is as follows:

Glut. fixation, your favorite buffer
Buffer rinses
Osmium tetroxide, 2%
Water rinses
Thiocarbohydrazide, sat. soln.,filtered
water rinses
Osmium tetroxide, 2%
Water rinses
dehydration series, EtOH, or acetone
CPD
metal coating? Maybe, maybe not. Try without first.

This eliminates the charging, and fixation is quite good.

Classic reference for this method:

"Ligand-mediated osmium binding:Its application in coating biological
specimens for scanning electron microscopy". Kelley, Robert O., A.F. Dekker
and John G. Bluemink. J. Ultrastructure Research 45:254-258 (1973).

See also:

"Non-coating techniques to render biological specimens conductive/1980
update". Judith A. Murphy. Scanning Electron Microscopy/1980 Vol.I, p.
209-220.


Good luck!

Gib Ahlstrand

-----------------------------------------------------------------
Rad0

You might try exposing your non-aquatic sample (bread molds, mushrooms,
etc. ) to vapors of Osmium tetroxide for several hours ( a few drops of 4%
OsO4 on the lid of a very small container with your sample inside or the
lid over the sample directly), then air dry, mount, coat and view. As with
any sample, the drying artifacts vary with the fungus. Some things look
great.

good luck

Steve
-----------------------------------------------------------------

Dear rad0,
I have looked at fungus and fungus spores on my SEM and they are very simple
to prepare. Just stick them to a stub by sticky tab or glue and then gold
coat. They are very robust and they don't need any fixing or dehydration.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca

} -----------------------------------------------------------------
} Hello,
}
} Forgive me, I haven't tried to find this on my own yet...
}
} But have any of you used an electron microscope to get a
} close-up of a fungus? Can this be done?
}
} Thanks...



___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/


Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
bingber-at-srrc.ars.usda.gov
504-286-4270 phone
504-286-4419 fax



From daemon Thu Mar 1 10:39:36 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 1 Mar 2001 16:46:09 +0000 (GMT Standard Time)
Subject: Re: Quantitative stereo TEM (quite long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rod,

You might guess from the lack of response that quantitative
stereoscopy using TEM is not that popular a pastime. I was
involved some 20 years ago and can offer some comments
which were relevant then.

There is a big difference between TEM and SEM stereo
analysis. In SEM images you have a lower surface that can
be seen easily, in TEM images you have a view through both
surfaces and the specimen, the surfaces are very hard to
distinguish. It is relatively easy to calculate the height
from a fixed surface as in a SEM micrograph. Calculating
the height from an 'invisible' surface, as in a TEM
micrograph, is very difficult. It's OK to think of
something like a dislocation running through the specimen
and then assume that it ends at either surface but very few
examples go through from surface to surface and thus are
considerably more difficult.

Height measurements are made from stereo pairs by measuring
the parallax between the two tilted images. Provided the
tilt axis is known and the magnifications corrected, for
any change in focus current, then the height difference
between two points can be calculated. This is a long way
from reconstructing a 3D image.

It is relatively easy to view stereo images using a stereo
viewer but not everyone can do it. You need two eyes of
equal strength and a certain type of brain. From memory the
mapmakers said 50% of the population could see stereo
properly and 10% could make accurate measurements.

There were programs that would allow 2 stereo micrographs
to be placed side by side on a graphics tablet and then the
same points marked on both micrographs. The height
differences between these points were then calculated using
the first pair of points as a reference height. I don't
know if these are still available for modern computers
(they ran on an Apple 11+).

Stereo viewers are available that will project a 'floating'
point of light in the specimen that you can position onto a
feature and then read out the height from a micrometer (it
will need to be scaled to get the real height). We modified
one of these viewers to record and calculate these heights
(and X,Y coordinates) automatically), but it it still a lot
of work to produce even a simple 3D image.

I am not aware of any image recognition program that will
analyse your two images and produce a 3D image from them.
(Well if that doesn't get details sent in nothing will!)

If you want some references then in the mid 70s Hudson and
Makin descibed the how to select the tilt angle taking into
account the film thickness and the final magnification.
Much of the work at that time was being done by Alan Boyde.
I may be more use with references when I get my office back
- contact me off list in a few days if you want 20-25 year
old refences.

Good luck,
Ron


On Wed, 28 Feb 2001 10:03:56 -0700 Rodney McCabe
{rmccabe-at-lanl.gov} wrote:

}
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}
-----------------------------------------------------------------------.
}
} } Dear Colleagues:
} } I am interested in doing quantitative stereo microscopy
using a TEM and am } looking for software that may be
helpful in such an endeavor. I anticipate } the primary
application will be examining the spatial arrangement of
linear } defects. I have not been able to find a software
designed directly for } quantitative stereo TEM. I have
come across one commercial software that } can be used for
SEM surface topography that may be applicable. It seems }
like there must be other software packages out there used
for applications } such as surface mapping, electron
tomography, or even astronomy that could } be useful.
Also, as I am new to stereo microscopy, any words of wisdom
} would be appreciated. }
} Thanks, }
} Rod }
} }

----------------------
Mr. R.C. Doole Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Mar 1 11:12:48 2001



From: Pete Augustus +44 1327 356362 :      pete.augustus-at-marconi.com
Date: Thu, 01 Mar 2001 17:07:55 +0000 (GMT)
Subject: Re: History of TEM trivia - dislocations

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Pete Augustus
Caswell Technology


Hi Listers,

I'm preparing a talk which is to include a timeline giving dates and names
for some relevant TEM developments. I'm trying to find answers to two
trivia questions with great importance to microscopy:

1) Who was the first to knowingly image a dislocation in a TEM, when was it
done and where was it published? On this, I have found references as far
back as an article by Heidenreich in J. Appl. Phys, 1949, but don't have
this journal going back that far.

2) Who developed the solid state detector for energy analysis of x-rays
(EDS), when and where published?

I have found some references to books on TEM history by Marton (1968) and
Hawkes (1985) but don't have these on hand.

Thanks,

Wharton

***********************
Wharton Sinkler
UOP LLC
Des Plaines, IL




From daemon Thu Mar 1 12:05:36 2001



From: Edward J. King :      king-at-biology.utah.edu
Date: Thu, 01 Mar 2001 11:04:37 -0700
Subject: Re: SEM of fungi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For fragile conidiophores and their associated conidial chains, it is
still possible to use critical point drying after vapor fixation with
OsO4.

A fairly simple dehydration technique that avoids the solution changes
-- and turbulence -- that often lead to loss of the conidia from aerial
conidiophores (and the air drying that often does the same), is
described at Can. J. Microbiol. 29:653-658 (1983).

It's an adaptation of a slick idea published in Naturwissenshaften
17:402-403, for TEM, in 1962(!).

Ed King


From daemon Thu Mar 1 14:33:03 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 01 Mar 2001 15:30:29 -0500
Subject: RE: Focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I always "heard" to focus the image with the beam in an overfocus condition
(past crossover) for improved beam coherence. I find it difficult to focus at
crossover anyway. Also, I don't think you can accurately assess focus or
astigmatism with the beam at intensities suitable for most photography.
Depends on the magnification and a host of other things, of course, such as
your exposure time. I don't think there's any justification to focus at
exactly the same intensity at which you will photograph. I too await further
enlightenment from the list....

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Thursday, March 01, 2001 10:28 AM, Tindall, Randy D.
[SMTP:TindallR-at-missouri.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
}
} I have one of those very basic "thought" questions on a procedure that I've
} always taken for granted, but am now not so sure about. I had been trained
} from Day One to always focus a TEM with the beam at crossover, then to
} spread the beam until the exposure intensity is reached. The idea seems to
} be that small focusing errors at crossover will be corrected as the beam is
} spread and "depth of focus" is increased somehow.
}
} The reason I'm asking is that in recent years, almost no one else I've run
} across focuses in this way, preferring instead to focus with the beam
} already spread to the point at which the photograph will be taken (at least
} as long as the image is bright enough to see for focusing). Out of
} curiosity, I ran tests using both methods and have noted no clear
} differences.
}
} Does anyone have any thoughts on this? Any ideas or theories about why one
} method would be superior to the other?
}
} Sincerely,
} Curious in Columbia
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}



From daemon Thu Mar 1 15:06:41 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 1 Mar 2001 16:03:13 -0500
Subject: position opennings

Contents Retrieved from Microscopy Listserver Archives
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ADVERTISEMENT

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fax (407) 275-4321
--------------------------------------------------------------------
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Experience comes from making bad judgement."

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********************************************************************




From daemon Thu Mar 1 17:04:56 2001



From: William P. Sharp :      wsharp-at-asu.edu
Date: Thu, 01 Mar 2001 15:59:52 -0700
Subject: Focusing

Contents Retrieved from Microscopy Listserver Archives
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I have focused at a higher beam intensity than I use for exposing the
micrograph for many years, and, in fact continue to do so on our Philips
EM201. When we got a new Philips TwinLens CM12S in December of 1988, we
started to have problems with slightly out of focus micrographs across the
board - all experienced biological microscopists - about 10-12 of us - had
the same problem. Philips, to their credit, tried repeatedly to find and
solve the problem. They sent high level engineers, applications experts,
attached a high mag TV camera, changed all of the high tension cabling,
insulator, wehnelt, column liner, etc. over the course of nearly a year.
Towards the end of 1989, in desperation and because the biological TEM
applications experts were all busy, a materials science applications
engineer came to the lab and made beautiful, perfectly focused micrographs.
We could not duplicate his performance. Finally, he asked to watch one of
our most experienced and well published microscopists work. When the
Professor increased beam intensity to focus and stigmate, the applications
engineer went ballistic! he maintained that the focus MUST be done at the
intensity at which the micrograph is made. We did argue, but also agreed to
test the hypothesis. He was, of course, correct (IN THIS PARTICULAR CASE).
As we later found out, changing the C2 lens current also has a subtle
effect on the objective lens current, changing focus enough to cause
problems. Our service engineer was later able to demonstrate the effect
(although it doesn't show up when monitoring the lens current page) and
this strange phenomenon was written off to a design compromise which,
apparently, physicists and materials scientists are used to and expect, but
which biologists and cell biologists had never heard of. Something to do
with short focal length objective lenses, low contrast which makes focusing
more difficult, and compromises required to allow STEM and TEM to co-exist
in the same column and be switched back and forth with a minimum of
re-alignment. Anyone else have this experience??
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Thu Mar 1 18:54:11 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 01 Mar 2001 16:53:17 -0800
Subject: RE: Focusing

Contents Retrieved from Microscopy Listserver Archives
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I spread the beam as much as possible in order to be able to see the
changes during the focusing (usually the intensity is less than necessary
to make a photograph, so, I have to condense the beam a little bit for
photographing). I am using "three-chick" technique: rotating focus-control
knob in both directions to find the position when you will clearly see the
transition: "owerfocus-infocus-underfocus" on the background's
granules. This technique works for me from x40K and up. At the lower
magnification, I am using wobbler to focus (there is a "blind spot" at
x30-40K). The "three-chick" technique is a good test how your instrument is
aligned. The number of "clicks" depends from current setup and model of
coarse. I simply could not focus correctly if the beam is condensed too much.

Sergey

At 03:30 PM 3/1/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Mar 1 20:46:33 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 1 Mar 2001 20:41:43 -0600
Subject: Re: Focusing

Contents Retrieved from Microscopy Listserver Archives
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Hi again,

Well, I got my answer----right from one of the people who initially trained
me back in the '80's (and, in fact, is responsible for me being in the field
in the first place----that'll teach him).

Here is John Bozzola's reply to my post on focusing TEM's at beam crossover
versus a defocused beam: "(T)his is a holdover from (our Hitachi) HU11A when
illumination was a problem at the higher mags and when no focus wobbler was
available for low mag work. On the other hand, it still DOES work for
individuals who have diminishing eyesight and need the extra illumination
and minimal depth of field. Unfortunately, it greatly increases the
possibility of specimen damage and should be used only on hardy specimens.

"The bottom line is that if you have normal (or acute) vision, you won't
need to do this in modern TEMs. Of course, I sometimes still "check" my
focus by doing this..... old habit, I guess."

Now this is very interesting to me, because the first TEM I ever used was
the Hitachi HU11AB. I was trained to focus carefully at crossover, then
defocus until the proper exposure intensity was reached. Somehow over the
years, I ended up with the notion that there was a property of electron
"optics" that made this the preferred method of focusing because it
minimized focusing errors by increasing the depth of focus. I really don't
recall where this notion came from, but I trained dozens of students and
others in this way of focusing. Now I wonder how many others were trained
by them to do the same thing and will be just as stubborn about it as I have
been.

Probably not much harm done, really, but this reminds me of one of my
favorite stories about a woman cooking a ham for a family reunion picnic.
Her daughter watched her mother cut off the end of the ham before putting it
in a pan in the oven, and she asked why she did that. Her mother responded
that that's how she was taught by HER mother, but she didn't really know
why. She became curious, and at the reunion she asked her mother why she
had always cut the ends off hams before cooking them. The response was that
she had also been taught that way by her mother. Both curious now, they
hunted up Great-grandmother at the picnic and asked her why she had cooked
hams with the ends cut off. Great-grandmother answered, "I only had one pan
and it was too short for the ham."

Lesson learned. Again.

Cheers,
Randy

Randy Tindall
Electron Microscopy Core
University of Missouri




From daemon Thu Mar 1 21:20:51 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Thu, 1 Mar 2001 15:30:22 -0600
Subject: RE: Focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In terms of contrast transfer theory (i.e. when linear imaging allows
approximating the imaging process as convolution with a point spread
function), the lens transfer functon doesn't depend on convergence. So for
example you shouldn't effect the defocus of the image by slight changes of
the illumination convergence.

However, the convergence imposes a damping on the total contrast transfer,
which gets more severe when convergence is greater. For details on this
'spatial coherence envelope' see any text on HREM. Because of this, the
image quality will improve if you record with a less condensed beam (better
coherence).

With a field emission gun, you won't be able to image at crossover, because
the source will almost certainly be too small - these guns give the most
coherent illumination available.

For a thermionic gun, the limiting factor will be: if the convergence is too
great at crossover, you won't see much because everything will be blurred
out by the coherence limitation of the incident irradiation. If this is the
case, you should either change condenser apertures to decrease convergence
(then you can focus, and/or stigmate at crossover), or spread the beam to
improve coherence.

As far as I can see, there is no reason why one should or should not adjust
or even record images at crossover (except that if the filament is a bit
undersaturated, you will get uneven illumination). Getting the best
possible coherence is important, but it doesn't really matter how one
achieves this optically. Decreasing intensity is therefore good, to the
extent that sample drift doesn't become too much a limiting factor.

Overfocusing the illumination rather than underfocusing is better, I would
say (based on experience). I'm not sure why - perhaps some of the energy
spread gets filtered out more effectively - at any rate for the same size
beam one has more intensity underfocused than overfocused, so something is
being cut out by overfocusing.

Wharton


} -----Original Message-----
} From: Matthew Lynn [SMTP:mlynn-at-miami.edu]
} Sent: Thursday, March 01, 2001 2:30 PM
} To: 'Microscopy-at-MSA.Microscopy.com'
} Subject: RE: Focusing
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I always "heard" to focus the image with the beam in an overfocus
} condition
} (past crossover) for improved beam coherence. I find it difficult to
} focus at
} crossover anyway. Also, I don't think you can accurately assess focus or
} astigmatism with the beam at intensities suitable for most photography.
} Depends on the magnification and a host of other things, of course, such
} as
} your exposure time. I don't think there's any justification to focus at
} exactly the same intensity at which you will photograph. I too await
} further
} enlightenment from the list....
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} (305)284-4736
} mlynn-at-miami.edu
}
}
} On Thursday, March 01, 2001 10:28 AM, Tindall, Randy D.
} [SMTP:TindallR-at-missouri.edu] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi all,
} }
} } I have one of those very basic "thought" questions on a procedure that
} I've
} } always taken for granted, but am now not so sure about. I had been
} trained
} } from Day One to always focus a TEM with the beam at crossover, then to
} } spread the beam until the exposure intensity is reached. The idea seems
} to
} } be that small focusing errors at crossover will be corrected as the beam
} is
} } spread and "depth of focus" is increased somehow.
} }
} } The reason I'm asking is that in recent years, almost no one else I've
} run
} } across focuses in this way, preferring instead to focus with the beam
} } already spread to the point at which the photograph will be taken (at
} least
} } as long as the image is bright enough to see for focusing). Out of
} } curiosity, I ran tests using both methods and have noted no clear
} } differences.
} }
} } Does anyone have any thoughts on this? Any ideas or theories about why
} one
} } method would be superior to the other?
} }
} } Sincerely,
} } Curious in Columbia
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
} }
}


From daemon Fri Mar 2 00:36:08 2001



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Fri, 02 Mar 2001 08:28:51 +0200
Subject: Strain exsolution in Cu wire

Contents Retrieved from Microscopy Listserver Archives
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We're analysing Cu wire here for purposes better known to ourselves. We
are noticing, but not able to image interesting variations in the
concentration of impurities, which suggests to us, either that the
original material was inhomogeneous, or that drawing the wire has caused
some kind of exsolution process. Have any of you encountered smilar
things in metals? Refs?
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Fri Mar 2 03:14:02 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 2 Mar 2001 08:54:18 +0000 (GMT Standard Time)
Subject: Re: RE: Focusing

Contents Retrieved from Microscopy Listserver Archives
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Hi Matt and Wharton,

To add to Wharton's explanation the reason overfocus is
better than underfocus is that he has a strong objective lens
(as most are these days). Many years ago underfocus would
have been better.

What we are trying to achieve is parallel illumination not
convergent (or divergent). Modern TEMs have strong objective
lenses and use the prefield (field above the specimen) as
part of their probe forming optics thus if there is a
crossover above the specimen (overfocus)the prefield
converges the diverging rays from this to form a parallel
probe. In underfocus conditions the crossover is below the
specimen and the probe on the specimen is made more
convergent by the objective lens prefield.

If you have a Lorentz (low field for magnetic work) pole
piece or an old instrument (pre 1970ish) the objective lens
is weak and does not have this prefield effect so
underfocus is more parallel.

Regards,
Ron

On Thu, 1 Mar 2001 15:30:22 -0600 "Sinkler, Wharton"
{WSinkler-at-uop.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} In terms of contrast transfer theory (i.e. when linear imaging allows
} approximating the imaging process as convolution with a point spread
} function), the lens transfer functon doesn't depend on convergence. So for
} example you shouldn't effect the defocus of the image by slight changes of
} the illumination convergence.
}
} However, the convergence imposes a damping on the total contrast transfer,
} which gets more severe when convergence is greater. For details on this
} 'spatial coherence envelope' see any text on HREM. Because of this, the
} image quality will improve if you record with a less condensed beam (better
} coherence).
}
} With a field emission gun, you won't be able to image at crossover, because
} the source will almost certainly be too small - these guns give the most
} coherent illumination available.
}
} For a thermionic gun, the limiting factor will be: if the convergence is too
} great at crossover, you won't see much because everything will be blurred
} out by the coherence limitation of the incident irradiation. If this is the
} case, you should either change condenser apertures to decrease convergence
} (then you can focus, and/or stigmate at crossover), or spread the beam to
} improve coherence.
}
} As far as I can see, there is no reason why one should or should not adjust
} or even record images at crossover (except that if the filament is a bit
} undersaturated, you will get uneven illumination). Getting the best
} possible coherence is important, but it doesn't really matter how one
} achieves this optically. Decreasing intensity is therefore good, to the
} extent that sample drift doesn't become too much a limiting factor.
}
} Overfocusing the illumination rather than underfocusing is better, I would
} say (based on experience). I'm not sure why - perhaps some of the energy
} spread gets filtered out more effectively - at any rate for the same size
} beam one has more intensity underfocused than overfocused, so something is
} being cut out by overfocusing.
}
} Wharton
}
}
} } -----Original Message-----
} } From: Matthew Lynn [SMTP:mlynn-at-miami.edu]
} } Sent: Thursday, March 01, 2001 2:30 PM
} } To: 'Microscopy-at-MSA.Microscopy.com'
} } Subject: RE: Focusing
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I always "heard" to focus the image with the beam in an overfocus
} } condition
} } (past crossover) for improved beam coherence. I find it difficult to
} } focus at
} } crossover anyway. Also, I don't think you can accurately assess focus or
} } astigmatism with the beam at intensities suitable for most photography.
} } Depends on the magnification and a host of other things, of course, such
} } as
} } your exposure time. I don't think there's any justification to focus at
} } exactly the same intensity at which you will photograph. I too await
} } further
} } enlightenment from the list....
} }
} } Matt
} }
} } Matthew J. Lynn
} } Center for Advanced Microscopy
} } University of Miami
} } (305)284-4736
} } mlynn-at-miami.edu
} }
} }
} } On Thursday, March 01, 2001 10:28 AM, Tindall, Randy D.
} } [SMTP:TindallR-at-missouri.edu] wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi all,
} } }
} } } I have one of those very basic "thought" questions on a procedure that
} } I've
} } } always taken for granted, but am now not so sure about. I had been
} } trained
} } } from Day One to always focus a TEM with the beam at crossover, then to
} } } spread the beam until the exposure intensity is reached. The idea seems
} } to
} } } be that small focusing errors at crossover will be corrected as the beam
} } is
} } } spread and "depth of focus" is increased somehow.
} } }
} } } The reason I'm asking is that in recent years, almost no one else I've
} } run
} } } across focuses in this way, preferring instead to focus with the beam
} } } already spread to the point at which the photograph will be taken (at
} } least
} } } as long as the image is bright enough to see for focusing). Out of
} } } curiosity, I ran tests using both methods and have noted no clear
} } } differences.
} } }
} } } Does anyone have any thoughts on this? Any ideas or theories about why
} } one
} } } method would be superior to the other?
} } }
} } } Sincerely,
} } } Curious in Columbia
} } }
} } } Randy Tindall
} } } EM Specialist
} } } Electron Microscopy Core Facility
} } } W122 Veterinary Medicine
} } } University of Missouri
} } } Columbia, MO 65211
} } } Tel: (573) 882-8304
} } } Fax: (573) 884-5414
} } } Email: tindallr-at-missouri.edu
} } } Web: http://www.biotech.missouri.edu/emc/
} } }
} } }
} }
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Mar 2 05:58:34 2001



From: Dmitry Cherny :      dtcherny-at-mpc186.mpibpc.gwdg.de
Date: Fri, 02 Mar 2001 12:52:33 +0100
Subject: Nanoscope III is needed

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

we are looking for a working Nanoscope III (III or IIIa) controller
together with the PC boards (Digital Instruments, Santa Barbara, USA). If
somebody is going to clean out its lab please contact us directly by phone,
fax or e.mail



With best regards,

Dr. Dmitry Cherny, PhD, Dr.Sc.

MPI for Biophysical Chemistry, Dept. of Molecular Biology
am Fassberg 11, D-37077 Gottingen, Germany
tel: +49(0) 551 201 1383; fax: +49(0) 551 201 1467
e.mail: dtcherny-at-mpc186.mpibpc.gwdg.de




From daemon Fri Mar 2 07:24:10 2001



From: marian miller :      millermn-at-email.uc.edu
Date: Fri, 02 Mar 2001 08:18:35 -0500
Subject: hydronephrosis

Contents Retrieved from Microscopy Listserver Archives
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does anyone have any suggestions on how to assess hydronephrosis with
paraffin sections of kidney using a quantitative technique. equipment
we currently use for other tissues include microscope, spot camera,
digitizing tablet, camera lucida, SigmaScan, and image processing
software plugins for adobe photoshop,


thanks so much, marian miller



From daemon Fri Mar 2 09:56:20 2001



From: FARNHAM, WARREN H :      WHFARN-at-solutia.com
Date: Fri, 2 Mar 2001 10:39:00 -0500
Subject: AFM Capabilities

Contents Retrieved from Microscopy Listserver Archives
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I have several questions for an AFM microscopist;

1. Can AFM really measure surface modulus?

2. If yes, what is the mode of operation or measurement?

3. Is it a quantitative measurement? Is it an absolute measurement
or a relative measurement?

4. Can the results be correlated to the (bulk) modulus measured by
rheometer?

Regard;
Warren


From daemon Fri Mar 2 10:01:07 2001



From: Arrowood, Roy :      arrowood-at-utep.edu
Date: Fri, 2 Mar 2001 08:59:05 -0700
Subject: Reply: strain exsolution in copper wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nonuniform distribution of impurities in metal wires is not uncommon. There
may be a core-to-surface variation, which may be caused by contamination (or
de-contamination) of the surface layer during drawing or annealing .
Variations in impurity concentration may also relate to original
inhomogeneity in the billet prior to drawing. Segregation of impurities to
dislocation-rich deformed areas is also a possibility.

What are the impurity elements that you are seeing? How are the
concentration variations distributed spatially? (Radial gradients,
irregular patches smaller than wire diameter or larger than wire diameter,
wire bends vs. straight segments, or what?)

-------Roy
====================================
Roy Arrowood, Associate Professor
Metallurgical and Materials Engineering
UTEP, El Paso, TX 79968-0520
(915)747-6934
arrowood-at-utep.edu



From daemon Fri Mar 2 10:05:05 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 2 Mar 2001 08:56:34 -0700
Subject: RE: Focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From a practical standpoint, wouldn't it be best to focus using the same
conditions that are later used for taking the images, unless there are
intensity or other issues that prevent that?

A few years back, when I was doing hi-res TEM, we would carefully set the
conditions on the TV screen, then turn off the room fan, move away from the
column, hold our breath and take a picture.

However, this may have been peculiar to hi-res materials work, where even
tiny changes in focus can have dramatic effects.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Thursday, March 01, 2001 2:30 PM
To: 'mlynn-at-miami.edu'; 'Microscopy-at-MSA.Microscopy.com'



In terms of contrast transfer theory (i.e. when linear imaging allows
approximating the imaging process as convolution with a point spread
function), the lens transfer functon doesn't depend on convergence. So for
example you shouldn't effect the defocus of the image by slight changes of
the illumination convergence.

However, the convergence imposes a damping on the total contrast transfer,
which gets more severe when convergence is greater. For details on this
'spatial coherence envelope' see any text on HREM. Because of this, the
image quality will improve if you record with a less condensed beam (better
coherence).

With a field emission gun, you won't be able to image at crossover, because
the source will almost certainly be too small - these guns give the most
coherent illumination available.

For a thermionic gun, the limiting factor will be: if the convergence is too
great at crossover, you won't see much because everything will be blurred
out by the coherence limitation of the incident irradiation. If this is the
case, you should either change condenser apertures to decrease convergence
(then you can focus, and/or stigmate at crossover), or spread the beam to
improve coherence.

As far as I can see, there is no reason why one should or should not adjust
or even record images at crossover (except that if the filament is a bit
undersaturated, you will get uneven illumination). Getting the best
possible coherence is important, but it doesn't really matter how one
achieves this optically. Decreasing intensity is therefore good, to the
extent that sample drift doesn't become too much a limiting factor.

Overfocusing the illumination rather than underfocusing is better, I would
say (based on experience). I'm not sure why - perhaps some of the energy
spread gets filtered out more effectively - at any rate for the same size
beam one has more intensity underfocused than overfocused, so something is
being cut out by overfocusing.

Wharton


} -----Original Message-----
} From: Matthew Lynn [SMTP:mlynn-at-miami.edu]
} Sent: Thursday, March 01, 2001 2:30 PM
} To: 'Microscopy-at-MSA.Microscopy.com'
} Subject: RE: Focusing
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I always "heard" to focus the image with the beam in an overfocus
} condition
} (past crossover) for improved beam coherence. I find it difficult to
} focus at
} crossover anyway. Also, I don't think you can accurately assess focus or
} astigmatism with the beam at intensities suitable for most photography.
} Depends on the magnification and a host of other things, of course, such
} as
} your exposure time. I don't think there's any justification to focus at
} exactly the same intensity at which you will photograph. I too await
} further
} enlightenment from the list....
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} (305)284-4736
} mlynn-at-miami.edu
}
}
} On Thursday, March 01, 2001 10:28 AM, Tindall, Randy D.
} [SMTP:TindallR-at-missouri.edu] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi all,
} }
} } I have one of those very basic "thought" questions on a procedure that
} I've
} } always taken for granted, but am now not so sure about. I had been
} trained
} } from Day One to always focus a TEM with the beam at crossover, then to
} } spread the beam until the exposure intensity is reached. The idea seems
} to
} } be that small focusing errors at crossover will be corrected as the beam
} is
} } spread and "depth of focus" is increased somehow.
} }
} } The reason I'm asking is that in recent years, almost no one else I've
} run
} } across focuses in this way, preferring instead to focus with the beam
} } already spread to the point at which the photograph will be taken (at
} least
} } as long as the image is bright enough to see for focusing). Out of
} } curiosity, I ran tests using both methods and have noted no clear
} } differences.
} }
} } Does anyone have any thoughts on this? Any ideas or theories about why
} one
} } method would be superior to the other?
} }
} } Sincerely,
} } Curious in Columbia
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
} }
}


From daemon Fri Mar 2 10:38:47 2001



From: E.M.M. Manders :      e.manders-at-chem.uva.nl
Date: Fri, 02 Mar 2001 16:28:13 +0100
Subject: Final PROGRAM Focus on Microscopy 2001, Amsterdam, April 1-4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



***************************************************
PROGAM: FOCUS ON MICROSCOPY 2001
***************************************************

Dear colleagues,

The program of the conference "Focus on Microscopy 2001", April 1 - 4,
2001, Amsterdam, has been finalised and can be found on our Web-site

www.focusonmicroscopy.org

This conference is the 14th in a successful series of interdisciplinary
meetings on 3D acquisition and 3D image processing and forms an effective
meeting point for both developers and users of 3D-microscopy. The more than
120 contributions this year range from new developments in Coherent
Anti-Stokes Raman Microscopy (CARS) and multi-photon excitation to the
application of such techniques in biology, medicine and material sciences.
This year "3D Microscopy of living cells and tissues" will receive special
attention, which reflects the growing number of researchers that use
GFP-techniques and life-cell imaging for their experiments.

A substantial instrument exposition including the main manufacturers in the
field will accompany the conference.

Please, visit our web site http://www.focusonmicroscopy.org for further
details concerning the program and the conference.

Looking forward to seeing you in 1st of April in Amsterdam, on behalf of
the program committee,

Erik Manders and G.J. Brakenhoff


Conference Office:
------------------
Mrs M.P.A. Beunk-Timmers
Nicolaes Tulp Institute
PO-Box 23213
1100 DS Amsterdam
The Netherlands
Fax: +31-(0)20-6963228
Phone +31-(0)20-5668585
Web: http://www.FocusOnMicroscopy.org
E-mail: info-at-FocusOnMicroscopy.org





---------------
Erik Manders
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam

Visit: Kruislaan 316, Building III, room 2.07, Amsterdam, The Netherlands
Plantage Muidergracht 12, 4th floor, Amsterdam, The Netherlands
Mail: Kruislaan 316
1098 SM AMSTERDAM
The Netherlands
E-mail: e.manders-at-chem.uva.nl
Tel: +31-(0)20-5256225 (5257702)(5255136)
Fax: +31-(0)20-5256271
Web: http://wwwmc.bio.uva.nl/
http://www.FocusOnMicroscopy.org/
---------------



From daemon Fri Mar 2 10:47:46 2001



From: Marta Taules :      mtaules-at-medicina.ub.es
Date: Fri, 02 Mar 2001 17:50:50 +0100
Subject: LR White and gangliosides detection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

We are working with embbeded cells in LR White with and without osmium.
(Polimerization was acomplished at 60ºC). Our purpose is to detect
gangliosides (GD3). Do you have any suggestion?

Thanks a lot


Marta Taulés
Universitat de Barcelona
SCT





From daemon Fri Mar 2 11:22:53 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 2 Mar 2001 12:20:17 -0500
Subject: Re: Focusing

Contents Retrieved from Microscopy Listserver Archives
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I also was told early on in my career that one should focus at the
magnification the picture was to be taken at, though I find it difficult to
do this in practice because the illumination is often too low. I usually
compromise and focus with as little illumination as I can get away with.

I wonder whether the reason for the change in focus is not due to changes
in the illumination, but changes in the specimen position. At different
beam densities the degree of specimen charging may vary, thereby changing
the position of the specimen in the lens field. This might explain why
some microscopes are less sensitive to the specimen current density than
others. Certainly the amount of specimen charging seems to be affected by
the objective aperture, for example.

Marie


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Fri Mar 2 12:03:50 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 2 Mar 2001 10:00:15 -0800 (PST)
Subject: Re: TEM processing/formalin fixed tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peggy:
I don't know if you received any responses off-line, and I hope that Ronald
Austin was able to provide you with a protocol for his microwave procedure
(actually, if you have one available Ronald, I'd like a copy, please). Your
question is most appropriate because that is what I am (frantically) in the
midst of doing. Altho' "frantic" applies only to the deadline.... Sans a
microwave procedure, I am doing the old time-tested methods. The only thing
I make sure of is the buffer used to transfer the tissue from formalin. I
add 6% sucrose to the buffers and carry that through until I get into
osmium. Loss of membranes occurs in the formalin, and if you add the 6%
sucrose it seems to help retain membranes in the final EM sample.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.


On 26 Feb 2001 15:06:20 -0500 (EST),
"HARGER-ALLEN.MARGARET_J-at-INDIANAPOLIS.VA.GOV"-at-sparc5.microscopy.com wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Pathology lab. listers: are there any new/and/or superior techniques
| for processing previously formalin fixed tissue for TEM?
| Thanks, Peggy Harger-Allen
| |TAB|email:harger-allen-at-indianapolis.va.gov
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Fri Mar 2 13:45:24 2001



From: pjp6 :      pjp6-at-dana.ucc.nau.edu
Date: Fri, 02 Mar 2001 12:39:46 -0700
Subject: TEM prep for fecal pellets of Hydrobiid snail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The pellets are from a hydrobiid snail found in highly mineralized warm desert
springs and Posos in Mexico. I am interested primarily in the bacteria found
on
and in them but would also like to be able to inventory the entire contents of
the pellets to help get a better picture of what part they play in their
isolated ecosystem. I've done some light and SEM on samples but need to get
inside to determine if the bacteria that break them down are carried
internally or are picked up environmentally from the water. For sample
preparation and sectioning I need to consider other contents like diatoms and
stromatolite pieces and the high concentration of mineral solids in the
samples. To start I plan to fix with glut/OsO4, dehydrate with acetone,
infiltrate with propylene oxide and go into embed 812. After sections are
imaged for non organic I planned on staining with lead citrate and comparing.
Being new to TEM and not having a good idea of the precise osmolarity of the
sample has left me puzzling over buffer selection (sodium cacodylate vs.
phosphate), concentration, and times. We have an excellent EM technician here
at NAU but it seems more my responsibility to make these determinations so any
suggestions or references you could offer would be greatly appreciated.
Thanks
Pete Polsgrove
Northern Arizona University
pjp6-at-dana.nau.edu




From daemon Fri Mar 2 15:38:12 2001



From: Leonardo Lagoeiro :      lagoeiro-at-degeo.ufop.br
Date: Fri, 2 Mar 2001 18:35:22 -0300
Subject: Nikon CoolPix 990

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

we have just bought a Nikon CoolPix 990. It is an outstanding camera.
One thing that we are disapointed it is a focusing problem when
taking picture in Microscope. For any zoom setting and even in a
manual mode, the micrograph borders always get out of focus, and in
some situation with a Chromatic aberration.
Does anyone have any idea how to eliminate this problem? The camera
came with all necessary accessories for mounting in a microscope,
e.g., c-mount, and MDC relay lens and remote cable.


Best regards,

Leonardo
--
---
Leonardo Lagoeiro
Departamento de Geologia
Universidade Federal de Ouro Preto
Ouro Preto, MG, 35400-000
Brazil
E-mai: lagoeiro-at-degeo.ufop.br


From daemon Fri Mar 2 23:24:38 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 02 Mar 2001 21:20:20 -0800
Subject: Re: Nikon CoolPix 990

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The CoolPix 990 is indeed a nice camera. Unfortunately, it is
not ideal for microscopy. It will work in this type of application
but the results are mixed and the effort can be great.

If you zoom out to infinity, you should get best results. The
key problem I have found is obtaining consistent exposure
values. Preview with the puck looks good but the captured
image is over exposed. Trying the best of five sometimes
helps. But i have relegated the 990 to travel and snapshots.
I don't think that it is ready from prime time microscopy.
YMMV.

gary g.

http://photoweb.net



At 01:35 PM 3/2/01, you wrote:

} Dear All,
}
} we have just bought a Nikon CoolPix 990. It is an outstanding camera. One
} thing that we are disapointed it is a focusing problem when taking picture
} in Microscope. For any zoom setting and even in a manual mode, the
} micrograph borders always get out of focus, and in some situation with a
} Chromatic aberration.
} Does anyone have any idea how to eliminate this problem? The camera came
} with all necessary accessories for mounting in a microscope, e.g.,
} c-mount, and MDC relay lens and remote cable.
}
}
} Best regards,
}
} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br



From daemon Sat Mar 3 02:26:35 2001



From: awgrossm :      awgrossm-at-students.uiuc.edu
Date: Sat, 3 Mar 2001 02:28:13 -0600
Subject: 1um section staining for neurons vs. glia (LM/TEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am trying to distinguish between neurons and glia in
cortical tissue that has been embedded in epoxy resin
(LX112/NMA/DDSA/DMP-30). Does anyone know how to do
this on semithin sections? Toluidine-Blue staining
imparts some subtle differences in the appearance of
chromatin in the nuclei of neurons vs. glia, but i am
looking for a staining procedure that provides a more
obvious color difference (without going all the way to
immunohistochemistry).

It is my understanding also that KMnO4 will react with
NMA (even if i disolve the resin out??), so those
procedures are out, unless I can find a substitute for
KMnO4.

thank you in advance for your advice....

aaron grossman
univ. of illinois




From daemon Sat Mar 3 09:04:50 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 3 Mar 2001 07:54:18 -0700
Subject: Re: Nikon CoolPix 990

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

let me make two observations and see if you agree:

1) The Coolpix (and any other camera like it) is originally designed for
snapshots. That means, that the emphasis is on "nice" pictures under normal
(outside or flash) conditions. This does not necessary mean, that one can
take good scientific images. This may be the reason that you had problems
with exposure time. The camera may take a weighted measurement to calculate
exposure time, for example only from the middle. If you take a snapshot
outside, the most important feature is likely to be found somewhere around
the center of the image. So it's important to get that part right. That
might not apply to photography on a microscope.

2) The quality of the picture is affected by the weakest link in the chain.
My suspicion is, that the lens on the camera is far inferior to anything you
put on a microscope. For example, we spend a lot of time (and money) to
develop and make our own lenses for our TEM cameras. I don't see the point
of spending thousands of Dollars on good microscope lenses and then have all
that negated by an inferior lens on the camera.

One word to Nikon: I am not trying to knock this camera, it is probably a
very good camera (I don't know it myself). My remarks are general and apply
to other camera manufacturers (Kodak, Canon, Fuji, etc.). This was just a
thread which mentioned the Nikon camera. Plus, all these ramblings are my
personal opinion.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Friday, March 02, 2001 10:20 PM
To: Leonardo Lagoeiro
Cc: MSA listserver


The CoolPix 990 is indeed a nice camera. Unfortunately, it is
not ideal for microscopy. It will work in this type of application
but the results are mixed and the effort can be great.

If you zoom out to infinity, you should get best results. The
key problem I have found is obtaining consistent exposure
values. Preview with the puck looks good but the captured
image is over exposed. Trying the best of five sometimes
helps. But i have relegated the 990 to travel and snapshots.
I don't think that it is ready from prime time microscopy.
YMMV.

gary g.

http://photoweb.net



At 01:35 PM 3/2/01, you wrote:

} Dear All,
}
} we have just bought a Nikon CoolPix 990. It is an outstanding camera. One
} thing that we are disapointed it is a focusing problem when taking picture
} in Microscope. For any zoom setting and even in a manual mode, the
} micrograph borders always get out of focus, and in some situation with a
} Chromatic aberration.
} Does anyone have any idea how to eliminate this problem? The camera came
} with all necessary accessories for mounting in a microscope, e.g.,
} c-mount, and MDC relay lens and remote cable.
}
}
} Best regards,
}
} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br



From daemon Sat Mar 3 11:31:52 2001



From: Norman_C_Miller-at-raytheon.com
Date: Sat, 3 Mar 2001 11:27:02 -0600
Subject: GaAs cathodoluminescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

Does anyone have a GaAs cathodoluminescence attachment in a SEM? This means a
photomultiplier optimized to 8500A, an S1 photomultiplier. I would like to look
at light emitted from a GaAs diode under power. Or does anyone know of a lab
that does have GaAs cathodoluminescence as a SEM attachment.

Carl Miller
Raytheon Co./Lexington Lab




From daemon Sat Mar 3 12:46:08 2001



From: Xinran Liu :      xinran.liu-at-UTSouthwestern.edu
Date: Sat, 3 Mar 2001 12:44:58 -0600
Subject: Thank you / Vibratome section problem

Contents Retrieved from Microscopy Listserver Archives
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I would like to thank those colleagues who kindly provided their advice and
shared their experience with us regarding the problems of vibratome brain
sectioning that I posted on the list server a while ago.

We now use double-edge feather blades that we purchased from Ted Pella, the
quality of sections are much improved, also sometimes it seems helpful at
low amplitude rather than high one.

Xinran Liu, M.D., Ph.D.

Center for Basic Neuroscience
UT Southwestern Medical Center
6000 Harry Hines Blvd., NA4. 214A
Dallas, TX 75390-9111

Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu




From daemon Sat Mar 3 14:34:35 2001



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 3 Mar 2001 14:29:19 -0600
Subject: reply to 1um section staining for neurons vs. glia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Arron:
Try using Araldrite 502 for your resin. It does not contain NMA. This should
eliminate your concern with using KMnO4. The mixture I use is a 1-1
Araldrite 502 and DDSA, mix well then add your DMP-30 and mix again. Don't
worry about the air bubbles.

Ron Austin (Research Associate)
Dept of Pathology
LSU Medical Ct.
Shreveport, LA
rla-at-mindspring.com



From daemon Sat Mar 3 14:46:58 2001



From: pjp6-at-dana.nau.edu
Date: Sat, 3 Mar 2001 16:03:43 -0600
Subject: protocol for imaging fecal pellets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Original Message-----
} From: "PostMaster {rla-at-mindspring.com} "-at-sparc5.microscopy.com
[mailto:"PostMaster {rla-at-mindspring.com} "-at-sparc5.microscopy.com]
Sent: Saturday, March 03, 2001 2:43 PM
To: rla-at-mindspring.com




-----Original Message-----
} From: Ronald Austin [mailto:rla-at-mindspring.com]
Sent: Saturday, March 03, 2001 2:41 PM
To: Microscopy Society of America


---------------------------------------------------------------------------

Email: pjp6-at-dana.nau.edu
Name: Pete Polsgrove
School: Northern Arizona University


Question: I'm looking for an protocol for imaging fecal pellets. The
pellets are from a hydrobiid snail found in highly mineralized warm desert
springs and Posos in Mexico. I am interested primarily in the bacteria
found on and in them but would also like to be able to inventory the entire
contents of the pellets to help get a better picture of what part they play
in their isolated ecosystem. I've done some light and SEM on samples but
need to get inside to determine if the bacteria that break them down are
carried internally or are picked up environmentally from the water. For
sample preparation and sectioning I need to consider other contents like
diatoms and stromatolite pieces and the high concentration of mineral
solids in the samples. To start I plan to fix with glut/OsO4, dehydrate
with acetone,
infiltrate with propylene oxide and go into embed 812. After sections are
imaged for non organic I planned on staining with lead citrate and
comparing. Being new to TEM and not having a good idea of the precise
osmolarity of the sample has left me puzzling over buffer selection (sodium
cacodylate vs. phosphate), concentration, and times. We have an excellent
EM technician here at NAU but it seems more my responsibility to make these
determinations so any suggestions or references you could offer would be
greatly appreciated.
Thanks Pete Polsgrove

---------------------------------------------------------------------------




From daemon Sat Mar 3 18:31:27 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 03 Mar 2001 16:28:01 -0800
Subject: RE: Nikon CoolPix 990

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think you have hit the nail right on the head.

1) The camera is of course intended for general purpose
photography. Snapshots, indoors and outdoors. It has
a miniature flash which is good for about ten feet distance.
For microscopy, of course the flash is turned off.

2)There are some macro zoom situations where the camera
does a nice job. But for compound transmitted and reflected
work, I have not found it to be satisfactory. Optem has
made a high quality interface for the CoolPix which interfaces
to Zeiss, Olympus and many other 'scopes. I have a common
optical tube with C-mount to CoolPix for Axioskop and
Olympus. I cannot fault this adapter system at all. But
as you point out, the native CoolPix optics are not intended
for discriminatory microscopy work. At about $700 for
the Optem adapter, the CoolPix package with remote release
and AC adapter is not cheap.

I recently took 765 pix with the CoolPix on a trip to
Australia. It did a super job. I used two 128MB AVL
CF modules. Some images were in highest JPEG
resolution and some were in XGA fine. All print on
an Epson Stylus Photo 2000P with outstanding results.
And, of course, they go to the Web without any problem.

The Pixera Penguin 600CL is quite a different camera.
It is highly suitable for microscopy, but also will do
excellent macro work with an inexpensive C-mount
zoom lens. Optem also makes the adapter for this
camera to the Zeiss and Olympus microscopes.
Same high quality and fine results. I have done
multiple slices and stitched them together for a
final TIFF file size of 110MB.

The Pixera is non-interpolated 5.8M pixels using their
Diractor prism device. At 2776x2074 pixels, it generates
a 17.5MB TIFF file. In 16-bit mode, the size is twice that.
As I mentioned in a prior post, their software supports
multiple capture averaging and integration. It also does
variable sized spot metering and average metering. The CL version
has Peltier cooling and exhibits exceptionally low thermal
noise. Four frame averaging reduces noise even further.

The Pixera is 10-bits per color while the Nikon MX1200 is 8-bits
per color. The word on the street is that the next release
of the MX1200 will be 10-bits per color. Whether this is worth
anything to a particular user is their own decision.

Being a professional Nikon shooter for many years, I am
no longer an intransigent fan. In fact, I have dumped nearly
all of my Nikon SLR equipment in favor of Contax. This of course
has nothing to do with microscopy. But the point is that
in my extended experience, Nikon is struggling to develop new products,
which by specifications, leapfrog their competition--while in
practice, they do not.

Try before buy is a good operative in this respect. I really
don't care who makes what, as long as it is good stuff. Sorting
through all the marketing hype is a chore. Sometimes even
working with the equipment is a chore. But the actual results
of using products is much more telling than sales pitches or
product brochures.

gary g.

http://photoweb.net





At 06:54 AM 3/3/01, you wrote:

} Gary,
}
} let me make two observations and see if you agree:
}
} 1) The Coolpix (and any other camera like it) is originally designed for
} snapshots. That means, that the emphasis is on "nice" pictures under normal
} (outside or flash) conditions. This does not necessary mean, that one can
} take good scientific images. This may be the reason that you had problems
} with exposure time. The camera may take a weighted measurement to calculate
} exposure time, for example only from the middle. If you take a snapshot
} outside, the most important feature is likely to be found somewhere around
} the center of the image. So it's important to get that part right. That
} might not apply to photography on a microscope.
}
} 2) The quality of the picture is affected by the weakest link in the chain.
} My suspicion is, that the lens on the camera is far inferior to anything you
} put on a microscope. For example, we spend a lot of time (and money) to
} develop and make our own lenses for our TEM cameras. I don't see the point
} of spending thousands of Dollars on good microscope lenses and then have all
} that negated by an inferior lens on the camera.
}
} One word to Nikon: I am not trying to knock this camera, it is probably a
} very good camera (I don't know it myself). My remarks are general and apply
} to other camera manufacturers (Kodak, Canon, Fuji, etc.). This was just a
} thread which mentioned the Nikon camera. Plus, all these ramblings are my
} personal opinion.
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Friday, March 02, 2001 10:20 PM
} To: Leonardo Lagoeiro
} Cc: MSA listserver
} Subject: Re: Nikon CoolPix 990
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 4 05:58:36 2001



From: twilley :      jtwilley-at-sprynet.com
Date: Sun, 4 Mar 2001 05:54:31 -0600
Subject: SEM views of gold paint

Contents Retrieved from Microscopy Listserver Archives
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} }
} } Subject:
} }
} } SEM views of gold paint
} }
} } Content:
} }
} } Does anyone have any views of modern gold flake (real gold, not brass
} } flake) used in making paintable substitutes for gold leaf?
} }
} } John Twilley
} } Conservation Scientist
}




From daemon Mon Mar 5 02:00:05 2001



From: Nick SCHRYVERS :      schryver-at-ruca.ua.ac.be
Date: Mon, 05 Mar 2001 08:47:05 +0100
Subject: post-doc at Agfa

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VACANCY FOR POST-DOCTORAL RESEARCH SCIENTIST AT AGFA-BELGIUM

In a world in which nanostructured materials are of growing importance
for a variety of industrial applications, Agfa’s R&D laboratories are
increasingly oriented towards research on nanophased systems. In order
to decrease development time for new products based on functional
nanomaterials, a significant part of the research effort is spent on an
understanding of the basic working mechanisms in a variety of
nanostructured systems.

To reinforce this research-team, Agfa-Gevaert N.V. in Mortsel (Belgium)
has a 2-year vacant position for a postdoctoral scientist who will be
involved in an advanced characterisation programme of nanostructured
building blocks for new imaging and other functional materials,
including photo-, electro- and thermoresponsive systems. The objective
is to use advanced microscopical and analytical equipment to
characterise nanoscaled systems, with the aim of providing an increased
understanding in the relationship between nanostructuration and
properties. Moreover, this research work will also provide clues for a
controlled synthesis of nanostructured systems.

The candidate will be working in a diverse industrial R&D-environment
with several links to university labs. He/she will be involved in a
multidisciplinary team working on several nanostructured applications.
Main focus of the work will be on microscopical and crystallographic
(diffraction) techniques. In order to meet the technical requirements,
the candidate should have a PhD in materials science, experience with
TEM- and diffraction-techniques, and have a background in
crystallography of inorganic materials. He/she should be a good
teamplayer to function in a multi-disciplinary team, and be fluent in
English and/or Dutch.

Candidates please apply to the following persons :

Dr. Christiaan Van Roost
R&D Materials/Physics and Analytics
Septestraat 27
B-2640 Mortsel
Belgium
Phone : (32)03 444 37 00
E-mail : christiaan.vanroost.cv-at-belgium.agfa.com

Rene De Keyzer - Master in Applied Science
Manager External R&D - R&D Materials
Septestraat 27
B-2640 Mortsel
Phone : (32) 03 444 31 00
E-mail : rene.dekeyzer.rd-at-belgium.agfa.com

For selection procedures, eligibility criteria and so on, please see
homepage
Marie Curie Industry Host Fellowships: http://www.cordis.lu/improving.
Please note in particular that candidate fellows must be nationals of an
EU Member or Associated State, or have resided in the EU for at least
five years immdiately prior to their selection by Agfa.






From daemon Mon Mar 5 02:02:26 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Mon, 5 Mar 2001 09:02:55 +0100
Subject: Maintenance of LM; quest.

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Hi all

I don't want to wake up a recent discussion (I was away in the last
weeks), but I take advantage of this subject to askh a question about an
other aspect of this subject. Sometimes you have so old material and
maintenance services are laughing when they see what your are working with
and/or on the other hand (with new materials also) it's faster and cheaper
to do cleaning and (basic) maintenance yourself (and I like to do it
myself !).

My question :
What kind of grease/oil, where, on a LM ?

Manufacterer and maintenance services don't like to answer such a
question. They say its complicate, there are much type of greases etc
etc...
So, does someone know something about that.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Mon Mar 5 03:36:18 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Mon, 5 Mar 2001 04:30:54 -0500
Subject: TEM: Focusing

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Hi Randy

I was quite surprised to see the problems that are being discussed in
relation to TEM focus, particularly as I was with Hitachi in the HU11 days,
lets keep it simple and try to help?

When setting the image focus you should be aware of a number of problems
that may complicate matters.

1. To try to focus at condenser cross over is absolutely the wrong thing
to do!
i) At crossover there is the poorest illumination coherence,
this will lead to soft images, not out of focus but not optimised either.
ii) At crossover there is a space charge effect (too
many electrons in a small area and they repel each other) which may cause a
false focus. (Just look at any image at crossover and then overfocus the
condenser - you can see more detail!)
iii) To focus at crossover and then change to a lower
intensity is asking for trouble as the change in heating effect is likely
to cause specimen drift and flexing which may change the contrast of
material science specimens.

2. You MUST focus at the photographic magnification. Focus is the
matching of the focal lengths of the objective and diffraction lenses. If
you focus at a higher level and there is a diffraction lens change when
dropping the magnification, you will find a matching objective correction
will be required or your image will be out of focus.

3. What is best FOCUS, not always true focus? To a materials scientist
it will almost certainly be true focus, no Fresnel fringes, as determined
by the focus wobbler. They will set their intensity in advance as an
intensity change may cause the material to flex and change in contrast. To
the biological scientist it will be a degree of underfocus determined by
the specimen's organelle density, its thickness, the kV, the objective
aperture size and most important, the magnification; this is optimum under
focus, the defocus where the (white) Fresnel fringes enhance the high
contrast areas!

When taking a photograph the ideal conditions (as used in all the
manufacturer's demo laboratories that I have worked with, Hitachi, JEOL and
ISI) require the photograph to be taken at the same intensity level as when
the focus is set. WHY? Well, once an intensity is set the operator may
focus and during the focus procedure they should be concentrating
sufficient to spot any image drift; it is most important to see the image
under the conditions that you are going to photograph. So what will the
excuses be?

1. "It is too dim to focus" - then
i) increase the emission current, many people run at too low a
current and make the task of optimising the instrument settings much more
difficult
ii) increase the kV which would increase the intensity too
ii) re calibrate the photographic system to allow a focus intensity
that is suitable for most operators

2. "The negatives are to dark" -
i) the denser the negative the higher the contrast, so may be you
could increase the kV, have a more stable specimen and an even brighter
image?

3. Difficult one this - "the pathologists like a very bright image" - yes
I know I guess it comes from looking down light microscopes all day?
i) try 1 or 2 above - "yes but the pathologists like a high screen
contrast" - yes I know but you should explain that no one publishes the
screen, it it the photographic record that is the most important item(?)
and we can get stacks of contrast from modern printing/publishing systems.

Regarding the reported overlap in currents between condenser and objective
it is true, however the result is usually more of an image shift than a
focal change in my observations, but yet another reason to focus and
stigmate at the photographic intensity.

So to conclude I would

1. Always focus and stigmate at the photographic illumination level,
if a biologist determine and plot out the optimum under focus for your
material over your normal magnification range.
2. Always use the second condenser overfocus from crossover for high
coherence and combine this with a 2 micron spot size for biology.
3. Set up the instrument's photographic procedure to suit as many
operators as possible so that they too may focus at the photographic level.
4. Increase the emission current to give a higher brightness making
point 2 far easier to use
5. Always use a higher kV (if 60, wow please go to 80, if 80 try 100)
and work to obtain the best results on a photograph not trying to optimise
for the screen.
6. To focus at low magnifications try removing the objective aperture
and focus without a binocular, or a wobbler, looking for the very strong
minimum contrast effect.

Well I hope this helps, its standard information on our courses, guess we
do too much SEM in the States!

Regards

Steve Chapman
Senior Consultant Protrain
For consultancy and training by professionals World Wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com


From daemon Mon Mar 5 03:50:32 2001



From: Giles Sanders :      g.sanders-at-ic.ac.uk
Date: Mon, 5 Mar 2001 09:42:18 -0000
Subject: Re: AFM Capabilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I reply to Warren's question

}
} I have several questions for an AFM microscopist;
}
} 1. Can AFM really measure surface modulus?

Probably - but there are a number of factors which may have to be taken into
account. These will include the nature of sample, any additional
interactions between the sample and the scanning probe tip, the nature of
the tip and SPM cantilever and so on.

}
} 2. If yes, what is the mode of operation or measurement?
}
In terms of imaging modes (i.e. those where you are obtaining an image along
with surface property information) there are two that may provide some
surface modulus information.

i) Phase imaging (see for
e.g.http://www.di.com/AppNotes/Phase/PhaseMain.html) is capable of providing
a surface map based on the viscoelastic properties of the surface.
Therefore the image will probably be a convolution between both the surface
modulus and other surface properties (for example elasticity and whether the
sample has any interactions with the tip).
In this mode the probe is tapped into the surface and the lag between the
voltage driving the tip oscillation and the actual tip oscillation provides
this information. (It is particularly difficult, if not impossible to
quantise the results obtained).


ii) Pulsed force should be able to deconvolute between sample stiffness and
sample adhesion. http://www.thermomicro.com/tech/modes/pfm.htm should
provide more information about this mode. However whether true values could
be measured would again be a matter of some discussion.


Further mode of scanning probe microscopes are the spectroscopic ones in
which the probe tip is move towards and away from the sample and the tip
response measured. In AFM this mode is force-distance (see for e.g.
http://www.thermomicro.com/tech/modes/fvsd.htm).
This should again provide some information regarding the surface modulus.
And with some tip calibration may provide something akin to a quantitative
measurement.

With all of these mode the cantilever type will have an effect on the amount
of information available. If the cantilever is less stiff than the surface
of interest your measurement will generally be of the Young's modulus of the
cantilever.

Nanoindenters may provide a more accurate measurement.
http://freespace.virgin.net/micro.materials/ is an example. There are some
companies that offer methods of converting commercial SPMs towards a
nanoindenter.

} 3. Is it a quantitative measurement? Is it an absolute measurement
} or a relative measurement?

My cynical opinion would be that most measurements available from a more
qualitative than quantitative, though many would disagree.
For example in this case, the area of tip-sample contact is unlikely to be
able to be measured accurately but will have a major effect on your
measurements,
You may however be able to obtain some numbers that can be converted to an
absolute measurement after careful consideration.

}
} 4. Can the results be correlated to the (bulk) modulus measured by
} rheometer?


This would, I presume, depend upon the material.

I hope this is of some use.

Giles


----------------------------------------------------------------------------
---------------------------
Dr. Giles Sanders
Zeneca / SmithKline Beecham Centre for Analytical Sciences
Chemistry Department
Imperial College of Science, Technology and Medicine
London
SW7 2AY

Web: http://www.achem.ic.ac.uk/gsanders/web/giles.htm

Tel: (44) - 020-7594-5749
Fax: (44) -020-7594-5833



Never express yourself more clearly than you think.
-- Niels Bohr (1885-1962) Danish physicist

----------------------------------------------------------------------------
------------------------

}
} Regard;
} Warren
}
}



From daemon Mon Mar 5 09:59:37 2001



From: Colin Reid :      creid-at-truxa1.tcd.ie
Date: Mon, 5 Mar 2001 15:57:18 -0000
Subject: Remote Access to SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have had a request from a customer to allow remote access to our SEM in
operation ( Hitachi S3500N ). The software they are proposing to use is
"Timbuktoo" (?). They wish to set up three-way access across the internet
along with a web-cam.
I would appreciate advice from anyone who has tried this already. It would
be useful to know what software has been used elsewhere and what problems
have arisen. There may be better software available to carry out this
function, but I have not approached this problem until now and am a bit in
the dark.
All advice, comments, etc. will be much appreciated.

Thanks.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2,
Rep. of Ireland.
Tel: 353-1-6081820
Fax: 353-1-6770438
Email: creid-at-tcd.ie
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm




From daemon Mon Mar 5 10:52:29 2001



From: Anita Garg :      Anita.Garg-at-grc.nasa.gov
Date: Mon, 05 Mar 2001 11:47:45 -0500
Subject: Re: EMISPEC and other related systems

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues
We have a CM200 (S)TEM equipped with EDAX IIs, 4pi digital imaging system
and Gatan GIF. We are considering buying an integrated data acquision
system for this microscope. ES Vision System from Emispec Systems, Inc. is
under consideration . Comments from users of this ES Vision System would be
highly appreciated. Of particular interest is operation of the Gatan Image
Filter; would the autofilter functions still be available?
Are there any other such systems out there?
TIA
Anita



From daemon Mon Mar 5 12:01:22 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Mon, 5 Mar 2001 11:45:37 -0500
Subject: L.R. White

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To the person who is polymerizing the L.R. White at 60 degrees C:

Turn down the oven. If you want to do some sort of gold labeling on your
sections try polymerizing the blocks at 45 degrees C for 2 to four days. Be
sure to do this in some sort of BEEM capsule, as the stuff will not get hard
if it is exposed to air. This type of plastic is not very stable in the
electron beam, pick up your sections on carbon coated gold grids. Good
luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Mon Mar 5 12:25:13 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 05 Mar 2001 11:59:31 -0600
Subject: Re: Remote Access to SEM

Contents Retrieved from Microscopy Listserver Archives
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First off, I suppose that Nestor or the folks at ORNL will have much more
to say about this since they have essentially developed and refined
telemicroscopy. However, I have some experience with Timbuktu that I will
relate.

We have been using Tibuktu for a number of years since I first heard of it
several years ago at an MSA workshop on telemicroscopy. It is designed for
remote observation or control of computers in general and has been borrowed
for the microscopy application. There are several other products also
available for the same work. Norton has PC anywhere. ATT has developed VNC
which has been mentioned here before and is free.

Timbuktu (and the others) simply requires a connection to the Internet.
Whatever happens on the screen is potentially available to the world once
a client logs in with a password. This can slow down the host computer as
it also has to transmit a copy of the screen.

I don't know about PC Anywhere, but Timbuktu is much faster on the client
side than is WinVNC, even on a LAN. There can be a lot of data to transmit
and it flat out takes a while to do so. Well-written code can do a lot to
speed that up. You should also consider the connection between your PC and
your client. If they have only a modem connection, refresh rates will be
painfully slow.

I couldn't guarantee how well Timbuktu (or any other program) would work on
your scope. I seem to recall that there were some windows whose contents
were not available for Timbuktu to share. It may be that the SEM control
programs were doing non-standard operations that circumvented the normal
Windows system. Presently, I am able to view video windows whether they be
from Oxford's AutoBeam, Quartz's PCI or Dazzle's video preview. But don't
expect refresh rates measured in frames per second, at least not with a
remote control/viewer program. Still, it could be a good alternative
compared to more expensive solutions or to traveling to the site.

BTW, we also picked Timbuktu since it worked with either Macs or Windows
machines.

I cannot tell you much about web cams. I know some systems are available
that are stand-alone and practically plug and play. I don't know that I
would want to burden my microscopy or EDS computer with the extra task.
Others may have more to say.

There, that ought to be worth a couple of cents.

Warren

At 03:57 PM 3/5/2001 +0000, you wrote:
} Hi,
}
} I have had a request from a customer to allow remote access to our SEM in
} operation ( Hitachi S3500N ). The software they are proposing to use is
} "Timbuktoo" (?). They wish to set up three-way access across the internet
} along with a web-cam.
} I would appreciate advice from anyone who has tried this already. It would
} be useful to know what software has been used elsewhere and what problems
} have arisen. There may be better software available to carry out this
} function, but I have not approached this problem until now and am a bit in
} the dark.
} All advice, comments, etc. will be much appreciated.
}
} Thanks.
}
} Best wishes,
}
} Colin

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Mar 5 14:42:26 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 05 Mar 2001 12:28:44 -0800
Subject: Re: Remote Access to SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Timbuktu is a remote control software package made by
Netopia.

http://www.netopia.com/software/

There is a free trial version which can be downloaded.

Whether it will work for you, I don't know. One of the
key issues is real-time image transfer for mag and focusing.
The interconnect between the SEM and the remote user is
a major factor in response time. Slow analog modems
are not going to be all that responsive. If running on a
LAN or intranet, that would make a big difference.

A similar product is PC-Anywhere.

gary g.



At 07:57 AM 3/5/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 5 17:27:53 2001



From: ileyozerlat-at-yahoo.com ()
Date: Mon, 5 Mar 2001 17:25:50 -0600
Subject: Ask-A-Microscopist: Epiflorescent Microscope

Contents Retrieved from Microscopy Listserver Archives
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Email: ileyozerlat-at-yahoo.com
Name: Iley Ozerlat

Organization: Macalester College

Education: Undergraduate College

Location: St. Paul, Minnesota

Question: How does an "Epiflorescent Microscope" work?
What are the functions of the features of that
microscope?





From daemon Mon Mar 5 20:23:12 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 5 Mar 2001 21:01:49 -0500
Subject: RE: Remote Access to SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can do it very cheaply (i.e. free if you have Windows machines) using NetMeeting. Our LEO 1530 was easily set up to do this. You can do "over the shoulder" microscopy where the remote location observes. You need fast bandwidth for control and the instrument must be fully digitally controlled. We have tried it out with remote locations within our company and it works, but do not use it very much (i.e. never).


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Colin Reid [mailto:creid-at-truxa1.tcd.ie]
Sent: Monday, March 05, 2001 10:57 AM
To: MSA.Microscopy.Com (E-mail)
Subject: Remote Access to SEM


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of America
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Hi,

I have had a request from a customer to allow remote access to
our SEM in
operation ( Hitachi S3500N ). The software they are
proposing to use is
"Timbuktoo" (?). They wish to set up three-way access across
the internet
along with a web-cam.
I would appreciate advice from anyone who has tried this
already. It would
be useful to know what software has been used elsewhere and
what problems
have arisen. There may be better software available to carry out this
function, but I have not approached this problem until now and
am a bit in
the dark.
All advice, comments, etc. will be much appreciated.

Thanks.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2,
Rep. of Ireland.
Tel: 353-1-6081820
Fax: 353-1-6770438
Email: creid-at-tcd.ie
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm





From daemon Tue Mar 6 04:05:09 2001



From: Susanne Guder :      guder-at-wm.mw.tum.de
Date: Tue, 06 Mar 2001 09:48:21 +0100
Subject: Re:Strain exsolution in Cu wire

Contents Retrieved from Microscopy Listserver Archives
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Malcom,

to make a guess, you should can give some more information.
What impurity elements did you find/expect ?
Can you make any suggestion about the size of any kind of inhomogeneity -
conzentration, precipitation
or what ever ?

S.Guder


} We're analysing Cu wire here for purposes better known to ourselves. We
} are noticing, but not able to image interesting variations in the
} concentration of impurities, which suggests to us, either that the
} original material was inhomogeneous, or that drawing the wire has caused
} some kind of exsolution process. Have any of you encountered smilar
} things in metals? Refs?
} Malc.
}
} --
} Dr MP Roberts Phone: [+27](0)46 603 8313
} Dept of Geology Fax: [+27](0)46 622 9715
} Rhodes University Cell: 083 4060 262 (usually off)
} 6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
} SOUTH AFRICA

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr.-Ing. Susanne Guder
Technical University Munich
Department of Materials in Mechanical Engineering
D - 85747 Garching
Phone: + 49-(0)89-289-15308/15338
Fax: + 49-(0)89-289-15301
Email: guder-at-wm.mw.tum.de
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Tue Mar 6 04:16:16 2001



From: Alan E. Davis :      adavis-at-saipan.com
Date: Tue, 6 Mar 2001 20:11:18 +1000
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
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I own a zeiss 63X water immersion lens. If the coverslip thickness
is 0, does that mean that this is meant for direct viewing of
specimens in water? I purchased it with that in mind (I work in
marine environments, felt it would be convenient to observe
microorganisms and microanimals directly). Using a coverslip
doesn't seem to work well, if at all. For some specimens it is
nice, but any focusing movement will push specimens out of the way.
Nice for zooxanthellae in a small sea anemone, for example, and was
pretty good for a thick broth of unicellular green algae.

I am trying to get a feel for this objective. WOuld appreciate any
information possible.

Alan Davis
adavis-at-saipan.com


On Fri, 13 Oct 2000 08:51:07 -0400
Gary Radice {gradice-at-richmond.edu} wrote:

}
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} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} I've had some experience with water immersion lenses. Their main
} advantage is higher numerical aperture than dry lenses at
} equivalent
} magnification, so better theoretical resolution. They don't offer
} quite as good resolution as oil immersion lenses, but they tend to
} have longer working distances, I believe, and don't have the
} messy
} clean-up of oil immersion lenses. So, yes, they do have some
} distinct
} advantages.
}
} However, as others have pointed out, you may already be able to
} see
} everything you need to see with your current lenses. And nothing
} beats a test-drive.
} --
} Gary P. Radice gradice-at-richmond.edu
} Associate Professor of Biology 804 289 8107 (voice)
} University of Richmond 804 289 8233 (FAX)
} Richmond VA 23173 http://www.science.richmond.edu/~radice


--
adavis-at-saipan.com
1-670-235-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, NMI

I have steadily endeavored to keep my mind free, so as to give up
any
hypothesis, however much beloved -- and I cannot resist forming one
on
every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)






From daemon Tue Mar 6 04:17:50 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 06 Mar 2001 02:03:30 -0800
Subject: Philips XL30 models

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone priced the XL30 high vac FESEM
and the EFESEM or variations which use LaB6?
What is the general price range for these, and
which Hitachi models would closely compete?

Is service any good and is it reasonably priced?

All responses are welcomed.

tnx,
gary g.



From daemon Tue Mar 6 04:46:53 2001



From: Drouillon, Philippe :      Philippe.Drouillon-at-solvay.com
Date: Tue, 6 Mar 2001 08:42:13 -0600
Subject: Help wanted : looking for parts of a Siemens Elmiskop 102 TEM for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A normal (upright) epifluorescence microscope excites
fluorescence in a specimen by passing the excitation light to the
top of the specimen via the objective lens, which is used to focus
the light into an intense spot exactly at the point on the specimen
which is to be viewed. Fluoresced light from the region of interest is
collected by the objective and is viewed or photographed normally
after filtration to remove any excitation light and unwanted
fluorescence wavelengths. The excitation illumination is commonly
provided by a high-pressure mercury vapour lamp, which
conveniently provides very high intensity illumination in UV, blue
and green wavelengths. Other high-intensity sources are now also
used, including xenon lamps, lasers (e.g. in confocal microscopes,
which are a highly-derived type of epifluorescence microscope) and
even tungsten-halogen lamps. The separation of excitation and
fluorescence wavelengths is usually accomplished using a dichroic
beam splitter which reflects green, for example, and transmits
orange/red. Further fine-tuning of the excitation wavelengths may
be done using dichroic filters. Many microscope manufacturers
provide filter systems as beam-splitter cube assemblies with all the
filters and dichroic reflectors required for a particular fluorochrome
installed in one pre-aligned package for easy exchange. The
fluoresced light may be further filtered for viewing or photography by
"barrier" filters either of dichroic type, or of coloured glass or
sometimes gelatin (e.g. Kodak Wratten).

Other than that, an epifluorescence microscope can be a normal
compound microscope, but because efficiency of illumination and
collection of light is important, and is limited by the objective lens,
fluorescence microscopes usually employ high quality objectives
with very high numerical aperture. If UV is used, it may be
necessary to select ojectives with very low UV absorption.

Hope this helps
Chris
Date sent: Mon, 5 Mar 2001 17:25:50 -0600
To: Microscopy-at-sparc5.microscopy.com
} From: ileyozerlat-at-yahoo.com ()


The FEI XL30SFEG costs in the region of £250k in UK
The closest Hitachi competitor is the S4700.
The FEI is thermal field emission, the Hitachi is Cold cathode field
emission. There is no Hitachi ESEM, but they do a variable
pressure tungsten filament SEM.

Philips / FEI service in UK is excellent. I have no first hand
knowledge of the Hitachi service operation, but believe it is also
first rate. Hitachi quote much lower prices for their service
contracts than FEI. They tell me that their engineers generally have
little to do because the instruments are so reliable! I don't know
whether this is a true reflection of the cost of ownership, however.

Date sent: Tue, 06 Mar 2001 02:03:30 -0800
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
} From: Gary Gaugler {gary-at-gaugler.com}


Hello,

Our Siemens Elmisjop 102 is presently out of order. We are looking for
several parts of a Siemens Elmiskop 102 which must be replaced.

Parts List C73000 - E3174 - C1 - 1

Part N°335 - Objective current control fine / superfine - cat n° C72315 -
A29 - A4

Part N°333 - Objective current control coarse I level - cat n° C72315 - A31
- B1

Part N°334 - Objective current control medium II level - cat n° C72315 - A31
- B2

Part N°455 - P.C. board V9 "Objective controller" LR - cat n° C72302 - A41 -
A2


Many thanks in advance

Best regards

Philippe Drouillon
Solvay Research and Technology
Electron Microscopy and Image Analysis - Coordinateur Informatique de
Division
Rue de Ransbeek, 310
B-1120 Brussels (Belgium)
phone : (00 32) 2 264 24 47 mailto:philippe.drouillon-at-solvay.com




From daemon Tue Mar 6 09:03:35 2001



From: Neal D. Evans :      evansnd-at-ornl.gov
Date: Tue, 06 Mar 2001 09:57:40 -0500
Subject: Re: EMISPEC and other related systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anita,

We have an Emispec Vision system on our CM200FEG TEM-STEM to acquire
spectrum lines and spectrum images from our Oxford EDS and Gatan GIF. We
tune the GIF using the autofilter functions, and setup the GIF with
ImageFilterControl, but let ESVision read out the GIF multiscan camera
during acquisition. All in all, we are quite pleased with the system. If
you would like to discuss this offline, please contact me directly.

Regards,
Neal



From daemon Tue Mar 6 12:24:30 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 06 Mar 2001 13:22:46 -0500
Subject: Re: 1um section staining for neurons vs. glia (LM/TEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


awgrossm wrote:

} Hi,
}
} I am trying to distinguish between neurons and glia in
} cortical tissue that has been embedded in epoxy resin
} (LX112/NMA/DDSA/DMP-30). Does anyone know how to do
} this on semithin sections? Toluidine-Blue staining
} imparts some subtle differences in the appearance of
} chromatin in the nuclei of neurons vs. glia, but i am
} looking for a staining procedure that provides a more
} obvious color difference (without going all the way to
} immunohistochemistry).

I think you are going to have to rely on morphology alone. Get a copy of
Peters, Palay and Webster, "Fine Structure of the Nervous System". You
should be able to extrapolate from the low mag EMs to LM. You could probably
do an immuno for GFAP (most but not all astrocytes) but that still leaves
oligos and microglia. I don't know of a good immuno for these cells on
plastic sections. Plus, there will be smaller neurons to contend with.
Sorry!

} It is my understanding also that KMnO4 will react with
} NMA (even if i disolve the resin out??), so those
} procedures are out, unless I can find a substitute for
} KMnO4.

And the KMnO4 is for ??


Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Mar 6 13:33:07 2001



From: Holly Aaron :      hollya-at-socrates.berkeley.edu
Date: Tue, 6 Mar 2001 11:28:40 -0800
Subject: job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists –

There is an official open position now at Genentech (the one I alluded to in
an earlier post, but HR is not too speedy over there) . The posting is
below and can also be found on their website: http:// www.genentech.com
{http://www.genentech.com/} .

For immediate consideration please send a c.v. to Peter Schow at
pschow-at-gene.com {mailto:pschow-at-gene.com} .
He is not on the list, so please email him directly.
I have already forwarded previous resumes I received to him, so no need to
resend those.

Thank you,
Holly Aaron


----------------------------------------------------------------------------
----

Position: Research Associate- Cytometry Lab
Requisition #: 01-0003250


Description
Research Associate position available in the Research Cytometry Lab. The lab
support core facility, collaborative and basic research functions. Primary
responsibilities include operation and maintenance of flow cytometers and/or
imaging instrumentation and may cover all aspects of data acquisition,
analysis, interpretation and presentation in the contexts of basic and
applied research. Lab supports Coulter and BDIS flow cytometers, Leica
(confocal) and Nikon (digital fluorescence) microscopes and both Mac and PC
computers. The person will work in a dynamic and interactive environment
requiring a strong teamwork orientation, good communication skills,
flexibility and the ability to interact with a diversity of research
personnel.

Requirements
Position requires a BS/MS and extensive experience with flow and/or image
cytometry. Good working knowledge of cell and molecular biology necessary.
Industrial experience is desirable.



----------------------------------------------------------------------------


Holly Aaron
Head, Berkeley Imaging Center
University of California, Berkeley
Dept. of Molecular and Cell Biology
447 LSA
Berkeley, CA 94720-2751
hollya-at-socrates.berkeley.edu




From daemon Tue Mar 6 15:59:48 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 6 Mar 2001 13:55:14 -0800
Subject: How to measure film thickness?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

How does one measure the thickness of an estimated 20 to 100 nm titanium
dioxide film on glass?

Can it be done via TEM or SEM, or do we need a completely different approach?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Mar 6 16:51:21 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 6 Mar 2001 16:48:17 -0600 (CST)
Subject: Lawsuit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Northwestern University is drafting a complaint against a vendor
of microscopy products, AMT. Has anyone else had experience with
lawsuits against vendors?

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html




From daemon Tue Mar 6 17:22:02 2001



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Tue, 6 Mar 2001 17:19:24 -0600
Subject: Administrivia: Lawsuit - Commentary and Listserver Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

When issues concerning items like this occur please
refrain from any specifics on the Microscopy Listserver.

It is fine to pose a question to ask if anyone has experience
with a legal action against a manufacturer or vendor, but
you should not identify any specific organizations,
vendors, or manufacturers as the target of such action.

This type of posting could be construed as
using the Microscopy Listserver to defame a individual or
organization, in the mind's eye of the community and is completely
against the Listserver rules of operation. The Listserver
is not a forum to "flame" either an individual or a company regardless
of the degree to which you feel that you or your
organization have been wronged.

If you are unsure about a posting, feel free to contact me
off-line first and I will make myself available to comment as to
its appropriateness.

Nestor
Your Friendly Neighborhood SysOp











===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================




From daemon Tue Mar 6 22:19:44 2001



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Tue, 06 Mar 2001 23:20:03 -0500
Subject: Tangential question: laser vision correction for microscopists?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering this, but have read about issues of contrast
perception and/or low light vision. It occurred to me that
microscopists, particularly EM types, depend on their eyes in
low-light situations more than any other profession I can think
of, with the possible exception of cat burglars.

Has anyone out there had LASIK or similar procedure, and
has it been positive or negative for your work? Did you
notice reduced contrast sensitivity?

Thanks for any personal experiences; since it's not a direct
microscopy query, please send replies to me and (if there's
interest) I'll summarize for the list.

Rick Mott (myopic, -7 or so, with some astigmatism)




From daemon Wed Mar 7 00:25:10 2001



From: erich-at-ento.csiro.au (Eric Hines)
Date: Wed, 7 Mar 2001 17:22:16 +1100
Subject: SEM cryo-stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

We have a ten year old cryo-stage and transfer device for sale.

It is a BioRad E7400 comprising:
Pump valve controller
Sputtering module
Evaporation module
Edwards E2M5 rotary pump x2
Valve block
Transfer/coating unit with dewar
Stage cooling unit with dewar
Specimen insertion rod
ie all you need to go cryo-SEMing.

The rod and flanges suit a JEOL 6400 SEM. The unit was functioning and in
good condition prior to replacement. Any offers?

Eric Hines
Microscopy Centre
CSIRO Entomology
Canberra.




From daemon Wed Mar 7 04:12:23 2001



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Wed, 7 Mar 2001 11:04:24 +0100 (MET)
Subject: looking for a stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I send you the following message written by a serious student. I hope that
someone can offer a possibility to her.
Thanh you very much. All the best,
Elena Belluso


My name is Elisa Fornero and I doing my Degree thesis, at Turin University -
Italy- (Dept. of Mineralogical and Petrologic Sciences), on "INORGANIC
FIBERS IN LUNG TISSUES: MINERALOGICAL CHARACTERIZATION, COMPARISION WITH
NATURAL ASBESTOS AND CALCULATION OF DIFFERENT FIBROUS MINERALOGICAL SPECIES"
(the samples of tissues derived from people not professionally exsposed in
order to value the environmental pollution of fibrous minerals).
During my thesis I extensively worked with SEM and EDS and I learned to
prepare the samples from lungs tissues filtrated.
I would like to have some international experience taking part, about for
two months between April and June 2001, to a stage in a foreigner
University. I would like found a laboratory where develop my knowledge in
this field.
If there is anyone interested to host me, I can send my curriculum vitae,
Thank you
Elisa Fornero



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011 670 7135 - fax: (39) 011 670 7128
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------


"I've... seen things you people wouldn't believe.
Attack ships on fire off the shoulder of Orion.
I watched C-beams... glitter in the dark near the Tanhauser Gate.
All those... moments will be lost... in time...,
like... tears... in... rain."

Blade Runner



From daemon Wed Mar 7 04:12:23 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 7 Mar 2001 11:08:39 +0100
Subject: Re : How to measure film thickness?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



It should be possible to do this much faster and without any sample
preparation with X ray reflectometry.

See web site :

http://www-cxro.lbl.gov/optical_constants/

The is there the possibility to simulate some mesures.

Optical methodes could also be able to do this, by interferences in the
TiO2 film.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Wed Mar 7 04:12:23 2001



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Wed, 7 Mar 2001 11:05:15 +0100 (MET)
Subject: looking for a stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I send you the following message written by a serious student. I hope that
someone can offer a possibility to her.
Thanh you very much. All the best,
Elena Belluso


My name is Elisa Fornero and I doing my Degree thesis, at Turin University -
Italy- (Dept. of Mineralogical and Petrologic Sciences), on "INORGANIC
FIBERS IN LUNG TISSUES: MINERALOGICAL CHARACTERIZATION, COMPARISION WITH
NATURAL ASBESTOS AND CALCULATION OF DIFFERENT FIBROUS MINERALOGICAL SPECIES"
(the samples of tissues derived from people not professionally exsposed in
order to value the environmental pollution of fibrous minerals).
During my thesis I extensively worked with SEM and EDS and I learned to
prepare the samples from lungs tissues filtrated.
I would like to have some international experience taking part, about for
two months between April and June 2001, to a stage in a foreigner
University. I would like found a laboratory where develop my knowledge in
this field.
If there is anyone interested to host me, I can send my curriculum vitae,
Thank you
Elisa Fornero



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011 670 7135 - fax: (39) 011 670 7128
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------


"I've... seen things you people wouldn't believe.
Attack ships on fire off the shoulder of Orion.
I watched C-beams... glitter in the dark near the Tanhauser Gate.
All those... moments will be lost... in time...,
like... tears... in... rain."

Blade Runner



From daemon Wed Mar 7 04:41:50 2001



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 7 Mar 2001 11:35:34 +0100
Subject: analysis of EDS X-ray spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear fellows microscopists,

we are interested in purchasing a good software for desktop-
analysis (on a PC) of X-ray EDS spectra, previously acquired
on an analytical TEM and transferred to other computer.

In the book of Williams and Carter is mentioned only one such
program, i.e. the DTSA, from NIST. Does anyone of you know
a web address where I could get more informations about this
software ? I would be delighted if there will be any possibility to
check it before ordering, but where or how?
Besides, does anyone know about a better program of this kind ?
We would much better appreciate a free one, but we are ready to
purchase even a commercial one.

Thank you for your attention.

Corneliu Sarbu, PhD
Metallyrgy and Applied Materials Science Dept.
Catholic University of Leuven
Belgium



From daemon Wed Mar 7 05:04:27 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 07 Mar 2001 08:31:14 -0500
Subject: I've got the screws loose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----------
} From: "CAROL.A.WALDER" {eencaw-at-elec-eng.leeds.ac.uk}
To: mtlrmdb-at-leeds.ac.uk


Hi Listers,

I have a LKB Bromma 11800 Pyramitome whose belt has come loos and it needs to be replaced. I can't find the instruction manual or the slip of paper that my predecessor wrote the service person's name on.

Does anybody out there have a source for the instruction manual? I called Leica but they told me this machine was absolete when they acquired the LKB line.

I'll fix this thing myself if anyone can send me a copy of the manual (I'll glaly pay for the cost of copying and shipping).

If anyone knows of a repair person who still works on these ancient beasties, please let me know. It would be good to have someone who could give them tune-ups every now & then.

My screws are loose and I've got the hammer, blow torch sosme bubble gum and bailing wire....now all I need is the manual.


Pretty please??

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed Mar 7 07:46:47 2001



From: John Henry J. Scott :      johnhenry.scott-at-nist.gov
Date: Wed, 07 Mar 2001 09:02:26 -0500
Subject: Re: analysis of EDS X-ray spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,

An excellent write-up about the process is located at
http://www.manufacturing.net/magazine/dn/archives/current/feature1.html

Cheers
Peter Tarquinio
peter-at-evex.com


Evex Analytical
Microanalysis and Digital Imaging
857 State Road
Princeton, NJ 08540
609-252-9192 T
609-252-9091 F
www.evex.com
info-at-evex.com
----- Original Message -----
} From: "Rick Mott" {rickmott-at-alumni.princeton.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, March 06, 2001 11:20 PM



} In the book of Williams and Carter is mentioned only one such
} program, i.e. the DTSA, from NIST. Does anyone of you know
} a web address where I could get more informations about this
} software ? I would be delighted if there will be any possibility to
} check it before ordering, but where or how?
} Besides, does anyone know about a better program of this kind ?
} We would much better appreciate a free one, but we are ready to
} purchase even a commercial one.
}
} Thank you for your attention.
}
} Corneliu Sarbu, PhD
} Metallyrgy and Applied Materials Science Dept.
} Catholic University of Leuven
} Belgium


Corneliu,

DTSA is no longer sold as a Standard Reference Data product. NIST has
abrogated its patent on DTSA so both the executable and source code are
available from the following URL free of charge:

http://www.cstl.nist.gov/div837/837.02/MicroscopySoftware.html

If you find it does not meet your needs, please drop me an email. Our group
welcomes suggestions for new features or improvements.

-- John Henry

John Henry J. Scott Bldg 222/Rm A113
NIST Microanalysis Research Group 100 Bureau Drive Stop 8371
(301) 975-4981; (301) 417-1321 FAX Gaithersburg, MD 20899-8371



From daemon Wed Mar 7 08:21:16 2001



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Wed, 7 Mar 2001 08:20:24 -0600
Subject: looking for a stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I send you the following message written by a serious student. I hope that
someone can offer a possibility to her.
Thanh you very much. All the best,
Elena Belluso


My name is Elisa Fornero and I doing my Degree thesis, at Turin University -
Italy- (Dept. of Mineralogical and Petrologic Sciences), on "INORGANIC
FIBERS IN LUNG TISSUES: MINERALOGICAL CHARACTERIZATION, COMPARISION WITH
NATURAL ASBESTOS AND CALCULATION OF DIFFERENT FIBROUS MINERALOGICAL SPECIES"
(the samples of tissues derived from people not professionally exsposed in
order to value the environmental pollution of fibrous minerals).
During my thesis I extensively worked with SEM and EDS and I learned to
prepare the samples from lungs tissues filtrated.
I would like to have some international experience taking part, about for
two months between April and June 2001, to a stage in a foreigner
University. I would like found a laboratory where develop my knowledge in
this field.
If there is anyone interested to host me, I can send my curriculum vitae,
Thank you
Elisa Fornero



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011 670 7135 - fax: (39) 011 670 7128
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------


"I've... seen things you people wouldn't believe.
Attack ships on fire off the shoulder of Orion.
I watched C-beams... glitter in the dark near the Tanhauser Gate.
All those... moments will be lost... in time...,
like... tears... in... rain."

Blade Runner




From daemon Wed Mar 7 09:47:39 2001



From: Staman, John :      jstaman-at-lsil.com
Date: Wed, 7 Mar 2001 08:37:58 -0700
Subject: How to measure film thickness?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan,

My vote would be a TEM. Of course, if the film is patterned in any way,
an AFM measurement of the step would be very quick and much cheaper.

Regards,

John Staman
LSI Logic. Colorado
Analytical Services
719-573-3282

-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Tuesday, March 06, 2001 2:55 PM
To: Microscopy-at-sparc5.microscopy.com


Hi:

How does one measure the thickness of an estimated 20 to 100 nm titanium
dioxide film on glass?

Can it be done via TEM or SEM, or do we need a completely different
approach?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Mar 7 09:47:39 2001



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 07 Mar 2001 09:44:48 -0600
Subject: DTSA info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Corneliu Sarbu,

You can find information about DTSA at the NIST web site

http://www.cstl.nist.gov/div837/837.02/dtsa.html

There is only a Macintosh version of this software, so it may not be
of use for you if you are looking for software for a PC. NIST has
officially withdrawn DTSA as a Standard Reference Data product and has
abrogated its patent. NIST is now releasing the software free from
charge, however no longer officially supports the software except for
internal development as an internal research tool for NIST. The NIST
web site I directed you to has links to download the software
application and the Pascal source code.

Tyrone Daulton

} Dear fellows microscopists,

} ...
} we are interested in purchasing a good software for desktop-
} analysis (on a PC) of X-ray EDS spectra, previously acquired
} on an analytical TEM and transferred to other computer.

} In the book of Williams and Carter is mentioned only one such
} program, i.e. the DTSA, from NIST. Does anyone of you know
} a web address where I could get more informations about this
} software ? ...

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Wed Mar 7 10:04:25 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 7 Mar 2001 10:57:10 -0500 (EST)
Subject: Re: I've got the screws loose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

Jon Pett at TekNet in NJ has worked on a lot of different kinds of
microtomes. You might call him for service/information. (908) 905-5530.

Sara



Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Mar 7 10:49:02 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 7 Mar 2001 11:38:30 -0500 (EST)
Subject: Histotechnologist Position Open (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am reposting this for a colleague. If interested, please reply directly to

Ms. Linda McGuire
lmcguire-at-downstate.edu


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Experienced Histotechnologist to work in Neuropathology Research
Laboratory of Department of Pathology

The person we seek will be responsible for maintaining a neuropathology
research laboratory in the Department of Pathology at SUNY Downstate
Medical Center. Knowledge of immunohistochemistry, special stains, and
histopathology is required. Previous experience in handling central
nervous system tissue preferred. The individual will work with only
minimum supervision.

Position Requirements

Minimum of five years experience working in a laboratory with hands-on
experience in tissue processing, histochemistry, immunohistochemistry,
and operation of a light microscope. Experience with technical aspects of
neuropathology and performing special stains including silver
(Bielschowsky) and myelin stains.

Two years experience working on immunohistochemistry of CNS tissue
specimens with knowledge of immunoreagents and experimental protocols.

BachelorUs degree in science desirable.

Laboratory skills including communications, ability to comply with safety
and laboratory regulations, maintenance of laboratory equipment and
resources, operation of computers and office equipment.

Advanced computer skills (word processing and database management) essential.

Desirable Experience

Confocal microscopy desirable.

Salary commensurate with experience


Responsibilities

Purchasing supplies and equipment, budget reports, laboratory maintenance
and brain banking.

Will operate all microscopic, photographic and computer equipment, and
keep accurate records of all laboratory experiments and procedures. Light
and fluorescent microscopy; tissue processing for paraffin embedding,
sectioning and slide stainer for immunohistochemical procedures; computer
imaging (PhotoShop); general photography; and library and web searches

Familiarity with computer software for keeping records, report
preparation and table/figure construction using Microsoft Office software
(Word, Excel, PowerPoint) as well as Endnote and Adobe PhotoShop.

Send resume to:

Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-------------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265







From daemon Wed Mar 7 10:49:07 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 7 Mar 2001 11:43:51 -0500 (EST)
Subject: Confocal Technical Position Open (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am reposting this for a colleague. If interested, please reply
directly to

Ms. Linda McGuire
lmcguire-at-downstate.edu
------------------------------------------------------------------------

Experienced Confocal Microscopy/Electron Microscopy Technologist to work
in Research Laboratory of Department of Pathology


The person we seek will be responsible for organization of a new facility
that includes a confocal microscope and an electron microscope. The
position includes overall management of the microscopy facility, user
training, and user supervision. Requirements for the position include
experience with light and transmission electron microscopy. This
individual will oversee all aspects of specimen accession and processing,
operation of the microscopes, photography, record keeping, and
supervision of a technician. Knowledge of EM, biology, and pathology, as
well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Experience with confocal and digital imaging techniques, microinjection,
visualization of living cells containing fluorescent probes,
photobleaching, and fluorescence in situ hybridization.

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

Excellent interpersonal and organizational skills are essential.

BachelorUs degree in science desirable.

Desirable Experience

Expertise in training in the operation of confocal microscope systems is
a distinct advantage.

Familiarity with light microscopy methods, immunofluorescent staining,
use of fluorescent probes for physiologic measurements and the general
principles of cell biological research are critical. Significant facility
with computers is desired.

Responsibilities

Serve as the technical manager of the facility and be responsible for the
operation and maintenance of the confocal and EM microscope facility.

Perform routine transmission EM, including tissue processing,
ultramicrotomy, and examination; do preventative maintenance on the
equipment; maintain the lab, order supplies, schedule instruments, and
oversee billing.

Image analysis at the light, confocal and electron microscopic levels and
preparation of micrographs for publication.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265







From daemon Wed Mar 7 10:53:43 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 7 Mar 2001 11:44:31 -0500 (EST)
Subject: EM Tech Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am reposting this for a colleague. If interested, please reply
directly to

Ms. Linda McGuire
lmcguire-at-downstate.edu

-----------------------------------------------------------------------
Experienced Electron Microscopy Technologist to work in Research
Laboratory of Department of Pathology


The person we seek will be responsible for the overall operation of the
EM laboratory in the Department of Pathology at SUNY Downstate Medical
Center. This individual will oversee all aspects of specimen accession
and processing, operation of the microscope, photography, record keeping,
and supervision of a technician. Knowledge of EM, biology, and pathology,
as well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

BachelorUs degree in science desirable.

Laboratory management skills including effective written/verbal
communication skills to interact with a diverse group, ability to comply
with safety and laboratory regulations, maintenance of laboratory
equipment and resources, and operation of computers and office equipment.

Desirable Experience

Previous experience in confocal microscopy highly desirable.

Previous experience in performing immunocytochemical staining and
advanced computer skills usage (e.g. image analysis) is also desirable.

Salary commensurate with experience

Responsibilities

Maintain electron microscope in operating condition. Process clinical and
research tissues for "thick" and "thin" sectioning. Darkroom management
of photographic printing.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by:
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265







From daemon Wed Mar 7 10:55:22 2001



From: allen-at-aaem.amc.anl.gov (Charles W. Allen)
Date: Wed, 7 Mar 2001 10:53:44 -0600
Subject: MidWest Microscopy and Microanalysis Society and ASM Joint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times {/param} {bigger} SCANNING ELECTRON MICROSCOPY
(SEM) AND ENERGY DISPERSIVE

X-RAY SPECTROSCOPY (EDS)

WITH APPLICATIONS AT VARIOUS PRESSURES AND ATMOSPHERES


AN EDUCATIONAL SEMINAR-FRIDAY, APRIL 27, 2001

MOTOROLA GALVIN CENTER-SCHAUMBERG, IL

8:30 AM - 5:00 PM


{underline} Instructors {/underline} : Vern Robertson, JEOL-USA, and
Nestor Zaluzec, Argonne National Laboratory.

{underline} Time {/underline} : Check-in at Galvin Center 8:30-9:00 am.
Seminar 9:00-12:30 and 1:30-5:00.

{underline} Preregistration Required {/underline} : Deadline April 20,
2001; no walk-ins.

{underline} Cost {/underline} : Members of ASM-International and of the
Midwest Microscopy and Microanalysis Society-$30; Non-members-$50;
Students: $10. Registration includes lunch and refreshments at breaks.
Check or money order to accompany preregistration form (below).

{underline} Exhibits {/underline} : Representatives from the following
industrial co-sponsors will be present to discuss your applications and
their products: EDAX, FEI, Hitachi, JEOL-USA, LEO, Noran, Oxford, and
PGT.

{underline} Directions to Seminar Site {/underline} : Please see the other
side.

{underline} Preregistration {/underline} : Please complete the following
form. Mail it with your check or money order made out to "Chicago
Regional Chapter of ASM-I" to Ms. Sheila Jungman, MSD 212, Argonne
National Laboratory, Argonne, IL 60439 {bold} . {/bold} Deadline for
receipt of the form is April 20, 2001. If you require a receipt for the
preregistration cost, please so indicate below for pick-up at the
Seminar Check-In desk. Questions? Phone 630-252-4157 or e-mail
allen-at-aaem.amc.anl.gov.



{bold} SEM/EDS Educational Seminar-Friday, April 27, 2001

{/bold} Name (please print
clearly)________________________________________________________

Affiliation_______________________________________Phone_________________________

Address_______________________________________________________________________

________________________________________________________________________

ASM-I Member ($30)_______; MMMS Member ($30)________; Non-Member
($50)________; Student ($10)______If student,
where_______________________________________________

Please enclose check or money order made payable to "Chicago Regional
Chapter ASM-I". Do you need a receipt ?______. Mail form and payment
to

Ms. Sheila Jungman, MSD 212, Argonne National Laboratory, Argonne, IL
60439



{/bigger} {/fontfamily} ========================================

Charles W. Allen

Electron Microscopy Center-HVEM-Tandem Facility

MSD 212/E211

9700 South Cass Avenue

Argonne National Laboratory

Argonne. IL 60439 USA


Email:allen-at-aaem.amc.anl.gov

Tel: 630-252-4157 Fax:630-252-4798 or -4298


Home: Niles, MI. allen.42-at-nd.edu


========================================




From daemon Wed Mar 7 12:26:15 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 7 Mar 2001 12:21:35 -0600 (CST)
Subject: Brittle samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are having problems with preparing brittle TEM sample of (single
crystal) sapphire and magnesium orthovanadate via dimpling & PIPS. Any
suggestions beyond a kid-gloves approach?

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Mar 7 12:45:31 2001



From: allen-at-aaem.amc.anl.gov (Charles W. Allen)
Date: Wed, 7 Mar 2001 12:43:26 -0600
Subject: MMS and ASM Joint Meeting : Driving Directions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times {/param} {bigger} Direction to the MMMS and ASM
meeting in Schaumburg Il on April 27, 2001




LOCATION: Motorola Galvin Center Auditorium. Motorola Center

occupies the Southwest corner at the intersection of Algonquin and

Meacham Roads, Schaumberg, IL


{underline} All attendees {/underline} must enter at the Algonquin Road
"A" Entrance.

Do not stop at the Visitors Center. Proceed to the guard station

in the center of the roadway and inform the guard that you are

going to the Galvin Center Auditorium. Go straight until making

a right turn onto Center Drive. Then make a left turn into the Galvin

Center parking lot. Enter through the {underline} Museum
Entrance {/underline} near the

east side of the building.


ADDITIONAL DIRECTIONS: From the Northwest Tollway (I-90).

Exit I-90 at Roselle Road and turn right (north). Follow Roselle Road


to Algonquin Road (Rte 62) and turn right (east). Proceed east past

several stoplights to the Motorola Center campus. Turn right at
Entrance

A (West Drive). (If you pass Meachem Road, you have gone too far on

Algonquin Road.) OR From Rte 53, take Algonquin Road (Rte 62)

west. After passing Meachem Road, turn left at the second entrance

(Entrance A-West Drive) to Motorola Center.


ABOUT THE SEMINAR AND ITS INSTRUCTORS:

Both Vern Robertson and Nestor Zaluzec are well known for their clear

and informative presentations in the areas of SEM and EDS. Both topics


will be developed from the basics with an abundance of helpful visual

aides. Special emphasis will be placed by both instructors on
instrumentation,

techniques and problems of interpretation and quantitation, including

when the specimen environment is not vacuum. During the morning

and afternoon breaks and during a portion of the lunch hour,
representatives

of eight co-sponsoring manufacturers will be available for interaction
with

other attendees.


This seminar has been organized by members of the

Chicago Regional Chapter of ASM-International in cooperation

with the Midwest Microscopy and Microanalysis Society, the

Materials Science Division of Argonne National Laboratory, JEOL-USA and
Motorola.



{/bigger} {/fontfamily}



From daemon Wed Mar 7 13:16:36 2001



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Wed, 07 Mar 2001 14:16:09 -0500
Subject: Re: forensic microscopy course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary-Jacque,
Several years ago at a used book library sale, I found a copy of
Forensic Geology-Earth Sciences and Criminal Investigation by Raymond C.
Murray and John C.F. Tedrow. It dates from 1975 and looks interesting. I
hope to read more of it.
Another title which came from a special session at the MAS-MSA
meeting in 1985 is titled Electron Microscopy in Forensic, occupational and
environmental health sciences by Samarendra Basu and James R. Millette.
I still lament that the Microbeam Analysis Society dropped
Sherlock Holmes' hat and pipe from their logo.




John Hunt
CCMR Microscopy Facility
255-0108



From daemon Wed Mar 7 13:22:01 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 7 Mar 2001 14:03:48 -0500
Subject: RE: Brittle samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lawry,
I don't know about magnesium orthovanadate, but sapphire will work using the small angle cleavage technique. I have done cross sections of GaN on sapphire when I taught some students at Univ. of Illinois how to do it. John McCaffrey has done YBCO on cerium oxide on sapphire. If you are only interested in the bulk, that is even easier. If the magnesium orthovanadate is as brittle as you say, then SACT will probably work on that also. If you want, I can send you an image of the GaN/sapphire.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: L. D. Marks [mailto:ldm-at-risc4.numis.nwu.edu]
} Sent: Wednesday, March 07, 2001 1:22 PM
} To: Microscopy List
} Subject: Brittle samples
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} We are having problems with preparing brittle TEM sample of (single
} crystal) sapphire and magnesium orthovanadate via dimpling & PIPS. Any
} suggestions beyond a kid-gloves approach?
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} -------------------------------------------------------
}
} Workshop May 17-19 2001 "New approaches to the Phase Problem"
} http://xraysweb.lbl.gov/esg/phasing/index.html
}
}


From daemon Wed Mar 7 13:42:20 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 7 Mar 2001 13:38:12 -0600 (CST)
Subject: Brittle samples - NOT CROSS-SECTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A quick clarification before I get deluged with responses about
how to do cross-sections; we are not trying to do this. All (?)
we want is a "conventional" 3mm disc sample of sapphire or
magnesium orthovanadate. No glue, no mounting on a Cu ring,
no nothing.

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Mar 7 13:45:53 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 07 Mar 2001 12:32:58 -0800
Subject: Re: Brittle samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Dr. Marks:

I think a good solution may be Tripod Polishing. We have an application note on preparing samples
of sapphire with a GaN film which may be useful. Let me know if you would like a copy of the note
and I can email it to you.

Best regards-

David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


} RE: Brittle samples
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are having problems with preparing brittle TEM sample of (single
} crystal) sapphire and magnesium orthovanadate via dimpling & PIPS. Any
} suggestions beyond a kid-gloves approach?
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} -------------------------------------------------------

--



From daemon Wed Mar 7 13:53:29 2001



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 07 Mar 2001 15:53:04 -0400
Subject: homemade transmitted electron detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

Has anybody out there had any experiences with making your
own transmitted electron detector for SEM? I'm interested in
sharing ideas, references, etc. I've made one for myself and
am fairly happy with its performance, but am wondering if
there is more that I can do.

Thanks in advance,

Jim



--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Wed Mar 7 13:58:33 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 7 Mar 2001 09:55:21 -1000 (HST)
Subject: TEM - immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two questions for you immunolabeling experts.

First, a client has some precious and irreplaceable histological sections
of human hippocampus that have been labeled with ABC - DAB. Will it be
possible for me to de-paraffinize the sections, expose them to osmium
vapor and re-embed them in resin on the slide and pop them off and
resection them and see the DAB precipitate? Anyone have a protocol that
has worked?

Second, these researchers plan to use Vibratome sections of mouse brain
and immunolabel them for light microscopy using an ABC kit. I have done
on-ultrathin-section colloidal gold immunolabeling for TEM. However, for
this new project we would like to try pre-embedding labeling of 50-60
micrometer Vibratome sections, then embed and resection them for
TEM. Knowing what works for light microscopy, we'd like to use
streptavidin/colloidal gold or whatever for TEM visualization. My question
is, of course, does anyone have a favorite protocol they would be willing
to share? How do I keep the sections from curling? If we stick them on a
glass slide to embed in resin and (hopefully) pop off later for
resectioning, when and how do I get the to adhere?

Mahalo!

Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Mar 7 14:54:12 2001



From: Anita Garg :      Anita.Garg-at-grc.nasa.gov
Date: Wed, 07 Mar 2001 15:48:39 -0500
Subject: Cerius2 from MSI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
Has anybody used Cerius2 software from Molecular Simulations Inc. for new
alloy development? Any comments would be appreciated.



From daemon Wed Mar 7 15:20:48 2001



From: John Henry J. Scott :      johnhenry.scott-at-nist.gov
Date: Wed, 07 Mar 2001 16:17:15 -0500
Subject: new URL for DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The URL I gave this morning for DTSA was correct, but not memorable. A few
hours ago an alias was created to make it easier to navigate to the NIST
Microanalysis Software web page. DTSA can now be accessed from:

http://www.nist.gov/dtsa

This page also contains links to Lispix, Dave Bright's excellent image
processing application (now available for Win9x/WinNT/Win2k) and the NIST
Monte Carlo codes. Lispix can be accessed directly at

http://www.nist.gov/lispix


-- John Henry

John Henry J. Scott Bldg 222/Rm A113
NIST Microanalysis Research Group 100 Bureau Drive Stop 8371
(301) 975-4981; (301) 417-1321 FAX Gaithersburg, MD 20899-8371



From daemon Wed Mar 7 15:30:27 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Wed, 07 Mar 2001 13:25:58 -0800
Subject: RE: Brittle samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try epoxying a thin metal washer on one side of the sample, and dimple from the
washer side.


Larry Thomas
Pacific Northwest National Laboratory
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto:Larry.Thomas-at-pnl.gov


----------
From: L. D. Marks
Sent: Wednesday, March 7, 2001 10:21 AM
To: Microscopy List
Subject: Brittle samples

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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We are having problems with preparing brittle TEM sample of (single
crystal) sapphire and magnesium orthovanadate via dimpling & PIPS. Any
suggestions beyond a kid-gloves approach?

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html





From daemon Wed Mar 7 17:49:20 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Wed, 07 Mar 2001 15:44:23 -0800
Subject: RE: Brittle samples - NOT CROSS-SECTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Laurie,

The purpose of gluing a metal ring to brittle samples before dimpling is
to support the sample. The sample is then waxed to the dimple grinding support
stub and dimpled through the washer. We use this method routinely with brittle
samples and not just cross sections. It's a good idea to carefullly clean the
sample and washer so the epoxy bonds well. One- or two-mil Mo hole or slot
washers usually work well for us, but we sometimes use other washer materials to
avoid confusing Mo in the sample with Mo deposition artifacts.

Larry

----------
From: L. D. Marks
Sent: Wednesday, March 7, 2001 11:38 AM
To: Microscopy List
Subject: Brittle samples - NOT CROSS-SECTION

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


A quick clarification before I get deluged with responses about
how to do cross-sections; we are not trying to do this. All (?)
we want is a "conventional" 3mm disc sample of sapphire or
magnesium orthovanadate. No glue, no mounting on a Cu ring,
no nothing.

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html





From daemon Wed Mar 7 18:11:31 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Wed, 7 Mar 2001 18:11:16 -0600
Subject: RE: countig grain size in archaeological iron samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


James Clatk:

the metallurgy or materials science group in your university should
be able to tell you all about grain size measurements.

Best regards

Sam Purdy
Technical Center
National Steel Corp.
Trenton Mi USA
spurdy-at-nationalsteel.com

} ----------
} From: James Clarke
} Sent: Wednesday, March 7, 2001 11:45 AM
} To: NIH-IMAGE-at-LIST.NIH.GOV
} Subject: countig grain size in archaeological iron samples
}
} IMAGERS!
} Hope some one can point me in the right direction. I need to be
} able to measure the grain size of metal samples which are of a
} heterogenous nature. I am using a PC and Scion image seems to
} have some of the things I need but some advice would be nice.
}
} ---------------------------------
} James Clarke
} J.Clarke1-at-bradford.ac.uk
}




From daemon Wed Mar 7 18:13:28 2001



From: Emma Lou Cardell :      cardelel-at-email.uc.edu
Date: Wed, 7 Mar 2001 18:13:41 -0600
Subject: MT2B Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microtomists:
I am seeking information regarding 2 Sorvall MT2B ultramicrotomes that
still are in use and in good working condition for both semi-thin sections
and ultrathin sections. However, the faster speed of the return stroke of
the cutting cycle no longer engages, even though the duration and position
control belts are in tact for the slow-speed (cutting phase) of the cycle.
Is this a relatively simple repair, and is there a company in the
Cincinnati, Ohio, area that provides such repair service? [The serial
numbers of these microtomes are 7701673 and 7800584. Do the first 2 digits
reflect the year of manufacture (1977 and 1978 respectively)?] What would
be a fair price for microtomes of this ventage?
Thanks in advance for your assistance.
EL Cardell




From daemon Wed Mar 7 18:20:39 2001



From: De McKeown :      de-at-payload.com
Date: Wed, 7 Mar 2001 18:20:41 -0600
Subject: Question: Resolution and TV Lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} Email: de-at-payload.com
} Name: De McKeown
}
} Organization: Payload Systems
}
} Education: Graduate College
}
} Location: Cambridge, MA, USA
}
} Question: I need to determine the number of TV lines my microscope is
} giving me. I have a test target but it only goes to 266 l/mm and I have
} better resolution than that. I can't find a 'better' target and am stuck
} trying to get an answer. How can I do this or where can I go for a new
} target?
}
} I also have to find a contrast, but haven't got enough information yet to
} ask a good question.
}
} Thank you!!
}




From daemon Wed Mar 7 18:36:07 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 7 Mar 2001 18:36:04 -0600
Subject: Administrivia: Subject Lines & Viruses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues.....

There is a rash of virus mail out on the net again, a number
of which are using "suggestive names" in the subject line.
As a precaution some people trash messages which appear
out of context.

While Humorous Titles are good for the soul (especially mine) and bring
a smile especially when one gets the implied joke, let me remind you
to at least add in the Subject Line with some standard notation so that
individuals can more easily judge source of the mail. After all, we know
that my Email filtering system is not perfect and suspect mail leaks
through every so often.

Some Keywords include: TEM, SEM, EDS, .Confocal, LM, SamPrep, etc...
check the FAQ site if you need idea's, but you can easily see what
I mean.

For example: the posting on "I've got the screws loose" could have be
preceeded or prepended by an appropriate Keyword like Microtome,
even if the word Microtome was in () or abbreviated as uTome.

Cheers....

Nestor
Your Friendly Neighborhood SysOp





From daemon Wed Mar 7 18:38:52 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 7 Mar 2001 18:36:36 -0600 (CST)
Subject: RE: Brittle samples - NOT CROSS-SECTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unfortunately getting Mo (or anything else) on the sample destroys it
as far as I am concerned. So, we have to rule out anything like this
unless we can safely remove the metal with an acid (and not dissolve
the oxide in the process).

On Wed, 7 Mar 2001, Thomas, Larry (PNNL) wrote:

} Laurie,
}
} The purpose of gluing a metal ring to brittle samples before dimpling is
} to support the sample. The sample is then waxed to the dimple grinding support
} stub and dimpled through the washer. We use this method routinely with brittle
} samples and not just cross sections. It's a good idea to carefullly clean the
} sample and washer so the epoxy bonds well. One- or two-mil Mo hole or slot
} washers usually work well for us, but we sometimes use other washer materials to
} avoid confusing Mo in the sample with Mo deposition artifacts.
}
} Larry
}
} ----------
} From: L. D. Marks
} Sent: Wednesday, March 7, 2001 11:38 AM
} To: Microscopy List
} Subject: Brittle samples - NOT CROSS-SECTION
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A quick clarification before I get deluged with responses about
} how to do cross-sections; we are not trying to do this. All (?)
} we want is a "conventional" 3mm disc sample of sapphire or
} magnesium orthovanadate. No glue, no mounting on a Cu ring,
} no nothing.
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} -------------------------------------------------------
}
} Workshop May 17-19 2001 "New approaches to the Phase Problem"
} http://xraysweb.lbl.gov/esg/phasing/index.html
}
}
}
}

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Mar 7 18:53:29 2001



From: Michelle Peiffer :      mlk101-at-psu.edu
Date: Wed, 07 Mar 2001 19:47:14 -0500
Subject: IEM high background

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

A researcher has asked me to localize gluconase (a protein) in plant roots.
They provided me with antibody (sera) and preimmune, which they have used
in westerns. The problem is the preimmune is labelling the cell wall; the
labeling is quite impressive and very specific. Now they tell me (alas I
didn't ask before) that yes they have background on the westerns, but the
preimmune never labels the 32 Kd protein they are interested in. What is
the best approach to localize this 32 Kd protein? Should we cross absorb
everything else out, or would it be better to affinity purify for the
desired antibody. And why does this rabbit have such good antibodies to
cell wall?

Thank you,
Michelle


Michelle Peiffer
*************************************************************
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email: mlk101-at-psu.edu
**************************************************************



From daemon Wed Mar 7 21:55:46 2001



From: Gordon Nord :      gnord-at-mindspring.com
Date: Wed, 07 Mar 2001 22:52:18 -0500
Subject: Re: Brittle samples - NOT CROSS-SECTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The key to thinning brittle hard samples is to start with a doubly polished thin
section 30 micrometers thick.
Cut out 3 mm diameter discs with an ultrasonic probe.
Glue these to a glass slide with crystalbond, a thermal plastic cement that dissolves
in acetone.
Mechanically thin and polish to less than 10 micrometers or thinner.
Ion thin until satisfied.

Start thinning a thin sample. Don't break it. One good sample is all you need.

I have made many ion thinned samples of brittle materials, mainly shocked minerals,
for over thirty years.
Also this method is not for those in a hurry.

Gordon Nord
USGS Emeritus Scientist

"L. D. Marks" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Unfortunately getting Mo (or anything else) on the sample destroys it
} as far as I am concerned. So, we have to rule out anything like this
} unless we can safely remove the metal with an acid (and not dissolve
} the oxide in the process).
}
} On Wed, 7 Mar 2001, Thomas, Larry (PNNL) wrote:
}
} } Laurie,
} }
} } The purpose of gluing a metal ring to brittle samples before dimpling is
} } to support the sample. The sample is then waxed to the dimple grinding support
} } stub and dimpled through the washer. We use this method routinely with brittle
} } samples and not just cross sections. It's a good idea to carefullly clean the
} } sample and washer so the epoxy bonds well. One- or two-mil Mo hole or slot
} } washers usually work well for us, but we sometimes use other washer materials to
} } avoid confusing Mo in the sample with Mo deposition artifacts.
} }
} } Larry
} }
} } ----------
} } From: L. D. Marks
} } Sent: Wednesday, March 7, 2001 11:38 AM
} } To: Microscopy List
} } Subject: Brittle samples - NOT CROSS-SECTION
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } A quick clarification before I get deluged with responses about
} } how to do cross-sections; we are not trying to do this. All (?)
} } we want is a "conventional" 3mm disc sample of sapphire or
} } magnesium orthovanadate. No glue, no mounting on a Cu ring,
} } no nothing.
} }
} } -------------------------------------------------------
} } Laurence Marks
} } Department of Materials Science and Engineering
} } Northwestern University
} } Tel: (847) 491-3996 Fax: (847) 491-7820
} } mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} } -------------------------------------------------------
} }
} } Workshop May 17-19 2001 "New approaches to the Phase Problem"
} } http://xraysweb.lbl.gov/esg/phasing/index.html
} }
} }
} }
} }
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
} -------------------------------------------------------
}
} Workshop May 17-19 2001 "New approaches to the Phase Problem"
} http://xraysweb.lbl.gov/esg/phasing/index.html

--
Gordon Nord
Small Business Network Design and Construction
Macintosh and Windows - Solutions and Conflicts

Nord Consultants
20594 Cornstalk Terrace
Ashburn VA 20147

Voice 703-723-2798 (Home Office)
Cell 703-403-2776 (Mobile Office)
Email gnord-at-mindspring.com




From daemon Wed Mar 7 22:59:12 2001



From: linda_knoll-at-msn.com
Date: Wed, 7 Mar 2001 22:57:12 -0600
Subject: Ask-A-Microscopist: cells of humans and apes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(linda_knoll-at-msn.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, March
7, 2001 at 18:43:01
---------------------------------------------------------------------------

Email: linda_knoll-at-msn.com
Name: Linda Knoll

Organization: St.Joseph'sAcademy

Education: Graduate College

Location: St. Louis, MO USA

Question: How do the cells of humans and apes differ when looked at under a
microscope?

---------------------------------------------------------------------------




From daemon Wed Mar 7 23:46:31 2001



From: georgas1-at-home.com
Date: Wed, 7 Mar 2001 23:46:13 -0600
Subject: Ask-A-Microscopist: fractal geometry to diagnose chronic

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(georgas1-at-home.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday, March
7, 2001 at 20:29:09
---------------------------------------------------------------------------

Email: georgas1-at-home.com
Name: Adam Georgas

Organization: UMS-Wright Preparatory School

Education: 9-12th Grade High School

Location: Mobile, Alabama, USA

Question: Dear Microscopist:
I am a 10th grade student working on my science fair project. My project
deals with a computer program I wrote using fractal geometry to diagnose
chronic lymphocytic leukemia. I obtained 25 pictures of normal cells taken
from a Phillips electron microscope and 25 pictures of leukemic cells taken
with the same microscope. I obtained these photographs from my mentor, a
physician/professor at the University of South Alabama. My concern with my
project is that the 25 healthy cells came from only one patient, and the 25
leukemic cells came from another patient. So, I have 50 cell pictures, 25
healthy, 25 leukemic, from only 2 patients. All of the research I have
read use 25 different patients with only one cell from each patient. I am
wondering if my study is valid? I posed this question to a local
pathologist. She told me that since the pictures were taken from an
electorn microscope, it was actually better to have more cells from just 2
patients. Is this true? If it is true, what is the rationale

---------------------------------------------------------------------------




From daemon Thu Mar 8 03:52:10 2001



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Thu, 8 Mar 2001 10:56:26 +0100
Subject: Re: JEOL 2000fxII aberration coefficients

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mark,

Here is some parameters of 2000FX with AHP2OL polepiece:
---------------------------------------------
Point resolution 0.31nm
Lattice resolution 0.14nm
OL focal length 4.1mm
Cs 3.4mm
Cc 3.1mm
---------------------------------------------

As to the question of beam semi-convergence angle, Prof. O'Keef has given a perfect note. I learnt a lot there as well.

Yours,
Shu-You Li
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

Fax: +49-6131-3923768 Tel: +49-6131-3923148(O)
E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
URL: http://syli.homepage.com/
(This URL contains my resume, SCI journal informations, JobList,
TEMAlert, as well as other useful informations related to Transmission
Electron Microscopy)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Thu Mar 8 03:58:50 2001



From: Richard M Langford :      richard.langford-at-materials.oxford.ac.uk
Date: Thu, 8 Mar 2001 09:51:45 -0000
Subject: 3-D reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

Can anyone suggest suitable software for 3-D reconstruction of grains and
cracks from a sequential set of focused ion beam cross-sections.

Regards

Richard
--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273799, Fax: +44 (0)1865 273789
email: richard.langford-at-materials.oxford.ac.uk
----------------------------------------------------------------------------



From daemon Thu Mar 8 06:30:14 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 8 Mar 2001 04:23:52 -0800 (PST)
Subject: Re: I've got the screws loose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula:
Get in touch with Helmut Patzig at MOC, Spring Valley, NY. His email is
Mocleica-at-aol.com. If anybody has the know-how and parts, I am pretty sure
he does. Sorry I don't know of anyone closer to DC, but I have dealt with
Helmut over the years with obsolete Reichert and LKB equipment.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Wed, 07 Mar 2001 08:31:14 -0500, Paula Sicurello wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hi Listers,
|
| I have a LKB Bromma 11800 Pyramitome whose belt has come loos and it
needs to be replaced. I can't find the instruction manual or the slip of
paper that my predecessor wrote the service person's name on.
|
| Does anybody out there have a source for the instruction manual? I
called Leica but they told me this machine was absolete when they acquired
the LKB line.
|
| I'll fix this thing myself if anyone can send me a copy of the manual
(I'll glaly pay for the cost of copying and shipping).
|
| If anyone knows of a repair person who still works on these ancient
beasties, please let me know. It would be good to have someone who could
give them tune-ups every now & then.
|
| My screws are loose and I've got the hammer, blow torch sosme bubble gum
and bailing wire....now all I need is the manual.
|
|
| Pretty please??
|
| Paula :-)
|
| Paula Sicurello
| George Washington Univ. Medical Center
| Dept. of Pathology, Ross Hall rm 505
| Electron Microscope Lab
| 2300 Eye St.
| Washington, DC 20037
| 202-994-2930 phone
| 202-994-2518 fax
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Thu Mar 8 07:07:50 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 8 Mar 2001 06:56:30 -0600
Subject: RE: Question: Resolution and TV Lines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The answer depends on the details of the "TV" system and microscope used.
At this point I don't even know if it is an optical or electron microscope,
etc... It should be noted that actual microscope resolution and the
displayed resolution via TV have little in common.

NTSC (US) TV is 525 lines vertical (total, and the equevelant of 280 to over
640 horizontal (bandwidth dependent). Note that not all 525 lines are used
for the image.

PAL, a standard used in many other areas of the world is slightly higher
vertical resolution, but not much.

There are other "non-brodcast standard" resolutions used by custom
instrumentation.

Woody

} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} }
} }
} } Email: de-at-payload.com
} } Name: De McKeown
} }
} } Organization: Payload Systems
} }
} } Education: Graduate College
} }
} } Location: Cambridge, MA, USA
} }
} } Question: I need to determine the number of TV lines my
} microscope is
} } giving me. I have a test target but it only goes to 266
} l/mm and I have
} } better resolution than that. I can't find a 'better'
} target and am stuck
} } trying to get an answer. How can I do this or where can I
} go for a new
} } target?
} }
} } I also have to find a contrast, but haven't got enough
} information yet to
} } ask a good question.
} }
} } Thank you!!
} }
}
}
}


From daemon Thu Mar 8 07:20:27 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wednesday, March 7, 2001 2:55 PM
Subject: Fwd: TEM - immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Tina,
Check the following reference for your answer to your second question:

Liposits, Zs., D. Sherman, C. Phelix and W.K. Paull. (1986). A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens. Histochemistry 85:95-106.

We very successfully used vibraotomed sections of brain to do light and EM ICC. Becuase of the penetration problems for the gold conjugates available at that time, we used PAP-DAB for visualization on the light level followed by silver intensification of the material for EM visualization. The study involved serial sectioning of the material to locate synapses so flat embedding of the sections was manditory. The method is fully discribed in the paper but feel free to contact me if you have questions.

I would also try using the ultra small gold colloids presently on the market followed by silver intensification as an alternative to the PAP-DAB technique.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907


--------------------------------------


I have two questions for you immunolabeling experts.

First, a client has some precious and irreplaceable histological sections
of human hippocampus that have been labeled with ABC - DAB. Will it be
possible for me to de-paraffinize the sections, expose them to osmium
vapor and re-embed them in resin on the slide and pop them off and
resection them and see the DAB precipitate? Anyone have a protocol that
has worked?

Second, these researchers plan to use Vibratome sections of mouse brain
and immunolabel them for light microscopy using an ABC kit. I have done
on-ultrathin-section colloidal gold immunolabeling for TEM. However, for
this new project we would like to try pre-embedding labeling of 50-60
micrometer Vibratome sections, then embed and resection them for
TEM. Knowing what works for light microscopy, we'd like to use
streptavidin/colloidal gold or whatever for TEM visualization. My question
is, of course, does anyone have a favorite protocol they would be willing
to share? How do I keep the sections from curling? If we stick them on a
glass slide to embed in resin and (hopefully) pop off later for
resectioning, when and how do I get the to adhere?

Mahalo!

Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Thu Mar 8 08:24:10 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 8 Mar 2001 08:17:36 -0600
Subject: Re: IEM high background

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michelle,
The answer to the last question is easy. Remember Bugs Bunny
munching his carrot? Rabbits eat plants so it is not too surprising
that a preimmune serum recognizes a plant antigen, if not several.

What we have done in situations like this is to incubate the
serum with the protein of interest (in this case, the 32kD glucanase)
and then stain with that. This removes the specific glucanse
staining. It is a kind of guilt by subtraction. It is not as nice as
doing affinity purification, but it is a lot faster, and conserves
the serum.

Hope this helps,
Tobias Baskin


} ------------------------------------------------------------------------
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--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
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/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Mar 8 08:24:11 2001



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Thu, 8 Mar 2001 08:18:50 -0600
Subject: CERIUS2: HREM simulation of quantum dots

Contents Retrieved from Microscopy Listserver Archives
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Dear Listservers,
we have been recently working on structural and compositional
characterization of InGaAs/GaAs quantum dots grown by MOVPE, achieving
interesting results.
We have tried to use CERIUS2 to obtain simulations of cross sectional HREM
images of these dots, but we experienced several problems.
Is there anyone who has spent time on similar matters. We would like to
discuss and compare our experiences, to understand if our problems are due
to a bad construction of the supercell or to limitations of the software.
Thanks
Massimo




From daemon Thu Mar 8 08:42:12 2001



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Thu, 08 Mar 2001 10:40:28 -0400
Subject: Re: homemade transmitted electron detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello again listers,

Thanks for all the responses to my original query. At the suggestion
of Bart Cannon, you can check out my detector design at:

http://www.mta.ca/~jehrman/ted.htm

I perhaps should have clarified originally that this is a very simple
design, and does not require any additional electronics, light pipes,
PMT, etc. My machinist made it for me in an afternoon. I took
inspiration
from a single sentence in Goldstein, et. al (SEM and X-ray
Microanalysis,
2nd edition, p. 267):

"A simple, inexpensive detector can be made from a high-atomic-number
scattering surface placed below the specimen and tilted so that the
transmitted electrons are scattered toward the conventional E-T
detector in the specimen chamber"

} From my experience with one commercially available "real" TED a number
of years ago, the homemade detector seems to perform as well (or better)

than the real thing.

No reference was given in the text, so I'm rather curious who came up
with
the idea originally. Are there any co-authors of the text lurking nearby
who
could shed any light?

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Thu Mar 8 11:54:23 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 8 Mar 2001 12:46:00 -0500
Subject: Re: Ask-A-Microscopist: fractal geometry to diagnose chronic

Contents Retrieved from Microscopy Listserver Archives
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Email: georgas1-at-home.com
Name: Adam Georgas

Organization: UMS-Wright Preparatory School

Education: 9-12th Grade High School

Location: Mobile, Alabama, USA

Question: Dear Microscopist:
I am a 10th grade student working on my science fair project. My project
deals with a computer program I wrote using fractal geometry to diagnose
chronic lymphocytic leukemia. I obtained 25 pictures of normal cells taken
from a Phillips electron microscope and 25 pictures of leukemic cells taken
with the same microscope. I obtained these photographs from my mentor, a
physician/professor at the University of South Alabama. My concern with my
project is that the 25 healthy cells came from only one patient, and the 25
leukemic cells came from another patient. So, I have 50 cell pictures, 25
healthy, 25 leukemic, from only 2 patients. All of the research I have
read use 25 different patients with only one cell from each patient. I am
wondering if my study is valid? I posed this question to a local
pathologist. She told me that since the pictures were taken from an
electorn microscope, it was actually better to have more cells from just 2
patients. Is this true? If it is true, what is the rationale

---------------------------------------------------------------------------

Dear Adam,
First, there is nothing about data from an electron microscope that makes
sample selection any different from that used with other forms of data. The
idea is that the population in your sample--in this case the normal and leukemic
cells--should be representative of the larger population--all human normal
lymphocytes and all human leukemic lymphocytes. If all normal lymphocytes in
any one person have identical morphology, then any one cell will represent the
population; if lymphocytes vary within an individual, then you would need a
selection having the same distribution of types as exist in the whole person.
This is usually obtained by selecting a small sample volume "at random". You
might imagine how this could go wrong; e.g., if certain types were not present
in arterial blood to the same extent as in venous blood.
The next consideration is whether either normal lymphocytes or leukemic
lymphocytes differ from person to person. I can see no reason that this would
not be the case--especially if different forms of leukemia result from different
transformation processes; e.g., different mutations or different extents of gene
expression.
In order to be sure that your sample is relevant, you would need to know
whether there were different types of normal and leukemic cells both within and
among individuals. This is an area where I am completely ignorant, but there is
probably an extensive literature on this. There could also be differences
arising from the preparation of the cells for EM, such as variation in fixation
or staining, which can give apparent differences from nomonally identical cells.
Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Thu Mar 8 12:32:37 2001



From: Vu Phan :      vtp_adi-at-yahoo.com
Date: Thu, 8 Mar 2001 10:28:57 -0800 (PST)
Subject: Secondary Electron Detector - ISI 50A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I just started to bring up an ISI-SR50A. Followed the procedure to replace
the filament, saturate beam current and aligment correctly but I could not
get a decent image on the CRT even at low mag, 200x. Is there a way to
check the detector?
I varied the applied voltage from 5KV to 20KV and WD from 7mm to 40mm
without any success.
Any suggestions are welcome.
-Vu.

__________________________________________________
Do You Yahoo!?
Get email at your own domain with Yahoo! Mail.
http://personal.mail.yahoo.com/


From daemon Thu Mar 8 15:16:29 2001



From: thoma226-at-msu.edu
Date: Thu, 8 Mar 2001 15:14:24 -0600
Subject: Ask-A-Microscopist: zooxanthellae from Cnidarians

Contents Retrieved from Microscopy Listserver Archives
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Email: thoma226-at-msu.edu
Name: James Thomas

Organization: Michigan State University

Education: Graduate College

Location: East Lansing, MI USA

Question: How do I attach zooxanthellae from Cnidarians to a stub to view
in SEM? Centrifuging? Fixing? etc?

---------------------------------------------------------------------------




From daemon Thu Mar 8 15:16:30 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 8 Mar 2001 16:12:38 -0500
Subject: top hat filter vs background subtraction for integrated peak inte

Contents Retrieved from Microscopy Listserver Archives
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Here are two questions that I am pondering at the moment for thin film analysis in the TEM.

If you take the integrated intensities of the center portions of the peaks (zero crossing to zero crossing) of a top hat filtered spectrum to use for quantification, will you get the same results as you would if you do a background subtraction and take the integrated intensities from the peaks themselves?

For the integrated intensities of a top hat filtered spectrum, is it more appropriate to include the two negative lobes of the filtered peak added to the center portion?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Thu Mar 8 15:24:09 2001



From: Edward_Principe-at-amat.com
Date: Thu, 8 Mar 2001 13:20:23 -0800
Subject: 3-D reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are two freeware software packages that can accomplish this effort.

An IBM inspired software called OpenDX (for Unix), which is quite advanced.
Start here (I have just installed this software but not worked with it yet):

http://www.opendx.org/

and through scion image, which is a PC version of NIH image. Start at the link
below

http://rsb.info.nih.gov/nih-image/

You must install the ImageJ plugin, which you can obtain from:

http://www.isi.uu.nl/people/michael/vr.htm

This site also has other links for related information.

I am pursuing similar work with FIB/ Auger on particles and I would love to know
about your progress.
I believe this area has interesting and useful applications and I have found
only
very limited publications in this area. I would appreciate any additional
information
you might have.

Good Luck !

Regards,
Ed


************************************************************
Edward Principe, Ph.D.
Member of Technical Staff
Defect & Thin Film Characterization Laboratory
Applied Materials
408-986-3882



"Richard M Langford" {richard.langford-at-materials.oxford.ac.uk} on 03/08/2001
01:51:45 AM


To: microscopy-at-sparc5.microscopy.com
cc: (bcc: Edward Principe/APPLIED MATERIALS)


Dear All

Can anyone suggest suitable software for 3-D reconstruction of grains and
cracks from a sequential set of focused ion beam cross-sections.

Regards

Richard
--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273799, Fax: +44 (0)1865 273789
email: richard.langford-at-materials.oxford.ac.uk
----------------------------------------------------------------------------






From daemon Thu Mar 8 16:48:49 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 8 Mar 2001 16:50:32 -0600
Subject: TEM: cholesterol crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Richard,

If you only need to do reconstruction, download our free VoxBlast Light
software at www.vaytek.com. Go to VoxBlast 3D software, then VoxBlast Light
3.0.

If you need any help or want more functionality, feel free to contact us
directly.


Best regards,

Steve Niemela
Sales
sniemela-at-vaytek.com
Ph: 641-472-2227
Fax: 641-472-8131

VayTek, Inc.
305 West Lowe Avenue
Fairfield IA 52556
www.vaytek.com
----- Original Message -----
} From: "Richard M Langford" {richard.langford-at-materials.oxford.ac.uk}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 08, 2001 3:51 AM


A colleague is looking for a published reference that describes
cholesterol crystals using transmission electron microscopy. We have
searched the web but found only light microscopy of the crystals. Any
help would be appreciated.

Thank you,

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Mar 8 17:14:23 2001



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 8 Mar 2001 18:11:04 -0500
Subject: Job posting-Flow Cytometry/Microscopy Facility Scientist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Facility Scientist (Academic Assistant III)
Flow Cytometry and Confocal Imaging Facility, Biotechnology Center

The Biotechnology Center and the Department of Molecular and Cell
Biology at the University of Connecticut invite applications for the
position of Facility Scientist for the Flow Cytometry and Confocal
Imaging Facility. This Facility houses a Becton Dickson FACSCalibur
Flow Cytometer/cell sorter, a Leica SP2 laser scanning confocal
microscope and several other microscope and image processing
workstations. The facility scientist will be responsible for working
with University faculty and students to develop research projects
that utilize these instruments and related technologies. The
facility scientist will also organize periodic training sessions and
workshops to familiarize new users with the instruments. This
individual will also be encouraged to develop collaborative research
projects with University faculty and with local biotechnology
businesses. We seek an individual who is excited by the challenges
of an interdisciplinary research group and who enjoys interacting
with people in an active scientific environment. The preferred
candidate will have a Ph.D. in the biological sciences and
familiarity with one or both of the core technologies. This is a
full-time, annually renewable position. Salary will be commensurate
with experience and education. Submit curriculum vitae, a statement
of experience and interests, and the names, addresses, telephone
numbers and e-mail addresses of at least three references to:
Biotechnology Center, Confocal Search Committee, University of
Connecticut, 184 Auditorium Rd., Unit 3149, Storrs, CT 06269-3149.
Applications may also be faxed to (860) 486-5005 or sent
electronically to biotctr1-at-uconnvm.uconn.edu. Screening of
applications will begin immediately and continue until the position
is filled.

We encourage applications from under-represented groups including
minorities, women and people with disabilities. (Search #01A372)
--

************************************************************
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269-3125
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************


From daemon Thu Mar 8 17:47:13 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Thu, 08 Mar 2001 15:43:36 -0800
Subject: jet electropolisher fire

Contents Retrieved from Microscopy Listserver Archives
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The following posting is for a colleague who is not on the listserver.

Larry Thomas
Pacific Northwest National Laboratory
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto:Larry.Thomas-at-pnl.gov


We recently experienced a fire using a 5% perchloric acid - methanol electrolyte
in a commercial jet electropolisher, and request information on similar
experiences.

During operation of the electropolishing unit at room temperature and moderate
pump speed, when turning up the voltage towards 30 V a loud pop was heard and
the reservoir was found to be in flames. The fire was quickly extinguished
using a dry fire extinguisher, but the reservoir and cable shielding melted.

We would appreciate any input on similar experiences using electropolishers with
this or similar electrolytes. Any explanations for this behavior that you can
provide would also be appreciated.

David S. Gelles
Structural Materials Research
Pacific Northwest National Laboratory
Richland, WA 99353
Tel: (509) 376-3141
Fax: (509) 376-0418
E-mail: ds_gelles-at-pnl.gov



From daemon Thu Mar 8 19:59:02 2001



From: Frederick Schamber :      fhscham-at-stargate.net
Date: Thu, 08 Mar 2001 21:07:01 -0500
Subject: Re: top hat filter vs background subtraction for integrated peak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are two ways that the top-hat filter has been used for quantifying EDS spectra:

(1) One can simply apply the filter to the spectrum as you suggest -- what results looks much like the second derivative of the original spectrum and each peak is represented by a central positive lobe with a negative lobe on either side. The slowly varying (continuum) components of the spectrum are
suppressed. With care, one can relate the area of the positive lobe to the area of the original peak, however, there are some issues which make this simple concept a less than satisfactory quantitative method: (1) the area of the positive lobe is proportional to the area of the original peak, but the
proportionality is a function of both peak area and filter shape. Thus the proportionality varies with the peak energy since the peak width varies with energy. (2) overlapping and adjacent peaks will interfere with each other, and this interference is actually increased by the broadening effect of the
filter. Oddly enough, I have seen a number of familiar reference works which seem to give me credit for inventing the "top hat filter" used in this way -- I didn't. I saw it used this way for nuclear gamma ray spectra in the early '70s and after trying it myself, realized that it really wasn't
satisfactory. But this experience did lead to the "filter-fit" method which follows.

(2) The"right" way for using the top-hat filter for continuum suppression is to use it in conjunction with linear-least squares fitting. One simply filters the unknown and each of the reference spectra to be fitted to it and then applies a conventional linear fit between them. When one fits the filtered
references to the filtered unknown, the fact that the spectra have been distorted by the filter is immaterial (since the filter operator is linear), the overlaps are accurately unfolded, and the continuum basically drops out of the problem. Properly implemented, this is a very good quantitiative technique
and has been employed productively for almost 30 years. If interested, the technique is published in NBS 604 (P273) or interested parties could contact me for references to several other papers describing it.

Fred Schamber


"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Here are two questions that I am pondering at the moment for thin film analysis in the TEM.
}
} If you take the integrated intensities of the center portions of the peaks (zero crossing to zero crossing) of a top hat filtered spectrum to use for quantification, will you get the same results as you would if you do a background subtraction and take the integrated intensities from the peaks themselves?
}
} For the integrated intensities of a top hat filtered spectrum, is it more appropriate to include the two negative lobes of the filtered peak added to the center portion?
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)



From daemon Thu Mar 8 20:59:35 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 9 Mar 2001 13:56:15 +1000
Subject: re: TEM - immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tina,

I've been gold immunolabelling whole retina pieces - 300um thick
sheets - with great success (LM and EM) for years. I react the tissue
in small vials, floating around loose. Briefly, you need a fixative
like PLP or paraformaldehyde - any glut in the fix and antibody
access to the tissue is decreased and you just get labelling on the
cut edges. Use saponin in all solutions for penetration enhancement.
Use an Fab2 anti Ig conjugated to 1 nm gold. Silver enhance then gold
tone. Pop tissue in a silicon mould for embedding. If this sounds
useful I can send you the full method.

As for rescuing paraffin sections, I did some a long time ago. From
memory the resulting tissue looked like squashed newspaper - a mushy
black mess with a hint of structure. I can't imagine even being able
to distinguish the DAB from the rest, let alone see what was
labelling. However, maybe methods have improved.

Cheers,

Diana
--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Fri Mar 9 03:47:39 2001



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Fri, 09 Mar 2001 09:42:22 +0000
Subject: Re: jet electropolisher fire

Contents Retrieved from Microscopy Listserver Archives
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In my opinion what happened was that a spark between the cathode and specimen, which
often occurs at those voltages during electropolishing, ignited the electrolyte. I
have used other electrolytes at voltages greater than 30V and I have also head a
crackling noise which indicated to me that was sparking. Fortunately the electrolyte
was not as ignitable as a 5% perchloric acid - methanol electrolyte. Nevertheless, I
kept the voltage below that just in case for the electrolyte was still based on
organic solvents.

You are going to be bombarded by horror stories and cautions about electrolytes
containing perchloric acid. And they are right. I will not say that I have used
electrolytes containing up to 30 vol.% in ethanol without problems because that
would be tempting fate and I have enough Sicilian blood in me to make me a bit
superstitious.

I have some recommendations.

1. As far as I know, perchloric acid electrolytes only require a voltage of 15V max.
It is rare that I have used voltages in excess of 25V for any electrolyte and that
was with an electrolyte of methanol, Butoxy-ethanol, magnesium perchlorate and
lithium chloride (LiCl). This was the electrolyte where above 30V I was getting
sparking. I have used water based electrolytes above 30V sometimes, but ignition is
not an issue there.

2. Whenever making up or using an electrolyte containing perchloric acid, cool it
down. When making it up, this prevents ignition due to heat of dissolution of the
concentrate acid as it mixes. This may be paranoia. Cooling during electropolishing
is necessary because it also reduces the risk if ignition and it improves the
electropolishing process. I have found that most organic based electrolytes,
especially those based on ethanol and methanol require cooling to {20°C in order to
achieve the correct electropolishing conditions. So the necessity of cooling is two
fold.

I hope this is useful.


"Thomas, Larry (PNNL)" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} The following posting is for a colleague who is not on the listserver.
}
} Larry Thomas
} Pacific Northwest National Laboratory
} Richland, WA 99352
} Phone: (509)372-0793 Fax: (509)376-6308
} Email: mailto:Larry.Thomas-at-pnl.gov
}
} We recently experienced a fire using a 5% perchloric acid - methanol electrolyte
} in a commercial jet electropolisher, and request information on similar
} experiences.
}
} During operation of the electropolishing unit at room temperature and moderate
} pump speed, when turning up the voltage towards 30 V a loud pop was heard and
} the reservoir was found to be in flames. The fire was quickly extinguished
} using a dry fire extinguisher, but the reservoir and cable shielding melted.
}
} We would appreciate any input on similar experiences using electropolishers with
} this or similar electrolytes. Any explanations for this behavior that you can
} provide would also be appreciated.
}
} David S. Gelles
} Structural Materials Research
} Pacific Northwest National Laboratory
} Richland, WA 99353
} Tel: (509) 376-3141
} Fax: (509) 376-0418
} E-mail: ds_gelles-at-pnl.gov

--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Fri Mar 9 06:14:57 2001



From: Diego :      diegoalv-at-incar.csic.es
Date: Fri, 9 Mar 2001 14:42:18 +0100
Subject: Re: Secondary Electron Detector - ISI 50A

Contents Retrieved from Microscopy Listserver Archives
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I don´t know about the ISI50A but, assuming that it has the typical
Everhard-Thornley design, isn´t it just possible that the bias voltage is
inverted in your detector?. If the bias voltage is set negative, it´ll be
rejecting all the secondary electrons, and you would only get a generally
noisy image composed of the primary electrons backscattered in the very
direction of the detector´s position. Quite nice for topographic information
about flat specimens, but far worse quality than in "normal" SE images.
Pardon me if I raised a too obvious explanation, but it occurred to me
sometimes to get puzzled with a bad functioning of my SE detector just
because another user of the microscope had been playing with the knobs.

Cheers

Diego


Diego Alvarez
Instituto Nacional del Carbón
INCAR-CSIC
Spain



From daemon Fri Mar 9 07:42:00 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 09 Mar 2001 08:36:17 -0500
Subject: My pyramitome has many heroes

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,

Thanks to all who responded to may plea for help in finding either a manual or a service person for the wee beastie.

Slowly but surely I will tighten the screws.....


Thanks again!


Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Mar 9 08:26:51 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 9 Mar 2001 15:22:37 +0100
Subject: TNT2k1

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Full details of Trends in Nanotechnology (TNT) 2001, Segovia, Spain,
September 2001, are now available. Based on the same format as last year,
the conference aims to bring together researchers from all disciplines
relating to nanotechnology, in a setting with ample time for interaction. A
new feature this year will be the availability of grants to enable graduate
students to attend and present posters.

Full details can be found at www.cmp-cientifica.com/tnt2001

Regards

Tim

*****************************************************************
Tim E. Harper CEO
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/




From daemon Fri Mar 9 09:03:41 2001



From: Eric Steel :      eric.steel-at-nist.gov
Date: Fri, 09 Mar 2001 09:58:36 -0500
Subject: Re: analysis of EDS X-ray spectra

Contents Retrieved from Microscopy Listserver Archives
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The NIST's web link to DTSA can be found at:
http://www.cstl.nist.gov/div837/837.02/MicroscopySoftware.html

This program is free and available for the Macintosh only.


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-417-1321
100 Bureau Drive, Stop 8371
Gaithersburg, MD 20899-8371
http://www.nist.gov/cstl/div837/837.02/


From daemon Fri Mar 9 10:29:52 2001



From: Edmund Gierlik :      gierlik-at-delta.sggw.waw.pl
Date: Fri, 09 Mar 2001 17:24:27 +0100
Subject: ESEM-Need presentation of phase transition.

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Email: gierlik-at-delta.sggw.waw.pl
Name: Edmund Gierlik

Organization: Electron Microscopy Lab.

Education: professor

Location: Warsaw, Poland

Question: Can anybody show the source of nice presentation of ESEM
application in
a phase transition investigation (water - ice, liquid - solid)?


From daemon Fri Mar 9 15:05:07 2001



From: Ellen S. Morgan :      emorgan-at-caregroup.harvard.edu
Date: Fri, 9 Mar 2001 16:02:53 -0500
Subject: Formvar Coated grids

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Any advice for a novice about using formvar coated slot grids for
biological Epon sections? Thanks


From daemon Sun Mar 11 13:43:54 2001



From: Ellen S. Morgan :      emorgan-at-caregroup.harvard.edu
Date: Sun, 11 Mar 2001 14:31:50 -0500
Subject: Using Formvar Grids

Contents Retrieved from Microscopy Listserver Archives
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TO be more specific, any advice on using formvar coated slot grids for
serial sections. They seem to crinkle, or get blobby, right on the vessel
I want to look at.


From daemon Mon Mar 12 06:51:53 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 12 Mar 2001 04:36:11 -0800 (PST)
Subject: Re: Using Formvar Grids

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Ellen:
Another question: are you using bare formvar or have you coated with
carbon? Bare formvar will pucker, but the addition of a little carbon does
wonders for stabilization and strength of the films. My experience is using
0.5% formvar, stabilized with about 10nm of evaporated carbon. These films
aren't too thick, allowing good image resolution at 80kV. BTW: if the
formvar films are puckered, they will show it prior to picking up sections.
(Another vagrant neuron just fired.) One way to deal with the puckered
formvar is to use chloroform or carbon tet. Simply pass the grid over the
mouth of an open bottle, or, as a last resort, put the grid into the neck of
the bottle (NOT into the fluid--just the vapors). Please be careful with
this. (Exposure to these chemicals.) I may have the beginnings of
interstitial pulmonary fibrosis--a not uncommon result of exposure to
chemicals.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Sun, 11 Mar 2001 14:31:50 -0500, Ellen S. Morgan wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| TO be more specific, any advice on using formvar coated slot grids for
| serial sections. They seem to crinkle, or get blobby, right on the
vessel
| I want to look at.
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon Mar 12 07:18:25 2001



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Mon, 12 Mar 2001 08:18:27 -0800
Subject: buildings with low magnetic fields

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Fellow microscopists,

Does anyone know of any buildings/laboratories recently constructed that
house instruments (not necessarily EM's) requiring very low ( {0.5 mG) 60 Hz
stray magnetic fields? I am interested in contacting someone (scientist,
building manager, architect, etc.) who might know the details of how that
building was constructed.

Thank you for your time and help,

Sincerely,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu


From daemon Mon Mar 12 08:37:37 2001



From: Leslie Eibest :      leibest-at-duke.edu
Date: Mon, 12 Mar 2001 09:32:41 -0500
Subject: ESEM-Need presentation of phase transition.

Contents Retrieved from Microscopy Listserver Archives
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At 5:24 PM +0100 3/9/01, Edmund Gierlik wrote:
} Email: gierlik-at-delta.sggw.waw.pl
} Name: Edmund Gierlik
} Organization: Electron Microscopy Lab.
} Education: professor
} Location: Warsaw, Poland
}
} Question: Can anybody show the source of nice presentation of ESEM
} application in a phase transition investigation (water - ice, liquid - solid)?

Trisha Rice at the ESEM applications lab might be able to
provide this. She can be reached at either trice-at-feico.com or
trice-at-electroscan.com (I'm not sure if this last address is still
valid).

Leslie Eibest


From daemon Mon Mar 12 08:45:19 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 12 Mar 2001 09:41:50 -0500
Subject: Re: TEM: Focusing

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Dear Randy,
I have just a few points to add to Steve's excellent advice.

1. "It is too dim to focus" - then
i) increase the emission current, many people run at too low a
current and make the task of optimising the instrument settings much more
difficult
ii) increase the kV which would increase the intensity too
ii) re calibrate the photographic system to allow a focus intensity
that is suitable for most operators

I often use LoDose film, which is more than an order of magnitude more
sensitive than SO163, so I can barely see the beam on the screen. Fortunately,
we have an intensified CCD, which is so sensitive that I can still focus. In
fact, using the minimum-contrast method works very well with this system, since
the contrast can be electronically enhanced.

6. To focus at low magnifications try removing the objective aperture
and focus without a binocular, or a wobbler, looking for the very strong
minimum contrast effect.

Furthermore, I discovered that inserting the diffraction aperture (with the
objective aperture out) allows this method to be used at up to 63kx (and
possibly higher). Since minimum contrast can easily be found within one second,
this is well suited to radiolabile specimens.

Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Mon Mar 12 09:02:31 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 12 Mar 2001 10:06:35 -0500
Subject: Re: Using Formvar Grids

Contents Retrieved from Microscopy Listserver Archives
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This is a different procedure from the Formvar-coated grids that you are using
but it's one of my favorite journal articles/techniques - J. C. Rowley and
D.T. Moran, A Simple Procedure for Mounting Wrinkle-Free Sections on
Formvar-Coated Slot Grids. Ultramicrotomy 1 (1975) 151-155. They use
Formvar-coated aluminum supporting racks. We use this method for slot grids
all the time rather than Formvar-coated grids.
good luck,
Beth
} TO be more specific, any advice on using formvar coated slot grids for
} serial sections. They seem to crinkle, or get blobby, right on the vessel
} I want to look at.

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Mon Mar 12 09:38:08 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Mon, 12 Mar 2001 10:15:57 -0500
Subject: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
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Dear Ellen:

I do quite a bit of serial sectioning and would like to offer you the
following advice:

1) Cut your sections as small as possible, they can be as much as 1mm wide,
but try to make them something like 0.1mm high. This will allow you to get
you as many as 20 sections on a 2mm slotted grid.

2) Get a bunch of locking tweezers and from the time you pick up the grid
until you put it in the microscope, do not put the grid down onto filter
paper (as you would for a normal grid). So, after you pick up the ribbon of
sections, leave the grid in the tweezers to dry, and then when you post
stain the grids, leave them in the tweezers to dry. If you put them down
onto filter paper the formvar may rupture.

3) Maintain a positive attitude, and if things aren't going your way, stop
and come back to it another day (the blocks will always be there for you,
but if you get burned out you won't be there for the blocks).

Best of Luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Mon Mar 12 09:58:42 2001



From: Richard Leapman :      leapman-at-helix.nih.gov
Date: Mon, 12 Mar 2001 11:00:24 -0400
Subject: Biologist/Technician position available at NIH

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*** BIOLOGIST POSITION AVAILABLE ***

NATIONAL INSTITUTES OF HEALTH
Bethesda, Maryland
Division of Bioengineering & Physical Science
Supramolecular Structure & Function Lab.

Biologist GS9/GS11/GS12 trained in life sciences and/or physical sciences
at BS or MS level with solid technical experience in thin-section
transmission electron microscopy. Experience in advanced techniques in
analytical electron microscopy and structural biology (cryosectioning,
high-pressure freezing, and macromolecular preparation methods) is
desirable, although on-the-job training is possible for an experienced
microscopist. PROOF OF U.S. CITIZENSHIP IS REQUIRED for this appointment.


For further information please contact:

Dr. Richard Leapman
Division of Bioengineering & Physical Science
Bldg. 13, Rm. 3N17
National Institutes of Health
Bethesda, MD 20892
Tel: (301) 496-2599
FAX: (301) 496-6608
E-mail: leapman-at-helix.nih.gov


Applications (with curriculum vitae or federal employment form SF-171)
should include the reference number ORS-01-0044, and should be post marked
by April 9, 2001 and sent to:

Attention: Mr. Harold Atkins
National Institutes of Health
ORS Human Resources Office
31 Center Drive Msc 2157
Bldg 31, Room 4b41
Bethesda
MD 20892-2157

E-mail: orspersonnel-at-mail.nih.gov
Phone: 301-496-5623
FAX: 301-402-1057

Detailed information about the position is available at

http://careerhere.nih.gov/CHPublic/HRShowVac.taf?&VACANCY_uid1=7257







From daemon Mon Mar 12 10:10:27 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 12 Mar 2001 10:04:38 -0600
Subject: TEM:Focusing

Contents Retrieved from Microscopy Listserver Archives
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Many thanks to everyone who responded to my question about focusing.
Although my actual question was answered almost immediately, the ensuing
replies were very enlightening. In EM work, it seems, even the "simplest"
things have dimensions that aren't always obvious.

My initial question arose from the fact that I was curious about something I
had always done as a matter of course because I had been taught to do it
that way. That is, I have spent years focusing TEM's with the beam at the
crossover point because I believed that I'd been taught to do so because of
some property of electron "optics" that made it the preferred method to
ensure best focus. Turns out, though, that the reason was something else
entirely, involving an earlier generation of microscopes that simply had a
beam that was too dim for focusing at the intensity needed for recording an
image.

It's always educational to question old assumptions. Embarrassing, too,
sometimes....

Thanks again, listers.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Mon Mar 12 11:19:17 2001



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 12 Mar 2001 12:35:37 -0500
Subject: Re: TEM - immunolabeling

Contents Retrieved from Microscopy Listserver Archives
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Dear Tina:

Technically, re-embedding paraffin sections for EM is perfectly
feasible. The way I have done it is to separate the sections from slides
after re-hydration and before further fixation with glutaraldehyde and
osmium. Since both glutaraldehyde and osmium treatment cause sections to
shrink and be brittle, sections might get further torn if they are still
attached to the slides. Another step you should be careful about is to keep
sections flat when applying glutaraldehyde and osmium. This ensures
sections not to curl or wrinkle later. You can float your sections in 0.1 M
phosphate buffer in a Petri dish, and let sections lie flat on the bottom.
Then you gently remove buffer and add fixative with a glass pipet without
disturbing the sections. Do not shake sections for a while after adding
fixative. To flat-embed the sections, you can sandwich sections that have
been fully infiltrated with resin between two sheets of Aclar film, then
place this sandwich between two glass slides and apply some weight on top
while polymerizing the resin.
As someone already pointed out, even though you can re-embed paraffin
sections for EM, ultrastructural will not be adequate anymore. This is
particularly a problem for examining brain tissue in which membrane outline
is essential for identifying different neuronal elements. However, if the
tissue is precious, I would say it is still worth trying. We work with
post-mortem brain tissue often. Sometimes the brains were over twenty-four
hours post-mortem, fixed with only formalin and stored in cryoprotectant in
a freezer for a long time. I am often surprised how much information we can
still obtain from such tissue at EM level. I would be happy to help you
to evaluate your micrographs if you like.

Good luck, Tina, let me know if you need more detailed answers.

Hong

==============
Hong Yi
Supervisor
Emory University School of Medicine
Neurology Microscopy Core Laboratory
Rm 6215 Woodruff Memorial Research Building
1639 Pierce Dr.
Atlanta, GA 30322
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


----------
} From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
} Subject: TEM - immunolabeling
} Date: Wed, Mar 7, 2001, 2:55 PM
}

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have two questions for you immunolabeling experts.
}
} First, a client has some precious and irreplaceable histological sections
} of human hippocampus that have been labeled with ABC - DAB. Will it be
} possible for me to de-paraffinize the sections, expose them to osmium
} vapor and re-embed them in resin on the slide and pop them off and
} resection them and see the DAB precipitate? Anyone have a protocol that
} has worked?
}
} Second, these researchers plan to use Vibratome sections of mouse brain
} and immunolabel them for light microscopy using an ABC kit. I have done
} on-ultrathin-section colloidal gold immunolabeling for TEM. However, for
} this new project we would like to try pre-embedding labeling of 50-60
} micrometer Vibratome sections, then embed and resection them for
} TEM. Knowing what works for light microscopy, we'd like to use
} streptavidin/colloidal gold or whatever for TEM visualization. My question
} is, of course, does anyone have a favorite protocol they would be willing
} to share? How do I keep the sections from curling? If we stick them on a
} glass slide to embed in resin and (hopefully) pop off later for
} resectioning, when and how do I get the to adhere?
}
} Mahalo!
}
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}


From daemon Mon Mar 12 11:33:20 2001



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 12 Mar 2001 12:52:18 -0500
Subject: Re: TEM - immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina:

Regarding your question about pre-embedding immunolabeling of vibratomed
brain sections, the type of labeling to use depends on the questions the
researchers are trying to answer. Immunoperoxidase gives greater labeling
depth, but limited spatial resolution. If they simply want to know what
neuronal elements are labeled, then immunoperoxidase is fine. Otherwise,
imunogold should be considered because it provides much more precise
localization.

The methods for pre-embedding immunogold labeling using vibratomed brain
sections have been very well documented. One of the first publications was
by Chan, Aoki and Pickel (1) at Cornell University in 1990, shortly after
ultrasmall gold conjugates were introduced. Many brain researchers have
adapted their protocol. The characteristics of this protocol are the use of
acrolein in the fixative for perfusion, using little or no detergent
treatment for permeabilization and using a 1 nm colloidal gold conjugated
IgG secondary probe in conjunction with a light insensitive, low viscosity,
but fast reacting silver enhancement reagent. I have seen many beautiful
results using this protocol. However, the particle size tends to be big,
and irregular in shape. The cause of this is mainly in the silver
enhancement reagent. In 1999, a new EM grade silver enhancement reagent was
introduced to the market. Because of the improved enhancement homogeneity in
particle size and shape, this silver enhancement reagent allowed us to
conduct for the first time the pre-embedding double immunogold labeling in
brain tissue using only ultrasmall gold conjugates (2).

To avoid a lengthy posting, I will send you the protocol, along with
images off-line.

1. Chan J. Aoki C. Pickel VM. (1990) Optimization of differential
immunogold-silver and peroxidase labeling with maintenance of ultrastructure
in brain sections before plastic embedding. Journal of Neuroscience Methods.
33(2-3):113-27,

2. Hong Yi, Jan L.M. Leunissen, Ge-Ming Shi, Claire-Anne Gutekunst, and
Steven M. Hersch (2001) A Novel Procedure for Pre-embedding Double
ImmunogoldSilver Labeling at the Ultrastructural Level J. Histochem.
Cytochem. 2001 49: 279-284.

Hong


==============
Hong Yi
Supervisor
Emory University School of Medicine
Neurology Microscopy Core Laboratory
Rm 6215 Woodruff Memorial Research Building
1639 Pierce Dr.
Atlanta, GA 30322
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


----------
} From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
} Subject: TEM - immunolabeling
} Date: Wed, Mar 7, 2001, 2:55 PM
}

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have two questions for you immunolabeling experts.
}
} First, a client has some precious and irreplaceable histological sections
} of human hippocampus that have been labeled with ABC - DAB. Will it be
} possible for me to de-paraffinize the sections, expose them to osmium
} vapor and re-embed them in resin on the slide and pop them off and
} resection them and see the DAB precipitate? Anyone have a protocol that
} has worked?
}
} Second, these researchers plan to use Vibratome sections of mouse brain
} and immunolabel them for light microscopy using an ABC kit. I have done
} on-ultrathin-section colloidal gold immunolabeling for TEM. However, for
} this new project we would like to try pre-embedding labeling of 50-60
} micrometer Vibratome sections, then embed and resection them for
} TEM. Knowing what works for light microscopy, we'd like to use
} streptavidin/colloidal gold or whatever for TEM visualization. My question
} is, of course, does anyone have a favorite protocol they would be willing
} to share? How do I keep the sections from curling? If we stick them on a
} glass slide to embed in resin and (hopefully) pop off later for
} resectioning, when and how do I get the to adhere?
}
} Mahalo!
}
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}


From daemon Mon Mar 12 13:03:37 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 12 Mar 2001 12:58:48 -0600
Subject: Agarose enrobed cells

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


A user has encountered an interesting problem . When cells are scraped and
pelleted and embedded directly in epoxy they appear OK under the scope. When
they are enrobed in agarose and then embedded the appearance becomes variable,
although most cells appear very dense with contrast between organelles very low.
This variability in appearance can be on the same grid square.
Any ideas?
Thanks
Bruce
Bruce Cutler
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas





From daemon Mon Mar 12 13:48:58 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 12 Mar 2001 13:45:29 -0600
Subject: ESEM-Need presentation of phase transition.

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"'Microscopy-at-sparc5.microscopy.com '" {Microscopy-at-sparc5.microscopy.com}


May be it's not really a phase transition, but you can
find pictures of dissolution and crystallization of
table salt at our web page:
http://www.umkc.edu/dentistry/microscopy/Esemimg.htm

Vladimir

-----Original Message-----
} From: Edmund Gierlik
To: Microscopy-at-sparc5.microscopy.com
Sent: 3/9/01 10:24 AM



Email: gierlik-at-delta.sggw.waw.pl
Name: Edmund Gierlik

Organization: Electron Microscopy Lab.

Education: professor

Location: Warsaw, Poland

Question: Can anybody show the source of nice presentation of ESEM
application in
a phase transition investigation (water - ice, liquid - solid)?


From daemon Mon Mar 12 14:10:50 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 12 Mar 2001 15:05:41 -0500
Subject: TEM on cellulose and iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
I have a customer who wants TEM on a section of cellulose that is coated
with a layer of Fe2. I have no idea about how to go about accomplishing
this. He wants to see where the Fe2 is located in the cellulose matrix,
and how deep it has penetrated. The sample is a layer of cellulose about
1/2 mm thick with a visible layer of Fe on the surface and it's in
submerged in water.
Any ideas out there?
Thanks so much,

Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-5700


From daemon Mon Mar 12 14:16:06 2001



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 12 Mar 2001 16:15:20 -0400
Subject: schematic for transmitted electron detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yo listers,

After several requests, I've posted a measured schematic of my
transmitted electron adapter, which can be reached via a link
from the page mentioned previously:

http://www.mta.ca/~jehrman/ted.htm

I had the adapter made of aluminum and brass (just because
the machinist likes to work with these). The angled surface
below the specimen grid is polished brass, and as mentioned
by several respondents, could be plated/coated/covered with
something that generates more secondaries. Signal, however,
has not been a problem in our applications - more than enough
even at the smallest spot sizes.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Mon Mar 12 16:01:30 2001



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Mon, 12 Mar 2001 16:55:53 -0500
Subject: LKB microtome repair

Contents Retrieved from Microscopy Listserver Archives
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I have a LKB Ultrotome V which has given many years of faithful
service to me and my students until today, when the band that raises
and lowers the cutting arm snapped. I am in the middle of the
semester class on EM techniques and hence am a bit desperate to get
it repaired. Does anyone know where I might find a replacement part
for this - and is it possible to replace and adjust it myself? There
are no instructions in my manual for this procedure; are there any
out there somewhere? Is there any company that will service this
instrument? I am located in New England.

Many thanks in advance for any leads.

Sincerely,

Dick Briggs
Biology Department
Smith College
Northampton, MA 01063


From daemon Mon Mar 12 17:59:55 2001



From: Susan Belfry :      belfry-at-unb.ca
Date: Mon, 12 Mar 2001 19:55:17 -0400
Subject: MSC2001 Conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This notice is to invite you to attend and participate in the 28th Annual
Meeting of the Microscopical Society of Canada. This three day meeting
will be held at the University of New Brunswick, Fredericton, New
Brunswick, Canada on 6-8 June, 2001.
The Scientific Program features invited speakers from the environmental
sciences, plant sciences, material sciences, EELS applications, confocal
microscopy and magnetic resonance imaging (MRI). Workshops will focus on
specimen preparation techniques for both the biological and material
sciences, including ultramicrotomy, polishing methods, high-pressure
freezing techniques and a workshop on preparation of digital images for
publication. Vendors and manufacturers will be demonstrating the latest
developments in the field of microscopy.
Presentations can be in either platform or poster format and the Abstract
Deadline is March 30, 2001. The deadline for Pre-registration is April 30,
2001. All information and required forms for registration and submission
of abstracts are available at the conference website at:
http://www.unb.ca/msc2001.
************
Keynote Speakers and topics include:
Dr. Bruce Balcom, Department of Physics, University of New
Brunswick. Title of talk: "Magnetic Resonance Imaging of Materials".
Dr. Ron Gronsky, Materials Science and Engineering, University of
California, Berkeley. Title of talk: TBA.
Dr. Michael Hochella, Dept. of Geological Sciences, Virginia Polytechnic
Institute & State University, Blacksburg, Virginia. Title of talk:
"Contributions of Microscopy to the Environmental Sciences: From Bacteria
to Atoms"
Dr. Richard Howard, DuPont Agricultural Products, Experimental Station,
Wilmington, Delaware. Title of talk: "Trends in imaging fungal pathogens
for understanding the cell biology of plant disease"
Dr. Peter Ottensmeyer, Medical Biophysics, University of Toronto. Title of
talk: "3-D Reconstruction of Macromolecules from single-particle STEM
images: Transmembrane signalling of the insulin receptor"

Invited Speakers include:
Mr. Jason Davis, Medical Biophysics, University of Toronto. Title of Talk:
"EM of Chromophores: Very Low Energy-Loss Imaging of Green Fluorescent
Protein and other Coloured Denizens."
Dr. Gianluigi Botton, Materials Technology Laboratory, Natural Resources
Canada. Title of Talk: "Analytical TEM of Macrostructures and Nanostructures".
Dr. Elaine Humphrey, Biosciences EM Facility, University of British
Columbia. Topic: "Confocal Microscopy"
Dr. John McCaffrey, Institute for Microstructural Sciences, National
Research Council Canada. Title of talk: "Structural Characterisation of
InAs/GaAs and InAs/InP Quantum Dots by TEM"
Dr. Doug Perovic, Dept. of Metallurgy & Materials Science, University of
Toronto. Topic: "EM of Semiconductors".
************
Fredericton, New Brunswick is a three hour drive north from
Bangor, Maine and an eight hour drive from Boston or Montreal. June is a
beautiful time in New Brunswick and you can find interesting things to do
and see through the conference website tourism links.



From daemon Tue Mar 13 02:14:16 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 13 Mar 2001 08:07:13 +0000 (GMT Standard Time)
Subject: Re: RE: ESEM-Need presentation of phase transition.

Contents Retrieved from Microscopy Listserver Archives
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We turned water to ice on plant surfaces using the Peltier
stage. We did not take any nice pictures (I have a few
ugly ones!). It was an idea re ice nucleation on plants
for a student project idea that we did not follow up.

Dave


On Mon, 12 Mar 2001 13:45:29 -0600 "Dusevich, Vladimir"
{DusevichV-at-umkc.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} May be it's not really a phase transition, but you can
} find pictures of dissolution and crystallization of
} table salt at our web page:
} http://www.umkc.edu/dentistry/microscopy/Esemimg.htm
}
} Vladimir
}
} -----Original Message-----
} } From: Edmund Gierlik
} To: Microscopy-at-sparc5.microscopy.com
} Sent: 3/9/01 10:24 AM
} Subject: ESEM-Need presentation of phase transition.
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Email: gierlik-at-delta.sggw.waw.pl
} Name: Edmund Gierlik
}
} Organization: Electron Microscopy Lab.
}
} Education: professor
}
} Location: Warsaw, Poland
}
} Question: Can anybody show the source of nice presentation of ESEM
} application in
} a phase transition investigation (water - ice, liquid - solid)?
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Mar 13 03:51:48 2001



From: Dmitri Lapotko :      Ld-at-hmti.ac.by
Date: Tue, 13 Mar 2001 11:54:50 +0300
Subject: LM live cell preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Group,

I need to fix live cells (mainly blood cells) on a microscope glass
slide to prevent them from movement but to maintain their
viability for 1-3 hrs. Also it should be non-stained with any dye
cells in mono-layer.
Material to fix cells should be optically transparent in 500-700 nm.
Room temperature is asssumed.

Any input about applicable technologies (gels etc) with references
about suppliers of materials that are involved and sources for description
of those techniques will be highly appreciated.

Thanks in advance

Dmitri Lapotko, Ph.D.

Luikov Heat and Mass Transfer Institute
15, Brovka Street
Minsk, 220072
Belarus

Tel: (375172)842483
Fax: (375172)842486
ld-at-hmti.ac.by




From daemon Tue Mar 13 06:25:12 2001



From: David Knecht :      knecht-at-uconn.edu
Date: Tue, 13 Mar 2001 09:27:18 -0500
Subject: Ca ratio imaging and arc source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Patton, David" {David.Patton-at-uwe.ac.uk}
To: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
Cc: "'Edmund Gierlik '" {gierlik-at-delta.sggw.waw.pl} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, March 13, 2001 4:07 AM


} I am getting confused about light sources and Ca ratio imaging. I
} have talked to people who use standard Hg arc sources and seem to
} have no problems. Others seem to feel that you need a stabilized
} power supply or a Xenon source for stability (see below). Are we
} talking about line voltage variations (which a line
} conditioner/voltage regulator ought to deal with) or actual arc
} source variation? Any opinions out there? Thanks- Dave



} From my observations of using both types of light source, the Xenon seems
} far more stable with time. You don't notice the fluctuations that you get
} with Hg bulbs. This is important for quantification, such as dual excitation
} ratio methods (eg. Fura-2).
}
} Stephen H. Cody,
} Colon Molecular and Cell Biology Laboratory,
} Ludwig Institute for Cancer Research,
} Post Office Royal Melbourne Hospital,
} Parkville, Victoria 3050, Australia.
}
} Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
} email: stephen.cody-at-ludwig.edu.au
} www.ludwig.edu.au/confocal
}
}
} } ----------
} } From: Tom Phillips[SMTP:PhillipsT-at-MISSOURI.EDU]
} } Reply To: Confocal Microscopy List
} } Sent: Wednesday, 28 February 2001 2:08
} } To: CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU
} } Subject: Re: Xenon lamps
} }
} } All true but I believe one disadvantage is the peak illumination is
} } less for some fluorochromes such as DAPI. We see a difference in our
} } two systems that have Hg or Xe.
} }
} }

--

************************************************************
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269-3125
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************


From daemon Tue Mar 13 09:13:43 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 13 Mar 2001 09:09:16 -0600
Subject: Re: Ca ratio imaging and arc source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Two standard points. These are not about power output but do
relate to the choice of arc type (archtype??) which seemed to be on
the questioner's mind. Forgive me if this is too obvious.

One is that the output of a xenon arc is much more uniform
across the spectrum compared to the steep peaks of the mercury arc.
For ratios with different excitation wavelengths, in principle
difference in intensity between the two wavelengths doesn't matter,
but in practice when the intensities differ alot, there can be
problems. Therefore, if you are going to be working with a variety of
wavelength pairs (lots of different dyes and applications) the
continuity of the xenon will probably be helpful; whereas, if you
will stick to a single pair of wavelengths, or ratio emitted light,
then even spectral quality might not matter.

The other issue is that xeon arcs tend to give off lots more
ozone than mercury arcs and so usually require significant venting.
This can be a nuisance depdending on the lay of the land, etc.

As ever,
Tobias Baskin

}
} } I am getting confused about light sources and Ca ratio imaging. I
} } have talked to people who use standard Hg arc sources and seem to
} } have no problems. Others seem to feel that you need a stabilized
} } power supply or a Xenon source for stability (see below). Are we
} } talking about line voltage variations (which a line
} } conditioner/voltage regulator ought to deal with) or actual arc
} } source variation? Any opinions out there? Thanks- Dave
}
}
}
} } From my observations of using both types of light source, the Xenon seems
} } far more stable with time. You don't notice the fluctuations that you get
} } with Hg bulbs. This is important for quantification, such as dual excitation
} } ratio methods (eg. Fura-2).
} }
} } Stephen H. Cody,
} } Colon Molecular and Cell Biology Laboratory,
} } Ludwig Institute for Cancer Research,
} } Post Office Royal Melbourne Hospital,
} } Parkville, Victoria 3050, Australia.
} }
} } Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
} } email: stephen.cody-at-ludwig.edu.au
} } www.ludwig.edu.au/confocal
} }
} } } ----------
} } } From: Tom Phillips[SMTP:PhillipsT-at-MISSOURI.EDU]
} } } Reply To: Confocal Microscopy List
} } } Sent: Wednesday, 28 February 2001 2:08
} } } To: CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU
} } } Subject: Re: Xenon lamps
} } }
} } } All true but I believe one disadvantage is the peak illumination is
} } } less for some fluorochromes such as DAPI. We see a difference in our
} } } two systems that have Hg or Xe.
} } }
} } }
}
} --
}
} ************************************************************
} Dr. David Knecht
} Department of Molecular and Cell Biology
} University of Connecticut
} 75 N. Eagleville Rd. U-125
} Storrs, CT 06269-3125
} Knecht-at-uconnvm.uconn.edu
} 860-486-2200 860-486-4331 (fax)
} home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
} ************************************************************

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Tue Mar 13 09:58:44 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 13 Mar 2001 10:46:01 -0500 (EST)
Subject: Re: Agarose enrobed cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It could be a problem with penetration through the agar if cells are fixed
in glutaraldehyde after the agar is added. Glut crosslinks the agar so
that other solutions, including osmium and washes, can't penetrate
properly. (I know this from experience.) We rinse the monolayer briefly
and gently with warmed buffer (pour on, swirl, pour off). The buffer
should be 300 mOsm (check with an osmometer) and pH 7.2-7.4 (check with a
pH meter). Pour on the glut in buffer and let sit about 3 min (no more
than 5); scrape, pellet cells in the glut and let sit for another 30 min.
The pellets should be small (2 mm or so). If pellets are large, you can
loosen them with the applicator stick so that the glut can get around to
the underneath side. Just try not to disturb the clump too much. Remove
glut with pipette. Scrape out pellet with flattened end of applicator
stick. Enrobe with agar and then wash and stain as you would a piece of
tissue. If there's a lot of excess agar, you can trim it away with a
razor blade. This keeps them together and makes life much easier.

Some folks suspend cells in warm agar or gelatin before fixation and spin
them down in the agar or gelatin and then fix. We have had variable
staining this way, particularly if there is a lot of excess agar or the
agar solidifies before the cells get to the bottom of the tube. If you
can get a tight pellet by centrifuging fast in a warmed centrifuge (or
jacketed tube) before the agar solidifies so that the layer of agar
around each cell is thin, it will work. Do cut away the excess agar at
the top of the tube, and make cell blocks small.

We centrifuge in a microfuge tube that looks like this:

| |
| |
| |
| |
| |
\ /
| |
| |
U

(Sorry about the graphics.) I think they come from PGC Scientific (800
424 3300). Sorry, I don't have the catelog number; our bag has been
emptied, and our catelog has been "borrowed." They are about 2 " long
and 3 mm in dia; the inside of the very tip is the diameter of a paper
clip. This info was provided a while back, and maybe you can find it in
the archives.

Centrifuge at right angles so that the pellet doesn't get stuck on the
slanted neck of the tube. Then cut off the very bottom (below the cells)
and then cut off the tube above the cells. Push out the cell "log" with
a straightened paper clip. Chop into smaller logs.

Good luck. Address below if further questions.




On 12 Mar 2001, Bruce Cutler wrote:

} Date: 12 Mar 2001 12:58:48 -0600
} From: Bruce Cutler {bcutler-at-eureka.idl.ukans.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Agarose enrobed cells
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A user has encountered an interesting problem . When cells are scraped and
} pelleted and embedded directly in epoxy they appear OK under the scope. When
} they are enrobed in agarose and then embedded the appearance becomes variable,
} although most cells appear very dense with contrast between organelles very low.
} This variability in appearance can be on the same grid square.
} Any ideas?
} Thanks
} Bruce
} Bruce Cutler
} Director, Microscopy & Electronic Imaging Laboratory
} University of Kansas
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Mar 13 10:32:10 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 13 Mar 2001 08:26:24 -0800
Subject: Re: TEM on cellulose and iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mary Gail,
Do you mean a layer of metallic iron? I think you should treat the cellulose
as wood or plant tissue and fix and embed, then cut a cross-section as you
would a plant stem. No staining necessary. You need a TEM or STEM with an
EDX on it and it will easily locate the iron to the ten nanometer scale.
At 03:05 PM 3/12/01 -0500, you wrote:
}
} Dear listers,
} I have a customer who wants TEM on a section of cellulose that is coated
} with a layer of Fe2. I have no idea about how to go about accomplishing
} this. He wants to see where the Fe2 is located in the cellulose matrix,
} and how deep it has penetrated. The sample is a layer of cellulose about
} 1/2 mm thick with a visible layer of Fe on the surface and it's in
} submerged in water.
} Any ideas out there?
} Thanks so much,
}
} Mary Gail Engle

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Mar 13 18:17:01 2001



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 13 Mar 2001 16:02:14 -0800
Subject: Amray service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I am manufacturing a numnber of items related to Amray service: extender
cards, etc.

Would any interested parties please contact me if you want any or have a
request for a service fixture.

I will be making these items available for "our cost". In other words, I
just want ot be re-imbursed for my expenses fro manufacturing these.

Regards,

Earl



From daemon Tue Mar 13 21:42:40 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Tue, 13 Mar 2001 19:37:27 -0800 (PST)
Subject: asbestos tests

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I was looking at some piping in our lab while sitting on the windowsill
and noticed a sign painted over. Looking closer it was a sign for
asbestos insulation that has been 80% painted over. I was told earlier
that there was no asbestos pipe insulation in the lab. I collected some
dust samples from near the fraying pipe insulation, on top of shelves,
floor, and the windowsill and imaged them in our SEM.

Can anyone with some experience with asbestos take a look at the images
at: http://wilfred.berkeley.edu/~gordon/webdust and see if any of the
images looks like a candidate for asbestos? I think only fd6, fd7, and
fd8 are asbestos, and the rest of the fibrous materials imaged are
cellulose binding agents used in the insulation.

I will examine the material by TEM and electron diffraction, but I was
wondering if someone could post the techniques they use for sampling from
the dust, and any other hints and techniques I should be aware of.

Thanks for your help,
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793





From daemon Tue Mar 13 22:59:48 2001



From: Markus F. Meyenhofer :      micro-at-mail.superlink.net
Date: Tue, 13 Mar 2001 22:58:07 -0600
Subject: Looking for parts for JEOL 100C URGENT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking for parts in the "left control panel housing B" for a JEOL 100C.
Please contact me off line.
Regards.
Markus F. Meyenhofer




From daemon Wed Mar 14 06:45:08 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Wed, 14 Mar 2001 10:51:56 +0100
Subject: Wyko Profilometer Data Formats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have some data from a Wyko optical profilometer which we would like to
work with offline & import into MATLAB for some further analysis. The lab
tells me that the Wyko data can be output in "OPD" format. Does anyone know
how we can covert this to a format we can work with.

Thanks

Tim

-------------------------------------------------------------------------
Tim E. Harper CEO CMP Cientifica
Tel. +34 91 640 7185
Fax. +34 91 640 7186



From daemon Wed Mar 14 07:50:19 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 3/13/01 7:37 PM
Subject: asbestos tests

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Gordon's comments concern me on several levels. The SEM, especially
without EDX, is not the best tool to identify asbestos fibers. The TEM and
diffraction can be absolutely definitive. However, I'm unaware of any EPA
or NIOSH protocol for taking dust to the TEM grid, though one could be
developed easily enough for Gordon's purposes. Gordon described friable
pipe insulation with an asbestos warning. Seems wiser to bring his
administration in on this situation. Does the UC system have some in-house
personnel that can run a PLM for him? The lack of such in house personnel
or an office dealing with asbestos abatement would be surprising for such a
large university. Even a commercial lab PLM is faily inexpensive. While
the amount of dust given off the pipe, barring some regular banging on the
pipe, is probably minimal, the wiser course of action is bringing in
someone who is well trained in dealing with asbestos pipe insulation. It
doesn't have to be removed, just managed.

Chuck Butterick
Engineered Carbons,Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,
I was looking at some piping in our lab while sitting on the windowsill
and noticed a sign painted over. Looking closer it was a sign for
asbestos insulation that has been 80% painted over. I was told earlier
that there was no asbestos pipe insulation in the lab. I collected some
dust samples from near the fraying pipe insulation, on top of shelves,
floor, and the windowsill and imaged them in our SEM.

Can anyone with some experience with asbestos take a look at the images
at: http://wilfred.berkeley.edu/~gordon/webdust and see if any of the
images looks like a candidate for asbestos? I think only fd6, fd7, and
fd8 are asbestos, and the rest of the fibrous materials imaged are
cellulose binding agents used in the insulation.

I will examine the material by TEM and electron diffraction, but I was
wondering if someone could post the techniques they use for sampling from
the dust, and any other hints and techniques I should be aware of.

Thanks for your help,
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron
MicroscopeLab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793








From daemon Wed Mar 14 08:35:56 2001



From: Kristi Snell :      snell-at-metabolix.com
Date: Wed, 14 Mar 2001 09:29:54 -0500
Subject: LM-thin sectioning and staining

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I am trying to develop a procedure to thin section plant leaves,
stain them with Nile blue, and visualize the stained leaves
by light microscopy.

I assume that a microtome would be the best way to perform
the thin sectioning but have I have no experience with
microtomes. Does anyone have suggestions on suitable
microtomes to purchase for such a procedure? If so,
where is the best place to purchase them?

Thank you.

-Kristi Snell

--
Dr. Kristi D. Snell
Metabolix, Inc.
303 Third St.
Cambridge, MA 02142
Fax: 617-492-1996
Phone: 617-492-0505 x 229




From daemon Wed Mar 14 08:44:43 2001



From: Brian Wajdyk :      Brian.Wajdyk-at-onsemi.com
Date: Wed, 14 Mar 2001 07:40:51 -0700
Subject: EDS detector chracterization: sensitivity/efficiency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists,

Fellow Microscopists,

I want to track the performance of our EDS detector over time. We
currently
check: peak to background ratios via peak-to-tail of Mn k-alpha,
symmetry
via Full Width Tenth Max, peak offset via centeroid of the Mn k-alpha
and
L-alpha to known values, and resolution via Full Width Half Max. What I
don't have is a good way to determine sensitivity of the detector to
determine when to remove ice/hydrocarbon build up. In the past I used a
80%Mn 20%Cu standard and looked at the ratio of Cu k-alpha to Mn l-alpha
because at 10kV accelerating voltage, the peaks are nearly a 1:1 ratio.
Unfortunately I no longer have this standard or the means to acquire a
new
one thanks to the slow down in the e semiconductor industry. My best
guess
would to be to clean the detector then use the k-alpha to l-alpha ratio
of
Cu. However I know that we have the best microscopists in the world at
our
disposal. Any suggestions for sensitivity tracking, or other useful
detector characterizations I may have missed, would be greatly
appreciated.

Sincerely,

Brian Wajdyk
Sr. Electron Microscopist
On Semiconductor
brian.wajdyk-at-onsemi.com
602-244-4883


From daemon Wed Mar 14 09:35:41 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 14 Mar 2001 10:17:38 -0600
Subject: RE: RE: ESEM-Need presentation of phase transition.

Contents Retrieved from Microscopy Listserver Archives
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Brian -

A few years back when I took an EDS course put on by NORAN, they said
keeping track of those K-alpha: L-alpha ratios should give you a running
record of your detector sensitivity. I've been doing that ever since, and
you can actually see those ratios change over time, as your detector window
gets dirty.
Another trick they mentioned (though I've never tried it myself) is to
run a spectrum on some ordinary Teflon tape (yes, the kind you use for
plumbing repairs). Teflon, apparently, contains no oxygen, so the presence
of O in the resulting spectrum can only be from ice buildup. Clever, no?
I also routinely do the Full Width Half Max test whenever I do a
calibration.
Good luck.

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia

----- Original Message -----
} From: "Brian Wajdyk" {Brian.Wajdyk-at-onsemi.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 14, 2001 10:40 AM


I do not recall any problems with salt/water imaging.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----------------------------------------------------------------------.
}
}
} We turned water to ice on plant surfaces using the Peltier
} stage. We did not take any nice pictures (I have a few
} ugly ones!). It was an idea re ice nucleation on plants
} for a student project idea that we did not follow up.
}
} Dave
}
Like Dave, I've sometimes taken pictures of ice/water etc in our ESEM, but
ice is such a good insulator, (I guess), the images never seem to come out
well. So, I've never saved any. Also, salt/water interactions can be
difficult too, since the salt really seems to want to charge a lot, to the
detriment of image acquisition. Yes, you can play around with KeV and all
that to minimize these effects, but it's still a very challenging "shoot" to
get useable images.

Frank Thomas
Geological Survey of Canada (Atlantic)
Dartmouth, Nova Scotia



From daemon Wed Mar 14 10:35:34 2001



From: Martin J. Roe :      m.roe-at-mluri.sari.ac.uk
Date: Wed, 14 Mar 2001 16:30:56 +0000
Subject: Re: EDS detector chracterization: sensitivity/efficiency

Contents Retrieved from Microscopy Listserver Archives
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Brian,
We work in a UKAS accredited laboratory and have a Pentafet light
element detector with rotating turret device for windowless and thin
window analysis. I perform a test with a Ni sample every month
using the detector in its windowless mode. We monitor the ratio of
the counts in the Ni L and Ni K peaks (NiL/NiL+NiK) (knowing what
the value should be immediately after reconditioning). When the
monthly value falls below a certain predetermined value, we simply
perform the reconditioning routine.
I suppose if you don't have a light element detector then the Cu
standard you suggest is much better for a decent sized L line.
I find that the above works very well for tracking the detector's
sensitivity over a period of time.
Best of luck
Martin Roe

What I don't have is a good way to determine
sensitivity of the detector to determine when to remove
ice/hydrocarbon build up. In the past I used a 80%Mn 20%Cu
standard
and looked at the ratio of Cu k-alpha to Mn l-alpha because at 10kV
accelerating voltage, the peaks are nearly a 1:1 ratio. Unfortunately
I no longer have this standard or the means to acquire a new one
thanks to the slow down in the e semiconductor industry. My best
guess would to be to clean the detector then use the k-alpha to
l-alpha ratio of Cu. However I know that we have the best
microscopists in the world at our disposal. Any suggestions for
sensitivity tracking, or other useful detector characterizations I may
have missed, would be greatly appreciated.

Sincerely,

Brian Wajdyk
Sr. Electron Microscopist
On Semiconductor
brian.wajdyk-at-onsemi.com
602-244-4883


Martin J. Roe
Macaulay Land Use Research Institute
Craigiebuckler
Aberdeen
Scotland
UK
Phone 01224 318611
e-mail m.roe-at-mluri.sari.ac.uk


From daemon Wed Mar 14 10:52:16 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Wed, 14 Mar 2001 11:50:46 -0500
Subject: RE: asbestos tests

Contents Retrieved from Microscopy Listserver Archives
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I'd say some of those fibers are definitely "candidates". They are also large
enough that you could analyze by PLM. If you want to do the TEM for fun or for
the experience: dust samples are usually suspended in water and filtered onto a
PC or MCE filter. I'm sure someone will post the appropriate reference, I
can't seem to find it here.

Matt

On Tuesday, March 13, 2001 10:37 PM, Gordon Vrololjak
[SMTP:gvrdolja-at-nature.Berkeley.EDU] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I was looking at some piping in our lab while sitting on the windowsill
} and noticed a sign painted over. Looking closer it was a sign for
} asbestos insulation that has been 80% painted over. I was told earlier
} that there was no asbestos pipe insulation in the lab. I collected some
} dust samples from near the fraying pipe insulation, on top of shelves,
} floor, and the windowsill and imaged them in our SEM.
}
} Can anyone with some experience with asbestos take a look at the images
} at: http://wilfred.berkeley.edu/~gordon/webdust and see if any of the
} images looks like a candidate for asbestos? I think only fd6, fd7, and
} fd8 are asbestos, and the rest of the fibrous materials imaged are
} cellulose binding agents used in the insulation.
}
} I will examine the material by TEM and electron diffraction, but I was
} wondering if someone could post the techniques they use for sampling from
} the dust, and any other hints and techniques I should be aware of.
}
} Thanks for your help,
} Gordon.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}



From daemon Wed Mar 14 11:12:35 2001



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Wed, 14 Mar 2001 12:14:24 -0500
Subject: SUMMARY -- laser vision correction for microscopists? (long)

Contents Retrieved from Microscopy Listserver Archives
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Many thanks to all those who answered my query on
laser vision correction. I received 17 responses:

9 were considering the procedure and expressed
interest in seeing the responses, hence this
summary.

1 provided the following informational site:

http://www.manufacturing.net/magazine/dn/archives/2001/dn0226.01/feature1.html

This is a general site for Design News magazine; search
that site for the term "lasik" to find an uncritical article
featuring the inventor of the laser-vision machine now
marketed by Visx. Even though it's a puff piece, it contains
a great deal of info on the process.

2 recommended against the surgery, citing the risk of
bad results for small gain if you have good correction
now with glasses or contacts. Here are sites they cited
with good info and extensive warnings of potential problems,
and some additional sites I found in my own searches:

http://www.americaneye.com/bbs3/

This is a bulletin board where you can read many
personal comments.

http://www.surgicaleyes.com/

This is a site founded by people who had unsuccessful
laser vision correction resulting in complications. Its
major point is that success defined by Snellen eye
chart acuity may be inadequate.

http://wakeup.to/lasik

The site's name should tell you its slant on the topic.

http://www-psy.ucsd.edu/~mm/eyeknowwhy/index.htm

Very well-organized site, balanced viewpoint, but
a bit out of date (last updated 1999). Well worth
a careful reading anyway.

http://www.vision-institute.com/director/article-prkvlasik.html

Detailed study results comparing PRK and LASIK
outcomes. 3 years old (1998, therefore procedures
done at least a year earlier). 220 eyes in the study.
Interesting point (for me): 11.8% of PRK eyes and
3.2% of LASIK eyes reported a loss of 2 Snellen
lines or more of best spectacle-corrected visual
acuity (in English, you can't see as well as before
even with glasses).

http://www.lasikwithprobst.com/lasik/fdastudy/default.htm

A more recent study (1999) with better outcomes.
Equipment and experience makes a significant
difference; be aware the technology changes
fairly rapidly. 2-line loss rates from 2% to 0.6%
in larger groups (1000+ and 700+ eyes).

http://www.allaboutvision.com/visionsurgery/outcomes.htm

More outcomes data; general, but recent.

http://www.ohioeye.com/refractive.html

A very good Feb. 2000 presentation of FDA pre-market
approval data for several laser systems. 2-line
loss is down to 0.3% with one system, but they
show glare and night-vision problems reported
by ~10% of patients. Remarkable Snellen acuity
results: 87% 20/20 post-op without lenses.

5 respondents to my query had actually had laser surgery.
All ultimately reported being satisfied with the results. 4
had no side effects at all. 3 reported good to excellent
results immediately with no side effects. One (57 years
old, farsighted) reported better post-op vision than previous
lens-corrected vision. 1 respondent cited trouble for
almost 1 year after surgery (couldn't see TEM images
properly, trouble adjusting astigmatism), and said he
was "initially devastated". However, he said he
adjusted and/or the problems corrected themselves after
a year. He can now handle all imaging tasks, and was
happier overall than with glasses.

My personal decision has been to wait and see. I'm still not
satisfied with the fairly high reported incidence of glare and
night-vision problems, which I think are related to limited
correction diameters compared to the dilated pupil of a
larger-than-average eye (mine are). The changes I saw
over just a few years of clinical trial results convinced me
this is still evolving technology. The risks, while small,
are still too high for me to accept in a purely elective
procedure.

Thank you for your patience with this long-winded
summary. Hope the info is useful to some of you.

Rick Mott (still reaching for his specs in the morning)




From daemon Wed Mar 14 11:12:35 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 14 Mar 2001 09:08:13 -0800
Subject: Re: asbestos tests

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gordon,
As someone in an old building with asbestos floor tiling, asbestos fume
cupboard lining and old technicians who like to machine asbestos, I have
looked at lots of samples of asbestos insulation. First, when asbestos is
used, it is usally 30 % of the materials by volume, so it is very evident.
Second it is characterized by very long, fairly straight fibres that divide
down to smaller and smaller fibres, through and beyond the range of the SEM
magnification. By that critierion, none of your pictures look like they
contain asbestos. It helps to have looked at a sample so you can recognise
it. Third, I check all suspicious fibres by EDX. The crysotile asbestos used
in most insulation has a distictive Mg-Si spectrum, with a little Ca and Fe,
that isn't found in most minerals. If it has that signature, then I would
suspect asbestos and do the TEM. At 07:37 PM 3/13/01 -0800, you wrote:
} Hello,
} I was looking at some piping in our lab while sitting on the windowsill
} and noticed a sign painted over. Looking closer it was a sign for
} asbestos insulation that has been 80% painted over. I was told earlier
} that there was no asbestos pipe insulation in the lab. I collected some
} dust samples from near the fraying pipe insulation, on top of shelves,
} floor, and the windowsill and imaged them in our SEM.
}
} Can anyone with some experience with asbestos take a look at the images
} at: http://wilfred.berkeley.edu/~gordon/webdust and see if any of the
} images looks like a candidate for asbestos? I think only fd6, fd7, and
} fd8 are asbestos, and the rest of the fibrous materials imaged are
} cellulose binding agents used in the insulation.
}
} I will examine the material by TEM and electron diffraction, but I was
} wondering if someone could post the techniques they use for sampling from
} the dust, and any other hints and techniques I should be aware of.
}
} Thanks for your help,
} Gordon.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Mar 14 11:30:33 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Wed, 14 Mar 2001 17:26:02 +0000 (GMT)
Subject: FIB machine - would like info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,
I have the opportunity to obtain a Seiko SMI8300 focused ion beam machine. I can't find out very much about it (I didn't even know Seiko made them - I suspect they are usually native to Japan). Does anyone have any useful information or experience? Could I use one to make TEM specimens?

Many thanks,

Richard


==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Wed Mar 14 11:52:22 2001



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Wed, 14 Mar 2001 12:33:44 -0500
Subject: Brief PS: laser vision correction for microscopists?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One last note: of the 4 respondents who enthusiastically
recommended the procedure, all were in agreement on the
importance of finding a highly experienced surgeon.
Complication rates are inversely related to experience.

Thanks,

Rick Mott




From daemon Wed Mar 14 12:26:54 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Wed, 14 Mar 2001 10:22:55 -0800
Subject: RE: EDS detector chracterization: sensitivity/efficiency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank,

I've never tried this either, but doubt it should work for the stated reason. A
layer of ice on the detector (actually the oxygen in the ice) will act mostly as
an x-ray absorber rather than a source of O x-rays. In fact, the ice will be a
poor absorber for O-K x-rays because the oxygen x-ray energy is below its
absorption edge.

Larry Thomas
Pacific Northwest National Laboratory
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto:Larry.Thomas-at-pnl.gov




----------
From: Frank Thomas
Sent: Wednesday, March 14, 2001 7:30 AM
To: Brian Wajdyk; Microscopy-at-sparc5.microscopy.com
Subject: Re: EDS detector chracterization: sensitivity/efficiency

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Brian -

A few years back when I took an EDS course put on by NORAN, they
said
keeping track of those K-alpha: L-alpha ratios should give you a running
record of your detector sensitivity. I've been doing that ever since,
and
you can actually see those ratios change over time, as your detector
window
gets dirty.
Another trick they mentioned (though I've never tried it myself) is
to
run a spectrum on some ordinary Teflon tape (yes, the kind you use for
plumbing repairs). Teflon, apparently, contains no oxygen, so the
presence
of O in the resulting spectrum can only be from ice buildup. Clever, no?
I also routinely do the Full Width Half Max test whenever I do a
calibration.
Good luck.

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia

----- Original Message -----
} From: "Brian Wajdyk" {Brian.Wajdyk-at-onsemi.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 14, 2001 10:40 AM
Subject: EDS detector chracterization: sensitivity/efficiency


}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Fellow Microscopists,
}
} Fellow Microscopists,
}
} I want to track the performance of our EDS detector over time. We
} currently
} check: peak to background ratios via peak-to-tail of Mn k-alpha,
} symmetry
} via Full Width Tenth Max, peak offset via centeroid of the Mn k-alpha
} and
} L-alpha to known values, and resolution via Full Width Half Max. What
I
} don't have is a good way to determine sensitivity of the detector to
} determine when to remove ice/hydrocarbon build up. In the past I used
a
} 80%Mn 20%Cu standard and looked at the ratio of Cu k-alpha to Mn
l-alpha
} because at 10kV accelerating voltage, the peaks are nearly a 1:1
ratio.
} Unfortunately I no longer have this standard or the means to acquire a
} new
} one thanks to the slow down in the e semiconductor industry. My best
} guess
} would to be to clean the detector then use the k-alpha to l-alpha
ratio
} of
} Cu. However I know that we have the best microscopists in the world
at
} our
} disposal. Any suggestions for sensitivity tracking, or other useful
} detector characterizations I may have missed, would be greatly
} appreciated.
}
} Sincerely,
}
} Brian Wajdyk
} Sr. Electron Microscopist
} On Semiconductor
} brian.wajdyk-at-onsemi.com
} 602-244-4883
}
}





From daemon Wed Mar 14 13:32:13 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 14 Mar 2001 13:26:40 -0600
Subject: Computer: JPEG binary problems on server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps a Macintosh, computer guru could figure this one out.

We are capturing JPEG images from a standard (Sony, analog 470 line,
NTSC) video camera using a program called Reel Eyes. The images are
captured on a Mac 8500 via the built-in S-Video port and saved as
Quicktime JPG's (as mandated by the Reel Eyes program). When we put
these images on our server they now appear with ".bin" appended to
the file name so that the new image is described as a
"Application/x-macbinary". Mac folks can open the files after
processing through Stuffit but PC folks are unable to deal with the
files. Anyway, this is an additional step that should not be present.

We can remove this problem by re-opening each of the images in
Photoshop and saving them without the Thumbnail (or preview). So, I
conclude that Quicktime is always saving with the Thumbnails in
place. Does anyone know of a way that we can prevent the thumbnails
from occurring in Quicktime?

Any other suggestions would be most welcome.

Many thanks,

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Mar 14 15:54:24 2001



From: Gordon Nord :      gnord-at-mindspring.com
Date: Wed, 14 Mar 2001 16:51:20 -0500
Subject: Re: Computer: JPEG binary problems on server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

The problem is that your files are encoded as Macbinary (.bin) before
being stuffed. Windows machines can't decode .bin files unless they have the
windows version of Stuffit expander. Encourage them to get it.

To correct this you need to change a preference setting.

In Magic Menu or Dropstuff preferences, go to the "Cross-Platform" or
"MacBinary" icon and choose "Never" (instead of "Smart"). There is no
way to change this preference in the StuffIt Deluxe application.

The URL for this information is
{http://www.aladdinsys.com/support/techsupport/mac/dlx/dlx611.html}

Happy capturing.
Gordon Nord
"My real job is Mineralogy"

"John J. Bozzola" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Perhaps a Macintosh, computer guru could figure this one out.
}
} We are capturing JPEG images from a standard (Sony, analog 470 line,
} NTSC) video camera using a program called Reel Eyes. The images are
} captured on a Mac 8500 via the built-in S-Video port and saved as
} Quicktime JPG's (as mandated by the Reel Eyes program). When we put
} these images on our server they now appear with ".bin" appended to
} the file name so that the new image is described as a
} "Application/x-macbinary". Mac folks can open the files after
} processing through Stuffit but PC folks are unable to deal with the
} files. Anyway, this is an additional step that should not be present.
}
} We can remove this problem by re-opening each of the images in
} Photoshop and saving them without the Thumbnail (or preview). So, I
} conclude that Quicktime is always saving with the Thumbnails in
} place. Does anyone know of a way that we can prevent the thumbnails
} from occurring in Quicktime?
}
} Any other suggestions would be most welcome.
}
} Many thanks,
}
} John B.
}
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ##############################################################

--
Gordon Nord
Small Business Network Design and Construction
Macintosh and Windows - Solutions and Conflicts

Nord Consultants
20594 Cornstalk Terrace
Ashburn VA 20147

Voice 703-723-2798 (Home Office)
Cell 703-403-2776 (Mobile Office)
Email gnord-at-mindspring.com


From daemon Wed Mar 14 16:11:25 2001



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Wed, 14 Mar 2001 15:07:17 -0700
Subject: Preparing cross-section of coated fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Werner,

Yes, I did use Hitach 4500S and 3500N to study some coated fibers in the
recent past years. The coating layers were up to three.

General speaking, I think this kind of preparation needs patient and do it
properly at every step as the processing goes.

The steps I did for preparing the coated fibers for SEM are
1. Cut the fibers carefully into about 1 cm length of pieces
2. Arrange them in a longitudinal direction (do not hurt them)
3. Hold one end of the fibers by using a pair of tweezers
4. Apply thin M-Bond (16) on to the fibers
Since the M-bond is so thin, it will go along with the fibers and
surround almost every fiber very well.
5. Leave the fibers in the air (room temperature) overnight. Do not let the
wet part touch any solid surface such as metal or microscope slide.
Otherwise when it cures, it is very difficult to separate them. Another way
to cure it is putting the fiber with the tweezers on to a hot plate at 130
C for one hour. Do not let the wet part touch the hot plate.
6. When the fibers are cured, embed them into epoxy to make a big enough
piece so that you can hold and polish it for SEM.
At this step, you have to avoid trapping air in the epoxy. Especially
when a fabric of fibers is going to be embedded into epoxy, you have to use
a pump to get rid of air bobbles before curing.
You may choose a kind of epoxy which can be cured in couple of hours in
air (room temperature) or on a hot plate for about 1 to 2 hours at 130 C.
The epoxy I used is a kind of mixture of two parts.
7. Then you may follow the routing procedures for polishing SEM samples,
i.e., grinding first then polishing, from coarse to fine carefully.

Finally if you are going to study a fabric, then you can cut a size of 1 x
1 cm2, and hold it with a tweezers at a corner, and apply a layer of M-bond
(16) on both sides, put it onto a hot plate for curing. Then embed this
piece of fabric into epoxy. Again, you have to pump the air out before
curing.

Good luck.

Zhenquan Liu







} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
Center for Solid State Science
Room PSA213
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------




From daemon Wed Mar 14 16:13:40 2001



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Wed, 14 Mar 2001 15:10:12 -0700
Subject: Looking for Dr. Zhiping Wang

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Zhiping Wang,

I am sure you are checking with this listserver very often. Please tell me
your telephone number and address.

Cheers!

Zhenquan Liu (Dr.)

------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
Center for Solid State Science
Room PSA213
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------




From daemon Wed Mar 14 16:48:32 2001



From: Pradyumna Prabhumirashi :      p-prabhumirashi-at-northwestern.edu
Date: Wed, 14 Mar 2001 16:47:13 -0600
Subject: Problems with Gatan IBT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We have a Gatan Duomill IBT Model 600. We have been having some problems
with it lately.
When I turn on the IBT, the vacuum in the main chamber works fine. When
pumping
down the specimen chambers, the left side does not pump at all. The right
side pumps to the proper level, but when you lower the sample into the
chamber, it takes a while for the vacuum to recover. There is no vacuum
instability during sample rotation. There is no arcing in the guns. The
argon flow is at the proper level.
We have done the following:
-cleaned, regreased and changed o-rings in the Whisperlock Assembly,
sample viewing port, sample chamber, vent/vac buttons, and ion guns
-changed the cathode tubes
-dusted out the main sample chamber
-check the vacuum pumps and topped off oil in the roughing pump
-DP seems to be running fine

Still it doesn't seem to help. We are lost. Any suggestions about what might
be going wrong here? Thanks.
Prad

Pradyumna Prabhumirashi
Department of Materials Science
Northwestern University
Phone: (847)-491-7798
Fax: (847)-491-7820
http://vpd.ms.northwestern.edu/prad.htm






From daemon Wed Mar 14 16:55:25 2001



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Wed, 14 Mar 2001 16:51:06 -0600
Subject: EDS detector chracterization: sensitivity/efficiency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have used the k:alpha:l-alpha ratio for copper and nickel for many years
as a measure of ice buildup on our detectors. Either ratio is very sensitive
to ice buildup. With single element standards you don't have to worry if the
mixture of two elements in your standard has changed. For sources we use
copper or nickel TEM grids which are cheap and available from all the EM
vendors.

Regards,
Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com



Brian.Wajdyk-at-onsemi.com on 03/14/2001 10:24:35 AM
To: Microscopy-at-sparc5.Microscopy.Com
cc:


Fellow Microscopists,

Fellow Microscopists,

I want to track the performance of our EDS detector over time. We
currently
check: peak to background ratios via peak-to-tail of Mn k-alpha,
symmetry
via Full Width Tenth Max, peak offset via centeroid of the Mn k-alpha
and
L-alpha to known values, and resolution via Full Width Half Max. What I
don't have is a good way to determine sensitivity of the detector to
determine when to remove ice/hydrocarbon build up. In the past I used a
80%Mn 20%Cu standard and looked at the ratio of Cu k-alpha to Mn l-alpha
because at 10kV accelerating voltage, the peaks are nearly a 1:1 ratio.
Unfortunately I no longer have this standard or the means to acquire a
new
one thanks to the slow down in the e semiconductor industry. My best
guess
would to be to clean the detector then use the k-alpha to l-alpha ratio
of
Cu. However I know that we have the best microscopists in the world at
our
disposal. Any suggestions for sensitivity tracking, or other useful
detector characterizations I may have missed, would be greatly
appreciated.

Sincerely,

Brian Wajdyk
Sr. Electron Microscopist
On Semiconductor
brian.wajdyk-at-onsemi.com
602-244-4883








From daemon Wed Mar 14 17:38:41 2001



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 14 Mar 2001 15:35:16 -0800
Subject: Reichert Frigo-cut repairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just got a call from a researcher on campus looking for service on a
Reichert Frigo-Cut type 2800, s/n 0110117 microtome. I am not familiar
with this piece of equipment (we don't do light level microtomy) and I do
not know this gentleman but he had already contacted one northern
California service and was quoted $130/hr p to p. He balked. I explained
that I had been quoted as much from services in New Hampshire. If anyone
has a resource for repairing one of these microtomes in the Northern
California region I will pass the info back to this person.

Thanks in advance.


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Wed Mar 14 18:19:11 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 14 Mar 2001 16:14:47 -0800 (PST)
Subject: Re: asbestos tests

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all of your replies,
We had someone from our physical plant come in, and they revealed that the
colour of the painted over sign indicates asbestos - free - insulation.
Wierd scare for me since the painter covered the most important part of
the label - that the insulation was asbestos free, not asbestos. They'll
come back to tape back up all of the loose insulation tomorrow.

Since one of the specimens I looked at is very similar in morphology to
asbestos, they'll be doing some wipe tests and counts by polarized light
microscopy for asbestos particles to be sure. It likely was left behind
from when the original asbestos insulation was removed about 10 years ago
or so.

I appreciate all of the replies on the thread and supportive messages.
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Wed, 14 Mar 2001, Mary Mager wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Gordon,
} As someone in an old building with asbestos floor tiling, asbestos fume
} cupboard lining and old technicians who like to machine asbestos, I have
} looked at lots of samples of asbestos insulation. First, when asbestos is
} used, it is usally 30 % of the materials by volume, so it is very evident.
} Second it is characterized by very long, fairly straight fibres that divide
} down to smaller and smaller fibres, through and beyond the range of the SEM
} magnification. By that critierion, none of your pictures look like they
} contain asbestos. It helps to have looked at a sample so you can recognise
} it. Third, I check all suspicious fibres by EDX. The crysotile asbestos used
} in most insulation has a distictive Mg-Si spectrum, with a little Ca and Fe,
} that isn't found in most minerals. If it has that signature, then I would
} suspect asbestos and do the TEM. At 07:37 PM 3/13/01 -0800, you wrote:
} } Hello,
} } I was looking at some piping in our lab while sitting on the windowsill
} } and noticed a sign painted over. Looking closer it was a sign for
} } asbestos insulation that has been 80% painted over. I was told earlier
} } that there was no asbestos pipe insulation in the lab. I collected some
} } dust samples from near the fraying pipe insulation, on top of shelves,
} } floor, and the windowsill and imaged them in our SEM.
} }
} } Can anyone with some experience with asbestos take a look at the images
} } at: http://wilfred.berkeley.edu/~gordon/webdust and see if any of the
} } images looks like a candidate for asbestos? I think only fd6, fd7, and
} } fd8 are asbestos, and the rest of the fibrous materials imaged are
} } cellulose binding agents used in the insulation.
} }
} } I will examine the material by TEM and electron diffraction, but I was
} } wondering if someone could post the techniques they use for sampling from
} } the dust, and any other hints and techniques I should be aware of.
} }
} } Thanks for your help,
} } Gordon.
} Regards,
} Mary
}
} Mary Mager
} Electron Microscopist
} Metals and Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} tel: 604-822-5648
} e-mail: mager-at-interchg.ubc.ca
}
}




From daemon Thu Mar 15 02:33:28 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 15 Mar 2001 09:26:11 +0100
Subject: re : EDS sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian

A few years ago, a EDS reparer explain me a test, which seems to be
interresting, to check the "health" of a detector with UTW, but I never
have tried it.

With a fluorine (CaF2) sample at 10 keV, You should have a ratio of one
betwween F K alpha and Ca K alpha. When not, or when it diminish in the
time (less F), you have ice (absorbtion effect of F by O2). (Why 10 kev?
Is 10 keV not a bit high for F. It think it's a compromise between the
window caracteristics and the primary energy).

Same thing with quartz (SiO2), at 5 keV, and than, if O diminish, you have
oil on the window (absorbtion effect of O2 by C).

As I said, I never tried this test, the CaF2 and SiO2 samples are in my
drawer, waiting for polishing. I suppose that geologist should have an
advice about that, but of course they don't often work at 5 keV for EDS.

I usually use a aluminum sample holder, with a piece of carbon adhesive
tape and a copper foil, and I take a spectrum with a quarter of the SEM
screen on the copper, a quarter on the carbon, and the half on the
aluminium,at low mag, and at 5 keV. I adust the probe current to a preset
value (typically a few nA, and each time the same value) mesured on the
carbon tape (less SE and BSE, better would be a Faraday cup). So I see if
the sensitivity on C K, Cu L and Al K seems to diminish from on mesure to
an other. In such condition you'll see that the count rate (and of course,
the spectrum shape) is very sensitive to window or detector contamination
(with allways the same probe current, time constant, etc.). You can
imagine similar test with for exemple, Vandium L and silicon K, etc.

best regard

Jacques


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Mar 15 02:53:32 2001



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Thu, 15 Mar 2001 08:49:59 -0000
Subject: IEM: Double labelling of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Microscopists,

I was asked to perform double labelling of surface antigens on
leukaemia cell cultures and cord blood progenitors.

In the past I used a pre-embedding protocol for single labelling
which worked quite well.

I would like to continue with a pre-embedded protocol, but both
primary antibodies are raised in the same animal and I wonder how
I can block unspecific labelling in that case.

(I have a protocol for post-embedded double staining with primary
AB raised in the same animal, but would prefer to use that as a
last resort)

So far I used a 10 nm gold F(ab)2- AB - if I use a 5nm gold F(ab)2
AB will I be able to distinguish them in the TEM?

I would appreciate your input very much.

Many thanks

Claudia


Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk


From daemon Thu Mar 15 04:01:25 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 15 Mar 2001 04:56:05 -0500
Subject: Preparing cross-section of coated fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I have a technique that I use with clients on a wide range of fibres which
takes very little time.

1. Drill two or three holes through two SEM stubs placed face to face.
2. Pass the fibres through the holes.
3. Fix in place with a water based carbon solution, make sure the
fibre/stub interface is well wetted.
4. When fully dry place in liquid nitrogen CARE!!.
5. When the bubbling stops lift out CARE!!
6. Place on an insulating surface and with a knife strike the
interface between the two stubs - the system will fracture.
7. When dried off you have two sets of surfaces to look at in LM or
SEM.

It works great on a number of differing media other than fibres, the only
snag is they must not be affected by the water base.

Steve Chapman
Senior Consultant Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Thu Mar 15 07:15:52 2001



From: Marco Arienti :      marienti-at-tiscalinet.it
Date: Thu, 15 Mar 2001 14:08:46 +0100
Subject: TEM maintenance CM10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have no big experience with Philips microscopes, but I need some
information.

A CM10 installed about 10 years ago have some vacuum problems.

Looks like the Ion Getter Pump is not working anymore.

As far as I understood the problem may be that there is no more Titanium in
the pump.

Is possible In this one to change the only grids (like in the Leibold pumps)
or the all pump have to be exchanged?

It is a pump manufactured from Edwards, any info about the model?

Thanks a lot.



Marco Arienti

www.electronrescue.com






From daemon Thu Mar 15 07:59:58 2001



From: John Bonevich :      john.bonevich-at-nist.gov
Date: Thu, 15 Mar 2001 08:55:45 -0500
Subject: Re: Computer: JPEG binary problems on server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I imagine that the problem arises when you transfer the files to the
server. I'll assume that you are using some FTP program such as Fetch. In
the upload preferences, make sure that you are transferring non-text data
in the "raw data" format, as opposed to MacBinary, etc. Also, make sure
that the FTP doesn't append a ".bin" to raw data.

Remember you still need to have a ".jpg" at the end of the filename or else
the Windows boxes still won't know how to handle the file (sigh...). There
are numerous utilties on the Mac to batch process the renaming of files for
cross-platform compatibility.

Hope this helps,
John B.

}
} Perhaps a Macintosh, computer guru could figure this one out.
}
} We are capturing JPEG images from a standard (Sony, analog 470 line,
} NTSC) video camera using a program called Reel Eyes. The images are
} captured on a Mac 8500 via the built-in S-Video port and saved as
} Quicktime JPG's (as mandated by the Reel Eyes program). When we put
} these images on our server they now appear with ".bin" appended to
} the file name so that the new image is described as a
} "Application/x-macbinary". Mac folks can open the files after
} processing through Stuffit but PC folks are unable to deal with the
} files. Anyway, this is an additional step that should not be present.
}
} We can remove this problem by re-opening each of the images in
} Photoshop and saving them without the Thumbnail (or preview). So, I
} conclude that Quicktime is always saving with the Thumbnails in
} place. Does anyone know of a way that we can prevent the thumbnails
} from occurring in Quicktime?
}
} Any other suggestions would be most welcome.
}
} Many thanks,
}
} John B.
}
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ##############################################################



From daemon Thu Mar 15 08:18:34 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 15 Mar 2001 08:13:36 -0600
Subject: Re: Computer: JPEG binary problems on server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:26 PM 3/14/01 -0600, John J. Bozzola wrote:
} The images are captured on a Mac 8500 via the built-in S-Video port and saved as Quicktime JPG's (as mandated by the Reel Eyes program). When we put these images on our server they now appear with ".bin" appended to the file name so that the new image is described as a "Application/x-macbinary". Mac folks can open the files after processing through Stuffit but PC folks are unable to deal with the files. Anyway, this is an additional step that should not be present.
} We can remove this problem by re-opening each of the images in Photoshop and saving them without the Thumbnail (or preview). So, I conclude that Quicktime is always saving with the Thumbnails in place. Does anyone know of a way that we can prevent the thumbnails from occurring in Quicktime?

The Mac's file system is slightly different than that on a PC or a Unix server.
Mac files have two parts, known as forks. The icon or thumbnail image resides
in the "resource fork". The regular JPEG image data that a PC expects is in
the "data fork." The resource fork also holds the Mac's association
between the file and the program that made it, while the PC uses the
file extension for that purpose.

MacBinary files are a way of wrapping up the data and resource forks into
a single file, such as for transmission via an e-mail attachment, ftp site,
etc. or in your case, handy for putting on a server that doesn't speak
with forked tongue.

You didn't say much about your server. If it's a WinNT server, your
admin can install some AppleTalk extensions that can make PC {-} Mac file
exchange a bit easier. They automatically manage the resource fork,
hiding it for PC programs but allowing networked Macs to store
and access it.

You also didn't say much about which PC programs you were using.
I don't know of any that handle MacBinary files. (I've written a
few that did, once upon a time, but they're obscure.) You may be
forced to either strip the files on the Mac, or use a utility
on the PC side to convert the MacBinary files to data-only files.

I don't think Quicktime thumbnails are the problem, I think it's
a MacBinary problem.

- John



From daemon Thu Mar 15 09:39:15 2001



From: pjcb-at-hei.co.kr
Date: Thu, 15 Mar 2001 09:33:48 -0600
Subject: Ask-A-Microscopist: analyzing stress around STI (shallow trench

Contents Retrieved from Microscopy Listserver Archives
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Email: pjcb-at-hei.co.kr
Name: Ju-chul Park

Organization: Hyundai Electronics

Education: Graduate College

Location: CheongJu Korea

Question: I have been analyzing stress around STI (shallow trench
isolation) in DRAM. In general, stress is
higher at bottom corner and top corner of STI, which is probed by my data.
But, the strange thing is that HOLZ line split and blurring occurs in CBED
pattern at the bottom of FOX (field oxide). I know that the split and
blurring is the sign of high stress. And I don't think that stress is
higher at the bottom of FOX.
So, my question is what makes line split and blurring except stress. Have
you seen that kind of phenomenon? Thank you for your answer.

---------------------------------------------------------------------------




From daemon Thu Mar 15 11:02:31 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Thu, 15 Mar 2001 09:47:36 -0800
Subject: Re: Preparing cross-section of coated fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Werner:

If you go to our new website at www.southbaytech.com, you will find a listing of
technical papers that you can request. There are several there dealing with coated
fibers. Please take a look. If any look interesting, submit a request and we will
send them out to you at no charge.

When you get to the site, click on Applications support then technical papers.
There are a lot of papers there, so the easiest way to find the ones you are
interested in is to use the "find on page" function typically found under your edit
command. If you type in "fiber", you'll find some relevant papers.

I hope this helps.

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Thu Mar 15 11:13:45 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 15 Mar 2001 17:19:39 +0000 (GMT Standard Time)
Subject: Re: TEM maintenance CM10

Contents Retrieved from Microscopy Listserver Archives
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Hi Marco,

The ion getter pump on the CM10 (12 and 20) does have a
finite life and if your machine is 10 years old with the
original IGP then I suspect that the pump could be
finished. I assume that it will not pump down properly on
the IGP but has a good penning gauge (P3) pressure.

The pump is not a standard Edwards pump as it has an extra
port so that it can pump both the column and the gun. As I
understand it the pump body is cut open to replace the
plates and then rewelded before being leak tested and baked.

There are companies that will do this for you but you are
without the pump while they do it, this may take two weeks
or more. FEI should hold replacement pumps that they would
swop in a day, more expensive but faster and more
convenient. It depends on your budget and facilities.

Good luck,
Ron



On Thu, 15 Mar 2001 14:08:46 +0100 Marco Arienti
{marienti-at-tiscalinet.it} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have no big experience with Philips microscopes, but I need some
} information.
}
} A CM10 installed about 10 years ago have some vacuum problems.
}
} Looks like the Ion Getter Pump is not working anymore.
}
} As far as I understood the problem may be that there is no more Titanium in
} the pump.
}
} Is possible In this one to change the only grids (like in the Leibold pumps)
} or the all pump have to be exchanged?
}
} It is a pump manufactured from Edwards, any info about the model?
}
} Thanks a lot.
}
}
}
} Marco Arienti
}
} www.electronrescue.com
}
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Mar 15 11:19:40 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 15 Mar 2001 17:16:13 +0000 (GMT Standard Time)
Subject: Re: Preparing cross-section of coated fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could you recommend a water based carbon solution?

Dave

On Thu, 15 Mar 2001 04:56:05 -0500 Steve Chapman
{PROTRAIN-at-CompuServe.COM} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} I have a technique that I use with clients on a wide range of fibres which
} takes very little time.
}
} 1. Drill two or three holes through two SEM stubs placed face to face.
} 2. Pass the fibres through the holes.
} 3. Fix in place with a water based carbon solution, make sure the
} fibre/stub interface is well wetted.
} 4. When fully dry place in liquid nitrogen CARE!!.
} 5. When the bubbling stops lift out CARE!!
} 6. Place on an insulating surface and with a knife strike the
} interface between the two stubs - the system will fracture.
} 7. When dried off you have two sets of surfaces to look at in LM or
} SEM.
}
} It works great on a number of differing media other than fibres, the only
} snag is they must not be affected by the water base.
}
} Steve Chapman
} Senior Consultant Protrain
} For professional training in SEM, TEM and EDX world wide
} www.emcourses.com
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Mar 15 11:52:38 2001



From: Karen Schlueter :      kschlueter-at-salinas.ars.usda.gov
Date: Thu, 15 Mar 2001 09:50:49 -0800
Subject: TEM - Liquid Nitrogen storage

Contents Retrieved from Microscopy Listserver Archives
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I need some info on liquid nitrogen dispensing devices. Our EM lab has four
20 liter dewars for liquid nitrogen storage. A piece of styrofoam (2 x 6)
is attached to the lid. We would like to purchase a dispensing device
because it would be easier to use and less waste of liquid nitrogen.
Thanks in advance
Karen Schlueter
PWA, ARS, USDA
EM Lab
1636 East Alisal St.
Salinas, CA 93905
(831) 755-2847
FAX: (831) 755-2814
kschlueter-at-salinas.ars.usda.gov



From daemon Thu Mar 15 12:27:34 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Thu, 15 Mar 2001 13:22:09 -0500
Subject: TEM sample prep question: TEM of dehydrated meat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all

As a materials scientist, I haven't had any experience doing TEM sample prep
of biological samples, so I need some assistance in this.

I've been asked to look at in the TEM some dehydrated beef looking for the
changes in sarcomere length etc due to the dehydration process.

I have at my disposal staining solutions of 0.5% RuO4, and 2% methanolic UA
and need some procedures on how to go about preparing these specimens.
(Things like cut the dehydrated sample into cubes, stain with this for how
long at this temp then embed and microtome or such.)

Thanks in advance.

dz

David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Thu Mar 15 12:43:25 2001



From: Arnold, Jim :      jim.arnold6-at-honeywell.com
Date: Thu, 15 Mar 2001 12:42:44 -0600
Subject: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking to purchase a sputter coater ~$5000. I currently have a
Polaron E5200 that is inoperable. I do Failure Analysis on semiconductors,
probably a 6 inch chamber & Au/Pd target. Can anyone in the list give me
some good tools that work for them.

Anyone familiar with BOC Scancoat Six?


Thanks in advance

Jim Arnold
Senior Quality Technician / Failure Analysis
Honeywell - Microelectronics and Technology Center
Columbia, MD 21045
email: jim.arnold6-at-honeywell.com




From daemon Thu Mar 15 12:52:58 2001



From: Norman_C_Miller-at-raytheon.com
Date: Thu, 15 Mar 2001 12:52:30 -0600
Subject: EDS detector chracterization: sensitivity/efficiency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

For organic contamination, monitoring the ratio of TiKa to TiLa is good because
TiLa is absorbed heavily by organic layers on the detector window. For ice, a
spectrum from say calcium fluoride is good because again fluorine Ka is heavily
absorbed by oxygen containing layers such as ice.

Carl Miller
---------------------- Forwarded by Norman C Miller/RES/Raytheon/US on
03/14/2001 02:00 PM ---------------------------


"Brian Wajdyk" {Brian.Wajdyk-at-onsemi.com} on 03/14/2001 09:40:51 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Norman C Miller/RES/Raytheon/US)


Fellow Microscopists,

Fellow Microscopists,

I want to track the performance of our EDS detector over time. We
currently
check: peak to background ratios via peak-to-tail of Mn k-alpha,
symmetry
via Full Width Tenth Max, peak offset via centeroid of the Mn k-alpha
and
L-alpha to known values, and resolution via Full Width Half Max. What I
don't have is a good way to determine sensitivity of the detector to
determine when to remove ice/hydrocarbon build up. In the past I used a
80%Mn 20%Cu standard and looked at the ratio of Cu k-alpha to Mn l-alpha
because at 10kV accelerating voltage, the peaks are nearly a 1:1 ratio.
Unfortunately I no longer have this standard or the means to acquire a
new
one thanks to the slow down in the e semiconductor industry. My best
guess
would to be to clean the detector then use the k-alpha to l-alpha ratio
of
Cu. However I know that we have the best microscopists in the world at
our
disposal. Any suggestions for sensitivity tracking, or other useful
detector characterizations I may have missed, would be greatly
appreciated.

Sincerely,

Brian Wajdyk
Sr. Electron Microscopist
On Semiconductor
brian.wajdyk-at-onsemi.com
602-244-4883




From daemon Thu Mar 15 13:41:40 2001



From: drose-at-wlgore.com
Date: Thu, 15 Mar 2001 14:23:18 -0500
Subject: Sputter coating: Target thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear List,

I am looking to replace my target on my sputter coater and would like to find
out if there is an optimal thickness for the target. Is it material dependant?
I had requested 2.5mil from the thickness of my old target but the vendor I
spoke with will only source a minimum 4mil thick target. I am looking at Pt and
Au/Pd. Will I have to increase current to get the same deposition rate for a
thicker target?

TIA

David B Rose
W. L. Gore and Associates
297 Blue Ball Road
Elkton, MD 21921
410-506-2958




From daemon Thu Mar 15 15:03:29 2001



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Thu, 15 Mar 2001 15:57:45 -0500
Subject: My LKB microtome repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many thanks to all of you who offered advice, assistance, consolation
and even a loan of the part or a used instrument for parts. I was
able to find a replacement ($100 - overpriced perhaps but worth every
penny!) and will get it installed next week with the directions from
another microscopy list reader.

Again my thanks to all and if anyone wants the names and addresses of
some LKB parts dealers and/or service folks, I will gladly provide
you with my new list off-line.

Regards,

Dick Briggs



From daemon Thu Mar 15 15:23:29 2001



From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Thu, 15 Mar 2001 13:08:07 -0800
Subject: Re: Reichert Frigo-cut repairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some years ago I had Melins (510-234-5749) repair our Reichert 2800. They
insisted on taking it to their shop and it needed a new compressor from
Europe. The bill was over $2000. $130/ hour is a typical charge for this
type of work in this area. When I the Reichert unit was destroyed in a
water flood I purchased a Microm cryostat. It uses standard easily
available refrigeration components. The cost and repair time is gresatly
reduced. Good luck.



At 03:35 PM 3/14/01 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu
http://www.ucsf.edu/janlab


From daemon Thu Mar 15 16:47:15 2001



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Fri, 16 Mar 2001 11:35:54 +1100
Subject: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No, thicker target = longer life, but it makes no difference to
deposition rate, current etc.
Chris


----- Original Message -----
} From: {"drose-at-wlgore.com"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 15, 2001 7:23 PM


No, thicker target = longer life, but it makes no difference to
deposition rate, current etc.
Chris


----- Original Message -----
} From: {"drose-at-wlgore.com"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 15, 2001 7:23 PM


G'day

I have an aging Jeol 840 and the monitor image is getting a bit dim
and noisy to the extent that I have to use large spot sizes that are
damaging some samples! Jeol tell me they cannot supply new
tubes. Does anyone know of compatible tubes that could be
utilised? Is there anything special about SEM monitor tubes?

Thanks
Dave





Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au



From daemon Thu Mar 15 21:25:34 2001



From: Long Miao :      lmiao-at-bio.fsu.edu
Date: Thu, 15 Mar 2001 22:19:51 -0800
Subject: How to stick protein to the microscope slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have some difficulties to stick my protein (Mojor Sperm Protein, a basic
protein from Nematode Sperm ) to the microscope slide, therefore, it is
hard
to perform further perfusion experiment. When I try to do so, I lose
everything. Does anyone have experience in such a manipulation? Any
suggestion is highly appreciated.
Long



----------------------------------------------------------------------

Long Miao Postdoctoral Associate
e-mail: lmiao-at-bio.fsu.edu Dept of Biological Science
Voice: (850)644-9817(W) 334 Bio. Unit1,4370
(850)222-3280(H) Florida State University
FAX : (850)644-0481 Tallahassee, FL32306

----------------------------------------------------------------------




From daemon Thu Mar 15 22:26:42 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, March 15, 2001 10:28 PM
Subject: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The CRTs can be rebuilt for about $600.00 to $800.00 USD by Richardson
Electronics in Georgia.

I have their number & can give it to you offline.

Earl weltmer

----- Original Message -----
} From: "Dave Phelan" {emudp-at-mail.newcastle.edu.au}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 15, 2001 4:35 PM


Try Richardson Electronics http://www.rell.com/ . Have CRT information handy
(labels on the CRT) when calling Richardson Electronic. Good luck.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}


G'day

I have an aging Jeol 840 and the monitor image is getting a bit dim
and noisy to the extent that I have to use large spot sizes that are
damaging some samples! Jeol tell me they cannot supply new
tubes. Does anyone know of compatible tubes that could be
utilised? Is there anything special about SEM monitor tubes?

Thanks
Dave





Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au






From daemon Thu Mar 15 22:54:16 2001



From: Peter Jordan :      emsi-at-pe.net
Date: Thu, 15 Mar 2001 21:23:37 -0800
Subject: Wanted: Zeiss 10C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:
We are lookng to purchase a Zeiss 10C TEM, preferable in working
condition but would consider one needing some work. Please contact us direct
via e-mail or call 909 301-9130 (Los Angeles area)
EMSI
Peter Jordan




From daemon Fri Mar 16 05:42:25 2001



From: drose-at-wlgore.com
Date: Fri, 16 Mar 2001 06:22:29 -0500
Subject: Re: Sputter coating: Target thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Thank you all for your replies. There is certainly a great wealth of
information here.

In summary:

Target thickness does not have a adverse effect on deposition rate.
A thicker target is desirable from a cost to fabricate standpoint.

- david





From daemon Fri Mar 16 06:07:06 2001



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Fri, 16 Mar 2001 14:01:37 +0200
Subject: JEOL 733 - circuit diags XM-XCU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
I'm looking for circuit diags for J-TEC X-ray counting electronic
units XM-XCU. These were supplied with some JEOL 733 'probes although
most seem to have gone out with ORTEC. Can anyone help?
Thanks,
MAlc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Fri Mar 16 06:50:10 2001



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Fri, 16 Mar 2001 07:45:37 -0500
Subject: Re: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Both display and photo-monitor tubes can be recoated. We had our photo CRT
recoated by
Video display corp (Tucker, GA 800-241-5005) in 1993. I don't know if
they're still in business, but they did a good job.
JSIII

} Try Richardson Electronics http://www.rell.com/ . Have CRT information handy
} (labels on the CRT) when calling Richardson Electronic. Good luck.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane, Duluth GA 30096
} (770)232-7785 ph.
} (770)232-1791 fax
}
} This message is made of 100% recycled electrons.
} -----Original Message-----
} } From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} Date: Thursday, March 15, 2001 10:28 PM
} Subject: Jeol 840 viewing screen replacement
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)




From daemon Fri Mar 16 09:49:40 2001



From: Caroline Miller :      camiller-at-creighton.edu
Date: Tue, 16 Mar 1999 09:40:42 -0600
Subject: Durcupan ACM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone use this resin and use it for antibody staining? We will be
using this resin for embedding. Also any suggestions on etching.
Thanks. Caroline Miller



From daemon Fri Mar 16 10:16:53 2001



From: Louis Kerr :      lkerr-at-mbl.edu
Date: Fri, 16 Mar 2001 11:14:00 -0500
Subject: EDS: TN-5400 monitor needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Tracor Northern TN-5402 EDS system mounted on a JEOL SEM. The
monitor just went up in smoke and I need to a) find a replacement or b)
try to replace the hardware. Since I don't have much money and we would
like to replace the whole system in a couple of years I can't spend much
to repair the monitor.

Does anyone have a monitor for sale/offer? Does anyone know of a
replacement monitor available on the market? A standard BNC type of
monitor does not work because of a proprietary modulation. Has anyone
successfully upgraded the hardware minus the detector?

Thanks,
Louie

--
Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu/
http://www.courses.mbl.edu/


From daemon Fri Mar 16 11:08:02 2001



From: Craig Klotz :      cklotz-at-mcw.edu
Date: Fri, 16 Mar 2001 10:57:39 -0600
Subject: Service for MT2B

Contents Retrieved from Microscopy Listserver Archives
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Our Sorvall MT2B is having some problems, to say the least. I would very
much appreciate any info. concerning service of this machine. We are
located in Milwaukee, WI. I have contacted Boeckeler (new owners of RMC)
regarding service. They informed me they are no longer providing service
on these models. What other options do I have?

TIA
Craig M. Klotz, BS,CT(ASCP)
EM Tech II
Neuromuscular Lab
Medical College of Wisconsin
cklotz-at-mcw.edu


From daemon Fri Mar 16 16:53:59 2001



From: Jonathan Wilde :      jonathan-at-interactivedimension.com
Date: Fri, 16 Mar 2001 22:47:06 -0000
Subject: RE: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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} -----Original Message-----
} From: Julian Smith III [mailto:smithj-at-Winthrop.edu]
} Sent: Friday, March 16, 2001 12:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Jeol 840 viewing screen replacement
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Both display and photo-monitor tubes can be recoated. We had
} our photo CRT
} recoated by
} Video display corp (Tucker, GA 800-241-5005) in 1993. I don't know if
} they're still in business, but they did a good job.
} JSIII
}
} } Try Richardson Electronics http://www.rell.com/ . Have CRT
} information handy
} } (labels on the CRT) when calling Richardson Electronic. Good luck.
} }
} } Vitaly Feingold
} } Scientific Instruments and Applications
} } 2773 Heath Lane, Duluth GA 30096
} } (770)232-7785 ph.
} } (770)232-1791 fax
} }
} } This message is made of 100% recycled electrons.
} } -----Original Message-----
} } } From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
} } To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} } Date: Thursday, March 15, 2001 10:28 PM
} } Subject: Jeol 840 viewing screen replacement
} }
} }
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } G'day
} }
} } I have an aging Jeol 840 and the monitor image is getting a bit dim
} } and noisy to the extent that I have to use large spot sizes that are
} } damaging some samples! Jeol tell me they cannot supply new
} } tubes. Does anyone know of compatible tubes that could be
} } utilised? Is there anything special about SEM monitor tubes?
} }
} } Thanks
} } Dave
} }
} }
} }
} }
} }
} } Dave Phelan
} } EM/X-Ray Unit
} } University of Newcastle
} } NSW 2308
} } AUSTRALIA
} } Ph 02 4921 5667
} } Fax 02 4921 7019
} } emudp-at-mail.newcastle.edu.au
}
}
} Julian P.S. Smith III
} Dept. of Biology
} Winthrop University
} Rock Hill, SC 29733
} 803-323-2111 x6427 (vox)
} 803-323-3448 (fax)
}
}
}


From daemon Fri Mar 16 16:54:00 2001



From: Jonathan Wilde :      jonathan-at-interactivedimension.com
Date: Fri, 16 Mar 2001 22:47:00 -0000
Subject: RE: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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list...Take me off the list...Take me off the list...Take me off the list...

} -----Original Message-----
} From: Julian Smith III [mailto:smithj-at-Winthrop.edu]
} Sent: Friday, March 16, 2001 12:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Jeol 840 viewing screen replacement
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Both display and photo-monitor tubes can be recoated. We had
} our photo CRT
} recoated by
} Video display corp (Tucker, GA 800-241-5005) in 1993. I don't know if
} they're still in business, but they did a good job.
} JSIII
}
} } Try Richardson Electronics http://www.rell.com/ . Have CRT
} information handy
} } (labels on the CRT) when calling Richardson Electronic. Good luck.
} }
} } Vitaly Feingold
} } Scientific Instruments and Applications
} } 2773 Heath Lane, Duluth GA 30096
} } (770)232-7785 ph.
} } (770)232-1791 fax
} }
} } This message is made of 100% recycled electrons.
} } -----Original Message-----
} } } From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
} } To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} } Date: Thursday, March 15, 2001 10:28 PM
} } Subject: Jeol 840 viewing screen replacement
} }
} }
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } G'day
} }
} } I have an aging Jeol 840 and the monitor image is getting a bit dim
} } and noisy to the extent that I have to use large spot sizes that are
} } damaging some samples! Jeol tell me they cannot supply new
} } tubes. Does anyone know of compatible tubes that could be
} } utilised? Is there anything special about SEM monitor tubes?
} }
} } Thanks
} } Dave
} }
} }
} }
} }
} }
} } Dave Phelan
} } EM/X-Ray Unit
} } University of Newcastle
} } NSW 2308
} } AUSTRALIA
} } Ph 02 4921 5667
} } Fax 02 4921 7019
} } emudp-at-mail.newcastle.edu.au
}
}
} Julian P.S. Smith III
} Dept. of Biology
} Winthrop University
} Rock Hill, SC 29733
} 803-323-2111 x6427 (vox)
} 803-323-3448 (fax)
}
}
}


From daemon Fri Mar 16 16:54:01 2001



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 16 Mar 2001 16:48:58 -0600
Subject: RE: Double labelling of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Claudia, I would direct you to the March issue of journal of histochemistry
and cytochemistry, they have published a procedure to double-label with gold
at the pre-embedding stages, good luck
Marge

Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation
email: springett.margaret-at-mayo.edu


} ----------
} From: Claudia Hayward-Costa
} Sent: Thursday, March 15, 2001 2:49 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: IEM: Double labelling of cells
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Microscopists,
}
} I was asked to perform double labelling of surface antigens on
} leukaemia cell cultures and cord blood progenitors.
}
} In the past I used a pre-embedding protocol for single labelling
} which worked quite well.
}
} I would like to continue with a pre-embedded protocol, but both
} primary antibodies are raised in the same animal and I wonder how
} I can block unspecific labelling in that case.
}
} (I have a protocol for post-embedded double staining with primary
} AB raised in the same animal, but would prefer to use that as a
} last resort)
}
} So far I used a 10 nm gold F(ab)2- AB - if I use a 5nm gold F(ab)2
} AB will I be able to distinguish them in the TEM?
}
} I would appreciate your input very much.
}
} Many thanks
}
} Claudia
}
}
} Dr. C. Hayward-Costa
} School of Life Sciences
} Kingston University
} Penrhyn Road, Kingston upon Thames
} Surrey KT1 2EE, UK
} 44(0)208 547 2000 x 2240
} Email: c.hayward-at-kingston.ac.uk
}
}


From daemon Fri Mar 16 16:54:01 2001



From: Jonathan Wilde :      jonathan-at-interactivedimension.com
Date: Fri, 16 Mar 2001 22:47:12 -0000
Subject: RE: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Take me off the list...Take me off the list...Take me off the list...Take me
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} -----Original Message-----
} From: Julian Smith III [mailto:smithj-at-Winthrop.edu]
} Sent: Friday, March 16, 2001 12:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Jeol 840 viewing screen replacement
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Both display and photo-monitor tubes can be recoated. We had
} our photo CRT
} recoated by
} Video display corp (Tucker, GA 800-241-5005) in 1993. I don't know if
} they're still in business, but they did a good job.
} JSIII
}
} } Try Richardson Electronics http://www.rell.com/ . Have CRT
} information handy
} } (labels on the CRT) when calling Richardson Electronic. Good luck.
} }
} } Vitaly Feingold
} } Scientific Instruments and Applications
} } 2773 Heath Lane, Duluth GA 30096
} } (770)232-7785 ph.
} } (770)232-1791 fax
} }
} } This message is made of 100% recycled electrons.
} } -----Original Message-----
} } } From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
} } To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} } Date: Thursday, March 15, 2001 10:28 PM
} } Subject: Jeol 840 viewing screen replacement
} }
} }
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } G'day
} }
} } I have an aging Jeol 840 and the monitor image is getting a bit dim
} } and noisy to the extent that I have to use large spot sizes that are
} } damaging some samples! Jeol tell me they cannot supply new
} } tubes. Does anyone know of compatible tubes that could be
} } utilised? Is there anything special about SEM monitor tubes?
} }
} } Thanks
} } Dave
} }
} }
} }
} }
} }
} } Dave Phelan
} } EM/X-Ray Unit
} } University of Newcastle
} } NSW 2308
} } AUSTRALIA
} } Ph 02 4921 5667
} } Fax 02 4921 7019
} } emudp-at-mail.newcastle.edu.au
}
}
} Julian P.S. Smith III
} Dept. of Biology
} Winthrop University
} Rock Hill, SC 29733
} 803-323-2111 x6427 (vox)
} 803-323-3448 (fax)
}
}
}


From daemon Fri Mar 16 16:54:01 2001



From: Jonathan Wilde :      jonathan-at-interactivedimension.com
Date: Fri, 16 Mar 2001 22:46:49 -0000
Subject: RE: Jeol 840 viewing screen replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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} -----Original Message-----
} From: Julian Smith III [mailto:smithj-at-Winthrop.edu]
} Sent: Friday, March 16, 2001 12:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Jeol 840 viewing screen replacement
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Both display and photo-monitor tubes can be recoated. We had
} our photo CRT
} recoated by
} Video display corp (Tucker, GA 800-241-5005) in 1993. I don't know if
} they're still in business, but they did a good job.
} JSIII
}
} } Try Richardson Electronics http://www.rell.com/ . Have CRT
} information handy
} } (labels on the CRT) when calling Richardson Electronic. Good luck.
} }
} } Vitaly Feingold
} } Scientific Instruments and Applications
} } 2773 Heath Lane, Duluth GA 30096
} } (770)232-7785 ph.
} } (770)232-1791 fax
} }
} } This message is made of 100% recycled electrons.
} } -----Original Message-----
} } } From: Dave Phelan {emudp-at-mail.newcastle.edu.au}
} } To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} } Date: Thursday, March 15, 2001 10:28 PM
} } Subject: Jeol 840 viewing screen replacement
} }
} }
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} } G'day
} }
} } I have an aging Jeol 840 and the monitor image is getting a bit dim
} } and noisy to the extent that I have to use large spot sizes that are
} } damaging some samples! Jeol tell me they cannot supply new
} } tubes. Does anyone know of compatible tubes that could be
} } utilised? Is there anything special about SEM monitor tubes?
} }
} } Thanks
} } Dave
} }
} }
} }
} }
} }
} } Dave Phelan
} } EM/X-Ray Unit
} } University of Newcastle
} } NSW 2308
} } AUSTRALIA
} } Ph 02 4921 5667
} } Fax 02 4921 7019
} } emudp-at-mail.newcastle.edu.au
}
}
} Julian P.S. Smith III
} Dept. of Biology
} Winthrop University
} Rock Hill, SC 29733
} 803-323-2111 x6427 (vox)
} 803-323-3448 (fax)
}
}
}


From daemon Fri Mar 16 22:02:28 2001



From: Wentao Qin :      wentao-at-newton.umsl.edu
Date: Fri, 16 Mar 2001 21:44:38 -0600 (CST)
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Sat Mar 17 12:10:58 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 17 Mar 2001 12:02:46 -0600
Subject: Educational Outreach: Sands from around the World

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

As many of you know, I maintain the WWW pages for
Project Micro which is dedicated to Educational Outreach to pre-college
students. One of their projects is sand from around the world which
is used in the classroom activities section.

Today I uploaded their latest web page documenting their sand collection
administered by Joe Neilly of Abbott Labs . You can see this page at

http://microscopy.com/ProjectMicro/SandCollection.html

I could not help to notice that their supply of sands from outside the
USA has become sorely lacking. In particuliar

Europe, Africa and South America

are now all completely depleted. And

Australia and Asia

only have supplies from only one location left.

Can I suggest to members of this list particularly if you are
not from the USA, that if you have the opportunity this would
be an excellent and simple way that you can contribute to the
education of our next generation of microscopist's. The sand
is available to any educator, regardles of their affiliation and
location.

All the information you need to contribute "sand" from your local
beach is listed on that WWW page as well as the end of this message.

It will cost you a few $$ out of your pocket to send the sand ,
but remember, it is for a good cause. So spend a few minutes next time
you drive past a beach and fill up a small plastic bag, and send it to Joe.


Cheers....
Nestor
Your Friendly Neighborhood SysOp.


---------------------------
Here is an exerpt from the Sands WWW page

To Request Sand

Select the sands you would like from the list below and e-mail your request to
joe.neilly-at-abbott.com. Include your selection (limit 6 per request), where
the sands should be
sent, and how you plan to use them. This collection was created for
Microscopic Explorations but
how you ultimately use them is up to you. Great stories and photos about
how you used the
sands are always welcome!

To Donate Sand

Sand donations are what keeps this collection going. All donations are
appreciated and will be
shared with many educators. To send your sand, fill a Ziplock sandwich bag
half full and place it
in another Ziplock bag. Mail your donation to:

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202




From daemon Sat Mar 17 14:04:28 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Sat, 17 Mar 2001 21:00:09 +0100
Subject: Microwave polymerisation of LR-White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,
recently there was a discussion about the stability of LR-White in the EM-beam. One distribtion mentioned a polymerization of LR-White by microwave treatment. I don´t remember who sent this mail.
I would be interested to test this procedure.
Who knows how to do this kind of LRW polymerization? What is the set-up?
How long does it take, how much Energy (Watt) is needed?
Maybe the colleague who posted this mail remembers the discussion and is able to give some information.

Thanks in advance,
with best regards,
Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________________
Alles unter einem Dach: Informationen, Fun, E-Mails. Bei WEB.DE: http://web.de
Die große Welt der Kommunikation: E-Mail, Fax, SMS, WAP: http://freemail.web.de



From daemon Sat Mar 17 18:52:51 2001



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 17 Mar 2001 18:47:24 -0600
Subject: Microwave polymerization of LRW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Michael:
The following procedure for polymerization of LR-White has worked of me in
my microwave oven: I will start with the infiltration phase: The wattage
setting I used was about 700-750 watts.

1:1 95% ETOH / LR-White 50'C temperature restriction setting (trs) x2 for
10 minutes each.

100% LR-White 50'C (trs) x3 for 10 minutes each.

Polymerization in the oven: In this phase be sure to fill the beem capsule
to the very top and cap off with parafilm to make a tight seal to prevent
water from getting into the resin. Submerge the capsules in a water bath and
place the temperature probe in the bath to monitor the temp.

100% resin, 60'C (trs) x1 for 10 minutes.
" " 70'C (trs) x1 " " "
" " 80'C (trs) x1 " 20 minutes

The blocks will be firm and will cut very well.
For more information on this technique you can contact Rick Giberson at TED
PELLA CORP. USA using their toll free number, I don't know what that is in
Germany! He can give you many more details about LR-White and the microwave
oven. I understand that lesser wattage setting can be used to infiltrate and
polymerize LR-White but I have not experimented with them as yet!

Good Luck

Ron Austin (Research Associate)
Dept. of Pathology
L.S.U. Medical Ct.
Shreveport, LA
318-675-4775
rla-at-mindspring.com



From daemon Sun Mar 18 01:50:27 2001



From: toner2-at-atozasia.com
Date: Sat, 17 Mar 01 05:38:16 EST
Subject: toner supplies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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ITEM #11 LASERJET SERIES 4000 (27X)-------------------------$72
ITEM #12 LASERJET SERIES 3SI/4SI (91A)--------------------$54
ITEM #13 LASERJET SERIES 4, 4M, 5,5M-----------------------$49

HEWLETT PACKARD FAX (ON PAGE 2)

ITEM #14 LASERFAX 500, 700 (FX1)----------$49
ITEM #15 LASERFAX 5000,7000 (FX2)------$54
ITEM #16 LASERFAX (FX3)------------------------$59
ITEM #17 LASERFAX (FX4)------------------------$54

LEXMARK/IBM (ON PAGE 3)

OPTRA 4019, 4029 HIGH YIELD---------------$89
OPTRA R, 4039, 4049 HIGH YIELD---------$105
OPTRA E----------------------------------------------------$59
OPTRA N--------------------------------------------------$115
OPTRA S--------------------------------------------------$165
-
EPSON (ON PAGE 4)

ACTION LASER 7000,7500,8000,9000-------$105
ACTION LASER 1000,1500-------------------------$105

CANON PRINTERS (ON PAGE 5)

PLEASE CALL FOR MODELS AND UPDATED PRICES
FOR CANON PRINTER CARTRIDGES

PANASONIC (0N PAGE 7)

NEC SERIES 2 MODELS 90 AND 95----------$105

APPLE (0N PAGE 8)

LASER WRITER PRO 600 or 16/600------------$49
LASER WRITER SELECT 300,320,360---------$74
LASER WRITER 300 AND 320----------------------$54
LASER WRITER NT, 2NT------------------------------$54
LASER WRITER 12/640--------------------------------$79



CANON FAX (ON PAGE 9)

LASERCLASS 4000 (FX3)---------------------------$59
LASERCLASS 5000,6000,7000 (FX2)---------$54
LASERFAX 5000,7000 (FX2)----------------------$54
LASERFAX 8500,9000 (FX4)----------------------$54


CANON COPIERS (PAGE 10)

PC 3, 6RE, 7 AND 11 (A30)---------------------$69
PC 300,320,700,720 and 760 (E-40)--------$89

IF YOUR CARTRIDGE IS NOT LISTED CALL CUSTOMER SERVICE AT 1-888-248-2015

90 DAY UNLIMITED WARRANTY INCLUDED ON ALL PRODUCTS.

ALL TRADEMARKS AND BRAND NAMES LISTED ABOVE ARE PROPERTY OF THE
RESPECTIVE HOLDERS AND USED FOR DESCRIPTIVE PURPOSES ONLY.



From daemon Sun Mar 18 12:04:16 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sun, 18 Mar 2001 11:59:57 -0600
Subject: Sand Contributions: Nestor Errors...!!!

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Colleagues

A number of people have reminded me about the growing
problems with hoof and mouth disease around the world
and that sending possible organic material from country
to country should not be considered at this time.

I humbly acknowledge that you are all absolutely correct!

It simply did not occur to me to connect sand with this problem.
I guess my physics background is just showing it's
tunnel vision, since I think of sand as inorganic compounds
and neglect to think of organic "contaminants" which might be
included.

For the moment it would therfore certainly be prudent to hold off collecting
and/or send and sand samples for the Micro Project, even if it can
be documented that the samples have been properly sterilized.
Let's wait until this potential problem is controlled. It is better
to error on the safe side.

Thanks again, to everyone that pointed out my error.

Nestor
Your Friendly Neighborhood SysOp.







From daemon Mon Mar 19 06:44:09 2001



From: Veys Pascal :      Veys-at-bota.ucl.ac.be
Date: Mon, 19 Mar 2001 13:37:23 +0100
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



==============================
Dr Pascal VEYS
Laboratory of Plant Biology
Faculty of Sciences
Catholic University of Louvain

Place Croix du Sud 5 bte 14
B-1348 Louvain-la-Neuve
Belgium
Phone: +32/10/473004
Fax: +32/10/473471
E-mail: Veys-at-bota.ucl.ac.be
==============================



From daemon Mon Mar 19 06:53:41 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Mon, 19 Mar 2001 09:49:24 -0300
Subject: LM: lignin extraction in seeds

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,
I am working with Ilex paraguariensis seeds. After dissection, with
mechanical extraction of the endocarp, the micropylar endosperm is enveloped
by a lignified cap. This one do not allow the cellular visualization, in
clarified material (Herr´s method), of the endosperm because the yellow
color of the lignin. Is there a non aggressive protocol for lignin
extraction?
Thank´s.

Rinaldo Pires dos Santos

Dr. Rinaldo Pires dos Santos - e-mail: rinaldop-at-uol.com.br
Lab. of Plant Anatomy - Dept. of Botany - UFRGS
Porto Alegre - RS - Brazil




From daemon Mon Mar 19 07:45:25 2001



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 19 Mar 2001 09:44:43 -0400
Subject: dental amalgam in SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

Well, I stepped in it this time. I agreed to do some "gee-whiz" shots
for my dentist of a tooth with a rather large amalgam filling. Only
later
did it sink in that the mercury might be a problem under vacuum and the
beam. Has anybody out there done this? I'd be looking at the thing with
gold coating an a fairly light beam (say 10 kV), but unfortunately, not
with
a cold stage. I've checked the archives and found one brave soul who
said
that he's done this on old amalgams. I can't really say how old this one
is, but
I'm pretty sure it wasn't made yesterday. Call me paranoid, but I'd like
some
more opinions before I do something really stupid!

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Mon Mar 19 09:06:50 2001



From: Brian J Laughlin :      brjlau18-at-US.ibm.com
Date: Mon, 19 Mar 2001 10:01:31 -0500
Subject: TEM: Automated Prep Tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to evaluate some tools that perform automated TEM sample prep
prior to FIB thinning. I am looking for some one that has experience using
Sela microcleaving tools in conjuction with the TEMstation. I work with
failure and material analysis in the IC industry so submicron accuracy and
relaibility are important concerns of mine. Also, we will soon be facing
chips with low K dielectrics which from what I have heard are extreme soft
and hard to polish. I am not sure if the sawing technique they employ will
be able to section this type of material.

If anyone on this list has any experience with these tools and can give me
insight into their worth as a prep technique please get in touch with me.

Sincerely,

Brian Laughlin

FIB/TEM Engineer
IBM, Burlington, VT
Microelectronics Division
Surface and Materials Science Laboratory (Dept. GP8)
bjlau18-at-us.ibm.com

Lab: Bldg. 967-1 N18, (802) 769-2865
Office: (802) 769-5224
Fax: (802) 769-1220
Mail: IBM Burlington, 1000 River St., Essex Junction, VT 05452 Mailstop
967L




From daemon Mon Mar 19 10:39:58 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Mon, 19 Mar 2001 11:40:07 -0500
Subject: Liquid helium TEM cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are interested in buying a liquid helium TEM cold stage. Has anyone had
experience with it and how was it? Is there any other companies selling
this apart from Gatan?

I would appreciate very much for any input on this.

Best regards
Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Mon Mar 19 13:01:50 2001



From: semcore-at-audumla.mdacc.tmc.edu
Date: Mon, 19 Mar 2001 12:57:04 -0600
Subject: Decalcifivation of bone for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a step by step protocol for decalcifying bone for
transmission electron microscopy using EDTA?

Thanks,

Kenn






Dr. Corazon D. Bucana, Ph.D.
Mr. Kenneth Dunner, Jr.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
High Resolution Electron Microscopy Facility
7777 Knight Road, Box 173
Room SRB 1.660
Houston, Texas 77054
PH: 713-792-8106
FAX:713-792-8747


From daemon Mon Mar 19 13:58:17 2001



From: Edsworth-at-aol.com
Date: Mon, 19 Mar 2001 14:54:15 EST
Subject: JEOL 733

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in need of a gas flow proportional counter (GFPC) for a JEOL 733
microprobe. Alternately, I may also need some W wire of the appropriate size
to restring an existing unit. Thanks for any help/advice.

Ed Holdsworth
General Mgr.
SEMTEC Laboratories, Inc.


From daemon Mon Mar 19 14:56:23 2001



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Mon, 19 Mar 2001 15:51:49 -0500 (EST)
Subject: Re: Decalcifivation of bone for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kenn,

I have had good luck decalcifying bone for TEM using this EDTA protocol:

Fix 1-2 mm sized bone pieces for two days in 2.5 percent glutaraldehyde in
0.1 M Sorensen's buffer.

Decalcify on a shaker for 3-7 days in 7.5 % disodium EDTA, 2.5 % glut. in
0.1 M Sorensen's buffer. (pH the decal solution to physiological pH with
NaOH.)

Change to fresh decal solution every couple of days.

Check for complete decalcification by taking before and after X-rays of
the tissue.

Rinse twice in buffer

Post fix in 1 % osmium in buffer.

Rinse once with buffer, then once with ddH2O

en bloc stain for 1 hour with aqueous 3% uranyl acetate.

Dehydrate in a graded series of EtOH, infiltrate, and embed in epon.


Good luck, and I hope this helps.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Mon, 19 Mar 2001 semcore-at-audumla.mdacc.tmc.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have a step by step protocol for decalcifying bone for
} transmission electron microscopy using EDTA?
}
} Thanks,
}
} Kenn
}
}
}
}
}
}
} Dr. Corazon D. Bucana, Ph.D.
} Mr. Kenneth Dunner, Jr.
} Department of Cancer Biology
} U.T. M.D. Anderson Cancer Center
} High Resolution Electron Microscopy Facility
} 7777 Knight Road, Box 173
} Room SRB 1.660
} Houston, Texas 77054
} PH: 713-792-8106
} FAX:713-792-8747
}



From daemon Mon Mar 19 15:34:18 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 19 Mar 2001 15:32:57 -0600
Subject: Micro-g air table part

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I'm looking for a part no longer available from the manufacturer. We
have a TMC Micro-g air table, and one of the black plastic parts that
holds together the leveling valve (at the ends of the table) has
broken.

I can't get one of these from TMC anymore -- the whole valve kit has
to be bought for $120, and the bloody things were special-made for
TMC.

Does anyone perchance have any of these things that they no longer need?

Thanks to all.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Mar 19 15:48:31 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 19 Mar 2001 16:53:14 -0500
Subject: TEM screens recoated - Grant Scientific?

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
I need to have TEM screens recoated and heard that Grant Scientific is the
place to contact. I called 803-799-6716 (a # I found in my address
archives) and got the lovely fax machine scream sound.
any help?
thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Mon Mar 19 16:13:11 2001



From: SMancuso-at-specialmetals.com
Date: Mon, 19 Mar 2001 17:06:22 -0500
Subject: Re: dental amalgam in SEM?

Contents Retrieved from Microscopy Listserver Archives
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I have examined amalgams in the SEM in the past. There is little problem
with the mercury as it is amalgamated with silver.
I have even used pure mercury in the SEM as a standard for WDS with no
problem. I believe mercury is used in high vacuum
diffusion pumps.

Good luck!

Sam O. Mancuso
Group Leader, Physical Metallurgy
Special Metals Corporation
New Hartford NY 13413
phone: (315) 798-2920
fax: (315) 798-2001



From daemon Mon Mar 19 17:56:38 2001



From: Frank Lee :      flee-at-uhnres.utoronto.ca
Date: Mon, 19 Mar 2001 17:55:01 -0600
Subject: LM: Question: Objective calibration/fluorescence halo

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I've got 2 really basic questions. First, I'm trying to determine the
actual magnification power of my objectives. I'm using a calibration
slide with 0.01 mm (I think that's the increments). What are the
procedures for using this slide? I'm assuming I need to find out the
diameter of the field and then do a reverse calculation. For example, if
I have 36 lines (0.36 mm), using a 40x objective coupled with a 10x
ocular, how do I calculate the actual mag power of my 40x obejctive.
My second question is about fluorescent microscopy. I'm using a Zeiss
fluorescence microscope with 40x and 63x Zeiss "neofluro" objectives,
when I'm in focus, there are halos around the fluorescence image. What
does this mean? Are my lens dirty?
Oh, and one last thing. Does anyone know any good websites that teaches
the basics about microscope maintenance and general troubleshooting faq?

Thanks for your help.

Frank




From daemon Mon Mar 19 19:15:09 2001



From: Todd Clason :      clason-at-u.washington.edu
Date: Mon, 19 Mar 2001 17:09:10 -0800
Subject: LM question: Suggestions for optical reconditioning?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all,

I have a wonderful old Leitz Ortholux which I use to look at diatoms in my
off time. Unfortunately, the coating on one of the prisms in the trinocular
head has degraded with time, and shows up as a mottled shadow when viewing
specimens.

Does anyone have recommendations and/or know of resources for reconditioning
optics? Any suggestions concerning parts suppliers for old microscopes would
be appreciated as well.

Thanks so much for your time!

Todd
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Todd A. Clason, Imaging Coordinator
Department of Zoology
University of Washington
Box 351800
Seattle, WA 98195-1800

A087 PAB
clason-at-u.washington.edu
Tel: (206) 685-1519
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Mon Mar 19 22:07:24 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Mon, 19 Mar 2001 23:06:23 -0500
Subject: Re: TEM screens recoated - Grant Scientific?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Beth:

From Switchboard (www.switchboard.com):

Grant Scientific Corp
1385 Rock Island Rd,
Gilbert, SC 29054-8821
Phone: (803) 892-2841

Hope that's the right one.

Regards,

Rick Powell


} Hi all,
} I need to have TEM screens recoated and heard that Grant Scientific is the
} place to contact. I called 803-799-6716 (a # I found in my address
} archives) and got the lovely fax machine scream sound.
} any help?
} thanks,
} Beth
}
} **************************************
} Beth Richardson
} EM Lab Coordinator
} Botany Department
} University of Georgia
} Athens, GA 30602
}
} Phone - (706) 542-1790
} FAX - (706) 542-1805
} Email - beth-at-dogwood.botany.uga.edu
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **************************************

*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (919)
845-6324

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Mon Mar 19 23:29:38 2001



From: JNDKepnerLovers-at-aol.com
Date: Mon, 19 Mar 2001 23:28:09 -0600
Subject: Ask-A-Microscopist: GUM MEDIA

Contents Retrieved from Microscopy Listserver Archives
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Colleagues

I've never heard of "Gum Media" can someone answer this question?

Nestor

---------------------------------------------------------------------------

Email: JNDKepnerLovers-at-aol.com
Name: Josh Kepner

Education: 9-12th Grade High School

Location: City, State, Country

Question: We recently bought a home microscopy kit and some "GUM MEDIA" was
included in the set but was not explained in the manual. What is gum media
and how would we use it? HELP!?

---------------------------------------------------------------------------




From daemon Tue Mar 20 03:18:10 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Tue, 20 Mar 2001 03:12:24 -0600
Subject: RE: JEOL 733

Contents Retrieved from Microscopy Listserver Archives
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Osram Sylvania (http://www.sylvania.com) is a major manufacturer of
tungsten wires. If you contact their local sales branch and request a
small sample of the appropriate wire or range of wires, you'll get a
lifetime supply. I've used their wire myself on XRF detectors and found
them at least as good as the manufacturer's.

I can't think of many reasons why the whole detector should ever have to be
replaced. You may need to clean it real well and change windows and wire.
Any other parts that are damaged should be fairly easy to replace or
duplicate.


On Monday, March 19, 2001 1:54 PM, "Edsworth-at-aol.com"-at-sparc5.microscopy.com
[SMTP:"Edsworth-at-aol.com"-at-sparc5.microscopy.com] wrote:
}
}
} I am in need of a gas flow proportional counter (GFPC) for a JEOL 733
} microprobe. Alternately, I may also need some W wire of the appropriate
size
} to restring an existing unit. Thanks for any help/advice.
}
} Ed Holdsworth
} General Mgr.
} SEMTEC Laboratories, Inc.
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Tue Mar 20 03:31:06 2001



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Tue, 20 Mar 2001 09:27:05 +0000
Subject: Re: Liquid helium TEM cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Yan Xin,

We have a liquid helium cold stage made by Oxford Instruments. It
is used rarely because the safe handling precautions needed when
using liquid helium (not to mention the cost) put many people off.
However, if temperatures as low as 6K are a requirement in your
experiment and the relevant safety precautions are taken, the thing works well.
We also have Gatan's liquid nitrogen cold stage, which is much easier
to use although only capable of minus 196C(?).

Hope this helps.

Kind regards,

Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365 Mob: 07796 055149
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************


From daemon Tue Mar 20 05:55:41 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 20 Mar 2001 12:00:07 +0000 (GMT Standard Time)
Subject: Re: Liquid helium TEM cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Yan Xin,

We have an Oxford Instruments double tilt He holder that
fits both out JEOL 3000F and our 2010. We bought it about 2
years ago but Oxford Instruments have since been bought out
by Gatan. We use the holder for a few days every couple of
months and provided that you have common sense the hazards
associated with handling liquid He are not a concern.

We are happy with the performance of the holder. Before
buying we tested both the ultra-low temperature (5K) and
low temperature (15K) versions and bought the 15K version
as it looked more robust and we did not think that there
was any real advantage to us to go to 5K. The holder we
have gets below 10K.

It takes approximately 45 minutes to fill from room
temperature and 30 minutes to refill when cold. If we
operate at 20K it will hold the temperature for about 2
hours.

The tilt drives still work well at 10K and the image is
stable enough to see gold fringes (0.2nm), after the
cooling drift has stopped.

Since Gatan bought out Oxford Instruments I can only think
of Fischione as a possible alternative supplier. It really
is a shame that competition has reduced in the EM accessory
field.

Good luck

Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Mar 20 06:24:10 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 20 Mar 2001 06:24:55 -0600
Subject: Fwd: PhD positions available in the UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


forwarded by request from the confocal list:

} Investigations of biomolecular interactions and mechanics (4 studentships)
} These projects will employ SPM and state-of-the-art computational
} methods to investigate the origins of biomolecular recognition and
} protein folding or RNA folding pathways. Specifically, SPM will be
} employed to characterize interaction forces between complementary
} biological molecules e.g. individual coiled-coil proteins or
} RNA-protein interactions (2 studentships). In parallel, two
} studentships will aim to develop complementary computational methods
} to further investigate the molecular origins of the obtained forces,
} and also to explore the energetics of RNA/protein folding pathways.
}
} Ultra-high resolution studies of surface-chemistry and protein
} structure (2 studentships)
} In collaboration with Professor Richard James (Clinical Laboratory
} Sciences), one project will utilize state-of-the-art SPM methods to
} investigate, in-situ, the structure-function relationships of the
} colicin class of bacterial membrane proteins. A second project will
} aim to image foliate surfaces with high-resolution, and to
} investigate the influence and surface distribution of various
} agrochemicals (in collaboration with Syngenta-agrochemicals)
}
} The development and analysis of surface engineered biomimetic
} systems for drug-delivery & tissue engineering (3 studentships)
} In collaboration with the School's drug-delivery and tissue
} engineering group, these projects will aim to develop novel
} biomimetic polymeric surfaces for drug-delivery. In addition the
} projects will employ state-of-the-art analytical techniques for
} their surface-chemical characterization of polymer systems,
} including SPM, contact angle, X-ray photoelectron microscopy and
} time-of-flight secondary ion mass spectrometry.
}
} Single molecule optical microscopy using a single-molecule light
} source (1 studentship)
} This project will aim to address the current limitations of
} near-field scanning optical microscopy, by replacing the physical
} aperture by a small number of fluorescent molecule at the probe (as
} proposed by W Heckl). The developed technology will be employed for
} the high-resolution analysis of microfabricated biomolecular arrays.
}
} ________________________________________
} Phil Williams
} Laboratory of Biophysics and Surface Analysis
} School of Pharmaceutical Sciences and The Pharmacy School
} University of Nottingham NG7 2RD
} tel: +44 (0)115 9515025
} fax: +44 (0) 115 9515110
} http://www.nottingham.ac.uk/lbsa
}


From daemon Tue Mar 20 07:15:49 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 20 Mar 2001 07:08:18 -0600 (CST)
Subject: Antivibration tables for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for quotes/sources for an anitivibration table for a
TEM. The table will have to be larger than normal to accomodate
some other instrumentation.

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Tue Mar 20 07:33:39 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Tue, 20 Mar 2001 07:23:57 -0600
Subject: RE: dental amalgam in SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have imaged OLD amalgam without problem. FWIW, A copy of the image can
be seen on my web site. No experience with "fresh" material.

http://woody.white.home.att.net


Woody
--------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi listers,
}
} Well, I stepped in it this time. I agreed to do some "gee-whiz" shots
} for my dentist of a tooth with a rather large amalgam filling. Only
} later
} did it sink in that the mercury might be a problem under
} vacuum and the
} beam. Has anybody out there done this? I'd be looking at the
} thing with
} gold coating an a fairly light beam (say 10 kV), but
} unfortunately, not
} with
} a cold stage. I've checked the archives and found one brave soul who
} said
} that he's done this on old amalgams. I can't really say how
} old this one
} is, but
} I'm pretty sure it wasn't made yesterday. Call me paranoid,
} but I'd like
} some
} more opinions before I do something really stupid!
}
} Cheers,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}
}
}


From daemon Tue Mar 20 07:57:43 2001



From: ken.andrew-at-bigpond.com.au
Date: Tue, 20 Mar 2001 07:56:44 -0600
Subject: Ask-A-Microscopist: Converting Optical Microscopes monocular to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


---------------------------------------------------------------------------

Email: ken.andrew-at-bigpond.com.au
Name: Ken ANDREW

Education: Graduate College

Location: Melbourne Australia

Question: Can I convert a Zeiss Jena polarized microscope from monocular to
binocular. I have a student model from late-70's-early 80's which happens
to also have metallographic attachment. E-bay often features binocular
eyepieces. Is the barrel fitting common to all brands or just zeiss jena ?
Does being a polarized scope make any difference ? I wear spectacles.

---------------------------------------------------------------------------




From daemon Tue Mar 20 08:04:30 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 20 Mar 2001 09:00:39 -0500
Subject: Hi Resolution Image of the MSA Logo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there I am looking for a high resolution image of the MSA Logo in either
Freehand, Illustrator or high resolution TIFF or JPEG, dows anyone have one
or does anyone know who does have one? Many thanks.


--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"




From daemon Tue Mar 20 08:05:06 2001



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Tue, 20 Mar 2001 15:01:38 +0100
Subject: 5th Multinational Congress on Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

I would like to inform you that the new version of the homepage of the 5th
Multinational Congress on ELectron Microscopy, that will be held in Lecce,
Italy, from September 20-25 2001, is now ready and available at the URL:
www.MCEM5.unile.it. In the "satellite events" section of the page, you will
also find details about the "International School on Advances in Electron
Microscopy in Materials Science" that will be held before the Congress.

The second circular/call for papers will be distributed to all who
pre-registered very shortly.

Up to now we have 300 preregistrations from all over the world, and we
really hope to have a nice and succesfull meeting.

Hope to see many of you in Lecce.

Best regards

Massimo


Dr. Massimo Catalano
President of MCEM5
CNR-IME
Via Arnesano
73100 Lecce - ITALY
massimo.catalano-at-ime.le.cnr.it



From daemon Tue Mar 20 08:08:41 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 20 Mar 2001 09:04:48 -0500
Subject: Sputter Coater recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I am looking for a new sputter coater to coat specimens for our FEG SEM,
I was thinking of a Chromium coater but they are expensive and the films
don¹t last very long (apparently). Is there a coater available that gives
you small grain size and film longevity? Also are there brands who I should
avoid for poor quality? Please reply off line as such opinions could be
considered hurtful and libelous (even if preceded by IMHO!)
ADVthanksANCE

-

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"




From daemon Tue Mar 20 08:11:09 2001



From: JHumenansky-at-phi.com
Date: Tue, 20 Mar 2001 08:05:55 -0600
Subject: Oxford/ISIS Disk Inspector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd appreciate hearing from users of Disk Inspector software in the ISIS
platform by Oxford. I'm especially interested in manual analysis of
particles detected that are smaller than the minimum size established in
the particle detection setup menu. Hope to share experiences and weed out
some potential problems.

TIA

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Tue Mar 20 08:31:48 2001



From: Ramin Rahbari :      RRahbari-at-synapticcorp.com
Date: Tue, 20 Mar 2001 09:23:13 -0500
Subject: LM: Question: Objective calibration/fluorescence halo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Frank,
Lets take your questions in reverse. It is likely that your 63x lens is an
oil objective. The halo you see is most likely left over oil immersion
media used on the lens. It is obligatory to use the oil for correct image
formation. You indicate the 40x is presenting with the same symptoms, it
doesn't necessarily have to be an oil objective however. It is possible it
was designed as a dry lens but was accidentally smeared with oil.
Specifications for each objective are on the body of the objective. oil
lenses usually have a black colored indicator marking. Cleaning with
non-abrasive lens paper (not chemwipes) and Windex wouldn't hurt either
objective.
You also need to make sure your light source, most likely mercury, is
properly aligned. You may need help with this step, Zeiss manuals usually
mention the steps required, but I strongly urge you seek out an experienced
microscopist for this step.
Actual magnification is a slightly tricky subject. Ocular x objective is a
good range. It is further complicated by additional light gathering /
magnification devices, i.e. field lenses, and the final form of the image.
If you use 35mm film, print the micrograph, and measure your stage
micrometer with a standard ruler to get a ratio which will indicate your
final magnification. This figure will include any incidental magnification
due to any other in line processes. If you are capturing video or digital,
assign xx number for pixels to the stage micrometer.
Good luck
Ramin

-----Original Message-----
} From: Frank Lee [mailto:flee-at-uhnres.utoronto.ca]
Sent: Monday, March 19, 2001 6:55 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

I've got 2 really basic questions. First, I'm trying to determine the
actual magnification power of my objectives. I'm using a calibration
slide with 0.01 mm (I think that's the increments). What are the
procedures for using this slide? I'm assuming I need to find out the
diameter of the field and then do a reverse calculation. For example, if
I have 36 lines (0.36 mm), using a 40x objective coupled with a 10x
ocular, how do I calculate the actual mag power of my 40x obejctive.
My second question is about fluorescent microscopy. I'm using a Zeiss
fluorescence microscope with 40x and 63x Zeiss "neofluro" objectives,
when I'm in focus, there are halos around the fluorescence image. What
does this mean? Are my lens dirty?
Oh, and one last thing. Does anyone know any good websites that teaches
the basics about microscope maintenance and general troubleshooting faq?

Thanks for your help.

Frank




From daemon Tue Mar 20 08:36:55 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 20 Mar 2001 08:33:12 -0600
Subject: Re: Ask-A-Microscopist: GUM MEDIA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:28 PM 3/19/01 -0600, you wrote:
} I've never heard of "Gum Media" can someone answer this question?

It must be "gum arabic", the water-soluble plant-based gum, for
mounting specimens or even slide labels.

There are several mixtures that can be made with it. One comes to
mind that involves chloral hydrate - but perhaps they don't let
high school students work with that.

http://www.nmnh.si.edu/iz/copepod/techniques.htm has some recipes.

- John

} ---------------------------------------------------------------------------
}
} Email: JNDKepnerLovers-at-aol.com
} Name: Josh Kepner
}
} Education: 9-12th Grade High School
}
} Location: City, State, Country
}
} Question: We recently bought a home microscopy kit and some "GUM MEDIA" was
} included in the set but was not explained in the manual. What is gum media
} and how would we use it? HELP!?
}
} ---------------------------------------------------------------------------



From daemon Tue Mar 20 09:24:47 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Tue, 20 Mar 2001 11:22:59 -0400
Subject: Gold target for Edwards 306A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a source for either an actual gold sputter target or
plating service for our gold target for our Edwards 306A coater.
Alternatively a source for gold foil would do. As usual time is of the
essence. Vendors welcome. Thanks.

David O'Neil
Institute for Marine Biosciences
National Research Council
1411 Oxford St.
Halifax , NS B3H 3Z1
Canada
ph. 902-426-8258
fax. 902-426-9413
david.o'neil-at-nrc.ca


From daemon Tue Mar 20 09:32:22 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 20 Mar 2001 08:22:33 -0700
Subject: Re: dental amalgam in SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mercury was indeed used in diffusion pumps, but as far as I know, that has
been replaced by turbo pumps in most cases, especially due to the health
hazards of mercury vapor.

As far as I know (and I am not a doctor!) mercury is fairly harmless in
liquid form. It's the vapor that is dangerous. The problem is, that tiny
drops of mercury tend to evaporate (due to surface tension), so you don't
want to spill mercury. Keeping it in a bottle is not such a problem, as the
vapor pressure is very low and it does not evaporate much.

Amalgam, however, is a different material. As Sam says, it's mercury
amalgamated with silver. To find out what happens upon heating, you should
probably consult some tables.

I heard, that the biggest health problem with Amalgam in teeth did not occur
in patients, but in dentists. They had to handle the mercury and amalgamate
it with silver all the time. Of course, there have been reports about health
risks to patients also, which is probably why I haven't received any Amalgam
fillings for a number of years (?).

If I had to look at a tooth with a filling, I would probably try to avoid
heating it too much by keeping the beam current low.

Michael Bode

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: "SMancuso-at-specialmetals.com"-at-sparc5.microscopy.com
[mailto:"SMancuso-at-specialmetals.com"-at-sparc5.microscopy.com]
Sent: Monday, March 19, 2001 3:06 PM
To: Microscopy Listserv



I have examined amalgams in the SEM in the past. There is little problem
with the mercury as it is amalgamated with silver.
I have even used pure mercury in the SEM as a standard for WDS with no
problem. I believe mercury is used in high vacuum
diffusion pumps.

Good luck!

Sam O. Mancuso
Group Leader, Physical Metallurgy
Special Metals Corporation
New Hartford NY 13413
phone: (315) 798-2920
fax: (315) 798-2001



From daemon Tue Mar 20 09:32:25 2001



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Tue, 20 Mar 2001 09:24:41 -0600
Subject: TEM Reembedding Spurr's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Fellow TEM netter's, HELP!!!

I need some ideas on how to reembed kidney tissue that has been embedded
in Spurr's. I've heard of a method of using propylene oxide. Are there any
other
methods out there in TEM land???

Thanks in advance,

Donald G. Awbrey, HT(ASCP) QIHC
Image Analysis / Electron Microscopy
donaldawbrey-at-texashealth.org





From daemon Tue Mar 20 09:52:58 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Tue, 20 Mar 2001 10:57:33 -0500
Subject: thanks - Grant Scientific info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all the replies about Grant Scientific!
I appreciate y'all!
best regards,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Tue Mar 20 09:56:59 2001



From: Judy Bowen :      jabowen-at-buckman.com
Date: Tue, 20 Mar 2001 09:51:55 -0600
Subject: finding a book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It appears that Barnes and Noble (www.bn.com), Fatbrain (www.fatbrain.com)
and Borders (www.borders.com) have it in stock. However, Amazon.com lists
it as out of print.

Bookfinder.com is usually a good place to look for new and used books.
However, for this book there were very few hits and it appears to list the
author as Oliver Flint, but the ISBN number is the same as the book by Olga
Flint.

Hope you find the book. I have it sitting on my desk now on interlibrary
loan. I was wanting to look at it to determine if I would find it useful.

Judy Bowen
Buckman Laboratories
Memphis, TN USA


Previous message:
I have been looking for a copy of "Food Microscopy", by Olga Flint. It is
one
volume of the Royal Microscopy Society handbook series. I saw copies
available
during the last MSA meeting, but cannot recall who the vendor was. Can
somebody
point me towards a company or individual who may have a copy of this book
available for sale?

Karl Hagglund, Researcher
P&G Food and Beverage Analytical and Microbiology
6071 Center Hill Ave., Box 117
Cincinnati, OH 45224
(513)634-0146




From daemon Tue Mar 20 11:06:41 2001



From: Hooghan, Tejpalkaur K (Tejpal) :      hooghan-at-agere.com
Date: Tue, 20 Mar 2001 12:02:06 -0500
Subject: RE: dental amalgam in SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have done extensive work on amalgams using SEM and TEM. There is no
problem in the vacuum. Mercury is completely consumed/ reacted with alloy
particles and the mercury containing phases are stable to 100C.

Tejpal Kaur Hooghan
Agere Systems
----------
From: White, Woody N. [SMTP:nwwhite-at-mcdermott.com]
Sent: Tuesday, March 20, 2001 8:24 AM
To: 'James M. Ehrman'; 'Microscopy-at-MSA.Microscopy.Com'
Subject: RE: dental amalgam in SEM?


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


I have imaged OLD amalgam without problem. FWIW, A copy of the
image can
be seen on my web site. No experience with "fresh" material.

http://woody.white.home.att.net


Woody
--------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi listers,
}
} Well, I stepped in it this time. I agreed to do some "gee-whiz"
shots
} for my dentist of a tooth with a rather large amalgam filling.
Only
} later
} did it sink in that the mercury might be a problem under
} vacuum and the
} beam. Has anybody out there done this? I'd be looking at the
} thing with
} gold coating an a fairly light beam (say 10 kV), but
} unfortunately, not
} with
} a cold stage. I've checked the archives and found one brave soul
who
} said
} that he's done this on old amalgams. I can't really say how
} old this one
} is, but
} I'm pretty sure it wasn't made yesterday. Call me paranoid,
} but I'd like
} some
} more opinions before I do something really stupid!
}
} Cheers,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}
}
}


From daemon Tue Mar 20 12:31:29 2001



From: jshields-at-cb.uga.edu
Date: Tue, 20 Mar 2001 13:32:14 -0500
Subject: fluorochrome database with multiphoton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I'm having trouble finding a comprehensive source of info on what
wavelengths to use with a multiphoton (Ti:sapphire) laser attached
to a confocal to excite fluorochromes (if there is one yet...).
Specifically, I'm having trouble with the alexa dyes, but a database
of the usual and general dyes would help as well.
I already have a cheat sheet from the confocal manufacturer listing
the usual (FITC, TRITC, DAPI, etc..) and have "discovered" some
not listed (Nile red, aniline blue, alexafluor 594).

Thanks!
john shields
university of georgia
jshields-at-cb.uga.edu


From daemon Tue Mar 20 12:58:00 2001



From: Scott Robinson :      sjrobin-at-itg.uiuc.edu
Date: Tue, 20 Mar 2001 12:56:27 -0600
Subject: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello out there--

I'm trying to prepare cryoTEM specimens of oil emulsions for one of my
users, and it's clear that I don't have plunge-freezing, using ethane,
figured out. I start with a Quantifoil grid, which I've glow-discharged,
held at the edge with a pair of fine forceps. There's an o-ring keeping the
forceps closed. I use a Pipetperson to place 4 microliters of oil emulsion
on one side of the grid. I suspend the forceps/grid over a tiny cup of
liquid ethane, centered within liquid nitrogen, using a guillotine
apparatus with a foot-operated trigger. I blot the grid carefully with a
piece of filter paper. At the instant in which the paper and grid separate,
I trigger the guillotine. (The timing as I've described it differs from
what I've read, but this is how I was shown to do it, and it seems to have
worked -- once or twice. The people who showed me how to do this freeze
virus suspensions, not oil emulsions.)

Now I have my forceps tip/grid in liquid ethane. I detach the forceps from
the guillotine, keeping the grid suspended in the ethane, and then with a
quick movement I immerse the grid in a small cup of liquid nitrogen that's
suspended a few cm away in the same glass dewar. This is where I get a
shallow dome of frozen ethane on the face of my grid. How do I prevent this
from happening? Do I need to blot better, or longer, or blot and then leave
the grid in air briefly before I dunk it? Or is there a trick to getting
the ethane off at this point without damaging the grid?

Scott Robinson











Microscopy Suite
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana IL 61801
217 265-5071 (office); 217 244-6219 (fax)
sjrobin-at-uiuc.edu




From daemon Tue Mar 20 14:07:48 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Tue, 20 Mar 2001 15:09:57 -0500
Subject: Beautiful images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Awhile back I went to an art opening of Stefan Eberhard's photomicrographs.
He's a researcher here at UGA at the Complex Carbohydrate Research Center.
He does
photomicroscopy using polarized light on commonplace chemicals (crystal
images) such as cystine, niacin, vitamin C and my fav image Saccharin. I
thought some of you might enjoy seeing the images. His website -
http://home.att.net/~seberhard
Something to do in your spare time;-)
Beth

Disclaimer: I have no financial interest in this work. Just thought it was nice.

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Tue Mar 20 14:16:50 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 20 Mar 2001 15:12:23 -0500
Subject: Re: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







I'm trying to prepare cryoTEM specimens of oil emulsions for one of my
users, and it's clear that I don't have plunge-freezing, using ethane,
figured out. I start with a Quantifoil grid, which I've glow-discharged,
held at the edge with a pair of fine forceps. There's an o-ring keeping the
forceps closed. I use a Pipetperson to place 4 microliters of oil emulsion
on one side of the grid. I suspend the forceps/grid over a tiny cup of
liquid ethane, centered within liquid nitrogen, using a guillotine
apparatus with a foot-operated trigger. I blot the grid carefully with a
piece of filter paper. At the instant in which the paper and grid separate,
I trigger the guillotine. (The timing as I've described it differs from
what I've read, but this is how I was shown to do it, and it seems to have
worked -- once or twice. The people who showed me how to do this freeze
virus suspensions, not oil emulsions.)

Now I have my forceps tip/grid in liquid ethane. I detach the forceps from
the guillotine, keeping the grid suspended in the ethane, and then with a
quick movement I immerse the grid in a small cup of liquid nitrogen that's
suspended a few cm away in the same glass dewar. This is where I get a
shallow dome of frozen ethane on the face of my grid. How do I prevent this
from happening? Do I need to blot better, or longer, or blot and then leave
the grid in air briefly before I dunk it? Or is there a trick to getting
the ethane off at this point without damaging the grid?



Dear Scott,
Since the ethane is drawn up as you transfer the grid to the LN2, it would
have to be removed during the time it is briefly in the N2 vapor (over the LN2
and ethane) before dunking into the small cup. This would expose your grid to
higher temperatures and, possibly, water vapor, either of which will be far
worse than the ethane. When the cryo-grid is inserted in the EM, the vacuum in
the airlock should be good enough to cause the ethane to evaporate, so, unless
the ethane affects the structure of the oil droplets, you should be better off
just leaving the ethane in place. I would also doubt that there is a good
cryogen which is hydrophyllic enough not to affect the oil, and is, at the same
time, hydrophobic enough not to affect the water. If examination of the grids
shows there to be a problem, you might try to withdraw the grid rapidly from the
ethane--to try to draw up as little as possible--and move the grid quickly
through a volume of dry N2 (the vapor over the LN2 should qualify) to try to
dislodge or evaporate the ethane. I do not think that this procedure will be
easy, and it could well raise the temperature of the specimen to unacceptable
levels if the N2 vapor is not cold enough. Ideally you would want the temp to
be just above the ethane boiling point. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us






From daemon Tue Mar 20 15:21:51 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Wed, 21 Mar 2001 08:16:37 +1100
Subject: Re: Ask-A-Microscopist: GUM MEDIA

Contents Retrieved from Microscopy Listserver Archives
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Could this be gum arabic? It is a mounting medium for permanently mounting
specimens under coverslips, and in another form (perhaps more dilute?) it
can be used as a general adhesive.

}
} Colleagues
}
} I've never heard of "Gum Media" can someone answer this question?
}
} Nestor
}
} ---------------------------------------------------------------------------
}
} Email: JNDKepnerLovers-at-aol.com
} Name: Josh Kepner
}
} Education: 9-12th Grade High School
}
} Location: City, State, Country
}
} Question: We recently bought a home microscopy kit and some "GUM MEDIA" was
} included in the set but was not explained in the manual. What is gum media
} and how would we use it? HELP!?
}
} ---------------------------------------------------------------------------


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Mar 20 16:06:08 2001



From: John Minter :      john.minter-at-kodak.com
Date: Tue, 20 Mar 2001 17:00:00 -0500
Subject: RE: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
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Scott Robinson asked:
..I get a shallow dome of frozen ethane on the face
of my grid. How do I prevent this from happening?

The answer depends upon how much ethane is retained and
where it is located. A small covering of solid ethane
actually serves as an additional frost shield. This will
pump off in the airlock of the microscope. On our Philips
CM-20 we actually insert the holder into the airlock and
pump for a while before dumping the LN2 and inserting the
holder. This will work on a standard airlock with increased
pumping time by the cryo software. Our airlock is a special
turbo-pumped system that presumably gives us a cleaner vacuum.

The problem comes when there is so much ethane film that
it interferes with loading in the holder. When a solid
ethane film splits off the grid, it usually takes the
sample with it. This is very annoying (to say the least).
We have blotted the grid on a dry piece of filter paper
in the cryochamber prior to plunging in the liquid nitrogen.
This works ok if you work fast. It is another opportunity
to get frost contamination on the sample so we only do this
as a last resort...

I'd be interested in hearing what problems you have with
oil emulsions. Our work, along with that of Ishi Talmon,
suggests when you have high concentration of oil phase
in contact with ice (vitreous or otherwise) that radiation
damage (visible as bubbling) is worse than in most aqueous
based systems.

Best Regards,

John Minter
Eastman Kodak Co.
Analytical Technology Division
Rochester, NY 14650-2152
Phone: (716) 722-3407
FAX: (716) 477-9303


From daemon Tue Mar 20 16:50:01 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 20 Mar 2001 16:45:27 -0600
Subject: LR White Resin Removal from Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm passing on a question from a colleague. Does anyone know of a technique
for etching LR White from sections on a slide? I gave him all the info I had
on epoxy removal using ethoxide and so on, but I've been unable to locate
any references specific to LR White.

Thanks.
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Tue Mar 20 17:02:03 2001



From: Chris Combs :      combsc-at-zeus.nhlbi.nih.gov
Date: Tue, 20 Mar 2001 17:58:20 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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----------------------------------------------------------------------------
-------------------------------------------------------------------
Christian A. Combs, Ph.D. office: 301-496-0014
Confocal Core Facility, NHLBI, NIH lab: 301-496-3217
9000 Rockville Pike fax:301-402-2389
Bldg. 10, Rm. B1D-416
Bethesda, MD 20892
----------------------------------------------------------------------------
------------------------------------------------------------------


From daemon Tue Mar 20 18:32:24 2001



From: Rita Monahan-Earley :      rmonahan-at-caregroup.harvard.edu
Date: Tue, 20 Mar 2001 18:30:13 -0600
Subject: Carbon Source- Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Technics sputter coater- Hummer -V. I'd like help finding a
carbon source for it.
Any ideas?
Thank you.




From daemon Tue Mar 20 18:37:10 2001



From: Miriam C. Ritter :      mritter-at-gatan.com
Date: Tue, 20 Mar 2001 18:36:40 -0600
Subject: Gatan SEM Sample Prep School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



SEM Specimen Preparation School
May 10 - 11, 2001


This course has been designed to teach microscopists the preparation of high
quality SEM and Light Microscopy specimens using new broad ion beam
techniques and instrumentation. The two-day practical course intended to
offer end-users an alternative/replacement method to the traditional "wet"
chemical etching technique, used as final step in the sample preparation
process.

Please visit http://www.gatan.com for details.

To register, please e-mail info-at-gatan.com and request a Gatan Schools
Registration Kit.

Miriam Ritter
Gatan, Inc.
925.224.7340




From daemon Tue Mar 20 18:44:32 2001



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Tue, 20 Mar 2001 18:44:00 -0600
Subject: Optical Microscope Available:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Olympus POS for sale. Good monocular PLM student scope. Comes with
Mahogany case, 10x, 20x and 40x stage, Leitz spindle. All offers welcomed.
Lou Solebello microls1297-at-mindspring.com




From daemon Tue Mar 20 18:51:26 2001



From: John C. Gilkey :      jgilkey-at-u.arizona.edu
Date: Tue, 20 Mar 2001 17:50:40 -0700
Subject: Re: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
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} } ...I immerse the grid in a small cup of liquid nitrogen that's
} suspended a few cm away in the same glass dewar.

Scott,
Just put a small piece of filter paper in the cup and drop the
grid on it; the excess ethane will be wicked off by the filter paper.

John


From daemon Tue Mar 20 19:23:27 2001



From: Svetla Stoilova-McPhie :      svetla-at-burnham.org
Date: Tue, 20 Mar 2001 17:18:12 -0800
Subject: Re: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott,
Why don't you try pure grade propane? The freezing procedure is exactly
the same as with the ethane. You will not get frozen ethane, but may
have contamination from comercial propane if you do not find pure one.
svetla


From daemon Wed Mar 21 05:49:02 2001



From: BSIrie-at-aol.com
Date: Wed, 21 Mar 2001 06:42:03 EST
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


thanks


From daemon Wed Mar 21 05:57:59 2001



From: Markus Drechsler :      Markus.Drechsler-at-uni-jena.de
Date: Wed, 21 Mar 2001 12:51:38 +0100
Subject: Re: cryoTEM: excess ethane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott Robinson asked:
.. Do I need to blot better, or longer, or blot and then leave
the grid in air briefly before I dunk it? Or is there a trick to getting
the ethane off at this point without damaging the grid?

You should use a small cryochamber with enough space for the ethane cup,
the specimen holder, some small tools and a piece of filter paper all
located above a boiling liquid nitrogen bath. The evaporating nitrogen
prevents all parts from contamination with ice. After immersing the grid
into the ethane the grid should be blotted on the dry filter paper where
the excess of liquid ethane is drained off. When the specimen holder is
located near by the transfer from liquid ethane to the holder via the
filter paper can be done very fast. In the humidity free atmosphere of the
chamber the intermediate storage in liquid nitrogen can be omitted.

Best regards,
Markus




*******************************************
Dr. Markus Drechsler
Friedrich-Schiller-Universitaet Jena
Institut fuer Pharmazie
Lehrstuhl fuer Pharmazeutische Technologie
Lessingstr.8
D-07743 Jena

Tel: +49 (0) 36 41 / 9-49903
+49 (0) 36 41 / 9-49915 TEM
http://www.uni-jena.de/~b7drma
http://www.mardre.com


*******************************************



From daemon Wed Mar 21 06:57:41 2001



From: Sue Betteridge :      sue-at-rms.org.uk
Date: Wed, 21 Mar 2001 12:51:10 -0000
Subject: Re Finding a book

Contents Retrieved from Microscopy Listserver Archives
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Hi Karl

You can order 'Food Microscopy', and indeed any other Royal Microscopical
Society Handbook, from the publisher, Bios Scientific.

Contact sales-at-bios.co.uk to order directly, or visit www.bios.co.uk or our
website, www.rms.org.uk/handbook.html if you want more information on any of
the RMS handbooks.

Best wishes

Sue
**************************************************************************
Sue Betteridge, Publications Officer, Royal Microscopical Society
37/38 St Clements, Oxford OX4 1AJ, UK. Tel +44 (0)1865 248768, fax +44
(0)1865 791237, email jmicrosc-at-rms.org.uk, website http://www.rms.org.uk
**************************************************************************



From daemon Wed Mar 21 06:58:38 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 21 Mar 2001 06:49:00 -0600
Subject: RE: Carbon Source- Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Rita,

My sugestion would be to either build or buy a carbon evaporator. While it
may not be impossible to sputter carbon with more exotic sputtering devices,
a Hummer V will not work.

Wooody White
------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------.
}
}
} I have a Technics sputter coater- Hummer -V. I'd like help finding a
} carbon source for it.
} Any ideas?
} Thank you.
}
}
}


From daemon Wed Mar 21 07:58:31 2001



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 21 Mar 2001 09:27:20 -0500
Subject: Re: LR White Resin Removal from Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://www.biotech.ufl.edu/icbr/emcl/db/dissolve.html


This is a discussion archived at "Tips & Tricks" There might be more than one, but this is the first I hit with the search.

The rest of the archive can be found at:

www.biotech.ufl.edu/~emcl

Follow the Tips & Tricks link.

I will begin adding material to the site soon. Anything you have found useful in the course of your studies would be welcome. Simply forward it to whittaker.scott-at-nmnh.si.edu. You will of course be given the credit.

Scott Whittaker
SEM Lab Manager
National Museum of Natural History
Smithsonian Institution
10th & Constitution Ave, NW
Washington DC 20560-0104
202-357-1651
{*^-at-)~~~
It was recently discovered that research causes cancer in rats.
~~~(-at-^*}


} } } "Tindall, Randy D." {TindallR-at-missouri.edu} 03/20/01 05:45PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

I'm passing on a question from a colleague. Does anyone know of a technique
for etching LR White from sections on a slide? I gave him all the info I had
on epoxy removal using ethoxide and so on, but I've been unable to locate
any references specific to LR White.

Thanks.
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/







From daemon Wed Mar 21 08:59:22 2001



From: Leonardo Lagoeiro :      lagoeiro-at-degeo.ufop.br
Date: Wed, 21 Mar 2001 11:57:25 -0300
Subject: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there,

I would like to hearing from you opinions about Scanning microscopes.
We have a limited fund to buy a new scanning electron microscope and
we have just received a proposal from a Japanese company named
SHIMADZU. They offered us a scanning electron microscope model
SS-550. I confess that I don't know much about this company and
neither about the quality of its instruments (particularly SEM). I
would appreciate if someone out there could give me an opinion about
this equipment. Right now we can buy a unit without the EDS
analitical system which we are planning to buy later. Then I also
would like to know if we would buy only the image unit first we could
buy later the EDS system (from the same company) separately to
upgrade the same model (SS-550). The vendor said that it is possible,
but sometimes it is hard to trust in sellers especially when you live
in South America.

Thank all of you

Best wishes,


Leonardo
--
---
Leonardo Lagoeiro
Departamento de Geologia
Universidade Federal de Ouro Preto
Ouro Preto, MG, 35400-000
Brazil
E-mai: lagoeiro-at-degeo.ufop.br


From daemon Wed Mar 21 11:32:48 2001



From: Howard Mulhern :      howard.mulhern-at-TCH.Harvard.edu
Date: Wed, 21 Mar 2001 12:27:17 -0500
Subject: Cholesterol Staining

Contents Retrieved from Microscopy Listserver Archives
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I am trying to find a procedure to detect cellular and membrane
cholesterol at the EM level in RBC's. Using the standard EM processing
techniques, cholesterol is dissolved out leaving familiar clefts. Does
anyone out there know of a protocol where I can preserve the cholesterol
at the EM level and still have decent ultrastructure.

Howard Mulhern
Children's Hospital Dept. of Pathology
Surgical Path EM Facility
Boston, 02115, Ma.



From daemon Wed Mar 21 11:32:51 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 21 Mar 2001 11:27:09 -0600
Subject: DLS software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

I'm looking for a simple DLS program, freeware and preferably source code.
This would be "distance least squares" for cleaning up atomic positions (for
example those input by hand from a model), minimizing the mean squared
deviation from expected bond angles and spacings.

I'm particularly interested in code which would do this for silicates
(SiO2), using typical ~1.6 Angstrom Si-O distance and 109 degree Si-O-Si
angle.

I actually found something of this kind, ostensibly free for downloading, at


http://www.kristall.ethz.ch/LFK/software/xrs/

However, I'm consistently refused the ftp connection listed there, and have
gotten no response to queries.

Does anyone know of other sources of such software?

Thanks,

Wharton Sinkler
UOP LLC



From daemon Wed Mar 21 12:27:43 2001



From: Caroline Miller :      camiller-at-creighton.edu
Date: Wed, 21 Mar 2001 12:24:55 -0600
Subject: Aldehyde Removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for a method to remove aldehydes from tissue before
processing? Thanks, Caroline Miller



From daemon Wed Mar 21 13:13:51 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 21 Mar 2001 13:08:28 -0600
Subject: Re: Aldehyde Removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
If you look up protocols for doing PAS staining to show
polysaccharides (in plant and I suppose any tissue), they start with
an aldehyde quench, with either of two chemicals. Years ago we
discovered that for our purposes we don't need that step, and now I
have forgotten the name of the chemicals. The names are something
like DNPH and Dimedone but I can't remember exactly. In any book of
botanical microtechnique that gives the PAS procedure, they should be
there.

Sorry about my flawed memory.

Tobias

}
}
} I'm looking for a method to remove aldehydes from tissue before
} processing? Thanks, Caroline Miller

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Mar 21 14:50:54 2001



From: Wayne England :      england.w-at-bodycote.ca
Date: Wed, 21 Mar 2001 15:44:25 -0500
Subject: Analytical SEM Job Announcement - Toronto

Contents Retrieved from Microscopy Listserver Archives
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Electron Microscopist, Physical Characterization

Bodycote Ortech is seeking an experienced electron microscopist for our
physical characterization group. The successful candidate will provide
hands-on microscopy services to our clients in the healthcare, general
manufacturing and materials sectors. You will characterize a wide variety
of man-made and naturally occurring materials, and investigate materials
breakdown and failure in support of manufacturing processes. You will work
with our clients to define project scope, write quotations, perform
analytical work and prepare project reports.

Your expertise lies in analytical scanning electron microscopy, including
low temperature SEM. You are adept at using light microscopy and image
analysis techniques. You enjoy problem solving and research activities,
manage your time well and are able to work effectively in a team atmosphere.
Ideally you have a graduate degree in chemistry, biology or a related field
and more than three years experience working in an electron microscopy
laboratory.

To join our team, please forward your resume to Human Resources Department,
Bodycote Ortech, 2395 Speakman Drive, Mississauga, Ontario, L5K 1B3, email
bruce.a-at-bodycote.ca
Fax: (905) 823-1446








From daemon Wed Mar 21 15:30:42 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 21 Mar 2001 16:19:46 -0500 (EST)
Subject: Re: Aldehyde Removal

Contents Retrieved from Microscopy Listserver Archives
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Ammonium chloride (50-100 mM) in PBS or glycine (sorry, don't remember
concerntration--probably 1 or 2 % ??) in PBS have been reported. We use
NH4Cl.

Sara Miller

On Wed, 21 Mar 2001, Caroline Miller wrote:

} Date: Wed, 21 Mar 2001 12:24:55 -0600
} From: Caroline Miller {camiller-at-creighton.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Aldehyde Removal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for a method to remove aldehydes from tissue before
} processing? Thanks, Caroline Miller
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Mar 21 15:45:10 2001



From: Jane LaGoy :      JLaGoy-at-bodycote-imt.com
Date: Wed, 21 Mar 2001 16:31:22 -0500
Subject: Re: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
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Hi Leonardo,

I'm afraid that I have never heard of Shimadzu SEM's or EDS's. Moneywise, I
evaluated JEOL, Hitachi, and KLA/Tencor (Amray) SEM's and found that JEOL
was by far the most economical for equivalent systems. (We also purchased
an EDAX EDS system to go with it.)

I have no interest in JEOL or EDAX (other than being a happy customer.)

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
jlagoy-at-bodycote-imt.com





From daemon Wed Mar 21 15:56:13 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 21 Mar 2001 16:51:39 -0500
Subject: Sputter Coater Responses

Contents Retrieved from Microscopy Listserver Archives
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Here are the replies I have received so far on my question about sputter
coaters. They are as I have received them except I have removed the senders
names. Some are obviously from vendors. These are NOT my opinions I am
simply reflecting what I have learned. YMMV.



___________________

Dear Sir,
I saw your posting on the MSA list server and wanted to know if you were
aware of Cressington products. You can view our web site at
www.cressington.com {http://www.cressington.com} . Also I would offer to
come to your lab to demonstrate our 208HR sputter coater. As far as price
being an issue we are always tailoring quotes around tight budgets. Please
feel free to contact me if you would like more information.

___________________

We have a KMC Ion-Tech, which I've been told has been taken over by
South Bay Technologies. This is good. KMC was everything bad in a
company.
If South Bay has redesigned -- extensively -- the ion-coater, then
this might be a good machine. Otherwise, I'd avoid it like the
plague. Even though I generally think good thoughts about South Bay.

When the thing works right, we use it for platinum-coating specimens
for FESEM down to 1 or 2 nm (measured to 0.7 nm, but that really
requires the right specimen). The Pt coat is very fine. I saw grain
structure once at something over 200,000X (might have been 400,000,
this was a while ago), but typically I see no structure.

___________________

We used a sputter coater with a chromium target for awhile, with good
results. You are correct, however, in that the chromium begins oxidizing
immediately and will quickly form an insulating oxide layer. Samples should
be viewed as quickly as possible after coating and stored in the best vacuum
possible if repeated viewings are required. Chromium is not a good way to
go for specimens that need to be kept for repeated viewings.

Our compromise was to purchase a platinum target, which provides somewhat
larger grain size than chromium, but smaller than gold or gold-palladium.
Platinum, of course, does not oxidize. Platinum and other targets are
available at very reasonable cost (relative to the sputter coaters'
manufacturer's prices) from Abe Dayani at Refining Systems, Inc. He can be
reached at P.O. Box 72466, Las Vegas, NV 89170. Phone is (702) 368-0579
and fax is (702) 368-0933. I have dealt with him before and found him to be
very helpful and knowledgeable.

We are using an EMITECH sputter coater currently, but I cannot in good faith
recommend them at this time. The coater works well for the most part, but
the company is recovering from some internal problems and their service is
spotty (to be kind). I think that if or when the company is over its
present difficulties that this will again be a wonderful line of equipment.

___________________


I can't address relative grain size and longevity, nor Cr coats
specifically, but the carbon coater we opted for last year is the Emitech
950C. It also has an easily interchangeable metal head. We felt this turbo
unit was the best value by a considerable stretch. So far we've not done
metal [though may do some Au-Pd fairly soon], only carbon, but the carbon
coats have been very consistent. URL:

http://www.emitech.demon.co.uk/K950.htm

US contact: Linda Dailey {emitech-at-earthlink.net}

If you feed power to your roughing pump through the K950, allowing
automatic control of the rotary by the coater, there is a fuse/circuit issue
we had that is solved by using the auto control for a relay switch.

Best of luck.


___________________


The 681 and the 682 BOTH provide an amorphous coating. The 682 also does
ecthing. The 681 is coating ONLY.

If you want to talk with someone about applications, please call Dick Mitro
at 925-224-7319.

If you don't mind seeing the coating(s), then a cheap $5-$10K coater will
suffice.

___________________

I recently went through this decision process for a HR coater for
our new LEO 1550. I decided on the Cressington 208HR. It can do Cr, but
also Au or Pd or Pt or Au/Pd. It has several nice features such as a
rotary/planetary/tilting stage to allow for a thin 2nm coating even on
rougher samples. My research led me to this coater or one by Emitech. I
think both are good coaters and both were in the $25,000 range. We are
taking delivery of the Cressington in about X weeks. If you are still
looking at that point I can tell you what I think once I get a chance to
use it on several of our samples. Of course I had them coat some samples
for us before I made my decision but lets see how it works in the lab. We
run a wide range of samples, polymer, metal, ceramic. Good luck.


___________________


I saw this posted on the listserver and thought you might have an interest
in our IBS/e Ion Beam
Sputter Deposition and Etching System. It is on the pricey side compared to
a sputter coater
($50-60k), but can deposit very thin, uniform, small grain films using
chromium, iridium, carbon
etc. It has many advantages over magnetron systems.

If it is of interest, I would be happy to send you more detailed
information. Please let me know.


___________________

IMHO!
I know it is not the least expensive choice, but I recently purchaced an SBT
IBSS (Ion Beam Sputter/etch System). It does a fantastic job with Ir. We
have a LEO 1530 (FEG) and routinely image up to } x200,000 with no evidence
of the coating structure. It's durability is fine for us, we have specimens
in us often for several days and up to a week or more, without significant
deterioration in conductivity. Vince Carlino is with them (South Bay) now
and this coater is basically his previous system in a new and improved
system/package. Of course you can use Cr or Pt or virtually any other metal
to "coat", but we have had tremendous success with Ir. It is a very
flexible, durable, and effective instrument for us.


___________________


For DC sputtering the next best option is Pt, better than Au or Pd or
Au/Pd mix.

However Cr is best but has all of the inherent issues that you have
mentioned, We are currently evaluating using Irridium for DC sputtering,
all the advantages of Cr in grain size but none oxidizing.

However very hard to obtaining targets of sufficient purity and very
expensive.

Have a look at our site in the US this shows our equipment and has some
tech information that you may find useful.

www.empdirect.com

The Emitech K575X turbo sputter coater is designed with Cr and FE sem in
mind, the unit may also be used to coat with noble metals as mentioned
above.

Please call or email if you have any questions or would like price
information on any of our products.


___________________

I too am interested in purchasing another sputter coater for use with our
JEOL FEG-SEM. For the last 8 years or so we have been using a Plasma
Sciences CrC-100 coater with a chromium target. Generally the unit has
served us well, however, I have had two nagging concerns with respect to
this coater, 1) its age - it's becoming tempermental of late and 2) Plasma
Sciences is no longer in business, service and parts have been taken over by
Torr International. I don't know where or from whom you were informed
regarding problems associated with chromium. We have not had that
experience.

___________________

Denton desk II did a good job while I was at university of xxxx with just
gold/palladium on a hitachi s-4000 FESEM.

I now have a Cressington Scientific 108 SE automatic but it is so new I have
yet to really put it through the paces. They say it does a wonderful job and
for the price it better. It is also what the FEI applications lab has for
the FEG demonstrations. Those pics were impressive

___________________

Take a look at URL
http://www.2spi.com/catalog/osmi-coat.html

It is now almost impossible to sell a chromium coater in Japan; the Japanese
learned over the past few years that nothing comes even close to what the
osmium coater will do. I know that sounds almost too good to be true but
consider a) the metal layer is completely amorphous, there is absoulutely no
grain size, and of course, with Cr, there is some point where you do start
to see the grain, and b) the metallization has the same inertness as gold or
Pt, so unlike Cr which starts to oxidize almost immediately, the osmium
metal coating lasts almost forever.


___________________


I've used gold-palladium targets with good results in ordinary
sputter coaters. Emitech and Anatech/Hummer are two brands we've had good
luck
with.


___________________


Pertaining to your recent question posted to Microprober list, perhaps you
may want to try Polaron sputter coater SC series at reasonable price, I
have been using few brands of sputter coater for FESEM, I think this is
recommended.

___________________

Would appreciate hearing what you learn about sputter coaters.

Our 15-year-old Au-coater (Denton) has been reliable and user-friendly, but
considering its age, I could be faced with replacing it before too much
longer.

Topographic artifacts produced by the Au-sputter have been of interest
because of the 'nannobacteria' problem. With our little garden-variety
W-filament SEM, a 30 sec coating does not produce detectable artifacts
(below about 50,000x) whereas longer coating times may (See Fig 7, p. 588
in Folk and Lynch, JSR v. 67).

In the FE-SEM, samples coated with our sputter for 45 sec begin to show
potentially troubling textures (artifacts?) around 80,000x.

Thanks for any info you can share!

___________________

I purchased a Cressington 208 HR Sputter Coater for use with our FEG
SEM. It has worked very nicely. I decided that the Cr target was too much
of a hassle for routine prep work, so for the majority of work, I use Au/Pd
and apply a coating of from 1 to 8 nm in thickness, depending on my samples.
The system has been very reliable, pumps down quickly, and releases the
vacuum quickly. The little shield that swings in and out works to protect
the sample from the initial oxidized Cr from the target surface, but when
the oxide layer is sputtered off, you swing the small shield out of the way
to coat the sample with Cr. The rotating, tilting stage allows for good
coverage of the sample with the target material. I know there are other
good brands out there, but the Cressington works very well.














--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"




From daemon Wed Mar 21 16:36:51 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 22 Mar 2001 11:17:01 GMT+1200
Subject: Re: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SHIMADZU is definitely a small player here in the US.

In the 27 yeatrs I have been involved in SEMs, I have never heard of them.

Regards,

Earl

----- Original Message -----
} From: "Leonardo Lagoeiro" {lagoeiro-at-degeo.ufop.br}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 21, 2001 6:57 AM



I didn't know that Shimadzu (who are a large and reputable scientific
equipment manufacturer who afew years ago bought Kratos)) made an
SEM, and a search of their website www.shimadzu.com revealed none.
However, I see that their regional Brasil website lists not only the
SEM 550, but also the EPMA 1600! I remember hearing, years ago, that
they marketed the ARL EPMA as a Shimadzu in (maybe) Asia, but that
may not have been so.

I'd be very interested to hear more about either instrument.

Anybody know anything?

cheers

rtch





}
} Hi there,
}
} I would like to hearing from you opinions about Scanning
} microscopes. We have a limited fund to buy a new scanning electron
} microscope and we have just received a proposal from a Japanese
} company named SHIMADZU. They offered us a scanning electron
} microscope model SS-550. I confess that I don't know much about this
} company and neither about the quality of its instruments
} (particularly SEM). I would appreciate if someone out there could
} give me an opinion about this equipment. Right now we can buy a unit
} without the EDS analitical system which we are planning to buy
} later. Then I also would like to know if we would buy only the image
} unit first we could buy later the EDS system (from the same company)
} separately to upgrade the same model (SS-550). The vendor said that
} it is possible, but sometimes it is hard to trust in sellers
} especially when you live in South America.
}
} Thank all of you
}
} Best wishes,
}
}
} Leonardo
} --
} ---

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Mar 21 17:16:55 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 22 Mar 2001 09:14:34 +1000
Subject: Re: Aldehyde Removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day Caroline,
You need to carry out an "aldehyde blockade" process, This will convert
all aldehyde groups. Including tissue aldehyde groups.
Method: A saturated solution of 2,4-DNP(2,4dinitrophenyl hydrazine) in
15% acetic acid. Time 0.5Hrs.
Wash thoroughly in tap water. Time 0.5Hrs

If you are going on to do a PAS reaction you then begin your oxidation
step with 1% Periodic acid.
Regards
JVN

Caroline Miller wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm looking for a method to remove aldehydes from tissue before
} processing? Thanks, Caroline Miller

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Wed Mar 21 17:28:12 2001



From: Rolf Brandes :      rbrandes-at-novasite.com
Date: Wed, 21 Mar 2001 15:24:37 -0800
Subject: INDUSTRIAL JOB POSITION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Applied Molecular Evolution (AMEV), is an early stage biotech company with
secure financial funding and high growth potential. We focus on novel high
throughput approaches to drug discovery, screening compound libraries
against thousands of variants of G protein coupled receptors using highly
advanced instrumentation.

We are looking for an entry level Scientist in the department of
Fluorescence Imaging. The candidate is expected to have a Ph.D. and be
experienced in fluorescence imaging. Experience with Ca2+-sensitive dyes and
G Protein coupled receptors is a plus.

We are willing to recognize the added value of highly skilled and motivated
individuals. Novasite employees receive excellent benefits and compensation,
including equity participation and generous performance-linked incentives.

Please Reference Job Code: NR0321A
jobs-at-novasite.com
Novasite Pharmaceuticals
3520 Dunhill Street
San Diego CA 92121
Fax: (858) 597-4950



From daemon Wed Mar 21 17:32:08 2001



From: Rolf Brandes :      rbrandes-at-novasite.com
Date: Wed, 21 Mar 2001 15:28:39 -0800
Subject: Recall: INDUSTRIAL JOB POSITION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rolf Brandes would like to recall the message, "INDUSTRIAL JOB POSITION".


From daemon Wed Mar 21 17:33:06 2001



From: Rolf Brandes :      rbrandes-at-novasite.com
Date: Wed, 21 Mar 2001 15:29:41 -0800
Subject: INDUSTRIAL JOB POSITION (corrected)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


San Diego based Novasite Pharmaceuticals, Inc., a subsidiary of Applied
Molecular Evolution (AMEV), is an early stage biotech company with secure
financial funding and high growth potential. We focus on novel high
throughput approaches to drug discovery, screening compound libraries
against thousands of variants of G protein coupled receptors using highly
advanced instrumentation.

We are looking for an entry level Scientist in the department of
Fluorescence Imaging. The candidate is expected to have a Ph.D. and be
experienced in fluorescence imaging. Experience with Ca2+-sensitive dyes and
G Protein coupled receptors is a plus.

We are willing to recognize the added value of highly skilled and motivated
individuals. Novasite employees receive excellent benefits and compensation,
including equity participation and generous performance-linked incentives.

Please Reference Job Code: NR0321A
jobs-at-novasite.com
Novasite Pharmaceuticals
3520 Dunhill Street
San Diego CA 92121
Fax: (858) 597-4950



From daemon Wed Mar 21 17:42:23 2001



From: William P. Sharp :      wsharp-at-asu.edu
Date: Wed, 21 Mar 2001 16:38:25 -0700
Subject: 3-D Reconstruction Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List -

We are preparing to embark on an in-depth project with outside funding that
will require, among other things, 3-D reconstruction of series' of
transmission electron micrographs of sections of biological material. We
don't have a great deal of recent experience in this area and would welcome
(off list if you are a commercial concern) suggestions for the "best" or
most capable, or most flexible or useful 3-D reconstruction software
including what computer platform is required and what configuration is
considered adequate or better for this type of work. In addition, what
digital data collection system currently available would be best for this
type of work? We presently have a Philips CM12S STEM with a slow scan CCD
camera with a 1k x 1k chip that has done good service for an extended
period, but it seems that it might be a bit difficult to use for serial
section recording and image orientation in preparation for 3-D
reconstruction. If something new in a hardware-software package is
available, we would be interested to hear how it works for others, or to
hear directly from vendors off list.

Separately, it is possible that TEM tomography will also be in our future -
It is our understanding that either at least IVEM or an energy filtered TEM
are required to do useful tomography of thicker sections (0.25 - 0.5 micron
thick). What are others in this area of interest using for microscopes,
computers, and software? Has something of a "standard" come about, or are
people still writing their own code and putting systems together from scratch?

Thanks in advance for any and all information and advice.

Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Wed Mar 21 18:24:28 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 21 Mar 2001 18:19:55 -0600
Subject: RE: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do not know this company either, but why do you have
proposal just from one company? Ask other vendors and
compare their proposals. As for EDS it is better to
by system directly from manufacturer and ask them in
advance proposals too - if SEM you choose is not in
their "standard microscopes" list they may charge you
extra for an adapter.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Leonardo Lagoeiro [mailto:lagoeiro-at-degeo.ufop.br]
} Sent: Wednesday, March 21, 2001 8:57 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM inquiry
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi there,
}
} I would like to hearing from you opinions about Scanning microscopes.
} We have a limited fund to buy a new scanning electron microscope and
} we have just received a proposal from a Japanese company named
} SHIMADZU. They offered us a scanning electron microscope model
} SS-550. I confess that I don't know much about this company and
} neither about the quality of its instruments (particularly SEM). I
} would appreciate if someone out there could give me an opinion about
} this equipment. Right now we can buy a unit without the EDS
} analitical system which we are planning to buy later. Then I also
} would like to know if we would buy only the image unit first we could
} buy later the EDS system (from the same company) separately to
} upgrade the same model (SS-550). The vendor said that it is possible,
} but sometimes it is hard to trust in sellers especially when you live
} in South America.
}
} Thank all of you
}
} Best wishes,
}
}
} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br
}


From daemon Wed Mar 21 19:10:47 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 21 Mar 2001 19:59:50 -0500 (EST)
Subject: Re: Aldehyde Removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Caroline didn't say whether she wanted the aldehyde procedure for LM or
EM, but I suspect the 15% acid wouldn't be to good for ultrastructural
studies.

On Thu, 22 Mar 2001, John V Nailon wrote:

} Date: Thu, 22 Mar 2001 09:14:34 +1000
} From: John V Nailon {J.Nailon-at-mailbox.uq.edu.au}
} To: Caroline Miller {camiller-at-creighton.edu}
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Aldehyde Removal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} G'day Caroline,
} You need to carry out an "aldehyde blockade" process, This will convert
} all aldehyde groups. Including tissue aldehyde groups.
} Method: A saturated solution of 2,4-DNP(2,4dinitrophenyl hydrazine) in
} 15% acetic acid. Time 0.5Hrs.
} Wash thoroughly in tap water. Time 0.5Hrs
}
} If you are going on to do a PAS reaction you then begin your oxidation
} step with 1% Periodic acid.
} Regards
} JVN
}
} Caroline Miller wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } I'm looking for a method to remove aldehydes from tissue before
} } processing? Thanks, Caroline Miller
}
} --
} John Nailon
} Operations Manager
} The Centre for Microscopy and Microanlaysis
} The University of Queensland
} St Lucia QLD 4072
} Tel: +61-7-33654214
} Fax: +61-7-33654422
} WWW: http://www.uq.edu.au/nanoworld
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Mar 21 19:24:29 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Thu, 22 Mar 2001 11:21:44 +1000
Subject: Re: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could it be that Shimadzu are now the manufactures/distributors of the
Topcon/Akashi/ISI range of instruments??
Regards
JVN

Ritchie Sims wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I didn't know that Shimadzu (who are a large and reputable scientific
} equipment manufacturer who afew years ago bought Kratos)) made an
} SEM, and a search of their website www.shimadzu.com revealed none.
} However, I see that their regional Brasil website lists not only the
} SEM 550, but also the EPMA 1600! I remember hearing, years ago, that
} they marketed the ARL EPMA as a Shimadzu in (maybe) Asia, but that
} may not have been so.
}
} I'd be very interested to hear more about either instrument.
}
} Anybody know anything?
}
} cheers
}
} rtch
}
} }
} } Hi there,
} }
} } I would like to hearing from you opinions about Scanning
} } microscopes. We have a limited fund to buy a new scanning electron
} } microscope and we have just received a proposal from a Japanese
} } company named SHIMADZU. They offered us a scanning electron
} } microscope model SS-550. I confess that I don't know much about this
} } company and neither about the quality of its instruments
} } (particularly SEM). I would appreciate if someone out there could
} } give me an opinion about this equipment. Right now we can buy a unit
} } without the EDS analitical system which we are planning to buy
} } later. Then I also would like to know if we would buy only the image
} } unit first we could buy later the EDS system (from the same company)
} } separately to upgrade the same model (SS-550). The vendor said that
} } it is possible, but sometimes it is hard to trust in sellers
} } especially when you live in South America.
} }
} } Thank all of you
} }
} } Best wishes,
} }
} }
} } Leonardo
} } --
} } ---
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Thu Mar 22 00:59:10 2001



From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 22 Mar 2001 07:53:51 +0100
Subject: Re: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I was searched Shimadzu web site at http://www.shimadzu.com, but there is no

any information about scanning electron microscope.

Henrik

Ritchie Sims wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I didn't know that Shimadzu (who are a large and reputable scientific
} equipment manufacturer who afew years ago bought Kratos)) made an
} SEM, and a search of their website www.shimadzu.com revealed none.
} However, I see that their regional Brasil website lists not only the
} SEM 550, but also the EPMA 1600! I remember hearing, years ago, that
} they marketed the ARL EPMA as a Shimadzu in (maybe) Asia, but that
} may not have been so.
}
} I'd be very interested to hear more about either instrument.
}
} Anybody know anything?
}
} cheers
}
} rtch
}
} }
} } Hi there,
} }
} } I would like to hearing from you opinions about Scanning
} } microscopes. We have a limited fund to buy a new scanning electron
} } microscope and we have just received a proposal from a Japanese
} } company named SHIMADZU. They offered us a scanning electron
} } microscope model SS-550. I confess that I don't know much about this
} } company and neither about the quality of its instruments
} } (particularly SEM). I would appreciate if someone out there could
} } give me an opinion about this equipment. Right now we can buy a unit
} } without the EDS analitical system which we are planning to buy
} } later. Then I also would like to know if we would buy only the image
} } unit first we could buy later the EDS system (from the same company)
} } separately to upgrade the same model (SS-550). The vendor said that
} } it is possible, but sometimes it is hard to trust in sellers
} } especially when you live in South America.
} }
} } Thank all of you
} }
} } Best wishes,
} }
} }
} } Leonardo
} } --
} } ---
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand

--
Henrik Kaker, Ph.D.
Metal Ravne d.o.o.
SEM-EDS Lab
Koroska cesta 14, 2390 Ravne
Slovenia
Phone: +386 02 82 21 131
Fax: +386 02 82 20 436
http://www.kaker.com




From daemon Thu Mar 22 02:17:08 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 22 Mar 2001 08:12:10 -0000
Subject: Re: Sputter Coater Responses

Contents Retrieved from Microscopy Listserver Archives
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Some questions arising:
1) The use of iridium for sputtering is news to me, and looks
interesting. I would be interested to see a comparison of iridium film
structure with platinum etc. Can a garden variety gold or
gold/palladium unit sputter iridium, or are more exotic conditions
required?

2) Does anyone have experience of the practical and safety issues
arising from osmium coaters?
Is the release of osmium tetroxide vapour to room atmosphere well
controlled? How economical are they to run? Would they be practical
in an environment where there could be several days or even weeks
between coating runs? Presumably there are no safety issues with the
coated specimens if the metal is inert. True?

Chris

snip

IMHO!
I know it is not the least expensive choice, but I recently purchaced
an SBT
IBSS (Ion Beam Sputter/etch System). It does a fantastic job with Ir.
We
have a LEO 1530 (FEG) and routinely image up to } x200,000 with no
evidence
of the coating structure. It's durability is fine for us, we have
specimens
in us often for several days and up to a week or more, without
significant
deterioration in conductivity. Vince Carlino is with them (South Bay)
now
and this coater is basically his previous system in a new and improved
system/package. Of course you can use Cr or Pt or virtually any other
metal
to "coat", but we have had tremendous success with Ir. It is a very
flexible, durable, and effective instrument for us.


___________________


For DC sputtering the next best option is Pt, better than Au or Pd or
Au/Pd mix.

However Cr is best but has all of the inherent issues that you have
mentioned, We are currently evaluating using Irridium for DC
sputtering,
all the advantages of Cr in grain size but none oxidizing.

However very hard to obtaining targets of sufficient purity and very
expensive.

Have a look at our site in the US this shows our equipment and has
some
tech information that you may find useful.

www.empdirect.com

The Emitech K575X turbo sputter coater is designed with Cr and FE sem
in
mind, the unit may also be used to coat with noble metals as mentioned
above.

Please call or email if you have any questions or would like price
information on any of our products.


___________________

I too am interested in purchasing another sputter coater for use with
our
JEOL FEG-SEM. For the last 8 years or so we have been using a Plasma
Sciences CrC-100 coater with a chromium target. Generally the unit has
served us well, however, I have had two nagging concerns with respect
to
this coater, 1) its age - it's becoming tempermental of late and 2)
Plasma
Sciences is no longer in business, service and parts have been taken
over by
Torr International. I don't know where or from whom you were informed
regarding problems associated with chromium. We have not had that
experience.

___________________

Denton desk II did a good job while I was at university of xxxx with
just
gold/palladium on a hitachi s-4000 FESEM.

I now have a Cressington Scientific 108 SE automatic but it is so new
I have
yet to really put it through the paces. They say it does a wonderful
job and
for the price it better. It is also what the FEI applications lab has
for
the FEG demonstrations. Those pics were impressive

___________________

Take a look at URL
http://www.2spi.com/catalog/osmi-coat.html

It is now almost impossible to sell a chromium coater in Japan; the
Japanese
learned over the past few years that nothing comes even close to what
the
osmium coater will do. I know that sounds almost too good to be true
but
consider a) the metal layer is completely amorphous, there is
absoulutely no
grain size, and of course, with Cr, there is some point where you do
start
to see the grain, and b) the metallization has the same inertness as
gold or
Pt, so unlike Cr which starts to oxidize almost immediately, the
osmium
metal coating lasts almost forever.


___________________


I've used gold-palladium targets with good results in ordinary
sputter coaters. Emitech and Anatech/Hummer are two brands we've had
good
luck
with.


___________________


Pertaining to your recent question posted to Microprober list, perhaps
you
may want to try Polaron sputter coater SC series at reasonable price,
I
have been using few brands of sputter coater for FESEM, I think this
is
recommended.

___________________

Would appreciate hearing what you learn about sputter coaters.

Our 15-year-old Au-coater (Denton) has been reliable and
user-friendly, but
considering its age, I could be faced with replacing it before too
much
longer.

Topographic artifacts produced by the Au-sputter have been of interest
because of the 'nannobacteria' problem. With our little garden-variety
W-filament SEM, a 30 sec coating does not produce detectable artifacts
(below about 50,000x) whereas longer coating times may (See Fig 7, p.
588
in Folk and Lynch, JSR v. 67).

In the FE-SEM, samples coated with our sputter for 45 sec begin to
show
potentially troubling textures (artifacts?) around 80,000x.

Thanks for any info you can share!

___________________

I purchased a Cressington 208 HR Sputter Coater for use with our
FEG
SEM. It has worked very nicely. I decided that the Cr target was too
much
of a hassle for routine prep work, so for the majority of work, I use
Au/Pd
and apply a coating of from 1 to 8 nm in thickness, depending on my
samples.
The system has been very reliable, pumps down quickly, and releases
the
vacuum quickly. The little shield that swings in and out works to
protect
the sample from the initial oxidized Cr from the target surface, but
when
the oxide layer is sputtered off, you swing the small shield out of
the way
to coat the sample with Cr. The rotating, tilting stage allows for
good
coverage of the sample with the target material. I know there are
other
good brands out there, but the Cressington works very well.














--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"






From daemon Thu Mar 22 02:32:45 2001



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 22 Mar 2001 03:29:13 -0500
Subject: Carbon Source- Sputter Coater

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Hi Rita,

As is mentioned by other members you are unable to use a carbon target with
your coater but you may possibly be able to obtain a "carbon string" head
and power pack for it?

This package usually contains a new top plate with through terminals, a
power pack (not much different to adding a car battery) and some safety
interconnections. Most sputter coater manufacturers have this type of
accessory.

You are basically using a carbon fibre which you "burn" at high temperature
to evaporate the carbon. The coating is not from a point source (as would
the conventional carbon electrodes used in a high vacuum coater) so it is
better for SEM samples. However the carbon is very soft and easily damaged
even when evaporated under (the desired) best sputter coating vacuum level.

In short it is a quick and easy SEM technique but it does not provide a
coat as efficient as a sputtered metal, or provide a coating that would
allow the production of free floating carbon films here, a high vacuum
coater is essential .

Good luck but talk to the manufacturer.

Steve Chapman
Senior Consultant Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Thu Mar 22 05:07:38 2001



From: paul finnegan :      paulfinnegan-at-ireland.com
Date: Thu, 22 Mar 2001 23:12:50 +0000
Subject: EDS Analysis

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Hi all,

I have undertaken several SEM tests with EDS analysis of
minute "Black Spots" that we are having problems with in the Ultra
High Molecular Weight polyethylene components that we manufacture
at our plant. We thought that identifying the spots would help us
eliminate the problem. To a certain extent it did. i.e. any Fe,Cr&
Ni that was found together is almost certainly stainless steel
splinter.

However, it is the other results that are proving difficult to
identify. Fe is present along with S, Si & Na? Another spot yielded
Si, K ,Na and Ca together. A further example of a spot would be;
Fe,Ca,Si,Ti,K and Na.

Would anyone have any ideas on how to identify what these
particles are?

Any information or help would be gratefully appreciated.

Many thanks to all.

Paul Finnegan


_____________________________________

Get your free E-mail at http://www.ireland.com


From daemon Thu Mar 22 08:25:18 2001



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Thu, 22 Mar 2001 08:14:19 -0600
Subject: EDS Analysis

Contents Retrieved from Microscopy Listserver Archives
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Paul,

It's difficult to ID material without knowing how the spectra were collected
and what elements were dominant. You list Si in the three spots that you
describe. If it was the major foreign element, then you might have some
silicate material.

If available it would be good to try Micro IR to further characterize the
spots.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027




paulfinnegan-at-ireland.com on 03/22/2001 07:27:09 AM
To: Microscopy-at-sparc5.Microscopy.Com
cc:


Hi all,

I have undertaken several SEM tests with EDS analysis of
minute "Black Spots" that we are having problems with in the Ultra
High Molecular Weight polyethylene components that we manufacture
at our plant. We thought that identifying the spots would help us
eliminate the problem. To a certain extent it did. i.e. any Fe,Cr&
Ni that was found together is almost certainly stainless steel
splinter.

However, it is the other results that are proving difficult to
identify. Fe is present along with S, Si & Na? Another spot yielded
Si, K ,Na and Ca together. A further example of a spot would be;
Fe,Ca,Si,Ti,K and Na.

Would anyone have any ideas on how to identify what these
particles are?

Any information or help would be gratefully appreciated.

Many thanks to all.

Paul Finnegan


_____________________________________

Get your free E-mail at http://www.ireland.com






From daemon Thu Mar 22 08:45:04 2001



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Wed, 21 Mar 2001 16:38:25 -0700
Subject: 3-D Reconstruction Software

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Hi Bill,

There exists a homepage of 3-Dimensional Microscopy Labs
(http://3dem.sdsc.edu/) in which are listed various packages for 3D-EM
Image Analysis. Additionally a mailing list exists.

BTW: A nice animation can be found in a Web-Page of the University of
Utrecht (The Netherlands):
http://emsaserv.bio.uu.nl/3dem/ANIMATED_INTRODUCTION/animated_introduction_1.html

I hope this helps a little bit. Of course, you are welcome to contact me
off-line.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Herbststrasse 7
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: www.tvips.com
Email: ingo.daberkow-at-tvips.com







-------- Original Message --------


Dear List -

We are preparing to embark on an in-depth project with outside funding
that
will require, among other things, 3-D reconstruction of series' of
transmission electron micrographs of sections of biological material. We
don't have a great deal of recent experience in this area and would
welcome
(off list if you are a commercial concern) suggestions for the "best" or
most capable, or most flexible or useful 3-D reconstruction software
including what computer platform is required and what configuration is
considered adequate or better for this type of work. In addition, what
digital data collection system currently available would be best for
this
type of work? We presently have a Philips CM12S STEM with a slow scan
CCD
camera with a 1k x 1k chip that has done good service for an extended
period, but it seems that it might be a bit difficult to use for serial
section recording and image orientation in preparation for 3-D
reconstruction. If something new in a hardware-software package is
available, we would be interested to hear how it works for others, or to
hear directly from vendors off list.

Separately, it is possible that TEM tomography will also be in our
future -
It is our understanding that either at least IVEM or an energy filtered
TEM
are required to do useful tomography of thicker sections (0.25 - 0.5
micron
thick). What are others in this area of interest using for microscopes,
computers, and software? Has something of a "standard" come about, or
are
people still writing their own code and putting systems together from
scratch?

Thanks in advance for any and all information and advice.

Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899


From daemon Thu Mar 22 08:54:17 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 22 Mar 2001 09:51:09 -0500
Subject: Re: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
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Leanardo etc.:

Never heard of them either but a quick internet search showed the
following three sites:

1. Shimadzu Scientific Instruments, Inc.
We provide solutions to analytical laboratory science by producing an extensive
range of products, software and unrivaled customer service. In the United States,
Shimadzu

http://www.ssi.shimadzu.com/


2. Shimadzu - Solutions for Science
Shimadzu Scientific Instruments - Solutions for Science since 1875
http://www.shimadzu.com/


3. Shimadzu Solutions for Science
We provide solutions to analytical laboratory science and medical science by
producing an extensive range of products, software and customer service. In
Europe, Shimadzu has a

http://www.sel.shimadzu.com/

====} As well as a specific South American Local site:

http://www.shimadzu.com.br

===================================

And since none of us seem familiar with Shimadzu I thought this site
was interesting . .

4. Shimadzu SUCKS homepage
Shimadzu specializes in lies, denials and cover-ups!

http://shimadzu.wxs.org/

(Now, take it for whatever its worth, but someone did go the effort and
expense of setting up this site, eh?)

================================




On 21 Mar 2001, at 11:57, Leonardo Lagoeiro wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there,
}
} I would like to hearing from you opinions about Scanning microscopes.
} We have a limited fund to buy a new scanning electron microscope and
} we have just received a proposal from a Japanese company named
} SHIMADZU. They offered us a scanning electron microscope model
} SS-550. I confess that I don't know much about this company and
} neither about the quality of its instruments (particularly SEM). I
} would appreciate if someone out there could give me an opinion about
} this equipment. Right now we can buy a unit without the EDS
} analitical system which we are planning to buy later. Then I also
} would like to know if we would buy only the image unit first we could
} buy later the EDS system (from the same company) separately to
} upgrade the same model (SS-550). The vendor said that it is possible,
} but sometimes it is hard to trust in sellers especially when you live
} in South America.
}
} Thank all of you
}
} Best wishes,
}
}
} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."


From daemon Thu Mar 22 08:54:56 2001



From: Marilyn Levy :      mlevy-at-cellbio.wustl.edu
Date: Thu, 22 Mar 2001 08:56:05 -0500
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My e-mail address was correct. Thanks

mlevy-at-cellbio.wustl.edu




From daemon Thu Mar 22 09:33:34 2001



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 22 Mar 2001 07:28:24 -0800
Subject: RE: SEM inquiry

Contents Retrieved from Microscopy Listserver Archives
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Leonardo writes ...

} ...
} we have just received a proposal from a Japanese company named
} SHIMADZU. They offered us a scanning electron microscope model
} SS-550. I confess that I don't know much about this company and
} neither about the quality of its instruments (particularly SEM).
} ...

Any reputable SEM manufacturer should be able to provide you with a
list of facilities with their SEM in use. If not, you should be wary
about being the first.

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu Mar 22 10:36:58 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Thu, 22 Mar 2001 11:35:03 -0500
Subject: Re: Aldehyde Removal

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I use 1% glycine in PBS. Publish data : 0.15 M glycine or lysine.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Sara Miller {saram-at-duke.edu} 03/21 4:19 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Ammonium chloride (50-100 mM) in PBS or glycine (sorry, don't remember
concerntration--probably 1 or 2 % ??) in PBS have been reported. We use
NH4Cl.

Sara Miller

On Wed, 21 Mar 2001, Caroline Miller wrote:

} Date: Wed, 21 Mar 2001 12:24:55 -0600
} From: Caroline Miller {camiller-at-creighton.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Aldehyde Removal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for a method to remove aldehydes from tissue before
} processing? Thanks, Caroline Miller
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265





From daemon Thu Mar 22 12:04:21 2001



From: Valdes, Erica R Dr. SBCCOM :      erica.valdes-at-sbccom.apgea.army.mil
Date: Thu, 22 Mar 2001 12:59:25 -0500
Subject: Need Advice/Info regarding critical point driers and freeze dryin

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In my continuing quest to morph my lab from materials-oriented to
bio-oriented I may finally get a chance to get a critical point drier or
freeze drier. Could someone with more bio experience than I please help me
out with some guidance? Or primary efforts will be intact bacteria and
viruses although we may ultimately get into some minor amounts of tissue
work. If I can only get either CPD or freeze drier which do I go with? is
there any strong justification for both? what accessories are "must haves"?
how much should I budget? and finally is there anything beyond the drier
and accessories that I should be arranging?

I will not object if any suppliers reading choose to privately send a
budgetary quote with the understanding that I am a US Govt. employee and am
in no way authorized to negotiate purchases or commit resources.

Thank-you!
Erica Valdes
US Army SBCCOM
Edgewood Chem Bio Center
APG, MD
410-436-2608



From daemon Thu Mar 22 13:34:12 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 23 Mar 2001 07:34:08 GMT+1200
Subject: Shimadzu

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Yes, that's what I said.

To find them, you have to go to the Brasilian site, where there the
SEM 550, the EPMA 1600, and an AFM are pictured on
www.shimadzu.com.br/analitica/portugues/microscopia.htm

There's a page for the EPMA, seems to say 5 spectrometers
www.shimadzu.com.br/analitica/portugues/microsonda.htm

Does anybody recognise it as a different brand?

Why only in Brazil?

Strange.

cheers

rtch






} Date: Thu, 22 Mar 2001 07:53:51 +0100
} From: Henrik Kaker {Henrik.Kaker-at-guest.arnes.si}
} Reply-to: Henrik.Kaker-at-guest.arnes.si
} Organization: Metal Ravne d.o.o., SEM-EDS Lab
} To: Ritchie Sims {r.sims-at-auckland.ac.nz} ,
} "microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}
} Subject: Re: SEM inquiry

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
} Hello,
}
} I was searched Shimadzu web site at http://www.shimadzu.com, but
} there is no
}
} any information about scanning electron microscope.
}
} Henrik
}
} Ritchie Sims wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } I didn't know that Shimadzu (who are a large and reputable scientific
} } equipment manufacturer who afew years ago bought Kratos)) made an
} } SEM, and a search of their website www.shimadzu.com revealed none.
} } However, I see that their regional Brasil website lists not only the
} } SEM 550, but also the EPMA 1600! I remember hearing, years ago, that
} } they marketed the ARL EPMA as a Shimadzu in (maybe) Asia, but that
} } may not have been so.
} }
} } I'd be very interested to hear more about either instrument.
} }
} } Anybody know anything?
} }
} } cheers
} }
} } rtch
} }
} } }
} } } Hi there,
} } }
} } } I would like to hearing from you opinions about Scanning
} } } microscopes. We have a limited fund to buy a new scanning electron
} } } microscope and we have just received a proposal from a Japanese
} } } company named SHIMADZU. They offered us a scanning electron
} } } microscope model SS-550. I confess that I don't know much about this
} } } company and neither about the quality of its instruments
} } } (particularly SEM). I would appreciate if someone out there could
} } } give me an opinion about this equipment. Right now we can buy a unit
} } } without the EDS analitical system which we are planning to buy
} } } later. Then I also would like to know if we would buy only the image
} } } unit first we could buy later the EDS system (from the same company)
} } } separately to upgrade the same model (SS-550). The vendor said that
} } } it is possible, but sometimes it is hard to trust in sellers
} } } especially when you live in South America.
} } }
} } } Thank all of you
} } }
} } } Best wishes,
} } }
} } }
} } } Leonardo
} } } --
} } } ---
} }
} } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } Department of Geology Fax : 64 9 3737435
} } The University of Auckland email : r.sims-at-auckland.ac.nz
} } Private Bag 92019
} } Auckland
} } New Zealand
}
} --
} Henrik Kaker, Ph.D.
} Metal Ravne d.o.o.
} SEM-EDS Lab
} Koroska cesta 14, 2390 Ravne
} Slovenia
} Phone: +386 02 82 21 131
} Fax: +386 02 82 20 436
} http://www.kaker.com
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Mar 22 13:35:45 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Thu, 22 Mar 2001 12:50:48 -0500
Subject: XRD equipment

Contents Retrieved from Microscopy Listserver Archives
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Hello listers

I've been asked to look into XRD equipment for our lab here, and was
wondering if any of you know who the major players are in this area. We
deal mostly with polymers and polymer fibers, and want to be able to do both
small and wide angle work.

Thanks for the help.
dz

David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Thu Mar 22 14:23:36 2001



From: Bernd Kraus :      info-at-energyloss.com
Date: Thu, 22 Mar 2001 21:18:49 +0100
Subject: TEM,EELS,EFTEM, Workshop SALSA 2002

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Munich, 22 March 2001

Dear Member of the Microscopy/Microanalysis Listserver,

following the very successful workshops at Lake Tahoe, Leukerbad and Port
Ludlow, the next international workshop on electron energy loss spectroscopy
and imaging will be held in Guadeloupe from May 5 - 9, 2002.

The abbreviated title will be

SALSA 2002

which stands for

'Strategies and Advances in Atomic Level Spectroscopy and Analysis'.

We think that this title very nicely summarises the central themes that will
be discussed at the workshop and also suggests the stimulating environment
in which the workshop will be held. You can find further announcements about
the workshop on the new homepage

http://www.energyloss.com/

where you will also find a form to express your interest in SALSA 2002
(} This Meeting } Interested?).

May we kindly ask you to fill out the form with as much information as
possible. This will help us to demonstrate the level of interest in the
meeting in order to attract possible sponsors, including local authorities!
If you fill in your e-mail address on the form, you will automatically be
placed on our email distribution list and informed about future updates.
Your e-mail address will only be used for information directly related to
the workshop and will not be given to any other parties. If you prefer not
to receive any future e-mails, please help us nonetheless by filling out the
form and simply entering 'no' in the e-mail address field.

We hope to see you all at SALSA 2002!

The organisers:

Andrew Bleloch
Christian Colliex
Bernd Kraus
Ondrej Krivanek
Richard Leapman
Jean-Louis Mansot
Joachim Mayer
David A. Muller
Stephen J. Pennycook





From daemon Thu Mar 22 15:48:03 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 22 Mar 2001 16:42:30 -0500
Subject: osmium coating for FESEM samples

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris Jeffree wrote the following:
============================================================
Some questions arising:
1) The use of iridium for sputtering is news to me, and looks interesting. I
would be interested to see a comparison of iridium film structure with
platinum etc. Can a garden variety gold or gold/palladium unit sputter
iridium, or are more exotic conditions required?

2) Does anyone have experience of the practical and safety issues
arising from osmium coaters?
Is the release of osmium tetroxide vapour to room atmosphere well
controlled? How economical are they to run? Would they be practical
in an environment where there could be several days or even weeks
between coating runs? Presumably there are no safety issues with the
coated specimens if the metal is inert. True?
==============================================================
Chris raised two good points, one I can comment about with greater certainty
than the other:

1] When sputtering precious metals with a typical ("garden variety") "SEM
lab coater", with or without a turbo pump, there is a certain physics going
on, and it is basically the same for all of the precious metals. Without
going into a discussion of the nucleation and growth of a layer, the process
is fundamentally the same, be it Au, Pt, alloys of these, or even Ir.

The mention of Ir in a previous posting was not in the context of a
conventional SEM coater, and presumably there could be a different physics
going on so the conclusion when coating is done in that equipment could be
quite different (e.g. a much finer grain for example).

So while Pt in a conventional SEM coater does give a slightly smaller grain
size, there are some trade offs (e.g. longer coating times) and in any case,
it still does not satisfy one with a FESEM seeking to get the most out of
their equipment.

2] We at SPI, in our demo lab have had one of the original OPC-40 Osmium
Plasma Coaters operating for more than one year and then that was upgraded
to the OPC-60 which is described on our website. Yes, the source of the
osmium is osmium tetroxide, in 0.1g ampoules. For those not used to looking
at osmium tetroxide in ampoules, a 1g amount of the material is described as
being "1/3 of a teaspoon". So we are talking about pretty tiny amounts to
begin with (e.g. 10% of 1/3 of a teaspoon). On the rotary vane mechanical
pump pumping out the unit, in addition to the normal oil mist filter, on top
of that is another filter, we call it an "osmium filter", probably a
misnomer but it is designed to be a further filter of any mist. At the top
is a white paper that serves as an indicator paper: the slightest amount of
tetroxide getting out would turn the paper black.

So the first point is that this white paper is still the original paper that
has been in continuous operation for more than two years. Translated this
means that no tetroxide whatsoever is exiting the pumping system. Even
when the unit is vented to atmosphere, there is a procedure where the
chamber is first pumped out, and then the chamber can be opened. But one
does not get a whiff of tetroxide vapor when the chamber is opened. Those
seeing the demos at the shows would attest to that fact.

Of course, at some point there is some tetroxide being pumped out of the
chamber, and if indeed some of the OsO4 did indeed get to the pump oil as
tetroxide, it would be instantly reduced to the dioxide, something fairly
innocuous, which ends up as a colloid in the pump fluid. So other than the
apparent need to change the pump oil a bit more often, that seems to be the
only consequence we have ever seen from the presence of the tetroxide.

With regard to the unit itself, it is completely safety interlocked, we have
done literally hundreds of demonstrations at M&M, PITTCON, and other
meetings and safety is the number 1 issue that comes up. But once anyone
sees such a demonstration, there is agreement that safety no longer is an
issue. It really is impossible for someone to get a whiff of the bad stuff
when the chamber is opened.

Further information on this point would be the following:
a) It is CE tested (passed the tests) for sale in Europe; these tests are
among the most serious in the world in terms of safety.
b) I have been in laboratories in Japan and they run the units out in the
open although if one really was concerned about that, there is no reason why
the unit could not be run in a fume hood.

Since there are well over 100 of the Nippon Laser coaters installed in Japan
alone, I trust that there must be at least a few users of this equipment in
Japan who might also feel qualified to comment on this approach to the
coating of SEM samples. The unique technology embodied in the OPC-60 Plasma
Coater is covered by US Patent #5855682; it makes interesting reading for
one wanting to understand better the operation of this system.

3] The other issues raised had to do with cost and practicality. While the
number of runs one could get depends of course on the thickness of the
coatings being applied, from our own experience, using 0.1g ampoules, we
typically get 20-40 runs per ampoule, so at a current cost of $8.00 per
ampoule, the cost per run is indeed quite low, and in the ball park of cost
one would associate with coating with gold. The capital cost is comparable
to or less than most chromium coaters.

When the system is turned on, including the pump, the pump down is literally
just a few minutes to coating. If the unit has sat unused for several
months, we would expect that, as with any vacuum system, a bit longer for
the pump down would be needed.

For the long term storage of osmium coated SEM samples, as with any delicate
SEM sample, once prepared, we would recommend storing dry, not but not
necessarily oxygen free.

Disclaimer: SPI Supplies is a distributor of the Osmium Plasma Coating
equipment so we have a vested interest in seeing more coaters being sold.
We would be happy to hear what others have to say about this system, pro or
con.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Mar 22 16:24:59 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 22 Mar 2001 17:18:30 -0500 (EST)
Subject: ethanol/propylene oxide ques.

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Dear Netters,

Question up front: I¹d like to know how many labs go straight from 100%
ethanol (no propylene oxide)to epoxy resin (both Spurr and Epon
substitutes, or any other epoxies).

For years, we have deleted the propylene oxide step a.) to decrease the
processing time and b.) to prevent having to deal with this toxic
chemical. This works fine as long as you use dry ethanol, which we did
by keeping drying beads (molecular sieves) in a small (200-500 ml) bottle
of ethanol and oven-drying the beads when this absolute EtOH stock was
depleted. We also made 3 changes of 100% ethanol (10-15 min each for
small blocks, or longer for bigger pieces).

I have other folks in my realm that have routinely used propylene oxide;
understandably, they don¹t want to change because it works. It would be
nice to have one set of rules that always works. What I¹d like to know
from you is how many of you have eliminated the PO, and are there
instances where you must use it? Are there other procedures, such as
using absolutely dry ethanol or extended times, that you follow if you
use only ethanol?

Reply to me directly to save the net from hundreds of answers, and I will
tabulate and send out a summary.

Thanks,
Sara


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Mar 22 16:49:20 2001



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Thu, 22 Mar 2001 17:49:26 -0500
Subject: Re: SEM inquiry

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Hi All,
I worked as a service engineer for Kratos after they became a
subsidiary of Schimadzu. I installed a surface analysis system in Japan at
the same time as a Schimadzu SEM with WDX detector was being installed in
the room opposite. The system looked very well put together. Unfortunately
since the installation engineer and customers spoke no English I have no
specific information about the quality of the SEMs they make, but I can tell
you they are a multibillion dollar corporation with headquarters in Kyoto,
Japan and a significant presence in the US in facilities in Columbia, MD.
Some of their products are only offered in Japan but they have an extensive
range of analytical instruments that they do offer in the US.

Steve Buckingham
Excellatron Solid State LLC
1640 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 617 812 5920



-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-muohio.edu]
Sent: Thursday, March 22, 2001 9:51 AM
To: microscopy-at-sparc5.microscopy.com


Leanardo etc.:

Never heard of them either but a quick internet search showed the
following three sites:

1. Shimadzu Scientific Instruments, Inc.
We provide solutions to analytical laboratory science by producing
an extensive
range of products, software and unrivaled customer service. In the
United States,
Shimadzu

http://www.ssi.shimadzu.com/


2. Shimadzu - Solutions for Science
Shimadzu Scientific Instruments - Solutions for Science since 1875
http://www.shimadzu.com/


3. Shimadzu Solutions for Science
We provide solutions to analytical laboratory science and medical
science by
producing an extensive range of products, software and customer
service. In
Europe, Shimadzu has a

http://www.sel.shimadzu.com/

====} As well as a specific South American Local site:

http://www.shimadzu.com.br

===================================

And since none of us seem familiar with Shimadzu I thought this site

was interesting . .

4. Shimadzu SUCKS homepage
Shimadzu specializes in lies, denials and cover-ups!

http://shimadzu.wxs.org/

(Now, take it for whatever its worth, but someone did go the effort and
expense of setting up this site, eh?)

================================




On 21 Mar 2001, at 11:57, Leonardo Lagoeiro wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there,
}
} I would like to hearing from you opinions about Scanning microscopes.
} We have a limited fund to buy a new scanning electron microscope and
} we have just received a proposal from a Japanese company named
} SHIMADZU. They offered us a scanning electron microscope model
} SS-550. I confess that I don't know much about this company and
} neither about the quality of its instruments (particularly SEM). I
} would appreciate if someone out there could give me an opinion about
} this equipment. Right now we can buy a unit without the EDS
} analitical system which we are planning to buy later. Then I also
} would like to know if we would buy only the image unit first we could
} buy later the EDS system (from the same company) separately to
} upgrade the same model (SS-550). The vendor said that it is possible,
} but sometimes it is hard to trust in sellers especially when you live
} in South America.
}
} Thank all of you
}
} Best wishes,
}
}
} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."



From daemon Thu Mar 22 18:32:15 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 23 Mar 2001 12:31:48 GMT+1200
Subject: correction re Shimadzu url

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Sorry, folks, my Portugese copying skills not too good

Shimadzu EPMA url is

http://www.shimadzu.com.br/analitica/portugues/microssonda.htm

ie two esses in microssonda

Is it an ARL?

rtch





} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} Organization: Dept of Geology, Univ of Auckland
} To: Henrik.Kaker-at-guest.arnes.si, microscopy-at-sparc5.microscopy.com
} Date: Fri, 23 Mar 2001 07:34:08 GMT+1200
} Subject: Shimadzu
} Priority: normal

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
}
} Yes, that's what I said.
}
} To find them, you have to go to the Brasilian site, where there the
} SEM 550, the EPMA 1600, and an AFM are pictured on
} www.shimadzu.com.br/analitica/portugues/microscopia.htm
}
} There's a page for the EPMA, seems to say 5 spectrometers
} www.shimadzu.com.br/analitica/portugues/microsonda.htm
}
} Does anybody recognise it as a different brand?
}
} Why only in Brazil?
}
} Strange.
}
} cheers
}
} rtch
}
}
}
}
}
}
} } Date: Thu, 22 Mar 2001 07:53:51 +0100
} } From: Henrik Kaker {Henrik.Kaker-at-guest.arnes.si}
} } Reply-to: Henrik.Kaker-at-guest.arnes.si
} } Organization: Metal Ravne d.o.o., SEM-EDS Lab
} } To: Ritchie Sims {r.sims-at-auckland.ac.nz} ,
} } "microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}
} } Subject: Re: SEM inquiry
}
} } --------------------------------------------------------------------
} } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} } ---.
} }
} }
} } Hello,
} }
} } I was searched Shimadzu web site at http://www.shimadzu.com, but
} } there is no
} }
} } any information about scanning electron microscope.
} }
} } Henrik
} }
} } Ritchie Sims wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } I didn't know that Shimadzu (who are a large and reputable scientific
} } } equipment manufacturer who afew years ago bought Kratos)) made an
} } } SEM, and a search of their website www.shimadzu.com revealed none.
} } } However, I see that their regional Brasil website lists not only the
} } } SEM 550, but also the EPMA 1600! I remember hearing, years ago, that
} } } they marketed the ARL EPMA as a Shimadzu in (maybe) Asia, but that
} } } may not have been so.
} } }
} } } I'd be very interested to hear more about either instrument.
} } }
} } } Anybody know anything?
} } }
} } } cheers
} } }
} } } rtch
} } }
} } } }
} } } } Hi there,
} } } }
} } } } I would like to hearing from you opinions about Scanning
} } } } microscopes. We have a limited fund to buy a new scanning electron
} } } } microscope and we have just received a proposal from a Japanese
} } } } company named SHIMADZU. They offered us a scanning electron
} } } } microscope model SS-550. I confess that I don't know much about this
} } } } company and neither about the quality of its instruments
} } } } (particularly SEM). I would appreciate if someone out there could
} } } } give me an opinion about this equipment. Right now we can buy a unit
} } } } without the EDS analitical system which we are planning to buy
} } } } later. Then I also would like to know if we would buy only the image
} } } } unit first we could buy later the EDS system (from the same company)
} } } } separately to upgrade the same model (SS-550). The vendor said that
} } } } it is possible, but sometimes it is hard to trust in sellers
} } } } especially when you live in South America.
} } } }
} } } } Thank all of you
} } } }
} } } } Best wishes,
} } } }
} } } }
} } } } Leonardo
} } } } --
} } } } ---
} } }
} } } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } } Department of Geology Fax : 64 9 3737435
} } } The University of Auckland email : r.sims-at-auckland.ac.nz
} } } Private Bag 92019
} } } Auckland
} } } New Zealand
} }
} } --
} } Henrik Kaker, Ph.D.
} } Metal Ravne d.o.o.
} } SEM-EDS Lab
} } Koroska cesta 14, 2390 Ravne
} } Slovenia
} } Phone: +386 02 82 21 131
} } Fax: +386 02 82 20 436
} } http://www.kaker.com
} }
} }
} }
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Mar 22 19:04:32 2001



From: VCRVINCE-at-aol.com
Date: Thu, 22 Mar 2001 20:00:46 EST
Subject: Questions re Sputter Coater Responses

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Chris,

Ir as an ion beam sputtering target began in'92 after a scientist at Lucent
Technology, formerly Bell Labs, was looking at silicon by STM. Before AFM,
STM was the only type of SPM. In STM the tip contacts the specimen for
tunneling. To increase specimen conductivity without introducing artifacts,
Pt was ion beam deposited in an IBS, instrument developed by VCR GROUP now
called IBS/e manufactured by SBT. Different metals were compared to Pt for
minimum film resistivity and smoothness including Cr, Ti, W & Ir.
Surprisingly Ir films prevented specimen surfaces from STM tip damage, were
as conductive as Pt films, sputtered like Pt ie sputter rate & material
distribution were the same and Ir does not oxidize.

After learning Bell Labs results, we hypothesized that if Ir films minimized
STM tip damage, there may be a benefit to delicate specimens from electron
beam interaction in FESEMs. Therefore thin films of Ir & Pt were deposited
on 200 nm polystyrene spheres dispersed on mica. Several specimens with
different metal thickness of Ir & Pt were examined in a Hitachi in-lens
FESEM. It was observed that the PS spheres with Ir films showed less beam
damage than those with Pt films. During separate TEM studies we learned that
5 to 100 angstrom thick Ir films had virtually identical structure as Pt
films.

Further tests were done in Hitachi & JEOL FESEMs of thin Ir films on various
substrates. No Ir structure, grains, could be seen below 200kX. Since '93,
we have recommended Ir targets for the IBS since most FESEM work is carried
out below 200kX. We still recommend Cr or other refractory metal targets
for examination above 200kX.

Vince Carlino
SBT, Inc.



Some questions arising:
1) The use of iridium for sputtering is news to me, and looks interesting. I
would be interested to see a comparison of iridium film structure with
platinum etc. Can a garden variety gold or gold/palladium unit sputter
iridium, or are more exotic conditions required?

2) Does anyone have experience of the practical and safety issues arising
from osmium coaters?
Is the release of osmium tetroxide vapour to room atmosphere well controlled?
How economical are they to run? Would they be practical in an environment
where there could be several days or even weeks between coating runs?
Presumably there are no safety issues with the coated specimens if the metal
is inert. True?

Chris


From daemon Fri Mar 23 02:28:14 2001



From: Simon Watkins :      swatkins+-at-pitt.edu
Date: Fri, 23 Mar 2001 08:18:58 -0500
Subject: Exploding Hg lamps

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vince
thank you for this information.
Iridium suppliers: Johnson Matthey Noble Metals Division
supply it in various forms from facilities in Royston, Cambridge UK
and West Chester, Pennsylvania USA. Sheet of 99.99% purity up to 10"
wide by 0.1 to 5mm thick is available, and discs, wire etc.
http://www.noble.matthey.com/product/detail.asp?id=34
An information sheet with contact addresses is available from the web
site as a 47k .pdf file

Chris


----- Original Message -----
} From: {"VCRVINCE-at-aol.com"-at-sparc5.microscopy.com}
To: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
Cc: {microscopy-at-sparc5.microscopy.com} ; "John F. Mansfield"
{jfmjfm-at-umich.edu}
Sent: Friday, March 23, 2001 1:00 AM


Folks, this is for a colleague of mine. If you have such a document please
mail it to me at your earliest convenience
Simon


I am seeking information appropriate to preparing an SOP to handle the
eventuality that a mercury lamp explodes in one of our fluorescent
microscopes. I am looking for sepcific information about potential
personnel risks associated with the mercury vapor and what, if any,
decontamination procedures are necessary/appropriate for potentially exposed
equipment or personnel. I appreciate that the possibility of an explosion
is remote but, I am required to address this eventuality.

Thank you again for sharing your findings with me.


-------------------------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
3500 Terrace St
University of Pittsburgh
Pittsburgh PA 15261
Tel: 412-648-3051
Fax: 412-648-8330
URL: http://www.cbi.pitt.edu
--------------------------------------------



From daemon Fri Mar 23 08:43:17 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Fri, 23 Mar 2001 08:43:27 -0600
Subject: TEM DAQ >vendors

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I am in the market for a digital camera upgrade to my LaB6 2010. At
the risk of receiving more information than I want to know about,
venders are welcome to contact me off line to bring me up to date on the
technology. Invitations to bid are expected to follow a review of the
technology.


Thanks,
Bruce Brinson
Optical Analyst
Rice University



From daemon Fri Mar 23 10:23:16 2001



From: =?iso-8859-1?Q?Pog=E1ny?= Lajos :      pogany-at-power.szfki.kfki.hu
Date: Fri, 23 Mar 2001 17:18:40 +0100
Subject: SEM digitalizer

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Dear colleguages,
I'm seeking a controller for a JSM840 whicsh is reliable and can control
the SEM beam in X-Y direction at least with 4096 pixel resolution. And it
would be nice if the stage and the controller would be optically coupled
to the computer. I saw such a controller in some older letter, but I don't
remember where?
I thank any kind of help
LAjos Pogany
dr. Pogány Lajos
Senior Research Fellow,
Metals Research Department,
Research Institute for Solid State Physics and Optics,
Hungarian Academy of Sciences
Office Address: H-1121 Budapest, Konkoly-Thege ut 29-33
Letters: H-1525 Budapest, P.O.B. 49, Hungary
Phone: (00)-36-1-392-2222/17-25; Fax: (00)-36-1-392-2215
e-mail:pogany-at-szfki.hu



From daemon Fri Mar 23 11:00:04 2001



From: Antun Tonejc :      atonejc-at-phy.hr
Date: Fri, 23 Mar 2001 18:00:21 +0100
Subject: Meeting Announcement

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Announcement and Call for Papers
TENTH CROATIAN-SLOVENIAN CRYSTALLOGRAPHIC MEETING
Lovran, Croatia, June 21-24, 2001

The Meeting is open for the contribution dealing with all aspects of
crystallography and its
applications (Chemical Crystallography, Industrial Crystallography,
Biological Crystallography,
Physical Crystallography, Applied Crystallography and Mineralogy) and
its techniques (XRD,
TEM, SEM, STM)

please visit the web site

http://www.chem.pmf.hr/~hkz/lovran01/

Prof. A. Tonejc
Department of Physics
Faculty of Science
Zagreb
Croatia





From daemon Fri Mar 23 11:10:56 2001



From: RCHIOVETTI-at-aol.com
Date: Fri, 23 Mar 2001 12:06:44 EST
Subject: Re: Exploding Hg lamps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 03/23/2001 6:26:00 AM US Mountain Standard Time,
swatkins+-at-pitt.edu writes:

{ { I am seeking information appropriate to preparing an SOP to handle the
eventuality that a mercury lamp explodes in one of our fluorescent
microscopes. I am looking for sepcific information about potential
personnel risks associated with the mercury vapor and what, if any,
decontamination procedures are necessary/appropriate for potentially exposed
equipment or personnel. I appreciate that the possibility of an explosion
is remote but, I am required to address this eventuality.

Thank you again for sharing your findings with me.

} }

Simon,

I know the manufacturers of the bulbs have this info. I have seen the
precautionary statements on the flyers which accompany Osram bulbs, and Ushio
probably has a similar document. If you don't hear from someone who already
has this info, you could probably get it from Osram.

In a nutshell, Osram recommends that all personnel immediately leave the area
for at least 30 minutes to avoid inhaling mercury vapor, and then any mercury
residue, debris, envelope shards, etc. should be cleaned up with a mercury
cleanup kit, and the debris properly disposed of as biohazardous material
with mercury clearly identified as the hazard. Alternatively, if you have a
hazmat team, they could be called in to do the cleanup, I suppose.

I hope this helps.

Cheers,

Bob Chiovetti
GTI Microsystems


From daemon Fri Mar 23 12:03:59 2001



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Fri, 23 Mar 2001 17:59:54 -0000
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I am preparing a sample for TEM. The sample is GaN on sapphire substrate. I
am supposed to make a sample in plane-view. Its needless to say how hard
sapphire is. Grinding it down to 150microns was itself a tough job. Now I
have put it on the dimpler, and using a diamond slurry to grind it. Its been
10 hrs and I have been successful in taking off only 70 microns. Does anyone
have any bright ideas to speed up this process?

Praveena bhaskara
Department of Chemical Engineering.
University of Massachusettes, Lowell
Lowell
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Fri Mar 23 12:34:44 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 23 Mar 2001 13:38:17 -0500
Subject: cryo of bone marrow (LM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

One of my clients needs to do immunocytochemistry on mouse bone
marrow, and needs to use frozen sections. I haven't got a clue where
to begin (she will harvest the bone marrow). Any ideas?

Thanks as always,
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Mar 23 12:43:16 2001



From: paul finnegan :      paulfinnegan-at-ireland.com
Date: Fri, 23 Mar 2001 18:43:18 +0000
Subject: Thank you

Contents Retrieved from Microscopy Listserver Archives
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Hi to ye all!

I'd just like to say "Thank You" to all of you who replied to my
question on the EDS analysis of the contamiants. you answers proved
to be most helpful.

Once again, thank you

Kind regards

Paul

_____________________________________

Get your free E-mail at http://www.ireland.com


From daemon Fri Mar 23 12:45:36 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Fri, 23 Mar 2001 11:49:42 -0500
Subject: Thanks for the response to my XRD question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks all who responded to my question about XRD equipment.

This list is great for the knowledge and helpful people.
Hopefully I'll be able to answer someone questions like you've all answered
mine.

dz

David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Fri Mar 23 12:50:56 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 23 Mar 2001 13:47:46 -0500
Subject: RE: TEM sample preparation for GaN on sapphire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yep!
The small angle cleavage angle works well for GaN on sapphire. I prepared about 8 samples in about 2 hours while teaching the technique to students and staff at the Univ. of Illinois. We only looked at three samples. Two were very very good. I will send you an image off-list. You can obtain information about South Bay Technology's Micro-Cleave kit at their web site, http://www.southbaytech.com

For grinding down the samples, use a 30 or 45 um diamond lapping film with a hand grinder. (I used 30um.) Works well.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Praveena Bhaskara [mailto:bubbyp-at-hotmail.com]
} Sent: Friday, March 23, 2001 1:00 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM sample preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi!
} I am preparing a sample for TEM. The sample is GaN on
} sapphire substrate. I
} am supposed to make a sample in plane-view. Its needless to
} say how hard
} sapphire is. Grinding it down to 150microns was itself a
} tough job. Now I
} have put it on the dimpler, and using a diamond slurry to
} grind it. Its been
} 10 hrs and I have been successful in taking off only 70
} microns. Does anyone
} have any bright ideas to speed up this process?
}
} Praveena bhaskara
} Department of Chemical Engineering.
} University of Massachusettes, Lowell
} Lowell
} ______________________________________________________________
} ___________
} Get Your Private, Free E-mail from MSN Hotmail at
http://www.hotmail.com.



From daemon Fri Mar 23 12:53:33 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 23 Mar 2001 11:40:37 -0800
Subject: TEM sample preparation- GaN on Sapphire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Praveena:
}
} We have an application note on preparing GaN on Sapphire using our
} Tripod Polisher. You can download it from our webiste at
} www.southbaytech.com. Go to Applications Support then click on
} Applications Notes.
}
} I hope this helps.
}
} David
}
} } -----------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hi!
} } I am preparing a sample for TEM. The sample is GaN on sapphire substrate. I
} } am supposed to make a sample in plane-view. Its needless to say how hard
} } sapphire is. Grinding it down to 150microns was itself a tough job. Now I
} } have put it on the dimpler, and using a diamond slurry to grind it. Its been
} } 10 hrs and I have been successful in taking off only 70 microns. Does anyone
} } have any bright ideas to speed up this process?
} }
} } Praveena bhaskara
} } Department of Chemical Engineering.
} } University of Massachusettes, Lowell
} } Lowell
} } _________________________________________________________________________
}
} --
} David Henriks TEL: +1-949-492-2600
} South Bay Technology, Inc. FAX: +1-949-492-1499
} 1120 Via Callejon
} San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
}
} *** www.southbaytech.com ***






From daemon Fri Mar 23 13:31:32 2001



From: Sara Miller :      saram-at-duke.edu
Date: Fri, 23 Mar 2001 12:06:44 EST
Subject: Re: Exploding Hg lamps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This was a good answer.

Hg clean up kits are available from Lab Safety Supply, Box 1368,
Janesville, WI 53547 (800 356 0783).


I have no commercial interest in this company. That's just where we got
our kit. Fortunately, we haven't had to use it.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265

---------- Forwarded message ----------


In a message dated 03/23/2001 6:26:00 AM US Mountain Standard Time,
swatkins+-at-pitt.edu writes:

{ { I am seeking information appropriate to preparing an SOP to handle the
eventuality that a mercury lamp explodes in one of our fluorescent
microscopes. I am looking for sepcific information about potential
personnel risks associated with the mercury vapor and what, if any,
decontamination procedures are necessary/appropriate for potentially exposed
equipment or personnel. I appreciate that the possibility of an explosion
is remote but, I am required to address this eventuality.

Thank you again for sharing your findings with me.

} }

Simon,

I know the manufacturers of the bulbs have this info. I have seen the
precautionary statements on the flyers which accompany Osram bulbs, and Ushio
probably has a similar document. If you don't hear from someone who already
has this info, you could probably get it from Osram.

In a nutshell, Osram recommends that all personnel immediately leave the area
for at least 30 minutes to avoid inhaling mercury vapor, and then any mercury
residue, debris, envelope shards, etc. should be cleaned up with a mercury
cleanup kit, and the debris properly disposed of as biohazardous material
with mercury clearly identified as the hazard. Alternatively, if you have a
hazmat team, they could be called in to do the cleanup, I suppose.

I hope this helps.

Cheers,

Bob Chiovetti
GTI Microsystems




From daemon Fri Mar 23 13:35:42 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 23 Mar 2001 13:42:51 -0600
Subject: EM & DiMethFA?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yo MICROlisters,

I'm in another lab now, and in the hood here I found about 50 ml of DMF (or
DMFA) - see below - and wondering what EMer's might use it for. Here's what
the Merck Index says about it:

---------------------------
from the Merck Index:

DMF = N,N - Dimethylformamide (also abbreviated DMFA); C3H7NO
Colorless to very slightly yellow liquid. Faint amine odor.

Human toxicity: Vapor harmful. Irritating to skin, eyes and mucous
membranes. Liver injury has been produced in experimental animals by
prolonged inhalation of 100 ppm.

Use: Solvent for liquids & gases. In the synthesis of organic compounds.
Solvent for Orlon and similar polyacrylic fibers. Wherever a solvent with
a slow rate of evaporation is required. Has been termed the universal
organic solvent.
----------------------------

Any thoughts will be appreciated, thanks.


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Mar 23 15:57:32 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Fri, 23 Mar 2001 13:18:42 -0500
Subject: TO PO OR NOT TO PO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the person who was wondering weather or not P.O. was needed after
dehydration and before embedding in Spurrs:

For the past 15 years I have been successfully embedding in Spurrs by using
graded steps of acetone and going from my third change in 100% acetone to
one third Spurrs and two thirds 100% acetone with out the use of P.O.. Of
course then I follow that with a 1:1 mixture and then a 2:3 mixture and then
a 100% Spurrs before embedding in 100% Spurrs at 65 degrees Celsius for 12
hours or more. Best of luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Fri Mar 23 16:01:40 2001



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 23 Mar 2001 13:56:19 -0800
Subject: Amray FE SEM Contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I have been receiving conflicting reports concerning Amray service on the FE
SEMs.
The "official" word has been that Amray will continue to support the FESEMs
"for some time" but I have had several customers call me & request service
for their FESEMs as Amray will not offer a contract for the next year.

Has anyone had a similar experience or give me a striaght answer on this
subject?


Thank You,

Earl Weltmer



From daemon Fri Mar 23 16:21:00 2001



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 23 Mar 2001 16:14:00 -0600
Subject: Lead Aspartate

Contents Retrieved from Microscopy Listserver Archives
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I was wondering if anyone has had any experience with lead aspartate "en
Block" staining. I have tried "en block" staining before with disappointing
results, but I have never tried it with lead asparate. The catalog
describes it such that with Lead Aspartate you can then skip the staining
step after sectioning.



From daemon Fri Mar 23 16:23:49 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 23 Mar 2001 14:24:17 -0800
Subject: Re: SEM digitalizer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soft Imaging's ADDA is optically coupled between controlling
computer and physical electrical interface to SEM.

http://www.soft-imaging.com

gary g.


At 08:18 AM 3/23/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 23 17:53:01 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 23 Mar 2001 15:48:36 -0800 (PST)
Subject: ? how to make lacey carbon films?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everyone,
I want to make my own lacey carbon films for cryo-tem and was wondering if
anyone had some good methods, experiences, insights, or references?

I found references for lacey formvar, pure carbon films, and a few
others... But nothing explicitely for lacey carbon.

All comments greatly appreciated, Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Mar 23 18:03:40 2001



From: dfvillamar-at-acuabiotec.com
Date: Fri, 23 Mar 2001 18:02:56 -0600
Subject: Ask-A-Microscopist: SEM of samples of marine zooplankton

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Email: dfvillamar-at-acuabiotec.com
Name: Daniel F. Villamar

Organization: AcuaBiotec LLC

Education: Graduate College

Location: Damascus, MD USA

Question: We are interested in SEM of samples of marine zooplankton, i.e.,
larval and postlarval shrimp. We would like to have a ventral view of the
mouth parts and a view of the inside surface of the gut wall. The latter
would require sectioning of the specimens along the longitudinal axis and
scanning the inside surface starting at the mouth and working down through
the foregut, midgut and hindgut of the specimens.

1. Does enyone provide such a service?
2. How should we fix and preserve the specimens for best result.


---------------------------------------------------------------------------




From daemon Fri Mar 23 20:05:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 23 Mar 2001 21:00:30 -0500
Subject: RE: ? how to make lacey carbon films?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If I remember correctly, you make holey carbon films by first making your holey formvar (or other plastic), coating this film with a thin layer of carbon to make it continuous, and then dissolving the support plastic with a suitable solvent. This is off the top of my head. A few years ago, Mr. Pella suggested some literature to me on how to do it.

It's easier and more cost-effective to buy them from any of the EM supply houses!


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
} Sent: Friday, March 23, 2001 6:49 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: ? how to make lacey carbon films?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello Everyone,
} I want to make my own lacey carbon films for cryo-tem and was
} wondering if
} anyone had some good methods, experiences, insights, or references?
}
} I found references for lacey formvar, pure carbon films, and a few
} others... But nothing explicitely for lacey carbon.
}
} All comments greatly appreciated, Thanks.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
} \/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak
Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085
} Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}


From daemon Fri Mar 23 20:31:27 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 23 Mar 2001 18:32:23 -0800
Subject: Re: Amray FE SEM Contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The word I have received so far is that later model FESEMs will be
covered under contracts. The distinction of "later model" is
a perhaps nebulous term. The 1800 series instruments
will probably not qualify. My 1910FESEM is based on 1800
electronics but uses the 305FE gun--and so far, is covered
under contract. I am good for the rest of CY 2001. If Amray
KLA stopped supporting FESEMs, something major would have
to occur to keep the guns (at a minimum) available to established
systems. The guns seem to last about 2.5-4 years. During
that time, they are rock solid.

It seems to me that the more that a microscopist can do
in regards to maintenance is better in the near term. This
is despite a maintenance contract. If simple things go
wrong and you can fix them, doing this will keep your
system below the cost accountant's radar screen. The key
for having the contract is if something really major goes
wrong. An FE gun under contract is about $2K. Otherwise,
it is at least $18K. How about a turbo pump? No cost
under contract. Otherwise???

what about changing final apertures? I have not done a good
job of doing this. But now I know more about how to do it
correctly. And cleaning the holder is not a trivial process,
nor one to be ignored.

gary g.


At 01:56 PM 3/23/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Mar 24 01:23:04 2001



From: Brian Oates :      brian.oates-at-ubc.ca
Date: Fri, 23 Mar 2001 23:14:18 -0800
Subject: Looking for TEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I am looking for TEM images for two reasons: 1. to become part of a
digital image database for cell biology courses here at the
University of British Columbia; 2. to be part of an image database
used on CDs accompanying the new edition of a popular cell biology
textbook.

I am looking for high quality images of a variety of cell types.
These images can be either digitized or the original negatives. If
they are digitized then they must be scanned-in to standards set by
ourselves and the publisher. If the images are in the form of the
original negatives we can arrange to have them sent here, scanned-in
and returned.

In return contributors to the UBC collection can have access to other
images in the collection and those who's images are used by the
publisher will receive $50/US per image chosen. The publisher does
not want to hold the copyright on the images used, rather they have
proposed a generous licence agreement.

Interested parties can contact me (Brian Oates) at:

brian.oates-at-ubc.ca
--
Dr Brian Oates
Department of Botany
University of British Columbia
Vancouver, B. C. V6T 1Z4
604.822.4630
brian.oates-at-ubc.ca


From daemon Sat Mar 24 01:46:27 2001



From: huziz-at-slomusic.net
Date: Fri, 23 Mar 2001 20:33:00 -0800
Subject: Need an inexpensive safe way to cure snoring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{HTML}
{BODY bgColor=3D#C0C0C0}

{FONT face=3D"MS Sans Serif"}
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Is {/FONT}
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{FONT color=3D"#0000A0"} keeping you up all night? {/FONT}
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{FONT color=3D"#0000A0"} Snoring also causes sleep disturbances that lead =
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Individuals who snore have a 90% chance of daytime fatigue. Nine out of ev=
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Accumulation of secretion in the back of the throat and {BR}
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Heavy snoring is often associated with apnea, or the {/FONT}
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{FONT color=3D"#800040"} {B} The hidden dangers of snoring? {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {B} {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {UL} {LI} Snoring could increase the chances of a h=
eart attack if not treated {BR}
{LI} Hypertension and increased anxiety are associated with snoring {BR}
{LI} Snoring causes sleep apnea a serious treatable medical condition {BR}
{/LI} {/LI} {/LI} {/UL} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#800040"} {B} Snoring decreases sexual performance {/B} {/FONT=
}
{FONT color=3D"#800040"} {/FONT}
{FONT color=3D"#0000A0"} by half, due to lack of oxygen to the brain which=
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{BR}
Most people who snore have a {/FONT}
{FONT color=3D"#800040"} {B} shortage of oxygen {/B} {/FONT}
{FONT color=3D"#0000A0"} for a significant portion of that person's life.=
In recent studies, daytime fatigue and cardiovascular disorder are relate=
d to snoring. In a few cases, heavy snoring is a sign of potentially life =
-threatening problem. Sleep apnea in which breathing stops for a second or=
even minutes at a time and finally resumes with snorting and tossing arou=
nd. This pattern may be repeated hundreds of times all night long. The res=
ult is chronic oxygen shortage, which leads to abnormal heart rhythms, hig=
h blood pressure, and heart strain. {BR}
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{FONT color=3D"#800040"} {B} How the Snore Eliminator works! {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} Snore Eliminator offers a unique break through wi=
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rn eliminates the snoring for hours at a time. It also {/FONT}
{FONT color=3D"#800040"} {B} reduces the noise {/B} {/FONT}
{FONT color=3D"#800040"} made by breathing through the mouth while sleepi=
ng {/FONT}
{FONT color=3D"#0000A0"} . By simply spraying the back of the throat, tong=
ue and uvula with our special formula, manufactured using our patented bre=
ak through process, the soft tissue is coated. This allows the {/FONT}
{FONT color=3D"#0000A0"} {B} {/B} {/FONT}
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{FONT color=3D"#0000A0"} thus a reduced amount of snoring. In most cases =
snoring is eliminated. Snore Eliminator coats the throat area with a uniqu=
e patented process of all natural lubricants. {BR}
{BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} TESTIMONIALS from several of our satisfied cus=
tomers: {BR}
{BR}
{/B} {/FONT}
{FONT face=3D"Times New Roman"}
{FONT color=3D"#FF0000"} {B} Lorenzo writes... {BR}
{BR}
I am having a good nights sleep, finally. I used to wake myself up during=
the night which was a real bummer as I would not always go back to sleep.=
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}
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Glen writes... {BR}
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In February 1995, I was diagnosed with severe sleep apnea. A CPAP was the=
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I heard your advertisement and ordered a bottle of Snore Eliminator and m=
y sleep improved immediately. I continue to use it and my sleep has been =
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{/B} {/FONT}
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#800040"} If you have a snoring problem what can you do? {/F=
ONT}
{FONT color=3D"#0000A0"} Who would you turn to? Look no further, the ans=
wer to your snoring problems are over. Let our patented {/FONT}
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It is estimated that 90 million Americans, over the age of 18,suffer from =
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{BR}
{/FONT}
{FONT color=3D"#800000"} {B} How to Use Snore Eliminator: {/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} Open your mouth {BR}
Hold the 4oz bottle about four inches from your open mouth {BR}
Spray one or two application onto the back of the throat. {/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
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Snore Eliminator is safe to use nightly because of its' natural ingredient=
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Please note as with any health-related product, it is recommended that you=
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{FONT face=3D"Times New Roman"} {BR}
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e offer a 15-day money back guarantee. Merely return the unused portion o=
f Snore Eliminator within 15 days and we will refund your purchase price w=
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{FONT color=3D"#800040"} {B} $19.49 plus $3.95 {/B} {/FONT}
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o offer you Snore Eliminator at this low price as long as you purchase fro=
m us in the future. To take advantage of this savings you must order with=
in the next 10 days. {BR}
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Here's How to order {BR}
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Email address:_______________________________________ {BR}
(if there is a problem with your order this is where we will notify you) {B=
R}
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To: {BR}
{BR}
John Taylor {BR}
CEO {BR}
Internet Information Services, Snore Eliminator Division {BR}
PO Box 21442 {BR}
Billings, MT 59104 {BR}
{BR}
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{/FONT}
{FONT color=3D"#0000A0"} {B} {BR}
To Fax us your Order (Fill out and fax us every page which follows): {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} MAKE YOUR CHECK PAYABLE TO {/B} {/FONT}
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Division, tape your check to the following form and Fax to {/FONT}
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{FONT color=3D"#0000A0"} . If this line is busy, please try faxing to {BR=
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ll receive an 8 to 12 week supply of Snore Eliminator for only $38.98, inc=
luding shipping and handling. This is a 17% savings. {/FONT}
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(if there is a problem with your order this is where we will notify you) {B=
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{FONT color=3D"#0000A0"} . If this line is busy, please try faxing to {/FO=
NT}
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{FONT color=3D"#0000A0"} Thank you for your business, {BR}
{BR}
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Internet Info Service, Snore Eliminator Division {BR}
PO Box 21442 {BR}
Billings, MT 59104 {BR}
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From daemon Sat Mar 24 13:36:47 2001



From: m.andersson-at-t-online.de (Maike Andersson)
Date: Sat, 24 Mar 2001 16:58:55 +0100
Subject: LM-staining lignin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

Can anyone suggest a stain for suberin/cutin
for LR-white embedded plant tissues?

Thanks in advance
Maike Andersson



From daemon Sat Mar 24 14:41:53 2001



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Sat, 24 Mar 2001 21:37:29 +0100
Subject: Re: LR White Resin Removal from Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Randy,
try 100% acetone,5 min, RT, use coated slides (e.g. silicate, poly-l-lysine)

Michael

Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I

} Hi,
}
} I'm passing on a question from a colleague. Does anyone know of a technique
} for etching LR White from sections on a slide? I gave him all the info I had
} on epoxy removal using ethoxide and so on, but I've been unable to locate
} any references specific to LR White.
}
} Thanks.
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}

______________________________________________________________________________
Die Fachpresse ist sich einig: WEB.DE 20mal Testsieger! Kostenlos E-Mail,
Fax, SMS, Verschlüsselung, POP3, WAP....testen Sie uns! http://freemail.web.de



From daemon Sat Mar 24 16:03:29 2001



From: Michael McInerney :      michael.mcinerney-at-rose-hulman.edu
Date: Sat, 24 Mar 2001 16:59:57 -0500
Subject: SEM usage costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I am negotiating with my college to purchase a block of time on their
new variable pressure SEM (Hitachi S-3000N with EDX). We have agreed
to abide by the 'average' cost per hour charged by universities and
colleges. I seem to remember that there was a discussion along these
lines some time ago but I cannot find it in the archives. If you pay
per-hour fees for SEM usage I would be much obliged if you would let me
know what they are.

Thanks

Michael


Michael McInerney
Rose-Hulman Institute, Terre Haute, IN.



From daemon Sat Mar 24 17:36:52 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 24 Mar 2001 17:31:56 -0600
Subject: RE: Amray FE SEM Contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amray customers, as well as the rest of us, are scrambling to figure out a
lot of things. I've had loyal Amray customers running to me because
they've had their service dropped, they've had implied threats that their
service would be dropped in the near term and because they are just sick
and nervous of the way the whole thing was handled by the manufacturer. I
am quite sure that there are many who are just plain confused over whether
they have been dropped or not.

On the other hand, a new customer of mine had to field a question from an
Amray rep over whether they were renewing a contract on a 3300 FESEM. When
told they were not, the rep apparently went over the line about the
'impossibility' of getting other service and the 'proprietary' nature of
their service.

I think that, having punched a hole in their own boat, Amray is now
surprised that they are taking on water.

There are a lot of long-time, dedicated people at Amray who feel betrayed
by their own company now. I know, having been through a very similar
business turn with ETEC when they were bought out by Perkin-Elmer and their
SEM business was left to suffer an ignoble death. It's hard to believe
that anyone could possibly have made such decisions without having a long
term desire to shutdown the business.

The only question that seems to remain is - what business is being shut
down? Amray has always been a major player in the semi-conductor field.
These days, the FESEM is the most attractive portion of that business. My
guess would be that they have chosen to still pursue the semi manufacturers
with FESEMs but close down their general purpose SEM business. In the mean
time, they want to still provide the lucrative service contracts on the
general purpose FESEMs out there. Cash is king, and the cash in their
operation is in the selling and service of FESEMs, primarily to those
customers who are willing and able to pay a premium for these instruments.

Overall, this move could only be construed as an attempt to increase the
profit margin of their service operation. The general purpose SEMs are
spread out geographically around the country, where the semi business is
far less so. The older instruments represent an additional training cost
for new service people. By limiting the number of instruments being
serviced, the geographical spread of those instruments and the learning
curve of their service staff, they can drastically cut back their service
staff, weather attrition and defections of service staff by having new
hires online quicker and in the end, probably have a staff that is paid
less to do more. The same could also be said of their sales force.

Don't sweat, Gary - cathode emitters are available for the Amray FESEMs in
the aftermarket for around $2,500 and turbopump exchanges cost about the
same from the manufacturer. I can't really address the pricing strategies
of other service providers, but in my case, whatever type of contract you
choose, or even on a billable basis, you would save at least a few thousand
a year over the Amray service costs. I'll cover the cathode by tacking on
the $2,500 per year to the contract and simply change it once a year, and
still beat Amray's cost by $4,000 - $6,000 per year. But I suggest that
the customer cover it themselves - buy a new emitter when they first take
out a contract and keep it on hand. In this way, the customer can
themselves better control the cost through proper care and operation of the
instrument, and deciding for themselves when a replacement is needed. This
also gives you that warm fuzzy feeling knowing that you have one standing
by, ready to use if needed. Third party service providers have great
flexibility in writing contracts - in the case above, I would normally
cover the cost of installation of the emitter even though you may have
chosen to be responsible for the emitter.

Your mention of those using the instrument having some ability to take care
of many problems they run across is right on the mark. I always prefer
customers who have a real interest and knowledge of the instrumentation
they use. Not because it may make my life easier (although it does because
I know I'm talking to someone knowledgeable and am more willing to take
what they say at face value), but because it's fun to share with, and learn
from, those with similar interests.

I'm all for having a customer watch over my shoulder and understand what
I'm doing, because it protects their better interests as well as mine. I'm
not perfect, and sometimes (perhaps often) I'll get off on the wrong
direction on a problem. The better the description of the problem I get,
the better I'll do. It also helps sometimes to have a back-seat driver to
let you know when you've taken a wrong turn, as damaging to the ego as that
can be. Of course, for the customer, having a better knowledge of the
instrument and its service means some peace of mind in finding someone to
service it.

Kind of like knowing something about the various systems and construction
methods in a house before hiring a remodeling company.

On Friday, March 23, 2001 8:32 PM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
}
} The word I have received so far is that later model FESEMs will be
} covered under contracts. The distinction of "later model" is
} a perhaps nebulous term. The 1800 series instruments
} will probably not qualify. My 1910FESEM is based on 1800
} electronics but uses the 305FE gun--and so far, is covered
} under contract. I am good for the rest of CY 2001. If Amray
} KLA stopped supporting FESEMs, something major would have
} to occur to keep the guns (at a minimum) available to established
} systems. The guns seem to last about 2.5-4 years. During
} that time, they are rock solid.
}
} It seems to me that the more that a microscopist can do
} in regards to maintenance is better in the near term. This
} is despite a maintenance contract. If simple things go
} wrong and you can fix them, doing this will keep your
} system below the cost accountant's radar screen. The key
} for having the contract is if something really major goes
} wrong. An FE gun under contract is about $2K. Otherwise,
} it is at least $18K. How about a turbo pump? No cost
} under contract. Otherwise???
}
} what about changing final apertures? I have not done a good
} job of doing this. But now I know more about how to do it
} correctly. And cleaning the holder is not a trivial process,
} nor one to be ignored.
}
} gary g.
}
}
} At 01:56 PM 3/23/2001, you wrote:
}
} }
} } Hi Listers,
} }
} } I have been receiving conflicting reports concerning Amray service on
the FE
} } SEMs.
} } The "official" word has been that Amray will continue to support the
FESEMs
} } "for some time" but I have had several customers call me & request
service
} } for their FESEMs as Amray will not offer a contract for the next year.
} }
} } Has anyone had a similar experience or give me a striaght answer on this
} } subject?
} }
} }
} } Thank You,
} }
} } Earl Weltmer
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sat Mar 24 21:35:03 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 24 Mar 2001 19:34:06 -0800
Subject: RE: Amray FE SEM Contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 03:31 PM 3/24/2001, you wrote:

} [snip]
}
} The only question that seems to remain is - what business is being shut
} down? Amray has always been a major player in the semi-conductor field.
} These days, the FESEM is the most attractive portion of that business. My
} guess would be that they have chosen to still pursue the semi manufacturers
} with FESEMs but close down their general purpose SEM business. In the mean
} time, they want to still provide the lucrative service contracts on the
} general purpose FESEMs out there. Cash is king, and the cash in their
} operation is in the selling and service of FESEMs, primarily to those
} customers who are willing and able to pay a premium for these instruments.

Amray, as a company, no longer exists. Amray is a division of KLA-Tencor.
For some reason, Amray apparently could not compete in the general
purpose (lab) SEM marketplace. Or, other forces came into play which
caused the change.

I do know that Amray had developed very good FESEM systems--optics,
control software, stages, etc. KLA-Tencor is in the semiconductor defect
analysis business. They lacked the type of SEM technology that Amray
had. Consequently, KLA-Tencor purchased Amray, ostensibly I believe,
to acquire Amray's SEM technology. This is similar to the relationship
between FEI and Philips, wherein Philips acquired FEI for its ion beam
expertise. To wit, FEI now produces a fine dual beam focused ion beam
system which combines ion beam technology and SEM imaging (e.g., FEI 830).

KLA-Tencor produces a top line semiconductor defect analysis tool set
(8100xl, 8300, 8500, etc.) which combines KLA's best attributes along
with the FESEM qualities of Amray. The defect analysis tools cost about
$2M each, compared to a typical lab FESEM at say $400K. The earlier
Amray systems were priced lower than this. It seems to me that KLA,
with Amray in tow, had made a decision to focus on the high cost (high
profit) business area of semiconductor defect analysis, at the exclusion
of lab SEMs. That is why we are seeing what is happening now with
Amray service contracts.

In some respects, this was a myopic decision. Currently, the semiconductor
industry is in a decline (albeit not monumental). There surely was and
is a market for lab SEMs. KLA obviously made a business decision
to focus on semiconductor defect analysis tools. From what I am seeing,
the older Amray systems are no longer being supported by Amray.
Since the thermionic systems are rather simple in design and easier
to maintain than FESEMs, this may not be too bad. But, if one had
a bad PC-10, that is of course a different matter.

So far as I can tell, Amray will support the FESEMs. Why they
would not support a 3300 eludes me. Perhaps that is the bow wave
of non-support for all Amray systems. I don't know. But I do know
that a new Philips, Jeol, or Hitachi system at say $400K+ is about
triple what I have invested in my current Amray FESEM. As long
as my Amray system does what I need and can be maintained to do it,
it makes no economic sense for me to dump it for a horribly
expensive replacement. I suppose that what may happen is that
the few lab SEM makers will offer about the same compliment of systems at
about the same price mix. And the prices will be painful.

I have seen this same type of consolidation in the UK and other
countries. For us Amray owners, it is a real problem....conundrum?

gary g.



From daemon Sat Mar 24 21:38:57 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 24 Mar 2001 23:33:30 -0600
Subject: RE: Amray FE SEM Contracts

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Allen,

Who has the "aftermarket" FE tips?

Regards,

Earl

----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Gary Gaugler'" {gary-at-gaugler.com} ; "Earl Weltmer" {earlw-at-pacbell.net}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, March 24, 2001 3:31 PM


Didn't mean to leave you with any misconceptions. I'm aware of the
purchase by KLA and as far as I am aware, any decisions made were theirs.
To date, as far as I know, sales have still been under Amray brand name,
which is why I referred to them that way. I believe that there were some
non-general purpose SEM technologies that KLA was primarily interested in
and will roll those into its own production. Perhaps Amray was poised to
take a firmer stance in those areas? As far as Amray, it has always been
more of a family run business and perhaps the offer was right and the time
was right. I don't think there were any particular problems in the
operation or bottom line of Amray.

I also didn't mean to leave anyone with the impression that they are not
supporting the 3300. On the contrary, what I wrote was that they were
upset that anyone would consider a third-party service provider for one.
They were quite upset that they had lost one.


On Saturday, March 24, 2001 9:34 PM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} At 03:31 PM 3/24/2001, you wrote:
}
} } [snip]
} }
} } The only question that seems to remain is - what business is being shut
} } down? Amray has always been a major player in the semi-conductor field.
} } These days, the FESEM is the most attractive portion of that business.
My
} } guess would be that they have chosen to still pursue the semi
manufacturers
} } with FESEMs but close down their general purpose SEM business. In the
mean
} } time, they want to still provide the lucrative service contracts on the
} } general purpose FESEMs out there. Cash is king, and the cash in their
} } operation is in the selling and service of FESEMs, primarily to those
} } customers who are willing and able to pay a premium for these
instruments.
}
} Amray, as a company, no longer exists. Amray is a division of
KLA-Tencor.
} For some reason, Amray apparently could not compete in the general
} purpose (lab) SEM marketplace. Or, other forces came into play which
} caused the change.
}
} I do know that Amray had developed very good FESEM systems--optics,
} control software, stages, etc. KLA-Tencor is in the semiconductor defect
} analysis business. They lacked the type of SEM technology that Amray
} had. Consequently, KLA-Tencor purchased Amray, ostensibly I believe,
} to acquire Amray's SEM technology. This is similar to the relationship
} between FEI and Philips, wherein Philips acquired FEI for its ion beam
} expertise. To wit, FEI now produces a fine dual beam focused ion beam
} system which combines ion beam technology and SEM imaging (e.g., FEI
830).
}
} KLA-Tencor produces a top line semiconductor defect analysis tool set
} (8100xl, 8300, 8500, etc.) which combines KLA's best attributes along
} with the FESEM qualities of Amray. The defect analysis tools cost about
} $2M each, compared to a typical lab FESEM at say $400K. The earlier
} Amray systems were priced lower than this. It seems to me that KLA,
} with Amray in tow, had made a decision to focus on the high cost (high
} profit) business area of semiconductor defect analysis, at the exclusion
} of lab SEMs. That is why we are seeing what is happening now with
} Amray service contracts.
}
} In some respects, this was a myopic decision. Currently, the
semiconductor
} industry is in a decline (albeit not monumental). There surely was and
} is a market for lab SEMs. KLA obviously made a business decision
} to focus on semiconductor defect analysis tools. From what I am seeing,
} the older Amray systems are no longer being supported by Amray.
} Since the thermionic systems are rather simple in design and easier
} to maintain than FESEMs, this may not be too bad. But, if one had
} a bad PC-10, that is of course a different matter.
}
} So far as I can tell, Amray will support the FESEMs. Why they
} would not support a 3300 eludes me. Perhaps that is the bow wave
} of non-support for all Amray systems. I don't know. But I do know
} that a new Philips, Jeol, or Hitachi system at say $400K+ is about
} triple what I have invested in my current Amray FESEM. As long
} as my Amray system does what I need and can be maintained to do it,
} it makes no economic sense for me to dump it for a horribly
} expensive replacement. I suppose that what may happen is that
} the few lab SEM makers will offer about the same compliment of systems at
} about the same price mix. And the prices will be painful.
}
} I have seen this same type of consolidation in the UK and other
} countries. For us Amray owners, it is a real problem....conundrum?
}
} gary g.
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sun Mar 25 09:47:15 2001



From: paleomac-at-kscable.com
Date: Sun, 25 Mar 2001 09:43:11 -0600
Subject: Ask-A-Microscopist:LM Course

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Email: paleomac-at-kscable.com
Name: Larry McCoy

Organization: Retired

Education: Undergraduate College

Location: Topeka,Ks.,USA

Question: I recently purchased a microscope used in forensics with a state
agency. I would like to study microscopy so I could show this fascinating
subject to my grandchildren. As a youngin' I enjoyed biological and
petrology studies with the microscopes. I'm looking for a course that
would allow me to use the different features of the microscope in light and
dark field applications. I'm retired and would appreciate any help in this
endeavor that you could offer. I am suffering from a physical disability
and erudition is how I go about studies these days. THANX again.

---------------------------------------------------------------------------




From daemon Sun Mar 25 12:29:04 2001



From: James Talbot :      james-at-ktgeo.com
Date: Sun, 25 Mar 2001 12:15:47 -0600
Subject: RE xRD Equipment

Contents Retrieved from Microscopy Listserver Archives
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David-

For a fairly comprehensive list try
http://www.xraysite.com/index.html?producers.html

I have experience with diffractometers made by Rigaku, Scintag and
Siemens - all
have good systems.

I have also dealt with Jim Stauver at JSTechnical. He sells and
services
Siemens systems and can probably get you a good deal on a used system.
His web
site is http://www.jstechnicalservices.com/

If you need any more info or help, contact me off-list.

Sincerely,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(214) 403-6342
visit my web site at http://www.ktgeo.com/



"Ziegler, David SBCCOM(N)" wrote:

}
------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.

}
} Hello listers
}
} I've been asked to look into XRD equipment for our lab here, and was
} wondering if any of you know who the major players are in this area.
We
} deal mostly with polymers and polymer fibers, and want to be able to
do both
} small and wide angle work.
}
} Thanks for the help.
} dz
}
} David Ziegler
} U.S. Army, SBCCOM
} AMSSB-RSS-MS(N)
} Materials Science Team, SS&T
} Natick, MA 01760-5020
} TEL: (508) 233-6484
} FAX: (508) 233-5521
} Email: David.Ziegler-at-Natick.Army.Mil




From daemon Sun Mar 25 19:34:19 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 25 Mar 2001 17:24:40 -0800
Subject: Re: Ask-A-Microscopist:LM Course

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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Larry -

You're not lilely to find a "course" that will meet your needs. Try
starting with this book; this listing is taken from the Project MICRO
bibliography, which you will find at
http://www.msa.microscopy.com/ProjectMICRO/PMBooks.html

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

If you want a more sophisticated, interactive (and more expensive)
approach, try this CD-ROM (from the same bibliography):

Pagliaro, l., Murray, C., Curran, G., Orkand, A., and Astion, M. 1997
Microscopy-Tutor. ISBN 0-7817-1217-3 $195.00 plus shipping; distributed
by Lippincott-Raven Publishers, 12105 Insurance Way, Hagerstown, MD 21740,
800-638-3030. For Macintosh and Windows 3.1 or 95/NT; easy installation.
Developed by the Department of Laboratory Medecine and the Center
for Bioengineering at the University of Washington, Seattle, this is a
college-level introduction to the use of a research-quality compound
microscope. Its extensive use of QuickTime animation to illustrate
alignment steps and optical principles make it much more than a "book on a
CD"; moving ripples on a pond really do make it easier to understand wave
theory! Kohler illumination is emphasized and explained. Most, but not
all, terminology is defined; a glossary would help beginners. Proper care
of lenses is covered well, but there's no instruction on how to use
immersion oil properly. There is a brief concluding self-test that is more
of a review of important information than a quiz. Although it's a good CD,
it's definitely not appropriate for precollege microscopists. Adult.

And there are dozens of things listed for the grandkids...


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Mar 26 05:01:17 2001



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Mon, 26 Mar 2001 11:53:56 +0100
Subject: GACH Resin

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Dear Listers,

Having advised one of our customers to try the above resin
(glutaraldehyde/carbohydrazide mixture) to embed her particularly sensitive
material for LM (it was dissolving using other mainstream embedding media),
she has now successfully got to the sectioning stage. Next problem is
getting the sections to stick to the microscope slide. They curl and crack
and disappear Lemming like into the abyss.

Having cut resin sections for many years I have now exhausted all the
tricks that I have used to keep them on the slide from poly-L-lysine to
silane to drying in an oven overnight (instead of using a hotplate or slide
dryer). That last advice has surprisingly saved many desperate
microscopists but not this time. Maybe one of the adhesive sticks which
lays down a sticky membrane will work. We will even try double sided tape
(or maybe not), but does anyone out there have experience with sections cut
from this material? Help,

Regards

Terry Cooper
TAAB Laboratories Equipment Ltd
England


From daemon Mon Mar 26 08:17:49 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Mon, 26 Mar 2001 09:11:34 -0500
Subject: Thanks to all.

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who offered help on the subject of the high resolution image
if the MSA logo. Most thanks go to Bev Maleef who provide the goods.

I did have someone offer to take a micrograph of it with an SEM! I didn¹t
know we actually had a copy ofthe MSA logo that small, has any one been
playing the STM tricks with the individual atoms a la IBM?

--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"




From daemon Mon Mar 26 08:28:06 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 26 Mar 2001 08:23:59 -0600
Subject: Re: GACH Resin

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
I have found that polyethlyene-imine (PEI) is much stickier
than poly-lysine. I have no idea about the resin you mentioned, but
PEI is sticky stuff. It comes as a liquid. I make a 0.1% soloution in
ddwater, which I freeze in aliquots. Then I keep a working one in the
fridge. I coat coverslips in the stuff by floating them on a drop of
the PEI solution for about 10 sec, and then blotting off the excess
and letting them air dry. In that conditions, the coated 'slips are
good for at least months.

Hope this helps,
Tobias Baskin


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Mon Mar 26 09:31:51 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 26 Mar 2001 10:34:55 -0500
Subject: Cryomicrotome

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
Can anyone in the Atlanta area help with this request? Please send your
replies to Ulrika - E-mail: Ulrika.Egertsdotter-at-ipst.edu.
thanks for your help,
Beth
Here's the request:
} I am an
} assistant scientist currently working at the Institute of Paper science and
} technology in Atlanta and I am trying to find a cryomicrotome that I could
} use for my project. Within my project, we are broadly speaking trying to
} reveal information about the biological mechanisms of wood formation. We
} are using different types of arrays, and for the sample preparations we are
} trying to get as thin sections as possible. For this purpose I am now
} looking for a cryomicrotome that I could access to make thin sections of
} wood samples that would then be processed for RNA to probe the arrays, and
} also hopefully in situ hybridisation. I am planning to go to Sweden to
} learn the techniques from the group that has already been doing this type
} of work in poplars. I will however need to find an accesible cryomicrotome
} to work with that are somewhat closer to Atlanta. Do you think it would be
} possible at all for me to make use of the cryomicrotome in your department,
} or would you perhaps know of any other available equipment?
}
} Thank you.
}
} Sincerely,
} Ulrika Egertsdotter
} E-M Ulrika Egertsdotter, Ph.D.
} Institute of Paper Science and Technology
} 500 10th Street, N.W.
} Atlanta, GA 30318
}
} Direct dial: +1 404 894 7842/4807
} Telephone: +1 404 894 5700
} Fax. +1 404 894 4778
} E-mail: Ulrika.Egertsdotter-at-ipst.edu


**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Mon Mar 26 09:49:49 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Mon, 26 Mar 2001 10:47:19 -0500
Subject: Bake out

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,

I am very new to electron microscopy, so I ask your indulgence in some basic
questions.

I have an old JEOL SEM that has been sitting at atmospheric pressure for
some time now and will need a thorough baking. It has a plain old tungsten
wire filament, so the vacuum requirements aren't all that stringent. The SEM
has rubber o-rings (I don't know how to tell if they are Viton). I doubt
that I want to go above 100 oC.

1) What characteristics are important in choosing a heat tape suitable for
baking the instrument?

2) Where would I find a suitable heat tape (my web searches on heat tapes
got me lots of information about ice buildup on my roof, but very little
about getting water out of an SEM)?

3) How do I determine how much to buy - how much column on each side of the
tape can I assume to be heated properly by the tape - what is an appropriate
center-to-center distance between adjacent wraps of tape?

4) Is there a more practical method than heat tapes for baking the
instrument?

5) What else may I be missing here?

Thank you,

Bruce Girrell
in snowy northern Michigan with a few more basic questions waiting in the
wings



From daemon Mon Mar 26 09:49:50 2001



From: R. Howard Berg :      rhberg-at-danforthcenter.org
Date: Mon, 26 Mar 2001 09:46:58 -0600
Subject: Re: LM-staining lignin

Contents Retrieved from Microscopy Listserver Archives
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The Sudan dyes are those most commonly used for suberin, and perhaps cutin.
The lignin component of both can be stained with phloroglucinol (or
observed as autofluroescence with blue excitation in unstained material).
If the autofluroescence is quenched by the Sudan, this supports the
interpretation that the substance is suberin or cutin rather than lignin.



} ------------------------------------------------------------------------
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R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility,
Associate Member
Donald Danforth Plant Science Center/Nidus Center
893 North Warson
St. Louis, MO 63141

phone: 314-812-8076
fax: 314-812-8127
cell phone: 314-378-2409

http://www.danforthcenter.org





From daemon Mon Mar 26 09:50:23 2001



From: Louis Somlai :      lssomlai-at-www.psrc.usm.edu
Date: Mon, 26 Mar 2001 09:49:50 -0600 (EST)
Subject: TEM stain; nylon sample

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Would anyone know of a general protocol I could use to stain a nylon 6,6
sample for TEM analysis? I'm studying cross-sections of nylon fibers. I've
read that tin chloride is a good stain for nylons, I've also seen
reference to work using iodine.

Thanks,

Louis



From daemon Mon Mar 26 10:08:25 2001



From: NPGSlithography-at-aol.com
Date: Mon, 26 Mar 2001 11:04:28 EST
Subject: Re: SEM digitalizer

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 3/23/2001 1:35:13 PM Mountain Standard Time,
pogany-at-power.szfki.kfki.hu writes:

} I'm seeking a controller for a JSM840 whicsh is reliable and can control
} the SEM beam in X-Y direction at least with 4096 pixel resolution.

A list of providers of digital imaging systems is available at:

http://www.jcnabity.com/links.htm#Digital Imaging

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Mon Mar 26 10:33:46 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 26 Mar 2001 11:13:50 -0500
Subject: RE: Bake out

Contents Retrieved from Microscopy Listserver Archives
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I would not recommend baking out that system if it has not been designed for it. The heat tapes will give you hot spots and other places may not take the heat well. If you are not going above 100C, it is not going to help you much anyhow. At least 200C is required for modest bakes. The purpose of a bakeout is to get water off the walls and you have to overcome the heat of chemsorption.

A better way is to rig a leak of nitrogen from a liquid nitrogen container (open to atmosphere) so that you get flow in the viscous flow range. You have to throttle your vacuum pump so if you do not have a way to do this with your high vacuum pump, do it with your mechanical pump and throttle your leak. I think that if your run at about 0.1 Torr into a typical 1.5 inch vacuum line that you will be in the viscous range.

If you use clean stainless steel or copper tubing that is coiled and put your heat tape around that, you can heat the nitrogen gas going into your system for added benefit. Run this leak overnight and it will take out all the water off of the walls.

There is a commercially available system to do this and more. Contact Ron Vane at XEI.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Bruce Girrell [mailto:bigirrell-at-microlinetc.com]
} Sent: Monday, March 26, 2001 10:47 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Bake out
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Greetings,
}
} I am very new to electron microscopy, so I ask your
} indulgence in some basic
} questions.
}
} I have an old JEOL SEM that has been sitting at atmospheric
} pressure for
} some time now and will need a thorough baking. It has a plain
} old tungsten
} wire filament, so the vacuum requirements aren't all that
} stringent. The SEM
} has rubber o-rings (I don't know how to tell if they are
} Viton). I doubt
} that I want to go above 100 oC.
}
} 1) What characteristics are important in choosing a heat tape
} suitable for
} baking the instrument?
}
} 2) Where would I find a suitable heat tape (my web searches
} on heat tapes
} got me lots of information about ice buildup on my roof, but
} very little
} about getting water out of an SEM)?
}
} 3) How do I determine how much to buy - how much column on
} each side of the
} tape can I assume to be heated properly by the tape - what is
} an appropriate
} center-to-center distance between adjacent wraps of tape?
}
} 4) Is there a more practical method than heat tapes for baking the
} instrument?
}
} 5) What else may I be missing here?
}
} Thank you,
}
} Bruce Girrell
} in snowy northern Michigan with a few more basic questions
} waiting in the
} wings
}
}


From daemon Mon Mar 26 11:51:51 2001



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Mon, 26 Mar 2001 13:46:21 -0400
Subject: TEM: CCTV system available

Contents Retrieved from Microscopy Listserver Archives
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Greetings:

We have recently decommissioned a Fullam closed circuit TV imaging system,
purchased in the late 80's-early 90's for use on a JEOL-1200EX. The system
is in excellent condition, includes a side-port camera mount and detector,
Dage MTI 70 camera, Nikkor lens, etc., and is available for donation to a
school or university.

If interested, please respond directly to me and not to the list.

Regards,
Bev Maleeff

GlaxoSmithKline Pharmaceuticals
Safety Assessment
709 Swedeland Road
M/C UE 0462
King of Prussia, PA 19406

610-270-7987
610-270-7202 fax
Beverly_E_Maleeff-at-sbphrd.com



From daemon Mon Mar 26 12:17:59 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Monday, March 26, 2001 9:22 AM
Subject: RE: Bake out

Contents Retrieved from Microscopy Listserver Archives
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Microscopy list and Bruce:

I think a bigger question here is why Bruce thinks he needs a bake out? If
he "heard" from someone that is what you have to do for a vacuum system then
we should give him some easier advice.

First, we must all understand that most SEMs are not ultra high vacuum
system that require baking. SEMs have specimen chamber with a typical
vacuum between 10-5 to 10-6 Torr. High vacuum at best. An old JEOL SEM was
never baked. Water vapor does not bother the electron beam or the secondary
electrons at these pressures. I would clean the vacuum system with IPA,
check and change as needed the pump oil, and try pumping down. Then I would
fix any leaks.

Bruce, have you tried to pump it down? What make you think you should bake
it? If oily dirt is a problem, then nitrogen purging like Scott Walck
suggests might be a solution after you wipe down the chamber wall with IPA.

I have a long discussion of oil and hydrocarbon contamination and removal
methods at www.SEMCLEAN.Com.

Ronald Vane
XEI Scientific
Notice: XEI makes anti-contamination system for SEMs.




Bruce have you tried pumping it down?


-----Original Message-----
} From: Walck, Scott D. {walck-at-ppg.com}
To: 'bigirrell-at-microlinetc.com' {bigirrell-at-microlinetc.com}
Cc: 'Microscopy' {microscopy-at-sparc5.microscopy.com}



From daemon Mon Mar 26 12:48:05 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Mon, 26 Mar 2001 13:45:32 -0500
Subject: Bake out

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I appreciate the many responses that I have gotten already.

Several have questioned the need for a bake out. Well, I did say I was new
to this...

The reasons I felt that the instrument should be baked are:
1) Wilbur Bigelow, in _Vacuum Methods in Electron Microscopy_ suggests that,
short of pumping endlessly, baking is almost a necessity to remove adsorbed
water.
2) The instrument has been sitting at atmospheric pressure for an untold
period of time, at least several months. The E-T detector had been removed,
leaving a nice 6" dia. hole in the system. God knows what else besides water
vapor is in there.
3) The logbook that came with the unit recorded routine baking. Along with
item 1), I concluded that baking was simply just a matter of course.

What I am hearing so far is that 1) for the relatively low vacuum required
for this instrument, baking may not be necessary and 2) short of going to
about 200C, baking may not be very effective.

Bruce Girrell




From daemon Mon Mar 26 13:40:21 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Mon, 26 Mar 2001 13:35:02 -0600
Subject: RE: Bake out

Contents Retrieved from Microscopy Listserver Archives
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Baking won't be necessary, you're biggest problem will be the o-ring seals.
I assume this is a diffusion pump system.

O-ring seals have a tendency to dry out and shrink, if they've been in the
system for some time, they will also have been shaped by the pressure of
the flanges. The result will be poor mating with the flanges and lots of
leaks.

You'll need to take each one out of the system and inspect it for drying
and cracking. I do mean each one - if the system has been at atmosphere
for a long time, you'll want to get every o-ring that seals the sample
stage controls, vacuum valves and the gun translation mechanism, if so
equipped.

If the o-rings are not too badly out of round and not cracking, then they
can generally be reconditioned by cleaning them off with acetone and
applying a very light layer of vacuum grease evenly on the surface. The
grease is only to seal the surface, prevent drying and increase
flexibility. I use acetone, although many will probably warn you not to,
because it slightly swells the o-ring. When you're doing a general
reconditioning like this, that helps the o-rings seal up right away, but it
will still take a day or two at high vacuum to get them to fully seal.

This is also a good time to get in and clean the column and wipe down the
sample and gun chambers. It's hard to say what will work here for you, as
it depends largely on the pump oils that have been used in the past and the
accumulated contamination from samples. Try some standard solvents like
methanol, ethanol, acetone, etc. Hopefully one or a combination can remove
some of the build-up.

The outgassing rates of adsorbed gasses at the vacuum levels you'll attain
in the instrument are not really a problem. You'll want to leave the
instrument in high vacuum for at least 24 hours. Over the first day or
two, you will notice that the vacuum level slowly improves, primarily due
to the o-rings being sucked into better contact with the flanges. Over
this time, the system will also reach equilibrium with outgassing products.


On Monday, March 26, 2001 9:47 AM, Bruce Girrell
[SMTP:bigirrell-at-microlinetc.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings,
}
} I am very new to electron microscopy, so I ask your indulgence in some
basic
} questions.
}
} I have an old JEOL SEM that has been sitting at atmospheric pressure for
} some time now and will need a thorough baking. It has a plain old
tungsten
} wire filament, so the vacuum requirements aren't all that stringent. The
SEM
} has rubber o-rings (I don't know how to tell if they are Viton). I doubt
} that I want to go above 100 oC.
}
} 1) What characteristics are important in choosing a heat tape suitable
for
} baking the instrument?
}
} 2) Where would I find a suitable heat tape (my web searches on heat tapes
} got me lots of information about ice buildup on my roof, but very little
} about getting water out of an SEM)?
}
} 3) How do I determine how much to buy - how much column on each side of
the
} tape can I assume to be heated properly by the tape - what is an
appropriate
} center-to-center distance between adjacent wraps of tape?
}
} 4) Is there a more practical method than heat tapes for baking the
} instrument?
}
} 5) What else may I be missing here?
}
} Thank you,
}
} Bruce Girrell
} in snowy northern Michigan with a few more basic questions waiting in the
} wings
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Mon Mar 26 14:45:43 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 26 Mar 2001 14:41:06 -0600
Subject: AFM: estimate general operating expenses

Contents Retrieved from Microscopy Listserver Archives
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We are relatively new to atomic force microscopy and need some
estimate of costs for routine maintenance. I don't mean expendible
items but repairs.

For example, we have been facing some (in my opinion) rather high
costs to repair hardware: broken scanner $3,900, electronic module
$400 (twice). Are AFM's prone to such frequent, high repair costs?

Has anyone ever had to replace a scanner that "broke" for no apparent
reason? BTW, none of these repairs were caused by operator error.

Just wondering.......

Thank you,

John

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Mon Mar 26 15:03:00 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 26 Mar 2001 16:03:11 -0500
Subject: Re: Bake out

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In conducting RGA studies of polymer outgassing inside hermetic
electronic packages some years ago we found that hydrogen seems to have
the effect of displacing moisture adsorbed onto metal surfaces. It was
a problem that amounted to a "memory effect" within the RGA* for us but
the effect can be used to advantage in displacing water. You might try
Scott's suggestion substituting bottled, high purity hydrogen for the
liquid nitrogen bleedoff. Obviously, hydrogen is flammable and requires
caution when in use.

(* e.g. if a hermetic package punctured in the RGA sample compartment
contained moisture, some of the released water vapor would adsorb onto
the chamber wall. If the next hermetic package contained hydrogen, it
would displace the moisture from the previous package causing it to be
attributed to the second unit when, in fact, it was left over from the
first. During pumping out of the sample compartment in between samples,
the background level of moisture would be achieved even though it was
lurking on the metal surface waiting to be liberated.)

John Twilley
Conservation Scientist

Walck, Scott D. wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I would not recommend baking out that system if it has not been designed for it. The heat tapes will give you hot spots and other places may not take the heat well. If you are not going above 100C, it is not going to help you much anyhow. At least 200C is required for modest bakes. The purpose of a bakeout is to get water off the walls and you have to overcome the heat of chemsorption.
}
} A better way is to rig a leak of nitrogen from a liquid nitrogen container (open to atmosphere) so that you get flow in the viscous flow range. You have to throttle your vacuum pump so if you do not have a way to do this with your high vacuum pump, do it with your mechanical pump and throttle your leak. I think that if your run at about 0.1 Torr into a typical 1.5 inch vacuum line that you will be in the viscous range.
}
} If you use clean stainless steel or copper tubing that is coiled and put your heat tape around that, you can heat the nitrogen gas going into your system for added benefit. Run this leak overnight and it will take out all the water off of the walls.
}
} There is a commercially available system to do this and more. Contact Ron Vane at XEI.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
}
}
} } -----Original Message-----
} } From: Bruce Girrell [mailto:bigirrell-at-microlinetc.com]
} } Sent: Monday, March 26, 2001 10:47 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Bake out
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
} }
} }
} }
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } Greetings,
} }
} } I am very new to electron microscopy, so I ask your
} } indulgence in some basic
} } questions.
} }
} } I have an old JEOL SEM that has been sitting at atmospheric
} } pressure for
} } some time now and will need a thorough baking. It has a plain
} } old tungsten
} } wire filament, so the vacuum requirements aren't all that
} } stringent. The SEM
} } has rubber o-rings (I don't know how to tell if they are
} } Viton). I doubt
} } that I want to go above 100 oC.
} }
} } 1) What characteristics are important in choosing a heat tape
} } suitable for
} } baking the instrument?
} }
} } 2) Where would I find a suitable heat tape (my web searches
} } on heat tapes
} } got me lots of information about ice buildup on my roof, but
} } very little
} } about getting water out of an SEM)?
} }
} } 3) How do I determine how much to buy - how much column on
} } each side of the
} } tape can I assume to be heated properly by the tape - what is
} } an appropriate
} } center-to-center distance between adjacent wraps of tape?
} }
} } 4) Is there a more practical method than heat tapes for baking the
} } instrument?
} }
} } 5) What else may I be missing here?
} }
} } Thank you,
} }
} } Bruce Girrell
} } in snowy northern Michigan with a few more basic questions
} } waiting in the
} } wings
} }
} }
}
}
}



From daemon Mon Mar 26 15:32:30 2001



From: Haifeng.Wang-at-ReadRite.com
Date: Mon, 26 Mar 2001 13:30:04 -0800
Subject: X-ray radiation detector for TEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a X-ray radiation detector used on TEMs. Currently we have
a Geiger counter that is only sensitive to low energy X-ray. The purpose of
the new detector is to sense high energy X-ray leakage from a 200 kV scope.

Any suggestion or clue will be greatly appreciated. Please contact off-line.

Haifeng.Wang-at-readrite.com



From daemon Mon Mar 26 16:25:45 2001



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 26 Mar 2001 17:18:36 -0500 (EST)
Subject: Re: ? how to make lacey carbon films?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There's a whole chapter (#4) on support films in Hayat MA, Miller SE.
1990. Negative Staining. Pages 201-214 are on perforated films and
discuss making of films with different size holes.


On Fri, 23 Mar 2001, Gordon Vrololjak wrote:

} X-Sieve: cmu-sieve 1.3
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} Fri, 23 Mar 2001 20:14:59 -0500 (EST)
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} Fri, 23 Mar 2001 20:11:33 -0500 (EST)
} Received: (from daemon-at-localhost)
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} for dist-Microscopy; Fri, 23 Mar 2001 17:51:15 -0600 (CST)
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} by ultra5.microscopy.com (8.9.3+Sun/8.9.1) with ESMTP id RAA13763
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} by nature.Berkeley.EDU (Postfix) with ESMTP id D3AB54DB35
} for {microscopy-at-sparc5.microscopy.com} ; Fri, 23 Mar 2001 15:48:36 -0800 (PST)
} Date: Fri, 23 Mar 2001 15:48:36 -0800 (PST)
} From: Gordon Vrololjak {gvrdolja-at-nature.Berkeley.EDU}
} To: {microscopy-at-sparc5.microscopy.com}
} Subject: ? how to make lacey carbon films?
} Message-ID: {Pine.GSO.4.33.0103231546140.28537-100000-at-nature.Berkeley.EDU}
} MIME-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
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}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Hello Everyone,
} I want to make my own lacey carbon films for cryo-tem and was wondering if
} anyone had some good methods, experiences, insights, or references?
}
} I found references for lacey formvar, pure carbon films, and a few
} others... But nothing explicitely for lacey carbon.
}
} All comments greatly appreciated, Thanks.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon Mar 26 16:26:52 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 26 Mar 2001 16:21:28 -0600
Subject: PSF slides

Contents Retrieved from Microscopy Listserver Archives
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I would appreciate hearing how the experts out there prepare their
microscope slides for measuring a PSF. We got a couple of prepared
slides from Molecular Probes and were quite disappointed with the
quality of the Blue and Green beads (the red ones were fine). This
was somewhat surprising since they usually have great quality. We
bought the beads and are making our own but I would be interested in
any protocols or alternative sources for prepared slides. Thanks in
advance, Tom




--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Mon Mar 26 17:27:39 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 26 Mar 2001 15:27:04 -0800
Subject: Re: Bake out

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A W gun system doesn't need a particularly high vacuum to
operate. But a better vacuum will extend the life of the filament
dramatically. While I am not familiar with your JEOL instrument,
I have worked with Amray 1610T and 1830 systems which had
an ion pump so a LaB6 cathode could be used. Each system
had separate guns for W and LaB6. I only ran W in the 1610T
but only LaB6 in the 1830. In this respect, the ion pump had to
be working and the vacuum had to be quite good.

When the gun chamber was opened, either for column liner
cleaning, liner aperture change, anode aperture change or
for cathode change and Wenhelt change, I baked out the
gun chamber. If the ion pump indicated an inability to pump
down quickly, I baked it out too. I never baked the chamber.
Usually, when a system would sit around without being under
vacuum, a long pumping after internal wipe down with methanol would
bring the chamber vacuum back. If not, that is unchartered
territory for me.

I baked out the gun chamber and the ion pump at 175F. All
pumps (except ion) were on during the bake. I used flexible
heating tape made by BH Thermal http://www.bhthermal.com
and their control thermostat. The thermostat control is
PN TS0991-550 and has a fluid bulb sensor. I used two
silicone rubber extruded heating tapes, PN BS0101060L
(313 Watts, 1" x 72") for gun and PN BS0201040L (418 Watts,
2" x 48") for pump. The first tape cost $95 and the second was $135
list. The controller was about $175.

The tape is wound tightly around the chamber or pump
and taped down with high temperature fiberglass tape.
This is PN PSAT36 (1/2" x 180ft) and costs $15 a roll.
PN AAT260 (2" x 180ft) is also handy...$61 per roll.
Wrapped around the heating tape is thick aluminum foil
(cooking variety) and taped tight to the heating tape.
The sensor bulb is taped down to the top of the gun
assembly and should be enclosed by the aluminum foil.
This makes it a set and forget deal for overnight baking.

The gaskets in my systems were Viton and copper.
At 175F, I suspect rubber components would be OK.
As far as I know, new replacements are Viton these days.
But I can't say for certain if your system has rubber or
Viton or even if replacements are Viton. Try heating
a spare gasket or O-ring enclosed in aluminum foil
in an oven at 175F and see if it is destroyed. That
ought to tell the story. If the logs say that the system
was baked, 175F ought to be safe.

The difference baking made was quite remarkable. If I
did not bake, the ion pump would draw about 100-120uA
and get worse as the cathode was on for much time.
After baking, it idled at 50-60uA and would stay there
for a long time.

The problem with a W-only system is that the gun chamber
is typically connected to the specimen chamber via a stand pipe or
some other vacuum connection. This means that the gun
area is basically at the vacuum level of the chamber. Thus,
my discussion of baking the gun chamber may be useless
to you. But you could apply the same baking principles to
the chamber and the gun chamber. I would not recommend
at all baking or heating the column. This would surely destroy
the scan coils.

If you have a turbo pump system, it is probably worth baking
the system to purge the trapped moisture. If not, then it
may not be of any value to bake. What kind of pumps does
the system have?

Hope this is a good starting point for you.

gary g.



At 07:47 AM 3/26/2001, you wrote:
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From daemon Mon Mar 26 18:19:10 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 26 Mar 2001 19:15:03 -0500
Subject: Need for coater parts: E5200

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Does anyone know where one could get a microprocessor of the type that was
used in the Polaron E5200 automatic coater. This was their first version
of an automatic coater and the replacement part is no longer available via
the manufacturer. Please respond to me off-line since this topic is
probably not of interest to many followers of this list. If anyone is
interested in the responses, let me know and I will send a summary of
anything I learn.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
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==================================================



From daemon Tue Mar 27 07:34:03 2001



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Tue, 27 Mar 2001 14:21:41 +0100
Subject: GACH resin

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

Thanks to everyone who sent me their ideas for adhering sections to slides.
We have had lots of suggestions and I will certainly post any successful
results we have from the input,

Thanks and best regards

Terry Cooper
TAAB Laboratories Equipment Ltd


From daemon Tue Mar 27 08:18:09 2001



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Tue, 27 Mar 2001 08:14:44 -0600
Subject: Cancellations for 3D Live-Cell Course mean space for you

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

As sometimes happens, some people find they can't get money to attend
courses at the last minute while others don't hear about the courses
until it is too late to apply.

Now may be your chance!

Several late cancellations at the UBC Live-Cell course mean three
opportunities for those of you who have ten days free this June. In
addition, the presence of more microscopy systems allows us to set up
one more group-of-three.

We expect to have an international faculty of 15, and on-site
equipment that includes 2-photon systems from Zeiss and Bio-Rad.
There will also be single-photon confocals from Bio-Rad, Olympus,
Nikon, Yokogawa/Solamere and Yokogawa/EG&G as well as deconvolution
set-ups from Applied Precision Instruments,
AutoQuant/Universal-Imaging, Improvision, Intelligent-Imaging-
Innovations and Zeiss, all set up for "live-cell" work. In addition,
there will be a laser tweezers/scissors from Cell-Robotics,
microinjection from Eppendorf and sundry other delights including a
fully equipped cell biology lab (with tireless technician!!) just for
students.

These microscopy systems are not just to look at but to use for over
45 hours of organized 2D and 3D live-cell laboratory sessions, plus
10 hours of evening sessions for group live-cell projects.

Although the eight "Groups-of-3" have already been set up and have
chosen their "individual projects," we are able to accept 3-2 more
students as long as their backgrounds will fit into one of these
groups, and we may even be able to set up one additional group.

If this interests you, go and find out more at:

http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

which has the rest of the story, including a preliminary program.

Then fill out the Registration Form from the site to tell us about
you, Fax it to Dr. Elaine Humphrey ASAP and we will see if we can fit
you in. (I will be away April 1-14)

Hope that you can join us in Vancouver this June 18-28.

Jim Pawley, Organizer
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Tue Mar 27 09:43:45 2001



From: A. Greene :      ablue-at-io.com
Date: Monday, March 26, 2001 8:27 PM
Subject: Bake out

Contents Retrieved from Microscopy Listserver Archives
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Hello Bruce,

As pointed out by many listers, it may not be necessary to bake your
instrument. Since the baking part has been well covered, I'll just add a
very effective way to tell Viton o-rings from rubber ones. If you can find
some Freon TF just put some in a beaker and drop in the questionable
o-ring. If the o-ring floats, it is rubber and if it sinks, it is Viton.

Good luck,
Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone 512/282-5507 FAX 512/280-0702

Sustaining Member - MICROSCOPY SOCIETY OF AMERICA

-----Original Message-----
} From: Bruce Girrell {bigirrell-at-microlinetc.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Tue Mar 27 14:38:33 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 27 Mar 2001 14:33:15 -0600
Subject: measureing micrscope stage movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
I am taking overlapping video images of a growing root
through a horizontal light microscope. For analysis, I need to put
the images back in register. I have tried incorportating various high
contrasty things into the background but this introduces various
problems ranging from death to the root to requiring a large overlap
between successive images. As an alternative, I thought of measuring
the displacement of the stage. A typical displacement between images
is around 300 microns and I need to measure this displacement to
within around 1 micron. I only need to work in one dimension, which
can be parallel to one of the edges of the stage. I have in mind
something like a "giant" afm detection set up, with a laser bouncing
off the edege of stage and picked up by a detector. But this is just
wild imagination on my part. Anyone out there have any *knowledge*
about how this might be accomplished?

Thanks in advance,
Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Tue Mar 27 18:06:52 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 27 Mar 2001 18:04:56 -0600
Subject: I was a student intern with Dr. Judi Walton when she was first

Contents Retrieved from Microscopy Listserver Archives
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I was a student intern with Dr. Judi Walton when she was first using lead
aspartate in 1977. I don't remember if we used it with tissue, but I know
we used it with cell culture monolayer samples for TEM. We used it without
poststaining. She developed this as Walton's Lead Aspartate. I don't know
if it is still on the market....

Barbara L. Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster Rm 632
San Francisco, CA 94115
email: Bplowman-at-sf.uop.edu




From daemon Tue Mar 27 18:35:48 2001



From: William P. Sharp :      wsharp-at-asu.edu
Date: Tue, 27 Mar 2001 16:33:21 -0700
Subject: 3D TEM Reconstruction Software Summary

Contents Retrieved from Microscopy Listserver Archives
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Dear List:

Thank you for the answers to my inquiry regarding 3D reconstruction of TEM
serial sections and / or EM tomography. They were very useful and we have a
much better idea of how to proceed with our project. I have, at the request
of a couple of list subscribers, summarized the answers in a Word file and
sent the file to them. It is available to anyone who emails me and asks -
it is kind of long, but there is good advice and contact information
included for anyone who is beginning in this area.

Again - Thanks to all who helped. I wouldn't know how to cover the bases
for those who depend on me without this list! Thanks to Nestor, too!

Regards,
Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Tue Mar 27 19:42:46 2001



From: Sean Ward :      sean-at-physio.unr.edu
Date: Tue, 27 Mar 2001 17:39:44 -0800
Subject: LM - Problem with oil in my objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Readers,
I have a Nikon 63x oil objective that is used on an inverted scope. It is a
multi user facility and I just noticed today that oil has seeped into the
objective making it almost useless to use.

(i) Should I tackle the job of cleaning this lens myself or (ii) Does anyone
know of a facility that would clean this lens at a reasonable price?

I used to have silicon around the top of the lens to prevent this problem
but it came off and I forgot to replace it.

Thanks for any information on this topic.


Sean M. Ward
Associate Professor
Department of Physiology and Cell Biology
University of Nevada School of Medicine
Manville Medical Sciences Building
Reno NV 89557
Tel: (775) 784-6061
Fax:(775) 784-6903




From daemon Tue Mar 27 23:12:09 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Wed, 28 Mar 2001 17:04:06 +1300
Subject: Visualizing Mineral Oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been asked to look at the microscopic distribution of a mineral
oil used in plant sprays on a plant surface. The spray is dispersed
in water at 1-2% so the final volumes on the surface can be quite
low. Most of the techniques I have seen are concerned with the
total distribution of the spray and use either fine particles or dyes in
the aqueous phase and do not track the oil. I have had only limited
success with the natural autofluorescence of the oil,
cathodoluminescence in the SEM and with common chromatic
dyes such as oil red. Visualization is just possible if we deposit
pure oil on the surface but not the suspension. Has anyone any
suggestions??

Thanks in advance

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


From daemon Wed Mar 28 00:07:05 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Wed, 28 Mar 2001 00:03:35 -0600
Subject: RE: measuring microscope stage movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, an interferometer could be used to measure the stage movement, but
it's an expensive option. If you truly need 1 micron placement, there are
few methods available. If you can accept somewhat less resolution, a
machinist's dial micrometer with a stand can give you 1/10,000 of an inch
measurement rather inexpensively, but with limited travel. You didn't
mention the overall length of your samples.

Being based at a university, try your local campus machine shop.
Machinists tend to covet those precision instruments, but perhaps you
could borrow one for a short time (just don't sign in blood).

On Tuesday, March 27, 2001 2:33 PM, Tobias Baskin
[SMTP:BaskinT-at-missouri.edu] wrote:
}
} Greetings,
} I am taking overlapping video images of a growing root
} through a horizontal light microscope. For analysis, I need to put
} the images back in register. I have tried incorportating various high
} contrasty things into the background but this introduces various
} problems ranging from death to the root to requiring a large overlap
} between successive images. As an alternative, I thought of measuring
} the displacement of the stage. A typical displacement between images
} is around 300 microns and I need to measure this displacement to
} within around 1 micron. I only need to work in one dimension, which
} can be parallel to one of the edges of the stage. I have in mind
} something like a "giant" afm detection set up, with a laser bouncing
} off the edege of stage and picked up by a detector. But this is just
} wild imagination on my part. Anyone out there have any *knowledge*
} about how this might be accomplished?
}
} Thanks in advance,
} Tobias
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109 Tucker Hall
} / / / / \ \ \ Biological
Sciences
} /_ / __ /__ \ \ \__ University of
Missouri
} / / / \ \ \ Columbia, MO
USA
} / / / \ \ \ 65211-7400
} / / ___ / \ \__/ \ ____ voice: 573-882-0173
} fax: 573-882-0123
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Wed Mar 28 00:13:18 2001



From: Dr.Manfred Rohde :      mro-at-gbf.de
Date: Mi, 28 Mrz 2001 08:10:47 +0100
Subject: FESEM of bacteriophages

Contents Retrieved from Microscopy Listserver Archives
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Dear collegues
has anyone out there has any experiences with imaging bacteriophages in a
FESEM and does anyone know of some published pictures.
Thank's in advance. Manfred



From daemon Wed Mar 28 01:43:33 2001



From: Uta Klement :      klement-at-em.chalmers.se
Date: Wed, 28 Mar 2001 12:15:22 +0200
Subject: PhD-thesis announcement (TEM)

Contents Retrieved from Microscopy Listserver Archives
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Ian
I have never tried this, but one possibility would be to add a
fluorochrome to the system. Nile red is an oil-soluble fluorochrome,
only sparingly soluble in aqueous phase, strongly fluorescent only
when it partitions into an apolar environment but non-fluorescent in
aqueous phase. It should be possible to image Nile-red labelled oil
droplet footprints by fluorescence LM with FITC-type filter systems
and possibly also by cathodoluminescence in SEM. If you are working on
leaves the red autofluorescence of the chloroplasts should be filter
out with a green barrier filter. The Leica GFP Plant filter sets may
therefore be suitable.
see Greenspan, P., Mayer,E.P. and Fowler,S.D. (1985) Nile red: a
selective fluorescent stain for intracellular lipid droplets. Journal
of Cell Biology 100, p965-973.

Hope this helps
Chris
----- Original Message -----
} From: "IAN HALLETT" {ihallett-at-hortresearch.co.nz}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 28, 2001 5:04 AM


1 micron is a pretty demanding tolerance. Do you really need it to be
this good?
Stages used to be provided with x and y vernier scales, but today a
stepper motor stage drive is often used controlled by a computer
which counts the steps.
Mitutoyo make electronic measuring devices accurate to about 5 or 10
microns which are used in electronic calipers. Similar modules are
available for mounting on engineering x,y stages, for example of
milling machines. Electronic supplies companies like Farnell, RS
Components and others supply these.

----- Original Message -----
} From: "Tobias Baskin" {BaskinT-at-missouri.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, March 27, 2001 9:33 PM




The Department of Engineering Metals, Chalmers University of Technology
(Göteborg, Sweden) is seeking:

DOCTORAL STUDENT
Microstructure and Chemistry of Nanostructured Materials using Transmission
Electron Microscopy

Any technological application of nanostructured materials is only possible
if their properties and their microstructure are well understood so that
they can be manufactured reproducibly. Concentrating on Ni- and Co-based
materials analytical transmission electron microscopy will be applied to
characterize the microstructure of the material as well as to monitor the
microstructural evolution upon heating. The work will be performed in close
collaboration with colleagues at the University of Toronto, Canada. The
experiments will be carried out on a Zeiss 912 Omega with inbuilt imaging
energy filter, however, also a Philips CM 200 FEG equipped with EDS and
Gatan imaging filter (GIF) is accessible. A special heating holder is
available to perform the in-situ heating experiments.

The appointment applies to full time for a maximum period of five years. In
addition to the research activities departmental duties, i.e. a limited
amount of educational, research and/or administrative work up to 20% of full
time has to be performed. The initial monthly salary is SEK 18.000, however
depending on the academic degree. The applicant must have an academic degree
(MSc, BSc or equivalent) in materials science/materials engineering, physics
or a similar subject area. Experience in (analytical) transmission electron
microscopy is a merit. The position is available immediately.

For further information about the position, please contact:
Prof. Uta Klement
Chalmers University of Technology, Department of Engineering Metals
Tel: +46 31-772 1264, Fax: +46 31-772 1262
E-mail: Klement-at-em.chalmers.se

A written application including the reference number 23/2001, with
(attested) personal record, brief description of research interests, name
and e-mail addresses to three reference persons, possible day for taking
possession of the position, and the documents that you want to refer to
should have arrived at the Registrar, Chalmers University of Technology,
SE-412 96 Göteborg, Sweden no later than April 27, 2001.




From daemon Wed Mar 28 04:34:26 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 28 Mar 2001 11:27:28 +0100
Subject: Re: measureing micrscope stage movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tobias

I can't visualize exactly how you are doing this, but I assume you are
using reflected rather than transmitted light.

In the dim and distant past I have used a 'traveling microscope' which
consists of a lens system with a cross-hair which can be moved along a
metal rail (at least 200mms long). The rail had divisions marked off
accurately in mms and a sliding Vernier scale so I would guess that it
would be accurate to 10um or maybe better. I suppose that it may be
possible to attach a camera system instead of the lens but it would be
best to try and borrow one to find out. I assume that a stage micrometre
(slide marked off in fractions of 1mm or 10mm) could not be adapted to
your purposes but it might be a useful way of calibrating any system you
adopt.

Malcolm

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Tobias Baskin wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Greetings,
} I am taking overlapping video images of a growing root
} through a horizontal light microscope. For analysis, I need to put
} the images back in register. I have tried incorportating various high
} contrasty things into the background but this introduces various
} problems ranging from death to the root to requiring a large overlap
} between successive images. As an alternative, I thought of measuring
} the displacement of the stage. A typical displacement between images
} is around 300 microns and I need to measure this displacement to
} within around 1 micron. I only need to work in one dimension, which
} can be parallel to one of the edges of the stage. I have in mind
} something like a "giant" afm detection set up, with a laser bouncing
} off the edege of stage and picked up by a detector. But this is just
} wild imagination on my part. Anyone out there have any *knowledge*
} about how this might be accomplished?
}
} Thanks in advance,
} Tobias
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109 Tucker Hall
} / / / / \ \ \ Biological Sciences
} /_ / __ /__ \ \ \__ University of Missouri
} / / / \ \ \ Columbia, MO USA
} / / / \ \ \ 65211-7400
} / / ___ / \ \__/ \ ____ voice: 573-882-0173
} fax: 573-882-0123


From daemon Wed Mar 28 05:21:13 2001



From: Feng Wu :      fwu-at-bgumail.bgu.ac.il
Date: Wed, 28 Mar 2001 13:13:37 +0200
Subject: adjust the intensity of the image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All,
I take some diffraction contrast pctures. As the thickness of the samples
changes sharply the brightness of the images is not uniform. Does someone know
any software to adjust the brightness for the whole image?
Thanks inadvance.
Best regards.
Feng
**********************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

voice 972-8-6461473

fwu-at-bgumail.bgu.ac.il
**********************************************




From daemon Wed Mar 28 05:30:33 2001



From: jshields-at-cb.uga.edu
Date: Wed, 28 Mar 2001 09:04:49 -0500
Subject: info on auto-montage by syncroscopy

Contents Retrieved from Microscopy Listserver Archives
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A good tool maker should be able to make you someting the will do an order
of magnitued beter than 1/10,0000 by using a standard micrometer head and
setting to drive a wedge at about 10 degrees. I don't remember the exact
angle but i used it to get .00001 on a lathe wiht 0001 devisions. I dont
expect you would get all the precision but you sould get .00022 to .00004

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "Allen R. Sampson" {ars-at-sem.com}
To: "'Tobias Baskin'" {BaskinT-at-missouri.edu} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 28, 2001 12:03 AM


Hi folks,
If anyone has experience with and opinions about the product Auto-
Montage by Syncroscopy, please contact me or Mark Farmer off-
listserve.
Thanks in advance,

John Shields
EM Lab
University of GA
Athens
jshields-at-cb.uga.edu

or Mark Farmer
farmer-at-cb.uga.edu



From daemon Wed Mar 28 08:03:48 2001



From: jshields-at-cb.uga.edu
Date: Wed, 28 Mar 2001 08:58:02 -0500
Subject: Re: LM - Problem with oil in my objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Sean,
We had the same problem with one of our objectives on the Nikon
inverted. The lens should not allow oil in if it is sealed correctly,
even without the "seal" you provided. Your lens is either older and
the seal is worn, or you've been cleaning the oil off with a harsh
solvent (I've seen some people using xylene regularly). Somewhere
in the back of my mind (a dusty and unreliable source) I think
silcon is not good for the seal. Another solution is to take finger
cots or make them by cutting the finger tips from gloves and pull
them down over the lens if you are still unsure of your seal.
We ended up sending the lens to Nikon for repair. I seem to
remember it cost around $200 or so to have them clean it and
replace the seal, but you can call to get a quote.
good luck


On 27 Mar 2001, at 17:39, Sean Ward wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Dear Readers,
} I have a Nikon 63x oil objective that is used on an inverted scope.
} It is a
} multi user facility and I just noticed today that oil has seeped into
} the objective making it almost useless to use.
}
} (i) Should I tackle the job of cleaning this lens myself or (ii) Does
} anyone know of a facility that would clean this lens at a reasonable
} price?
}
} I used to have silicon around the top of the lens to prevent this
} problem but it came off and I forgot to replace it.
}
} Thanks for any information on this topic.
}
}
} Sean M. Ward
} Associate Professor
} Department of Physiology and Cell Biology
} University of Nevada School of Medicine
} Manville Medical Sciences Building
} Reno NV 89557
} Tel: (775) 784-6061
} Fax:(775) 784-6903
}
}


John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu


From daemon Wed Mar 28 08:18:23 2001



From: Ramin Rahbari :      RRahbari-at-synapticcorp.com
Date: Wed, 28 Mar 2001 09:09:12 -0500
Subject: measureing micrscope stage movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tobias,
What you suggest may not be worth the hassle. Potential error introduced by
temp. variations on the stage would be problematic. Is it not possible to
include an internal standard, for example an embedded stage micrometer,
ruler, calibrated cover plate for the incubation vessel,...
Ramin

-----Original Message-----
} From: Tobias Baskin [mailto:BaskinT-at-missouri.edu]
Sent: Tuesday, March 27, 2001 3:33 PM
To: Microscopy-at-sparc5.microscopy.com


Greetings,
I am taking overlapping video images of a growing root
through a horizontal light microscope. For analysis, I need to put
the images back in register. I have tried incorportating various high
contrasty things into the background but this introduces various
problems ranging from death to the root to requiring a large overlap
between successive images. As an alternative, I thought of measuring
the displacement of the stage. A typical displacement between images
is around 300 microns and I need to measure this displacement to
within around 1 micron. I only need to work in one dimension, which
can be parallel to one of the edges of the stage. I have in mind
something like a "giant" afm detection set up, with a laser bouncing
off the edege of stage and picked up by a detector. But this is just
wild imagination on my part. Anyone out there have any *knowledge*
about how this might be accomplished?

Thanks in advance,
Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological
Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO
USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Wed Mar 28 09:13:20 2001



From: Smartech :      smartech-at-javanet.com
Date: Wed, 28 Mar 2001 10:17:49 -0500
Subject: EM, Why did they replicate surfaces w/ carbon?

Contents Retrieved from Microscopy Listserver Archives
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I have heard mention of replicating surfaces with carbon for analysis w/ EM,
it seems like it may be history now, but I was wondering if someone could
"clue me in" with when it was required.

Thanks

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Mar 28 09:18:30 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 28 Mar 2001 10:19:33 -0500
Subject: Re: LM - Problem with oil in my objective

Contents Retrieved from Microscopy Listserver Archives
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At 5:39 PM -0800 3/27/01, Sean Ward wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

You have my deepest sympathies! We had the same experience with a
Zeiss lens on a multi-user Axiovert a few years ago.
I would NOT recommend trying this on your own. High power lenses are
complexes of many lens elements and, in my humble opinion, should
only be disassembled by those who know what they are doing. We ended
up sending our lens back to Germany. 6 weeks and many $$$ later, it
was like new.

Ask you Nikon dealer what s/he would suggest.

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Mar 28 10:16:36 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Wed, 28 Mar 2001 09:11:00 -0700 (MST)
Subject: Re: LM - Problem with oil in my objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is this correct? There isn't such a tight seal at the telescoping part of
the objective barrel - this (in my experience) is where oil can get in
unless a lens condom is used - or have I been woefully misled?

Tamara

On Wed, 28 Mar 2001 jshields-at-cb.uga.edu-at-sparc5.microscopy.com wrote:

}
} Hi Sean,
} We had the same problem with one of our objectives on the Nikon
} inverted. The lens should not allow oil in if it is sealed correctly,
} even without the "seal" you provided. Your lens is either older and
} the seal is worn, or you've been cleaning the oil off with a harsh
} solvent (I've seen some people using xylene regularly). Somewhere
} in the back of my mind (a dusty and unreliable source) I think
} silcon is not good for the seal. Another solution is to take finger
} cots or make them by cutting the finger tips from gloves and pull
} them down over the lens if you are still unsure of your seal.
} We ended up sending the lens to Nikon for repair. I seem to
} remember it cost around $200 or so to have them clean it and
} replace the seal, but you can call to get a quote.
} good luck
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Wed Mar 28 14:58:30 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Wed, 28 Mar 2001 12:50:02 -0800
Subject: TEM: Double-tilt sample holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi everybody,

I am looking to purchase a used double-tilt sample holder for a 200CX JEOL
TEM. Does anyone know of any that is available for sale? Thank you.

Thearith Ung



From daemon Wed Mar 28 15:45:58 2001



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 28 Mar 2001 15:56:25 -0600
Subject: ImunoEM

Contents Retrieved from Microscopy Listserver Archives
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Can anyone recommend a good source for an antibody to BrdU for em. Also, can you recommend a fixation and embedding protocol?

TIA

Hank Adams
Manager
Integrated Microscopy Core
Department of Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx


From daemon Wed Mar 28 16:47:34 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 28 Mar 2001 17:46:57 -0400
Subject: RE: Meas Stage Movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might try an ordinary dial gauge, a device widely used for measuring
small movements in machine shops. Check with your friendly machinist, or
try your local hardware store. Dial gauges will easily measure 0.0005"
(0.01 mm), and are simple, inexpensive, and easy to use. I am sure you can
order one from Small Parts Co. I don't happen to have their address here,
but you can probably find them on the internet.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Mar 28 17:11:31 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 28 Mar 2001 18:11:15 -0400
Subject: RE: Carbon replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Prior to the introduction of techniques for preparing and examining thin
metal specimens by transmission electron microscopy, which came into
general use in the late 1950s, virtually all electron microscopic studies
of non-biological materials was done by examining exposed surfaces (treated
in various ways to reveal the characteristics of the internal structure)
using replical techniques. Early on, these replicas were made of various
polymeric materials (e.g. formvar, colloidion, etc.). There were various
shortcomings of these replicas, mainly their resolution was limited to
something of the order of 50 Angstroms. In 1954 D. E. Bradley introduced a
convenient method for depositing very thin films of carbon. These films
were proven to be much better than the polymer films for producing surface
replicas. They were stronger, they were electrically conductive, they could
be made much thinner, but most of all could provide resolution below 20
Angstroms. Therefore they quickly became the most widely used type of
replica film. You can find a rather complete discussion of the many
replica techniques that were in use back then in the book 'Techniques for
Electron Microscopy', D. E. Kay, Editor, Blackwell Scientific Pub., 1965.
These techniques are hardly ever mentioned now-a-days, because there are so
many newer techniques now available for producing thin specimens for direct
examination.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Mar 28 17:38:17 2001



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Wed, 28 Mar 2001 17:31:16 -0800
Subject: inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I received this inquiry recently. Can anybody help this person?
Hi.
I am a biology student and I have to do an assignment on electron or
light
microscopy an it's impact on society. For example, in forensic science
or
medical research.
If you have any information that would help with this, me please email
it to
me as soon as possible.
It would be greatly appreciated.
Thankyou,
Nicole.

Nicole Forzan {phindola16-at-hotmail.com}
Thanks,
Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Wed Mar 28 18:43:35 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 28 Mar 2001 18:41:59 -0600
Subject: Re: EM, Why did they replicate surfaces w/ carbon?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Date: Wed, 28 Mar 2001 13:20:14 -0800
} To: "Smartech" {smartech-at-javanet.com}
} From: Mary Mager {mager-at-interchange.ubc.ca}
} Subject: Re: EM, Why did they replicate surfaces w/ carbon?
} Cc: Microscopy
}
} Dear Ric,
} We routinely do carbon replicas of steel surfaces to study the very fine
precipitates by TEM, free of the steel matrix. The method is: etch the steel
lightly, carbon coat in the evaporator, score the carbon coat into 3 mm.
squares with a razor blade, place the steel in the etchant until the carbon
squares float off. Scoop up the floating carbon squares with a TEM grid.
} For SEM of surfaces that are too big to go into the SEM chamber or that the
cops won't let you take away, a cellulose acetate replica is used. It also
removes surface material, allowing you to analyse it free of the matrix and
cleaning the matrix. It is often used for fractures. The cellulose acetate
film is softened in acetone and the sample surface is wetted with acetone.
The acetate is placed on the acetone-wetted surface and left for about 0.5
hour to solidify. It conforms exactly to the surface, so you can study the
fracture surface without putting the sample in the SEM, after coating the
acetate piece to make it conductive.
} That is very brief, you can contact me for more information.
} At 10:17 AM 3/28/01 -0500, you wrote:
}
} } I have heard mention of replicating surfaces with carbon for analysis w/ EM,
} } it seems like it may be history now, but I was wondering if someone could
} } "clue me in" with when it was required.
} }
} } Thanks
} }
} } Ric
} }
} } SMARTech
} } 860-491-3299
} } www.semguy.com
} } 19 Cornwall Drive
} } Goshen CT 06756
} }
} Regards,
} Mary
}
}
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Mar 28 20:06:07 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 29 Mar 2001 14:07:04 GMT+1200
Subject: Mains supply in Sweden

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi

Can someone tell me what the mains supply voltage is in Sweden?

thanks

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Mar 29 00:12:04 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 29 Mar 2001 00:06:18 -0600
Subject: Re: Meas Stage Movement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Wil Bigelow" {bigelow-at-engin.umich.edu}
} You might try an ordinary dial gauge, a device widely used for measuring
} small movements in machine shops. Check with your friendly machinist,
or
} try your local hardware store. Dial gauges will easily measure 0.0005"
} (0.01 mm), and are simple, inexpensive, and easy to use. I am sure you
can
} order one from Small Parts Co. I don't happen to have their address
here,
} but you can probably find them on the internet.
}
Wil,

Thanks for mentioning this it solves a problem I have on how to tell how
much I move the stage for some experments I am doing trying to enhance the
quality of video images by stacking multiple images. I need to get a
little shift of the view for better averageing. I never thought using the
test indicator laying 6 feet from the scope.

Looking through my machine tool catalog what you want if you go this route
is called a test indicator. It is an order of magnitude more accrute than
most dial indicators. They can be purchased with calimed accurcies down to
0.00012 or .003 mm. They are avalible in either mecanical dial or digital
and range in cost from $100 to $500USD. They are built to be used in
machine shops and should give very long service in a labrotory. The
accurcy ranges from .001 to .00012 over the price range.

A lever arangement could be built to extend the resolution. It would
require careful work to retain the accurcy.

If you need help in finding a vendor or how one would work I have spent
countless hours using one.

good luck
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00






From daemon Thu Mar 29 07:53:45 2001



From: donald j marshall :      dmrelion-at-world.std.com
Date: Thu, 29 Mar 2001 08:47:58 -0500 (EST)
Subject: Re: Mains supply in Sweden

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ritch,


Panel components, Inc. info-at-panelcomponents.com has a great
catalog with info on power,plugs, receptacles, etc. for most countries.

I have no $ interest...... but have purchased items from them.



Don Marshall



} From Microscopy-request-at-sparc5.microscopy.com Wed Mar 28 21:07:09 2001
From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} Organization: Dept of Geology, Univ of Auckland
} To: Microscopy-at-sparc5.microscopy.com
} Date: Thu, 29 Mar 2001 14:07:04 GMT+1200


} Hi
}
} Can someone tell me what the mains supply voltage is in Sweden?
}
} thanks
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)


From daemon Thu Mar 29 11:08:29 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 29 Mar 2001 10:57:57 -0600
Subject: agarose - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Thanks to all those who responded to my question regarding enrobing of cells in
agarose.
Bruce
Bruce Cutler
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas





From daemon Thu Mar 29 12:25:07 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 29 Mar 2001 13:19:57 -0500
Subject: Re: Mains supply in Sweden

Contents Retrieved from Microscopy Listserver Archives
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Thu, 29 Mar 2001 13:19:58 -0500 (EST)
User-Agent: Microsoft-Entourage/9.0.2509


Let me actually try and answer your question.

In keeping with most of the rest of Europe I think it is 220/240.

On 3/29/01 8:47 AM, "donald j marshall" {dmrelion-at-world.std.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} ritch,
}
}
} Panel components, Inc. info-at-panelcomponents.com has a great
} catalog with info on power,plugs, receptacles, etc. for most countries.
}
} I have no $ interest...... but have purchased items from them.
}
}
}
} Don Marshall
}
}
}
} } From Microscopy-request-at-sparc5.microscopy.com Wed Mar 28 21:07:09 2001
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} } Organization: Dept of Geology, Univ of Auckland
} } To: Microscopy-at-sparc5.microscopy.com
} } Date: Thu, 29 Mar 2001 14:07:04 GMT+1200
}
}
} } Hi
} }
} } Can someone tell me what the mains supply voltage is in Sweden?
} }
} } thanks
} }
} } rtch
} }
} } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } Department of Geology Fax : 64 9 3737435
} } The University of Auckland email : r.sims-at-auckland.ac.nz
} } Private Bag 92019
} } Auckland
} } New Zealand
} }
}
} Donald J. Marshall
} Relion Industries
} P.O. Box 12
} Bedford, MA 01730
} Ph: 781-275-4695
} FAX: 781-271-0252
} email dmrelion-at-world.std.com
}
} Cathodoluminescence, mass spectroscopy, electron beam technology
}
}
} "A weed is a flower out of place."
}
} (Please note: Do not send email with attachments to this address. Instead,
} send it to dmrelion-at-aol.com. Thank you.)
}
}

--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"




From daemon Thu Mar 29 13:43:42 2001



From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 29 Mar 2001 21:35:47 +0200
Subject: Re: Mains supply in Sweden

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From
http://www.sverigeturism.se/smorgasbord/smorgasbord/service/electricity.html:

The voltage is 230V in Sweden. All new buildings
erected from January 1, 1994, will be in accord with EU
standards. That is, all sockets are earthed. In every
public milieu there will only be earthed sockets. In all
other buildings erected before the date of January 1,
1994, earthed sockets will be found in bathrooms, as
well as in kitchens. Otherwise there are simple sockets.
In case one needs an adapter it can be acquired in an
electricity store. There are no transformers for 130 volt
to 230 volt to be found.


"John F. Mansfield" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Let me actually try and answer your question.
}
} In keeping with most of the rest of Europe I think it is 220/240.
}
} On 3/29/01 8:47 AM, "donald j marshall" {dmrelion-at-world.std.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } ritch,
} }
} }
} } Panel components, Inc. info-at-panelcomponents.com has a great
} } catalog with info on power,plugs, receptacles, etc. for most countries.
} }
} } I have no $ interest...... but have purchased items from them.
} }
} }
} }
} } Don Marshall
} }
} }
} }
} } } From Microscopy-request-at-sparc5.microscopy.com Wed Mar 28 21:07:09 2001
} } From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} } } Organization: Dept of Geology, Univ of Auckland
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Date: Thu, 29 Mar 2001 14:07:04 GMT+1200
} }
} }
} } } Hi
} } }
} } } Can someone tell me what the mains supply voltage is in Sweden?
} } }
} } } thanks
} } }
} } } rtch
} } }
} } } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } } Department of Geology Fax : 64 9 3737435
} } } The University of Auckland email : r.sims-at-auckland.ac.nz
} } } Private Bag 92019
} } } Auckland
} } } New Zealand
} } }
} }
} } Donald J. Marshall
} } Relion Industries
} } P.O. Box 12
} } Bedford, MA 01730
} } Ph: 781-275-4695
} } FAX: 781-271-0252
} } email dmrelion-at-world.std.com
} }
} } Cathodoluminescence, mass spectroscopy, electron beam technology
} }
} }
} } "A weed is a flower out of place."
} }
} } (Please note: Do not send email with attachments to this address. Instead,
} } send it to dmrelion-at-aol.com. Thank you.)
} }
} }
}
} --
}
} Dr. John Mansfield CPhys MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282
} Cellular Phone: (734) 358-7555
} (Leaving a phone message at 936-3352 is preferable to 358-7555)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42° 16' 48" Long. 83° 43' 48"

--
Henrik Kaker, Ph.D.
Metal Ravne d.o.o.
SEM-EDS Lab
Koroska cesta 14, 2390 Ravne
Slovenia
Phone: +386 602 21 131
Fax: +386 602 20 436
http://www.kaker.com




From daemon Thu Mar 29 14:03:46 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Thu, 29 Mar 2001 14:58:02 -0500
Subject: 3 D COMPUTER PROGRAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

I would like to know what computer programs are out there for taking serial
sections and creating a 3 D image. Can any one help? Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Thu Mar 29 18:59:34 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 29 Mar 2001 19:58:14 -0500
Subject: Immunogold WS Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html}
{font face="Times, Times"} Dear Listers, {br}
{br}
Immunogold Workshop Anouncement {br}
{br}
The Electron Microscopy Facility for the Life Sciences, a shared
technology laboratory in the Life Sciences Consortium at Penn State
University is hosting a three-day workshop on immunogold techniques from
May 21-23, 2001. Dr. Jan Luenissen from the Aurion Immunogold Reagent
& {br}
Accessories, an internationally known expert in the field, will be the
instructor for the workshop. The workshop will include lectures, hands-on
training, round table discussions, and presentations on
applications.  Also, participants of the workshop will be able to
work on their own samples during the workshop.  The following is the
workshop main curriculum.  If you are interested in attending or
need more information about the workshop, please contact the workshop
technical coordinator Hong Yi by phone (404-727-8692) or email
(hyi-at-emory.edu). {br}
{br}
{br}
MAIN CURRICULUM {br}
{br}
The properties of gold particles and their protein conjugates. {br}
Theories underlying immunogold labeling protocols. {br}
Silver enhancement of gold particles {br}
Immunogold labeling on a variety of sample preparations for LM. {br}
Immunogold labeling for EM {br}
a. Pre-embedding immunogold labeling using ultrasmall gold conjugates
and silver enhancement. {br}
b. Post-embedding immunogold labeling using conventional colloidal gold
conjugates and ultrasmalll gold conjugates. {br}
Pre- and post-embedding double immunogold labeling. {br}
Background minimization in immunogold labeling {br}
Signal amplification in immunogold labeling. {br}
{br}
{br}
Rosemary Walsh, Manager {br}
The Electron Microscope Facility for the Life Sciences {br}
A Shared Technology in The Life Sciences Consortium {br}
1 South Frear Lab {br}
Penn State University {br}
University Park, PA    16802 {br}
(815) 865-0212 {br}
{a href="http://www.lsc.psu.edu/stf/em/home.html" eudora="autourl"} http://www.lsc.psu.edu/stf/em/home.html {/a} {br}
  {br}
{br}
{br}
{br}
{/font} {/html}



From daemon Thu Mar 29 22:47:06 2001



From: mcmouldk-at-ust.hk
Date: Fri, 30 Mar 2001 12:41:42 +0800
Subject: TEM Second-hand

Contents Retrieved from Microscopy Listserver Archives
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Greetings,

I have been asked what would the market value of a 10 year old JEOL 2000FX
TEM would be. The TEM is in fully working order. Any ideas?

Is there a market for second-hand TEMs?

Many thanks

Keith.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Fri Mar 30 03:23:38 2001



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Thu, 29 Mar 2001 14:58:02 -0500
Subject: 3 D COMPUTER PROGRAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tim,

There exists a homepage of 3-Dimensional Microscopy Labs
(http://3dem.sdsc.edu/) in which are listed various packages for 3D-EM
Image Analysis (including reconstruction of serial secions).
Additionally a mailing list exists.

BTW: A nice animation can be found in a Web-Page of the University of
Utrecht (The Netherlands):
http://emsaserv.bio.uu.nl/3dem/ANIMATED_INTRODUCTION/animated_introduction_1.html

I hope this helps.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Herbststrasse 7
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: http://www.tvips.com/
Email: ingo.daberkow-at-tvips.com



-------- Original Message --------


Dear Listers:

I would like to know what computer programs are out there for taking
serial
sections and creating a 3 D image. Can any one help? Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu


From daemon Fri Mar 30 04:39:18 2001



From: Prof. Luisa Dusonchet :      dusonc-at-unipa.it
Date: Fri, 30 Mar 2001 12:40:55 +0200
Subject: TEM autoradiography

Contents Retrieved from Microscopy Listserver Archives
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Dear collegues,
I need to perform an autoradiographic study in electron microscopy. From
some papers in the literature it seems usuful to employ a phenidon
developer and a gold latensification solution; since I did not find any
commercial product available, I need to prepare it in my lab. I would be
very grateful to you if you can provide me with some information about
the composition and detailed method of preparation and storage of the
different solutions. Thank you very much.
Maria Meli



From daemon Fri Mar 30 10:02:25 2001



From: Doug Cromey :      Cromey-at-Arizona.edu
Date: Fri, 30 Mar 2001 08:54:08 -0700
Subject: Web site announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I am pleased to announce the completely revised and updated edition of
"Microscopy & Imaging Resources on the WWW" is now available at a new
URL. The goal of this site has always been to provide resources for
University students, staff and faculty who want to learn more about
microscopy and imaging. The site includes K-12 educational links,
information on Light Microscopy, Histology, Confocal Microscopy,
Fluorescence Techniques, Electron Microscopy, and Digital Imaging.

http://swehsc.pharmacy.arizona.edu/exppath/

"Microscopy & Imaging Resources on the WWW" is an outreach of the Southwest
Environmental Health Sciences Center, which is funded by the National
Institute of Environmental Health Sciences (NIEHS is one of the National
Institutes of Health, part of the U.S. Department of Health and Human
Services).

Yours,
Doug Cromey
Manager, Experimental Pathology Core, SWEHSC
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (NEW email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Fri Mar 30 10:54:11 2001



From: Steven Celotto :      s.celotto-at-liverpool.ac.uk
Date: Fri, 30 Mar 2001 17:50:13 +0100
Subject: Fraser fonts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listerservers,
I have heard that there are a set of fonts called 'Fraser fonts' with
over bars etc. that are useful for indices.
Does it exist.
Where can such a font be obtained?

Thanks in advance,
Steven


--
Dr. Steven Celotto
Department of Engineering
Materials Science & Engineering
University of Liverpool
Brownlow Hill
Liverpool L69 3BX
UNITED KINGDOM

Telephone (UK) 0151-794-4692 (Int.) +44-151-794-4692
Fax. (UK) 0151-794-4675 (Int.) +44-151-794-4675
email: s.celotto-at-liv.ac.uk




From daemon Fri Mar 30 12:35:26 2001



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Fri, 30 Mar 2001 14:25:30 -0400
Subject: Update on CCTV system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

Thanks very much to all who have responded by mail or by phone to my
posting about the TEM CCTV system. I will be getting back to you early
next week.

Many people have offered to give the system a new home, so please, no more
inquiries.

Regards,
Bev Maleeff
GlaxoSmithKline Pharmaceuticals



From daemon Fri Mar 30 13:24:13 2001



From: JHoffpa464-at-aol.com
Date: Fri, 30 Mar 2001 14:20:03 EST
Subject: renal Em

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--part1_36.13be76bc.27f63663_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

ok taking a little survey. i am in a diagnostic EM lab. we mount out sections
on formvar coated slotted grids, so he can shoot pics of the whole glomerlus.
ok my question. how may of you out there doing diagnostis EM on renals do
this?
john

--part1_36.13be76bc.27f63663_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} ok taking a little survey. i am in a diagnostic EM lab. we mount out sections
{BR} on formvar coated slotted grids, so he can shoot pics of the whole glomerlus.
{BR} ok my question. how may of you out there doing diagnostis EM on renals do
{BR} this?
{BR} john {/FONT} {/HTML}

--part1_36.13be76bc.27f63663_boundary--


From daemon Fri Mar 30 14:14:27 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Fri, 30 Mar 2001 14:54:48 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




David Ziegler
U.S. Army, SBCCOM
AMSSB-RSS-MS(N)
Materials Science Team, SS&T
Natick, MA 01760-5020
TEL: (508) 233-6484
FAX: (508) 233-5521
Email: David.Ziegler-at-Natick.Army.Mil



From daemon Fri Mar 30 14:57:08 2001



From: Rishi Raj :      Rishi.Raj-at-Colorado.edu
Date: Fri, 30 Mar 2001 13:52:52 -0700
Subject: Information on the manufacturer of an SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have just acquired a used microscope made by ISI Inc. (their model
alpha - 1980). Would anyone please have information on how we can obtain
spare parts, filaments etc., for this machine. Many thanks for your
help...



From daemon Fri Mar 30 15:28:51 2001



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Fri, 30 Mar 2001 13:23:03 -0800
Subject: Re: renal Em

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John: I did renal biopsies by E-M for 12 years and knew the other two people in town who were also doing diagnostic E-M of renal biopsies. We all used grids with grid bars ( I always used naked grids). I chose grids with slender bars for all my work to maximize viewing without using slotted grids. We did not view "whole" glomeruli slices (quite low power I would think). I don't feel diagnoses were compromised versus using slotted grids. Since several sections are mounted on the 200 mesh grids, one should be able to view areas of interest just fine.

} } } {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com} 03/30 11:20 AM } } }
ok taking a little survey. i am in a diagnostic EM lab. we mount out sections
on formvar coated slotted grids, so he can shoot pics of the whole glomerlus.
ok my question. how may of you out there doing diagnostis EM on renals do
this?
john



From daemon Fri Mar 30 21:56:42 2001



From: COURYHOUSE-at-aol.com
Date: Fri, 30 Mar 2001 22:50:30 EST
Subject: Re: Web site announcement

Contents Retrieved from Microscopy Listserver Archives
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--part1_8b.484980e.27f6ae06_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

will add this link to the upcoming SMECC website.
thanks Ed Sharpe


} Subj: Web site announcement
} Date: 3/30/01 11:16:39 AM US Mountain Standard Time
} From: Cromey-at-Arizona.edu (Doug Cromey)
} To: microscopy-at-sparc5.microscopy.com, confocal-at-listserv.acsu.buffalo.edu
}
}
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
}
} I am pleased to announce the completely revised and updated edition of
} "Microscopy & Imaging Resources on the WWW" is now available at a new
} URL. The goal of this site has always been to provide resources for
} University students, staff and faculty who want to learn more about
} microscopy and imaging. The site includes K-12 educational links,
} information on Light Microscopy, Histology, Confocal Microscopy,
} Fluorescence Techniques, Electron Microscopy, and Digital Imaging.
}
} http://swehsc.pharmacy.arizona.edu/exppath/
}
} "Microscopy & Imaging Resources on the WWW" is an outreach of the Southwest
} Environmental Health Sciences Center, which is funded by the National
} Institute of Environmental Health Sciences (NIEHS is one of the National
} Institutes of Health, part of the U.S. Department of Health and Human
} Services).
}
} Yours,
} Doug Cromey
} Manager, Experimental Pathology Core, SWEHSC
} ....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Research Specialist, Principal University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (NEW email: Cromey-at-Arizona.edu) :
} :...................................................................:
} http://swehsc.pharmacy.arizona.edu/exppath/
} Home of: "Microscopy and Imaging Resources on the WWW"
}
}
}
}
} ----------------------- Headers ---------
}



--part1_8b.484980e.27f6ae06_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} will add this link to the upcoming SMECC website.
{BR} thanks Ed Sharpe
{BR}
{BR}
{BR} {BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"} Subj: {B} Web site announcement {/B}
{BR} Date: 3/30/01 11:16:39 AM US Mountain Standard Time
{BR} {I} From:    Cromey-at-Arizona.edu (Doug Cromey)
{BR} To:    microscopy-at-sparc5.microscopy.com, confocal-at-listserv.acsu.buffalo.edu
{BR} {/I}
{BR}
{BR}
{BR}
{BR} ------------------------------------------------------------------------
{BR} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} -----------------------------------------------------------------------.
{BR}
{BR}
{BR} Dear Colleagues,
{BR}
{BR} I am pleased to announce the completely revised and updated edition of
{BR} "Microscopy & Imaging Resources on the WWW" is now available at a new
{BR} URL.  The goal of this site has always been to provide resources for
{BR} University students, staff and faculty who want to learn more about
{BR} microscopy and imaging.  The site includes K-12 educational links,
{BR} information on Light Microscopy, Histology, Confocal Microscopy,
{BR} Fluorescence Techniques, Electron Microscopy, and Digital Imaging.
{BR}
{BR} http://swehsc.pharmacy.arizona.edu/exppath/
{BR}
{BR} "Microscopy & Imaging Resources on the WWW" is an outreach of the Southwest
{BR} Environmental Health Sciences Center, which is funded by the National
{BR} Institute of Environmental Health Sciences (NIEHS is one of the National
{BR} Institutes of Health, part of the U.S. Department of Health and Human
{BR} Services).
{BR}
{BR} Yours,
{BR} Doug Cromey
{BR} Manager, Experimental Pathology Core, SWEHSC
{BR} ....................................................................
{BR} : Douglas W. Cromey, M.S.          Dept. of Cell Biology & Anatomy  :
{BR} : Research Specialist, Principal   University of Arizona            :
{BR} : (office:  AHSC 4212A)            P.O. Box 245044                  :
{BR} : (voice:  520-626-2824)           Tucson, AZ  85724-5044   USA     :
{BR} : (FAX:  520-626-2097)             (NEW email: Cromey-at-Arizona.edu)  :
{BR} :...................................................................:
{BR}        http://swehsc.pharmacy.arizona.edu/exppath/
{BR}    Home of: "Microscopy and Imaging Resources on the WWW"
{BR}
{BR}
{BR} {/XMP} {/FONT} {FONT COLOR="#0f0f0f" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0"}
{BR}
{BR} ----------------------- Headers ---------
{BR} {/BLOCKQUOTE}
{BR} {/FONT} {/FONT} {FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0"}
{BR} {/FONT} {/HTML}

--part1_8b.484980e.27f6ae06_boundary--


From daemon Sat Mar 31 05:16:29 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 31 Mar 2001 05:10:59 -0600
Subject: RE: Information on the manufacturer of an SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ISI became Topcon, and then dropped out of the American market directly
awhile ago. Any of the SEM supply houses can provide supplies or you can
address your requests to Aspex, which was R. J. Lee until recently. They
currently represent the Topcon lines in North America and can be found at
http://www.rjleeinst.com/. Confused yet? It gets better. Good luck
finding anything about ISI or Topcon products on their website. Try the
phone lines.

Frankly, I hate having to trace the providence of this and other companies
that give up well known brand names for reasons known only to them. ISI
made inroads in this country primarily a s a low-bidder, thus many
instruments were originally in government labs. Had they made some attempt
to produce a product for the middle of the pack they may have done better
and may still be a player.

I'm not saying that they didn't produce a decent instrument - I still have
many that perform quite well. But they cut their own throats by playing to
an audience that has suffered devastating budgetary cutbacks in an era when
other segments were willing to pay vastly more for instruments that offer
little basic operational improvements.

On Friday, March 30, 2001 2:53 PM, Rishi Raj [SMTP:Rishi.Raj-at-Colorado.edu]
wrote:
} ------------------------------------------------------------------------
}
} We have just acquired a used microscope made by ISI Inc. (their model
} alpha - 1980). Would anyone please have information on how we can obtain
} spare parts, filaments etc., for this machine. Many thanks for your
} help...
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sat Mar 31 07:36:31 2001



From: Rachel Spicer :      spicer-at-oeb.harvard.edu
Date: Sat, 31 Mar 2001 17:06:03 -0500
Subject: LM/FM: adherence of xylem tissue to slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use plain 200 mesh grids. Cut and stain 2-3 grids/ block so we have
additional tissue to view in case the area of interest is under the grid
bar.

Becky Garrison
Pathology Supervisor
Shands Jacksonville
904-244-62237

-----Original Message-----
} From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, March 30, 2001 2:20 PM
To: microscopy-at-sparc5.microscopy.com



-----Original Message-----
} From: Garrison, Becky
Sent: Saturday, March 31, 2001 8:33 AM
To: '"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com';
microscopy-at-sparc5.microscopy.com


John:
We do around 500 renal biopsies per year and all the sections are
mounted on 200 mesh uncoated copper grids. We have an 8 year old Hitachi
7100 and use 60kv. The majority of the glomerulus can be viewed with the
3-4 serial sections lying randomly across the grid bars. We do not need a
picture of the whole glomerulus, rather most pictures are between 3,000 and
10,000X.
Dr. Tibor Nadasdy is the renal pathologist and decided last year that
all our renal biopsies would be captured with the digital camera onto a
computer and sent up to him via a network to his computer. So, at the
present time we use very little EM film. He diagnoses each biopsy and
e-mails representative digitized images to the nephrologists.




Karen L. Jensen, M.S.
Project Manager & Associate Scientist
Electron Microscopy Research Core




-----Original Message-----
} From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
[mailto:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, March 30, 2001 2:20 PM
To: microscopy-at-sparc5.microscopy.com


Hello -

Has anyone out there tried to adhere xylem sections to pre-coated
microscope slides like Fisher Probe-On Plus slides? I've tried and had a
zero percent section retention. I can imagine that this is because of the
scarcity of live cells (and plasma membranes). I've tried slow air drying
and various temperatures on the slide warmer. The sections are 15 microns,
and are from fixed (buffered paraformaldehyde) but not embedded samples.
I'm about to try Poly-L-lysine and amino-acyl silane treated slides, but
I'm not too hopeful because they (at least Poly-L) rely on the same
positively-charged surface principle. I want to avoid gelatin or albumin
subbing because I'm treating sections with protease, and also want to
minimize background staining. Any tips would be greatly appreciated.

Rachel




******************************************
Rachel Spicer
Biological Laboratories 3119
Organismic and Evolutionary Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

(617) 496-3580 (phone)
(617) 496-5854 (fax)
spicer-at-oeb.harvard.edu
******************************************



From daemon Sat Mar 31 19:08:26 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 31 Mar 2001 20:03:17 -0500
Subject: Support for ISI SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rishi Raj wrote:
============================================================
We have just acquired a used microscope made by ISI Inc. (their model alpha
- 1980). Would anyone please have information on how we can obtain spare
parts, filaments etc., for this machine. Many thanks for your help...
============================================================
The business of the ISI SEM's is now being handled by

Aspex Instruments LLC
Formerly: RJ Lee Instruments Ltd.
175 Sheffield Drive
Delmont, PA 15626 USA
Tel: 1-724-468-5400
Fax: 1-724-468-0225
E-mail: pssales-at-rjleeinst.com

The former manager of the SEM operation when it was still ISI, Michael
McCarthy, is now with Aspex. Michael might be the single most knowledgeable
person in terms of spare parts for the column and vacuum system. Several of
the main suppliers of consumables, like SPI Supplies, also offer filaments,
new and retipped, apertures, and the other items of that nature you would be
needing to maintain the microscope.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Mon Apr 2 03:32:31 2001



From: electron microscope laboratory :      emlab-at-udsm.ac.tz
Date: Mon, 02 Apr 2001 11:17:07 +0300
Subject: scholarship querry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All!

I desperately want to be a materials researcher/Electron Microscopist.
We have a Newly established Electron Microscope Laboratory. We need
knowledge and skills.
Please assist for a scholarship/suport as we have no funds, nor courses of
the like at our University.

Please write directly to me for any help.

Thanks,

Maulid Kivambe
mkivambe-at-hotmail.com




From daemon Mon Apr 2 07:49:23 2001



From: Laura Hernandez :      lahernan-at-udec.cl
Date: Mon, 02 Apr 2001 08:41:40 -0400
Subject: JEOL EPMA - H-type spectrometers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everybody,
We have an JEOL JXA 8600 EPMA in our Institute, with three WDS
spectrometers. We are planning to buy an other one, and we where thinking
about the H-type x-ray spectrometer.

Is there anybody in the list who has experience with this kind of
spectrometers that could give me some information about them (their
performance in general and also comparatively to standard spectrometers,
etc.)?

Thanks


Laura Hernandez
Laura Hernandez
Laboratorio Microsonda Electronica
Instituto GEA
Universidad de Concepcion
Casilla 160C
Concepcion
CHILE

e-mail: lahernan-at-udec.cl
FAX: 56-41-242535
TELEFONO:56-41-204861




From daemon Mon Apr 2 09:38:29 2001



From: sghoshro-at-NMSU.Edu
Date: Mon, 2 Apr 2001 08:33:15 -0600 (MDT)
Subject: Re: LM/FM: adherence of xylem tissue to slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rachel,

Try using Vectabond treated slides. vectabond is available from vector
laboratories. We had quite good results with leaf, stem, root sections
sticking to these slides. Vector lab: 1-800-227-6666

Good luck,

Soumitra


I have no financial interest in Vector Laboratories.



On Sat, 31 Mar 2001, Rachel Spicer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello -
}
} Has anyone out there tried to adhere xylem sections to pre-coated
} microscope slides like Fisher Probe-On Plus slides? I've tried and had a
} zero percent section retention. I can imagine that this is because of the
} scarcity of live cells (and plasma membranes). I've tried slow air drying
} and various temperatures on the slide warmer. The sections are 15 microns,
} and are from fixed (buffered paraformaldehyde) but not embedded samples.
} I'm about to try Poly-L-lysine and amino-acyl silane treated slides, but
} I'm not too hopeful because they (at least Poly-L) rely on the same
} positively-charged surface principle. I want to avoid gelatin or albumin
} subbing because I'm treating sections with protease, and also want to
} minimize background staining. Any tips would be greatly appreciated.
}
} Rachel
}
}
}
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}



From daemon Mon Apr 2 10:46:52 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 2 Apr 2001 11:42:03 -0400
Subject: RE: adjust the intensity of the image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can do a brightness leveling image process with a number of a standard packages.

The procedure is simply to do a Gaussian blur that gives you the slowly varying component of the background and subtract that from your original image.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Feng Wu [mailto:fwu-at-bgumail.bgu.ac.il]
} Sent: Wednesday, March 28, 2001 6:14 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: adjust the intensity of the image
} Sensitivity: Confidential
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi, All,
} I take some diffraction contrast pctures. As the thickness of
} the samples
} changes sharply the brightness of the images is not uniform.
} Does someone know
} any software to adjust the brightness for the whole image?
} Thanks inadvance.
} Best regards.
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
}
} fwu-at-bgumail.bgu.ac.il
} **********************************************
}
}
}


From daemon Mon Apr 2 13:39:41 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Mon, 02 Apr 2001 11:33:33 -0700
Subject: cauliflower strucutures on aniline films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear listers,

I'm posting this message for a colleague; as always, I appreciate your help.
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
Richland, WA
(509) 372-0692

} My graduate student has made TEM images of our plasma polymerized
aniline
} films. The films seem to have a "cauliflower" structure that could
} probably be described as a fractal pattern. Have you ever made plasma
} polymerized aniline films and if so did you see a cauliflower
structure?
} Your comments would be helpful. Thanks.
} Pat
}
} Patrick D. Pedrow, pedrow-at-eecs.wsu.edu, www.eecs.wsu.edu/~pedrow





From daemon Mon Apr 2 14:17:56 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 02 Apr 2001 15:14:19 -0400
Subject: Re: GACH Resin/polyethylene-imine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tobias Baskin wrote:

} Greetings,
} I have found that polyethlyene-imine (PEI) is much stickier
} than poly-lysine. I have no idea about the resin you mentioned, but
} PEI is sticky stuff. It comes as a liquid. I make a 0.1% soloution in
} ddwater, which I freeze in aliquots. Then I keep a working one in the
} fridge. I coat coverslips in the stuff by floating them on a drop of
} the PEI solution for about 10 sec, and then blotting off the excess
} and letting them air dry. In that conditions, the coated 'slips are
} good for at least months.
}
} Hope this helps,
} Tobias Baskin
}

Tobias:

Would this be Sigma catalogue number P-3143?

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Mon Apr 2 16:23:34 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 2 Apr 2001 17:17:54 -0400
Subject: Re: X-ray radiation detector for TEM's

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I am looking for a X-ray radiation detector used on TEMs. Currently we have
a Geiger counter that is only sensitive to low energy X-ray. The purpose of
the new detector is to sense high energy X-ray leakage from a 200 kV scope.

Any suggestion or clue will be greatly appreciated. Please contact off-line.


Dear Haifeng,
The x-ray monitors on our HVEM are wrapped in a metalic sheath so they will
be sensitive to the brehmsstrahlung spectrum of 1.2 MeV electrons. I don't
think that they could be easily connected to your scope, but you might look into
making a similar sheath for your existing Geiger counter. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Mon Apr 2 17:38:28 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 2 Apr 2001 17:33:37 -0500
Subject: JEOL EPMA - H-type spectrometers

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H-type x-ray spectrometer = horisontal spectrometer?

I have worked with CAMECA SX50 with the horizontal spectrometer.
It was helpful when working with fractures, for example for identification of
nonmetallic inclusions. Another it's advantage was that it was the only
spectrometer with all 4 crystals, so it had more flexibility in maps or line
scans acquisition. In quantitative analysis I did not use it both major and
trace elements acquisition and never got any problems with its performance.

Vladimir Dusevich

-----Original Message-----
} From: Laura Hernandez
To: Microscopy-at-sparc5.microscopy.com
Sent: 4/2/01 7:41 AM


Hello everybody,
We have an JEOL JXA 8600 EPMA in our Institute, with three WDS
spectrometers. We are planning to buy an other one, and we where
thinking
about the H-type x-ray spectrometer.

Is there anybody in the list who has experience with this kind of
spectrometers that could give me some information about them (their
performance in general and also comparatively to standard spectrometers,
etc.)?

Thanks


Laura Hernandez
Laura Hernandez
Laboratorio Microsonda Electronica
Instituto GEA
Universidad de Concepcion
Casilla 160C
Concepcion
CHILE

e-mail: lahernan-at-udec.cl
FAX: 56-41-242535
TELEFONO:56-41-204861




From daemon Mon Apr 2 17:50:34 2001



From: SEM Machine :      SEM-at-ACATC.AME.Arizona.edu
Date: Mon, 2 Apr 2001 15:48:48 -0700
Subject: Creating reference points on SEM sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to determine a method to create or embed points of reference on
an SEM sample. To give some background, the samples are sections of
microprocessor packages that are placed in a modified stage with a
three-point bend fixture. What I'm trying to do is monitor the movement of
points on the specimen surface as the load is increased. I tried using the
spot mode on the scope to see if I could remove some of the sputter coat,
since most of the material underneath is non-conductive, but that was
unsuccessful.

Imaging will most likely be done between 300 and 1000x. I need to have
multiple reference points on the screen at one time, since I will be
measuring relative displacements. The reference points do not need to be
distributed in a uniform pattern.

I have tried applying some powder to the surface, since I read about this
being done before, but the results were not acceptable. I may just be using
the wrong type of powder, but I haven't been able to find out what kind of
powders would work best

So, any ideas on how I may accomplish this?


Thanks,

Norman Kay
Graduate Student
AME Dept.
The University of Arizona




From daemon Mon Apr 2 18:08:49 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 3 Apr 2001 11:11:15 GMT+1200
Subject: Camera-Microscope interfacing

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I want to put a (cheap) video camera onto the optical microscope of
my JEOL 840.

I want to leave the eyepiece lens on, so that users can have the
option of easily removing the camera to use the microscope
conventionally.

I have tried presenting several different models of CCD video cameras
up to the eyepiece, both with and without the camera lens attached,
but none gives me anything better than a smallish bright circle in
the centre of the (black) field of view.

I presume that I need some sort of intermediate lens, but my
understanding of physical optics has largely evaporated over the
years.

Can someone point me towards a suitable text or other information
source?

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Apr 2 23:23:34 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 2 Apr 2001 23:17:00 -0500
Subject: Re: Camera-Microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
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} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
} I want to put a (cheap) video camera onto the optical microscope of
} my JEOL 840.
}
} I want to leave the eyepiece lens on, so that users can have the
} option of easily removing the camera to use the microscope
} conventionally.
}
} I have tried presenting several different models of CCD video cameras
} up to the eyepiece, both with and without the camera lens attached,
} but none gives me anything better than a smallish bright circle in
} the centre of the (black) field of view.
}
} I presume that I need some sort of intermediate lens, but my
} understanding of physical optics has largely evaporated over the
} years.
}
} Can someone point me towards a suitable text or other information
} source?
}
} thanks
}
} rtch

A CCD camera without a lens looking into and eyepiece usually has the
opposite problem of having too much magnification. The image coverage of
the image on the CCD camera can be increased when no lens is present on
the camera simply by moving the camera further away from the eyepiece. My
set up uses a 2.6x eyepiece for the video camera and a 10 x eyepiece to
view the distance between the 2.6 eyepiece and the CCD element is about 2
inches or a little more and I have almost twice the magnification on the
CCD camera as I see through the 10X eyepiece.

So just adding a spacer between your camera and your eyepiece should solve
your problem.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger





From daemon Tue Apr 3 01:22:48 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 3 Apr 2001 07:21:39 +0100 (GMT Daylight Time)
Subject: Re: Creating reference points on SEM sample

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Hi Norman,

If you have coat your specimen with a thin carbon layer for
conduction then add another layer of gold through a TEM
grid as a mask you should be able to see the grid bars.
Check out your local EM supplier's catalogue for the most
suitable grid design.

Good luck,
Ron

On Mon, 2 Apr 2001 15:48:48 -0700 SEM Machine
{SEM-at-ACATC.AME.Arizona.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am trying to determine a method to create or embed points of reference on
} an SEM sample. To give some background, the samples are sections of
} microprocessor packages that are placed in a modified stage with a
} three-point bend fixture. What I'm trying to do is monitor the movement of
} points on the specimen surface as the load is increased. I tried using the
} spot mode on the scope to see if I could remove some of the sputter coat,
} since most of the material underneath is non-conductive, but that was
} unsuccessful.
}
} Imaging will most likely be done between 300 and 1000x. I need to have
} multiple reference points on the screen at one time, since I will be
} measuring relative displacements. The reference points do not need to be
} distributed in a uniform pattern.
}
} I have tried applying some powder to the surface, since I read about this
} being done before, but the results were not acceptable. I may just be using
} the wrong type of powder, but I haven't been able to find out what kind of
} powders would work best
}
} So, any ideas on how I may accomplish this?
}
}
} Thanks,
}
} Norman Kay
} Graduate Student
} AME Dept.
} The University of Arizona
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Apr 3 02:00:43 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Tue, 3 Apr 2001 01:57:33 -0500
Subject: RE: Creating reference points on SEM sample

Contents Retrieved from Microscopy Listserver Archives
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The magnifications you are using present the problem. I'm sure that you
are also wanting as much resolution as possible from image processing of
the resultant images.

Producing a non-conductive spot on the sample is a good idea, as it should
stand out well, particularly during a slow record mode scan.

My vote - copier or laser printer toner. Very small particle size,
non-conductive plastic composition. Also, once the powder is sprinkled on,
it can be adhered to the surface with a little heat to ensure that it
doesn't move around.

On Monday, April 02, 2001 5:49 PM, SEM Machine
[SMTP:SEM-at-ACATC.AME.Arizona.edu] wrote:
}
}
} I am trying to determine a method to create or embed points of reference
on
} an SEM sample. To give some background, the samples are sections of
} microprocessor packages that are placed in a modified stage with a
} three-point bend fixture. What I'm trying to do is monitor the movement
of
} points on the specimen surface as the load is increased. I tried using
the
} spot mode on the scope to see if I could remove some of the sputter coat,
} since most of the material underneath is non-conductive, but that was
} unsuccessful.
}
} Imaging will most likely be done between 300 and 1000x. I need to have
} multiple reference points on the screen at one time, since I will be
} measuring relative displacements. The reference points do not need to be
} distributed in a uniform pattern.
}
} I have tried applying some powder to the surface, since I read about this
} being done before, but the results were not acceptable. I may just be
using
} the wrong type of powder, but I haven't been able to find out what kind
of
} powders would work best
}
} So, any ideas on how I may accomplish this?
}
}
} Thanks,
}
} Norman Kay
} Graduate Student
} AME Dept.
} The University of Arizona
}
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Tue Apr 3 02:30:27 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 3 Apr 2001 09:25:05 +0200
Subject: Lorentz microsopy

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My collegue C. Ulhaq want to know who practice Lorentz Microscopy on TEM
(in Europe particulary).

The questions would be : which kind of microscope you use, do you use
special polar pieces, and did you buy it or were they "home made". Same
question about the sample holder. What are the max magnification
accessible ? We have a ABT Topcon 002B, with the the possibility to change
the polar pieces.

You can answer direct to my collegue (corinne.ulhaq-at-ipcms.u-strasbg.fr),
or on the list.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Apr 3 02:35:06 2001



From: electron microscope laboratory :      emlab-at-udsm.ac.tz
Date: Tue, 03 Apr 2001 10:30:57 +0300
Subject: SCHOLARSHIP QUERRY, RE-STATED

Contents Retrieved from Microscopy Listserver Archives
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Hi All!

Thanks for the replies and the advice that you have offered.

I have Some additional information.
I am a BSc.[Physics, Mathematics] holder. Currently I am working at the E.M
laboratory of the university of
Dar es Salaam as a supporting staff, and I am looking for the opportunity
to pursue an MSc. and hence a Ph.D
that will qualify me to research fellow. Funds are a hindrance. We have one
old ZEISS9S2 TEM, and a new
LEO 910 analytical TEM.
An MSc. course in TEM or a research in metals/ceramics will do.

Thanks again,
Maulid
..........................................

Maulid Kivambe
University of Dar es Salaam
Faculty of Science
P.O.Box 35065
Dar es Salaam
Tanzania.

Phone +255 0744 266667
Fax +255 0222 410258
E-mail Mkivambe-at-hotmail.com
.........................................


From daemon Tue Apr 3 07:58:09 2001



From: Avi David :      avi.david-at-sagitta.co.il
Date: Tue, 3 Apr 2001 07:50:00 -0500
Subject: DIC

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I need some articles about
Differential Interference Contrast (DIC)
Especially advantages and disadvantages.
Regards ,

Avi David
Application Engineer
Sagitta ES Ltd
4 Hayetzira st. Ramat-Gan 52521
Tel: + 972-3-7514601
Cell: + 972-55-765522




From daemon Tue Apr 3 09:28:18 2001



From: Karen Kelley :      klk-at-biotech.ufl.edu
Date: Tue, 03 Apr 2001 10:19:38 -0500
Subject: culture in paraffin

Contents Retrieved from Microscopy Listserver Archives
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Hello,
We have a client who needs to embed a cell culture in paraffin. In our lab
we embed cultures in agarose and then embed for TEM. We do not have
experience embedding paraffin. My question is, since xylene is used for
paraffin how do you keep the culture from dispersing. Any suggestions?
Thank you

Karen Kelley
Senior Electron Microscopist
University of Florida
ICBR Electron Microscopy Core Lab
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
email: klk-at-biotech.ufl.edu
http://www.biotech.ufl.edu/~emcl/staff/karenpage.html


From daemon Tue Apr 3 09:37:12 2001



From: Asli Oztan :      asost2+-at-pitt.edu
Date: Tue, 03 Apr 2001 10:42:14 -0400 (EDT)
Subject: suspension cells

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Jacques:

I have not done Lorentz microscopy (here LEM) in a long time and I am not
very familiar with the configuration of the Topcon 002B. This is what I
remember ,for LEM to work the sample has to be outside the strong magnetic
field of the OL pole piece. In the good old days, we used a modified top
entry-type specimen holder which was longer than usual so that the specimen
would sit below its normal position. In addition, the IL electronics had to
be modified so that focus could still be attained when the specimen was in
this lower position. We normally used magnifications of up to about 20 KX if
I recall.

The second way of doing this is to essentially turn off the objective lens ,
but you have to be able to focus with the IL . Some of the newer scopes
might not be able to do this without modifications to the electronics. In
our case all the modifications were done by the manufacturer.

Hope this helps

Jordi Marti

-----Original Message-----
} From: Faerber Jacques [mailto:Jacques.Faerber-at-ipcms.u-strasbg.fr]
Sent: Tuesday, April 03, 2001 3:25 AM
To: Microscopy Society of America



Hi everyone,
We are working with suspension cells (Jurkats or H9s) and we need a
protocol to prepare them for fluorescent microscope (both fixed and live
cell imaging).
Thanks in advance for your help.

Asli Oztan

asost2-at-pitt.edu
University of Pittsburgh
Molecular Virology and Microbiology



From daemon Tue Apr 3 10:47:57 2001



From: Frank Lee :      flee-at-uhnres.utoronto.ca
Date: Tue, 03 Apr 2001 11:39:46 -0400
Subject: Re: culture in paraffin

Contents Retrieved from Microscopy Listserver Archives
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You can try spinning (centrifuging) the cells down so they become a packed
ball of cells, then carefully put them into a bag (i think they are nylon
bags) for processing and subsequently into paraffin block.

Frank Lee


Karen Kelley wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
} We have a client who needs to embed a cell culture in paraffin. In our lab
} we embed cultures in agarose and then embed for TEM. We do not have
} experience embedding paraffin. My question is, since xylene is used for
} paraffin how do you keep the culture from dispersing. Any suggestions?
} Thank you
}
} Karen Kelley
} Senior Electron Microscopist
} University of Florida
} ICBR Electron Microscopy Core Lab
} Box 118525 Gainesville Florida
} Lab: 352-392-1184 fax: 352-846-0251
} email: klk-at-biotech.ufl.edu
} http://www.biotech.ufl.edu/~emcl/staff/karenpage.html



From daemon Tue Apr 3 10:56:34 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 03 Apr 2001 08:52:17 -0700
Subject: Re: Creating reference points on SEM sample

Contents Retrieved from Microscopy Listserver Archives
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Dear Norman,
When we had a similar problem of creating a reference for studying crack
growth, we used the gold-evaporated grid method, as Ron Doole mentioned.
However, this gave problems because the grid is uniform, so after you've
traveled a little way, you had no unique reference to keep you placed. We
solved this by also puting a drop of latex sphere suspension on the surface,
before sputter coating. This is a suspension of latex spheres of specific
size, I believe we used one micron, but you can use a size suitable to your
magnification. The suspension dries to form a random pattern of dots that
can be compared in photos. You amy have to experiment with the concentration
of spheres to get the right coverage. We were lucky enough to have a
researcher making latex spheres who gave us some of her duds, but these
suspensions can be purchased.
I hope this helps.
At 03:48 PM 4/2/01 -0700, you wrote:
}
} I am trying to determine a method to create or embed points of reference on
} an SEM sample. To give some background, the samples are sections of
} microprocessor packages that are placed in a modified stage with a
} three-point bend fixture. What I'm trying to do is monitor the movement of
} points on the specimen surface as the load is increased. I tried using the
} spot mode on the scope to see if I could remove some of the sputter coat,
} since most of the material underneath is non-conductive, but that was
} unsuccessful.
}
} Imaging will most likely be done between 300 and 1000x. I need to have
} multiple reference points on the screen at one time, since I will be
} measuring relative displacements. The reference points do not need to be
} distributed in a uniform pattern.
}
} I have tried applying some powder to the surface, since I read about this
} being done before, but the results were not acceptable. I may just be using
} the wrong type of powder, but I haven't been able to find out what kind of
} powders would work best
}
} So, any ideas on how I may accomplish this?
}
}
} Thanks,
}
} Norman Kay
} Graduate Student
} AME Dept.
} The University of Arizona
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Apr 3 13:24:27 2001



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Tue, 3 Apr 2001 13:46:51 -0400
Subject: methylamine tungstate

Contents Retrieved from Microscopy Listserver Archives
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Can someone identify a supplier for small amounts of methlyamine tungstate
powder (used for negative staining in TEM)? I have tried EMS, Ted Pella,
and Polysciences (the supplier of our original decade-old vial).
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396


From daemon Tue Apr 3 13:29:01 2001



From: Ellen Carrillo-Heian :      emheian-at-engr.ucdavis.edu
Date: Tue, 3 Apr 2001 11:25:45 -0700 (PDT)
Subject: ESCA/Auger value

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I'm trying to get a handle on the value of an old Auger/ESCA spectrometer.
The model is PHI 558, by Perkin-Elmer. It was built around 1985 or 86, and
the electronics are quite old, but it's intact and fully functional. It
also has a LEED detector and has been upgraded over time. A partial list
of parts is included below. Does anyone have an idea what this might be
worth?

Thank you,
Ellen Carrillo-Heian

emheian-at-engr.ucdavis.edu
Dept. of Chem. Eng. and Mat. Sci.
UC Davis
Davis, CA 95616
USA
----------------------
Partial list of components:

} 32-010 Lock In Amp
} 20-0275 Electron Multiplier Supply
} 32-095 X Ray Source Control
} 22-040 DC Power Supply
} 16-020 Heat Exchange / Deionizer
} 20-805 Analyzer Control
} 32-100 Electron Multiplier Supply
} 11-065 Ion Gun Control
} 20-115 Ion Gun Control
} 11-010 Electron Gun Control
} 11-055 ESCA / Auger System Control
} 11-500A Auger System Control
} Inficon 012-214
} 04-303 Differential Ion Gun
} 04-548 Dual Anode X-ray Source
} 15-255 GAR Precision Electron Energy Analyzer
} Ultek DI Pump
} 218075B-26 UHV Instruments (Tag in front of the screw/bellows assembly, on
} frame.)
}
} And a 386 Compaq controlling it.
}
} The chamber has the dual chamber translation set up. (With the 4 ft
} screw/bellows)
}




From daemon Tue Apr 3 14:06:32 2001



From: Paul.Nolan-at-Alcan.Com
Date: Tue, 3 Apr 2001 14:05:39 -0500
Subject: RE: Creating reference points on SEM sample

Contents Retrieved from Microscopy Listserver Archives
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We do this using contamination spots from the microscope itself.
We put down a grid array of spots using our Isis system to control the stage
and spot positons. Focus the beam to a spot and let is sit there for a minute
or so and a contamination spot should appear. We are using electropolished
aluminum. I dont know if it will work for your material but its worth a try
Then we strain our material and look at it again.
Works well.




From daemon Tue Apr 3 17:45:41 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Tue, 3 Apr 2001 15:38:51 -0700
Subject: TEM: Particle Sizing

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I would like to do high resolution TEM on spherical, semiconductor
nanoparticles. The diameters of these particles range from 1 to 10 nm. Our
goal is to acquire good digital images of these particles so that we can
size them in house. Please let me know if you can help and how much you
charge per sample or per hour. Your help will be greatly appreciated.

Regards,
Thearith



From daemon Tue Apr 3 18:06:48 2001



From: hainfeld-at-bnl.gov
Date: Tue, 03 Apr 2001 18:06:51 -0500
Subject: methylamine tungstate

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Palmer,
Nanoprobes sells the methylamine tungstate you desired under the name
"NanoW". It is an excellent negative stain. They also make methylamine
vanadate, a similar, but lower atomic number stain they call "NanoVan".
More information is at www.nanoprobes.com.
J. Hainfeld


Dr. James F. Hainfeld
Brookhaven National Laboratory
Biology Dept.
Bldg. 463
Upton, NY 11973 USA
Tel. 631-344-3372
Fax. 631-344-3407
email: hainfeld-at-bnl.gov
website: http://bnlstb.bio.bnl.gov/biodocs/stem/stem.html


From daemon Tue Apr 3 19:29:10 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Tue, 3 Apr 2001 17:50:22 -0700
Subject: Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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Generally, equipment depreciates at the rate of 30% per year on the balance.
In other words, an SEM with an initial cost of 100K is worth 70K after the
first year, 49K the second year, 34.3k the third year, etc.

These numbers are based upon the experience I have had with used equipment
sales during the past five years.
Other allowances are made for equipment that has been abused, etc.
Strangely enough, accessories add little to the resale value of equipment.

I am sure there are others who would differ as it would not fit into their
accounting procedures but my experience has been that scientific equipment
depreciates more than computers.

Regards,

Earl Weltmer

I have no financial interest in this thread only experience.

----- Original Message -----
} From: "Ellen Carrillo-Heian" {emheian-at-engr.ucdavis.edu}
To: "microscopy" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, April 03, 2001 11:25 AM



Hi everybody (again),

I am looking for a program that allows me to quantify a given group of
nanoparticles that have various shapes and sizes. These particles range from
1 to 10 nm in diameter. Your help will be greatly appreciated. Thank you.

Regards,
Thearith





From daemon Tue Apr 3 21:37:30 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Tue, 03 Apr 2001 22:36:56 -0400
Subject: Re: methylamine tungstate - commercial vendor reply

Contents Retrieved from Microscopy Listserver Archives
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Hello Robert:

We sell this as a 2 % aqueous solution (suitable for use directly) - the
product is called "Nano-W." It's listed on our web site catalog under
"Negative staining."

Regards,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (919)
845-6324

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Tue Apr 3 23:04:23 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 Apr 2001 21:04:57 -0700
Subject: Re: ESCA/Auger value

Contents Retrieved from Microscopy Listserver Archives
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Earl:

What kind of computer depreciates (looses value) slower than
scientific equipment? A regular PC or Mac drops by over
50% the first year. After an additional six months, the system
worth next to nothing. But of course, the new and improved
model is $2K or more. Either way, the standard IRS depreciation
schedule for computers and scientific equipment is five years.
I would say that this is more appropriate for scientific equipment
than it is for computers. But YMMV.

gary g.


At 05:16 PM 4/3/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Apr 4 00:58:12 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Wed, 4 Apr 2001 15:47:39 +1000
Subject: equipment depreciation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is an interesting thread. Our unit is supposed to operate at "full
cost recovery", and as such we are charged depreciation of the instruments
(funded from a central equipment grant), which are depreciated over 15
years. Only computers are depreciated over 3 years. If anyone else is
aware of some vaguely standard depreciation times for TEMs, SEMs, confocal,
light microscopes, I'd appreciate hearing about this.

Thanks,
rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Wed Apr 4 02:40:34 2001



From: loidrgiperikg-at-netian.com
Date: Tue, 03 Apr 2001 23:35:54 -1900
Subject: .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Wed Apr 4 07:19:55 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 4 Apr 2001 09:01:23 -0300
Subject: Re: equipment depreciation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rosemary said -
} This is an interesting thread. Our unit is supposed to operate at "full
} cost recovery", and as such we are charged depreciation of the instruments
} (funded from a central equipment grant), which are depreciated over 15
} years.

FWIW, I understand that that our organization (A Canadian federal government
one) also depreciates this kind of major capital equipment over 15 years.
(Except, apparently, helicopters - our Navy is still using ones which are,
literally, older than most of the pilots flying them....)

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia




From daemon Wed Apr 4 07:24:34 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 04 Apr 2001 08:20:55 -0400
Subject: RE: adjust the intensity of the image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Russ recently demonstrated an adaptive equalization technique for us
that works quite well. Basically it is histogram equalization over a local
area of the image that is then stepped over the whole image. He has
implemented this method in his IP Toolkit and Fovea Pro packages that
plugin to PhotoShop. It is also described in The Image Processing
Handbook, 2nd edition on page 222.

Just a satisfied customer...

Henk Colijn

At 11:42 AM 4/2/01 -0400, Walck, Scott D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Wed Apr 4 07:29:14 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 4 Apr 2001 05:25:31 -0700 (PDT)
Subject: Re: equipment depreciation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An interesting thread indeed! We are in the midst of surplusing out a lot
of used histology equipment, and find that there are two different methods
of determining value. Depreciation of equipment for tax purposes is done
according to state/federal tax laws--and I think that means after 5 or 10
years (the time seems to change, and I can never keep up with) the _tax_
value is zero. However. When it comes to disposing of the equipment, our
business folks are using a different "book value" that has values
significantly greater than current market value. So.... Draw your own
conclusions.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Wed, 4 Apr 2001 15:47:39 +1000, Rosemary White wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| This is an interesting thread. Our unit is supposed to operate at "full
| cost recovery", and as such we are charged depreciation of the
instruments
| (funded from a central equipment grant), which are depreciated over 15
| years. Only computers are depreciated over 3 years. If anyone else is
| aware of some vaguely standard depreciation times for TEMs, SEMs,
confocal,
| light microscopes, I'd appreciate hearing about this.
|
| Thanks,
| rosemary
|
|
| Rosemary White
| Microscopy Centre
| CSIRO Plant Industry
| GPO Box 1600
| Canberra, ACT 2601
| Australia
|
| phone 61-2-6246 5475
| fax 61-2-6246 5000
| email r.white-at-pi.csiro.au
|
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed Apr 4 08:18:02 2001



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Wed, 4 Apr 2001 09:12:26 -0400
Subject: RE: equipment depreciation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that CSIRO is on the high side. In the Canadian government 10 years
seems to have been the semi-official standard for as long as I can remember.
In my lab, though, we informally think of two 'effective lifetimes', 7 years
or thereabouts for the TEM and SEM, and 10 for the other beam instruments
(EPMA, SIMS, XPS and SAM). Furthermore, we regard these as upper limits for
two reasons:

- vendors these days are operating on ever-tighter parts inventories, thus a
beam instrument may have some good years left in it, but you find you can't
get a crucial part any longer. This happened to us recently with our 12
year old Cameca SIMS, when a flight tube developed ultrafine cracks and we
discovered that they are not kept in stock any more. After a lot of
arm-twisting and calling of favors, we tracked down one of the few remaining
ones in Europe, otherwise we would have been down for 3 months awaiting a
custom-built one.

- we do a lot of work with the private sector, some on a contract basis.
They often come to us to get state-of-the-art data quality which has been
collected in a timely fashion. The first is what usually gets their
attention in the first place, while the second is crucial to keeping their
attention.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


} ----------
} From: Rosemary White
} Sent: Wednesday, April 04, 2001 1:47 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: equipment depreciation
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is an interesting thread. Our unit is supposed to operate at "full
} cost recovery", and as such we are charged depreciation of the instruments
} (funded from a central equipment grant), which are depreciated over 15
} years. Only computers are depreciated over 3 years. If anyone else is
} aware of some vaguely standard depreciation times for TEMs, SEMs,
} confocal,
} light microscopes, I'd appreciate hearing about this.
}
} Thanks,
} rosemary
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} phone 61-2-6246 5475
} fax 61-2-6246 5000
} email r.white-at-pi.csiro.au
}
}
}


From daemon Wed Apr 4 08:18:02 2001



From: mxm67-at-email.psu.edu
Date: Wed, 4 Apr 2001 09:09:04 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe....


From daemon Wed Apr 4 09:40:19 2001



From: Boucher, Germaine G :      germaine_g_boucher-at-groton.Pfizer.com
Date: Wed, 4 Apr 2001 10:34:52 -0400
Subject: TEM: precipitate in samples of white fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am processing samples of white fat for TEM and have been having trouble
with a dense precipitate that covers the cytoplasm. Nuclei and blood
vessels are relatively unaffected. So far I've tried 3 different fixatives:
Trump's and 2.5% glut/2% para in either 0.1M cacodylate of 0.1M phosphate
buffer. The samples have been osmicated for one hour and embedded in either
Spurr's resin or Epon. The precipitate is present in unstained sections as
well as those stained with UA alone or UA and lead. Treatment with .5N HCl
for 2 minutes alleviates the problem somewhat but bleaches the sections so
much that they are very difficult to see. If anyone has encountered similar
problems and has suggestions for me I would be most grateful.

Thanks in advance

Germaine G. Boucher
TEM Lab
Pfizer Global Research and Development
Groton, CT




From daemon Wed Apr 4 10:18:06 2001



From: jeanross :      jeanross-at-blue.weeg.uiowa.edu
Date: Wed, 4 Apr 2001 10:13:49 -0500
Subject: EDS preferences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to replace a very old EDS system on our Hitachi S-2460N
variable pressure SEM. I would like to know about your preferences for and
experiences with different companies, both good and bad. Please reply
directly to me. If anyone else is interested, I could submit a summary to the
list after I've collected all the responses.

Thanks in advance.

Jean Ross
Central Microscopy Research Facility
University of Iowa




From daemon Wed Apr 4 10:25:42 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 4 Apr 2001 10:19:39 -0500
Subject: Confocal Service contracts

Contents Retrieved from Microscopy Listserver Archives
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I have two questions concerning service contracts on confocals. Let
me start by saying i have had a confocal for about 8 years and would
never consider going without one. I have a Biorad 2000 going off
warranty and need to make a decision.

First question: Biorad no longer guarantees a response time - they
now promise to get to you as fast as they can but no longer promise a
48 or 72 hr response. Have other confocal manufacturers done this
also?

Second question: My university is pushing replacing service
contracts with "insurance" contracts with a major vendor who then
pays for a service visit from the manufacturer on an hourly basis.
All parts, travel, service repair time, etc are covered at a price
that is typically 75% less than the manufacturer's service contract.
They guarantee the price and coverage for 3 years. Personally I
don't know how they could make money on this deal since we average a
fair number of visits and spare parts (e.g. lasers) in a typical
year. Does anyone have experience with this type of situation with
confocals? The company the University is dealing with is CIC but
there are several other ones out there.

Thanks for any input.
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Apr 4 11:03:29 2001



From: RonMervis-at-aol.com
Date: Wed, 4 Apr 2001 11:57:57 EDT
Subject: Mountant media

Contents Retrieved from Microscopy Listserver Archives
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--part1_ce.12ee3a2b.27fc9e85_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

dear listservers....
i need some advice....
we use Permount as our mountant...however, as we cut our slides extremely
thick (120 micra) for our staining method (Golgi-impregnations of neurons),
it often takes 2-3 weeks for the Permount to dry sufficiently so that we can
actually use the slides without it getting on our microscope stage, or the
coverslip moving around under our oil-immersion lenses....
so....my question is --- does any microscope maven out there know if there is
anything that can be done to accelerate the drying/hardening the
Permount....???
many thanks for any help....
regards,
Ron Mervis
~~~~~~~~~~
Ronald F. Mervis, Ph.D.
Neuro-Cognitive Research Laboratories
2109 West Fifth Avenue
Columbus, Ohio 43212 USA
~~~~~~~~~~~~~~~~~~~~~~~~~~~~
..independent nonprofit contract laboratories dedicated to quantitiative
neurostructural analysis to promote our knowledge and understanding of human
neurological diseases, neurodegeneration, and neuroplasticity....
~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Tel: (614)-486-6404; lab: (614)-486-6080
Fax: (614)-486-6020
e-mail: RonMervis-at-aol.com (or) RonMervis-at-Neuro-Cognitive.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
"...can the human soul be glimpsed through a microscope? Maybe, but you'd
definitely need one of those very good ones with two eyepieces."
- Woody Allen


--part1_ce.12ee3a2b.27fc9e85_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} dear listservers....
{BR} i need some advice....
{BR} we use Permount as our mountant...however, as we cut our slides extremely
{BR} thick (120 micra) for our staining method (Golgi-impregnations of neurons),
{BR} it often takes 2-3 weeks for the Permount to dry sufficiently so that we can
{BR} actually use the slides without it getting on our microscope stage, or the
{BR} coverslip moving around under our oil-immersion lenses....
{BR} so....my question is --- does any microscope maven out there know if there is
{BR} anything that can be done to accelerate the drying/hardening the
{BR} Permount....???
{BR} many thanks for any help....
{BR} regards,
{BR} Ron Mervis
{BR} ~~~~~~~~~~
{BR} Ronald F. Mervis, Ph.D.
{BR} Neuro-Cognitive Research Laboratories
{BR} 2109 West Fifth Avenue
{BR} Columbus, Ohio 43212 USA
{BR} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~
{BR} ...independent nonprofit contract laboratories dedicated to quantitiative
{BR} neurostructural analysis to promote our knowledge and understanding of human
{BR} neurological diseases, neurodegeneration, and neuroplasticity....
{BR} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~
{BR} Tel: (614)-486-6404; lab: (614)-486-6080
{BR} Fax: (614)-486-6020
{BR} e-mail:   RonMervis-at-aol.com (or) RonMervis-at-Neuro-Cognitive.org
{BR} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
{BR} "...can the human soul be glimpsed through a microscope?  Maybe, but you'd
{BR} definitely need one of those very good ones with two eyepieces."
{BR}                                                           - Woody Allen
{BR} {/FONT} {/HTML}

--part1_ce.12ee3a2b.27fc9e85_boundary--


From daemon Wed Apr 4 13:54:02 2001



From: Rodney McCabe :      rmccabe-at-lanl.gov
Date: Wed, 04 Apr 2001 12:48:46 -0600
Subject: SiO2 removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen some beautiful SEM pictures of semiconductor interconnect lines
(Copper and aluminum) in which the dielectric material (Silicon dioxide I
assume) has been completely removed. Can someone tell me what was used to
remove the dielectric without affecting the metallization?

Thanks

Rod



From daemon Wed Apr 4 13:54:04 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Wed, 04 Apr 2001 13:48:14 -0500
Subject: Au & Bronz

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello friends,
I have a gold bronze composition material coming in for imaging by
SEM, x-ray mapping, and optical microscopy. I could us a sample prep
recommendation, particularly for a etchant or so I think. This is
primarily a failure analysis project in which we want to clearly observe
and differentiate grain boundaries. Recommendations would be greatly
appreciated.

thanks,
Bruce Brinson
Optical Analyst
Rice University



From daemon Wed Apr 4 14:56:20 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 04 Apr 2001 15:51:36 -0400
Subject: Re: TEM: precipitate in samples of white fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Germaine,
If you don't rinse in buffer well enough after the initial fixation , the
glut will form a precipitate with osmium. Try washing 4x or 5x for 15
minutes each between glutaraldehyde and osmium.
Good luck,

At 10:34 AM 4/4/01 -0400, Boucher, Germaine G wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-5700


From daemon Wed Apr 4 16:39:05 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 5 Apr 2001 09:40:13 GMT+1200
Subject: Camera-Microscope interfacing Pt 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again

Thanks for all the recommendations for suppliers of cameras and
interfaces, but what I was wanting was a pointer to a text or
somesuch from which I could figure out myself what I need.

There must be someone in this learned and experienced community who's
been there and done that, isn't there?

"That", for those who may have missed my first post, being the
problem of how to work out what sort of intermediate lens would be
needed to interface a small cheap CCD video camera (or a webcam) so
that it gives a good image when looking into the existing eyepiece
lens of a given optical microscope (in this case the OM of a JEOL 840
SEM).

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Apr 4 18:17:28 2001



From: Kalvoda Jiri :      dino-at-sci.muni.cz
Date: Wed, 4 Apr 2001 18:15:24 -0500
Subject: digital camera for my Axiolab Zeiss microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues, I would like to buy a digital camera for my Axiolab
Zeiss microscope. Unfortunately I am a bit confused in the amount of
available data. I would need a digital camera of the resolution that
matches the quality of film cameras in order I need not scan photos or
negatives. Could you please be so kind and give me a piece of advice? I
have Olympus Camedia 3030 with 3,34 mil pixels. Will this camera and
resolution do or do I need to buy another one? If yes of what resolution
and type? I will be very indebted for an advice because I receive often
contradicting information for different distributors and I am not much
wise about it. Many thanks in advance. With best
wishes Jiri
Kalvoda Department of
Geology and Paleontology
Masaryk University
Kotlarska 2 61137 Brno
Czech republic




From daemon Wed Apr 4 18:45:15 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 04 Apr 2001 16:47:16 -0700
Subject: Re: SiO2 removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is easily done with a plasma etch using CF4 + O2.

The top layer of "glass" is typically silicon nitride over
silicon dioxide or in earlier devices, it is boron phosphor
silicon glass (BPSG).

I have some colorized shots on my web site at:

http://photoweb.net

More get added and some get swapped over time.

gary g.


At 11:48 AM 4/4/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Apr 4 19:03:46 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 04 Apr 2001 20:00:07 -0500
Subject: Removal of glass passivation layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rod McCabe wrote:
=======================================================
I have seen some beautiful SEM pictures of semiconductor interconnect lines
(Copper and aluminum) in which the dielectric material (Silicon dioxide I
assume) has been completely removed. Can someone tell me what was used to
remove the dielectric without affecting the metallization?
=======================================================
While HF and Q-Tips can be used to swab off the SiO2, the better way (in our
opinion) is with reactive plasma etching, using CF4 as the reactive gas.
This way the layer is removed in a way that does not disrurb, for example,
corrosion product that might have formed underneath. If you use the wet
chemical approach, you can dissolve and swab away features of interest, such
as corrosion product. And you have removed that which you might otherwise
have been able to analyze with EDS or even Auger.

Several manufacturers offer table top plasma etchers, SPI Supplies being one
of them. You can find information about the SPI Plasma Prep™ II on our
website given below.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Wed Apr 4 20:16:31 2001



From: James S. Martin :      james.s.martin-at-att.net
Date: Wed, 4 Apr 2001 21:08:43 -0400
Subject: clean air workstations

Contents Retrieved from Microscopy Listserver Archives
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I am writing for comments -- off-list please -- about the CleanZone LF clean
air workstation manufactured by IQAir, which, I am told, is used in
Switzerland and Germany, but only recently has been introduced in the United
States. With thanks,

James Martin
Orion Analytical, LLC
www.orionanalytical.com
martin-at-orionanalytical.com




From daemon Wed Apr 4 22:26:59 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 4 Apr 2001 17:22:23 -1000 (HST)
Subject: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

A colleague has asked for recommendations for setting up a digital
darkroom (fun to spend someone else's money!). This person would benefit
from a really good scanner that could deal with prints, large format
negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
into an Agfa Duoscan T2500. Do any of you have an opinion about this or
other suitable scanners?

I know this subject comes up regularly, but I don't feel bad about
introducing it again, since technology evolves so quickly!

Mahalo,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Apr 5 00:59:02 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Thu, 5 Apr 2001 07:51:05 +0200
Subject: tripod polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Fellow Microscopists,

thanks to everyone for the advices about all type of glues for tripod polishing.
I tried to make some sort of summary from the different answers I have
received. I would be happy if it could be any use for other tripod beginners
as well.

Cheers!
Csaba


1.) The best mail which decribes well the everyday life and joy of a
microscopist comes first.

You really can't look at a spec sheet and know that it works - it's all
trial and error.

2.) About the type of glue to be used:

The best material to use is super glue which is a cyanoacrylate material.
There are different types available under various names. They all have
slightly different properties and vary in such things as viscosity.
The most important point is that the super glue is fresh. Once it is opened,
you can use it for a short time, but then need to replace it. Sometimes,
the glue from an unopened package can also be bad if it has been on the
shelf for a long time. The trouble with using consumer products is that they
sometime change without warning.

You should buy it from a place that has a high turnover rate of it so that it is
fresh. The IBM guys recommend keeping the stuff unopened in the refrigerator
for any length of time. Once it has been opened, you can't use it for very
long (a day or two). I have used the Loctite a little longer because it has
a very good sealing top. The Loctite product is also available in a "pen" type
applicator which seems to have the best bench life of any of the applicators
I've used. My tube usually is swiped off the bench long before it goes bad.One
of the advantages to the cyanoacrylic cement is it dissolves, in a reasonable
time, in acetone.If you were to use a crosslinked epoxy, you'll need to devise
a cleaning scheme that will exhaustively remove the epoxy without altering
your sample.

Basically what we're looking for is an intermediate viscosity glue
(somewhere between maple syrup and water) that bonds in about 30-40 seconds
and is very strong. Don't use any type of super glue gel! It doesn't work,
you have to use the thin stuff.

In the end we are using a Loctite Prism 460.

Crystalbond 509. This is an acetone soluble glue which
melts at 150*C and becomes quite viscous. We use it quite often here at
Queens as a temporary glue for ceramics that have to be ground and polished
on all four sides. If the bond breaks you simply heat it up and reset the
part. This stuff comes in sticks that last for a very long time. Their
website is as follows. {http://www.aremco.com/}

I like to glue specimens with epoxy resin because the substance
does not harden too fast, and you always have time to find the best
location for your sample, so that the latter does not break.

I have tried the "super glue" approach with no luck. Many technicians
advocate Lock Tite brand of super glue that many companies sell with the
tripod polish kit. I have always utilized Crystalbond adhesive. It is a
low melting point (77 C) wax that is dissolved in acetone. If your sample
is heat sensitive, super glue is the only other choice I know.

3.)Glueing advices:

--the biggest reason for sections falling off is the cleanliness of the pedastal.
It must be cleaned with clean solvents that do not leave any trace of a
contamination film on the glass. Check for cleanliness by holding the tool
such that light reflects from the surface.

--the suface that you are glueing to should be as rough as possible to
obtain a tooth or larger surface area for the glue to adhere to.

--any way you can improve the surface area would be great.

--the same above would be for the specimen

--pressure on the sample while it cures might be important, but the IBM guys
just wick the extra stuff away from the sample and don't put a lot of
pressure on the sample.


--
____________________________________________
Csaba Cserhati
Univ.of Debrecen / Dept. of Solid State Phys.
Hungary
tel/fax: 36 52 316073
e-mail: cserhati-at-delfin.klte.hu
____________________________________________


From daemon Thu Apr 5 03:47:29 2001



From: HARRISm-at-esm-semi.co.uk
Date: Thu, 05 Apr 2001 09:36 +0000 (GMT)
Subject: SERVICE CONTRACT V INSURANCE .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I've followed with interest the discussion on equipment depreciation
and service contracts .
I've noticed over the years in both metals research and now the
semiconductor industry with increasing sophistication/specialisation
of equipment running a lab to a given budget seems to mean accepting
manufacturers service contracts with fewer 'independent' sources being
able or willing to offer maintenance assistance .

My question arises from a recent 'confocal service contract' letter
and I wonder does an insurance contract service alternative as offered
by CIC exist in the UK ? and if so could anyone let me know where I
could get more information ? .

Martyn Harris
Device Engineer
ESM Ltd , Cardiff Rd
Newport , South Wales
UK
NP10 8YJ .

Tel 01633 810121
email harrism-at-esm-semi.co.uk



From daemon Thu Apr 5 04:40:57 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 5 Apr 2001 04:38:01 -0500
Subject: RE: Confocal Service contracts

Contents Retrieved from Microscopy Listserver Archives
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First, a disclaimer. I am a third party service provider for a variety of
equipment, but not confocals. I have worked for a number of manufacturers
in the past and been self-employed for around 20 years.

In regards to the first question - service departments in general are
getting squirrellier. Contract prices have gone up greatly over the last
decade or so while quality and parts stores have gone down. A
generalization, granted, but one I'd be happy to back up.

As for the second question - don't get me started again. Given recent
problems with the industry in general, I can only suggest that your
university carefully study the question and check with several of their
customers who have been with them for at least a couple of years insuring
similar equipment. That goes for any such provider. I've been a vocal
antagonist here of the concept, and from what I have heard from some
customers, perhaps rightfully so. Not right, so far, in my feelings
regarding the potential long term problems. Rather in the broad approach
they have taken. Perhaps in trying to be a jack of all trades, they are a
master of none.

To those of you who might be surprised by my abnormally low tone in this
posting, please understand that we are getting to a point in time where
these organizations are getting quite large in a very short period of time.
As with any company or industry, they do have problems that they will not
publicize. They also have increasingly large budgets for legal teams that
would probably be anxious to root out any libel. They do save many
organizations large amounts of money, but you have to ask, at what cost?

On Wednesday, April 04, 2001 10:20 AM, Tom Phillips
[SMTP:PhillipsT-at-missouri.edu] wrote:
}
}
} I have two questions concerning service contracts on confocals. Let
} me start by saying i have had a confocal for about 8 years and would
} never consider going without one. I have a Biorad 2000 going off
} warranty and need to make a decision.
}
} First question: Biorad no longer guarantees a response time - they
} now promise to get to you as fast as they can but no longer promise a
} 48 or 72 hr response. Have other confocal manufacturers done this
} also?
}
} Second question: My university is pushing replacing service
} contracts with "insurance" contracts with a major vendor who then
} pays for a service visit from the manufacturer on an hourly basis.
} All parts, travel, service repair time, etc are covered at a price
} that is typically 75% less than the manufacturer's service contract.
} They guarantee the price and coverage for 3 years. Personally I
} don't know how they could make money on this deal since we average a
} fair number of visits and spare parts (e.g. lasers) in a typical
} year. Does anyone have experience with this type of situation with
} confocals? The company the University is dealing with is CIC but
} there are several other ones out there.
}
} Thanks for any input.
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Apr 5 07:20:17 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Thu, 5 Apr 2001 08:22:53 -0400
Subject: Re: Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thearith,

I don't know if this is exactly what you are looking for, but Reetz and Coworkers have recently
published a high troughput routine for nanaoparticle analysis: Reetz, Manfred T.; Maase, Matthias;
Schilling, Tobias; Tesche, Bernd. Computer Image Processing of Transmission Electron Micrograph
Pictures as a Fast and Reliable Tool To Analyze the Size of Nanoparticles. J. Phys. Chem. B
(2000), 104(37), 8779-8781.

Good Luck,

Andreas

*************************************************
Dr. Andreas Taubert
Dept. of Materials Science and Engineering
3231 Walnut Street
University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************




From daemon Thu Apr 5 07:28:54 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 5 Apr 2001 05:25:43 -0700 (PDT)
Subject: Re: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina:
We have the Duoscan 1200 but the 2500 is also a very nice unit--additional
(real) resolution and a high O.D. range plus 14 or 16 bit image depth. The
Agfa units also come with a built-in transparency plate (rather than having
to add on a separate transparency adaptor). I find that color fidelity is
very good with the Duoscan, and Agfa provides both reflective and
transparency calibration standards. I am currently scanning in 3x4 TEM
negatives at 12 bits (yield is about 26MB per image), and scan time is
fairly rapid. There is one option that you might want to consider: the
DIImage unit is made for up to 4x5 negatives, and I think (can't remember
the last time I read the specs--the neurons aren't firing today) that
resolution is in the 2700 dpi range--even for the 4x5 size.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

The opinions expressed are solely my own and do not constitute an
endorsement of any vendor or manufacturer. I have no fiduciary interest in
either company.

On Wed, 4 Apr 2001 17:22:23 -1000 (HST), Tina Carvalho wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hi, All-
|
| A colleague has asked for recommendations for setting up a digital
| darkroom (fun to spend someone else's money!). This person would benefit
| from a really good scanner that could deal with prints, large format
| negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
| into an Agfa Duoscan T2500. Do any of you have an opinion about this or
| other suitable scanners?
|
| I know this subject comes up regularly, but I don't feel bad about
| introducing it again, since technology evolves so quickly!
|
| Mahalo,
| Tina
|
| http://www.pbrc.hawaii.edu/bemf/microangela
|
****************************************************************************
| * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
*
| * Biological Electron Microscope Facility * (808) 956-6251
*
| * University of Hawaii at Manoa *
http://www.pbrc.hawaii.edu/bemf*
|
****************************************************************************
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Thu Apr 5 08:32:17 2001



From: rgriffin-at-eng.uab.edu
Date: Thu, 5 Apr 2001 08:24:54 -0500
Subject: Preparation of a steel wedge on a ceramic substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a professor here who has a 1 cm x 2.5cm steel layer about 100 um in
thickness on a ceramic substrate. The metal layer was sputter deposited
onto the ceramic substrate. The ceramic substrate extends past the metal
layer. He needs to get a thickness gradient across the steel layer along
the 2.5 cm length. He would like to have one end at about 50 microns in
thickness and the other at 2 microns with a gradually decreasing thickness
gradient. Steps down would be ok although a smooth transition would be
better. We have a laser profilometer to measure anything that we produce.

Does anyone out there have any ideas how to do this? Would a tripod
polisher work? I thought about electropolishing and masking off portions at
a time but I worry about what will happen at the interface between the
ceramic and the metal. Could we alter a dimpler?



Thanks,

Robin Griffin
UAB


From daemon Thu Apr 5 08:39:35 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 5 Apr 2001 08:38:46 -0500
Subject: Film Scanners - Rule of Thumb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina

There is a simple rule of thumb I use.

Nominal grain size of film is about 10 microns (varies
with film speed etc but this is the right order of magnitude).
Thus to digitize the film to it's nominal limits your scanner should
be able to digitize to better than this spatial dimension.

A simple back of the envelope calculation says a spatial resolution
of 10 microns is 2540 - dpi..... and as
we all know that must be the optical resolution of the
scanner not the interpolated resolution. Scanners at this
end are obviously more than you need to digitize photo's and
get expensive quickly. Also when you see 2 numbers listed
as the scanners resolution, believe only the first number, that is
the CCD resolution.

Now add your bit depth. 12 bits is the minimium I
would shoot for greyscale image, but if your attempting
diffraction work the higher the better (i.e. 14 -16 bits+).
For color work obviously multiple the bit depth by 3
one for each primary color (RGB). I've seen a number
of 36 bit color scanners but not too many 48 bit ones at
} 2540 dpi.

Lastly, dit depth is irrelevant if you don't have a high
optical density capabilities otherwise your just digitizing
noise. The highest value I believe is an OD of 4.0 but
this is for DRUM scanners. Flatbed scanners typically
run as low as 2.8, upwards to about 3.4 for the best
I've seen in a flatbed.

Hope that helps...


Nestor
Your Friendly Neighborhood SysOp.




From daemon Thu Apr 5 08:51:02 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 05 Apr 2001 08:46:30 -0500
Subject: Re: Camera-Microscope interfacing Pt 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 09:40 AM 4/5/01 +0000, Ritchie Sims wrote:
} "That", for those who may have missed my first post, being the
} problem of how to work out what sort of intermediate lens would be
} needed to interface a small cheap CCD video camera (or a webcam) so
} that it gives a good image when looking into the existing eyepiece
} lens of a given optical microscope (in this case the OM of a JEOL 840
} SEM).

You might try a web search for "afocal coupling".
See http://www.photosolve.com/xtendascope.asp for example.

This page, and the message below, are for coupling to
the eyepiece of telescopes, but the process should be similar
with a microscope eyepiece. If you're looking for "small and cheap",
put in a wide field eyepiece and hold the camera close to it
and see what happens. I suspect you'll have some cropping
of the image due to optical mismatch.

- John

} A friend of mind recently bought a fairly expensive SLR digital still
} camera, an Olympus C-2500L,
}
} http://www.olympusamerica.com/product.asp?c=57&p=16&s=12&product=380
}
} and has experimented with afocal photography through both his 8-inch f/6
} Dob and a microscope. All he sees in the camera is a small central disk
} of light. He got the following explanation from a post on rec.photo.
} digital.
}
} For cameras with LARGE taking lenses (f 2.8, f2.0) [...] there will
} be a problem since their entry pupil ( the area of the lens that lets
} the image into the lens) is so large compared to the exit pupil (the
} opening on the eyepiece that lets the image out of the microscope)
} that a large amount of the image will be lost!! This results in
} severe vignetting, and so only a small central spot of image in a
} dark field is recorded by the digital camera.
}
} Does this sound reasonable? The optics of this situation are pretty
} mysterious to me, so I can't judge. But it seems like increasing the
} focal length of the camera lens (zooming in) should compensate for the
} effect of the small exit pupil, and that the problem might be more one
} of eye relief (he just can't get the lens close enough to the eyepieces,
} which I think are a couple of Plossls and the 25mm SMA that comes with
} Celestron Dobs).

And here's the response so far.

} Subject: Re: afocal astrophoto problem: exit pupil?
} Date: Wed, 4 Oct 2000 17:52:19 -0400
} From: "Michael A. Covington" {See http://www.CovingtonInnovations.com for address}
} Organization: MindSpring Enterprises
} Newsgroups: sci.astro.amateur
}
} } Does this sound reasonable?
}
} No. The camera lens is *supposed* to have a larger entrance pupil than the
} exit pupil of the telescope. When doing afocal photography with an SLR the
} entrance pupil of the lens is an inch or more in diameter.
}
} If there is vignetting, it's probably because the camera is the wrong
} distance from the eyepiece -- either too close or too far.
}
} --
}
} Clear skies,
}
} Michael A. Covington / AI Center / The University of Georgia
} Author, ASTROPHOTOGRAPHY FOR THE AMATEUR
} http://www.CovingtonInnovations.com/astro {} {
}
}
} Subject: Re: afocal astrophoto problem: exit pupil?
} Date: Wed, 4 Oct 2000 14:25:48 -0700
} From: "Bob May" {bobmay-at-nethere.com}
} Organization: Posted via Supernews, http://www.supernews.com
} Newsgroups: sci.astro.amateur
}
} Bet that when you look at the viewfinder, you will see an image just
} like one that you would see if your eye were at a certain distance
} from the EP. If that image looks like the eye is too far away from
} the EP then it's a sure thing that the camera's lens is too far away
} from the EP. That's how it's all done. The camera is nothing more
} than an aritificial eye.
} --
} Bob May
} Remember that computers do exactly what you tell them to do, not what
} you think that you told them!
} Bob May
}
}
} Subject: Re: afocal astrophoto problem: exit pupil?
} Date: Wed, 04 Oct 2000 19:43:43 GMT
} From: "Chuck Olson" {chuckolson01-at-home.com}
} Organization: -at-Home Network
} Newsgroups: sci.astro.amateur
}
} Yes, it is critical that the eyepiece and camera lens be somewhat
} physically compatible with each other. The eyepiece must put the
} exit pupil about in the plane of the camera iris opening, which
} in most instances requires the eyepiece to be virtually in
} contact with the camera lens front element. For instance, the
} Nikon Coolpix 950 and 990 have relatively small lens fronts and a
} nice 28mm (I think) thread that adapts readily to the T-thread
} that is often used in astrophotography. As a result, the CP950
} easily looks through 17mm , 26mm, or 32mm Plossl eyepieces. Even
} there, as you point out, the camera needs to be operating at the
} tele end of its zoom range to fill the rectangular frame, rather
} than showing a small, circular, fuzzy-edged, wide-angle field.
}
} I'm not sure what the C-2500L looks like, but it may have a
} physically larger lens that has its iris deep behind the front
} surface. This might require a very long focus eyepiece, like a
} 40mm Plossl, conceivably, or one with even greater eye relief, to
} accomplish the optical hook-up more favorably, and may limit the
} operation of the overall system to somewhat lower magnifications.
} Oh, once you have a compatible eyepiece, then you can use Barlow
} lenses to get back needed magnification for your desired image
} scale. The only probmen there is the setup gets pretty long as
} these lenses are stacked up, and stability may suffer.
}
} The Nikon, as mentioned, has been found by many to be almost
} ideal for afocal photography of the moon and planets.
}
} Chuck
}



From daemon Thu Apr 5 09:02:28 2001



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Thu, 05 Apr 2001 09:10:42 -0500
Subject: FRET/FLIM Symposium Second Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{bigger} The University of Texas Health Science Center will host a
symposium sponsored by Hamamatsu Photonics KK

on

{/bigger}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} {bigger} FRET
and FLIM:

{/bigger} {/bigger} {/bigger} {/color} {/bold} {bold} {bigger} Advanced
Fluorescence Techniques for Biological Imaging

{/bigger} {/bold}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} June 8-10,
2001 {/bigger} {/bigger} {/color} {/bold} =20


{bold} at {/bold} =20


{bold} {bigger} The Sheraton Gunter Hotel

205 E. Houston St.

San Antonio, TX


************************************************************************* {/b=
igger} {/bold} Interest
in the sophisticated fluorescence imaging techniques of Fluorescence
Resonance Energy Transfer (FRET) and Fluorescent Lifetime Imaging
Microscopy (FLIM) amongst the biological research community has grown in
recent years. FRET imaging provides a tool to solve complex structural
associations at resolution limits beyond conventional optical imaging.
FLIM allows the measurement of FRET without the significant problems
associated with intensity based FRET measurement, as well as faster, more
accurate and quantitative measurement of cell physiology. These
techniques are also being implemented in high-throughput screening
regimes for drug discovery. Invited lectures by a distinguished group of
scientists will concentrate on new technical developments in these areas
and demonstrate successful application of these techniques in biological
and industrial settings.=20


A poster session has been organized for June 9th so that registrants may
present their experiences with FRET and FLIM. =20


We anticipate that this will be a most enjoyable as well as
intellectually stimulating symposium.=20


{bold} **********************************************************************=
***********

{bigger} Speakers:

{/bigger}

Philippe Bastiaens, European Molecular Biology Laboratory (Germany)=20

Christoph Biskup, Friedrich Schiller University (Germany)=20

Robert Clegg, University of Illinois Urbana-Champaign (USA)=20

Michael Edidin, Johns Hopkins University (USA)=20

Hans Gerritsen, Utrecht University (Netherlands)=20

Jesus Gonzalez, Aurora Biosciences (USA)=20

Enrico Gratton, University of Illinois Urbana-Champaign (USA)=20

Brian Herman, University of Texas Health Science Center San Antonio (USA)=20

Thomas Jovin, Max-Planck Institute for Biophysical Chemistry (Germany)=20

Steven Kay, The Scripps Research Institute/Novartis Inc.(USA)=20

Karsten K=F6nig, Friedrich Schiller University (Germany)=20

Wen-Hong Li, University of Texas Southwestern Medical Center (USA)=20

Atsushi Miyawaki, The Institute of Physical and Chemical Research (Japan)=20

Ammasi Periasamy, University of Virginia (USA)=20

Alexander Sorkin, University of Colorado Health Science Center (USA)=20

Roger Tsien, University of California, San Diego (USA) {/bold} =20


{bold} {color} {param} 0000,0000,ffff {/param} {bigger}

Registration Fees

Student: $175 ($200 after May 1st)

Academic/Corporate: $225 ($250 after May 1st)

{/bigger} {/color} {/bold}


Meeting, lodging and travel information may be found at:


{bold} {color} {param} ffff,0000,ffff {/param} {bigger} {bigger} http://usa.hamamat=
su.com/fretflim

{/bigger} {/bigger} {/color} {/bold}

or contact


Victoria Centonze Frohlich (mailto:frohlich-at-uthscsa.edu)


=09





From daemon Thu Apr 5 09:06:44 2001



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Thu, 05 Apr 2001 09:15:11 -0500
Subject: FRET/FLIM Symposium Second Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{bigger} The University of Texas Health Science Center will host a
symposium sponsored by Hamamatsu Photonics KK

on

{/bigger}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} {bigger} FRET
and FLIM:

{/bigger} {/bigger} {/bigger} {/color} {/bold} {bold} {bigger} Advanced
Fluorescence Techniques for Biological Imaging

{/bigger} {/bold}

{bold} {color} {param} ffff,0000,0000 {/param} {bigger} {bigger} June 8-10,
2001 {/bigger} {/bigger} {/color} {/bold} =20


{bold} at {/bold} =20


{bold} {bigger} The Sheraton Gunter Hotel

205 E. Houston St.

San Antonio, TX


************************************************************************* {/b=
igger} {/bold} Interest
in the sophisticated fluorescence imaging techniques of Fluorescence
Resonance Energy Transfer (FRET) and Fluorescent Lifetime Imaging
Microscopy (FLIM) amongst the biological research community has grown in
recent years. FRET imaging provides a tool to solve complex structural
associations at resolution limits beyond conventional optical imaging.
FLIM allows the measurement of FRET without the significant problems
associated with intensity based FRET measurement, as well as faster, more
accurate and quantitative measurement of cell physiology. These
techniques are also being implemented in high-throughput screening
regimes for drug discovery. Invited lectures by a distinguished group of
scientists will concentrate on new technical developments in these areas
and demonstrate successful application of these techniques in biological
and industrial settings.=20


A poster session has been organized for June 9th so that registrants may
present their experiences with FRET and FLIM. =20


We anticipate that this will be a most enjoyable as well as
intellectually stimulating symposium.=20


{bold} **********************************************************************=
***********

{bigger} Speakers:

{/bigger}

Philippe Bastiaens, European Molecular Biology Laboratory (Germany)=20

Christoph Biskup, Friedrich Schiller University (Germany)=20

Robert Clegg, University of Illinois Urbana-Champaign (USA)=20

Michael Edidin, Johns Hopkins University (USA)=20

Hans Gerritsen, Utrecht University (Netherlands)=20

Jesus Gonzalez, Aurora Biosciences (USA)=20

Enrico Gratton, University of Illinois Urbana-Champaign (USA)=20

Brian Herman, University of Texas Health Science Center San Antonio (USA)=20

Thomas Jovin, Max-Planck Institute for Biophysical Chemistry (Germany)=20

Steven Kay, The Scripps Research Institute/Novartis Inc.(USA)=20

Karsten K=F6nig, Friedrich Schiller University (Germany)=20

Wen-Hong Li, University of Texas Southwestern Medical Center (USA)=20

Atsushi Miyawaki, The Institute of Physical and Chemical Research (Japan)=20

Ammasi Periasamy, University of Virginia (USA)=20

Alexander Sorkin, University of Colorado Health Science Center (USA)=20

Roger Tsien, University of California, San Diego (USA) {/bold} =20


{bold} {color} {param} 0000,0000,ffff {/param} {bigger}

Registration Fees

Student: $175 ($200 after May 1st)

Academic/Corporate: $225 ($250 after May 1st)

{/bigger} {/color} {/bold}


Meeting, lodging and travel information may be found at:


{bold} {color} {param} ffff,0000,ffff {/param} {bigger} {bigger} http://usa.hamamat=
su.com/fretflim

{/bigger} {/bigger} {/color} {/bold}

or contact


Victoria Centonze Frohlich (mailto:frohlich-at-uthscsa.edu)


=09





From daemon Thu Apr 5 09:38:34 2001



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 5 Apr 2001 07:32:29 -0700
Subject: RE: digital camera for my Axiolab Zeiss microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kalvoda Jiri writes ...

} Dear colleagues, I would like to buy a digital camera
} for my Axiolab Zeiss microscope.
} Unfortunately I am a bit confused in the amount of
} available data. I would need a digital camera of the
} resolution that matches the quality of film cameras
} in order I need not scan photos or negatives.
} ...

To give you an idea of what you are asking: For comparable
resolution, the camera would need deliver more than 6M pixels ... and
there is also the question of a digital camera capturing the gamut of
color capable of film. For example, the camera you mention, which is
aimed at consumers, probably delivers a gamut aimed at the "sRGB"
color space. Only a film scanner can capture a color gamut comparable
to "Ektaspace RGB".
Still, your camera is likely to do a very good job if properly
adapted to the microscope. You will need a 1X C-mount adapter for the
microscope head, and an adapter for mounting the camera on the
C-mount. These are readily obtained for Nikon Coolpix cameras,
possibly yours too.

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu Apr 5 09:41:41 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Thu, 05 Apr 2001 09:37:45 -0500
Subject: Re: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tina,
I have the scanner you are looking at & like it a lot. To be quite honest I
do not find that I need to exploit it'll full capably. If I were in the market
again, looking at newer technology I would be interested in a faster scanner of
similar quality. Yes I want my cake & to eat it too :).
I'll give you this analogy. If I have 10 negatives I will franchise my time,
that is let things scan while I hang out in the office doing other things. If I
have 20 negatives, I'll probably goto the darkroom to make photos. It is quicker
& paper is cheaper. BTW I have an Epson 870 inkjet that produces nice quality
images... cost is down to $180 US, (now the Epson 880)....no financial interest
in these companies.

Oh yea, get the fastest computer you can afford.

good luck,
Bruce Brinson
Rice U.

Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, All-
}
} A colleague has asked for recommendations for setting up a digital
} darkroom (fun to spend someone else's money!). This person would benefit
} from a really good scanner that could deal with prints, large format
} negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
} into an Agfa Duoscan T2500. Do any of you have an opinion about this or
} other suitable scanners?
}
} I know this subject comes up regularly, but I don't feel bad about
} introducing it again, since technology evolves so quickly!
}
} Mahalo,
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************



From daemon Thu Apr 5 11:49:19 2001



From: John C. Gilkey :      jgilkey-at-u.arizona.edu
Date: Thu, 5 Apr 2001 09:47:02 -0700
Subject: Re: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ...or other suitable scanners?...

Tina,
Have your colleague check out the Imacon Flextight Precision II
scanner. The optical resolution is 5760 dpi for slide-sized objects;
I believe it drops to 4800 dpi for objects the size of her larger
negatives. The scanner collects 14 bits of usable data per channel,
which can be exported as a two bytes per channel, and has a dynamic
range of 3.9 OD units (4.1 OD max). The machine is also very fast.
The URL is:

http://www.imacon.dk/usr/imacon/wppImacon.nsf/pages/flexprecision.html

John


From daemon Thu Apr 5 12:22:25 2001



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 05 Apr 2001 10:18:14 -0700
Subject: Re: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 05:22 PM 4/4/2001 -1000, Tina Carvalho wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Tina,

I have the Duoscan and a Nikon slide scanner. The Duoscan can scan slides
on the special tray feature but side by side comparisons of the Duoscan and
Nikon show that the Nikon scan is much better. For the larger negs we had
a special tray made for the Duoscan and we scan in our EM negs. The Nikon
has gotten much cheaper and an excellent scanner can be had for $700 with
Digital ICE, something you want.

Get two scanners.




Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Apr 5 12:25:53 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Thu, 5 Apr 2001 10:30:06 -0500
Subject: Johns formar coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I forgot to mention John that we do not use formar coated grids. For better
assessment of the renal biopsy we do not mince the sample, either. Please
see our Web page for more details.
{pathology.lsuhsc.edu/Pathist/dx_home.htlm} click on M.diagnostic service
and then on renal biopsy.
Our diagnostic em lab continues to send poloroid HRLM pictures and TEM B&W
contact prints with each report.




From daemon Thu Apr 5 13:20:17 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 05 Apr 2001 14:15:06 -0400
Subject: Microscopy technician sought

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Electron Microscopy
Lehigh University

Lehigh University seeks an Electron Microscopy Technician to perform
duties in support of the Microscopy Center of the Materials Science and
Engineering Department. The person appointed will work with other
technical staff to instruct students in the operation of microscopes and
other equipment; maintain and repair instruments; carry out upkeep of
the lab; support research professors and students; analyze samples; give
tours and demonstrations; maintain a safe environment and perform other
assigned duties. A bachelor's degree in physical science and/or 4+
years related work experience is required. Candidates should be
familiar with electron microscopes, mechanical and electronic equipment,
vacuum systems, computers (PC and/or Mac) and EDS/WDS systems.
Experience with a microprobe would be especially valuable. Good
communication and interpersonal skills are essential.

Lehigh University offers excellent benefits including medical, vision
and tuition. Interested candidates should forward their resume to
Jennifer Mohney, Human Resources, 428 Brodhead Avenue, Lehigh
University, Bethlehem PA 18015. EEO/AA

--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Thu Apr 5 14:28:47 2001



From: Ron L'Herault :      lherault-at-bu.edu
Date: Thu, 5 Apr 2001 15:22:41 -0400
Subject: DNA Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of our students is trying to stain the DNA of osteoblasts using
bisBenzamid, Hoechst no. 33258 trihydrochloride. She is finding conflicting
information about the concentration to use and the lethal dose.

I would appreciate it if someone can provide or point us to a protocol they
have used successfully. We are primarily a materials lab and do not have a
lot of biological reference material at hand.

Thanks.

Ron L



From daemon Thu Apr 5 15:33:49 2001



From: Ron L'Herault :      lherault-at-bu.edu
Date: Thu, 5 Apr 2001 16:28:49 -0400
Subject: Light Microscopy-need DNA stain help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of our students is trying to stain the DNA of osteoblasts using
bisBenzamid, Hoechst no. 33258 trihydrochloride. She is finding conflicting
information about the concentration to use and the lethal dose.

I would appreciate it if someone can provide or point us to a protocol they
have used successfully. We are primarily a materials lab and do not have a
lot of biological reference material at hand.

Thanks.

Ron L






From daemon Thu Apr 5 20:14:12 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 5 Apr 2001 15:08:37 -1000 (HST)
Subject: More digital darkroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, again-

I was initially hesitant to introduce a subject that gets periodically
posted here, but received so many enthusiastic messages about print and
negative scanners that I thought I'd continue the thought. I will be happy
to summarize the responses and throw in some opinions of my own. As more
and more of us convert to "digital darkrooms" we can share more of our
experiences with hardware and software.

I am helping a former traditional photographic media user/computerphobe
set up digital imaging capabilities since her university/museum department
is closing their darkroom facilities and reassigning personnel (sigh). She
has what appears to be a decent budget (until I started pricing the good
stuff!). I am proposing she get a fast (733MHz) G4 Mac with maximum
(1.5GB) RAM, and she saw and fell in love with the Apple 22" cinema
display (as did I when I saw it in person). People seem to like
the Agfa T2500 scanner for prints and negatives. A moderate color printer,
since she has access to other really good printers in her department. A
Nikon Coolpix 990 digital camera for on- and off-microscope. Photoshop
6.0, for which I'll train her. Corel Draw for vector graphics?

Additions, subtractions and comments will be welcome. I'll summarize after
a reasonable amount of time and we'll see if there is a consensus on the
ideal digital darkroom!

Mahalo,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Thu Apr 5 20:31:18 2001



From: Michele von Turkovich :      mvonturk-at-zoo.uvm.edu
Date: Thu, 5 Apr 2001 20:30:30 -0500
Subject: Waste osmium tetroxide storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How does your institution handle short term storage in the lab of waste
osmium tetroxide. Does it need to be in a fume hood? We keep it in closed
glass containers mixed with vegetable oil. Does anyone have a reference
regarding whether or not it needs to be stored in a hood? Thank you for any
responses.




From daemon Thu Apr 5 20:34:40 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Thu, 5 Apr 2001 20:34:22 -0500
Subject: Johns formar coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John, I forgot to mention that we do not use formar coated grids. For better
assessment of the renal biopsy we do not mince the sample, either. Please
see our Web page for more details.
{pathology.lsuhsc.edu/Pathist/dx_home.htlm} click on M.diagnostic service
and then on renal biopsy.
Our diagnostic em lab continues to send poloroid HRLM pictures and TEM B&W
contact prints with each report.




From daemon Thu Apr 5 20:37:27 2001



From: Scott Johnson :      sjohnson-at-brookdale.cc.nj.us
Date: Thu, 5 Apr 2001 20:37:04 -0500
Subject: Grids for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am a laboratory instructor at Brookdale Community College in Lincroft, NJ
who operates a Zeiss 9C TEM which was donated to the college. We are still
in the process of being able to make our own grids. Does anyone have some
grids that they would be willing to sell or donate to the College? Grids
of anything would be greatly appreciated, but basic cellular structures is
really what we are looking for as we are a community college and only have
first and second year students. Thanks in advance for your help.


Scott Johnson




From daemon Fri Apr 6 02:10:51 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Fri, 6 Apr 2001 08:56:06 +0200
Subject: Re:Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina,

I used Agfa DuoScan HiD earlier and I try to get it here as well. I like that
machine a lot. It's optical resolution is 1000x2000 Dynamic range is 3.7D,
which would help scanning DP's. If you want more info you can have a look at:

http://www.agfa.com/scanners/duoscan_HiD.html

Printing is another task you can buy things from AGFA as well. Their
photoprinter is just excellent, but a bit expensive. I have tried nice HP
injet printers with great success.

Cheers!
Csaba

--
____________________________________________
Csaba Cserhati
Univ.of Debrecen / Dept. of Solid State Phys.
Hungary
tel/fax: 36 52 316073
e-mail: cserhati-at-delfin.klte.hu
____________________________________________


From daemon Fri Apr 6 03:08:07 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Fri, 6 Apr 2001 10:04:47 +0200
Subject: Re:Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina,

I used Agfa DuoScan HiD earlier and I try to get it here as well. I like that
machine a lot. It's optical resolution is 1000x2000 Dynamic range is 3.7D,
which would help scanning DP's. If you want more info you can have a look at:

http://www.agfa.com/scanners/duoscan_HiD.html

Printing is another task you can buy things from AGFA as well. Their
photoprinter is just excellent, but a bit expensive. I have tried nice HP
injet printers with great success.

Cheers!
Csaba


--
____________________________________________
Csaba Cserhati
Univ.of Debrecen / Dept. of Solid State Phys.
Hungary
tel/fax: 36 52 316073
e-mail: cserhati-at-delfin.klte.hu
____________________________________________


From daemon Fri Apr 6 03:24:27 2001



From: Csaba Cserhati :      cserhati-at-delfin.klte.hu
Date: Fri, 6 Apr 2001 10:19:37 +0200
Subject: RE: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina,

I used Agfa DuoScan HiD earlier and I try to get it here as well. I like that
machine a lot. It's optical resolution is 1000x2000 Dynamic range is 3.7D,
which would help scanning DP's. If you want more info you can have a look at:

http://www.agfa.com/scanners/duoscan_HiD.html

Printing is another task you can buy things from AGFA as well. Their
photoprinter is just excellent, but a bit expensive. I have tried nice HP
injet printers with great success.

Cheers!
Csaba

--
____________________________________________
Csaba Cserhati
Univ.of Debrecen / Dept. of Solid State Phys.
Hungary
tel/fax: 36 52 316073
e-mail: cserhati-at-delfin.klte.hu
____________________________________________


From daemon Fri Apr 6 03:55:46 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 6 Apr 2001 09:51:46 +0100 (GMT Daylight Time)
Subject: Re: Waste osmium tetroxide storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Based on historical precedent we keep used osmium tetroxide
in the fridge in a "Kilner" jar (for pickled fruit and
veg.), which has a rubber seal.

BTW what ratio of vegetable oil to osmium solution do you
use?

Dave


On Thu, 5 Apr 2001 20:30:30 -0500 Michele von Turkovich
{mvonturk-at-zoo.uvm.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} How does your institution handle short term storage in the lab of waste
} osmium tetroxide. Does it need to be in a fume hood? We keep it in closed
} glass containers mixed with vegetable oil. Does anyone have a reference
} regarding whether or not it needs to be stored in a hood? Thank you for any
} responses.
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"





From daemon Fri Apr 6 08:13:44 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 06 Apr 2001 09:04:16 -0400
Subject: Re: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

In respose to Tina's post, I have not seen any mention on the list of the
scanner I purchased a few weeks ago, the Epson Expression 1640XL. It has
1600dpi optical resolution (scans at a hardware resolution of 1600x3200
dpi) 42 bit color (14 bit gray) and Dmax of 3.6. It is large format, and
the transparency adapter comes with a range of negative holders. Has SCSI
or USB interfaces with firewire as an optional extra (I use USB on a Win
2000 system). Of course, you pay for what you get - it isn't cheap.

We are only just beginning to learn how best to use all the resolution and
bit depth we now have, but I and my users love it!

This is not a comparison, of course (I haven't used the other models) but
just to say we are happy with what we have.

Tony.

}
} Hi, All-
}
} A colleague has asked for recommendations for setting up a digital
} darkroom (fun to spend someone else's money!). This person would benefit
} from a really good scanner that could deal with prints, large format
} negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
} into an Agfa Duoscan T2500. Do any of you have an opinion about this or
} other suitable scanners?
}
} I know this subject comes up regularly, but I don't feel bad about
} introducing it again, since technology evolves so quickly!
}
} Mahalo,
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}

* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Fri Apr 6 08:14:33 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 6 Apr 2001 23:12:44 +1000
Subject: RE: Waste osmium tetroxide storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hysterical precedents aside. The idea of the oil is to absorb all tetroxide
vapours and render the substance harmless. Metallic Os is essentially
non-toxic, so the treated tetroxide waste should smell of whatever vegetable
oil and not emit any of the musky smell emitted by osmium tetroxide.
Neither should the treated material be regarded as hazardous waste - but I
expect that no safety officer would have the courage to make that declaration.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, April 06, 2001 6:52 PM, Patton, David [SMTP:David.Patton-at-uwe.ac.uk]
wrote:
}
}
}
} Based on historical precedent we keep used osmium tetroxide
} in the fridge in a "Kilner" jar (for pickled fruit and
} veg.), which has a rubber seal.
}
} BTW what ratio of vegetable oil to osmium solution do you
} use?
}
} Dave
}
}
} On Thu, 5 Apr 2001 20:30:30 -0500 Michele von Turkovich
} {mvonturk-at-zoo.uvm.edu} wrote:
}
} }
} }
} } How does your institution handle short term storage in the lab of waste
} } osmium tetroxide. Does it need to be in a fume hood? We keep it in closed
} } glass containers mixed with vegetable oil. Does anyone have a reference
} } regarding whether or not it needs to be stored in a hood? Thank you for any
} } responses.
} }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}
}



From daemon Fri Apr 6 08:49:35 2001



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Fri, 06 Apr 2001 09:03:01 -0500
Subject: seeking databases that list distances

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


between membranes in all cellular components that have two or more
membrances and

also seeking "volume and size" of all known subcellular organelles
with or without membranes.
also seeking " other physical measurement parameters" of cellular
components by cell type..
by cell age..
etc.
thanks.

Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find
the cure for cancer and other diseases. There will always be a need for
the trained clinician (MD/RN) but, advanced diagnostic and treatment option
selection has become gene based, has moved from the physician's practice to
the computerized cell and molecular biology laboratory, and appropriate
treatment options should now be based on the personal biology of the
patient.


From daemon Fri Apr 6 08:49:35 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 6 Apr 2001 08:46:54 -0500
Subject: Re: More digital darkroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Adobe Illustrator or Freehand for vector graphics. Corel Draw is less
common and has a proprietary file format that has caused me troubles
preparing articles for Microscopy Today. Corel can save in other
formats, but people have to use that option.
Also, Adobe has educational pricing, and special package prices that
bundle full versions of Photoshop and Illustrator.

Phil

} Hi, again-
}
} I was initially hesitant to introduce a subject that gets periodically
} posted here, but received so many enthusiastic messages about print and
} negative scanners that I thought I'd continue the thought. I will be happy
} to summarize the responses and throw in some opinions of my own. As more
} and more of us convert to "digital darkrooms" we can share more of our
} experiences with hardware and software.
}
} I am helping a former traditional photographic media user/computerphobe
} set up digital imaging capabilities since her university/museum department
} is closing their darkroom facilities and reassigning personnel (sigh). She
} has what appears to be a decent budget (until I started pricing the good
} stuff!). I am proposing she get a fast (733MHz) G4 Mac with maximum
} (1.5GB) RAM, and she saw and fell in love with the Apple 22" cinema
} display (as did I when I saw it in person). People seem to like
} the Agfa T2500 scanner for prints and negatives. A moderate color printer,
} since she has access to other really good printers in her department. A
} Nikon Coolpix 990 digital camera for on- and off-microscope. Photoshop
} 6.0, for which I'll train her. Corel Draw for vector graphics?
}
} Additions, subtractions and comments will be welcome. I'll summarize after
} a reasonable amount of time and we'll see if there is a consensus on the
} ideal digital darkroom!
}
} Mahalo,
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Apr 6 08:51:11 2001



From: Sara Miller :      saram-at-duke.edu
Date: Fri, 6 Apr 2001 09:45:29 -0400 (EDT)
Subject: Re: Waste osmium tetroxide storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We keep used osmium in small sealed bottles in the hood. Our
environmentals services office would rather have **undiluted/unmixed** (no
oil) waste for them to neutralize. They have no way of knowing whether
all the osmium has been denatured by attachment to oil and would have to
treat the whole bottle as osmium waste making for more volume to have to
dispose of. Check with your hazardous waste office.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Apr 6 08:51:36 2001



From: inikolak-at-mred.tuc.gr.or.eisodos-at-otenet (by way of Nestor J. Zaluzec)
Date: Fri, 6 Apr 2001 08:51:08 -0500
Subject: Ask-A-Microscopist: enumeration of airborne microorganisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: inikolak-at-mred.tuc.gr or eisodos-at-otenet.
Name: Irene Nikolakaki

Organization: Technical University of Crete

Education: Graduate College

Location: Chanea, Crete,Greece

Question: Dear sir/madam,

I am a postgraduate student at the Technical University of Crete and
I seek information (as detailed as possible) on how enumeration of
airborne microorganisms collected on Nuclepore filters is done with
the use of epi-fluorescence microscopy.I would be grateful if you
could provide me with this infromation or any kind of help as to
where I can find it.

Sincerely yours,
Irene Nikolakaki






---------------------------------------------------------------------------


From daemon Fri Apr 6 09:46:57 2001



From: rgriffin-at-eng.uab.edu
Date: Fri, 6 Apr 2001 09:41:58 -0500
Subject: More digital darkroom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I both run the microscope labs here and am a researcher in materials
engineering.

First I want to say that I strongly support the use of the digital
laboratory. While we still occasionally use film for our highest quality
requirements, in general we are fully digital. The use of digital cameras
has really expanded our undergraduate teaching laboratories and has sped up
our research.

I have found one "dark-side" to a digital imaging laboratory as a lab
manager. As the lab manager, I have found that keeping a digital laboratory
up-to-date is much more expensive than the film laboratory. When we were
only film, we had to repair the film cartridges for our Polaroid PN film (it
takes about five minutes) and have the microscopes cleaned about once a year
at a cost of about $1k.

The digital lab. is much more expensive time and repair wise. Because we
crunch our computers with our image size and storage, it takes more of my
time to keep stuff going. All our computers are networked and in addition
to work, the students tend to junk up the computers with downloads etc which
stops them from working for the image processing work. This requires
continual monitoring on my part (in spite of rules against using them for
these applications!) In addition, keeping computers that will run the data
is expensive. I buy pretty much the best out there, but somehow upgrades
are still inevitable. I also have to supply print cartridges,etc.
Researchers always supplied their own film and dark room supplies. In
addition, I've had to have our cameras repaired numerous times. The cost
was high (at least $500) and they stayed gone for up to a month. Finally,
some of my cameras are about 3 years old. I can see a degredation in the
image quality from when they were purchased. The cameras are much noisier.
I see a future of regular replacement of my cameras in addition to the
computer upgrades. So while the cost to the researchers is lower (which
helps me as a researcher), the cost to the lab itself is higher (which hurts
me as a lab manager). I'm working on setting up a fee schedule for this
equipment but REALLY hate to have to do it. All of you who do this in a
university know how painful it is!

Regarding the camera purchase-in addition to considering the camera
resolution and cost, I think you should consider the image transfer. I
recommend considering a camera with immediate transfer of the image to the
microscope if you have numerous inexperienced users. Being able to focus on
the screen is extremely helpful. The image transfer time is also important
if you have many images to capture. We do image analysis on numerous images
and some of the cameras have about a 30 second transfer time for decent
resolution. This would be unbearable for the number of images we collect.
I'm not sure how the Nikon Coolpix works but this should be considered by
anyone that is purchasing a digital camera.

Good luck!

Robin Griffin
UAB

-----Original Message-----
} From: Tina Carvalho [mailto:tina-at-pbrc.hawaii.edu]
Sent: Thursday, April 05, 2001 6:09 PM
To: Microscopy Listserver


Hi, again-

I was initially hesitant to introduce a subject that gets periodically
posted here, but received so many enthusiastic messages about print and
negative scanners that I thought I'd continue the thought. I will be happy
to summarize the responses and throw in some opinions of my own. As more
and more of us convert to "digital darkrooms" we can share more of our
experiences with hardware and software.

I am helping a former traditional photographic media user/computerphobe
set up digital imaging capabilities since her university/museum department
is closing their darkroom facilities and reassigning personnel (sigh). She
has what appears to be a decent budget (until I started pricing the good
stuff!). I am proposing she get a fast (733MHz) G4 Mac with maximum
(1.5GB) RAM, and she saw and fell in love with the Apple 22" cinema
display (as did I when I saw it in person). People seem to like
the Agfa T2500 scanner for prints and negatives. A moderate color printer,
since she has access to other really good printers in her department. A
Nikon Coolpix 990 digital camera for on- and off-microscope. Photoshop
6.0, for which I'll train her. Corel Draw for vector graphics?

Additions, subtractions and comments will be welcome. I'll summarize after
a reasonable amount of time and we'll see if there is a consensus on the
ideal digital darkroom!

Mahalo,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *

* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*

****************************************************************************




From daemon Fri Apr 6 10:37:58 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 6 Apr 2001 08:33:30 -0700 (PDT)
Subject: Microscopy and Microanalysis cover picture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
On the September/October issue of Microscopy and Microanalysis, they have
a cover picture of a yeast SEM image collected by David Scharf. I was
wondering if anyone knew the details of how the sample was prepared. It
looks as though the sample was processed directly from the medium it was
growing on. I am used to processing bacteria and yeast by filtering
through membrane filters, or depositing on polylysine treated
glass/silica. I was wondering if there was something different done for
this sample?

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Fri Apr 6 10:37:58 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Fri, 6 Apr 2001 11:35:54 -0400
Subject: Sputter coater questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in need of some form of a conductive film coater for SEM sample
preparation. I have found a Tousimis Research Corporation Samsputter-2a
sputter coater, though it may not be working properly.

Until now, I have been considering constructing my own sputter coater from
microwave oven parts and the like, but I have not yet found any books or
other resources with clear enough information to get my confidence to the
point that I feel that I can do it. I have an understanding of what is
needed electrically, but I need something that fills in some details like
"Be sure that the target to sample distance can be adjusted between X and Y.
You need to get a QRZ type needle valve to admit the argon", etc.

Does anyone have experience with the Tousimis Samsputter 2a? From what I can
see, it is an extremely simple unit. Is it something that would be worth
repairing? Would it best be used to scavenge parts for a home-built unit?

Since I have breeched the question of home-built sputter coaters, does
anyone know of any resources that describe the dos and donts of building
one? I know that there are a number of vendors on the listserv. What are the
critical features of your units that I just can't get in a home-built unit
and just can't live without?

To put this all in perspective, I have an old SEM with 100 Angstrom
resolution at best. I don't need flawless, grainless deposition. I have
close to zero budget. Even a used sputter coater at market prices is far too
expensive. Either I make something like this work, or I learn to work with
uncoated samples (which will be difficult, since I am interested in looking
at clays - small particle size with next to zero conductivity).

Bruce "SEM on a shoestring" Girrell





From daemon Fri Apr 6 10:40:25 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 6 Apr 2001 10:37:00 -0500
Subject: Preparation of a steel wedge on a ceramic substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest to use SEM (with calibrated magnification) if
you could electroplate your sample with layer of Ni 0.1-0.3 mm thick.
Sorry, I do not have a protocol for plating with Ni now, but I did
it many times and it is pretty easy.

After coating with Ni you can cut your sample, mount it in epoxy or
thermoset and prepare usual cross section. Ni would save the edge of steel
layer and measurements will be very simple.

If surface of you samples is flat, you can cut them (better with
diamond saw), mount them in epoxy with a steel strip (instead of Ni)
attached to steel layer and polish. It is less dependable but still not bad
method.

Vladimir Dusevich

-----Original Message-----
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
[mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com]
Sent: Thursday, April 05, 2001 8:25 AM
To: microscopy-at-sparc5.microscopy.com
Cc: hban-at-eng.uab.edu


We have a professor here who has a 1 cm x 2.5cm steel layer about 100 um in
thickness on a ceramic substrate. The metal layer was sputter deposited
onto the ceramic substrate. The ceramic substrate extends past the metal
layer. He needs to get a thickness gradient across the steel layer along
the 2.5 cm length. He would like to have one end at about 50 microns in
thickness and the other at 2 microns with a gradually decreasing thickness
gradient. Steps down would be ok although a smooth transition would be
better. We have a laser profilometer to measure anything that we produce.

Does anyone out there have any ideas how to do this? Would a tripod
polisher work? I thought about electropolishing and masking off portions at
a time but I worry about what will happen at the interface between the
ceramic and the metal. Could we alter a dimpler?



Thanks,

Robin Griffin
UAB


From daemon Fri Apr 6 11:50:20 2001



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Fri, 06 Apr 2001 09:44:40 -0700
Subject: RE: Osmium waste urban legend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Osmium waste is toxic and hazardous due to its reactivity and heavy metal characteristics. One of the "urban legends" that abounds is that Osmium mixed with oil is "neutralized" and made safe. It is true that much of it is reduced to metallic Osmium. However in a bizarre incident it was shown in our lab years ago that reduced Osmium is very reactive with strong oxidizers. In the incident some reduced Osmium was accidentally spilled in a sink which had a small amount of Hydrogen Peroxide in the drain. The ensuing yellow gas cloud of newly formed Osmium Tetroxide from the exothermic reaction was very toxic!!!! I mention this only to confirm that Sara Miller is right again as usual and that mixing Osmium waste with other compounds does not make it safe and does increase the volume.



From daemon Fri Apr 6 15:30:09 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Fri, 06 Apr 2001 15:26:46 -0500
Subject: GMA recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I've been tearing hair out all day trying to find a recipe for GMA. In the
past, I have used either JB-4 or Historesin (or Technovit) embedding kits,
which have clear directions for embedding tissue and polymerizing these
resins without UV. I recently ordered a straight GMA embedding kit (because
it was much cheaper), and received not one bit of direction as to how I
should combine the three components. I did find a protocol on EMS' website
for UV polymerization, but would much prefer to use the non-UV method,
since it allows tissue to be better oriented. Before I start experimenting,
I thought I'd see if anyone out there can help.
Thanks again for your help.
Bald in Iowa,
Kristen
Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu



From daemon Fri Apr 6 17:41:09 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 6 Apr 2001 23:37:57 +0100
Subject: Osmium waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Osmium metal is sometimes reported not to react with air, but other
sources report slow reactivity of the metal with air to produce OsO4.
It is interesting to note that the name osmium is derived from the
Greek "osme" meaning smell, referring to the odour of the metal
resulting from osmium tetroxide production at its surface. Presumably
neither the finely divided metal nor the dioxide (osmium black) can be
trusted not to oxidise to OsO4 in air. What makes osmium tetroxide
especially hazardous is the fact that it is very volatile. Unlike
OsO4 neither osmium metal nor osmium dioxide are volatile, and
provided oxygen can be prevented from reaching them they are therefore
relatively innocuous. Surrounding them in oil is probably therefore a
good strategy, provided the mixture is still treated as toxic waste.


Dr. Chris Jeffree
Inveresk Cottage
26, Carberry Road
Inveresk
Musselburgh
Midlothian
EH21 8PR
Tel: +44 131 665 6062
FAX +44 131 653 6248
Mobile 07710 585 401



From daemon Fri Apr 6 19:53:46 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Fri, 6 Apr 2001 20:47:56 -0400
Subject: Preparation of a steel wedge on a ceramic substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that I have missed part of this message string somewhere, but it
sounds like you are starting with a uniform thickness steel layer that has
been deposited on a ceramic substrate. I will then make the assumption that
you are attempting to alter the thickness of the steel layer across the
length of the sample to produce a 'wedge'. I am not sure that this will
help, but you could try the wedge polishing technique used for TEM sample
preparation, or bevel polishing. I have had success bevel polishing IC's,
but not to the degree of accuracy that you require. I know that South Bay
technologies makes a pretty good tripod with micrometer levels at all three
corners that may help you out. The biggest problem will be in setting up to
ensure a good gradient. You should probably try it a few times with 'dummy'
samples to get the feel for how aggressive your polish angle is. I hope this
helps.
Nick Aitken

-----Original Message-----
} From: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
Sent: Friday, April 06, 2001 11:37 AM
To: microscopy-at-sparc5.microscopy.com
Cc: hban-at-eng.uab.edu


I suggest to use SEM (with calibrated magnification) if
you could electroplate your sample with layer of Ni 0.1-0.3 mm thick.
Sorry, I do not have a protocol for plating with Ni now, but I did
it many times and it is pretty easy.

After coating with Ni you can cut your sample, mount it in epoxy or
thermoset and prepare usual cross section. Ni would save the edge of steel
layer and measurements will be very simple.

If surface of you samples is flat, you can cut them (better with
diamond saw), mount them in epoxy with a steel strip (instead of Ni)
attached to steel layer and polish. It is less dependable but still not bad
method.

Vladimir Dusevich

-----Original Message-----
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
[mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com]
Sent: Thursday, April 05, 2001 8:25 AM
To: microscopy-at-sparc5.microscopy.com
Cc: hban-at-eng.uab.edu


We have a professor here who has a 1 cm x 2.5cm steel layer about 100 um in
thickness on a ceramic substrate. The metal layer was sputter deposited
onto the ceramic substrate. The ceramic substrate extends past the metal
layer. He needs to get a thickness gradient across the steel layer along
the 2.5 cm length. He would like to have one end at about 50 microns in
thickness and the other at 2 microns with a gradually decreasing thickness
gradient. Steps down would be ok although a smooth transition would be
better. We have a laser profilometer to measure anything that we produce.

Does anyone out there have any ideas how to do this? Would a tripod
polisher work? I thought about electropolishing and masking off portions at
a time but I worry about what will happen at the interface between the
ceramic and the metal. Could we alter a dimpler?



Thanks,

Robin Griffin
UAB


From daemon Sun Apr 8 02:31:51 2001



From: thespyisnow23-at-ozbytes.net.au
Date: Sat, 07 Apr 2001 20:54:14 -0700
Subject: NEED A MORTGAGE LOAN?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Sun Apr 8 04:42:27 2001



From: thespyisnow23-at-ozbytes.net.au
Date: Sat, 07 Apr 2001 20:54:14 -0700
Subject: NEED A MORTGAGE LOAN?

Contents Retrieved from Microscopy Listserver Archives
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{HTML}
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{FONT face=3D"MS Sans Serif"}
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From daemon Sun Apr 8 12:53:25 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 08 Apr 2001 13:45:11 -0500
Subject: OsO4 storage/recycling

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:
==================================================
Hysterical precedents aside. The idea of the oil is to absorb all tetroxide
vapours and render the substance harmless. Metallic Os is essentially
non-toxic, so the treated tetroxide waste should smell of whatever
vegetable
oil and not emit any of the musky smell emitted by osmium tetroxide.
Neither should the treated material be regarded as hazardous waste - but I
expect that no safety officer would have the courage to make that
declaration.
==================================================
Jim might be right but there are other points of view on this:

1] I have been led to believe that the vegetable oil reduction of remaining
unreduced tetroxide reduces things down to the dioxide, not the metal. The
dioxide is a black colloidal solid (the metal is a lighter "bluish gray").
Osmium dioxide is itself relatively innocuous, for example, it is not
"regulated" as a dangerous good when shipped, either domestically under US
DOT rules or internationally under IATA rules.

2] Osmium is a non-renewable resource and as such, we should all be looking
for ways to recycle such materials as opposed to taking them to landfills or
in the case of osmium, incineration. I am no environmental "activist"
myself, but I think we should all be thinking about such things. The
possibility of recycling something however, is intimately connected with the
economics of recycling vs. the cost of the purchase of new virgin material.
And when the "used" osmium tetroxide containing aqueous liquid is
"neutralized" in vegetable oil, for those involved in precious metals
recycling, this act essentially kills the economics of recycling.

3] We have in beta testing stage right now an "osmium recycling kit". We
are looking for a limited number of laboratories (for now, just in the USA)
to participate in our beta testing of this kit. If you are interested in
participating in this test, let me know off-line and I will send you the
details.

4] In the mean time, I would offer the following advice. Consider using
one of the other methods described on this listserver in the past, such as
the ones involving KOH as a reducing agent. The reduced material in this
state can be recycled economically, but only in large quantities. No one
laboratory, in our opinion, could generate enough such material over a
reasonable period of time, to make recycling and refining, even of the
material is in this state, economical.

5] The one thing you don't ever want to do, at least in the USA, is to
declare this as any kind of a "hazardous waste". Once something has been
declared to be a hazardous waste, that designation can never (if my
understanding of regulations is correct) be reversed, and it forever has to
be treated as a waste, and translated, that means it is destined for
eventual disposal by either landfill or incineration. I want to be very
careful here, it is complicated, this is true so long as we are talking
about a RCRA hazardous waste, that is, it meets a listing or characteristic
definition. One environmental experts tells me the following: "The reason
OsO4 (and OsO2, for that matter) are not regulated as hazardous has nothing
to do with their human toxicity (or lack of); it is because osmium, from a
regulatory standpoint, is not considered toxic to the environment." He
also says it is OK to designate something as a hazardous waste with the
intent to recycle it; it simply must be managed as a hazardous waste while
it is on-site.

So these containers that are holding reduced material (from the tetroxide)
for recycling must be labeled properly, and that would mean something like
"osmium dioxide for recycling". I am getting into an area that is not black
and white defined, and probably varies from institution to institution in
the US, not to mention the variation from country to country. But the
important thing is that from a regulatory point of view, what that label
says ends up determining the possibilities for the ultimate fate of its
contents.

We are striving to find a way to help people transfer, on their
environmental "accounting sheets", a material from the column saying
"incineration" or "landfill", to the "recylcing" column. And for those who
worry about such things, from a legal liability standpoint, there is general
recognition that if the material is recycled, there is far less legal
liability associated with its disposal than if it is incinerated or sent to
landfill. And at the same time, recycling keeps this most valuable
nonrenewable resource in the stream of commerce and available for future
generations.

Disclaimer: SPI Supplies has developed a kit for recycling osmium. It is
not yet commercially available, but is in beta test stage. We also have the
obviously ulterior motive of making sure there is osmium tetroxide available
for future generations of EM users, otherwise there would be no place for
firms like SPI Supplies.......

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Mon Apr 9 00:17:11 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Mon, 9 Apr 2001 15:12:22 +1000
Subject: RE: OsO4 storage/recycling

Contents Retrieved from Microscopy Listserver Archives
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Different people approach such subjects from rather different angles and they
may all be right, but considering their own realities.

A couple of submissions approached the subject from an "absolute safety" point
of view. I don't subscribe to that because the amount of tetroxide emitted from
gram quantities of metal and some dioxide when surrounded by the tetroxide
absorbing oil, would be miniscule. In my opinion, Os waste in vegetable oil is
not a substantial hazard in the lab or when dumped. For many, particularly in
the USA this is irrelevant, because of strict legal obligations.

There is also an environmental angle. Unfortunately, greatest safety regardless
of cost, is at odds with good environmental practice. If a lot of material and
energy is used to dispose of a low hazard material, then the total
environmental cost may be very high when compared with an "acceptable risk"
solution. We should not confuse the ever-increasing demands for greatest safety
with "environmentally friendly" practices: they are not synonymous, but often
mutually exclusive.

I was pleased to read some of Chuck's submission. Whatever the motivation,
recycling of a limited resource is commendable. This has been tried before by
turning the waste material back into the tetroxide. I understand that few
people still practice the regeneration of osmium. One reason is that the actual
fixative solution is poorly defined after reclamation from diverse fixation
vehicles.

Chuck's idea of turning the material into metallic osmium is possible. Using
precision electrolysis, for instance with SS electrodes at ~400volts, with the
plate size determining amperage, the recovered metal could be 99.9% pure. The
refiner, however, would charge for assaying and refining and this means another
business would need to collect the osmium, resulting in double shipping and
double mark-ups.
Considering the few grams recoverable, instrument cost and maintenance, this
would be a marginal business, but one that the larger labs certainly should
consider.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, April 09, 2001 4:45 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com]
wrote:
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
} ==================================================
} Hysterical precedents aside. The idea of the oil is to absorb all tetroxide
} vapours and render the substance harmless. Metallic Os is essentially
} non-toxic, so the treated tetroxide waste should smell of whatever
} vegetable
} oil and not emit any of the musky smell emitted by osmium tetroxide.
} Neither should the treated material be regarded as hazardous waste - but I
} expect that no safety officer would have the courage to make that
} declaration.
} ==================================================
} Jim might be right but there are other points of view on this:
}
} 1] I have been led to believe that the vegetable oil reduction of remaining
} unreduced tetroxide reduces things down to the dioxide, not the metal. The
} dioxide is a black colloidal solid (the metal is a lighter "bluish gray").
} Osmium dioxide is itself relatively innocuous, for example, it is not
} "regulated" as a dangerous good when shipped, either domestically under US
} DOT rules or internationally under IATA rules.
}
} 2] Osmium is a non-renewable resource and as such, we should all be looking
} for ways to recycle such materials as opposed to taking them to landfills or
} in the case of osmium, incineration. I am no environmental "activist"
} myself, but I think we should all be thinking about such things. The
} possibility of recycling something however, is intimately connected with the
} economics of recycling vs. the cost of the purchase of new virgin material.
} And when the "used" osmium tetroxide containing aqueous liquid is
} "neutralized" in vegetable oil, for those involved in precious metals
} recycling, this act essentially kills the economics of recycling.
}
} 3] We have in beta testing stage right now an "osmium recycling kit". We
} are looking for a limited number of laboratories (for now, just in the USA)
} to participate in our beta testing of this kit. If you are interested in
} participating in this test, let me know off-line and I will send you the
} details.
}
} 4] In the mean time, I would offer the following advice. Consider using
} one of the other methods described on this listserver in the past, such as
} the ones involving KOH as a reducing agent. The reduced material in this
} state can be recycled economically, but only in large quantities. No one
} laboratory, in our opinion, could generate enough such material over a
} reasonable period of time, to make recycling and refining, even of the
} material is in this state, economical.
}
} 5] The one thing you don't ever want to do, at least in the USA, is to
} declare this as any kind of a "hazardous waste". Once something has been
} declared to be a hazardous waste, that designation can never (if my
} understanding of regulations is correct) be reversed, and it forever has to
} be treated as a waste, and translated, that means it is destined for
} eventual disposal by either landfill or incineration. I want to be very
} careful here, it is complicated, this is true so long as we are talking
} about a RCRA hazardous waste, that is, it meets a listing or characteristic
} definition. One environmental experts tells me the following: "The reason
} OsO4 (and OsO2, for that matter) are not regulated as hazardous has nothing
} to do with their human toxicity (or lack of); it is because osmium, from a
} regulatory standpoint, is not considered toxic to the environment." He
} also says it is OK to designate something as a hazardous waste with the
} intent to recycle it; it simply must be managed as a hazardous waste while
} it is on-site.
}
} So these containers that are holding reduced material (from the tetroxide)
} for recycling must be labeled properly, and that would mean something like
} "osmium dioxide for recycling". I am getting into an area that is not black
} and white defined, and probably varies from institution to institution in
} the US, not to mention the variation from country to country. But the
} important thing is that from a regulatory point of view, what that label
} says ends up determining the possibilities for the ultimate fate of its
} contents.
}
} We are striving to find a way to help people transfer, on their
} environmental "accounting sheets", a material from the column saying
} "incineration" or "landfill", to the "recylcing" column. And for those who
} worry about such things, from a legal liability standpoint, there is general
} recognition that if the material is recycled, there is far less legal
} liability associated with its disposal than if it is incinerated or sent to
} landfill. And at the same time, recycling keeps this most valuable
} nonrenewable resource in the stream of commerce and available for future
} generations.
}
} Chuck
}


From daemon Mon Apr 9 03:42:14 2001



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Mon, 9 Apr 2001 13:28:08 +0530
Subject: TEM of Cadmium Telluride - need advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a proposal for TEM characterisation of cadmium telluride nanoparticles for morphology and size information. I would appreciate information from the list members whether

1. CdTe and related compounds are stable under electron irradiation
2. there is a accelerating voltage limit to be adhered to
3. any other precautions required to ensure microscope safety

My primary microscopy experience is with metallic alloys and ceramics. I have this impression that these compounds are low melting, unstable and likely to sputter onto the pole pieces. Kindly correct and advise me. I use a JEOL 2000 EX II top entry stage operating at 200 kV.

----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----






From daemon Mon Apr 9 08:07:53 2001



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Mon, 9 Apr 2001 09:01:42 -0400
Subject: TEM of Cadmium Telluride - need advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Divakar,

Years ago, I did CdTe/CdS junction. As far as I can remember, both layers
were beam sensitive and the layer CdS was more so. I could get fairly good
twined CdTe structures as well as its overall morphology, but had difficulty
revealing the details in CdS as the available effective observation time was
much limited plus the CdS layer was very thin. There are a few ways to get
around of the issue, including using a) C-coating, b) small spot size (} 2),
and perhaps c) proper keV's (I was using 100keV, the max available voltage
for me that time). Good luck!

Chao-Ying Ni
Scientist
Rodel Inc.
USA

-----Original Message-----
} From: Divakar R [mailto:divakar-at-igcar.ernet.in]
Sent: Monday, April 09, 2001 3:58 AM
To: Microscopy (E-mail)


There is a proposal for TEM characterisation of cadmium telluride
nanoparticles for morphology and size information. I would appreciate
information from the list members whether

1. CdTe and related compounds are stable under electron irradiation
2. there is a accelerating voltage limit to be adhered to
3. any other precautions required to ensure microscope safety

My primary microscopy experience is with metallic alloys and ceramics. I
have this impression that these compounds are low melting, unstable and
likely to sputter onto the pole pieces. Kindly correct and advise me. I use
a JEOL 2000 EX II top entry stage operating at 200 kV.

----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----






From daemon Mon Apr 9 08:28:46 2001



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Mon, 09 Apr 2001 09:14:46 -0400
Subject: Re: Preparation of a steel wedge on a ceramic substrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin,

The tripod polisher may just work. Although your sample is larger than
anything I have previously tripoded, it should work if you use a plain
L-bracket. First, planarize your sample and the two back feet of the
tripod polisher. Then adjust the two back feet to give you the wedge angle
you desire (just slightly over 1 degree if my trigonometry for your sample
is correct). Likewise, the Multiprep system from Allied High Tech should
be able to accept and wedge polish a sample of this size.

If this approach does not work then you may need to use a precision lapping
and polishing jig. You can either design your own or you may want to check
with South Bay Technology. They make several jigs with precise angular
control for polishing crystals. I don’t know however, if they will work
with samples this large.

Hope this helps.

Eric Windsor

Disclaimer: I have no financial interest in either South Bay Technology or
Allied High Tech Products. I am a satisfied customer of both. Also, there
may be other products on the market that will work equally well for
preparing this sample.

The opinion expressed is my own and not that of my employer (NIST).

Original Message:

We have a professor here who has a 1 cm x 2.5cm steel layer about 100 um in
thickness on a ceramic substrate. The metal layer was sputter deposited
onto the ceramic substrate. The ceramic substrate extends past the metal
layer. He needs to get a thickness gradient across the steel layer along
the 2.5 cm length. He would like to have one end at about 50 microns in
thickness and the other at 2 microns with a gradually decreasing thickness
gradient. Steps down would be ok although a smooth transition would be
better. We have a laser profilometer to measure anything that we produce.
Does anyone out there have any ideas how to do this? Would a tripod
polisher work? I thought about electropolishing and masking off portions at
a time but I worry about what will happen at the interface between the
ceramic and the metal. Could we alter a dimpler?


Thanks,
Robin Griffin
UAB




From daemon Mon Apr 9 09:59:04 2001



From: JHoffpa464-at-aol.com
Date: Mon, 9 Apr 2001 10:53:32 EDT
Subject: rapid renal processing

Contents Retrieved from Microscopy Listserver Archives
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--part1_b9.cf4bf0a.280326ec_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

i am interested in a rapid i.e. same day processing schedule, for diagnostic
} renal bx. it must be reliable and the cutting qualities good, since we do a
} lot of low mag work (250x). thanks in advance.
} john


--part1_b9.cf4bf0a.280326ec_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} i am interested in a rapid i.e. same day processing schedule, for diagnostic
{BR} > renal bx. it must be reliable and the cutting qualities good, since we do a
{BR} > lot of low mag work (250x). thanks in advance.
{BR} > john
{BR} {/FONT} {/HTML}

--part1_b9.cf4bf0a.280326ec_boundary--


From daemon Mon Apr 9 10:25:22 2001



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 9 Apr 2001 08:21:27 -0700
Subject: RE: Scanner for negs and prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony writes ...

} In respose to Tina's post, I have not seen any
} mention on the list of the scanner I purchased
} a few weeks ago, the Epson Expression 1640XL.
} It has 1600dpi optical resolution (scans at
} a hardware resolution of 1600x3200 dpi) 42 bit
} color (14 bit gray) and Dmax of 3.6.

I would certainly believe the resolution and the color depth for this
scanner is adequate, but if scanning TEM films is an issue, I'd
seriously advise measuring the optical density of your films ... I've
heard these approach OD} 4 ... which would imply you might consider the
dedicated film scanners, e.g., Polaroid 45 Ultra or the new Nikon
LS-8000.

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Mon Apr 9 12:14:51 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Mon, 9 Apr 2001 12:10:46 -0500 (CDT)
Subject: Scanners: quantitative accuracy

Contents Retrieved from Microscopy Listserver Archives
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I have been listening to the thread on scanners. Has anyone done
tests of how accurate they are in absolute terms for quantitative
digitization?

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Mon Apr 9 12:57:59 2001



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Mon, 09 Apr 2001 12:52:50 -0500
Subject: cryo-systems for SEM

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I'm looking for companies (other than Gatan whom I've already contacted)
that sell cryo-stage and cryo-prep. systems for a JEOL 5800LV scanning
electron microscope. Thanks for any input!
Tracey


Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Mon Apr 9 13:54:43 2001



From: BOES,TERESA (HP-Corvallis,ex1) :      teresa_boes-at-hp.com
Date: Mon, 9 Apr 2001 11:50:14 -0700
Subject: Substitutes for absolute ethanol?

Contents Retrieved from Microscopy Listserver Archives
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Has anyone used any of the denatured ethanols as a substitute for absolute
ethanol?

We have recently run into some difficulty when needing to reorder 200 proof
ethanol, which we use for dehydration and infiltration of samples (primarily
many types of paper) prior to embedding in Spurrs epoxy, and for cleaning
samples (non paper) and lenses of light microscopes. The chemical company
selling the ethanol is insisting that we must have a liquor license before
they will ship to us.

Ethanol denatured with a variety of substances is readily available and can
be shipped with no licensing requirements. Our concern is that the
denaturing agent will leave a detectable residue on lenses, samples, and may
cause problems with the polymerization of Spurrs. Rather than obtaining a
liquor license, we are considering using one of the 100:5 ethanol: methanol
blends. If any of you have had successful or unsuccessful experiences
substituting denatured ethanol for absolute in embedding or cleaning
protocols, I would appreciate hearing from you.

Teresa Boes
Hewlett-Packard
Analytical and Development Lab
1000 Circle Blvd
Corvallis, OR 97330
541-715-7055
teresa_boes-at-hp.com



From daemon Mon Apr 9 15:52:22 2001



From: eric :      biology-at-ucla.edu
Date: Mon, 09 Apr 2001 13:48:33 -0700
Subject: RE: renal Em

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hiya John,


Sorry about being late with this thread...Out here we do about 750 renal
biopsies last year and all our sections are mounted on 300 mesh uncoated
thin bar Gilder grids. We have a Philips 208S at 80kV. We can get a
majority of the glomerulus to lie in the grid square to be viewed. Most of
our images are shout between 2800X and 14,000X.

Last November we finally obtained the AMT Advantage HR 1K x 1K system and
use it exclusively for our EM images... We also do some Neuropathology
and Surgical Pathology EM in this lab.

Out here we have rigged up a system that all the Pathologists who need
the EM images are setup on a EM users group on the network. I them upload
the images from the computer here in the scope room to a directory on our
network so the Pathologists can view the images right at their
desktop. The renal Pathologist here is thrilled with the system... It
cuts down our expenses, and it shortens our turnaround time on specimens
from 5 days to about 3 days or less....

Eric A. Rosen
Electron Microscopist
UCLA Medical Center


==============================


} John:
} We do around 500 renal biopsies per year and all the sections are
} mounted on 200 mesh uncoated copper grids. We have an 8 year old Hitachi
} 7100 and use 60kv. The majority of the glomerulus can be viewed with the
} 3-4 serial sections lying randomly across the grid bars. We do not need a
} picture of the whole glomerulus, rather most pictures are between 3,000 and
} 10,000X.
} Dr. Tibor Nadasdy is the renal pathologist and decided last year that
} all our renal biopsies would be captured with the digital camera onto a
} computer and sent up to him via a network to his computer. So, at the
} present time we use very little EM film. He diagnoses each biopsy and
} e-mails representative digitized images to the nephrologists.
}
}
}
}
} Karen L. Jensen, M.S.
} Project Manager & Associate Scientist
} Electron Microscopy Research Core
} -----Original Message-----
} } From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Friday, March 30, 2001 2:20 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: renal Em
} ok taking a little survey. i am in a diagnostic EM lab. we mount out
} sections
} on formvar coated slotted grids, so he can shoot pics of the whole
} glomerlus.
} ok my question. how may of you out there doing diagnostis EM on renals do
} this?
} john




From daemon Mon Apr 9 16:05:15 2001



From: Jesse Rodrigues :      Jesse_Rodrigues-at-kopin.com
Date: Mon, 9 Apr 2001 17:39:39 -0400
Subject: Scribe tools

Contents Retrieved from Microscopy Listserver Archives
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Tracey
VG Microtech make Polaron integrated column-mounted cryo systems
including a new system
designed for high-resolution work with FEGSEMs
Emitech have an off-microscope cryo-prep and transfer system suitable
for LTSEM with a conventional tungsten filament or LaB6 SEM
BalTec make various cryo transfer and preparation systems which could
be used to transfer specimens from e.g. a freeze-etcher to SEM

see http://www.kaker.com/mvd/list.html for addresses and contact
numbers for these companies
Chris

----- Original Message -----
} From: "Tracey M. Pepper" {tpepper-at-iastate.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, April 09, 2001 6:52 PM




------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I am looking for a 2" and/or 3" scribe tool for GaN. Does anyone have
any experience with a quality new/used equipment vendor who supplied such a
tool in the past. I am looking for a fairly new programmable scriber.

Thank you,

Jesse Rodrigues
Device Processing Manager
Kopin Corporation
695 Myles Standish Blvd.
Taunton, MA 02780
Ph#(508)824-6696 Fax#(508)824-6958
email: jrodrigues-at-kopin.com




From daemon Mon Apr 9 16:39:46 2001



From: Mark Keller :      mark.keller-at-boulder.nist.gov
Date: Mon, 9 Apr 2001 15:35:24 -0600
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon Apr 9 17:09:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 9 Apr 2001 18:04:31 -0400
Subject: RE: Preparation of a steel wedge on a ceramic substrate

Contents Retrieved from Microscopy Listserver Archives
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Eric,
I think that your geometry is off. The Tripod Polisher is about 50 mm long from the sample to the feet and has 1 um divisions. The arctan of 1/50000 gives about 0.001 degrees. This job needs an angle given by the arctan of 48/25000 or 0.11 degrees. Plus you need to stick the thickness at one end at a particular thickness -2 um. It is doable with the TP, but will be difficult. You are correct that they will need to planarize the sample. What I would do is also planarize the holder and mount the parallel-sided sample and then set the height of the feet to the thickness of the sample and then add the amount for the angle. Then I would slowly polish using a low value grit 1 or 3 (perhaps even 1/2) um until I went through to the substrate at one end. You would have your angle and thickness values that went from zero to the desired value and a little thicker. The trick is stopping at the right place and accounting for the wear on the feet. You should be able to watch the facet
move towards one end. You can watch the progress using a glass plate and look at the thickness fringes at the facet caused by the unpolished and polished surfaces.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--




} -----Original Message-----
} From: Eric Windsor [mailto:Eric.Windsor-at-nist.gov]
} Sent: Monday, April 09, 2001 9:15 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Preparation of a steel wedge on a ceramic substrate
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} Robin,
}
} The tripod polisher may just work. Although your sample is
} larger than
} anything I have previously tripoded, it should work if you use a plain
} L-bracket. First, planarize your sample and the two back feet of the
} tripod polisher. Then adjust the two back feet to give you
} the wedge angle
} you desire (just slightly over 1 degree if my trigonometry
} for your sample
} is correct). Likewise, the Multiprep system from Allied High
} Tech should
} be able to accept and wedge polish a sample of this size.
}
} If this approach does not work then you may need to use a
} precision lapping
} and polishing jig. You can either design your own or you may
} want to check
} with South Bay Technology. They make several jigs with
} precise angular
} control for polishing crystals. I don't know however, if they
} will work
} with samples this large.
}
} Hope this helps.
}
} Eric Windsor
}
} Disclaimer: I have no financial interest in either South Bay
} Technology or
} Allied High Tech Products. I am a satisfied customer of
} both. Also, there
} may be other products on the market that will work equally well for
} preparing this sample.
}
} The opinion expressed is my own and not that of my employer (NIST).
}
} Original Message:
}
} We have a professor here who has a 1 cm x 2.5cm steel layer
} about 100 um in
} thickness on a ceramic substrate. The metal layer was sputter
} deposited
} onto the ceramic substrate. The ceramic substrate extends
} past the metal
} layer. He needs to get a thickness gradient across the steel
} layer along
} the 2.5 cm length. He would like to have one end at about 50
} microns in
} thickness and the other at 2 microns with a gradually
} decreasing thickness
} gradient. Steps down would be ok although a smooth transition
} would be
} better. We have a laser profilometer to measure anything that
} we produce.
} Does anyone out there have any ideas how to do this? Would a tripod
} polisher work? I thought about electropolishing and masking
} off portions at
} a time but I worry about what will happen at the interface
} between the
} ceramic and the metal. Could we alter a dimpler?
}
}
} Thanks,
} Robin Griffin
} UAB
}
}
}


From daemon Mon Apr 9 17:09:42 2001



From: timothy.quinn-at-tufts.edu
Date: Mon, 09 Apr 2001 18:06:20 -0400
Subject: I need to unsubscribe tell me how

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to unsubscribe. Can you direct me.

Thanks Tim Quinn U of Kansas Museum of Natural History


From daemon Mon Apr 9 17:37:19 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Tue, 10 Apr 2001 08:38:10 +1000
Subject: Re: Substitutes for absolute ethanol?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How you can get the licence if you do not SELL the alcohol?
Licence is only for selling..

} We have recently run into some difficulty when needing to reorder 200
proof
} ethanol, which we use for dehydration and infiltration of samples
(primarily
} many types of paper) prior to embedding in Spurrs epoxy, and for cleaning
} samples (non paper) and lenses of light microscopes. The chemical company
} selling the ethanol is insisting that we must have a liquor license before
} they will ship to us.




From daemon Mon Apr 9 18:26:20 2001



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Tue, 10 Apr 2001 09:20:25 +1000
Subject: Re: Scanners: quantitative accuracy

Contents Retrieved from Microscopy Listserver Archives
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Does anybody have experience (good or bad) with Imaging Plate scanners on
TEMs?

PLease reply on or off-line, I will post a summary - preserving anonymity
if necessary!

thanks

Sally




Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218 or 6125 8525
http://www.anu.edu.au/EMU



From daemon Mon Apr 9 22:28:30 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 09 Apr 2001 20:28:30 -0700
Subject: Re: Substitutes for absolute ethanol?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your point is well taken, but in this environment, there is
no distinction between purchase, application or consumption.

I'm in California and have not run into this situation
as yet. But I would bet that the same myopic rules
would apply. I buy ethyl alcohol from Ted Pella in
Redding, CA. But I don't know the specific proof of the
alcohol (Cat.# 19207). It comes in 200mL bottles and
poses no problem when purchased in lots of six or fewer
bottles.

gg

At 03:38 PM 4/9/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Apr 9 22:35:49 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 09 Apr 2001 20:36:30 -0700
Subject: Re: Scanners: quantitative accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How do you define "quantitative digitization?" i.e., what
variables are you dealing with in this respect? What are
the "absolute terms?"

Anyone else have some ideas about this topic?

gg


At 10:10 AM 4/9/2001, you wrote:

} I have been listening to the thread on scanners. Has anyone done
} tests of how accurate they are in absolute terms for quantitative
} digitization?
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering &
} Center for Transportation Nanotechnology
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu
} http://www.numis.nwu.edu http://www.ctn.northwestern.edu



From daemon Tue Apr 10 01:12:30 2001



From: Andrew.Campbell3-at-defence.gov.au
Date: Tue, 10 Apr 2001 14:56:40 +1000
Subject: SEC: UNCLASSIFIED:-Microscope Ergonomics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I am a Laboratory Technician currently studying for a Diploma in Occupational
Health and Safety. My last study module is an Action Research project which I
would like to do on the development of ergonomic microscopes and although there
is a lot of information on the problems involved in microscopy and methods to
relieve them I was wondering if any research has been done into the
effectiveness of ergonomic design for improving microscopy diagnosis, and where
I could get any papers on the subject. Thanks. Andy.




From daemon Tue Apr 10 03:32:03 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 10 Apr 2001 18:25:42 +1000
Subject: Re: GMA recipe

Contents Retrieved from Microscopy Listserver Archives
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Dear Kristen,

Perhaps the reason there was no recipe for the GMA is that you can vary the
component ratios alot depending on the tissue you're embedding. There are
several reviews from the 60s and 70s on different GMA recipes, at least for
embedding plant material. One "standard" mix for plant material is 95% v/v
pure GMA, 5% v/v polyethylene glycol, 0.15-1.0% w/v benzoyl peroxide
(catalyst). With more catalyst, polymerisation is faster and blocks are
harder, but too much produces brittle, bubbly blocks. PEG can be 0-10%,
PEG 200 and 400 are commonly used.

Like other methacrylate resins, GMA can be heat polymerised but you need to
exclude oxygen. You can either seal the GMA blocks in capsules - gelatin
capsules for example - dent the cap to exclude as much air as possible, or
cover flat embedding moulds so that they are completely sealed - e.g. use a
plastic vial cap as flat mould with another on top to seal. If you have
access to a vacuum oven, you can polymerise open moulds under nitrogen.

Time to polymerise depends enormously on the composition of the resin, try
60C overnight for starters if using a fairly "standard" mixture.

good luck,
cheers,
Rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 10 03:57:39 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 10 Apr 2001 04:57:26 -0700 (PDT)
Subject: Re: Substitutes for absolute ethanol?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

Just a minor correction / update. VG Microtech recently changed name to
Thermo VG Scientific, address and contact details remain the same, except
for the surface science website:

Surface science products: www.vgscientific.com
Polaron range: www.polaron-range.com

The Polaron range continues to be represented in the US by Energy Beam
Sciences.

Best regards,

Mike Wombwell
Polaron Range Business Manager
Thermo VG Scientific
The Birches Industrial Estate
Imberhorne Lane
East Grinstead
West sussex RH19 1UB
UK
Tel: +44(0)1342310296 (direct line)
Tel: +44(0)1342327211 (Switchboard)
Fax: +44(0)1342315074
email: mike.wombwell-at-scientific.com
Website: http://www.polaron-range.com

E & OE


-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: 09 April 2001 22:04
To: Tracey M. Pepper
Cc: microscopy-at-sparc5.microscopy.com


Tracey
VG Microtech make Polaron integrated column-mounted cryo systems
including a new system
designed for high-resolution work with FEGSEMs
Emitech have an off-microscope cryo-prep and transfer system suitable
for LTSEM with a conventional tungsten filament or LaB6 SEM
BalTec make various cryo transfer and preparation systems which could
be used to transfer specimens from e.g. a freeze-etcher to SEM

see http://www.kaker.com/mvd/list.html for addresses and contact
numbers for these companies
Chris

----- Original Message -----
} From: "Tracey M. Pepper" {tpepper-at-iastate.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, April 09, 2001 6:52 PM


Richard:
Not true in the good old US of A! 200 proof EtOH is considered a controlled
substance, and purchase of even small quantities is regulated. In the
Histology and EM Labs here, we use a fairly large supply, so there is a
company license which allows purchase of a specific amount, and that means
if demands go up dramatically there is a real issue of obtaining adequate
supplies from time to time. I think that the issue may well have to do with
the quantities being purchased. If one buys the odd bottle now and then,
there is no hassle. But when usage passes a certain point, then licenses
are necessary. Keeps all of the bureaucrats in jobs.
As for the license, Teresa, it is mostly just paperwork. Requires you to
specify purpose for use, amounts that will be required, etc. If I remember
correctly (had to do this myself a _very_ long time ago--now an internal
supply room clerk does it), this is an annual process. And, like most labs,
since you have to account for every drop of reagent that enters and leaves
the lab for EPA, state EPA, OSHA, etc, you will likewise enter the EtOH into
that stream, thus showing that you aren't using it for more pleasurable
ends.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
NB--personal opinions and experiences only expressed above.
On Tue, 10 Apr 2001 08:38:10 +1000, Vr. Richard Bejsak-Colloredo-Mansfeld
wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| How you can get the licence if you do not SELL the alcohol?
| Licence is only for selling..
|
| } We have recently run into some difficulty when needing to reorder 200
| proof
| } ethanol, which we use for dehydration and infiltration of samples
| (primarily
| } many types of paper) prior to embedding in Spurrs epoxy, and for
cleaning
| } samples (non paper) and lenses of light microscopes. The chemical
company
| } selling the ethanol is insisting that we must have a liquor license
before
| } they will ship to us.
|
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue Apr 10 07:15:22 2001



From: Boucher, Germaine G :      germaine_g_boucher-at-groton.Pfizer.com
Date: Tue, 10 Apr 2001 08:11:52 -0400
Subject: Freeze dry protocols

Contents Retrieved from Microscopy Listserver Archives
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I am interested in freeze drying pieces of murine liver tissue for
subsequent embedment in Spurr's epoxy resin. Does anyone have experience
with this technique? Specifically I would be interested in times and
temperatures. I will be using a turbo freeze dryer from EMS to freeze dry
the tissue.

Thanks in advance,

Germaine G. Boucher
TEM Lab
Pfizer Global Research and Development
Groton, CT




From daemon Tue Apr 10 07:55:10 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 10 Apr 2001 07:50:22 -0500
Subject: RE: Scanners: quantitative accuracy

Contents Retrieved from Microscopy Listserver Archives
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Laurie and Gary,

Would noise be a good criterion? Say, for a perfectly evenly darkened film
(if such a thing existed, or at least even on a scale { { collected pixel
size) - what is the value of the noise (standard deviation of pixel value)
as a function of film darkness (density)?

This would presumably improve with the time of collection. Thus how "good"
your scanner is depends on how you run it or whether it lets you take a
slower scan or to average multiple scans. With the exception of drum
scanners these devices all use CCD arrays. So what is probably most of
interest is the signal to noise ratio as a function of illumination
intensity, with everything known about CCD's going into determining this.
The maximum density the scanner can handle is just the point at which the
noise swamps the signal.

There must be some good literature out there on the sources of noise,
optimizing collection (scan) time etc. One article which might be a
starting point is:

G. H. Campbell, W. E. King and D. Cohen "Analysis of Experimental Error in
High Resolution Electron Micrographs", Microscopy and Microanalysis vol. 3
(1997) p. 451.

This is not very detailed, and treats only the total random noise, i.e.
grouping noise arising in collecting the image with that arising from the
scanner.

Now, finding a good "Consumer Report" test with hard numbers on commercial
models is likely to be a lot harder!

Wharton

} -----Original Message-----
} From: Gary Gaugler [SMTP:gary-at-gaugler.com]
} Sent: Monday, April 09, 2001 10:37 PM
} To: L. D. Marks
} Cc: MSA listserver
} Subject: Re: Scanners: quantitative accuracy
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} How do you define "quantitative digitization?" i.e., what
} variables are you dealing with in this respect? What are
} the "absolute terms?"
}
} Anyone else have some ideas about this topic?
}
} gg
}
}
} At 10:10 AM 4/9/2001, you wrote:
}
} } I have been listening to the thread on scanners. Has anyone done
} } tests of how accurate they are in absolute terms for quantitative
} } digitization?
} }
} } -------------------------------------------------------
} } Laurence Marks
} } Department of Materials Science and Engineering &
} } Center for Transportation Nanotechnology
} } Northwestern University
} } Tel: (847) 491-3996 Fax: (847) 491-7820
} } mailto:ldm-at-risc4.numis.nwu.edu
} } http://www.numis.nwu.edu http://www.ctn.northwestern.edu
}


From daemon Tue Apr 10 09:09:30 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Tue, 10 Apr 2001 10:02:03 -0400
Subject: 200 PROF ETHANOL

Contents Retrieved from Microscopy Listserver Archives
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To the person having trouble getting 200 proof ethanol:

If you are embedding in Spurrs, you do not need ethanol or P.O.. Just
dehydrate in E M grade acetone and infiltrate with acetone/Spurrs and you
will get good results. Keep it simple, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Tue Apr 10 09:25:45 2001



From: jeanross :      jeanross-at-blue.weeg.uiowa.edu
Date: Tue, 10 Apr 2001 09:22:15 -0500
Subject: EDS summary

Contents Retrieved from Microscopy Listserver Archives
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I have put together a summary of responses I got from my inquiry about a week
or so ago about EDS systems. I really appreciate everyone's input. We
haven't made any decisions yet since we are still gathering information but
your responses will help. I've included the responses in their entirety so I
hope this helps others as well.

Thanks again from everyone who contributed.

Jean Ross
Central Microscopy Research Facility
University of Iowa

-----------------------------------------------------------------------------
I have been using an IXRF EDS / Gresham Detector (with IXRF digital pulse
processor) system for almost 2 years. Initially, the IXRF was installed on
an ETEC. About 15 months ago the Etec was replaced with a Hitachi 3500, a
new detector (Gresham) was purchased, and the EDS was installed on the 3500.
Generally, I am quite satisfied. There are minor software bugs, but IXRF
has been reasonably good at fixing them when discovered. It has been my
experience that all the systems have bugs, perhaps some more than others.
Prior to the IXRF, I had a Kevex 8000/Delta.

Low end noise and broad peaks were evident on first installation, but were
soon fixed by tweaking the detector preamp and pulse processor amp.

I am still running their first software package, "Iridium". I have the
newest release, "EDS 2000", but lack of time has kept me from installing and
checking it out.

I should mention that IXRF is a "virtual" company, with people spread out
between Texas, California, etc. This has not proven to be a problem.

Woody White
McDermott Technology, Inc.
nwwhite-at-mcdermott.com

-----------------------------------------------------------------------------


We have had EDAX for about a decade and a half, and we are very pleased with
the product and the service we get.
Carol Heckman
heckman-at-bgnet.bgsu.edu

-----------------------------------------------------------------------------


look at IXRF eds systems there web site is www.ixrfsystems.com, they are
very affordable and offer no nonsense performance that second to none.

happy ixrf user,

James Fotinopoulos
yzfrjim-at-ix.netcom.com


------------------------------------------------------------------------------

I would recommend you give consideration to Doug Connors at
TN Analyzer Service, Inc. of Dane, WI. Doug has rebuild and
upgraded detectors for me for the last 6 years. He is dependable
knowledgeable, and economical as well.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
(480) 967-3946
bobrobs-at-earthlink.net

-----------------------------------------------------------------------------


We recently purchased a Noran Instruments Vantage DS1. We purchased this
based on some very impressive demonstrations of software that the salesperson
brought in. Unfortunately they are still working out the bugs in their
software. Everything they have is ported over from Unix, and literally runs
in a unix shell on an Microsoft Windows NT platform. This makes their
software fairly buggy. Their response time to fix major bugs and hang-ups in
the software has been very slow, and if given the opportunity to do it all
over again I'd probably look at Oxford Instruments. I would still rate the
quality of the equipment very high. Our detector performs at the specified
resolution, and is a good piece of equipment. Now if they could only get the
software end of it straight...
My vote:
1) Oxford Instruments
2) Noran Instruments
3) Edax or some of the smaller players
The benefit with going with a larger company is support and upgrades. We have
a 10 year old WDX that we just purchased new software and interface for last
year. Our old EDS was given some trade in value by Noran. And we all know
how valuable a service contract can be...

Get back to me if you have any more questions,
~Jonathan
Jonathan Dunlap
Analytical Laboratory Manager
Osram Sylvania Inc.
816 Lexington Avenue
Warren, PA 16365
Ph: 814-726-6991
Fax: 814-726-6942
Jonathan.Dunlap-at-sylvania.com


------------------------------------------------------------------------------


We have an Oxford Instruments Link ISIS Model 200 on our 2460N. We have been
happy with it, but I don't know about the direction that Oxford is heading. I
don't care for the feel of their new INCA software. Some might like it. It
also seems to be slow coming together. Some of the functions are still lacking
after 2 (or is it 3) years of seeing it at MSA.

We have an IXRF system on our JEOL 840A. It was a good price ($30K) for an
upgrade to our Kevex several years ago. It does what we need. They keep at
work on the software and have it freely available on the web. I might have to
pay closer attention and stay away from the beta stuff. They are still working
on it. They also have a nice digital pulse processor which stills stand alone
for about $5k.

I still feel funny about some contacts with EVEX. I can't say much about EDAX,
NORAN, or PGT. They should all have good stuff but it might be pricey. The
last we seriously looked at them was 6 years ago or so when we opted for the
Oxford.

I was intrigued by the unit from Quartz PCI. I think it was called X-ray One,
or such. It was new at MSA 1-1/2 years ago but looked promising.

Feel free to call if you want more details.

Warren E Straszheim
wesaia-at-iastate.edu

-----------------------------------------------------------------------------


We purchased an EDAX Falcon system for our Hitachi S-3000N and I've been
pleased with it. It has better light element sensitivity than most which
was very important to me although I don't think that its mapping
capabilities are as good as PGT's, say. I don't have direct experience with
Noran although I did talk to them and their system seemed ok - but logistics
didn't favor Noran so I passed on them. EDAX does have good integration
with the Hitachi and the Quartz database.

I'd be glad to respond more specifically if you'd like.

Richard Shalvoy
Arch Chemicals
Cheshire, CT
RBShalvoy-at-archchemicals.com

-----------------------------------------------------------------------------


I have an iXRF systems out of Texas using a Gresham detector. It works
well. Not the most cutting edge, but they are one of the "start ups". They
have been around for I guess 6-7 years. I have a digital pulse processor
and completely active control for x-ray maps and such. They are very price
competitive, but lack a dedicated technical support person. You talk to the
programmer or electronics expert, but no techs on the phone whenever you
have a software question. But, if you willing to wait a day for some
answers then they are worth it. I haven't run across the problem where I
thought, "if I just had a better system". If you want to integrated w/ WDS
than maybe Noran. Also, if you want to integrated w/ motorized stage
control, I don't think they off such a package, like the bigger companies.

I have a Hitachi 450. I used to run a 2400 and 500 before I quit my day job
and went out on my own. I am very happy w/ Hitachi.

Good Luck

Fell free to call with any specifics.

Their web page is www.ixrfsystems.com

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756
smartech-at-javanet.com

------------------------------------------------------------------------------


Hello, all:

I use Oxford ISIS300 system on HITACHI S-3500N (with VP mode) for light
element analysis, mostly C, O, N, F, P, S, Si, Mg, as well as metal Co, Ni-P,
Pt, Cr, Fe, W, etc. This system works well. One useful function is the overlay
of 2 spectrums. I can easily subtract the blank from the sample spot and make
it easy to identify what is (are) in the sample. I am sure some other program
may have this kind of function, but I have not seen.

Zhiyu Wang
zhiyuw-at-home.com
I would be interested in seeing the responses as I am going to try and get
funding next year for a replacement for our EDAX PV9100 on an Hitachi s-450.

Dave
David.Patton-at-uwe.ac.uk

-----------------------------------------------------------------------------


We have been running EDX on SEMs and TEMs for many years. We used to have a
range of systems from Kevex, PGT, Noran, Link, EDAX, however a few years ago
we decided that we ought to standardize on one common system. After evaluation
we bought three Oxford Instruments ISIS systems. Whenever we have upgraded or
bought new systems they have been Oxford Instruments ISIS or now INCA.

I have been happy with the ISIS except for the file handling that was not
designed for a multi user facility such as ours (approx 120 EM users in total
roughly 25 to 30 swapping every year). I am really quite impressed by the
INCA, Oxford Instruments are, at last, listening to the users and adding user
requested facilities. They have sorted out the file handling mess of the ISIS
and structured it well for an SEM user (not quite as well for a TEM user but
there are less of us). The software structure is quite intuitive and there is
a really impressive help menu and explanation of everything from the physics
of X-ray generation, how EM’s work, how detectors work and how to analyze
samples.

Their detectors have always been good and the SATW (thin window detectors)
still have a reasonable efficiency at low Z. B is possible but C is easy and
even the N peak is over 30% efficient (there is often a high absorption at N).

Another feature that is invaluable for TEM is the integral shutter that will
close when the count rate is too high. This protects the crystal, it prevents
it overloading and shutting down or worse the crystal efficiency may change
for a few minutes until it recovers fully. This may affects your quantitative
work. In TEM this is usually caused by hitting the grid bar and not really a
problem in SEM but I don't know what secondaries and ions you will have in a
variable pressure SEM. It could be useful for you, check with other high
pressure SEM users.

Regards,
Ron

Please note: Oxford Instruments have upgraded an ISIS to an INCA system in my
department, without charge, in return for access to the instrument for
development projects and demonstrations for a fixed number of days. I receive
no benefit from this and the department has no benefit from Oxford Instruments
sales. I remain a thorn in the flesh of all our suppliers if I think they
could improve their products or service.
ron.doole-at-materials.oxford.ac.uk


------------------------------------------------------------------------------


Hello,
I am very familiar with the Oxford ISIS 300 series spectrometers. They are
ok, and the new Inca system looks good too. However, I recently saw the PGT
spectrometer at Lehigh and it is very impressive.

Steve
Stephen_Skirius-at-bkitech.com



--------------------------------------------------------------------------


I'm also in the market for an EDS system and have looked at EDAX, Noran,
PGT and Oxford.

I edited out PGT because in order to quantify you have to optimize the
system for the type of sample by playing with fudge factors, which none of
the other systems have to do (though one of them, I think Noran, lets you
adjust a sensitivity factor if you want to, but they didn't do it on my
samples that were tested against known microprobe results and the answers
were fine). I also eliminated Oxford, though it has a terrific user
interface (maybe at the expense of functionality), because they
consistently IDed my aluminum peaks as Br or Tm (!); this made me wonder
about all their algorithms. They claim it had to do with the takeoff angle
on the particular SEM being used, but that shouldn't be a factor.

I like both EDAX and Noran, though for different reasons. EDAX user
interface is better than Noran's, though again, I think Noran possibly
offers more routines (it's hard to keep track and see absolutely everything
a system has to offer in a demo day....).Noran can multitask - work on
several programs while a spectral map is being collected, for instance
(does EDAX? I have to check). But EDAX has a beam skirt reduction routine
for low vac mode (though it's time consuming, so a bit cumbersome), and
their peak modeling is right up front - but Noran can put theirs up front
also if you want to have it accessible (yes) and I think Noran might be a
little better engineered.

As you can see I'm still in a quandary (ditto for the two contending SEMs,
LEO and ESEM). Whatever I decide I'll still be very interested in the
results of your posting - especially if other folks' info comes in within
the next week or so it would help in my decision too.

I hope my input helps a little. Good luck with your quest!

Dee Breger
micro-at-ldeo.columbia.edu


---------------------------------------------------------------------------


I looked into Noran, EDAX, and PGT. Noran was quickly culled (less user
friendly, less abilities, didn't work right during demo), but EDAX and PGT
both seemed to have equivalent capabilities (PGT claimed a 'proprietary'
signal amplifier/digitizer doohickey, but it was only in the placement.)
For the long-time spectrum gathering (I forget the technical term), PGT
makes many passes with short dwell times while EDAX dwells on each pixel
much longer to collect data & does it in 1 pass. Kinda 6 of one, half dozen
of the other. What made us choose the EDAX Phoenix system was the fact that
the PGT software was UNIX-based (although hidden) while EDAX is PC-based.
I've heard rumors that PGT is switching to PC-based; we purchased our system
in 1999. I've also heard that Noran has practically no service techs (but
that may be an East coast thing.) We've been happy with the EDAX service,
and I enjoyed their user school very much. By the way, our SEM is a
variable pressure JEOL 5900, and it's integrated with the EDAX system.

Hope I've been helpful,

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
jlagoy-at-bodycote-imt.com




From daemon Tue Apr 10 09:51:53 2001



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Tue, 10 Apr 2001 09:58:52 -0500
Subject: TEM-SiC wafer sample prep?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
I have been asked to examine some films on a Si carbide substrate using
TEM. Does anyone out there work with this material. Can one
mechanically thin it using diamond films? Please offer some suggestions
of what you are doing.
Thanks in advance,
Michael Coviello
University of Texas Arlington




From daemon Tue Apr 10 11:31:37 2001



From: R. Howard Berg :      rhberg-at-danforthcenter.org
Date: Tue, 10 Apr 2001 11:26:36 -0500
Subject: dye transfer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have some staining that suggests the staining dye has transferred from
one place to another and I write to see if anyone could shed some light on
this for us.

In the experiment we stain isolated chromosomes with DAPI, rinse them, and
then introduce them into plant protoplast cells (likely one or few per
cell). There is no autofluroescence in the DAPI channel and we can follow
the course of the dyed chromosomes over time. At first the DAPI
fluorescence appears in the cytosol on structures likely to be the
introduced chromosomes. Then it appears in the nucleus, where all the
chromosomes are stained! This is especially evident when we culture these
cells. Dividing cells at metaphase show DAPI fluorescence over the entire
metaphase plate. Note that only some cells show DAPI fluorescence,
consistent with the presumption that our fusion process that introduces the
chromosomes is only successful in some of the cells.

Has anyone had an experience where dye has been transferred from one
structure to another in a living cell? What are some alternative
interpretations of this phenomenon?

Thanks for your comments,

Howard Berg


R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility,
Associate Member
Donald Danforth Plant Science Center/Nidus Center
893 North Warson
St. Louis, MO 63141

phone: 314-812-8076
fax: 314-812-8127
cell phone: 314-378-2409

http://www.danforthcenter.org





From daemon Tue Apr 10 11:57:10 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Tue, 10 Apr 2001 13:24:23 -0400
Subject: Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Mike,

We tripod polished thin films on SiC. It works OK with diamond lapping
film and diamond powder slurry as a final polish, but it takes a loooonnng
time. Put a sacrificial piece of SiC on the second side tool pedestal so
the final wedge polish will go from SiC sample to SiC tool. Otherwise the
glass pedestal polishes faster than the SiC and makes a little step that
can lead to sample breakage. ...Always a good idea to match the pedestal
hardness to the specimen--hard or soft.

As I recall, we had more problems with poor film adhesion to the SiC than
with polishing the SiC substrate.

Although we haven't tried it, I suppose a FIB would work fine.

Ron




There was a thread recently on scanners for TEM film. I have looked up
all the models mentioned, on the web and called agents for prices - and
produced a comparative table, given below.

I do not guarantee that the figures are accurate but they are my best
interpretation of the data given.

In the light of experience and Nestor's comments, I would suggest that
2000 dpi is a minimum for TEM negatives. You may be able to get away
with less nine times out of ten, but there will be occasions when you
need more.
I would exclude the Minolta and all the Epsons from consideration
(despite the incredibly low prices of some of the Epsons) because of the
low pixel density.

Among the rest the Nikon has the best pixel density and the best optical
density (another critical parameter for TEM negatives). The price is
very competitive too. The Nikon web site does not give a time for
scanning a negative. On the face of it the Nikon would be a best buy -
get a separate, inexpensive flatbed scanner for the other work.

These comments are all my own opinions based on manufacturers' data.
Since we are considering purchase any comments to the contrary would be
most welcome.



Code Maker Model Type



A Agfa DuoScan T2500 Flatbed -Transparency included

B Epson 1640 several versions Flatbed -Transparency option
1680 several versions

C 1600 several versions Flatbed -Transparency included

D Imacon Flextight Precision II Drum -for film and large format

E Minolta Dimage ScanMulti II Film

F Nikon Super Coolscan 8000ED Film

G Polaroid 45 Ultra Film

H Umax Powerlook 3000 Flatbed -Transparency included





Code dpi OD Time Price Opinion
at 6 x 9 cm


A 2500 x2500 3.4 3 min $4,500 Fair

B 1600 x 3200 3.6 $300-$3000 Poor
$800-$1400 Poor

C 1600 x 3200 3.3 $650-$1160 Not suitable

D 2240 x2240** 3.9/4.1 N/A above $10k Good: low pixel density

E 1128 x 1128 3.6 Not suitable

F 4000 x 4000 4.2 N/A $2,695 V. Good

G 2500 x 2500 3.8 5 min $7,495 Good but pricey

H 3048 x 3048 3.6 3 min $6,499 Good


--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Tue Apr 10 13:32:25 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 10 Apr 2001 13:22:23 -0500
Subject: Re: Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I too am about to buy and I would make a couple of comments on your
evaluation. First, let me remind everyone that the Dynamic range is
a log scale so small numerical differences are significant.

I also think the Nikon Coolscan 8000 looks great but it only takes a
2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers gone
to a smaller film size or is Nikon using a non-Japanese EM as their
standard? seems odd but I don't see how the Nikon would be very
useful. You say a {2000 line scanner would be useful 9 out of 10
times but want the 2000+ lines for the occasional high res scan. I
would argue that the size of the negative was the more important
variable to be worried about. The Nikon couldn't handle 4x5 LM
negatives or transparencies from autoradiography of
Westerns/Northerns, etc.

My leading candidate is the ArtixScan 1100 has a Dmax of 3.9 (about
$1600 with SCSI card). This was has a 1000 x 2000 dpi resolution.
more details at www.microtek.com. This is my leading candidate. It
was 4 negative carriers and I await word whether one could be
modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will have
my scientific instrumentation shop guys fabricate a holder. It comes
with a glass 8 x 10 glass carrier for odd size negs but I want to
avoid Newton rings and want a glassless carrier.

I would appreciate comments on the following argument (I think I have
this correctly figured out but am not sure since so many out there
seem to want to have a higher resolution scanner). I have a Fuji
Pictrography 3000 printer with a 400 dpi output that is as good as
any other widely available printer in the academic world. If you
figure the maximum published image size is about 8 inches, that would
mean the maximum image size be 3200 dpi wide. A 1000 dpi scan of my
negative would be 4500 x 3500 dpi. I could crop by about 28% or 10%
depending on the orientation of the negative and still be taking full
advantage of the printer resolution. In reality, most EM publication
prints are smaller than 8" wide so one could crop even more and still
not need more than 1000 dpi. A resolution } 1000 dpi would be
useful for subtle morphometric analysis but a 4000 dpi scan of a 3 x
4 negative would be 192 MB. That is pretty big for doing morphometry
on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16 MB
and that is much more manageable. Perhaps the difference is in the
type of EM we are doing. I am working with biological specimens
doing standard thin section type stuff. are you doing some Material
Sci application that demands more?


I will be interested in Alwyn (and any others) reply since I hope to
buy one soon!


} .
}
}
} There was a thread recently on scanners for TEM film. I have looked up
} all the models mentioned, on the web and called agents for prices - and
} produced a comparative table, given below.
}
} I do not guarantee that the figures are accurate but they are my best
} interpretation of the data given.
}
} In the light of experience and Nestor's comments, I would suggest that
} 2000 dpi is a minimum for TEM negatives. You may be able to get away
} with less nine times out of ten, but there will be occasions when you
} need more.
} I would exclude the Minolta and all the Epsons from consideration
} (despite the incredibly low prices of some of the Epsons) because of the
} low pixel density.
}
} Among the rest the Nikon has the best pixel density and the best optical
} density (another critical parameter for TEM negatives). The price is
} very competitive too. The Nikon web site does not give a time for
} scanning a negative. On the face of it the Nikon would be a best buy -
} get a separate, inexpensive flatbed scanner for the other work.
}
} These comments are all my own opinions based on manufacturers' data.
} Since we are considering purchase any comments to the contrary would be
} most welcome.
}
}
}
} Code Maker Model Type
}
}
}
} A Agfa DuoScan T2500 Flatbed
} -Transparency included
}
} B Epson 1640 several versions Flatbed
} -Transparency option
} 1680 several versions
}
} C 1600 several versions Flatbed
} -Transparency included
}
} D Imacon Flextight Precision II Drum -for
} film and large format
}
} E Minolta Dimage ScanMulti II Film
}
} F Nikon Super Coolscan 8000ED Film
}
} G Polaroid 45 Ultra Film
}
} H Umax Powerlook 3000 Flatbed
} -Transparency included
}
}
}
}
}
} Code dpi OD Time Price
} Opinion
} at 6 x 9 cm
}
}
} A 2500 x2500 3.4 3 min
} $4,500 Fair
}
} B 1600 x 3200 3.6
} $300-$3000 Poor
}
} $800-$1400 Poor
}
} C 1600 x 3200 3.3
} $650-$1160 Not suitable
}
} D 2240 x2240** 3.9/4.1 N/A above
} $10k Good: low pixel density
}
} E 1128 x 1128 3.6
} Not suitable
}
} F 4000 x 4000 4.2 N/A
} $2,695 V. Good
}
} G 2500 x 2500 3.8 5 min
} $7,495 Good but pricey
}
} H 3048 x 3048 3.6 3 min
} $6,499 Good
}
}
} --
} ..........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Apr 10 14:02:54 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 10 Apr 2001 14:58:55 -0400
Subject: RE: TEM-SiC wafer sample prep?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I assume that your SiC is single crystal. The small angle cleavage technique works for SiC. See MRS Proceedings, TEM Prep IV Vol 480.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Michael Coviello [mailto:coviello-at-mae.uta.edu]
} Sent: Tuesday, April 10, 2001 10:59 AM
} To: listserver
} Subject: TEM-SiC wafer sample prep?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi All:
} I have been asked to examine some films on a Si carbide
} substrate using
} TEM. Does anyone out there work with this material. Can one
} mechanically thin it using diamond films? Please offer some
} suggestions
} of what you are doing.
} Thanks in advance,
} Michael Coviello
} University of Texas Arlington
}
}
}


From daemon Tue Apr 10 14:28:31 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Tue, 10 Apr 2001 14:17:42 -0500
Subject: Sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:

I want to buy a new/used sputter coater which enables to do rotary
shadowing as well as carbon coating. Any suggestions/input are
appreciated.

Greg Ning

EM Facility
Medical College of Wisconsin



From daemon Tue Apr 10 14:30:04 2001



From: Francis W. Flynn :      Flynn-at-uwyo.edu
Date: Tue, 10 Apr 2001 13:25:33 -0600
Subject: listserve job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Scientist for two Recently Established NIH Centers for
Biomedical Research at The University of Wyoming

As part of our seven interrelated projects into the biology, chemistry, and
molecular biology of cardiovascular function and nitric oxide we seek a
skilled microscopist to be hired at the non-tenured Assistant Research
Professor level. Experience and research interests in state-of-the-art
microscopy, including confocal and epifluorescence, and ultrastructural
techniques. Primary responsibility will be management of the University's
Microscopy Center. Opportunity includes the possibility of establishing a
research program within this Center. Appointment will be at a (non-tenure
track) Assistant Professor level for a renewable four year term.

Details:
Start_Search_Date: April 5, 2001
End_Search_Date: N/A
Job_Title: Confocal Microscopy/Electron Microscopy Technologist

Job Description: The person we seek will be responsible for organization of
a new research laboratory facility at the University of Wyoming which will
include two confocal microscopes and an electron microscope. The position
includes overall management of the microscope facility, user training, and
user supervision. Requirements for the position include experience with
light, confocal, and transmission electron microscopy. This individual will
oversee all aspects of specimen accession and processing, operation of the
microscopes, photography, and record keeping. The individual will work with
only minimum supervision. Responsibilities include: Serve as the technical
manager of the facility and be responsible for the operation and maintenance
of the confocal and EM microscope facility. In addition the manager will
perform preventative maintenance on the equipment; maintain the lab, order
supplies, schedule instruments, and oversee billing. Image analysis at the
light, confocal and electron microscopic levels and preparation of
micrographs for publication.
Applicant Qualifications: Experience focus on both confocal and EM.
Regarding confocal microscopy, we require experience with confocal and
digital imaging techniques, visualization of living cells containing
fluorescent probes, photobleaching, and fluorescence in situ hybridization.
The successful applicant will have experience with tissue preparation for EM
and the maintenance of an electron microscope. Excellent interpersonal and
organizational skills are essential. Ph.D.. degree required
Desirable Experience: Expertise and training in the operation of confocal
microscope and EM microscope systems is required. Familiarity with light
microscopy methods, immunofluorescent staining, use of fluorescent probes
for physiologic measurements and the general principles of cell biological
research are desirable. Significant facility with computers is desired.
Salary Range: Commensurate with experience.

For additional information see our websites:
www.uwyo.edu/nocobre
www.uwyo.edu/MolecBio/Cobre
To apply send complete CV, three references, and a cover letter indicating
which position(s) you are applying for to: Lynda Payne, Department of
Chemistry, University of Wyoming, Laramie, WY 82071-3838, USA. The searches
will remain open until all positions are filled.

The University of Wyoming is located in a high (2,200 m) valley surrounded
by the Rocky Mountains in the southeast corner of Wyoming. The University of
Wyoming is an equal opportunity/affirmative action employer.



From daemon Tue Apr 10 15:25:17 2001



From: jshields-at-cb.uga.edu
Date: Tue, 10 Apr 2001 16:19:55 -0400
Subject: Southeastern microscopy newsletter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Beam, newsletter for the Southeastern Microscopy Society
(SEMS) is now online at the SEMS website in PDF format:
http://www.biotech.ufl.edu/sems/

It contains information on the upcoming meeting in Clemson, SC.
If you are a member, and have not received this notice via e-mail,
and wish to be informed about the society through e-mail, please
respond off-listserve at:
jshields-at-cb.uga.edu

John Shields
Center for Ultrastructural Research
Univ. of Georgia
Athens, GA


From daemon Tue Apr 10 15:41:33 2001



From: Hayes, Fred :      FHayes-at-TAC.Textron.com
Date: Tue, 10 Apr 2001 16:38:00 -0400
Subject: Ultracut E

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking to buy a used Ultracut E with FC4E cryo unit, in good working order

contact

Fred Hayes
FHayes-at-TAC.Textron.com


From daemon Tue Apr 10 16:05:42 2001



From: Joseph C. Besharse :      jbeshars-at-mcw.edu
Date: Tue, 10 Apr 2001 16:12:21 +0000
Subject: Re: Sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg:

Such an instrument could provide for low-angle rotary shadowing capability
for visualizing purified proteins at high resolution. It would need to
have an electron beam gun for platinum evaporation and a separate one for
carbon coating.

If this is what you have in mind, I support it. I purchased such an
instrument that had good performance for about $20,000 about 10 years ago.

Dr. Joseph C. Besharse
Professor and Chairman
Dept of Cell Biology,
Neurobiology and Anatomy
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, WI 53226-0509

Phone: 414-456-8261
Fax: 414-456-6517
E-mail: jbeshars-at-mcw.edu
Website: http://www.mcw.edu/cellbio/

} From: Gang Ning {gning-at-mcw.edu}
} Organization: Medical College of Wisconsin
} Reply-To: gning-at-mcw.edu
} Date: Tue, 10 Apr 2001 14:17:42 -0500
} To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
} Cc: "Dr. Traktman" {ptrakt-at-post.its.mcw.edu} , Ming Lei {mlei-at-mcw.edu} , "Dr.
} Besharse" {jbeshars-at-mcw.edu}
} Subject: Sputter coater
}
} Hi All:
}
} I want to buy a new/used sputter coater which enables to do rotary
} shadowing as well as carbon coating. Any suggestions/input are
} appreciated.
}
} Greg Ning
}
} EM Facility
} Medical College of Wisconsin
}



From daemon Tue Apr 10 16:44:57 2001



From: s2007282-at-student.rmit.edu.au
Date: Tue, 10 Apr 2001 16:42:59 -0500
Subject: Ask-A-Microscopist: microscope kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: s2007282-at-student.rmit.edu.au
Name: Kade

Organization: RMIT Melbourne Australia

Education: Graduate College

Location: Melbourne, Victoria, Australia

Question: Hi, I have just purchased a microscope, a good biological
one. I need a microscope kit but nobody sells them around here.
Anyway already I have glass slides and covers. I have read some on
microscopy and I need an adhesive, resin I think its called to
prepare slides? is this true?
Also some ink to stain specimens. What are the names of all these
chemicals so I can buy them all seperatly since no one sells them all
together.
Thankyou.

---------------------------------------------------------------------------


From daemon Tue Apr 10 16:53:27 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Tue, 10 Apr 2001 16:52:30 -0500
Subject: RE: Substitutes for absolute ethanol?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Theresa:
Although a metallographic laboratory, we use alcohol for
specimen preparation, mainly for preparation of etchants and for specimen
cleaning. We found the paperwork associated with pure ethanol onerous and
switched to denatured alcohol Type 3A with no discernable difference. Beware
of some of the denaturants, they produce unusual side effects.

Sam Purdy
National Steel Tech Center
Trenton MI



} ----------
} From: BOES,TERESA (HP-Corvallis,ex1)
} Sent: Monday, April 9, 2001 2:50 PM
} To: 'microscopy-at-MSA.microscopy.com'
} Subject: Substitutes for absolute ethanol?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone used any of the denatured ethanols as a substitute for absolute
} ethanol?
}
} We have recently run into some difficulty when needing to reorder 200
} proof
} ethanol, which we use for dehydration and infiltration of samples
} (primarily
} many types of paper) prior to embedding in Spurrs epoxy, and for cleaning
} samples (non paper) and lenses of light microscopes. The chemical company
} selling the ethanol is insisting that we must have a liquor license before
} they will ship to us.
}
} Ethanol denatured with a variety of substances is readily available and
} can
} be shipped with no licensing requirements. Our concern is that the
} denaturing agent will leave a detectable residue on lenses, samples, and
} may
} cause problems with the polymerization of Spurrs. Rather than obtaining a
} liquor license, we are considering using one of the 100:5 ethanol:
} methanol
} blends. If any of you have had successful or unsuccessful experiences
} substituting denatured ethanol for absolute in embedding or cleaning
} protocols, I would appreciate hearing from you.
}
} Teresa Boes
} Hewlett-Packard
} Analytical and Development Lab
} 1000 Circle Blvd
} Corvallis, OR 97330
} 541-715-7055
} teresa_boes-at-hp.com
}
}


From daemon Tue Apr 10 17:14:30 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 11 Apr 2001 08:55:11 +1000
Subject: RE: Sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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Jean,

I would caution biased opinions from installation engineer and sales
representatives ie... James Fotinopoulos.... www.semguy.com...

Hmmmmmmmmm?

Food for thought





----- Original Message -----

} From: "jeanross" {jeanross-at-blue.weeg.uiowa.edu}
} To: "Microscopy Listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, April 10, 2001 10:22 AM
} Subject: EDS summary
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have put together a summary of responses I got from my inquiry about a
} week
} } or so ago about EDS systems. I really appreciate everyone's input. We
} } haven't made any decisions yet since we are still gathering information
} but
} } your responses will help. I've included the responses in their entirety
} so I
} } hope this helps others as well.
} }
} } Thanks again from everyone who contributed.
} }
} } Jean Ross
} } Central Microscopy Research Facility
} } University of Iowa
} }
} } --------------------------------------------------------------------------
} ---
} } I have been using an IXRF EDS / Gresham Detector (with IXRF digital pulse
} } processor) system for almost 2 years. Initially, the IXRF was installed on
} } an ETEC. About 15 months ago the Etec was replaced with a Hitachi 3500, a
} } new detector (Gresham) was purchased, and the EDS was installed on the
} 3500.
} } Generally, I am quite satisfied. There are minor software bugs, but IXRF
} } has been reasonably good at fixing them when discovered. It has been my
} } experience that all the systems have bugs, perhaps some more than others.
} } Prior to the IXRF, I had a Kevex 8000/Delta.
} }
} } Low end noise and broad peaks were evident on first installation, but were
} } soon fixed by tweaking the detector preamp and pulse processor amp.
} }
} } I am still running their first software package, "Iridium". I have the
} } newest release, "EDS 2000", but lack of time has kept me from installing
} and
} } checking it out.
} }
} } I should mention that IXRF is a "virtual" company, with people spread out
} } between Texas, California, etc. This has not proven to be a problem.
} }
} } Woody White
} } McDermott Technology, Inc.
} } nwwhite-at-mcdermott.com
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } We have had EDAX for about a decade and a half, and we are very pleased
} with
} } the product and the service we get.
} } Carol Heckman
} } heckman-at-bgnet.bgsu.edu
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } look at IXRF eds systems there web site is www.ixrfsystems.com, they are
} } very affordable and offer no nonsense performance that second to none.
} }
} } happy ixrf user,
} }
} } James Fotinopoulos
} } yzfrjim-at-ix.netcom.com
} }
} }
} } --------------------------------------------------------------------------
} ----
} }
} } I would recommend you give consideration to Doug Connors at
} } TN Analyzer Service, Inc. of Dane, WI. Doug has rebuild and
} } upgraded detectors for me for the last 6 years. He is dependable
} } knowledgeable, and economical as well.
} }
} } Bob Roberts
} } EM Lab Services, Inc.
} } 2409 S. Rural Rd Suite C
} } Tempe, Arizona 85282
} } (480) 967-3946
} } bobrobs-at-earthlink.net
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } We recently purchased a Noran Instruments Vantage DS1. We purchased this
} } based on some very impressive demonstrations of software that the
} salesperson
} } brought in. Unfortunately they are still working out the bugs in their
} } software. Everything they have is ported over from Unix, and literally
} runs
} } in a unix shell on an Microsoft Windows NT platform. This makes their
} } software fairly buggy. Their response time to fix major bugs and hang-ups
} in
} } the software has been very slow, and if given the opportunity to do it all
} } over again I'd probably look at Oxford Instruments. I would still rate
} the
} } quality of the equipment very high. Our detector performs at the
} specified
} } resolution, and is a good piece of equipment. Now if they could only get
} the
} } software end of it straight...
} } My vote:
} } 1) Oxford Instruments
} } 2) Noran Instruments
} } 3) Edax or some of the smaller players
} } The benefit with going with a larger company is support and upgrades. We
} have
} } a 10 year old WDX that we just purchased new software and interface for
} last
} } year. Our old EDS was given some trade in value by Noran. And we all
} know
} } how valuable a service contract can be...
} }
} } Get back to me if you have any more questions,
} } ~Jonathan
} } Jonathan Dunlap
} } Analytical Laboratory Manager
} } Osram Sylvania Inc.
} } 816 Lexington Avenue
} } Warren, PA 16365
} } Ph: 814-726-6991
} } Fax: 814-726-6942
} } Jonathan.Dunlap-at-sylvania.com
} }
} }
} } --------------------------------------------------------------------------
} ----
} }
} }
} } We have an Oxford Instruments Link ISIS Model 200 on our 2460N. We have
} been
} } happy with it, but I don't know about the direction that Oxford is
} heading. I
} } don't care for the feel of their new INCA software. Some might like it. It
} } also seems to be slow coming together. Some of the functions are still
} lacking
} } after 2 (or is it 3) years of seeing it at MSA.
} }
} } We have an IXRF system on our JEOL 840A. It was a good price ($30K) for an
} } upgrade to our Kevex several years ago. It does what we need. They keep at
} } work on the software and have it freely available on the web. I might have
} to
} } pay closer attention and stay away from the beta stuff. They are still
} working
} } on it. They also have a nice digital pulse processor which stills stand
} alone
} } for about $5k.
} }
} } I still feel funny about some contacts with EVEX. I can't say much about
} EDAX,
} } NORAN, or PGT. They should all have good stuff but it might be pricey. The
} } last we seriously looked at them was 6 years ago or so when we opted for
} the
} } Oxford.
} }
} } I was intrigued by the unit from Quartz PCI. I think it was called X-ray
} One,
} } or such. It was new at MSA 1-1/2 years ago but looked promising.
} }
} } Feel free to call if you want more details.
} }
} } Warren E Straszheim
} } wesaia-at-iastate.edu
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } We purchased an EDAX Falcon system for our Hitachi S-3000N and I've been
} } pleased with it. It has better light element sensitivity than most which
} } was very important to me although I don't think that its mapping
} } capabilities are as good as PGT's, say. I don't have direct experience
} with
} } Noran although I did talk to them and their system seemed ok - but
} logistics
} } didn't favor Noran so I passed on them. EDAX does have good integration
} } with the Hitachi and the Quartz database.
} }
} } I'd be glad to respond more specifically if you'd like.
} }
} } Richard Shalvoy
} } Arch Chemicals
} } Cheshire, CT
} } RBShalvoy-at-archchemicals.com
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } I have an iXRF systems out of Texas using a Gresham detector. It works
} } well. Not the most cutting edge, but they are one of the "start ups". They
} } have been around for I guess 6-7 years. I have a digital pulse processor
} } and completely active control for x-ray maps and such. They are very price
} } competitive, but lack a dedicated technical support person. You talk to
} the
} } programmer or electronics expert, but no techs on the phone whenever you
} } have a software question. But, if you willing to wait a day for some
} } answers then they are worth it. I haven't run across the problem where I
} } thought, "if I just had a better system". If you want to integrated w/ WDS
} } than maybe Noran. Also, if you want to integrated w/ motorized stage
} } control, I don't think they off such a package, like the bigger companies.
} }
} } I have a Hitachi 450. I used to run a 2400 and 500 before I quit my day
} job
} } and went out on my own. I am very happy w/ Hitachi.
} }
} } Good Luck
} }
} } Fell free to call with any specifics.
} }
} } Their web page is www.ixrfsystems.com
} }
} } Ric
} }
} } SMARTech
} } 860-491-3299
} } www.semguy.com
} } 19 Cornwall Drive
} } Goshen CT 06756
} } smartech-at-javanet.com
} }
} } --------------------------------------------------------------------------
} ----
} }
} }
} } Hello, all:
} }
} } I use Oxford ISIS300 system on HITACHI S-3500N (with VP mode) for light
} } element analysis, mostly C, O, N, F, P, S, Si, Mg, as well as metal Co,
} Ni-P,
} } Pt, Cr, Fe, W, etc. This system works well. One useful function is the
} overlay
} } of 2 spectrums. I can easily subtract the blank from the sample spot and
} make
} } it easy to identify what is (are) in the sample. I am sure some other
} program
} } may have this kind of function, but I have not seen.
} }
} } Zhiyu Wang
} } zhiyuw-at-home.com
} } I would be interested in seeing the responses as I am going to try and get
} } funding next year for a replacement for our EDAX PV9100 on an Hitachi
} s-450.
} }
} } Dave
} } David.Patton-at-uwe.ac.uk
} }
} } --------------------------------------------------------------------------
} ---
} }
} }
} } We have been running EDX on SEMs and TEMs for many years. We used to have
} a
} } range of systems from Kevex, PGT, Noran, Link, EDAX, however a few years
} ago
} } we decided that we ought to standardize on one common system. After
} evaluation
} } we bought three Oxford Instruments ISIS systems. Whenever we have upgraded
} or
} } bought new systems they have been Oxford Instruments ISIS or now INCA.
} }
} } I have been happy with the ISIS except for the file handling that was not
} } designed for a multi user facility such as ours (approx 120 EM users in
} total
} } roughly 25 to 30 swapping every year). I am really quite impressed by the
} } INCA, Oxford Instruments are, at last, listening to the users and adding
} user
} } requested facilities. They have sorted out the file handling mess of the
} ISIS
} } and structured it well for an SEM user (not quite as well for a TEM user
} but
} } there are less of us). The software structure is quite intuitive and there
} is
} } a really impressive help menu and explanation of everything from the
} physics
} } of X-ray generation, how EM's work, how detectors work and how to analyze
} } samples.
} }
} } Their detectors have always been good and the SATW (thin window detectors)
} } still have a reasonable efficiency at low Z. B is possible but C is easy
} and
} } even the N peak is over 30% efficient (there is often a high absorption at
} N).
} }
} } Another feature that is invaluable for TEM is the integral shutter that
} will
} } close when the count rate is too high. This protects the crystal, it
} prevents
} } it overloading and shutting down or worse the crystal efficiency may
} change
} } for a few minutes until it recovers fully. This may affects your
} quantitative
} } work. In TEM this is usually caused by hitting the grid bar and not really
} a
} } problem in SEM but I don't know what secondaries and ions you will have in
} a
} } variable pressure SEM. It could be useful for you, check with other high
} } pressure SEM users.
} }
} } Regards,
} } Ron
} }
} } Please note: Oxford Instruments have upgraded an ISIS to an INCA system in
} my
} } department, without charge, in return for access to the instrument for
} } development projects and demonstrations for a fixed number of days. I
} receive
} } no benefit from this and the department has no benefit from Oxford
} Instruments
} } sales. I remain a thorn in the flesh of all our suppliers if I think they
} } could improve their products or service.
} } ron.doole-at-materials.oxford.ac.uk
} }
} }
} } --------------------------------------------------------------------------
} ----
} }
} }
} } Hello,
} } I am very familiar with the Oxford ISIS 300 series spectrometers. They are
} } ok, and the new Inca system looks good too. However, I recently saw the
} PGT
} } spectrometer at Lehigh and it is very impressive.
} }
} } Steve
} } Stephen_Skirius-at-bkitech.com
} }
} }
} }
} } --------------------------------------------------------------------------
} }
} }
} } I'm also in the market for an EDS system and have looked at EDAX, Noran,
} } PGT and Oxford.
} }
} } I edited out PGT because in order to quantify you have to optimize the
} } system for the type of sample by playing with fudge factors, which none of
} } the other systems have to do (though one of them, I think Noran, lets you
} } adjust a sensitivity factor if you want to, but they didn't do it on my
} } samples that were tested against known microprobe results and the answers
} } were fine). I also eliminated Oxford, though it has a terrific user
} } interface (maybe at the expense of functionality), because they
} } consistently IDed my aluminum peaks as Br or Tm (!); this made me wonder
} } about all their algorithms. They claim it had to do with the takeoff angle
} } on the particular SEM being used, but that shouldn't be a factor.
} }
} } I like both EDAX and Noran, though for different reasons. EDAX user
} } interface is better than Noran's, though again, I think Noran possibly
} } offers more routines (it's hard to keep track and see absolutely
} everything
} } a system has to offer in a demo day....).Noran can multitask - work on
} } several programs while a spectral map is being collected, for instance
} } (does EDAX? I have to check). But EDAX has a beam skirt reduction routine
} } for low vac mode (though it's time consuming, so a bit cumbersome), and
} } their peak modeling is right up front - but Noran can put theirs up front
} } also if you want to have it accessible (yes) and I think Noran might be a
} } little better engineered.
} }
} } As you can see I'm still in a quandary (ditto for the two contending SEMs,
} } LEO and ESEM). Whatever I decide I'll still be very interested in the
} } results of your posting - especially if other folks' info comes in within
} } the next week or so it would help in my decision too.
} }
} } I hope my input helps a little. Good luck with your quest!
} }
} } Dee Breger
} } micro-at-ldeo.columbia.edu
} }
} }
} } --------------------------------------------------------------------------
} -
} }
} }
} } I looked into Noran, EDAX, and PGT. Noran was quickly culled (less user
} } friendly, less abilities, didn't work right during demo), but EDAX and PGT
} } both seemed to have equivalent capabilities (PGT claimed a 'proprietary'
} } signal amplifier/digitizer doohickey, but it was only in the placement.)
} } For the long-time spectrum gathering (I forget the technical term), PGT
} } makes many passes with short dwell times while EDAX dwells on each pixel
} } much longer to collect data & does it in 1 pass. Kinda 6 of one, half
} dozen
} } of the other. What made us choose the EDAX Phoenix system was the fact
} that
} } the PGT software was UNIX-based (although hidden) while EDAX is PC-based.
} } I've heard rumors that PGT is switching to PC-based; we purchased our
} system
} } in 1999. I've also heard that Noran has practically no service techs (but
} } that may be an East coast thing.) We've been happy with the EDAX service,
} } and I enjoyed their user school very much. By the way, our SEM is a
} } variable pressure JEOL 5900, and it's integrated with the EDAX system.
} }
} } Hope I've been helpful,
} }
} } Jane L. LaGoy
} } Development Engineer
} } Bodycote IMT, Inc.
} } 155 River Street
} } Andover, MA 01810
} } 978-470-1620
} } jlagoy-at-bodycote-imt.com
} }
} }
} }
} }
}
}
}

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{HTML} {FONT FACE=arial,helvetica} Jean,
{BR}
{BR} I would caution biased opinions from installation engineer and sales
{BR} representatives ie... James Fotinopoulos.... www.semguy.com...
{BR}
{BR} Hmmmmmmmmm?
{BR}
{BR} Food for thought
{BR} {FONT SIZE=2}
{BR}
{BR}
{BR}
{BR}
{BR} ----- Original Message -----
{BR} {/FONT} {FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0"}
{BR} {/FONT} {FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0"} {BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"} From: "jeanross" <jeanross-at-blue.weeg.uiowa.edu>
{BR} To: "Microscopy Listserver" <Microscopy-at-sparc5.microscopy.com>
{BR} Sent: Tuesday, April 10, 2001 10:22 AM
{BR} Subject: EDS summary
{BR}
{BR}
{BR} > ------------------------------------------------------------------------
{BR} > The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} > To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} > On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} > -----------------------------------------------------------------------.
{BR} >
{BR} >
{BR} > I have put together a summary of responses I got from my inquiry about a
{BR} week
{BR} > or so ago about EDS systems.  I really appreciate everyone's input.  We
{BR} > haven't made any decisions yet since we are still gathering information
{BR} but
{BR} > your responses will help.  I've included the responses in their entirety
{BR} so I
{BR} > hope this helps others as well.
{BR} >
{BR} > Thanks again from everyone who contributed.
{BR} >
{BR} > Jean Ross
{BR} > Central Microscopy Research Facility
{BR} > University of Iowa
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} > I have been using an IXRF EDS / Gresham Detector (with IXRF digital pulse
{BR} > processor) system for almost 2 years. Initially, the IXRF was installed on
{BR} > an ETEC. About 15 months ago the Etec was replaced with a Hitachi 3500, a
{BR} > new detector (Gresham) was purchased, and the EDS was installed on the
{BR} 3500.
{BR} > Generally, I am quite satisfied. There are minor software bugs, but IXRF
{BR} > has been reasonably good at fixing them when discovered. It has been my
{BR} > experience that all the systems have bugs, perhaps some more than others.
{BR} > Prior to the IXRF, I had a Kevex 8000/Delta.
{BR} >
{BR} > Low end noise and broad peaks were evident on first installation, but were
{BR} > soon fixed by tweaking the detector preamp and pulse processor amp.
{BR} >
{BR} > I am still running their first software package, "Iridium". I have the
{BR} > newest release, "EDS 2000", but lack of time has kept me from installing
{BR} and
{BR} > checking it out.
{BR} >
{BR} > I should mention that IXRF is a "virtual" company, with people spread out
{BR} > between Texas, California, etc. This has not proven to be a problem.
{BR} >
{BR} > Woody White
{BR} > McDermott Technology, Inc.
{BR} > nwwhite-at-mcdermott.com
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > We have had EDAX for about a decade and a half, and we are very pleased
{BR} with
{BR} > the product and the service we get.
{BR} > Carol Heckman
{BR} > heckman-at-bgnet.bgsu.edu
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > look at IXRF eds systems there web site is www.ixrfsystems.com, they are
{BR} > very affordable and offer no nonsense performance that second to none.
{BR} >
{BR} > happy ixrf user,
{BR} >
{BR} > James Fotinopoulos
{BR} > yzfrjim-at-ix.netcom.com
{BR} >
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ----
{BR} >
{BR} > I would recommend you give consideration to Doug Connors at
{BR} > TN Analyzer Service, Inc. of Dane, WI. Doug has rebuild and
{BR} > upgraded detectors for me for the last 6 years. He is dependable
{BR} > knowledgeable, and economical as well.
{BR} >
{BR} > Bob Roberts
{BR} > EM Lab Services, Inc.
{BR} > 2409 S. Rural Rd Suite C
{BR} > Tempe, Arizona 85282
{BR} > (480) 967-3946
{BR} > bobrobs-at-earthlink.net
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > We recently purchased a Noran Instruments Vantage DS1.  We purchased this
{BR} > based on some very impressive demonstrations of software that the
{BR} salesperson
{BR} > brought in.  Unfortunately they are still working out the bugs in their
{BR} > software.  Everything they have is ported over from Unix, and literally
{BR} runs
{BR} > in a unix shell on an Microsoft Windows NT platform.  This makes their
{BR} > software fairly buggy.  Their response time to fix major bugs and hang-ups
{BR} in
{BR} > the software has been very slow, and if given the opportunity to do it all
{BR} > over again I'd probably look at Oxford Instruments.  I would still rate
{BR} the
{BR} > quality of the equipment very high.  Our detector performs at the
{BR} specified
{BR} > resolution, and is a good piece of equipment.  Now if they could only get
{BR} the
{BR} > software end of it straight...
{BR} > My vote:
{BR} > 1) Oxford Instruments
{BR} > 2)  Noran Instruments
{BR} > 3)  Edax or some of the smaller players
{BR} > The benefit with going with a larger company is support and upgrades.  We
{BR} have
{BR} > a 10 year old WDX that we just purchased new software and interface for
{BR} last
{BR} > year.  Our old EDS was given some trade in value by Noran.  And we all
{BR} know
{BR} > how valuable a service contract can be...
{BR} >
{BR} > Get back to me if you have any more questions,
{BR} > ~Jonathan
{BR} > Jonathan Dunlap
{BR} > Analytical Laboratory Manager
{BR} > Osram Sylvania Inc.
{BR} > 816 Lexington Avenue
{BR} > Warren, PA 16365
{BR} > Ph:  814-726-6991
{BR} > Fax: 814-726-6942
{BR} >  Jonathan.Dunlap-at-sylvania.com
{BR} >
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ----
{BR} >
{BR} >
{BR} > We have an Oxford Instruments Link ISIS Model 200 on our 2460N. We have
{BR} been
{BR} > happy with it, but I don't know about the direction that Oxford is
{BR} heading. I
{BR} > don't care for the feel of their new INCA software. Some might like it. It
{BR} > also seems to be slow coming together. Some of the functions are still
{BR} lacking
{BR} > after 2 (or is it 3) years of seeing it at MSA.
{BR} >
{BR} > We have an IXRF system on our JEOL 840A. It was a good price ($30K) for an
{BR} > upgrade to our Kevex several years ago. It does what we need. They keep at
{BR} > work on the software and have it freely available on the web. I might have
{BR} to
{BR} > pay closer attention and stay away from the beta stuff. They are still
{BR} working
{BR} > on it. They also have a nice digital pulse processor which stills stand
{BR} alone
{BR} > for about $5k.
{BR} >
{BR} > I still feel funny about some contacts with EVEX. I can't say much about
{BR} EDAX,
{BR} > NORAN, or PGT. They should all have good stuff but it might be pricey. The
{BR} > last we seriously looked at them was 6 years ago or so when we opted for
{BR} the
{BR} > Oxford.
{BR} >
{BR} > I was intrigued by the unit from Quartz PCI. I think it was called X-ray
{BR} One,
{BR} > or such. It was new at MSA 1-1/2 years ago but looked promising.
{BR} >
{BR} > Feel free to call if you want more details.
{BR} >
{BR} > Warren E Straszheim
{BR} > wesaia-at-iastate.edu
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > We purchased an EDAX Falcon system for our Hitachi S-3000N and I've been
{BR} > pleased with it. It has better light element sensitivity than most which
{BR} > was very important to me although I don't think that its mapping
{BR} > capabilities are as good as PGT's, say. I don't have direct experience
{BR} with
{BR} > Noran although I did talk to them and their system seemed ok - but
{BR} logistics
{BR} > didn't favor Noran so I passed on them. EDAX does have good integration
{BR} > with the Hitachi and the Quartz database.
{BR} >
{BR} > I'd be glad to respond more specifically if you'd like.
{BR} >
{BR} > Richard Shalvoy
{BR} > Arch Chemicals
{BR} > Cheshire, CT
{BR} > RBShalvoy-at-archchemicals.com
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > I have an iXRF systems out of Texas using a Gresham detector. It works
{BR} > well. Not the most cutting edge, but they are one of the "start ups". They
{BR} > have been around for I guess 6-7 years. I have a digital pulse processor
{BR} > and completely active control for x-ray maps and such. They are very price
{BR} > competitive, but lack a dedicated technical support person. You talk to
{BR} the
{BR} > programmer or electronics expert, but no techs on the phone whenever you
{BR} > have a software question. But, if you willing to wait a day for some
{BR} > answers then they are worth it. I haven't run across the problem where I
{BR} > thought, "if I just had a better system". If you want to integrated w/ WDS
{BR} > than maybe Noran. Also, if you want to integrated w/ motorized stage
{BR} > control, I don't think they off such a package, like the bigger companies.
{BR} >
{BR} > I have a Hitachi 450. I used to run a 2400 and 500 before I quit my day
{BR} job
{BR} > and went out on my own. I am very happy w/ Hitachi.
{BR} >
{BR} > Good Luck
{BR} >
{BR} > Fell free to call with any specifics.
{BR} >
{BR} > Their web page is www.ixrfsystems.com
{BR} >
{BR} > Ric
{BR} >
{BR} > SMARTech
{BR} > 860-491-3299
{BR} > www.semguy.com
{BR} > 19 Cornwall Drive
{BR} > Goshen CT 06756
{BR} > smartech-at-javanet.com
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ----
{BR} >
{BR} >
{BR} > Hello, all:
{BR} >
{BR} > I use Oxford ISIS300 system on HITACHI S-3500N (with VP mode) for light
{BR} > element analysis, mostly C, O, N, F, P, S, Si, Mg, as well as metal Co,
{BR} Ni-P,
{BR} > Pt, Cr, Fe, W, etc. This system works well. One useful function is the
{BR} overlay
{BR} > of 2 spectrums. I can easily subtract the blank from the sample spot and
{BR} make
{BR} > it easy to identify what is (are) in the sample. I am sure some other
{BR} program
{BR} > may have this kind of function, but I have not seen.
{BR} >
{BR} > Zhiyu Wang
{BR} > zhiyuw-at-home.com
{BR} > I would be interested in seeing the responses as I am going to try and get
{BR} > funding next year for a replacement for our EDAX PV9100 on an Hitachi
{BR} s-450.
{BR} >
{BR} > Dave
{BR} > David.Patton-at-uwe.ac.uk
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ---
{BR} >
{BR} >
{BR} > We have been running EDX on SEMs and TEMs for many years. We used to have
{BR} a
{BR} > range of systems from Kevex, PGT, Noran, Link, EDAX, however a few years
{BR} ago
{BR} > we decided that we ought to standardize on one common system. After
{BR} evaluation
{BR} > we bought three Oxford Instruments ISIS systems. Whenever we have upgraded
{BR} or
{BR} > bought new systems they have been Oxford Instruments ISIS or now INCA.
{BR} >
{BR} > I have been happy with the ISIS except for the file handling that was not
{BR} > designed for a multi user facility such as ours (approx 120 EM users in
{BR} total
{BR} > roughly 25 to 30 swapping every year). I am really quite impressed by the
{BR} > INCA, Oxford Instruments are, at last, listening to the users and adding
{BR} user
{BR} > requested facilities. They have sorted out the file handling mess of the
{BR} ISIS
{BR} > and structured it well for an SEM user (not quite as well for a TEM user
{BR} but
{BR} > there are less of us). The software structure is quite intuitive and there
{BR} is
{BR} > a really impressive help menu and explanation of everything from the
{BR} physics
{BR} > of X-ray generation, how EM's work, how detectors work and how to analyze
{BR} > samples.
{BR} >
{BR} > Their detectors have always been good and the SATW (thin window detectors)
{BR} > still have a reasonable efficiency at low Z. B is possible but C is easy
{BR} and
{BR} > even the N peak is over 30% efficient (there is often a high absorption at
{BR} N).
{BR} >
{BR} > Another feature that is invaluable for TEM is the integral shutter that
{BR} will
{BR} > close when the count rate is too high. This protects the crystal, it
{BR} prevents
{BR} > it overloading and shutting down or worse the crystal efficiency may
{BR} change
{BR} > for a few minutes until it recovers fully. This may affects your
{BR} quantitative
{BR} > work. In TEM this is usually caused by hitting the grid bar and not really
{BR} a
{BR} > problem in SEM but I don't know what secondaries and ions you will have in
{BR} a
{BR} > variable pressure SEM. It could be useful for you, check with other high
{BR} > pressure SEM users.
{BR} >
{BR} > Regards,
{BR} > Ron
{BR} >
{BR} > Please note: Oxford Instruments have upgraded an ISIS to an INCA system in
{BR} my
{BR} > department, without charge, in return for access to the instrument for
{BR} > development projects and demonstrations for a fixed number of days. I
{BR} receive
{BR} > no benefit from this and the department has no benefit from Oxford
{BR} Instruments
{BR} > sales. I remain a thorn in the flesh of all our suppliers if I think they
{BR} > could improve their products or service.
{BR} > ron.doole-at-materials.oxford.ac.uk
{BR} >
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} ----
{BR} >
{BR} >
{BR} > Hello,
{BR} > I am very familiar with the Oxford ISIS 300 series spectrometers. They are
{BR} > ok, and the new Inca system looks good too. However, I recently saw the
{BR} PGT
{BR} > spectrometer at Lehigh and it is very impressive.
{BR} >
{BR} > Steve
{BR} > Stephen_Skirius-at-bkitech.com
{BR} >
{BR} >
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} >
{BR} >
{BR} > I'm also in the market for an EDS system and have looked at EDAX, Noran,
{BR} > PGT and Oxford.
{BR} >
{BR} > I edited out PGT because in order to quantify you have to optimize the
{BR} > system for the type of sample by playing with fudge factors, which none of
{BR} > the other systems have to do (though one of them, I think Noran, lets you
{BR} > adjust a sensitivity factor if you want to, but they didn't do it on my
{BR} > samples that were tested against known microprobe results and the answers
{BR} > were fine). I also eliminated Oxford, though it has a terrific user
{BR} > interface (maybe at the expense of functionality), because they
{BR} > consistently IDed my aluminum peaks as Br or Tm (!); this made me wonder
{BR} > about all their algorithms. They claim it had to do with the takeoff angle
{BR} > on the particular SEM being used, but that shouldn't be a factor.
{BR} >
{BR} > I like both EDAX and Noran, though for different reasons. EDAX user
{BR} > interface is better than Noran's, though again, I think Noran possibly
{BR} > offers more routines (it's hard to keep track and see absolutely
{BR} everything
{BR} > a system has to offer in a demo day....).Noran can multitask - work on
{BR} > several programs while a spectral map is being collected, for instance
{BR} > (does EDAX? I have to check). But EDAX has a beam skirt reduction routine
{BR} > for low vac mode (though it's time consuming, so a bit cumbersome), and
{BR} > their peak modeling is right up front - but Noran can put theirs up front
{BR} > also if you want to have it accessible (yes) and I think Noran might be a
{BR} > little better engineered.
{BR} >
{BR} > As you can see I'm still in a quandary (ditto for the two contending SEMs,
{BR} > LEO and ESEM). Whatever I decide I'll still be very interested in the
{BR} > results of your posting - especially if other folks' info comes in within
{BR} > the next week or so it would help in my decision too.
{BR} >
{BR} > I hope my input helps a little. Good luck with your quest!
{BR} >
{BR} > Dee Breger
{BR} > micro-at-ldeo.columbia.edu
{BR} >
{BR} >
{BR} > --------------------------------------------------------------------------
{BR} -
{BR} >
{BR} >
{BR} > I looked into Noran, EDAX, and PGT. Noran was quickly culled (less user
{BR} > friendly, less abilities, didn't work right during demo), but EDAX and PGT
{BR} > both seemed to have equivalent capabilities (PGT claimed a 'proprietary'
{BR} > signal amplifier/digitizer doohickey, but it was only in the placement.)
{BR} > For the long-time spectrum gathering (I forget the technical term), PGT
{BR} > makes many passes with short dwell times while EDAX dwells on each pixel
{BR} > much longer to collect data & does it in 1 pass. Kinda 6 of one, half
{BR} dozen
{BR} > of the other. What made us choose the EDAX Phoenix system was the fact
{BR} that
{BR} > the PGT software was UNIX-based (although hidden) while EDAX is PC-based.
{BR} > I've heard rumors that PGT is switching to PC-based; we purchased our
{BR} system
{BR} > in 1999. I've also heard that Noran has practically no service techs (but
{BR} > that may be an East coast thing.) We've been happy with the EDAX service,
{BR} > and I enjoyed their user school very much. By the way, our SEM is a
{BR} > variable pressure JEOL 5900, and it's integrated with the EDAX system.
{BR} >
{BR} > Hope I've been helpful,
{BR} >
{BR} > Jane L. LaGoy
{BR} > Development Engineer
{BR} > Bodycote IMT, Inc.
{BR} > 155 River Street
{BR} > Andover, MA 01810
{BR} > 978-470-1620
{BR} > jlagoy-at-bodycote-imt.com
{BR} >
{BR} >
{BR} >
{BR} >
{BR}
{BR} {/XMP} {/FONT} {FONT COLOR="#0f0f0f" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0"}
{BR} {/FONT} {/HTML}

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I guess it was a slip:
In a sputter coater you can do rotary coating but shadowing is possible in
evaporators only.
For carbon coating the sputter coater would need an attachment for carbon
string evaporation, which may be used for SEM and analyses, but not in TEM.
Disclaimer: ProSciTech is the EMITECH instrument distributor in Australasia.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, April 11, 2001 5:18 AM, Gang Ning [SMTP:gning-at-mcw.edu] wrote:
}
}
} Hi All:
}
} I want to buy a new/used sputter coater which enables to do rotary
} shadowing as well as carbon coating. Any suggestions/input are
} appreciated.
}
} Greg Ning
}
} EM Facility
} Medical College of Wisconsin
}



From daemon Tue Apr 10 19:40:31 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Tue, 10 Apr 2001 17:35:26 -0700
Subject: RE: Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague and I each recently bought Microtek scanners to scan TEM negatives.
I have the Artixscan 1100 and he has the Model 8700 which has similar
characteristics (actually higher resolution -1200dpi), 3.9 dmax at 42 bits color
(14 grayscale), and the glassless film carrier setup. The 8700 has USB and
Firewire interfaces and is cheaper ( {$1000), and the 1000 dpi Model 1100 has a
SCSI interface. You might want to check out the specs of the lower cost model
8700 on the microtekusa website if your computer can handle USB or Firewire.
Both scanners have performed up to our expectations, which I would characterize
as modest. Microtek does not supply a 3-1/4 x 4 " negative carrier for standard
size TEM film but you can easily make a serviceable one from stiff paper or
light cardboard.

How much scanner resolution should you buy? The answer depends on how you
intend to use it. Most applications do not require capturing the full
resolution of the negative. From a practical viewpoint, the scanner resolution
just determines how many times you can magnify the negative image to produce the
final print size. For example, to get a publication-size print at 300 dpi, an
image scanned at 1200 dpi scan could be zoomed 4X. A practical alternative to
spending more for higher scanning resolution is to take photos at higher
magnification. One exception is with lattice images from the TEM, which
(depending on the lattice fringe spacing on the negative) might require higher
scan resolutions to avoid getting a moire effect. (Of course, not everyone
agrees. My colleague prefers to always scan at the maximum resolution).

What does a Dmax of 3.9 mean to you? To me it means a very dark negative. D is
the log of the transmitted to incident intensity ratio. I wonder if users ever
actually verify the manufacturer's specs with a calibrated density target. A
Dmax of 3.9 can be useful for scanning TEM diffraction patterns that might have
high contrast, but TEM micrograph negatives of metals and ceramics generally
don't have that much contrast and biological thin section photos tend to have
rather weak contrast. If your negatives are simply dark, use shorter photo
exposure times. Scanning with maximum allowed grayscale resolutions (e.g., 14
bits rather than 8) is highly recommended if you intend to enhance or adjust
images, but that's another story.


Larry Thomas
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto:Larry.Thomas-at-pnl.gov



----------
From: Tom Phillips
Sent: Tuesday, April 10, 2001 11:22 AM
To: Alwyn Eades
Cc: Microscopy-at-sparc5.microscopy.com
Subject: Re: Scanners

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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I too am about to buy and I would make a couple of comments on your
evaluation. First, let me remind everyone that the Dynamic range is
a log scale so small numerical differences are significant.

I also think the Nikon Coolscan 8000 looks great but it only takes a
2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers gone
to a smaller film size or is Nikon using a non-Japanese EM as their
standard? seems odd but I don't see how the Nikon would be very
useful. You say a {2000 line scanner would be useful 9 out of 10
times but want the 2000+ lines for the occasional high res scan. I
would argue that the size of the negative was the more important
variable to be worried about. The Nikon couldn't handle 4x5 LM
negatives or transparencies from autoradiography of
Westerns/Northerns, etc.

My leading candidate is the ArtixScan 1100 has a Dmax of 3.9 (about
$1600 with SCSI card). This was has a 1000 x 2000 dpi resolution.
more details at www.microtek.com. This is my leading candidate. It
was 4 negative carriers and I await word whether one could be
modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will have
my scientific instrumentation shop guys fabricate a holder. It comes
with a glass 8 x 10 glass carrier for odd size negs but I want to
avoid Newton rings and want a glassless carrier.

I would appreciate comments on the following argument (I think I have
this correctly figured out but am not sure since so many out there
seem to want to have a higher resolution scanner). I have a Fuji
Pictrography 3000 printer with a 400 dpi output that is as good as
any other widely available printer in the academic world. If you
figure the maximum published image size is about 8 inches, that would
mean the maximum image size be 3200 dpi wide. A 1000 dpi scan of my
negative would be 4500 x 3500 dpi. I could crop by about 28% or 10%
depending on the orientation of the negative and still be taking full
advantage of the printer resolution. In reality, most EM publication
prints are smaller than 8" wide so one could crop even more and still
not need more than 1000 dpi. A resolution } 1000 dpi would be
useful for subtle morphometric analysis but a 4000 dpi scan of a 3 x
4 negative would be 192 MB. That is pretty big for doing morphometry
on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16 MB
and that is much more manageable. Perhaps the difference is in the
type of EM we are doing. I am working with biological specimens
doing standard thin section type stuff. are you doing some Material
Sci application that demands more?


I will be interested in Alwyn (and any others) reply since I hope to
buy one soon!


} .
}
}
} There was a thread recently on scanners for TEM film. I have looked up
} all the models mentioned, on the web and called agents for prices - and
} produced a comparative table, given below.
}
} I do not guarantee that the figures are accurate but they are my best
} interpretation of the data given.
}
} In the light of experience and Nestor's comments, I would suggest that
} 2000 dpi is a minimum for TEM negatives. You may be able to get away
} with less nine times out of ten, but there will be occasions when you
} need more.
} I would exclude the Minolta and all the Epsons from consideration
} (despite the incredibly low prices of some of the Epsons) because of
the
} low pixel density.
}
} Among the rest the Nikon has the best pixel density and the best
optical
} density (another critical parameter for TEM negatives). The price is
} very competitive too. The Nikon web site does not give a time for
} scanning a negative. On the face of it the Nikon would be a best buy
-
} get a separate, inexpensive flatbed scanner for the other work.
}
} These comments are all my own opinions based on manufacturers' data.
} Since we are considering purchase any comments to the contrary would be
} most welcome.
}
}
}
} Code Maker Model Type
}
}
}
} A Agfa DuoScan T2500 Flatbed
} -Transparency included
}
} B Epson 1640 several versions Flatbed
} -Transparency option
} 1680 several versions
}
} C 1600 several versions Flatbed
} -Transparency included
}
} D Imacon Flextight Precision II Drum -for
} film and large format
}
} E Minolta Dimage ScanMulti II Film
}
} F Nikon Super Coolscan 8000ED Film
}
} G Polaroid 45 Ultra Film
}
} H Umax Powerlook 3000 Flatbed
} -Transparency included
}
}
}
}
}
} Code dpi OD Time Price
} Opinion
} at 6 x 9 cm
}
}
} A 2500 x2500 3.4 3 min
} $4,500 Fair
}
} B 1600 x 3200 3.6
} $300-$3000 Poor
}
} $800-$1400 Poor
}
} C 1600 x 3200 3.3
} $650-$1160 Not suitable
}
} D 2240 x2240** 3.9/4.1 N/A above
} $10k Good: low pixel density
}
} E 1128 x 1128 3.6
} Not suitable
}
} F 4000 x 4000 4.2 N/A
} $2,695 V. Good
}
} G 2500 x 2500 3.8 5 min
} $7,495 Good but pricey
}
} H 3048 x 3048 3.6 3 min
} $6,499 Good
}
}
} --
} ..........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)




From daemon Wed Apr 11 05:59:20 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Wed, 11 Apr 2001 05:47:58 -0500
Subject: RE: Scanners: quantitative accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Noise is only part of the problem, or solution, when considering a
quantitative approach to scanning in negative or positive images. Since
noise in electronic detection systems is largely dependent on temperature,
that must also be brought into consideration. But the characteristics of
the detector can be even more important. An array type device, such as a
CCD, can have characteristics that vary from pixel to pixel as sensitivity,
dynamic range and noise susceptibility. Your desire for a Consumer's
Report on scanners is probably most appropriate as these various effects
are difficult, if not impossible, to measure in a lab. Even if possible,
these measurements may not be extendable to similar machines that aren't
individually tested. Best to try to find a consensus on visual traits.

On Tuesday, April 10, 2001 7:50 AM, Sinkler, Wharton
[SMTP:WSinkler-at-uop.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Laurie and Gary,
}
} Would noise be a good criterion? Say, for a perfectly evenly darkened
film
} (if such a thing existed, or at least even on a scale { { collected pixel
} size) - what is the value of the noise (standard deviation of pixel
value)
} as a function of film darkness (density)?
}
} This would presumably improve with the time of collection. Thus how
"good"
} your scanner is depends on how you run it or whether it lets you take a
} slower scan or to average multiple scans. With the exception of drum
} scanners these devices all use CCD arrays. So what is probably most of
} interest is the signal to noise ratio as a function of illumination
} intensity, with everything known about CCD's going into determining this.
} The maximum density the scanner can handle is just the point at which the
} noise swamps the signal.
}
} There must be some good literature out there on the sources of noise,
} optimizing collection (scan) time etc. One article which might be a
} starting point is:
}
} G. H. Campbell, W. E. King and D. Cohen "Analysis of Experimental Error
in
} High Resolution Electron Micrographs", Microscopy and Microanalysis vol.
3
} (1997) p. 451.
}
} This is not very detailed, and treats only the total random noise, i.e.
} grouping noise arising in collecting the image with that arising from the
} scanner.
}
} Now, finding a good "Consumer Report" test with hard numbers on
commercial
} models is likely to be a lot harder!
}
} Wharton
}
} } -----Original Message-----
} } From: Gary Gaugler [SMTP:gary-at-gaugler.com]
} } Sent: Monday, April 09, 2001 10:37 PM
} } To: L. D. Marks
} } Cc: MSA listserver
} } Subject: Re: Scanners: quantitative accuracy
} }
} } --------------------------------------------------------------------
----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } How do you define "quantitative digitization?" i.e., what
} } variables are you dealing with in this respect? What are
} } the "absolute terms?"
} }
} } Anyone else have some ideas about this topic?
} }
} } gg
} }
} }
} } At 10:10 AM 4/9/2001, you wrote:
} }
} } } I have been listening to the thread on scanners. Has anyone done
} } } tests of how accurate they are in absolute terms for quantitative
} } } digitization?
} } }
} } } -------------------------------------------------------
} } } Laurence Marks
} } } Department of Materials Science and Engineering &
} } } Center for Transportation Nanotechnology
} } } Northwestern University
} } } Tel: (847) 491-3996 Fax: (847) 491-7820
} } } mailto:ldm-at-risc4.numis.nwu.edu
} } } http://www.numis.nwu.edu http://www.ctn.northwestern.edu
} }
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Wed Apr 11 09:02:40 2001



From: mckaylodge-at-aol.com ()
Date: Wed, 11 Apr 2001 08:59:24 -0500
Subject: Ask-A-Microscopist:Help Cleaning Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: mckaylodge-at-aol.com
Name: Robert Lodge

Organization: McKay Lodge Home School

Education: 9-12th Grade High School

Location: Oberlin, OH 44074

Question: My student accidently got immersion oil on the 40x Leica
Plan Acromat. I took a chance and used a fine artist's brush and
xylene to clean it recalling (I may be wrong) that lens adhesives are
soluble in alcohols not xylene, toluene and similar. Well, the lens
didn't fall out. Too bad because I need an excuse to upgrade! If (or
when) this happens again, what would you recommend for cleaning?

Bob Lodge

---------------------------------------------------------------------------


From daemon Wed Apr 11 09:02:43 2001



From: kunikova-at-mtf.stuba.sk ()
Date: Wed, 11 Apr 2001 08:58:46 -0500
Subject: Ask-A-Microscopist:TEM thinning of austenitic stainless

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: kunikova-at-mtf.stuba.sk
Name: terezia Kunikova

Organization: Faculty of Materils scince and technology, Slovak
University of Technology in Bratislava

Education: Undergraduate College

Location: Trnava, Slovakia

Question: Hi,
I am searching for suitable solution for final thinning of austenitic stainless
steel specimen for transmission electron microscopy.

Thank you.

---------------------------------------------------------------------------


From daemon Wed Apr 11 09:10:38 2001



From: DMoravits-at-swri.edu
Date: Wed, 11 Apr 2001 9:06:57 CDT
Subject: Microtome of Bone

Contents Retrieved from Microscopy Listserver Archives
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Does anyone out there have any experience microtoming bone that has NOT been
decalcified? Is it even possible? I'm hoping to be able to have an answer for
the admin people--prior to simply trying it out and possibly damaging my
diamond knife.

Don Moravits
Senior Technician
Southwest Research Institute
6220 Culebra Road
San Antonio, Texas 78238

Voice-210-522-2891
Fax-210-522-6220
E-Mail-dmoravits-at-swri.edu


From daemon Wed Apr 11 09:14:24 2001



From: timothy.quinn-at-tufts.edu
Date: Wed, 11 Apr 2001 10:09:52 -0400
Subject: Infiltration of feathers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everybody,

I am embedding feather barbs which are mostly spongey dry collagen with keratin
and small air pockets.

I am attempting Jan Dycks method-

1. 0.25 M NaOH 30 mins
2. formic acid / absolute alcohol 2:3 2 hrs ( to fill air pockets with
solution)
3. 15% epon / propylene oxide 3 days
4. standard graduated increase of epon / dehydrant

Any other suggestions? Possibly from other similar material such as plant
material.

Thanks

Tim Quinn
Kansas University
Museum of Natural History
Lawrence, KS
785-864-4556


From daemon Wed Apr 11 10:30:31 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 11 Apr 2001 10:22:19 -0500
Subject: RE: Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would love to take advantage of the Firewire option but my
information is that the 8700 has a Dmax of 3.4 vs the 3.9 for the
1100. That is a significant difference. Do EM negatives of
biological thin sections reach that? I think so. I do a lot of EM
immunocytochemistry and have to look for gold (intensely black)
against a very dark tissue component so I am hoping the higher Dmax
improves my results. I frequently scan negatives on a Umax 1100
(Dmax 3.4??) and can't differentiate the gold from the background
although by eye I can discriminate them when the negative is placed
on a light box. Changing my exposure would give me an unuseable
image for the rest of the tissue. Maybe this is an extreme case but
I suspect that lots of "dark organelles" (e.g., lysosomes, nuclei)
have fine structure that get lost in the scanning with a low Dmax
scanner. Tom


} A colleague and I each recently bought Microtek scanners to scan TEM
} negatives.
} I have the Artixscan 1100 and he has the Model 8700 which has similar
} characteristics (actually higher resolution -1200dpi), 3.9 dmax at
} 42 bits color
} (14 grayscale), and the glassless film carrier setup. The 8700 has USB and
} Firewire interfaces and is cheaper ( {$1000), and the 1000 dpi Model 1100 has a
} SCSI interface. You might want to check out the specs of the lower cost model
} 8700 on the microtekusa website if your computer can handle USB or Firewire.
} Both scanners have performed up to our expectations, which I would
} characterize
} as modest. Microtek does not supply a 3-1/4 x 4 " negative carrier
} for standard
} size TEM film but you can easily make a serviceable one from stiff paper or
} light cardboard.
}
} How much scanner resolution should you buy? The answer depends on how you
} intend to use it. Most applications do not require capturing the full
} resolution of the negative. From a practical viewpoint, the scanner
} resolution
} just determines how many times you can magnify the negative image to
} produce the
} final print size. For example, to get a publication-size print at 300 dpi, an
} image scanned at 1200 dpi scan could be zoomed 4X. A practical alternative to
} spending more for higher scanning resolution is to take photos at higher
} magnification. One exception is with lattice images from the TEM, which
} (depending on the lattice fringe spacing on the negative) might require higher
} scan resolutions to avoid getting a moire effect. (Of course, not everyone
} agrees. My colleague prefers to always scan at the maximum resolution).
}
} What does a Dmax of 3.9 mean to you? To me it means a very dark
} negative. D is
} the log of the transmitted to incident intensity ratio. I wonder if
} users ever
} actually verify the manufacturer's specs with a calibrated density target. A
} Dmax of 3.9 can be useful for scanning TEM diffraction patterns that
} might have
} high contrast, but TEM micrograph negatives of metals and ceramics generally
} don't have that much contrast and biological thin section photos tend to have
} rather weak contrast. If your negatives are simply dark, use shorter photo
} exposure times. Scanning with maximum allowed grayscale resolutions (e.g., 14
} bits rather than 8) is highly recommended if you intend to enhance or adjust
} images, but that's another story.
}
}
} Larry Thomas
} Pacific Northwest National Laboratory
} MSIN P8-16
} P.O. Box 999
} Richland, WA 99352
} Phone: (509)372-0793 Fax: (509)376-6308
} Email: mailto:Larry.Thomas-at-pnl.gov
}
}
}
} ----------
} From: Tom Phillips
} Sent: Tuesday, April 10, 2001 11:22 AM
} To: Alwyn Eades
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Scanners
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
}
}
} I too am about to buy and I would make a couple of comments on your
} evaluation. First, let me remind everyone that the Dynamic range is
} a log scale so small numerical differences are significant.
}
} I also think the Nikon Coolscan 8000 looks great but it only takes a
} 2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
} negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers gone
} to a smaller film size or is Nikon using a non-Japanese EM as their
} standard? seems odd but I don't see how the Nikon would be very
} useful. You say a {2000 line scanner would be useful 9 out of 10
} times but want the 2000+ lines for the occasional high res scan. I
} would argue that the size of the negative was the more important
} variable to be worried about. The Nikon couldn't handle 4x5 LM
} negatives or transparencies from autoradiography of
} Westerns/Northerns, etc.
}
} My leading candidate is the ArtixScan 1100 has a Dmax of 3.9 (about
} $1600 with SCSI card). This was has a 1000 x 2000 dpi resolution.
} more details at www.microtek.com. This is my leading candidate. It
} was 4 negative carriers and I await word whether one could be
} modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will have
} my scientific instrumentation shop guys fabricate a holder. It comes
} with a glass 8 x 10 glass carrier for odd size negs but I want to
} avoid Newton rings and want a glassless carrier.
}
} I would appreciate comments on the following argument (I think I have
} this correctly figured out but am not sure since so many out there
} seem to want to have a higher resolution scanner). I have a Fuji
} Pictrography 3000 printer with a 400 dpi output that is as good as
} any other widely available printer in the academic world. If you
} figure the maximum published image size is about 8 inches, that would
} mean the maximum image size be 3200 dpi wide. A 1000 dpi scan of my
} negative would be 4500 x 3500 dpi. I could crop by about 28% or 10%
} depending on the orientation of the negative and still be taking full
} advantage of the printer resolution. In reality, most EM publication
} prints are smaller than 8" wide so one could crop even more and still
} not need more than 1000 dpi. A resolution } 1000 dpi would be
} useful for subtle morphometric analysis but a 4000 dpi scan of a 3 x
} 4 negative would be 192 MB. That is pretty big for doing morphometry
} on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16 MB
} and that is much more manageable. Perhaps the difference is in the
} type of EM we are doing. I am working with biological specimens
} doing standard thin section type stuff. are you doing some Material
} Sci application that demands more?
}
}
} I will be interested in Alwyn (and any others) reply since I hope to
} buy one soon!
}
}
} } .
} }
} }
} } There was a thread recently on scanners for TEM film. I
} have looked up
} } all the models mentioned, on the web and called agents for
} prices - and
} } produced a comparative table, given below.
} }
} } I do not guarantee that the figures are accurate but they are my best
} } interpretation of the data given.
} }
} } In the light of experience and Nestor's comments, I would suggest that
} } 2000 dpi is a minimum for TEM negatives. You may be able to get away
} } with less nine times out of ten, but there will be occasions when you
} } need more.
} } I would exclude the Minolta and all the Epsons from consideration
} } (despite the incredibly low prices of some of the Epsons) because of
} the
} } low pixel density.
} }
} } Among the rest the Nikon has the best pixel density and the best
} optical
} } density (another critical parameter for TEM negatives). The price is
} } very competitive too. The Nikon web site does not give a time for
} } scanning a negative. On the face of it the Nikon would be a best buy
} -
} } get a separate, inexpensive flatbed scanner for the other work.
} }
} } These comments are all my own opinions based on manufacturers' data.
} } Since we are considering purchase any comments to the
} contrary would be
} } most welcome.
} }
} }
} }
} } Code Maker Model Type
} }
} }
} }
} } A Agfa DuoScan T2500 Flatbed
} } -Transparency included
} }
} } B Epson 1640 several versions Flatbed
} } -Transparency option
} } 1680 several versions
} }
} } C 1600 several versions Flatbed
} } -Transparency included
} }
} } D Imacon Flextight Precision II Drum -for
} } film and large format
} }
} } E Minolta Dimage ScanMulti II Film
} }
} } F Nikon Super Coolscan 8000ED Film
} }
} } G Polaroid 45 Ultra Film
} }
} } H Umax Powerlook 3000 Flatbed
} } -Transparency included
} }
} }
} }
} }
} }
} } Code dpi OD Time Price
} } Opinion
} } at 6 x 9 cm
} }
} }
} } A 2500 x2500 3.4 3 min
} } $4,500 Fair
} }
} } B 1600 x 3200 3.6
} } $300-$3000 Poor
} }
} } $800-$1400 Poor
} }
} } C 1600 x 3200 3.3
} } $650-$1160 Not suitable
} }
} } D 2240 x2240** 3.9/4.1 N/A above
} } $10k Good: low pixel density
} }
} } E 1128 x 1128 3.6
} } Not suitable
} }
} } F 4000 x 4000 4.2 N/A
} } $2,695 V. Good
} }
} } G 2500 x 2500 3.8 5 min
} } $7,495 Good but pricey
} }
} } H 3048 x 3048 3.6 3 min
} } $6,499 Good
} }
} }
} } --
} } ..........
} } Alwyn Eades
} } Department of Materials Science and Engineering
} } Lehigh University
} } 5 East Packer Avenue
} } Bethlehem
} } Pennsylvania 18015-3195
} } Phone 610 758 4231
} } Fax 610 758 4244
} } jae5-at-lehigh.edu
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Apr 11 10:43:35 2001



From: Connie A Cummings/students/Cvm :      rosscac-at-cvm.okstate.edu
Date: Wed, 11 Apr 2001 10:39:08 -0500
Subject: multiple staining grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know where I can find a multiple staining device for TEM grids
using UA and Reynolds lead citrate that will result in CLEAN grids.
Thank you
Connie
rosscac-at-okstate.edu



From daemon Wed Apr 11 11:23:40 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 11 Apr 2001 09:14:27 -0700
Subject: Re: Ask-A-Microscopist: microscope kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Name: Kade
} Organization: RMIT Melbourne Australia
} Education: Graduate College
} Location: Melbourne, Victoria, Australia
}
} Question: Hi, I have just purchased a microscope, a good biological
} one. I need a microscope kit but nobody sells them around here.
} Anyway already I have glass slides and covers. I have read some on
} microscopy and I need an adhesive, resin I think its called to
} prepare slides? is this true?
} Also some ink to stain specimens. What are the names of all these
} chemicals so I can buy them all seperatly since no one sells them all
} together.

Kade -

You need a book on "microtechnique" plus a general idea of the types of
specimens that you want to look at, BEFORE you start ordering things. Even
tho you've "read some on microscopy " you could start with this book:

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy. Almost half of the
book is devoted to simple preparation methods for biological specimens and
descriptions (with gool illustrations) of commonly encountered organisms.
Adult. RECOMMENDED

You will find a lot of help on the amateur microscopy website
http://www.microscopy-uk.org.uk

Both of these listings are from the Project MICRO bibliography (URL below).


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Apr 11 12:04:18 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Wed, 11 Apr 2001 09:58:46 -0700
Subject: RE: Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Your information is correct and mine is not. The Dmax of the 8700 is 3.4.



Larry

----------
From: Tom Phillips
Sent: Wednesday, April 11, 2001 8:22 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: jae5-at-lehigh.edu; Thomas, Larry (PNNL)
Subject: RE: Scanners

I would love to take advantage of the Firewire option but my
information is that the 8700 has a Dmax of 3.4 vs the 3.9 for the
1100. That is a significant difference. Do EM negatives of
biological thin sections reach that? I think so. I do a lot of EM
immunocytochemistry and have to look for gold (intensely black)
against a very dark tissue component so I am hoping the higher Dmax
improves my results. I frequently scan negatives on a Umax 1100
(Dmax 3.4??) and can't differentiate the gold from the background
although by eye I can discriminate them when the negative is placed
on a light box. Changing my exposure would give me an unuseable
image for the rest of the tissue. Maybe this is an extreme case but
I suspect that lots of "dark organelles" (e.g., lysosomes, nuclei)
have fine structure that get lost in the scanning with a low Dmax
scanner. Tom


} A colleague and I each recently bought Microtek scanners to scan TEM
} negatives.
} I have the Artixscan 1100 and he has the Model 8700 which has similar
} characteristics (actually higher resolution -1200dpi), 3.9 dmax at
} 42 bits color
} (14 grayscale), and the glassless film carrier setup. The 8700 has USB
and
} Firewire interfaces and is cheaper ( {$1000), and the 1000 dpi Model
1100 has a
} SCSI interface. You might want to check out the specs of the lower
cost model
} 8700 on the microtekusa website if your computer can handle USB or
Firewire.
} Both scanners have performed up to our expectations, which I would
} characterize
} as modest. Microtek does not supply a 3-1/4 x 4 " negative carrier
} for standard
} size TEM film but you can easily make a serviceable one from stiff
paper or
} light cardboard.
}
} How much scanner resolution should you buy? The answer depends on how
you
} intend to use it. Most applications do not require capturing the full
} resolution of the negative. From a practical viewpoint, the scanner
} resolution
} just determines how many times you can magnify the negative image to
} produce the
} final print size. For example, to get a publication-size print at 300
dpi, an
} image scanned at 1200 dpi scan could be zoomed 4X. A practical
alternative to
} spending more for higher scanning resolution is to take photos at
higher
} magnification. One exception is with lattice images from the TEM,
which
} (depending on the lattice fringe spacing on the negative) might require
higher
} scan resolutions to avoid getting a moire effect. (Of course, not
everyone
} agrees. My colleague prefers to always scan at the maximum resolution).
}
} What does a Dmax of 3.9 mean to you? To me it means a very dark
} negative. D is
} the log of the transmitted to incident intensity ratio. I wonder if
} users ever
} actually verify the manufacturer's specs with a calibrated density
target. A
} Dmax of 3.9 can be useful for scanning TEM diffraction patterns that
} might have
} high contrast, but TEM micrograph negatives of metals and ceramics
generally
} don't have that much contrast and biological thin section photos tend
to have
} rather weak contrast. If your negatives are simply dark, use shorter
photo
} exposure times. Scanning with maximum allowed grayscale resolutions
(e.g., 14
} bits rather than 8) is highly recommended if you intend to enhance or
adjust
} images, but that's another story.
}
}
} Larry Thomas
} Pacific Northwest National Laboratory
} MSIN P8-16
} P.O. Box 999
} Richland, WA 99352
} Phone: (509)372-0793 Fax: (509)376-6308
} Email: mailto:Larry.Thomas-at-pnl.gov
}
}
}
} ----------
} From: Tom Phillips
} Sent: Tuesday, April 10, 2001 11:22 AM
} To: Alwyn Eades
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Scanners
}
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
}
-----------------------------------------------------------------------.
}
}
} I too am about to buy and I would make a couple of comments on
your
} evaluation. First, let me remind everyone that the Dynamic
range is
} a log scale so small numerical differences are significant.
}
} I also think the Nikon Coolscan 8000 looks great but it only
takes a
} 2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
} negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers
gone
} to a smaller film size or is Nikon using a non-Japanese EM as
their
} standard? seems odd but I don't see how the Nikon would be very
} useful. You say a {2000 line scanner would be useful 9 out of
10
} times but want the 2000+ lines for the occasional high res scan.
I
} would argue that the size of the negative was the more important
} variable to be worried about. The Nikon couldn't handle 4x5 LM
} negatives or transparencies from autoradiography of
} Westerns/Northerns, etc.
}
} My leading candidate is the ArtixScan 1100 has a Dmax of 3.9
(about
} $1600 with SCSI card). This was has a 1000 x 2000 dpi
resolution.
} more details at www.microtek.com. This is my leading candidate.
It
} was 4 negative carriers and I await word whether one could be
} modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will
have
} my scientific instrumentation shop guys fabricate a holder. It
comes
} with a glass 8 x 10 glass carrier for odd size negs but I want
to
} avoid Newton rings and want a glassless carrier.
}
} I would appreciate comments on the following argument (I think I
have
} this correctly figured out but am not sure since so many out
there
} seem to want to have a higher resolution scanner). I have a
Fuji
} Pictrography 3000 printer with a 400 dpi output that is as good
as
} any other widely available printer in the academic world. If
you
} figure the maximum published image size is about 8 inches, that
would
} mean the maximum image size be 3200 dpi wide. A 1000 dpi scan
of my
} negative would be 4500 x 3500 dpi. I could crop by about 28% or
10%
} depending on the orientation of the negative and still be taking
full
} advantage of the printer resolution. In reality, most EM
publication
} prints are smaller than 8" wide so one could crop even more and
still
} not need more than 1000 dpi. A resolution } 1000 dpi would be
} useful for subtle morphometric analysis but a 4000 dpi scan of a
3 x
} 4 negative would be 192 MB. That is pretty big for doing
morphometry
} on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16
MB
} and that is much more manageable. Perhaps the difference is in
the
} type of EM we are doing. I am working with biological specimens
} doing standard thin section type stuff. are you doing some
Material
} Sci application that demands more?
}
}
} I will be interested in Alwyn (and any others) reply since I
hope to
} buy one soon!
}
}
} } .
} }
} }
} } There was a thread recently on scanners for TEM film. I
} have looked up
} } all the models mentioned, on the web and called agents for
} prices - and
} } produced a comparative table, given below.
} }
} } I do not guarantee that the figures are accurate but they are
my best
} } interpretation of the data given.
} }
} } In the light of experience and Nestor's comments, I would
suggest that
} } 2000 dpi is a minimum for TEM negatives. You may be able to get
away
} } with less nine times out of ten, but there will be occasions
when you
} } need more.
} } I would exclude the Minolta and all the Epsons from
consideration
} } (despite the incredibly low prices of some of the Epsons)
because of
} the
} } low pixel density.
} }
} } Among the rest the Nikon has the best pixel density and the
best
} optical
} } density (another critical parameter for TEM negatives). The
price is
} } very competitive too. The Nikon web site does not give a time
for
} } scanning a negative. On the face of it the Nikon would be a
best buy
} -
} } get a separate, inexpensive flatbed scanner for the other work.
} }
} } These comments are all my own opinions based on manufacturers'
data.
} } Since we are considering purchase any comments to the
} contrary would be
} } most welcome.
} }
} }
} }
} } Code Maker Model Type
} }
} }
} }
} } A Agfa DuoScan T2500 Flatbed
} } -Transparency included
} }
} } B Epson 1640 several versions Flatbed
} } -Transparency option
} } 1680 several versions
} }
} } C 1600 several versions Flatbed
} } -Transparency included
} }
} } D Imacon Flextight Precision II Drum
-for
} } film and large format
} }
} } E Minolta Dimage ScanMulti II Film
} }
} } F Nikon Super Coolscan 8000ED Film
} }
} } G Polaroid 45 Ultra Film
} }
} } H Umax Powerlook 3000 Flatbed
} } -Transparency included
} }
} }
} }
} }
} }
} } Code dpi OD Time
Price
} } Opinion
} } at 6 x 9 cm
} }
} }
} } A 2500 x2500 3.4 3 min
} } $4,500 Fair
} }
} } B 1600 x 3200 3.6
} } $300-$3000 Poor
} }
} } $800-$1400 Poor
} }
} } C 1600 x 3200 3.3
} } $650-$1160 Not suitable
} }
} } D 2240 x2240** 3.9/4.1 N/A
above
} } $10k Good: low pixel density
} }
} } E 1128 x 1128 3.6
} } Not suitable
} }
} } F 4000 x 4000 4.2 N/A
} } $2,695 V. Good
} }
} } G 2500 x 2500 3.8 5 min
} } $7,495 Good but pricey
} }
} } H 3048 x 3048 3.6 3 min
} } $6,499 Good
} }
} }
} } --
} } ..........
} } Alwyn Eades
} } Department of Materials Science and Engineering
} } Lehigh University
} } 5 East Packer Avenue
} } Bethlehem
} } Pennsylvania 18015-3195
} } Phone 610 758 4231
} } Fax 610 758 4244
} } jae5-at-lehigh.edu
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)




From daemon Wed Apr 11 13:05:12 2001



From: christine :      ac.richardson2-at-btinternet.com
Date: Wed, 11 Apr 2001 18:59:24 +0100
Subject: Autoradiograph film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
We are having a great deal of difficulty getting hold of our normal film
"Tritiated Hyper film" for use with tritiated ligands.
Does any one out there know of a local supplier (U.K.)

Thanks in advance,

P.S Thank you Nestor for all your help.



From daemon Wed Apr 11 16:06:42 2001



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 11 Apr 2001 17:00:20 -0400 (EDT)
Subject: Re: Ask-A-Microscopist:Help Cleaning Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robert,

We use 70 % isopropanol to clean emersion oil off of our lenses.

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Wed, 11 Apr 2001 mckaylodge-at-aol.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Email: mckaylodge-at-aol.com
} Name: Robert Lodge
}
} Organization: McKay Lodge Home School
}
} Education: 9-12th Grade High School
}
} Location: Oberlin, OH 44074
}
} Question: My student accidently got immersion oil on the 40x Leica
} Plan Acromat. I took a chance and used a fine artist's brush and
} xylene to clean it recalling (I may be wrong) that lens adhesives are
} soluble in alcohols not xylene, toluene and similar. Well, the lens
} didn't fall out. Too bad because I need an excuse to upgrade! If (or
} when) this happens again, what would you recommend for cleaning?
}
} Bob Lodge
}
} ---------------------------------------------------------------------------
}



From daemon Wed Apr 11 17:13:51 2001



From: Richard Gardiner :      rbgardiner-at-home.com
Date: Wed, 11 Apr 2001 18:08:40 -0400
Subject: Re: Particle Concentration Determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone had experience in determining the concentration of particles
in a solution by counting on EM grids in a similar way to using a
haemocytometer? Is it possible to count the particles in a defined
number of grid squares then calculate back to the area of the grid and
the amount of solution which was applied and allowed to dry down?

Richard Gardiner
Department of Plant Sciences
University of Western Ontario



From daemon Wed Apr 11 17:30:56 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 11 Apr 2001 15:25:01 -0700
Subject: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Just tried to order some Kodabrome II RC paper, grade F5. Vendor says it is
discontinued. Any suggestions for an alternative? We have been using
Kodabrome RC II for a long time with a Mohr processor. How about
Polycontrast RC? Or is this the beginning of the end for old fashioned
darkrooms?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Apr 11 17:45:27 2001



From: Bernard Kestel :      kestel-at-anl.gov
Date: 11 Apr 01 17:41:01 -0600
Subject: Film Processing for Computer Scanners:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



One of the big advantages of computer printing of TEM images is the
ability to adjust the contrast of the final image. However, to prevent
generating a negative that has completely overexposed areas which are
difficult to salvage by any means-such as one may get with metal or ceramic
specimens-an alternative film developer may help.
There are two bath "split" developers which lower contrast to
manageable levels, in effect chemically "dodging" a developing negative. Fixing is
carried out normally. I'm told biological specimens don't usually need
such treatment.
I have no financial interest in the following company, but I am
pleased with the results obtained with their developer called Diafine. It is
made by Acufine Inc., 5441 North Kedzie Ave. Chicago, Il., 60625.

Bernard Kestel E-mail: {kestel-at-anl.gov}
Materials Science Division
Argonne National Laboratory
Argonne, Il., 60439



From daemon Wed Apr 11 19:15:22 2001



From: jgh7f0-at-mizzou.edu ()
Date: Wed, 11 Apr 2001 19:12:36 -0500
Subject: Ask-A-Microscopist: Looking for image of S. Agalactiae

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(jgh7f0-at-mizzou.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
April 11, 2001 at 14:32:21
---------------------------------------------------------------------------

Email: jgh7f0-at-mizzou.edu
Name: john harris

Organization: university of missouri-columbia

Education: Undergraduate College

Location: Columbia, Missouri

Question: Dear Sir or Madam;
i am looking for an image of S. Agalactiae attached to
an epithelial cell or some other gram + cocci.

thank you for your time, enery and efforts.

sincerely,
jgh

---------------------------------------------------------------------------


From daemon Wed Apr 11 20:50:45 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 11 Apr 2001 18:52:05 -0700
Subject: Re: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kodabrome is for sure an old product. But a good one.
I quit using it long ago in favor of Ilford paper. Ilford
makes RC paper--but my work has only been output to archival
fiber paper. But I suspect that the RC quality is still up to par.

I use plain matte for normal prints--and archival matte fiber
for fine art prints (nudes, etc.). I use a Kreonite processor
for the print papers. All b/w neg media is hand processed
in separate roll holders (Nikor). My normal format is 6x4.5cm
and 6x7cm. The same recipes would apply to other formats.

I did some 4x5" work in prior years and do the same
mechanics today.

Try some Ilford paper.

gary g.

http://photoweb.net



At 03:25 PM 4/11/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Apr 11 21:20:26 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 11 Apr 2001 19:21:51 -0700
Subject: Re: Film Processing for Computer Scanners:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wow...this is really deja vu. I used Diafine and Acufine
way many years ago. At that time, the issue was push
processing. Today, the zone system prevails. Using the
zone system does not require special chemicals. It is a
matter of how the neg is rated and how it is processed.

Look up some of Ansel Adams' works (The Negative).
The main idea is to adjust your neg's EV for total tonal
range such that it can be printed with less than bone
crushing effort.

All of my fine art prints are shot and printed using this
process.

gary g.

http://photoweb.net


At 04:41 PM 4/11/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Apr 12 00:16:02 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 12 Apr 2001 00:10:54 -0500
Subject: Re: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I used Illiford RC paper for a long time and I liked it better than
anything else I have ever used. It may be just be personal preference but
it seemed to have brighter higlights and blacker blacks than I could get
with other RC papers. Just be sure and get Illford filters to go with it.
One other nice thing is it has a matt finsh as well as glossy if you don't
want glossy prints. The matt will scan great. It doesn't have texture
problems like most pearl or simi-glossy papers have. The matt finish
displays a lot better than glossy finsh IMHO.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger


} From: "Gary Gaugler" {gary-at-gaugler.com}

} Kodabrome is for sure an old product. But a good one.
} I quit using it long ago in favor of Ilford paper. Ilford
} makes RC paper--but my work has only been output to archival
} fiber paper. But I suspect that the RC quality is still up to par.
}
} I use plain matte for normal prints--and archival matte fiber
} for fine art prints (nudes, etc.). I use a Kreonite processor
} for the print papers. All b/w neg media is hand processed
} in separate roll holders (Nikor). My normal format is 6x4.5cm
} and 6x7cm. The same recipes would apply to other formats.
}
} I did some 4x5" work in prior years and do the same
} mechanics today.
}
} Try some Ilford paper.
}
} gary g.
}
} http://photoweb.net
}
}
}
} } Just tried to order some Kodabrome II RC paper, grade F5. Vendor says
it is
} } discontinued. Any suggestions for an alternative? We have been using
} } Kodabrome RC II for a long time with a Mohr processor. How about
} } Polycontrast RC? Or is this the beginning of the end for old fashioned
} } darkrooms?
} }
} } Jonathan Krupp





From daemon Thu Apr 12 00:33:54 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 11 Apr 2001 22:34:01 -0700
Subject: Fwd: Re: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am using Polymax II RC. For draft pictures it's good enough in my point
} of view. In compare with Ilford Multigrade III RC, Polymax gives better
} contrast (filter #5 on Ilford is equal to #3 on Polymax in my hands).


Sergey



} At 03:25 PM 4/11/2001, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Apr 12 03:55:55 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 12 Apr 2001 03:07:33 -0500
Subject: Re: Film Processing for Computer Scanners:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Usualy to reduce contrast you under expose the film. Then develop it to
the disired density using a developer that generates low contrast. One way
to reduce contrast is to dilute you developer by a factor of 2, 4 or more
with water. It extends the developing time a good deal but it reduces the
contrast. You might also look at low contrast developers that work with
the film you are using.

A few question on rec.photo.darkroom will get you more information than
you can handle and some of it will actually work.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} }
} }
} } One of the big advantages of computer printing of TEM images is the
} } ability to adjust the contrast of the final image. However, to prevent
} } generating a negative that has completely overexposed areas which are
} } difficult to salvage by any means-such as one may get with metal or
ceramic
} } specimens-an alternative film developer may help.
} } There are two bath "split" developers which lower contrast to
} } manageable levels, in effect chemically "dodging" a developing
negative.
} } Fixing is
} } carried out normally. I'm told biological specimens don't usually need
} } such treatment.
} } I have no financial interest in the following company, but I am
} } pleased with the results obtained with their developer called Diafine.
It is
} } made by Acufine Inc., 5441 North Kedzie Ave. Chicago, Il., 60625.
} }
} } Bernard Kestel E-mail: {kestel-at-anl.gov}
} } Materials Science Division
} } Argonne National Laboratory
} } Argonne, Il., 60439
}
}




From daemon Thu Apr 12 07:07:31 2001



From: Michelle.Taurino-at-aventis.com
Date: Thu, 12 Apr 2001 06:56:04 -0500
Subject: Autoradiograph film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have your tried Amersham} ??

Michelle Taurino
Aventis Pharmaceuticals
Senior Scientist
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: christine [mailto:ac.richardson2-at-btinternet.com]
Sent: Wednesday, April 11, 2001 1:59 PM
To: microscopy-at-sparc5.microscopy.com


Dear all,
We are having a great deal of difficulty getting hold of our normal
film
"Tritiated Hyper film" for use with tritiated ligands.
Does any one out there know of a local supplier (U.K.)

Thanks in advance,

P.S Thank you Nestor for all your help.


From daemon Thu Apr 12 07:58:57 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 12 Apr 2001 07:56:48 -0500
Subject: RE: Ask-A-Microscopist:Help Cleaning Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robert:

Back in the pre PC (chemical) days, toluene was a recommended
solvent for cleaning lenses of immersion oil. Now, we at National Steel,
wipe off excess oil with lens tissue and then clean the lens with Kodak lens
cleaner solution.

Best regards,

Sam Purdy
National Steel Tech Center
Trenton MI

} ----------
} From: mckaylodge-at-aol.com
} Sent: Wednesday, April 11, 2001 9:59 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:Help Cleaning Lenses
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Email: mckaylodge-at-aol.com
} Name: Robert Lodge
}
} Organization: McKay Lodge Home School
}
} Education: 9-12th Grade High School
}
} Location: Oberlin, OH 44074
}
} Question: My student accidently got immersion oil on the 40x Leica
} Plan Acromat. I took a chance and used a fine artist's brush and
} xylene to clean it recalling (I may be wrong) that lens adhesives are
} soluble in alcohols not xylene, toluene and similar. Well, the lens
} didn't fall out. Too bad because I need an excuse to upgrade! If (or
} when) this happens again, what would you recommend for cleaning?
}
} Bob Lodge
}
} --------------------------------------------------------------------------
} -
}


From daemon Thu Apr 12 08:30:45 2001



From: christine :      ac.richardson2-at-btinternet.com
Date: Thu, 12 Apr 2001 14:24:57 +0100
Subject: Autoradiograph film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have always used Amersham but they have now discontinued making it.
Does anyone know of a replacement for it.
Christine.


From daemon Thu Apr 12 08:54:30 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 12 Apr 2001 09:48:41 -0700
Subject: RE: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kodabrome has not been discontinued across the board. However, certain
size/surface/grade combinations have been. Kodabrome II RC F5 is available
in 250sheet 8x10 packages only. We stock them but a dealer should be able
to order them.
As far as alternatives....
Agfa offers Brovira Speed RC, a developer incorporated paper in grades 2-5
and available in 100 sheet 8x10 packs. Brovira Speed is a cold tone paper.
Agfa also offers a Multicontrast RC product.
From Kodak, Polycontrast or Polymax are variable contrast papers.
Polycontrast is developer incorporated, as Kodabrome is, so it will process
in developers as well as many activators. Polymax requires the use of a
developer and will not process in activators. Ilford offers Multigrade IV
Deluxe which is not developer incorporated.
All of these papers use filters to control contrast, although a #5 filter
with any of them is not the same as a grade 5 Kodabrome. Both Polymax and
Multigrade IV have a slightly wider contrast range than Polycontrast.
For those who prefer a cooler tone print, Ilford also has a new paper,
Multigrade Cooltone, a non developer incorporated paper with a cooler image
tone and a cool white base tint.

George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com


} Just tried to order some Kodabrome II RC paper, grade F5. Vendor
} says it is discontinued. Any suggestions for an alternative? We have
} been using Kodabrome RC II for a long time with a Mohr processor. How
} about Polycontrast RC? Or is this the beginning of the end for old
} fashioned darkrooms?
}
} Jonathan Krupp



From daemon Thu Apr 12 09:57:54 2001



From: Randall, Kevin J :      Kevin.Randall-at-astrazeneca.com
Date: Thu, 12 Apr 2001 15:47:06 +0100
Subject: Thermanox and HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to process some thermanox coverslips for SEM. Does anyone know
whether thermanox survives HMDS?

Cheers

Kevin


From daemon Thu Apr 12 10:52:29 2001



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Thu, 12 Apr 2001 08:47:33 -0700
Subject: Re: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The paper is still listed on the Kodak Web sight, http://www.kodak.com/cluster/global/en/professional/support/techPubs/f33/old/f33.shtml . The web sight does list Kodabromide paper as discontinued but lists Kodabrome II RC as its replacement and does not list the latter as discontinued. I was curious, so, I called the 800 technical hotline (800) 242-2424 (option 02 gives you an operator) and asked. They said that it is still available but not in as many sizes as it use to be.

I've had venders tell me a product is discontinued when it is only discontinued from their stocking process, not by the manufacture. In this case it may be that only the size you wanted was discontinued. You may want to call the vendor back or check with another vender.

I have no connection with Kodak but wanted to see if the predictions I'd heard about, as you put it, " the beginning of the end for old fashioned
darkrooms," was starting. It would seem not quite yet in this case. Though I'm hearing that in 2 or 3 years it will, somewhat sad I think.

Jim Roberts


James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } {jmkrupp-at-cats.ucsc.edu} 04/11/01 03:25PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi:

Just tried to order some Kodabrome II RC paper, grade F5. Vendor says it is
discontinued. Any suggestions for an alternative? We have been using
Kodabrome RC II for a long time with a Mohr processor. How about
Polycontrast RC? Or is this the beginning of the end for old fashioned
darkrooms?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu








From daemon Thu Apr 12 11:11:12 2001



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Thu, 12 Apr 2001 12:05:47 -0400 (EDT)
Subject: Re: Thermanox and HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kevin,

Yes, Thermanox cover slips survive treatment with HMDS. We occationally
process cell monlayers grown on them for SEM.

Regards,

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Thu, 12 Apr 2001, Randall, Kevin J wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I need to process some thermanox coverslips for SEM. Does anyone know
} whether thermanox survives HMDS?
}
} Cheers
}
} Kevin
}



From daemon Thu Apr 12 12:09:32 2001



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Thu, 12 Apr 2001 12:15:33 -0500
Subject: TEM-SiC wafer-thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
Thank you all for sharing your experiences about working with SiC. Your
suggestions have helped me come up with a plan of attack.
Regards,
Mike Coviello
UT Arlington



From daemon Thu Apr 12 14:44:23 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Thu, 12 Apr 2001 13:06:26 -0400
Subject: Re: Kodabrome II RC discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jon Krupp,

We are a Kodak distributor and we checked with Kodak this morning and
Kodabrome II RC paper, grade F5 is still available. If you still
interested you may contact me off-line.

John Arnott

Jon Krupp wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Hi:
}
} Just tried to order some Kodabrome II RC paper, grade F5. Vendor says it is
} discontinued. Any suggestions for an alternative? We have been using
} Kodabrome RC II for a long time with a Mohr processor. How about
} Polycontrast RC? Or is this the beginning of the end for old fashioned
} darkrooms?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu



From daemon Thu Apr 12 16:50:03 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 12 Apr 2001 16:44:37 -0500
Subject: EM: LaB6 filaments in H7100 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are about to install a Kimball ES-423E (extended life) LaB6
cathode in a Hitachi H7100 TEM and were wondering if anyone had some
starting points for the voltage settings for filament heating. This
would save us a lot of time, if so.

Thank you.

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Apr 12 17:18:02 2001



From: Robert Fitton :      fittonro-at-luther.edu
Date: Thu, 12 Apr 2001 16:12:39 -0600
Subject: Ask-A-Microscopist:Help Cleaning Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is an odd way that I thought up to clean oil from an objective without
using solvents that may attack lens cement.

Remove the lens from the scope. I usually like to view the oil
contamination using a stereoscopic microscope.
Wipe excess oil away - I like using Ross Optical Paper.
Using a cotton applicator wrapped in Ross optical paper, apply a small
amount of Dawn dishwashing detergent. Gently work the surface to emulsify
the oil into the detergent using the optical papered applicator. You may
have to add a small amount of water. Do not allow fluids to go much beyond
the lens area!

At this point, hold the lens vertical with the back focal plane pointing
up. Apply a small amount of deionized water on the final lens element from
the side of the opjective to create a hanging drop. I usually use a wash
bottle or 10cc syringe. The surface tension of water will create a small
drop around the optical surface. Start a stream of DI water flowing
through the small drop to wash away the oil/water suspension. After about
a minute, stop the water flow while allowing a small drop of water to stay
on lens. At this time, blow the water off using C02 or some other clean
compressed gas. This will eliminate streaks. Upon inspection, if you see
contamination, repeat the process and your problem should be solved.

It's weird but it works.

Robert

Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

Voice 563-387-1559
FAX 563-387-1080

Enjoy a visit to our website: http://www.luther.edu/~biodept/




From daemon Thu Apr 12 22:39:25 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 12 Apr 2001 22:36:21 -0500
Subject: Re: Particle Concentration Determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Richard,
The most common use of this is for asbestos fibre load determination. I did
it once. You count the number of fibres in a specified number of grids
openings, then calculate back to the original collected volume or vacuumed
area. It should work similarly for any recognizable particle. I could look
up the method, if you like.
At 06:08 PM 4/11/01 -0400, you wrote:
}
} Has anyone had experience in determining the concentration of particles
} in a solution by counting on EM grids in a similar way to using a
} haemocytometer? Is it possible to count the particles in a defined
} number of grid squares then calculate back to the area of the grid and
} the amount of solution which was applied and allowed to dry down?
}
} Richard Gardiner

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


From daemon Thu Apr 12 22:39:53 2001



From: dngeorge-at-wam.umd.edu
Date: Thu, 12 Apr 2001 22:39:11 -0500
Subject: Ask-A-Microscopist:Question on Staining and wrinkles of sections.

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(dngeorge-at-wam.umd.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
April 12, 2001 at 16:04:35
---------------------------------------------------------------------------

Email: dngeorge-at-wam.umd.edu
Name: Damali Martin

Organization: University of Maryland

Education: Graduate College

Location: College Park, MD

Question: I have embedded and sectioned some salivary glands in epon
and have placed the sections on glass slides. However, when I
counterstain, a high percentage of sections float off and are lost.
How can this problem be prevented?

Also, several of my sections have wrinkles in them. Is there any
trick to getting rid of them?

Thank you.

---------------------------------------------------------------------------


From daemon Fri Apr 13 01:11:46 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 13 Apr 2001 18:12:04 GMT+1200
Subject: Stigmator image shift

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Hi

Anyone got any suggestions as to the cause of (and remedy for) the
pronounced image shift that occurs when I turn the Y stigmator
control on my JEOL 840A?

It also has the usual stigmatic effect.

The X control shifts the image only very little.

The coils seem to check out OK.

There's something quite poetic about working on this particular part
of the instrument today of all days.

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Apr 13 05:08:22 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 13 Apr 2001 05:03:36 -0500
Subject: RE: Stigmator image shift

Contents Retrieved from Microscopy Listserver Archives
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Stigmators operate as two opposing coils. It may be that one of the coils
for the Y stigmator is not active while the opposing Y coil is. Perhaps
one of the coils has shorted? The resistance of these coils is very low,
making a simple check rather difficult, not to mention that they are often
series connected within the column making physical access difficult. But
if you can measure the current drawn by each, you may find a significant
difference if one is shorted.

If so, the only remedy is replacement of the coils.

On Friday, April 13, 2001 1:12 PM, Ritchie Sims
[SMTP:r.sims-at-auckland.ac.nz] wrote:
}
} Hi
}
} Anyone got any suggestions as to the cause of (and remedy for) the
} pronounced image shift that occurs when I turn the Y stigmator
} control on my JEOL 840A?
}
} It also has the usual stigmatic effect.
}
} The X control shifts the image only very little.
}
} The coils seem to check out OK.
}
} There's something quite poetic about working on this particular part
} of the instrument today of all days.
}
} thanks
}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Fri Apr 13 08:08:13 2001



From: Gary Radice :      gradice-at-richmond.edu
Date: Fri, 13 Apr 2001 08:55:14 -0400
Subject: cleaning lenses

Contents Retrieved from Microscopy Listserver Archives
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About 25 years ago, when I was taking the embryology course at the
Marine Biology Laboratory at Woods Hole, we were taught the following
methods for cleaning oil from lenses by Robert Allen, a well known
microscopist:

Remove the lens from the microscope and invert it. Remove most of the
excess oil from the objective with lens paper without touching the
objective surface itself. Gently lay a strip of lens paper over the
objective. Since the lens is recessed the lens paper does not touch
the glass directly. Then place a drop or two of ether on the lens
paper just next to where it contacts the lens, and draw the lens
paper over the objective surface. The ether vapors swirl around under
the lens paper and dissolve the oil while the lens paper absorbs the
mixture and carries it away, without actually touching the glass
surface. You might need to repeat a few times to remove the oil.

It works with all but the most stubborn deposits. The upside is that
it completely avoids touching the glass surface, and the ether fumes
are in contact with the element such a short time that it miakes it
less likely you will dissolve the glues that hold the lens elements
together. The downside is that it requires ether. I haven't tried it
with other solvents.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice


From daemon Fri Apr 13 08:39:05 2001



From: Yurek, Peter :      Peter_Yurek-at-adc.com
Date: Fri, 13 Apr 2001 08:32:51 -0500
Subject: SEM Tech Position

Contents Retrieved from Microscopy Listserver Archives
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Please send resume to:

Sara M. Rohe
Technical Recruiter, BCG
ADC - The Broadband Company
Tel: 952.233.6474
Fax: 952.233.6652
sara_rohe-at-adc.com


ADC is a global lead in innovation broadband networks and applications. We redefine the economics, quality and benefits of broadband services that provide unlimited access to information anytime, anywhere.


To build and ensure quality production of Fiber products in line with department objectives. Pursue project objectives as assigned by Engineers. Assist in developing test systems and conduct testing of engineering samples.

Accountabilities % of time

70 Collect data from production activities to track and improve quality. Develop collection systems to optimize this process. We have a new Hitachi S3000N with an Oxford INCA EDS system and an Oxford/Gatan CL system with cryostage. Routine analysis will include EBIC, EDS, and CL.

20 Interface with Manufacturing Engineers, Application engineers, Detail Engineers, Product Managers, Technicians, Maintenance, Tool Room, Field Service, and other stakeholders to achieve assigned project goals. Recommend design changes to improve manufacturability and quality.

10 Coordinate project work activity, including testing, machining, and purchasing, as needed to ensure meeting project schedules. Design and build fixtures, prototypes, and samples. Provide training on equipment and techniques to assemblers.


Requirements:
Communication: Direct and concise. Keeps people informed. Listens effectively to others.
Teamwork: Effective at working in team situations.
Initiative/Results Orientation: Originates action. Finds ways to get things done.
Quality: Promotes continuous improvement. Effectively utilizes data.
Customer Responsiveness: Responds well to internal or external customers needs.
Education: Two-year telecommunications degree or equivalent training preferred.

Experience:
1 year of experience on SEM
Basic optical microscopy experience.
Routine handling of small parts.
Should be familiar with electrical test and basic electronics.
Soldering, ESD experience is a plus.

Please send resume to:

Sara M. Rohe
Technical Recruiter, BCG
ADC - The Broadband Company
Tel: 952.233.6474
Fax: 952.233.6652
sara_rohe-at-adc.com
Peter Yurek
Failure Analysis Engineer
ADC
Phone: 651-494-1441
Fax: 651-494-1470
Peter_Yurek-at-adc.com

Learn about ADC - The Broadband Company at www.adc.com

4459 White Bear Parkway
White Bear Lake, MN 55110




From daemon Fri Apr 13 09:23:11 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 13 Apr 2001 08:49:43 -0500
Subject: Re: Stigmator image shift

Contents Retrieved from Microscopy Listserver Archives
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I usually see image shift as a result of stigmation in both of our scopes
(JEOL 840A and Hitachi 2460N). I rather accepted that it just went with the
territory. If it is a sign of a problem with the scope or its alignment, I
too would be interested in hearing how to eliminate it.

Warren

At 06:12 PM 4/13/2001 +0000, you wrote:

} Hi
}
} Anyone got any suggestions as to the cause of (and remedy for) the
} pronounced image shift that occurs when I turn the Y stigmator
} control on my JEOL 840A?
}
} It also has the usual stigmatic effect.
}
} The X control shifts the image only very little.
}
} The coils seem to check out OK.
}
} There's something quite poetic about working on this particular part
} of the instrument today of all days.
}
} thanks
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Apr 13 09:23:14 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 13 Apr 2001 10:18:43 -0400
Subject: Re: Stigmator image shift

Contents Retrieved from Microscopy Listserver Archives
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Hi Ritchie,

The 840 has some pots (potentiometers) on one of the circuit boards that
adjust out
the image shift while adjusting the stigmators. They probably balance a
bias, or an
offset, in the circuit for the stigmation coils. If you have the manual
with the schematics,
they may be identified. I may be able to find it in ours, if you don't
have it.

Darrell Miles



From daemon Fri Apr 13 10:59:25 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 Apr 2001 08:57:58 -0700
Subject: Re: Stigmator image shift

Contents Retrieved from Microscopy Listserver Archives
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Normally, X or Y stig adjustment will cause image shifting.
More shifting at higher mag. Since stigmation is done with
coils by the scan coils, changing current in the stig coils
appears to the beam as a change in scan coil current. Hence,
the image shifts. Later day systems account for this by
feeding some of the stig signal to the scan coils as feedback.
It will also accept additional feedback from magnification.
The operation is to adjust scan coil current and condenser
current as stig is adjusted so that the net result is minimal
image shift with varying stig input.

Since one stig channel out of two is not working right, there
are likely one of two or three things which could go wrong.
First, and worst, the stig coil is open or shorted. Somewhat
unlikely I think. Second, one part of the stig coil drive circuit
has failed. Third, some part of the feedback system has
failed. Since you say that it does stig, but causes image
shift, then the stig circuit itself and the coil is OK. You can
verify this.

Since the stig coil drive systems are identical (ususally),
you should be able to trace readings from the good channel
and compare them to the bad channel. This should show
right away where the problem is. The stig, beam alignment
and image shift circuits are usually all the same. If so
in your system, they will give plenty of data points for checking.
The coils (one for X, one for Y) are typically driven by
push-pull power transistors which are high current buffers
on the output of a small op amp. The coils are in the negative feedback
loop of the op amp. One lead of a coil would connect to the
output of the buffer transistors (large ones) while the other end connects to
the inverting input of the op amp and is returned to ground
through a low value resistor. Measuring the voltage across this
resistor will tell how much current is flowing through the coil
(I=E/R).

Stig effect is lower at lower mags. So there would be less
automatic compensation for stig vs. mag. Try a low mag setting
and measure the voltage on each
side of the stig coils (two leads each) and the voltage on the
low value resistors. Have the stig controls at 12 O'clock each.
If these readings match, odds are that the coils are for sure OK. Then
the problem ought to be narrowed to the stig balance circuit. Look for this
circuit and compare the two stig signal feedback loops for
differences. It could be something as simple as a bad op amp
in the path from the stig circuit to the beam alignment coil
drivers. Since stig has little effect at low mag, but huge
effect at high mag, the usual control path for stig
compensaton versus magnification is to
electronically change the beam position by sending a stig
sourced voltage to the scan coil driver circuit.

If you don't have schematics for the system, that is of
course a major problem. In this case, perhaps someone
who has your same model has encountered this problem
before and knows of the failure mechanism and cause.

gary g.


At 11:12 AM 4/13/2001, you wrote:

} Hi
}
} Anyone got any suggestions as to the cause of (and remedy for) the
} pronounced image shift that occurs when I turn the Y stigmator
} control on my JEOL 840A?
}
} It also has the usual stigmatic effect.
}
} The X control shifts the image only very little.
}
} The coils seem to check out OK.
}
} There's something quite poetic about working on this particular part
} of the instrument today of all days.
}
} thanks
}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Fri Apr 13 11:21:36 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Fri, 13 Apr 2001 12:16:12 -0400
Subject: Stigmator image shift

Contents Retrieved from Microscopy Listserver Archives
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Hi Ritchie

Stigmator shift is almost always down to the balance of the stigmator
coils. Stigmators have 8 coils in four sets of two. If an opposing pair
are not correctly balanced the stronger coil will cause the image to move
away from that coil.

Some instruments have the balancing potentiometers easily accessible others
hide them away in the electronics.

The pots will be called Xx, Xy, and Yx, Yy I do not know where they are on
a JEOL 840 but if you look inside you may be lucky?

To adjust -
1. Place an easily recognizable feature in the center of the screen
2. Turn the X stigmator fully in one direction and re center with the
Xx or Xy control whichever fits.
3. Turn in the opposite direction and use the other compensation
control to center
4. Repeat with the Y stigmator and Yx and Yy controls.

I have just completed the promised "Monitoring & Maintaining Electron
Microscope Performance" interactive CD, this area is covered in the
instrument tuning section so its pretty fresh in my mind. Need to see even
more for yourself then we have the course of the same name running in
Sydney early October this year?

Kindest regards and good luck

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com



From daemon Fri Apr 13 12:28:42 2001



From: Christian Normand :      tekna-at-tekna.qc.ca
Date: Tue, 2 Feb 1999 13:58:53 -0700
Subject: Xray analyzer for Hitachi S-570 SEM

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

I have a Hitachi SEM model S-570 (1985). I am interested of getting a X-ray analyzer for this microscope. My preference would be to buy a used analyzer with an ultrathin window for light elements analysis.

If you know someone who is interested in selling it's analyzer, please let me know...

Thank you

Christian Normand
Tekna Systems Plasma
(819) 820-2204


From daemon Fri Apr 13 13:34:00 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 Apr 2001 11:34:40 -0700
Subject: Film Processing & dynamic range & scanners (longish)

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I hesitated to make any response regarding this subject.
But--here goes anyway. Someone may find it useful.

I think that the issue is not reduction of contrast but rather extension
of tonal range of the negative. That is what the zone system is
all about. I think that it is a fact that if the silver is not exposed,
there is not going to be any information gleaned from that
unexposed or too underexposed area. The approach I use for fine
art images is to overexpose and under-develop. It takes a LOT
of work to develop a complete system of ISO rating, development
and printing to achieve marketable prints. But it certainly can
be done. The goal is to minimize the time spent in the darkroom
doing printing. Ideally, the neg should print without any effort
on grade 3 paper. I only use Ilford archival matte fiber paper, so
extensions to RC papers may or may not directly apply. I also
do not use variable contrast paper. But the principles are extensible
I would think.

Being all-digital with my SEM, I use realtime histogram feedback
to adjust gain and dynamic range. For TEM, I have no direct
experience. But I might suggest that the same photo techniques
for fine art neg work may apply to TEM negs. If you think that this
might be true, read on. Otherwise, nevermind.


A neg with bad dynamic range (huge extremes of EV or low contrast) is not
going to produce a very good scan. This is the realm of drum
scanners with 4.0-6.0D. Pixel resolution is subordinate to
D range in this case. If one is going to print a neg on paper,
that is one aspect of the problem. If the neg is to be output
to a magazine or printed page, that is another aspect. So the
crux of the matter is what the actual intended end use of the
neg is to be? If it is a nice print, OK. If it is a magazine or litho
output, these are 133lpi. Doing scans at 4000dpi only to
print them in a magazine at 133lpi is rather silly. One would
probably be better off just printing the neg and scanning at
300dpi.

But the 300 dpi is the effective dpi for the size of
the final image....not the original neg. So, if the neg is say
3" x 4" and is to be printed at that same size, a 300 dpi scanner
should do the job (ignoring tonal range for the time being).
If the neg is to be printed at twice its physical size, then the
scanner has to have twice the resolution (600 dpi). And it
goes on from there. Thus, for most image output methods
on a printed page, 600-1200 optical dpi should be plenty of
resolution. If you agree with this discussion at this point,
then the resolution factor should no longer be an issue.
What remains is D range.

(Note that commercial images are created at high resolution
to accommodate manipulation and merging with other images.
This way, image quality can gradually be reduced without
affecting the final result.)

But the negative is the key item in all of this discussion. A
bad neg is not likely to do anyone much good. So the challenge
is to get a good neg at the get go. Here we go, back to the
zone system. The idea is to use the neg as a non-linear
image capture media for data which may span a wide
dynamic range. And in so doing, be able to output (print
or scan) this information with fidelity and minimal effort.
This drives D value.

An unexposed, developed neg will have a transmission of 100%.
It is actually slightly lower due to absorption by the medium,
emulsion and residual anti-dispersion coating. But at 100%
transmission, the neg would have an opacity of 100%input/100%output
or 1.0. Since D=log1/T, the D value for the unexposed neg
is log 1/1=0.0. This then is Dmin. The area with the most
exposure (darkest region) will pass the least amount of
transmitted light. If, for example, this region passes 1% of
the transmitted light, this is a Tval=100%/1%=100.
And D=log (1/100)=2.0. This then is Dmax. The D range
of the negative is Dmax-Dmin=2.0-0.0=2.0. So a scanner
with a D value of } 2.0 would capture the tonal range of this
neg. The tonal range of this neg is 100 tonal variations.
Prints generally have D values between 1.7 and 2.0.
So the same scanner would handle the neg and a typical
print.

Let's say that the darkest area of the neg transmits 0.1%
of the transmitted source light. This then yields Tval=100/0.1
=1000. And D=log 1000 = 3.0. Thus, this neg has 1,000
tonal variations in it from clear to black. If the neg has double
the number of tonal variations (2,000) that would mean
that the opacity is 2,000 and D=log 2000 = 3.3. At 4000
tonal range, D=3.6. Thus, each 0.3D equates to doubling
the opacity range or halving or doubling the transmission
value. Thus, if a scanner has a D rating of 3.0 (1000 tonal
ranges) and the neg passes a corresponding 0.1% of the
transmitted light, and another scanner has a D rating
of 3.3 (2000 tonal ranges) and the neg passes 0.05% of
the transmitted light--can you really tell one from the other?

The eye is more responsive to subtle changes in tonal
variations in the white region of an image than in the
dark ones. Thus, it is important to retain as much variability
and rendition in the darkest areas of the negs (the highlights)
but to do so without sacrificing detail in the shadows (clearer
areas of the neg). This is done using the zone system
and expansion and contraction of tonal range.

The response of the neg emulsion's exposure to light is not linear
over the range of clear to full black. Its response curve is somewhat
like a flattened S. The low exposure region is called the toe, which
consists of the film base + fog density (Dmin). Moving up the curve
is the straight line section (not exactly a straight line), and then to
the shoulder. At this point, increasing amounts of light do not
have corresponding quantitative amounts of change in density.
Further exposure reaches saturation, or Dmax. At this point,
no increase in exposure will change the density of the neg.

The ultimate goal is to maximize the straight line section and
move Dmax as high as possible, without detrimentally affecting
Dmin. I use contraction to accomplish this. The approach is
to overexpose and underdevelop. With a neg, the rule of thumb
is to expose for the shadows. If there is insufficient exposure
in the shadow areas, there will not be any detail rendered.

Using Ilford FP4+ film, I rate it at ISO 80 (mfg=125). Development
is done using stock developer diluted 1:1. It is used one time
and discarded. Never replenish or try to refresh the developer.
Development time will vary, depending on the tonal range of the
scene. Here are some extreme examples of how this system works:
(if you are offended by nude images, use the still life links. These
following links are to fine art nudes and still life which were done using the
zone system previously described)

[nudes]
A. This shot illustrates a typical impossible shot. Inside the room,
the exposure was about EV 3, the outside was dense fog with
a starch white picket fence at EV 11. A scene like this with
eight stops of variation would typically be shot to either render
detail in the highlights (fog and fence) or in the inside room's
details (shadows). To render shadow detail and retain highlight
detail, the zone system was used as described. The image
via this link is as-scanned on a UMAX Powerlook III of a
6x6cm negative using the transparency adapter. You can
see the detail in the paint on the wall and can see the fence in
the fog.
http://www.photoweb.net/pw_gal_nude/pw_gal_nude_1/g_2.html

B. This shot shows great shadow detail despite the outside light
creating a hot spot on the model's head. And the window
on the left was rendered, despite the high level of light.
http://www.photoweb.net/pw_gal_nude/pw_gal_nude_1/g_6.html

C. This shot shows a rather low contrast scene. There are no
remarkable highlights. There is much material in shadow.
Overexposure and ensuring at least two zones of exposure
for the shadows and then overdeveloping N+1 achieved
a perfectly printable neg. Again, this neg is shown as-scanned.
http://www.photoweb.net/pw_gal_nude/pw_gal_nude_6/g_3.html



[Still life]
A. high side lighting.
http://www.photoweb.net/pw_gal_still/pw_gal_still_4/g_5.html

B. Low shadow lighting, highlights present.
http://www.photoweb.net/pw_gal_still/pw_gal_still_4/g_6.html

C. Even lighting. There is actually more detail revealed
than is showed in this web pix.
http://www.photoweb.net/pw_gal_still/pw_gal_still_3/g_6.html



Non-web images of these negs are of course much better than
those on-line. But I hope these illustrate the points of the zone
system. I do think it can apply to TEM negs. The other point
is to keep in mind the relationship of D values of scanners to
what you are actually going to be scanning. If a scanner has
sufficient resolution, and say a D rating of 3.2. Are you
really going to be able to tell any difference using a scanner
with a 3.4 or 3.5D at much higher cost? If you have a
densitometer, check the D range of some of your negs. They
are probably all less than 3.0. Maybe I'm wrong in this
respect since I have little experience with TEM media.
But the idea is go get the equipment you need for the job
you need (the output destination and the use of the image)
based on the actual media being scanned. Otherwise, there
is a great opportunity to buy capability which will never be
utilized.

Despite all the discussion of processing negs, as more
digital capture and image processing products come out,
things will change. There are rather simple ways to expand
the contrast of an otherwise low contrast, poor neg. Likewise,
there are ways to extract subtle detail from negs which have
blown out highlights. More about this later.

gary g.


Reference:
Adams, A. (1981). The negative. Boston: Little, Brown and Company.
ISBN 0-8212-1131-5 (twelfth printing, 1992).



At 01:07 AM 4/12/2001, you wrote:

} Usualy to reduce contrast you under expose the film. Then develop it to
} the disired density using a developer that generates low contrast. One way
} to reduce contrast is to dilute you developer by a factor of 2, 4 or more
} with water. It extends the developing time a good deal but it reduces the
} contrast. You might also look at low contrast developers that work with
} the film you are using.
}
} A few question on rec.photo.darkroom will get you more information than
} you can handle and some of it will actually work.
}
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger



From daemon Fri Apr 13 14:30:59 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 13 Apr 2001 14:24:36 -0500
Subject: Re: Film Processing & dynamic range & scanners (longish)

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Good job, Gary! Gary's description of dynamic range is the clearest
and most easily understood I have seen (and I have been looking for a
good one!). I think I already understood most of what he said before
he said it but I was having a devil of a time trying to articulate
the concept to one of my staff. My only followup question is in
regards to his statement near the end where he suggests some test the
density of a TEM negative with a densitometer to see if they really
go much above 3.0. I would like to encourage any one who has or will
be measuring this for a typical and even finicky biological thin
section TEM image to please post the info on the Microscopy
listserver. Thanks.

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}
} I hesitated to make any response regarding this subject.
} But--here goes anyway. Someone may find it useful.
}
} I think that the issue is not reduction of contrast but rather extension
} of tonal range of the negative. That is what the zone system is
} all about. I think that it is a fact that if the silver is not exposed,
} there is not going to be any information gleaned from that
} unexposed or too underexposed area. The approach I use for fine
} art images is to overexpose and under-develop. It takes a LOT
} of work to develop a complete system of ISO rating, development
} and printing to achieve marketable prints. But it certainly can
} be done. The goal is to minimize the time spent in the darkroom
} doing printing. Ideally, the neg should print without any effort
} on grade 3 paper. I only use Ilford archival matte fiber paper, so
} extensions to RC papers may or may not directly apply. I also
} do not use variable contrast paper. But the principles are extensible
} I would think.
}
} Being all-digital with my SEM, I use realtime histogram feedback
} to adjust gain and dynamic range. For TEM, I have no direct
} experience. But I might suggest that the same photo techniques
} for fine art neg work may apply to TEM negs. If you think that this
} might be true, read on. Otherwise, nevermind.
}
}
} A neg with bad dynamic range (huge extremes of EV or low contrast) is not
} going to produce a very good scan. This is the realm of drum
} scanners with 4.0-6.0D. Pixel resolution is subordinate to
} D range in this case. If one is going to print a neg on paper,
} that is one aspect of the problem. If the neg is to be output
} to a magazine or printed page, that is another aspect. So the
} crux of the matter is what the actual intended end use of the
} neg is to be? If it is a nice print, OK. If it is a magazine or litho
} output, these are 133lpi. Doing scans at 4000dpi only to
} print them in a magazine at 133lpi is rather silly. One would
} probably be better off just printing the neg and scanning at
} 300dpi.
}
} But the 300 dpi is the effective dpi for the size of
} the final image....not the original neg. So, if the neg is say
} 3" x 4" and is to be printed at that same size, a 300 dpi scanner
} should do the job (ignoring tonal range for the time being).
} If the neg is to be printed at twice its physical size, then the
} scanner has to have twice the resolution (600 dpi). And it
} goes on from there. Thus, for most image output methods
} on a printed page, 600-1200 optical dpi should be plenty of
} resolution. If you agree with this discussion at this point,
} then the resolution factor should no longer be an issue.
} What remains is D range.
}
} (Note that commercial images are created at high resolution
} to accommodate manipulation and merging with other images.
} This way, image quality can gradually be reduced without
} affecting the final result.)
}
} But the negative is the key item in all of this discussion. A
} bad neg is not likely to do anyone much good. So the challenge
} is to get a good neg at the get go. Here we go, back to the
} zone system. The idea is to use the neg as a non-linear
} image capture media for data which may span a wide
} dynamic range. And in so doing, be able to output (print
} or scan) this information with fidelity and minimal effort.
} This drives D value.
}
} An unexposed, developed neg will have a transmission of 100%.
} It is actually slightly lower due to absorption by the medium,
} emulsion and residual anti-dispersion coating. But at 100%
} transmission, the neg would have an opacity of 100%input/100%output
} or 1.0. Since D=log1/T, the D value for the unexposed neg
} is log 1/1=0.0. This then is Dmin. The area with the most
} exposure (darkest region) will pass the least amount of
} transmitted light. If, for example, this region passes 1% of
} the transmitted light, this is a Tval=100%/1%=100.
} And D=log (1/100)=2.0. This then is Dmax. The D range
} of the negative is Dmax-Dmin=2.0-0.0=2.0. So a scanner
} with a D value of } 2.0 would capture the tonal range of this
} neg. The tonal range of this neg is 100 tonal variations.
} Prints generally have D values between 1.7 and 2.0.
} So the same scanner would handle the neg and a typical
} print.
}
} Let's say that the darkest area of the neg transmits 0.1%
} of the transmitted source light. This then yields Tval=100/0.1
} =1000. And D=log 1000 = 3.0. Thus, this neg has 1,000
} tonal variations in it from clear to black. If the neg has double
} the number of tonal variations (2,000) that would mean
} that the opacity is 2,000 and D=log 2000 = 3.3. At 4000
} tonal range, D=3.6. Thus, each 0.3D equates to doubling
} the opacity range or halving or doubling the transmission
} value. Thus, if a scanner has a D rating of 3.0 (1000 tonal
} ranges) and the neg passes a corresponding 0.1% of the
} transmitted light, and another scanner has a D rating
} of 3.3 (2000 tonal ranges) and the neg passes 0.05% of
} the transmitted light--can you really tell one from the other?
}
} The eye is more responsive to subtle changes in tonal
} variations in the white region of an image than in the
} dark ones. Thus, it is important to retain as much variability
} and rendition in the darkest areas of the negs (the highlights)
} but to do so without sacrificing detail in the shadows (clearer
} areas of the neg). This is done using the zone system
} and expansion and contraction of tonal range.
}
} The response of the neg emulsion's exposure to light is not linear
} over the range of clear to full black. Its response curve is somewhat
} like a flattened S. The low exposure region is called the toe, which
} consists of the film base + fog density (Dmin). Moving up the curve
} is the straight line section (not exactly a straight line), and then to
} the shoulder. At this point, increasing amounts of light do not
} have corresponding quantitative amounts of change in density.
} Further exposure reaches saturation, or Dmax. At this point,
} no increase in exposure will change the density of the neg.
}
} The ultimate goal is to maximize the straight line section and
} move Dmax as high as possible, without detrimentally affecting
} Dmin. I use contraction to accomplish this. The approach is
} to overexpose and underdevelop. With a neg, the rule of thumb
} is to expose for the shadows. If there is insufficient exposure
} in the shadow areas, there will not be any detail rendered.
}
} Using Ilford FP4+ film, I rate it at ISO 80 (mfg=125). Development
} is done using stock developer diluted 1:1. It is used one time
} and discarded. Never replenish or try to refresh the developer.
} Development time will vary, depending on the tonal range of the
} scene. Here are some extreme examples of how this system works:
} (if you are offended by nude images, use the still life links. These
} following links are to fine art nudes and still life which were done using the
} zone system previously described)
}
} [nudes]
} A. This shot illustrates a typical impossible shot. Inside the room,
} the exposure was about EV 3, the outside was dense fog with
} a starch white picket fence at EV 11. A scene like this with
} eight stops of variation would typically be shot to either render
} detail in the highlights (fog and fence) or in the inside room's
} details (shadows). To render shadow detail and retain highlight
} detail, the zone system was used as described. The image
} via this link is as-scanned on a UMAX Powerlook III of a
} 6x6cm negative using the transparency adapter. You can
} see the detail in the paint on the wall and can see the fence in
} the fog.
} http://www.photoweb.net/pw_gal_nude/pw_gal_nude_1/g_2.html
}
} B. This shot shows great shadow detail despite the outside light
} creating a hot spot on the model's head. And the window
} on the left was rendered, despite the high level of light.
} http://www.photoweb.net/pw_gal_nude/pw_gal_nude_1/g_6.html
}
} C. This shot shows a rather low contrast scene. There are no
} remarkable highlights. There is much material in shadow.
} Overexposure and ensuring at least two zones of exposure
} for the shadows and then overdeveloping N+1 achieved
} a perfectly printable neg. Again, this neg is shown as-scanned.
} http://www.photoweb.net/pw_gal_nude/pw_gal_nude_6/g_3.html
}
}
}
} [Still life]
} A. high side lighting.
} http://www.photoweb.net/pw_gal_still/pw_gal_still_4/g_5.html
}
} B. Low shadow lighting, highlights present.
} http://www.photoweb.net/pw_gal_still/pw_gal_still_4/g_6.html
}
} C. Even lighting. There is actually more detail revealed
} than is showed in this web pix.
} http://www.photoweb.net/pw_gal_still/pw_gal_still_3/g_6.html
}
}
}
} Non-web images of these negs are of course much better than
} those on-line. But I hope these illustrate the points of the zone
} system. I do think it can apply to TEM negs. The other point
} is to keep in mind the relationship of D values of scanners to
} what you are actually going to be scanning. If a scanner has
} sufficient resolution, and say a D rating of 3.2. Are you
} really going to be able to tell any difference using a scanner
} with a 3.4 or 3.5D at much higher cost? If you have a
} densitometer, check the D range of some of your negs. They
} are probably all less than 3.0. Maybe I'm wrong in this
} respect since I have little experience with TEM media.
} But the idea is go get the equipment you need for the job
} you need (the output destination and the use of the image)
} based on the actual media being scanned. Otherwise, there
} is a great opportunity to buy capability which will never be
} utilized.
}
} Despite all the discussion of processing negs, as more
} digital capture and image processing products come out,
} things will change. There are rather simple ways to expand
} the contrast of an otherwise low contrast, poor neg. Likewise,
} there are ways to extract subtle detail from negs which have
} blown out highlights. More about this later.
}
} gary g.
}
}
} Reference:
} Adams, A. (1981). The negative. Boston: Little, Brown and Company.
} ISBN 0-8212-1131-5 (twelfth printing, 1992).
}
}
}
} At 01:07 AM 4/12/2001, you wrote:
}
} } Usualy to reduce contrast you under expose the film. Then develop it to
} } the disired density using a developer that generates low contrast. One way
} } to reduce contrast is to dilute you developer by a factor of 2, 4 or more
} } with water. It extends the developing time a good deal but it reduces the
} } contrast. You might also look at low contrast developers that work with
} } the film you are using.
} }
} } A few question on rec.photo.darkroom will get you more information than
} } you can handle and some of it will actually work.
} }
} } Gordon
} } Gordon Couger gcouger-at-couger.com
} } Stillwater, OK www.couger.com/gcouger

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Apr 13 14:33:16 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 13 Apr 2001 15:29:48 -0400
Subject: Re: Stigmator image shift

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Hi Ritchie,

I tried sending this earlier, but seems to have not made it.

The 840 has some pots (potentiometers) on one of the circuit boards
that are used to adjust out any image shift during stigmation adjustments.
They probably adjust some bias voltages, or some offsets, in the
stigmator circuits. They can be adjusted so there is no image shift.

If the pots can't eliminate the image shift, then a component has probably
gone bad, rather than just drifted a bit. If you have the manual with the
schematics, they should be identified in there. If you don't have the
manual, I might be able to find them in ours. Let me know.

Darrell Miles



From daemon Fri Apr 13 15:13:25 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Fri, 13 Apr 2001 16:12:26 -0400
Subject: New England Society for Microscopy (NESM) Woods Hole Meeting May

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The 18th Annual NESM Woods Hole Symposium will be held May 11-12th,
at the Marine Biological Lab at Woods Hole, MA.

This meeting is supported by members of the Connecticut Microscopy
Society (CMS), the Metropolitan Microscopy Society (MMS), and the New
York Society of
Experimental Microscopists (NYSEM).

Pre-registration is encouraged and is a must if you plan to attend
the Friday night dinner. Inquiries re: registration for this
meeting should be directed to Mary McCann (617) 484-7865 or by email:
mccanns-at-tiacc.net. Advance regis- tration including dinner on May
11th is $40.00 for NESM members, and $55.00 for non-members (this
cost includes a one- year membership to NESM).

PLEASE NOTE: ADVANCE REGISTRATION MUST BE RECEIVED no later than MAY 4th!!!
Registrations received after May 4th will NOT include dinner.

Friday, May 11th begins at Noon and consists of 2 sessions with an afternoon
coffee break. Following the presentations, a cocktail hours and
dinner will commence in the Swope Center.

Saturday, May 12th, NESM will present a symposium on Remote Access Microscopy.
Afterwards, there will be commercial exhibits and posters on display in the
Swope Center. Presentation of Poster and Photos-As-Art Awards and
Door Prizes will follow. This year lunch at the Swope Center will be
optional; those interested will pay $16.00. After lunch, two
45-minute tours of the Marine
Resource Center will take place.

NESM welcomes new members to the Society! Please join us for this
most enjoyable meeting.

Peggy Sherwood, Corresponding Secretary
NESM
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Fri Apr 13 15:57:20 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 Apr 2001 13:58:55 -0700
Subject: RE: Film Processing & dynamic range & scanners (shorter)

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Bernie:

Thanks for the reply.

You have a really good point for discussion about actual available exposure
time. Real world nudes and still lifes can be for several seconds exposure.
What is it for a typical TEM neg? I don't know. I suspect the same drift
problems occur in TEM as they do in SEM. So I too like to get the shot
as quickly as possible yet with minimum noise. So it is a tradeoff of
pixel density and pixel dwell time. Some original loss can be made up
later using the computer. With a digital active scan and image capture
system synchronized to 60 Hertz, a SEM shot at 3000x3200 pixels
takes 96 seconds at 10uS dwell time. This works fine. But for a TEM
which may not have sync to line frequency, I can see that image
shift is a big problem. Are TEM cameras and scanning routinely
synchronized to line frequency?

I used Diafine and Accufine back in photojournalism days to push
process TriX to ISO1600 or beyond. The job was to shoot basketball and
football
games without strobe. The reason was to gain even lighting and high
contrast shots. Since the pix were physically small, the higher grain
was not a big issue.

Your Diafine approach to TEM negs sounds like a solid method. Indeed,
the key is to get the shot and make a print with minimum amount of
tweaking (burning, dodging, etc.). If one were to only have to make
one print ever from one neg, it would not matter all that much. But
if more than one print is or will be made, reproducibility is a tough
issue with a less than perfect neg.

I see a similarity in your approach and what I was talking about. Your
procedure limits the development time of the neg. But while it limits the
time for dense areas, it limits the time for the whole neg too. The
procedure I described ensures that as much light information is
captured on the film, but extends the tonal range of the film by
reducing the strength of the developer and the development time.

I already have de-rated the speed of the film in this procedure. It may
be that doing this with TEM negs makes the resulting absolute speed
too slow. What is the rated ISO speed of some TEM media in use today?

gary g.



At 01:56 PM 4/13/2001, you wrote:
} Reply to: RE: Film Processing & dynamic range & scanners (longish)
} I saved your extensive info about negs. I would not use Diafine for
} regular scenic photos--too low in contrast. However, the manner in which
} split developers achieve this is to LIMIT the amount of developer
} available to "dense" negative areas. I only use it for contrasty TEM
} negs. that I routinely print on #2 or #3 paper , whichever is more
} pleasing. By having easily retrievable info in all of a negative, less
} time should be needed by any final printing method. The developer has a
} long tank life and works at room temperature. An extreme test of TEM negs
} made at 4 f/stops equivalent underexposure still printed nicely on #3
} grade paper.
} Of course I used a very long 12 mimutes in each half of the split
} developer, but this can allow short exposure on specimens with a
} "drifting" problem and save an expensive experiment.
} As primarily an old "wet" printer, I enjoyed your thorough
} explaination and have heard of the zone system and the great A. Adams. I
} like to get good results with little effort, thats all. If you ever want
} a copy of the Diafine Co.'s explaination of it's product, I can send you
} one. Nearly all of my published photos in Ultramicroscopy were done with
} this stuff, including a "lucky" cover photo on Ultra. 25 (1988) 351-354.
}
} Bernie Kestel E-mail: {kestel-at-anl.gov}
} Materials Science Division
} Argonne National Lab
} 9700 So. Cass Ave.,
} Argonne Il., 60439
} Gary Gaugler wrote:



From daemon Fri Apr 13 16:46:03 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 13 Apr 2001 17:41:53 -0400
Subject: Re: Particle Concentration Determination

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Has anyone had experience in determining the concentration of particles
in a solution by counting on EM grids in a similar way to using a
haemocytometer? Is it possible to count the particles in a defined
number of grid squares then calculate back to the area of the grid and
the amount of solution which was applied and allowed to dry down?

Dear Richard,
I can forsee one difficulty. The particles may not be deposited uniformly
due to a number of factors: 1) Evaporation of the applied drop of specimen
will not be uniform, so the particles could be dragged in toward the grid center
as the edges evaporate (or they could be preferrentially deposited toward the
edges if they are hydrophobic). 2) The grid surface may not be flat, causing
particles to deposit preferrentially in the centers of the grid squares--if
these are lower--or near the grid bars. 3) The particles could be
preferrentially deposited either on top of the grid bars or on the open areas,
which could look like uniform deposition, but would give erronious quantitation.
Of course, one could test this by depositing a known amount of suspension of a
known concentration of the kind of particles to be measured, then seeing if the
grid was covered uniformly and if the calculation gave the correct result. Good
luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Sat Apr 14 08:46:49 2001



From: McKayLodge-at-aol.com
Date: Sat, 14 Apr 2001 08:38:30 -0500
Subject: Agents for objective lens cleaning: Summary

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Dear ASK-A-MICROSCOPIST participants.

I asked if xylene was the best solvent for cleaning immersion oil off a
non-immersion objective. I recalled in my brief training that xylene was
safe but alcohols dissolve the more common lens adhesives (I could be wrong
and have this reversed). Here is the array of responses I received. The
diversity may interest you. THANKS to all for your advice.

1
We regularly use xylene with a cotton tipped applicator to clean our lenses
and remove immersion oil. This was recommended to us by the supplier of our
lenses and microscopes for a number of reasons. The primary reason is that
xylene effectively 'cuts' the oil, without damaging the lens. Acetone will
affect the lens coating, and Isopropyl alcohol only rinses the oil without
effectively removing it. Hope this helps

2
You are lucky that the lens elements did not dislodge. Use alcohol on lens
paper. Never use xylene or toluene; these solvents can dissolve the cement
used to seat the various lens elements.

3
I would recommend Sparkle Glass Cleaner. I only use solvents as a very
last resort (unless you really, really do want to upgrade) The cements are
soluble in most solvents. With intractable grime, I use a cotton tipped
applicator moistened with either xylenes or toluene and shake all excess
off. The tip must be moist, not wet or dry and make single passes until
the grime come off. Only enough solvent on the applicator to dampen the
surface, not enough to wet.

4
For day to day cleaning of lenses, including oil on the 40X. (I can't
believe this is the first time! The graduate students and Post Doc's drag
the40X through the oil at least twice a week. I have given them repeated
instructions but they seem intractable) Invert an ocular and examine the
surface for cleanliness. If it is clean, don't clean it. Dust off any
loose debris. Check the lens again. Moisten a cotton tipped applicator in
Sparkle and wipe the lens 1 swipe with a rolling action to present a new
surface and lift off any grit. Discard. Take a dry applicator and remove
the film. Check the lens with an inverted ocular to see if it is clean. Do
no more than is necessary. There has been an ongoing rampage on the list
about lens cleaning.... to solvent, not to solvent... lens tissue, not to
lens tissue etc.. I can
append you a couple, and a microscope maintenance handout if you would
like. It will be a bit long about 15 pages (38K).

5
The care instructions for my immersion oil suggest cleaning with a soft cloth
or lens tissue (no Kimwipes) moistened with ether/alcohol (7:3) or xylenes.
My microscope
manuals recommend removing finger prints using alcohol. Sounds like you're
good to go.

6
We use 70 % isopropanol to clean emersion oil off of our lenses.

7
Cleaning with xylene is a little drastic. If the lens eventually gets
xylene in behind the cement it could do some damage and you would have to
send it in to Nikon for repair. I use Kodak lens cleaner and some lens
cleaning tissues (not regular Kim wipes) to get the oil off, constantly
checking them under a dissecting scope to make sure that it is all removed.
It works well on some expensive lens that we have here.

8
We use Green Soap from the pharmacy. It works the best with ultra pure water.

9
Actually, you've got your solvents
reversed; alcohol is usually safer. But safer still is diluted detergent
"Joy" or similar, followed by water. Use alcohol only if that doesn't
work. And fumes from xylene, toluene, etc. are best avoided.

10
Back in the pre PC (chemical) days, toluene was a recommended
} solvent for cleaning lenses of immersion oil. Now, we at National Steel,
} wipe off excess oil with lens tissue and then clean the lens with Kodak lens
} cleaner solution.


From daemon Sat Apr 14 08:49:10 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 15 Apr 2001 09:30:04 GMT+1200
Subject: Stig Image Shift thanks

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Ritchie,
What you are describing is a stigmator drive that is out of calibration.
If you look on the schematic for the stigmator drive, you should see the
X(or Y) control that goes to an op-amp that in turn feeds a voltage divider
which in turn controls a different coil in the stigmator coil assembly.
Each part of this voltage divider has its own trim pot for calibration
purposes. Simply rock the X stigmator control back and forth while
adjusting each pot for minimum image shift. Then, increase your mag &
repeat. You should be able to get all of the shift completely out. Good
luck!

Gary M. Easton, Pres.
Scanners Corporation
SEM/EDS/IMAGING Sales & Service

----- Original Message -----
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, April 13, 2001 2:12 PM



Thanks very much to all those who responded to my post.

My hands didn't bleed at all.

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Apr 15 05:57:39 2001



From: pjpdo-at-kimo.com.tw
Date: Sun, 15 Apr 2001 01:50:17 -0800
Subject: FYI

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ery ten victims are male! {BR}
{BR}
{/FONT}
{FONT color=3D"#800040"} How the Snore Eliminator will help you! {BR}
{BR}
{/FONT}
{FONT color=3D"#800040"} {B} What Causes Snoring? {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
Over-relaxed throat and tongue muscles due to poor throat muscle {BR}
Excessive fat on the throat {BR}
Accumulation of secretion in the back of the throat and {BR}
Throat swelling from allergies and food {BR}
{BR}
Heavy snoring is often associated with apnea, or the {/FONT}
{FONT color=3D"#800040"} stopping of breathing, {/FONT}
{FONT color=3D"#0000A0"} during sleep. As an individuals sleeps his or her=
sleeping pattern is disrupted and fragmented due to snoring, thus they ar=
e never well rested. This can be a very hazardous problem if someone is no=
t getting proper REM sleep. Snoring is the audible symptom of a {/FONT}
{FONT color=3D"#0000A0"} {B} blocked airway during sleep. {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
The noise is caused by a vibration in the soft palate as the lungs pull ha=
rd to take in a {/FONT}
{FONT color=3D"#0000A0"} {B} weakened current of incoming air. {/B} {/FONT}
{FONT color=3D"#0000A0"} This blockage may result from any number of circu=
mstances, and these offer clues to get rid of the problem. {BR}
{BR}
Snoring is also more than likely to occur among people who {/FONT}
{FONT color=3D"#0000A0"} {B} sleep on their backs {/B} {/FONT}
{FONT color=3D"#0000A0"} ; the tongue falls back toward the throat and par=
tly closes the airway. {BR}
{BR}
{/FONT}
{FONT color=3D"#800040"} {B} The hidden dangers of snoring? {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {B} {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {UL} {LI} Snoring could increase the chances of a h=
eart attack if not treated {BR}
{LI} Hypertension and increased anxiety are associated with snoring {BR}
{LI} Snoring causes sleep apnea a serious treatable medical condition {BR}
{/LI} {/LI} {/LI} {/UL} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#800040"} {B} Snoring decreases sexual performance {/B} {/FONT=
}
{FONT color=3D"#800040"} {/FONT}
{FONT color=3D"#0000A0"} by half, due to lack of oxygen to the brain which=
decreases sexual responsiveness. {BR}
{BR}
Most people who snore have a {/FONT}
{FONT color=3D"#800040"} {B} shortage of oxygen {/B} {/FONT}
{FONT color=3D"#0000A0"} for a significant portion of that person's life.=
In recent studies, daytime fatigue and cardiovascular disorder are relate=
d to snoring. In a few cases, heavy snoring is a sign of potentially life =
-threatening problem. Sleep apnea in which breathing stops for a second or=
even minutes at a time and finally resumes with snorting and tossing arou=
nd. This pattern may be repeated hundreds of times all night long. The res=
ult is chronic oxygen shortage, which leads to abnormal heart rhythms, hig=
h blood pressure, and heart strain. {BR}
{BR}
{/FONT}
{FONT color=3D"#800040"} {B} How the Snore Eliminator works! {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} Snore Eliminator offers a unique break through wi=
th its all {/FONT}
{FONT color=3D"#800040"} natural enzymes {/FONT}
{FONT color=3D"#0000A0"} which metabolize the secretions, allowing the bo=
dy to absorb them into the back of the throat. The herbs reduce tissue swe=
lling. The result is to open the airway and smooth the airflow. This in tu=
rn eliminates the snoring for hours at a time. It also {/FONT}
{FONT color=3D"#800040"} {B} reduces the noise {/B} {/FONT}
{FONT color=3D"#800040"} made by breathing through the mouth while sleepi=
ng {/FONT}
{FONT color=3D"#0000A0"} . By simply spraying the back of the throat, tong=
ue and uvula with our special formula, manufactured using our patented bre=
ak through process, the soft tissue is coated. This allows the {/FONT}
{FONT color=3D"#0000A0"} {B} {/B} {/FONT}
{FONT color=3D"#800040"} {B} throat a chance to relax {/B} {/FONT}
{FONT color=3D"#0000A0"} thus a reduced amount of snoring. In most cases =
snoring is eliminated. Snore Eliminator coats the throat area with a uniqu=
e patented process of all natural lubricants. {BR}
{BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} TESTIMONIALS from several of our satisfied cus=
tomers: {BR}
{BR}
{/B} {/FONT}
{FONT face=3D"Times New Roman"}
{FONT color=3D"#FF0000"} {B} Lorenzo writes... {BR}
{BR}
I am having a good nights sleep, finally. I used to wake myself up during=
the night which was a real bummer as I would not always go back to sleep.=
Sleeping through the night is great. Thanks a lot Snore Eliminator. {BR=
}
PS: My girlfriend loves it as much or more than I do. {BR}
{BR}
Alex writes... {BR}
{BR}
My husband uses it for another reason. He has a problem with blocked nasa=
l passages. While he's asleep the passages close, he stops breathing and =
he wakes up gasping. When he takes Snore Eliminator his nose stays cleare=
r and he's able to sleep more restfully. {BR}
Thank you for a product that has really helped us! {BR}
{BR}
Glen writes... {BR}
{BR}
In February 1995, I was diagnosed with severe sleep apnea. A CPAP was the=
suggested treatment for my affliction, but it did not help my condition. =
I heard your advertisement and ordered a bottle of Snore Eliminator and m=
y sleep improved immediately. I continue to use it and my sleep has been =
better than it had been in years. Thanks. {BR}
{BR}
Steve writes... {BR}
{BR}
Let me take this opportunity to thank you for your wonderful product and t=
ell you how much my wife, Tracy, and I appreciate the quiet nights we have=
enjoyed since discovering it. It's very rare when a product actually del=
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of the other advertised remedies without any satisfaction and I am very p=
leased to be able to write and say that this product is as close as you ca=
n get to an absolute "Miracle". {BR}
{BR}
{/B} {/FONT}
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#800040"} If you have a snoring problem what can you do? {/F=
ONT}
{FONT color=3D"#0000A0"} Who would you turn to? Look no further, the ans=
wer to your snoring problems are over. Let our patented {/FONT}
{FONT color=3D"#0000A0"} {B} Snoring Eliminator {/B} {/FONT}
{FONT color=3D"#0000A0"} help you get through those sleepless nights. We =
guarantee it. In fact, we are so confident in Snore Eliminator that if you=
are not 100% satisfied with it you can return the unused portion for a fu=
ll refund, no questions asked* {BR}
{BR}
It is estimated that 90 million Americans, over the age of 18,suffer from =
habitual snoring. {BR}
{BR}
{/FONT}
{FONT color=3D"#800000"} {B} How to Use Snore Eliminator: {/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} Open your mouth {BR}
Hold the 4oz bottle about four inches from your open mouth {BR}
Spray one or two application onto the back of the throat. {/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{BR}
Snore Eliminator is safe to use nightly because of its' natural ingredient=
s. {BR}
Please note as with any health-related product, it is recommended that you=
consult with your physician prior to use if you have any medical conditio=
ns. {BR}
{BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} A Money Back Guarantee! * {BR}
{/B} {/FONT}
{FONT face=3D"Times New Roman"} {BR}
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#0000A0"} {B} We are so confident in Snore Eliminator that w=
e offer a 15-day money back guarantee. Merely return the unused portion o=
f Snore Eliminator within 15 days and we will refund your purchase price w=
ith no questions asked! {BR}
{BR}
Our normal price for Snore Eliminator is $29.95, but for a limited time on=
ly, you can purchase it for only {/B} {/FONT}
{FONT color=3D"#800040"} {B} $19.49 plus $3.95 {/B} {/FONT}
{FONT color=3D"#0000A0"} {B} for shipping handling. We will also continue t=
o offer you Snore Eliminator at this low price as long as you purchase fro=
m us in the future. To take advantage of this savings you must order with=
in the next 10 days. {BR}
{/B} {/FONT}
{FONT face=3D"Times New Roman"} For Shipping OUTSIDE the US please add $11=
.00.
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#0000A0"} {B} {BR}
{BR}
Here's How to order {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
You can order by mailing us a Check, Cash or a Money Order. You can also =
order by faxing us a copy of your Check. Faxing your order to us speeds y=
our order up by 3-5 days. {BR}
{BR}
To order by Check, Cash or Money Order merely send $19.49 plus $3.95 for a=
total of $23.44 per bottle. 1 bottle of Snore Eliminator will last you 4-=
6 weeks. Order TWO bottles for $19.49 and we do not charge you any shippin=
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re Eliminator for only $38.98, including shipping and handling. This is a =
17% savings. {BR}
{/FONT}
{FONT face=3D"Times New Roman"} For Shipping OUTSIDE the US please add $11=
.00.
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#0000A0"} {BR}
{BR}
Mail your check, cash or Money Order along with your name address, phone n=
umber and email address filled out on the following form: {BR}
{BR}
Your Name:___________________________________________(please print) {BR}
{BR}
Street: ______________________________________________ {BR}
{BR}
City:______________________State/Province:____________________ {BR}
{BR}
Zip/Postal Code:_________________Country:_________________________ {BR}
{BR}
Phone number:______________________________________________ {BR}
{BR}
FaxNumber:_________________________________________________ {BR}
{BR}
Email address:_______________________________________ {BR}
(if there is a problem with your order this is where we will notify you) {B=
R}
{BR}
To: {BR}
{BR}
John Taylor {BR}
CEO {BR}
Internet Information Services, Snore Eliminator Division {BR}
PO Box 21442 {BR}
Billings, MT 59104 {BR}
{BR}
{BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} {BR}
To Fax us your Order (Fill out and fax us every page which follows): {BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} {BR}
{/FONT}
{FONT color=3D"#0000A0"} {B} MAKE YOUR CHECK PAYABLE TO {/B} {/FONT}
{FONT color=3D"#0000A0"} : Internet Information Services, Snore Eliminator=
Division, tape your check to the following form and Fax to {/FONT}
{FONT color=3D"#0000A0"} {B} 1-775-640-3120 {/B} {/FONT}
{FONT color=3D"#0000A0"} . If this line is busy, please try faxing to {BR=
}
{/FONT}
{FONT color=3D"#0000A0"} {B} 1-208-692-5321. {/B} {/FONT}
{FONT color=3D"#0000A0"} Faxing your check to us speeds your delivery up =
by 3-5 days. {BR}
{BR}
Please ship me 1 bottle of Snore Eliminator for $19.49 plus $3.95 shipping=
and handling for a total of $23.44 per bottle. Order TWO bottles for $19=
.49 and we do not charge you any shipping and handling. This means you wi=
ll receive an 8 to 12 week supply of Snore Eliminator for only $38.98, inc=
luding shipping and handling. This is a 17% savings. {/FONT}
{FONT face=3D"Times New Roman"}
{FONT color=3D"#0000A0"} {BR}
{/FONT} For Shipping OUTSIDE the US please add $11.00. {BR}
{FONT face=3D"MS Sans Serif"}
{FONT color=3D"#0000A0"} {BR}
Your Name:___________________________________________(please print) {BR}
{BR}
Street: ______________________________________________ {BR}
{BR}
City:______________________State/Province:____________________ {BR}
{BR}
Zip Code:_________________Country:_________________________ {BR}
{BR}
Phone number:______________________________________________ {BR}
{BR}
Fax Number:_________________________________________________ {BR}
{BR}
Email address:_______________________________________ {BR}
(if there is a problem with your order this is where we will notify you) {B=
R}
{BR}
************************************************************** {BR}
{BR}
Internet Information Services Check Authorization and Order Form {BR}
{BR}
I wish to authorize the purchase of the Snore Eliminator from Internet Inf=
ormation Services using this order and check authorization form. I hereby=
authorize Internet Information Services to duplicate the attached check i=
n bank draft form for the amount shown on this attached check. I will ret=
ain my original copy for my record of this transaction. {BR}
{BR}
This Authorization is valid for this transaction only. No other bank draf=
ts may be created without my direct written authorization. {BR}
{BR}
Dated:_________________________________ {BR}
{BR}
Signed:________________________________________ {BR}
{BR}
{BR}
.Tape your check here and Fax it to us... {BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
{BR}
Fax this form to: {/FONT}
{FONT color=3D"#0000A0"} {B} 1-775-640-3120 {/B} {/FONT}
{FONT color=3D"#0000A0"} . If this line is busy, please try faxing to {/FO=
NT}
{FONT color=3D"#0000A0"} {B} 1-208-692-5321. {BR}
{BR}
{/B} {/FONT}
{FONT color=3D"#0000A0"} Thank you for your business, {BR}
{BR}
John Taylor {BR}
CEO {BR}
Internet Info Service, Snore Eliminator Division {BR}
PO Box 21442 {BR}
Billings, MT 59104 {BR}
{BR}
{BR}
{BR}
{BR}
{BR}
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From daemon Sun Apr 15 15:12:56 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Sun, 15 Apr 2001 16:03:56 -0400
Subject: Re: TEM-SiC wafer sample prep?

Contents Retrieved from Microscopy Listserver Archives
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The FIB works for SiC!

Regards,
Lucille Giannuzzi

At 12:53 PM -0400 4/10/01, Ronald Anderson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Mon Apr 16 07:05:17 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 16 Apr 2001 09:16:38 -0400
Subject: Fwd: TEM

Contents Retrieved from Microscopy Listserver Archives
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Richard

I am sending this message again because it was blocked by a list server
filter - presumably because 'home' is part of your e-mail address.

Malcolm

-------- Original Message --------


Please reply directly to:

} From: {hmskaug-at-tartarus.uwa.edu.au}
}
} Subject: TEM
}
}
} Hi,
}
} I am doing some preperation work for an assignment, and have a question.
}
} If a fresh unfixed piece of brain tissue (weighing approx. 1 gram) is
} received in a diagnostic electron microscopy laboratory, how would I
} proceed with the specimen? The brain is taken in surgery within the last 5
} min.
}
} If I was asked to carry out a rapid viral diagnosis on the tissue, what
} precautions should I take, and how would I proceed?
} I read that prions are able to survive fixation. What then?
}
} A respond is very much appreciated.
}
} Thank You !
}
} Sincerely,
}
} Hege Skaug

Greg Erdos
Assistant Director
Biotechnology Program Ph. 352-392-1295
University of Florida Fax 352-846-0251
PO Box 118525
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Mon Apr 16 11:55:25 2001



From: Alan Fox :      fox-at-nps.navy.mil
Date: Mon, 16 Apr 2001 09:49:43 -0700
Subject: Standard sample for EDS quant

Contents Retrieved from Microscopy Listserver Archives
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To all colleagues who supply standards for EDS work
I need a standard sample of known thickness to check the
accuracy of my TEM/EDS system during standardless quant. I need to
include light elements (Z {11) as I have a Moxtek SUTW window on my
detector. I guess an amorphous glass sample that contains both nitrogen
and oxygen with with a very thin layer of carbon on it to prevent
charging would do the trick. Can anybody out there supply one? Thanks.

Alan Fox


Professor Alan G. Fox BSc PhD CEng FIM
Director, Center for Materials Science and Engineering
Naval Postgraduate School
Monterey
California 93943
USA

Tel (831) 656 2142 (work)
(831) 657 9239 (home)
Fax (831) 656 2238




From daemon Mon Apr 16 11:55:26 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Mon, 16 Apr 2001 12:19:46 -0400 (EDT)
Subject: EDX systems

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Dear listers,

This is an apology written in embarassment for some of my private comments
about EDX systems that recently got broadcast to the list. I had
too-quickly responded offline to a posting and was chagrined to find my
comments passed along without the balanced context I would have put them in
if I'd been more sensitive to the probability of dissemination. It's too
late to rephrase them but I want to emphasize that my recent demos with
Oxford, Noran, PGT and EDAX were all highly positive experiences and I came
away believing that all are solid and supportive companies offering world
class instrumentation. Any system will have many pros and a few cons; our
final choice will rest simply on which has a bigger cluster of the former
than the latter for our particular set of applications. The cons I had
mentioned were subjectively-perceived blips within the significent
strengths that each system offers. While cons can serve to pare down the
choice, once it's made, as I've discovered through discussion with
reference users representing all four companies, users across the board (at
least all those I've spoken to) are happy with whichever system they
purchased.

Dee






***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Mon Apr 16 12:29:25 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 16 Apr 2001 13:24:43 -0400
Subject: RE: Standard sample for EDS quant

Contents Retrieved from Microscopy Listserver Archives
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There is a decent test sample out there for checking and monitoring your EDS system called the NiOx sample. One place that you can find it is at Ted Pella. Here is the web site for that product. It has further information on it.
http://www.tedpella.com/calibrat_html/TEM7.htm

I am not sure what you mean by the accuracy of your EDS system. You will need to calibrate it and determine whether you are having problems with icing. You can do that with the NiOx sample. The literature that comes with the sample or that you can get from Ted Pella will tell you how to do this. It is also tells you how to monitor your system's performance over time.

If you want to check for N2, just get some Hexagonal BN, crush it between two glass slides, and collect it on a carbon coated grid. You will easily find thin particles.

You hit on a couple of problems with your request. You will have a difficult time ascertaining the thickness of any sample accurately, but with glass, you will only be limited to doing it with EELS or contamination spots. With glass, the composition can change under the beam as elements particularly alkali elements diffuse out of the irradiated area. The composition of glasses can change depending on the depth from the surface and so where you are can make a big difference. I do not know where you would get a glass with a N2 concentration. You can also cause the glass to soften in the beam in very thin areas if care is not taken. Higher accelerating voltages help here tremendously.

I have found that frequently I do not need to coat my glass samples in a 200 keV TEM. I did have to do it when I used a 100 keV machine. For cross section samples, I started using Si blanks as half of the sample and it seems to have eliminated heating and charging problems.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Alan Fox [mailto:fox-at-nps.navy.mil]
} Sent: Monday, April 16, 2001 12:50 PM
} To: MS listserver
} Subject: Standard sample for EDS quant
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
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} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} To all colleagues who supply standards for EDS work
} I need a standard sample of known thickness to check the
} accuracy of my TEM/EDS system during standardless quant. I need to
} include light elements (Z {11) as I have a Moxtek SUTW window on my
} detector. I guess an amorphous glass sample that contains
} both nitrogen
} and oxygen with with a very thin layer of carbon on it to prevent
} charging would do the trick. Can anybody out there supply one? Thanks.
}
} Alan Fox
}
}
} Professor Alan G. Fox BSc PhD CEng FIM
} Director, Center for Materials Science and Engineering
} Naval Postgraduate School
} Monterey
} California 93943
} USA
}
} Tel (831) 656 2142 (work)
} (831) 657 9239 (home)
} Fax (831) 656 2238
}
}
}


From daemon Mon Apr 16 12:57:53 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 16 Apr 2001 12:53:13 -0500
Subject: Posting summaries

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May I suggest that we consider some ground rules regarding postings of
summaries of replies to questions? It happens on occasion that replies
which are assumed to be made in private get posted publicly, sometimes to
the embarrassment of those doing the replying. This is, I'm sure, never
done with any bad intent and is usually harmless , but can nonetheless be a
little disconcerting.

I have posted summaries myself without thinking of the possible
consequences, so I'm not pointing any fingers. It's something that's easy
to forget about until you get caught yourself.

I would suggest as starting points that: 1) questioners always make clear
their intent to post a summary, 2) that the identities of the repliers be
removed from summaries (this is often done anyway), and 3) that all who
reply to a question indicate if they want their replies to be kept private.

Anyone else have any thoughts on this matter?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Mon Apr 16 13:23:53 2001



From: aram7486-at-qudsmail.com
Date: Sun, 15 Apr 2001 22:33:22 -0600
Subject: Are you looking for the perfect tax write off? 2858

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From daemon Mon Apr 16 15:40:22 2001



From: Michael Jarnik :      M_Jarnik-at-fccc.edu
Date: Mon, 16 Apr 2001 16:28:16 -0400
Subject: DNA in Cytochrome C films

Contents Retrieved from Microscopy Listserver Archives
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I would need to prepare DNA for TEM using spreading/shadowing in
Cytochrome C films. My pilot experiments using just plasmid DNA and the
Lang & Mitani method (Biopolymers, 9, p.373, 1970) worked rather poorly
and I would like to hear from people with some experience in this
method. What are the critical points here? Purity of water/chemicals,
Cyt C concentration, time? Any hints would be appreciated.

Thanks for help,

--
Michael Jarnik




From daemon Mon Apr 16 16:05:01 2001



From: PMarcum :      pmarcum-at-p3.net
Date: Mon, 16 Apr 2001 16:57:21 -0400
Subject: Fwd: TEM

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Do I understand you believe this is CJD or prion infected tissue? If so
several methods of disinfection have been suggested (using the word
disinfect loosely). No one is sure exactly what will inactivate this prion
and the procedure has been disgusted on Histonet for routine pathology
giving the latest suggested methods. I can attempt to forward some of that
information to you along with the website address. Let me know if you would
like the information. Most of us are alittle frightened of this one as it
can take years to manifest.
Pamela A. Marcum
Histology/Microscopy
Product Development Manager
400 Valley Road
Warrington, PA 18976
Phone: 800-523-2575 Ext 167
215-343-6484 Ext 167
Fax: 215-343-0214
E-mail: pmarcum-at-polysciences.com

-----Original Message-----
} From: Greg Erdos [mailto:gwe-at-biotech.ufl.edu]
Sent: Monday, April 16, 2001 9:17 AM
To: Microscopy-at-sparc5.microscopy.com


Please reply directly to:

} From: {hmskaug-at-tartarus.uwa.edu.au}
}
} Subject: TEM
}
}
} Hi,
}
} I am doing some preperation work for an assignment, and have a question.
}
} If a fresh unfixed piece of brain tissue (weighing approx. 1 gram) is
} received in a diagnostic electron microscopy laboratory, how would I
} proceed with the specimen? The brain is taken in surgery within the last 5
} min.
}
} If I was asked to carry out a rapid viral diagnosis on the tissue, what
} precautions should I take, and how would I proceed?
} I read that prions are able to survive fixation. What then?
}
} A respond is very much appreciated.
}
} Thank You !
}
} Sincerely,
}
} Hege Skaug

Greg Erdos
Assistant Director
Biotechnology Program Ph. 352-392-1295
University of Florida Fax 352-846-0251
PO Box 118525
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl



From daemon Mon Apr 16 16:41:05 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 16 Apr 2001 17:36:45 -0400
Subject: Re: Standard sample for EDS quant

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To all colleagues who supply standards for EDS work
I need a standard sample of known thickness to check the
accuracy of my TEM/EDS system during standardless quant. I need to
include light elements (Z {11) as I have a Moxtek SUTW window on my
detector. I guess an amorphous glass sample that contains both nitrogen
and oxygen with with a very thin layer of carbon on it to prevent
charging would do the trick. Can anybody out there supply one? Thanks.

Alan Fox

Dear Alan,
I can't get you a standard with all the qualifications you want, but I do
have some standards prepared by Chuck Fiori. The matrix is a lithium borate
glass, and there are several blocks with various elements evenly dispersed in
the matrix. The down side is that you will have to melt the glass, prepare thin
specimens--by blowing with a platinum straw--break off pieces from the bubble,
and put them on (or in) a suitable grid. I have found that folding grids work
well. Of course, you will have to measure the thickness after you prepare the
specimen. Anyway, it is amorphous glass with light elements, including oxygen.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Mon Apr 16 16:41:07 2001



From: wonger-at-allover.com
Date: Mon, 16 Apr 2001 16:38:52 -0500
Subject: Ask-A-Microscopist:Agar

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Email: wonger-at-allover.com
Name: Billone

Organization: Murphy

Education: 6-8th Grade Middle School

Location: San Jose, California

Question: How long should it take for agar to conjeal after I pour it
into a petri dish?

---------------------------------------------------------------------------


From daemon Mon Apr 16 18:23:56 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Tue, 17 Apr 2001 11:07:13 +1300
Subject: Visualising mineral oil - Thanks

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Thanks to all who replied.

We are trying out the suggestion of using Nile Red to increase
fluorescence. This seems very promising at this stage.

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


_________________________________________________________________
The contents of this e-mail are privileged and/or confidential to the named
recipient and are not to be used by any other person and/or organisation.
If you have received this e-mail in error, please notify the sender and delete
all material pertaining to this e-mail.
_________________________________________________________________


From daemon Mon Apr 16 18:23:57 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Tue, 17 Apr 2001 11:05:02 +1300
Subject: Fluorescence Stereomicroscopes

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We are looking at purchasing a stereo microscope with
fluorescence capabilities primarily to look at GFP fluorescence in
plant specimens. Does anyone have any particular comments on
the relative merits of the systems produced by the major
microscope manufactureres (Leica, Nikon, Olympus and Zeiss).
We also have a dichotomy amongst users on whether to provide
film or digital cameras for recording - my own preference is more on
the digital side but again has anyone any comments or
suggestions.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


_________________________________________________________________
The contents of this e-mail are privileged and/or confidential to the named
recipient and are not to be used by any other person and/or organisation.
If you have received this e-mail in error, please notify the sender and delete
all material pertaining to this e-mail.
_________________________________________________________________


From daemon Mon Apr 16 22:02:15 2001



From: Arthur Day :      ard-at-ansto.gov.au
Date: Tue, 17 Apr 2001 13:56:14 +1100
Subject: EDS: Ephemeral low energy tails

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Dear List,

This is a cry for help on behalf of an unwell EDS detector that has
been having some problems. It has been behaving very strangely lately
but does not know who to turn to....

For the past six months it has been suffering short-lived episodes of
low energy tails accompanied by some visible peak broadening most
apparent at the low energy end of the spectrum. I am aware of
previous discussions about possible causes of tails and peak
broadening but I think the unusual thing in this case is that the
problem only lasts for the first few minutes after the SEM beam is
turned on and the detector is first exposed to X-rays for the day. It
then gradually goes away and all aspects of the detector's behavior
return to normal again. The tails won't return again that day once
the SEM has been used.

This is a 10mm2 127eV Noran "Pioneer" detector with an atmospheric
thin window hooked up to a Voyager IV with digital pulse processing.
We leave the whole system running all of the time. It is attached to
a Jeol SEM and we normally use its airlock to change samples which
means that the crystal/window do not see daylight/atmospheric
pressure very often. We routinely use the "slowest" highest
resolution pulse processor settings. Except for this short-lived
problem everything else about the system is as good as it ever was
when we first installed it and ran the pulse processor setup routines
a few years ago. As far as we can tell there is no *detectable*
buildup of ice inside the detector (no phantom oxygen peaks etc) and
there is only a small amount of visible oil contamination on the
window.

It takes 12 hours or more of idleness before the tails re-appear,
where then it can take 10 to 20 minutes exposure to X-rays for them
to go away again. It takes less time for the tails to go away if the
beam current is temporarily ramped up high enough to "saturate" the
system with enough counts to approach 100% dead time. The only other
unusual features that we have observed after the detector has been
idle for a while are that the idle dead time indication fluctuates
wildly from zero to ~50% or more from reading to reading (two second
sampling periods) instead of the usual 10-20 percent, and the idle
Detects and Converts are only a few counts/sec instead of the normal
several tens of counts/sec. After a good dose of X-rays these
parameters return to their normal idle values again also.

There is another piece to this puzzle. Last week we gave the detector
a "photon enema" with a flashlight while examining the window and
surrounds for anything unusual and that had the same effect in curing
the problem as using a high beam current to generate X-rays.

So does anybody out there have some clues about this -- a cause, a
cure, or even the physics of it? I've got the impression that it must
be something electronically weird in relation to the crystal, such as
some sort of an excess charge accumulation when it is idle for a long
time which then gradually dissipates when the crystal starts
responding to X-ray photons again? Could this effect occur if the
bias was just slightly wrong by a few volts? How about "leakage
current" or FET problems? I haven't got the foggiest idea really
because the zero position and peak energies are always ok.

Very strange?

Concerned,
Australia.






Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Tue Apr 17 08:39:32 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 17 Apr 2001 08:31:58 -0500
Subject: Re: Fluorescence Stereomicroscopes

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Ian,
I have no specific comments with the new breed of fluorescent
stereo's. However, in going through this a decade ago with
"conventional" stereos, I found that different scopes though all well
designed ranked differently with different specimens. The objects
that we biologists stare at are odd optically. I suggest that you get
demos and inspect the favorite objects in your departement that will
be viewed under the scope. I found one stereo whose darkfield far
outshone the others for arabidopsis roots, though there is no
"rational" reason for this, and nothing to say that this scope is
"better" in general.

A key feature to insist on is a shunt with 100% of the light
to the phototube. In the old days most stereos did not do this,
perhaps with fluoresecence things have changed, but you want all the
light going for the captured image, whether it is film or pixels.

For image capture, going directly to a color slide can be
handy. On my stereo, we trade off between video and film quite a lot.
Our 35 mm camera back is no big deal (that is, just a cameraback on a
tube) and adding that capability did not add much to the cost. You
can of course buy much fancier and expensive 35 mm controllers but my
point is you don't have to.

Hope this helps,
Tobias


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Tue Apr 17 09:00:36 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 17 Apr 2001 08:48:36 -0400
Subject: Re: Ask-A-Microscopist:Agar

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Email: wonger-at-allover.com
Name: Billone

Organization: Murphy

Education: 6-8th Grade Middle School

Location: San Jose, California

Question: How long should it take for agar to conjeal after I pour it
into a petri dish?

Dear Billone,
Way back when I was pouring agar for immunodiffusion plates, I found that
it congealed almost immediately. I had some trouble keeping it liquid to get a
good smooth surface. Since that applies to the specific concentration of agar I
used (I forget, but I think it was ~1%) and the temperature it was heated to
(~40 C, I think), and since that was before global warming, YMMV.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Tue Apr 17 10:15:45 2001



From: Glen :      glenmac-at-u.washington.edu
Date: Tue, 17 Apr 2001 08:10:51 -0700
Subject: Re: Fluorescence Stereomicroscopes

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Dear Ian,
The recommendation from Tobias to compare before buying is always sound. We've
been using a Leica MZ-12 epi-fluorescent stereomicroscope for about 2 years. GFP
is only part of its use, we do a lot of microinjection and of late have been using
it with vitally stained zebrafish larvae. There are a number of practical
considerations. All of the mfrs. originally had limited ability to change
wavelengths, the original Leica required removing the eyepieces and camera adapter
as a unit, then removing screws to withdraw the filterset. Their current FL-III
system has a rotating ring holding several filtersets. Filter combinations may be
removed and inserted easily with it. We shuffle about 7 different sets between
the filter ring, which holds about 6 (and we keep one open for brightfield).
Don't ever think you will never need more than just GFP.

Adding a camera tube and fluorescent lamp housing makes the scope head very
heavy. We have ours on a double arm boom (Diagnostics Inst.) to maneuver
micromanipulators beneath it, and it still vibrates when you touch it. The focus
must be kept tight. I just bought a lab jack stand to support our samples and to
provide fine focus. Pay attention to flex in the manner in which the scope head
is attached to the focus mechanism, and to the boom.

With fluorescence, consider the NA of the lenses, as that will determine whether
the fluorescence is visible. We use a .8X/.05 NA for general use and long working
distance with microinjection equipment. But the 1X/.125 allows us to see GFP
transfected neuronal processes. A new 1.6X/.2 lens provides several times greater
intensity allowing some projects to be done quickly with the stereoscope instead
of having to be transferred to a compound scope.

I'd be interested in your digital camera decision. The weight issue limits what
we can install. Weve been sticking with a no-name single chip color camera, which
is very lightweight. The resolution is fair, and it is surprising light
sensitive. The users who prefer it are viewing the color output on a passthrough
monitor, then capture monochrome. The color is essential when doing multi-label
injections. Color capture resolution from it is dreadful, but the monochrome is
decent resolution. Other color cameras have been too heavy, inducing big
vibrations when you touch the focus, or have not been sensitive enough. A Nikon
Coolpix 990 is terrific for brightfield, but lacks sensitivity for fluorescence.
You can get pretty good at guesstimating exposure times for 1-8 second exposures
of dim fluorescence. But, the chip noise isn't worth it. And, if you need
real-time imaging of low-light images for any reason (there are several reasons
you might) then the Coolpix won't work. The final straw with the Coolpix, and
some other cameras, is that we use some far-red dyes, like DiD, to which the Nikon
is blind at working concentrations.

Regards,
Glen

}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } -----------------------------------------------------------------------.
} }
} }
} } We are looking at purchasing a stereo microscope with
} } fluorescence capabilities primarily to look at GFP fluorescence in
} } plant specimens. Does anyone have any particular comments on
} } the relative merits of the systems produced by the major
} } microscope manufactureres (Leica, Nikon, Olympus and Zeiss).
} } We also have a dichotomy amongst users on whether to provide
} } film or digital cameras for recording - my own preference is more on
} } the digital side but again has anyone any comments or
} } suggestions.
} }
} } Thanks
} }
} } Ian
} }
} }
} } Ian Hallett
} } HortResearch
} } Mt Albert Research Centre
} } Private Bag 92 169
} } Auckland, New Zealand
} } Fax 64-9-815 4201
} } Telephone 64-9-815 4200
} } EMail ihallett-at-hortresearch.co.nz
} }
}

-- Glen MacDonald
UW Core for Communications Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156



From daemon Tue Apr 17 11:28:57 2001



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Tue, 17 Apr 2001 09:24:11 -0700 (PDT)
Subject: Re: Standard sample for EDS quant

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Alan - The National Institute of Standands and Technology (NIST) sells a
well-characterized Mg-Si-Ca-Fe-O glass thin film which is intended as a
standard for EDS calibration in the TEM. The glass is supported by a 20
nm carbon thin film and its thickness has been measured by profilometry.
I routinely use this standard along with other mineral standards for EDS
calibration in the TEM and over the years have found it to be a very good
standard indeed.

NIST can be contacted at (301)975-6776.

Dave

Dave Joswiak
Dept. of Astronomy, 351580
University of Washington
Seattle, WA 98195
(206)543-7702



On Mon, 16 Apr 2001, Alan Fox wrote:

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}
}
} To all colleagues who supply standards for EDS work
} I need a standard sample of known thickness to check the
} accuracy of my TEM/EDS system during standardless quant. I need to
} include light elements (Z {11) as I have a Moxtek SUTW window on my
} detector. I guess an amorphous glass sample that contains both nitrogen
} and oxygen with with a very thin layer of carbon on it to prevent
} charging would do the trick. Can anybody out there supply one? Thanks.
}
} Alan Fox
}
}
} Professor Alan G. Fox BSc PhD CEng FIM
} Director, Center for Materials Science and Engineering
} Naval Postgraduate School
} Monterey
} California 93943
} USA
}
} Tel (831) 656 2142 (work)
} (831) 657 9239 (home)
} Fax (831) 656 2238
}
}
}
}




From daemon Tue Apr 17 11:52:40 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Tue, 17 Apr 2001 11:48:59 -0500
Subject: Re: Ask-A-Microscopist:Agar

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Hi,
Your agar should be setting up almost immediately. I usually use a
concentration of 1-2% for my purposes. One imperative point is that the
agar needs to be completely dissolved. This may sound like a trivial
comment, but I know that when I first started working with agar I made the
mistake of not heating the solution hot enough for a long enough time and
wondered what was wrong. If you have access to an autoclave, autoclave it
for about 15 minutes. If not, heat it on a hot plate (preferably with a
stir bar, but you can swirl it periodically if you don't have a stir bar)
until the agar is completely dissolved and you have a nice, clear solution.
Be careful, because agar can suddenly start to boil and be up and over the
top of your flask before you realize it.
Let me know if you need any more help.
Good luck,
Kristen

At 04:38 PM 4/16/01 -0500, you wrote:
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Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu



From daemon Tue Apr 17 13:06:45 2001



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Tue, 17 Apr 2001 13:59:34 -0400
Subject: Unsubscribe

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Please unsubscribe me. Thank you.



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Tue Apr 17 13:24:04 2001



From: jfb :      jfb-at-uidaho.edu
Date: Tue, 17 Apr 2001 11:20:19 -0700
Subject: West Coast Amray Service

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Does anyone know of service labs on the West coast for Amray
instruments? I would appreciate knowing if any exists.
Thank you.

Franklin Bailey
University of Idaho
Moscow, ID 83844-2204
jfb-at-uidaho.edu



From daemon Tue Apr 17 14:03:18 2001



From: RCHIOVETTI-at-aol.com
Date: Tue, 17 Apr 2001 14:58:51 EDT
Subject: Re: Fluorescence Stereomicroscopes

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 04/17/2001 8:23:01 AM US Mountain Standard Time,
glenmac-at-u.washington.edu writes:

{ { Adding a camera tube and fluorescent lamp housing makes the scope head very
heavy. We have ours on a double arm boom (Diagnostics Inst.) to maneuver
micromanipulators beneath it, and it still vibrates when you touch it. The
focus
must be kept tight. I just bought a lab jack stand to support our samples
and to
provide fine focus. Pay attention to flex in the manner in which the scope
head
is attached to the focus mechanism, and to the boom.
} }

Glen's message is full of great advice. Regarding the point mentioned above,
this is indeed crucial as you start stacking photo tubes, ergo heads,
cameras, etc. onto the stereomicroscope. You can easily overwhelm the load
limits of the focus drive and the gearing, and the scope will start to
"droop" so that you have to continually bring the scope back into focus.

I don't know about other scopes, but Leica has a tension upgrade kit that can
be installed in the focus drive unit for these situations. It makes the
focus much tighter. This upgrade is for the MZ FL III which is configured
(usually) on a transmitted light base with a focus post and focus drive
attached to the base. As far as I know, there is no such kit if you decide
to put the scope on a boom stand or a swinging arm stand. In that case, you
would have to deal with the tightness of the articulated joints on the stand.

Anyway, if you order the MZ FL III stereofluorescence scope in the usual
configuration, Leica has a cure for the "droopy scope" syndrome if it gets
too heavy. For details on the upgrade you can contact Leica Customer Service
at 1-800-248-0123. Or contact me and I will put you in touch with our
Service Engineers who have installed several of the tension upgrade kits.
One qualifier: installing the kit and the springs requires disassembling the
focus drive unit. A factory trained service guy should definitely do this.

Good luck!

Best regards,

Bob Chiovetti
GTI Microsystems, Inc.
Leica Exclusive Regional Dealer
Southwestern United States


From daemon Tue Apr 17 15:27:08 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 17 Apr 2001 16:21:06 -0400
Subject: Film Processing and dynamic range

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Colleagues-

I'm going out on a limb here, because it is not a particular area of
expertise of mine, but I've not seen it in the discussion. Experts in
microscopy of radiation sensitive materials are much more familiar with
these issues (Linn Hobbs - can you help us here?)

I'm surprised that no-one has pointed out a very important difference
between exposure of films to photons and electrons. Film exposed to
photons exhibits a logarithmic response - that is, D varies as
log(exposure). For electrons, this is not the case, but instead, the
density is linear with exposure. This arises because individual grains
require exposure to many photons to make them "developable", whereas a
single electron will *COMPLETELY* expose many grains. For brevity, I will
not explain how this changes the exposure characteristics, but it does.

A second consequence of this difference is that while change of development
can change the threshold exposure for development of grains sensitised by
light, grains exposed to electrons are either fully sensitized or are
completely virgin, and there is *FAR* less scope to change the image
characteristics by changing development of electron-exposed emulsions
(though there is some, for Kodak SO163, for example).

This makes correct exposure much more critical for electron images, and
makes them much more prone to overexposure. With light, and area with D of
4.0 has received 10,000 times more light than an area with D of 1.0. In an
electron image the area with D of 4.0 has received 4 times more electrons
than the area with D of 1.0. Do negatives really have D's above 4?
Certainly they can when exposed to electrons.

There are other consequences. For example, the contrast (as we usually
define it as the difference in density between different areas), which is
independant of exposure on the linear portion of a film's response for
light (as explained by Gary Gaugler), is, in the case of electrons, a
linear function of the exposure. Underexposure leads to loss of contrast.
This is why images of radiation-sensitive materials are taken at low
magnification - it is the only way to maintain enough exposure of the
emulsion to give acceptable contrast, without increasing the electron dose
on the sample. Incidentally, the limit on information in such images is
probably not the "grain size" of the emulsion, but the shot noise due to
the finite number of electrons used to generate the image.

There is much more to this topic - and I have simplified what I have said.

Tony



* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Tue Apr 17 15:39:49 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 17 Apr 2001 16:31:50 -0400 (EDT)
Subject: Re: Ask-A-Microscopist:Agar

Contents Retrieved from Microscopy Listserver Archives
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Kristen is right on, especially with the safety issues. The completely
dissolved agar should not look like it has sand in it when you swirl the
flask. Be careful when swirling; if it is near the poiling point, it
will foam up over the top before you can set it down. It can cause nasty
burns like hot oil because it is thick.

Agar is a complex polysaccharide extracted from algae (several
genera in the Rhodoophyceae). It melts somewhere around 96 degrees C and
solidifies around 36 degrees C. I can't quite remember exact
temperatures. Thus, to answer your solidification question, it will
solidify when it gets to about body temperature. The length of time
that takes after pouring will depend on how hot it was when it was
poured, how thick your pour it, and how cool the Petri plate and room are.

Address at bottom if you have further questions.


On Tue, 17 Apr 2001, Kristen Lennon wrote:

} Date: Tue, 17 Apr 2001 11:48:59 -0500
} From: Kristen Lennon {kalen-at-iastate.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Ask-A-Microscopist:Agar
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} Your agar should be setting up almost immediately. I usually use a
} concentration of 1-2% for my purposes. One imperative point is that the
} agar needs to be completely dissolved. This may sound like a trivial
} comment, but I know that when I first started working with agar I made the
} mistake of not heating the solution hot enough for a long enough time and
} wondered what was wrong. If you have access to an autoclave, autoclave it
} for about 15 minutes. If not, heat it on a hot plate (preferably with a
} stir bar, but you can swirl it periodically if you don't have a stir bar)
} until the agar is completely dissolved and you have a nice, clear solution.
} Be careful, because agar can suddenly start to boil and be up and over the
} top of your flask before you realize it.
} Let me know if you need any more help.
} Good luck,
} Kristen
}
} At 04:38 PM 4/16/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } -----------------------------------------------------------------------.
} }
} }
} }
} } Email: wonger-at-allover.com
} } Name: Billone
} }
} } Organization: Murphy
} }
} } Education: 6-8th Grade Middle School
} }
} } Location: San Jose, California
} }
} } Question: How long should it take for agar to conjeal after I pour it into
} } a petri dish?
} }
} } ---------------------------------------------------------------------------
}
} Kristen A. Lennon, Ph.D.
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
} 515-294-8854
} kalen-at-iastate.edu
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Apr 17 15:41:08 2001



From: Michael Pidgeon :      pidgeon-at-hsc.usc.edu
Date: Tue, 17 Apr 2001 13:38:23 -0700
Subject: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All.

I have been trying to locate a point source bulb for my Durst enlarger,
It is GE bulb BHD it is a single contact bayonet type that is a 100 W ,
20V bulb , which I have been told is an old microscope bulb that has
been discontinued with no replacement noted. If anyone knows of or has
a source where I can get a suitable replacement or have that bulb,
please let me know.

Thanks,

Michael Pidgeon
Keck School of Medicine at USC
Dept. of Cell & Neurobiology

323-442-1862



From daemon Tue Apr 17 15:41:08 2001



From: Michael Pidgeon :      pidgeon-at-hsc.usc.edu
Date: Tue, 17 Apr 2001 13:40:09 -0700
Subject: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All.

I have been trying to locate a point source bulb for my Durst enlarger,
It is GE bulb BHD it is a single contact bayonet type that is a 100 W ,
20V bulb , which I have been told is an old microscope bulb that has
been discontinued with no replacement noted. If anyone knows of or has
a source where I can get a suitable replacement or have that bulb,
please let me know.

Thanks,

Michael Pidgeon
Keck School of Medicine at USC
Dept. of Cell & Neurobiology

323-442-1862



From daemon Tue Apr 17 15:51:57 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Tue, 17 Apr 2001 13:47:48 -0700 (PDT)
Subject: scheduling software

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
I've written some software in Perl that may be of use in laboratory
facilities. We use it to schedule and monitor usage of our TEM. It
simulates the old sign up sheet on the wall idea, but on the web. It has
a modest amount of security to control which users can sign up onto the
schedule. It also keeps a searchable archive of your past schedules for
history and billing information.

I've tried to make the installation as automated as possible. You'll need
to have an account on a server where you can run cgi scripts, or your own
linux/sun/irix/windows nt server. You'll also need to have Perl installed
where you want the software to run. Please send me comments if you have
any difficulties with setting up or running the software. I'm at version
1.2 which hopefully shouldn't have too many bugs.

The link for the software is at:
http://wilfred.berkeley.edu/~gordon/www-sched
I ask for a small fee for our lab for the software which is explained on
the web page.
Gordon Vrdoljak.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Tue Apr 17 16:15:17 2001



From: Alan Fox :      fox-at-nps.navy.mil
Date: Tue, 17 Apr 2001 14:12:05 -0700
Subject: Calibration standard for EDS in the TEM

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everyone who replied concerning my request for an EDS
standardless quant. calibration standard for the TEM. NIST indeed do
sell a mineral glass standard for just this purpose and I am looking
into purchasing one of these. Thanks again for your help.

Alan Fox



From daemon Tue Apr 17 19:25:59 2001



From: Maria Fazio-Zanakis :      Maria.Fazio-Zanakis-at-unilever.com
Date: Tue, 17 Apr 2001 19:23:11 -0500
Subject: Re: DNA in Cytochrome C films

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Dear Michael,

I have done some very nice preps using the Kleinschmit method. The procedure
is a bit lengthy so please contact me off line and I would be more than happy
to give it to you...It has some modifications but it works very nicely.

Sincerely,
Maria

Maria Fazio-Zanakis
AIM - TEM Laboratory
Unilever Research, U.S.
45 River Road
Edgewater, NJ 07020
1-201-840-2287
Maria.Fazio-Zanakis-at-unilever.com


From daemon Tue Apr 17 19:39:29 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 17 Apr 2001 20:33:36 -0400 (EDT)
Subject: Re: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
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Try Bulbtronics. Sorry, I don't know address, but I think they have a
web site. They have all sorts of odd bulbs. Good luck.

On Tue, 17 Apr 2001, Michael Pidgeon wrote:

} Date: Tue, 17 Apr 2001 13:40:09 -0700
} From: Michael Pidgeon {pidgeon-at-hsc.usc.edu}
} To: MS listserver {Microscopy-at-sparc5.microscopy.com}
} Subject: Help locating replacment bulb
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello All.
}
} I have been trying to locate a point source bulb for my Durst enlarger,
} It is GE bulb BHD it is a single contact bayonet type that is a 100 W ,
} 20V bulb , which I have been told is an old microscope bulb that has
} been discontinued with no replacement noted. If anyone knows of or has
} a source where I can get a suitable replacement or have that bulb,
} please let me know.
}
} Thanks,
}
} Michael Pidgeon
} Keck School of Medicine at USC
} Dept. of Cell & Neurobiology
}
} 323-442-1862
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Apr 18 06:31:48 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 18 Apr 2001 12:21:58 +0100
Subject: Re: DNA in Cytochrome C films

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Michael

I will send you separately details of the method that I used on
bacterial plasmids about 10-11 years ago (the method includes the
preliminary stages as well as the technique). I can't send this to the
list because the printed schedules need to be sent as attachments.

What I discovered was that it took several goes to get it right but the
most important thing to avoid was 'surface active agents' which prevent
a good film from spreading. Real 'double distilled water' is essential
(not de-ionised or single distilled) and it should be stored in glass
containers with no lubricants or plastic components (ground glass
bottles or aluminium foil to prevent contact with plastic caps). The
next was cleanliness - all glassware needs to be thoroughly soaked in a
cleaning agent and then carefully rinsed with no use of detergents (I
think some methods recommend chromic acid, but I found that a mixture of
2N (2M) nitric and 2N (1M) sulphuric acids were sufficient and a bit
safer - NB obviously take care adding acids to water and do in a fume
hood). Finally the 'hyperphase' containing the DNA should be mixed only
a minute or 2 before use. Obviously to avoid contaminants it is based to
keep a stock of chemicals just for this technique - but there aren't
that many and they aren't particularly expensive.

Useful equipment would include a teflon 'Langmuir trough' to perform the
spread (although I found that a square plastic dish worked well if
thoroughly washed) and a rotary shadowing stage for the vacuum coater
(although I just shadowed from two different angles at 90 deg of
rotation - it takes longer because you have to return to air twice).

Once the technique was perfected I managed to get several dozen
undergraduates to prepare DNA spreads of their plasmids, so it seemed
like a fairly robust method where the important bit was the preparation
before the DNA spread technique and a bit of practice. I found that in
order for the students to get a representative number of plasmids it was
best to photograph at about 10k and enlarge by 6x to 10x. I also found
that the DNA was easiest to spot by increasing on-screen contrast in the
microscope (smallest objective aperture and as low as 40kv electrons).

Malcolm

Malcolm Haswell
e.m. unit
University of Sunderland
UK

Michael Jarnik wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would need to prepare DNA for TEM using spreading/shadowing in
} Cytochrome C films. My pilot experiments using just plasmid DNA and the
} Lang & Mitani method (Biopolymers, 9, p.373, 1970) worked rather poorly
} and I would like to hear from people with some experience in this
} method. What are the critical points here? Purity of water/chemicals,
} Cyt C concentration, time? Any hints would be appreciated.
}
} Thanks for help,
}
} --
} Michael Jarnik


From daemon Wed Apr 18 07:12:22 2001



From: donald j marshall :      dmrelion-at-world.std.com
Date: Wed, 18 Apr 2001 08:08:18 -0400 (EDT)
Subject: Re: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
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Michael, The contact for Bulbtronics is 1-800-654-8542 or
www.bulbtronics.com. They have a wide range of bulbs, including a 12 page
catalog on just bulbs for microscopes.

I also have a file on a company called Bulb Direct. 1-800-772-5267
www.bulbdirect.com. They also have an extensive catalog.

I have no financial or other interest.............

Don Marshall


} From Microscopy-request-at-sparc5.microscopy.com Tue Apr 17 16:42:12 2001


Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."


}
} Hello All.
}
} I have been trying to locate a point source bulb for my Durst enlarger,
} It is GE bulb BHD it is a single contact bayonet type that is a 100 W ,
} 20V bulb , which I have been told is an old microscope bulb that has
} been discontinued with no replacement noted. If anyone knows of or has
} a source where I can get a suitable replacement or have that bulb,
} please let me know.
}
} Thanks,
}
} Michael Pidgeon
} Keck School of Medicine at USC
} Dept. of Cell & Neurobiology
}
} 323-442-1862
}
}



From daemon Wed Apr 18 07:59:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 18 Apr 2001 05:58:01 -0700
Subject: Re: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try http://www.bulbman.com

They have an awesome selection of all sorts of bulbs at
very good prices.

gary g.


At 01:38 PM 4/17/2001, you wrote:

} Hello All.
}
} I have been trying to locate a point source bulb for my Durst enlarger,
} It is GE bulb BHD it is a single contact bayonet type that is a 100 W ,
} 20V bulb , which I have been told is an old microscope bulb that has
} been discontinued with no replacement noted. If anyone knows of or has
} a source where I can get a suitable replacement or have that bulb,
} please let me know.
}
} Thanks,
}
} Michael Pidgeon
} Keck School of Medicine at USC
} Dept. of Cell & Neurobiology
}
} 323-442-1862



From daemon Wed Apr 18 08:11:36 2001



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Wed, 18 Apr 2001 09:07:10 -0400
Subject: Re: Film Processing and dynamic range

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Dear Tony Garratt-Reed,

your argument about film density being linear with exposure for
electrons came somewhat as a surprise. As you said, a single
electron will *COMPLETELY* expose many grains. So, if the next
electrons hits close to that area, some of the grains that would
normally become developable are already completely exposed, thus the
density of the film can not double and thus the response of the film
is not linear.

There is a very simple test for the linearity of film if you have
access to a field emission TEM with a biprism, or if you have a clean
two beam case for high resolution. If you do a Fourier transform of
just a cos-pattern, you should see two peaks in the Fourier
transform. You can look this up in any handbook on Fourier
transforms. If you do the same with a cos-pattern recorded on film,
you will see plenty of higher order peaks in Fourier space. If you
do this with a CCD camera, you will almost have to saturate the
pixels to detect higher order terms. CCD cameras are, contrary to
film, extremely linear.

If you have access to a film with a linear response, I would be eager
to learn about it. Otherwise, for more details on my arguments, I
would point to "Density Correction of Photographic Material ....",
Ultramicroscopy, 55 (1994) 75-90. It gives examples of several films
tested, the equations describing the behavior and tips how to
linearize the data from film with software once the data are
digitized.

With best regards,

Edgar Voelkl




} ------------------------------------------------------------------------
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--


________________
Dr. Edgar Voelkl
Senior Development Staff Member
ORNL
Bldg 4515, MS 6064
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov


From daemon Wed Apr 18 09:25:21 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Wed, 18 Apr 2001 10:23:27 -0400
Subject: Re: Ask-A-Microscopist:Agar

Contents Retrieved from Microscopy Listserver Archives
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Agar melts at 95 C and sets at ~40 C. If one melts agar (1-2%) directly on a hot plate, it can boil over when the temperature gets too high. However, if it is melted in a hot water bath, it will not boil over because the temperature will never get higher than 100 C.

How long does it take to set? It depends on the temperature of liquid agar when you pour it. If you pour 50 C agar, it will set immediately.







Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "William F. Tivol" {wft03-at-health.state.ny.us} 04/17 8:48 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America







Email: wonger-at-allover.com
Name: Billone

Organization: Murphy

Education: 6-8th Grade Middle School

Location: San Jose, California

Question: How long should it take for agar to conjeal after I pour it
into a petri dish?

Dear Billone,
Way back when I was pouring agar for immunodiffusion plates, I found that
it congealed almost immediately. I had some trouble keeping it liquid to get a
good smooth surface. Since that applies to the specific concentration of agar I
used (I forget, but I think it was ~1%) and the temperature it was heated to
(~40 C, I think), and since that was before global warming, YMMV.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us






From daemon Wed Apr 18 09:35:29 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 18 Apr 2001 10:31:45 -0400
Subject: Re: Film Processing and dynamic range

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Dear Tony,

For electrons, this is not the case, but instead, the
density is linear with exposure.

The only thing I will add to your consise explanation is that the density
is linear with exposure only over a limited portion of the total dynamic range.
The best films are linear for ODs from 0 to ~2, whereas the dynamic range is ~4
for these films. If one wants to determine the electron dose for the darker
areas of the film, one must take a series of exposures to known doses of
electrons and produce a curve of OD vs dose.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Wed Apr 18 10:41:20 2001



From: Christine Fitzgerald :      Christine.Fitzgerald-at-emitech.co.uk
Date: Wed, 18 Apr 2001 16:36:11 +0100
Subject: Agfa Pan Film.

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We are trying to source a supplier (other than Kodak who require us to
purchase large quantities) for the film Agfa Pan APX 100 35mm Film.

Any information would be appreciated. Please contact us off-line.

Thanks for your assistance.



Christine Fitzgerald
Emitech Ltd.
E-mail: christine.fitzgerald-at-emitech.co.uk



From daemon Wed Apr 18 10:52:32 2001



From: NPGSlithography-at-aol.com
Date: Wed, 18 Apr 2001 11:48:31 EDT
Subject: Re: West Coast Amray Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Does anyone know of service labs on the West coast for Amray
} instruments? I would appreciate knowing if any exists.

A list of independent SEM service providers can be found at
"www.jcnabity.com\service.htm".

Note that in some cases, these companies will service microscopes that are a
significant distance from the company location. If a service area is not
listed, you should contact the company to find out their service area.

If anyone has any additions or modifications that can be made to this list,
please let me know.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Wed Apr 18 11:23:18 2001



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Wed, 18 Apr 2001 11:15:37 -0500
Subject: Re: Help locating replacment bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,
For replacement bulbs of all kinds try:

1) Interlight Specialty Bulbs 1-800-743-0005 or
2) Second Source 1-800-776-3924

I have no personal financial interest in these companies.

Dean Abel
Biological Sciences
University of Iowa
Iowa City IA 52242

:
:
:
:
:
:



From daemon Wed Apr 18 11:27:29 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 18 Apr 2001 11:25:09 -0500 (CDT)
Subject: Re: Film Processing and dynamic range

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry,
Tony is correct about the linearity of film, at least after
digitization. We have done this test many times, and provided the film
is not grossly overexposed, the average exposure time and digitized
counts are a straight line. (There is a small offset which you can
correct for and is probably associated with our microdensitometer
electronics rather than real.)
Of course, if you push the film developing, you probably lost
the linearity.


On Wed, 18 Apr 2001, Dr. Edgar Voelkl wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Tony Garratt-Reed,
}
} your argument about film density being linear with exposure for
} electrons came somewhat as a surprise. As you said, a single
} electron will *COMPLETELY* expose many grains. So, if the next
} electrons hits close to that area, some of the grains that would
} normally become developable are already completely exposed, thus the
} density of the film can not double and thus the response of the film
} is not linear.
}
} There is a very simple test for the linearity of film if you have
} access to a field emission TEM with a biprism, or if you have a clean
} two beam case for high resolution. If you do a Fourier transform of
} just a cos-pattern, you should see two peaks in the Fourier
} transform. You can look this up in any handbook on Fourier
} transforms. If you do the same with a cos-pattern recorded on film,
} you will see plenty of higher order peaks in Fourier space. If you
} do this with a CCD camera, you will almost have to saturate the
} pixels to detect higher order terms. CCD cameras are, contrary to
} film, extremely linear.
}
} If you have access to a film with a linear response, I would be eager
} to learn about it. Otherwise, for more details on my arguments, I
} would point to "Density Correction of Photographic Material ....",
} Ultramicroscopy, 55 (1994) 75-90. It gives examples of several films
} tested, the equations describing the behavior and tips how to
} linearize the data from film with software once the data are
} digitized.
}
} With best regards,
}
} Edgar Voelkl
}
}
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Colleagues-
} }
} } I'm going out on a limb here, because it is not a particular area of
} } expertise of mine, but I've not seen it in the discussion. Experts in
} } microscopy of radiation sensitive materials are much more familiar with
} } these issues (Linn Hobbs - can you help us here?)
} }
} } I'm surprised that no-one has pointed out a very important difference
} } between exposure of films to photons and electrons. Film exposed to
} } photons exhibits a logarithmic response - that is, D varies as
} } log(exposure). For electrons, this is not the case, but instead, the
} } density is linear with exposure. This arises because individual grains
} } require exposure to many photons to make them "developable", whereas a
} } single electron will *COMPLETELY* expose many grains. For brevity, I will
} } not explain how this changes the exposure characteristics, but it does.
} }
} } A second consequence of this difference is that while change of development
} } can change the threshold exposure for development of grains sensitised by
} } light, grains exposed to electrons are either fully sensitized or are
} } completely virgin, and there is *FAR* less scope to change the image
} } characteristics by changing development of electron-exposed emulsions
} } (though there is some, for Kodak SO163, for example).
} }
} } This makes correct exposure much more critical for electron images, and
} } makes them much more prone to overexposure. With light, and area with D of
} } 4.0 has received 10,000 times more light than an area with D of 1.0. In an
} } electron image the area with D of 4.0 has received 4 times more electrons
} } than the area with D of 1.0. Do negatives really have D's above 4?
} } Certainly they can when exposed to electrons.
} }
} } There are other consequences. For example, the contrast (as we usually
} } define it as the difference in density between different areas), which is
} } independant of exposure on the linear portion of a film's response for
} } light (as explained by Gary Gaugler), is, in the case of electrons, a
} } linear function of the exposure. Underexposure leads to loss of contrast.
} } This is why images of radiation-sensitive materials are taken at low
} } magnification - it is the only way to maintain enough exposure of the
} } emulsion to give acceptable contrast, without increasing the electron dose
} } on the sample. Incidentally, the limit on information in such images is
} } probably not the "grain size" of the emulsion, but the shot noise due to
} } the finite number of electrons used to generate the image.
} }
} } There is much more to this topic - and I have simplified what I have said.
} }
} } Tony
} }
} }
} }
} } * * * * * * * * * * * * * * * * * * * * * * * * * *
} } * Anthony J. Garratt-Reed M.A., D.Phil.
} } * MIT, Room 13-1027
} } * 77 Massachusetts Avenue
} } * Cambridge, MA 02139-4307
} } * USA
} } * Phone: (617) 253-4622
} } * Fax: (617) 258-6478
} } *
}
} --
}
}
} ________________
} Dr. Edgar Voelkl
} Senior Development Staff Member
} ORNL
} Bldg 4515, MS 6064
} 1 Bethel Valley Road
} P.O. Box 2008
} Oak Ridge, TN 37831-6064
}
} Tel.: (865) 574-8181
} Fax: (865) 574-4913
} email: vog-at-ornl.gov
}
}

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Apr 18 12:47:01 2001



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Wed, 18 Apr 2001 13:36:27 -0400
Subject: Re: Film Processing and dynamic range

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sorry too :)

however I feel quite confident that I can stand behind my
experimental results. And I can assure you that film developing was
not pushed. In addition, when digitizing film, you get a second
non-linear function involved. Right? You can check this by using a
Kodak photographic step tablet no.3. They are available off the
shelf (at least used to be) and are calibrated. Density range is 21
steps from 0.05 to 3.05. But, maybe your microdensitometer is
already corrected for the transmittivity curve (Pixel value P = a
10**S, where a corresponds to the illumination intensity and S is the
density of the film) ?

Maybe, we are just talking degrees of linearity here? So, whatever
you have may be sufficient in some cases, but no in others?

Edgar



}
} Sorry,
} Tony is correct about the linearity of film, at least after
} digitization. We have done this test many times, and provided the film
} is not grossly overexposed, the average exposure time and digitized
} counts are a straight line. (There is a small offset which you can
} correct for and is probably associated with our microdensitometer
} electronics rather than real.)
} Of course, if you push the film developing, you probably lost
} the linearity.
}
}
} On Wed, 18 Apr 2001, Dr. Edgar Voelkl wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Tony Garratt-Reed,
} }
} } your argument about film density being linear with exposure for
} } electrons came somewhat as a surprise. As you said, a single
} } electron will *COMPLETELY* expose many grains. So, if the next
} } electrons hits close to that area, some of the grains that would
} } normally become developable are already completely exposed, thus the
} } density of the film can not double and thus the response of the film
} } is not linear.
} }
} } There is a very simple test for the linearity of film if you have
} } access to a field emission TEM with a biprism, or if you have a clean
} } two beam case for high resolution. If you do a Fourier transform of
} } just a cos-pattern, you should see two peaks in the Fourier
} } transform. You can look this up in any handbook on Fourier
} } transforms. If you do the same with a cos-pattern recorded on film,
} } you will see plenty of higher order peaks in Fourier space. If you
} } do this with a CCD camera, you will almost have to saturate the
} } pixels to detect higher order terms. CCD cameras are, contrary to
} } film, extremely linear.
} }
} } If you have access to a film with a linear response, I would be eager
} } to learn about it. Otherwise, for more details on my arguments, I
} } would point to "Density Correction of Photographic Material ....",
} } Ultramicroscopy, 55 (1994) 75-90. It gives examples of several films
} } tested, the equations describing the behavior and tips how to
} } linearize the data from film with software once the data are
} } digitized.
} }
} } With best regards,
} }
} } Edgar Voelkl
} }
} }
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Colleagues-
} } }
} } } I'm going out on a limb here, because it is not a particular area of
} } } expertise of mine, but I've not seen it in the discussion. Experts in
} } } microscopy of radiation sensitive materials are much more familiar with
} } } these issues (Linn Hobbs - can you help us here?)
} } }
} } } I'm surprised that no-one has pointed out a very important difference
} } } between exposure of films to photons and electrons. Film exposed to
} } } photons exhibits a logarithmic response - that is, D varies as
} } } log(exposure). For electrons, this is not the case, but instead, the
} } } density is linear with exposure. This arises because individual grains
} } } require exposure to many photons to make them "developable", whereas a
} } } single electron will *COMPLETELY* expose many grains. For brevity, I will
} } } not explain how this changes the exposure characteristics, but it does.
} } }
} } } A second consequence of this difference is that while change of development
} } } can change the threshold exposure for development of grains sensitised by
} } } light, grains exposed to electrons are either fully sensitized or are
} } } completely virgin, and there is *FAR* less scope to change the image
} } } characteristics by changing development of electron-exposed emulsions
} } } (though there is some, for Kodak SO163, for example).
} } }
} } } This makes correct exposure much more critical for electron images, and
} } } makes them much more prone to overexposure. With light, and area with D of
} } } 4.0 has received 10,000 times more light than an area with D of 1.0. In an
} } } electron image the area with D of 4.0 has received 4 times more electrons
} } } than the area with D of 1.0. Do negatives really have D's above 4?
} } } Certainly they can when exposed to electrons.
} } }
} } } There are other consequences. For example, the contrast (as we usually
} } } define it as the difference in density between different areas), which is
} } } independant of exposure on the linear portion of a film's response for
} } } light (as explained by Gary Gaugler), is, in the case of electrons, a
} } } linear function of the exposure. Underexposure leads to loss of contrast.
} } } This is why images of radiation-sensitive materials are taken at low
} } } magnification - it is the only way to maintain enough exposure of the
} } } emulsion to give acceptable contrast, without increasing the electron dose
} } } on the sample. Incidentally, the limit on information in such images is
} } } probably not the "grain size" of the emulsion, but the shot noise due to
} } } the finite number of electrons used to generate the image.
} } }
} } } There is much more to this topic - and I have simplified what I have said.
} } }
} } } Tony
} } }
} } }
} } }
} } } * * * * * * * * * * * * * * * * * * * * * * * * * *
} } } * Anthony J. Garratt-Reed M.A., D.Phil.
} } } * MIT, Room 13-1027
} } } * 77 Massachusetts Avenue
} } } * Cambridge, MA 02139-4307
} } } * USA
} } } * Phone: (617) 253-4622
} } } * Fax: (617) 258-6478
} } } *
} }
} } --
} }
} }
} } ________________
} } Dr. Edgar Voelkl
} } Senior Development Staff Member
} } ORNL
} } Bldg 4515, MS 6064
} } 1 Bethel Valley Road
} } P.O. Box 2008
} } Oak Ridge, TN 37831-6064
} }
} } Tel.: (865) 574-8181
} } Fax: (865) 574-4913
} } email: vog-at-ornl.gov
} }
} }
}
} -------------------------------------------------------
} Laurence Marks
} Department of Materials Science and Engineering &
} Center for Transportation Nanotechnology
} Northwestern University
} Tel: (847) 491-3996 Fax: (847) 491-7820
} mailto:ldm-at-risc4.numis.nwu.edu
} http://www.numis.nwu.edu http://www.ctn.northwestern.edu
} -------------------------------------------------------
} The Other Nanotubes http://focus.aps.org/open/st12.html
} Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html
}
} Workshop May 17-19 2001 "New approaches to the Phase Problem"
} http://xraysweb.lbl.gov/esg/phasing/index.html

--


________________
Dr. Edgar Voelkl
Senior Development Staff Member
ORNL
Bldg 4515, MS 6064
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov


From daemon Wed Apr 18 13:43:40 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 18 Apr 2001 13:40:39 -0500 (CDT)
Subject: Re: Film Processing and dynamic range

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very often it seems that film is described as non-linear, direct CCD
collectors are linear, so bye-bye film. Since we did not then (and
still do not have) a good CCD detector on our UHV-HREM, we investigated
this in detail some years ago to get quantitative TED data from
surfaces. While there are of course logarithmic terms involved, with a
good drum scanner the electronics to handle this works well. If anyone
wants we have calibration curves sitting on the wall over our scanner and
I can send them. So long as you maintain the microdensitometer, performing
occaisional calibrations, everything is fine. (A microdensitometer without
reasonable TLC - GIGO.)

Both CCD camera's on the microscope and scanners have point-spread
functions, and these can be important. For instance, we have a rotating
drum scanner so the PSF is different along the rotation direction versus
at right angles to it. I do not know what the PSF's for modern scanners
are like - hence the question I raised a little time ago (but nobody had
more information). You also have to have adequate sampling and similar
"stuff" under control otherwise aliasing kills you.

A 2kx2k CCD is certainly competitive with film, depending upon the
application. Scratches and damage from high intensities are
problems for CCD's, but having an image immediately available so
you can look at the power-spectrum is an advantage. However, I think the
price of the TEM units has to come down before film and $2k (or even
$20k) scanners become obsolete.

On Wed, 18 Apr 2001, Dr. Edgar Voelkl wrote:

} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
}
} Sorry too :)
}
} however I feel quite confident that I can stand behind my
} experimental results. And I can assure you that film developing was
} not pushed. In addition, when digitizing film, you get a second
} non-linear function involved. Right? You can check this by using a
} Kodak photographic step tablet no.3. They are available off the
} shelf (at least used to be) and are calibrated. Density range is 21
} steps from 0.05 to 3.05. But, maybe your microdensitometer is
} already corrected for the transmittivity curve (Pixel value P = a
} 10**S, where a corresponds to the illumination intensity and S is the
} density of the film) ?
}
} Maybe, we are just talking degrees of linearity here? So, whatever
} you have may be sufficient in some cases, but no in others?
}
} Edgar
}
}
}
} }
} } Sorry,
} } Tony is correct about the linearity of film, at least after
} } digitization. We have done this test many times, and provided the film
} } is not grossly overexposed, the average exposure time and digitized
} } counts are a straight line. (There is a small offset which you can
} } correct for and is probably associated with our microdensitometer
} } electronics rather than real.)
} } Of course, if you push the film developing, you probably lost
} } the linearity.
} }
} }
} } On Wed, 18 Apr 2001, Dr. Edgar Voelkl wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Tony Garratt-Reed,
} } }
} } } your argument about film density being linear with exposure for
} } } electrons came somewhat as a surprise. As you said, a single
} } } electron will *COMPLETELY* expose many grains. So, if the next
} } } electrons hits close to that area, some of the grains that would
} } } normally become developable are already completely exposed, thus the
} } } density of the film can not double and thus the response of the film
} } } is not linear.
} } }
} } } There is a very simple test for the linearity of film if you have
} } } access to a field emission TEM with a biprism, or if you have a clean
} } } two beam case for high resolution. If you do a Fourier transform of
} } } just a cos-pattern, you should see two peaks in the Fourier
} } } transform. You can look this up in any handbook on Fourier
} } } transforms. If you do the same with a cos-pattern recorded on film,
} } } you will see plenty of higher order peaks in Fourier space. If you
} } } do this with a CCD camera, you will almost have to saturate the
} } } pixels to detect higher order terms. CCD cameras are, contrary to
} } } film, extremely linear.
} } }
} } } If you have access to a film with a linear response, I would be eager
} } } to learn about it. Otherwise, for more details on my arguments, I
} } } would point to "Density Correction of Photographic Material ....",
} } } Ultramicroscopy, 55 (1994) 75-90. It gives examples of several films
} } } tested, the equations describing the behavior and tips how to
} } } linearize the data from film with software once the data are
} } } digitized.
} } }
} } } With best regards,
} } }
} } } Edgar Voelkl
} } }
} } }
} } }
} } }
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Colleagues-
} } } }
} } } } I'm going out on a limb here, because it is not a particular area of
} } } } expertise of mine, but I've not seen it in the discussion. Experts in
} } } } microscopy of radiation sensitive materials are much more familiar with
} } } } these issues (Linn Hobbs - can you help us here?)
} } } }
} } } } I'm surprised that no-one has pointed out a very important difference
} } } } between exposure of films to photons and electrons. Film exposed to
} } } } photons exhibits a logarithmic response - that is, D varies as
} } } } log(exposure). For electrons, this is not the case, but instead, the
} } } } density is linear with exposure. This arises because individual grains
} } } } require exposure to many photons to make them "developable", whereas a
} } } } single electron will *COMPLETELY* expose many grains. For brevity, I will
} } } } not explain how this changes the exposure characteristics, but it does.
} } } }
} } } } A second consequence of this difference is that while change of development
} } } } can change the threshold exposure for development of grains sensitised by
} } } } light, grains exposed to electrons are either fully sensitized or are
} } } } completely virgin, and there is *FAR* less scope to change the image
} } } } characteristics by changing development of electron-exposed emulsions
} } } } (though there is some, for Kodak SO163, for example).
} } } }
} } } } This makes correct exposure much more critical for electron images, and
} } } } makes them much more prone to overexposure. With light, and area with D of
} } } } 4.0 has received 10,000 times more light than an area with D of 1.0. In an
} } } } electron image the area with D of 4.0 has received 4 times more electrons
} } } } than the area with D of 1.0. Do negatives really have D's above 4?
} } } } Certainly they can when exposed to electrons.
} } } }
} } } } There are other consequences. For example, the contrast (as we usually
} } } } define it as the difference in density between different areas), which is
} } } } independant of exposure on the linear portion of a film's response for
} } } } light (as explained by Gary Gaugler), is, in the case of electrons, a
} } } } linear function of the exposure. Underexposure leads to loss of contrast.
} } } } This is why images of radiation-sensitive materials are taken at low
} } } } magnification - it is the only way to maintain enough exposure of the
} } } } emulsion to give acceptable contrast, without increasing the electron dose
} } } } on the sample. Incidentally, the limit on information in such images is
} } } } probably not the "grain size" of the emulsion, but the shot noise due to
} } } } the finite number of electrons used to generate the image.
} } } }
} } } } There is much more to this topic - and I have simplified what I have said.
} } } }
} } } } Tony
} } } }
} } } }
} } } }
} } } } * * * * * * * * * * * * * * * * * * * * * * * * * *
} } } } * Anthony J. Garratt-Reed M.A., D.Phil.
} } } } * MIT, Room 13-1027
} } } } * 77 Massachusetts Avenue
} } } } * Cambridge, MA 02139-4307
} } } } * USA
} } } } * Phone: (617) 253-4622
} } } } * Fax: (617) 258-6478
} } } } *
} } }
} } } --
} } }
} } }
} } } ________________
} } } Dr. Edgar Voelkl
} } } Senior Development Staff Member
} } } ORNL
} } } Bldg 4515, MS 6064
} } } 1 Bethel Valley Road
} } } P.O. Box 2008
} } } Oak Ridge, TN 37831-6064
} } }
} } } Tel.: (865) 574-8181
} } } Fax: (865) 574-4913
} } } email: vog-at-ornl.gov
} } }
} } }
} }
} } -------------------------------------------------------
} } Laurence Marks
} } Department of Materials Science and Engineering &
} } Center for Transportation Nanotechnology
} } Northwestern University
} } Tel: (847) 491-3996 Fax: (847) 491-7820
} } mailto:ldm-at-risc4.numis.nwu.edu
} } http://www.numis.nwu.edu http://www.ctn.northwestern.edu
} } -------------------------------------------------------
} } The Other Nanotubes http://focus.aps.org/open/st12.html
} } Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html
} }
} } Workshop May 17-19 2001 "New approaches to the Phase Problem"
} } http://xraysweb.lbl.gov/esg/phasing/index.html
}
} --
}
}
} ________________
} Dr. Edgar Voelkl
} Senior Development Staff Member
} ORNL
} Bldg 4515, MS 6064
} 1 Bethel Valley Road
} P.O. Box 2008
} Oak Ridge, TN 37831-6064
}
} Tel.: (865) 574-8181
} Fax: (865) 574-4913
} email: vog-at-ornl.gov
}
}

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Wed Apr 18 13:59:47 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Wed, 18 Apr 2001 14:55:17 -0400
Subject: Exposure of film to electrons.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed Voelkl is a good microscopist whom I like and respect but he is
wrong. Of course, all detectors saturate if the signal is large
enough. But the point is how does it behave in the useful detection
range. When film is exposed to electrons the density increases linearly
with dose - until saturation effects begin. By contrast when exposed
to light, film is not linear anywhere.

If you want to get this right, please read:

The response of photographic emulsions to electrons
by R C Valentine
in Advances in Optical and Electron Microscopy, volume 1
Edited by R Barer and V E Cosslett
Academic Press

This is one of those definitive and good articles - which is also clear
and easy to read.

Alwyn Eades
--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Wed Apr 18 14:28:54 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Apr 2001 15:24:27 -0400
Subject: RE: Exposure of film to electrons.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey, I am enjoying this discussion. Any chance to get a lunchtime debate going at the M&M meeting like we had a few years ago?


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Alwyn Eades [mailto:jae5-at-lehigh.edu]
} Sent: Wednesday, April 18, 2001 2:55 PM
} To: EMNET
} Subject: Exposure of film to electrons.
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Ed Voelkl is a good microscopist whom I like and respect but he is
} wrong. Of course, all detectors saturate if the signal is large
} enough. But the point is how does it behave in the useful detection
} range. When film is exposed to electrons the density
} increases linearly
} with dose - until saturation effects begin. By contrast when exposed
} to light, film is not linear anywhere.
}
} If you want to get this right, please read:
}
} The response of photographic emulsions to electrons
} by R C Valentine
} in Advances in Optical and Electron Microscopy, volume 1
} Edited by R Barer and V E Cosslett
} Academic Press
}
} This is one of those definitive and good articles - which is
} also clear
} and easy to read.
}
} Alwyn Eades
} --
} ..........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
}


From daemon Wed Apr 18 14:45:49 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 18 Apr 2001 15:45:17 -0400
Subject: NESM Woods Hole Mtg.- May 11-12th Update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


CORRECTION!

Mary McCann's email address is: mccanns-at-tiac.net. I'm sorry for any
inconvenience to those people who have tried, unsuccessfully, to
reach her!

If anyone is interested in attending the meeting--you can email me
directly and I can send you a newsletter or fax you the information
re: program and registration.

Please include a complete mailing address and fax number.

Thanks!
Peggy Sherwood
Corresponding Secretary, NESM
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed Apr 18 15:29:20 2001



From: Fred.Shaapur-at-SEMATECH.Org
Date: Wed, 18 Apr 2001 15:25:26 -0500
Subject: Pelco DSP9 DRy Silver Processor: To be given away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If interested, please contact me directly (off-line) for further details.

Fred Shaapur, Ph.D.
Sr. Materials Analyst
International SEMATECH



From daemon Wed Apr 18 16:05:44 2001



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Wed, 18 Apr 2001 16:58:28 -0400
Subject: Re: Exposure of film to electrons.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Alwyn,

how about:

H. Frieser and E. Klein, Z. Angew. Phys. 10 (1958) 337.

H. Frieser, E. Klein and E. Zeitler, Z. Angew. Phys. 11 (1959) 190.

E. Zeitler, Ultramicroscopy 46 (1992) 405.

E. Voelkl, F. Lenz, Q. Fu and H. Lichte, "Density correction of
photographic material for further image processing ....", UM 55
(1994) 75-89.

against:

R C Valentine, in Advances in Optical and Electron Microscopy, volume 1
Edited by R Barer and V E Cosslett, Academic Press

that's 4:1 :)


Edgar

P.S.:
So far, the argument remains: a single electron "A" will completely
expose many grains. So, if the next electron "B" hits close to the
same area, some of the grains are already completely exposed through
electron "A", thus less grains become fully developed with electron
"B". Therefore, the density of the film can not double and thus the
response of the film is non-linear.




At 2:55 PM -0400 4/18/01, Alwyn Eades wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--


________________
Dr. Edgar Voelkl
Senior Development Staff Member
ORNL
Bldg 4515, MS 6064
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov


From daemon Wed Apr 18 16:05:47 2001



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Wed, 18 Apr 2001 16:26:23 -0400
Subject: RE: Exposure of film to electrons.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

Since this seems to be a lively thread, I would like to make the
following suggestion:

Most of us have an electron microscope, and I would like to suggest
the following experiment to test if your film is linear or not. It
is a simple, two-step test.

1) Take a high resolution image of some periodic structure such that
only two beams contribute to the image, preferably with identical
intensity. This will provide a cos- type imprint on your film
(actually a P+cos(...) type imprint with P} 1).

2) Develop the film and look through it at a small light source,
e.g., a halogen light. If necessary, tilt the film a little to
decrease fringe spacing for better separation of the diffraction
orders.

If you have a true linear film, no higher diffraction orders will be
visible. The two diffracted beams will be quite colorful like a
rainbow. If you see more than two diffracted beams, your film has a
non-linear response (all of those I have ever seen do (disclaimer:
the last time I have dealt with film was 1992)).


BTW,

I like Scotts suggestion of a lunchtime debate going at the M&M
meeting. Monday is out with me, but any other day is fine.


Edgar






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--


________________
Dr. Edgar Voelkl
Senior Development Staff Member
ORNL
Bldg 4515, MS 6064
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov


From daemon Wed Apr 18 18:26:14 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 18 Apr 2001 16:25:03 -0700
Subject: Re: West Coast Amray Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amray has a list of third party repair outfits. I got it from
Amray. I can fax it to you or call Amray and get it direct.

gary g.


At 11:20 AM 4/17/2001, you wrote:

} Does anyone know of service labs on the West coast for Amray
} instruments? I would appreciate knowing if any exists.
} Thank you.
}
} Franklin Bailey
} University of Idaho
} Moscow, ID 83844-2204
} jfb-at-uidaho.edu



From daemon Wed Apr 18 19:40:53 2001



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Thu, 19 Apr 2001 09:35:20 +0900
Subject: Re: Film Processing and dynamic range

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Quick and dirty way to check the linearity of the whole acquisition process
(film + scanner) is to take multiple exposures on a film by gradually
exposing its area. I've done this by using the selected area aperture. First
position the aperture so that only a small stripe of the film to be exposed.
Make an exposure. Then move the aperture to open a larger area (next
stripe). Expose ... Use a relatively low dose so that after 20 or 30 stripes
the film to be exposed to twice the dose you use in your experiments. After
scanning the film make a "line projection". Plotting the density vs. stripe
number (dose) will show you the linearity region of your acquisition system
(film + scanner).
A quick way to check the Modulation Transfer Function (MTF) of the
acquisition system is to take an exposure on the film without specimen (beam
only). Theoretically the Fourier transform of the scan should show a
constant amplitude over all spatial frequencies (Shot noise = white noise).
Average rotationally the amplitude of the Fourier transform - this is the
MTF.

Best regards,

Rado



From daemon Wed Apr 18 23:44:45 2001



From: RCHIOVETTI-at-aol.com
Date: Thu, 19 Apr 2001 00:39:03 EDT
Subject: Digital SEM Imaging?

Contents Retrieved from Microscopy Listserver Archives
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Fellow Listmembers,

Does anyone know of a digital imaging system for scanning EM? I know that
CCD imaging systems for TEMs have been discussed in the group before, but I
don't recall whether SEMs have been discussed. Perhaps someone could tell me
if this subject has been archived, or perhaps someone has a summary. Vendors
should also feel free to respond directly to me off-list.

Someone has asked me for information on a digital image acquisition system
for SEM (they currently have a screen with a Polaroid camera attachment). If
anyone has recommendations for such a digital acquisition system, please
contact me with the details.

Thanks very much in advance.

Bob Chiovetti
GTI Microsystems, Inc.
Leica Exclusive Regional Dealer
Southwestern United States
rchiovetti-at-aol.com


From daemon Thu Apr 19 06:06:03 2001



From: Mark Auty :      mauty-at-MOOREPARK.TEAGASC.IE
Date: Thu, 19 Apr 2001 12:01:55 +0100
Subject: food microscopy short courses

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues

Just let you know that there will be two short courses on food
microscopy in Minneapolis on May 13 as part of the Food
Structure & Functionality Symposium.

Course 1 is aimed at researchers interested in specific localisation
techniques as a tool for understanding structure-function
relationships in
foods. Course 2 gives a grounding in light microscopy technques
(optical
contrast, staining) used for the identification of food materials and
contaminants (glass, fibres, plastics etc) and is aimed at quality
control/research & development personnel.

Details can be found at:
http://www.aocs.org/meetings/am2001/foodstr.htm

Both courses offer practical tuition by internationally recognised
speakers.

Let me know if you are interested, or you can book online at:
https://www.aocs.org/meetings/am2001/regshort.htm

Regards
Mark

Mark Auty
Dairy Products Research Centre
Moorepark
Fermoy
Co. Cork
Ireland

tel +353 25 42447
fax +353 25 42340
mauty-at-moorepark.teagasc.ie




From daemon Thu Apr 19 06:54:54 2001



From: Hayes, Fred :      FHayes-at-TAC.Textron.com
Date: Thu, 19 Apr 2001 07:51:02 -0400
Subject: LKB Knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking to buy a working LKB glass knifemaker.

Fred Hayes
FHayes-at-TAC.Textron.com


From daemon Thu Apr 19 07:33:56 2001



From: Mark Auty :      mauty-at-MOOREPARK.TEAGASC.IE
Date: Thu, 19 Apr 2001 07:31:25 -0500
Subject: Food microscopy short courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

Just let you know that there will be two short courses on food
microscopy in Minneapolis on May 13 as part of the Food
Structure & Functionality Symposium.

Course 1 is aimed at researchers interested in specific localisation
techniques as a tool for understanding structure-function
relationships in foods.
Course 2 gives a grounding in light microscopy technques (optical
contrast, staining) used for the identification of food materials and
contaminants (glass, fibres, plastics etc) and is aimed at quality
control/research & development personnel.

Details can be found at:
http://www.aocs.org/meetings/am2001/foodstr.htm

Both courses offer practical tuition by internationally recognised
speakers.

Let me know if you are interested, or you can book online at:
https://www.aocs.org/meetings/am2001/regshort.htm

Regards
Mark

Mark Auty
Dairy Products Research Centre
Moorepark
Fermoy
Co. Cork
Ireland

tel +353 25 42447
fax +353 25 42340
mauty-at-moorepark.teagasc.ie


From daemon Thu Apr 19 07:59:18 2001



From: tbargar-at-unmc.edu
Date: Thu, 19 Apr 2001 07:53:41 -0500
Subject: want to contact JEOL-220A SEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I would like to get in contact with anyone currently using a JEOL-220A SEM.
I particularly want to know if it is possible to add a digital image
acquisition system to this SEM. Our lab has a chance to acquire a 220A
which has less than 200 hours of use and was under service contract before
going into storage 4 years ago. Any and all information would be
appreciated. Thanks.

Tom Bargar
EM Lab
U. Neb. Med. Ctr.
phone (402)-559-7347
tbargar-at-unmc.edu



From daemon Thu Apr 19 08:27:51 2001



From: Dr. Klaus Jandt :      K.Jandt-at-bristol.ac.uk
Date: Thu, 19 Apr 2001 14:23:25 +0100
Subject: Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Biomaterials Science Group
Department of Oral and Dental Science
University of Bristol
in collaboration with Glaxo SmithKline

Postdoctoral Research Assistant

in the Biomaterials Science Group
on the project
Interaction Mechanisms of Polymers at Interfaces of Mineralised Tissues


The research area involves the study of the physical and chemical properties
and interaction mechanisms of different polymers at interfaces of
mineralised tissues. You will have recently been awarded a PhD in an
appropriate field and will ideally have experience in scanning probe
microscopy (AFM) of biological materials and other analytical techniques and
an interest in medical research. You will work in Dr. Jandts group and
interact with scientists at Glaxo Smith Kline.
The University of Bristol is one of the leading research universities in the
UK and provides an outstanding scientific training environment to enhance
your qualification. The group is involved in exciting, interdisciplinary
projects and maintains appropriate state of the art instrumentation. There
exist opportunities for additional interactions with clinical scientists and
other centres at the university.
We are looking for a dynamic and exceptionally well-qualified postdoctoral
researcher who can interact effectively in an international and
interdisciplinary team. The appointment will be on a Research Assistant 1A
scale with a salary range of # 16775 to # 20465. This is a full time
appointment and initially for one year. Applicants should include a short
CV, stating research experience and interests, publication list and
addresses of two referees. The review of applications will start 24 May 2001
and will continue until the post has been filled.
Informal inquiries can be directed by email to Dr. K. D. Jandt
(K.Jandt-at-bris.ac.uk), Senior Lecturer in Biomaterials, University of Bristol

Formal applications quoting the reference number 7401 should be directed to

The University of Bristol
Recruitment Office
Bristol, BS8 1TH
United Kingdom



-----------------------------------------------------------------
Dr. rer. nat. Klaus D. Jandt
Senior Lecturer in Dental Materials Science and Biomaterials
University of Bristol, Department of Oral and Dental Science
Lower Maudlin Street, Bristol, BS1 2LY, UK
Phone: +44-117-9284418, Fax: ++44-117-9284780
Internet: K.Jandt-at-bris.ac.uk
WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm
"We make Biomaterials Science work!"



From daemon Thu Apr 19 11:32:11 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Thu, 19 Apr 2001 12:26:40 -0400
Subject: Ionization gauge cabling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have managed to get a Varian 842 ionization gauge controller and am
working on the tube itself, leaving only one component - the cable(s) -
remaining. Varian wants $190 for this cable, which seems a little steep to
me.

} From the connections on the controller box it looks like there is a four
wire cable with an octal plug going that would go to some connector on the
bottom of the tube and a separate coax connection for the collector. Are the
connectors that would attach to the bottom and top of the tube readily
available components and, if so, where could I buy them? Is there some place
where I could simply buy the entire cable system at a better price than what
Varian is asking? What is so special about five wires and a couple of
connectors that warrants that kind of price?

I would appreciate information from anyone regarding the connection of ion
gauge tubes to their controllers, Varian or not.

Bruce Girrell



From daemon Thu Apr 19 14:09:39 2001



From: craig.bennett-at-acadiau.ca
Date: Thu, 19 Apr 2001 16:02:35 -0300
Subject: Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ASSISTANT DIRECTOR (TECHNICAL)
Acadia Centre for Microstructural Analysis (ACMA)
Competition #01-17

The Acadia Centre for Microstructural Analysis (ACMA) has
recently been established to support research initiatives
within the Faculty of Pure & Applied Science and provide
technical services to the regional R&D community. We are
seeking an individual to manage and maintain the
microanalytical facility consisting of scanning and
transmission electron microscopes, scanning probe
microscope, FTIR spectrometer, epi-fluorescence and
confocal microscopes along with associated specimen
preparation equipment.

Responsibilities: management of day to day operations
including training and general supervision of users,
technical support and general maintenance of research
equipment, technical service and consulting activities,
marketing of technical services.

Qualifications: A minimum of a Masters Degree in natural
sciences/engineering with courses in computer
programming and electronics or an equivalent combination
of education and related work experience; experience in
instrumentation; some expertise with one or more of TEM,
SEM, FTIR, SPM or confocal microscopy; strong
communication, writing and interpersonal skills. An ability to
provide general electronics and instrumentation support to
the Faculty would be considered an asset.

The Assistant Director will be a full-time employee of Acadia
University and the initial appointment shall be for one year,
with the possibility of renewal for up to five years.

Salary Range: $39,615 to $51,645 per annum, depending
on qualifications

Closing Date for Applications: June 1, 2001

Please send your resume including three letters of
references to:
Marian Reid, Personnel Officer
Acadia University, Wolfville NS B0P 1X0
E-mail: marian.reid-at-acadiau.ca
Fax: 902-585-1075

We thank all applicants in advance but advise that only those
selected for an interview will be contacted. Acadia University
reserves the right not to fill this position.

In accordance with Canadian immigration requirements, this
advertisement is directed to Canadian citizens and permanent
residents.

An equal opportunity employer, Acadia welcomes applications from
qualified women and men, including African Nova Scotians, First
Nations peoples, persons with disabilities, and racially visible
people. Such individuals are encouraged to self-identify.

Dr. Craig Bennett
Associate Professor and Acting Head
Department of Physics, Acadia University
and
Director, Acadia Centre for Microstructural Analysis
Wolfville, Nova Scotia, Canada B0P 1X0
tel. 902-585-1150 fax. 902-585-1816


From daemon Thu Apr 19 14:31:29 2001



From: Karli Fitzelle :      fitzelle.1-at-osu.edu
Date: Thu, 19 Apr 2001 15:27:05 -0400
Subject: TEM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all!
I have a quick question. I am working with purified nuclear matrix samples
and need tips for the fixation / embedding process. First of all, the
samples are so small, they are nearly invisible, and secondly, they are
incredibly fragile, so great care must be taken during this whole process.
To complicate matters, we are doing pre-embedding immunolabeling. The
problem comes in after incubation in secondary (gold) antibody. We have to
spin down the sample after each rinse or incubation. My concern is about
the gold particles........At what RPM will they sediment? Has anyone ever
encountered this dileama before? Likewise, does anyone have any tips for
fixation and embedding of this kind of sample?
Question # 2: Is it ok to go straight from Prop Ox to 100% resin
(spurr's)? The matrix is mostly protein, so it shouldn't take long at all
to infiltrate...but I am unsure about how to go about infiltrating. In the
viscous resin, we won't be able to spin down the matrix, that's where the
problem arises.

Any help would be greatly appreciated!

Thanks in advance,
Karli
Karli Fitzelle
MCI Center
OARDC / OSU
Wooster, Ohio 44691
330-263-3828


From daemon Thu Apr 19 14:39:23 2001



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Thu, 19 Apr 2001 13:35:43 -0600
Subject: TEM: Determination of section thickness

Contents Retrieved from Microscopy Listserver Archives
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I have a user in our EM Center who would like to have a procedure for
determining the thickness of their ultrathin sections, 60-100 nm. They are
using the normal color criterion of "silver" sections in the boat to select
their sections, but would like to be more precise. There is a potential for
doing morphometry and comparisons of particle counts between non-serial
sections and sections from different specimens.

They have heard of a technique that uses small particles applied to both
surfaces of the section and using tilt and geometry of the TEM stage to
determine section thickness. Any details of this technique would be
appreciated.

All suggestions are welcome.

John
Colorado State University
john.chandler-at-colostate.edu



From daemon Thu Apr 19 15:36:45 2001



From: Paul Anderson :      paanders-at-lynx.dac.neu.edu
Date: Thu, 19 Apr 2001 16:31:48 -0400 (EDT)
Subject: JEOL hot/bulk holders

Contents Retrieved from Microscopy Listserver Archives
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Sample holders for JEOL 100CX-2000EXII model TEMs for sale:

Gatan hot stage sample holders, air/water cooled. Both furnaces in
working order; holders include one hexnut lockring AND thermocouple
controller (sorry, no power wires--lost!):
--#1 in good condition, $12,000 (Gatan Power supply model #580-0300)
--#2 lightly stripped hex nut assembly in furnace (doesn't tighten all
the way but sample will remain in place fairly well),
mA display on power supply (Gatan power supply model #628-0500)
a bit jumpy but thermocouple reads temperature fine, $7000

Extra washers (Gatan part #628-0223), and hex nut tool (Gatan part
#608-0005) NOT included with the above holders.

JEOL EM-SCSH
--Common bulk specimen holder (STEM) with graphite retainer, $1000

Please contact Terry K. Baker for further inquiries and negotiations:
Phone 508 893 9560
baker-at-catalytic-materials.com
Catalytic Materials
1750 Washington St.
West Holliston Professional Park, Suite C2
Holliston, MA 01746



From daemon Thu Apr 19 16:03:04 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 19 Apr 2001 16:58:47 -0400
Subject: Re: TEM Question

Contents Retrieved from Microscopy Listserver Archives
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I would try to immobilize the stuff on some substrate that could then be
carried like a piece of tissue. I am not sure what that would be in this
case. Possibly a Nuclepore filter???

At 03:27 PM 4/19/2001 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Greg Erdos
Assistant Director
Biotechnology Program Ph. 352-392-1295
University of Florida Fax 352-846-0251
PO Box 118525
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Thu Apr 19 18:48:47 2001



From: Raymond Bennett :      rbennett-at-hortresearch.co.nz
Date: Fri, 20 Apr 2001 11:38:40 +1300
Subject: Selective Staining of Xylem tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello;

I have been asked by one of our collegues hereas to wether there
is a selective stain for viewing xylem tissue that has been
embedded in resin.
These are 1-7um sections viewed with a light microscope

Somewhere in the deep dark recesses (which are not accessible on
a Friday afternoon or Monday morning ) I seem to remember there
is one; or indeed, a publication that lists many of these such
permeations.

Any help appreciated
Cheers

Raymond Bennett
Keith Williamson EM Unit
Hort+Research
Palmerston North
NEW ZEALAND


_________________________________________________________________
The contents of this e-mail are privileged and/or confidential to the named
recipient and are not to be used by any other person and/or organisation.
If you have received this e-mail in error, please notify the sender and delete
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_________________________________________________________________


From daemon Thu Apr 19 19:31:44 2001



From: Tom Murray :      tm8a-at-virginia.edu
Date: Thu, 19 Apr 2001 20:30:10 -0400
Subject: Re: Ionization gauge cabling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I have managed to get a Varian 842 ionization gauge controller and am
} working on the tube itself, leaving only one component - the cable(s) -
} remaining. Varian wants $190 for this cable, which seems a little steep to
} me.
}
} } From the connections on the controller box it looks like there is a four
} wire cable with an octal plug going that would go to some connector on the
} bottom of the tube and a separate coax connection for the collector. Are the
} connectors that would attach to the bottom and top of the tube readily
} available components and, if so, where could I buy them? Is there some place
} where I could simply buy the entire cable system at a better price than what
} Varian is asking? What is so special about five wires and a couple of
} connectors that warrants that kind of price?
}
} I would appreciate information from anyone regarding the connection of ion
} gauge tubes to their controllers, Varian or not.
}
} Bruce Girrell


I got a similar cable from Duniway Stockroom. They were much cheaper
than Varian, and the cable has worked great.

Tom


--
Thomas Mullarkey Murray email:tm8a-at-virginia.edu
Thornton Hall - MSE phone:(804)982-5659
University of Virginia Fax: (804)982-5660
Charlottesville, VA 22903


From daemon Thu Apr 19 19:35:35 2001



From: karenco-at-discoverymail.com ()
Date: Thu, 19 Apr 2001 19:43:24 -0500
Subject: Ask-A-Microscopist: immunolocalization question LM/TEM/SEM

Contents Retrieved from Microscopy Listserver Archives
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Try "Duniway Stockroom" at www.duniway.com

or (800) 446-8811

Regards,

Earl
----- Original Message -----
} From: "Bruce Girrell" {bigirrell-at-microlinetc.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, April 19, 2001 9:26 AM



Email: karenco-at-discoverymail.com
Name: karen chamusco

Organization: university of florida

Education: Graduate College

Location: gainesville, fl

Question: I am learning to do SEM now and have been doing
immunolocalization (histogold) for light microscopy. I am now
interested in applying this histogold immunolocalization in EM work.
It appears that most immunolocalization I've seen is in TEM. I am
working in SEM. Is there any advantage of one over the other besides
the obvious difference that TEM looks inside the tissue and SEM looks
at the surface? How come there aren't as many SEM localizations out
there (that I've seen)? Thank you for your time, Karen

---------------------------------------------------------------------------


From daemon Thu Apr 19 21:34:19 2001



From: EMSL Lab - Miami :      miamilab-at-emsl.com
Date: Thu, 19 Apr 2001 21:30:39 -0500
Subject: EDXA, Need help with detector geometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Subject: EDXA, Need help with detector geometry
}
}
} } Sirs or Madams,
} }
} } I am running a JEOL CX II with a Kevex mod. 3200-0018 detector/ Kevex
Delta
} } Class Anlyzer.
} }
} } I am having difficulty locating the geometric variables unique to this
} } mating of scope and detector. Kevex was unable to supply the data.
These
} } geometric variables are used by the analyzer software (Quantex) in
modeling
} } and subtracting backgrounds.
} }
} } The variables I am unable to supply are Working Distance, Fixed Distance,
} } and Azimuth. I have seen reference to a Quantex Parameters List. This
} } document was shipped with the original equipment, but alas, this is a
} second
} } hand scope and the detector was taken from the company warehouse.
} }
} } Does anybody use this combination of TEM and detector or know of someone
} } with this combination? Does anyone wish to share a document listing
} Quantex
} } parameters for different scopes with Kevex detectors?
} }
} } My sanity is in your hands.
} } I remain humbly yours,
} }
} }
} } Stephen Bennett
} } EMSL Analytical, Inc.
} } Miami, FL
} }
} } miamilab-at-emsl.com
} }


From daemon Fri Apr 20 00:27:57 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Fri, 20 Apr 2001 07:21:42 +0200
Subject: Antwort: Selective Staining of Xylem tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Raymond,

a good reference book on staining plant tissues is

Horobin, R.W., Histochemistry, G.Fischer, Stuttgart, 1982

I am sure you will find differential staining methods for xylem in there
..

Using Toluidin Blue O gives good results just by the more intense (dark
blue) xylem cell walls compared to the thinner (light blue) parenchyma cell
walls - but this is not a differential staining method!

Hope this helps you,

Joachim

Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350



From daemon Fri Apr 20 00:35:39 2001



From: Earl Weltmer :      eweltmer-at-home.com
Date: Thu, 19 Apr 2001 22:31:34 -0700
Subject: FESEM Bellows repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have a Hitachi S-800 FESEM gun bellows that has developed a small leak.
Although it has been replaced, I have heard of bellows being repaired by
plating with cadmium or some other metal.

Does anyone have any experience with this?

Thank You,

Earl Weltmer




From daemon Fri Apr 20 02:30:37 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 20 Apr 2001 08:39:46 +0100 (GMT Daylight Time)
Subject: Re: TEM: Determination of section thickness

Contents Retrieved from Microscopy Listserver Archives
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Karli
Greg is right. A nuclepore filter could be used to separate small
specimens from reagents, which could be exchanged using a syringe. But
this is only one of various options. Another option would be to
encapsulate the specimens in low-melting point agarose. Then they can
be handled in the same way as as tissue blocks. However,
centrifugation using a low-speed centrifuge such as an eppendorff
centrifuge, will easily separate the unbound gold probe from your
specimens. If you are worried about this, test a sample of you
colloidal gold probe in your centrifuge. If the supernatant stays pink
and there is no red pellet the gold is still a sol. I would recommend
that you process the tissue as you are doing, using centrifugation,
until the immunolabelling procedure is completed, and at that stage
embed the specimen pellet into agarose prior to dehydration and resin
infiltration.

Moving your specimen direct from propylene oxide to pure resin is a
recipe for specimen collapse unless your specimens are a) exceedingly
small, b) very permeable to the resin. What happens is the very mobile
PPO comes out of the specimen faster than the viscous resin can move
into it, resulting in a volume reduction and shrinkage. You can
normally get away with two or three intermediate steps and these are
much easier to accomplish if your specimens are in large pieces (we're
talking relative sizes here - large means visible, cubes maybe 0.2 to
1 mm in a side)
Good luck
Chris

----- Original Message -----
} From: "Greg Erdos" {gwe-at-biotech.ufl.edu}
To: "Karli Fitzelle" {fitzelle.1-at-osu.edu} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, April 19, 2001 9:58 PM


Hi John,

This is a simple parallax problem.

Imagine the specimen in cross section. If there are two
particles one vertically above the other they are seperated
by the film thickness T. Tilt the film through an angle A
and in plan view the particles will seperate by a distance
D. (This can also be extended to account for two particles
not vertically above each other but I'll stick to the easy
case for the explaination.)

Take two negatives one at zero tilt and one at tilt of A
and measure the seperation D, the thickness can be
calculated by T=D/sinA.

You will need to know the direction of the tilt axis for
your measurements and you can see that the larger the tilt
angle and the more acurately you can measure the seperation
the more accurate your measurement will be. Tilt + and - A
to get a more accurate result.

Now you have to think about what you are going to use to
mark the surfaces as you need to be able to distinguish the
markers at two different tilt angles. Crystals may go in
and out of contrast making them difficult to follow. If you
use spheres don't forget to subtract the diameter of the
sphere (or one the sum of the two radii) from your total
thickness to account for the fact that you are probably
using the circumferance as the marker.

Of course the alternative is to re-embed and cross section
to measure the thickness directly.

With any luck there may be some responses from people
currently measuring thicknesses using this, or other
techniques, with some relevant hints or comments on
expected accuracy of the different methods.

Good luck,
Ron

On Thu, 19 Apr 2001 13:35:43 -0600 John Chandler
{chandler-at-lamar.ColoState.EDU} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a user in our EM Center who would like to have a procedure for
} determining the thickness of their ultrathin sections, 60-100 nm. They are
} using the normal color criterion of "silver" sections in the boat to select
} their sections, but would like to be more precise. There is a potential for
} doing morphometry and comparisons of particle counts between non-serial
} sections and sections from different specimens.
}
} They have heard of a technique that uses small particles applied to both
} surfaces of the section and using tilt and geometry of the TEM stage to
} determine section thickness. Any details of this technique would be
} appreciated.
}
} All suggestions are welcome.
}
} John
} Colorado State University
} john.chandler-at-colostate.edu
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Apr 20 02:52:17 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 20 Apr 2001 08:52:44 +0100 (GMT Daylight Time)
Subject: Re: FESEM Bellows repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Earl,

We get the bellows replaced by a local (UK) firm, typically
around 250 pounds for edge welded bellows in stainless
steel.
This includes removing the old bellows, supply and welding
in new bellows and leak testing. It usually takes a few
weeks unless we are prepared to pay to interupt the work
scedule.
These prices are for 20-40mm dia 50-80mm long bellows.

There must be firms in the US (and most other places) to do
this.

Ron


On Thu, 19 Apr 2001 22:31:34 -0700 Earl Weltmer
{eweltmer-at-home.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
}
} I have a Hitachi S-800 FESEM gun bellows that has developed a small leak.
} Although it has been replaced, I have heard of bellows being repaired by
} plating with cadmium or some other metal.
}
} Does anyone have any experience with this?
}
} Thank You,
}
} Earl Weltmer
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Apr 20 02:54:55 2001



From: Raymond Grassl :      Grassl.Raymond-at-basco.com
Date: Fri, 20 Apr 2001 08:35:47 -0500
Subject: SEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
To: {karenco-at-discoverymail.com}
Sent: Friday, April 20, 2001 8:52 AM


Howdy Y'all,

I am looking for an inexpensive vise-style specimen holder for our
SEM. It does not need to be fancy, just a flat-jawed simple device
with a screw to tighten the faces of the jaws. After becoming
frustrated by all the information available on the web,I'm sure
someone out there could be of assistance. Please help by responding
online.

Regards,

Ray Grassl

Grassl.raymond-at-basco.com


From daemon Fri Apr 20 08:39:11 2001



From: EMSL Lab - Miami :      miamilab-at-emsl.com
Date: Fri, 20 Apr 2001 08:36:27 -0500
Subject: S-800 FESEM gun bellows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mr. Weltmer,
Try finding an aircraft grade, two-part epoxy (Epoxo 88). If you have
stress cracks on a vacuum bellows, mix a batch of epoxy and thinly spread
over the joints and areas where you suspect a crack. Make sure to prep the
area with acetone or methanol and wait for the solvent to flash off before
applying the epoxy. I have repaired a vacuum manifold on a Poloron plasma
asher and have not had a leak for a year now. The epoxy doesn't seem to
outgass enough to bother. You may want to place the joint in a muffle
furnace at low temp. after a few hours to make sure the product is fully
cured.

Stephen Bennett
EMSL Analytical, Inc.
Miami, Fl


From daemon Fri Apr 20 09:15:24 2001



From: greg :      greg-at-umic.sunysb.edu
Date: Fri, 20 Apr 2001 10:14:00 -0400
Subject: Re: Ask-A-Microscopist: immunolocalization question LM/TEM/SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karen,
Here is a paper that deals with immunoelectron
microscopy for the SEM. If you have any questions
you can contact me at the e-mail or phone below.

Coller, Barry S., Kutok, J. L., Scudder, L. E.,
Galanakis, D. K., West, S. M . , Rudomen, G. S.,
Springer, K. T., "Studies of Activated GPIIb/IIIa
Receptors on the Luminal Surface of Adherent
Platelets: Paradoxical Loss of Luminal Receptors
When Platelets Adhere to High Density Fibrinogen."
J. Clin. Invest. Vol. 92, pp. 2796-2806, 1993

karenco-at-discoverymail.com wrote:
}

}
} Email: karenco-at-discoverymail.com
} Name: karen chamusco
}
} Organization: university of florida
}
} Education: Graduate College
}
} Location: gainesville, fl
}
} Question: I am learning to do SEM now and have been doing
} immunolocalization (histogold) for light microscopy. I am now
} interested in applying this histogold immunolocalization in EM work.
} It appears that most immunolocalization I've seen is in TEM. I am
} working in SEM. Is there any advantage of one over the other besides
} the obvious difference that TEM looks inside the tissue and SEM looks
} at the surface? How come there aren't as many SEM localizations out
} there (that I've seen)? Thank you for your time, Karen
}
} ---------------------------------------------------------------------------

--
Regards,
Gregory Rudomen
Technical Specialist Electron Microscopy
State University of New York at Stony Brook
University Microscopy Imaging Center
Stony Brook, NY 11794-8088 W-631-444-7372
Greg-at-umic.sunysb.edu--http://www.umic.sunysb.edu
*************************************************
Standard disclaimer: The opinions expressed
in this communication are my own and do
not necessarily reflect those of the University
Microscopy Imaging Center.
*************************************************


From daemon Fri Apr 20 09:39:47 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Fri, 20 Apr 2001 10:37:28 -0400
Subject: Ionization gauge cabling - results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to this question.

The consensus clearly is Duniway Stockroom Corp. of Mountain View, CA. I
called them and they have the proper cable in stock for less than half of
what Varian wants. Your collective help is greatly appreciated.

Bruce Girrell



From daemon Fri Apr 20 09:57:08 2001



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 20 Apr 2001 09:53:39 -0500 (CDT)
Subject: Re: Selective Staining of Xylem tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Raymond,

If you first remove the plastic from your semi-thin sections, you can use
most histological stains. A saturated solution of sodium hydroxide in
methanol (sodium methoxide) will remove the plastic from 500 nm sections
of plant material in about 10 minutes. Rinse the slides in 100% methanol
followed by water and they are ready to go.

Tiny sections are sometimes very hard to find after the resin is removed,
so we usually draw around the sections that have been heat-fixed to the
slide with a glass scribe.

We (and others) have successfully used analine blue, Auramine O, and Sudan
black on Arabidopsis. It is sometimes necessary to remove osmium from the
de-plasticized sections (with sodium meta-periodate) for the staining
protocols to work.

Most of these techniques are in M.A. Hayat's Principals and Techniques of
Electron Microscopy Biological Applications.

If you'd like some references, I'll hunt them up.

Heather Owen


Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Fri Apr 20 10:06:07 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sat, 21 Apr 2001 01:04:21 +1000
Subject: RE: FESEM Bellows repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ron,
They are robbing you. For less than that price you should get a complete bellow
including fittings and freight. That way you can continue operation with the
old bellows silicone sealed until the replacment bellows are available.
Disclaimer:
ProSciTech supplies SS bellows.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, April 20, 2001 5:53 PM, Ron Doole
[SMTP:ron.doole-at-materials.oxford.ac.uk] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Earl,
}
} We get the bellows replaced by a local (UK) firm, typically
} around 250 pounds for edge welded bellows in stainless
} steel.
} This includes removing the old bellows, supply and welding
} in new bellows and leak testing. It usually takes a few
} weeks unless we are prepared to pay to interupt the work
} scedule.
} These prices are for 20-40mm dia 50-80mm long bellows.
}
} There must be firms in the US (and most other places) to do
} this.
}
} Ron
}
}
} On Thu, 19 Apr 2001 22:31:34 -0700 Earl Weltmer
} {eweltmer-at-home.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} }
} } I have a Hitachi S-800 FESEM gun bellows that has developed a small leak.
} } Although it has been replaced, I have heard of bellows being repaired by
} } plating with cadmium or some other metal.
} }
} } Does anyone have any experience with this?
} }
} } Thank You,
} }
} } Earl Weltmer
} }
} }
} }
}
} ----------------------
} Mr. R.C. Doole
} Department of Materials,
} University of Oxford.
} Parks Road, Oxford. OX1 3PH. UK.
} Phone +44 (0) 1865 273701
} Fax +44 (0) 1865 283333
} ron.doole-at-materials.ox.ac.uk
}



From daemon Fri Apr 20 10:20:53 2001



From: Paul Webster :      pwebster-at-hei.org
Date: 20 Apr 01 08:20:32 -0700
Subject: Re:TEM Question

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Karli Fitzelle writes:
I have a quick question. I am working with purified nuclear matrix samples and need tips for the fixation / embedding process.......etc

Hi Karli,
Chris Jeffree gave you the advice you need - embed the samples in low melting point agarose. Do this at the very beginning of the process after you have fixed them but washed away the fixative. You can then cut the embedded pieces into small blocks and do all your immunolabeling on these blocks. The agarose is permeable to antibodies and colloidal gold. You may need to increase your incubation and washing times to reflect the reduced accessibility. You will also not have to worry about centrifugation or rushing through the resin embedding as the sample will essentially behave as small tissue pieces.

If you do centrifuge the samples then do not worry about losing the gold - it will remain attached to the antibodies. Centrifuging the gold prior to labeling is another matter.

Regards,

Paul Webster


Paul Webster, Ph.D.
Scientist II & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Apr 20 10:22:09 2001



From: Glenn Fried :      gfried-at-uiuc.edu
Date: Fri, 20 Apr 2001 10:20:44 -0500
Subject: lm: light microscopy position opening

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X-Mailer: QUALCOMM Windows Eudora Version 4.3.1


The Imaging Technology Group at the Beckman Institute, University of
Illinois at Urbana-Champaign, is seeking a light microscopist to work in
our multi-user Microscopy Suite.

Responsibilities will include:

· Operating/maintaining the confocal microscope, fluorescence
microscope, stereology workstation, and stereo dissecting microscope.
· Supervising and training others in the use of these instruments.
· Working in conjunction with users to apply light microscopy
techniques to their research.
· Developing novel applications to take advantage of the unique
capabilities of this instrumentation.

A complete job description is posted on the ITG web page
http://www.itg.uiuc.edu
or http://www.itg.uiuc.edu/ms/lightmicroscopistjob.pdf

Please send a letter of application and resume to:

Lori Heil
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
405 N. Mathews
Urbana, IL 61801
(217) 244-0170
e-mail: lheil-at-uiuc.edu

The University of Illinois is an Affirmative Action/Equal Opportunity
Employer. Women and minorities are encouraged to apply.



From daemon Fri Apr 20 11:03:58 2001



From: simkin-at-egr.msu.edu
Date: Fri, 20 Apr 2001 11:58:53 -0400 (EDT)
Subject: RE: SEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ray,
I have made a number of specimen holders over the last few years, at rock-
bottom prices :). My recipe for a pin-mount style mini-vice is:
- grind or cut a small block of brass to the vice size desired
- drill one hole in the large face of the block the size of the pin (typ. 3mm)
- drill two parallel holes (the size for tap holes) through the long
axis of the block
- cut off one end of the block perpendicular to the two tap holes
- tap the holes in the larger section of the block
- drill out the tap holes in the smaller section of the block to make
clearance holes
- cut off a length of brass rod for the pin mount, and solder it in place
in the hole in the larger block
- screw two bolts (brass recommended) into the large block through the
clearance holes in the smaller block. This is your mini-vice.
- de-burr, clean, and polish as desired.

With proper tools several of these can be made in an hour; with what's available from the local hobby store, maybe 1.5-2 hours each.

Ben (simkin-at-egr.msu.edu)

} Howdy Y'all,
}
} I am looking for an inexpensive vise-style specimen holder for our
} SEM. It does not need to be fancy, just a flat-jawed simple device
} with a screw to tighten the faces of the jaws. After becoming
} frustrated by all the information available on the web,I'm sure
} someone out there could be of assistance. Please help by responding
} online.
}
} Regards,
}
} Ray Grassl
}
} Grassl.raymond-at-basco.com



From daemon Fri Apr 20 11:09:57 2001



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Fri, 20 Apr 2001 11:06:25 -0500
Subject: Re: TEM: Determination of section thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} }
} }
} } I have a user in our EM Center who would like to have a procedure for
} } determining the thickness of their ultrathin sections, 60-100 nm. They are
} } using the normal color criterion of "silver" sections in the boat to select
} } their sections, but would like to be more precise. There is a potential
} } for
} } doing morphometry and comparisons of particle counts between non-serial
} } sections and sections from different specimens.
} }
} } They have heard of a technique that uses small particles applied to both
} } surfaces of the section and using tilt and geometry of the TEM stage to
} } determine section thickness. Any details of this technique would be
} } appreciated.
} }
} } All suggestions are welcome.
} }
} } John
} } Colorado State University
} } john.chandler-at-colostate.edu
} }


The is a very good review of measuring section thickness in Audrey Glauert's
book "Sectioning and Cryosectioning for Electron Microscopy". Several
different methods are discussed. In short, it is difficult to know precisely
how thick a silver section is. My edition of the book was published in 1991.
There may be newer and more encouraging reports that I am not aware of.

Good Luck,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Fri Apr 20 11:10:55 2001



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 20 Apr 2001 11:07:28 -0500
Subject: Re: tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karli, have you considered using magnetic beads to carry your organelles
through these steps? The process is less disruptive to fragile organelles
than centrifugation. Whether the gold will settle during centrifugation
will depend on the size of the gold, an example is 15 nm. gold settles at
25,000 rpm for 60 minutes. For a discussion of this issue, you can consult
the early papers describing the conjugation of various proteins to colloidal
gold,
Marge

Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation
email: springett.margaret-at-mayo.edu


} ----------
} From: Karli Fitzelle
} Sent: Thursday, April 19, 2001 2:27 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM Question



From daemon Fri Apr 20 11:16:16 2001



From: NPGSlithography-at-aol.com
Date: Fri, 20 Apr 2001 12:12:41 EDT
Subject: Re: Digital SEM Imaging?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 4/19/2001 3:01:22 AM Mountain Daylight Time,
RCHIOVETTI-at-aol.com-at-sparc5.microscopy.com writes:

} Does anyone know of a digital imaging system for scanning EM?

A list of companies that provide digital imaging systems for SEMs can be
found at
"www.jcnabity.com/links.htm#Digital Imaging".

The two basic types of systems are passive (where they acquire an image while
the SEM scans) and active (where they control the beam position during the
image acquisition). The passive types will typically be less expensive,
since they do not need to generate the sweep voltages. The active systems
require that the SEM has XY inputs for beam control.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Fri Apr 20 12:52:59 2001



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Fri, 20 Apr 2001 13:46:17 -0400
Subject: Thin sections and glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I teach an undergraduate-level course in electron microscopy, and
every year I find that the biggest hurdle for my students, not
surprisingly, is the cutting of thin sections with glass knives.
This is of course the point at which the students begin to get very
discouraged with their individual research projects. I have tried
various embedding media based on viscosity/penetration demands
(Spurr's or ultra-low viscosity for plant tissue, for example), but I
have been unable to come up with a medium that gives students a
greater chance at success in cutting sections with glass knives.
Does anyone have a favorite embedding medium that would allow fairly
routine sectioning of diverse biological samples with glass knives.
We are using primarily MT-2 microtomes.

I thank you in advance.

Dick Briggs
Biology Department
Smith College


From daemon Fri Apr 20 13:44:57 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 20 Apr 2001 14:38:26 -0500
Subject: vacuum leak repairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Earl Weltmer wrote:
=============================================
I have a Hitachi S-800 FESEM gun bellows that has developed a small leak.
Although it has been replaced, I have heard of bellows being repaired by
plating with cadmium or some other metal.
==============================================
Is this one of those kinds of leaks that could be repaired, at least
temporarily, with a product like VacSeal™ vacuum leak sealant? See URL
http://www.2spi.com/catalog/vac/vacleak.html

It is used even on UHV systems for "temporary" repairs, but depending on the
nature of the leak, sometimes these "temporary" repairs can last months or
even years.

And this kind of fix is quick and cheap!

Disclaimer: SPI Supplies offers this product to those with vacuum leaks.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Fri Apr 20 13:45:21 2001



From: rgriffin-at-eng.uab.edu
Date: Fri, 20 Apr 2001 13:41:44 -0500
Subject: Sample prep of CdS particles in block co-polymer mycells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd like to prepare some TEM films of 30-50 Angstrom CdS particles that are
in a block-co-polymer solution. Has anyone done this? Could we just pour
them onto a carbon film and let it dry to examine the particles? Any other
ideas?


Thanks,


Robin Griffin
UAB


From daemon Fri Apr 20 14:07:01 2001



From: A. K. Christensen :      akc-at-umich.edu
Date: Fri, 20 Apr 2001 14:57:29 -0400
Subject: Re: Thin sections and glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that glass knives dull very rapidly (either damage or plastic
build-up on the edge), and so we have always tried to get the first few
sections at a given place on the knife edge, which tend to be the best. I
wrote a brief procedure for students years ago, based on that approach, and
have typed it below (the handout had a drawing of a glass knife, with area
A = 1/3 of edge on the side where the whorl meets the edge, area B = middle
1/3 of knife edge, area C = 1/3 of edge at side where whorl is farthest
from the edge):

1. Face the block in area C of knife (see diagram). Cut semithin sections
(if desired) in area B of knife. Then move to area A.

2. Bring the block face parallel with the knife, using the shadow method.
Then use the shadow to bring the block face as close to the knife (but
without touching) as you dare.

3. With the ultramicrotome set for ultrathin sections, manually turn the
microtome wheel quite rapidly until you see the first sign of contact
(usually a sliver off one side of the block face), then stop turning.

4. Turn on automatic sectioning at usual slow cutting speed. Cut about
6-12 full-face sections, then stop and pick them up on EM grids.

5. Retract the stage slightly, move laterally to another place in area A,
and repeat steps 2-4. Continue until area A has all been utilized (or
until you have all the sections you need).

Good luck to you and your students.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 5801 Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Fri, Apr 20, 2001 1:46 PM -0400 Dick Briggs
{rbriggs-at-Science.Smith.edu} wrote:

} I teach an undergraduate-level course in electron microscopy, and
} every year I find that the biggest hurdle for my students, not
} surprisingly, is the cutting of thin sections with glass knives.
} This is of course the point at which the students begin to get very
} discouraged with their individual research projects. I have tried
} various embedding media based on viscosity/penetration demands
} (Spurr's or ultra-low viscosity for plant tissue, for example), but I
} have been unable to come up with a medium that gives students a
} greater chance at success in cutting sections with glass knives.
} Does anyone have a favorite embedding medium that would allow fairly
} routine sectioning of diverse biological samples with glass knives.
} We are using primarily MT-2 microtomes.
}
} I thank you in advance.
}
} Dick Briggs
} Biology Department
} Smith College





From daemon Fri Apr 20 14:10:53 2001



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 20 Apr 2001 14:07:24 -0500
Subject: RE:tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karli, have you considered using magnetic beads to carry your organelles
} through these steps? The process is less disruptive to fragile organelles
} than centrifugation. Whether the gold will settle during centrifugation
} will depend on the size of the gold, an example is 15 nm. gold settles at
} 25,000 rpm for 60 minutes. For a discussion of this issue, you can
consult
} the early papers describing the conjugation of various proteins to
colloidal
} gold,
} Marge

} Margaret Springett
} IEM Specialist
} Electron Microscopy Core Facility
} Mayo Foundation
} email: springett.margaret-at-mayo.edu


} } ----------
} } From: Karli Fitzelle
} } Sent: Thursday, April 19, 2001 2:27 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: TEM Question



From daemon Fri Apr 20 15:44:02 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 20 Apr 2001 16:37:56 -0400
Subject: RE: Sample prep of CdS particles in block co-polymer mycells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin,
Why don't you try polymerizing the block co-polymer and then microtoming the sample. You could get someone over in the med school to do it for you. There are two places on campus that have the facilities and expertise to do it for you, biology, and pathology. The size of the particles would make it very straightforward to do. Talk to Jim Sheetz.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
} [mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com]
} Sent: Friday, April 20, 2001 2:42 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Sample prep of CdS particles in block co-polymer mycells
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyLists } erver/FAQ.html
}
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I'd like to prepare some TEM films of 30-50 Angstrom CdS
} particles that are
} in a block-co-polymer solution. Has anyone done this? Could
} we just pour
} them onto a carbon film and let it dry to examine the
} particles? Any other
} ideas?
}
}
} Thanks,
}
}
} Robin Griffin
} UAB
}


From daemon Fri Apr 20 16:40:55 2001



From: Hayes, Fred :      FHayes-at-TAC.Textron.com
Date: Fri, 20 Apr 2001 17:36:07 -0400
Subject: adhesives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


looking for an adhesive to bond a TPO to another TPO for cryo

any suggestions?

Fred Hayes
FHayes-at-TAC.Textron.com


From daemon Fri Apr 20 17:05:17 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 20 Apr 2001 17:59:41 -0400
Subject: Epitaxy -Am I misinformed or what?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I worked in an MBE group where we grew epitaxial and heteroepitaxial layers on III-V compounds. I thought that I had a pretty good idea of what epitaxy is.

Recently, I have come across a rash of papers that claim epitaxial relationships across dissimilar materials with dissimilar crystal structures. This is just orientational relationships across the interface. It is not epitaxy! Is it because we as materials scientists/microscopists who know better are not getting to review these papers or has there been a change of the definition of epitaxy. What's going on? (This is a rhetorical question posed to foster a discussion.) What are we going to do about it? (another one.) Am I the only one that is concerned that the definition is becoming unclear?

I know that I have made comments about it when I review papers that claim epitaxy when it is really just an orientational relationship.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Fri Apr 20 17:06:10 2001



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 20 Apr 2001 14:59:48 -0700 (Pacific Daylight Time)
Subject: polymerization oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Microscopists,

I am looking for a small oven in which to polymerize epoxy
embedded samples (50-90 C). I'd like one small enough to
fit into our fume cabinet, but the smallest one I can find
is about 13 x 13 x15 inches. Does anyone know of a source
for a smaller oven?

Thanks in advance,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Fri Apr 20 17:47:11 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 20 Apr 2001 15:39:59 -0700
Subject: Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does LKB still manufacture glass strips? If so, from whom does one obtain such glass? If not, what is the best glass available for sectioning and an approximate price? I vaguely remember "LKB glass" and have been using an unspecified "brand X". Thank you for your replies.

Barbara Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster St.
San Francisco, CA 94115
email: Bplowman-at-sf.uop.edu
ph: 415-929-6692



From daemon Fri Apr 20 18:48:13 2001



From: Tom Pella :      tom_pella-at-tedpella.com
Date: Fri, 20 Apr 2001 16:44:14 -0700
Subject: Re: SEM specimen holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might want to try contacting Stuart Enterprises run by Stuart Wisun.
Specimen holders for SEMs are his specialty. His number is (650)424-9089,
and his email address is stuwsn-at-juno.com.

Tom Pella

Raymond Grassl wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Howdy Y'all,
}
} I am looking for an inexpensive vise-style specimen holder for our
} SEM. It does not need to be fancy, just a flat-jawed simple device
} with a screw to tighten the faces of the jaws. After becoming
} frustrated by all the information available on the web,I'm sure
} someone out there could be of assistance. Please help by responding
} online.
}
} Regards,
}
} Ray Grassl
}
} Grassl.raymond-at-basco.com



From daemon Fri Apr 20 21:37:18 2001



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sat, 21 Apr 2001 12:32:27 +1000
Subject: RE: Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LKB is now part of Leica, but LKB never was a glass manufacturer. They marketed
that glass only. Its been sold under the manufacturer's brand name "Alkar" for
many years now. All EM suppliers sell that glass.
We do sell Alkar glass in Australia and New Zealand, but clearly its one item
that you would buy somewhere on your continent.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, April 21, 2001 8:40 AM, Barbara Plowman
[SMTP:Bplowman-at-sfmail.dental.uop.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does LKB still manufacture glass strips? If so, from whom does one obtain
} such glass? If not, what is the best glass available for sectioning and an
} approximate price? I vaguely remember "LKB glass" and have been using an
} unspecified "brand X". Thank you for your replies.
}
} Barbara Plowman
} Univ. of the Pacific
} School of Dentistry
} 2155 Webster St.
} San Francisco, CA 94115
} email: Bplowman-at-sf.uop.edu
} ph: 415-929-6692
}



From daemon Sat Apr 21 02:00:12 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 21 Apr 2001 02:50:45 -0500
Subject: TEM of CdS colloid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robin Griffin wrote:
=============================================================
I'd like to prepare some TEM films of 30-50 Angstrom CdS particles that are
in a block-co-polymer solution. Has anyone done this? Could we just pour
them onto a carbon film and let it dry to examine the particles? Any other
ideas?
==============================================================
What tends to happen is that the polymer is present in sufficient amount
that it interferes with the imaging. Hence you can get around this problem
by putting your preparation on silicon monoxide/dioxide filmed grids, then
in an oxygen plasma etcher, etch away the polymer, leaving only the CdS
colloid dispersed on the support film. However it might be better to put
your solution on a glass slide, allow it to dry, and then etch off the
organics as above, then Pt/C shadow or carbon coat only and pick up the
replica on a grid and examine.

Disclaimer: SPI Supplies manufactures a plasma etcher and also produces
SiO2 filmed grids.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Apr 21 14:52:01 2001



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Sat, 21 Apr 2001 15:42:12 -0400
Subject: Re: Epitaxy -Am I misinformed or what?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott -

Epitaxial growth is the oriented growth of a crystalline material over
another crystalline material [9]. For example, films of fcc Au{001}
can be grown by the deposition of gold on fcc Ag{001} surfaces; the
silver lattice-parameter (4.08 Ang.) is very close to the gold
lattice-parameter (4.09 Ang.). In some case the meshes may not match,
e.g., Tb(0001) grown on W{110}, but incommensurate growth is still
possible. In other cases, the meshes may match by the expansion,
dilation, and rotation of the meshes, as Pb{111} grown on Si{111}.
The success or failure of epitaxial growth depends highly upon the
chosen material's chemical and physical properties, as well as the
surface structure of the substrate.

[9] Epitaxial Growth, J.W. Matthews Academic Press, 1975.

JQuinn

} From: "Walck, Scott D." {walck-at-ppg.com}
} To: "'Microscopy'" {microscopy-at-sparc5.microscopy.com}
} Subject: Epitaxy -Am I misinformed or what?
} Date: Fri, 20 Apr 2001 17:59:41 -0400
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I worked in an MBE group where we grew epitaxial and heteroepitaxial layers on III-V compounds. I thought that I had a pretty good idea of what epitaxy is.
}
} Recently, I have come across a rash of papers that claim epitaxial relationships across dissimilar materials with dissimilar crystal structures. This is just orientational relationships across the interface. It is not epitaxy! Is it because we as materials scientists/microscopists who know better are not getting to review these papers or has there been a change of the definition of epitaxy. What's going on? (This is a rhetorical question posed to foster a discussion.) What are we going to do about it? (another one.) Am I the only one that is concerned that the definition is becoming unclear?
}
} I know that I have made comments about it when I review papers that claim epitaxy when it is really just an orientational relationship.
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
}
}


From daemon Sun Apr 22 11:42:01 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sun, 22 Apr 2001 10:27:24 -0600
Subject: Re: Digital SEM Imaging?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Since Joe's list for some reason unknown to me does not include my company,
I would also like to invite you to take a look at our web site. We provide
both passive and active systems.

Thanks.

Michael Bode
Soft Imaging System Corp.
www.soft-imaging.com
mb-at-soft-imaging.com


-----Original Message-----
} From: "NPGSlithography-at-aol.com"-at-sparc5.microscopy.com
[mailto:"NPGSlithography-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, April 20, 2001 10:13 AM
To: RCHIOVETTI-at-aol.com; Microscopy-at-sparc5.microscopy.com


In a message dated 4/19/2001 3:01:22 AM Mountain Daylight Time,
RCHIOVETTI-at-aol.com-at-sparc5.microscopy.com writes:

} Does anyone know of a digital imaging system for scanning EM?

A list of companies that provide digital imaging systems for SEMs can be
found at
"www.jcnabity.com/links.htm#Digital Imaging".

The two basic types of systems are passive (where they acquire an image
while
the SEM scans) and active (where they control the beam position during the
image acquisition). The passive types will typically be less expensive,
since they do not need to generate the sweep voltages. The active systems
require that the SEM has XY inputs for beam control.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Sun Apr 22 17:33:40 2001



From: Andrew.Campbell3-at-defence.gov.au
Date: Mon, 23 Apr 2001 08:00:27 +1000
Subject: SEC: UNCLASSIFIED:-Unsubscribe

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Please unsubscribe me.

A.R. Campbell




From daemon Sun Apr 22 18:13:26 2001



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Sun, 22 Apr 2001 16:09:38 -0700 (PDT)
Subject: Re: Thin sections and glass knives

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Hi Dick Briggs:
I was a former student at San Francisco State University (I've graduated in
May 2000), and I took an electron microscopy course at that university. My
instructor was Dr. Greg Antipa, and I'm sure that he can give you tips on
plastics he used, etc. Back to my electron microscopy course ... during part
of that course, I was supposed to section some embedded tissue of your
choice (heart / liver / ? (can't recall off-hand)), and I had considerable
trouble using a microtome well.
As I recall, we had some Sorvalls available for use, but I'm unsure if they
were MT-2s.
I prepared all my tissues (and practice, plastic "pellets") by first
cutting with straight razor blades to make an acceptable-sized pyramid, then
continue by sectioning with a microtome.
Glass knives were used on these embedded tissues -- the plastic used was
Epon -- and I recall that one major problem I had was in making sure that
the microtome sectioned at a consistent speed. In addition, the glass knife
edges would dull pretty quickly after a small number of sections were made,
and so it became imperative to use several glass knives for any particular
embedded material. I eventually was able to section pretty well, but I also
picked up sections from below with a grid (of 200 mesh) that I bent rather
badly during the collection process.
Epon worked OK for sectioning, and while it's true that students can indeed
get discouraged, I also know from experience that students have very
different aptitudes towards handling microtomes, preparing the pyramids,
etc.
I hope that my experience with sectioning may help you understand better
(from a student's perspective) the various trouble areas that could occur
when students are first learning how to section embedded material.
Good luck with the sectioning process.
Nelson Conti





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Sun Apr 22 23:03:27 2001



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Sun, 22 Apr 2001 22:57:09 -0500
Subject: ANL HVEM: Retires from Service Mon: Apr. 23, 2001

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On Monday April 23rd 2001 at ~ 9AM CST , after more than two
decades of operation, the ANL HVEM will begin its last experimental
session before permanently shutting down at the end of the day.

On behalf of the hundreds of users in the world wide microscopy research
community who have used this facility since 1979, let me offer thanks to
the ANL crew who has kept this unique resource alive, functional
and running over this period: Hats off to ...Ed Ryan, Stan Ockers,
Charlie Allen,
Tony McCormick, as well as Alan Philippides, Loren Funk, Loren Thompson,
Pete Baldo, and Tony Taylor.

If you would like to peer "live" into the microscope room
via TelePresence and be a small part of the last day of operation of
this unique instrument for Materials Research just point your
Web browser to

http://tpm.amc.anl.gov/HVEM.html


When the last experiment completes later the afternoon of
April 23rd, ANL will begin the decommissioning process and over the next
few weeks the instrument will be completely dismantled. During this time
we will endeavor to keep the live telepresence links operational to broadcast
the decommissioning of this unique resource to allow interested
students/researchers
the opportunity to observe the process.

Coincidentally the last experiment being conducted on the HVEM will be a high
energy electron irradiation damage study similiar in many respects
to the first official experiment conducted at the facility during its opening
ceremonies over twenty years ago.



Nestor J. Zaluzec
Materials Science Division
Argonne National Laboratory



--
===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4289
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From daemon Mon Apr 23 02:04:14 2001



From: =?iso-8859-1?Q?=22S=F8rensen=2C_Susanne=22?=
Date: Mon, 23 Apr 2001 08:57:39 +0200
Subject: Glycolipid

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Does anyone know how to detect glycolipid using LR-White/Cryo
ultrathin-sections?
I have tried both. But in cryosections the lipid is flowing out over the
section, so when I do my immunogold, there is gold all over.
I think that I lose some lipid bound to the membrane, when I use LR-White.
Maybe because of the dehydrasion.
Maybe there is a speciel way to fix the lipid?

S. Sørensen
Herlev Hospital
Denmark


From daemon Mon Apr 23 02:06:54 2001



From: Dr. Klaus Jandt :      K.Jandt-at-bristol.ac.uk
Date: Mon, 23 Apr 2001 08:03:44 +0100
Subject: Postdoctoral Research Assistant

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The Biomaterials Science Group
Department of Oral and Dental Science
University of Bristol
in collaboration with Glaxo SmithKline

Postdoctoral Research Assistant

in the Biomaterials Science Group
on the project
Interaction Mechanisms of Polymers at Interfaces of Mineralised Tissues


The research area involves the study of the physical and chemical properties
and interaction mechanisms of different polymers at interfaces of
mineralised tissues. You will have recently been awarded a PhD in an
appropriate field and will ideally have experience in scanning probe
microscopy (AFM) of biological materials and other analytical techniques and
an interest in medical research. You will work in Dr. Jandts group and
interact with scientists at Glaxo Smith Kline.
The University of Bristol is one of the leading research universities in the
UK and provides an outstanding scientific training environment to enhance
your qualification. The group is involved in exciting, interdisciplinary
projects and maintains appropriate state of the art instrumentation. There
exist opportunities for additional interactions with clinical scientists and
other centres at the university.
We are looking for a dynamic and exceptionally well-qualified postdoctoral
researcher who can interact effectively in an international and
interdisciplinary team. The appointment will be on a Research Assistant 1A
scale with a salary range of # 16775 to # 20465. This is a full time
appointment and initially for one year. Applicants should include a short
CV, stating research experience and interests, publication list and
addresses of two referees. The review of applications will start 24 May 2001
and will continue until the post has been filled.
Informal inquiries can be directed by email to Dr. K. D. Jandt
(K.Jandt-at-bris.ac.uk), Senior Lecturer in Biomaterials, University of Bristol

Formal applications quoting the reference number 7401 should be directed to

The University of Bristol
Recruitment Office
Bristol, BS8 1TH
United Kingdom


-----------------------------------------------------------------
Dr. rer. nat. Klaus D. Jandt
Senior Lecturer in Dental Materials Science and Biomaterials
University of Bristol, Department of Oral and Dental Science
Lower Maudlin Street, Bristol, BS1 2LY, UK
Phone: +44-117-9284418, Fax: ++44-117-9284780
Internet: K.Jandt-at-bris.ac.uk
WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm
"We make Biomaterials Science work!"



From daemon Mon Apr 23 06:51:21 2001



From: =?iso-8859-1?Q?=22S=F8rensen=2C_Susanne=22?=
Date: Mon, 23 Apr 2001 13:45:06 +0200
Subject: TEM, Glycolipid

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Does anyone know how to detect glycolipid using LR-White/Cryo
} ultrathin-sections?
} I have tried both. But in cryosections the lipid is flowing out over the
} section, so when I do my immunogold, there is gold all over.
} I think that I lose some lipid bound to the membrane, when I use LR-White.
} Maybe because of the dehydrasion.
} Maybe there is a speciel way to fix the lipid?

} S. Sørensen
} Herlev Hospital
} Denmark


From daemon Mon Apr 23 07:53:36 2001



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Mon, 23 Apr 2001 07:50:37 -0500
Subject: Re: ANL HVEM: Retires from Service Mon: Apr. 23, 2001

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Hello Nestor & Fellow Microscopists !

What a sad morning this is. I've used this instrument, way
back around 1980, and I can say it's a crying shame that such
machines have such short lives. I found it to be quite useful
for my area of study (finding the orientation distribution,
more properly the misorientation distribution, in the cellular
substructures of highly cold worked metals) for the simple
reason that the selected area aperture of a high-voltage
microscope can be demagnified sufficiently accurately for the
electron beam to be made to illuminate the sub-micron-sized
crystallites.

I've outlived _two_ high-voltage microscopes. Before the ANL
instruments, I was a heavy user of the Million-Volt TEM at the
US Steel Research Center, and for the same reason. Unfortunately,
I was driven to use Argonne's HVEM because US Steel's hourly
charges were too high to fit in my research budget; what with
lack of support for what should have become a regional resource,
the US Steel instrument was soon decommissioned just like ANL's
is about to be.

Best regards,
George Langford, Sc.D.
amenex-at-amenex.com
http://www.amenex.com/


From daemon Mon Apr 23 08:47:42 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Mon, 23 Apr 2001 14:42:23 +0000 (GMT)
Subject: Re: Epitaxy -Am I misinformed or what?

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Hi Scott,
I think the term 'epitaxy' has indistinct boundaries. Okay, so InGaAs on GaAs is undisputedly epitaxy, as long as the crystals line up. But what about GaAs on Si? Most people would say this is epitaxy, but they're different crystal structures (diamond on sphalerite). Would you say something with rock salt structure can't be epitaxial with a diamond structure substrate? And just to stretch it a bit further, how about a (111) cubic layer on a (0001) hexagonal substrate? If not, what about hexagonal CdS on cubic CdS?
My interpretation is that epitaxy requires both a reasonably fixed orientation relationship and a deposition. So MBE silicon on sapphire can be epitaxial, but a martensitic phase transformation can not. Maybe I should look it up in a science dictionary...


Richard

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I worked in an MBE group where we grew epitaxial and heteroepitaxial layers on III-V compounds. I thought that I had a pretty good idea of what epitaxy is.
}
} Recently, I have come across a rash of papers that claim epitaxial relationships across dissimilar materials with dissimilar crystal structures. This is just orientational relationships across the interface. It is not epitaxy! Is it because we as materials scientists/microscopists who know better are not getting to review these papers or has there been a change of the definition of epitaxy. What's going on? (This is a rhetorical question posed to foster a discussion.) What are we going to do about it? (another one.) Am I the only one that is concerned that the definition is becoming unclear?
}
} I know that I have made comments about it when I review papers that claim epitaxy when it is really just an orientational relationship.
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
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Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Mon Apr 23 09:15:25 2001



From: Kevin Mackenzie :      nhi691-at-abdn.ac.uk
Date: Mon, 23 Apr 2001 15:09:25 +0100 (BST)
Subject: 3D reconstruction from 2D sections

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Dear All

We are planning to do some 3D reconstruction of tick salivary glands
from 2D light microscope sections (1 micron thick).

Does anyone have any suggestions for software (PC) that could do this.

thanks

Kevin


Kevin Mackenzie
Electron Microscope unit
Department Zoology
University of Aberdeen
Tillydrone Avenue
Aberdeen
AB24 2TZ
-----------------
Tel 01224 272847
Fax 01224 272396
email k.s.mackenzie-at-abdn.ac.uk
Web Site http://www.abdn.ac.uk/emunit




From daemon Mon Apr 23 09:44:34 2001



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 23 Apr 2001 10:33:41 -0400 (EDT)
Subject: EM Technician wanted

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In June, I will have a mid-level EM Tech position open. One of my folks
is getting married and moving away. We do all TEM--a mixture of negative
staining and thin sectioning; 75% is clinical (diagnosing viral diseases
with the EM, plus surgical pathology EM) and 25% is research. We also do
some teaching of medical and graduate students and residents. We have 5
tech positions, and the 4 remaining individuals are all delightful folks
with whom to work. Durham, NC, is a pleasant place in which to live and
work--3 hrs from the mountains or the beach, and has more entertainment
than you could want with 3 major universities and their arts programs in
close proximity--not to mention the NCAA champs (Go Duke!).

Duke requirements for the position (EM Technician, Senior) include a
bachelor¹s degree and electron microscopy training (a course or
experience) or an associate¹s degree in EM. I am looking for someone who
is proficient in cutting thin sections and has experience running an
electron microscope. We can teach you to operate the particular scope
brand we have, including digital operation for some applications, and we
can teach negative staining and virus recognition. I am also looking for
someone who enjoys challenging and interesting cases, is dedicated to
accuracy, and is willing occasionally to put in extra effort in return
for appropriate compensation and consideration when you have special
needs. And I am particularly looking for someone who can manage several
jobs at once while having a good time sharing camaraderie with the rest
of us, i.e., is not high strung. I know this special person exists,
since there are 4 lovely folks remaining (3 guys and 1 gal) with these
same qualifications. I will be happy to answer any questions you have by
phone or email.

If you might be interested, please contact me directly as the position is
not officially open yet; I have requested that the paperwork be started.
My address and phone number follow.




Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon Apr 23 10:19:10 2001



From: tbargar-at-unmc.edu
Date: Mon, 23 Apr 2001 10:12:10 -0500
Subject: TEM: embedding of Thermanox coverslips

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Hi,
I need advice on embedding Thermanox coverslips. It's supposed to peel off
leaving the monolayer behind, but I'm not having much luck. I'm using
Aralidite 502 as the embedding medium. Would a another resin work better?
I would appreciate any and all advice. Thanks.

Tom Bargar
EM Lab
UNMC
402-559-7347
tbargar-at-unmc.edu



From daemon Mon Apr 23 10:19:56 2001



From: jshields-at-cb.uga.edu
Date: Mon, 23 Apr 2001 11:15:56 -0400
Subject: EM stain of cell wall

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A thread on plant cell wall staining for LM reminded me of a project
wherein I would have liked to differentially stained parts of the plant
cell wall - but for TEM. I could not readily find a good protocol for a
CHO EM "stain" to do this.
Does anyone have some good protocols and ideas for adding
contrast or staining the plant cell wall?

Thanks in advance!

John Shields
EM Lab
University of GA
Athens, GA

jshields-at-cb.uga.edu


From daemon Mon Apr 23 11:01:27 2001



From: David Barnard :      David.Barnard-at-wadsworth.org
Date: Mon, 23 Apr 2001 11:02:26 -0400
Subject: Re: ANL HVEM: Decommissioned from Service Mon: Apr. 23, 2001

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To the entire ANL HVEM crew:

I would like to express my heart felt sadness that another functioning HVEM
will be decommissioned today. As one who has spent over 20 years working
with almost the same microscope here in Albany, NY it is especially
difficult to watch this days events. I know these microscopes like many
know the inner workings of good vintage cars or the DC-3 and its difficult
for me knowing how successfully the the ANL crew has been throughout the
years with this very servicable machine.

There are 6 and then there 5 HVEMs in the US five years ago. Now there
will be only 4!

I'm sure just like today we and NASA marvel at how quickly the technology
and support for the Saturn 5 technology has disapeared even as our space
program is just getting off low earth orbit, we in the microscopy field
will find in the very near future how impossible it is to go back to the
highvoltage technology that gave us that special edge for thick and dense
specimens.

Dave

David Barnard
HVEM
Wadsworth Center
NYS Dept Health
Albany,NY

(518) 473-5299
barnard-at-wadsworth.org




From daemon Mon Apr 23 13:12:48 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 23 Apr 2001 11:10:12 -0700
Subject: Re: 3D reconstruction from 2D sections

Contents Retrieved from Microscopy Listserver Archives
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Autoquant's Autovizualize-3D will do this nicely. The resulting
3D image may be rotated and tilted and undergo further
processing if desired.

Please contact me off-line if you are interested in this
product or need assistance.

Gary Gaugler, Ph.D.
Optical Reflections
916.791.8191
916.791.8186
7970 Twin Rocks Rd
Granite Bay, CA 95746-8111 USA

Disclaimer: I am an authorized reseller of Autoquant's image processing
software products. I mostly handle the Western US. If you have
problems obtaining information on Autoquant products in the UK,
please contact me for assistance.

See http://www.aqi.com for product technical info.


At 07:09 AM 4/23/2001, you wrote:

} Dear All
}
} We are planning to do some 3D reconstruction of tick salivary glands
} from 2D light microscope sections (1 micron thick).
}
} Does anyone have any suggestions for software (PC) that could do this.
}
} thanks
}
} Kevin
}
}
} Kevin Mackenzie
} Electron Microscope unit
} Department Zoology
} University of Aberdeen
} Tillydrone Avenue
} Aberdeen
} AB24 2TZ
} -----------------
} Tel 01224 272847
} Fax 01224 272396
} email k.s.mackenzie-at-abdn.ac.uk
} Web Site http://www.abdn.ac.uk/emunit



From daemon Mon Apr 23 13:55:35 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 23 Apr 2001 13:51:10 -0500
Subject: Re: EDXA, Need help with detector geometry

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We still run a Kevex Quantum on a JEOL 840A SEM. We replaced the Delta V
portion of our analyzer a few years ago, but still have the old chassis
sitting here if you (or anyone else) ever need parts.

I believe most of these concepts were explained in either the Quantex
manual (pg. G-65 for version 5)or in the Tutorial (pg.8-8) manual.

Working distance on an SEM was the distance from pole piece to sample
surface. Fixed distance was the distance from the pole piece to the
centerline of the detector crystal. Therefore, the combination of (WD-FD)
and HD was used to calculate takeoff angle. Therefore, if you cannot get
the exact numbers for your scope, you can probably measure the height
difference between sample and detector and make up some half-reasonable
numbers that generate the correct difference.

Azimuth angle fixed the detector position on the column. Assuming the
sample holder tilts about the y-axis, azimuth was the angle between x-axis
and the detector port. Thus, an azimuth angle of 0 degrees would mean that
your sample tilts directly toward the detector. Our detector was located 23
degrees back of the x-axis. If you never tilt your sample, then azimuth
would not matter. If you do, it helps to correct solid angle and takeoff
angle for the effects of tilt.

We had a tilted detector on our 840. That made things a little trickier. We
had a scale reading to show the distance from detector to sample. But since
our detector was tilted, we could not use that straightaway for horizontal
distance (HD). We had to look up FD and HD values from a table as a
function of scale reading. It was a fairly simple exercise in geometry, but
it always seemed strange that fixed distance (FD) was not really fixed when
the detector was tilted.

I hope this gets you going. If not, just ask for clarification.

Warren

At 09:30 PM 4/19/2001 -0500, you wrote:
} } Subject: EDXA, Need help with detector geometry
} } } Sirs or Madams,
} } }
} } } I am running a JEOL CX II with a Kevex mod. 3200-0018 detector/ Kevex
} Delta Class Anlyzer.
} } }
} } } I am having difficulty locating the geometric variables unique to this
} } } mating of scope and detector. Kevex was unable to supply the data.
} These
} } } geometric variables are used by the analyzer software (Quantex) in
} modeling and subtracting backgrounds.
} } }
} } } The variables I am unable to supply are Working Distance, Fixed Distance,
} } } and Azimuth. I have seen reference to a Quantex Parameters List. This
} } } document was shipped with the original equipment, but alas, this is a
} } second hand scope and the detector was taken from the company warehouse.
} } }
} } } Does anybody use this combination of TEM and detector or know of someone
} } } with this combination? Does anyone wish to share a document listing
} } Quantex parameters for different scopes with Kevex detectors?
} } }
} } } My sanity is in your hands.
} } } I remain humbly yours,
} } }
} } } Stephen Bennett
} } } EMSL Analytical, Inc.
} } } Miami, FL
} } }
} } } miamilab-at-emsl.com
}
} ----------------------
} Warren E. Straszheim
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking



From daemon Mon Apr 23 14:04:26 2001



From: David P Bazett-Jones :      bazett-at-ucalgary.ca
Date: Mon, 23 Apr 2001 12:58:44 -0600
Subject: Job Posting

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Job Posting for Electron Bioimaging Lab, submitted by David P.
Bazett-Jones

Service Manager, Electron Microscopy Facility

Date Posted: April 17, 2001

Position Status: Full-time, Fixed term

Department: Cell BiologyResearch Institute

Available: August 1, 2001

Description of the Position: You will share responsibility for the
operation and maintenance of transmission and scanning electron
microscopes in a new Bioimaging Facility co-sponsored by teaching
hospitals in the University of Toronto. The microscopes include an ESEM
(FEI/Philips) and a 200 kV TEM (FEI/Philips) equipped with EDX, GIF and
cryostage. You will also be responsible for coordination and management

of electron bioimaging services required by investigators of the
Hospital for Sick Children Research Institute.
Qualifications: As an ideal candidate, you have completed a M.Sc. in
biological sciences, or have completed a B.Sc. with experience in
analytical electron microscopy, ultramicrotomy and other sample
preparation techniques. Strong computer skills are an asset.You possess
excellent verbal communication andorganizational skills. You have the
ability to work well independently and in a team.

Hours : 35 hours/week

Salary: $39,848.95 - $50,277.67

Available to: Internal and External Candidates

Deadline: April 25, 2001

Submit Resume to : Erin O’HareThe Hospital for Sick Children,
555 University Avenue, Toronto, OntarioM5G1X8
Fax (416) 813-5671
E-mail: hr.recruiter-at-sickkids.on.ca

Must Quote File Number CG0102-EO We thank you in advance for your
interest. Only those applicants selected for an interview will be
contacted.





From daemon Mon Apr 23 14:27:50 2001



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Mon, 23 Apr 2001 12:25:26 -0700
Subject: Re: TEM: embedding of Thermanox coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tom,
I use Thermanox coverslips a lot here, so here is my advice:
I always place the coverslip inverted on a drop of resin (if I don't,
the coverslip will be "embedded" in the resin and is really hard to
remove) on an aclar sheet. I have also found that the small weigh
dishes, ours are white, are great for keeping many coverslips separate.
Just put one coverslip per dish. They do tend to migrate on the aclar
sheet, especially when the oven isn't level. Usually they peel off very
easily. In the rare instance that they don't, I use a dissecting scope
and choose the area I am
interested it. I then use a razor blade and cut through the resin side
and remove the area of interest. The small piece will come off nicely
and is the exact size and shape I need to remount it on a "blank" block
for sectioning. This is also great because the rest of the sample (cell

culture) remains whole and labelled for identification later.
I hope this helps,
Jo Dee



--
Jo Dee Fish
Coordinator of Electron Microscopy
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620




From daemon Mon Apr 23 20:26:17 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 24 Apr 2001 13:23:50 GMT+1200
Subject: Stability of nitrates

Contents Retrieved from Microscopy Listserver Archives
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Hi there again

Does anyone know if NaNO3 and KNO3 are or are not reasonably stable
under an electron beam?

I want to use them as overlap standards for Na and K respectively,
but would prefer to avoid explosions in the specimen chamber. They
would be mounted on conductive double-sided tape, and carbon coated,
so there is some oxidisable matter available.

Anybody been there?


cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Apr 23 23:59:47 2001



From: Terry Robertson :      terryr-at-cyllene.uwa.edu.au
Date: Tue, 24 Apr 2001 12:52:14 +0800
Subject: What is the solvent for Monastral blue

Contents Retrieved from Microscopy Listserver Archives
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To those biologist out there in cyber space who may have used monastral blue as a marker for macrophages years ago.
Many years ago I purchased 3% monastral blue (copper phalocyanine) in solution from Sigma which we used for in vivo and in vitro phagocytic experiments. Unfortunately Sigma now only sell the powder form of monastral blue. Is there any one who has an old bottle of Sigma 3% monastral blue solution that can tell me what was the solvent used to prepare this solution so that I can dissolve this dye to obtain a 3% stock solution. I have tried every solution that I have in the laboratory to dissolve the powder without success. I need to know exactly what the solvent was in the old Sigma solution. Hope someone out there can help.

Terry Robertson (PhD)




Dr Terry Robertson (PhD)
Senior Research Fellow
Department of Pathology
University of Western Australia
Nedlands 6009

Phone 618 9346 2935
Fax 618 9346 2891
Mobile phone 040302 5440
email terryr-at-cyllene.uwa.edu.au




From daemon Tue Apr 24 04:31:03 2001



From: Cheryl S. Rehfeld :      csr-at-meyerinst.com
Date: Wednesday, April 11, 2001 12:55 PM
Subject: Ask-A-Microscopist:Help Cleaning Lenses

Contents Retrieved from Microscopy Listserver Archives
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The best way to clean immersion oil from a lens is to use lighter fluid.
First remove as much oil from the lens with lens paper. Be gentle; don't
rub. Then dip a cotton swab in the lighter fluid and lightly wipe it across
the lens. All the oil will be removed and it will evaporate very quickly
without leaving a residue or streaks. Xylene is not recommended as it can
dissolve the adhesives holding the lens in place.

Cheryl Rehfeld
Meyer Instruments, Inc.
Leica Distributor
-----Original Message-----
} From: mckaylodge-at-aol.com {mckaylodge-at-aol.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Tue Apr 24 11:08:09 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 24 Apr 2001 10:58:09 -0500
Subject: Re: polymerization oven

Contents Retrieved from Microscopy Listserver Archives
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Doug,

Consider this option to an actual oven: I've used a TEMP-BLOK MODULE HEATER
(by Lab-Line, distributed by Scientific Products) for curing resins. Its a
small heater measuring about 5x8x3 inches, has high and low temperature
ranges with variable control for each. They have a variety of removable
blocks with arrays of wells in them into which you can put BEEM capsules,
Eppendorf tubes, gelatin capsules,etc. I stick a thermometer in one of the
wells to calibrate the tepmerature settings. Place a tight covering of
aluminum foil over the top of the block to better stabilize the temperature
inside.

I have bought them used from our University's scientific apparatus shop
which trades in used lab gear.

Good luck,

Gib

}
} Dear Microscopists,
}
} I am looking for a small oven in which to polymerize epoxy
} embedded samples (50-90 C). I'd like one small enough to
} fit into our fume cabinet, but the smallest one I can find
} is about 13 x 13 x15 inches. Does anyone know of a source
} for a smaller oven?
}
} Thanks in advance,
}
} Doug
} ----------------------
} Douglas R. Keene
} Associate Investigator
} Shriners Hospital Research Facilities
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97201
} phone: 503-221-3434
} FAX: 503-412-6894 (9-5 PST)
} e-mail: DRK-at-shcc.org
}
}
}
}

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Tue Apr 24 12:54:48 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 24 Apr 2001 12:47:34 -0500
Subject: Re: Polymerization oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Doug,

Consider this option to an actual oven: I've used a TEMP-BLOK MODULE HEATER
(by Lab-Line, distributed by Scientific Products) for curing resins. Its a
small electrical heating device measuring about 5x8x3 inches, has high and
low temperature ranges with variable control for each. They have a variety
of removable blocks with arrays of wells in them into which you can put BEEM
capsules, Eppendorf tubes, gelatin capsules,etc. I stick a thermometer in
one of the wells to calibrate the tepmerature settings. Place a tight
covering of aluminum foil over the top of the block to better stabilize the
temperature inside.

I have bought them used from our University's scientific apparatus shop
which trades in used lab gear.

Good luck,

Gib

}
} Dear Microscopists,
}
} I am looking for a small oven in which to polymerize epoxy
} embedded samples (50-90 C). I'd like one small enough to
} fit into our fume cabinet, but the smallest one I can find
} is about 13 x 13 x15 inches. Does anyone know of a source
} for a smaller oven?
}
} Thanks in advance,
}
} Doug} } ----------------------
} Douglas R. Keene
} Associate Investigator
} Shriners Hospital Research Facilities
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97201
} phone: 503-221-3434
} FAX: 503-412-6894 (9-5 PST)
} e-mail: DRK-at-shcc.org
--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-1799 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Tue Apr 24 14:12:24 2001



From: carlabocchese :      carlabocchese-at-bol.com.br
Date: Tue, 24 Apr 2001 15:35:00 -0300
Subject: optical microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to receive informations about seed tissues
separation techniques to analyse under optical
microscopy.
Thanks for your attention
Sinceraly
Carla Bocchese


__________________________________________________________________________
Acesso fácil, rápido e ilimitado? Suporte 24hs? R$19,90?
Só no AcessoBOL - http://www.bol.com.br/acessobol/




From daemon Tue Apr 24 14:26:52 2001



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 24 Apr 2001 12:22:00 -0700
Subject: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


test



From daemon Tue Apr 24 15:02:52 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Tue, 24 Apr 2001 14:48:21 -0500
Subject: recruitment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






One day while walking down the street a highly successful HR Director was
tragically hit by a bus and she died. Her soul arrived up in heaven where
she was met at the Pearly Gates by St. Peter himself.

"Welcome to Heaven," said St. Peter. "Before you get settled in though, it
seems we have a problem. You see, strangely enough, we've never once had a
Human Resources Director make it this far and we're not really sure what to
do with you."

"No problem, just let me in," said the woman.

"Well, I'd like to," replied St. Peter, "but I have higher orders. What
we're going to do is let you have a day in Hell and a day in Heaven and then
you can choose whichever one you want to spend an eternity in."

"Actually, I think I've made up my mind, I prefer to stay in Heaven", said
the woman.

"Sorry, we have rules..." And with that St. Peter put the executive in an
elevator and it went down-down-down to hell. The doors opened and she found
herself stepping out onto the putting green of a beautiful golf course. In
the distance was a country club and standing in front of her were all her
friends - fellow executives that she had worked with and they were all
dressed in evening gowns and cheering for her. They ran up and kissed her on
both cheeks and they talked about old times.

They played an excellent round of golf and at night went to the country club
where she enjoyed an excellent steak and lobster dinner. She met the Devil
who was actually a really nice guy (kinda cute) and she had a great time
telling jokes and dancing. She was having such a good time that before she
knew it, it was time to leave. Everybody shook her hand and waved goodbye as
she got on the elevator. The elevator went up-up-up and opened back up at the
Pearly Gates and she found St. Peter waiting for her.

"Now it's time to spend a day in heaven," he said. So she spent the next 24
hours lounging around on clouds and playing the harp and singing. She had a
great time and before she knew it her 24 hours were up and St Peter came and
got her. "So, you've spent a day in hell and you've spent a day in heaven.
Now you must choose your eternity," he said. The
woman paused for a second and then replied, "Well, I never thought I'd say
this, I mean, Heaven has been really great and all, but I think I had a
better time in Hell." So St. Peter escorted her to the elevator and again
she went down-down-down back to Hell.

When the doors of the elevator opened she found herself standing in a
desolate wasteland covered in garbage and filth. She saw her friends were
dressed in rags and were picking up the garbage and putting it in sacks. The
Devil came up to her and put his arm around her.

"I don't understand," stammered the woman, "yesterday I was here and there
was a golf course and a country club and we ate lobster and we danced and
had a great time. Now all there is is a wasteland of garbage and all my
friends look miserable."

The Devil looked at her and smiled. "Yesterday we were recruiting you, today
you're staff..."












From daemon Tue Apr 24 16:57:18 2001



From: Douglas Keene :      DRK-at-shcc.org
Date: Tue, 24 Apr 2001 14:47:12 -0700 (Pacific Daylight Time)
Subject: Re: TEM: embedding of Thermanox coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You may want to try dipping just the thermanox portion of
your block into liquid nitrogen. The sudden change of
temperature will likely loosen the thermanox away from your
sample. I do not expect epon to be a problem, but I do
know that it works well with Spurrs.

Good luck,

Doug

On Mon, 23 Apr 2001 10:12:10 -0500
"tbargar-at-unmc.edu"-at-sparc5.microscopy.com wrote:

}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} I need advice on embedding Thermanox coverslips. It's
} supposed to peel off leaving the monolayer behind, but I'm
} not having much luck. I'm using Aralidite 502 as the
} embedding medium. Would a another resin work better? I
} would appreciate any and all advice. Thanks.
}
} Tom Bargar
} EM Lab
} UNMC
} 402-559-7347
} tbargar-at-unmc.edu
}
}

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Tue Apr 24 18:17:25 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 24 Apr 2001 16:10:21 -0700
Subject: Search Coil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to find a commercial source for a calibrated search coil to check
stray fields in microscope rooms. Does anyone have any experience with
purchasing this item?

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Tue Apr 24 18:44:39 2001



From: zaluzec-at-aaem.amc.anl.gov
Date: Tue, 24 Apr 2001 18:40:54 -0500
Subject: Fwd: Search Coil/ Magnetic /Acoustic Measurements.

Contents Retrieved from Microscopy Listserver Archives
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John

You can buy calibrated digital magnetic field meters
from F.W. Bell. The are good down to ~ 0.1 mGauss
traceable to NIST. I have the Model 4080 which is a triaxial
field measurement device.

http://www.fwbell.com

For Acoustic Measurements I use the
EXTECH 407727 Digital Sound meter

http://www.extech.com



Nestor

Your Friendly Neighborhood SysOp

===============
}
} I need to find a commercial source for a calibrated search coil to check
} stray fields in microscope rooms. Does anyone have any experience with
} purchasing this item?
}
} John C. Wheatley
} Lab Manager
} Arizona State University
--
===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4289
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From daemon Tue Apr 24 18:55:42 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 24 Apr 2001 16:49:59 -0700
Subject: Search Coil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to find a commercial source for a calibrated search coil to check
stray fields in microscope rooms. Does anyone have any experience with
purchasing this item?

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Wed Apr 25 00:58:32 2001



From: Anaspec :      anaspec-at-icon.co.za
Date: Wed, 25 Apr 2001 07:46:27 +0200
Subject: Search Coil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John
The best bet is to contact your local RS electronics supplier ( find them on
http://www.rs-components.com/ )and ask for the ELF Magnetic Field Strength
meter part number 212 837.
We use it extensively for site tests and have found it to be just as
accurate as using a professional test kit. The only difference between this
unit and a search coil to a scope is that this device simply tells you if
you have a field problem between 20 to 1200Hz where a more expensive search
coil will tell you exactly what frequency it is that is causing the field.

Good Luck
Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com


-----Original Message-----
} From: John C. Wheatley [mailto:John.Wheatley-at-asu.edu]
Sent: 25 April 2001 01:10
To: Microscopy-at-sparc5.microscopy.com


I need to find a commercial source for a calibrated search coil to check
stray fields in microscope rooms. Does anyone have any experience with
purchasing this item?

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu





From daemon Wed Apr 25 03:03:09 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 24 Apr 2001 21:58:02 -1000 (HST)
Subject: Scanners - summary - LONG

Contents Retrieved from Microscopy Listserver Archives
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Here, finally, are the responses I got to the query below:

A colleague has asked for recommendations for setting up a digital
darkroom (fun to spend someone else's money!). This person would benefit
from a really good scanner that could deal with prints, large format
negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
into an Agfa Duoscan T2500. Do any of you have an opinion about this or
other suitable scanners?
##########
**********
We have the Duoscan 1200 but the 2500 is also a very nice unit--additional
(real) resolution and a high O.D. range plus 14 or 16 bit image
depth. The Agfa units also come with a built-in transparency plate
(rather than having to add on a separate transparency adapter). I find
that color fidelity is very good with the Duoscan, and Agfa provides both
reflective and transparency calibration standards. I am currently
scanning in 3x4 TEM negatives at 12 bits (yield is about 26MB per image),
and scan time is fairly rapid. There is one option that you might want to
consider: the DIImage unit is made for up to 4x5 negatives, and I think
(can't remember the last time I read the specs--the neurons aren't firing
today) that resolution is in the 2700 dpi range--even for the 4x5 size.
**********
I have an Agfa duoscan, not the 2500 but a duoscan and I love it. I scan
everything gels, ex-rays, 2x2,line art and em negatives. The scanner is
very versatile. I would recommend it.
**********
Got one, love it, wouldn't trade it for the world. OF course, we haven't
had it long enough for little nitpicky things to start bugging me, but
wow, does it do a nice job. We've scanned Polaroid Type 55 negatives, TEM
negatives, TEM prints, Type 52 prints, and AFM 3-D presentations with
excellent results. We have even scanned in old 35 mm slides (BW and
color) and made prints from them, without enhancement, that are just as
pleasing as the original slides. We don't regret the purchase. We were
able to work through a local photography supply house and obtain a
reconditioned one for a reasonable cost. Our biggest problem right now is
finding a printer that will do it justice without mortgaging the farm.
**********
} From a vendor: We have sold a large number of the Agfa T2500 into this
market with great success.

The T2500 is an excellent scanner for TEM negs. The 2500x2500 optical
resolution is high enough to capture fine details and still allow cropping
or magnification of small areas of the negative. A 3.5 Dmax will capture
details in the dense areas of negatives, slides or prints.

The Duoscan uses what Agfa calls "TwinPlate" design. Unlike most flatbed
scanners which scan both reflective and film originals through the glass
bed, the Duoscan holds films in a drawer, similar to a glassless negative
carrier in an enlarger. No glass means no dust or scratches and no Newton
Rings.

The Agfa Fotolook software driver is also excellent. Setting up scan
parameters is easy and developing custom film terms is
straightforward. You have control over almost every aspect of scanning,
including a wide range of Gamma control which is beneficial for scanning
very low or high contrast TEM negs.

For scanning 35mm slides, a batch holder accommodates up to 20 mounted
35mm slides. Holders are included for 35mm strips, mounted slides,
120/220 films, 4x5 films and a glass plate for odd size or other
transparent originals. I like to scan TEM negs using the 4x5 holder, they
fit across the opening and it works very well.
*******************
I just bought an Epson Perfection 1240U for home use. I am impressed, So
was a professional photographer friend - he was the lab photographer until
made redundant recently.

It has a removable transmitted light box/lid combo, just lift off the
normal lid from its extending hinge slots (to accommodate thickish
documents/thin books?) and fit into the space on the flatbed. It has a
separate light switch which normally cuts off when you finish a job - it
seems.

It comes with a set of (thin, therefore, flimsy) plastic holders for 4 x
5, 120 roll film plus 35 mm and APS (a double strip holder). 35 mm slides
have to be put directly on the flatbed.

It came with free Photoshop LE. "LE" meant no "channels" dialogue box!
********************
We have digital imaging equipment set up to produce photographic quality
prints from transmission electron microscope negatives 6.5cm x 9.0cm,
reproduce photograph prints for posters, prints from slides and of course
convert all types of images suitable for email, to name but a few.
The flat bed scanner is:- AGFA ARCUS II
Slide scanner:- NIKON Coolscan II

Both have given good service. The quality produced by the ARCUS has not
been fully exploited as we feel a compromise between file size and quality
has to be a consideration. The software we use is Adobe Photoshop.
Our equipment is certainly out of date now, but will be interested to hear
what the new machines perform like.
**********
There is a simple rule of thumb I use.

Nominal grain size of film is about 10 microns (varies
with film speed etc but this is the right order of magnitude).
Thus to digitize the film to it's nominal limits your scanner should
be able to digitize to better than this spatial dimension.

A simple back of the envelope calculation says a spatial resolution
of 10 microns is 2540 - dpi..... and as
we all know that must be the optical resolution of the
scanner not the interpolated resolution. Scanners at this
end are obviously more than you need to digitize photo's and
get expensive quickly. Also when you see 2 numbers listed
as the scanners resolution, believe only the first number, that is
the CCD resolution.

Now add your bit depth. 12 bits is the minimum I
would shoot for grayscale image, but if your attempting
diffraction work the higher the better (i.e. 14 -16 bits+).
For color work obviously multiple the bit depth by 3
one for each primary color (RGB). I've seen a number
of 36 bit color scanners but not too many 48 bit ones at
} 2540 dpi.

Lastly, bit depth is irrelevant if you don't have a high
optical density capabilities otherwise your just digitizing
noise. The highest value I believe is an OD of 4.0 but
this is for DRUM scanners. Flatbed scanners typically
run as low as 2.8, upwards to about 3.4 for the best
I've seen in a flatbed.
**********
We have a Duoscan T2500, and I really like the resolution we can achieve
when scanning any transparency media. There is no holder specifically
designed for EM negs, but they fit sideways into the 4x5" holders. It's
great for scanning Kodachromes; I was given a slide with a photo of
someone (very small image) and was able to scan it in, cropped, at 4000
dpi, and turn that file into a 5x7" print without pixellation. Not bad.

The only drawback is that it can be painfully slow when calibrating.
Still, I recommend it as a good, medium-to-high-end film scanner. It's
also an excellent flatbed scanner, but with the low-end units available
today, it's overkill.
**********
Have your colleague check out the Imacon Flextight Precision II
scanner. The optical resolution is 5760 dpi for slide-sized objects;
I believe it drops to 4800 dpi for objects the size of her larger
negatives. The scanner collects 14 bits of usable data per channel,
which can be exported as a two bytes per channel, and has a dynamic
range of 3.9 OD units (4.1 OD max). The machine is also very fast.
The URL is:
http://www.imacon.dk/usr/imacon/wppImacon.nsf/pages/flexprecision.html
**********
If you are scanning EM negatives, you need to keep the dynamic range in
mind. Regular flat beds are closer to 3.2 to 3.4 usually.

The ArtixScan 1100 has a Dmax of 3.9 (about $1600). This was has a 1000 x
2000 dpi resolution. more details at www.microtek.com

The Agfa DuoScan HiD (about $2400) has a 3.7 dynamic range. more details
at www.agfa.com

Nikon has the new CoolScan 8000 that has a 4 or 4.2 dynamic range but it
doesn't hold the large EM negative size - I think it is limited to
something like 2.5 x 3.5 but their website
http://www.klt.co.jp/Nikon/Press_Release/ls-8000_main.html has the
details.

I think I am going to go with the ArtixScan and buy an extra template and
have it machined to hold my size of negatives. Somewhere I saw scary data
showing that it is important to support all 4 edges of the negative or you
get significantly less optimal scans. The ArtixScan comes with 4 holders
but none match my negative size exactly. It has a glass plate holder but
the problem with these and any conventional flat bed scanner is that you
get Newton rings on many or all of the scans if you look closely.

If you are willing to spend $14,000, there is a really neat film scanner
called the Imacon Flextight Precision II CCD Drum Scanner that goes
up to 5600 dpi (true optical) and 4.1 Dmax. I wish I could afford it.
One web site with info about it is
http://www.medgraphix.com/imaconscan.htm

a web site with really strong views on scanning negatives is
http://www.flatbed-scanner-review.org/
**********
I have the HP Photosmart film scanner. It has a scanner of 2400 dpi, for
35 mm film. I think the recommendation of a film scanner is a good one
for the following reason. Some scanner manufacturers make transparency
(slide/negative) devices that use mirrors, but the image quality is poor.
The Dimage or other large format scanners should provide acceptable
images. The catch for large images and high resolution you need a lot of
RAM memory.
**********
I have the Duoscan and a Nikon slide scanner. The Duoscan can scan slides
on the special tray feature but side by side comparisons of the Duoscan
and Nikon show that the Nikon scan is much better. For the larger negs we
had a special tray made for the Duoscan and we scan in our EM negs. The
Nikon has gotten much cheaper and an excellent scanner can be had for $700
with Digital ICE, something you want. Get two scanners.
**********
I love my Epson 1640 scanner, 1600x3200 and up to 4x5 negs and
transparencies.
**********
I was forwarded your inquiry into digital darkrooms by a colleague. I
tackled this issue a few years back and the solution I arrived at is
working out fine. I have been a professional photographer for 12 years. I
work as an imaging specialist/photographer at a Materials Technology
Laboratory.
When our lab went digital (not yet 100%), I purchased what was then a
very good flatbed scanner - Agfa Arcus II. It was a compromise of
sorts. It could handle reflective and transparent originals. It has a max
density of 3.2 and a max optical resolution of 1200 dpi. It is fine for in
house publications and reports but falls short for anything going to a
service bureau. I also don't recommend it for 35mm film. It can scan 35mm
but not to the quality I required. We still use the Agfa for many scanning
tasks but I have since purchased a more capable machine.

The new scanner is a Flextight Precision II, made by Imacon. It has a Dmax
of 4.1, a true optical resolution of 5760 dpi and scans at 14 bits per
colour. I purchased it primarily for it's density range. We have a large
characterization section with a variety of beam instruments but the TEM
negs were always tough to print. Some diffraction patterns take hours to
print in a wet darkroom. I used the TEM negs as test samples for the
scanners I was considering. A weak point of almost all the prospective
film scanners was no holders for TEM film. Imacon has the capability of
accepting custom made holders (Imacon will make them based on client
specs). As well, the Precision II is primarily a film scanner. It will
scan reflective originals up to A4, but I rarely use it for that.

If your colleague is looking for a flat bed scanner, Imacon makes a model
called the Progression. It is equally as capable as the Precision but
appears to handle reflective originals easier( it accepts film originals
from 35mm to 5"x7").It also has a Dmax of 4.1, a true optical resolution
of 5760 dpi and scans at 14 bits per colour. These are both quite a step
up from the T2500. The 2500 boasts a resolution of 5000 dpi but that's
interpolated resolution. I make it a practice not to interpolate when
scanning scientific images because of the addition of false image
information. The 2500 has a Dmax of 3.4 which is quite acceptable for
correctly exposed film or originals with slight underexposure. I don't
think it could handle a "Hail Mary" type of neg. With the Precision II,
I've pulled quality information off a TEM neg in regions where it seemed
transparent to the naked eye. I am very impressed with this machine. I
don't want to seem indifferent to the T2500 however. I believe it is a
good scanner and can handle most jobs with ease. I would also consider the
acquisition software. Fotolook is quite good. I like it's tone curve
editor. But Colorflex packaged with the Imacon scanners allows more manual
control. It has Photoshop-like unsharp mask controls, good colour
correction in all channels, ICC profiles, dot gain compensation etc.

I don't know your colleague's requirements. If he/she is looking for a
capable, affordable desktop flatbed, I think you were quite correct to
recommend the T2500. If he/she is hoping for more capability I would
suggest they look into the Imacon line (www.imacon-usa.com).

The Imacon scanners are comparatively affordable. The Precision II is ~
$14,995 US and the Progression is ~ $19,995 US. I say comparatively
because many comparable scanners are much more expensive ( priced between
$14,000-$150,000). I realize it is a big jump from the $4500 from the T2500. I
justified the expense with not only the quality increase but the time
saved in the darkroom with trouble negatives.
**********
I used Agfa DuoScan HiD earlier and I try to get it here as well. I like
that machine a lot. It's optical resolution is 1000x2000 Dynamic range is
3.7D, which would help scanning DP's. If you want more info you can have a
look at:
http://www.agfa.com/scanners/duoscan_HiD.html
Printing is another task you can buy things from AGFA as well. Their
photoprinter is just excellent, but a bit expensive. I have tried nice HP
inkjet printers with great success.
**********
In response to Tina's post, I have not seen any mention on the list of the
scanner I purchased a few weeks ago, the Epson Expression 1640XL. It has
1600dpi optical resolution (scans at a hardware resolution of 1600x3200
dpi) 42 bit color (14 bit gray) and Dmax of 3.6. It is large format, and
the transparency adapter comes with a range of negative holders. Has SCSI
or USB interfaces with firewire as an optional extra (I use USB on a Win
2000 system). Of course, you pay for what you get - it isn't cheap.

We are only just beginning to learn how best to use all the resolution and
bit depth we now have, but I and my users love it!

This is not a comparison, of course (I haven't used the other models) but
just to say we are happy with what we have.
**********
We are getting first rate resolution results from our "UMAX Powerlock
1100 Magicscan" scanner coupled to a" FUJIX Pictography
3000" printer. Our base computer is always an Apple system upgraded
periodically.
**********
} In response to Tina's post, I have not seen any
} mention on the list of the scanner I purchased
} a few weeks ago, the Epson Expression 1640XL.
} It has 1600dpi optical resolution (scans at
} a hardware resolution of 1600x3200 dpi) 42 bit
} color (14 bit gray) and Dmax of 3.6.

I would certainly believe the resolution and the color depth for
this scanner is adequate, but if scanning TEM films is an issue, I'd
seriously advise measuring the optical density of your films ... I've
heard these approach OD} 4 ... which would imply you might consider the
dedicated film scanners, e.g., Polaroid 45 Ultra or the new Nikon
LS-8000.
**********
I have the scanner you are looking at & like it a lot. To be quite honest
I do not find that I need to exploit it's full capabilities. If I were in
the market again, looking at newer technology I would be interested in a
faster scanner of similar quality. Yes I want my cake & to eat it too :).
I'll give you this analogy. If I have 10 negatives I will franchise my
time, that is let things scan while I hang out in the office doing other
things. If I have 20 negatives, I'll probably go to the darkroom to make
photos. It is quicker & paper is cheaper. BTW I have an Epson 870 inkjet
that produces nice quality images... cost is down to $180 US, (now the
Epson 880)....no financial interest in these companies.
**********
There was a thread recently on scanners for TEM film. I have looked up
all the models mentioned, on the web and called agents for prices - and
produced a comparative table, given below.
I do not guarantee that the figures are accurate but they are my best
interpretation of the data given.
In the light of experience and Nestor's comments, I would suggest that
2000 dpi is a minimum for TEM negatives. You may be able to get away
with less nine times out of ten, but there will be occasions when you
need more.
I would exclude the Minolta and all the Epsons from consideration
(despite the incredibly low prices of some of the Epsons) because of the
low pixel density.
Among the rest the Nikon has the best pixel density and the best optical
density (another critical parameter for TEM negatives). The price is
very competitive too. The Nikon web site does not give a time for
scanning a negative. On the face of it the Nikon would be a best buy -
get a separate, inexpensive flatbed scanner for the other work.
These comments are all my own opinions based on manufacturers' data.
Since we are considering purchase any comments to the contrary would be
most welcome.
Code Maker Model Type
A Agfa DuoScan T2500 Flatbed
-Transparency
included

B Epson 1640 several versions Flatbed
-Transparency
option
1680 several versions

C 1600 several versions Flatbed
-Transparency
included

D Imacon Flextight Precision II Drum -for film and
large
format

E Minolta Dimage ScanMulti II Film

F Nikon Super Coolscan 8000ED Film

G Polaroid 45 Ultra Film

H Umax Powerlook 3000 Flatbed
-Transparency
included





Code dpi OD Time Price
Opinion
at 6 x 9 cm


A 2500 x2500 3.4 3 min $4,500
Fair
B 1600 x 3200 3.6 $300-$3000
Poor
$800-$1400
Poor

C 1600 x 3200 3.3 $650-$1160
Not suitable

D 2240 x2240** 3.9/4.1 N/A above $10k
Good: low pixel density

E 1128 x 1128 3.6
Not suitable

F 4000 x 4000 4.2 N/A $2,695
V. Good

G 2500 x 2500 3.8 5 min $7,495
Good but pricey

H 3048 x 3048 3.6 3 min $6,499

**********
I too am about to buy and I would make a couple of comments on your
evaluation. First, let me remind everyone that the Dynamic range is
a log scale so small numerical differences are significant.

I also think the Nikon Coolscan 8000 looks great but it only takes a
2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers gone
to a smaller film size or is Nikon using a non-Japanese EM as their
standard? seems odd but I don't see how the Nikon would be very
useful. You say a {2000 line scanner would be useful 9 out of 10
times but want the 2000+ lines for the occasional high res scan. I
would argue that the size of the negative was the more important
variable to be worried about. The Nikon couldn't handle 4x5 LM
negatives or transparencies from autoradiography of
Westerns/Northerns, etc.

My leading candidate is the ArtixScan 1100 has a Dmax of 3.9 (about
$1600 with SCSI card). This was has a 1000 x 2000 dpi resolution.
more details at www.microtek.com. This is my leading candidate. It
was 4 negative carriers and I await word whether one could be
modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will have
my scientific instrumentation shop guys fabricate a holder. It comes
with a glass 8 x 10 glass carrier for odd size negs but I want to
avoid Newton rings and want a glassless carrier.

I would appreciate comments on the following argument (I think I have
this correctly figured out but am not sure since so many out there
seem to want to have a higher resolution scanner). I have a Fuji
Pictrography 3000 printer with a 400 dpi output that is as good as
any other widely available printer in the academic world. If you
figure the maximum published image size is about 8 inches, that would
mean the maximum image size be 3200 dpi wide. A 1000 dpi scan of my
negative would be 4500 x 3500 dpi. I could crop by about 28% or 10%
depending on the orientation of the negative and still be taking full
advantage of the printer resolution. In reality, most EM publication
prints are smaller than 8" wide so one could crop even more and still
not need more than 1000 dpi. A resolution } 1000 dpi would be
useful for subtle morphometric analysis but a 4000 dpi scan of a 3 x
4 negative would be 192 MB. That is pretty big for doing morphometry
on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16 MB
and that is much more manageable. Perhaps the difference is in the
type of EM we are doing. I am working with biological specimens
doing standard thin section type stuff. are you doing some Material
Sci application that demands more?
I would love to take advantage of the Firewire option but my
information is that the 8700 has a Dmax of 3.4 vs the 3.9 for the
1100. That is a significant difference. Do EM negatives of
biological thin sections reach that? I think so. I do a lot of EM
immunocytochemistry and have to look for gold (intensely black)
against a very dark tissue component so I am hoping the higher Dmax
improves my results. I frequently scan negatives on a Umax 1100
(Dmax 3.4??) and can't differentiate the gold from the background
although by eye I can discriminate them when the negative is placed
on a light box. Changing my exposure would give me an unusable
image for the rest of the tissue. Maybe this is an extreme case but
I suspect that lots of "dark organelles" (e.g., lysosomes, nuclei)
have fine structure that get lost in the scanning with a low Dmax
scanner.
**********
Your information is correct and mine is not. The Dmax of the 8700 is 3.4.
**********
A colleague and I each recently bought Microtek scanners to scan TEM
negatives. I have the Artixscan 1100 and he has the Model 8700 which has
similar characteristics (actually higher resolution -1200dpi), 3.9 dmax at
42 bits color (14 grayscale), and the glassless film carrier setup. The
8700 has USB and Firewire interfaces and is cheaper ( {$1000), and the 1000
dpi Model 1100 has a SCSI interface. You might want to check out the
specs of the lower cost model 8700 on the microtekusa website if your
computer can handle USB or Firewire.
Both scanners have performed up to our expectations, which I would
characterize as modest. Microtek does not supply a 3-1/4 x 4 " negative
carrier for standard size TEM film but you can easily make a serviceable
one from stiff paper or light cardboard.

How much scanner resolution should you buy? The answer depends on how you
intend to use it. Most applications do not require capturing the full
resolution of the negative. From a practical viewpoint, the scanner
resolution just determines how many times you can magnify the negative
image to produce the final print size. For example, to get a
publication-size print at 300 dpi, an image scanned at 1200 dpi scan could
be zoomed 4X. A practical alternative to spending more for higher
scanning resolution is to take photos at higher
magnification. One exception is with lattice images from the TEM, which
(depending on the lattice fringe spacing on the negative) might require
higher scan resolutions to avoid getting a moire effect. (Of course, not
everyone agrees. My colleague prefers to always scan at the maximum
resolution).

What does a Dmax of 3.9 mean to you? To me it means a very dark
negative. D is the log of the transmitted to incident intensity ratio. I
wonder if users ever actually verify the manufacturer's specs with a
calibrated density target. A Dmax of 3.9 can be useful for scanning TEM
diffraction patterns that might have high contrast, but TEM micrograph
negatives of metals and ceramics generally don't have that much contrast
and biological thin section photos tend to have rather weak contrast. If
your negatives are simply dark, use shorter photo exposure
times. Scanning with maximum allowed grayscale resolutions
(e.g., 14 bits rather than 8) is highly recommended if you intend to
enhance or adjust images, but that's another story.
I believe that those Agfa scanners are OEM by Microtek. If budget is the
concern, I would recommend buying a Microtek Scanmaker 5 ($1,100) instead,
which I have used for scanning quite a few EM negatives and have
satisfactory results. The Dmax and the dynamic range for Scanmaker 5 is
are about 3.7 and 3.4. Another model your colleague might consider is an
Agfa Duoscan HiD ($ 2,500) which has a higher Dmax of 3.7, but less
optical resolution of 1,000 dpi compared with 2500 dpi on a DuoScan
T2500. What others failed to mentioned is that the DuoScan T2500 only has
a narrow strip on the CCD bay being capable of scanning at 2,500 dpi,
otherwise the true optical resolution is 1,000 dpi. Although a lot of
investigators think that the higher scanning resolution, the better, my
personal bias is leaning toward to purchase a scanner having at optical
resolution at 1,000-1,200 dpi. Umax also carries a few mid- to high-end scanners such as
Powerlook III for routine negative stains. My personal experience for the
UMAX scanner is only limited to the Powerlook II, a mid-range scanner
which gives more grayish scanned images compared to those high end models
I mentioned previously. However, it is a descent scanner if you are
working with color transparencies.

**********
I have not summarized the lengthy thread about film (logarithmic) vs
digital (linear) response!


Aloha,
Tina

*************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************







From daemon Wed Apr 25 03:33:11 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 24 Apr 2001 22:17:49 -1000 (HST)
Subject: Digital Darkroom - Summary - Long

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I am helping a former traditional photographic media user/computerphobe
} set up digital imaging capabilities since her university/museum
} department is closing their darkroom facilities and reassigning personnel
} (sigh). She has what appears to be a decent budget (until I started
} pricing the good stuff!). I am proposing she get a fast (733MHz) G4 Mac
} with maximum (1.5GB) RAM, and she saw and fell in love with the Apple
} 22" cinema display (as did I when I saw it in person). People seem to
} like the Agfa T2500 scanner for prints and negatives. A moderate color
} printer,since she has access to other really good printers in her department. A
} Nikon Coolpix 990 digital camera for on- and off-microscope. Photoshop
} 6.0, for which I'll train her. Corel Draw for vector graphics?
**********
Adobe Illustrator or Freehand for vector graphics. Corel Draw is less
common and has a proprietary file format that has caused me troubles
preparing articles for Microscopy Today. Corel can save in other
formats, but people have to use that option.
Also, Adobe has educational pricing, and special package prices that
bundle full versions of Photoshop and Illustrator.

The 733 Mhz G4 Mac is a great idea since it comes with the superdrive
for making DVDs and CDs, which your friend will probably find very
helpful for archiving. I would also encourage getting a copy of
Retrospect for backups. I am a huge fan of HP printers and prefer
them over Epson. I find the print quality as good or a bit better,
and the software engines are much faster, in my experience with HP
970 cxi and Epson 750 and 850.

I also very much like the Nikon 990, although it has taken me a long
time to figure out the best way to use it (almost too many
options....especially for white balance and light metering, but the
quality is very good). Make sure she gets a memory disk for the 990
that is large enough, 64 MB at least. If the memory card is too small
she won't be able to save TIFF files, only JPG. She will need TIFF if
anyone wants to do any sort of quantitative imaging, and the TIFF
files are all well over 2 MB each. Get at least two cards and a USB
card reader (these are cheap) so she can keep the card reader
attached to the Mac, and just swap out memory cards instead of
connecting the camera directly to the Mac, which is kind of a pain.
Make sure she gets the AC adapter, too. Running the Nikon 990 off
batteries is OK if you need to be mobile but in a lab setting
swapping batteries gets old. Alternately, get a battery charger and
extra NiCad batteries and keep the battery charger going all the time
in a handy outlet. Then you avoid the extra AC power cord, which
reduces cord tangle (a pet peeve of mine....).
**********
If you want to do a darkroom for $10K you can do pretty well, especially
if you don't need to include a killer printer. If you need a decent
printer for proofing, etc, I would either use an inkjet or a low/mid end
laser printer. Does she need color output from the local printer?

For the scanner, if she wanted one "great" scanner, the T2500
would be my choice, but here's a few things to consider. What makes a
scanner great depends on the workflow.She should look at her workflow, in particular what amount of
scanning from 35mm will be done? If there is much 35mm, will there be a
large amount of 35mm slides where some type of auto feeder would be
handy? Would scanning from uncut rolls of 35mm film be needed? The mix is
the first thing to figure out. For occasional 35mm scanning the
T2500 would do the job. As the mix of 35mm increases, the value of a
dedicated 35mm scanner increases. One of the best new 35mm scanners
will be the Nikon Supercoolscan 4000ED. 4000dpi res, great Dmax, plus a
slide feeder, long roll holder and even an adapter for glass microscope
slides are available.

On the TEM side, I think the T2500 is still the nuts, especially
for cropping a smaller areas of a neg and still having the resolution to
blow it up for larger prints.

If the scanner budget didn't allow for the T2500 or for the T2500
along with a 35mm scanner, the Duoscan Hi-D would be my next choice. The
Hi-D has slightly lower res(2000x1000) but a high Dmax of 3.7. Agfa's
software is also very powerful.

The first key is probably to figure how much she's going to spend
on the workstation. After that do the printer and/ or the
scanner(s).

Microtek has a new line called Artixscan. When you look at the specs you
will notice many similarities to the Duoscans. This is because Microtek
shares some of Agfa's hardware. Agfa makes their own apochromatic lenses
and the software is different. I personally like Agfa's software better
but Microtek does offer a slight price advantage. Attached is info on the
Microtek 2500 and 1100 scanners.
*************************
I both run the microscope labs here and am a researcher in materials
engineering.

First I want to say that I strongly support the use of the digital
laboratory. While we still occasionally use film for our highest quality
requirements, in general we are fully digital. The use of digital cameras
has really expanded our undergraduate teaching laboratories and has sped
up our research.

I have found one "dark-side" to a digital imaging laboratory as a lab
manager. As the lab manager, I have found that keeping a digital
laboratory up-to-date is much more expensive than the film
laboratory. When we were only film, we had to repair the film cartridges
for our Polaroid PN film (it takes about five minutes) and have the
microscopes cleaned about once a year at a cost of about $1k.

The digital lab. is much more expensive time and repair wise. Because we
crunch our computers with our image size and storage, it takes more of my
time to keep stuff going. All our computers are networked and in addition
to work, the students tend to junk up the computers with downloads etc
which stops them from working for the image processing work. This
requires continual monitoring on my part (in spite of rules against using
them for these applications!) In addition, keeping computers that will
run the data is expensive. I buy pretty much the best out there, but
somehow upgrades are still inevitable. I also have to supply print
cartridges, etc. Researchers always supplied their own film and dark room
supplies. In addition, I've had to have our cameras repaired numerous
times. The cost was high (at least $500) and they stayed gone for up to a
month. Finally, some of my cameras are about 3 years old. I can see a
degradation in the image quality from when they were purchased. The
cameras are much noisier.
I see a future of regular replacement of my cameras in addition to the
computer upgrades. So while the cost to the researchers is lower (which
helps me as a researcher), the cost to the lab itself is higher (which
hurts me as a lab manager). I'm working on setting up a fee schedule for
this equipment but REALLY hate to have to do it. All of you who do this in
a university know how painful it is!

Regarding the camera purchase-in addition to considering the camera
resolution and cost, I think you should consider the image transfer. I
recommend considering a camera with immediate transfer of the image to the
microscope if you have numerous inexperienced users. Being able to focus
on the screen is extremely helpful. The image transfer time is also
important if you have many images to capture. We do image analysis on
numerous images and some of the cameras have about a 30 second transfer
time for decent resolution. This would be unbearable for the number of
images we collect. I'm not sure how the Nikon Coolpix works but this
should be considered by anyone that is purchasing a digital camera.
**********
I just put together a nearly identical system: G4 533MHz, 1.5GB, 22"
conventional monitor (but loved the cinema), Agfa and Polaroid 4x5
scanners, Nikon 990 and Fuji Pro S1, Photoshop, Adobe Illustrator, HP
5000PS poster printer and Adobe In-Design for laying out posters.
Whew.....awesome.

Glad to see that you went with Macintosh. Very wise. Too many people
fall for the empty promises made by the PC Windoze.
**********
I agree that Corel Draw is less common, *especially for
Macintosh*. If you were setting up a PC studio, I'd say otherwise, but
Illustrator and Freehand have been the standard tools for Mac vector
graphics for about a decade. I think Illustrator may have expanded
portability with Photoshop since they're both made by Adobe.
For the most part, Illustrator, Freehand and CorelDraw provide more or
less the same features, they may just call their tools by different names.
Once you know one, it's not too difficult to pick up the others for basic
illustration.
**********
I think you are on the right track. I think you might look at a dedicated
slide scanner too. We got one years ago and it has seen lots of use. So
many people have big collections of slides that they want to turn into
digital images and the slide scanner fills the gap. It is quick, easy, and
doesn't need much training to use. We also have a flat bed scanner, Arcus
II, that gets lots of use, but I am glad we have the slide scanner too.
Arranging slides and cropping etc, can be a pain on the flat bed. With the
slide scanner, just slip in the slide and scan away. Ours is an old
Polaroid Sprint Scan. Today you can get one for pretty cheap that is even
better than ours.

I agree with the recommendation to go with Illustrator over Corel.
Illustrator plays well with Photoshop and has never screwed us up.
Sometimes we run into problems, Canvas has also been a trouble maker.

After a while we started having 'Disk Full' errors on the machine used
with the scanners. Photoshop wants at least 2 - 3 times the size of the
file on the 'Scratch Disk' to swap files etc. If the disk is getting full,
you get stopped by no room on the 'Scratch Disk', same can happen when
trying to print big files, need room to spool the file for printing. So,
get some kind of removable medium, new Macs might have a CD-RW and that
would be cool. I partitioned our big drive, setting aside 1 GB as a
scratch disk where no files or other junk are allowed.

Slightly off the main topic, we have found a wide format printer gets lots
of use. We have an HP 755CM. 36" wide color inkjet. People from all over
use it to make posters for meetings and displays for classes etc.

I think you have started a very good discussion. It is one all of us are
facing and things are changing so fast, we all need to benefit from the
experience of each other. I don't mind at all that you 'introduced a
subject that gets periodically posted here', its a new ballgame just about
every six months.
**********
} From last February(?)

} A broad question for the light microscopists-
}
} I'm writing up a wish list for our EM lab, and it includes (gasp) light
} microscopes. My question is - how do I go about evaluating and choosing a
} digital camera for light microscopes? It would be for both compound and
} dissecting microscopes, should be color, decent resolution, not
} necessarily low light nor real-time video, but capable of good images for
} image analysis on sections. We are getting a confocal, so fluorescence
} imaging would be done there rather than with the proposed 'scope and
} camera.
}
} What do I need to look for, and what price ranges are we talking about?
**********
Here is what I know from my explorations. We got a Kodak MDS 120
system. It is now obsolete and has been replace by a newer, higher
resolution model. Cost was a couple of K. Half the cost was the adapter to
get the camera on to the microscope.

Unlike many 35mm cameras, the lens of most digital cameras is not
removable. So to get the camera to see what the microscope sees, there
needs to be this special adapter gizmo. It is matched to the threads on
the camera at one end and then to the microscope on the other end. The DC
120 is about 1K x 1K res. and does pretty well for up to 5" x 7"
pictures. Even some 8" x 10" are OK. The key is to remember is a photo
'documentation' system. Good for reminding yourself of what you saw and
good enough for most applications, but not the equal of film.

Using it is similar to film, only different. It works best in brightfield
with plenty of light. It is rated at an equivalent of ASA 160. The overall
appearance of the pictures looks harsher to me. The dynamic range seems to
be more compressed than film. Adjusting the light for best contract is
different than using film.

The biggest negative is the way it transfers the digital file from the
camera to the computer. It goes something like this: Set up the camera
like a film camera, fiddle around with the adapter (it is focusable) until
the eyepieces and the camera are parfocal (not easy). If you are pretty
close, take a preview picture. Wait. Wait. The picture has to be
transferred from the camera to the computer. It is not real time like a
video camera. After you wait, the image appears on the computer screen. If
its OK, usually not, take a final high resolution picture. Wait longer
while the larger file is transferred. Once its there, you are done. Ours
uses a Photoshop plug in for acquisition so it easy going from here.

The trouble is that it is more like film in that you don't know if the
picture is good the instant you expose it. Sometimes the light is not just
right, sometimes the focus is a little off. You can't see these things in
real time like you can with a video camera, but a video camera is really
crummy resolution. If everything is set perfectly, then it is like
exposing film, you take the picture and wait, a few seconds to a minute
rather than a few days to a week to see the final result. I never worried
about how the pictures would come out with film, they always did. But
somehow not being able to see the final result instantly with the digital
camera is very frustrating. Maybe since the digital camera is an add on it
is not as well set up and out film camera for focus etc. I did find out
that I had to get some non-adjustable eyepieces so the focus between the
oculars and the camera would not change between users. We went round and
round chasing focus until that was fixed.

This is another problem if you plan to use the camera on different
microscopes. The adapter will have to be adjusted to the new system each
time it is changed. A pain.

All that said, it has been useful having the camera. I learned a lot about
digital photography, some people have gotten good use from it on a
microscope, it is useful for regular photography, and I now can sound like
an expert when discussing the pros and cons of systems with folks here and
abroad who want to buy a system.
**********
I looked at the MDS 290 also, Kodak was offering an 'upgrade' path from
the 120. I passed because the 290's equiv. ASA is 100 vs 160 for the
120. Also the 290 would require USB for image transfer. It is supposed to
be faster, but still won't be video rate. Also to get USB in the room with
the microscope would require a new computer and since the old computer has
a NuBus frame grabber for our video camera I would have to get a new frame
grabber for the PCI bus new computer. So while the camera upgrade was
pretty attractive, the total cost to upgrade was way more than could be
justified by the amount of use that the digital camera has generated so
far.
I have heard a lot of interest in the Nikon Cool Pix 990 (I think that's
the one). It apparently has the ability to send a lower res. B&W signal to
a video monitor so focusing etc can be done in real time, then switch to
high res color for the final digital shot.
******************************

Thanks to everyone who contributed!

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Wed Apr 25 05:03:12 2001



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Wed, 25 Apr 2001 10:57:04 +0100
Subject: TEM: tools for picking up ultrathin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I wondered how commonly people use a tool (like a loop) to pick up
ultrathin sections.

Does it make life easier or does it only introduce different problems?

I was told that I can buy some "luxury" - but am still undecided.

Your views would be very much appreciated.

Regards

Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk
Fax: 44(0)208 547 7562


From daemon Wed Apr 25 05:55:22 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 25 Apr 2001 07:52:06 -0300
Subject: LM: removing Leica historesin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy - Dept. of Botany
E-mail: rinaldop-at-uol.com.br
UFRGS - Porto Alegre - RS
Brazil



From daemon Wed Apr 25 06:44:26 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 25 Apr 2001 04:39:08 -0700 (PDT)
Subject: Re: Scanners - summary - LONG

Contents Retrieved from Microscopy Listserver Archives
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Tina:
Thanks for the time and effort you have invested in this. A most
enlightening and useful summary.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc
On Tue, 24 Apr 2001 21:58:02 -1000 (HST), Tina Carvalho wrote:

| ------------------------------------------------------------------------
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|
|
|
| Here, finally, are the responses I got to the query below:
|
| A colleague has asked for recommendations for setting up a digital
| darkroom (fun to spend someone else's money!). This person would benefit
| from a really good scanner that could deal with prints, large format
| negatives (4"x5", 3.25"x4") as well as 35 mm slides. At one time I looked
| into an Agfa Duoscan T2500. Do any of you have an opinion about this or
| other suitable scanners?
| ##########
| **********
| We have the Duoscan 1200 but the 2500 is also a very nice
unit--additional
| (real) resolution and a high O.D. range plus 14 or 16 bit image
| depth. The Agfa units also come with a built-in transparency plate
| (rather than having to add on a separate transparency adapter). I find
| that color fidelity is very good with the Duoscan, and Agfa provides both
| reflective and transparency calibration standards. I am currently
| scanning in 3x4 TEM negatives at 12 bits (yield is about 26MB per image),
| and scan time is fairly rapid. There is one option that you might want
to
| consider: the DIImage unit is made for up to 4x5 negatives, and I think
| (can't remember the last time I read the specs--the neurons aren't firing
| today) that resolution is in the 2700 dpi range--even for the 4x5 size.
| **********
| I have an Agfa duoscan, not the 2500 but a duoscan and I love it. I scan
| everything gels, ex-rays, 2x2,line art and em negatives. The scanner is
| very versatile. I would recommend it.
| **********
| Got one, love it, wouldn't trade it for the world. OF course, we haven't
| had it long enough for little nitpicky things to start bugging me, but
| wow, does it do a nice job. We've scanned Polaroid Type 55 negatives,
TEM
| negatives, TEM prints, Type 52 prints, and AFM 3-D presentations with
| excellent results. We have even scanned in old 35 mm slides (BW and
| color) and made prints from them, without enhancement, that are just as
| pleasing as the original slides. We don't regret the purchase. We were
| able to work through a local photography supply house and obtain a
| reconditioned one for a reasonable cost. Our biggest problem right now
is
| finding a printer that will do it justice without mortgaging the farm.
| **********
| } From a vendor: We have sold a large number of the Agfa T2500 into this
| market with great success.
|
| The T2500 is an excellent scanner for TEM negs. The 2500x2500 optical
| resolution is high enough to capture fine details and still allow
cropping
| or magnification of small areas of the negative. A 3.5 Dmax will capture
| details in the dense areas of negatives, slides or prints.
|
| The Duoscan uses what Agfa calls "TwinPlate" design. Unlike most flatbed
| scanners which scan both reflective and film originals through the glass
| bed, the Duoscan holds films in a drawer, similar to a glassless negative
| carrier in an enlarger. No glass means no dust or scratches and no
Newton
| Rings.
|
| The Agfa Fotolook software driver is also excellent. Setting up scan
| parameters is easy and developing custom film terms is
| straightforward. You have control over almost every aspect of scanning,
| including a wide range of Gamma control which is beneficial for scanning
| very low or high contrast TEM negs.
|
| For scanning 35mm slides, a batch holder accommodates up to 20 mounted
| 35mm slides. Holders are included for 35mm strips, mounted slides,
| 120/220 films, 4x5 films and a glass plate for odd size or other
| transparent originals. I like to scan TEM negs using the 4x5 holder,
they
| fit across the opening and it works very well.
| *******************
| I just bought an Epson Perfection 1240U for home use. I am impressed, So
| was a professional photographer friend - he was the lab photographer
until
| made redundant recently.
|
| It has a removable transmitted light box/lid combo, just lift off the
| normal lid from its extending hinge slots (to accommodate thickish
| documents/thin books?) and fit into the space on the flatbed. It has a
| separate light switch which normally cuts off when you finish a job - it
| seems.
|
| It comes with a set of (thin, therefore, flimsy) plastic holders for 4 x
| 5, 120 roll film plus 35 mm and APS (a double strip holder). 35 mm
slides
| have to be put directly on the flatbed.
|
| It came with free Photoshop LE. "LE" meant no "channels" dialogue box!
| ********************
| We have digital imaging equipment set up to produce photographic quality
| prints from transmission electron microscope negatives 6.5cm x 9.0cm,
| reproduce photograph prints for posters, prints from slides and of course
| convert all types of images suitable for email, to name but a few.
| The flat bed scanner is:- AGFA ARCUS II
| Slide scanner:- NIKON Coolscan II
|
| Both have given good service. The quality produced by the ARCUS has not
| been fully exploited as we feel a compromise between file size and
quality
| has to be a consideration. The software we use is Adobe Photoshop.
| Our equipment is certainly out of date now, but will be interested to
hear
| what the new machines perform like.
| **********
| There is a simple rule of thumb I use.
|
| Nominal grain size of film is about 10 microns (varies
| with film speed etc but this is the right order of magnitude).
| Thus to digitize the film to it's nominal limits your scanner should
| be able to digitize to better than this spatial dimension.
|
| A simple back of the envelope calculation says a spatial resolution
| of 10 microns is 2540 - dpi..... and as
| we all know that must be the optical resolution of the
| scanner not the interpolated resolution. Scanners at this
| end are obviously more than you need to digitize photo's and
| get expensive quickly. Also when you see 2 numbers listed
| as the scanners resolution, believe only the first number, that is
| the CCD resolution.
|
| Now add your bit depth. 12 bits is the minimum I
| would shoot for grayscale image, but if your attempting
| diffraction work the higher the better (i.e. 14 -16 bits+).
| For color work obviously multiple the bit depth by 3
| one for each primary color (RGB). I've seen a number
| of 36 bit color scanners but not too many 48 bit ones at
| } 2540 dpi.
|
| Lastly, bit depth is irrelevant if you don't have a high
| optical density capabilities otherwise your just digitizing
| noise. The highest value I believe is an OD of 4.0 but
| this is for DRUM scanners. Flatbed scanners typically
| run as low as 2.8, upwards to about 3.4 for the best
| I've seen in a flatbed.
| **********
| We have a Duoscan T2500, and I really like the resolution we can achieve
| when scanning any transparency media. There is no holder specifically
| designed for EM negs, but they fit sideways into the 4x5" holders. It's
| great for scanning Kodachromes; I was given a slide with a photo of
| someone (very small image) and was able to scan it in, cropped, at 4000
| dpi, and turn that file into a 5x7" print without pixellation. Not bad.
|
| The only drawback is that it can be painfully slow when calibrating.
| Still, I recommend it as a good, medium-to-high-end film scanner. It's
| also an excellent flatbed scanner, but with the low-end units available
| today, it's overkill.
| **********
| Have your colleague check out the Imacon Flextight Precision II
| scanner. The optical resolution is 5760 dpi for slide-sized objects;
| I believe it drops to 4800 dpi for objects the size of her larger
| negatives. The scanner collects 14 bits of usable data per channel,
| which can be exported as a two bytes per channel, and has a dynamic
| range of 3.9 OD units (4.1 OD max). The machine is also very fast.
| The URL is:
| http://www.imacon.dk/usr/imacon/wppImacon.nsf/pages/flexprecision.html
| **********
| If you are scanning EM negatives, you need to keep the dynamic range in
| mind. Regular flat beds are closer to 3.2 to 3.4 usually.
|
| The ArtixScan 1100 has a Dmax of 3.9 (about $1600). This was has a 1000
x
| 2000 dpi resolution. more details at www.microtek.com
|
| The Agfa DuoScan HiD (about $2400) has a 3.7 dynamic range. more details
| at www.agfa.com
|
| Nikon has the new CoolScan 8000 that has a 4 or 4.2 dynamic range but it
| doesn't hold the large EM negative size - I think it is limited to
| something like 2.5 x 3.5 but their website
| http://www.klt.co.jp/Nikon/Press_Release/ls-8000_main.html has the
| details.
|
| I think I am going to go with the ArtixScan and buy an extra template and
| have it machined to hold my size of negatives. Somewhere I saw scary
data
| showing that it is important to support all 4 edges of the negative or
you
| get significantly less optimal scans. The ArtixScan comes with 4 holders
| but none match my negative size exactly. It has a glass plate holder but
| the problem with these and any conventional flat bed scanner is that you
| get Newton rings on many or all of the scans if you look closely.
|
| If you are willing to spend $14,000, there is a really neat film scanner
| called the Imacon Flextight Precision II CCD Drum Scanner that goes
| up to 5600 dpi (true optical) and 4.1 Dmax. I wish I could afford it.
| One web site with info about it is
| http://www.medgraphix.com/imaconscan.htm
|
| a web site with really strong views on scanning negatives is
| http://www.flatbed-scanner-review.org/
| **********
| I have the HP Photosmart film scanner. It has a scanner of 2400 dpi, for
| 35 mm film. I think the recommendation of a film scanner is a good one
| for the following reason. Some scanner manufacturers make transparency
| (slide/negative) devices that use mirrors, but the image quality is poor.
| The Dimage or other large format scanners should provide acceptable
| images. The catch for large images and high resolution you need a lot of
| RAM memory.
| **********
| I have the Duoscan and a Nikon slide scanner. The Duoscan can scan
slides
| on the special tray feature but side by side comparisons of the Duoscan
| and Nikon show that the Nikon scan is much better. For the larger negs
we
| had a special tray made for the Duoscan and we scan in our EM negs. The
| Nikon has gotten much cheaper and an excellent scanner can be had for
$700
| with Digital ICE, something you want. Get two scanners.
| **********
| I love my Epson 1640 scanner, 1600x3200 and up to 4x5 negs and
| transparencies.
| **********
| I was forwarded your inquiry into digital darkrooms by a colleague. I
| tackled this issue a few years back and the solution I arrived at is
| working out fine. I have been a professional photographer for 12 years.
I
| work as an imaging specialist/photographer at a Materials Technology
| Laboratory.
| When our lab went digital (not yet 100%), I purchased what was then a
| very good flatbed scanner - Agfa Arcus II. It was a compromise of
| sorts. It could handle reflective and transparent originals. It has a max
| density of 3.2 and a max optical resolution of 1200 dpi. It is fine for
in
| house publications and reports but falls short for anything going to a
| service bureau. I also don't recommend it for 35mm film. It can scan 35mm
| but not to the quality I required. We still use the Agfa for many
scanning
| tasks but I have since purchased a more capable machine.
|
| The new scanner is a Flextight Precision II, made by Imacon. It has a
Dmax
| of 4.1, a true optical resolution of 5760 dpi and scans at 14 bits per
| colour. I purchased it primarily for it's density range. We have a large
| characterization section with a variety of beam instruments but the TEM
| negs were always tough to print. Some diffraction patterns take hours to
| print in a wet darkroom. I used the TEM negs as test samples for the
| scanners I was considering. A weak point of almost all the prospective
| film scanners was no holders for TEM film. Imacon has the capability of
| accepting custom made holders (Imacon will make them based on client
| specs). As well, the Precision II is primarily a film scanner. It will
| scan reflective originals up to A4, but I rarely use it for that.
|
| If your colleague is looking for a flat bed scanner, Imacon makes a model
| called the Progression. It is equally as capable as the Precision but
| appears to handle reflective originals easier( it accepts film originals
| from 35mm to 5"x7").It also has a Dmax of 4.1, a true optical resolution
| of 5760 dpi and scans at 14 bits per colour. These are both quite a step
| up from the T2500. The 2500 boasts a resolution of 5000 dpi but that's
| interpolated resolution. I make it a practice not to interpolate when
| scanning scientific images because of the addition of false image
| information. The 2500 has a Dmax of 3.4 which is quite acceptable for
| correctly exposed film or originals with slight underexposure. I don't
| think it could handle a "Hail Mary" type of neg. With the Precision II,
| I've pulled quality information off a TEM neg in regions where it seemed
| transparent to the naked eye. I am very impressed with this machine. I
| don't want to seem indifferent to the T2500 however. I believe it is a
| good scanner and can handle most jobs with ease. I would also consider
the
| acquisition software. Fotolook is quite good. I like it's tone curve
| editor. But Colorflex packaged with the Imacon scanners allows more
manual
| control. It has Photoshop-like unsharp mask controls, good colour
| correction in all channels, ICC profiles, dot gain compensation etc.
|
| I don't know your colleague's requirements. If he/she is looking for a
| capable, affordable desktop flatbed, I think you were quite correct to
| recommend the T2500. If he/she is hoping for more capability I would
| suggest they look into the Imacon line (www.imacon-usa.com).
|
| The Imacon scanners are comparatively affordable. The Precision II is ~
| $14,995 US and the Progression is ~ $19,995 US. I say comparatively
| because many comparable scanners are much more expensive ( priced between
| $14,000-$150,000). I realize it is a big jump from the $4500 from the
T2500. I
| justified the expense with not only the quality increase but the time
| saved in the darkroom with trouble negatives.
| **********
| I used Agfa DuoScan HiD earlier and I try to get it here as well. I like
| that machine a lot. It's optical resolution is 1000x2000 Dynamic range is
| 3.7D, which would help scanning DP's. If you want more info you can have
a
| look at:
| http://www.agfa.com/scanners/duoscan_HiD.html
| Printing is another task you can buy things from AGFA as well. Their
| photoprinter is just excellent, but a bit expensive. I have tried nice
HP
| inkjet printers with great success.
| **********
| In response to Tina's post, I have not seen any mention on the list of
the
| scanner I purchased a few weeks ago, the Epson Expression 1640XL. It has
| 1600dpi optical resolution (scans at a hardware resolution of 1600x3200
| dpi) 42 bit color (14 bit gray) and Dmax of 3.6. It is large format, and
| the transparency adapter comes with a range of negative holders. Has
SCSI
| or USB interfaces with firewire as an optional extra (I use USB on a Win
| 2000 system). Of course, you pay for what you get - it isn't cheap.
|
| We are only just beginning to learn how best to use all the resolution
and
| bit depth we now have, but I and my users love it!
|
| This is not a comparison, of course (I haven't used the other models) but
| just to say we are happy with what we have.
| **********
| We are getting first rate resolution results from our "UMAX Powerlock
| 1100 Magicscan" scanner coupled to a" FUJIX Pictography
| 3000" printer. Our base computer is always an Apple system upgraded
| periodically.
| **********
| } In response to Tina's post, I have not seen any
| } mention on the list of the scanner I purchased
| } a few weeks ago, the Epson Expression 1640XL.
| } It has 1600dpi optical resolution (scans at
| } a hardware resolution of 1600x3200 dpi) 42 bit
| } color (14 bit gray) and Dmax of 3.6.
|
| I would certainly believe the resolution and the color depth for
| this scanner is adequate, but if scanning TEM films is an issue, I'd
| seriously advise measuring the optical density of your films ... I've
| heard these approach OD} 4 ... which would imply you might consider the
| dedicated film scanners, e.g., Polaroid 45 Ultra or the new Nikon
| LS-8000.
| **********
| I have the scanner you are looking at & like it a lot. To be quite honest
| I do not find that I need to exploit it's full capabilities. If I were in
| the market again, looking at newer technology I would be interested in a
| faster scanner of similar quality. Yes I want my cake & to eat it too
:).
| I'll give you this analogy. If I have 10 negatives I will franchise my
| time, that is let things scan while I hang out in the office doing other
| things. If I have 20 negatives, I'll probably go to the darkroom to make
| photos. It is quicker & paper is cheaper. BTW I have an Epson 870 inkjet
| that produces nice quality images... cost is down to $180 US, (now the
| Epson 880)....no financial interest in these companies.
| **********
| There was a thread recently on scanners for TEM film. I have looked up
| all the models mentioned, on the web and called agents for prices - and
| produced a comparative table, given below.
| I do not guarantee that the figures are accurate but they are my best
| interpretation of the data given.
| In the light of experience and Nestor's comments, I would suggest that
| 2000 dpi is a minimum for TEM negatives. You may be able to get away
| with less nine times out of ten, but there will be occasions when you
| need more.
| I would exclude the Minolta and all the Epsons from consideration
| (despite the incredibly low prices of some of the Epsons) because of the
| low pixel density.
| Among the rest the Nikon has the best pixel density and the best optical
| density (another critical parameter for TEM negatives). The price is
| very competitive too. The Nikon web site does not give a time for
| scanning a negative. On the face of it the Nikon would be a best buy -
| get a separate, inexpensive flatbed scanner for the other work.
| These comments are all my own opinions based on manufacturers' data.
| Since we are considering purchase any comments to the contrary would be
| most welcome.
| Code Maker Model Type
| A Agfa DuoScan T2500 Flatbed
| -Transparency
| included
|
| B Epson 1640 several versions Flatbed
| -Transparency
| option
| 1680 several versions
|
| C 1600 several versions Flatbed
| -Transparency
| included
|
| D Imacon Flextight Precision II Drum -for film
and
| large
| format
|
| E Minolta Dimage ScanMulti II Film
|
| F Nikon Super Coolscan 8000ED Film
|
| G Polaroid 45 Ultra Film
|
| H Umax Powerlook 3000 Flatbed
| -Transparency
| included
|
|
|
|
|
| Code dpi OD Time Price
| Opinion
| at 6 x 9 cm
|
|
| A 2500 x2500 3.4 3 min $4,500
| Fair
| B 1600 x 3200 3.6
$300-$3000
| Poor
|
$800-$1400
| Poor
|
| C 1600 x 3200 3.3
$650-$1160
| Not suitable
|
| D 2240 x2240** 3.9/4.1 N/A above
$10k
| Good: low pixel density
|
| E 1128 x 1128 3.6
| Not suitable
|
| F 4000 x 4000 4.2 N/A $2,695
| V. Good
|
| G 2500 x 2500 3.8 5 min $7,495
| Good but pricey
|
| H 3048 x 3048 3.6 3 min $6,499
|
| **********
| I too am about to buy and I would make a couple of comments on your
| evaluation. First, let me remind everyone that the Dynamic range is
| a log scale so small numerical differences are significant.
|
| I also think the Nikon Coolscan 8000 looks great but it only takes a
| 2.5 x 3.5 negative which is smaller than my JEOL and Hitachi EM
| negative sizes (~ 3 1/2 by 4 1/2"). Have these EM manufacturers gone
| to a smaller film size or is Nikon using a non-Japanese EM as their
| standard? seems odd but I don't see how the Nikon would be very
| useful. You say a {2000 line scanner would be useful 9 out of 10
| times but want the 2000+ lines for the occasional high res scan. I
| would argue that the size of the negative was the more important
| variable to be worried about. The Nikon couldn't handle 4x5 LM
| negatives or transparencies from autoradiography of
| Westerns/Northerns, etc.
|
| My leading candidate is the ArtixScan 1100 has a Dmax of 3.9 (about
| $1600 with SCSI card). This was has a 1000 x 2000 dpi resolution.
| more details at www.microtek.com. This is my leading candidate. It
| was 4 negative carriers and I await word whether one could be
| modified to carry a 3 1/2 by 4 1/2 negative. At worst, I will have
| my scientific instrumentation shop guys fabricate a holder. It comes
| with a glass 8 x 10 glass carrier for odd size negs but I want to
| avoid Newton rings and want a glassless carrier.
|
| I would appreciate comments on the following argument (I think I have
| this correctly figured out but am not sure since so many out there
| seem to want to have a higher resolution scanner). I have a Fuji
| Pictrography 3000 printer with a 400 dpi output that is as good as
| any other widely available printer in the academic world. If you
| figure the maximum published image size is about 8 inches, that would
| mean the maximum image size be 3200 dpi wide. A 1000 dpi scan of my
| negative would be 4500 x 3500 dpi. I could crop by about 28% or 10%
| depending on the orientation of the negative and still be taking full
| advantage of the printer resolution. In reality, most EM publication
| prints are smaller than 8" wide so one could crop even more and still
| not need more than 1000 dpi. A resolution } 1000 dpi would be
| useful for subtle morphometric analysis but a 4000 dpi scan of a 3 x
| 4 negative would be 192 MB. That is pretty big for doing morphometry
| on! A 1000 dpi scan of a 3.5 x 4.5" negative would be about 16 MB
| and that is much more manageable. Perhaps the difference is in the
| type of EM we are doing. I am working with biological specimens
| doing standard thin section type stuff. are you doing some Material
| Sci application that demands more?
| I would love to take advantage of the Firewire option but my
| information is that the 8700 has a Dmax of 3.4 vs the 3.9 for the
| 1100. That is a significant difference. Do EM negatives of
| biological thin sections reach that? I think so. I do a lot of EM
| immunocytochemistry and have to look for gold (intensely black)
| against a very dark tissue component so I am hoping the higher Dmax
| improves my results. I frequently scan negatives on a Umax 1100
| (Dmax 3.4??) and can't differentiate the gold from the background
| although by eye I can discriminate them when the negative is placed
| on a light box. Changing my exposure would give me an unusable
| image for the rest of the tissue. Maybe this is an extreme case but
| I suspect that lots of "dark organelles" (e.g., lysosomes, nuclei)
| have fine structure that get lost in the scanning with a low Dmax
| scanner.
| **********
| Your information is correct and mine is not. The Dmax of the 8700 is
3.4.
| **********
| A colleague and I each recently bought Microtek scanners to scan TEM
| negatives. I have the Artixscan 1100 and he has the Model 8700 which has
| similar characteristics (actually higher resolution -1200dpi), 3.9 dmax
at
| 42 bits color (14 grayscale), and the glassless film carrier setup. The
| 8700 has USB and Firewire interfaces and is cheaper ( {$1000), and the
1000
| dpi Model 1100 has a SCSI interface. You might want to check out the
| specs of the lower cost model 8700 on the microtekusa website if your
| computer can handle USB or Firewire.
| Both scanners have performed up to our expectations, which I would
| characterize as modest. Microtek does not supply a 3-1/4 x 4 " negative
| carrier for standard size TEM film but you can easily make a serviceable
| one from stiff paper or light cardboard.
|
| How much scanner resolution should you buy? The answer depends on how
you
| intend to use it. Most applications do not require capturing the full
| resolution of the negative. From a practical viewpoint, the scanner
| resolution just determines how many times you can magnify the negative
| image to produce the final print size. For example, to get a
| publication-size print at 300 dpi, an image scanned at 1200 dpi scan
could
| be zoomed 4X. A practical alternative to spending more for higher
| scanning resolution is to take photos at higher
| magnification. One exception is with lattice images from the TEM, which
| (depending on the lattice fringe spacing on the negative) might require
| higher scan resolutions to avoid getting a moire effect. (Of course, not
| everyone agrees. My colleague prefers to always scan at the maximum
| resolution).
|
| What does a Dmax of 3.9 mean to you? To me it means a very dark
| negative. D is the log of the transmitted to incident intensity ratio.
I
| wonder if users ever actually verify the manufacturer's specs with a
| calibrated density target. A Dmax of 3.9 can be useful for scanning TEM
| diffraction patterns that might have high contrast, but TEM micrograph
| negatives of metals and ceramics generally don't have that much contrast
| and biological thin section photos tend to have rather weak contrast. If
| your negatives are simply dark, use shorter photo exposure
| times. Scanning with maximum allowed grayscale resolutions
| (e.g., 14 bits rather than 8) is highly recommended if you intend to
| enhance or adjust images, but that's another story.
| I believe that those Agfa scanners are OEM by Microtek. If budget is the
| concern, I would recommend buying a Microtek Scanmaker 5 ($1,100)
instead,
| which I have used for scanning quite a few EM negatives and have
| satisfactory results. The Dmax and the dynamic range for Scanmaker 5 is
| are about 3.7 and 3.4. Another model your colleague might consider is an
| Agfa Duoscan HiD ($ 2,500) which has a higher Dmax of 3.7, but less
| optical resolution of 1,000 dpi compared with 2500 dpi on a DuoScan
| T2500. What others failed to mentioned is that the DuoScan T2500 only has
| a narrow strip on the CCD bay being capable of scanning at 2,500 dpi,
| otherwise the true optical resolution is 1,000 dpi. Although a lot of
| investigators think that the higher scanning resolution, the better, my
| personal bias is leaning toward to purchase a scanner having at optical
| resolution at 1,000-1,200 dpi. Umax also carries a few mid- to high-end
scanners such as
| Powerlook III for routine negative stains. My personal experience for the
| UMAX scanner is only limited to the Powerlook II, a mid-range scanner
| which gives more grayish scanned images compared to those high end models
| I mentioned previously. However, it is a descent scanner if you are
| working with color transparencies.
|
| **********
| I have not summarized the lengthy thread about film (logarithmic) vs
| digital (linear) response!
|
|
| Aloha,
| Tina
|
| *************************************************
| * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
*
| * Biological Electron Microscope Facility * (808) 956-6251
*
| * University of Hawaii at Manoa *
http://www.pbrc.hawaii.edu/bemf*
|
****************************************************************************
|
|
|
|
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed Apr 25 07:24:19 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 25 Apr 2001 09:20:59 -0300
Subject: LM - Removing Leica Historesin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy - Dept. of Botany
E-mail: rinaldop-at-uol.com.br
UFRGS - Porto Alegre - RS
Brazil




From daemon Wed Apr 25 07:25:40 2001



From: Rinaldo Pires dos Santos :      rinaldo-at-ufrgs.br
Date: Wed, 25 Apr 2001 09:23:11 -0300
Subject: LM - Removing Leica Historesin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy - Dept. of Botany
E-mail: rinaldop-at-uol.com.br
UFRGS - Porto Alegre - RS
Brazil




From daemon Wed Apr 25 07:37:29 2001



From: lynni-at-kapiolani.org
Date: Wed, 25 Apr 2001 07:35:49 -0500
Subject: Ask-A-Microscopist: immunohistochemistry on lung tissue

Contents Retrieved from Microscopy Listserver Archives
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Email: lynni-at-kapiolani.org
Name: Lynn Iwamoto

Organization: Kapiolani Medical Center

Education: Graduate College

Location: Honolulu, HI 96826

Question: I am trying to do immunohistochemistry on lung tissue.
What is the best way to fix this tissue? Is there a good reference
for protocols?
Thank you

---------------------------------------------------------------------------


From daemon Wed Apr 25 07:51:21 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Wed, 25 Apr 2001 07:36:36 -0500
Subject: Apology

Contents Retrieved from Microscopy Listserver Archives
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Listers,

A slip of the finger caused me to send an un-topical joke to all.
While no has yet criticized (though some have expressed appreciation
and/or agreement), I feel that the joke was inappropriate to the
venue. My apologies.

Chuck Butterick



From daemon Wed Apr 25 08:45:30 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Wed, 25 Apr 2001 06:40:52 -0700
Subject: TEM: tools for picking up ultrathin sections

Contents Retrieved from Microscopy Listserver Archives
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Dear Claudia,

Go for it! The "magic" loop is great and makes life sooo much easier. I use it
for collecting both the semi-thin sections (instead of an eye lash) and the
thins. Not only you get better control of the position of sections on a grid, it
also saves you time. Although the loop requires very careful handling - it
withstands only certain number of accidents when you bend it from its original
angle - it's worth every penny. Good luck, Alice.


-----Original Message-----
} From: Claudia Hayward-Costa
To: Microscopy-at-sparc5.microscopy.com
Sent: 4/25/01 2:57 AM


Dear Microscopists,

I wondered how commonly people use a tool (like a loop) to pick up
ultrathin sections.

Does it make life easier or does it only introduce different problems?

I was told that I can buy some "luxury" - but am still undecided.

Your views would be very much appreciated.

Regards

Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk
Fax: 44(0)208 547 7562


From daemon Wed Apr 25 09:05:25 2001



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Wed, 25 Apr 2001 09:58:17 -0400
Subject: fields

Contents Retrieved from Microscopy Listserver Archives
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John -

If you are lucky, then you can find the FWBell4080
still with a distributor. However, it was
discontiuned. However, you can still get the 4090,
which has nice output.

If you also go for the Extech sound meter (as per
Nestor's suggestion), then get the high end one.
Model 407355 has software for graphing the results.
It is well worth the extra $.

JQ



}
} From Microscopy-request-at-sparc5.microscopy.com Wed Apr 25 05:22:50 2001
} From: "Anaspec" {anaspec-at-icon.co.za}
} To: "'John C. Wheatley'" {John.Wheatley-at-asu.edu} ,
} {Microscopy-at-sparc5.microscopy.com}
} Subject: RE: Search Coil
} Date: Wed, 25 Apr 2001 07:46:27 +0200
}
}
} Hi John
} The best bet is to contact your local RS electronics supplier ( find them on
} http://www.rs-components.com/ )and ask for the ELF Magnetic Field Strength
} meter part number 212 837.
} We use it extensively for site tests and have found it to be just as
} accurate as using a professional test kit. The only difference between this
} unit and a search coil to a scope is that this device simply tells you if
} you have a field problem between 20 to 1200Hz where a more expensive search
} coil will tell you exactly what frequency it is that is causing the field.
}
} Good Luck
} Luc Harmsen
} Anaspec, South Africa
} Technical support on microscopy.
} Tel + 27 (0) 11 476 3455
} Fax + 27 (0) 11 476 7290
} anaspec-at-icon.co.za
} www.anaspec.co.za
}
} } -----Original Message-----
} } From: John C. Wheatley [mailto:John.Wheatley-at-asu.edu]
} } Sent: 25 April 2001 01:10
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Search Coil
} }
} }
} } I need to find a commercial source for a calibrated search coil to check
} } stray fields in microscope rooms. Does anyone have any experience with
} } purchasing this item?
} }
} } John C. Wheatley
} } Lab Manager
} } Arizona State University
} } Center for Solid State Science
} } PSA-213
} } BOX 871704
} } Tempe, AZ 85287-1704
} }
} }
} } Phone: (480) 965-3831
} } FAX: (480) 965-9004
} } John.Wheatley-at-ASU.Edu
} }
} }
} }
} }


From daemon Wed Apr 25 09:36:31 2001



From: sghoshro-at-NMSU.Edu
Date: Wed, 25 Apr 2001 08:31:41 -0600 (MDT)
Subject: Re: TEM: tools for picking up ultrathin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Claudia,

Until recently, I was very much against using a loop to pick up ultrathin
sections. A student in the lab started using it and I kind of reluctantly
gave it a try. Now I am a regular user of the loop to pick up sections on
coated grids.

First I clean the loop with 100% ethanol, air dry and pick up sections. I
place a coated grid on a piece of filter paper and bring the loop with the
sections down onto the coated grid and let the filter paper soak the water
droplet. The sandwiched grids are allowed to air dry for few minutes and
you can then remove the loop using a fine tweezer. It works just great.

I bought my loop from Electron Microscopy Sciences. No financial interest
with EMS.

Just give it a try.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml

On Wed, 25 Apr 2001, Claudia Hayward-Costa wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopists,
}
} I wondered how commonly people use a tool (like a loop) to pick up
} ultrathin sections.
}
} Does it make life easier or does it only introduce different problems?
}
} I was told that I can buy some "luxury" - but am still undecided.
}
} Your views would be very much appreciated.
}
} Regards
}
} Claudia
}
} Dr. C. Hayward-Costa
} School of Life Sciences
} Kingston University
} Penrhyn Road, Kingston upon Thames
} Surrey KT1 2EE, UK
} 44(0)208 547 2000 x 2240
} Email: c.hayward-at-kingston.ac.uk
} Fax: 44(0)208 547 7562
}
}



From daemon Wed Apr 25 10:03:38 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 25 Apr 2001 12:00:34 -0300
Subject: Removing Leica Historesin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy - Dept. of Botany
E-mail: rinaldop-at-uol.com.br
UFRGS - Porto Alegre - RS
Brazil



From daemon Wed Apr 25 10:07:02 2001



From: NPGSlithography-at-aol.com
Date: Wed, 25 Apr 2001 11:02:32 EDT
Subject: Re: Search Coil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 4/24/2001 9:41:00 PM Mountain Daylight Time,
John.Wheatley-at-asu.edu writes:

} I need to find a commercial source for a calibrated search coil to check
} stray fields in microscope rooms. Does anyone have any experience with
} purchasing this item?

An inexpensive (~$90) digital gauss meter (Extech Model #480823) that is
quite adequate for locating sources of 30 to 300 Hz magnetic fields can be
found at Meters and Instruments, "www.MetersandInstruments.com", (800)
773-0370.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Wed Apr 25 10:29:28 2001



From: joachim.prutsch-at-leica-microsystems.com
Date: Wed, 25 Apr 2001 17:24:21 +0200
Subject: Antwort: TEM: tools for picking up ultrathin sections

Contents Retrieved from Microscopy Listserver Archives
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Hi Claudia,

I think it really depends on what you prefer ...

Picking up from above is easy with the grid held by a forceps (I prefer a
curved Dumont 5 or 7 with a rubber "clamp" to hold it together), the
sections just "jump" on my filmed grid.
I have tried a Perfect Loop (commercially available) and I also liked it -
I could see no difference in "section quality" comparing both methods.
Picking up with a grid from below the water surface I do not like
(especially with filmed grids, but thats just my personal opinion)

I sometimes loose sections when I am not carefull enough with my eyelash
but very very rarely when picking up...

Of course for cryosectioning a loop is an absolute must - and here I
clearly prefer the Perfect Loop! The droplet of sucrose solution it can
hold is much larger than in a homemade wire loop (gives you a bit more time
in the cryochamber) and you can nicely use this large drop as a
magnification lens for locating your cryoscetions before picking them up.

Best regards,

Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems GmbH
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A 1170 Vienna Tel.: +43 1 4 88 99 - 235
AUSTRIA Fax: +43 1 4 88 99 - 350




From daemon Wed Apr 25 10:32:21 2001



From: jshields-at-cb.uga.edu
Date: Wed, 25 Apr 2001 11:28:38 -0400
Subject: Re: TEM: embedding of Thermanox coverslips

Contents Retrieved from Microscopy Listserver Archives
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This reminds me - we used to get Epon or Spurr's off sandwiched
glass slides by putting them in -20C freezer for 1/2 hr and then
work the sample off.
John Shields
EM Lab
Univ. of GA
Athens

On 24 Apr 2001, at 14:47, Douglas Keene wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
}
} You may want to try dipping just the thermanox portion of
} your block into liquid nitrogen. The sudden change of
} temperature will likely loosen the thermanox away from your
} sample. I do not expect epon to be a problem, but I do
} know that it works well with Spurrs.
}
} Good luck,
}
} Doug
}
} On Mon, 23 Apr 2001 10:12:10 -0500
} "tbargar-at-unmc.edu"-at-sparc5.microscopy.com wrote:
}
} }
} } --------------------------------------------------------------------
} } ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} } ---.
} }
} }
} } Hi,
} } I need advice on embedding Thermanox coverslips. It's
} } supposed to peel off leaving the monolayer behind, but I'm
} } not having much luck. I'm using Aralidite 502 as the
} } embedding medium. Would a another resin work better? I
} } would appreciate any and all advice. Thanks.
} }
} } Tom Bargar
} } EM Lab
} } UNMC
} } 402-559-7347
} } tbargar-at-unmc.edu
} }
} }
}
} ----------------------
} Douglas R. Keene
} Associate Investigator
} Shriners Hospital Research Facilities
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97201
} phone: 503-221-3434
} FAX: 503-412-6894 (9-5 PST)
} e-mail: DRK-at-shcc.org
}
}




From daemon Wed Apr 25 11:00:18 2001



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Wed, 25 Apr 2001 16:55:51 +0100
Subject: epoxy for FEGTEM samples

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
What's the best 'glue' to use for semiconductor cross section sample prep?
We use Gatan G1 to stick the 'sandwich' together (bake at about 150C for an
hour) BUT then we stick a 3mm supporting washer onto the mechanically
thinned sample using Devcon 5 Minute epoxy prior to ion beam milling.
In our constant search for less 'contamination', we are not sure if this is good
practice in a FEG (especially) TEM. Are there any other alternative 'glues'
that would do the job - without baking?
Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365 Mob: 07796 055149
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************


From daemon Wed Apr 25 13:22:48 2001



From: JHoffpa464-at-aol.com
Date: Wed, 25 Apr 2001 14:15:49 EDT
Subject: bx turn around times

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--part1_e0.13c81b16.28186e55_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

A quick question for the clinical diagnostic people out there. I was
wondering what an average turn around time would be for an EM specimen. that
is from time of arrival to the time it is placed in the scope.

--part1_e0.13c81b16.28186e55_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} A quick question for the clinical diagnostic people out there. I was
{BR} wondering what an average turn around time would be for an EM specimen. that
{BR} is from time of arrival to the time it is placed in the scope. {/FONT} {/HTML}

--part1_e0.13c81b16.28186e55_boundary--


From daemon Wed Apr 25 13:31:53 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Wed, 25 Apr 2001 08:40:42 -0400
Subject: Donating a microscope

Contents Retrieved from Microscopy Listserver Archives
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I am trying to find a place to donate my father's microscope. He was a
physician, born in Germany. The microscope was bought in Germany, probably
in the 1920's or 1930's. It is stored in a wooden case. Is there any
place
this instrument could be useful? A school or university in the third
world?
A school in the US?

Please contact me, offline, at ulmithaca-at-home.com.

Thanks.

Gottfried Neuhaus




From daemon Wed Apr 25 14:06:18 2001



From: Pombo, Ana :      ana.pombo-at-csc.mrc.ac.uk
Date: Wed, 25 Apr 2001 20:01:37 +0100
Subject: Jobs: Microscopy and Flow Cytometry Core Positions at MRC Clinica

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The Microscopy and Flow Cytometry Core Facility within the MRC Clinical
Sciences Centre, Imperial College School of Medicine, has immediate openings
for:

Microscopy Research Associate/Assistant (Ref: MRA)
Microscopy Core Facility

The imaging of molecules within cells, organs and whole organisms is at the
forefront of scientific developments in the life sciences. The Clinical
Sciences Centre (CSC) is a leading research institute with groups developing
new avenues in the areas of confocal laser scanning microscopy (CLSM),
live-cell imaging, scanning ion conductance microscopy, position emission
tomography (PET), and nuclear magnetic resonance (NMR).
The MRC is seeking a highly motivated individual to be responsible for this
state-of-the-art microscope core facility at the CSC, to provide full
support for confocal, live-cell imaging, and other fluorescence microscope
users within the institute. The successful candidate should have a science
degree in a relevant subject. Experience in confocal, live-cell and digital
imaging (particularly Leica confocal microscope and/or deconvolution
software) would be highly desirable but not essential, as training can be
given.
Duties will include direct technical support, training and supervision of
users. Involvement in research project using fluorescence microscopy will be
encouraged (further information about research at the CSC can be found at
www.csc.mrc.ac.uk).
Informal enquiries and further information about the position to Drs Ana
Pombo (tel: +0/44 20 8383 8232; ana.pombo-at-csc.mrc.ac.uk) or Alex Sardini
(tel: +0/44 20 8383 8270, a.sardini-at-csc.mrc.ac.uk), MRC Clinical Sciences
Centre, Imperial College School of Medicine, Hammersmith Campus, Du Cane
Road, London W12 0NN. The closing date for applications is 30 April 2001.

Research Assistant (Ref: FCRA)
Flow Cytometry Core Facility

Flow cytometric analysis and cell sorting are important for many areas of
research in the CSC, including immunology, gene expression and stem cell
work. The facility is operated jointly with Imperial College School of
Medicine (ICSM) and consists of 2 FACS Vantage cell sorters and several
bench top analysers. The MRC is seeking a full time research assistant to
help our existing staff provide a cell sorting and analysis service , advise
users on the design of their experiments and liase with service engineers
(all instruments are on full service contracts). Involvement in research
projects will be encouraged. The successful candidate will have a strong
interest in the interface between biology and technology. Experience in flow
cytometry will be an advantage but is not essential as training can be
given.
Informal enquiries and further information about the position to Dr Matthias
Merkenschlager (tel: +0/44 20 8383 8236/9;
matthias.merkenschlager-at-csc.mrc.ac.uk), MRC Clinical Sciences Centre,
Imperial College School of Medicine, Hammersmith Campus, Du Cane Road,
London W12 0NN. The closing date for applications is 30 April 2001.

The CSC is an institute funded by the Medical Research Council and forms
part of the ICMS, based at the Hammersmith Hospital in West London. This
recently established centre has first class facilities and provides
investigators from clinical and basic science backgrounds with the
opportunity to pursue innovative, multidisciplinary research within the
established clinical base of ICMS.
Salaries will be on the MRC's own pay scales and will be commensurate with
experience.
Further details on how to apply are available from the Human Resources Group
tel: 020 8383 3446/7, quoting the relevant reference above.


Advertised in Nature and New Scientist.


Ana Pombo, D.Phil.
Nuclear Organisation Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital Campus
Du Cane Road
London W12 0NN
UK

Tel. (+0/44) 20 83838232 (office)
(+0/44) 20 83838326 (lab.)
Fax. (+0/44) 20 83838337
Web: http://www.csc.mrc.ac.uk/research/Nuclear/Organisation.html



From daemon Wed Apr 25 14:14:13 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Wed, 25 Apr 2001 14:08:47 -0500
Subject: Replacement block holder for Sorvall

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists:

I need to replace a vise style block holder for a Sorvall microtome.

Any contacts out there?

Thanks

T Quinn
Kansas University
Museum of Natural History
tquinn-at-ukans.edu
765-864-4556


From daemon Wed Apr 25 14:29:06 2001



From: Eric Stach :      EAStach-at-lbl.gov
Date: Wed, 25 Apr 2001 12:23:49 -0700
Subject: Re: [ANL HVEM: Decommissioned from Service Mon: Apr. 23, 2001]

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Fellow Microscopists:

Although the 1.2MeV HVEM at ANL has sadly departed, we at NCEM want to
remind everyone on the listserver that there still exists a HVEM in the US
available for on-site and remote use, free of charge with approved proposal.

The Kratos 1.5 MeV HVEM has recently been refurbished (following some
substantial high voltage and vacuum issues). It is presently operational at
1 MeV, and is being conditioned for use at 1.5 MeV. 1.5 MeV operation is
expected by the end of June, at the latest.

NCEM is actively seeking user proposals for this instrument. Please see:

http://ncem.lbl.gov

http://ncem.lbl.gov/frames/hvem.htm

for further details regarding the instrument's many capabilities (among them
straining stage experimentation and environmental cell work), or feel
free to contact either myself or Doug Owen - DKOwne-at-LBL.gov

Regards,
Eric Stach
--
Eric A. Stach
Staff Scientist
National Center for Electron Microscopy
Mail Stop 72-150
Lawrence Berkeley National Laboratory
Berkeley, CA 94720
Phone: 510.486.4634
Fax: 510.486.5888
http://ncem.lbl.gov




From daemon Wed Apr 25 14:55:04 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Wed, 25 Apr 2001 14:17:21 -0400
Subject: Donating a microscope

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I am trying to find a place to donate my father's microscope. He was a
physician, born in Germany. The microscope was bought in Germany, probably
in the 1920's or 1930's. It is stored in a wooden case. Is there any
place
this instrument could be useful? A school or university in the third
world?
A school in the US?

Please contact me, offline, at ulmithaca-at-home.com.

Thanks.

Gottfried Neuhaus




From daemon Wed Apr 25 15:42:10 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 25 Apr 2001 13:36:16 -0700
Subject: Re: epoxy for FEGTEM samples

Contents Retrieved from Microscopy Listserver Archives
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Dear Alan,
In the original semi-conductor cross-section discussions, M-Bond 610 was
recommended. It is a srain-gauge glue, two-part, that requires about 100
deg. C to harden, but it is low viscosity and left a very thin joint. When
we were waitng for our order to arrive, we used Devcon 2-ton epoxy, which is
higher viscosity and requires eight hours to harden, but still made a thin
joint if you squeezed it in a parallel-jawed vice. Once these are hard, we
saw no evidence of out-gassing, but we do not have a FEGTEM.
At 04:55 PM 4/25/01 +0100, you wrote:
}
} Hi Listers,
} What's the best 'glue' to use for semiconductor cross section sample prep?
} We use Gatan G1 to stick the 'sandwich' together (bake at about 150C for an
} hour) BUT then we stick a 3mm supporting washer onto the mechanically
} thinned sample using Devcon 5 Minute epoxy prior to ion beam milling.
} In our constant search for less 'contamination', we are not sure if this is
good
} practice in a FEG (especially) TEM. Are there any other alternative 'glues'
} that would do the job - without baking?
} Alan Walker

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Apr 25 16:26:34 2001



From: PMarcum :      pmarcum-at-p3.net
Date: Wed, 25 Apr 2001 17:18:45 -0400
Subject: LM - Removing Leica Historesin

Contents Retrieved from Microscopy Listserver Archives
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Technically you can not remove this type of plastic from a section. Several
methods have been published and suggested however, they often destroy the
section at the same time.
Pam Marcum

-----Original Message-----
} From: Rinaldo Pires dos Santos [mailto:rinaldo-at-ufrgs.br]
Sent: Wednesday, April 25, 2001 8:23 AM
To: Listserv Microscopy


Hi all,

Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.

Dr. Rinaldo Pires dos Santos
Lab. of Plant Anatomy - Dept. of Botany
E-mail: rinaldop-at-uol.com.br
UFRGS - Porto Alegre - RS
Brazil





From daemon Wed Apr 25 16:51:57 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 25 Apr 2001 17:46:48 -0400
Subject: epoxy for FEGTEM samples

Contents Retrieved from Microscopy Listserver Archives
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If you are not doing site-specific work with semiconductors as I assume that you are from your message, I would suggest that you use the small angle cleavage technique to do your cross sections. I have done III-V and Si based samples with superb results. Take a look at John McCaffrey and my article in the Number IV MRS TEM Sample Prep book, Vol 480, 1997 for a detailed procedure. South Bay Technology sells the "Micro Cleave Kit". This technique is inexpensive and gives great samples. I have used the samples with no plasma cleaning in a FEGTEM without any problems. In addition, you have no ion milling damage. In fact for a FEG, you have a disadvantage because there is little or no amorphous area to use for focusing.

I use the H-22 silver epoxy from Epoxy Technology to put the samples on to the grids. This requires heating. When I do not want to heat the samples, I have used a Duro 90 minute epoxy for long working times. Unfortunately, it takes about 12 hours to fully cure. I have also used these samples in a FEGTEM without problems. I have cured them in atmosphere and in a nitrogen atmosphere.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: A.Walker [mailto:Alan.Walker-at-sheffield.ac.uk]
Sent: Wednesday, April 25, 2001 11:56 AM
To: Microscopy-at-sparc5.microscopy.com


Hi Listers,
What's the best 'glue' to use for semiconductor cross section sample prep?
We use Gatan G1 to stick the 'sandwich' together (bake at about 150C for an
hour) BUT then we stick a 3mm supporting washer onto the mechanically
thinned sample using Devcon 5 Minute epoxy prior to ion beam milling.
In our constant search for less 'contamination', we are not sure if this is good
practice in a FEG (especially) TEM. Are there any other alternative 'glues'
that would do the job - without baking?
Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365 Mob: 07796 055149
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************


From daemon Wed Apr 25 18:40:21 2001



From: Steve Beck :      becks-at-sunynassau.edu
Date: Wed, 25 Apr 2001 22:02:22 -0400
Subject: Summer 2001 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
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The loop works great!!! It's kind of pricy, so careful handling is a must,
but it can be a real headache saver. I, too, bought my loop from Electron
Microscopy Sciences. I have no financial interest in EMS.

Good luck,

Elizabeth P. Bray
Plant Chemist, Central Laboratory
South Carolina Electric and Gas Co.
2102 N. Lake Dr.
Columbia, SC 29212

----- Original Message -----
} From: "Claudia Hayward-Costa" {LS_S562-at-crystal.kingston.ac.uk}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, April 25, 2001 5:57 AM


I rather enjoyed it.

Earl

----- Original Message -----
} From: "Chuck Butterick" {cbutte-at-ameripol.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, April 25, 2001 5:36 AM


SUMMER I 2001 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section B)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I 2001 semester, course in Biological
Transmission Electron Microscopy is being offered by the Biology Department
of Nassau Community College. This is a 4 credit course offered four days
per week (Monday through Thursday) between the hours of 8:00 am and NOON.
Classes will begin on May 29 and end on June 28, 2001.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$92 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/sum01/away.pdf. The phone
registration option is available until tomorrow 4/26/01 (6:30 PM) by
calling 516-572-7131 or 7372 or 7425.

P.S. A Fall 2001 TEM course is also being offered (BIO 221 - Section E2) on
Thursday evenings beginning at 5:30 PM.


Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Wed Apr 25 21:42:09 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 25 Apr 2001 22:38:39 -0500
Subject: Removal of hydroxyethylmetacrylate resins

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Dr. Rinaldo Pires dos Santos wrote:
=================================================================
Is there some procedure for to remove hydroxyethylmetacrylate resins (Leica
Historesin) from semithin sections (1-2 micrometers)?
Thank you.
==================================================================
Perhaps there are other methods, but one that has been used quite
successfully has been the use of plasma etching in a unit such as the SPI
Plasma Prep™ II.

Some examples are on the SPI Supplies website. For example, on URL
http://www.2spi.com/catalog/instruments/etchers1.html
you will see an example of a thick section: "Statocyst organ etched 3 min.
using O2", and of a thin section, "Low temperature oxygen plasma etched
thin section of bacterium embedded in SPI Chem Low Acid GMA for TEM."

Like with any technique in LM or EM, this approach has its limitations. But
it is a good way, at least in some instances to remove GMA without
disturbing the rest of the sample.

Disclaimer: SPI Supplies manufactures plasma etchers and supplies low acid
GMA.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


From daemon Thu Apr 26 05:41:19 2001



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Thu, 26 Apr 2001 06:34:39 -0400
Subject: Re: Replacement block holder for Sorvall

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Give Bill McGee a call. He operates Microtome Service Company out of
Liverpool, New York. He has lots of parts and provides excellent
service.

315-451-1404.

Dick Briggs
Smith College


From daemon Thu Apr 26 06:29:28 2001



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Thu, 26 Apr 2001 07:25:37 -0400
Subject: Thin sections/glass knives (summary-long)

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Thanks to all that responded to my question about making it easier
for students to cut thin sections using glass knives. Three key
points emerged: small block faces, fresh knives (changed often), and
a variety of different embedding media. A summary of the responses
is below.

Thank you again.

Dick Briggs
1. This is I think a quite common problem. The students in my lab
normally embed in Spurr's firm (both animal and plant tissue) and
they initially use glass knife and after they feel comfortable with
glass knife, I let them use a diamond knife to do their final
sectioning. We also use MT2 and MT2B ultramicrotomes.

2. I think LR White resin should fit the bill. It penetrates easily
and you don't have the same level of toxicity as with Spurr's (which
is always good for beginners).

3. Teaching microtomy is the most difficult portion of any
microscopy class. There is such a long learning curve and so many
pitfalls that getting good sections depends on the ultimate patience
and dexterity of the student. Some will get it right off, others
must persevere.
You probably already know my suggestions but I'll put my 2
cents in. My suggestion is to start the student with a quality block
of a material that cuts easily. Mouse or rat, kidney or liver are
good candidates. As they get good sections on these then move them
to tissues that may be more difficult. They may be able to
troubleshoot problems without questioning the block.
I would also ask how the knives are broken? It may be a dull
knife problem. A balanced break really helped improve the quality of
the knives we were getting from an LKB knife breaker. Additionally a
slow break has always seemed better. Wetting the scribe before
breaking also lessens the breaking force required and improves edges.
Of course small block faces are also going to improve the results
dramatically but trying to get the student to cut down the block face
is difficult. There are all the usual excuses.

4. I think some of the problem is your choice of tissue rather than
plastic. Plants have tough walls, and open spaces mixed together.
This causes a lot of different plasticities (?) within a section
because some areas will infiltrate to a better or worse degree than a
neighboring area. You would have much better luck using a
homogeneous mammalian tissue like liver.

5. I faced the same problem. I placed most of the blame on the
novices' inability to make good knives. My solution: I break about
25-50 knives a week and give them to the students. I have found that
it greatly enhances their ability to get good sections. I also got a
grant to buy a new ultramicrotome (Ultracut T). Between my knives
and the new 'tome, things are much better now.

6. I think that glass knives dull very rapidly (either damage or
plastic build-up on the edge), and so we have always tried to get the
first few sections at a given place on the knife edge, which tend to
be the best. I wrote a brief procedure for students years ago, based
on that approach, and have typed it below (the handout had a drawing
of a glass knife, with area A = 1/3 of edge on the side where the
whorl meets the edge, area B = middle
1/3 of knife edge, area C = 1/3 of edge at side where whorl is
farthest from the edge):

a. Face the block in area C of knife (see diagram). Cut semithin
sections (if desired) in area B of knife. Then move to area A.
b. Bring the block face parallel with the knife, using the shadow
method. Then use the shadow to bring the block face as close to the
knife (but without touching) as you dare.
c. With the ultramicrotome set for ultrathin sections, manually turn
the microtome wheel quite rapidly until you see the first sign of
contact (usually a sliver off one side of the block face), then stop
turning.
d. Turn on automatic sectioning at usual slow cutting speed. Cut
about6-12 full-face sections, then stop and pick them up on EM grids.
e. Retract the stage slightly, move laterally to another place in area A,
and repeat steps 2-4. Continue until area A has all been utilized (or
until you have all the sections you need).

7. I teach EM at a four-year college also. I use Spurr's and glass
knives made with an LBK 7800 knife maker. I am careful not to select
difficult tissues (nervous or muscle or bone or cartilage), but
generally my students have been able to get excellent sections by the
end of the semester.
a. Details to consider: Do your knife maker and microtome work properly (I
have an MT2-B and a newer Leica UltraCut. Some students actually
prefer the MT2-B. Are you fully dehydrating specimens and are you
using truly dry acetone to dehydrate--I dehydrate mine with ashed
CUS04.
b. Are you buying good float glass and are the students cleaning it fully and
handle it carefully. Mine wash with detergent and hot water, DI
water, and ethanol, and wash glass and make knives immediately before
use.

8. I always have good results using Spurr's. The sample morphology
is not as good as other resins. Be sure to polymerize for a good 48
hours or more.

9. I'm doing the same thing with my EM class this semester. I use
only Spurr's for both animal and plant tissues and glass knives (our
knife breaker is as old as our MT-2). I find students have greater
success with the smallest block faces. I also have them use
Formvar-coated grids (100 mesh) that tend to stabilize those sections
with bad knife marks. I start by making them get thick sections on
larger block faces -- they learn the mechanics of the microtomes,
block trimming, etc. When they are ready to get thin sections it
seems easy to them to just let the motor take over.

10. I typically use an Epon-Araldite mixture for all of my
biological samples (generally mammalian soft tissue samples) without
any sectioning problems over many years. For botanicals you might
need a low viscosity resin like Spurr's. I use MT-2B's in teaching of
my students and they rarely have problems obtaining good sections
(silver) in ribbons. The key I believe is in the block trimming. My
students trim their trapezoid faces no larger than 0.5mm on the
longest side (many are around 0.25 mm). I also have the students use
the flimsy double-edge razor blades vs. the single edge variety - a
trick I learned from my mentor. The blades are much sharper (and more
difficult to handle - I require my students to have Band-Aids on hand
for block trimming ;-) and give much smoother top and side of the
pyramid surfaces.
The Epon-Araldite Mixture I use is as follows:

Stock Solution (can be frozen)
Small Volume Large Volume
Araldite 6005 12.5ml 25ml
Poly/Bed 812 15.5ml 31ml
Dibutyl phthalate 2ml 4ml

Final Working Solution

Stock Solution 4ml 8ml
DDSA 10ml 20ml
DMP-30 14 drops*
28 drops*
(*Drops introduced with a Pasteur Pipette)
11. Centuries ago, I used the following mixture to embed cellular
slime molds for
thin-sectioning with glass knives:
Araldite 502 13.5 ml
DDSA 11.5 ml
DMP-30 0.4 ml
The Araldite made it a little softer and easier to cut. Hope it helps!

12. My recommendation is to use LR White on a tissue like spleen,
kidney, intestine or heart muscle. One mouse would give you more than
enough tissue to last a lifetime. Plants are notoriously difficult to
section (walls, air spaces, etc.). If you wish to avoid killing
animals, you might sacrifice one mouse and put away tissues in
fixative or buffer and then provide the students with vials
containing the tissues. Also, sometimes vivariums may be euthanizing
animals or a fish may die, etc. providing some valuable tissues.

13. When I was an undergraduate (eons ago) I used the "American
Araldite" recipe:

Araldite 502 27 parts
DDSA 23 parts
DMP-30 1.5-2.0% (accelerator)
I never included dibutylpthalate.

We mixed it in old (but clean) baby food jars by volume. We had a
"reference" jar with marks at the appropriate places and just lined
up the reference jar with the one we were measuring into. Very crude
but we got good results. I used small pieces of tissue (kidney,
spleen, sm. intestine) and went straight from 1:1 to pure resin and
into the oven. The mixture is very viscous but can be warmed a bit to
make handling easier. I don't know
if plant material would work. I used glass knives and an MT-1, but
not after a lot of coffee or a night on the town.

14. I have played this game for 32 years now and still don't
consider myself an expert because things fool me all the time.
I believe almost any embedding medium will work, if
instructions are followed and components are fresh. I use pre-mix
kits from TAAB, add bottle one to bottle two etc. It's for an easy
life. The leftovers, which can be most of the bottle, go into the
freezer. Fresh Spurr's cuts like a treat, sometimes thawed Spurr's
is fine, sometimes it is not. The reasons can be maybe a little
humidity gets in if not up to room temperature (maybe) or it begins
to age (increase viscosity due to starting to cure).
Anther things to remember are the smaller the block, the
better it cut? Also, don't dwell on one piece of edge for too long,
often I get just a couple of grids and then move along.
I used to make a special mix which was magic (really). It
was TAAB-Hard. With about 1% extra MNA (used to weigh all the
components) and 1% DMP-30 accelerator. Curing was also important -
48 hours at 35 degrees C, 24 at 45 and 24 at 60 degrees. It was very
hard and brittle. It was sterically inhibited i.e. not highly
cross-linked but with as much hardener (MNA) as ever allowed. It cut
all day on one knife and was terrific. But people didn't want to
follow the curing schedule - 4 days!! But the results were the best
sections I ever used to cut; I don't do so well these days, even with
diamonds.

15. I too teach an undergrad EM course (with Sorvall MT-2s). I've
discovered my students are very reluctant to change knives once
started. Forcing constant changes has improved the sectioning. We use
Spurr's:
10 ERL
5 DER
25 NSA
0.5 DMAE
These proportions work best. After dehydration through 50/75/80/95 we
go through 4 100% rinses then 1resion:2 ETOH for 3 hours, then 1ETOH
to 2 resins (2-3 hours) then full resin overnight - then bake. I
assume you're using these proportions so if you want more detail, let
me know. The strange thing is my kids do quite well at getting thins
with glass knives - they consistently surpass my expectations.

16. We us Epon with mouse liver. Spurr's would probably be better
with plants. Plants are always are a tough go for the uninitiated
(though probably easier to obtain and no animal right's issues).
Plants also require longer infiltration times and smaller increases
between infiltration steps (I usually go 25, 50, 70, 80, 90, and a
couple of 100% before polymerization).
LR White has been suggested, but as the resin is more hydrophilic it
is actually more difficult to learn on at first. The block face has a
tendency to wet easily and usually students get frustrated trying to
figure out why the block face is getting wet even with the more
hydrophobic resins like Spurr's or Epon.

17. I find the same problem. I also find that having them get very
close to really mastering thick sectioning first (thereby seeing some
really pretty LM slides) greatly helps their confidence, and I also
have them use the tiniest faces they can manage (0.1mm?) to get
started with thins. They must be the correct shape with no ragged
edges (such as you might get after taking (or trying to take) many
thicks. Also make sure their knives are good - especially FRESH - and
that they are using the correct edge and don't' try to take too many
sections before switching knives. Have them start with something
like liver where the tissue/resin interface is not an issue. I have
tried Spurr's but it is so nasty to use, and is not as stable or as
pretty in the EM.

Secondarily, I keep troubleshooting sheets taped to the wall - check size!
Check shape! Check knife-edge! Check speed! Check angle! etc. I find this
helps beginners understand that lots of factors need to be satisfied - it
seems that so many of them probably get by in other courses by cramming the
night before, which just doesn't' work here... although some of the
'rules' (such as face size) can be relaxed once they get the basics down.

18. There is a resin additive sold by Electron Microscopy Sciences,
called "Sure-Cut Surfactant" (their #21630). I have not tried it
myself, but remember a posting on this server 1-2 years ago praising
this additive very highly. The guy was saying it saved his students a
lot of frustration. There is a trade-off, of course, -- they say this
additive somewhat interferes with staining. I would give EMS a call;
they are usually helpful. (I have no interest in their sales, of
course.)

19. Is it the embeddment or sectioning which is the problem? For
sectioning try to keep the section area as small as possible. If you
keep the sectioning width less than ~.25mm it makes sectioning much
easier.

20. There is no magic embedding media that allows easy sectioning
with glass knives. All they can do is trim the blocks as small as
possible, and I do mean small. If the block face looks big under the
high-end magnification of the stereomicroscope it is still too large.
Have them practice (a lot) trimming blocks. Don't them rush into
thick sections too soon. Make sure they can master the cutting
one-micron sections and them trimming to the desired area. The guy I
trained under had no clue. He had us cutting as soon as possible. The
drop rate for the class was around 50%. And console them even the
big folks had to learn using glass knives. It is possible to do. If
they think that is hard wait till they stain the sections with
Reynolds lead for the first few hundred times. Tell them to cheer up;
at least they are not learning on an MT1 manual microtome like I did.

21. Is your course specifically in electron microscopy or would
slightly thicker sectioning be almost as good from the microtechnical
perspective? For light microscopy, I embed plant tissue in Glycol
Methacrylate (GMA) from Electron Microscopy Sciences. The embedding
protocol is simpler, and there is the potential that the plastic is
less carcinogenic than Spurr's resin. I have recently completed a
project in which I was sectioning largish block faces of plant apices
embedded in GMA using a glass knife on an Olympus rotary microtome.
Sections of less than 1 micrometer were no sweat and I bet they'd be
even easier on an ultramicrotome. Very satisfying from the teaching
side as it should keep the student frustration level down. GMA is
also quite easy to stain with e.g. Richardson's stain or methylene
blue. Unfortunately, GMA is a bit too soft to section thinner and it
doesn't hold up well under the e-beam so for electron microscopy I
use Spurr's or LR White.

22. I noticed your comments on the Microscopy ListServer about
students and thin sections. There are a couple of fairly easy ways
to make it less painful. My old graduate advisor and I have worked
together for many years and I have watched, and at times helped him
teach his EM course. For his students sectioning was always the
worst experience. Two things might help: 1) use a soft to very soft
formulation of one of the Epon substitutes. In that way trimming and
thick sectioning are easier and glass knives do not dull as quickly
nor do they need to be perfect. The second is make a small block
face. Unfortunately that may be more difficult to do than
sectioning. Some friends of mine at UC Davis use the soft
formulation of Epon which really makes sectioning and trimming
easier. It is soft enough to easily make an imprint with a
fingernail.

23. I was a former student at San Francisco State University (I've
graduated in May 2000), and I took an electron microscopy course at
that university. Back to my electron microscopy course. During part
of that course, I was supposed to section some embedded tissue of my
choice (heart / liver /? (can't recall off-hand)), and I had
considerable trouble using a microtome well. As I recall, we had
some Sorvalls available for use, but I'm unsure if they were MT-2s.
I prepared all my tissues (and practice, plastic "pellets") by first
cutting with straight razor blades to make an acceptable-sized
pyramid, then continue by sectioning with a microtome.
Glass knives were used on these embedded tissues -- the plastic used
was Epon -- and I recall that one major problem I had was in making
sure that the microtome sectioned at a consistent speed. In addition,
the glass knife edges would dull pretty quickly after a small number
of sections were made, and so it became imperative to use several
glass knives for any particular embedded material. I eventually was
able to section pretty well, but I also
picked up sections from below with a grid (of 200 mesh) that I bent
rather badly during the collection process.
Epon worked OK for sectioning, and while it's true that students can
indeed get discouraged, I also know from experience that students
have very different aptitudes towards handling microtomes, preparing
the pyramids, etc.
I hope that my experience with sectioning may help you understand
better (from a student's perspective) the various trouble areas that
could occur when students are first learning how to section embedded
material.



From daemon Thu Apr 26 06:49:46 2001



From: l.tetley :      l.tetley-at-bio.gla.ac.uk
Date: Thu, 26 Apr 2001 12:44:31 +0100
Subject: Developments in Energy Filtered Electron Microscopy - Ist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



********************* 1st Announcement and call for contributions
*****************************

A meeting organised by the Royal Microscopical Society and supported by
EMAG, FEI UK Ltd, JEOL (UK) Ltd.,LEO EM Inc., Gatan UK, TVIPS GmbH

DEVELOPMENTS IN ENERGY-FILTERED ELECTRON MICROSCOPY

Wednesday 4 July 2001

Department of Materials, Oxford University

The use of electron energy filters in analytical electron microscopy
(EFTEM) is a relatively recent development that is proving to be an
extremely powerful tool. In-column filters (e.g. so-called "omega-filters")
and post-column filters (e.g. the "GIF") represent different approaches
which have their own advantages as well as experimental difficulties. This
one day meeting is designed to explore the current state of the art, both
with regard to the instrumentation and also applications of EFTEM in life
and physical sciences.

Invited speakers:
Dr Bernd Feja (Tietz Video & Image Processing Systems GbmH)
Energy-filtered electron tomography
Prof Joachim Mayer (Aachen University of Technology)
EFTEM - the state of the art and future trends
Dr Paul Midgley (University of Cambridge)
EFTEM image series - taking elemental mapping into a new dimension
Prof Michael Trendelenburg (German Cancer Research Center, DKFZ)
EFTEM in biomedicine & biotechnology: Recent advances in specific
element mapping

* CONTRIBUTIONS
Contributed presentations are now being sought. Abstracts of no more than
200 words should
be sent by email to: crispin.hetherington-at-materials.ox.ac.uk and should
arrive by 31 May, 2001.

* REGISTRATION
Registration will be £30 RMS/EMAG members, £15 students and £40 others and
a printable form will be avialable from the new RMS website, from May on
this page :

http://www.rms.org.uk/current%20events.html#eftem

Further details are available from the organisers:
Dr Crispin Hetherington, tel. 01865 273799,
crispin.hetherington-at-materials.ox.ac.uk
Dr Laurence Tetley, tel. 0141 330 4431, l.tetley-at-bio.gla.ac.uk
and from the meeting website :
http://www-em.materials.ox.ac.uk/events/eftem.html

Note this meeting is timed to follow the 1-day FEGTEM III meeting to be
held on 3 July 2001, also in Oxford
(http://www-em.materials.ox.ac.uk/events/fegtem.html)
****************************************************************************
****************

Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel/FAX 0141 330 4431

Integrated Microscopy Facility:
http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


From daemon Thu Apr 26 06:58:37 2001



From: JHoffpa464-at-aol.com
Date: Thu, 26 Apr 2001 07:54:39 EDT
Subject: araldite 502

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--part1_6f.1457c871.2819667f_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

OK this is going to seem like a stupid question. I am going to try araldite
502 embedding media. the instruction sheet calls for a volumetric
measurement. I would prefer to measure the each chemical in a balance. OK
here is the question: do i multiply the specific gravity by the volume or
divide it. it has been a long time for me since chemistry 101.
thanks

--part1_6f.1457c871.2819667f_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} OK this is going to seem like a stupid question. I am going to try araldite
{BR} 502 embedding media.  the instruction sheet calls for a volumetric
{BR} measurement. I would prefer to measure the each chemical in a balance. OK
{BR} here is the question: do i multiply the specific gravity by the volume or
{BR} divide it. it has been a long time for me since chemistry 101.
{BR} thanks {/FONT} {/HTML}

--part1_6f.1457c871.2819667f_boundary--


From daemon Thu Apr 26 07:00:49 2001



From: Howard Mulhern :      howard.mulhern-at-TCH.Harvard.edu
Date: Thu, 26 Apr 2001 07:52:00 -0400
Subject: Bx Turn Around Time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If I'm not swamped, which is never, I do a two day processing. Using a
Lynx overnight to get into resin I then put the specimens into molds and
let them cure for 24 hours. When I come in on day three I can cut thick
sections, have a conference with the Doc who submitted the case then cut
and stain the thins and I'm ready to sit down at the scope.
In reality, A specimen coming into our cutting room is submitted for
routine light microscopy and possibility special stains. Those sections
are then reviewed and the decision is made to submit the case for EM
examination. That can take one or two days. So there are four,
possibility five days before I can photograph the case. Another issue is
prioritization. I do live patients first and I do the tumors before
genetics cases. It makes no sense to work on a possible mitochondria
disorder when there is a kid with a chest wall tumor or a brain tumor.
The same goes for a kidney biopsy over a ciliary disorder. I have to
prioritize and frequently cases get put off a few days until the heats
down. With a current volume of about four hundred cases a year and an
additional two hundred cases for research I'm often asked "How long will
this take?" My usual answer which includes the scoping and printing or
making a CD is five working days. Autopsy's are not high on my list and
periodically I'll hear that the resident who submitted the case has
rotated into another department before the EM is done.
To answer your question I'd have to know what your case load is and how
many hands are available.

Howard Mulhern
Supv. Path. EM Facility
Children's Hospital
Dept. of Pathology
300 Longwood Ave.
Boston, 02115 Ma.



From daemon Thu Apr 26 07:33:47 2001



From: l.tetley :      l.tetley-at-bio.gla.ac.uk
Date: Thu, 26 Apr 2001 13:29:21 +0100
Subject: Developments in Energy Filtered Electron Microscopy - Ist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



********************* 1st Announcement and call for contributions
*****************************

A meeting organised by the Royal Microscopical Society and supported by
EMAG, FEI UK Ltd, JEOL (UK) Ltd.,LEO EM Inc., Gatan UK, TVIPS GmbH

DEVELOPMENTS IN ENERGY-FILTERED ELECTRON MICROSCOPY

Wednesday 4 July 2001

Department of Materials, Oxford University

The use of electron energy filters in analytical electron microscopy
(EFTEM) is a relatively recent development that is proving to be an
extremely powerful tool. In-column filters (e.g. so-called "omega-filters")
and post-column filters (e.g. the "GIF") represent different approaches
which have their own advantages as well as experimental difficulties. This
one day meeting is designed to explore the current state of the art, both
with regard to the instrumentation and also applications of EFTEM in life
and physical sciences.

Invited speakers:
Dr Bernd Feja (Tietz Video & Image Processing Systems GbmH)
Energy-filtered electron tomography
Prof Joachim Mayer (Aachen University of Technology)
EFTEM - the state of the art and future trends
Dr Paul Midgley (University of Cambridge)
EFTEM image series - taking elemental mapping into a new dimension
Prof Michael Trendelenburg (German Cancer Research Center, DKFZ)
EFTEM in biomedicine & biotechnology: Recent advances in specific
element mapping

* CONTRIBUTIONS
Contributed presentations are now being sought. Abstracts of no more than
200 words should
be sent by email to: crispin.hetherington-at-materials.ox.ac.uk and should
arrive by 31 May, 2001.

* REGISTRATION
Registration will be £30 RMS/EMAG members, £15 students and £40 others and
a printable form will be avialable from the new RMS website, from May on
this page :

http://www.rms.org.uk/current%20events.html#eftem

Further details are available from the organisers:
Dr Crispin Hetherington, tel. 01865 273799,
crispin.hetherington-at-materials.ox.ac.uk
Dr Laurence Tetley, tel. 0141 330 4431, l.tetley-at-bio.gla.ac.uk
and from the meeting website :
http://www-em.materials.ox.ac.uk/events/eftem.html

Note this meeting is timed to follow the 1-day FEGTEM III meeting to be
held on 3 July 2001, also in Oxford
(http://www-em.materials.ox.ac.uk/events/fegtem.html)
****************************************************************************
****************

Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel/FAX 0141 330 4431

Integrated Microscopy Facility:
http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


From daemon Thu Apr 26 08:21:31 2001



From: l.tetley :      l.tetley-at-bio.gla.ac.uk
Date: Thu, 26 Apr 2001 14:17:27 +0100
Subject: Developments in Energy Filtered Electron Microscopy - Ist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



********************* 1st Announcement and call for contributions
*****************************

A meeting organised by the Royal Microscopical Society and supported by
EMAG, FEI UK Ltd, JEOL (UK) Ltd.,LEO EM Inc., Gatan UK, TVIPS GmbH

DEVELOPMENTS IN ENERGY-FILTERED ELECTRON MICROSCOPY

Wednesday 4 July 2001

Department of Materials, Oxford University

The use of electron energy filters in analytical electron microscopy
(EFTEM) is a relatively recent development that is proving to be an
extremely powerful tool. In-column filters (e.g. so-called "omega-filters")
and post-column filters (e.g. the "GIF") represent different approaches
which have their own advantages as well as experimental difficulties. This
one day meeting is designed to explore the current state of the art, both
with regard to the instrumentation and also applications of EFTEM in life
and physical sciences.

Invited speakers:
Dr Bernd Feja (Tietz Video & Image Processing Systems GbmH)
Energy-filtered electron tomography
Prof Joachim Mayer (Aachen University of Technology)
EFTEM - the state of the art and future trends
Dr Paul Midgley (University of Cambridge)
EFTEM image series - taking elemental mapping into a new dimension
Prof Michael Trendelenburg (German Cancer Research Center, DKFZ)
EFTEM in biomedicine & biotechnology: Recent advances in specific
element mapping

* CONTRIBUTIONS
Contributed presentations are now being sought. Abstracts of no more than
200 words should
be sent by email to: crispin.hetherington-at-materials.ox.ac.uk and should
arrive by 31 May, 2001.

* REGISTRATION
Registration will be £30 RMS/EMAG members, £15 students and £40 others and
a printable form will be avialable from the new RMS website, from May on
this page :

http://www.rms.org.uk/current%20events.html#eftem

Further details are available from the organisers:
Dr Crispin Hetherington, tel. 01865 273799,
crispin.hetherington-at-materials.ox.ac.uk
Dr Laurence Tetley, tel. 0141 330 4431, l.tetley-at-bio.gla.ac.uk
and from the meeting website :
http://www-em.materials.ox.ac.uk/events/eftem.html

Note this meeting is timed to follow the 1-day FEGTEM III meeting to be
held on 3 July 2001, also in Oxford
(http://www-em.materials.ox.ac.uk/events/fegtem.html)
****************************************************************************
****************

Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel/FAX 0141 330 4431

Integrated Microscopy Facility:
http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


From daemon Thu Apr 26 08:21:32 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 26 Apr 2001 09:16:18 -0400
Subject: Re: Thin sections/glass knives (summary-long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One thing that was not mentioned was the use of resin conditioners
that help to preserve the knife edge of a glass knife. We use this with
beginners. We have used lecithin and "PolyCut Ease" from Polysciences
Information on this from a previous discussion can be found at
http://www.biotech.ufl.edu/icbr/emcl/db/slippery.html
I am not sure if a conditioner for LR White is mentioned there or
in the Mollenhauer pub., but in my personal conversations with Hilton, he
said that camphor could be used for acrylic resins. If this is of any
interest I can dig deeper into this or perhaps we can contact Hilton
Mollenhauer directly.

At 07:25 AM 4/26/2001 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Greg Erdos
Assistant Director
Biotechnology Program Ph. 352-392-1295
University of Florida Fax 352-846-0251
PO Box 118525
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Thu Apr 26 08:24:06 2001



From: Howard Mulhern :      howard.mulhern-at-TCH.Harvard.edu
Date: Thu, 26 Apr 2001 09:15:06 -0400
Subject: Clinical Bx Turn Around Time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} If I'm not swamped, which is never, I do a two day processing. Using
} a Lynx overnight to get into resin. I then put the specimens into molds and
} let them cure for 24 hours. When I come in on day three I can cut thick
} sections, have a conference with the Doc who submitted the case then cut
} and stain the thins and then I'm ready to sit down at the scope.

} In reality, A specimen coming into our cutting room is submitted for
} routine light microscopy and possibility special stains. Those sections are
} then reviewed and the decision is made to submit the case for EM
} examination. That can take one or two days. So there are four, possibility
} five days before I can photograph the case. Another issue is
} prioritization. I do live patients first and I do the tumors before
} genetics cases. It makes no sense to work on a possible mitochondria
} disorder when there is a kid with a chest wall tumor or a brain tumor. The
} same goes for a kidney biopsy over a ciliary disorder. I have to prioritize
} and frequently cases get put off a few days until the heats down. With a
} current volume of about four hundred cases a year and an additional two
} hundred cases for research I'm often asked "How long will this take?" My
} usual answer which includes the scoping and printing or making a CD is five
} working days. Autopsy's are not high on my list and periodically I'll hear
} that the resident who submitted the case has rotated into another
} department before the EM is done. To answer your question I'd have to know
} what your case load is and how many skilled hands are available.

} Howard Mulhern
} Supv. Path. EM Facility
} Children's Hospital
} Dept. of Pathology
} 300 Longwood Ave.
} Boston, 02115 Ma.
}
}
}
}



From daemon Thu Apr 26 08:51:54 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Thu, 26 Apr 2001 09:51:22 -0400
Subject: Re: TEM: tools for picking up ultrathin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been using a Chien grid to pick up sections, like a loop, and place it on top of a coated grid. Let water evaporate and sections will drop on to the grid below.
I dip Chien grids in 1% formvar, shake off excess and place then on a filter paper to dry. This treatment ensures water film is maintained during the transfer. Cleaning the Chien grids in acid has the same effect. The handle of Chien grid is bended for better handling.
I have tried a "perfect loop" and I prefer Chien grids.





Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Claudia Hayward-Costa {LS_S562-at-crystal.kingston.ac.uk} 04/25 5:57 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Microscopists,

I wondered how commonly people use a tool (like a loop) to pick up
ultrathin sections.

Does it make life easier or does it only introduce different problems?

I was told that I can buy some "luxury" - but am still undecided.

Your views would be very much appreciated.

Regards

Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk
Fax: 44(0)208 547 7562




From daemon Thu Apr 26 08:52:09 2001



From: JENKINS-at-afip.osd.mil
Date: Thu, 26 Apr 2001 09:42:39 -0400
Subject: RE: Apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I also enjoyed the joke, as did my colleagues.

Marie

} -----Original Message-----
} From: Earl Weltmer [SMTP:eweltmer-at-home.com]
} Sent: Wednesday, April 25, 2001 8:19 PM
} To: Chuck Butterick; Microscopy-at-sparc5.microscopy.com
} Subject: Re: Apology
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I rather enjoyed it.
}
} Earl
}
} ----- Original Message -----
} } From: "Chuck Butterick" {cbutte-at-ameripol.com}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, April 25, 2001 5:36 AM
} Subject: Apology
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Listers,
} }
} } A slip of the finger caused me to send an un-topical joke to all.
} } While no has yet criticized (though some have expressed
} appreciation
} } and/or agreement), I feel that the joke was inappropriate to the
} } venue. My apologies.
} }
} } Chuck Butterick
} }
} }
}


From daemon Thu Apr 26 09:36:17 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 26 Apr 2001 10:31:21 -0400
Subject: Re: araldite 502

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







OK this is going to seem like a stupid question. I am going to try araldite
502 embedding media. the instruction sheet calls for a volumetric
measurement. I would prefer to measure the each chemical in a balance. OK
here is the question: do i multiply the specific gravity by the volume or
divide it. it has been a long time for me since chemistry 101.
thanks

Dear J,
At the beginning of every semester I give three general hints on
calculations to my students. The relevant one for you is always write down
units explicitly. Since the density (numerically equal to the specific gravity
in cgs units) is in g/cm^3, if you multiply the volume (in cm^3) by the density,
you get the mass.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Thu Apr 26 10:11:32 2001



From: Haixin Xu :      xu-at-umbc.edu
Date: Thu, 26 Apr 2001 11:07:17 -0400
Subject: Used ultramicrotome?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Microscopists,

Does somebody there have an used, but working ultramicrotome for sale? I
am looking for such a microtome.


Haixin Xu

University of Maryland, Baltimore County

phone: 410-455-2296



From daemon Thu Apr 26 11:23:50 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Thu, 26 Apr 2001 12:17:14 -0400
Subject: TEM negative holders for the Polaroid SprintScan 45 Ultra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

Following the recent discussion thread of scanners, I wanted to say that we are the proud owners of a Polaroid SprintScan 45 Ultra, and are very happy with the results we have gotten from it. It came with a number of negative holders covering a range of sizes from 35mm to 4x5, except one for 3.25x4.25 TEM sheet film. Right now we're trying to think of ways around this problem and would be happy to hear offline from anyone who has some ideas and/or solutions. I would even entertain the idea of having a holder made (this is not an option locally for me).

Thanks in advance for any help (and there is usually plenty, thanks to this list!).

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Apr 26 11:53:18 2001



From: Eric :      biology-at-ucla.edu
Date: Thu, 26 Apr 2001 09:49:07 -0700
Subject: Re: Bx Turn Around Time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here in the lab we still do the hand processing.. not automated processor yet.

There are two techs, but turnaround time is different between both of us..
i.e. one of us works faster than the other...

Normally when a biopsy comes in on say Monday.. I can have images by
Wednesday.. Out here we do not use a darkroom anymore.. Our lab has gone
digital with a AMT system.

The Pathologists are thrilled with it since it cuts down our turnaround
time from 5 days to 3 days...


Eric
UCLA Medical Center


From daemon Thu Apr 26 15:26:36 2001



From: Leonardo Lagoeiro :      lagoeiro-at-degeo.ufop.br
Date: Thu, 26 Apr 2001 17:19:52 -0300
Subject: Universal_Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopist,

Does anyone knows where I can find a five-axes (four-axes U-Stage is
fine too) universal stage for sale or donation?

Thank all of you in advance.

Best wishes,

Leonardo
--
---
Leonardo Lagoeiro
Departamento de Geologia
Universidade Federal de Ouro Preto
Ouro Preto, MG, 35400-000
Brazil
E-mai: lagoeiro-at-degeo.ufop.br


From daemon Thu Apr 26 15:35:53 2001



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Thu, 26 Apr 2001 14:27:53 -0500
Subject: carbon black in polymer

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Does carbon black cause a problem in the TEM? What percautions should I
take? Is the use of the cold finger recommended?


From daemon Thu Apr 26 15:46:49 2001



From: Robert Fitton :      fittonro-at-luther.edu
Date: Thu, 26 Apr 2001 14:40:41 -0600
Subject: Undergraduate EM courses

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I would like to start up a thread about the level of instruction and the
amount of time that undergraduates spend in an introductory EM course that
covers TEM & SEM. The need comes from low numbers of student credit hours
generated verses the amount of time spent by the students and instructor in
the course. For example, I usually have between 2 to 4 students in my four
credit hour course, taught every fall sememster. The students receive two
one hour lectures per week, two supervised two hour labs and a third
unsupervised two hour lab. I teach the students in pairs during the
intensive hands-on portions like ultramicrotomy and scope operations, so I
usually have two lab sections to prep for, or about eight contact hours for
labs per week. With the lectures, I then have ten hours of contact per
week for the course.

What are the teaching loads and class sizes of other instructors who teach
undergrad EM?

Thanks

Robert


Robert Fitton
Teaching Associate/Director of Laboratories
Luther College
Department of Biology
700 College Drive
Decorah, IA 52101

Voice 563-387-1559
FAX 563-387-1080
(If the 563 area code does not work, try our old area code of 319)

Enjoy a visit to our website: http://www.luther.edu/~biodept/




From daemon Thu Apr 26 16:10:20 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 26 Apr 2001 16:59:19 -0400 (EDT)
Subject: Turn around time (TAT)

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Our TAT differs depending on the service rendered.

Diagnostic virology: 15 min to ~2 hours (depending on whether we
ultracentrifuge) for negative staining of bodily fluids. 2-3 days
for thin sections of tissue (depending on how many blocks we have to cut
and how long we have to look if the sample shows no viruses before we
call it negative). Three super techs do ~700 negative stain specimens and
100 thin section samples of clinical material per year, plus a number of
research samples (20-40, some of which are cryosections for IEM).

Surgical pathology: 2 days (about 32 hours). In on a morning, into the
oven that night, cut, photographed, and dark room micrographs printed
and delivered to the pathologist by 5 PM the next day. (We're working
on going digital.) Two super techs do ~500 clinical samples plus ~100
research samples a year.

Five techs total. They can cross cover, but each has a specialty, and
generally, the same 3 do virology, and the other 2 do surgical pathology.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Apr 26 16:27:42 2001



From: Leonardo Lagoeiro :      lagoeiro-at-degeo.ufop.br
Date: Thu, 26 Apr 2001 18:26:53 -0300
Subject: Re: Universal_Stage

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} Dear Microscopist,

} Does anyone knows where I can find a five-axes (four-axes U-Stage is
} fine too) universal stage for sale or donation?

} Thank all of you in advance.

} Best wishes,

} Leonardo
} --
} ---
} Leonardo Lagoeiro
} Departamento de Geologia
} Universidade Federal de Ouro Preto
} Ouro Preto, MG, 35400-000
} Brazil
} E-mai: lagoeiro-at-degeo.ufop.br

The message you sent to jeolbxl-at-pophost.eunet.be
could not be delivered.
Either you misspelled your correspondent's name, or
jeolbxl no longer exists in the pophost.eunet.be domain.

If you want more information, you can contact the postmaster
at postmaster-at-pophost.eunet.be. Please understand that we can not give
emailaddresses from our customers.

Kind Regards,

The mail delivery system.
--
---
Leonardo Lagoeiro
Departamento de Geologia
Universidade Federal de Ouro Preto
Ouro Preto, MG, 35400-000
Brazil
E-mai: lagoeiro-at-degeo.ufop.br


From daemon Thu Apr 26 18:10:55 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 26 Apr 2001 18:48:51 -0400
Subject: TEM negative holders for the Polaroid SprintScan 45 Ultra

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Polaroid has a small sheet insert that fits into the 4 X 5 and holds the negative down with small magnets. You need to call someone at Polaroid and request this. They gave them to me and a few others on the List for free. I originally talked to John Warren WARRENJ1-at-POLAROID.COM There is also a Dennis Lizier, but I do not have his contact information.
-Scott

-----Original Message-----
} From: Paula Allan-Wojtas [mailto:AllanWojtasP-at-em.agr.ca]
Sent: Thursday, April 26, 2001 12:17 PM
To: microscopy-at-sparc5.microscopy.com


Hi, all,

Following the recent discussion thread of scanners, I wanted to say that we are the proud owners of a Polaroid SprintScan 45 Ultra, and are very happy with the results we have gotten from it. It came with a number of negative holders covering a range of sizes from 35mm to 4x5, except one for 3.25x4.25 TEM sheet film. Right now we're trying to think of ways around this problem and would be happy to hear offline from anyone who has some ideas and/or solutions. I would even entertain the idea of having a holder made (this is not an option locally for me).

Thanks in advance for any help (and there is usually plenty, thanks to this list!).

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Apr 26 18:11:49 2001



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 26 Apr 2001 18:23:15 -0500
Subject: Re: Film Processing and dynamic range and Exposure of film to

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Robert
Any undergraduate teaching of EM techniques we do strictly within the
context of student projects, chiefly in the final honours year (4th
year of the BSc course at Edinburgh). Since I joined the Botany
department staff in 1976 the policy has been not to teach methods. I
don't especially agree with that policy, but it is the way things have
always been done here. I get the luxury of a 1-hour presentation on EM
to first year biology students at the end of their first year. We also
demonstrate the EMs to class groups from various departments, and we
train a few students per year as they require access to EM for project
work, but there have been no formal taught undergraduate courses with
hands-on practical tuition in EM in the Science Faculty at Edinburgh
for many years now.
Chris

----- Original Message -----
} From: "Robert Fitton" {fittonro-at-luther.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, April 26, 2001 9:40 PM



FW: Re: Film Processing and dynamic range and Exposure of film to
electrons


} A couple of thoughts on film:
} Kodak special publication P-116 describes the interaction of electrons
with film (from the point of view of people that produce the film-I assume
they study it fairly closely):
} "Each electron in an incident beam may pass through perhaps 30 or
more silver halide grains before it is stopped completely...........The
trajectory is a changing one as collisions occur, and energy is lost to the
silver halide grains, and to a lesser extent, to the gelatin in which the
silver halide grains are dispersed. This transfer of energy, which is low
at the beginning of an electron path and increases as the electron is slowed
by collisions, is responsible for the formation of specs of silver atoms in
the affected grain. If the aggregate of silver atoms formed is sufficiently
large-believed to be between 3 and 6 atoms, the entire grain will be capable
of being converted to metallic silver during photographic development. While
the passage of a single electron may not render each grain developable, to
which it gives up energy, overall sensitivity is such that normally at least
one grain, and very likely, a number of grains, will be converted to
metallic silver. If this is the case, the photographic material records all
of the information in the electron beam;"
} This document further discusses contrast, illustrating the
sensitivity of the film with log exposure/density curves, but only up to a
density of 2. It may be worth noting that John Spence states in the appendix
of his text that for photographic emulsions, the exposure/density curve is
linear up to a density of between one and 2. The departure from linearity
would seem to be related to some sort of "pile-up" effect. While the
exposure/density curves may be linear over this range, the read-out of the
film is logarithmic, as the density is defined as the log base 10 of the
transmitted light intensity divided by the incident light intensity. That is
why, for example, one commonly used, and successful method of increasing
contrast is to increase the exposure level of the film. For example, a 10%
change in electron dose near a density of 1 will produce a 25% change in
transmitted light intensity, where a 10 % change in dose near a density of 2
will produce a 58% change in transmitted light intensity. This logarithmic
read-out characteristic is perhaps why all of the Kodak sensitivity plots
are in log dose/density format, and they are curves. Kodak also illustrates
how the contrast of the print can be estimated from the slope of these log
dose/density curves, since the slope increases with average density. In
summary, even though the density of the film may be linear with electron
dose, what you see in a print, what you get from a scanner, even what you
see from observing the film on a light box, is logarithmic with respect to
electron dose. This is also why images obtained with a CCD camera or an
image plate, which are linear with electron dose, have a distinctly
different appearance than images obtained with film unless digital gamma is
used to adjust the display. So, everyone was essentially correct in what
they said, it's just that they were not describing the same thing.
} One may want to look at the claims made for Fuji image plates on
their web site, which can be accessed at "fujimed.com/sub/FDL5000.pdf". They
show plots of log readout intensity/log dose for the Fuji image plate
superimposed on density/log dose for film. The Fuji image plate is more
linear than film, but for the range 0-2, film is relatively parallel to the
image plate. Beyond a density of 2, the nonlinearity becomes gradually more
pronounced, but there is never any saturation encountered.
} Finally, I would like to reiterate that this has been a fascinating
string, and it seems that everyone has contributed some useful and thought
provoking information.}
}
} John Mardinly
} Intel Materials Technology


From daemon Fri Apr 27 01:03:45 2001



From: Dr Deborah Stenzel :      d.stenzel-at-qut.edu.au
Date: Fri, 27 Apr 2001 16:08:08 +1000
Subject: re Undergraduate EM courses

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Dear Robert and all

I have the pleasure of teaching full semester (13 week) EM course to
undergraduates at Queensland University of Technology in Brisbane,
Australia. We've offered this course for quite a number of years - in
fact, even had it extended from part of a broader topic to a full semester
just for EM! The course is offered as part of the School of Life Sciences
undergraduate program and, hence, primarily addresses biological
EM. However, it is open to students from other disciplines - presently we
do not have a full EM course in the physical sciences, although my
colleague Dr Thor Bostrom teaches several weeks of EM in various courses
for physics, chemistry and engineering undergraduate students.

Apart from this, I will usually run single 2 hour practical sessions for a
couple of other undergraduate courses (mainly microbiology, where students
look at negatively stained microbes they have isolated from various
sources). I'll often be asked to give a half hour (sometimes an hour)
introductory EM lecture to these students, prior to the practicals.

I also teach a brief introduction (2-3 weeks) to EM as part of a
post-graduate course on cell structure and function in pathology. Other
post-graduates needing EM as part of their research are taught individually
by staff in our EM Facility, to the level that their projects require.


My full semester EM course is offered in the third year of the B. Applied
Science course. This year I have 30 students - the numbers have been
increasing over the past few years from a previous average of 20. I
usually also have a couple of post-graduate (research) students attending
the lectures - these students will be using our EM facilities as part of
their research, and I find this is an effective means of giving them some
useful background and theory to support their laboratory work.

For this course, I am allocated two by one hour lectures each week (total
of 26 hours lectures for the semester). I cover basic instrumentation
(TEM, SEM, preparation equipment) and biological sample preparation
methods, including cryotechniques. I also include immunolabelling methods,
some cytochemistry, microanalysis (Thor Bostrom gives these lectures), a
few interesting "non-biological" applications, and a brief introduction to
some other imaging methods (confocal, AFM etc).

Practical sessions have been a bit of a "challenge" as student numbers have
increased and EM staff numbers have decreased! However, I've now got a
system that works, and doesn't cause me (or the equipment) too much
stress. I have two by 3 hours practical sessions running each week - ie. 6
hours of my teaching time per week (total of 72 hours of my teaching time
for the semester).

Each student attends a total of 24 hours practical sessions for the
semester (this fits with our "official" allocation for total practical time
for a course of this credit point value). I divide the class into small
groups - 3 to 4 students per group, with current student numbers. For
preparation lab based pracs (where we have enough space and the students
don't need such close supervision), I'll have a number of these small
groups attending the same practical session. For EM operation and viewing
pracs, I'll only have one small group attending at a time (usually 1 or 1.5
hours of the session for each small group). This has enabled me to run
practical sessions almost single-handed - money to employ
tutors/demonstrators or other teaching assistants has been a bit difficult
to obtain! If anyone would like further information on the practicals I
run or on the logistics of this, please contact me by direct email - does
anyone else out there run EM practicals for undergraduates??? I haven't
seen a rush of emails in response to Robert's questions.

Fortunately, my university is still keen to have students doing "hands-on"
practicals in their courses. However, I've certainly had to decrease
practical work substantially since I first started teaching in this EM
course. A lot of this is due to decreased staff numbers in our EM Facility
- a problem that I guess most of us face. I teach other courses
(microbiology, parasitology) and encounter the same problems there - it is
now very difficult to get competent, experienced people to assist in
practical classes, particularly for more advanced or specialised courses.

On the bright side, I've found the students enjoy EM a great deal - and it
is one of the few opportunities our undergraduates get to use expensive and
(relatively!) modern instrumentation, even if it is only on a very limited
scale. I've not had many students who don't show a great deal of
enthusiasm when told it is now their turn to operate the TEM or SEM and
take some micrographs. This semester, more so than previously, at the end
of each practical session quite a number of my students have taken the time
to thank me for running the practicals (even after the night classes which
finish at 8pm). I figure that has got to be a good sign!!!


Regards
Deb
*****************************************************
Dr Deborah Stenzel
Lecturer (Microbiology)
School of Life Sciences
and
Applications Specialist (Biological)
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

Phone + 61 7 3864 5036
Fax + 61 7 3864 5100
email d.stenzel-at-qut.edu.au

http://www.sci.qut.edu.au/aemf



From daemon Fri Apr 27 04:19:21 2001



From: Yuan Wu :      wy-at-istec.or.jp
Date: 27 Apr 01 17:22:01 -0800
Subject: about simulation

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Hi, everyone,
Now, I need to simulate HRTEM image of a phase that I can not find its space group in X-ray powder diffraction files. Do you know whether it is possible for me to find its atomic positions. Could you please give me suggestions?
Thank you very much in advance.
WY




From daemon Fri Apr 27 05:10:42 2001



From: Andrew Chuvilin :      andrew_chuvilin-at-mail.ru
Date: Fri, 27 Apr 2001 14:05:29 +0400
Subject: TEM, SEM: educational software

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Dear Listers,
We are in need to prepare a short course on EM basics. I would be thankfull for any information about free internet resources or commercially availiable CDs containing illustative materials, animations, programms illustrating image formation (not simulating software) that we can use in the lectures and during the seminars.

Andrew


Dr. A.Chuvilin
IFK FSU
Jena
Germany



From daemon Fri Apr 27 05:24:29 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 27 Apr 2001 12:20:36 +0200
Subject: Meeting Anouncement: EuroFE 2001

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Dear All,

EuroFE 2001, the European Meeting on Applications of Field Emission
Technologies, EuroFE 2001 will tale palace this year in University of
Alicante, Spain from November 12-16, 2001. Details are available at
www.cmp-cientifica.com/eurofe2001

The fundamental purpose of workshops such as EUROFE2001 consists in bringing
together a hundred scientific, leaders in this area, in order to:
Link all active research groups in Field Emission.

Link all industries interested in Field Emission. At EUROFE2001, major
companies such as Motorola (USA), Candescent (USA), Samsung (Korea), PixTech
(France), Saint-Gobain (France), Thomson (France), LG (Korea) and PFE (UK)
will be present.

Foster co-operation and the interchange of ideas between research groups and
European Industry.

Provide an overview of the research and commercialisation of Field Emission
technologies within Europe. During EUROFE2001, a session will be dedicated
to Space Applications (ESA/ESTEC speakers).

Allow rapid and flexible response to new technological challenges

Based on the format established at EUROFE2000, ample time for discussion
will be available for meaningful interaction between senior
researchers/graduate students and industrial partners.

Funds to offset travel expenses for graduate students to present their work
in a poster session at EUROFE2001 will be available.

For further details please contact:

Antonio Correia
CMP Cientifica
Apdo. Correos 20
28230 Las Rozas (Madrid), Spain
Tel: +34 91 6407187
Fax: +34 91 6407186

Regards,

Tim

*****************************************************************
Tim E. Harper CEO
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/




From daemon Fri Apr 27 05:27:08 2001



From: Yuan Wu :      wy-at-istec.or.jp
Date: 27 Apr 01 19:22:01 -0800
Subject: about simulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, everyone,
Now, I need to simulate HRTEM image of a phase that I can not find its space group in X-ray powder diffraction files. Do you know whether it is possible for me to find its atomic positions. Could you please give me suggestions?
Thank you very much in advance.
WY




From daemon Fri Apr 27 05:32:01 2001



From: Gary Dietrich Chinga :      garyc-at-stud.ntnu.no
Date: Fri, 27 Apr 2001 12:27:17 +0200 (MET DST)
Subject: Help...

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Hi!

I am wondering why I do not get any more mails from the list since
December last year. Is the microscopy list still active.

Thanks.

Gary.



From daemon Fri Apr 27 07:28:57 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 4/26/01 2:27 PM
Subject: carbon black in polymer

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Carbon black in polymer or rubber will cause no more problems than
most any other hydrocarbon. The electron beam does sublimate the
plastic. Especially when working with pure carbon black, one can
essentially watch a contaminant layer grow on the constituent
particles of a given aggregate. A cold finger is a very good idea but
not absolutely required if you are just scanning the sample.

Our Philips 300 is still in excellent operating condition after 28
years of carbon black work.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX


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Does carbon black cause a problem in the TEM? What percautions should I
take? Is the use of the cold finger recommended?





From daemon Fri Apr 27 07:38:44 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 27 Apr 2001 13:33:59 +0100 (GMT Daylight Time)
Subject: Re: re Undergraduate EM courses

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Back in 1989 all Biology degree and sub-degree students
attended 3 sessions in the EM Unit. Now the expansion in
numbers precludes such demonstrations. I regret this
change.

In the first year they get a few lectures and a "dry" class
identifying cell organelles. In the final year about 6
students do major projects in the lab.

We still do undergraduate classes in dust ID and in EDX for
modules taken by Chemistry, Environmental Science and
Environmental Health students as the numbers are smaller.

Dave


On Fri, 27 Apr 2001 16:08:08 +1000 Dr Deborah Stenzel
{d.stenzel-at-qut.edu.au} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear Robert and all
}
} I have the pleasure of teaching full semester (13 week) EM course to
} undergraduates at Queensland University of Technology in Brisbane,
} Australia. We've offered this course for quite a number of years - in
} fact, even had it extended from part of a broader topic to a full semester
} just for EM! The course is offered as part of the School of Life Sciences
} undergraduate program and, hence, primarily addresses biological
} EM. However, it is open to students from other disciplines - presently we
} do not have a full EM course in the physical sciences, although my
} colleague Dr Thor Bostrom teaches several weeks of EM in various courses
} for physics, chemistry and engineering undergraduate students.
}
} Apart from this, I will usually run single 2 hour practical sessions for a
} couple of other undergraduate courses (mainly microbiology, where students
} look at negatively stained microbes they have isolated from various
} sources). I'll often be asked to give a half hour (sometimes an hour)
} introductory EM lecture to these students, prior to the practicals.
}
} I also teach a brief introduction (2-3 weeks) to EM as part of a
} post-graduate course on cell structure and function in pathology. Other
} post-graduates needing EM as part of their research are taught individually
} by staff in our EM Facility, to the level that their projects require.
}
}
} My full semester EM course is offered in the third year of the B. Applied
} Science course. This year I have 30 students - the numbers have been
} increasing over the past few years from a previous average of 20. I
} usually also have a couple of post-graduate (research) students attending
} the lectures - these students will be using our EM facilities as part of
} their research, and I find this is an effective means of giving them some
} useful background and theory to support their laboratory work.
}
} For this course, I am allocated two by one hour lectures each week (total
} of 26 hours lectures for the semester). I cover basic instrumentation
} (TEM, SEM, preparation equipment) and biological sample preparation
} methods, including cryotechniques. I also include immunolabelling methods,
} some cytochemistry, microanalysis (Thor Bostrom gives these lectures), a
} few interesting "non-biological" applications, and a brief introduction to
} some other imaging methods (confocal, AFM etc).
}
} Practical sessions have been a bit of a "challenge" as student numbers have
} increased and EM staff numbers have decreased! However, I've now got a
} system that works, and doesn't cause me (or the equipment) too much
} stress. I have two by 3 hours practical sessions running each week - ie. 6
} hours of my teaching time per week (total of 72 hours of my teaching time
} for the semester).
}
} Each student attends a total of 24 hours practical sessions for the
} semester (this fits with our "official" allocation for total practical time
} for a course of this credit point value). I divide the class into small
} groups - 3 to 4 students per group, with current student numbers. For
} preparation lab based pracs (where we have enough space and the students
} don't need such close supervision), I'll have a number of these small
} groups attending the same practical session. For EM operation and viewing
} pracs, I'll only have one small group attending at a time (usually 1 or 1.5
} hours of the session for each small group). This has enabled me to run
} practical sessions almost single-handed - money to employ
} tutors/demonstrators or other teaching assistants has been a bit difficult
} to obtain! If anyone would like further information on the practicals I
} run or on the logistics of this, please contact me by direct email - does
} anyone else out there run EM practicals for undergraduates??? I haven't
} seen a rush of emails in response to Robert's questions.
}
} Fortunately, my university is still keen to have students doing "hands-on"
} practicals in their courses. However, I've certainly had to decrease
} practical work substantially since I first started teaching in this EM
} course. A lot of this is due to decreased staff numbers in our EM Facility
} - a problem that I guess most of us face. I teach other courses
} (microbiology, parasitology) and encounter the same problems there - it is
} now very difficult to get competent, experienced people to assist in
} practical classes, particularly for more advanced or specialised courses.
}
} On the bright side, I've found the students enjoy EM a great deal - and it
} is one of the few opportunities our undergraduates get to use expensive and
} (relatively!) modern instrumentation, even if it is only on a very limited
} scale. I've not had many students who don't show a great deal of
} enthusiasm when told it is now their turn to operate the TEM or SEM and
} take some micrographs. This semester, more so than previously, at the end
} of each practical session quite a number of my students have taken the time
} to thank me for running the practicals (even after the night classes which
} finish at 8pm). I figure that has got to be a good sign!!!
}
}
} Regards
} Deb
} *****************************************************
} Dr Deborah Stenzel
} Lecturer (Microbiology)
} School of Life Sciences
} and
} Applications Specialist (Biological)
} Analytical Electron Microscopy Facility
} Queensland University of Technology
} GPO Box 2434
} Brisbane 4001
} Australia
}
} Phone + 61 7 3864 5036
} Fax + 61 7 3864 5100
} email d.stenzel-at-qut.edu.au
}
} http://www.sci.qut.edu.au/aemf
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Apr 27 08:20:58 2001



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Fri, 27 Apr 2001 09:19:52 -0400
Subject: re Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Deb and others,

An interesting thread...

You make mention of "the EM staff" - I'm wondering how many people are
actually involved in teaching the course components, and how much time do
they collectively spend, for example per student per week??

I'm curious to know because we have two people involved in teaching a full
semester Bio EM course for up to 8 students. The students in the end turn in
a fairly polished portfolio (with about a dozen 8x10 micrographs plus figure
legends, from three different tissues that they have processed, cut, and
scoped).

Questions have recently arisen as to how many man-hours we 'should' allot to
such a course, which is far outside the norm for our other lab courses.

I'm interested in off-line dialogue, if others are.

Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford CT 06106
v. 860-297-4289
e. ann.lehman-at-trincoll.edu

-----Original Message-----
} From: Dr Deborah Stenzel [mailto:d.stenzel-at-qut.edu.au]
Sent: Friday, April 27, 2001 2:08 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Robert and all

I have the pleasure of teaching full semester (13 week) EM course to
undergraduates at Queensland University of Technology in Brisbane,
Australia. We've offered this course for quite a number of years - in
fact, even had it extended from part of a broader topic to a full semester
just for EM! The course is offered as part of the School of Life Sciences
undergraduate program and, hence, primarily addresses biological
EM. However, it is open to students from other disciplines - presently we
do not have a full EM course in the physical sciences, although my
colleague Dr Thor Bostrom teaches several weeks of EM in various courses
for physics, chemistry and engineering undergraduate students.

Apart from this, I will usually run single 2 hour practical sessions for a
couple of other undergraduate courses (mainly microbiology, where students
look at negatively stained microbes they have isolated from various
sources). I'll often be asked to give a half hour (sometimes an hour)
introductory EM lecture to these students, prior to the practicals.

I also teach a brief introduction (2-3 weeks) to EM as part of a
post-graduate course on cell structure and function in pathology. Other
post-graduates needing EM as part of their research are taught individually
by staff in our EM Facility, to the level that their projects require.


My full semester EM course is offered in the third year of the B. Applied
Science course. This year I have 30 students - the numbers have been
increasing over the past few years from a previous average of 20. I
usually also have a couple of post-graduate (research) students attending
the lectures - these students will be using our EM facilities as part of
their research, and I find this is an effective means of giving them some
useful background and theory to support their laboratory work.

For this course, I am allocated two by one hour lectures each week (total
of 26 hours lectures for the semester). I cover basic instrumentation
(TEM, SEM, preparation equipment) and biological sample preparation
methods, including cryotechniques. I also include immunolabelling methods,
some cytochemistry, microanalysis (Thor Bostrom gives these lectures), a
few interesting "non-biological" applications, and a brief introduction to
some other imaging methods (confocal, AFM etc).

Practical sessions have been a bit of a "challenge" as student numbers have
increased and EM staff numbers have decreased! However, I've now got a
system that works, and doesn't cause me (or the equipment) too much
stress. I have two by 3 hours practical sessions running each week - ie. 6
hours of my teaching time per week (total of 72 hours of my teaching time
for the semester).

Each student attends a total of 24 hours practical sessions for the
semester (this fits with our "official" allocation for total practical time
for a course of this credit point value). I divide the class into small
groups - 3 to 4 students per group, with current student numbers. For
preparation lab based pracs (where we have enough space and the students
don't need such close supervision), I'll have a number of these small
groups attending the same practical session. For EM operation and viewing
pracs, I'll only have one small group attending at a time (usually 1 or 1.5
hours of the session for each small group). This has enabled me to run
practical sessions almost single-handed - money to employ
tutors/demonstrators or other teaching assistants has been a bit difficult
to obtain! If anyone would like further information on the practicals I
run or on the logistics of this, please contact me by direct email - does
anyone else out there run EM practicals for undergraduates??? I haven't
seen a rush of emails in response to Robert's questions.

Fortunately, my university is still keen to have students doing "hands-on"
practicals in their courses. However, I've certainly had to decrease
practical work substantially since I first started teaching in this EM
course. A lot of this is due to decreased staff numbers in our EM Facility
- a problem that I guess most of us face. I teach other courses
(microbiology, parasitology) and encounter the same problems there - it is
now very difficult to get competent, experienced people to assist in
practical classes, particularly for more advanced or specialised courses.

On the bright side, I've found the students enjoy EM a great deal - and it
is one of the few opportunities our undergraduates get to use expensive and
(relatively!) modern instrumentation, even if it is only on a very limited
scale. I've not had many students who don't show a great deal of
enthusiasm when told it is now their turn to operate the TEM or SEM and
take some micrographs. This semester, more so than previously, at the end
of each practical session quite a number of my students have taken the time
to thank me for running the practicals (even after the night classes which
finish at 8pm). I figure that has got to be a good sign!!!


Regards
Deb
*****************************************************
Dr Deborah Stenzel
Lecturer (Microbiology)
School of Life Sciences
and
Applications Specialist (Biological)
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

Phone + 61 7 3864 5036
Fax + 61 7 3864 5100
email d.stenzel-at-qut.edu.au

http://www.sci.qut.edu.au/aemf



From daemon Fri Apr 27 08:40:02 2001



From: Gary Radice :      gradice-at-richmond.edu
Date: Fri, 27 Apr 2001 09:29:57 -0400
Subject: undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Like Chris, our department does not teach techniques courses.
However, I include SEM and TEM in a course called Microanatomy (about
16 students every fall semester). What I have done is combine
more-or-less traditional histology class lectures with a lab
component in which students compare the histology of the same organ
from a mouse and frog. They make their own paraffin, methacrylate,
and epoxy plastic sections and critical point dry their other
samples. I spend a great deal of time on light microscopy theory, how
to clean and align a light microscope, capture digital images, and
prepare micrographs for illustration (scale bars and labels). I do
not spend a lot of time on the use of the electron microscopes apart
from showing students enough for them to change mag, focus, and take
a picture. Since I can't do everything, I've decided that more
students will find basic light microscopy more generally useful in
the future than EM techniques. However, the students really love
using the "big iron" and are especially enthusiastic about SEM.

Cutting thin sections with glass knives has also been a bottleneck
for my students so I was glad to see the discussion on this recently.
Thanks, Dick, for summarizing the responses.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice


From daemon Fri Apr 27 08:43:14 2001



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 27 Apr 2001 09:27:55 -0400
Subject: TEM negative holders for the Polaroid SprintScan 45 Ultra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

We took one of the smaller negative holders for which we didn't foresee a
need and submitted it along with a TEM negative to a machinist in our shop.
He was able to mill the opening such that it was the ideal size for the 3.25
x 4.25 negative. To give it that professional look he completed the job by
touching up the milled edges with black paint.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


-----Original Message-----
} From: Paula Allan-Wojtas [mailto:AllanWojtasP-at-em.agr.ca]
Sent: Thursday, April 26, 2001 12:17 PM
To: microscopy-at-sparc5.microscopy.com


Hi, all,

Following the recent discussion thread of scanners, I wanted to say that we
are the proud owners of a Polaroid SprintScan 45 Ultra, and are very happy
with the results we have gotten from it. It came with a number of negative
holders covering a range of sizes from 35mm to 4x5, except one for 3.25x4.25
TEM sheet film. Right now we're trying to think of ways around this problem
and would be happy to hear offline from anyone who has some ideas and/or
solutions. I would even entertain the idea of having a holder made (this is
not an option locally for me).

Thanks in advance for any help (and there is usually plenty, thanks to this
list!).

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Fri Apr 27 11:15:41 2001



From: Bruce Brinson :      brinson-at-rice.edu
Date: Fri, 27 Apr 2001 11:15:01 -0700
Subject: Re: carbon black in polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I feel that I must add to this because as a general statement I believe it is
mis-leading ... with respect to carbon black & many other carbon species... some
7000 images of carbon later I suggest that watching carbon morph in the beam is an
exception not the rule. If you sit on anything long enough it will collect carbon
but let's remain objective. Among other things, I have looked at CBs right out of
reactors & extracted from polymers.
How clean your instrument is can be a significant issue. My tool is a JEOL 2010,
LaB6 operating at ~2x10-5Pa, & I use an LN2 cooled anti contamination device, ACD in
Jeol lingo. The stage is room temperature and I usually work at 100 kV for the
contrast enhancement.
I have witnessed such things in experimental materials that I will term "highly
reactive carbons". This lore of morphing carbon may be the most commonly used
challenge of conclusions drawn from TEM data. Stories of exceptions tend to
propagate over space & time. Don't get me wrong, it does happen.

Diverging a bit...

Of much more concern to anyone working in carbon is the carbon contamination
supplied on new, right out of the box carbon coated grid formvar &/or Lacey carbon
grid. The is a real problem for the manufactures & to my knowledge, despite any
claims you may hear, no one has solved this problem.
Once you understand & know the many habits carbon can have, that is the stuff
supplied on new grids, you will realize that many images you see in the literature
could easily be store bought carbon grid contamination. I have personally recorded
dozens of such images... onions, fibers, rods, angular faceted bits, plates,
turbostratic plates, polycrystalline graphite, CB looking stuff, graphitic ribbons &
oh the nano structures, horns, fibers, .... Oh yea, watch out for very thin, low
contrast crystals that throw a textbook perfect [001] hexagonal DP. it is not
carbon. I don't know what it is. Weather on not you have realized it, you have seen
them in imaging mode & probably passed them off as film thickness irregularities.
I am looking at an image computer directory titled "grid stuff". it contains 12
images recorded on one new out of the box lacey carbon grid. Their titles are: hex
plate, nest-2, nest -1, plates, rapping ribbon, plates plus, ribbon, fiber, rodlike,
nanohorn, elongated hex.
To all of this, let me refer you to an article recently or soon to be published
in Carbon. I believe the author's name is Harris. I apologize for the poor quality
reference, I've spaced out my copy of the preprint. It pretty much reads as
something I have contemplated writing over the years but...

it's real folks.


Bruce Brinson
Rice U.

Guess there is a reason ASTM standards require 1000 representative images.


Chuck Butterick wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Carbon black in polymer or rubber will cause no more problems than
} most any other hydrocarbon. The electron beam does sublimate the
} plastic. Especially when working with pure carbon black, one can
} essentially watch a contaminant layer grow on the constituent
} particles of a given aggregate. A cold finger is a very good idea but
} not absolutely required if you are just scanning the sample.
}
} Our Philips 300 is still in excellent operating condition after 28
} years of carbon black work.
}
} Chuck Butterick
} Engineered Carbons, Inc.
} Borger, TX
}
} ______________________________ Reply Separator _________________________________
} Subject: carbon black in polymer
} Author: Margaret Miller {MILLERMM-at-uthscsa.edu} at INTERNET-MAIL
} Date: 4/26/01 2:27 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does carbon black cause a problem in the TEM? What percautions should I
} take? Is the use of the cold finger recommended?
}
}



From daemon Fri Apr 27 12:21:52 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Fri, 27 Apr 2001 13:17:07 -0400
Subject: TEM negative holders for the Polaroid SprintScan 45 Ultra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just turn the So-163 film sideways on the 4x5 holder.

Paul
PPG Industries

-----Original Message-----
} From: Gerroir, Paul J [mailto:Paul.Gerroir-at-crt.xerox.com]
Sent: Friday, April 27, 2001 6:28 AM
To: Paula Allan-Wojtas; microscopy-at-sparc5.microscopy.com


Paula,

We took one of the smaller negative holders for which we didn't foresee a
need and submitted it along with a TEM negative to a machinist in our shop.
He was able to mill the opening such that it was the ideal size for the 3.25
x 4.25 negative. To give it that professional look he completed the job by
touching up the milled edges with black paint.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


-----Original Message-----
} From: Paula Allan-Wojtas [mailto:AllanWojtasP-at-em.agr.ca]
Sent: Thursday, April 26, 2001 12:17 PM
To: microscopy-at-sparc5.microscopy.com


Hi, all,

Following the recent discussion thread of scanners, I wanted to say that we
are the proud owners of a Polaroid SprintScan 45 Ultra, and are very happy
with the results we have gotten from it. It came with a number of negative
holders covering a range of sizes from 35mm to 4x5, except one for 3.25x4.25
TEM sheet film. Right now we're trying to think of ways around this problem
and would be happy to hear offline from anyone who has some ideas and/or
solutions. I would even entertain the idea of having a holder made (this is
not an option locally for me).

Thanks in advance for any help (and there is usually plenty, thanks to this
list!).

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Fri Apr 27 13:08:38 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 27 Apr 2001 13:13:22 -0500
Subject: Re: Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Robert & everyone else,

I used to teach an EM course to the graduate students here. It would
run for 10 weeks and would consist of a 2-2.5 hour lecture period
followed by a lunch break and then a 4 hour lab session during which
I would teach them the "technique du jour" (fixation, embedding,
block trimming, thick sectioning, thin sectioning, SEM sample prep,
darkroom techniques, use of the EM....and in my really crazy days,
alignment of the column!). the class would then be expected to come
in during the week to put into practice what we had done in class.
by the end of the class I expected each to have produces block,
slides with stained thicks, grids with thins and 8X10 printed
micrographs that showed me more than highly enlarged organelles.

I was also supposed to be running the dept's EM facility through all
this. I would tell all of my usual clients not to expect anything
substantial from me while the course ran, because I was too busy
holding the hands of my 5-10 students. It would amount to 20-30
contact hours/week for the 10 week run. Exhausting.

Now that my facility is a college core, I can't just "drop out" for
10 weeks, and so the course is no longer offered.

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Apr 27 16:25:31 2001



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Fri, 27 Apr 2001 09:19:52 -0400
Subject: re Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Deb and others,

An interesting thread...

You make mention of "the EM staff" - I'm wondering how many people are
actually involved in teaching the course components, and how much time do
they collectively spend, for example per student per week??

I'm curious to know because we have two people involved in teaching a full
semester Bio EM course for up to 8 students. The students in the end turn in
a fairly polished portfolio (with about a dozen 8x10 micrographs plus figure
legends, from three different tissues that they have processed, cut, and
scoped).

Questions have recently arisen as to how many man-hours we 'should' allot to
such a course, which is far outside the norm for our other lab courses.

I'm interested in off-line dialogue, if others are.

Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford CT 06106
v. 860-297-4289
e. ann.lehman-at-trincoll.edu

-----Original Message-----
} From: Dr Deborah Stenzel [mailto:d.stenzel-at-qut.edu.au]
Sent: Friday, April 27, 2001 2:08 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Robert and all

I have the pleasure of teaching full semester (13 week) EM course to
undergraduates at Queensland University of Technology in Brisbane,
Australia. We've offered this course for quite a number of years - in
fact, even had it extended from part of a broader topic to a full semester
just for EM! The course is offered as part of the School of Life Sciences
undergraduate program and, hence, primarily addresses biological
EM. However, it is open to students from other disciplines - presently we
do not have a full EM course in the physical sciences, although my
colleague Dr Thor Bostrom teaches several weeks of EM in various courses
for physics, chemistry and engineering undergraduate students.

Apart from this, I will usually run single 2 hour practical sessions for a
couple of other undergraduate courses (mainly microbiology, where students
look at negatively stained microbes they have isolated from various
sources). I'll often be asked to give a half hour (sometimes an hour)
introductory EM lecture to these students, prior to the practicals.

I also teach a brief introduction (2-3 weeks) to EM as part of a
post-graduate course on cell structure and function in pathology. Other
post-graduates needing EM as part of their research are taught individually
by staff in our EM Facility, to the level that their projects require.


My full semester EM course is offered in the third year of the B. Applied
Science course. This year I have 30 students - the numbers have been
increasing over the past few years from a previous average of 20. I
usually also have a couple of post-graduate (research) students attending
the lectures - these students will be using our EM facilities as part of
their research, and I find this is an effective means of giving them some
useful background and theory to support their laboratory work.

For this course, I am allocated two by one hour lectures each week (total
of 26 hours lectures for the semester). I cover basic instrumentation
(TEM, SEM, preparation equipment) and biological sample preparation
methods, including cryotechniques. I also include immunolabelling methods,
some cytochemistry, microanalysis (Thor Bostrom gives these lectures), a
few interesting "non-biological" applications, and a brief introduction to
some other imaging methods (confocal, AFM etc).

Practical sessions have been a bit of a "challenge" as student numbers have
increased and EM staff numbers have decreased! However, I've now got a
system that works, and doesn't cause me (or the equipment) too much
stress. I have two by 3 hours practical sessions running each week - ie. 6
hours of my teaching time per week (total of 72 hours of my teaching time
for the semester).

Each student attends a total of 24 hours practical sessions for the
semester (this fits with our "official" allocation for total practical time
for a course of this credit point value). I divide the class into small
groups - 3 to 4 students per group, with current student numbers. For
preparation lab based pracs (where we have enough space and the students
don't need such close supervision), I'll have a number of these small
groups attending the same practical session. For EM operation and viewing
pracs, I'll only have one small group attending at a time (usually 1 or 1.5
hours of the session for each small group). This has enabled me to run
practical sessions almost single-handed - money to employ
tutors/demonstrators or other teaching assistants has been a bit difficult
to obtain! If anyone would like further information on the practicals I
run or on the logistics of this, please contact me by direct email - does
anyone else out there run EM practicals for undergraduates??? I haven't
seen a rush of emails in response to Robert's questions.

Fortunately, my university is still keen to have students doing "hands-on"
practicals in their courses. However, I've certainly had to decrease
practical work substantially since I first started teaching in this EM
course. A lot of this is due to decreased staff numbers in our EM Facility
- a problem that I guess most of us face. I teach other courses
(microbiology, parasitology) and encounter the same problems there - it is
now very difficult to get competent, experienced people to assist in
practical classes, particularly for more advanced or specialised courses.

On the bright side, I've found the students enjoy EM a great deal - and it
is one of the few opportunities our undergraduates get to use expensive and
(relatively!) modern instrumentation, even if it is only on a very limited
scale. I've not had many students who don't show a great deal of
enthusiasm when told it is now their turn to operate the TEM or SEM and
take some micrographs. This semester, more so than previously, at the end
of each practical session quite a number of my students have taken the time
to thank me for running the practicals (even after the night classes which
finish at 8pm). I figure that has got to be a good sign!!!


Regards
Deb
*****************************************************
Dr Deborah Stenzel
Lecturer (Microbiology)
School of Life Sciences
and
Applications Specialist (Biological)
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

Phone + 61 7 3864 5036
Fax + 61 7 3864 5100
email d.stenzel-at-qut.edu.au

http://www.sci.qut.edu.au/aemf



From daemon Fri Apr 27 17:25:11 2001



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 27 Apr 2001 17:23:28 -0500
Subject: RE: Bx Turn Around Time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are a diagnostic EM lab in surgical pathology, using wet photography
processes. If we receive a sample early in the morning, we will have
labeled prints in the pathologists hands before 4:30pm the same day. It is
basically 8 working hours for most rush cases. If we get the sample later in
the day, we will finish the next morning, and prefer to polymerize overnight
at 70 deg. C for epon araldite.

In order to speed things up, we put plastic in over at 100 deg C for 1 hr 15
min. The plastic isn't as good, but it does the job. We cure it for 5
minutes.


From daemon Sat Apr 28 11:25:15 2001



From: zaluzec-at-microscopy.com
Date: Sat, 28 Apr 2001 11:19:27 -0500
Subject: Administrivia: Nestor is debugging... Just delete this message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

I'm trying to identify the source of a bad addresses in the data base. Please
just trash this message.

Nestor


From daemon Sat Apr 28 19:02:08 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Sun, 29 Apr 2001 11:18:22 -0500
Subject: Preservaion of colagen and retina

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We offer two EM courses here, one SEM and one TEM. Each is an elective
course taken by a mixture of seniors and graduate students, which runs once
a year for a full semester. I teach the TEM course, and enrollment is
generally anywhere from six to ten students. (More than ten would be a
problem in lab.) We have two 50-minute class periods a week, to give the
students some foundation in relation to the instrument and electron optics,
specimen prep., electron diffraction (we're a materials department), and
imaging. There is also a two-and-a-half hour lab session each week, which
covers basic operation of the microscope, an introduction to specimen
preparation, and indexing of diffraction patterns.

Gill

Dr. Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801
(505) 835-5653
gbond-at-nmt.edu

----- Original Message -----
} From: "Robert Fitton" {fittonro-at-luther.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, April 26, 2001 2:40 PM


Hello fellow microscopists:

Does anyone know good protocols for-

1. retina

2. colagen (to prevent shrinkage in skin tissue)

Thanks again,
Tim Quinn
University of Kansas
Museum of Natural History


From daemon Mon Apr 30 03:56:56 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Mon, 30 Apr 2001 04:41:29 -0400
Subject: TEM, SEM: educational software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

We have a range of interactive EM courses set out in e-book form. We cover
basic SEM, TEM and EDX with more advanced courses in SEM. The pictorial
data are in the form of bitmaps and we allow purchasers to use these in
their own presentations if they wish.

Full details are on our web site

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Mon Apr 30 07:36:59 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Mon, 30 Apr 2001 08:31:05 -0400
Subject: carbon black in polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret,
I take it from your 'subject' you have a solid block of polymer with a CB
dispersion. If this is correct you will have no problem looking at a thin
section. Contrast will be low but a low KV ~60 would be good provided you
keep the sections thin. For better contrast you can pyrolyze the polymer to
improve the contrast. If you would like to do this let me know and I fill
you in on the details.
Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com


-----Original Message-----
} From: Margaret Miller [mailto:MILLERMM-at-uthscsa.edu]
Sent: Thursday, April 26, 2001 3:28:PM
To: MSA


Does carbon black cause a problem in the TEM? What percautions should I
take? Is the use of the cold finger recommended?


From daemon Mon Apr 30 08:09:25 2001



From: Romina Belli :      belli-at-science.unitn.it
Date: Mon, 30 Apr 2001 15:04:20 +0200
Subject: SEM-EDS: change or repair?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I have a problem with my EDS system. I bought it in 1992 with a SEM. Now,
the resolution and the quantification results are getting worse.
Maybe it should be enough changing the window and the detector and testing
all. I'd like to have more than one offering about repairing manufactory
but I don't know to ask about it . Could anyone help me?
Thank's in advance.

Romina Belli


From daemon Mon Apr 30 09:04:45 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Mon, 30 Apr 2001 08:58:59 -0500
Subject: RE: SEM-EDS: change or repair?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear List,

I also am in a very similar situation, with a detector of about the same
age, which appears to have slowly but steadily lost resolution (we're up to
nearly 200 eV full width at half max on the Mn K-alpha line).

I have asked the manufacturer how and why this kind of degradation occurs,
but haven't gotten a clear answer. I realize there are multiple components
of the system from which the problem could be coming (it manifests itself as
an approximately constant 50 eV additional fwhm component on all peaks in
the spectrum regardless of at which energy). However, I'd like to know if
there are reasons why this might be unavoidable in a detector of this age?
If not, what are possible causes and how can one prevent it from happening?


Thanks,
Wharton

} -----Original Message-----
} From: Romina Belli [SMTP:belli-at-science.unitn.it]
} Sent: Monday, April 30, 2001 8:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM-EDS: change or repair?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
} I have a problem with my EDS system. I bought it in 1992 with a SEM. Now,
} the resolution and the quantification results are getting worse.
} Maybe it should be enough changing the window and the detector and testing
} all. I'd like to have more than one offering about repairing manufactory
} but I don't know to ask about it . Could anyone help me?
} Thank's in advance.
}
} Romina Belli


From daemon Mon Apr 30 09:41:33 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Mon, 30 Apr 2001 09:22:19 -0500
Subject: Retina and collagen protocols

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists:

Does anyone have a good protocol for retina fixation for TEM?

I'm dealing with specimens collected in the field and it appears that the
initial field fix- "cacodylate and glut" did not preserve the "rods and
cones".

A good recomendation for a field fix would be appreciated. Would Karnovsky's
work?

I also need to fix frog skin tissue, causing minimal shrinkage. Any
suggestions?

Thanks

Tim Quinn


From daemon Mon Apr 30 11:00:46 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Mon, 30 Apr 2001 11:55:29 -0400
Subject: Re: Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I teach three EM classes here which are undergrad and grad level: EM
Theory (2 credits Spring Semester), TEM Lab (3 credits Spring semester),
and SEM Lab (2 credits fall semester).

The EM Theory class meets 2x 50 mins each week and is lecture only (I
bring in lots of hands on show and tells). It covers SEM, TEM, LM,
photography (Silver & digital), sample prep, and AEM. I have a number of
students who take this without taking the lab courses. The Lab courses
require the EM Theory class.

The both labs meet as a group (class limit of 8-9 students) for 2-5 hours on
Mondays (Scheduled for 3 hours but fixation days run long). The students
then meet one-on-one with a TA for 2hrs on the scope each week. The
students “drive” and the TA’s train/assist/watch. Both Labs cover full
microscope operation (including alignment, operating parameters, imaging
modes, and photography), sample prep (from wet to scope, including
embedment, ultramicrotomy, CPD, particulates, staining techniques - 3
separate preps for TEM and 5 for SEM), darkroom, and digital publication
plate production. Students take a written exam, an oral exam on the scope
(once they “pass” the scope exam they can use the scope without
supervision), and turn in a pretty polished portfolio of images. TEM students
spend significant amounts of additional time sectioning, staining, and
developing film and contact printing.

Yes, these labs are labor intensive (teaching and students), but by the end of
either course the goal is that they are qualified TEM or SEM users. Most of
the students continue on utilizing the scope for research (most undergrads
taking the actually have formal research projects with faculty members that
they continue for 1-2 years plus summers, or on to a masters degree).
Students are accepted into the lab classes based on research needs.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Mon Apr 30 11:13:47 2001



From: Joyce Craig :      j-craig-at-csu.edu
Date: Mon, 30 Apr 2001 11:06:40 -0500
Subject: Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At Chicago State University I teach a 4 credit hour course in TEM in the
fall each year. The students have 2 one-hour lectures per week and 10
hours of supervised lab experience per week. I have usually have 4 to 8
students each year. Since students are scheduled into the lab 2 at a
time, this means that someone is with students 30-50 hours per week.
For the last few years I have had a technologist assistant who has been
able to work with the students, so that we have been able to continue
with research and other work during the semester. It really does take
two people for this sort of class.
In the spring I have taught a 3-credit hour class in SEM. This one has
2 hours of lecture and 6 hours of supervised lab per week.
When my students are done with the class, they can prepare specimens
competently, use the TEM or SEM to take pictures, so some alignment,
understand the principles, and present a simple research project to the
department. In the past that was done as a poster or with slides, but
in the last few years they have been preparing Power Point
presentations.



From daemon Mon Apr 30 12:04:20 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Mon, 30 Apr 2001 12:59:11 -0400
Subject: Re: Undergraduate EM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I teach three EM classes here which are undergrad and grad level: EM
Theory (2 credits Spring Semester), TEM Lab (3 credits Spring semester),
and SEM Lab (2 credits fall semester).

The EM Theory class meets 2x 50 mins each week and is lecture only (I
bring in lots of hands on show and tells). It covers SEM, TEM, LM,
photography (Silver & digital), sample prep, and AEM. I have a number of
students who take this without taking the lab courses. The Lab courses
require the EM Theory class.

The both labs meet as a group (class limit of 8-9 students) for 2-5 hours on
Mondays (Scheduled for 3 hours but fixation days run long). The students
then meet one-on-one with a TA for 2hrs on the scope each week. The
students “drive” and the TA’s train/assist/watch. Both Labs cover full
microscope operation (including alignment, operating parameters, imaging
modes, and photography), sample prep (from wet to scope, including
embedment, ultramicrotomy, CPD, particulates, staining techniques - 3
separate preps for TEM and 5 for SEM), darkroom, and digital publication
plate production. Students take a written exam, an oral exam on the scope
(once they “pass” the scope exam they can use the scope without
supervision), and turn in a pretty polished portfolio of images. TEM students
spend significant amounts of additional time sectioning, staining, and
developing film and contact printing.

Yes, these labs are labor intensive (teaching and students), but by the end of
either course the goal is that they are qualified TEM or SEM users. Most of
the students continue on utilizing the scope for research (most undergrads
taking the actually have formal research projects with faculty members that
they continue for 1-2 years plus summers, or on to a masters degree).
Students are accepted into the lab classes based on research needs.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Mon Apr 30 12:05:37 2001



From: W. Michael Schoel :      schoel-at-ucalgary.ca
Date: Mon, 30 Apr 2001 10:56:22 -0600
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






ELECTRON MICROSCOPY TECHNICIAN

The Microscopy and Imaging Facility of the University of Calgary
requires an Electron Microscopy Technician to fill a newly
created position within the unit.

The Microscopy and Imaging Facility is a service resource for the
University of Calgary supporting users campus wide. The
unit works in all areas of science including medicine, biology,
chemistry, materials engineering, and geology. The unit is
equipped with six electron beam instruments, X-ray micro analyzers,
confocal microscope, digital imaging / image analysis
and support resources. It is the largest unit of its type in Western
Canada.

The successful applicant will have work experience in the operation and
routine maintenance of transmission electron
microscopes. The demonstrated ability to teach and instruct new users on
instrumentation will be a definite asset.
Competence in the operation and use of computers and common software
packages is a must.

Salary for this position will be dependant on education and experience
in the field.

Please send by May 14, 2001 your cv and a covering letter that includes
details of your experience that is relevant to this
position to:
Dr. John Reynolds
Department of Cell Biology and Anatomy
University of Calgary,
3330 Hospital Dr NW, T2N 1N4, Calgary, Alberta, Canada.



From daemon Mon Apr 30 12:07:24 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 30 Apr 2001 13:04:00 -0400
Subject: RE: SEM-EDS: change or repair?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







I also am in a very similar situation, with a detector of about the same
age, which appears to have slowly but steadily lost resolution (we're up to
nearly 200 eV full width at half max on the Mn K-alpha line).

I have asked the manufacturer how and why this kind of degradation occurs,
but haven't gotten a clear answer. I realize there are multiple components
of the system from which the problem could be coming (it manifests itself as
an approximately constant 50 eV additional fwhm component on all peaks in
the spectrum regardless of at which energy). However, I'd like to know if
there are reasons why this might be unavoidable in a detector of this age?
If not, what are possible causes and how can one prevent it from happening?

Wharton

} Hi!
} I have a problem with my EDS system. I bought it in 1992 with a SEM. Now,
} the resolution and the quantification results are getting worse.
} Maybe it should be enough changing the window and the detector and testing
} all. I'd like to have more than one offering about repairing manufactory
} but I don't know to ask about it . Could anyone help me?
} Thank's in advance.
}
} Romina Belli

Dear Wharton and Romina,
Our Kevex detector was installed in the early 80s, and every so often we
have had to warm up and pump out the detector to get back the specified
resolution (145 eV). Last year, when this didn't work, we sent the detector to
Doug Connors (tnas1-at-msn.com), and he cleaned and overhauled it for a good price
and got 147 eV resolution. I have no connection to Doug other than as a
satisfied customer.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Mon Apr 30 12:58:31 2001



From: Katharine Dovidenko :      KDovidenko-at-uamail.albany.edu
Date: Mon, 30 Apr 2001 13:57:05 -0400
Subject: FIB: Au deposition instead of Pt.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all:

This is a question about the FEI FIB 200: We are interested in converting
our Pt Gas Injection System into Au, but have been told about possible
negative effects on the instrument.

Any data/experience/thoughts on Au deposition using FIB (any model) and its
effect on the instrument performance will be greatly appreciated!

Thanks!
P.S. I realize it is not quite a microscopy question. I have posted it to
the FIB-users list, but received only one response so far. Apologize to
those who will get this message for the second time.

********************************
Katharine Dovidenko, Ph.D.
Scientist
UAlbany Institute for Materials and Center for Advanced Thin Film Technology
University at Albany
SUNY
www.albany.edu/cat

251 Fuller Rd.
Albany, NY 12203
USA
Phone: (518) 437-8781
Fax: (518) 437-8687



From daemon Mon Apr 30 13:54:22 2001



From: zaluzec-at-microscopy.com
Date: Mon, 30 Apr 2001 13:52:24 -0500
Subject: Administrivia: Listserver shutting down for a day or so

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

I've got to do some system software maintenance & upgrades.

As a result you can expect the server will be off-line for about a day
some time withing the next few days.

Nestor
Your Friendly Neighborhood SysOp


From root Mon Apr 30 14:10:53 2001
Return-Path: {Microscopy-request-at-sparc5.microscopy.com}
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Message-Id: {p05001900b7135f2023bd-at-[206.69.208.21]}


Colleagues

I've got to do some system software maintenance & upgrades.

As a result you can expect the server will be off-line for about a day
some time withing the next few days.

Nestor
Your Friendly Neighborhood SysOp


From daemon Mon Apr 30 15:17:03 2001



From: Goheen, Michael P. :      mgoheen-at-iupui.edu
Date: Mon, 30 Apr 2001 15:12:37 -0500
Subject: FW: SEM position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} -----Original Message-----
} From: Gonzalez-Cabezas, Carlos
} Sent: Monday, April 30, 2001 2:37 PM
} To: Goheen, Michael P.
} Subject: RE: SEM position
}
} I was asked to post this job opening on the listserver.
}
} Mike Goheen
}
} SEM/EPMA tech wanted. The Indiana University School of Dentistry is
} looking for a technician for its new digital electron microscopy
} laboratory.
} A JEOL LV-5310 scanning electron microscope and JEOL 8900R electron probe
} microanalyzer are in the process of being installed in a renovated lab in
} the IU School of Dentistry. The electron microscopes will have energy- and
} wavelength-dispersive spectrometers and are fully digitized. The
} laboratory
} will serve the research and teaching interests of several units on campus
} in
} addition to Dentistry including the IU School of Medicine, and the Purdue
} School of Science (Departments of Geology, Biology, Chemistry, and
} Physics)
} and Purdue School of Engineering at Indianapolis. We seek a candidate who
} has a range of interests in spatial variations of the microstructure and
} composition of materials, and skills in one or more fields such as
} computer
} technology, electron microscopy, materials science, engineering
} technology,
} biomedical and geological research. Please contact Dr. Carlos Gonzalez,
} Preventive Dentistry Department, IU School of Dentistry.
}
} Dr. Carlos González-Cabezas, DDS, PhD
} Director of the Confocal & Scanning Electron Microscopy Facility
} Indiana University School of Dentistry
} CGONZALE-at-IUPUI.EDU
}
}
}
} -----Original Message-----
} From: Goheen, Michael P.
} Sent: Wednesday, April 11, 2001 2:17 PM
} To: Gonzalez-Cabezas, Carlos
} Subject: RE: SEM position
}
}
} -----Original Message-----
} From: Gonzalez-Cabezas, Carlos
} Sent: Wednesday, April 11, 2001 12:20 PM
} To: Goheen, Michael P.
} Subject: SEM position
}
}


From daemon Mon Apr 30 15:54:20 2001



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Mon, 30 Apr 2001 16:02:59 -0500
Subject: FRET/FLIM Symposium-Early Registration Deadline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{bold} {color} {param} ffff,0000,0000 {/param} {bigger} FYI: This is the last
day for early registration for the FRET/FLIM Symposium in San Antonio.=20
Abstracts will be accepted until June 1st.

{/bigger} {/color} {/bold} {bigger} The University of Texas Health Science
Center will host a symposium sponsored by Hamamatsu Photonics KK on

{/bigger}

{bold} {color} {param} 0000,0000,ffff {/param} {bigger} {bigger} {bigger} FRET
and FLIM:

{/bigger} {/bigger} {/bigger} {/color} {/bold} {bold} {bigger} Advanced
Fluorescence Techniques for Biological Imaging

{/bigger} {/bold} {color} {param} 0000,0000,ffff {/param}

{bold} {bigger} {bigger} June 8-10, 2001 {/bigger} {/bigger} {/bold} {/color} =20


{bold} at {/bold} =20


{bold} {bigger} The Sheraton Gunter Hotel

205 E. Houston St.

San Antonio, TX

{/bigger} {/bold}

{bold} {color} {param} 0000,0000,ffff {/param} {bigger} Registration Fees

Student: $175 ($200 after May 1st)

Academic/Corporate: $225 ($250 after May 1st)

{/bigger} {/color} {/bold}


Meeting, lodging and travel information may be found at:


{bold} {color} {param} ffff,0000,ffff {/param} {bigger} {bigger} http://usa.hamamat=
su.com/fretflim

{/bigger} {/bigger} {/color} {/bold}

Talks:

Philippe Bastiaens,=20

{paraindent} {param} left,out {/param} "Spatial resolution of early
signalling processes in cells"=20

{/paraindent} Christoph Biskup,=20

{paraindent} {param} left {/param} "Fluorescence lifetime and energy transfer
measurements in living cells with a confocal laser scanning microscope
and a streak camera"=20

{/paraindent} Robert Clegg,=20

{paraindent} {param} left {/param} "Real-time fluorescence lifetime-resolved
imaging - why we do it, how it's done, and applications for biology and
medicine."=20

{/paraindent} Michael Edidin

{paraindent} {param} left {/param} "Photobleaching FRET microscopy: practice
and theory"

{/paraindent} Enrico Gratton =20

Hans Gerritsen

{paraindent} {param} left {/param} "Fast fluorescence lifetime imaging"=20

{/paraindent} Jesus Gonzalez

{paraindent} {param} left {/param} "FRET Probes and Assays for Drug
Discovery"=20

{/paraindent} Brian Herman

{paraindent} {param} left {/param} "FRETing over the apoptotic cascade"

{/paraindent} Thomas Jovin

{paraindent} {param} left {/param} "Extending the capabilities of FRET and
FLIM for molecular and cellular biology: phFRET (photochromic FRET),
rFLIM (anisotropy FLIM), spectrally-resolved and optical-sectioning
FLIM"

{/paraindent} Karsten K=F6nig

{paraindent} {param} left {/param} "Multiphoton microscopy with submicron
spatial and picosecond temporal resolution"=20

{/paraindent} Wen-hong Li

{paraindent} {param} left {/param} "Towards the development of highly
luminescent lanthanide complexes for FRET and FLIM"=20

{/paraindent} Paloma Mas (substituting for Steve Kay)

{paraindent} {param} left {/param} "Functional interaction of phytocrome B
and cryptochrome 2"=20

{/paraindent} Atsushi Miyawaki

{paraindent} {param} left {/param} "Imaging of cellular functions by
FREting"

{/paraindent} Ammasi Periasamy

{paraindent} {param} left {/param} "Quantitation of Protein Signals in a
Single Living Cell: Wide-field, Confocal, Two-photon and Lifetime FRET
Microscopy"=20

{/paraindent} Alexander Sorkin

{paraindent} {param} left {/param} "Interactions of the EGF receptor with
adapter proteins during endocytosis" =20

{/paraindent} Roger Tsien=20

{paraindent} {param} left {/param} "FRET based readouts of intracellular
messengers and protein interactions"=20

{/paraindent}






From daemon Mon Apr 30 18:05:32 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 30 Apr 2001 15:59:13 -0700
Subject: RE: SEM-EDS: change or repair?

Contents Retrieved from Microscopy Listserver Archives
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Dear Wharton and Romina,
I am running two EDX detectors that are older than Romina's, one 1985 and
one of similar age that I bought used. Both still meet their original spec
of 149 and 146 eV at Mn Ka. When I have had a degradation of resolution, I
turned off the bias, grounded it out with a paper clip on the detector bias
connector and warmed up the detector until all the frost was gone from
inside the dewar. I used warmed air from a hair drier, blown into a hose
down to the bottom, but there other methods that can be used. When the dewar
was completely dry, I refillled with liquid nitrogen, let it cool overnight
and applied bias the next day. The bias should be on at least one hour
before testing the resolution. In one case this cured the resolution, but
degraded the holding time for liquid nitrogen, so I then had the dewar
re-pumped.
I would recommend that step, at least, before buying a new detector. There
are also EDX detector repair companies that will diagnose and repair
detectors for less than the cost of a new one. I have also had a grounding
problem that gave high noise on the detector, because the case on the
pre-amp oxidized. A little emery paper cured that. That showed more high
dead-time than degraded resolution.
At 08:58 AM 4/30/01 -0500, you wrote:
}
} Dear List,
}
} I also am in a very similar situation, with a detector of about the same
} age, which appears to have slowly but steadily lost resolution (we're up to
} nearly 200 eV full width at half max on the Mn K-alpha line).
}
} I have asked the manufacturer how and why this kind of degradation occurs,
} but haven't gotten a clear answer. I realize there are multiple components
} of the system from which the problem could be coming (it manifests itself as
} an approximately constant 50 eV additional fwhm component on all peaks in
} the spectrum regardless of at which energy). However, I'd like to know if
} there are reasons why this might be unavoidable in a detector of this age?
} If not, what are possible causes and how can one prevent it from happening?
}
}
} Thanks,
} Wharton
}
} } -----Original Message-----
} } From: Romina Belli [SMTP:belli-at-science.unitn.it]
} } Sent: Monday, April 30, 2001 8:04 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: SEM-EDS: change or repair?
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi!
} } I have a problem with my EDS system. I bought it in 1992 with a SEM. Now,
} } the resolution and the quantification results are getting worse.
} } Maybe it should be enough changing the window and the detector and testing
} } all. I'd like to have more than one offering about repairing manufactory
} } but I don't know to ask about it . Could anyone help me?
} } Thank's in advance.
} }
} } Romina Belli
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Apr 30 21:15:10 2001



From: David P. Bazett-Jones, Ph.D. :      bazett-at-ucalgary.ca
Date: Mon, 30 Apr 2001 17:57:39 -0600
Subject: Job Posting

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Job Posting submitted by Dr. David P. Bazett-Jones

Service Manager,
Electron Microscopy Facility

Date Posted: April 30, 2001

Position Status: Full-time, Fixed term

Department: Cell Biology
Research Institute

Available: August 1, 2001

Description of the Position: You will share responsibility for the
operation and maintenance of transmission and scanning electron
microscopes in a new Bioimaging Facility co-sponsored by teaching
hospitals in the University of Toronto. The microscopes include an ESEM
(FEI/Philips) and a 200 kV TEM (FEI/Philips) equipped with EDX, GIF and
cryostage. You will also be responsible for coordination and management

of electron bioimaging services required by investigators of the
Hospital for Sick Children Research Institute.

Qualifications: As an ideal candidate, you have completed a M.Sc. in
biological sciences, or have completed a B.Sc. with experience in
analytical electron microscopy, ultramicrotomy and other sample
preparation techniques. Strong computer skills are an asset.

You possess excellent verbal communication and
organizational skills. You have the ability to work well
independently and in a team.

Hours : 35 hours/week

Salary: $39,848.95 - $50,277.67

Available to: Internal and External Candidates

Deadline: May 9, 2001

Submit Resume to : Erin O’Hare
The Hospital for Sick Children
555 University Avenue, Toronto, Ontario
M5G1X8

Fax (416) 813-5671
E-mail: hr.recruiter-at-sickkids.on.ca

Must Quote File Number CG0102-EO

We thank you in advance for your interest. Only those applicants
selected for an interview will be contacted.





From daemon Mon Apr 30 23:43:44 2001



From: zaluzec-at-microscopy.com
Date: Mon, 30 Apr 2001 23:25:31 -0500
Subject: Administrivia: Listserver Back On-Line...

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Colleagues....

Mananged to get most of the OS updated done this evening.
There may be a few hiccups over the next couple of days
so be patient. Be prepared for the occasional error message
while I reset and fine tune all the system parameters

Cheers....
Nestor
Your Friendly Neighborhood SysOp


From daemon Tue May 1 03:08:17 2001



From: charles4627-at-sprintmail.com
Date: Tue, 01 May 2001 03:23:49 -0700
Subject: No More Debt 22865

Contents Retrieved from Microscopy Listserver Archives
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Dear All
Being a botanist and all, I know the cube root of very little about
this really, but my understanding is that gold atoms can diffuse into
and "poison" semiconductors, and that gold should never be used for
specimen coating etc. in any SEM / FIB which may be used to examine or
test semiconductors where the semiconductor functionality must be
maintained. A) Is this relevant to Katharine's question? B) Is it
true or an urban myth?

Chris


----- Original Message -----
} From: "Katharine Dovidenko" {KDovidenko-at-uamail.albany.edu}
To: "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
Sent: Monday, April 30, 2001 6:57 PM






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From daemon Tue May 1 07:23:38 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Tue, 1 May 2001 08:17:47 -0400
Subject: RE: SEM-EDS: change or repair?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wharton, It may be as simple as cleaning out your dewar and or outgassing
your window. We were losing resolution on our EDAX detector. It was brought
up to room temperature for a day or so and the resolution improved greatly.
It worth a try if the manufacturer allows it. Clean out any debris from the
dewar and make sure your detector isn't powered up during the warm up.
Good Luck,
Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com


-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Monday, April 30, 2001 9:59:AM
To: 'Romina Belli'; Microscopy-at-sparc5.microscopy.com



Dear List,

I also am in a very similar situation, with a detector of about the same
age, which appears to have slowly but steadily lost resolution (we're up to
nearly 200 eV full width at half max on the Mn K-alpha line).

I have asked the manufacturer how and why this kind of degradation occurs,
but haven't gotten a clear answer. I realize there are multiple components
of the system from which the problem could be coming (it manifests itself as
an approximately constant 50 eV additional fwhm component on all peaks in
the spectrum regardless of at which energy). However, I'd like to know if
there are reasons why this might be unavoidable in a detector of this age?
If not, what are possible causes and how can one prevent it from happening?


Thanks,
Wharton

} -----Original Message-----
} From: Romina Belli [SMTP:belli-at-science.unitn.it]
} Sent: Monday, April 30, 2001 8:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM-EDS: change or repair?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi!
} I have a problem with my EDS system. I bought it in 1992 with a SEM. Now,
} the resolution and the quantification results are getting worse.
} Maybe it should be enough changing the window and the detector and testing
} all. I'd like to have more than one offering about repairing manufactory
} but I don't know to ask about it . Could anyone help me?
} Thank's in advance.
}
} Romina Belli


From daemon Tue May 1 09:46:24 2001



From: place7-at-mail.com
Date: Wed, 02 May 2001 02:36:59 +1200
Subject: Please reply

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I wish send my Resume to your company. Could you please supply me with the correct persons Name/Department that I should attention it to.

Kind Regards

Julian



From daemon Tue May 1 10:25:12 2001



From: Lou Ross :      masmembership-at-excite.com
Date: Tue, 1 May 2001 08:21:18 -0700 (PDT)
Subject: MAS membership listings

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We are in the process of updating the Microbeam Analysis Society's
membership database. If there are any changes in your personal information
(address, phone/fax #'s, email, etc.) as listed in the 2000 directory and
you have not made the necessary changes on your 2001 renewal form, please
email me with the updated information. Although we will not be printing a
new directory this year we would like to keep everyone as current as
possible with the membership information.

If you are not a member of MAS and would like to join, please contact me for
more information and an application form.

Thanks,
Lou Ross
MAS Membership Services
PMB #141
2101 W. Broadway
Columbia, MO 65203-1261
(800) 4MASMEM
url: www.microanalysis.org





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue May 1 11:35:58 2001



From: JHoffpa464-at-aol.com
Date: Tue, 1 May 2001 12:30:21 EDT
Subject: used ultracut

Contents Retrieved from Microscopy Listserver Archives
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--part1_fc.5bd8704.28203e9d_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

We are currently looking for a used ultrcut microtome in good to excellent
condition.
John Hoffpauir
Cooper hospital
Camden NJ
08107
856 757-7781

--part1_fc.5bd8704.28203e9d_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} We are currently looking for a used ultrcut microtome in good to excellent
{BR} condition.
{BR} John Hoffpauir
{BR} Cooper hospital
{BR} Camden NJ
{BR} 08107
{BR} 856 757-7781 {/FONT} {/HTML}

--part1_fc.5bd8704.28203e9d_boundary--


From daemon Tue May 1 11:45:16 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Tue, 1 May 2001 13:38:05 -0300
Subject: Jeol stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a general question for the List - are those 10mm x 10mm cylindrical
copper (or Al) Jeol type stubs still in wide usage in the SEM community? Do
newer Jeol machines still use them?
The reason I'm asking is that we have a couple cabinets full of these
old stubs from when we used to have a Jeol back in the mid-70's, but of
course can't look at them now with our current instrument. I'd like to toss
them so we can modify the cabinets to accept our pin-type stubs, and I'd
feel better about doing so if I thought it would be hard to find an
instrument that could still look at these old ones. Of course, if it turns
out that I can't find documentation to indicate what's on these stubs,
they'll be going in the bin anyway.

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Tue May 1 11:45:48 2001



From: Brian Matsumoto :      matsumot-at-lifesci.ucsb.edu
Date: Tue, 1 May 2001 09:42:41 -0700
Subject: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
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Workshop Announcement
University of California at Santa Barbara


The Department of Molecular, Cellular, and Developmental Biology and the
Neuroscience Research Institute are sponsoring an advance course on light
microscopy. This 4-day workshop will be offered from August 20 through
August 23, 2001 and will consist of lectures and laboratory exercises that
will run from 9 am to approximately 5 pm each day. The seminar/workshop will
be an intensive lecture/laboratory series that will enable participants to
develop theoretical and hands-on expertise with light microscopes. Attendees
will closely interact with the instructors while using modern research grade
microscopes, cameras, and computers. The seminars and laboratories will
cover basic optical theory and how it pertains to increasing contrast
(signal to noise ratio) in biological samples. Fundamental techniques such
as fluorescence, phase contrast, Nomarski differential interference
contrast, and darkfield imaging will be taught and attendees will use
microscopes equipped to perform these optical enhancement techniques. In
addition, the theory and practice of electronic image acquisition (analog
and digital) will be discussed and attendees will work with low-light
cameras, digital image processing computers, and morphometric programs.
There are five research grade microscopes, five electronic imaging cameras,
two computer workstations, and one confocal microscope. With a maximum
enrollment of 10 students, there will be ample opportunity to work with all
of the microscopes and cameras. For those so interested, intensive hands-on
instruction and guidance on the confocal microscope will be provided.

For a fuller description of the workshop please check the web address
below. Enrollment forms can be completed online and this workshop provides
an opportunity to have a working-vacation in Santa Barbara, California.



http://www.lifesci.ucsb.edu/mcdb/workshop/index.htm





From daemon Tue May 1 12:39:33 2001



From: Smartech :      smartech-at-javanet.com
Date: Tue, 1 May 2001 13:43:32 -0400
Subject: Stand for the Nikon 990

Contents Retrieved from Microscopy Listserver Archives
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The Nikon 990 has a pretty good macro mode and I have found a supplier of a
macro lens system that is excellent, but I need a stand that has rough
height adjustment like you would find on a stereo scope. Has anyone found a
good solution for this presuming that the sample would sit on a table or
stand and the Nikon lens would be lowered to the desired height. I know
this is typically done with a copy stand, but I find them over-kill (large
and provides own illumination). I would use the illumination from my stereo
scope so all I need is a small mechanical stage.

Thanks

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Tue May 1 12:55:31 2001



From: Smartech :      smartech-at-javanet.com
Date: Tue, 1 May 2001 14:01:21 -0400
Subject: I am looking to buy some used LM parts

Contents Retrieved from Microscopy Listserver Archives
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I am looking for the following items:

An apo objective for the M5a and 15X eyepieces (1 or 2) for the M5a.

Also, I am looking for an objective for a Metallurgical LM Unitron MeC3-2313
(plan), 20X, 170 mm.

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Tue May 1 14:47:26 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Tue, 1 May 2001 12:45:48 -0400
Subject: LM: Lens Help, Please (Warning: mundane)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All.

Being an electronics technician, I only know enough about optics
to be dangerous, but use microscopes often. I am trying to
understand what we have, in order to figure out what else could
help us out. I would appreciate information for a "layman", or
pointers web sites that might enlighten me.

First, I need help understanding the markings on our objective
lenses. The question marks indicate what I don't even have
an inkling of the meaning.

#1: Zeiss (The maker of our LSM &
this lens)
Epiplan-NEOFLUAR ( ? )
100x/0,90 (magnification/numerical
aperture)
44 23 80 ( ? )

#2: Olympus
MDPlan 150 ( ? , magnification)
0.95 (numerical aperture)
(infinity sign)/0 f=180 ( ? )

#3 research devices (The maker)
infrared (illumination designed
for)
Trans 100 IRN ( ? ; mag ; near IR ? )
0.90 ( NA )

Is it possible to calculate the working distance from the available
information?

When a lens is made for infrared use, what is different about it?
Are the lens coatings different, or totally absent? Is the lens glass
special?

If you read this far, thank you! I thought I would find the answers to
these questions, and many more, on an episode of "Soap," but
it didn't work. The list is a wonderful pool of knowledge, so don't
fail me now!

Thank you,
Darrell



From daemon Tue May 1 14:47:26 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 01 May 2001 14:43:42 -0500
Subject: Re: Au deposition instead of Pt.

Contents Retrieved from Microscopy Listserver Archives
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Chris: if you MUST maintain functionality, it's best not to coat at all.
My next choice would be
carbon coating, which can be removed by ashing in an O2 plasma. The 3rd
choice is Au/Pd,
which can be removed with a wet etch of iodine/potassium iodide, but this
can attack any
exposed aluminum metal on the die.

The biggest problem with using a coating on semiconductors one wishes to
remain functional is
getting all the coating off to prevent shorts/leakages between the device
pins. I don't think it's
relevant to Katherine's question as she is concerned about negative effects
to her FIB, not the devices.

Most semiconductors have a passivation layer (usually silicon nitride about
1 micron thick) over their
surfaces and this protects from unwanted contaminants. I'm no device
physicist, but I think the
device would have to be heated to a high temperature for any gold implanted
in the top atomic
layers to diffuse into the active junctions and cause trouble. You want to
keep Au out of the fab,
but after the passivation is deposited and is intact, the device is fairly
impervious to metals sputtered
on top. Some older devices used to have Au plated on their backs to help
attach them in their packages.

I've used gold (sputter and evaporative) coating over the years to coat
semiconductors for SEM exam,
and haven't seen any adverse effects. Most of my problems were related to
trying to get the coating
off afterwards.


Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
} Being a botanist and all, I know the cube root of very little about
} this really, but my understanding is that gold atoms can diffuse into
} and "poison" semiconductors, and that gold should never be used for
} specimen coating etc. in any SEM / FIB which may be used to examine or
} test semiconductors where the semiconductor functionality must be
} maintained. A) Is this relevant to Katharine's question? B) Is it
} true or an urban myth?
}
} Chris
}
} ----- Original Message -----
} } From: "Katharine Dovidenko" {KDovidenko-at-uamail.albany.edu}
} To: "'Microscopy-at-MSA.Microscopy.Com'"
} {Microscopy-at-sparc5.microscopy.com}
} Sent: Monday, April 30, 2001 6:57 PM
} Subject: FIB: Au deposition instead of Pt.
}
} } --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
} ---.
} }
} }
} } Dear all:
} }
} } This is a question about the FEI FIB 200: We are interested in
} converting
} } our Pt Gas Injection System into Au, but have been told about
} possible
} } negative effects on the instrument.
} }
} } Any data/experience/thoughts on Au deposition using FIB (any model)
} and its
} } effect on the instrument performance will be greatly appreciated!
} }
} } Thanks!
} } P.S. I realize it is not quite a microscopy question. I have posted
} it to
} } the FIB-users list, but received only one response so far. Apologize
} to
} } those who will get this message for the second time.
} }
} } ********************************
} } Katharine Dovidenko, Ph.D.
} } Scientist
} } UAlbany Institute for Materials and Center for Advanced Thin Film
} Technology
} } University at Albany
} } SUNY
} } www.albany.edu/cat
} }
} } 251 Fuller Rd.
} } Albany, NY 12203
} } USA
} } Phone: (518) 437-8781
} } Fax: (518) 437-8687
} }
} }

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
DSPS Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Tue May 1 16:50:51 2001



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Tue, 01 May 2001 14:44:09 -0700
Subject: Nikon 990 Copystand

Contents Retrieved from Microscopy Listserver Archives
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Hi Ric,

For macro photography I use a machinist's height gauge. They are
usually about 18" high with a smooth, but tight, sliding anvil that can

be machined to accept a camera adapter ring mount. A threaded fine
adjustment allows for fine focus. Mounting a Nikon 990 will require
that additional weight be added to the gauge's base to prevent the
assembly from toppling. Lubricated glass plates and a tiny sandbox can

be used for orienting the sample relative to the camera lens.

Bart Cannon
Cannon Microprobe
Seattle
bart-at-cannonmp.com



From daemon Tue May 1 17:46:50 2001



From: A.K.Kodd-at-stud.tue.nl ()
Date: Tue, 1 May 2001 17:44:26 -0500
Subject: Ask-A-Microscopist:Microscopy of Bone

Contents Retrieved from Microscopy Listserver Archives
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Email: A.K.Kodd-at-stud.tue.nl
Name: Koen Kodde

Organization: Technical University of Eindhoven

Education: Graduate College

Location: Eindhoven, Noord braband, The Netherlands

Question: L.S.
I'm doing a survey on bone tissue under the microscope. I want to
make the bone tissue visible from a large overview with a regular
microscope to a small overview with a (E)SEM. Do you have any
experience with this kind of survey's. What kind of problems can I
run into (e.g. How can I mark the piece of bone so that I always will
see the same spot of bone under the different microscopes?). Do you
have reports of other students that have done a study alike this one.
At forehand thanks for your time
cheers
koen kodde
student medical engineering


---------------------------------------------------------------------------


From daemon Tue May 1 17:57:56 2001



From: Mark V. Reddington :      mark-at-resolve3d.com
Date: Tue, 01 May 2001 15:55:21 -0800
Subject: help with sectioning

Contents Retrieved from Microscopy Listserver Archives
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We are seeking help with the following problem.

We are doping plastics with high concentrations of dyes and would like
to determine the optical transmission properties of these plastics in
the wavelength range 350-800nm as a function of thickness of the doped
plastic. We do not have the capability to accurately cut thin sections
of these plastics to perform these measurements. Ideally we would like
to have sections cut at 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5,
4.0, 4.5 and 5.0 microns at for a series of dye concentrations in these
plastics and have the sections mounted on glass slides. The sections
should be free of holes, scratches or other defects. We can cast the
plastic to whatever shape is needed but the other dimensions need to be
at least 5x5mm.

Is there a service or contract lab out there that can do the sectioning.
Interested parties should contact me to discuss further details.

Mark

-- ****************************************************
Mark Reddington, Ph.D., Senior Scientist
Resolution Sciences Corporation - http://www.resolve3d.com
500 Tamal Plaza, Corte Madera, CA 94925
Phone: 415 750 6296, fax: 415 750 2332
mreddington-at-resolve3d.com




From daemon Tue May 1 21:47:42 2001



From: sumalee.uthaithavorn-at-philips.com
Date: Wed, 2 May 2001 10:40:50 +0800
Subject: LM: Lens Help, Please (Warning: mundane)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pleases do not sent to me
SU
---------------------- Forwarded by Sumalee Uthaithavorn/BKK/BE/PHILIPS on 2001-05-02 09:43 ---------------------------


milesd-at-US.ibm.com on 2001-05-02 07:33:29
To: Microscopy-at-sparc5.microscopy.com-at-SMTP
cc:


Hello All.

Being an electronics technician, I only know enough about optics
to be dangerous, but use microscopes often. I am trying to
understand what we have, in order to figure out what else could
help us out. I would appreciate information for a "layman", or
pointers web sites that might enlighten me.

First, I need help understanding the markings on our objective
lenses. The question marks indicate what I don't even have
an inkling of the meaning.

#1: Zeiss (The maker of our LSM &
this lens)
Epiplan-NEOFLUAR ( ? )
100x/0,90 (magnification/numerical
aperture)
44 23 80 ( ? )

#2: Olympus
MDPlan 150 ( ? , magnification)
0.95 (numerical aperture)
(infinity sign)/0 f=180 ( ? )

#3 research devices (The maker)
infrared (illumination designed
for)
Trans 100 IRN ( ? ; mag ; near IR ? )
0.90 ( NA )

Is it possible to calculate the working distance from the available
information?

When a lens is made for infrared use, what is different about it?
Are the lens coatings different, or totally absent? Is the lens glass
special?

If you read this far, thank you! I thought I would find the answers to
these questions, and many more, on an episode of "Soap," but
it didn't work. The list is a wonderful pool of knowledge, so don't
fail me now!

Thank you,
Darrell






From daemon Tue May 1 21:51:10 2001



From: Tang Ee Koon, Catherine :      cat_tang-at-nus.edu.sg
Date: Wed, 2 May 2001 10:46:49 +0800
Subject: Cryo TEM of virus

Contents Retrieved from Microscopy Listserver Archives
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Greetings to Prof Holland Cheng, Prof Alex Hyatt and all list users.


I worked in a multi-users laboratory and we have facilities equipped for
cryo TEM. I have been asked by my colleague to post the following questions:

1. Is it necessary to chemically fixed virus-infected samples for cryo TEM?
2. What are the methods and precautions for cryo TEM of unfixed
virus-infected cells?
2. How to dispose the LN2?
3. What are the differences between chemically fixed and unfixed
virus-infected samples for cryo TEM?

Thanks in advance.


Regards
Catherine
EM Unit, NUS



From daemon Tue May 1 23:33:56 2001



From: Heidi Taylor :      heidi.taylor-at-adelaide.edu.au
Date: Wed, 02 May 2001 14:12:38 +0930
Subject: Histocryl resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LM -- Histocryl resin embedding of dried plant material.Has anyone been
successful?


From daemon Wed May 2 02:53:27 2001



From: :      ee.eliminator.org
Date: Wed, 2 May 2001 17:04:49
Subject: Important Internet Revelations....................................................

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I saw your post and thought you might be interested in this...

When you access the Internet, your computer keeps permanent
hidden records of your activities!
I recently tried EE and I was shocked at what it uncovered on my
hard drive.....It actually frightened me. It showed all that I
had been doing even though I had deleted it. My advice is to
check it out NOW
I found it at http://ee1.20m.com
Regards,
Harry










From daemon Wed May 2 10:43:51 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 02 May 2001 10:37:03 -0500
Subject: Re: Ask-A-Microscopist:Microscopy of Bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If the ratio in magnifications between steps is only about 3x, you should
be able to easily locate common features between successive pictures. You
should have some overlap in magnification between your regular microscope
and your ESEM. If not, I am sure you should not have more than a 3-fold
difference. We often take an overview picture with a macro camera of
concrete samples before putting them into the SEM. That is usually
stretching a bit because we are going from 1x for our overview to 20x in
the SEM and it is much harder to locate the same areas over that big a jump.

Of course, if you could scribe some marks outside the bone, that can
provide you with registration points.

Warren

At 05:44 PM 5/1/2001 -0500, you wrote:

} Email: A.K.Kodd-at-stud.tue.nl
} Name: Koen Kodde
}
} Organization: Technical University of Eindhoven
}
} Education: Graduate College
}
} Location: Eindhoven, Noord braband, The Netherlands
}
} Question: L.S.
} I'm doing a survey on bone tissue under the microscope. I want to make the
} bone tissue visible from a large overview with a regular microscope to a
} small overview with a (E)SEM. Do you have any experience with this kind of
} survey's. What kind of problems can I run into (e.g. How can I mark the
} piece of bone so that I always will see the same spot of bone under the
} different microscopes?). Do you have reports of other students that have
} done a study alike this one.
} At forehand thanks for your time
} cheers
} koen kodde
} student medical engineering

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Wed May 2 10:51:54 2001



From: red10-at-cam.ac.uk
Date: Wed, 02 May 2001 16:47:27 +0100
Subject: Developments in FEGTEM III, Oxford University

Contents Retrieved from Microscopy Listserver Archives
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****** Call for contributions ******

Developments in FEGTEM III    

A meeting organised by the Royal Microscopical Society and supported by
EMAG, FEI UK Ltd, JEOL (UK) Ltd.,LEO EM Inc., Gatan UK, TVIPS GmbH

Department of Materials, Oxford University

Tuesday 3 July 2001

The past three years have seen a remarkable growth in field-emission-gun transmission electron microscopy (FEGTEM). Following the pattern of the two
previous events, this meeting will focus both on novel instrumentation developments and on applications to a wide range of problems in the life sciences and the physical materials sciences.

There will be a small number of invited speakers, including

Dr Steven Fuller (Oxford University)
Dr Owen Saxton (Cambridge University)
Dr Rik Brydson (Leeds University) 

representing these aspects of the subject.

The majority of the programme will be devoted to contributed papers. Contributions on all aspects of FEGTEM are now being sought. Abstracts should be sent as soon as possible (and in any case not later than 31 May 2001) to john.hutchison-at-materials.ox.ac.uk, from whom further information may be obtained.

A downloadable registration form, are available at:
http://www.rms.org.uk/current%20events.html#fegtem

Further details are available from the organisers:
Dr John Hutchison, tel +44 (0)1865 273705, john.hutchison-at-materials.ox.ac.uk
Dr Rafal Dunin-Borkowski, tel +44 (0)1223 334564, rafal.db-at-msm.cam.ac.uk

Note. This meeting is timed to precede immediately the one-day EFTEM meting
to be held on 4 July 2001, also in Oxford.


***************************************************************


Rafal Dunin-Borkowski
Department of Materials Science
University of Cambridge
Pembroke Street
Cambridge CB2 3QZ, UK
Tel +44 1223 334564 rafal.db-at-msm.cam.ac.uk
Fax +44 1223 334567 http://www-hrem.msm.cam.ac.uk/~rdb/



From daemon Wed May 2 10:52:12 2001



From: NPGSlithography-at-aol.com
Date: Wed, 2 May 2001 11:47:49 EDT
Subject: Re: Jeol stubs

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 5/1/2001 3:59:27 PM Mountain Daylight Time,
thomasf-at-AGC.BIO.NS.CA writes:

} Just a general question for the List - are those 10mm x 10mm cylindrical
} copper (or Al) Jeol type stubs still in wide usage in the SEM community? Do
} newer Jeol machines still use them?

The JEOL 5900, which is currently their main conventional model, uses a 3/8"
x 3/8" (9.5 mm x 9.5 mm) stub. The older JEOL 840 and 6000 series typically
use a 12.3 mm x 12.3 mm stub.

I quickly checked a few old catalogs and found the prices to range from about
USD $15 to $100 for 100 of these types of Al stubs (the 9.5mm stubs seem to
be about 1/2 the price of the larger size).

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Wed May 2 11:19:26 2001



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Wed, 2 May 2001 09:13:59 -0700 (PDT)
Subject: Wanted: Used JEOL 2010 TEM

Contents Retrieved from Microscopy Listserver Archives
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If anyone knows of a JEOL 2010 TEM available for purchase in good
condition please contact me.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195

(206)543-7702
FAX: (206)685-0403
joswiak-at-astro.washington.edu



From daemon Wed May 2 12:19:37 2001



From: Nathan Haese :      nathan_haese-at-compuserve.com
Date: Wed, 2 May 2001 13:13:13 -0400
Subject: Magnetic Resonance Microscopy of Polymers: May 3rd in Santa

Contents Retrieved from Microscopy Listserver Archives
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****************** ****************** ****************** ******************
******************

SOCIETY FOR APPLIED SPECTROSCOPY - Northern California Section

****************** SAS NATIONAL TOUR SPEAKER MEETING ******************

DATE: Thursday, May 3rd, 2001

SPEAKER: ROBERT BOTTO - Argonne National Laboratory

TITLE: "Molecular Architecture of Polymers by Magnetic Resonance
Microscopy"

LOC: David's Restaurant, Santa Clara CA (Directions to follow)

Dinner: 7:00 PM
Talk: 8:00 PM
Cost: $30, free for talk.

Dinner Choices: (Indicate Choice when RSVP'ing)
Red Snapper, Basque Style
London Broil
Grilled, Fresh Vegetables & Smoked Mozzarella with Herbs in Puff
Pastry

RSVP: On-line at http://www.amplifyllc.com/go/ncss. Preferred method.
(Can use credit cards.)
Or, email steve.rabin-at-alza.com.
Or, call Steve Rabin at 1-650-564-5315

PLEASE RSVP BY MONDAY APRIL 30th.

(Please let us know if you're coming for dinner, or just the talk for
headcount purposes)

****************** ABSTRACT ******************

Magnetic resonance microscopy (MRM) has been employed to investigate
solvent transport phenomena in glassy and rubbery macromolecular systems.
Time-dependent proton or fluorine imaging of solvent uptake allows one to
distinguish between two different transport mechanisms. An exponential
solvent concentration profile observed in the case of rubbery networks is
consistent with Fickian behavior. In glassy systems, however, where solvent

induces a glass-to-rubber phase transition of the network, an extremely
sharp
solvent front is observed which propagates through the specimen as a shock
wave at constant velocity which is typical of anomalous transport. MRM
analysis forms the basis of a model of anomalous transport in
macromolecular
solids which couples diffusion of solvent with kinetics of the phase
transition of the network. Analysis of solvent transport kinetics, front
velocities, molecular diffusion constants and localized chain


****************** DIRECTIONS TO DAVID'S RESTAURANT ******************

David's Restaurant,
5151 Stars and Stripes Drive (off Tasman Dr., Santa Clara CA, at the Santa
Clara Golf club)

} From the East Bay:
Take 680 or 880 to Highway 237 toward Mountain View.
Stay on 237 until the Great America Parkway exit.
Follow Great America Parkway to Tasman Dr, take a left on Tasman.
Approximately 0.5 miles on the left, turn left onto Centennial Drive.
David's will be in front of you.

} From San Jose
Take 101 North to Great America Parkway.
Take Great America Parkway to Tasman Drive, turn right on Tasman.
Approximately 0.5 miles on the left, turn left onto Centennial Drive.
David's will be in front of you.

} From the Peninsula
Take 101 South to Highway 237 toward Milpitas.
Exit at Great America Parkway.
Follow Great America Parkway to Tasman Dr, take a left on Tasman.
Approximately 0.5 miles on the left, turn left onto Centennial Drive.
David's will be in front of you.

****************** ****************** ****************** ******************



From daemon Wed May 2 13:10:54 2001



From: Jim Nicolino :      JNicolino-at-xraydetectors.com
Date: Wed, 02 May 2001 14:06:10 -0400
Subject: EDX Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear List,
I have noticed some recent inquiries about resolution changes with
Energy Dispersive Spectrometers and also the reported
solutions. There seems to be some misinformation about the use and
maintenance of these detectors.
These detectors are basically a closed vacuum chamber which over time
can have water vapor and other gases enter the
system through the entrance window in the case of Light Element Windows
or general out gassing due to deterioration of o-ring
seals etc. When water vapor is present in the system it will collect on
the cold finger of the detector and as such will create an
ice layer on the Si(Li) crystal since it is also at near LN2
temperature. When this occurs, resolution of the detector degrades.
One of the solutions reported was to allow the detector to warm up to
room temperature and clean the dewar. Unfortunately
this will only allow the process to reoccur in a short period of time.
What is necessary is a thorough pump-out and bake-out of
the detector after replacing various vacuum seals. This can only be
accomplished by experienced technical personnel with the
appropriate vacuum pump station. Obviously one can return the detector
to the original manufacturer or some other repair
facility.
My company, X-Ray Optics/AAT is one of these EDX repair facilities and
if anyone is interested they may contact me through
my web site at www.xraydetectors.com
I hope this information is useful to the members of the listserver.
Regards,
Jim Nicolino


From daemon Wed May 2 13:57:22 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 2 May 2001 14:55:10 -0400
Subject: NESM's 18th Annual Woods Hole Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

Just a reminder--there is still time to pre-register (deadline Friday, May 4th)
for the 18th Annual Woods Hole Symposium, May 11-12th at Woods Hole
Oceanographic Institute, Woods Hole, MA. This meeting is sponsored
by the New England Society for Microscopy (NESM).

This meeting not only includes excellent biological and materials
science speakers, but a symposium on "Remote Access Microsocpy" on
Saturday morning.

I would like to direct listserver members to NESM's website which can
be accessed directly (see below) or through the MSA website under
affiliate societies.
The url is: http://www.msa.microscopy.com/MSALAS/NESM/

Complete information re: the above mentioned symposium,including the
program, plus registration forms and contacts can be found on this
website under April Newsletter.

Hope to see many of you there!

Peggy Sherwood
Corresponding Secretary, NESM
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed May 2 15:15:17 2001



From: L. Muffley :      muffley-at-u.washington.edu
Date: Wed, 2 May 2001 13:08:49 -0700 (PDT)
Subject: PAS staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello- I am planning a periodic acid - Shiff stain for basement membrane
in mouse skin. I understand there are two methods - one with alcoholic
solutions and one with aqueous solutions. Does anyone have experience with
this stain, and could you advise me in the choice of method? Any other
advice would be quite welcome as well.
Thanks!

Lara Muffley
Dermatology Dept
University of Washington
Seattle, WA
muffley-at-u.washington.edu



From daemon Wed May 2 15:55:01 2001



From: Paul Anderson :      paanders-at-lynx.dac.neu.edu
Date: Wed, 2 May 2001 16:50:23 -0400 (EDT)
Subject: JEOL/gatan specimen holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


sample holders for JEOL 100CX-2000EXII model TEMs for sale:

gatan hot stage sample holders. both furnaces in working order; holders
include one hexnut lockring AND thermocouple controller (sorry, no power
wires--lost!):
--#1 in good condition, $9000 OBO (gatan power supply model #580-0300)
--#2 lightly stripped hex nut assembly (doesn't tighten all the way) in
furnace, mA display on power supply (gatan power supply model #628-0500)
a bit jumpy but TC reads temperature fine, $4000 OBO

extra washers (gatan part #628-0223) and hex nut tool (gatan part
#608-0005) NOT included with the above holders.

JEOL EM-SCSH
--common bulk specimen holder (STEM) with graphite retainer, $900

please contact Terry Baker for further inquiries and negotiations:
phone 508 893 9560
baker-at-catalytic-materials.com




From daemon Wed May 2 21:05:52 2001



From: sumalee.uthaithavorn-at-philips.com
Date: Thu, 3 May 2001 09:59:52 +0800
Subject: wrong mail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dera Postmaster
You sent the wrong mail to wrong person. Pleases do not mail to me. My mail box are full.
Kindly delete my name out of your groups mail.
Thanks postmaster
Best Reagrds,
SU


From daemon Thu May 3 00:59:41 2001



From: dinesh-at-astra.iisc.ernet.in ()
Date: Thu, 3 May 2001 08:10:19 -0500
Subject: Ask-A-Microscopist: fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Neuhaus, who contacted MSA and offered to donate a vintage light
microscope, informs us that the instrument is on its way to a new home.
See below.

I sense that he was a tiny bit overwhelmed by the size of the positive
response he received.

Thanks, listers!!

Ron


Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg
---------------------- Forwarded by Ronald Anderson/Fishkill/IBM on
05/02/2001 10:49 AM ---------------------------

"g. neuhaus" {ulmithaca-at-home.com} on 04/29/2001 06:47:28 PM

To: Ronald Anderson/Fishkill/IBM-at-IBMUS
cc:


Below is the result of your feedback form. It was submitted by
(dinesh-at-astra.iisc.ernet.in) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 3, 2001 at 05:19:42
---------------------------------------------------------------------------

Email: dinesh-at-astra.iisc.ernet.in
Name: Dinesh

Organization: Indian Institute of Science

Education: Graduate College

Location: Banagalore, Karnataka, India

Question: What is the wavelength and excitation required for
identification of methane bacteria using fluorescence microscope.

---------------------------------------------------------------------------


From daemon Thu May 3 09:39:52 2001



From: JHoffpa464-at-aol.com
Date: Thu, 3 May 2001 10:34:11 EDT
Subject: tech time and cost analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--part1_2b.14d2d65e.2822c663_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

Anyone done a tech time and cost analysis lately. Looking to compare notes.

John Hoffpauir
Cooper hospital

--part1_2b.14d2d65e.2822c663_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} Anyone done a tech time and cost analysis lately. Looking to compare notes.
{BR}
{BR} John Hoffpauir
{BR} Cooper hospital {/FONT} {/HTML}

--part1_2b.14d2d65e.2822c663_boundary--


From daemon Thu May 3 11:27:35 2001



From: David Chiluiza :      david.chiluiza-at-usm.edu
Date: Thu, 3 May 2001 11:25:49 -0500
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe


__________________________________________________
David Chiluiza
USM, Institute of Marine Sciences
Gulf Coast Research Laboratory
703 East Beach Drive, Ocean Springs, MS 39566-7000

Phone: (228) 872-4287, Fax: (228) 872-4204
Email: david.chiluiza-at-usm.edu
Homepage: http://www.ims.usm.edu/students/gchiludc.htm
__________________________________________________



From daemon Thu May 3 12:24:07 2001



From: Audette, David E. :      david.audette-at-sylvania.com
Date: Thu, 3 May 2001 13:17:15 -0400
Subject: EBSD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow listers,

Has anyone had experience with mounting an electron backscatter diffraction
camera on an Amray FESEM 1845 round chamber? There is an EDS spectrometer
already mounted on the chamber opposite the stage on the tilt side. This
appears to leave a small port if the apertures can be relocated or a larger
one if a fiber optic is feasible.

Thanks,

Dave Audette

david.audette-at-sylvania.com


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end


From daemon Thu May 3 13:44:03 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 3 May 2001 11:35:40 -0700
Subject: Early TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A friend of mine who makes excellent educational videos asked me a question
today that I couldn't answer offhand. Will some old-timer please help my
fading memory?

} ...when were the first EMs of chloroplasts (showing grana) produced? My
} vague } guess was around 1949, but I can't find a definitive reference.

Please copy your response directly to him: bruce russell
{biomedia-at-mail.telis.net}

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu May 3 13:59:42 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 03 May 2001 13:54:43 -0500
Subject: Re: EBSD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am not at all familiar with the 1845. Way back when, we put BSE on our
old JEOL U3. There were four solid state detectors that were fastened to
the bottom of the pole piece with double stick tape. We also put BSE on our
JEOL 840A. That was JEOL's system and it also fit to the bottom of the pole
piece. Now we have a Robinson on a Hitachi 2460N which comes in from the
side. We also have Oxford's Tetra with an array of detectors. Ours is set
to slide in when needed.

I don't know why you couldn't mount a detector permanently to the pole
piece and wire it to a feed thru on any port. Hopefully the BSE detector
folks could work with you on this.

At 01:17 PM 5/3/2001 -0400, you wrote:

} Fellow listers,
}
} Has anyone had experience with mounting an electron backscatter diffraction
} camera on an Amray FESEM 1845 round chamber? There is an EDS spectrometer
} already mounted on the chamber opposite the stage on the tilt side. This
} appears to leave a small port if the apertures can be relocated or a larger
} one if a fiber optic is feasible.
}
} Thanks,
}
} Dave Audette
}
} david.audette-at-sylvania.com



From daemon Thu May 3 22:09:45 2001



From: yarrabee-at-caboolture.net.au ()
Date: Thu, 3 May 2001 22:04:05 -0500
Subject: Ask-A-Microscopist: isolating mitchondria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(yarrabee-at-caboolture.net.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 3, 2001 at 19:52:32
---------------------------------------------------------------------------

Email: yarrabee-at-caboolture.net.au
Name: Eliza

Organization: QUT

Education: Undergraduate College

Location: Brisbane, QLD, Australia

Question: Hello. I was wondering if someone could tell me what the
best method is of isolating mitchondria from rat liver cells for TEM
analysis?

---------------------------------------------------------------------------


From daemon Fri May 4 08:49:18 2001



From: barkdoll-at-acadia.net ()
Date: Fri, 4 May 2001 08:38:18 -0500
Subject: Ask-A-Microscopist:Questions on high quality photomicrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(barkdoll-at-acadia.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 3, 2001 at 23:43:49
---------------------------------------------------------------------------

Email: barkdoll-at-acadia.net
Name: Edwin Barkdoll

Organization: Small Animal Clinic

Education: Graduate College

Location: Ellsworth, ME, USA

Question: I hope these questions are appropriate for this
forum.

I am trying to planning to take high quality photomicrographs using a
steromicroscope. I currently use a Russian made microscope and SLR
body connected to an eyepiece tube. I have not been happy with this
setup because of a noticeable degradation of image quaility towards
the edges and difficulty of use. I am looking into upgrading to a
system with fewer of these problems.

I have looked at literature from Nikon (SMZ series) and Olympus (SZ,
SZH series) and received some quotes from distributors, however I am
not tied to these manufacturers.

My questions are:
1) is there any source for "reviews" or published tests of particular
microscopes?
2) how marked a difference should I expect between PLAN and PLAN-APO
objectives?
3) my SLR does not have mirror lock-up - I'm willing to get a 35 mm
back if warranted - how significant is the lack of MLU with an SLR an
image quality?
4) some of the scopes are not inexpensive - what are some good ways
for me to avoid getting a something that really exceeds my needs in
image quality?

Thank you very much,
Edwin Barkdoll V.M.D, Ph.D.

---------------------------------------------------------------------------


From daemon Fri May 4 09:12:10 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Fri, 4 May 2001 10:04:25 -0400
Subject: RATS R US

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eliza:

You have asked how to isolate rat liver mitochondria for TEM analysis. I
ask why do you want to isolate them? If your interest is just in the
ultrastructrure of the mitochondria themselves, then the easiest thing to do
is fix and process the whole tissue. The liver is absolutely loaded with
mitochondria and you will have plenty of them to look at. If your interest
is more specific, then please elaborate on your project. Good luck in
your endeavors, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Fri May 4 09:21:45 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Fri, 04 May 2001 10:20:27 -0400
Subject: Re: Retina and collagen protocols

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tim,

The enucleated eyes must be punctured at the ora serrata with a razor blade and then fixed by immersion in a mixture of 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer at pH 7.3 for 4-5 h. After removal of the cornea and lens the eyes are then rinse overnight in 0.1 M sodium cacodylate buffer containing 3% sucrose and post fixed in 2% osmium tetroxide in the same buffer for 2h at room temperature in the same buffer before dehydration and so on.

Ref: W.C. Yang, M.L. Hollenberg, and J.P.H. Wyse. Morphology of the retinal pigment epithelium in the vitamin A deficient rat. Virchows Arch. B Cell Path. 27, 7-21(1978)



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Quinn, Tim Lee" {tquinn-at-ku.edu} 04/30 10:22 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fellow microscopists:

Does anyone have a good protocol for retina fixation for TEM?

I'm dealing with specimens collected in the field and it appears that the
initial field fix- "cacodylate and glut" did not preserve the "rods and
cones".

A good recomendation for a field fix would be appreciated. Would Karnovsky's
work?

I also need to fix frog skin tissue, causing minimal shrinkage. Any
suggestions?

Thanks

Tim Quinn




From daemon Fri May 4 09:54:15 2001



From: steven wintonick :      crimsem-at-hotmail.com
Date: Fri, 04 May 2001 10:48:45 -0400
Subject: EMSA Certifcation???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I just recently read a job announcement, and it included that an EMSA
certification would be preferred. Through some inquiries, I found that EMSA
is the Electron Microscopy Society of America and I obtained a phone number
for them (In Mass). However, I've searched the web and many microsopy sites
and there seems to be no mention of this organization anywhere. This, along
with the phone number being a private residence, leads me to believe this
society has either renamed themselves or has disbanded. I was wondering if
anyone has any information on ESMA or any other organization that offers a
professional certification program for electron microscopy. If there are
programs, I would also appreciate a critique on which are the best, and if a
certification is even necessary for careers in electron microscopy. Any and
all info is very helpful. Thanks.

Steve Wintonick


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com



From daemon Fri May 4 14:21:44 2001



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Fri, 4 May 2001 20:27:59 +0100 (CAT)
Subject: Routine Maintenance of SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Guys,
Can anyone give a list of activities that constitute routine maintenance
of an SEM particulaly a LEO 1450 model with only secondary electron
detector.
Thanks
Soji

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.

email: sojilori-at-oauife.edu.ng



From daemon Fri May 4 14:51:25 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 04 May 2001 12:50:22 -0700
Subject: Fwd: RATS R US

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may isolate mitochondria from homogenated tissue by using Percoll
gradient. It's actually one step procedure. Percoll is iso-osmotic,
it'll prevent mitochondria from damage. I don't remember details, but you
have to mix your homogenate with buffer and Percoll in some proportion and
centrifuge at low speed for about an hour. Than you will see the reddish
mitochondria layer. You may remove Percoll by centrifugation at higher
speed if necessary. I did some neg-staining of mitochondria couple of
years ago. If you need detailed protocol, let me know, I have to check my
old records.

Sergey.


} Date: Fri, 04 May 2001 10:04:25 -0400
} From: Timothy Schneider {Timothy.Schneider-at-mail.tju.edu}
} Subject: RATS R US
} To: microscopy-at-sparc5.microscopy.com
} Reply-to: Timothy.Schneider-at-mail.tju.edu
} X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2910.0)
} Importance: Normal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri May 4 14:51:25 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 4 May 2001 14:45:39 -0500
Subject: Re: Ask-A-Microscopist:Questions on high quality photomicrographsusing a steromicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you are working with magnification of less the 80x you will probably
have better luck with an extention bellows and a reversed Kodak Ektar Cine
lens.

It will be a great deal easier on you pocket book as well.

Our eyes ignore a great deal of distortion that a camera quickly catches.

If you decide to buy a new scope or one new to you either test it at the
dealers or buy it with the understanding that you can return it if it
doesn't perform as you expect.

Stereo microscopes are not very good platforms for photography.
Conventional macro photography methods are usually much better though less
convineint.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

}
} Question: I hope these questions are appropriate for this
} forum.
}
} I am trying to planning to take high quality photomicrographs using a
} steromicroscope. I currently use a Russian made microscope and SLR
} body connected to an eyepiece tube. I have not been happy with this
} setup because of a noticeable degradation of image quaility towards
} the edges and difficulty of use. I am looking into upgrading to a
} system with fewer of these problems.
}
} I have looked at literature from Nikon (SMZ series) and Olympus (SZ,
} SZH series) and received some quotes from distributors, however I am
} not tied to these manufacturers.
}
} My questions are:
} 1) is there any source for "reviews" or published tests of particular
} microscopes?
} 2) how marked a difference should I expect between PLAN and PLAN-APO
} objectives?
} 3) my SLR does not have mirror lock-up - I'm willing to get a 35 mm
} back if warranted - how significant is the lack of MLU with an SLR an
} image quality?
} 4) some of the scopes are not inexpensive - what are some good ways
} for me to avoid getting a something that really exceeds my needs in
} image quality?
}
} Thank you very much,
} Edwin Barkdoll V.M.D, Ph.D.
}
} ------------------------------------------------------------------------
---
}




From daemon Fri May 4 18:00:01 2001



From: Louise_Harner-at-albint.com
Date: Fri, 4 May 2001 18:53:48 -0400
Subject: Re: EMSA Certifcation???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Steve -

Congratulations! You've already found "EMSA" even if you didn't realize it. EMSA
became MSA, the Microscopy Society of America, a few years ago (1993? My, how
time has flown!). You're already on the MSA listserver. The society homepage is
at:

http://www.msa.microscopy.com/

For information on MSA certification, you can follow the links from MSA's
homepage or from:

http://www.cvmbs.colostate.edu/emcenter/msa/certboard/

Certification is NOT necessary for a career in electron microscopy, but it may
be required for a specific job opening. It does offer prospective employers some
confirmation that you have the necessary background knowledge and skills to
safely and successfully play with their expensive toys. The MSA certification is
directed more toward biological microscopy (specifically biological TEM) than
materials science microscopy.

Participation in some of the microscopy courses and training opportunities
mentioned/promoted on the Listserver could also serve as assurance to
prospective employers. I believe there are at least two colleges that offer
degrees or certifications specifically in electron microscopy (one in California
& one in Wisconsin???). Then there are places such as McCrone Research Institute
that offer specialty microscopy degrees (e.g. Chemical Microscopy Certification
at http://www.mcri.org/) which may include electron microscopy components along
with light microscopy techniques.

There are also a few jobs like 'SEM technician - high school diploma required,
SEM experience preferred' - a current, shiftwork job posting here in
Massachusetts. Somehow I doubt that 'microscopist' will have any upward mobility
unless he is VERY lucky and/or improves his education level.

Hope this has been helpful. I'm sure you'll get lots of other replies.

- Louise Harner

Louise Harner
Research Microscopist
Albany International Research Co.
777 West Street, P.O. Box 9114
Mansfield, MA 02048
508-339-7300 phone
508-339-4996 fax
Louise_Harner-at-albint.com





"steven
wintonick" To: Microscopy-at-sparc5.microscopy.com
{crimsem-at-hotm cc:
ail.com} Subject: EMSA Certifcation???

2001/05/04
10:48 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I just recently read a job announcement, and it included that an EMSA
certification would be preferred. Through some inquiries, I found that EMSA
is the Electron Microscopy Society of America and I obtained a phone number
for them (In Mass). However, I've searched the web and many microsopy sites
and there seems to be no mention of this organization anywhere. This, along
with the phone number being a private residence, leads me to believe this
society has either renamed themselves or has disbanded. I was wondering if
anyone has any information on ESMA or any other organization that offers a
professional certification program for electron microscopy. If there are
programs, I would also appreciate a critique on which are the best, and if a
certification is even necessary for careers in electron microscopy. Any and
all info is very helpful. Thanks.

Steve Wintonick


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com







From daemon Fri May 4 18:46:43 2001



From: Ronald Austin :      rla-at-mindspring.com
Date: Fri, 4 May 2001 18:42:55 -0500
Subject: E.M. for FNA's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any procedures to do Fine Needle Aspiration (FNA) for
routine E.M. morphology. I.e.... collect, fix, rinse, dehydrate, infiltrate,
and embed in an epoxy resin.

Ron Austin
Dept of Pathology
LSUMC
Shreveport, LA
rla-at-mindspring.com



From daemon Sat May 5 03:55:23 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Sat, 5 May 2001 04:46:16 -0400
Subject: Routine Maintenance of SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Routine maintenance of an instrument like the LEO falls into the following
areas -

1. Cleaning the cathode assembly each time you change a filament

2. Cleaning the anode every other time you change a filament

4. Using a chamois leather (washed oil free) to wipe round the gun
chamber each time you change a filament

5. Keeping spare apertures for each part of the system (ask the
service engineer or check in the manual).

5. Using a vacuum cleaner to clean up inside the apecimen area every
few weeks.

That should keep you going but if you need more information our interactive
Cd "Monitoring & Maintaining EM Performance" will tell you all you need to
know about how the instrument works, which areas to check, how to check
them and how to clean them if they are found to be a problem.

Good luck

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Sat May 5 10:19:58 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 05 May 2001 08:12:30 -0700
Subject: Ask-A-Microscopist:Questions on high quality photomicrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have not used the Nikon stereo but I have used the Olympus SZ series
and currently use a SZH10. The SZ has integrated objectives but will
accommodate auxillary objectives. This scope is a good unit and is
mostly a plastic-type unit. The SZH10 is metal and uses a screw-in
objective or two objectives on a rotating turret. For most work, I
really can't see any remarkable difference between Plan and PlanAPO
objectives. I use a 1.0X PlanAPO on my SZH10 and 0.5X and 0.75X
Plan objectives. The SZ scopes are rather good and are not
horribly expensive. They do a good job.

I agree that trying take pix with an SLR is not only difficult but
just about impossible--so to speak. The SLR must have a plain
ground glass focusing screen or focusing will be a real challenge.
It is a challenge even with the correct focusing screen.
Mirror lock up and manual exposure is also highly desirable. The
other problem (very subtle and quite frustrating) is that to get
good pix with a stereo zoom, the metering system should be a
spot meter type. The central portion of interest (assuming a
non-uniform subject) will not properly expose (usually is way
over exposed) when metered using normal averaging mode.
So, spot meter, AE lock or manual mode, then mirror lock up
and shoot.

The unevenness you may see on an SLR pix is due to vignetting
as a result of the adapter between camera and scope. A different
adapter should correct this.

If you are looking for truly high quality pix and want to do this
with ease, I found that a camera system optimized for microphotography
is necessary. For the Olympus line, the PM10-ADS is an automatic
camera system with a leaf shutter. With this system, there is no
shake at all. Unfortunately, I believe that this system has been
discontinued. Their PM10-AD is an averaging meter system
and is also discontinued. The PM-20 and PM-30 are newer systems
and are quite expensive ($12K-$25K I think).

For digital cameras, I found that the Pixera units are outstanding.
I use the Penguin 600CL. It is a cooled 5.8M pixel (2776x2074) camera which
takes 17MB RGB TIFF and other format images in perfect rendition.
This camera costs about $8500. The un-cooled version costs about
$6600. The 1.5M pixel model costs about $6600. Their 1.2M pixel
un-cooled camera (150ES) costs about $3700. These are complete
camera systems which include a PCI card, real-time focusing interface,
image capture and image viewing software for PC or Mac.

If you would like more information about any of these units, please
contact me off-line.


Gary Gaugler, Ph.D.
Optical Reflections
7970 Twin Rocks Rd
Granite Bay, CA 95746-8111
916.791.8191
916.791.8186 fax

Disclaimer: I am an authorized dealer for these and other photographic
and software products.

http://photoweb.net


At 06:38 AM 5/4/2001, you wrote:


Below is the result of your feedback form. It was submitted by
(barkdoll-at-acadia.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday, May 3,
2001 at 23:43:49
---------------------------------------------------------------------------

Email: barkdoll-at-acadia.net
Name: Edwin Barkdoll

Organization: Small Animal Clinic

Education: Graduate College

Location: Ellsworth, ME, USA

Question: I hope these questions are appropriate for this
forum.

I am trying to planning to take high quality photomicrographs using a
steromicroscope. I currently use a Russian made microscope and SLR body
connected to an eyepiece tube. I have not been happy with this setup
because of a noticeable degradation of image quaility towards the edges and
difficulty of use. I am looking into upgrading to a system with fewer of
these problems.

I have looked at literature from Nikon (SMZ series) and Olympus (SZ, SZH
series) and received some quotes from distributors, however I am not tied
to these manufacturers.

My questions are:
1) is there any source for "reviews" or published tests of particular
microscopes?
2) how marked a difference should I expect between PLAN and PLAN-APO
objectives?
3) my SLR does not have mirror lock-up - I'm willing to get a 35 mm back if
warranted - how significant is the lack of MLU with an SLR an image quality?
4) some of the scopes are not inexpensive - what are some good ways for me
to avoid getting a something that really exceeds my needs in image quality?

Thank you very much,
Edwin Barkdoll V.M.D, Ph.D.

---------------------------------------------------------------------------



From daemon Sun May 6 13:27:55 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sun, 6 May 2001 13:13:27 -0500
Subject: MM-2001 Meeting Program Search Engine is Now Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Microscopy and Microanalysis -2001 Program Search Engine
is now on-line.

Using this search engine you can determine the time, location of
and title of any presentation during the August Meeting in Long Beach.

You may reach this from the MSA WWW Site

http://www.msa.microscopy.com

then follow the links to the Microscopy & Microanalysis 2001
Meeting. You can also use it to
view a detailed program listing for any day of the week, prior
to receiving your hard copy of the program.

Cheers...

Nestor
Your Friendly Neighborhood SysOp


From daemon Sun May 6 18:47:17 2001



From: David Chiluiza :      david.chiluiza-at-usm.edu
Date: Sun, 6 May 2001 18:46:58 -0500
Subject: seeking a summer course on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regards to everyone.
I am a graduate student at University of Southern Mississippi and I am
seeking for a summer course in Scanning electron microscopy, preferably
focused in biological analysis (I wook with fish larvae).
If someone have knowledge of any course, please, let me know, I will thanks
any information.
Sincerely,
David Chiluiza


__________________________________________________
David Chiluiza
USM, Institute of Marine Sciences
Gulf Coast Research Laboratory
703 East Beach Drive, Ocean Springs, MS 39566-7000

Phone: (228) 872-4287, Fax: (228) 872-4204
Email: david.chiluiza-at-usm.edu
Homepage: http://www.ims.usm.edu/students/gchiludc.htm
__________________________________________________



From daemon Mon May 7 01:22:15 2001



From: Petra Wahlbring :      petra.wahlbring-at-nexgo.de
Date: Mon, 7 May 2001 08:02:32 +0200
Subject: Prof. Dr. L. Reimer died on 29. April 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I think some of you will have an interest in the following, very sad news:

Prof. Dr. Ludwig Reimer (Muenster, Germany) died on April 29th, 2001.

I don't know how to express this correctly in english, but I feel it as a
very big loss. I will leave it to others to review his scientific
contributions to the community, I know him best as teacher to his students
to whom he was always devoted.

Just wanted to let you know...

Petra Wahlbring
-------------------------------------

Dr. Petra Wahlbring
Centre de Recherche Gabriel Lippmann
Laboratoire d'Analyse des Matériaux
162a, avenue de la Faiencerie
L-1511 Luxembourg
petra.wahlbring-at-crpgl.lu



From daemon Mon May 7 07:22:37 2001



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Mon, 07 May 2001 09:15:23 -0300
Subject: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I need to be away for two weeks, and would like opinions
on what is best to do with an EDS detector, LN2-wise, while
away. My options appear to be:

1. Leave everything on as normal. Don't really want to do this - I have
someone I trust to keep the LN2 dewar full, but not really handle the
microscope if something like a power failure or worse occurs.

2. Leave scope off, keep LN2 in EDS, hope residual vacuum in SEM
is adequate for two weeks. I'm guessing it's best to power off the
detector and computer, regardless.

3. Power down EDS, running detector through it's conditioning cycle,
leave everything off and at room temperature.

4. Stay home, chained to the system.

Thoughts, experiences greatly appreciated.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Mon May 7 08:15:50 2001



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Mon, 7 May 2001 09:10:33 -0400
Subject: Prof. Dr. L. Reimer died on 29. April 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's truly a big loss to electron microscopy community. Last mid-night, I
just bought the new addition of his famous book "Transmission Electron
Microscopy : Physics of Image Formation and Microanalysis (4th Ed)(Springer
Series in Optical Sciences, Vol 36)" from Amazon.com. Didn't realize that it
would be his last addition of the book.
-cy

-----Original Message-----
} From: Petra Wahlbring [mailto:petra.wahlbring-at-nexgo.de]
Sent: Monday, May 07, 2001 2:03 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Colleagues,

I think some of you will have an interest in the following, very sad news:

Prof. Dr. Ludwig Reimer (Muenster, Germany) died on April 29th, 2001.

I don't know how to express this correctly in english, but I feel it as a
very big loss. I will leave it to others to review his scientific
contributions to the community, I know him best as teacher to his students
to whom he was always devoted.

Just wanted to let you know...

Petra Wahlbring
-------------------------------------

Dr. Petra Wahlbring
Centre de Recherche Gabriel Lippmann
Laboratoire d'Analyse des Matériaux
162a, avenue de la Faiencerie
L-1511 Luxembourg
petra.wahlbring-at-crpgl.lu



From daemon Mon May 7 13:41:09 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 07 May 2001 14:26:10 -0400
Subject: Re: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In your situation, and assuming I absolutely trusted the assistant with
keeping the Liq. N2 up, I would use your option 2. Certainly turn off the
detector and computer.

Tony Garratt-Reed

} Hi Listers,
}
} I need to be away for two weeks, and would like opinions
} on what is best to do with an EDS detector, LN2-wise, while
} away. My options appear to be:
}
} 1. Leave everything on as normal. Don't really want to do this - I have
} someone I trust to keep the LN2 dewar full, but not really handle the
} microscope if something like a power failure or worse occurs.
}
} 2. Leave scope off, keep LN2 in EDS, hope residual vacuum in SEM
} is adequate for two weeks. I'm guessing it's best to power off the
} detector and computer, regardless.
}
} 3. Power down EDS, running detector through it's conditioning cycle,
} leave everything off and at room temperature.
}
} 4. Stay home, chained to the system.
}
} Thoughts, experiences greatly appreciated.
}
} Cheers,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Mon May 7 15:39:27 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 7 May 2001 16:33:20 -0400
Subject: Re: Prof. Dr. L. Reimer died on 29. April 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html








I think some of you will have an interest in the following, very sad news:

Prof. Dr. Ludwig Reimer (Muenster, Germany) died on April 29th, 2001.

I don't know how to express this correctly in english, but I feel it as a
very big loss. I will leave it to others to review his scientific
contributions to the community, I know him best as teacher to his students
to whom he was always devoted.

Just wanted to let you know...

That is, indeed, very sad news. I met Ludwig when I was visiting the CNRS
to learn EELS. Not only was he very helpful professionally, but we also shared
many meals and as trip to Toulouse Latreck's birthplace.
Bill Tivol




From daemon Mon May 7 16:00:00 2001



From: David Chiluiza :      david.chiluiza-at-usm.edu
Date: Mon, 7 May 2001 16:01:11 -0500
Subject: fixation for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

Could anyone help me with a good fixation protocol for marine fish larvae
(1-5mm) for SEM? I have been using glutaraldehyde + Osmium tetroxide and in
bloc staining with Uranyl acetate for TEM but I understand that osmolarity
is a very important factor in SEM.

Do you think I can analyze in SEM the samples fixed for TEM?

Thanks a lot for any help.
David Chiluiza

__________________________________________________
David Chiluiza
USM, Institute of Marine Sciences
Gulf Coast Research Laboratory
703 East Beach Drive, Ocean Springs, MS 39566-7000

Phone: (228) 872-4287, Fax: (228) 872-4204
Email: david.chiluiza-at-usm.edu
Homepage: http://www.ims.usm.edu/students/gchiludc.htm
__________________________________________________



From daemon Mon May 7 16:33:03 2001



From: tbargar-at-unmc.edu
Date: Mon, 7 May 2001 16:29:54 -0500
Subject: Glycol-Methacrylate blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stay home is probably your safest bet.

Earl

----- Original Message -----
} From: "James M. Ehrman" {jehrman-at-mta.ca}
To: "Microscopy Listserv" {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, May 07, 2001 5:15 AM


A faculty member brought me some 10 year old samples embedded in
Glycol-Methacrylate. He would like some thick sections 1-2microns. What's
the best way to section and handle these to get good flat sections? Also
is there any solvent which would dissolve the blocks so that the samples
could be reembedded in another resin? All advice appreciated, thanks

Tom Bargar
EM Lab, UNMC
(402)559-7347



From daemon Mon May 7 19:09:01 2001



From: David Chiluiza :      david.chiluiza-at-usm.edu
Date: Mon, 7 May 2001 19:07:40 -0500
Subject: fixation for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

Could anyone help me with a good fixation protocol for marine fish larvae
(1-5mm) for SEM? I have been using glutaraldehyde + Osmium tetroxide and in
bloc staining with Uranyl acetate for TEM but I understand that osmolarity
is a very important factor in SEM.

Do you think I can analyze in SEM the samples fixed for TEM?

Thanks a lot for any help.
David Chiluiza

__________________________________________________
David Chiluiza
USM, Institute of Marine Sciences
Gulf Coast Research Laboratory
703 East Beach Drive, Ocean Springs, MS 39566-7000

Phone: (228) 872-4287, Fax: (228) 872-4204
Email: david.chiluiza-at-usm.edu
Homepage: http://www.ims.usm.edu/students/gchiludc.htm
__________________________________________________



From daemon Mon May 7 20:02:57 2001



From: Dale J Telgenhoff :      telgenh4-at-pilot.msu.edu
Date: Mon, 7 May 2001 20:58:32 -0400 (EDT)
Subject: Platinum EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Does anyone know a good method for finding platinum in a cell? I am treating
with platinum drugs and was wondering if I could localize platinum binding
sites via TEM EDS analysis. Any help is appreciated!

Thank you,

Dale

--
#####################
Dale J. Telgenhoff #
Zoology Department #
Michigan State U. #
(517) 355-3326 #
telgenh4-at-msu.edu #
#####################

"Give a man a fish and he will eat for a day. Teach him how to
fish and he will sit in a boat & drink beer all day."

-Unknown


From daemon Mon May 7 22:53:18 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Monday, May 07, 2001 6:28 AM
Subject: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To Jim and all:

My answer is 3. Turn it off and let it warm up. Answer 2 is strange because
the dewar vacuum is independent of the SEM vacuum. This what the window is
for. If a windowless detector then no problem with warm up. See below

This answer assumes that system is reasonable new. Why? Because what
happens when the EDS detector warms up is not a detector problem, it is a
vacuum problem. If the vacsorb material inside the dewar warms up and it is
full it will release the adsorbed gases to the dewar vacuum. New detectors
(less than three years old) without obvious vacuum problems or degraded
resolution now days can be warmed up without problems. In fact now days EDS
systems are shipped dry by the manufacturers. We used to ship them cold in
the seventies but that day has past. When I worked for Kevex and before that
at Tracor Xray we saw very few problems when dewars warmed up except some
freak problems. Freak problem did include windows blowing outwards and
failure to cool down again. Then again I have met occasional EDS users who
only cool the detector when they need to use it! They do fine.

Ron Vane
XEI Scientific

-----Original Message-----
} From: James M. Ehrman {jehrman-at-mta.ca}
To: Microscopy Listserv {Microscopy-at-sparc5.microscopy.com}



From daemon Tue May 8 03:11:43 2001



From: Lifeng Dong :      lifengd-at-pdx.edu
Date: Tue, 08 May 2001 01:03:55 -0700 (PDT)
Subject: TEM2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am a graduate student of Portland State University.
I will take part in the Microscopy & Microanalysis,
2001 from August 5 to August 9. I will reserve room
from the Westin Long Beach and want to share it with
another male candidate.Please let me know as soon as
possible once you are intrested in it.

You can get the information about Westin Long Beach
from
http://www.pkghlrss.com/liveres/res.asp

Base rate $139 (Nightly rates may vary.)


Sincerely,

Lifeng



Lifeng Dong
Physics Department
Portland State University
P.O.Box 751
Portland,OR,97207-0751
U.S.A
Tel:503-725-8061
Fax:503-725-3888


From daemon Tue May 8 05:53:35 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 08 May 2001 06:50:37 -0400
Subject: Re: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html} {head} {/head} {body} Earl, {br}
He's trying to have a life!  The correct answer lies behind door #2.  Just
keep the dewar cold and the electronics off.  Your vacuum system status is
optional, depending upon the system and how well it protects itself. {br}
Ken Converse {br}
owner {br}
Quality Images {br}
third party SEM service {br}
Delta, PA {br}
{br}
Earl Weltmer wrote: {br}
{blockquote type="cite" cite="mid:000701c0d73c$90dbafe0$428ccd3f-at-pacbell.net"} {pre wrap=""} ------------------------------------------------------------------------ {br} The Microscopy ListServer -- Sponsor: The Microscopy Society of America {br} To Subscribe/Unsubscribe -- Send Email to {a class="moz-txt-link-abbreviated" href="mailto:ListServer-at-MSA.Microscopy.Com"} ListServer-at-MSA.Microscopy.Com {/a} {br} On-Line Help {a class="moz-txt-link-freetext" href="http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html"} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {/a} {br} -----------------------------------------------------------------------. {br} {br} {br} Stay home is probably your safest bet. {br} {br} Earl {br} {br} ----- Original Message ----- {br} {/pre}
{blockquote type="cite"} {pre wrap=""} From: "James M. Ehrman" {a class="moz-txt-link-rfc2396E" href="mailto:jehrman-at-mta.ca"} <jehrman-at-mta.ca> {/a} {br} {/pre} {/blockquote}
{pre wrap=""} {!----} To: "Microscopy Listserv" {a class="moz-txt-link-rfc2396E" href="mailto:Microscopy-at-sparc5.microscopy.com"} <Microscopy-at-sparc5.microscopy.com> {/a} {br} Sent: Monday, May 07, 2001 5:15 AM {br} Subject: what to do with unattended EDS on SEM {br} {br} {br} {/pre}
{blockquote type="cite"} {pre wrap=""} ------------------------------------------------------------------------ {br} The Microscopy ListServer -- Sponsor: The Microscopy Society of America {br} To Subscribe/Unsubscribe -- Send Email to {a class="moz-txt-link-abbreviated" href="mailto:ListServer-at-MSA.Microscopy.Com"} ListServer-at-MSA.Microscopy.Com {/a} {br} On-Line Help {a class="moz-txt-link-freetext" href="http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html"} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {/a} {br} -----------------------------------------------------------------------. {br} {br} {br} Hi Listers, {br} {br} I need to be away for two weeks, and would like opinions {br} on what is best to do with an EDS detector, LN2-wise, while {br} away. My options appear to be: {br} {br} 1. Leave everything on as normal. Don't really want to do this - I have {br} someone I trust to keep the LN2 dewar full, but not really handle the {br} microscope if something like a power failure or worse occurs. {br} {br} 2. Leave scope off, keep LN2 in EDS, hope residual vacuum in SEM {br} is adequate for two weeks. I'm guessing it's best to power off the {br} detector and computer, regardless. {br} {br} 3. Power down EDS, running detector through it's conditioning cycle, {br} leave everything off and at room temperature. {br} {br} 4. Stay home, chained to the system. {br} {br} Thoughts, experiences greatly appreciated. {br} {br} Cheers, {br} {br} Jim {br} {br} -- {br} {br} James M. Ehrman {br} Digital Microscopy Facility {br} Mount Allison University {br} Sackville, NB E4L 1G7 {br} CANADA {br} {br} phone: 506-364-2519 {br} fax: 506-364-2505 {br} email: {a class="moz-txt-link-abbreviated" href="mailto:jehrman-at-mta.ca"} jehrman-at-mta.ca {/a} {br} www: {a class="moz-txt-link-freetext" href="http://www.mta.ca/~jehrman"} http://www.mta.ca/~jehrman {/a} {br} {br} {br} {br} {/pre} {/blockquote}
{/blockquote}
{br}
{/body} {/html}



From daemon Tue May 8 07:52:54 2001



From: kfield-at-fast.net
Date: Tue, 8 May 2001 07:49:06 -0500
Subject: Ask-A-Microscopist :illuminator for an old Nikon Stereo microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(kfield-at-fast.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, May
7, 2001 at 21:49:02
---------------------------------------------------------------------------

Email: kfield-at-fast.net
Name: Alex Field

Organization: Keith Valley Middle School

Education: 6-8th Grade Middle School

Location: Horsham, PA USA

Question: I am looking for a substage light or illuminator for an old
Nikon Stereo microscope I was given. I want to be able to look at
water samples from ponds at the organisms in the ponds near my home.
Can you tell me where I can buy one?

Thank you,

Alex

---------------------------------------------------------------------------


From daemon Tue May 8 09:39:49 2001



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 8 May 2001 10:33:34 -0400 (EDT)
Subject: Illuminator for Olympus POS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone happen to have an extra illuminator for an Olympus POS??

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)


From daemon Tue May 8 10:24:30 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 8 May 2001 09:11:13 -0600
Subject: Prof. Dr. L. Reimer died on 29. April 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very sad, indeed.

As one of his students I feel a professional as well as a personal loss. I
remember him as a great teacher, a good advisor, and I also recall many a
bottle of Champaign when one of his students passed a final exam.

He will be missed...

Mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Petra Wahlbring [mailto:petra.wahlbring-at-nexgo.de]
Sent: Monday, May 07, 2001 12:03 AM
To: Microscopy-at-sparc5.microscopy.com


Dear Colleagues,

I think some of you will have an interest in the following, very sad news:

Prof. Dr. Ludwig Reimer (Muenster, Germany) died on April 29th, 2001.

I don't know how to express this correctly in english, but I feel it as a
very big loss. I will leave it to others to review his scientific
contributions to the community, I know him best as teacher to his students
to whom he was always devoted.

Just wanted to let you know...

Petra Wahlbring
-------------------------------------

Dr. Petra Wahlbring
Centre de Recherche Gabriel Lippmann
Laboratoire d'Analyse des Matériaux
162a, avenue de la Faiencerie
L-1511 Luxembourg
petra.wahlbring-at-crpgl.lu



From daemon Tue May 8 11:58:31 2001



From: Jill Verlander Reed :      verlaj-at-medicine.ufl.edu
Date: Tue, 8 May 2001 12:46:35 -0500
Subject: TEM placenta perfusion/fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working with an investigator who has been doing perfusion/fixation
of rat placentas for EM immunolocalization. He is dissatisfied with the
morphologic preservation and believes the problem is related to the
mechanics of the perfusion.

Does anyone out there have experience doing these perfusions or know of
someone that is that we could contact to get some advice on how to
optimize the fixation procedure?

Thanks in advance for any help that you can offer.

Sincerely,


Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299


From daemon Tue May 8 12:34:31 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 8 May 2001 11:22:23 -0600
Subject: RE: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken,

could you explain the rationale behind your suggestion?

I thought, the detector has to be kept cold for 2 reasons:

a) to keep the preamplifiers and detector cold for better S/N ratio
b) to keep the ions (Li) in the crystal from diffusing in the electric field


If, and only if, this is correct, there is no need to keep the detector cold
if the electronics and high voltage power supply are turned off. In fact, if
you keep everything cold, it could attract dirt over time (cryo-trap),
degrading the performance.

I agree with you on the vacuum, though. If you can, keep the scope under
vacuum. If not, you may have to pump for a while to get out moisture, or
perhaps even bake it out.

I could be wrong -- has happened before.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: {mailto:info-at-soft-imaging.com}
web: {http://www.soft-imaging.com/}
===================================
-----Original Message-----
From: Ken Converse [mailto:qualityimages-at-netrax.net]
Sent: Tuesday, May 08, 2001 4:51 AM
To: Earl Weltmer; MSA, listserver
Subject: Re: what to do with unattended EDS on SEM


------------------------------------------------------------------------ The
Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line
Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.
Earl,
He's trying to have a life! The correct answer lies behind door #2.
Just keep the dewar cold and the electronics off. Your vacuum system status
is optional, depending upon the system and how well it protects itself.
Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Earl Weltmer wrote:

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
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-----------------------------------------------------------------------.


Stay home is probably your safest bet.

Earl

----- Original Message -----
From: "James M. Ehrman" {jehrman-at-mta.ca}
{mailto:jehrman-at-mta.ca}
To: "Microscopy Listserv" {Microscopy-at-sparc5.microscopy.com}
{mailto:Microscopy-at-sparc5.microscopy.com}
Sent: Monday, May 07, 2001 5:15 AM
Subject: what to do with unattended EDS on SEM



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy
Society of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
On-Line Help
{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}

-----------------------------------------------------------------------.


Hi Listers,

I need to be away for two weeks, and would like
opinions
on what is best to do with an EDS detector,
LN2-wise, while
away. My options appear to be:

1. Leave everything on as normal. Don't really want
to do this - I have
someone I trust to keep the LN2 dewar full, but
not really handle the
microscope if something like a power f!
ailure or worse occurs.

2. Leave scope off, keep LN2 in EDS, hope residual
vacuum in SEM
is adequate for two weeks. I'm guessing it's
best to power off the
detector and computer, regardless.

3. Power down EDS, running detector through it's
conditioning cycle,
leave everything off and at room temperature.

4. Stay home, chained to the system.

Thoughts, experiences greatly appreciated.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca {mailto:jehrman-at-mta.ca}
www: {http://www.mta.ca/~jehrman}



From daemon Tue May 8 14:17:20 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 08 May 2001 11:58:46 -0700
Subject: RE: glycol methacrylate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listserver,
Glycol methyacrylate can be cut easily with glass knives at 1 or 2 microns. The sections are like very fine cellophane and wrinkles can be spread out by placing them on a fairly deep(3-4inches) water bath...no heat. Then, they can be picked up using a paint brush onto clean glass slides and dried and stained.

Barbara L. Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster St.
San Francisco, CA
email: Bplowman-at-sf.uop.edu
Ph: 415-929-6692



From daemon Tue May 8 14:51:10 2001



From: Lifeng Dong :      lifengd-at-pdx.edu
Date: Tue, 08 May 2001 12:45:24 -0700 (PDT)
Subject: TEM2001-Longbeach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

Sorry. The website in the last e-mail is not very exact.In fact,you can get
the information about Westin Long Beach directly from

www.pkghlrss.com/events/mm2001/mm2001.html

I am a graduate student of Portland State University. I will take part in
the Microscopy & Microanalysis, 2001 from August 5 to August 9. I will
reserve a room from the Westin Long Beach and want to share it with another
male candidate.Please let me know as soon as possible once you are intrested in
it.

Base rate $139 (Nightly rates may vary.)

Thanks,

Lifeng

Lifeng Dong
Physics Department
Portland State University
P.O.Box 751
Portland,OR,97207-0751
U.S.A
Tel:503-725-8061
Fax:503-725-3888


From daemon Tue May 8 16:40:50 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Tue, 8 May 2001 17:43:06 -0400
Subject: image contrast calculation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

we work on polymers containing a small fraction of carboxylic or sulfonic
acids or the respective metal carboxylates and sulfonates. We currently try
to map these different metal ions used to neutralize these acid groups by
means of EFTEM or x-ray mapping. The metal ions are thought to cluster and
form small "aggregates" within a pretty homogeneous polymer matrix.
Recently, it was suggested to calculate the contrast between the polymer
matrix (a C and O or C, S, and O containg material) and the metal ions in
order to ensure that we are not wasting our time trying something
impossible. Now: HOW do I calculate the contrast between a metal ion and a
polymer matrix ? References and procedures are very welcome !

Thanks in Advance

Andreas

*************************************************
Dr. Andreas Taubert
Dept. of Materials Science and Engineering
3231 Walnut Street
University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************




From daemon Tue May 8 17:49:41 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 08 May 2001 18:46:45 -0400
Subject: Re: what to do with unattended EDS on SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html} {head} {/head} {body} Mike, one of the other replies to this answered
the question quite well.  What happens when you let it warm up is the zeolites
in the dewar (this is actually a sorption pump) release what they have captured. 
This may then condense on the SiLi crystal, degrading resolution and sensitivity. 
If enough stuff has been captured by the zeolites, due to a leaking system,
you will end up with more than 1 atmosphere in the dewar and blow your window
outwards. {br}
{br}
When Kevex came out with their "super dry" detector, keeping it cold was
not necessary, but if you turned the ION pump off, your warranty was null
and void.  Clean is the key and if you have a dewar with zeolites, that means
cold, except to warm it up only enough to remove accumulated ice and then
immediately cool it again.  The manufacturers ship warm because the dewar
was just pumped out and leak-tested, but my understanding is that you should
not have it warm for more than a month total. {br}
{br}
Ken Converse {br}
owner {br}
Quality Images {br}
third party SEM service {br}
Delta, PA {br}
{br}
Mike Bode wrote: {br}
{blockquote type="cite" cite="mid:D1B635C6092DD411B0700020781025DE0F6128-at-lakewood.soft-imaging.com"}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} Ken, {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} could
you explain the rationale behind your suggestion? {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} I
thought, the detector has to be kept cold for 2 reasons: {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} a) to
keep the preamplifiers and detector cold for better S/N
ratio {/span} {/font} {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} b) to
keep the ions (Li) in the crystal from diffusing in the electric field
{/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} If,
and only if, this is correct, there is no need to keep the detector cold if the
electronics and high voltage power supply are turned off. In fact, if you keep
everything cold, it could attract dirt over time (cryo-trap), degrading the
performance. {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} I
agree with you on the vacuum, though. If you can, keep the scope under vacuum.
If not, you may have to pump for a while to get out moisture, or perhaps even
bake it out. {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} I
could be wrong -- has happened before. {/span} {/font} {/div}
{div}   {/div}
{div} {font face="Arial" color="#0000ff" size="2"} {span class="056345816-08052001"} mike {/span} {/font} {/div}
{div}   {/div}
{p} {font face="Arial" color="#000000" size="2"} Michael Bode, Ph.D. {/font} {br} {font face="Arial" color="#000000" size="2"} Soft Imaging System Corp. {/font} {br} {font face="Arial" color="#000000" size="2"} 1675 Carr St., #105N {/font} {br} {font face="Arial" color="#000000" size="2"} Lakewood, CO 80215 {/font} {br} {font face="Arial" color="#000000" size="2"} =================================== {/font} {br} {font face="Arial" color="#000000" size="2"} phone:  (888) FIND SIS {/font} {br} {font face="Arial" color="#000000" size="2"}         (303)
234-9270 {/font} {br} {font face="Arial" color="#000000" size="2"} fax:   
(303) 234-9271 {/font} {br} {font face="Arial" color="#000000" size="2"} email:  {a target="_blank" href="mailto:info-at-soft-imaging.com"} mailto:info-at-soft-imaging.com {/a} {/font} {br} {font face="Arial" color="#000000" size="2"} web:    {a target="_blank" href="http://www.soft-imaging.com/"} http://www.soft-imaging.com {/a} {/font} {br} {font face="Arial" color="#000000" size="2"} =================================== {/font} {/p}
{blockquote} {div class="OutlookMessageHeader" dir="Ltr" align="Left"} {font face="Tahoma" size="2"} -----Original Message----- {br} {b} From: {/b} Ken Converse
[ {a class="moz-txt-link-freetext" href="mailto:qualityimages-at-netrax.net"} mailto:qualityimages-at-netrax.net {/a} ] {br} {b} Sent: {/b} Tuesday, May 08, 2001 4:51
AM {br} {b} To: {/b} Earl Weltmer; MSA, listserver {br} {b} Subject: {/b} Re: what to
do with unattended EDS on
SEM {br} {br} {/font} {/div} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- Send Email to {a class="moz-txt-link-abbreviated" href="mailto:ListServer-at-MSA.Microscopy.Com"} ListServer-at-MSA.Microscopy.Com {/a} On-Line
Help {a class="moz-txt-link-freetext" href="http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html"} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {/a}
-----------------------------------------------------------------------.
Earl, {br} He's trying to have a life!  The correct answer lies behind door
#2.  Just keep the dewar cold and the electronics off.  Your vacuum
system status is optional, depending upon the system and how well it protects
itself. {br} Ken Converse {br} owner {br} Quality Images {br} third party SEM
service {br} Delta, PA {br} {br} Earl Weltmer wrote: {br} {blockquote cite="mid:000701c0d73c$90dbafe0$428ccd3f-at-pacbell.net" type="cite"} {pre wrap=""} ------------------------------------------------------------------------ {br} The Microscopy ListServer -- Sponsor: The Microscopy Society of America {br} To Subscribe/Unsubscribe -- Send Email to {a class="moz-txt-link-abbreviated" href="mailto:ListServer-at-MSA.Microscopy.Com"} ListServer-at-MSA.Microscopy.Com {/a} {br} On-Line Help {a class="moz-txt-link-freetext" href="http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html"} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {/a} {br} -----------------------------------------------------------------------. {br} {br} {br} Stay home is probably your safest bet. {br} {br} Earl {br} {br} ----- Original Message ----- {br} {/pre} {blockquote type="cite"} {pre wrap=""} From: "James M. Ehrman" {a class="moz-txt-link-rfc2396E" href="mailto:jehrman-at-mta.ca"} <jehrman-at-mta.ca> {/a} {br} {/pre} {/blockquote} {pre wrap=""} {!----} To: "Microscopy Listserv" {a class="moz-txt-link-rfc2396E" href="mailto:Microscopy-at-sparc5.microscopy.com"} <Microscopy-at-sparc5.microscopy.com> {/a} {br} Sent: Monday, May 07, 2001 5:15 AM {br} Subject: what to do with unattended EDS on SEM {br} {br} {br} {/pre} {blockquote type="cite"} {pre wrap=""} ------------------------------------------------------------------------ {br} The Microscopy ListServer -- Sponsor: The Microscopy Society of America {br} To Subscribe/Unsubscribe -- Send Email to {a class="moz-txt-link-abbreviated" href="mailto:ListServer-at-MSA.Microscopy.Com"} ListServer-at-MSA.Microscopy.Com {/a} {br} On-Line Help {a class="moz-txt-link-freetext" href="http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html"} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html {/a} {br} -----------------------------------------------------------------------. {br} {br} {br} Hi Listers, {br} {br} I need to be away for two weeks, and would like opinions {br} on what is best to do with an EDS detector, LN2-wise, while {br} away. My options appear to be: {br} {br} 1. Leave everything on as normal. Don't really want to do this - I have {br} someone I trust to keep the LN2 dewar full, but not really handle the {br} microscope if something like a power f! {br} ailure or worse occurs. {br} {br} 2. Leave scope off, keep LN2 in EDS, hope residual vacuum in SEM {br} is adequate for two weeks. I'm guessing it's best to power off the {br} detector and computer, regardless. {br} {br} 3. Power down EDS, running detector through it's conditioning cycle, {br} leave everything off and at room temperature. {br} {br} 4. Stay home, chained to the system. {br} {br} Thoughts, experiences greatly appreciated. {br} {br} Cheers, {br} {br} Jim {br} {br} -- {br} {br} James M. Ehrman {br} Digital Microscopy Facility {br} Mount Allison University {br} Sackville, NB E4L 1G7 {br} CANADA {br} {br} phone: 506-364-2519 {br} fax: 506-364-2505 {br} email: {a class="moz-txt-link-abbreviated" href="mailto:jehrman-at-mta.ca"} jehrman-at-mta.ca {/a} {br} www: {a class="moz-txt-link-freetext" href="http://www.mta.ca/~jehrman"} http://www.mta.ca/~jehrman {/a} {br} {br} {br} {br} {/pre} {/blockquote} {/blockquote} {br} {/blockquote}
{/blockquote}
{br}
{/body} {/html}



From daemon Tue May 8 18:24:11 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Tue, 8 May 2001 19:18:30 -0400
Subject: Thank You/Summary: Lens Help

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What a great venue! Is this list wonderful, or what?

Thank you, all, for the replies on lenses. I have kind of wondered
about the markings for some time, but now I needed to understand
what we have on the scope, and in the drawer.

To sum it up:
Epi: Illumination can be supplied from above, through the
objective.

Plan: Objective has a very flat field of focus.

Neofluar: Fluorite components for better spherical and
chromatic corrections.

44 23 80: Zeiss catalog number for the lens.

MDPlan: Identified as "Metallurgical" & "Darkfield", but there
is no darkfield light path in the objective. The
Olympus
web site lists the MDPlan as a brightfield
objective,
but does not indicate what "MD" stands for.

Yes, it really is a 150x/0.95 lens. We also have a 160x/0.95
Leitz Wetzlar lens that I did not mention.

(infinity sign)/0: Infinity corrected lens/no coverslip.

f=180: Tube length of the microscope in mm.

IR: Lenses are ground and coated to optimize for infrared.

Two fantastic web sites were identified which really do answer
more questions than were ever answered on any episode of
"Soap".

www.micro.magnet.fsu.edu/primer/anatomy/specifications.html
or www.micro.magnet.fsu.edu/primer/anatomy/index.html

www.microscopyu.com/articles/optics/objectivespecs.html
or www.microscopyu.com

No, I did not think it was mundane, either. I just wanted to give
warning to those that seem to be on a higher plane than me.
I have an insatiable curiosity that has provided for me through
the last 17 years of integrated circuit failure analysis.

Regards,
Darrell



From daemon Tue May 8 18:26:20 2001



From: jms-at-vetmed.wsu.edu ()
Date: Tue, 8 May 2001 18:24:26 -0500
Subject: Ask-A-Microscopist: fluorscent tracers and labeled antibodies to

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Below is the result of your feedback form. It was submitted by
(jms-at-vetmed.wsu.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
8, 2001 at 18:14:38
---------------------------------------------------------------------------

Email: jms-at-vetmed.wsu.edu
Name: Janice Siegford

Organization: Washington State University

Education: Graduate College

Location: Pullma, WA, USA

Question: I am a neuroscience grad student at WSU, interested in
motor neurons in the spinal cord. I use different fluorscent tracers
and labeled antibodies to visualize cells and proteins of interest
through the microscope (we use a Leitz Diaplan). I am looking for
information on cameras that are sensitive enough to visualize the
fluorescence and transmit the images to my computer for image
analysis (by optimas). What camera makes and models do you recommend?
I use wide band UV, narrow band blue, and narrow band green filters
most often. Thanks very much for any help you can give me!

---------------------------------------------------------------------------


From daemon Tue May 8 23:09:16 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 9 May 2001 15:36:51 GMT+1200
Subject: RE: what to do with unattended EDS on SEM

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}
} I agree with you on the vacuum, though. If you can, keep the scope
} under vacuum. If not, you may have to pump for a while to get out
} moisture, or perhaps even bake it out.
}

Bake it out?

Really?

Does anyone really do this/has anyone ever actually done it?

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed May 9 03:15:20 2001



From: ken blight :      blight-at-icrf.icnet.uk
Date: Wed, 9 May 2001 09:09:25 +0100
Subject: Vital stain

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--
Hi,
I am looking for a stain to use on unfixed cells and organelles
so that I can see them throughout processing for EM (especially for
cryo-sectioning). I believe such a stain is called a vital stain.
Any help on this matter will be greatly appreciated.
Many thanks
Ken Blight.
Senior Scientific Officer
Imperial Cancer Research Fund
London
England.


From daemon Wed May 9 08:18:39 2001



From: Dr. Klaus Jandt :      K.Jandt-at-bristol.ac.uk
Date: Wed, 9 May 2001 14:10:32 +0100
Subject: Vacancy Postdoctoral Research Assistant

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University of Bristol in co-operation with Glaxo SmithKline

Department of Oral and Dental Science, Biomaterials Group

The Biomaterials Science Group in the Department of Oral and Dental Science,
University of Bristol in collaboration with Glaxo SmithKline have a vacancy
for a Postdoctoral Research Assistant in the Biomaterials Science Group on
the project Interaction Mechanisms of Polymers at Interfaces of Mineralised
Tissues.

The research area involves the study of the physical and chemical properties
and interaction mechanisms of different polymers at interfaces of
mineralised tissues. You will have recently been awarded a PhD in an
appropriate field and will ideally have experience in scanning probe
microscopy (AFM) of biological materials and other analytical techniques and
an interest in medical research. You will work in Dr. Jandts group and
interact with scientists at Glaxo Smith Kline.

The University of Bristol is one of the leading research universities in the
UK and provides an outstanding scientific training environment to enhance
your qualification. Bristol is located in the attractive south-west of
England. The group is involved in exciting, interdisciplinary projects and
maintains appropriate state of the art instrumentation. There exist
opportunities for additional interactions with clinical scientists and other
centres at the university.

We are looking for a dynamic and exceptionally well-qualified postdoctoral
researcher who can interact effectively in an international and
interdisciplinary team. The appointment will be on a Research Assistant 1A
scale with a salary range of # 16775 to # 20465. This is a full time
appointment and initially for one year.

Applicants should include a short CV, stating research experience and
interests, publication list and addresses of two referees. The review of
applications will start 24 May 2001 and will continue until the post has
been filled.

For further details telephone ++44 117 954 6947, minicom ++ 44 117 928 8894
or E-Mail Recruitment-at-bris.ac.uk (stating postal address ONLY) quoting
reference 7401. Applicants wishing to apply electronically must complete an
"APPLICATION FORM FOR AN ACADEMIC VACANCY", found at
http://www.bris.ac.uk/Depts/Personnel/recruit.htm

Closing Date May 24th, 2001

-----------------------------------------------------------------
Dr. rer. nat. Klaus D. Jandt
Senior Lecturer in Dental Materials Science and Biomaterials
University of Bristol, Department of Oral and Dental Science
Lower Maudlin Street, Bristol, BS1 2LY, UK
Phone: +44-117-9284418, Fax: ++44-117-9284780
Internet: K.Jandt-at-bris.ac.uk
WWW: http://www.dent.bris.ac.uk/Biomaterials/kdj.htm
"We make Biomaterials Science work!"
Imua a lanakila



From daemon Wed May 9 13:36:21 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Wed, 9 May 2001 14:34:53 -0400
Subject: Amray 1200 spare parts

Contents Retrieved from Microscopy Listserver Archives
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I have 2 boxes of 10 each retipped tungsten filaments for an Amray 1200 up
for grabs for the cost of shipping (our old SEM was destroyed in a plant
explosion -- well, actually, a pressure vessel failure, but same end
result.) Also included are column liner cleaning tools and a few bulbs.
Please contact me directly if you're interested.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
jlagoy-at-bodycote-imt.com



From daemon Wed May 9 14:29:25 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 09 May 2001 15:17:40 -0400
Subject: RMC?

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Hi Listers,

Is RMC still around? I have a RMC MT-7000 ultramicrotome that needs a tune up or something (it chatters during the cutting stroke). Does anybody have the number for whoever is taking care of RMC products now. Ventana?

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Wed May 9 18:19:35 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Wed, 9 May 2001 18:15:43 -0500
Subject: TEM 120 film

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Colleagues, does anyone know of a similar film (Fuji ?) for Kodak Technical
Pan Film 120? Ours has been on backorder close to a month and now we are
being informed that not until tomorrow, maybe, may film arrive. Our TP120
is being used for the Zeiss EM 109. Many thanks for any responses Teresa


From daemon Wed May 9 18:19:35 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 9 May 2001 18:16:25 -0500
Subject: Re: TEM 120 film

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"Flores, Teresa" wrote:

} Colleagues, does anyone know of a similar film (Fuji ?) for Kodak Technical
} Pan Film 120? Ours has been on backorder close to a month and now we are
} being informed that not until tomorrow, maybe, may film arrive. Our TP120
} is being used for the Zeiss EM 109. Many thanks for any responses Teresa

Rather than find another film/developer combination (with experimentation
for correct exposure, aggravation, etc.), try another vendor for Tech Pan. Any
professional camera store should have at least a dozen rolls in stock. B&H
Photo and Video in New York City and Calumet in Chicago come to mind. They have
800 numbers for ordering and can ship overnight if you are in a real bind. Even
if it is not in stock they can have Kodak ship a 'brick' (20 rolls I think)
directly to you.
Kodak TMax 100 is a fine-grain B&W film that would be, I think, the closest
substititute. It does not have as much contrast as Tech Pan and I don't know
how well it works with whatever developer you are using. Call Kodak
(1-800-242-2424) and see what they recommend.
Finding an alternate source for Tech Pan would be simpler than
reconfiguring your exposure/film/development protocol.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************


From daemon Wed May 9 20:42:28 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 09 May 2001 18:40:47 -0700
Subject: Re: RMC?

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http://www.rmcproducts.com
(520) 745-0001
Boeckeler Inst. Inc.

Good Luck,
Sergey.

At 03:17 PM 5/9/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu May 10 07:44:58 2001



From: BNguyen260-at-aol.com
Date: Thu, 10 May 2001 08:33:42 EDT
Subject: Re: TEM 120 film

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--part1_12.ca28a65.282be4a6_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

We - Electron Microscopy Sciences- have no problem to supply you this Kodak
TP-120 film (#151-1054). Out catalog number for this is #74130 for 5 rolls
lot and #74133 for 20 rolls lot.
Phone: 800-523-5874. Web: www.emsdiasum.com

Bang Nguyen
EMS

--part1_12.ca28a65.282be4a6_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} We - Electron Microscopy Sciences- have no problem to supply you this Kodak
{BR} TP-120 film (#151-1054). Out catalog number for this is #74130 for 5 rolls
{BR} lot and #74133 for 20 rolls lot.
{BR} Phone: 800-523-5874. Web: www.emsdiasum.com
{BR}
{BR} Bang Nguyen
{BR} EMS {/FONT} {/HTML}

--part1_12.ca28a65.282be4a6_boundary--


From daemon Thu May 10 08:09:26 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Thu, 10 May 2001 08:02:52 -0500
Subject: Re: reference center for neuromuscular

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Vickie,we have an excellent reference center for neuromuscular biopsies
here at LSUHSC in New Orleans, LA (504) 599-0281 Cheryl Vega is the
technologist. Teresa
} Hello,
} I am looking for a good reference lab that deals with muscle tissue. At
} present we are using Athena Labs but are having horrendous tat. Do you use
} any lab out there for
} the more sophisticated tests done on frozen muscle? We do all the routine
} tests in house. Please let me know what labs are avaliable. Thanks in advance
} for your help.
} Vickie Hackett


From daemon Thu May 10 08:23:41 2001



From: jshields-at-cb.uga.edu
Date: Thu, 10 May 2001 09:19:02 -0400
Subject: boekeler and RMC

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RMC is currently being supported by Boekeler Instruments.
The guy to contact for service is Al Coritz, (800) 552-2262. He's a
great guy and will set you up.

John Shields
EM Lab
University of Georgia
jshields-at-cb.uga.edu
On 9 May 2001, at 15:17, Paula Sicurello wrote:

} --------------------------------------------------------------------
} -- -- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} -- -.
}
}
} Hi Listers,
}
} Is RMC still around? I have a RMC MT-7000 ultramicrotome that needs
} a tune up or something (it chatters during the cutting stroke).
} Does anybody have the number for whoever is taking care of RMC
} products now. Ventana?
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}



From daemon Thu May 10 09:58:24 2001



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Thu, 10 May 2001 10:48:32 -0400
Subject: RMC

Contents Retrieved from Microscopy Listserver Archives
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To the person looking for work to be done on a RMC ultramicrotome: Contact
Al Coritz at al-at-boeckeler.com Best of Luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu



From daemon Thu May 10 10:35:26 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 10 May 2001 11:27:10 -0400 (EDT)
Subject: Re: Vital stain

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Ken,

A vital stain is one that delineates living from dead cells. It
penetrates dead ones and leaves living (unfixed) ones unstained. It may
be that one of these (e.g., trypan blue) would work on your unfixed
cells as some of them would be dead, but it would not be staining the
cells of interest. You might be able to see them as a faint blue
pellet because of the dead ones.

As for what stain would go inside living cells and not alter
ultrastructure, I don't know. Sorry.


Sara Miller

Usually unstained cells look a little creamy, and you can see them in
frozen preparations.

On Wed, 9 May 2001, ken blight wrote:

} Date: Wed, 9 May 2001 09:09:25 +0100
} From: ken blight {blight-at-icrf.icnet.uk}
} To: microscorpy hints and tips {microscopy-at-sparc5.microscopy.com}
} Subject: Vital stain
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} --
} Hi,
} I am looking for a stain to use on unfixed cells and organelles
} so that I can see them throughout processing for EM (especially for
} cryo-sectioning). I believe such a stain is called a vital stain.
} Any help on this matter will be greatly appreciated.
} Many thanks
} Ken Blight.
} Senior Scientific Officer
} Imperial Cancer Research Fund
} London
} England.
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu May 10 11:35:16 2001



From: David Chiluiza :      david.chiluiza-at-usm.edu
Date: Thu, 10 May 2001 11:35:31 -0500
Subject: SEM fixation

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Regards to everyone.

I would like to thanks to all the people that have helped me with advises
about larval fixation for SEM.

I have a question, a person told me that he has obtained good results fixing
fish larvae with Karnovsky’s solution (a mixture of 8% paraformaldehyde,
0.2M sodium cacodylate buffer and 25% glutaraldehyde), but no one else has
mentioned it.
Please, let me know what do you think about this solution. I will have just
one group of larvae this year and that will be all for my thesis (I cannot
miss them). Last year I fixed larvae for TEM with glutaraldehyde + osmium
tetroxide + uranyl acetate and I understand that this procedure obscure the
sample surface and difficult observations.

Thanks again for your comments
David Chiluiza


__________________________________________________
David Chiluiza
USM, Institute of Marine Sciences
Gulf Coast Research Laboratory
703 East Beach Drive, Ocean Springs, MS 39566-7000

Phone: (228) 872-4287, Fax: (228) 872-4204
Email: david.chiluiza-at-usm.edu
Homepage: http://www.ims.usm.edu/students/gchiludc.htm
__________________________________________________



From daemon Thu May 10 11:59:10 2001



From: STANSMAN-at-aol.com
Date: Thu, 10 May 2001 12:54:18 EDT
Subject: Re: Ask-A-Microscopist:Questions on high quality photomicrographsusing a ster...

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Try Biocompare.com for product reviews.



Regards,

Stan Schwartz
Manager Bioscience Dept.
Nikon Instruments Inc.
1300 Walt Whitman Rd.
Melville, NY 11747

631-547-8500
Have you visited www.microscopyU.com lately?


From daemon Thu May 10 14:17:56 2001



From: Lisa Wright :      wright_lb-at-mercer.edu
Date: Thu, 10 May 2001 15:10:55 -0400
Subject: LM shield for protection from UV

Contents Retrieved from Microscopy Listserver Archives
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Hi -
I have an old Nikon Epiphot microscope that does not have a shield to
protect the user from UV. Does anyone know of a spare parts company
that would sell something this old? The other alternative is to have our
shop fabricate one. We have been told that if we do that, it needs to be
made of orange plexiglas. Does it need to be orange for protection?
Thank you in advance for your help!

Lisa Wright
Mercer University School of Medicine
Division of Basic Medical Sciences, Research
1550 College Street
Macon, GA 31207




From daemon Thu May 10 14:44:16 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 10 May 2001 14:32:45 -0500
Subject: Re: LM shield for protection from UV

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I would be interested in the general comments from the microscopy
community about how necessary such shields are. My Zeiss Axiophot
didn't come with one, my Nikon Diaphot and Optiphot did and we use
them, and my Olympus IX-70's did but we don't use it.

Not all plexiglas will stop UV equally well but clearly it doesn't
have to be orange since they sell UV safety glasses for use DNA gels
on UV light boxes that are clear plastic. Tom



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri May 11 08:26:12 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Fri, 11 May 2001 09:20:54 -0400
Subject: Re: LM UV shield

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I have had a Nikon Optiphot serviced annually for the past 12 years, and no
one has ever mentioned the need for UV shielding. I'll be very interested
to hear opinions about its necessity, too.

Jane


From daemon Fri May 11 08:34:19 2001



From: PHOBOS11-at-aol.com
Date: Fri, 11 May 2001 09:30:11 EDT
Subject: RMC

Contents Retrieved from Microscopy Listserver Archives
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--part1_cd.67634d3.282d4363_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

Hi Paula & Everyone

RMC is part of Boeckeler Instruments in Tucson Arizona. Last summer Ventana
Medical Systems sold the product line to Boeckeler Instruments.Ventana's
main focus was ISH & IHC in hospital histology labs and did not fit with
their long term goals. We offer sales of new instruments as well as service
on the old. Please feel free to contact me off line or visit ourwebsite
listed below for further information.


Al Coritz
Sales & Service Manager
RMC Products
Office: 520-745-0001
Fax: 520-745-0004
Cell: 520-465-3598
Al-at-boeckeler.com
WWW.rmcproducts.com


--part1_cd.67634d3.282d4363_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} Hi Paula & Everyone
{BR}
{BR} RMC is part of Boeckeler Instruments in Tucson Arizona.  Last summer Ventana
{BR} Medical Systems sold the product line to Boeckeler Instruments.Ventana's
{BR} main focus was ISH & IHC in hospital histology labs and did not fit with
{BR} their long term goals.  We offer sales of new instruments as well as service
{BR} on the old.  Please feel free to contact me off line or visit ourwebsite
{BR} listed below for further information.
{BR}
{BR}
{BR} Al Coritz
{BR} Sales & Service Manager
{BR} RMC Products
{BR} Office: 520-745-0001
{BR} Fax: 520-745-0004
{BR} Cell: 520-465-3598
{BR} Al-at-boeckeler.com
{BR} WWW.rmcproducts.com
{BR} {/FONT} {/HTML}

--part1_cd.67634d3.282d4363_boundary--


From daemon Fri May 11 08:43:49 2001



From: Alexander Mironov :      mironov-at-dcbo.cmns.mnegri.it
Date: Fri, 11 May 2001 08:42:07 -0500
Subject: About digitalized video microscope

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Dear Listers,
I need your suggestions about the possiblity to buy a system based on
the inverted microscope, but suitable for deconvolution and
time-lapse analysis of fast events in living cells.

We would like to buy such system. Whinch one is the best (I mean
quality devided on price)?

Sincerely yours, Alexander Mironov
Consorzio Mario Negri Sud, Italy


From daemon Fri May 11 08:48:50 2001



From: Gary Lovell :      gllovel-at-ppco.com
Date: Fri, 11 May 2001 13:43:58 -0000
Subject: Cameca & JEOL Service Query

Contents Retrieved from Microscopy Listserver Archives
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We are evaluating the Cameca SX-100 and JEOL JXA-8200 electron microprobes
as a replacement for a 20 year old JEOL 733.

We would appreciate any candid observations ( based on direct experience )
of the service provided by these two vendors.

We would appreciate a quick response.


Thanks



From daemon Fri May 11 09:25:01 2001



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Fri, 11 May 2001 09:18:15 -0500
Subject: re: reference center for neuromuscular

Contents Retrieved from Microscopy Listserver Archives
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Hi Vickie,
Another excellent lab for muscle is located in Buffalo, New York. Their
number is 719-878-7513.
I would also be interested in the routine stains that you do in your lab
and start a dialogue with you regarding the neuromuscular lab
commonalities.
F.Maiers



From daemon Fri May 11 09:36:39 2001



From: Dr. Klaus Jandt :      K.Jandt-at-bristol.ac.uk
Date: Fri, 11 May 2001 15:31:37 +0100
Subject: Vacancy Postdoctoral Research Assistant

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University of Bristol in co-operation with Glaxo SmithKline

Department of Oral and Dental Science, Biomaterials Group

The Biomaterials Science Group in the Department of Oral and Dental Science,
University of Bristol in collaboration with Glaxo SmithKline have a vacancy
for a Postdoctoral Research Assistant in the Biomaterials Science Group on
the project Interaction Mechanisms of Polymers at Interfaces of Mineralised
Tissues.

The research area involves the study of the physical and chemical properties
and interaction mechanisms of different polymers at interfaces of
mineralised tissues. You will have recently been awarded a PhD in an
appropriate field and will ideally have experience in scanning probe
microscopy (AFM) of biological materials and other analytical techniques and
an interest in medical research. You will work in Dr. Jandts group and
interact with scientists at Glaxo Smith Kline.

The University of Bristol is one of the leading research universities in the
UK and provides an outstanding scientific training environment to enhance
your qualification. Bristol is located in the attractive south-west of
England. The group is involved in exciting, interdisciplinary projects and
maintains appropriate state of the art instrumentation. There exist
opportunities for additional interactions with clinical scientists and other
centres at the university.

We are looking for a dynamic and exceptionally well-qualified postdoctoral
researcher who can interact effectively in an international and
interdisciplinary team. The appointment will be on a Research Assistant 1A
scale with a salary range of # 16775 to # 20465. This is a full time
appointment and initially for one year.

Applicants should include a short CV, stating research experience and
interests, publication list and addresses of two referees. The review of
applications will start 24 May 2001 and will continue until the post has
been filled.

For further details telephone ++44 117 954 6947, minicom ++ 44 117 928 8894
or E-Mail Recruitment-at-bris.ac.uk (stating postal address ONLY) quoting
reference 7401. Applicants wishing to apply electronically must complete an
"APPLICATION FORM FOR AN ACADEMIC VACANCY", found at
http://www.bris.ac.uk/Depts/Personnel/recruit.htm

2001 Closing Date May 24th, 2001

-----------------------------------------------------------------
Dr. rer. nat. Klaus D. Jandt
Senior Lecturer (Associate Professor) in Biomaterials Science
University of Bristol, Department of Oral and Dental Science
Lower Maudlin Street, Bristol, BS1 2LY, UK
Phone: +44-117-9284418, Fax: ++44-117-9284780
Internet: K.Jandt-at-bris.ac.uk
"We make Biomaterials Science work!"



From daemon Fri May 11 12:27:59 2001



From: Kevin W. Eliceiri :      eliceiri-at-facstaff.wisc.edu
Date: Fri, 11 May 2001 12:20:10 -0500
Subject: Biophotonics Postdoctoral Opening

Contents Retrieved from Microscopy Listserver Archives
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Postdoctoral Position available: Biophotonics Instrumentation
At University of Wisconsin-Madison, in the Instrumentation Laboratory
of Dr. John White.

Postdoctoral Scientists are sought for the development of new optical
instrumentation with an emphasis on spectral/lifetime imaging.

Previous experience in one or more of the following areas will be a
primary consideration: optical design, non-linear optics, optical
system development, techniques such as optical trapping or laser
microsurgery. Experience with either confocal or multiphoton
laser-scanning microscopy would be beneficial as the central emphasis
of the study will involve these imaging techniques. Extensive
facilities for microscopy, electrical engineering, software design
and biological research are available for this project.

Ph.D.'s with multidisciplinary backgrounds from fields such as
applied physics, biomedical engineering, electrical engineering,
molecular/cell biology, nanotechnology or other related
interdisciplinary fields are encouraged to apply.

Position is available as soon as June 1, 2001 with the application
deadline open until the position is filled.

Please direct all questions via email to Kevin Eliceiri
(eliceiri-at-facstaff.wisc.edu)

See www.loci.wisc.edu/employment.html for more information and
application instructions.


--
Kevin W. Eliceiri

Lab Manager
Microscopy Research Laboratory
http://www.microscopy.wisc.edu

Project Director
Laboratory for Optical and Computational Instrumentation (LOCI)
http://www.loci.wisc.edu
271 Animal Sciences
1675 Observatory Dr.
Madison, WI 53706
608-263-6288 voice
608-262-4570 fax



From daemon Fri May 11 12:58:48 2001



From: Foran, David A :      DFORAN-at-ora.fda.gov
Date: Fri, 11 May 2001 13:53:14 -0400
Subject: digital camera & image collection

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We currently have a 6 year old Sony video camera & printer with PAX-IT image
capture software. We have had problems with the system over the years &
poor support because the components are from different manufacturers (we
bought it with an Olympus PLM & the computer was a hand me down from another
lab section) so the manufacturers like to blame each other rather than fix
the problem. We also found that the video camera gives very poor resolution
above about 100X. We like the PAX-IT user interface when it works.

We are going to ask for a bridge comparison microscope with bright field,
dark field, phase contrast, DIC, & simple pol. We also want to ask for a
digital camera, new computer, & new or upgraded image capture software.

Because our analytical work always has the potential to go to court, I
prefer software that does not allow the user to significantly alter the
image (overlays, labeling, etc. are alright) like our current version of
PAX-IT.

We will potentially use the camera & capture system on our other scopes
(Wild M3 stereo, Olympus BX50P, AO 1-10 Compound, & Nikon Labophot Phase
Contrast) & Polaroid MP-4 photo stand. (We currently use the video camera
with a macro zoom lens on the MP-4.)

Our samples are mostly 'filth' (insect fragments, hairs, molds, yeast) in
foods & some drugs.

Does anyone have any suggestion (positive or negative) about bridge
comparison microscope models/brands, digital cameras, & image capture
software?


David A. Foran, chemist
Food and Drug Administration
Kansas City District Lab
DFORAN-at-ORA.FDA.GOV
913-752-2163
913-752-2727 (voice mail)
913-752-2151 (fax)





From daemon Fri May 11 13:25:12 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 11 May 2001 21:20:30 +0100
Subject: LM shield for protection from UV

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Dear Colleagues,

Dr. Halcrow has kindly modified/corrected my earlier statement on vital
stains, but he was too polite to broadcast it to the net. I thought you
should benefit from his comments and am sending them on at the risk of my
embarassment. Anyway, my comments were based on what I was told many
moons ago in grad school. In our usage, the stain colored dead or dying
cells immediately, but was not taken up by viable cells, perhaps
because we stained and viewed immediatley without allowing active uptake
into the cells. From what I have read in the last few hours, it takes
several minutes (30-60) for many vital stains to be taken up by living
cells; also, cells (e.g., bacteria stained with janus green B) can go
back to colorless in 24 hr.

Based on this exchange, I looked up viral stains and offer the following:

Stedmann. Vital s: applied to cells or parts of cells while they are
still living.

Dorland. Vital s: staining of a tissue by a dye which is introduced
into a living organism and which, by virtue of affinity for certain
tissues, will stain those tissues (e.g., Conn: trypan blue injected IV
stains kidney tubules)

Conn. Examples: Trypan red, benzopurpurin 4B, brilliant purpurin R,
viral red, benzil orange, benzyl orange 2R, azo blue, dianil blue 2R,
brilliant congo blue RRW, trypan blue, Evans blue, vital new red.

Thompson. Bismark brown(s) Y, R, 53A, 53B, 53C; Janus green B; acridine
orange; acridine red.

HJ Conn's Biological Stains by RD Lillie
Selected Histochemical and Histopathological Methods by SW Thompson
Dorland and Stedman are medical dictionaries.


---probably more than you ever wanted to know about vital stains.


Sara





Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265

---------- Forwarded message ----------


It is a bad idea to expose your skin to the UV produced by a
mercury vapour lamp.
But, assuming the lamp housing itself is light-tight, UV shields are
only required if UV excitation is being used.
If you are working with FITC (blue), GFP (blue) or RITC (green) type
filters UV is not used for excitation, and the UV shield is
unnecessary.
Most pale yellow, yellow orange or red plastics absorb UV. Plexiglass
in these colours should be readily obtainable - sign makers may have
offcuts. If you cannot obtain yellow or orange plexiglass use an
acetate filter of the type used for stage lighting.
Chris

} ----- Original Message -----
} From: "Tom Phillips" {PhillipsT-at-missouri.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, May 10, 2001 8:32 PM
} Subject: Re: LM shield for protection from UV
}
}
}
} --------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
} ---.
} }
} }
} } I would be interested in the general comments from the microscopy
} } community about how necessary such shields are. My Zeiss Axiophot
} } didn't come with one, my Nikon Diaphot and Optiphot did and we use
} } them, and my Olympus IX-70's did but we don't use it.
} }
} } Not all plexiglas will stop UV equally well but clearly it doesn't
} } have to be orange since they sell UV safety glasses for use DNA
gels
} } on UV light boxes that are clear plastic. Tom
} }
} }
} }
} }
}
} ---------------------------------------------------------------------
} ---
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
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} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} ---------------------------------------------------------------------
} --.
} } }
} } }
} } } Hi -
} } } I have an old Nikon Epiphot microscope that does not have a
shield
} to
} } } protect the user from UV. Does anyone know of a spare parts
} company
} } } that would sell something this old? The other alternative is to
} have our
} } } shop fabricate one. We have been told that if we do that, it
needs
} to be
} } } made of orange plexiglas. Does it need to be orange for
protection?
} } } Thank you in advance for your help!
} } }
} } } Lisa Wright
} } } Mercer University School of Medicine
} } } Division of Basic Medical Sciences, Research
} } } 1550 College Street
} } } Macon, GA 31207
} }
} } --
} } Thomas E. Phillips, Ph.D.
} } Associate Professor of Biological Sciences
} } Director, Molecular Cytology Core Facility
} }
} } 3 Tucker Hall
} } Division of Biological Sciences
} } University of Missouri
} } Columbia, MO 65211-7400
} } (573)-882-4712 (voice)
} } (573)-882-0123 (fax)
} }
}



From daemon Fri May 11 16:42:34 2001



From: vem98-at-amrer.net
Date: Sat, 12 May 2001 07:35:05 -0600
Subject: Recieve Your Blessing 18210

Contents Retrieved from Microscopy Listserver Archives
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{HTML}
{BODY}
{P align=3Dcenter}
{FONT face=3D"MS Sans Serif"}
{FONT size=3D2}
{FONT color=3D"#000000"} Your Future! Your Choice! {/P} {BR}
{BR}
{/FONT}
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et Rich Quick Scheme" and feel they shouldn't even have to work to create =
any kind of wealth in their lives. You should know by now, this is simply =
not possible unless you win the lotto! You must be asking yourself "Well, =
what is possible then? If you have a strong desire and a good work ethic, =
you can change the situation you are in, and you can create wealth in your=
life!!! {BR}
What is your situation?..... {BR}
{BR}
{/FONT}
{FONT color=3D"#000000"} {UL} {LI} living from paycheck to paycheck {BR}
{LI} working for someone else who's wealth increases while you can't even p=
ay bills {BR}
{LI} in credit card debt to the point you can not even foresee ever paying =
them off {BR}
{LI} working so much you have no time to spend with your family {BR}
{LI} not able to put your kids through college {BR}
{LI} time to retire, and the only source of income you have is social secur=
ity {BR}
{LI} self-employed and it seems your business consumes your life {BR}
{/LI} {/LI} {/LI} {/LI} {/LI} {/LI} {/LI} {/UL} {/FONT}
{FONT color=3D"#000000"} {BR}
If you are in these situations, or one similar, and you are willing to rol=
l up your sleeves and make a change, then I invite you to read further: {B=
R}
{BR}
{P align=3Dcenter} {/FONT}
{FONT color=3D"#000000"} This is not MLM! {/P} {BR}
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{FONT color=3D"#000000"} I'm looking for the entrepreneurial minded, GOAL =
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{/BODY}
{/HTML}




From daemon Fri May 11 17:32:14 2001



From: Foran, David A :      DFORAN-at-ora.fda.gov
Date: Fri, 11 May 2001 18:27:45 -0400
Subject: digital watermarking

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Does any one know about the concept of digital watermarking for proving the
authenticity of an image?

David A. Foran, chemist
Food and Drug Administration
Kansas City District Lab
DFORAN-at-ORA.FDA.GOV
913-752-2163
913-752-2727 (voice mail)
913-752-2151 (fax)





From daemon Fri May 11 19:13:08 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 11 May 2001 17:11:21 -0700
Subject: Re: LM shield for protection from UV

Contents Retrieved from Microscopy Listserver Archives
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In general, real UV should not come though glass optics. In order to do so
the optics should be made from quartz like quartz cuvettes for
spectrophotometers. Unless the optic is not quartz there is no worry about
UV. Some "near-UV" (300 and above nm) light could come
through. Excitation wavelength for fluorescein is 490 nm (with emission at
520 nm) - far away from UV. Therefore I don't see any reason manufacturers
will design microscope to pass real UV. Technically it's very difficult
perform (and extremely expensive - you will easy know if your microscope is
equipped with quartz optics).

Sergey.

At 02:32 PM 5/10/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Sat May 12 00:38:04 2001



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Fri, 11 May 2001 22:31:12 -0700 (PDT)
Subject: Re: SEM fixation

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164 Ferne Court
Palo Alto, CA 94306
(650) 494-0472
[NelsonC51-at-excite.com]

May 11, 2001

Hi David ...
While I have never dealt with marine larvae of any kind (my M.A. thesis
project concerned some morphological aspects of ciliates), I have dealt with
Karnovsky's fixative. It's probably been suggested because the double effect
of formaldehyde and glutaraldehyde preserve certain features (most likely,
of the morphological type) better than either of those alone. However, that
fixative also has a high osmolarity, and I'm guessing that this fixative may
not work with marine animals.
Good luck with your thesis project.
Nelson Conti





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Sat May 12 20:38:15 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sat, 12 May 2001 18:45:58 -0700
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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Dear David

They do modify slightly some particular pixels in your picture. This
modification could not be recognizable by eye, but may be extracted using
special algorithm (as Adobe Photoshop Plug-In do it). This "mark" will
survive even after hard modification of the original picture, because it's
not an "image" it's more like special "pattern" spreaded across the
image. They claimed that even on very bad printed hardcopy that "water
mark" will survive as well as on cropped/changed images. Is it not
amusing! Watermark's "reader" is free, but you have to pay in order to be
able to "mark" your image.


Sergey

At 06:27 PM 5/11/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun May 13 02:51:00 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 13 May 2001 02:44:57 -0500
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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}
}
} Dear David
}
} They do modify slightly some particular pixels in your picture. This
} modification could not be recognizable by eye, but may be extracted
using
} special algorithm (as Adobe Photoshop Plug-In do it). This "mark" will
} survive even after hard modification of the original picture, because
it's
} not an "image" it's more like special "pattern" spreaded across the
} image. They claimed that even on very bad printed hardcopy that "water
} mark" will survive as well as on cropped/changed images. Is it not
} amusing! Watermark's "reader" is free, but you have to pay in order to
be
} able to "mark" your image.
}
}
Dear David,

I wold approach the field of digital forensics with extreme caution. In
the beginning I wold back up every image with a B&W shot of Color Slide
methods that have a substantial body of work backing them up. A slick
lawyer and painshop can morph you evidence into Porkies Hooter bar and
grill sign so fast is leaves you in the dust. And you will like the warm
unretouched glow that comes form film or slides.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Sun May 13 11:08:49 2001



From: Dr. Edgar Voelkl :      mm2002-at-ornl.gov
Date: Sun, 13 May 2001 11:59:19 -0400
Subject: M&M 2002 in Quebec

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Dear fellow microscopists,

Although it might seem early to talk about the Microscopy and
Microanalysis meeting in the year 2002 Quebec City, Canada, it is the
time where the symposia are established and the symposia organizers
determined.

In order to provide the best possible program, I, as program chair,
am soliciting suggestions for symposia for the year 2002. Your idea
for a great topic in any of the areas of "Physical Sciences,"
"Biological Sciences," or in "Advances in Instrumentation and
Techniques," would be highly appreciated; after all, it is our annual
meeting. Between now and June 30th 2001, I can be contacted under
the following address:

mm2002-at-ornl.gov

Please forward the request also to your colleagues.
With best regards,

Edgar Voelkl
Program Chair M&M 2002


P.S.:
For more information on the 2002 program, see:
http://msc.rsvs.ulaval.ca/2002/2002.html


From daemon Sun May 13 12:30:08 2001



From: bak-at-one.net ()
Date: Sun, 13 May 2001 12:28:18 -0500
Subject: Ask-A-Microscopist: pigment patterns in various colors of horse

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Below is the result of your feedback form. It was submitted by
(bak-at-one.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
May 12, 2001 at 18:58:42
---------------------------------------------------------------------------

Email: bak-at-one.net
Name: Barbara Kostelnik

Organization: Hippo-Logistics

Education: Graduate College

Location: Cincinnati, OH USA

Question: I want to see if there are different visible pigment
patterns in various colors of horse hairs as alleged by Dr. Ben Green
in his controversial book, The Color of Horses. Here are some
scanned images from his work:
http://www.hippo-logistics.com/equus/Dr.Green/images/buckskin.jpg
http://www.hippo-logistics.com/equus/Dr.Green/images/sorrel.jpg

One of our groups is using an old projecting microscope made by
Bioscope Manufacturing. I just ordered an even older version on the
internet to try myself. What equipment would YOU suggest to see if
these patterns exist? (So far it looks to us like they don't.)

Thank you very much!
Barbara Kostelnik
Hippo-Logistics.com

---------------------------------------------------------------------------


From daemon Sun May 13 12:30:08 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Sun, 13 May 2001 12:23:24 -0500
Subject: Re: glycol methacrylate

Contents Retrieved from Microscopy Listserver Archives
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Tom and Barbara,

I agree with Barbara that you can cut these blocks with as good a
result as in new blocks, but in my experience (specifically with
JB-4) the ease of cutting depends on the size of the block and how
dry it is. Too dry a block will cause chatter and too wet a block
will give you mushy looking sections. You can put the blocks in a
chamber with either a container of water or desiccant to change
this factor (don't touch either to the block). Also with JB-4, the
hydrated sections can only be "drawn" out of the water on the slide,
as they stick to anything they touch once their wet.

As for removing the material, I don't know of any successful method
for this on JB-4 blocks. Maybe it is possible on other types of
glycol methacrylate.

Karen Pawlowski, Ph.D.
UT Dallas,
Dallas, TX

Barbara Plowman wrote:
}
} ------------------------------------------------------------------------
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} Dear Listserver,
} Glycol methyacrylate can be cut easily with glass knives at 1 or 2 microns. The sections are like very fine cellophane and wrinkles can be spread out by placing them on a fairly deep(3-4inches) water bath...no heat. Then, they can be picked up using a paint brush onto clean glass slides and dried and stained.
}
} Barbara L. Plowman
} Univ. of the Pacific
} School of Dentistry
} 2155 Webster St.
} San Francisco, CA
} email: Bplowman-at-sf.uop.edu
} Ph: 415-929-6692


From daemon Sun May 13 12:37:23 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 13 May 2001 12:33:52 -0500
Subject: Re: digital watermarking

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Dear David,

I did not make the point I wanted in my last post about digital images in
court. Put your self on the stand and answer these questions. Sir, did
you modify the image in any way? Sir, is the image a true representation
of the object. Sir, does each pixel have the same response to light as
every other pixel? [three questions with mutually exclusive answers] Sir,
how can you prove to me that this image was not manipulated before you
watermarked it.

After you testimony the defenses expert explains the problem of having to
apply a LUT table to the image to get at better image because different
pixels have different gain and then gives a demo on image manipulation.

None of this comes up if you have a conventional silver print. If you need
to use image enhancement to show something you can show it in relation to
a negitive that can be examined by experts for modification. While digital
image can be examined for modifcation explaing it to a jury will be very
difficult to get them to understand after being shown how much an image
can be changed.

The day will come that digital images are defensible in court but I would
much rather be in the position of attacking one than defending one today.
With all the bad press coming out about the FBI lab and other forensic
labs I would be extremely careful to leave no opening for a crack in the
chain of evidence.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger






From daemon Sun May 13 12:57:12 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 13 May 2001 13:57:55 -0700
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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One thing that comes to mind is teaching new dogs old tricks.

As technology advances, the devices of 30, 50 and more years ago are
forgotten for the most part, however, for reasons of economy preservation or
nostalgia, some people like to keep, restore and maybe even use these
antiquated devices. It might be helpful and interesting to at least collect
information on the care and feeding of an old microscope, techniques of
micrography with a non-automatic camera and the best methods to follow when
trying to find and/or make substitutions for parts on aging equipment.

Ron L
----- Original Message -----
} From: "Dr. Edgar Voelkl" {mm2002-at-ornl.gov}
To: {Microscopy-at-sparc5.microscopy.com}
Cc: {3dem-at-sdsc.edu}
Sent: Sunday, May 13, 2001 11:59 AM


The most prominent digital watermarking company is Digimarc
http://www.digimarc.com

Digital watermarking is under the moniker "steganography."
It is quite interesting and definitely a challenge to accomplish
without disturbing the original (creating artifacts). Unfortunately,
Digimarc's method is not very resilient. It can be easily removed
using a relatively simple C program.

Advise that you tread lightly in this area until and unless new,
more robust technology becomes available.

gary g.


At 03:27 PM 5/11/2001, you wrote:

} Does any one know about the concept of digital watermarking for proving the
} authenticity of an image?
}
} David A. Foran, chemist
} Food and Drug Administration
} Kansas City District Lab
} DFORAN-at-ORA.FDA.GOV
} 913-752-2163
} 913-752-2727 (voice mail)
} 913-752-2151 (fax)



From daemon Sun May 13 17:59:00 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 13 May 2001 16:14:49 -0700
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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Dear Gordon

I am not advocate for "watermarking". Digital "watermarks" developed for
purpose to identify the "image creator" (who owned un-touched original
file). And the idea is that "watermark" may survive on hardly modified
image identified therefore the author. For instance, if somebody stole
image file from your WEB site and use it to create some poster and sell it,
you my prove that your image was used without authorization if "watermark"
exist. It's not my personal opinion, it how I understand the purpose of
"watermarking" from company who offered it (it's Adobe related, I believe).
As I mention in my original message, company claims that it's possible to
extract watermark from printed hard copy, poster in my example.

Nice negatives/slides are always a plus, but they are not protected from
fraud and fire. Watermarks is not about image quality, it's about some
security. You may keep your beautiful slides in achieve, but you must to
convert it in digital if you want to post it on Internet. Here watermarks
may work.

I don't see any reasons at all to use watermarks in forensics, but artists,
publishers may be interested.

Sergey

At 02:44 AM 5/13/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun May 13 21:06:59 2001



From: COURYHOUSE-at-aol.com
Date: Sun, 13 May 2001 21:55:19 EDT
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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--part1_bb.e72375e.28309507_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

Watermarks are great if you spend eons composing or editing a scanned image
and some hoser rips it off, puts it on his site and claims it is his!
the water mark allows you to quickly ID it.

Of course there are other ways to handle such scoundrels as well ... but that
will be left for another dissertation!

Ed Sharpe archivist for SMECC


} Subj: Re: digital watermarking
} Date: 5/13/01 6:48:14 PM US Mountain Standard Time
} From: sryazant-at-ucla.edu (Sergey Ryazantsev)
} To: gcouger-at-couger.com (Gordon Couger)
} CC: microscopy-at-sparc5.microscopy.com
}
}
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Gordon
}
} I am not advocate for "watermarking". Digital "watermarks" developed for
} purpose to identify the "image creator" (who owned un-touched original
} file). And the idea is that "watermark" may survive on hardly modified
} image identified therefore the author. For instance, if somebody stole
} image file from your WEB site and use it to create some poster and sell it,
} you my prove that your image was used without authorization if "watermark"
} exist. It's not my personal opinion, it how I understand the purpose of
} "watermarking" from company who offered it (it's Adobe related, I believe).
} As I mention in my original message, company claims that it's possible to
} extract watermark from printed hard copy, poster in my example.
}
} Nice negatives/slides are always a plus, but they are not protected from
} fraud and fire. Watermarks is not about image quality, it's about some
} security. You may keep your beautiful slides in achieve, but you must to
} convert it in digital if you want to post it on Internet. Here watermarks
} may work.
}
} I don't see any reasons at all to use watermarks in forensics, but artists,
} publishers may be interested.
}
} Sergey
}
} At 02:44 AM 5/13/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
}



--part1_bb.e72375e.28309507_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} Watermarks are great if you spend eons  composing  or editing a scanned image
{BR} and some hoser rips it off, puts it on his site and claims it is his!
{BR} the water mark allows you to quickly ID it.
{BR}
{BR} Of course there are other ways to handle such scoundrels as well ... but that
{BR} will be left for another dissertation!
{BR}
{BR} Ed Sharpe archivist for SMECC
{BR}
{BR}
{BR} {BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"} Subj: {B} Re: digital watermarking {/B}
{BR} Date: 5/13/01 6:48:14 PM US Mountain Standard Time
{BR} {I} From:    sryazant-at-ucla.edu (Sergey Ryazantsev)
{BR} To:    gcouger-at-couger.com (Gordon Couger)
{BR} CC:    microscopy-at-sparc5.microscopy.com
{BR} {/I}
{BR}
{BR}
{BR}
{BR} ------------------------------------------------------------------------
{BR} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} -----------------------------------------------------------------------.
{BR}
{BR}
{BR} Dear Gordon
{BR}
{BR} I am not advocate for "watermarking".  Digital "watermarks" developed for
{BR} purpose to identify the "image creator" (who owned un-touched original
{BR} file).  And the idea is that "watermark" may survive on hardly modified
{BR} image identified therefore the author. For instance, if somebody stole
{BR} image file from your WEB site and use it to create some poster and sell it,
{BR} you my prove that your image was used without authorization if "watermark"
{BR} exist.  It's not my personal opinion, it how I understand the purpose of
{BR} "watermarking" from company who offered it (it's Adobe related, I believe).
{BR} As I mention in my original message, company claims that it's possible to
{BR} extract watermark from printed hard copy, poster in my example.
{BR}
{BR} Nice negatives/slides are always a plus, but they are not protected from
{BR} fraud and fire.  Watermarks is not about image quality, it's about some
{BR} security.  You may keep your beautiful slides in achieve, but you must to
{BR} convert it in digital if you want to post it on Internet.  Here watermarks
{BR} may work.
{BR}
{BR} I don't see any reasons at all to use watermarks in forensics, but artists,
{BR} publishers may be interested.
{BR}
{BR} Sergey
{BR}
{BR} At 02:44 AM 5/13/01 -0500, you wrote:
{BR} >------------------------------------------------------------------------
{BR} >The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} >To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} >On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} >-----------------------------------------------------------------------.
{BR} >
{BR} >
{BR} >
{BR} {/BLOCKQUOTE}
{BR}
{BR} {/FONT} {/HTML}

--part1_bb.e72375e.28309507_boundary--


From daemon Mon May 14 01:45:23 2001



From: John Terlet :      john.terlet-at-adelaide.edu.au
Date: Mon, 14 May 2001 16:08:13 +0930
Subject: ACEM17, Adelaide, South Australia, Feb2002

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Just a Reminder

The 17th Australian Conference on Electron Microscopy will be held in
the Adelaide Convention Centre 4th - 8th February 2002, presenting you
with an opportunity to escape cold climes and enjoy the worlds best
fresh food and wines whilst participating in a conference with, the
majority of Australia's and some of the World's leading Electron
Microscopists, Scanned Probe Microscopists, and Confocal Microscopists.

The Australian Society for Electron Microscopy (Inc.) is the host
society for this meeting in Adelaide, which is the is the Capital of the
State of South Australia. Adelaide is a picturesque city and is the
business and educational centre for the state.
South Australia is noted for its glorious Mediterranean climate,
picturesque surfing beaches, aquaculture, unpolluted agriculture and
of course, 5 of the worlds truly great wine producing areas.

There are many unique and significant tourist destinations are within
easy reach of Adelaide. The Barossa Valley with its unique German
influence, Southern Vales and the Clare Valley are wine regions
producing Australia's most famous wines such as the Grange and Hill of
Grace. The rugged Flinders Ranges, Kangaroo Island, the Adelaide Hills
and Beaches, offer a variety of scenic and relaxing outings to take at
your convenience.

Planning for the meeting is well advanced and you will be receiving
registration of interest and calls for abstracts in the near future. We
have focused on the website as our major form of communication. I do
hope that you can visit the site from time to time to maintain an
awareness of the ACEM17 activities.
(http://www.adelaide.edu.au/CEMMSA/acem17)

The conference will incorporate a series of workshops covering a broad
range of topics. Student microscopists are encouraged to attend to
learn or to launch their presentation careers and
a series of student bursaries will be offered to facilitate this.
On behalf of the Organising Committee may I extend an invitation
to attend this important meeting.

--
John Terlet
Director
CEMMSA
(Centre for Electron Microscopy & MicroStructure Analysis)
Adelaide University
Ph: 61 8 8303 5855
Fax: 61 8 8303 4356
http://www.adelaide.edu.au/CEMMSA


From daemon Mon May 14 04:14:45 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 14 May 2001 04:08:44 -0500
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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Dear Sergey

In an earlier post there was a mention of the images being used in forenic
work. Then the watermark question. I sould have mentioned the forenic post
in my reply about watermarks.

My point being that I would be very nervous using a digital image in court
because of the ease that they can be manupulated and the fact that there
is no way to tell if it is orignal or not.

Gordon




From daemon Mon May 14 07:47:17 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 14 May 2001 09:22:43 -0400
Subject: Re: Fund transfer to cover dinners

Contents Retrieved from Microscopy Listserver Archives
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} Hong,

The check needs to be made payable to Penn State University not PUS.
Rosemary

} Rosemary, I talked to Stacie. She will send you a check for $2,400.00 next
} week. The check will be made payable to PUS. Is all this OK? Thank you.
}
} Hong
} ----------
} } From: Rosemary Walsh {rw9-at-psu.edu}
} } To: Hong Yi {hyi-at-emory.edu}
} } Subject: Fund transfer to cover dinners
} } Date: Fri, May 11, 2001, 11:24 AM
} }
}
} } Hi Hong,
} } After consulting with our admin. assistant, it would be best
} } to have Stacie send me a check for $1160
} } so that I can sign contracts and purchase sodas and supplies locally.
} } Rosemary
} } --
} } Rosemary Walsh, Manager
} } The Electron Microscope Facility for the Life Sciences,
} } A Shared Technology Facility, The Life Sciences Consortium
} } 1 South FrearLab
} } Penn State University
} } University Park, PA 16802
} } (814) 865-0212
} } rw9-at-psu.edu
} } http://www.lsc.psu.edu/stf/em/home.html

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Mon May 14 07:47:21 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 14 May 2001 09:20:25 -0400
Subject: Re: Fund transfer to cover dinners

Contents Retrieved from Microscopy Listserver Archives
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} That is fine with me, Hong. I have given Stacie the information on
} where to send it.
Rosemary




} Rosemary, I will have Stacie to cut you a check for $2,400.00. That will
} include the fee for using your facility and food money. Is that OK with
} you? Let me know so I can call Stacie soon. Thank you.
}
} Hong
} ----------
} } From: Rosemary Walsh {rw9-at-psu.edu}
} } To: Hong Yi {hyi-at-emory.edu}
} } Subject: Fund transfer to cover dinners
} } Date: Fri, May 11, 2001, 11:24 AM
} }
}
} } Hi Hong,
} } After consulting with our admin. assistant, it would be best
} } to have Stacie send me a check for $1160
} } so that I can sign contracts and purchase sodas and supplies locally.
} } Rosemary
} } --
} } Rosemary Walsh, Manager
} } The Electron Microscope Facility for the Life Sciences,
} } A Shared Technology Facility, The Life Sciences Consortium
} } 1 South FrearLab
} } Penn State University
} } University Park, PA 16802
} } (814) 865-0212
} } rw9-at-psu.edu
} } http://www.lsc.psu.edu/stf/em/home.html

--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Mon May 14 09:32:15 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Mon, 14 May 2001 10:25:55 -0400
Subject: science project assistance

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

My son is doing a science project where he needs to contact a researcher
working with Multiple Sclerosis and ask some questions. He has not had
any responses on his previous email attempts to contact someone, so I'm
turning to the listserver (even though it's a little off-topic).

Can anyone put me in touch with an MS researcher who would be able to take
a few minutes to respond to questions from a 13 year old?

Thanks,
Henk Colijn
Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Mon May 14 10:37:28 2001



From: Gary Gill :      garygill-at-dcla.com
Date: Mon, 14 May 2001 10:13:59 -0500
Subject: Troubleshoot visual blurring during microscopy of acellular field

Contents Retrieved from Microscopy Listserver Archives
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Can anyone account for the cause and solution to this problem?

Background:

We have installed a custom reticle in the eyepieces of our
cytotechnologists' microscopes. Looking through the microscope, one sees a
clear square area outlined by neutral density filter-like segments. The
corners of the square just touch the boundaries of the field-of-view. In
the center, there are a 6.7 micrometer diameter circle and 2 crossed 11
micrometer long lines for use as size comparators.

The square provides a user-friendly frame of reference by which a
cytotechnologist can advance a Pap smear the length of 1 side while
screening, using a 10x objective and 10x eyepieces. This systematic
step-size movement promotes more complete and reproducible screening
coverage, and initially has resulted in an increase in the pick-up rates for
various categories of abnormal cells. The two size comparators are intended
for use with a 40x objective to assist cytotechnologists when estimating
nuclear areas and potential significance.

Problem:

One cytotechnologist in particular complains that her non-dominant eye
"blurs over" when she encounters acellular fields-of-view. Despite patient
persistence in working through this effect, she eventually gave up, as this
effect was causing her to have headaches. I have removed the reticle. A
few other cytotechnologists have complained similarly, but they have learned
to tolerate the annoyance. The overwhelming majority of users have not
experienced this problem.

Hypothesis:

I don't know why the reticle is causing this one user's eye to "blur over"
(her words). However, I suspect that when both eyes are looking at cellular
fields-of-view, both are focused at the same distance. However, upon
encountering acellular fields, the dominant eye focuses on the two size
comparators, while the non-dominant eye focuses at a different distance.
Thus the brain is receiving mixed signals. To test this hypothesis, I've
returned a reticle to the manufacturer to remove the size comparators. I'll
reinstall this modified reticle and see whether the blurring over persists.
If not, then problem solved. If yes, then the user will have to screen
without the benefit of the reticle. I've also asked the lady to ask her
ophthalmologist to write down her visual status.

Can anyone explain the cause and prevention of this phenomenon? Thanks.

Gary


From daemon Mon May 14 12:30:23 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 14 May 2001 12:08:16 -0500
Subject: Job opening - LM facility Associate Director

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The University of Missouri is searching for an Assistant or Associate
Director of their Molecular Cytology Core Facility. This facility
deals with all types of light microscopy. Individuals who feel they
may be qualified for the position are encouraged to either apply or
contact Tom Phillips for further details. A description of the job is
appended to this message.

Assistant or Associate Director
Molecular Cytology Core Facility

The Molecular Biology Program at the University of Missouri is
seeking an individual with experience in some or all of the following
areas:

* confocal scanning laser microscopy
* bright field microscopy
* wide field fluorescence microscopy
* deconvolution
* image processing/analysis
* all types of microtomy
* immunocytochemistry
* in situ hybridization
* Adobe Photoshop


The Assistant/Associate Director will be responsible for training
users, maintaining instruments and developing protocols for a
campus-wide multi-user facility. PhD desirable but not required for
individuals with extensive experience. Although an ideal candidate
would have experience in all of the areas listed above, candidates
with extensive experience in selected areas and who have the desire
and capacity to learn the additional areas will be considered.
Excellent oral and written communication skills are essential.
Experience in a multi-user core facility would be viewed positively.
Electron microscopy is not a component of this core facility. Women
and minority candidates are especially encouraged to apply. Review of
applications will begin immediately and continue until an appropriate
candidate is hired.

The Core's web site can be viewed at
http://www.biotech.missouri.edu/mbp/cores/index.html

Address applications (CV and 3 letters of reference) or inquires to:

Thomas E. Phillips, Ph.D.
Division of Biological Sciences
3 Tucker Hall, University of Missouri
Columbia, MO 65211-7400.
573-882-4712
PhillipsT-at-missouri.edu.


From daemon Mon May 14 15:44:27 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 14 May 2001 13:59:41 -0700
Subject: Re: digital watermarking

Contents Retrieved from Microscopy Listserver Archives
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I am so sorry Gordon. I was answering the question of David Foran what
digital watermark is. I did not figured out that the question was related
to forensic. But, my position is still the same: "watermarks" are about
copyrights protection and very slightly related to the
image-quality. You may easily present to the court the image and nobody
(except author) will know that it's watermarked. Using very simple
program, author may verify his/her authority on this image at the court.
Because this technology has been protected by at least 5 patents and
registered, court would accept such evidence, I believe. This what purpose
of digital watermarks in my opinion. The same way, some detective may
watermark his images with some evidence and present it to court. Watermark
in this case will not verify the trustness of the images, but authority of
this particular person on the images. The same: some Laboratory (even
forensic) may use watermarks to identify their digital products.

I do agree with you, that it's so easy to manipulate image digitally
nowadays. From another hand, we do have an arsenal of the old nice tricks
how to modify negatives/slides as well. People who prefer to stick to
"film" will probably agree with me that it's very possible to modify images
on the film too. Simplest example: using "ferro-cyanide" (not sure the
spelling is correct) you may partially/completely eliminate part of the
image on the wet negative/paper. After washing - the modification is
undetectable in practice. Using this way, you could, for instance, remove
some bands from electrophoresis pictures, or using multiple exposure -
enhance some.

Sergey.


At 04:08 AM 5/14/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 14 18:19:16 2001



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Mon, 14 May 2001 16:12:00 -0700
Subject: EM Facility Charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers. We are in the midst of trying to set up a new EM facility
here. I am wondering how many of you out there actually support your
facility with users fees? Is it realistic to expect service contracts,
personnel salaries, supplies and maintenance can be recouped from fees? Or
do most facilities need to be subsidized to ensure their survival? I worry
that the kind of fees we would have to charge to ensure survival will scare
many users away. Any suggestions, comments would be appreciated.

JoAnn Buchanan
Dept. Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305



From daemon Tue May 15 06:05:38 2001



From: William R. Oliver :      oliver-at-cpt.afip.org
Date: Tue, 15 May 2001 06:15:13 -0400 (EDT)
Subject: Re: digital watermarking

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That's not as big an issue as a lot of people make it out to be. Yes, you have
to show the provenance of an image in court, and be able to describe and
defend the kind of processing you do.

However, the bottom line is that the image is *not* the testimony. The *testimony*
is the testimony. It has *always* been possible to manipulate images; the
ease in doing it is secondary. The important thing is that you have an
expert sitting there and stating under oath that the image does or does
not accurately represent what it is supposed to represent.

The assumption that the expert is lying unless the image itself can be
independenly proven to accurately represent what the expert says
is simply wrong. Images, except in specific circumstances, are there
to *illustrate* observations made by the expert.

Consider scene photography. The homicide cop gets up and says there
was a piece of paper on a table. There is a photograph of a piece of
paper on a table. Counsel asks "Is this a fair and accurate
representation of where the paper was?" The cop replies "Yes, it is."

Now, defense may get up and say "Aha! That's a digital image! Somebody
could have *put* that image of paper on that table."

The answer is "Yeah, they could have. But the cop here says that's
where it was. The cop here says that's where he saw it. The cop here
says this here's what it looks like." The jury will either believe
the cop or they won't.

One can think of cases where a photograph *doesn't* fairly and
accurately represent what the cop saw -- the most common example I have
seen is in color balance problems where the cop says the sweater was
"green" and the photo shows it blue. Then you either don't enter it
into evidence or you do the appropriate testimony. In other cases,
image processing is integral to the conclusion itself -- such as edge
enhancement or deblurring -- in which case you have to detail and defend
the algorithms. But even then, that justification is based on testimony
of the expert, not on some feature measurement of the image, and the "ease"
with which it was done is fairly irrelevant.

If the court rejects testimony on the basis of some secondary measure
of accuracy other than the testimony of the expert witness, then the
lawyers have not done their job. The bullshit job that the defense did
in the OJ trial is *not* the paradigm of how a trial should work, and
should not be the standard for how evidence is examined.

billo



On Mon, 14 May 2001, Gordon Couger wrote:

}
}
} Dear Sergey
}
}
} My point being that I would be very nervous using a digital image in court
} because of the ease that they can be manupulated and the fact that there
} is no way to tell if it is orignal or not.
}
} Gordon
}
}
}



From daemon Tue May 15 07:25:16 2001



From: rj.carey-at-student.qut.edu.au ()
Date: Tue, 15 May 2001 07:21:43 -0500
Subject: Ask-A-Microscopist: sample prep for malignant melanoma?

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Below is the result of your feedback form. It was submitted by
(rj.carey-at-student.qut.edu.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, May
15, 2001 at 02:44:36
---------------------------------------------------------------------------

Email: rj.carey-at-student.qut.edu.au
Name: richard carey

Organization: queensland university of technology

Education: Undergraduate College

Location: brisbane.queensland,australia

Question: A skin nodule resected from a patient is suspected of being
a malignant melanoma.How would I prepare and view the sample to
determine if this was the case?

---------------------------------------------------------------------------


From daemon Tue May 15 07:31:56 2001



From: Bobrowski, Walter :      Walter.Bobrowski-at-pfizer.com
Date: Tue, 15 May 2001 08:17:08 -0400
Subject: Image Analysis - LCD Tablet

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I would appreciate from users of the Wacom PL series LCD writable tablet,
for use specifically with image analysis, not graphics arts. The ability to
trace directly on the image rather than using a mouse or pen/tablet
combination seems appropriate but would like some feedback. TIA.

Walter F. Bobrowski
Electron Microscopy Laboratories
Drug Safety Evaluation
Pfizer Global Research and Development
2800 Plymouth Road
Ann Arbor MI 48105

TEL: (734) 622-7814
FAX: (734) 622-3478
MOBILE: (734) 646-0502


From daemon Tue May 15 07:45:43 2001



From: Leslie Eibest :      leibest-at-duke.edu
Date: Tue, 15 May 2001 08:41:44 -0400
Subject: Re: EM facility charges

Contents Retrieved from Microscopy Listserver Archives
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JoAnn;

Our SEM facility staggered along for years while supported
partly from the grants of our five principal users and partly from
user fees. This was not a stable system, since grant support dried
up and usership was erratic.

Now our service contract and technician's salary are
subsidized by three departments, and all members of these departments
have free use of the facility and technical assistance. Fees from
users outside of these departments are used to purchase supplies and
for equipment repair/upgrades. These fees can be kept at a
reasonable level, and members of the subsidizing departments love
having free access to the lab. If you can arrange some sort of
subsidy (and it is not too much of a burden if shared among
departments), it is a great way to go.

Leslie Eibest

At 4:12 PM -0700 5/14/01, JoAnn Buchanan wrote:
} Is it realistic to expect service contracts, personnel salaries,
} supplies and maintenance can be recouped from fees? Or do most
} facilities need to be subsidized to ensure their survival?


From daemon Tue May 15 08:33:02 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Monday, May 14, 2001 6:12 PM
Subject: Fwd: EM Facility Charges

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Joann,
We will, hopefully, get started on a new pricing scheme in June. We have recently combined a small service facility from the Vet School with a multi-user facility that I have managed for, primarily, the School of Agriculture. The Ag. School has picked up all costs for service contracts and misc. to date including half of my salary (other half picked up by School of Science). Thus there was no charge, except for consumables, for use of this facility over the past 17 years. Now they want us to charge to try to recoop some of the costs. We run into problems that if you charge labor (for service) than you have to pay all the employees fringe benefits which are usually paid by the University. This adds about 1/3 to the cost of the employee to the department/school. Thus fees may actually cost the department/school more if you do not bring in enough money.

Thus there is the problem of how do you charge for service that will fairly compensate for the employee's time while still not penalizing the multi-user who works independently. Also, if you charge too much for imaging time you will tend to reduce use of equipment and it costs the same for service contracts whether booked full time or only part-time.

Consumables are not a problem since we can figure that fairly easily and multi-users provide most of their own reagents, etc.

I would like to consider an approach that would have departments pick up a share of the costs based on the number of faculty and their students who have used the facility over the last 6 months or so. They could pass that on to the researchers if they wanted to... Since we have over 15 departments who use this facility each year, an average of $1500 per department would go a long way to covering service contracts...especially if the Deans of the 5 schools involved would also toss in another couple of thousand each. Consumable reimbursement and very modest beam charges (~$10/hr) would cover misc. expenses while charge for labor for those using service would help defray costs of one technical person.

I would be very interested to hear if anyone has tried a plan such as this to help cover costs. I do know that it is used at one university in a large computer lab. Everyone who uses the lab is charge $50/month. Thus they have essentially unlimited use for that month. If they only use the facilities of 1 month, they are charged accordingly. If more than 1 person from a lab uses the facility within the same month, they are still charged per person. Apparently it works quite well as they make enough money to cover equipment upgrades, although not necessarily salaries.

Please do compile and send a summary of your responses to the list since this is a topic that is of continual interest to both lab managers and users.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907

--------------------------------------


Dear Listers. We are in the midst of trying to set up a new EM facility
here. I am wondering how many of you out there actually support your
facility with users fees? Is it realistic to expect service contracts,
personnel salaries, supplies and maintenance can be recouped from fees? Or
do most facilities need to be subsidized to ensure their survival? I worry
that the kind of fees we would have to charge to ensure survival will scare
many users away. Any suggestions, comments would be appreciated.

JoAnn Buchanan
Dept. Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305





From daemon Tue May 15 10:09:39 2001



From: Young Ho Koh :      younghokoh-at-facstaff.wisc.edu
Date: Tue, 15 May 2001 10:04:50 -0500
Subject: information about ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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Dear Listservers,
I am now looking for a resonable ultramicrotome to buy. Does anybody know
where I should look for used ultramicrotomes and diamond knifes?
Thank's a lot in advance.

Sincerely,

Young Ho Koh, Ph.D
University of Wiconsin Madison,
445 Henry Mall
Madison, WI 53706


From daemon Tue May 15 12:31:56 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Tue, 15 May 2001 14:23:39 -0300
Subject: Critical Point Driers

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What is the available these days in terms of Critical Point Driers. Ours
seems to be on its last legs and we will possibly replace it. Manufacturers
and sales welcome.

-David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford Street
Halifax NS Canada B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca


From daemon Tue May 15 12:37:11 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 15 May 2001 10:32:25 -0700
Subject: Re: EM Facility Charges

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Dear JoAnn,
That is always the dilemma. To fully recoup the costs of running an EM
facility, you would have to charge at least $200 per hour for an SEM and
$400 per hour for a TEM, and hope they were booked 60% of the time. Most
researchers cannot afford that. Most university EM labs will charge
university researchers much less than that, but then the university must
subsidise the costs. I charge researchers $25 per hour SEM and $50 per hour
TEM and that covers consummables and small incidental costs, but not service
contracts (I don't have any) or my salary. I get some commercial work at
commercial rates, but that is less than 5% of my time.
One other point, a university with a good EM facility can use that fact to
attract more research funding. There is a lot of research in many diverse
fields that is enhanced or facilitated by the access to modern EMs.
At 04:12 PM 5/14/01 -0700, you wrote:

}
}
} Dear Listers. We are in the midst of trying to set up a new EM facility
} here. I am wondering how many of you out there actually support your
} facility with users fees? Is it realistic to expect service contracts,
} personnel salaries, supplies and maintenance can be recouped from fees? Or
} do most facilities need to be subsidized to ensure their survival? I worry
} that the kind of fees we would have to charge to ensure survival will scare
} many users away. Any suggestions, comments would be appreciated.
}
} JoAnn Buchanan
} Dept. Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
}
Regards and luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue May 15 13:56:04 2001



From: Platek, Frank :      FPLATEK-at-ora.fda.gov
Date: Tue, 15 May 2001 14:50:38 -0400
Subject: RE: Digitized images as court evidence

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I have resisted diving into this issue of digitized/computer enhanced images
and their use as trial exhibits as a number of good comments have been made
by other well qualified scientists. For the record, I would like to note
that I HAVE used digitized images and computer-processed and enhanced images
(both photographs and photomicrographs) in Federal Court testimony. As
several other responses to the list have noted or expressed a concern that
the images might be challenged, I can assure you that they (the images) WERE
questioned. Defense council objected to several computer "enhanced" or
"highlighted" images which had been submitted as exhibits to support my
testimony. Defense council further wanted all such images rendered
inadmissible. The judge allowed me to explain to the court exactly what had
been done to the questioned digital images as part of my case analysis.
Though the defense council turned my phrases from "digitally recorded" and
"digitally enhanced" into derogatory slurs of "digitally manipulated" and
"computer-altered", the judge listened patiently to my remarks on computer
digital imaging and image analysis. He then stated he could find no problem
with the processes I had described and no grounds to dismiss the exhibits
which he allowed to stand.

Those of us from the old "red light / dark room" days know it is very
possible to "dodge" out specific regions of a photograph or "super impose"
an object into a photograph which was never there in the first place.
Although it took a little more time than a few keystrokes at the computer,
one could achieve very convincing photographic images from the trays of the
darkroom. Furthermore, in the BC (before computers) days, you seldom had to
swear in court, other than your original oath, that you had not altered the
images in any manner.

The credibility of the analyst / microscopist / scientist / forensic
scientist/ responsible individual is the ONE THING we MUST preserve and do
everything in our power to maintain.

Our credibility is the backbone of our science.

Therefore, do your scientific analysis and record ALL that you do to the
evidence (i.e., images).
Truthfully, report your findings and observations based upon YOUR analysis
and don't "over step" your results.
Be a man/woman of unquestionable character.
Allow the judicial process to take it's course. (what ever course that is)
THEN:
To slightly paraphrase Bill Oliver's recent post, "The jury will either
believe you or they won't."
At that point you have done your job.

I fully realize many of the list readers are not forensic scientists and
will seldom be concerned with court testimony, but permit me one last
observation. Several years ago and at my request, Mr. Robert Hathaway of
the Rhode Island State Crime Laboratory provided me with a copy of
Brouardel's Statement about the role of the Forensic Scientist which Mr.
Hathaway used in his presentation. I have had it on my office AND
laboratory wall ever since. (Thanks Bob; it helps to keep things in
perspective.)

" If the Law has made you a witness, remain a man of science. You have no
victim to avenge, no guilty or innocent person to convict or save. You must
bear testimony within the limits of science. " Dr. P.C. Boarded


Respectfully submitted,

S. Frank Platek
Forensic Chemistry Center
U.S. Food and Drug Administration
6751 Steger Drive
Cincinnati, OH 45237-3097
(513) 679-2700 X254
(513) 679-2761 FAX
fplatek-at-ora.fda.gov

Disclaimer: The opinions and views expressed above are those of author and
do not necessarily represent
those of the US Food and Drug Administration or any
other Federal or State Agency.


















From daemon Tue May 15 15:52:00 2001



From: Pbgrover-at-aol.com
Date: Tue, 15 May 2001 16:45:55 EDT
Subject: Proto-Slo

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Dear Listers,

I have some 5-to-8 year-olds who really enjoy the microworld, but whose hands
can't keep up with speedy microbeasties under the lens. I remember from
years past that there was a product (I think it was called 'Proto-Slo', or
similar) that was viscous, roughly iso-osmotic, and designed to slow the
critters down for easier observation. Is it still available? If not, does
anyone have a recipe for a home-brewed equivalent?

Thank you, thank you, thank you,

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN


From daemon Tue May 15 16:23:47 2001



From: Xinran Liu :      xinran.liu-at-utsouthwestern.edu
Date: Tue, 15 May 2001 16:16:51 -0500
Subject: Immuno EM on human brain

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Hi, Colleagues,

We are doing immuno EM labeling experiment on formalin fixed human brain
(the tissue has been fixed for more than 2 years). The labeling density is
very minimal. The same antibody we have used on mice without any problem.
Does anyone have experience on how to optimize the formalin fixed human
tissue for immuno electron microscopy? Thank you in advance.

Xinran Liu, M.D., Ph.D.
Assistant Professor
Center for Basic Neuroscience
UT Southwestern Medical Center at Dallas
Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu



From daemon Tue May 15 19:29:21 2001



From: anton.j.bons-at-esso.com
Date: Tue, 15 May 2001 19:24:36 -0500
Subject: TEM SEM Postdoctoral position - ExxonMobil Chemical Belgium

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Please consider the following exciting position with ExxonMobil Chemical in
Europe. If you are interested, please e-mail or contact Dr. Anton-Jan Bons
in Machlen, Belgium at address anton.j.bons-at-esso.com

Please do not send replies to Mark Disko in the USA.



===========================================================

2-YEAR POSTDOC POSITION:
TEM CHARACTERIZATION OF CATALYTIC MATERIALS

ExxonMobil Chemical Europe Inc., European Technology Center
Machelen (near Brussels), Belgium

A 2-year post-doctoral research position is currently available in the
Catalytic Materials Group of Basic Chemicals Technology. The group focuses
on the synthesis, characterization and testing of zeolites and related
materials, for application in ExxonMobil's production processes. The current
position involves structural characterization by Transmission Electron
Microscopy and other techniques. Facilities available in the group include
TEM, FEG-SEM, EDX and XRD; a multitude of other characterization techniques
is available at other ExxonMobil sites and at nearby universities.
Candidates should hold a doctoral degree in physical sciences, with a
strong affinity for electron microscopy, diffraction and/or crystallography.
Further information can be obtained from Dr. Anton-Jan Bons, tel. +32 2 722
2838, email anton.j.bons-at-esso.com.
Applications including a full CV, list of publications and name and
addresses (including e-mail) of three references should be sent to
ExxonMobil Chemical Europe, Human Resources Department, Hermeslaan 2, B-1831
Machelen, Belgium, as soon as possible.


From daemon Wed May 16 07:45:19 2001



From: Simon Dumbill :      simon.dumbill-at-aeat.co.uk
Date: Wed, 16 May 2001 07:32:46 -0500
Subject: Philips EM430 available

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Due to a rationalisation of facilities, AEA Technology is disposing
of a Philips EM430 TEM/STEM.

The microscope is a 300kV instrument equipped with an Oxford
Instruments EXL-II EDX system and is available with a full range of
sample holders, including analytical double-tilt, heating stage, LN2
double tile cold stage and a straining stage. The instrument has been
under a full service contract since new.

For further details, please contact me directly.

Thanks,
Simon



{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}

Dr Simon Dumbill
Team Leader, Microstructural Characterisation
AEA Technology Nuclear Science
B7, Windscale
Seascale
Cumbria CA20 1PF

Tel: +44 (0)19467 72235
Fax: +44 (0)19467 72606

Email: Simon.Dumbill-at-aeat.co.uk

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}


From daemon Wed May 16 07:58:12 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 16 May 2001 08:53:51 -0400
Subject: Re: Critical Point Driers

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Dear David O'Neil,

Ladd Research, as well as some of the other supply houses, sell CPDs. To
check out ours, please go to
http://www.laddresearch.com/New_Products/CPD/cpd.html

Thanks,

JD Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


O'Neil, David wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} What is the available these days in terms of Critical Point Driers. Ours
} seems to be on its last legs and we will possibly replace it. Manufacturers
} and sales welcome.
}
} -David O'Neil
} Institute for Marine Biosciences
} National Research Council of Canada
} 1411 Oxford Street
} Halifax NS Canada B3H 3Z1
} ph. 902-426-8258
} fax 902-426-9413
} david.o'neil-at-nrc.ca


From daemon Wed May 16 09:42:30 2001



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 16 May 2001 10:34:58 -0400
Subject: Symposium announcement...

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} Listers (especially those in the Southeast USA): Please check out
} the details of this symposium at the web link given below.
}
Larry






}
} Materials MicroCharacterization: Today and Tomorrow
}
} ASM International, Oak Ridge Chapter
} Educational Symposium 2001
}
} The development of new materials with novel physical and mechanical
} properties will play an important role in the twenty-first century
} economy. Nation-wide initiatives aimed at developing nanostructured
} and other complex advanced materials have been instituted. To
} develop the scientific understanding today that will tomorrow enable
} the synthesis of these new classes of materials, characterization
} will play a central role. The relationship between novel materials
} properties and the processing methods used in their synthesis will
} depend on determining the structure, composition and chemical
} bonding of these materials at length scales from the atomic to the
} mesoscale. This year's Educational Symposium will highlight the
} state-of-the-art and future directions of a variety of materials
} microcharacterization techniques. A mix of well-established and
} recently developed techniques will be covered.
}
} The Symposium will be held at the Pollard Auditorium in Oak Ridge,
} as single-day event with internationally recognized speakers from
} all over the country. The date of the Symposium is Thursday, May
} 31, 2001. The registration is a low $100 for professionals, and $20
} for students. The registration fee includes a morning welcoming
} continental breakfast, two coffee breaks, and a catered lunch.
} Registrations can be confirmed by e-mail to Cheryl Lee at
} cslee-at-ornl.gov. Checks should be made out to "ASM Oak Ridge
} Chapter" with "Educational Symposium 2001" noted in the memo line,
} and mailed to the address below. Additional information can be found
} at the web site of the ASM Oak Ridge Chapter at
} http://www.korrnet.org/orcasm/.
}
} Speaker List:
}
}
} Larry Allard, Oak Ridge National Laboratory
} Peter Nellist, Nion Co.
} Tom Kelly, University of Wisconsin
} Greg Rohrer, Carnegie Mellon University
} Joe Michael, Sandia National Laboratory
} Harald Ade, North Carolina State University
} Kent Childs, Phi Co.
} Paul Carpenter, NASA Marshall Space Flight Center
} David Joy, University of Tennessee

--
Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Wed May 16 09:48:42 2001



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 16 May 2001 09:43:22 -0500
Subject: Oil Red O lipid staining

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Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
(Epon or Epon-Araldite)? If so, has this been done by staining en bloc or
by staining the sections. Sections would range from 1-4 microns in
thickness.

We would also consider tissues embedded in glycol methacrylate. We'd like
to avoid frozen sections because we'd prefer the higher level of detail
possible with plastic.

Thank you,

Jaclynn Lett, Research Assistant jlett-at-cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110



From daemon Wed May 16 09:52:40 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 16 May 2001 09:24:25 -0500
Subject: Re: Image Analysis - LCD Tablet

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FWIW, we have been using a Wacom tablet to trace outlines for image
analysis. We simply used the image on the screen to guide our hand on the
tablet. It really didn't take too long to develop the necessary
coordination. I wonder if you would have any trouble with parallax with the
LCD tablet.

The tablet has two modes of operation: typical mouse motion (relative) and
screen coordinates (absolute). We had to be sure to run in screen
coordinates mode to get the best performance tracing. We also had to make
sure our image window was filling a large portion of the screen to get the
least amount of jaggies (due to either shaking hands or limited tablet
resolution) while tracing. Bigger tablets are better in that regard. We
were working with a 5x7" tablet which was fairly inexpensive.

Warren S.

At 08:17 AM 5/15/2001 -0400, you wrote:

} I would appreciate from users of the Wacom PL series LCD writable tablet,
} for use specifically with image analysis, not graphics arts. The ability to
} trace directly on the image rather than using a mouse or pen/tablet
} combination seems appropriate but would like some feedback. TIA.
}
} Walter F. Bobrowski
} Electron Microscopy Laboratories
} Drug Safety Evaluation
} Pfizer Global Research and Development
} 2800 Plymouth Road
} Ann Arbor MI 48105

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Wed May 16 10:14:55 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 16 May 2001 10:14:17 -0500
Subject: FIB: grain size analysis software?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listies: we are preparing samples for Cu grain size
analysis with a focused ion beam and then doing the analysis
manually. Engineering would like to know if there is any
software out there that will do this automatically or with
little user intervention. Vendors can respond to me
off-list.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
DSPS Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed May 16 10:57:57 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 16 May 2001 08:51:14 -0700
Subject: Re: Proto-Slo

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
We used to use glycerin in the Microbiology lab. Only way to see Chlamydomonas.
At 04:45 PM 5/15/01 EDT, you wrote:

} Dear Listers,
}
} I have some 5-to-8 year-olds who really enjoy the microworld, but whose hands
} can't keep up with speedy microbeasties under the lens. I remember from
} years past that there was a product (I think it was called 'Proto-Slo', or
} similar) that was viscous, roughly iso-osmotic, and designed to slow the
} critters down for easier observation. Is it still available? If not, does
} anyone have a recipe for a home-brewed equivalent?
}
} Thank you, thank you, thank you,
}
} Paul Grover
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 16 11:05:26 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 16 May 2001 09:00:46 -0700
Subject: Re: Proto-Slo

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Paul -

Questions like this can usually be answered wth a quick look at the
Carolina Biological (or equivalent) catalog. Protoslo is still there.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed May 16 13:14:19 2001



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Wed, 16 May 2001 13:18:48 -0500
Subject: Re: Image Analysis - LCD Tablet

Contents Retrieved from Microscopy Listserver Archives
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Hi Warren:
I am also interested, about how much are the small tablets?
Thx,
Mike Coviello
UT Arlington

Warren E Straszheim wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} FWIW, we have been using a Wacom tablet to trace outlines for image
} analysis. We simply used the image on the screen to guide our hand on the
} tablet. It really didn't take too long to develop the necessary
} coordination. I wonder if you would have any trouble with parallax with the
} LCD tablet.
}
} The tablet has two modes of operation: typical mouse motion (relative) and
} screen coordinates (absolute). We had to be sure to run in screen
} coordinates mode to get the best performance tracing. We also had to make
} sure our image window was filling a large portion of the screen to get the
} least amount of jaggies (due to either shaking hands or limited tablet
} resolution) while tracing. Bigger tablets are better in that regard. We
} were working with a 5x7" tablet which was fairly inexpensive.
}
} Warren S.
}
} At 08:17 AM 5/15/2001 -0400, you wrote:
}
} } I would appreciate from users of the Wacom PL series LCD writable tablet,
} } for use specifically with image analysis, not graphics arts. The ability to
} } trace directly on the image rather than using a mouse or pen/tablet
} } combination seems appropriate but would like some feedback. TIA.
} }
} } Walter F. Bobrowski
} } Electron Microscopy Laboratories
} } Drug Safety Evaluation
} } Pfizer Global Research and Development
} } 2800 Plymouth Road
} } Ann Arbor MI 48105
}
} ----------------------
} Warren E. Straszheim
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking



From daemon Wed May 16 17:22:36 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 16 May 2001 17:15:01 -0500
Subject: colloidal gold conjugates

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We need a 3nm colloidal gold conjugated to strepavidin. You can now purchase conjugates at 5, 10, etc in size but not 3nm so we plan to attempt to make it. I have made 5 and 10 nm conjugates using IgG's and protein-A which turned out great and do have the instructions for the 3nm colloid. I was wondering if anyone had made a strepavidin conjugate and, if so, would you share your procedure for the conjugation? I do not know if the procedure used for IgG's would work equally well for strepavidin.
Thanks,
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



From daemon Wed May 16 18:03:40 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 16 May 2001 16:05:58 -0700
Subject: RE: Digitized images as court evidence

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Very well said, Frank.

Thanks.

Gary G.



At 11:50 AM 5/15/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed May 16 18:25:05 2001



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Thu, 17 May 2001 01:21:17 +0200
Subject: image resolution...

Contents Retrieved from Microscopy Listserver Archives
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Hello Listservers

I am in doubt by one of our customers - specialist in image
manipulations/publications.
The talk is about "resolution of an digital image" - in it's virtual form -
means - in a datafile format - e.g. on floppy.
According to my understanding - it is described as pixel dimention of an
image e.g. 1200x800 pixels + pixel digital depth e.g. 8 bit (for grayscale -
256 gray levels).
This immediately determines the raw image size as uncompressed bitmap.
E.g. the digital camera images resolution is defined in Megapixels defining
the sensor matrix - which means - pixel X times pixel Y dimentions of
image - multiplicated - independent of image ratio (rectangular, square
etc.) This is understandable and clear terminology.

However the "image analysis" people,I am facing now, use the DPI or PPI
parameter as a resolution measurment.

Talking about the shortcut meaning "dots per inch" or "pixels per inch" (as
far as I understand it) - has only the sense when there is a specific media
described/selected - monitor working at particular grafic card resolution,
scanner sampling with specific optical sensor resolution, hardcopy
(printout, photo, negative or videoprint) having specific size and printing
head resolution....etc.
This "inch" must be defined somwhere in physical way.

Some people told me that ANY image in datafile format has own RESOLUTION
defined in this DPI's or PPI's... ?????
How this should be understood ???

It is for me an absurd until one specifies the physical media where this
"inch" is defined.

PLEASE advise with Yr experience - is that right or not - how the resolution
specified in DPI should be related to image pixel per pixel resolution.

kind regards, awaiting clarification....

Krzysztof Herman
EMISJA - FEI EO Poland
ul.Ba¿ancia 45A
02-892 Warszawa
tel/fax: (+48 22)6449753, 6449750
mobile: (+48 601)307456
kherman-at-labsoft.com.pl
www.emission.com.pl




From daemon Wed May 16 19:32:40 2001



From: Greg Lum :      glum-at-sfsu.edu
Date: Wed, 16 May 2001 17:30:26 -0700
Subject: info on used ultramicrotomes & diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MSA Listserver is a good source for used ultramicrotomes, but you'll
have to do some searching.

Microscopy Today magazine has a used equipment for sale column toward
the back. See http://www.microscopy-today.com.

Used diamond knives: It would cost a couple thousand to resharpen
used ones. You might try Microstar Technologies in Texas for new
ones: phone 409-291-6891, email: mistar-at-msn.com for a catalog.

I'm not affiliated with those enterprises.

Greg
--
Greg Lum
Computer/Microscopy Consultant
Dept of Biology
San Francisco State University
Ph: 415/338-1339
email: glum-at-sfsu.edu


From daemon Wed May 16 19:32:41 2001



From: Paul Webster :      pwebster-at-hei.org
Date: Wed, 16 May 2001 17:27:20 -0700
Subject: LM: Low cost inverted microscopes?

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

A friend is looking for a low cost inverted light microscope (ca $15,000).
I am not very knowledgeable about what is available so look to you for
advice.

She was particularly interested in something equivalent to the Zeiss
Axiovert 100, 135, or even better, the 100TV.

I do know these are no longer being supplied by Zeiss so the names of
reliable sources for used microscopes would be useful.

All information supplied will remain confidential, although I may post a
list of useful contacts back onto this forum.


Regards,

Paul Webster


Paul Webster, Ph.D.
Scientist II and Director
Ahmanson Center for Advanced EM & Imaging
House Ear Institute
2100 West 3rd Street
Los Angeles
CA 90057

pwebster-at-hei.org
p: 213 273 8026
f: 213 413 6739
http://www.hei.org/aemi.htm

Joke for the day: "Does radioactive halibut make good fission chips?"






From daemon Thu May 17 00:51:45 2001



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Thu, 17 May 2001 14:43:38 +0900
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krzysztof,

} Some people told me that ANY image in datafile format has own RESOLUTION
} defined in this DPI's or PPI's... ?????
} How this should be understood ???

Some image formats include such information in the file. This allows one to
have an idea about the physical dimensions of the original object.
If only the value of DPI or PPI is specified for an image then it is not
possible to relate it to image pixel resolution.
Both DPI and size of the image in inches should be specified, then:

ImageSizeInPixels = ImageDPI * ImageSizeInInches

Hope this makes sense.

Best regards,

Rado



From daemon Thu May 17 02:20:42 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Thu, 17 May 2001 08:16:28 +0100
Subject: Fw: image resolution...

Contents Retrieved from Microscopy Listserver Archives
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Krzystzof
You have defined two aspects of "resolution" in your question, (1)
pixel resolution relating to the sensor resolution) (2) pixels per
inch on the display or printer output. A third is pixels per inch of
real-world dimensions of the object being imaged. This is the
spatial
calibration or spatial resolution, and must be known if the sizes of
objects in the image are to be measured by image analysis.

When you scan a paper image (e.g. a photographic print) on a flat-bed
scanner into e.g. Adobe Photoshop, Photoshop records pixels x,y and
pixels per inch in the file header. Then when the image is printed it
emerges from the printer the same size as the original. In this
particular case, resolution types 2 and 3 are initially the same. But
both pixels x,y and ppi can be altered using Photoshop's image size
dialog. If either are changed then the relationship of Type 1 and
Type
2 resolution to Type 3 resolution is changed, and it will no longer
be
possible to determine an object's dimensions. If a digital camera or
microscope image of a real-world scene is captured via a Twain
grabber
into Photoshop, Photoshop will report pixels x,y and also pixels per
inch for such an image. It seems to define ppi by scaling the input
pixels x,y into the currently-defined page, so 768x576 pixels comes
out as 72 ppi on A4 paper. But the type 3 resolution, real-world
pixels per inch of objects in the image, cannot be calculated until
some object of known dimensions is imaged under the same conditions.

The problem here is that we tend to use the same word "resolution" to
mean any of these things without specifying which. We should perhaps
use a second qualifying word such as sensor resolution, pixel
resolution, image resolution, print resolution, spatial resolution.
This helps, but you can see from these examples that it is hard to
avoid ambiguity.

Chris

} ----- Original Message -----
} From: "Krzysztof Herman" {kherman-at-labsoft.com.pl}
} To: "MSA" {Microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, May 17, 2001 12:21 AM
} Subject: image resolution...
}
}
} --------------------------------------------------------------------
--
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
--
} -.
}
}
} Hello Listservers
}
} I am in doubt by one of our customers - specialist in image
} manipulations/publications.
} The talk is about "resolution of an digital image" - in it's virtual
} form -
} means - in a datafile format - e.g. on floppy.
} According to my understanding - it is described as pixel dimention
of
} an
} image e.g. 1200x800 pixels + pixel digital depth e.g. 8 bit (for
} grayscale -
} 256 gray levels).
} This immediately determines the raw image size as uncompressed
} bitmap.
} E.g. the digital camera images resolution is defined in Megapixels
} defining
} the sensor matrix - which means - pixel X times pixel Y dimentions
of
} image - multiplicated - independent of image ratio (rectangular,
} square
} etc.) This is understandable and clear terminology.
}
} However the "image analysis" people,I am facing now, use the DPI or
} PPI
} parameter as a resolution measurment.
}
} Talking about the shortcut meaning "dots per inch" or "pixels per
} inch" (as
} far as I understand it) - has only the sense when there is a
specific
} media
} described/selected - monitor working at particular grafic card
} resolution,
} scanner sampling with specific optical sensor resolution, hardcopy
} (printout, photo, negative or videoprint) having specific size and
} printing
} head resolution....etc.
} This "inch" must be defined somwhere in physical way.
}
} Some people told me that ANY image in datafile format has own
} RESOLUTION
} defined in this DPI's or PPI's... ?????
} How this should be understood ???
}
} It is for me an absurd until one specifies the physical media where
} this
} "inch" is defined.
}
} PLEASE advise with Yr experience - is that right or not - how the
} resolution
} specified in DPI should be related to image pixel per pixel
} resolution.
}
} kind regards, awaiting clarification....
}
} Krzysztof Herman
} EMISJA - FEI EO Poland
} ul.Ba¿ancia 45A
} 02-892 Warszawa
} tel/fax: (+48 22)6449753, 6449750
} mobile: (+48 601)307456
} kherman-at-labsoft.com.pl
} www.emission.com.pl
}
}
}
}



From daemon Thu May 17 03:47:56 2001



From: Garone, Lynne C :      GARONEL-at-polaroid.com
Date: Thu, 17 May 2001 08:12:05 -0500
Subject: Americium Source

Contents Retrieved from Microscopy Listserver Archives
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We have an Americium x-ray source that we no longer need. My understanding
is that disposal of this is extremely expensive. Has anyone dealt with this
problem and can give me some suggestions?
Thank you,
Lynne C. Garone
Polaroid Corp.
1265 Main St.
W4-1D
Waltham, MA 02451-1714
(781) 386-1446
GaroneL-at-Polaroid.com


From daemon Thu May 17 08:27:17 2001



From: yarrabee-at-caboolture.net.au ()
Date: Thu, 17 May 2001 08:13:29 -0500
Subject: Ask-A-Microscopist: prepare rat liver cells?

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Below is the result of your feedback form. It was submitted by
(yarrabee-at-caboolture.net.au) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 17, 2001 at 04:16:18
---------------------------------------------------------------------------

Email: yarrabee-at-caboolture.net.au
Name: eliza

Organization: QUT

Education: Undergraduate College

Location: brisbane, qld, australia

Question: hello. i am doing an assignment for an EM subject at uni,
and was wondering if anyone could steer me in the right direction. my
question is about which set of methods i would use to prepare rat
liver cells for the examination of mitochondrial ultrastructure.
would it be fair to assume that the usual methods of sample
preparation for TEM could be used in this scenario? that is,
dehydration, fixation and embedding; with the protocols optimised for
mitochondrial preservation? i was also wondering which if any
fixative additives would be appropriate in this case, eg. tannic
acid/potassium ferrocyanide. and whether any immunolocalisation
specific to mitochondria would be applicable to this case. thank you
for your help. eliza.

---------------------------------------------------------------------------


From daemon Thu May 17 08:27:17 2001



From: Nicholas W. M. Ritchie :      nritchie-at-aspexllc.com
Date: Thu, 17 May 2001 08:12:44 -0500
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As the lead software developer for ASPEX's Personal SEM
product, I have had
opportunity to consider this issue. I've come to the conclusion that
the crux of the
problem is sloppy use of language. The word "resolution" is used in
(at least) two
different contexts - resolution to mean "pixel dimensions" (i.e. 1024 x 768) or
resulution meaning "pixel size". Within the "pixel size" meaning
there is additional
ambiguity as to whether "pixel size" refers to the edge length on the
*sample* as
represented by a single pixel or whether "pixel size" refers to the
edge length on the
*display surface* (printer, monitor etc.). Typically image formats
refer to the edge
length of a pixel on the display surface.
Many (but not all) image formats have pixel size (display
surface related) data
encoded with the image. This datum is just a suggestion and usually
(in microscopy)
does not have deep or profound meaning. Usually when the image is
displayed on a video
monitor, the image is displayed by mapping one display pixel to one
image pixel thus
overriding the suggested "pixel size" with the default ~96 to 128 dpi
typical of most
monitors. There is one situation in which the "pixel size" can
represent more than a
suggestion. If the image has a magnification marker burned into the image, the
magnification marker *may* be strictly correct only when the image is
displayed at the
suggested pixel size. By strictly correct I mean that the
magnification is defined
as the scale factor between the length of a pixel edge on the sample
to the length of a
pixel edge on the display surface. Of course, this is not an issue
if you use micron
bars rather than magnification and since digital images are so easily
rescaled I always
recommend the use of micron bars over magnification.

Hope this helps,

Nicholas
###############################
# Nicholas W. M. Ritchie #
# Aspex Instruments #
# 175 Sheffield Drive #
# Delmont, PA 15626 #
# (724) 468-5400 #
# nritchie-at-aspexllc.com #
###############################





5/16/2001 7:21:17 PM, "Krzysztof Herman" {kherman-at-labsoft.com.pl} wrote:

} ------------------------------------------------------------------------
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From daemon Thu May 17 08:29:45 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Thu, 17 May 2001 09:16:04 -0400
Subject: Re: grain size analysis software

Contents Retrieved from Microscopy Listserver Archives
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Becky:

We have a software package (analySIS) from Soft Imaging System in Colorado
that includes grain size analysis per ASTM specs. We use it for both SEM
and LM images and have been happy with it. It allows you to manipulate your
image so the grain boundaries are well defined.

Caveat: I have no involvement with SIS other than being a pleased customer.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
jlagoy-at-bodycote-imt.com



From daemon Thu May 17 09:00:19 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Thu, 17 May 2001 09:54:27 -0400
Subject: Microspheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hello Listers,
} I was wondering if anyone out there can help me out. I am looking
} for a solution of uniform particles (spheres would be nice) of a heavy
} material so that it will show up nicely in backscatter. I also require
that
} the particle size be between 2 and 5 micron. If anyone knows of something
} like this and where I can order it from, It would help me out.
} Thanks in advance
} Nick


From daemon Thu May 17 09:28:54 2001



From: l.tetley :      l.tetley-at-bio.gla.ac.uk
Date: Thu, 17 May 2001 15:21:06 +0100
Subject: Further details RMS EFTEM Meeting, Oxford, July 2001

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers

Details for submission of short Contributed Talks, Registration Form and
Accommodation info for the Oxford EFTEM Meeting are to be found now on the
NEW Royal Microscopical Society website :

http://www.rms.org.uk (+details below) under the EVENTS link.

LT

****************************************************************************
*********
DEVELOPMENTS IN ENERGY-FILTERED ELECTRON MICROSCOPY

A meeting organised by the Royal Microscopical Society
and supported by EMAG, FEI UK Ltd, JEOL (UK) Ltd., LEO EM Inc.,
Gatan UK, TVIPS GmbH

Department of Materials, Oxford University

Wednesday 4 July 2001

REGISTRATION
Online printable form at
http://www.rms.org.uk/current%20events.html#devenergy

CONTRIBUTED TALKS
Contributed presentations are now being sought. Abstracts of no more than
200 words should be sent by email to:
crispin.hetherington-at-materials.ox.ac.uk and should arrive by 31 May, 2001.

OVERVIEW and INVITED SPEAKERS
The use of electron energy filters in analytical electron microscopy
(EFTEM) is a relatively recent development that is proving to be an
extremely powerful tool. In-column filters (e.g. so-called "omega-filters")
and post-column filters (e.g. the "GIF") represent different approaches
which have their own advantages as well as experimental difficulties. This
one day meeting is designed to explore the current state of the art, both
with regard to the instrumentation and also applications of EFTEM in life
and physical sciences.

Dr Bernd Feja (Tietz Video & Image Processing Systems GbmH)
Energy-filtered electron tomography
Prof Joachim Mayer (Aachen University of Technology)
EFTEM - the state of the art and future trends
Dr Paul Midgley (University of Cambridge)
EFTEM image series - taking elemental mapping into a new dimension
Prof Michael Trendelenburg (German Cancer Research Center, DKFZ)
EFTEM in biomedicine & biotechnology: Recent advances in specific
element mapping

Note this meeting is timed to follow immediately the 1-day FEGTEM meeting
to be held on 3 July 2001, also in Oxford.

FURTHER DETAILS
Dr Crispin Hetherington, tel. 01865 273799,
crispin.hetherington-at-materials.ox.ac.uk
Dr Laurence Tetley, tel. 0141 330 4431, l.tetley-at-bio.gla.ac.uk

ACCOMMODATION
Accommodation in Oxford is listed at http://www.oxfordcity.co.uk/accom.
The top page lists the hotels in the £60 and £100 range, but there are
links to many cheaper places. For students (and others?) there is also a
brand new Oxford YHA at 2a Botley Rd, right next to the station. 184 beds
at £18 (B&B).
Tel 01865 762997 or try online reservations on http://www.yha.org.uk.
****************************************************************************
****************
Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel/FAX 0141 330 4431

Integrated Microscopy Facility:
http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


From daemon Thu May 17 09:35:51 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Thu, 17 May 2001 10:30:15 -0400
Subject: Microspheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




}
} Hello Listers,
} I was wondering if anyone out there can help me out. I am looking
} for a solution of uniform particles (spheres would be nice) of a heavy
} material so that it will show up nicely in backscatter. I also require
that
} the particle size be between 2 and 5 micron. If anyone knows of something
} like this and where I can order it from, It would help me out.
} Thanks in advance
} Nick


From daemon Thu May 17 09:36:57 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 17 May 2001 08:23:18 -0600
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krzysztof,

This is indeed a source of confusion. "DPI" or "PPI" makes sense if you want
to print something, as the printers themselves have a given resolution. For
example, if you send something to a printer which has 300 DPI, it is not
very useful to send an image with 1200 DPI. The details don't get printed
and you are wasting space (and possibly bandwidth). So, you are right, that
the medium palys a role for this measure.
The other use of this value comes from scanning (negatives or photos),
because again the resolution is given by the scanner in "DPI".
For any microscopy work or image analysis, the values of "DPI" have little
meaning, as it is the CONTENT of the image that is of interest, not the
MEDIUM. Let's say you take an image in a TEM at 1,000,000x mag. On a ngative
(about 10 cm), you would see roughly 10^-7 m or sample, or 100 nm. If you
take a digital camera and take the same area with a roughly 1Kx1K
resolution, you'd have a resolution of 2.5 x 10^8 DPI, if you wanted to
refer to the real world length coordinates, clearly nonsense.
However, if you printed the image on paper with a print size of 10 cm (4
inch), you would have a resolution of 250 DPI (1000 dots / 4 inch), which is
a value that fits a printer resolution well.

Mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Radostin Danev [mailto:rado-at-nips.ac.jp]
Sent: Wednesday, May 16, 2001 11:44 PM
To: MSA


Dear Krzysztof,

} Some people told me that ANY image in datafile format has own RESOLUTION
} defined in this DPI's or PPI's... ?????
} How this should be understood ???

Some image formats include such information in the file. This allows one to
have an idea about the physical dimensions of the original object.
If only the value of DPI or PPI is specified for an image then it is not
possible to relate it to image pixel resolution.
Both DPI and size of the image in inches should be specified, then:

ImageSizeInPixels = ImageDPI * ImageSizeInInches

Hope this makes sense.

Best regards,

Rado



From daemon Thu May 17 09:46:52 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Thu, 17 May 2001 10:40:55 -0400
Subject: JEOL SEM and Zeiss TEM avail.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists:
}
} Dr. Charles Francis of the Vascular Medicine Unit is listing for sale two
EM
} microscopes and other equipment from an EM lab which will be shutdown this
} summer. The equipment and microscopes are at the University of Rochester
} Medical Center in Rochester, NY (upstate NY).
}
} 1) 1989 JEOL JSM-T330A Digital Scanning EM
}
} 2) 1983 Zeiss EM10C Transmission EM
}
} Also, auxillary equipment, such as:
}
} Water chiller: Coldwell
}
} Reichert TM 60 specimen trimmer
}
} Two Reichert OMUS Ultramicrotomes, purchased in the late 1970's & early
80's
} and still functioning well.
}
} LKB Historange 2218 microtome
}
} If you are interested in these, please e-mail
}
} Administrator, Elizabeth Corrigan: beth_corrigan-at-urmc.rochester.edu
}
} OR
}
} EM technical consultant, Karen Jensen, M.S.:
} karen_jensen-at-urmc.rochester.edu
}




From daemon Thu May 17 10:05:03 2001



From: Doug Cromey :      Cromey-at-Arizona.edu
Date: Thu, 17 May 2001 07:58:07 -0700
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Krzysztof,

I've run into this problem before, its the confusing terminology used by
printers and graphic artists. In a digital microscope image the resolution
is the size of the pixel in microns, everything else is related to output
(screen or paper). See the following links that I took from my web page at
(note that some of the URLs may wrap to a second line):
http://swehsc.pharmacy.arizona.edu/exppath/micro/digimagehardware.html

http://www.kodak.com/US/en/digital/dlc/book3/chapter5/lesson1/p01.shtml
Image Resolution
(Kodak - Printing Digital Images)

http://graphicdesign.about.com/arts/graphicdesign/library/weekly/aa070998.htm
Resolution: DPI, SPI, LPI and PPI
(About.com)

http://www.adobe.com/support/techguides/printpublishing/scanning/psscanning.html
Introduction to Halftones & Scanning
(Adobe)

Best regards.
Doug

At 01:21 AM 5/17/2001 +0200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (NEW email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Thu May 17 10:12:08 2001



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 17 May 2001 08:06:47 -0700
Subject: RE: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Krzysztof Herman writes ...

} However the "image analysis" people, I am facing now, use
} the DPI or PPI parameter as a resolution measurement.
}
} ...
} This "inch" must be defined somewhere in physical way.
}
} Some people told me that ANY image in datafile format has
} own RESOLUTION defined in this DPI's or PPI's... ?????
} How this should be understood ???
}
} It is for me an absurd until one specifies the physical
} media where this "inch" is defined.

I believe the "image analysis" people you refer to speak of a
different context of resolution, different from the definition of
"resolution" which is common to image file formats. This latter
definition is simply a preference for printing which can be changed at
any time. Using your 1200 x 800 example, you could print the image at
6" by 4" at 200ppi, or print it at 12" by 8" at 100ppi ... neither one
of these "print" definitions change the pixel bitmap, rather instruct
the printer where on paper to put the pixel.

"Image analysis" does require a different definition for resolution
which belongs to the object which is imaged ... and this definition
should not change unless the original number of pixels is changed ...
for example, if the original 1200 x 800 is "resampled" to 600 by 400.
Image analysis might want to know the magnification of the image ...
better, know the "real dimension" of a pixel, or the what the distance
between pixel represents in reality. Does it represent 1 micron, 2.6
microns, etc. If this information is known, then image analysis
software can calculate linear measurements or determine areas and
present the information in real terms ... microns, centimeters,
kilometers ... rather than pixels or dots.

As far as I know, no common image format (e.g., TIF) has a place for
defining the "image analysis" context of resolution, only the
"printer" definition. Rather, image analysis software needs to be
instructed of what the real dimensions of a pixel are. There are
softwares which will write "real" resolution definitions to a TIF
file, but is would be uncommon if a different software would know
where to find it. This is the case for my SEM software ... it knows
where to find the magnification definition, but my Image Pro software
does not.

any help? ...
cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Thu May 17 10:36:05 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Thu, 17 May 2001 11:26:46 -0400
Subject: Problems with the Listserver?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

Am I the only one not receiving anything from the listserver. I didn't unsubscribe. I only got stuff from the ICEM folks.

Paula :-(

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Thu May 17 10:45:52 2001



From: Kathy Troughton :      ktrou-at-nb.utmem.EDU
Date: Thu, 17 May 2001 10:40:50 -0500
Subject: LKB Ultrostainer

Contents Retrieved from Microscopy Listserver Archives
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I am using the Leica 2168 Ultrostainer in a cost recovery facility.
Where it cost my users $4.25 per stain run a year ago, I will now have
to charge them $21.00 per run, due to an unbelievable increase in price
and reduction of quantity for supplies.

Does anyone have any suggestions regarding reducing the cost to my
users, such as making stain and refilling bags, other suppliers of
stain, or manufacturers of automatic stainers, etc.

Kathy Troughton
Senior Research Technician
University of Tennessee, Memphis



From daemon Thu May 17 11:01:08 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 17 May 2001 08:53:45 -0700
Subject: Re: LM: Low cost inverted microscopes?

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
If you can get your hands on a Bid Service catalogue, it will usually have a
few inverted light microscopes. Bid Service is a supplier of used
semiconductor manufacturing equipment. Their prices are usually too high,
but they are worth a visit. www.bidservice.com
At 05:27 PM 5/16/01 -0700, you wrote:
}
} Dear All,
}
} A friend is looking for a low cost inverted light microscope (ca $15,000).
} I am not very knowledgeable about what is available so look to you for
} advice.
}
} She was particularly interested in something equivalent to the Zeiss
} Axiovert 100, 135, or even better, the 100TV.
}
} I do know these are no longer being supplied by Zeiss so the names of
} reliable sources for used microscopes would be useful.
}
} All information supplied will remain confidential, although I may post a
} list of useful contacts back onto this forum.
}
}
} Regards,
}
} Paul Webster
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu May 17 11:24:06 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 17 May 2001 17:22:42 +0100
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Krzystof

you will probably get lots of answers to this but here goes:
the value in pixels is a simple quantity (like distance) whereas dpi or
ppi is a rate (like velocity) so they cannot be compared directly unless
you can multiply the rate by inches (or in velocity by time).

You have already said that the importance of dpi is related to a
physical medium. So the two important physical media are source or
object originally captured and the output medium (printer, display
screen etc.). If your 1200 dpi camera can focus close enough to
photograph something 1 inch long then its best resolution would be 1200
dpi or the smallest thing you could see would be 1/1200 inch (approx.
20um). If you use an SEM with digital capture then it's a simple matter
to calculate the digital part of it's resolution for a particular object
which is photographed. The point in both cases is that the resolution
changes because you can get closer or further away. But if you capture
an image using a scanner then the maximum resolution (e.g. 1200 dpi is
fixed).

However if you want to output this to a photographic print you may be
more interested in the maximum size of print you could create. This is
affected by a variety of considerations but the simplest one is to
produce a picture size where the maximum detail can be shown without
loss of resolution. The average human eye at optimum viewing distance
can resolve about 0.2mm so the maximum good print should measure
1200x0.2mm by 800x0.2mm (i.e. 240mm x 160mm or approx. 9 x 6 inches). If
you want to make a bigger picture with maximum resolution then you need
a camera with more pixels, if you just want to show more detail in your
sample you could keep the picture at the same size but photograph the
sample closer.

So, if you are printing, the resolution of your image must be at least
about 150 dpi or for screen output about 75 dpi for the average display
monitor. The number of pixels required would then be dictated by how big
your picture would be on screen or paper. But you might also need to
consider that your audience may want to enlarge the image on screen,
view a print with a magnifier or stand further back to view a display
picture.

This whole situation is made worse, of course, by claimed resolutions of
printers. If it's an inkjet or laser printer then the claim of 1200 dpi
for output is nearer to 20um (or less depending on whose figures you
use) because dithering of several ink spots is required to produce
shades of grey or tones of colour whereas dye sublimation and image
printers usually achieve their actual figure claimed figures of 200 or
300 dpi. So there's no point in creating an image with 400 dpi onto an
inkjet print because the maximum that could be represented by the
printer would be about 200 dpi.

I hope this helps.

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Krzysztof Herman wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Listservers
}
} I am in doubt by one of our customers - specialist in image
} manipulations/publications.
} The talk is about "resolution of an digital image" - in it's virtual form -
} means - in a datafile format - e.g. on floppy.
} According to my understanding - it is described as pixel dimention of an
} image e.g. 1200x800 pixels + pixel digital depth e.g. 8 bit (for grayscale -
} 256 gray levels).
} This immediately determines the raw image size as uncompressed bitmap.
} E.g. the digital camera images resolution is defined in Megapixels defining
} the sensor matrix - which means - pixel X times pixel Y dimentions of
} image - multiplicated - independent of image ratio (rectangular, square
} etc.) This is understandable and clear terminology.
}
} However the "image analysis" people,I am facing now, use the DPI or PPI
} parameter as a resolution measurment.
}
} Talking about the shortcut meaning "dots per inch" or "pixels per inch" (as
} far as I understand it) - has only the sense when there is a specific media
} described/selected - monitor working at particular grafic card resolution,
} scanner sampling with specific optical sensor resolution, hardcopy
} (printout, photo, negative or videoprint) having specific size and printing
} head resolution....etc.
} This "inch" must be defined somwhere in physical way.
}
} Some people told me that ANY image in datafile format has own RESOLUTION
} defined in this DPI's or PPI's... ?????
} How this should be understood ???
}
} It is for me an absurd until one specifies the physical media where this
} "inch" is defined.
}
} PLEASE advise with Yr experience - is that right or not - how the resolution
} specified in DPI should be related to image pixel per pixel resolution.
}
} kind regards, awaiting clarification....
}
} Krzysztof Herman
} EMISJA - FEI EO Poland
} ul.Ba¿ancia 45A
} 02-892 Warszawa
} tel/fax: (+48 22)6449753, 6449750
} mobile: (+48 601)307456
} kherman-at-labsoft.com.pl
} www.emission.com.pl


From daemon Thu May 17 12:53:01 2001



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Thu, 17 May 2001 10:50:24 -0500
Subject: Journal of Em Techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I am trying to locate author information for the Journal of
Electron Microscopy Techniques but I can't find a thing on the web. Does
such a journal exist or is my mind confused? I swear I saw such a journal
once.

Bob
Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
tele: (920) 424-3404
fax: (920) 424-1101
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html


From daemon Thu May 17 12:53:31 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 17 May 2001 12:38:59 -0500
Subject: contract confocal in Dallas area

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I am looking for a confocal microscope in the Dallas TX area that I
could use. I have experience with TEM, SEM, light and standard
fluorescent microscopy, but only minimal experience with confocal.

I am planning on submitting proposals for some immunohistochemistry
work on inner ear tissue where I feel confocal imaging of intact tissue
would yield more information that standard cross section analyses. If
anyone has info on who might have a system in the Dallas area that
would allow outside users or on how much it would cost to use, please
contact me by my direct e-mail.

Thank you,

Karen Pawlowski, Ph.D.
University of Texas at Dallas


From daemon Thu May 17 13:36:35 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 17 May 2001 14:46:44 -0400
Subject: Re: Journal of Em Techniques

Contents Retrieved from Microscopy Listserver Archives
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Nicol -

It seems to me I remember seeing little spheres for sale in the microscopy
supply house catalogues a few years ago. They were made in space, on the
Shuttle, I believe, in zero gravity so were guaranteed to be perfectly
round. I'm not sure of their sizes or composition (they may well be too
light for your purposes). They may even have been sold as "Space
Microspheres" (or "Space Balls?" - or was that a movie?)...They say the
memory is the second thing that goes......

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada

----- Original Message -----
} From: "Nicol Aitken" {nicol-at-semiconductor.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 17, 2001 11:30 AM








I am trying to locate author information for the Journal of
Electron Microscopy Techniques but I can't find a thing on the web. Does
such a journal exist or is my mind confused? I swear I saw such a journal
once.

Bob

Dear Bob,
The journal was renamed some years ago; I think the new name was Microscopy
Research and Technique(s). I think it still exists.
Yours,
Bill Tivol




From daemon Thu May 17 14:19:57 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 17 May 2001 14:12:34 -0500
Subject: Re: Journal of Em Techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The name of the journal was changed to Microscopy Research & Technique.
http://www.interscience.wiley.com/jpages/1059-910X/



}
}
} I am trying to locate author information for the Journal of
} Electron Microscopy Techniques but I can't find a thing on the web. Does
} such a journal exist or is my mind confused? I swear I saw such a journal
} once.
}
} Bob
}
} Dear Bob,
} The journal was renamed some years ago; I think the new name
} was Microscopy
} Research and Technique(s). I think it still exists.
} Yours,
} Bill Tivol

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu May 17 14:30:33 2001



From: DrJohnRuss-at-aol.com
Date: Thu, 17 May 2001 15:26:20 EDT
Subject: re: Journal of Em Techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 5/17/01 2:13:23 PM, wise-at-vaxa.cis.uwosh.edu writes:

} I am trying to locate author information for the Journal of
} Electron Microscopy Techniques but I can't find a thing on the web. Does
} such a journal exist or is my mind confused? I swear I saw such a journal
} once.

It is now Microscopy Research and Technique, very much alive. Contact Dr.John
E. Johnson, Jr., the Editor in Chief, at (650) 366 1644 or email
JEJ-at-Neuroscience.com



From daemon Thu May 17 14:33:58 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 17 May 2001 15:31:19 -0400
Subject: Re: TEM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Prof. Barajon" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Subject: TEM- cell colture fixation and embedding
}
} I need to study the ultrastructural aspects of a cell line grown on petri
} dishes. Any suggestion for the best fixation and embedding protocol (no
} pellets, just fixation and embedding in the dish itself) ?
}
} thanks

I have used Epon substitutes for this with good results. Spurr's resin
reacts with the plastic dish and must be avoided. I fixed, rinsed, osmicated,
rinsed as usual. Dehydration with graded ethanols. Skip the propylene oxide,
it dissolves the dish. Epon will mix with ethanol well enough, I used a
graded series of 2:1, 1:1, 1:2 ethanol:Epon followed by at least 2 changes of
pure Epon. Frequent agitation in all of the steps containing Epon to insure
removal of solvents. Polymerize as usual.
Find an area on the dish you are interested in. Now get a scalpel blade
and heat it in a flame. Use it to cut through the both plastic dish and the
Epon. Often the stress of the cutting will separate the Epon from the dish or
bit of force will separate the two.
Use a file to flatten and roughen the surface of Epon away from the
cells. Glue the small piece of Epon with the cells of interest to a blank
Epon block with "Super Glue". Cut sections. Remember if you are cutting en
face, parallel to the surface the cells were grown on, you will have very
little material to section before you go all the way through your specimen
and hit pure Epon.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu May 17 15:10:06 2001



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Thu, 17 May 2001 12:59:13 -0700
Subject: EM facility Charges response

Contents Retrieved from Microscopy Listserver Archives
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I want to thank everyone who responded to my question about whether user
fees could cover service facility charges. The answer was a resounding
"NO". I received responses from 20 people, 15 of whom were facility
managers across the country. All labs required subsidy from their
University or individual departments. The kind of charges it would take to
cover all costs would be about $200.00 per hour for SEM and $400.00 per
hour for TEM and 60% usage!!. Users object to paying high prices for
services, so business drops off, and the facility shuts down. The burden is
best shared among departments and should involve a combination of sources
including research grants, user fees, and institutional support. At best,
service fees cover 30-50% of costs associated with a facility. This will
give me the necessary information to (hopefully) convince the powers that
be to give us some money. Otherwise, I guess the new EM will have to go in
the garage.

JoAnn Buchanan
Dept. Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856



From daemon Thu May 17 15:20:35 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 17 May 2001 15:01:34 -0500
Subject: Staining parafin sections of pin feathers

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists:

Anyone have a suggestion for staining parafin sections of pin feathers? We
are trying to find melanocytes and keratinocytes.

Thanks
Tim Quinn
Kansas University
Museum of Natural History


From daemon Thu May 17 16:33:17 2001



From: joe fu :      jofu-at-nist.gov
Date: Thu, 17 May 2001 17:27:02 -0400
Subject: Re: Microspheres

Contents Retrieved from Microscopy Listserver Archives
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Frank:
Those space beads (SRM-1960 size of 10um, and SRM-1961 size of 30um) are
latex particles produced by the NASA during the Challenger STS-6 and STS-11
missions, respectively.

At 03:24 PM 5/17/01 -0300, Frank Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov




From daemon Thu May 17 17:00:46 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 17 May 2001 17:53:36 -0500
Subject: Made-in-Space Microspheres

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

F. C. Thomas wrote:
========================================================
It seems to me I remember seeing little spheres for sale in the microscopy
supply house catalogues a few years ago. They were made in space, on the
Shuttle, I believe, in zero gravity so were guaranteed to be perfectly round
. I'm not sure of their sizes or composition (they may well be too light for
your purposes). They may even have been sold as "Space Microspheres" (or
"Space Balls?" - or was that a movie?)...They say the memory is the second
thing that goes......
=========================================================
You have a very good memory.

SPI Supplies placed an order for these made-in-space microspheres (in the
late 1970's) when the program was first announced, and we had the
distinction of being the world's first purchaser of something actually made
in space! I am not sure that got us anything other than a feature one night
on the Nightly News. But despite all the publicity, at the time, that was
given out by NASA, I am unaware of anyone who ever published anything
showing that the "perfectly round" aspects of these microspheres (or any
other aspect for that matter) was anything above and beyond what is
routinely made on earth! We ourselves could not find that superiority, but
perhaps someone better that ourselves did succeed in finding such a
difference.

The last I heard about that was that someone at Lehigh University had some
funding (remember we are talking about the early 1980's) to try to find that
these spheres did, in some way go beyond what could be made on earth, but I
never heard anything after that.

BTW, we subdivided the vial purchased form NASA, and advertised them (as we
were encouraged to do) and over the years, as I met people at our exhibit
booth at trade shows, I never encountered anyone who found, when examining
these spheres, anything "above and beyond" earth made spheres. When the
set of samples was sold off, we never replenished our stock because we could
not in good conscience tell customers that the far higher price could be
justified in terms of there really being something special.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu May 17 17:35:12 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 17 May 2001 15:26:34 -0700
Subject: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

This is a weird question, it could only happen in Santa Cruz.

One of our EH&S people stopped by today to ask if we could analyze some
dust from one of the science buildings on campus. I said 'Maybe' and
listened to the rest of the story.

He told me that a new custodial employee was afraid that the dust in one of
the science buildings was full of toxic material and wanted it tested. The
building is a 30 year old structure that has had various sorts of biology
and chemistry teaching and research labs. As far as I know there has never
been any sort of problem there and EH&S keeps track of things like
radiation and highly toxic situations.

I was reluctant to get involved. Our EDS could probably see some things and
I could recognize some mold spores and cotton fibers, but who am I? I don't
have any training or certification in toxic dust analysis. Plus I probably
already think this guy is a little paranoid about dust, so I probably
wouldn't find anything even if it was there.

Are there labs that can do this sort of analysis and can certify that there
is nothing toxic to worry about in a sample of dust? How does one sample
for these things and how do we respond to this kind of request. What are
the odds that the dust is anything more than just dust? What is dust
anyway?

I promised our EH&S guy that I would ask around among the smartest group of
folks I know. So what do you think?


Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu May 17 17:52:15 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Thu, 17 May 2001 17:50:20 -0500
Subject: Tissue Clear

Contents Retrieved from Microscopy Listserver Archives
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I am posting this for Ricardo Drut. Ricardo wants to know if anyone has
used Tissue -Tek Tissue-Clear as a substite for Xylol, and how do you like
it.
You may respond to Ricardo at his email address. Thanks, Teresa


} ¿Alguien usa este producto como aclarante sustituto del xilol? ¿Con qué
} resultados?

} Ricardo Drut
drut {patologi-at-netverk.com.ar}


From daemon Thu May 17 17:53:26 2001



From: Chao-Ying Ni :      cni-at-udel.edu
Date: Thu, 17 May 2001 17:51:53 -0500
Subject: Consumable supply list for an EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List,

I'm wondering who would like to share a day to day consumable list for a
multiple user EM (both TEM and SEM, and EDS and GIF) lab in materials
science. Idealy, I would like to see a relatively exhaustive but still
common consumable list with both the users (sample prep/imaging) and the
service/maintenance in mind. Thanks in advance!

Chaoying Ni
Director of Electron Microscopy Lab
Dept. of Materials sci. and Eng.
University of Delaware
Newark, DE 19716
cni-at-udel.edu


From daemon Thu May 17 17:54:38 2001



From: Jae Kim :      jkim-at-bnl.gov
Date: Thu, 17 May 2001 17:53:04 -0500
Subject: MgO powde rsample using TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear All:

I would like to get an idea about the way to look at a morphology of a nano
sized powder sample in TEM.

I need to take TEM morphology pictures from MgO powder (from nano meter to
a few micron size distribution) using a Cu grid. To spread a MgO particle
on the grid, I will spray some kinds of alcohol on the powder which resides
on the grid. Here, I would like to know what kinds of alcohol can be used
to get a clear image without melting the holey carbon film on the grid.

I appreciate your comments.

Jae

********************************
Jae-yong Kim
Post-doctoral Research Associate
Chemistry Department
Brookhaven National Laboratory
Upton, NY 11973
Tel:631-344-4317
Fax:631-344-5815
********************************


From daemon Thu May 17 19:32:19 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Thursday, May 17, 2001 4:53 PM
Subject: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
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Dear Jon and all;

Is the dust toxic?
Interesting question from the technophobic world. Beyond looking for the
obvious heavy metals and suspicious asbestos like fibers, a microscopist/EDS
analyst would have a hard time answering that question.
You would have to treat the dust like a new drug to answer the question.
Feed large quantities to mice and stick it into rabbit eyeballs to look for
toxic symptoms and death. But don’t let the animal rights people know that
you are doing such torture to animals. It is better that people get sick
than to make animals suffer. UC Santa Cruz probably has a policy against
such testing anyway.

Ron Vane
XEI Scientific


-----Original Message-----
} From: Jon Krupp {jmkrupp-at-cats.ucsc.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Thu May 17 20:15:21 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 18 May 2001 11:11:45 +1000
Subject: RE: Microspheres

Contents Retrieved from Microscopy Listserver Archives
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Your particle size is a bit large for "colloidal" gold. How about magnetite
spheres - as described below? Certainly they should show up in backscatter. My
only concern are the on/off magnetic properties. I guess that an SEM (or
optical scope!) would not be affected, but in TEM, with the powerful objective
lens right next to the particles, there would be a problem. Those particles are
available in graded and defined sizes from 1 to 8um.
Disclaimer: ProSciTech is a world-wide distributor for Spherotech, but it makes
little sense for US user to come to us. I suggest that you check out the
spherotech.com site.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

PARAMAGNETIC PARTICLES - MAGNETIC PARTICLES
The magnetic particles are prepared according to the procedures described in US
Patent No. 5,091,206. They are prepared by coating a layer of magnetite and
polystyrene onto monodispersed (ie. uniform sized) polystyrene core particles.
As a result, the magnetic particles are spherical in shape, and paramagnetic in
nature. They are also very uniform in size. The magnetite contents of these
magnetic particles can be adjusted but in general it represents about 10% to
15%. The magnetic particles can be easily separated from a suspension
magnetically. These particles become non-magnetic when removed from a magnet,
and do not retain any detectable magnetism even after repeated exposure to
strong magnetic field. The magnetic particles can be used for cell separation,
affinity purification, DNA probe assays, magnetic particle EIA, etc.


On Thursday, May 17, 2001 11:54 PM, Nicol Aitken [SMTP:nicol-at-semiconductor.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} }
} } Hello Listers,
} } I was wondering if anyone out there can help me out. I am looking
} } for a solution of uniform particles (spheres would be nice) of a heavy
} } material so that it will show up nicely in backscatter. I also require
} that
} } the particle size be between 2 and 5 micron. If anyone knows of something
} } like this and where I can order it from, It would help me out.
} } Thanks in advance
} } Nick


From daemon Thu May 17 23:34:55 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 17 May 2001 23:29:37 -0500
Subject: RE: Microspheres

Contents Retrieved from Microscopy Listserver Archives
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Those were sold by NIST, I believe, and were latex spheres. Latex spheres
are still available in a variety of sizes, but the special property of the
space grown variety was the small standard deviation of size resulting from
their microgravity production. There was only one or two batches made,
prior to the Challenger incident, and all were probably sold long ago.

Latex would not be a good choice, obviously, but there are gold
microspheres made for antibody research that may work. I, unfortunately,
don't know what sizes they are produced in or the distributors who might
have them off the top of my head. However, pump a search engine with
"colloidal gold" as a search phrase and you should find them all.

On Thursday, May 17, 2001 1:24 PM, Frank Thomas
[SMTP:thomasf-at-AGC.BIO.NS.CA] wrote:
}
} Nicol -
}
} It seems to me I remember seeing little spheres for sale in the
microscopy
} supply house catalogues a few years ago. They were made in space, on the
} Shuttle, I believe, in zero gravity so were guaranteed to be perfectly
} round. I'm not sure of their sizes or composition (they may well be too
} light for your purposes). They may even have been sold as "Space
} Microspheres" (or "Space Balls?" - or was that a movie?)...They say the
} memory is the second thing that goes......
}
} F.C. Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada
}
} ----- Original Message -----
} } From: "Nicol Aitken" {nicol-at-semiconductor.com}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, May 17, 2001 11:30 AM
} Subject: Microspheres
}
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------
---.
} }
} }
} }
} }
} } }
} } } Hello Listers,
} } } I was wondering if anyone out there can help me out. I am looking
} } } for a solution of uniform particles (spheres would be nice) of a
heavy
} } } material so that it will show up nicely in backscatter. I also
require
} } that
} } } the particle size be between 2 and 5 micron. If anyone knows of
} something
} } } like this and where I can order it from, It would help me out.
} } } Thanks in advance
} } } Nick
} }
} }
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Fri May 18 00:44:05 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 18 May 2001 00:35:23 -0500
Subject: RE: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You think the question is strange? Be careful what you look for - you may
find more than you want.

Most probably missed it, but ABC recently did a piece in their national
network news about St. Charles East High School, the school my younger
daughter is a junior in. For years there have been complaints of air
problems in the school related to symptoms of allergic problems of all
kinds. The school has tried numerous labs to try to understand what may be
the cause. Well and good, my own daughter has had problems that we can
directly link to her time in that school, and I'd like to know why.

During spring break this year, they brought in one company from out of
state. This company discovered several molds growing behind the walls that
caused the school district to just shut the whole school down. The
students (over 3000 of them), after a few weeks off, are now time sharing
facilities with a second high school in town while the school board decides
if they will have to tear the buildings down or just remodel.

In the last week, the same molds were found infesting a number of buildings
and homes in Texas. The buildings have been condemned until the problem is
taken care of and several home owners are finding that it will cost them
more than $50,000 just to get back into their houses.

Stachybotris(?) is the culprit. There are few studies on its effects that
I am aware of, but the common assumption is that it is dangerous, if not
deadly. I recall something around a year ago that pointed to the
possibility of potentially serious effects on infants exposed to it. Yet
the current student population had an average of 6000 student years of
possible exposure without a serious link, but they can now not be exposed
for a second more. In fact, after weeks of debate, the school reluctantly
allowed parents in for a limited time to collect anything left in school
and gym lockers, after signing legal waivers and being checked in and out
(I did not appreciate collecting gym clothes after weeks of fermentation).

On a similar vein, in the Chicago area, there has been considerable
sensitivity to mercury. Several contractors hired by the local electrical
utility apparently screwed up the replacement of mercury containing usage
meters. It got to the point where the utility was doing forced testing of
any home that may have been affected - if any trace of mercury was found,
expensive remediation efforts were required before the homeowners were
allowed back in.

One poor dope, in this time frame, uncertain what to do after breaking a
mercury thermometer at his place of work, decided to call the local fire
department. The building was immediately shutdown and quarantined by the
EPA and the business had to wait, and pay for, space-suited remediation
teams to clear all traces of mercury from the building.

In the case of both contaminants, potential levels of exposure were not a
consideration. Simply finding one of these contaminants resulted in the
unrealistic total quarantine response. I'm sure that these responses are
not isolated in today's litigious society, just not documented as a whole.

Any building built 30 - 40 years ago was probably of a certain 'energy
efficient' design of the time. In other words, windows don't open and
fresh air don't get in. While reducing the loss of heated or cooled air,
and thus producing less of a load on HVAC designs, these buildings
essentially ensure that anything entering their environment will stay
there. That includes moisture, which promotes bacterial, mold and fungus
growth, as well as any material introduced. 'Sick Building Syndrome' is
now a classified problem that covers more than you can imagine - including
the release of gaseous compounds from new carpeting, upholstery, wood and
plastic materials as well as any seemingly minor release of any solid,
liquid or gaseous material that ever happened in the building.

Take my advice for what you paid - let the EH&S people find their own
resources. This seemingly simple request could end up in a major political
pain in the ass in your institution. If EH&S finds a problem like the
above, then they can take cover in the fact that they were just doing their
job. If you are responsible for finding it, you may not have that kind of
protection. Rest assured, if a major problem ensues, EH&S will be more
than happy to pass the credit to you.

On Thursday, May 17, 2001 5:27 PM, Jon Krupp [SMTP:jmkrupp-at-cats.ucsc.edu]
wrote:
}
} Hi:
}
} This is a weird question, it could only happen in Santa Cruz.
}
} One of our EH&S people stopped by today to ask if we could analyze some
} dust from one of the science buildings on campus. I said 'Maybe' and
} listened to the rest of the story.
}
} He told me that a new custodial employee was afraid that the dust in one
of
} the science buildings was full of toxic material and wanted it tested.
The
} building is a 30 year old structure that has had various sorts of biology
} and chemistry teaching and research labs. As far as I know there has
never
} been any sort of problem there and EH&S keeps track of things like
} radiation and highly toxic situations.
}
} I was reluctant to get involved. Our EDS could probably see some things
and
} I could recognize some mold spores and cotton fibers, but who am I? I
don't
} have any training or certification in toxic dust analysis. Plus I
probably
} already think this guy is a little paranoid about dust, so I probably
} wouldn't find anything even if it was there.
}
} Are there labs that can do this sort of analysis and can certify that
there
} is nothing toxic to worry about in a sample of dust? How does one sample
} for these things and how do we respond to this kind of request. What are
} the odds that the dust is anything more than just dust? What is dust
} anyway?
}
} I promised our EH&S guy that I would ask around among the smartest group
of
} folks I know. So what do you think?
}
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Fri May 18 03:17:11 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 18 May 2001 09:13:44 +0100
Subject: Re: image resolution...

Contents Retrieved from Microscopy Listserver Archives
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Whoops in my last mailing my spell checker helped me to send an error in
the last paragraph about printers. The 20 um should have been 200 dpi in
the sixth line below:-

} Krzystof
} you will probably get lots of answers to this but here goes:
} {SNIP}
} This whole situation is made worse, of course, by claimed resolutions of
} printers. If it's an inkjet or laser printer then the claim of 1200 dpi
} for output is nearer to 20um (or less depending on whose figures you
} use) because dithering of several ink spots is required to produce
} shades of grey or tones of colour whereas dye sublimation and image
} printers usually achieve their actual figure claimed figures of 200 or
} 300 dpi. So there's no point in creating an image with 400 dpi onto an
} inkjet print because the maximum that could be represented by the
} printer would be about 200 dpi.

I guess it just shows how easy it is to mix up pixel size and dpi.

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK


From daemon Fri May 18 03:59:35 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 18 May 2001 09:55:13 +0100 (GMT Daylight Time)
Subject: Re: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would admit I am not an expert and collect a quick EDX
spectrum for heavy metals and have a look for asbestos
fibres.

Dave


On Thu, 17 May 2001 15:26:34 -0700 Jon Krupp
{jmkrupp-at-cats.ucsc.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} This is a weird question, it could only happen in Santa Cruz.
}
} One of our EH&S people stopped by today to ask if we could analyze some
} dust from one of the science buildings on campus. I said 'Maybe' and
} listened to the rest of the story.
}
} He told me that a new custodial employee was afraid that the dust in one of
} the science buildings was full of toxic material and wanted it tested. The
} building is a 30 year old structure that has had various sorts of biology
} and chemistry teaching and research labs. As far as I know there has never
} been any sort of problem there and EH&S keeps track of things like
} radiation and highly toxic situations.
}
} I was reluctant to get involved. Our EDS could probably see some things and
} I could recognize some mold spores and cotton fibers, but who am I? I don't
} have any training or certification in toxic dust analysis. Plus I probably
} already think this guy is a little paranoid about dust, so I probably
} wouldn't find anything even if it was there.
}
} Are there labs that can do this sort of analysis and can certify that there
} is nothing toxic to worry about in a sample of dust? How does one sample
} for these things and how do we respond to this kind of request. What are
} the odds that the dust is anything more than just dust? What is dust
} anyway?
}
} I promised our EH&S guy that I would ask around among the smartest group of
} folks I know. So what do you think?
}
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri May 18 04:00:31 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 18 May 2001 09:56:57 +0100 (GMT Daylight Time)
Subject: Re: MgO powde rsample using TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used ethanol.


Dave


On Thu, 17 May 2001 17:53:04 -0500 Jae Kim {jkim-at-bnl.gov}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear All:
}
} I would like to get an idea about the way to look at a morphology of a nano
} sized powder sample in TEM.
}
} I need to take TEM morphology pictures from MgO powder (from nano meter to
} a few micron size distribution) using a Cu grid. To spread a MgO particle
} on the grid, I will spray some kinds of alcohol on the powder which resides
} on the grid. Here, I would like to know what kinds of alcohol can be used
} to get a clear image without melting the holey carbon film on the grid.
}
} I appreciate your comments.
}
} Jae
}
} ********************************
} Jae-yong Kim
} Post-doctoral Research Associate
} Chemistry Department
} Brookhaven National Laboratory
} Upton, NY 11973
} Tel:631-344-4317
} Fax:631-344-5815
} ********************************
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri May 18 05:25:55 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 18 May 2001 11:24:33 +0100
Subject: Re: Microspheres

Contents Retrieved from Microscopy Listserver Archives
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Nicol

I can't think of any electron dense spheres of the size that you
suggest. The nearest I have seen is approx. 1um diameter latex spheres
which are probably available from most suppliers. If you want electron
density you could perhaps consider fixing some in a 1 or 2% solution of
osmium tetroxide for 1 hour or more. You could then wash and examine
them to see if they have enough density, retain size and shape. An
alternative might be to gold sputter coat latex beads but they would
have to be dried down then resuspended and the gold might wash off. I
haven't tried either of these but if you use them and they work I would
love to know.

Malcolm

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Nicol Aitken wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} }
} } Hello Listers,
} } I was wondering if anyone out there can help me out. I am looking
} } for a solution of uniform particles (spheres would be nice) of a heavy
} } material so that it will show up nicely in backscatter. I also require
} that
} } the particle size be between 2 and 5 micron. If anyone knows of something
} } like this and where I can order it from, It would help me out.
} } Thanks in advance
} } Nick


From daemon Fri May 18 06:01:44 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 18 May 2001 09:53:08 -0300
Subject: Re: LaB6 problem

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Jon -

Although not accredited to do this kind of work "officially", I once put
some dust collected from air filters in a building in the SEM. A friend of
mine was the enivironment manager for a local communications company, and
they were concerned about toxins in the workplace. The mold "Stachybotris"
was one thing we were looking for (but didn't find). We also found no
aluminosilicate fibres which could have been asbestos. We did find clay
particles, combustion products, lots of little "organic" fibres (wool?
polyester? cotton?) and assorted bits of micro-crud (that's a technical
term). EDS analysis of some bits showed fair amounts of titanium, which I
understand is a common ingredient in paints.
So there wasn't anything obviously very worrisome in there.
But never underestimate the power of Stachybotris - we had a large part
of the Institute here shut down and then rebuilt a couple years ago because
of it. Cost God knows how much, and people here are still concerned if
someone finds a black stain on anything that could be damp.
Anyway, dust analysis can be a pretty interesting exercise - I wish I had a
copy of the McCrone Particle Atlas - it probably would have helped a lot.

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Dartmouth, Nova Scotia
Canada

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 17, 2001 7:26 PM


Listers -

Those of you with LaB6 experience - is it possible for one to fail in such a
way that it still shows a "normal" emission current on all the gauges, yet
is not producing a findable beam?
Last Monday we stripped our ESEM down for a bi-annual cleaning, inspected
everything, including the LaB6 (which looked fine, and had been working well
up to that point), put the whole thing back together, and were unable to
find a beam. Now we've done this any number of times in the last 8 years,
and it's never more than a few minutes to find the beam afterwards. Turn the
condensor down low, select a 3mm "hole" instead of a projection aperture,
and bingo, there it is - a big, shiny, beam like a floodlight.
This time, nothing. Not a glimmer of "light" on the monitor, even though the
LaB6 is "on", with 15KeV, and a "normal" emission current. We've had the
column back apart 4 times now, to see if there's some physical blockage
somewhere which might prevent the beam from reaching the chamber, but
everything looks great. Even checked the performance of the chamber
isolation valve, in case it was failing to open, but it's working properly.
So I'm left with thinking maybe it's the LaB6, but in the past when we had
one fail, it either suddenly began producing a strange, assymetrical beam,
with normal emission current readings, or no beam at all, with no emission
current showing. What gives? We have a new LaB6 on order, as a spare, so
when it arrives I'll put it in, unless somebody has any other ideas....

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Fri May 18 08:00:13 2001



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 18 May 2001 07:53:44 -0500
Subject: Re: image resolution...

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Did anyone mention that in the video world, "resolution"
has long meant half of the value as used by computer people?

Television technicians (or composite video monitor specs, etc.)
will still speak of "resolution" as in "lines of resolution"
as in the number of vertical black lines that can be discerned
apart from a white background.

Is this true for print photography, too?

- John



From daemon Fri May 18 08:00:13 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 18 May 2001 13:56:44 +0100
Subject: MIcrospheres, another use for

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Hello all Listers,

Some time ago, someone in our chemistry department made a aqueous
suspension of silica microspheres, about half a micron in diameter. If
this was allowed to dry out, the spheres formed a close-packed lattice,
similar to that in opal which gives rise to the lovely diffraction
colours. When examining this lattice under the SEM, we found that this
made an ideal target for the AUTO aSTIGmatism control, so when examining
something bland such as a fine textured plastic surface, one could use a
clump of these spheres as a target and then have the optics just right
for examining the bland specimen.

Alas, the suspension of spheres has degenerated into some sort of "goo",
and no more is available. Are there any other sources of spheres of
this size? The 1 to 8 micron space spheres are probably a little too
big, and I am a bit wary of what magnetite spheres might do in the
electric field of the SEM.

+----------------------------------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+----------------------------------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+----------------------------------------------------------------+




From daemon Fri May 18 09:00:49 2001



From: bryan.tracy-at-amd.com
Date: Fri, 18 May 2001 06:49:19 -0700
Subject: Double Tilt Holder for Topcon 002B TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

we are semi-desperately looking for a Gatan double-tilt holder
for our Topcon 002B TEM. We are using the 1.9A polepiece.

Prompt payment - if interested please contact:

bryan.tracy-at-amd.com
408-749-4819

many thanks!!









From daemon Fri May 18 09:13:12 2001



From: Scott Wight :      scott.wight-at-nist.gov
Date: Fri, 18 May 2001 10:09:30 -0400
Subject: Re: Made-in-Space Microspheres

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I wasn't involved in the NIST (NBS then - before my time) work but a quick
search of NASA web brought up this report and many more on the microspheres:
http://samson2.msfc.nasa.gov/fame/exps/van-011.html
it contains references to journal articles for those interested in more
information. SRM 1960 and 1961 are listed as available as Standard
Reference Materials at http://srmcatalog.nist.gov/
Scott


} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} }
} SPI Supplies placed an order for these made-in-space microspheres (in the
} late 1970's) when the program was first announced, and we had the
} distinction of being the world's first purchaser of something actually made
} in space! I am not sure that got us anything other than a feature one night
} on the Nightly News. But despite all the publicity, at the time, that was
} given out by NASA, I am unaware of anyone who ever published anything
} showing that the "perfectly round" aspects of these microspheres (or any
} other aspect for that matter) was anything above and beyond what is
} routinely made on earth! We ourselves could not find that superiority, but
} perhaps someone better that ourselves did succeed in finding such a
} difference.
}
} The last I heard about that was that someone at Lehigh University had some
} funding (remember we are talking about the early 1980's) to try to find that
} these spheres did, in some way go beyond what could be made on earth, but I
} never heard anything after that.
}
} BTW, we subdivided the vial purchased form NASA, and advertised them (as we
} were encouraged to do) and over the years, as I met people at our exhibit
} booth at trade shows, I never encountered anyone who found, when examining
} these spheres, anything "above and beyond" earth made spheres. When the
} set of samples was sold off, we never replenished our stock because we could
} not in good conscience tell customers that the far higher price could be
} justified in terms of there really being something special.
}

------------------------------------------------------------------
Scott Wight fax: 301-417-1321
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | email: scott.wight-at-nist.gov
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Fri May 18 09:16:30 2001



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 18 May 2001 09:23:10 -0500
Subject: Re: TEM Question

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I will just add my personal variations to the general method
described by Geoff McAuliff:

Fjx & dehydrate (ethanols) as usual.

I have found that the following "hybrid" Epon analog separates
cleanly from the culture dish just about every time. I Use LLX112
and DMP-3o, both from Ladd, and DDSA and NMA from Electron Microscopy
Sciences. I have no clue why this particular mix works best, but a
number of years ago, I got my hands on just about every component
from every manufacturer and found that this mix did not react with
the dishes at all, while many of the others did to some extent. I'd
previously assumed that these components were all manufactured by one
lab and just sold by the various vendors under their individual
names, but the LX1l12 looks different than the other Epon812
substitutes.

The other thing that I do differently is in the actual embedding. I
put a thin layer of the mixed resin into the dish (2 mm?), and insert
tubes (made by cutting the pyramidal ends off of BEEM capsules)over
areas of interest. I partially polymerize this (overnight) and in
the morning, I fill JUST THE TUBES the rest of the way with more
resin and then fully polymerize it all. When Its "cooked" I take a
pair of needlle-nosed pliers, grab a tube and break it out with a
slight twist to apply shearing force. It comes out cleanly.
sometimes a small bit of the dish will come with it, but that pops
right off when you start to trim the block. I just took a batch out
of the oven this morning. I have nice clean faces with the cells
clearly visible.

good luck,
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri May 18 09:19:58 2001



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Fri, 18 May 2001 09:17:54 -0500
Subject: Tissue Clear

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I am posting this for Ricardo Drut. Ricardo wants to know if anyone has
used Tissue -Tek Tissue-Clear as a substite for Xylol, and how do you like
it.
You may respond to Ricardo at his email address. Thanks, Teresa


} ¿Alguien usa este producto como aclarante sustituto del xilol? ¿Con qué
} resultados?

} Ricardo Drut
drut {patologi-at-netverk.com.ar}


From daemon Fri May 18 09:20:45 2001



From: Lenn C Kupferberg :      Lenn_C_Kupferberg-at-raytheon.com
Date: Fri, 18 May 2001 09:18:45 -0500
Subject: Americium Source

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You might try talking with Jim McCarthy. Jim is part of our EH&S
group in our Sudbury Lab. Jim has had experience disposing of radioactive
sources. Jim's contact information follows.

Lenn C. Kupferberg
Principal Physicist
Raytheon Company
Electronic Systems
Lexington
Laboratory
131 Spring Street
Lexington, MA
02421-7803
(781)860-3082


James J. McCarthy
Raytheon Company
C3I
Sudbury Laboratory
528 Boston Post Road
Sudbury, Mass. 01776
(978)440-4047
James_J_McCarthy-at-raytheon.com

---------------------- Forwarded by Lenn C Kupferberg/RES/Raytheon/US on
05/18/2001 08:09 AM ---------------------------


Norman C Miller
05/17/2001 06:02 PM

To: Lenn C Kupferberg/RES/Raytheon/US-at-MAIL
cc:


We have an Americium x-ray source that we no longer need. My understanding
is that disposal of this is extremely expensive. Has anyone dealt with
this
problem and can give me some suggestions?
Thank you,
Lynne C. Garone
Polaroid Corp.
1265 Main St.
W4-1D
Waltham, MA 02451-1714
(781) 386-1446
GaroneL-at-Polaroid.com


From daemon Fri May 18 09:22:21 2001



From: Chao-Ying Ni :      cni-at-udel.edu
Date: Fri, 18 May 2001 10:18:14 -0400 (EDT)
Subject: Philips 400T available

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Dear colleagues,

We have a Philips 400T for sell. It had been kept on contract. And the
whole system, including EDS and STEM, has been in full function before it
was moved out of the lab and packed to have space for a 200kV scope.
Anybody interested in purchasing please contact me off line. Thanks

Chaoying Ni
Director of Electron Microscopy Lab
Dept. of Materials sci. and Eng.
University of Delaware
Newark, DE 19716
cni-at-udel.edu



From daemon Fri May 18 09:41:35 2001



From: Chao-Ying Ni :      cni-at-udel.edu
Date: Fri, 18 May 2001 10:36:45 -0400 (EDT)
Subject: EM Lab Server OS: Linux or Windows 2000?

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Dear colleagues,

I'm going to build an EM lab server to run web and other applications,
to share files, especially to have an interactive wed scheduling software
to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I have
narrow down the platform to Linux and Windows 2000. Could you please give
me some inputs concerning the pros and cons of these two operating
systems? Any comments and suggestions on the hardware selection are also
more than welcome. Thanks in advance!

Chaoying Ni
Electron Microscopy Lab
Dept. of Materials sci. and Eng.
University of Delaware
Newark, DE 19716
cni-at-udel.edu




From daemon Fri May 18 09:51:50 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 18 May 2001 10:45:15 -0400
Subject: Re: Dust analysis?

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}
} This is a weird question, it could only happen in Santa Cruz.
}
} One of our EH&S people stopped by today to ask if we could analyze some
} dust from one of the science buildings on campus. I said 'Maybe' and
} listened to the rest of the story.
}

I have several times been asked by our Environmental Medical people to
analyze specific samples of deposits found in various places around the
campus, usually finding them to be quite benign (steel filings, plaster
dust, (presumably from sheetrock), soap deposits, etc. etc). The steel and
plaster I did with the SEM, other things (like the soap) I passed to other
members of our team here for techniques like FTIR.

Taking a generalized "dust" sample and proving beyond doubt that it is
harmless is a totally different issue.

Dust, of course, is simply whatever happens to fall on the floor that is
too small to be picked up piece-by-piece. Dust in the bedroom at home is
very different from dust in a laboratory. In some cases it will be
dominated by biological material (human skin and hair, and fibers from
clothing). In other cases by residue from construction activities in other
parts of a building. Depending on the ventilation arrangements,
particulate matter from outside may be brought in. This could contain
soots from internal combustion engines or oil or coal fired electric
plants, or even wildfires, or fine mineral particles (sometimes from very
remote sources), as well, possibly, as aerosols or other particulates from
local industrial activity. There will always be material brought in on
shoes, especially in the winter, and, of course, wear debris from traffic
in the floor.

Can a non-specialist determine whether it is "hazardous"? (What is
"hazardous", anyway?) Not in my book. There is just too much to look for,
especially if you are trying to reassure a paranoid and suspicious person.
One example - does finding Si and Al in a particle tell you that it is
asbestos? Of course not. Does a failure to find Si and Al mean asbestos
is absent? Again, of course not - it could simply be a minor constituent,
and the elemnts below the detectability limit.

Good luck!

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri May 18 10:12:42 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 18 May 2001 08:13:49 -0700
Subject: Re: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
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I have done bunches of SEM shots of house dust. Really
quite interesting. This was not a search for a smoking gun,
just an interesting exercise. I found (all very small) fibers,
skin flakes, odds and ends of all other sorts of things. There
we no mites or other live (before SEM) or dead organisms
that I could find. The specimens were taken on a sticky
stub sitting on top of a TV so the static electricity would
draw room junk to the stub. It did.

I have several other ones at floor level which I have not
yet imaged. I usually wait about 4 months before coating
and imaging. This guarantees a good smattering of
crud.

Old buildings would have, I think, Aspergillis mold. This
would explain respiratory problems and many ills. There
are other mold types of course.

gary g.


At 03:26 PM 5/17/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri May 18 10:41:16 2001



From: Menconi, Michael,Ph.D :      MMENCONI-at-PARTNERS.ORG
Date: Fri, 18 May 2001 11:36:38 -0400
Subject: Mitochondria, MPT

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I am interested in studying mitochondrial ultrastructure in various tissues
during hypoperfusion and was wondering if there were any specific TEM
stains/techniques or immunofluorescent techniques for examining the
mitochondrial permeability transition? Thanks.


Michael J. Menconi, Ph.D.

Department of Surgery
Brigham and Women's Hospital
Medical Research Building, Room 502
75 Francis Street
Boston, MA 02115
Phone: 617-278-0693
FAX: 617-582-6047
Email: mmenconi-at-partners.org



From daemon Fri May 18 10:58:27 2001



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Fri, 18 May 2001 08:54:20 -0700 (PDT)
Subject: Re: Microspheres

Contents Retrieved from Microscopy Listserver Archives
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Nick - Check out Duke Scientific (dukescientific.com) who sell a range of
different size microspheres for calibration purposes. Many are either
glass, silica or polystyrene which won't specifically meet your high Z
requirement, however, you could easily coat them with Au or Pd or some
other metal.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195
joswiak-at-astro.washington.edu
(206)543-7702


On Thu, 17 May 2001, Nicol Aitken wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} }
} } Hello Listers,
} } I was wondering if anyone out there can help me out. I am looking
} } for a solution of uniform particles (spheres would be nice) of a heavy
} } material so that it will show up nicely in backscatter. I also require
} that
} } the particle size be between 2 and 5 micron. If anyone knows of something
} } like this and where I can order it from, It would help me out.
} } Thanks in advance
} } Nick
}
}



From daemon Fri May 18 12:49:40 2001



From: christine :      ac.richardson2-at-btinternet.com
Date: Fri, 18 May 2001 19:18:08 +0100
Subject: Proto-Slo

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Prof. Barajon, take a look in our paper entitled "SANTOS & MARIATH, 1997. A
simple method for fixing, dehydrating and embedding pollen tubes cultivated
in vitro for optical and transmission electron microscopy. Biotechnic &
Histochemistry, 72(6): 315-319" . Could be your problem solution!
Hoping success!
Mariath

----- Original Message -----
} From: "Geoff McAuliffe" {mcauliff-at-umdnj.edu}
To: "Prof. Barajon" {isabella.barajon-at-unimi.it}
Cc: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 17, 2001 4:31 PM



Dear Paul,
I seem to remember using a 3% solution of methyl cellulose to slow down
paramecium.I suppose the concentration can be adusted for larger beasties.

Christine Richardson
E.M.Unit
University of Durham
Dept.Biological Science

Durham
England
-----Original Message-----
} From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, May 15, 2001 9:46 PM
To: microscopy-at-sparc5.microscopy.com


Dear Listers,

I have some 5-to-8 year-olds who really enjoy the microworld, but whose
hands
can't keep up with speedy microbeasties under the lens. I remember from
years past that there was a product (I think it was called 'Proto-Slo', or
similar) that was viscous, roughly iso-osmotic, and designed to slow the
critters down for easier observation. Is it still available? If not, does
anyone have a recipe for a home-brewed equivalent?

Thank you, thank you, thank you,

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN




From daemon Fri May 18 13:30:50 2001



From: Stan Gelles :      sgelles-at-cctlabs.com
Date: Fri, 18 May 2001 14:26:59 -0400
Subject: Peak Instrument Co. WDS System

Contents Retrieved from Microscopy Listserver Archives
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I am trying to find out whether there is anyone or organization that is
available to service a Peak Instrument Co. Focus MCS 4 Multi Element Crystal
Spectrometer Model O,C,N,B integrated with a PGT Imix System.

Thanks for your help.

Stanley H. Gelles
Senior Group Leader
CC Technologies
614-761-1214
FAX 614-761-1633



From daemon Fri May 18 13:45:35 2001



From: christine :      ac.richardson2-at-btinternet.com
Date: Fri, 18 May 2001 19:39:39 +0100
Subject: re:Proto-slo

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
I seem to remember using a 3% solution of methyl cellulose to slow down
paramecium.I suppose the concentration can be adusted for larger beasties.

Christine Richardson
E.M.Unit
University of Durham
Dept.Biological Science
Durham
England


From daemon Fri May 18 13:46:00 2001



From: christine :      ac.richardson2-at-btinternet.com
Date: Fri, 18 May 2001 19:40:44 +0100
Subject: Proto-Slo

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
I seem to remember using a 3% solution of methyl cellulose to slow down
paramecium.I suppose the concentration can be adusted for larger beasties.

Christine Richardson
E.M.Unit
University of Durham
Dept.Biological Science

Durham
England
-----Original Message-----
} From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, May 15, 2001 9:46 PM
To: microscopy-at-sparc5.microscopy.com


Dear Listers,

I have some 5-to-8 year-olds who really enjoy the microworld, but whose
hands
can't keep up with speedy microbeasties under the lens. I remember from
years past that there was a product (I think it was called 'Proto-Slo', or
similar) that was viscous, roughly iso-osmotic, and designed to slow the
critters down for easier observation. Is it still available? If not, does
anyone have a recipe for a home-brewed equivalent?

Thank you, thank you, thank you,

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN




From daemon Fri May 18 14:19:08 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 18 May 2001 12:37:56 -0700
Subject: Re: LaB6 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Frank,

Bad luck indeed. It would be helpful to know what make of LaB6 you are
using because the physical construction may have a great deal to do with the
diagnosis of your problem. Anyway, what I suspect, given the eight years of
experience with your instrument is that nothing incorrect was done on your
part but rather, the LaB6 itself has gone bad. If you have not done so yet,
it might be helpful to recenter the tip and while you have everything out,
take a very close look at the crystal itself. They have been known to
seperate from their heater block and thermal shock can cause the crystal to
crack, causing mechanical displacement. The crystal may have actually
fallen off the block and all you are doing is heating the carbon heater. If
you can, it would eliminate a bunch of variable if you would install a plain
old hair-pin Tungsten Filament and see how the instrument behaves.

Good luck.


Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone 512/282-5507 FAX 512/280-0702

Sustaining Member - MICROSCOPY SOCIETY OF AMERICA
----- Original Message -----
} From: "Frank Thomas" {thomasf-at-AGC.BIO.NS.CA}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, May 18, 2001 7:53 AM


If your column has a column liner and an aperture at the
bottom of the liner, could it have gotten some crud on it
during your work on the system? I would think that with
this aperture blocked, there would not be any emission
current.

What about carefully checking the SE detector? Faraday
shield (make sure it is in-place correctly), check the
scintillator disc, etc. It sounds like the beam is there but
the SEs are not being detected. If you have a Faraday
cup in or for your specimen holder, use a large aperture
(or none) and see if you get specimen current (I use
the stage alarm BNC connection to do this with a 10Meg Ohm
DVM. I=V/10^6).

Just a thought.

gary g.


At 05:53 AM 5/18/2001, you wrote:

} Listers -
}
} Those of you with LaB6 experience - is it possible for one to fail in such a
} way that it still shows a "normal" emission current on all the gauges, yet
} is not producing a findable beam?
} Last Monday we stripped our ESEM down for a bi-annual cleaning, inspected
} everything, including the LaB6 (which looked fine, and had been working well
} up to that point), put the whole thing back together, and were unable to
} find a beam. Now we've done this any number of times in the last 8 years,
} and it's never more than a few minutes to find the beam afterwards. Turn the
} condensor down low, select a 3mm "hole" instead of a projection aperture,
} and bingo, there it is - a big, shiny, beam like a floodlight.
} This time, nothing. Not a glimmer of "light" on the monitor, even though the
} LaB6 is "on", with 15KeV, and a "normal" emission current. We've had the
} column back apart 4 times now, to see if there's some physical blockage
} somewhere which might prevent the beam from reaching the chamber, but
} everything looks great. Even checked the performance of the chamber
} isolation valve, in case it was failing to open, but it's working properly.
} So I'm left with thinking maybe it's the LaB6, but in the past when we had
} one fail, it either suddenly began producing a strange, assymetrical beam,
} with normal emission current readings, or no beam at all, with no emission
} current showing. What gives? We have a new LaB6 on order, as a spare, so
} when it arrives I'll put it in, unless somebody has any other ideas....
}
} Frank Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada



From daemon Fri May 18 17:44:52 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Fri, 18 May 2001 18:44:00 -0400
Subject: politics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In response to Ron Vane's response to the dust analysis question: I think
you, Ron, should keep your political views about animal testing out of this
forum, especially since they seem to be oversimplified.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
jlagoy-at-bodycote-imt.com



From daemon Fri May 18 18:16:40 2001



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Fri, 18 May 2001 18:32:00 -0500
Subject: RE: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


the stachybotris link
http://gcrc.cwru.edu/stachy/

At 12:35 AM 5/18/01 -0500, Allen R. Sampson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri May 18 18:24:34 2001



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Fri, 18 May 2001 18:40:37 -0500
Subject: RE: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





this is the airborne fungi database
http://www.bio.psu.edu/People/Faculty/Whittam/apdbase/fungus.html

At 12:35 AM 5/18/01 -0500, Allen R. Sampson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri May 18 18:28:30 2001



From: colonel-at-natca.net ()
Date: Fri, 18 May 2001 18:26:29 -0500
Subject: Ask-A-Microscopist:Looking for a manual/info on a LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(colonel-at-natca.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, May
18, 2001 at 17:55:26
---------------------------------------------------------------------------

Email: colonel-at-natca.net
Name: M. B. Ingersoll

Organization: Schufeldt Academy

Education: 6-8th Grade Middle School

Location: Euless, TX, USA

Question: We are a homeschooling family. I have been all over the
Internet trying to find information (and hopefully a manual) for a
recently purchased, refurbished microscope.

The unit is a monocular, three objective (10x, 40x, 100x) self
illuminated model with mechanical staging and a variable condensor.
I am an Air Traffic Controller and not a microscopist so please
forgive me if I misuse any terminology.

From the markings I believe the manufacturer was SPI or PSI (the
letters S-P-I are arranged in that order in a diamond shaped logo).
There are two more numbers under the logo; 1804 (a model number?) and
23490 (preceded by "No." - presumably a serial number?). I was told
the unit is aproximately 30 years old.

Lastly, there is a small paper label on the underside of the base
with the word "JAPAN" on it.

Can you provide any information or point me to another source?

Thank you!

---------------------------------------------------------------------------


From daemon Sat May 19 11:27:20 2001



From: Jim Mabon :      mabon-at-uiuc.edu
Date: Sat, 19 May 2001 11:14:44 -0500
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ultimately this is a matter of personal preference and other requirements.
Are you an experienced Linux or Unix system administrator? Will you be
responsible for administration and security? What would your local network
administrator prefer/suggest? What languages and development environments for
you applications do you want to use? What database system will you use? These
are just some examples of the kinds of questions you should address first.

We have a Windows 2000 Server, with IIS 5.0 and FrontPage Server Extensions
and MS Access for databases. We chose this system for several reasons:
myself and the other developer are most comfortable developing software using
Visual Basic and vbscript; it is much easier to build and administrate than a
Linux system (at least for me), although I believe in any case the time
investment would be lower; at an educational institution cost of the licenses
is not much of an issue; you can automatically receive update notifications
(security); we just recently added a RAID 1 (mirroring) system to protect
from data loss and down time due to hard drive failures (trivial to set up);
preferred OS of our local network administrator; among others.

Of course, you might answer these questions entirely differently and your
answer might be Linux with Apache, java server pages(JSP) or PHP, MySQL, etc.



Chao-Ying Ni wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear colleagues,
}
} I'm going to build an EM lab server to run web and other applications,
} to share files, especially to have an interactive wed scheduling software
} to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I have
} narrow down the platform to Linux and Windows 2000. Could you please give
} me some inputs concerning the pros and cons of these two operating
} systems? Any comments and suggestions on the hardware selection are also
} more than welcome. Thanks in advance!
}
} Chaoying Ni
} Electron Microscopy Lab
} Dept. of Materials sci. and Eng.
} University of Delaware
} Newark, DE 19716
} cni-at-udel.edu



From daemon Sat May 19 14:54:37 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Sat, 19 May 2001 14:41:40 -0500
Subject: Animal Research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Respectfully, views on animal research seem to be perfectly suited to
civil discourse on this venue. Probably a significant number of the
listserver are involved with animal research. Issues affecting our
colleagues are definitely worth discussion and not to be censored by
labeling those opinions as political and oversimplified.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX 79008



____________________________

In response to Ron Vane's response to the dust analysis question: I think
you, Ron, should keep your political views about animal testing out of this
forum, especially since they seem to be oversimplified.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
jlagoy-at-bodycote-imt.com






From daemon Sat May 19 21:16:36 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 19 May 2001 21:09:44 -0500
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Go with the one you have the in house expertise to support. If you have to
support it yourself and don't want to spend time learning Linux or Unix
Windows is the easiest way. If you want to add a skill to your resume
Linux or Unix is a very marketable skill and makes a much more stable and
secure platform.

My Linux server has been up 252 days and it has two and a half years since
any software updates have been done except to patch some security flaws.
The only problems I have had were a break in and a processor that went
bad. I have had zero problems from software instability. But I had a good
system administrator set it up.

The secret to a stable system is once it is running like you want it leave
it alone. If you want to tinker with something get another system on line
to play with don't do it to the one that is in production. Once it is
working on the other system put it on the production system.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} } Dear colleagues,
} }
} } I'm going to build an EM lab server to run web and other applications,
} } to share files, especially to have an interactive wed scheduling
software
} } to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I
have
} } narrow down the platform to Linux and Windows 2000. Could you please
give
} } me some inputs concerning the pros and cons of these two operating
} } systems? Any comments and suggestions on the hardware selection are
also
} } more than welcome. Thanks in advance!
} }
} } Chaoying Ni
} } Electron Microscopy Lab
} } Dept. of Materials sci. and Eng.
} } University of Delaware
} } Newark, DE 19716
} } cni-at-udel.edu
}
}




From daemon Sat May 19 22:32:01 2001



From: COURYHOUSE-at-aol.com
Date: Fri, 18 May 2001 18:26:29 -0500
Subject: Ask-A-Microscopist:Looking for a manual/info on a LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--part1_93.b053bd0.2838934f_boundary
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SPI (and I forget what this stands for) is the company that you want.
They may still be in business and you might check through the search
engines for them one thing that I remember about SPI is that they also
imported micrometers and other materials also related to the precision
machine industry.

We regret that we have none of this company's catalogs or info here in the
archive,
our collection of catalogs and inst. books is growing though, If something
comes in
we will be happy to let you know. However if you do obtain any documentation
on this and wish to forward us a Xerox please feel free to send to:

coury house / smecc
5902 w palmaire ave
glendale az 85301

Good luck in the hunt!

Ed Sharpe Archivist for SMECC

} Subj: Ask-A-Microscopist:Looking for a manual/info on a LM
} Date: 5/18/01 7:33:30 PM US Mountain Standard Time
} From: colonel-at-natca.net
} To: Microscopy-at-sparc5.microscopy.com
}
}
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form. It was submitted by
} (colonel-at-natca.net) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, May
} 18, 2001 at 17:55:26
} ---------------------------------------------------------------------------
}
} Email: colonel-at-natca.net
} Name: M. B. Ingersoll
}
} Organization: Schufeldt Academy
}
} Education: 6-8th Grade Middle School
}
} Location: Euless, TX, USA
}
} Question: We are a homeschooling family. I have been all over the
} Internet trying to find information (and hopefully a manual) for a
} recently purchased, refurbished microscope.
}
} The unit is a monocular, three objective (10x, 40x, 100x) self
} illuminated model with mechanical staging and a variable condensor.
} I am an Air Traffic Controller and not a microscopist so please
} forgive me if I misuse any terminology.
}
} From the markings I believe the manufacturer was SPI or PSI (the
} letters S-P-I are arranged in that order in a diamond shaped logo).
} There are two more numbers under the logo; 1804 (a model number?) and
} 23490 (preceded by "No." - presumably a serial number?). I was told
} the unit is aproximately 30 years old.
}
} Lastly, there is a small paper label on the underside of the base
} with the word "JAPAN" on it.
}
} Can you provide any information or point me to another source?
}
} Thank you!
}
} ---------------------------------------------------------------------------
}
}




--part1_93.b053bd0.2838934f_alt_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} SPI (and I forget what this stands for) is the company that you want.
{BR} They  may still be in business and  you might check  through the search
{BR} engines for them one thing that I remember about SPI is that they also
{BR} imported micrometers and other  materials also related to the precision
{BR} machine industry.
{BR}
{BR} We regret that we have none of this company's catalogs or info here in the
{BR} archive,
{BR} our collection of catalogs and inst. books is growing though, If something
{BR} comes in
{BR} we will be happy to let you know. However if you do obtain any documentation
{BR} on this  and wish to forward us a Xerox please feel free to send to:
{BR}
{BR} coury house / smecc
{BR} 5902 w palmaire ave
{BR} glendale az 85301
{BR}
{BR} Good luck in the hunt!
{BR}
{BR} Ed Sharpe Archivist for SMECC
{BR}
{BR} {BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"} Subj: {B} Ask-A-Microscopist:Looking for a manual/info on a LM {/B}
{BR} Date: 5/18/01 7:33:30 PM US Mountain Standard Time
{BR} {I} From:    colonel-at-natca.net
{BR} To:    Microscopy-at-sparc5.microscopy.com
{BR} {/I}
{BR}
{BR}
{BR}
{BR} ------------------------------------------------------------------------
{BR} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} -----------------------------------------------------------------------.
{BR}
{BR}
{BR} Below is the result of your feedback form.  It was submitted by
{BR} (colonel-at-natca.net) from
{BR} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, May
{BR} 18, 2001 at 17:55:26
{BR} ---------------------------------------------------------------------------
{BR}
{BR} Email: colonel-at-natca.net
{BR} Name: M. B. Ingersoll
{BR}
{BR} Organization: Schufeldt Academy
{BR}
{BR} Education: 6-8th Grade Middle School
{BR}
{BR} Location: Euless, TX, USA
{BR}
{BR} Question: We are a homeschooling family.  I have been all over the
{BR} Internet trying to find information (and hopefully a manual) for a
{BR} recently purchased, refurbished microscope.
{BR}
{BR} The unit is a monocular, three objective (10x, 40x, 100x) self
{BR} illuminated model with mechanical staging and a variable condensor.
{BR} I am an Air Traffic Controller and not a microscopist so please
{BR} forgive me if I misuse any terminology.
{BR}
{BR} From the markings I believe the manufacturer was SPI or PSI (the
{BR} letters S-P-I are arranged in that order in a diamond shaped logo).
{BR} There are two more numbers under the logo; 1804 (a model number?) and
{BR} 23490 (preceded by "No." - presumably a serial number?).  I was told
{BR} the unit is aproximately 30 years old.
{BR}
{BR} Lastly, there is a small paper label on the underside of the base
{BR} with the word "JAPAN" on it.
{BR}
{BR} Can you provide any information or point me to another source?
{BR}
{BR} Thank you!
{BR}
{BR} ---------------------------------------------------------------------------
{BR}
{BR} {/BLOCKQUOTE}
{BR}
{BR}
{BR} {/FONT} {/HTML}

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Below is the result of your feedback form. It was submitted by
(colonel-at-natca.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, May
18, 2001 at 17:55:26
---------------------------------------------------------------------------

Email: colonel-at-natca.net
Name: M. B. Ingersoll

Organization: Schufeldt Academy

Education: 6-8th Grade Middle School

Location: Euless, TX, USA

Question: We are a homeschooling family. I have been all over the
Internet trying to find information (and hopefully a manual) for a
recently purchased, refurbished microscope.

The unit is a monocular, three objective (10x, 40x, 100x) self
illuminated model with mechanical staging and a variable condensor.
I am an Air Traffic Controller and not a microscopist so please
forgive me if I misuse any terminology.

From the markings I believe the manufacturer was SPI or PSI (the
letters S-P-I are arranged in that order in a diamond shaped logo).
There are two more numbers under the logo; 1804 (a model number?) and
23490 (preceded by "No." - presumably a serial number?). I was told
the unit is aproximately 30 years old.

Lastly, there is a small paper label on the underside of the base
with the word "JAPAN" on it.

Can you provide any information or point me to another source?

Thank you!

---------------------------------------------------------------------------


--part1_93.b053bd0.2838934f_boundary--


From daemon Sun May 20 04:15:59 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sun, 20 May 2001 04:10:21 -0500
Subject: RE: MIcrospheres, another use for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may have answered your own question. Opal makes a good SEM sample when
fractured, acid etched and coated. A nicely packed lattice of very round
spheres, I use one as simple test sample, along with a semiconductor die
and NIST magnification and resolution standards. A small chunk can
virtually last forever.

On Friday, May 18, 2001 7:57 AM, Robert H. Olley
[SMTP:r.h.olley-at-reading.ac.uk] wrote:
}
}
} Hello all Listers,
}
} Some time ago, someone in our chemistry department made a aqueous
} suspension of silica microspheres, about half a micron in diameter. If
} this was allowed to dry out, the spheres formed a close-packed lattice,
} similar to that in opal which gives rise to the lovely diffraction
} colours. When examining this lattice under the SEM, we found that this
} made an ideal target for the AUTO aSTIGmatism control, so when examining
} something bland such as a fine textured plastic surface, one could use a
} clump of these spheres as a target and then have the optics just right
} for examining the bland specimen.
}
} Alas, the suspension of spheres has degenerated into some sort of "goo",
} and no more is available. Are there any other sources of spheres of
} this size? The 1 to 8 micron space spheres are probably a little too
} big, and I am a bit wary of what magnetite spheres might do in the
} electric field of the SEM.
}
} +----------------------------------------------------------------+
} Robert H.Olley
} J.J.Thomson Physical Laboratory
} University of Reading
} Whiteknights
} Reading RG6 6AF
} England
} +----------------------------------------------------------------+
} Phone:
} {direct line +44 (0) 118 9318572
} {University internal extension 7867
} Fax: +44 (0) 118 9750203
} Email: R.H.Olley-at-reading.ac.uk
} URL: http://www.reading.ac.uk/~spsolley
} +----------------------------------------------------------------+
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sun May 20 16:02:48 2001



From: Larrrry
Date: Mon, 21 May 2001 13:33:11 -0700
Subject: ForUaGoooooodCellAccessDeaL 17492

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



*******************
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From daemon Sun May 20 17:57:11 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Mon, 21 May 2001 08:52:57 +1000
Subject: Re: LaB6 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank,
I thing you are very lucky to normally find the beam so quickly. We have
an E3 ESEM and on that beast it can take ages to find the beam after
routine service.
Regards
JVN

Frank Thomas wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Listers -
}
} Those of you with LaB6 experience - is it possible for one to fail in such a
} way that it still shows a "normal" emission current on all the gauges, yet
} is not producing a findable beam?
} Last Monday we stripped our ESEM down for a bi-annual cleaning, inspected
} everything, including the LaB6 (which looked fine, and had been working well
} up to that point), put the whole thing back together, and were unable to
} find a beam. Now we've done this any number of times in the last 8 years,
} and it's never more than a few minutes to find the beam afterwards. Turn the
} condensor down low, select a 3mm "hole" instead of a projection aperture,
} and bingo, there it is - a big, shiny, beam like a floodlight.
} This time, nothing. Not a glimmer of "light" on the monitor, even though the
} LaB6 is "on", with 15KeV, and a "normal" emission current. We've had the
} column back apart 4 times now, to see if there's some physical blockage
} somewhere which might prevent the beam from reaching the chamber, but
} everything looks great. Even checked the performance of the chamber
} isolation valve, in case it was failing to open, but it's working properly.
} So I'm left with thinking maybe it's the LaB6, but in the past when we had
} one fail, it either suddenly began producing a strange, assymetrical beam,
} with normal emission current readings, or no beam at all, with no emission
} current showing. What gives? We have a new LaB6 on order, as a spare, so
} when it arrives I'll put it in, unless somebody has any other ideas....
}
} Frank Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Sun May 20 19:50:42 2001



From: hines-ta-at-actx.edu ()
Date: Sun, 20 May 2001 19:46:55 -0500
Subject: Ask-A-Microscopist: Info. on Nomarski

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(hines-ta-at-actx.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, May
20, 2001 at 16:03:16
---------------------------------------------------------------------------

Email: hines-ta-at-actx.edu
Name: Tracey Hines

Organization: Amarillo College

Education: Undergraduate College

Location: Amarillo, Texas

Question: I am having a difficult time finding any biographical information
on George Nomarski. Do you know of any web sites or books that will
be useful in finding information like birthdate, etc.?

---------------------------------------------------------------------------


From daemon Mon May 21 05:27:39 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Mon, 21 May 2001 12:19:10 +0200
Subject: Re: Microspheres (fwd)

Contents Retrieved from Microscopy Listserver Archives
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Nick

Someone gave me a recept to make a sample with Tin balls. You need a
polished aluminium sample, a old vaccum coater with a unregulated low
voltage/ high current supply (one with a big variac and a 4V-12V/1000A
transformer). You put some tin (Sn) in a Mo or W boat, (and of course your
sample in the oposite) and you heat it under vacuum very quickly. It makes
an "explosion" coating, and you will find tin balls, with a great
dispersion in size on your aluminium sample (a few 100 nm to a few
microns). It is a good test for backskatered detector and for SE
resolution. With some practice, you schould be able to control the size,
size distribution and density of tin balls.

I didn't try this receipt (not enouhgt time to try all what I want ! ),
but according to my experience in thin film technology, it seems to be
credible.

I saw also something like that to sell in a microscopy supplies catalogue,
but I don't remember where, I think it was someone in Germany.

By


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


On Thu, 17 May 2001, Nicol Aitken wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} }
} } Hello Listers,
} } I was wondering if anyone out there can help me out. I am looking
} } for a solution of uniform particles (spheres would be nice) of a heavy
} } material so that it will show up nicely in backscatter. I also require
} that
} } the particle size be between 2 and 5 micron. If anyone knows of something
} } like this and where I can order it from, It would help me out.
} } Thanks in advance
} } Nick
}
}




From daemon Mon May 21 08:25:19 2001



From: meehall-at-mac.com ()
Date: Mon, 21 May 2001 08:20:20 -0500
Subject: Ask-A-Microscopist: Which Microscope for photographs of diatom

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(meehall-at-mac.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, May
20, 2001 at 23:49:07
---------------------------------------------------------------------------

Email: meehall-at-mac.com
Name: michael n maloney

Organization: san francisco police dept

Education: Graduate College

Location: san francisco

Question: i'm looking for the most appropriate microscope for fine
art photographs of diatom and radiolarian sized objects. i understand
that a true darkfield illumination source is preferable. a support
for a heavy camera is also required. I use 35 mm (Nikon F5) and a 4x5
camera. please give me some thoughts on this

---------------------------------------------------------------------------


From daemon Mon May 21 08:28:35 2001



From: Nicholas W. M. Ritchie :      nritchie-at-aspexllc.com
Date: Mon, 21 May 2001 08:26:11 -0500
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
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I use both Windows 2000 and Linux and like them both but they are as
different as
night-and-day. When all is said and done, both can be reliable,
workhorse servers.

Here are a couple of issues you might consider:
Administration - Windows 2000 Server is not easy to manage but it
is substancially
easier than Linux. Most Linux servers are set up by long-time Unix
gurus. To them the
obscure commands and syntax are second nature and they understand the
sublties of
network administration. If this doesn't describe you then it is
probable that the lack
of handholding in the Linux world will make configuring your server
an adventure. Some
of the standard Linux installs (Red Hat for example) make setting up
a server easier
but at the expense of leave gapping security holes.
Clients - Are your client computers running Unix, Windows or ?
While Linux can
interoperate with Windows, it is easier to configure a Linux system
to work with other
Linux/Unix systems. Similarly, Win2k is easy to configure to work
with Windows and a
little harder to configure to work with Unix/Linux.

In summary, either one can work. Linux requires more
knowledge and experience
but the price is right.

Hope this helps.

Nicholas


5/19/2001 10:09:44 PM, "Gordon Couger" {gcouger-at-couger.com} wrote:

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From daemon Mon May 21 10:26:21 2001



From: Susanne Stemmer :      stemmer-at-rice.edu
Date: Mon, 21 May 2001 10:18:56 -0500
Subject: TEM postdoc position

Contents Retrieved from Microscopy Listserver Archives
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*************************************************************
Postdoctoral Position in Transmission Electron Microscopy - Rice University

Rice University, Department of Mechanical Engineering and Materials Science,
located in Houston, TX, is seeking candidates for a postdoctoral position in
transmission electron microscopy of multicomponent oxide thin films.
Applicants should have extensive and demonstrated experience in several areas
of TEM and a strong background and interest in materials problem solving.
Preference will be given to candidates with experience in several TEM
techniques.
Facilities at Rice include a JEOL 2010, field-emission SEM and high-resolution
X-ray diffractometers, as well as state-of-the-art sample preparation. The
project will be carried out in close collaboration with the University of
Houston using a state-of-the-art field-emission TEM (JEOL 2010F), with annular
dark-field detector, Oxford link EDS, Gatan GIF and STEM capabilities.
The position is available
immediately. Duration about 1-2 years, salary is commensurate with
qualifications. Candidates with a Ph.D. in Materials Science or Physics will
be given preferred consideration.
Interested candidates should send a curriculum vitae, publication list and the
names of at least three references with their contact addresses to:

Prof. Susanne Stemmer
Rice University
Department of Mechanical Engineering and Materials Science
MS 321
6100 Main Street
Houston, TX 77005-1892
stemmer-at-rice.edu
*************************************************************


From daemon Mon May 21 10:56:29 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Saturday, May 19, 2001 2:09 PM
Subject: Animal Research

Contents Retrieved from Microscopy Listserver Archives
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We microscopists have focused tools with narrow fields of view. Toxicity is
a very broad problem which our tool can see only a small portion.

My reply was not meant to reflect on my views of animal research but was an
example of the problem of satisfying two very different portions of the
technophobic community around us. As such it was very oversimplified.

I agree that animal research is not a good topic for this forum.

Ronald Vane
XEI Scientific

-----Original Message-----
} From: Chuck Butterick {cbutte-at-ameripol.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com} ;
JLaGoy-at-bodycote-imt.com {JLaGoy-at-bodycote-imt.com}




From daemon Mon May 21 11:27:31 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 21 May 2001 09:22:03 -0700 (PDT)
Subject: Re: Dust analysis?

Contents Retrieved from Microscopy Listserver Archives
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Jon:
Let's try to get back to a reasonable answer here. Like you, I think that
getting into it yourself can be problematic. So, for labs that do the work:
McCrone and RJLee are the two I am most familiar with. I would guess there
are others, and have no interest in the two mentioned, nor do I have
anything against any lab. Labs like this are easily searchable on the
internet, and I think you could also find them under one of the microscopy
web resources (sorry-having a total medicare moment on names and such).
Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
On Thu, 17 May 2001 15:26:34 -0700, Jon Krupp wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hi:
|
| This is a weird question, it could only happen in Santa Cruz.
|
| One of our EH&S people stopped by today to ask if we could analyze some
| dust from one of the science buildings on campus. I said 'Maybe' and
| listened to the rest of the story.
|
| He told me that a new custodial employee was afraid that the dust in one
of
| the science buildings was full of toxic material and wanted it tested.
The
| building is a 30 year old structure that has had various sorts of biology
| and chemistry teaching and research labs. As far as I know there has
never
| been any sort of problem there and EH&S keeps track of things like
| radiation and highly toxic situations.
|
| I was reluctant to get involved. Our EDS could probably see some things
and
| I could recognize some mold spores and cotton fibers, but who am I? I
don't
| have any training or certification in toxic dust analysis. Plus I
probably
| already think this guy is a little paranoid about dust, so I probably
| wouldn't find anything even if it was there.
|
| Are there labs that can do this sort of analysis and can certify that
there
| is nothing toxic to worry about in a sample of dust? How does one sample
| for these things and how do we respond to this kind of request. What are
| the odds that the dust is anything more than just dust? What is dust
| anyway?
|
| I promised our EH&S guy that I would ask around among the smartest group
of
| folks I know. So what do you think?
|
|
| Jonathan Krupp
| Microscopy & Imaging Lab
| University of California
| Santa Cruz, CA 95064
| (831) 459-2477
| jmkrupp-at-cats.ucsc.edu
|
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon May 21 11:36:52 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 21 May 2001 09:32:22 -0700 (PDT)
Subject: Re: TEM Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Prof Barajon:
Just a minor addition to the other comments (I have used variations of all
of them). While not familiar with the referenced paper, I would add that
you can (perhaps should) reduce your fixation times. I use 45 to 60 min in
the aldehyde and 30 to 45 min in osmium. I can't remember the references,
but there is an older reference to using methylene blue for en bloc staining
of the epoxy-embedded cells. This really enhances the ability to locate
areas of interest after embedding. (I believe the paper was in Stain
Technology, probably in the 70's). This must be a senior moment day--I
can't remember details on anything.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
On Thu, 17 May 2001 10:44:03 +0200, Prof. Barajon wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Subject: TEM- cell colture fixation and embedding
|
| I need to study the ultrastructural aspects of a cell line grown on petri
| dishes. Any suggestion for the best fixation and embedding protocol (no
| pellets, just fixation and embedding in the dish itself) ?
|
| thanks
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon May 21 17:30:59 2001



From: Rhonda Stroud :      stroud-at-nrl.navy.mil
Date: Mon, 21 May 2001 18:07:40 -0400
Subject: TEM: Be film grids?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all.

I'm looking for a suggestion for appropriate film support grids for making
EELS and EDS measurements of 1 to 10 nm particles. I would use plain
carbon, except that the particles will be a mixture of carbide, oxide,
metal and silicate phases. I need to measure the C, O, N, Si, Mg and Fe
content of the particles, so C, SiO2 or Si3N4 support films all would make my
life difficult.

Has anyone made Be film grids? Any other suggestions?

Thank in advance.

Rhonda Stroud

Research Physicist
Naval Research Laboratory
4555 Overlook. Ave SW
Washington, DC 20375


From daemon Mon May 21 20:46:32 2001



From: Chao-Ying Ni :      cni-at-udel.edu
Date: Mon, 21 May 2001 21:39:54 -0400 (EDT)
Subject: Double tilt holder wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings!

I'm looking for a second-hand 2000FX double tile holder, or the compatible
2-tile JEOL100 holder. Anybody having the information on this please let
know. Thanks much!

Chaoying Ni
EM Lab
Materials Sci and Eng
University of Delaware




From daemon Mon May 21 21:32:30 2001



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 21 May 2001 19:25:06 -0700
Subject: Amray FE filament replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Has anyone sucessfully done an Amray FE filament replacement for the newer
"305" guns?

I think there is more to it than meets the eye.

Thank You,

Earl



From daemon Mon May 21 22:35:54 2001



From: Nick Bulloss :      bulloss-at-geo.utep.edu
Date: Tue, 22 May 2001 07:56:01 -0600
Subject: SEM -service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would agree that familiarity with the operating environment is important
for timely performance of server administration tasks-documentation and
advocacy groups exist for both platforms, however. It might be worth while
to become familiar with a UNIX like operating system (such as Linux) if one
has the luxury of time (I'll explain momentarily). Cost may be an
issue-Linux is free. Both OSes run on the x86 architecture.
It seems that your choice(s) of application software should be a
heavywieght factor in your decision. At the risk of over-simplification
I'll just say that most proprietary "out of the box" solutions are developed
for Windows/NT. There is a huge installed base of Wintel machines, and
there are powerful software development tools for this platform which allow
software developers to develop large complicated programs very rapidly.
Linux has the advantage that many programs written to run under the
various flavors of UNIX will compile and run. Many cutting edge scientific
applications, including image processing utilities, are developed by
researchers who are experienced coders and prefer to work under the UNIX
platform. The reason for this is partially historical-in the past only large
UNIX mainframe servers had the horsepower for image manipulation. More
often than not these software tools are available free of charge to fellow
researchers and are provided as open-source (one can view and modify the
original code to suit specialized situations).
So it really boils down to an ease of use vs. scalability/extensibility
issue with cost, and perhaps minor hardware decisions (sometimes peripherals
are difficult to configure under Linux ie. video cards, scanners etc...).
In the long run, however, time spent now learning to work with Linux may pay
off: the heart of the OS (the kernel) evolves continually in response to
the needs of users, and the kernel can be upgraded without disrupting other
parameters of the OS. Also, Linux runs on most (if not by now all)commonly
encountered chip architectures so one may have several different kinds of
machine (PC, Mac, SUN) all running the same operating system.

Karl Garsha
www.uwm.edu/~keg
----- Original Message -----
} From: "Chao-Ying Ni" {cni-at-udel.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, May 18, 2001 9:36 AM


Hi folks,
We have an ancient ISI (Akashi) Mini-SEM which is need of a
service/overhaul. Could anyone recommend service companies that would be
able to deal with such an old instrument? Companies located in the
southwest would be preferable.
Thank you for your time,
Nick Bulloss



******************************************
Nick Bulloss
Analytical Facilities Technician
Department of Geological Sciences
University of Texas at El Paso
500 West University Ave
El Paso, TX 79968-0555
Office: (915) 747-5440
Lab: (915) 747-5184
Fax: (915) 747-5073

******************************************



From daemon Tue May 22 09:12:27 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 22 May 2001 10:05:53 -0400
Subject: Re: Dust Analysis

Contents Retrieved from Microscopy Listserver Archives
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Jon,
Your question raises questions whose import we should all
understand. Getting involved, as Dr. Moretz said is 'problematic', but my
response is much stronger. Getting involved is potentially hazardous to
your health!
You have answered your big question. "Who am I?" And you mean, not
merely how much of what is required can "I" accomplish? You are correct to
hesitate. The implications of involvement in such an institutional question
are NOT trivial.
First of all, as an institutional employee, you will have NO legal
credibility except where you assert a positive finding of institutional
fault, and the institution, would then be obligated to find an external
source to either defend or deny your assertion. The only way by which
this morass can be avoided is if an institutional policy directs you to
perform such internal testing in order to determine 'an indication'.
Second, there is NO good ground for you in such a question, and the
person who requested some help was ill-advised and, worse, ill-prepared, for
this personnel problem. The question raised by the 'concerned' employee was
a "black hole". His/her supervisor should have immediately sent her/him to
the appropriate Human Resource representative. The employee's concern was
instant grounds for transfer, and immediate grounds for the institution to
guard itself from her/his problem. Human resources should have a record of
personnel performance in the workplace in question. If there has been an
attendance problem due to illness among individuals working in the building
or in spaces within the building, Human Resources should probably initiate
an investigation or an upward 'chain of concern' if there is no clear policy
that they can follow.
You have raised the question of our own awareness and understanding
of the difference between good science and good evidence as viewed by
different interests within our society.
We, who have been trained through graduate school for the jobs we
have performed PRESUMABLY understand the inherent hazards of our jobs. Oil
vacuum systems in cool but poorly ventilated rooms. Photographic darkrooms
with inadequate ventilation. Formaldehyde, formalin, paraformaldehyde,
paraldehyde, all peroxides and peroxide formers, X-Rays, 32P, Xylene,
Propylene Oxide, MeOH, dissolving osmium tetroxide crystals, Toluene, human
tissues and fluids, Latex, Hepatitis B, etc. Most of us as microscopist
have taken 'the list' with the job, and very few of us have been crippled by
the hazards. Yet; hazards there are. Those otherwise capable individuals
who have been selected out of our zone in the sciences have probably gone
early rather than late. There are rumors about contact dermatitis from
photographic chemicals, but few of us know anyone who has suffered that
outcome.
The dangers of our work become suppressed in us. I remember hearing
a muffled 'whump' when I was a graduate student, then looking out of a
nearby window and seeing a refrigerator door lying on the grass three
stories down from a blown-out window in the chemistry building. Later that
day, we were asked to search all refrigerators for explosives, because a
'fridge' containing a can of ethyl ether had exploded (no injuries). Six
cans from our floor subsequently appeared in the lone chemical fume hood,
and I have never forgotten my silent concern when I removed mine. "How will
I keep it from evaporating now?" Thirty-five years ago, the label on a can
of ethyl ether warned against storing it in a refrigerator that was NOT
explosion proof. We worked in a five story building, and there was not one
such refrigerator among the thirty or so that I could count. I need not ask
how many are to be found today. More, but not enough - "they are VERY
expensive!" Is there one in every lab that uses ethanol at less than 4
degrees?
There ought to be a continuing thread on this server about hazard
and safety concerns. My self-doubt raised concerns identical to yours
twenty years ago, and only a well-developed sense of self-preservation
caused me to respond to an 'institutional' request for help with a very long
list of reasons why I was obligated to refuse. In the process I lost favor.
My first, but not only, run in with administration about job description and
available resources.
If my colleagues wonder why I have been so long-winded, it may be
that you could use some long-winded support in your refusal to become
involved in an issue for which you apparently were not hired and for which
you have not been provided adequate authority to address.

Good luck Jon, your concern is not 'weird', and IT has happened a
lot!


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Tue May 22 09:40:32 2001



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Tue, 22 May 2001 14:36:11 -0000
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all!
I have a couple of steel wires which I want to watch in TEM. The problem is,
that these wires are very fine...less than 100 microns. I want to know if
anyone has any ideas about how to prepare the samples for TEM.
Regards
Praveena
Center for advanced materials
University of Massachusetts, Lowell
Lowell, MA
PH:978-934-3411
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Tue May 22 10:28:59 2001



From: Kim Riddle :      riddle-at-bio.fsu.edu
Date: Tue, 22 May 2001 11:26:51 -0400
Subject: sectioning soft polymers

Contents Retrieved from Microscopy Listserver Archives
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To all,

We are having a problem with rippling when cutting 60 micron hydrogel
sections. Does anyone know a way to eliminate this rippling?

Thanks, Kim

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Kimberly A. Riddle
Florida State University tel: 850.644.6519
Biological Science Imaging Resource
119 Bio Unit I, 4370 fax: 850.644.0481
Tallahassee, FL 32306 riddle-at-bio.fsu.edu
http://bio.fsu.edu/~taylor/imaging
~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue May 22 10:51:23 2001



From: Connolly, Brett M :      brett_connolly-at-merck.com
Date: Tue, 22 May 2001 11:46:57 -0400
Subject: LCM Arcturus vrs. Leica

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Hi,

Help please, I'm in the market for a laser capture microdissector and would
like your comments, recommendations, experiences, pro & cons, etc. of the
Arcturus Pixcell II vrs. Leica's apparatus. We have seen them both, but need
unbiased input from current users.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
ph. 215-652-2501
fax. 215-652-2075
email. brett_connolly-at-merck.com



From daemon Tue May 22 11:06:43 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Tue, 22 May 2001 12:02:14 -0400
Subject: Re: Amray FE filament replacement

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Earl,

Ladd Research has been doing tungsten filaments for many years, but we
do not do the AMRAY FE filaments. We agree that it "is more than meets
the eye" with these and in this case it it is probably better to go
direct to the instrument manufacturer.

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



Earl Weltmer wrote:
}

} Dear Listers,
}
} Has anyone sucessfully done an Amray FE filament replacement for the newer
} "305" guns?
}
} I think there is more to it than meets the eye.
}
} Thank You,
}
} Earl


From daemon Tue May 22 11:25:07 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 22 May 2001 09:20:44 -0700 (PDT)
Subject: Re: TEM: Be film grids?

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Rhonda:
Boy, does this bring back some really ancient memories (clear back to the
late 60's!). Be films can be made the same way as C, and we evaporated Be
onto glass slides in a standard evaporator (back then it was an old
Kinney--but any regular evaporator will work). The *major* problem is Be
toxicity, and in the vapor or dust form, Be is extremely toxic. We set up a
"bell jar -in a- bell jar" system. We used a large heavy-glass beaker
(probably a 2L Kimax heavy wall beaker) over the electrodes to reduce
contamination of the entire unit. It is also wise to set up at least one
trap in the forepump line as well as use LN2 on the diff pump. There may be
some old literature out there on more specifics, but I don't know where.
You may also find that some of the EM suppliers (e.g. SPI, EM Sciences,
Pella, Fullam, Ladd, Tousimis, Polysciences)(sorry if I missed anyone) can
supply you with the appropriate substrates. That's surely easier, altho'
maybe not cheaper.

Roger

On Mon, 21 May 2001 18:07:40 -0400, Rhonda Stroud wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hello all.
|
| I'm looking for a suggestion for appropriate film support grids for
making
| EELS and EDS measurements of 1 to 10 nm particles. I would use plain
| carbon, except that the particles will be a mixture of carbide, oxide,
| metal and silicate phases. I need to measure the C, O, N, Si, Mg and Fe
| content of the particles, so C, SiO2 or Si3N4 support films all would
make my
| life difficult.
|
| Has anyone made Be film grids? Any other suggestions?
|
| Thank in advance.
|
| Rhonda Stroud
|
| Research Physicist
| Naval Research Laboratory
| 4555 Overlook. Ave SW
| Washington, DC 20375
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue May 22 11:38:10 2001



From: Richard R. Vanfleet :      vanfleet-at-physics.ucf.edu
Date: Tue, 22 May 2001 12:33:09 -0400
Subject: Re: TEM: Be film grids?

Contents Retrieved from Microscopy Listserver Archives
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I am approaching a similar need and have been looking into Boron support
films. It has been done in the past although I do not have the reference
handy. We hope to try making some in the next few months.

I too am interested in other options as I am interested in both Carbon,
Silicon, and Boron containing samples. The Boron films will only trade
conflicts and not remove the issue.

Richard Vanfleet


At 06:07 PM 5/21/01 -0400, Rhonda Stroud wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue May 22 11:38:11 2001



From: Rhonda Stroud :      stroud-at-nrl.navy.mil
Date: Tue, 22 May 2001 12:23:30 -0400
Subject: Be films

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who replied to my query on support grids.

The solution that seems best suited to my particular problem
is amorphous Ge film. I'm now deciding whether to deposit the film myself,
or whether to buy it.

For the curious Ge is the best because:

(1) No EDS or EELS interference with elements of interest
(2) It's possible to make thin amorphous Ge films, so the background
contrast will
not be problem for high imaging (as apposed to Be and Al)
(3) I can't use holey films because the samples will actually be collected
directly on the grids during a rocket flight. Simultaneous collection on C
and SiO is possible, but would
make it difficult to get all info on each particle.


Rhonda Stroud

Research Physicist
Naval Research Laboratory
Washington, DC 20375



From daemon Tue May 22 12:05:19 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Tue, 22 May 2001 13:08:07 -0400
Subject: Re: SPI microscope

Contents Retrieved from Microscopy Listserver Archives
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I'm wondering...I purchase microscope supplies from SPI Supplies in West
Chester, PA. Their phone no. is 800-242-4774. Perhaps they can help you.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
jlagoy-at-bodycote-imt.com



From daemon Tue May 22 13:17:31 2001



From: Robert Schoonhoven :      rschoonh-at-sph.unc.edu
Date: Tue, 22 May 2001 14:18:06 -0400
Subject: SEM low mag partical size analysis

Contents Retrieved from Microscopy Listserver Archives
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OK list, once again I come looking for some help. We are looking at
some dirt (OK, soil) samples and I am looking for the easiest way to
measure the grains(?) and the spaces between them..... Being cell and
not dirt orientated I could use some help from the material sciences
people. Is there a cheap or freeware program that will do this for me
or do I have to basterdize my bio-imaging software (BioQuant TCW) to do
this??

As usual I'm looking for the least labor intensive method.
--
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)


From daemon Tue May 22 14:00:11 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 22 May 2001 14:53:48 -0400
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
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You can use Field Ion Microscopy sample prep techniques to prepare very sharp needles of the wires. Basically, you can dip them in a beaker and electropolish them. The liquid/air interface will preferentially polish them. You need to move the interface up and down the sample by moving either the sample or liquid. If the wires are long enough, you can float electrolyte on top of CCl4 layer and you can get two samples when the bottom part drops off. Look up some of the standard electropolishing solutions and conditions. One is based on percholoric/butylcelsolve and the other on chromic acid/acetic acid -I think.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Praveena Bhaskara [mailto:bubbyp-at-hotmail.com]
Sent: Tuesday, May 22, 2001 10:36 AM
To: Microscopy-at-sparc5.microscopy.com


Hi all!
I have a couple of steel wires which I want to watch in TEM. The problem is,
that these wires are very fine...less than 100 microns. I want to know if
anyone has any ideas about how to prepare the samples for TEM.
Regards
Praveena
Center for advanced materials
University of Massachusetts, Lowell
Lowell, MA
PH:978-934-3411
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Tue May 22 15:01:42 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 22 May 2001 15:56:18 -0400
Subject: RE: Ask-A-Microscopist: Which Microscope for photographs of diat

Contents Retrieved from Microscopy Listserver Archives
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} You could call around to the small colleges in your area - as well as used
} microscope vendors - for a late 1960's through mid 80's upright, retired,
} transmitted fluorescence microscope from Leitz, Zeiss or B&L and Nikon.
} If you are lucky, you will find one that still has its substage dark field
} condenser in the nearby desk drawer.
}
} You could also consult geology and chemistry departments looking for a
} microscope that has Leitz or Zeiss Ultropak objectives. These objectives
} were/are used in epi-illuminated systems and were equipped with internal
} conical mirrors that created a 'dark' field illumination. In combination
} with transmitted light, such objectives were recommended for photography
} of autoradiograms.
}
} Hope this helps.
}
Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: meehall-at-mac.com
} Sent: Monday, May 21, 2001 9:20 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Which Microscope for photographs of
} diatom and radiolarians
}
}
}
} Email: meehall-at-mac.com
} Name: michael n maloney
}
} Organization: san francisco police dept
}
} Education: Graduate College
}
} Location: san francisco
}
} Question: i'm looking for the most appropriate microscope for fine
} art photographs of diatom and radiolarian sized objects. i understand
} that a true darkfield illumination source is preferable. a support
} for a heavy camera is also required. I use 35 mm (Nikon F5) and a 4x5
} camera. please give me some thoughts on this
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Tue May 22 15:02:17 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 22 May 2001 15:58:14 -0400
Subject: RE: Be film grids?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Vollenweider, H.J., Al-Be films, J. Microscopy, 16: 247(1973) from
} Principles and Techniques of E M, Hayat, 3d Ed., CRC Press, 1989.
}

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Rhonda Stroud
} Sent: Monday, May 21, 2001 6:07 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM: Be film grids?
}
}
} Hello all.
}
} I'm looking for a suggestion for appropriate film support grids for making
} EELS and EDS measurements of 1 to 10 nm particles. I would use plain
} carbon, except that the particles will be a mixture of carbide, oxide,
} metal and silicate phases. I need to measure the C, O, N, Si, Mg and Fe
} content of the particles, so C, SiO2 or Si3N4 support films all would make
} my
} life difficult.
}
} Has anyone made Be film grids? Any other suggestions?
}
} Thank in advance.
}
} Rhonda Stroud
}
} Research Physicist
} Naval Research Laboratory
} 4555 Overlook. Ave SW
} Washington, DC 20375
}
}


From daemon Tue May 22 15:05:44 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Tue, 22 May 2001 16:02:06 -0400
Subject: Generation of in situ osmium tetroxide

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Hi,

I do staining of TEM thin sections of microporous polymer systems, rubber compounds and polymer based automotive coatings.

I use RuO4 mostly for staining and would like to switch to OsO4 occasionally. I use a very safe procedure in which I generate very small amounts of RuO4 vapors in a petri dish in situ. This limits problems with amounts handled, safety and minimizes disposal.

In the literature I see articles and also web sites that talk about using hot fuming nitric acid (impractical for me to keep heated), hot nitric acid (ditto) and hydrogen peroxide on "Osmium Compounds" or metal. Suggestions are made at some sites that osmium dioxide is used. OsO2 does not seem to be sold at any chemical supply houses nor is it in any EM supplier catalogs I have.

I DO NOT want to use ampoules of OsO4 or RuO4 and have them stored around the lab in sealed vials. I had an unscored ampoule of RuO4 spontaneously break once and it was not pleasant for my refrig' or me.

Question(s):
Have you ever experimented with or know of any osmium compound(s) used to in situ generate osmium tetroxide vapors from one of these compounds?
Is the procedure safe and does it generate small amounts of waste?
What do you use as a staining indicator?
Butadiene rubber or a solution of it, works for me with OsO4.

My aim here is not to find a 'cheap' way to make OsO4. Os costs a lot of money no matter what you buy or where.

Thank you for any help you can give or ideas.

Paul Beauregard
PPG Industries
Senior Research Associate
Monroeville, PA 15146




From daemon Tue May 22 15:40:22 2001



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Tue, 22 May 2001 16:44:12 -0500
Subject: coolwell chillers

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Hi,
I'm having a problem with the Coolwell Chiller (SE - Style) for my TEM.
Everything is fine for a few hours then the temperature goes way up. The
guys working on it checked the refrigerant level and the gauge keep
fluctuating between 20 and 60. They wanted me to find out if that is
normal. Any help?
Thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Tue May 22 17:19:27 2001



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Tue, 22 May 2001 18:15:20 -0400
Subject: re: coolwell chillers

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Hi Beth,
I have 3 of these SE units and know nothing about the refrigerant but have
replace 2 temperature controllers because of intermittent climbing
temperature.
Is the red 'heat' indicator on when it overheats (make sure the bulb is
good first)? When the temperature goes up try adjusting the controller down
with a screwdriver. If cooling begins (blue indicator comes on) most likely
the switch needs replacing and the compressor is ok. I tried cleaning the
contacts but that didn't last. If the blue 'cool' light comes on but the
unit's still overheating you may have a refrigeration problem.

Coolwell is out of business so to give credit where it is due here is a
letter I received last year when I was looking for parts.
............................................
} We replaced the original thermostat on our Coolwell SE unit with an "ETC
} Single Stage Electronic Temperature Controller" and a "1309007-044 ETC
} Temperature Sensor" from Ranco (8115 U.S. 42 N., Plain City, Ohio, 43064).
} The unit bolts right to the front of the Coolwell chiller and works just
} fine. It works over a tempertature range of -30F to +220F and a
} differential of 1F to 30F. I seem to recall that the whole shebang was
} less than $100 and our campus refrigerator guy installed it with no
} problems. I can fax the spec sheets to anyone who is interested. I
} believe I got the idea from Maggy Piranian through this list.

} Bob
} Dr. Robert R. Wise
} Associate Professor of Plant Physiology
} Department of Biology
} University of Wisconsin Oshkosh
.............................................
Regards,
Jim

} X-Comment: UCONNVM.UConn.Edu: Mail was sent by sparc5.microscopy.com
} Mime-Version: 1.0
} Date: Tue, 22 May 2001 16:44:12 -0500
} To: microscopy-at-sparc5.microscopy.com
} From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
} Subject: coolwell chillers
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Tue May 22 19:48:40 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 22 May 2001 18:29:53 -0600
Subject: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
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Could you provide more information about what exactly you want to do? It
might help people to suggest preparation techniques. do you need cross
sections, look along the wires, perhaps the separation between wires?

You probably have to embed the samples in epoxy. Perhaps you can use a
microtome to slice them?

Or take a whole bunch of them, embed them and then ion mill?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


-----Original Message-----
} From: Praveena Bhaskara [mailto:bubbyp-at-hotmail.com]
Sent: Tuesday, May 22, 2001 8:36 AM
To: Microscopy-at-sparc5.microscopy.com


Hi all!
I have a couple of steel wires which I want to watch in TEM. The problem is,

that these wires are very fine...less than 100 microns. I want to know if
anyone has any ideas about how to prepare the samples for TEM.
Regards
Praveena
Center for advanced materials
University of Massachusetts, Lowell
Lowell, MA
PH:978-934-3411
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Tue May 22 20:03:44 2001



From: Gary Gill :      garygill-at-dcla.com
Date: Tue, 22 May 2001 20:02:01 -0500
Subject: Source of used 4X objective

Contents Retrieved from Microscopy Listserver Archives
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Can anyone point me to a source for such an objective for an old AO
Microstar Series 110 microscope? I tried eBay once, but the objective
didn't match the microscope requirements. Thanks.

Gary Gill


From daemon Tue May 22 20:03:44 2001



From: Gary Gill :      garygill-at-dcla.com
Date: Tue, 22 May 2001 20:01:48 -0500
Subject: Troubleshoot visual blurring during microscopy of acellular field

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Thanks to all who responded to my query. We had already done most of the
things that were recommended. Here's an update and the apparent resolution:

According to her ophthalmologist, "Nancy's uncorrected vision has a focal
point of around 6 inches. Therefore, the reticle is perfectly in focus when
viewing the acellular field in the left eye, while the right does not have
an image to focus on." She suggested that Nancy use corrected vision when
using the microscope (e.g., contacts, implants, eyeglasses). In the mean
time, I have installed a modified screening reticle that retains the
outlined clear square area, but no longer includes the central circle and
crossed line size comparators.

Nancy reports that the "blurring" she previously experienced has now largely
gone away. In addition, she tried wearing her eyeglasses and found that the
problem has disappeared entirely. She's loathe to wear her eyeglasses
during microscopy, however, as they cost $400 and she doesn't want to
scratch the lenses -- ocular eye guards notwithstanding.

Gary Gill


From daemon Tue May 22 20:19:47 2001



From: Susan Belfry :      belfry-at-unb.ca
Date: Tue, 22 May 2001 20:17:40 -0500
Subject: MSC2001 Conference - Last Update

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Fellow Microscopists,

The scientific programme for the Annual General Meeting of the
Microscopical Society of Canada (June 6-8, 2001, Fredericton, NB) is
established and is available at the website
{http://www.unb.ca/msc2001} . Please, check the programme for a list
of the topics to be presented and also note that all of the major
microscopy vendors will be present to demonstrate the latest
developments in confocal, light and electron microscopes.
This will be the last update to the conference website.

Susan Belfry


From daemon Wed May 23 08:50:05 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Wed, 23 May 2001 09:41:47 -0400
Subject: Microspheres

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Hello Listers,
I would like to thank all of you who replied with help on finding the
microspheres. I would have individually emailed those who responded, but due
to a network hiccup, I lost all my email. I was fortunate enough to have
hard copies of where to find some things, but no email of who to thank for
the information.
Have a good day,
Nick


From daemon Wed May 23 09:19:09 2001



From: COURYHOUSE-at-aol.com
Date: Wed, 23 May 2001 10:14:20 EDT
Subject: Re: Source of used 4X objective

Contents Retrieved from Microscopy Listserver Archives
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--part1_64.e416398.283d1fbc_boundary
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the same obj for a 60 or a 50 will also work. they are all the ao infinity
series

ed sharpe archvisit for smecc

} Subj: Source of used 4X objective
} Date: 5/22/01 11:41:30 PM US Mountain Standard Time
} From: garygill-at-dcla.com (Gary Gill)
} To: Microscopy-at-sparc5.microscopy.com
}
}
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone point me to a source for such an objective for an old AO
} Microstar Series 110 microscope? I tried eBay once, but the objective
} didn't match the microscope requirements. Thanks.
}
}



--part1_64.e416398.283d1fbc_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit

{HTML} {FONT FACE=arial,helvetica} {FONT SIZE=2} the same obj for a  60 or a 50 will also work. they are all the ao infinity
{BR} series
{BR}
{BR} ed sharpe archvisit for smecc
{BR}
{BR} {BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"} Subj: {B} Source of used 4X objective {/B}
{BR} Date: 5/22/01 11:41:30 PM US Mountain Standard Time
{BR} {I} From:    garygill-at-dcla.com (Gary Gill)
{BR} To:    Microscopy-at-sparc5.microscopy.com
{BR} {/I}
{BR}
{BR}
{BR}
{BR} ------------------------------------------------------------------------
{BR} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
{BR} To  Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
{BR} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{BR} -----------------------------------------------------------------------.
{BR}
{BR}
{BR} Can anyone point me to a source for such an objective for an old AO
{BR} Microstar Series 110 microscope?  I tried eBay once, but the objective
{BR} didn't match the microscope requirements.  Thanks.
{BR}
{BR} Gary Gill {/BLOCKQUOTE}
{BR}
{BR} {/FONT} {/HTML}

--part1_64.e416398.283d1fbc_boundary--


From daemon Wed May 23 13:19:47 2001



From: Richard Portman :      richard-portman-at-utulsa.edu
Date: Wed, 23 May 2001 13:11:35 -0500
Subject: Argon Purity

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I may need to replace my Argon tank on my sputter coater soon. What is
the minimum purity of argon that you would recommend for use in an old
Emscope SC500A sputter coater?

Thanks in Advance

Rick




Richard Portman
Dep't. Biological Science
University of Tulsa
600 S. College
Tulsa OK 74104

(918)631-3715


From daemon Wed May 23 13:29:04 2001



From: Debbie Lietz :      dlietz-at-trentu.ca
Date: Wed, 23 May 2001 14:24:10 -0400
Subject: TEM-used Philips 301

Contents Retrieved from Microscopy Listserver Archives
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I have a Philips 301 microscope that needs a new home. It is in good
running condition. There are a lot of spare parts and consumables to go
along with the microscope. The mercury pump has been removed. The
microscope is presently up and running but will soon need to be dismantled
to free up the room for another microscope. If interested please email me
directly.
Dismantling and shipping will be at the new owner's expense.

Debbie Lietz
Electron Microscopy Technologist
Biology Department, Trent University
1600 Westbank Drive
Peterborough, Ontario
K9J 7B8

Tel: (705)748-1011 ext.1486 *** Fax: (705) 748-1205 ***Email:
dlietz-at-trentu.ca



From daemon Wed May 23 14:13:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 23 May 2001 15:09:04 -0400
Subject: FW: Generation of in situ osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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I found this on the NET, and I hope Dr. Kiernan doesn't mind.

Re: "Disposal" of Osmium Tetroxide "Waste" From: "J. A. Kiernan" {jkiernan-at-julian.uwo.ca} ------------------------------------------------------------------------ On Thu, 23 Nov 2000, stephen asquith wrote: } disposal of this wonderful material but it still ... } ... containers full of osmium in alcohol or corn oil ... } ... what should be done with it then? Osmium tetroxide is indeed wonderful stuff. Os is a rare element, so disposal of used solutions should consist of recycling, not dumping, even though osmium compounds are not considered environmentally hazardous (Smith et al., 1978 Trace Metal in the Environment, vol 4. Ann Arbor Science Publishers). The colourless soluble toxic tetroxide is rapidly reduced by almost any kind of dirt to a black, insoluble dioxide, usually in a colloidal form that's readily dispersed by moving water if it isn't firmly stuck to the solid organic matter that brought about the reduction. If OsO4 slops are collected in alcohol, the os!
mium (now in the form of crude, harmless, insoluble osmium dioxide) can be reoxidized, purified, re-reduced to pure OsO2 and stored. OsO2 is easily re-oxidized to give a buffered solution of osmium tetroxide (2% or less). See J Microsc 113:77-82 (1978); the procedure does involve certain hazards, so it must be done carefully. Recovered OsO4 can also be used to make osmeth, which is a beautiful golden crystalline solid that contains osmium tetroxide complexed with methenamine (= hexamethylene tetramine or hexamine (Hanker et al., 1976 Histochemistry 49:263-291). It costs next to nothing to make your own osmeth from recycled OsO4, but osmeth is very expensive to buy. Osmeth does not emit osmic fumes. When it is dissolved (in DMF followed by dilution in an aqueous buffer) it becomes a dilute (0.25%) working osmium tetroxide solution. I can vouch for the excellence of home-made osmeth for post-osmication (for EM). It may also be OK as a primary fixative or for LM stainin!
g, but I haven't encountered (personally, by anecdote or in the literature) any use of osmeth other than for postosmication. Perhaps someone reading this message will put me right on this. Osmium tetroxide collected into vegetable oil could not be recycled by the simple method cited above, and the recovery methods used by chemists (which use apparatus etc not found in histology labs) would be made more difficult by the presence of the oil. John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Beauregard, Paul A.
} Sent: Tuesday, May 22, 2001 4:02 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Generation of in situ osmium tetroxide
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I do staining of TEM thin sections of microporous polymer systems, rubber
} compounds and polymer based automotive coatings.
}
} I use RuO4 mostly for staining and would like to switch to OsO4
} occasionally. I use a very safe procedure in which I generate very small
} amounts of RuO4 vapors in a petri dish in situ. This limits problems with
} amounts handled, safety and minimizes disposal.
}
} In the literature I see articles and also web sites that talk about using
} hot fuming nitric acid (impractical for me to keep heated), hot nitric
} acid (ditto) and hydrogen peroxide on "Osmium Compounds" or metal.
} Suggestions are made at some sites that osmium dioxide is used. OsO2 does
} not seem to be sold at any chemical supply houses nor is it in any EM
} supplier catalogs I have.
}
} I DO NOT want to use ampoules of OsO4 or RuO4 and have them stored around
} the lab in sealed vials. I had an unscored ampoule of RuO4 spontaneously
} break once and it was not pleasant for my refrig' or me.
}
} Question(s):
} Have you ever experimented with or know of any osmium compound(s) used to
} in situ generate osmium tetroxide vapors from one of these compounds?
} Is the procedure safe and does it generate small amounts of waste?
} What do you use as a staining indicator?
} Butadiene rubber or a solution of it, works for me with OsO4.
}
} My aim here is not to find a 'cheap' way to make OsO4. Os costs a lot of
} money no matter what you buy or where.
}
} Thank you for any help you can give or ideas.
}
} Paul Beauregard
} PPG Industries
} Senior Research Associate
} Monroeville, PA 15146
}
}
}
}


From daemon Wed May 23 18:56:24 2001



From: Diana M Papoulias :      diana_papoulias-at-usgs.gov
Date: Wed, 23 May 2001 18:48:08 -0500
Subject: cadmium stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am seeking assistance in finding a method to stain cadmium in paraffin
embedd tissue. If such a method exists, I would very much appreciate your
help.

Thank you in advance,

Diana Papoulias
Fisheries Biologist, Research
US Geological Survey
Columbia Environmental Research Center
4200 New Haven Rd.
Columbia, Missouri 65201

voice mail: 573-876-1902
e-mail: Diana_Papoulias-at-usgs.gov
fax: 573-876-1896



From daemon Wed May 23 19:29:23 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 23 May 2001 17:31:44 -0700
Subject: Re: Argon Purity

Contents Retrieved from Microscopy Listserver Archives
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I use cheap, industrial grade Argon which is run through
a Millipore molecular sieve. The sieve lasts for years.
The cylinder of Ar costs about $20 and also lasts for years.
The sieve assembly cost about $300. Virtually a one time
expense.

I do the same for N2. I use industrial grade N2 ($20 per cylinder
rather than 9.8 N2 ($90 per cylinder). After two years, the
N2 sieve is unchanged.

gary g.



At 11:11 AM 5/23/2001, you wrote:

} Hi,
}
} I may need to replace my Argon tank on my sputter coater
} soon. What is
} the minimum purity of argon that you would recommend for use in an old
} Emscope SC500A sputter coater?
}
} Thanks in Advance
}
} Rick
}
}
}
}
} Richard Portman
} Dep't. Biological Science
} University of Tulsa
} 600 S. College
} Tulsa OK 74104
}
} (918)631-3715



From daemon Thu May 24 09:45:01 2001



From: JaneCPoke-at-aol.com
Date: Thu, 24 May 2001 10:30:09 EDT
Subject: Photos

Contents Retrieved from Microscopy Listserver Archives
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I am a photo editor and photo researcher for most texts in biology, zoology,
botany, microbiology, genetics, anatomy & physiology, etc., published and I'm
always searching for new sources of images to make texts more interesting,
attractive, and educational. Dr. John D. Cunningham, VU Research, Inc., 46
The Flume, Amherst, NH 03031 Fax: 603-673-4738 email:
john-at-visualsunlimited.com


From daemon Thu May 24 17:38:47 2001



From: ikewlee-at-yahoo.com
Date: Thu, 24 May 2001 17:33:01 -0500
Subject: Ask-A-Microscopist: DAPI (Ex 364nm; Em 454nm) to trace cells

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(ikewlee-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
May 24, 2001 at 10:17:30
---------------------------------------------------------------------------

Email: ikewlee-at-yahoo.com
Name: Ike W. Lee

Organization: Anterogen

Education: Graduate College

Location: Roxbury, MA, USA

Question: Hi!
I am using DAPI (Ex 364nm; Em 454nm) to trace cells during cell
transplantation in animals. Two months after the transplantation when
I examined the DAPI positive tissue under a light microscope, it was
fluorescent not only with the DAPI filter (Ex 340-380nm; Em 420nm),
but also with the two other filters (Ex 465-495, Em515-555 and, Ex
525-555, Em 590-650). Before transplantation, the cells were
fluorescent with the DAPI filter only when it was checked in a tissue
flask (plastic). The tissue samples were simply frozen cut without
fixation. Narrow-spentrum filters of a confocal microscope were not
different; in other words, DAPI positive cells were fluorescent in
all confocal filters tested. My questions are:
(1). Is it possible that DAPI can change its wavelength in vivo after
two months?
(2). Is there a way to do immunohistochemistry on these weird
DAPI-positive tissue sections?
(3). Are there any other methods with which I can trace cells during
the two months of post-transplantation period?

Thank you.

Ike Lee
Anterogen
ikewlee-at-yahoo.com
(617) 442-7840, ext 246


---------------------------------------------------------------------------


From daemon Thu May 24 20:05:27 2001



From: Katjaalex-at-aol.com
Date: Thu, 24 May 2001 20:59:13 EDT
Subject: Jeol 1200 EX

Contents Retrieved from Microscopy Listserver Archives
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Hi, we have a problem with switching on the unit. If you turn the key, it
will start, but when you release the key, it stops immediately. The Forepump
does not start, we have enough air & water. All fuses check OK. Any ideas?
Thank you!
Peter Stolzenberg


From daemon Thu May 24 20:52:23 2001



From: sae-49-at-webtv.net
Date: Thu, 24 May 2001 21:47:57 -0400 (EDT)
Subject: Microsopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I live in NY HOW can I have my blood done by this technique? Please let
me Know !






From daemon Fri May 25 02:18:09 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 25 May 2001 08:14:48 +0100 (GMT Daylight Time)
Subject: Re: Jeol 1200 EX

Contents Retrieved from Microscopy Listserver Archives
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Hi Peter,

} From memory if the instrument switches off when you release
the key then the rotary pump is not running properly.
You say the rotary pump does not start - do you mean that
it does not start at all? If not then that is your problem
- do you have power to it when the key is operated? If not
then check the circuits for fuses and breakers. If so then
you have a faulty motor. If the motor runs but switches off
with the key - is the pump up to speed (belt slipping)? If
not then there is usually a pump rotation sensor which will
detect a pump fault. Has the rotation detector been
knocked? Try moving it closer to the flywheel.

I don't know where you are (no affliation attached) but our
local JEOL people are very good at offering advice.

Good luck,
Ron

On Thu, 24 May 2001 20:59:13 EDT
"Katjaalex-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, we have a problem with switching on the unit. If you turn the key, it
} will start, but when you release the key, it stops immediately. The Forepump
} does not start, we have enough air & water. All fuses check OK. Any ideas?
} Thank you!
} Peter Stolzenberg
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri May 25 08:43:10 2001



From: Ken Bart :      kbart-at-hamilton.edu
Date: Fri, 25 May 2001 09:39:54 -0500
Subject: Re: Jeol 1200 EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Hi, we have a problem with switching on the unit. If you turn the key, it
} will start, but when you release the key, it stops immediately. The Forepump
} does not start, we have enough air & water. All fuses check OK. Any ideas?
} Thank you!
} Peter Stolzenberg

Peter:

I often have the same problem with my 1200EX II. Sometimes
this is caused by a misalignment of the flywheel sensor on the
mechanical pump. Other times it is due to insufficient air pressure.
Try this: turn the power off at the main disconnect, then switch it
back on; set the the key to the ON position for a few minutes before
moving it to START. You may have to repeat this cycle a few times,
but it always works on my instrument. Good Luck!

Ken Bart

.....................................................................
.....................................................................
............
Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu


From daemon Fri May 25 13:32:00 2001



From: Yali Tang :      ytang-at-anl.gov
Date: Fri, 25 May 2001 13:19:30 -0500
Subject: Re: Jeol 1200 EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1. when starting, it is suggested you need to hold the key for a while, then
release,
2. check the belt of the pump
3. check the water again, a sensor connect to oil pump will stop the machine
when no water running

Yali
----------
} From: Ron Doole {ron.doole-at-materials.oxford.ac.uk}
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Jeol 1200 EX
} Date: Fri, May 25, 2001, 2:14
}

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri May 25 14:19:48 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 May 2001 12:21:28 -0700
Subject: Re: Argon Purity

Contents Retrieved from Microscopy Listserver Archives
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I got the maker wrong. It is Matheson Gas Products, not
Millipore.

The Matheson Glass Moisture Trap (with pre-loaded sieve
material) is P/N 6420-4S. The Glass Moisture Refill kit
(several plastic containers of sieve material) is P/N 6420-R.

Any outfit which handles Matheson should be able to order
these items for you. The sieve unit has stainless steel
Swagelok fittings on each end. A really nice product.
Zero maintenance.

gary g.


At 03:39 AM 5/25/2001, you wrote:

} Hi, Gary
}
} Can u advise the details of the Millipore thingy?
}
} ta
}
} rtch
}
}
} }
} } I use cheap, industrial grade Argon which is run through
} } a Millipore molecular sieve. The sieve lasts for years.
} } The cylinder of Ar costs about $20 and also lasts for years.
} } The sieve assembly cost about $300. Virtually a one time
} } expense.
} }
} } I do the same for N2. I use industrial grade N2 ($20 per cylinder
} } rather than 9.8 N2 ($90 per cylinder). After two years, the N2
} } sieve is unchanged.
} }
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Fri May 25 14:27:13 2001



From: Philip Flaitz :      flaitz-at-US.ibm.com
Date: Fri, 25 May 2001 15:26:11 -0400
Subject: Re: Etching of ZnSe.

Contents Retrieved from Microscopy Listserver Archives
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We have a sample of ZnSe with a thin layer of SiO2 on the surface. We
would like to remove the SiO2 without damaging the ZnSe. Does anybody on
the list know if ZnSe is susceptible to attack by HF? It has been
suggested to me that this is possible and I was wondering if anyone has any
practical experience to confirm or deny the suggestion.


Phil Flaitz
IBM Microelectronics, Hopewell Junction, NY
Ph.......(845) 892-3094, FAX -2003
pager - 800-352-4732, PIN# 1121
flaitz-at-us.ibm.com



From daemon Fri May 25 15:06:27 2001



From: Rehorek, Susan S. :      susan.rehorek-at-sru.edu
Date: Fri, 25 May 2001 16:03:59 -0700
Subject: Histoembedder

Contents Retrieved from Microscopy Listserver Archives
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G'day,

I am trying to locate a histoembedder: for paraffin histology. The only
one I have thus far been able to locate is one for $8500 - with way to many
swanky things on it (to tell me the precices temperature of the paraffin,
and under vacuum). (I don't need that - and I can't justify the school
buying that kinda equipment for me). I remember using simple one -
paraffin on one end, and the cold plate on the other - with only two
buttons (the fewer the better, especially since this will be used in
teaching undergrads).

Does anyone out there know where I can get such a machine for a reasonable
price?
Susan J Rehorek, Ph.D.
Department of Biology
Slippery Rock University of Pennsylvania
PA, 16057-1326

ph: 724 738 2485
fax: 724 738 4782
email: susan.rehorek-at-sru.edu


From daemon Fri May 25 16:16:20 2001



From: Axel Blau :      axel-at-caltech.edu
Date: Fri, 25 May 2001 14:08:48 -0700
Subject: Seeking a high N.A. water-immersion lens

Contents Retrieved from Microscopy Listserver Archives
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We are looking for a microscopy objective that matches the following features:

- high N.A. (} 0.9),
- water-immersion,
- slim diameter (preferably { 0.7 inches or {18 mm at its end),
- any magnification between 20x and 63x,
- corrected for 160 mm (Nikon Measurescope MM-11),
- suitable for fluorescence imaging,
- preferably with a large working distance (for electrophysiology).

The lens can be new or used. Maybe you have something like that collecting dust
on your shelves. Any offer is welcome.

Thanks a lot, with best regards,

Axel



Axel Blau, Ph.D.
California Institute of Technology
Division of Biology 156-29
1200 E. California Blvd.
Pasadena, CA 91125

(626) 395-6787 phone
(626) 564-8709 fax

http://wecoyote.caltech.edu/~axelb/
http://www.caltech.edu/~pinelab/pinelab.html




From daemon Fri May 25 16:27:20 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Fri, 25 May 2001 17:22:56 -0400
Subject: Argon Purity

Contents Retrieved from Microscopy Listserver Archives
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Hi

Why so much fuss over the argon? I was involved in the development of some
of the early sputter coaters and argon was the least of our worries. We
often used air when making test runs! The only problem with air is one day
it may be dry the next day it may be wet so the coatings are different,
well in England any way!

If some one can PROVE a difference I would be very interested to hear? If
there is a problem, with different grades of argon I would be rather
surprised?

Regards looking forward to some informative replies :-)

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Fri May 25 17:38:00 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 25 May 2001 18:33:20 -0500
Subject: SiO2 film removal

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Phil Flaitz wrote:
==============================================
We have a sample of ZnSe with a thin layer of SiO2 on the surface. We would
like to remove the SiO2 without damaging the ZnSe. Does anybody on the list
know if ZnSe is susceptible to attack by HF? It has been suggested to me
that this is possible and I was wondering if anyone has any practical
experience to confirm or deny the suggestion.
===============================================

Why could the SiO2 layer not be etched off with a reactive CF4 gas, such as
CF4, using plasma etching? I would imagine that once the SiO2 layer was
etched off, it would continue to etch the substrate, so some kind of control
samples might have to be run to determine the exact time needed to remove
the SiO2 but not etch the substrate. If one back fills to the same vacuum
before starting, it is possible to get a remarkably reproducible etch on
successive samples.

Disclaimer: SPI Supplies manufactures the Plasma Prep II plasma etcher, a
full description of which can be found on our website indicated below.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================








From daemon Fri May 25 18:18:31 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 May 2001 16:20:10 -0700
Subject: Re: Amray FE filament replacement

Contents Retrieved from Microscopy Listserver Archives
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The short answer is NO. But here is what I do know about
the 305FE gun--after about two years of experience on two
different FE scopes.

So I'm told, the filament is a ZrO/W unit made by Denka.
The top of the filament holder is held in place by a zillion
Allen head bolts. Also on the top holder plate are three pairs
of large gauge wires which are the leads for the three "getters."

When the filament is replaced (factory), there is some sort of alignment
procedure. This involves three small Allen head set screws on
the top sides of the gun housing. Once aligned, these
screws are supposedly never adjusted again. But, if during a
move, and the filament assembly got really whacked out of
position, it is re-aligned using these set screws. There is probably
a lot more work for filament replacement than just replacing
the filament. The whole electrode assembly may be cleaned
or replaced (extractor, etc.).

The construction of the gun assembly
is such that there is a gun isolation valve (V1) which closes off
the gun from the lower column. At the top of the column is a
differential pumping aperture. This is not a consumable item.
Below the DPA is the column isolation valve (V3). Some of these
two valves are pneumatic while others (like mine) are manual.

As-shipped, the FE gun includes a Varian 1 L/second ion pump.
The gun is under high vacuum at about 8E-11 Torr. Replacement
of the gun is not simply replacement of the gun assembly. It
involves "conditioning" the new gun as-received via a lengthy
process. The gun is well grounded to earth ground. Then, the
ion pump is powered by a small power supply which provides
a voltage output proportional to ion pump current. Then, the
whole gun head assembly (high tension cable for all electrodes)
are slowly raised to 32KV. When this is being done, the
IPG (gun ion pump current) is monitored. If it starts to increase,
the KV is reduced, held and then slowly increased. This process
continues until 32KV is reached. If at any point, the gun head
arcs, the whole process starts over. It seems to take about three
hours to complete this process.

Once the conditioning is done, the gun assembly is mounted to
the column. Then, vigorous pumping begins. The filament is
off and V1 is still closed. Once the column vacuum reaches
a safe level (typically 5E-10 Torr), the gun isolation valve is
opened, vacuum checked, and the filament can be heated. It takes quite a few
days for the filament to stabilize (Iext will vary for a while).
Once stable, it remains very stable until it dies. Handled
correctly, a gun is good for anywhere between two to four years.

Power failure, poisoning of the filament and surges can cause
premature failure of the filament/gun. Right now, I am told
that Amray is supporting all FE systems. The 305FE gun is
the same one used on the KLA-Tencor semiconductor defect
analysis tools. It is a very good gun. Extraordinarily stable.
I use two double conversion UPS units (Toshiba 1400XL Plus)
to protect the column and console assemblies. This helps a
lot....especially here in California these days!

Under a maintenance contract, the gun is a $2K consumable.
Without a contract, it is a $16K item. So I am told. I do know
that it costs $2K to replace under contract since I have had
to have that done one time.

gary g.




At 07:25 PM 5/21/2001, you wrote:

} Dear Listers,
}
} Has anyone sucessfully done an Amray FE filament replacement for the newer
} "305" guns?
}
} I think there is more to it than meets the eye.
}
} Thank You,
}
} Earl



From daemon Fri May 25 18:26:32 2001



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Fri, 25 May 2001 18:24:47 -0500
Subject: Balzers Turbo Pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
It appears that Pfeiffer(Balzers) Vacuum Company will no longer
be supporting its older turbo pumps, like the TPH 170, 240, etc. No
parts, no repairs, nothing. I received notification from Pfeiffer
that this will be effective Jan. 1, 2002. Of course, they want you
to buy their "new" pumps. All of the independent turbo repair shops
are scrambling trying to find parts for the pumps they are repairing
now, or have commitments to repair in the future. Naturally, the
cost of repairs to these pumps has skyrocketed. The attraction of
the use of Balzers turbo pumps in electron microscopes was the fact
that this pump was in effect "self-balancing", emitting very low
mechanical vibrations. Has anyone found any suitable replacement for
this pump by another manufacturer? Suitable meaning not only good
vibration specs but also reasonable in cost, for purchase and or to
repair.



Gary M. Easton
Scanners Corporation


From daemon Fri May 25 18:27:17 2001



From: JHoffpa464-at-aol.com
Date: Fri, 25 May 2001 18:25:35 -0500
Subject: subsitiute for glut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi all someone posed a question to me after being inspected by JCAHO the
} inspectors were upset they were using glut. i had never heard of a good
} subsitiute for glut so i thought i would throw it out to you guys. he
didn't
} say if it was a labeling problem or just the fact they had glut. i have
never
} heard of such a thing before.
} john hoffpauir
} electron microscopy lab
} cooper hospital camden nj


From daemon Fri May 25 18:27:42 2001



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Fri, 25 May 2001 18:25:59 -0500
Subject: ISI SR50A SEM

Contents Retrieved from Microscopy Listserver Archives
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Listers,
Anyone having a image of the ISI SR50A SEM(like from an old sales
brochure or pic from a digital camera) that could emailed to me, it
would be greatly appreciated. Please send to
{mailto:gary.easton-at-scannerscorp.com} gary.easton-at-scannerscorp.com
directly. Thanks in advance.


Gary M. Easton
Scanners Corporation


From daemon Fri May 25 18:29:05 2001



From: Julie Piraino :      piraino-at-sms.si.edu
Date: Fri, 25 May 2001 18:27:06 -0500
Subject: Confocal Information Request - USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I am located on the central Atlantic coast of Florida and am
considering purchase of a confocal microscope from BioRad. I would
appreciate hearing, offline, from anyone in the southeastern US who
has had first-hand experience (good or bad) with their equipment and
service.

Please respond to: piraino-at-sms.si.edu

Thank You.

Julie Piraino


From daemon Fri May 25 19:33:01 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 May 2001 17:33:52 -0700
Subject: Re: Etching of ZnSe.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about a plasma etch with CF4 + O2? That will etch the
SiO2. Not sure about the other materials. You might want to
run some tests on this.

gary g.


At 12:26 PM 5/25/2001, you wrote:

} We have a sample of ZnSe with a thin layer of SiO2 on the surface. We
} would like to remove the SiO2 without damaging the ZnSe. Does anybody on
} the list know if ZnSe is susceptible to attack by HF? It has been
} suggested to me that this is possible and I was wondering if anyone has any
} practical experience to confirm or deny the suggestion.
}
}
} Phil Flaitz
} IBM Microelectronics, Hopewell Junction, NY
} Ph.......(845) 892-3094, FAX -2003
} pager - 800-352-4732, PIN# 1121
} flaitz-at-us.ibm.com



From daemon Fri May 25 20:35:41 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 25 May 2001 18:33:20 -0500
Subject: SiO2 film removal

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Phil Flaitz wrote:
==============================================
We have a sample of ZnSe with a thin layer of SiO2 on the surface. We would
like to remove the SiO2 without damaging the ZnSe. Does anybody on the list
know if ZnSe is susceptible to attack by HF? It has been suggested to me
that this is possible and I was wondering if anyone has any practical
experience to confirm or deny the suggestion.
===============================================

Why could the SiO2 layer not be etched off with a reactive CF4 gas, such as
CF4, using plasma etching? I would imagine that once the SiO2 layer was
etched off, it would continue to etch the substrate, so some kind of control
samples might have to be run to determine the exact time needed to remove
the SiO2 but not etch the substrate. If one back fills to the same vacuum
before starting, it is possible to get a remarkably reproducible etch on
successive samples.

Disclaimer: SPI Supplies manufactures the Plasma Prep II plasma etcher, a
full description of which can be found on our website indicated below.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================












From daemon Fri May 25 22:35:09 2001



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 25 May 2001 23:39:16 -0400
Subject: final reminder...ASM symposium...

Contents Retrieved from Microscopy Listserver Archives
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Gary,

I wasn't so much concerned with the conditioning of the filament.

This process is fairly straightforward. Amray brings up the high voltage
until the gun arcs causing the vacuum to deteriorate. The heaters
(non-evaporative getters) are again activated to bring the pressure down
again. This process is repeated until a maximum voltage of 35 KV for one
hour is achieved.
The external filament adjustments are two assemblies: one for X another for
Y.
These adjustments push on two glass rods that are internally spring loaded.
The assemblies are composed of a stainless steel "cup" , a brass adjustment
pin, & a small (looks like a 4-40) setscrew.
The real adjustment is made on the brass pin only; the setscrew is used to
limit the brass pin travel only.

I am more concerned with the initial mechanical pre-alignment of the
filament itself.
It appears that the filaments as sold by Denka and/or FEI come pre-aligned
in it's own wehnelt assembly.
This entire assembly is held in yet another "sleeve" that has another four
setscrews to align it again. Seems redundant but perhaps there is a reason.

Another interesting note is that the filament construction appears to be
different between the Denka & FEI filaments.
This is according to their respective websites. Also the price difference is
significant: FEI price is $1750.00 while Denka is $2600.00.
Amray has used both vendors.

Another interesting detail is that the manufacturer's recommend that the
filaments be operated at 1800 deg K.
Amray runs the filaments at about 1600-1650 deg K & raises the extraction
voltage to compensate.
I think this would give the filament more life at the cost of some
stability.


By the way, I believe there are 16 allen head screws on the top of the gun
housing & the appendage pump is a 2 l/s pump.

Best Regards,

Earl


----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Earl Weltmer" {earlw-at-pacbell.net}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, May 25, 2001 4:20 PM


Just a reminder to Listers who might be interested to attend the ASM
Educational Symposium "Materials MicroCharacterization: Today and
Tomorrow" next week in Oak Ridge...please check out details at
http://www.korrnet.org/orcasm/. We're especially interested to have
students attend, and the registration is only $20 (for students).

Larry

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Sat May 26 01:28:21 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 26 May 2001 16:24:43 +1000
Subject: RE: substitute for glut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Except for microwave fixation, which is not universally accepted, all fixatives
are nasty. After all, fixatives are meant to kill cells and tissues. Coming to
think of it: microwaves are nasty too, except that they are contained within
the oven.
And that is the take-home message for the safety officer: Any substance can be
used in great safety if the correct procedures and facilities are applied. The
right facilities and procedures for some substances can be too expensive, but
not for the use of modest quantities of GA as in EM. A fumehood and suitable
(Nitrile) gloves and fairly elementary working skills make the use of GA very
save.

Where are the cases of EM related problems with GA? The few that have occurred
were caused by bad facilities/ working habits or related to a sensitivity
problem.

I expect that GA has a bad name in hospitals because of its use as a
sterilising agent. Rooms have been sterilized by spraying GA as an aerosol and
operating instruments have been bathed in large trays of GA, at times in
unventilated rooms. EM people don't have such absurd applications.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, May 26, 2001 9:26 AM, "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
[SMTP:"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hi all someone posed a question to me after being inspected by JCAHO the
} } inspectors were upset they were using glut. i had never heard of a good
} } subsitiute for glut so i thought i would throw it out to you guys. he
} didn't
} } say if it was a labeling problem or just the fact they had glut. i have
} never
} } heard of such a thing before.
} } john hoffpauir
} } electron microscopy lab
} } cooper hospital camden nj


From daemon Sat May 26 04:09:00 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sat, 26 May 2001 02:02:57 -0700
Subject: Re: Balzers Turbo Pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EDWARDS distributes SEIKO SEIKI magnetically levitated (active magnetic
suspension of both ends) turbopumps. I am using STM-450 (450 l/sec) for 2
years and really happy with this stuff. Extremely easy to operate (actually
stop/start button). Don't need any cooling (even air flow). Any
orientation. And supposed to be completely vibration-free, because the
rotor have no contact with "body". In case of emergency stop it uses
internal electricity generator to power controller and suspension. Active
suspension protects from any disbalance in the system. Does not require
any technical assistance. These pumps are pricely, but EDWARDS kindly
offered to me very good discount. These pumps are work-horses in industry
but rare in EM community. I have no idea why. Please, let me know if you
need more details.

No commercial interest, just happy user and owner.

Sergey

At 06:24 PM 5/25/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sat May 26 08:13:24 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Sat, 26 May 2001 08:09:38 -0500
Subject: Olympus accessories

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues

Does anyone have phase contrast and/or Nomarski optics for a
1970's Olympus Vanox microscope that they would like to sell or
give away? If so please contact me.

Regards




Rob Cross
Director : EM Unit, Rhodes University


Rob Cross
Director : EM Unit, Rhodes University


From daemon Sat May 26 08:16:15 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sat, 26 May 2001 09:13:39 -0400
Subject: LM: NYMS Workshop on use of the Microscope

Contents Retrieved from Microscopy Listserver Archives
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New York Microscopical Society
30 North Mountain Avenue
Montclair, NJ 07042


Bernard Friedman
Memorial Workshop



Use of the Microscope
September 15, 22, 29, October 6, 2001

A basic course on light microscopy which will cover the following topics:
Theory of microscopy
Kohler Illumination
Diffraction Theory
Contrast Methods
Polarized light
Phase Contrast
Interference
. . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination,
etc.

The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of microscopy.
The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of
Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR
Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHEN: September 15, 22, 29, October 6, 2001 from 10 A.M. to 4 P.M.

WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 (
parking, accessible by public transportation, Information on car pools and
transportation will be provided.)

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Beginners and experienced users who wish to learn more about the
proper use of a microscope.

HOW: Register using the form below. Limited to the first 12 registrants.
Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849
E-mail donoleary-at-worldnet.att.net Fax (425) 988-1415

PLEASE POST
----------------------------------------------------------------------------
-------------------
Registration Form
Use of the Microscope

N.Y.M.S. Member_________________ ($275) Non-Member__________($295)

Name______________________________________________________________________
Address____________________________________________________________________
Phone (W)_______________________(H)___________________________



From daemon Sat May 26 09:20:27 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 26 May 2001 07:22:12 -0700
Subject: Re: Balzers Turbo Pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ISI/Topcon uses/used these pumps too. They are very nice and quite
reliable. I think the reason the masses don't use them is because they
are about four times more costly than the single mag bearing pumps
like the Balzers. If they got close to the cost of the Balzers, they
certainly would be strong contenders for replacement units. The down
side is the re-wiring it would take to incorporate the Seiko units.
Most of the controllers are much larger than those for the Balzers.
And the in and out signals may be different.

gary g.


At 02:02 AM 5/26/2001, you wrote:

} EDWARDS distributes SEIKO SEIKI magnetically levitated (active magnetic
} suspension of both ends) turbopumps. I am using STM-450 (450 l/sec) for 2
} years and really happy with this stuff. Extremely easy to operate
} (actually stop/start button). Don't need any cooling (even air flow). Any
} orientation. And supposed to be completely vibration-free, because the
} rotor have no contact with "body". In case of emergency stop it uses
} internal electricity generator to power controller and suspension. Active
} suspension protects from any disbalance in the system. Does not require
} any technical assistance. These pumps are pricely, but EDWARDS kindly
} offered to me very good discount. These pumps are work-horses in industry
} but rare in EM community. I have no idea why. Please, let me know if you
} need more details.
}
} No commercial interest, just happy user and owner.
}
} Sergey
}
} At 06:24 PM 5/25/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat May 26 10:10:15 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 26 May 2001 08:13:32 -0700
Subject: Re: Etching of ZnSe.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Plasma etching of SiO2 with only CF4 is rather slow. The optimum mix
of O2 is 10%.

Just thinking here, but you might also try Ammonium Flouride.
It is a selective oxide etch and will not attack metal. At least it
will not attack Aluminum. HF will attack Al. You can obtain
BOE (buffered oxide etch) which has or does not have HF.
This may work too. The wet etch methods may be much
easier to accomplish than plasma, if you are not already
set up the work and expense involved with plasma.

gary g.


At 12:26 PM 5/25/2001, you wrote:

} We have a sample of ZnSe with a thin layer of SiO2 on the surface. We
} would like to remove the SiO2 without damaging the ZnSe. Does anybody on
} the list know if ZnSe is susceptible to attack by HF? It has been
} suggested to me that this is possible and I was wondering if anyone has any
} practical experience to confirm or deny the suggestion.
}
}
} Phil Flaitz
} IBM Microelectronics, Hopewell Junction, NY
} Ph.......(845) 892-3094, FAX -2003
} pager - 800-352-4732, PIN# 1121
} flaitz-at-us.ibm.com



From daemon Sat May 26 10:37:42 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 26 May 2001 08:39:40 -0700
Subject: Re: Amray FE filament replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:25 PM 5/25/2001, you wrote:
} Gary,
}
} I wasn't so much concerned with the conditioning of the filament.
}
} This process is fairly straightforward. Amray brings up the high voltage
} until the gun arcs causing the vacuum to deteriorate. The heaters
} (non-evaporative getters) are again activated to bring the pressure down
} again. This process is repeated until a maximum voltage of 35 KV for one
} hour is achieved.

Yes. Has to be done right or the gun will never perform correctly.

} [snip]
}
} Another interesting note is that the filament construction appears to be
} different between the Denka & FEI filaments.
} This is according to their respective websites. Also the price difference is
} significant: FEI price is $1750.00 while Denka is $2600.00.
} Amray has used both vendors.

I had heard that FEI may have supplied filaments. Lately, Denka
seems to be more predominant. No proof of which is used more or
less or not at all.


} Another interesting detail is that the manufacturer's recommend that the
} filaments be operated at 1800 deg K.
} Amray runs the filaments at about 1600-1650 deg K & raises the extraction
} voltage to compensate.
} I think this would give the filament more life at the cost of some
} stability.

Each new gun comes with a comprehensive spec sheet with all
parameters specified. The specs and values are taken at some
particular filament current, based on each individual filament/gun.
The standard practice is to reduce this current by 70mA. That is
then the continuous filament current for the gun. The Vext is
then adjusted to meet the gun brightness spec of 12.5nA. This
takes a while (several days) to settle in on. Iext is more of a
notional indicator of gun condition. It may vary a bit over time (and does
vary absolutely as-delivered from gun to gun) but is quite stable
until the gun starts to die. Conversely, the gun brightness value
is very stable. It may vary by no more than 0.5nA or less each month.

As part of my routine qualification procedure, I take all gun status
readings and the gun brightness value. A healthy gun/filament
barely varies in gun brightness. It is very stable. I think the
reduction in filament current was to extend the life of the filament.


} By the way, I believe there are 16 allen head screws on the top of the gun
} housing & the appendage pump is a 2 l/s pump.

Ah...that explains it. I could not count all of the screws using fingers
on both hands. Thanks.

Perhaps it is indeed a 2L/s pump. Still, it is a tiny little ion
pump. But critical.

gary



From daemon Sat May 26 16:00:53 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 26 May 2001 14:46:30 -0700
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Seiko pumps are not made to withstand the constant cycling from
atmosphere to high vacuum that the Balzers pumps will endure.

ISI/ Topcon has valving assembly which isolates the turbo from this cycling.
the Cambridge/ Leo & later Amray pump and vent the entire pumping system
each time a sample is introduced.



Earl

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, May 26, 2001 7:22 AM


Retail boxed Linux (Redhat, for example) is not free. It is about
the same price as Win2K.

The main problem is as you point out--application programs. While
many are available for Wintel, there are few for Linux. Being an
open system, Linux depends on having source code so one can
compile the app for the specific Linux platform. This is unlikely to
happen for industrial strength apps. They do not supply source code.
And most probably, they do not support Linux.

Nevertheless, Linux with X-windows on a P-III/733 or 933 is really awesome!

gary g.


At 08:33 PM 5/21/2001, you wrote:

} I would agree that familiarity with the operating environment is important
} for timely performance of server administration tasks-documentation and
} advocacy groups exist for both platforms, however. It might be worth while
} to become familiar with a UNIX like operating system (such as Linux) if one
} has the luxury of time (I'll explain momentarily). Cost may be an
} issue-Linux is free. Both OSes run on the x86 architecture.
} It seems that your choice(s) of application software should be a
} heavywieght factor in your decision. At the risk of over-simplification
} I'll just say that most proprietary "out of the box" solutions are developed
} for Windows/NT. There is a huge installed base of Wintel machines, and
} there are powerful software development tools for this platform which allow
} software developers to develop large complicated programs very rapidly.
} Linux has the advantage that many programs written to run under the
} various flavors of UNIX will compile and run. Many cutting edge scientific
} applications, including image processing utilities, are developed by
} researchers who are experienced coders and prefer to work under the UNIX
} platform. The reason for this is partially historical-in the past only large
} UNIX mainframe servers had the horsepower for image manipulation. More
} often than not these software tools are available free of charge to fellow
} researchers and are provided as open-source (one can view and modify the
} original code to suit specialized situations).
} So it really boils down to an ease of use vs. scalability/extensibility
} issue with cost, and perhaps minor hardware decisions (sometimes peripherals
} are difficult to configure under Linux ie. video cards, scanners etc...).
} In the long run, however, time spent now learning to work with Linux may pay
} off: the heart of the OS (the kernel) evolves continually in response to
} the needs of users, and the kernel can be upgraded without disrupting other
} parameters of the OS. Also, Linux runs on most (if not by now all)commonly
} encountered chip architectures so one may have several different kinds of
} machine (PC, Mac, SUN) all running the same operating system.
}
} Karl Garsha
} www.uwm.edu/~keg
} ----- Original Message -----
} } From: "Chao-Ying Ni" {cni-at-udel.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, May 18, 2001 9:36 AM
} Subject: EM Lab Server OS: Linux or Windows 2000?
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear colleagues,
} }
} } I'm going to build an EM lab server to run web and other applications,
} } to share files, especially to have an interactive wed scheduling software
} } to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I have
} } narrow down the platform to Linux and Windows 2000. Could you please give
} } me some inputs concerning the pros and cons of these two operating
} } systems? Any comments and suggestions on the hardware selection are also
} } more than welcome. Thanks in advance!
} }
} } Chaoying Ni
} } Electron Microscopy Lab
} } Dept. of Materials sci. and Eng.
} } University of Delaware
} } Newark, DE 19716
} } cni-at-udel.edu
} }
} }
} }
} }



From daemon Sun May 27 01:11:21 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 27 May 2001 01:02:13 -0500
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The question is about a server not a work station or PC. Very few
applications need to be run on a server and all the ones that are needed
are almost always included in the basic free distribution of Linux. Using
a server for any other use is asking for trouble. Just set it up and leave
it alone. I don't think anyone has actualy been at the console of my
server more than a dozen time in 5 years. I just telnet in and use vi. I
suppose if I had easy physical access to the box I would consider
installing X windows but it way too slow over the phone line.

Almost all the Linux distributions are coming with a installation program
that make it pretty simple to get things running. If you run a mailer it
is potentially the greatest problem. If you are going to run a mailer I
would seriously consider Linux or Unix because of the security issues with
windows. With Linux in particular the security problems are quickly
reported and the fixes quickly available. Who knows with windows.

Gordon
Who never found a mailer I liked.

Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
} From: "Gary Gaugler" {gary-at-gaugler.com}
}
} Retail boxed Linux (Redhat, for example) is not free. It is about
} the same price as Win2K.
}
} The main problem is as you point out--application programs. While
} many are available for Wintel, there are few for Linux. Being an
} open system, Linux depends on having source code so one can
} compile the app for the specific Linux platform. This is unlikely to
} happen for industrial strength apps. They do not supply source code.
} And most probably, they do not support Linux.
}
} Nevertheless, Linux with X-windows on a P-III/733 or 933 is really
awesome!
}
} gary g.
}
}
} At 08:33 PM 5/21/2001, you wrote:
}
} } I would agree that familiarity with the operating environment is
important
} } for timely performance of server administration tasks-documentation and
} } advocacy groups exist for both platforms, however. It might be worth
while
} } to become familiar with a UNIX like operating system (such as Linux) if
one
} } has the luxury of time (I'll explain momentarily). Cost may be an
} } issue-Linux is free. Both OSes run on the x86 architecture.
} } It seems that your choice(s) of application software should be a
} } heavywieght factor in your decision. At the risk of
over-simplification
} } I'll just say that most proprietary "out of the box" solutions are
developed
} } for Windows/NT. There is a huge installed base of Wintel machines, and
} } there are powerful software development tools for this platform which
allow
} } software developers to develop large complicated programs very rapidly.
} } Linux has the advantage that many programs written to run under
the
} } various flavors of UNIX will compile and run. Many cutting edge
scientific
} } applications, including image processing utilities, are developed by
} } researchers who are experienced coders and prefer to work under the
UNIX
} } platform. The reason for this is partially historical-in the past only
large
} } UNIX mainframe servers had the horsepower for image manipulation. More
} } often than not these software tools are available free of charge to
fellow
} } researchers and are provided as open-source (one can view and modify
the
} } original code to suit specialized situations).
} } So it really boils down to an ease of use vs.
scalability/extensibility
} } issue with cost, and perhaps minor hardware decisions (sometimes
peripherals
} } are difficult to configure under Linux ie. video cards, scanners
etc...).
} } In the long run, however, time spent now learning to work with Linux
may pay
} } off: the heart of the OS (the kernel) evolves continually in response
to
} } the needs of users, and the kernel can be upgraded without disrupting
other
} } parameters of the OS. Also, Linux runs on most (if not by now
all)commonly
} } encountered chip architectures so one may have several different kinds
of
} } machine (PC, Mac, SUN) all running the same operating system.
} }
} } Karl Garsha
} } www.uwm.edu/~keg
} } ----- Original Message -----
} } } From: "Chao-Ying Ni" {cni-at-udel.edu}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, May 18, 2001 9:36 AM
} } Subject: EM Lab Server OS: Linux or Windows 2000?
} }
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear colleagues,
} } }
} } } I'm going to build an EM lab server to run web and other
applications,
} } } to share files, especially to have an interactive wed scheduling
software
} } } to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I
have
} } } narrow down the platform to Linux and Windows 2000. Could you please
give
} } } me some inputs concerning the pros and cons of these two operating
} } } systems? Any comments and suggestions on the hardware selection are
also
} } } more than welcome. Thanks in advance!
} } }
} } } Chaoying Ni
} } } Electron Microscopy Lab
} } } Dept. of Materials sci. and Eng.
} } } University of Delaware
} } } Newark, DE 19716
} } } cni-at-udel.edu
} } }
} } }
} } }
} } }
}
}




From daemon Sun May 27 03:08:21 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 27 May 2001 01:01:49 -0700
Subject: Re: Balzers Turbo Pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl and Gary

Thanks for your reply. Ironically, I do use my SEIKO STM-450 for frequent
air/atmosphere cycles because it installed on my vacuum evaporator. When I
shop around different models (Varian, Pfeiffer and native EDWARDS) the
major criterion was the ability to handle frequent air/vacuum cycles (a
few per day). After many conversations and discussions with technical
support teams, the SEIKO was chosen. I don't remember details (if you need,
I could check), but it seems to me the pump was 1.5x more expensive than
Varian with equal productivity (before discount). As I mentioned, EDWARDS
give me a very nice price, so it was not so terrible expensive. Shopped
for the pump I was surprised to see how companies use TPs. For example, on
the Denton DV-502A 800 l/sec aca Varian M-6 diffusion pump is a standard,
they offer also TP version with 150 l/sec pump. So, they installed less
productive TP, perhaps to reduce the cost. I think, your impressions about
the price of SEIKO based on comparison the TPs with different productivity
(may be I am wrong).

As for Gary's remark about SEIKO controller, it's much smaller
now. Previous models has battery installed inside controller to support
active suspension during emergence shout downs. The modern models uses
internal TP generator to support themselves in that case. The controller
is comparable with my BALZERS controller in size. The wiring should be
different for sure and perhaps SEIKO's wiring is more complicated. From
another hand, SEIKO's TP do not required cooling, which is a nice feature I
think. Controller, unfortunately, does not have RS-232 interface. It has
a simple interface based on logistic like: contacts #1 and say #5
connected/disconnected by external relay. Using this things, you could
start/stop TP and control some simply features. It was useful to me: using
a few relays, electro-magnetic vacuum valves and signals from my vacuum
gauge, I was able to make fully automatic system, which operated by single
switch: AIR/VACUUM. Actually, I do have two more switches for safety
reasons: Main and switch to shout down the TP manually in between the cycle.

Thanks to all who mentioned where in EM-world SEIKO pumps has been used (I
am really happy for Hitachi and Topcon who choose this pump for their
microscopes).


I am not related to EDWARDS or SEIKO and have no any interest in that
companies except I am happy with their products and services.

Thanks.

Sergey.

At 01:43 PM 5/26/01 -0700, you wrote:
} The Seiko pumps are not made to withstand the constant cycling from
} atmosphere to high vacuum that the Balzers pumps will endure.
}
} ISI/ Topcon has valving assembly which isolates the turbo from this cycling.
} the Cambridge/ Leo & later Amray pump and vent the entire pumping system
} each time a sample is introduced.
}
}
}
} Earl
}
} ----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "Sergey Ryazantsev" {sryazant-at-ucla.edu}
} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Saturday, May 26, 2001 7:22 AM
} Subject: Re: Balzers Turbo Pumps
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } ISI/Topcon uses/used these pumps too. They are very nice and quite
} } reliable. I think the reason the masses don't use them is because they
} } are about four times more costly than the single mag bearing pumps
} } like the Balzers. If they got close to the cost of the Balzers, they
} } certainly would be strong contenders for replacement units. The down
} } side is the re-wiring it would take to incorporate the Seiko units.
} } Most of the controllers are much larger than those for the Balzers.
} } And the in and out signals may be different.
} }
} } gary g.
} }
} }
} } At 02:02 AM 5/26/2001, you wrote:
} }
} } } EDWARDS distributes SEIKO SEIKI magnetically levitated (active magnetic
} } } suspension of both ends) turbopumps. I am using STM-450 (450 l/sec) for
} 2
} } } years and really happy with this stuff. Extremely easy to operate
} } } (actually stop/start button). Don't need any cooling (even air flow).
} Any
} } } orientation. And supposed to be completely vibration-free, because the
} } } rotor have no contact with "body". In case of emergency stop it uses
} } } internal electricity generator to power controller and suspension.
} Active
} } } suspension protects from any disbalance in the system. Does not require
} } } any technical assistance. These pumps are pricely, but EDWARDS kindly
} } } offered to me very good discount. These pumps are work-horses in
} industry
} } } but rare in EM community. I have no idea why. Please, let me know if you
} } } need more details.
} } }
} } } No commercial interest, just happy user and owner.
} } }
} } } Sergey
} } }
} } } At 06:24 PM 5/25/01 -0500, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Listers,
} } } } It appears that Pfeiffer(Balzers) Vacuum Company will no longer be
} } } } supporting its older turbo pumps, like the TPH 170, 240, etc. No
} parts,
} } } } no repairs, nothing. I received notification from Pfeiffer that this
} } } } will be effective Jan. 1, 2002. Of course, they want you to buy their
} } } } "new" pumps. All of the independent turbo repair shops are scrambling
} } } } trying to find parts for the pumps they are repairing now, or have
} } } } commitments to repair in the future. Naturally, the cost of repairs to
} } } } these pumps has skyrocketed. The attraction of the use of Balzers
} turbo
} } } } pumps in electron microscopes was the fact that this pump was in effect
} } } } "self-balancing", emitting very low mechanical vibrations. Has anyone
} } } } found any suitable replacement for this pump by another
} } } } manufacturer? Suitable meaning not only good vibration specs but also
} } } } reasonable in cost, for purchase and or to repair.
} } } }
} } } }
} } } }
} } } } Gary M. Easton
} } } } Scanners Corporation
} } }
} } } ------------------------------------------------------
} } }
} } } Sergey Ryazantsev, Ph.D.
} } } Electron Microscopy
} } } Department of Biological Chemistry, School of Medicine
} } } University of California, Los Angeles
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } (310) 825-1144 (office)
} } } Pager: (310) 845-0248
} } } FAX: (310) 206-5272 (departmental)
} } } mailto:sryazant-at-ucla.edu
} } }
} }
} }
} }

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu



From daemon Sun May 27 04:57:57 2001



From: cc4less-at-kinori.com
Date: Sun, 27 May 2001 02:53:10 -0700
Subject: 24hr customer support 12556

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{HTML} {HEAD} {TITLE} Take Control Of Your Conference Calls {/TITLE}
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From daemon Sun May 27 18:09:42 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 27 May 2001 17:59:49 -0500
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For most work a server does a computer being retired from the desk top is
fast enough to do the job. It usually needs a bigger hard drive and more
memory. Unless there are a lot of users logged on at once or a lot of
large files being moved most servers don't work very hard. Build two on to
go and one for a spare. If your department is like mine you should be able
to come up with a few 300 MHz Pentiums and they make nice Linux servers.
And use the high end machine for a better use or use the money for a
better use.

I think my server is running a 133 Pentium.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} From: "Karl Garsha" {keg-at-csd.uwm.edu}

} One other concept that comes to mind, which may or may not pertain to
the
} individual situation we're addressing, is the issue of clustering
} capability. Put simply, "clustering" provides for the linking together
of
} several servers over a high-speed local area network for puposes of
ensuring
} availability of server services in the event of a hardware malfunction,
or
} to balance the load on a heavily used server between multiple machines,
or
} both. Software to accomplish this is readily available for Linux (ie.
} MOSIX: www.mosix.cs.huji.ac.il, and several others). I'm not entirely
sure
} if this can be accomplished under NT/2000, but I don't think so.
} Clustering capability may seem extravagant considering the
anticipated
} use the server will recieve initially, but a couple years from now one
might
} be dealing with greatly expanded server load, humongous files, an image
} database etc. In this case it may be attractive to hook together a
couple
} or few older machines rather than forcing the trusty primary server into
} early retirement.
} -Karl G.
}
} Karl Garsha
} www.uwm.edu/~keg
}




From daemon Sun May 27 18:09:42 2001



From: Palatsides, Manuela :      m.palatsides-at-pmci.unimelb.edu.au
Date: Mon, 28 May 2001 08:56:49 +1000
Subject: TEM: Ruthenium Red

Contents Retrieved from Microscopy Listserver Archives
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Hi
I looking for a method for the staining of cell culture cells with ruthenium
red for TEM. If have a method that works really well, I would appreciate a
copy.
Many thanks
Manuela Palatsides
Electron Microscopy
Peter MacCallum Cancer Institute
Locked Bag#1
A'Beckett Street
Melbourne 3000

Telephone: 03 96561244
Fax: 03 96561411
Email: m.palatsides-at-pmci.unimelb.edu.au






From daemon Sun May 27 19:15:47 2001



From: Karl Garsha :      keg-at-csd.uwm.edu
Date: Sun, 27 May 2001 19:15:35 -0500
Subject: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One other concept that comes to mind, which may or may not pertain to the
individual situation we're addressing, is the issue of clustering
capability. Put simply, "clustering" provides for the linking together of
several servers over a high-speed local area network for puposes of ensuring
availability of server services in the event of a hardware malfunction, or
to balance the load on a heavily used server between multiple machines, or
both. Software to accomplish this is readily available for Linux (ie.
MOSIX: www.mosix.cs.huji.ac.il, and several others). I'm not entirely sure
if this can be accomplished under NT/2000, but I don't think so.
Clustering capability may seem extravagant considering the anticipated
use the server will recieve initially, but a couple years from now one might
be dealing with greatly expanded server load, humongous files, an image
database etc. In this case it may be attractive to hook together a couple
or few older machines rather than forcing the trusty primary server into
early retirement.
-Karl G.

****************************************************************************
****************************************************************************
******
Karl Garsha
www.uwm.edu/~keg



From daemon Mon May 28 09:04:03 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Mon, 28 May 2001 08:56:12 -0500 (CDT)
Subject: B&W monitors

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have any suggestions for sources of good B&W
(TV-rate) monitors?

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html



From daemon Mon May 28 09:39:11 2001



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Mon, 28 May 2001 16:23:06 +0300 (EEST)
Subject: etching and polishind

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir or Madam;
Does anyone suggest suitable acids for etching and polishing of
Cu-Mn(25%)-Al(11%) alloys and Cu-Mn(25%)-Zn(11%),Cu-Mn(25%) and Mf and
Af temperature alloys?
Thanks for your interested.

**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************








From daemon Mon May 28 09:39:11 2001



From: megaprpf-at-juno.com ()
Date: Mon, 28 May 2001 09:36:55 -0500
Subject: Ask-A-Microscopist: TEM polymer in a oven?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(megaprpf-at-juno.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, May
28, 2001 at 03:31:58
---------------------------------------------------------------------------

Email: megaprpf-at-juno.com
Name: Robert Bauman

Organization: Amarillo College

Education: Undergraduate College

Location: City, State, Country

Question: How long does one leave TEM polymer in a oven to harden it
enough so that it can be sectioned on an ultramicrotome?

---------------------------------------------------------------------------


From daemon Mon May 28 13:46:04 2001



From: COURYHOUSE-at-aol.com
Date: Mon, 28 May 2001 14:39:50 EDT
Subject: Re: B&W monitors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Electronic Surplus houses tend to get these... I have even heard stories of
them being scrapped since the market is soft for them and some times dealers
get a large lot.... They will put a few in the store and send the rest out
for scrap.

thanks Ed Sharpe archivist for SMECC

{ { Subj: Re: B&W monitors
Date: 5/28/01 10:43:01 AM US Mountain Standard Time
From: {A HREF="mailto:COURYHOUSE"} COURYHOUSE {/A}
To: {A HREF="mailto:ldm-at-risc4.numis.nwu.edu"} ldm-at-risc4.numis.nwu.edu {/A}
To: {A HREF="mailto:microscopy-at-sparc5.microscopy.com"}
microscopy-at-sparc5.microscopy.com {/A}

Electronic Surplus houses tend to get these... I have even heard stories of
them being scrapped since the market is soft for them and some times dealers
get a large lot.... They will put a few in the store and send the rest out
for scrap.

thanks Ed Sharpe archivist for SMECC } }


From daemon Mon May 28 13:49:08 2001



From: Dra. Carmen Bracho :      cbracho-at-ivic.ve
Date: Mon, 28 May 2001 14:49:30 -0400
Subject: Cryo ultramicrotome-opinions

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I am considering purchase of a cryo ultramicrotome from Micro Star
Technologies. I would appreciate comments from anyone who has had
experience (good or bad) with this equipment and service.

Thanks in advance

Carmen Bracho
Centro de Microbiologia, IVIC
Phone/fax: + 58 212 504 17 59
e-mail: cbracho-at-ivic.ve



From daemon Mon May 28 14:12:11 2001



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 May 2001 15:07:04 -0400 (EDT)
Subject: Re: subsitiute for glut

Contents Retrieved from Microscopy Listserver Archives
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We routinely get inspected by JCAHO and CAP (College of American
Pathologists). We've never had a problem with the inspectors. All EM
labs, that I know of, including clinical ones, use glut for fixation.
Find out just what the problem was--labeling, handling, or the fact that it
was used at all.


Sara


On Fri, 25 May 2001 JHoffpa464-at-aol.com-at-sparc5.microscopy.com wrote:

} Date: Fri, 25 May 2001 18:25:35 -0500
} From: JHoffpa464-at-aol.com-at-sparc5.microscopy.com
} To: Microscopy-at-sparc5.microscopy.com
} Subject: subsitiute for glut
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hi all someone posed a question to me after being inspected by JCAHO the
} } inspectors were upset they were using glut. i had never heard of a good
} } subsitiute for glut so i thought i would throw it out to you guys. he
} didn't
} } say if it was a labeling problem or just the fact they had glut. i have
} never
} } heard of such a thing before.
} } john hoffpauir
} } electron microscopy lab
} } cooper hospital camden nj
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon May 28 15:11:46 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 28 May 2001 12:54:17 -0700
Subject: Micrographs needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been contacted by a retired biologist who runs a large photo service
for publishers who need images. He says he can use a wide variety of
subjects & he's particularly interested in the newer microscopies - "STEM,
atomic force, molecular probe, fluorescent and confocal, etc." Please
contact him directly if you have some good stuff to share:

} Photographs utilizing all types of microscopy are solicited for use in
} science textbooks at all levels, children's science books, trade books, field
} guides, aquarium and museum exhibits, TV programs (e.g., NOVA, Discovery
} Channel), and other educational publications. Credit and appropriate payment
} is given for each usage. Contact Dr. John D. Cunningham, VU Research/Visuals
} Unlimited, 46 The Flume, Amherst, NH 03031 for information. Ph: (603)
} 673-4653 Fax: (603) 673-4738 Email: john-at-visualsunlimited.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon May 28 15:29:22 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 28 May 2001 15:25:24 -0500
Subject: Re: B&W monitors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "L. D. Marks" {ldm-at-risc4.numis.nwu.edu}

} Does anyone have any suggestions for sources of good B&W
} (TV-rate) monitors?
}

Your video production people on campus should be able to give you sources
for them as well and may have surplus monitors on hand. If they can't help
the local TV stations should be able to help.

Look in the yellow pages under security systems if you want to buy new
ones locally.

If price is a problem get in touch with some local amateur radio operators
and ask about the fellows working with TV broadcasting. They should have
some or know where to get some for very reasonable prices. They will
probably still have the university inventory stickers on them from the
university auctions.

Yahoo shopping show a number of them as well
http://search.shopping.yahoo.com/search/all?P=all&is=1&p=%22B%26w+monitor%
22&cl=dmiyg


Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger





From daemon Mon May 28 16:40:14 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Monday, May 28, 2001 12:55 PM
Subject: B&W monitors

Contents Retrieved from Microscopy Listserver Archives
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Laurence,

Assuming that you are looking for the composite video monitor (video and
sync. signals mixed, as in common household TV set- all in single wire, and
75 Ohm input impedance, though security monitor usually has input switch for
high and low impedance): your most economical bet will be security hardware
vendors. You will find many in your local ph. book. Try also these:
www.supercircuits.com or (800)335-9777 ; and www.radioshack.com or
(800)291-6515. Both have catalogs online or available by mail. Use monitor
with vertical and horizontal hold controls for the best compatibility with
your signal source.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: L. D. Marks {ldm-at-risc4.numis.nwu.edu}
To: Microscopy List {microscopy-at-sparc5.microscopy.com}



From daemon Mon May 28 23:34:53 2001



From: M, Prabhakar (CORP, GEITC) :      M.Prabhakar-at-geind.ge.com
Date: Tue, 29 May 2001 09:56:26 +0530
Subject: Banding problem in BEI image

Contents Retrieved from Microscopy Listserver Archives
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Does any one know of the banding problem in the 2 Quadrant solid state Back
scatter electron image in JEOL 6335 FESEM?
We see vertical bands mixed with the BEI image. The service engineer says
that this is because of ground leakage current.
We measured the ground potential of 200 mv peak to peak w.r.t the chassis.
Although we try to believe that this is because of inefficient sheilding,
there could be other reasons too. Request you all to suggest what could be
wrong.

Thanks well in advance
Prabhakar M

GE India Technology Centre
________________________________________________
M.Prabhakar
PSM Group
John F. Welch Technology Centre
Export Promotion Industrial Park, Phase II
Hoodi Village, Whitefield Road
Bangalore-560 066, India
Tel.No. : 080-8412050-69 Extn. : 2574
Fax. : 080-8412111
E-mail: M.Prabhakar-at-geind.ge.com



From daemon Tue May 29 00:18:25 2001



From: Colin Reid :      creid-at-tcd.ie
Date: Tue, 29 May 2001 06:19:55 +0100
Subject: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

As part of the introduction of a quality system in our laboratory I have
been asked to write SOP's for the operation of the SEM and X-Ray analysis
system. I am not exactly sure what is required for this and wondered if
anyone else has been through this process. I would welcome advice as to
how much information is required on the actual operation of the equipment
and what other information is needed.

Regarding a previous request of mine about "Remote Access to SEM" I would
like to thank the people who responded. I had hoped to reply before now
but the process is ongoing. I hope to be able to report a successful
solution in the near future.

Many Thanks.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2.

Tel: 353-1-6081820
Fax: 353-1-6770438
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm



From daemon Tue May 29 03:11:57 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 25 May 2001 18:24:47 -0500
Subject: Balzers Turbo Pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I was affraid from that information, as I have a dozen from these types of
turbo pumps in service in our lab, so I have asked Pfeiffer in France what
about this thing. So today the answer is clear : in France they continue
to do maintenance on both type. For repear, only rotor and motor for 170
are not avaible, but for 240 all is avable, service and parts. They said
it seems to be an "US" problem. Probably, the reprentativ want to sell new
pumps... But its seems to be according to the general commercial politic
of this manufatcurer in the last years.

Three years ago, I hade a TPU 330 to repear (the dual rotor type, the
rotor was in marmalade), and they propose me an exchange with an new 240
for the price of repear. They could repear it, but it didn't pay.

As an other type of pump, I use the Varian V70 and V250.They are very
reliable, the controller gives much informations than can be used to drive
it carrefully. But as my use is for UHV surface analysis and plasma
coating, vibration is not a criterion, and I have some doubt about their
level for microscopy. And it is loudy... But I think that Leo use it on
the Gemini 1530/50.

We have also a Seiko Seiki magnetic bearing model, whitch sees often air
(vacuum evaporator for organic compounds). The pump is reliable, but not
the electronic. And you must send both back for repear. The after sale is
long (6 month one time), done by Edwards.

It's the same thing with a Pfeiffer TPU520-M ( full dynamic magnetic
bearings) on UHV STM. The pump itself is reliable, very good on vibration
aspect, the electronic less good. But with this type of pump, we have an
other limiation : the vessel is on an air sustended table, and if you
knock in the vessel, the pump will act as a gyroscope and make an
emergency stop. It doesn't like too much that kind of stop. It would be
the same with a SEM. OK, it is not something to do, to knock a STM or a
SEM in work !

I have now bought an new Pfeiffer (TMU 261), but it is unpacked today.
I'll say something about it in a few month. The controller is not
compatible with the former series (TPU/H 110,170,240,260,33,520), and vice
versa.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

---------- Forwarded message ----------


Listers,
It appears that Pfeiffer(Balzers) Vacuum Company will no longer
be supporting its older turbo pumps, like the TPH 170, 240, etc. No
parts, no repairs, nothing. I received notification from Pfeiffer
that this will be effective Jan. 1, 2002. Of course, they want you
to buy their "new" pumps. All of the independent turbo repair shops
are scrambling trying to find parts for the pumps they are repairing
now, or have commitments to repair in the future. Naturally, the
cost of repairs to these pumps has skyrocketed. The attraction of
the use of Balzers turbo pumps in electron microscopes was the fact
that this pump was in effect "self-balancing", emitting very low
mechanical vibrations. Has anyone found any suitable replacement for
this pump by another manufacturer? Suitable meaning not only good
vibration specs but also reasonable in cost, for purchase and or to
repair.



Gary M. Easton
Scanners Corporation



From daemon Tue May 29 04:19:33 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Tue, 29 May 2001 04:15:53 -0500
Subject: RE: Banding problem in BEI image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


BSE amplifiers typically run at high gains. That makes them susceptible to
many kinds of interference. Yes, it could be a grounding problem (one
would hope that the manufacturer's service rep could take care of that).
It could also be induced through the pre-amplifier and the cabling.
Coaxial cables only partially shield their signal wire. If there is
anything near the pre-amp or cabling that is capable of producing EMF
(including portions of the instrument itself), it will probably be picked
up by the system.

Try turning off or moving any ancillary equipment around the SEM. This
would include any external vacuum gauges, EDS systems and monitors and any
motors (chillers or mechanical pumps). Try re-routing the cabling to the
BSE detector and pre-amp. Once the source is identified, you can take
steps to minimize the problem.

One question does have to be asked, though. BSE imaging is a method to
distinguish between areas of differing atomic number averages. The closer
those atomic number differences, the more gain you will use to
differentiate them. If you are placing unrealistic expectations on your
system by expecting large image contrast from small differences in average
atomic numbers, you may well be pushing the capabilities of your system.
Can you provide more information regarding the materials you are seeking
to image?

On Monday, May 28, 2001 11:26 PM, M, Prabhakar (CORP, GEITC)
[SMTP:M.Prabhakar-at-geind.ge.com] wrote:
}
}
} Does any one know of the banding problem in the 2 Quadrant solid state
Back
} scatter electron image in JEOL 6335 FESEM?
} We see vertical bands mixed with the BEI image. The service engineer
says
} that this is because of ground leakage current.
} We measured the ground potential of 200 mv peak to peak w.r.t the
chassis.
} Although we try to believe that this is because of inefficient sheilding,
} there could be other reasons too. Request you all to suggest what could
be
} wrong.
}
} Thanks well in advance
} Prabhakar M
}
} GE India Technology Centre
} ________________________________________________
} M.Prabhakar
} PSM Group
} John F. Welch Technology Centre
} Export Promotion Industrial Park, Phase II
} Hoodi Village, Whitefield Road
} Bangalore-560 066, India
} Tel.No. : 080-8412050-69 Extn. : 2574
} Fax. : 080-8412111
} E-mail: M.Prabhakar-at-geind.ge.com
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Tue May 29 04:20:17 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Tue, 29 May 2001 04:18:17 -0500
Subject: RE: Micrographs needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe that everyone on this listserver has already received this request.

On Monday, May 28, 2001 2:54 PM, Caroline Schooley [SMTP:schooley-at-mcn.org] wrote:
}
}
} I've been contacted by a retired biologist who runs a large photo service
} for publishers who need images. He says he can use a wide variety of
} subjects & he's particularly interested in the newer microscopies - "STEM,
} atomic force, molecular probe, fluorescent and confocal, etc." Please
} contact him directly if you have some good stuff to share:
}
} } Photographs utilizing all types of microscopy are solicited for use in
} } science textbooks at all levels, children's science books, trade books, field
} } guides, aquarium and museum exhibits, TV programs (e.g., NOVA, Discovery
} } Channel), and other educational publications. Credit and appropriate payment
} } is given for each usage. Contact Dr. John D. Cunningham, VU Research/Visuals
} } Unlimited, 46 The Flume, Amherst, NH 03031 for information. Ph: (603)
} } 673-4653 Fax: (603) 673-4738 Email: john-at-visualsunlimited.
}
} Caroline
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Tue May 29 07:02:40 2001



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Tue, 29 May 2001 18:56:43 +0700
Subject: help for Jem4000EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers!

We have the problems with our JEM-4000EX: the tremblihg of the
electron beam spot.
Short history.
The filament in our microscope work more 800 hours.
High tension there was stable at 400kV. But the electron beam began to
tremble. The beam spot on the screen aperiodic tremble in one
direction with frequency from 1 to 20 Hz. Sometimes, fluctuations stop
and all there was OK. But with each daytime situation grew worse. In
addition increase the beam current : there was 123.9 (400Ë×), became
124.4. At 300kV there was 92.2, became 92.7.
We have to change the
filament. Here we have clean up of the Wehnelt and first anode as well
have changed the silicon grease on insulator. After change the
filament made the pumping of the microscope 2 days. Then we made all
operation according to instruction "High voltage generation after
filament exchange": annealing of the filament at îô=0kV etc. As the
HT at 400 kV there was unstable ( the fluctuations of vacuum meter
SIP-GUN) we use the N2 glow discharge conditioning at 150kV. Then once
again we made the filament annealing at HT=0kV. High tension became
stable at 400 kV, but the beam spot on the screen begins aperiodic
tremble at 370 kV and above (as before change the filament). The
training of HT for 3 days a situation did not perfect.
We have opened
the gun once again. On insulator and on Wehnelt have not the traces of
disruption. The checkup of the filament has shown that crystal LaB6
stand orderly, but fastening its to threads of incandescence (do not
know what this alloy) on the one hand flake away.
We have changed the
filament on new. And have done once again all procedures, described
above. The situation did not change, but even grew worse. The beam
current increase to 127mA at 400Ë×. The fluctuations of beam spot
begin at 350kV.
I have the following questions.
1. How to check with than is the
trembling of the electron spot : contamination in Gun or Column of the
microscope or instability of a electronic circuits? I think that the
high tension is stable since in diffraction mode the size of the
caustic is stable and not changed.
2. The new filaments are kept in
original packaging, but not in vacuum. Time of keeping more than 10
years. Can be the filaments degrade for this time?
3. The electron
spot trembling are fixed on the oscilloscope picture in check point CH
(HV tank) (In position OPERATE). In position TEST of the ACCEL VOLTAGE
switch the oscilloscope picture is stable (direct line). As it is
generated HT in position TEST?
Looking forward to hear some
recommendation which can help us.
In Russia we have not regularservice of JEOL Ltd.
Thank you very much in advance.
I am not currently part of the list, so please mail to me directly.
Best regards,
Anton Gutakovskii
mailto:gut-at-thermo.isp.nsc.ru



From daemon Tue May 29 08:24:50 2001



From: M, Prabhakar (CORP, GEITC) :      M.Prabhakar-at-geind.ge.com
Date: Tue, 29 May 2001 08:19:22 -0500
Subject: Banding problem in BEI image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does any one know of the banding problem in the 2 Quadrant solid state Back
scatter electron image in JEOL 6335 FESEM?
We see vertical bands mixed with the BEI image. The service engineer says
that this is because of ground leakage current.
We measured the ground potential of 200 mv peak to peak w.r.t the chassis.
Although we try to believe that this is because of inefficient sheilding,
there could be other reasons too. Request you all to suggest what could be
wrong.

Thanks well in advance
Prabhakar M

GE India Technology Centre
________________________________________________
M.Prabhakar
PSM Group
John F. Welch Technology Centre
Export Promotion Industrial Park, Phase II
Hoodi Village, Whitefield Road
Bangalore-560 066, India
Tel.No. : 080-8412050-69 Extn. : 2574
Fax. : 080-8412111
E-mail: M.Prabhakar-at-geind.ge.com


From daemon Tue May 29 09:35:22 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 29 May 2001 10:25:56 -0400
Subject: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had the pleasure (cough, cough) of writing SOP's (we call them SLP's in QS9000, ISO equivalent for the automotive industry, for system level procedures). Basically, what you need to do is write a set of procedures that you can live with. The amount of detail is really up to you. If you have experienced people that do not change very much, then they don't have to be very long. If you have a high turnover and people need to be trained frequently, then you can use the SLP as a training aid. They do come in handy when a person does some tasks infrequently such as cleaning wehnelts, changing filaments, or mixing chemicals.

What you will be required to do however is follow the procedures that you write down. If you don't, you can get written up for a non-conformance and that could be job-threatening. The more specific you are, the less "play" you have in using the instruments. Therefore, you should sprinkle in phrases such as "depending on operator experience," "suggested" and "recommended" liberally throughout the document. For example, the following is an excerpt from the section of the details to the procedures for our TEM SLP (it is in boldface and all caps to get the attention.:

Procedures: I: NOT ALL OF THE STEPS LISTED IN THIS PROCEDURE ARE REQUIRED TO BE FOLLOWED. OPERATOR EXPERIENCE AND TRAINING ARE USED TO JUDGE WHICH STEPS ARE APPLICABLE AND IN WHAT ORDER TO BEST APPLY THESE STEPS.

I suggest writing things down that are very specific with respect to service, calibration procedures, and things that you need to do to protect the microscope. These can be done in an outline form. Anything that you do routinely you can put down specifically. Other types of work, you should be more general in what you say so that you don't get pinned down on doing the work in a regimented way. You have to give yourself some flexibility to use the power of the microscope. What you should realize is that you are the expert, -not the examiner. All he/she is going to do is determine whether the paper trail on your work is there and if you follow your procedures. So, CYA!

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Colin Reid [mailto:creid-at-tcd.ie]
Sent: Tuesday, May 29, 2001 1:20 AM
To: MSA Listserver


Hi,

As part of the introduction of a quality system in our laboratory I have
been asked to write SOP's for the operation of the SEM and X-Ray analysis
system. I am not exactly sure what is required for this and wondered if
anyone else has been through this process. I would welcome advice as to
how much information is required on the actual operation of the equipment
and what other information is needed.

Regarding a previous request of mine about "Remote Access to SEM" I would
like to thank the people who responded. I had hoped to reply before now
but the process is ongoing. I hope to be able to report a successful
solution in the near future.

Many Thanks.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2.

Tel: 353-1-6081820
Fax: 353-1-6770438
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm



From daemon Tue May 29 09:39:41 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 29 May 2001 09:35:27 -0500
Subject: SEM/EDS: Analysis of cheese

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

We have a client who wants to look at how calcium is localized in processed
cheese samples. She thinks it may be in the fats and lipids, but that's
part of the research question. My question is: does anyone have any
knowledge of how to prepare cheese samples for EDS analysis in an SEM?

My inclination would be to do a gluteraldehyde fix and skip the heavy metal
post-fixes to avoid overwhelming the Ca signal with Os and UA signals, but
then this might leave a lot of lipid content unfixed and susceptible to
extraction during dehydration. We don't have an environmental scope,
unfortunately.

Any thoughts would be very welcome.

Thanks,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Tue May 29 10:47:44 2001



From: Katjaalex-at-aol.com
Date: Tue, 29 May 2001 11:41:38 EDT
Subject: Jeol 1200 EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, we have a problem with switching on the unit. If you turn the key, it
will start, but when you release the key, it stops immediately. The Forepump
does not start, we have enough air & water. All fuses check OK. Any ideas?
Thank you!
Peter Stolzenberg


From daemon Tue May 29 11:15:07 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Tue, 29 May 2001 09:09:57 -0700
Subject: Fuss over argon?

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Several years ago, during preparation of zirconium/zirconium oxide cross section
samples for TEM, we observed that the metal sometimes became completely
converted to Zr hydride during argon ion micromilling. The hydriding effect in
reactive metals such as Zr and Ti during ion milling had been recognized and
studied in some depth earlier as described by Carpenter et al. in the J. Micros.
Soc. of America, Vol. 1, No. 4 (1995) 175-184. Although their study identified
hydrocarbon contamination as the cause, the operating conditions in our case
pointed to water vapor in the argon. Ordinary grades of Ar including
"prepurified" grade actually have a rather high dewpoint. Among several
measures taken to eliminate the hydriding artifact, changing to ultrahigh purity
grade had the greatest, immediately evident effect. We also installed inline
molecular sieve/charcoal filters in the gas feed system ( most gas suppliers
sell these filters for removing specific contaminants) and made it standard
practice to use an LN2 cold trap in the ion mill. Cooling the sample also
would have been preferable, but the ion mill we were using did not allow this.

Larry Thomas

Larry Thomas
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto:Larry.Thomas-at-pnl.gov



} } I use cheap, industrial grade Argon which is run through
} } a Millipore molecular sieve. The sieve lasts for years.
} } The cylinder of Ar costs about $20 and also lasts for years.
} } The sieve assembly cost about $300. Virtually a one time
} } expense.
} }
} } I do the same for N2. I use industrial grade N2 ($20 per cylinder
} } rather than 9.8 N2 ($90 per cylinder). After two years, the N2
} } sieve is unchanged.
} }
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand





From daemon Tue May 29 11:33:01 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 29 May 2001 12:28:38 -0400
Subject: RE: Ruthenium Red

Contents Retrieved from Microscopy Listserver Archives
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Hi,
For Ruthenium Red,

First place I look is: Hayat, M.A.(1989), Principles and
Techniques of Electron Microscopy Biological Applications (3rd Ed.), CRC
Press, Boca Raton, FL, USA (ISBN: 0-8493-7111-2).pp285-289

Second is: Hayat, M.A.(1993), Stains and Cytochemical Methods,
Plenum Press, NY, NY, USA, (ISBN: 0-306-44294-9), pp305-318. (mention of
cultured cells), chemistry and (inter)actions.

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Palatsides, Manuela
} Sent: Sunday, May 27, 2001 6:56 PM
} To: microscopy list
} Subject: TEM: Ruthenium Red
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi
} I looking for a method for the staining of cell culture cells with
} ruthenium
} red for TEM. If have a method that works really well, I would appreciate
} a
} copy.
} Many thanks
} Manuela Palatsides
} Electron Microscopy
} Peter MacCallum Cancer Institute
} Locked Bag#1
} A'Beckett Street
} Melbourne 3000
}
} Telephone: 03 96561244
} Fax: 03 96561411
} Email: m.palatsides-at-pmci.unimelb.edu.au
}
}
}
}
}
}


From daemon Tue May 29 12:21:31 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 29 May 2001 13:15:40 -0400
Subject: RE: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
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The best SOP's you are likely to find are those prepared by SEM'ers involved
with Forensic analysis. This is a quick and dirty suggestion, however, and
is not intended to exclude any others who have confronted the issue in their
labs. Quality control in industry also likely requires rigorous SOP's, and
I am sure their are others. The Forensic folks talk to one another, and
they can be easily approached on the NET.

State of Virginia, USA, Forensic Science Lab might be a possibility.
http://www.dfs.state.va.us/

I searched GOOGLE for "Standard Operating Procedures SEM EDS and found among
the many the following.
http://www.plu.edu/~benhamsr/page20.html - probably what your leader would
like as a start.

Hope this helps,

Fred

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: Colin Reid
} Sent: Tuesday, May 29, 2001 1:19 AM
} To: MSA Listserver
} Subject: Writing SOP's for SEM/X-Ray Analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} As part of the introduction of a quality system in our laboratory I have
} been asked to write SOP's for the operation of the SEM and X-Ray analysis
} system. I am not exactly sure what is required for this and wondered if
} anyone else has been through this process. I would welcome advice as to
} how much information is required on the actual operation of the equipment
} and what other information is needed.
}
} Regarding a previous request of mine about "Remote Access to SEM" I would
} like to thank the people who responded. I had hoped to reply before now
} but the process is ongoing. I hope to be able to report a successful
} solution in the near future.
}
} Many Thanks.
}
} Best wishes,
}
} Colin
}
} Colin Reid,
} Electron Microscope Unit,
} Trinity College Dublin,
} Dublin 2.
}
} Tel: 353-1-6081820
} Fax: 353-1-6770438
} Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm
}
}
}


From daemon Tue May 29 12:28:58 2001



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Tue, 29 May 2001 13:25:09 -0400 (EDT)
Subject: Re: TEM: Ruthenium Red

Contents Retrieved from Microscopy Listserver Archives
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Manuela,

Here is a TEM prep that I use on confluent cell monolayers to demonstrate
the integrity of tight junctions.

30 minute fix in 2.5% glutaraldehyde in 0.1 M Sorensen's buffer, pH 7.4
and containing 0.1 % ruthenium red.

Two 3 minute rinses in 0.1 M Sorensen's buffer

Post fix for 15 minutes in 1 % osmium in Sorensen's buffer containing
1 % ruthenium red.

Two 3 minute rinses in buffer.

Quick rinse in ddH20 to remove salts.

en block stain for 15 minutes in aqueous 3 % uranyl acetate.

Dehydrate 3 minutes each in 50, 70, 90, and one change of 100 % EtOH.

Infiltrate with 3:1, 1:1, 1:3 EtOH:Epon for 20 minutes each.

Change to full strength epon for 1 hour.

Change to new full strength epon for 1 hour.

Polymerize

I hope this helps.

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Mon, 28 May 2001, Palatsides, Manuela wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi
} I looking for a method for the staining of cell culture cells with ruthenium
} red for TEM. If have a method that works really well, I would appreciate a
} copy.
} Many thanks
} Manuela Palatsides
} Electron Microscopy
} Peter MacCallum Cancer Institute
} Locked Bag#1
} A'Beckett Street
} Melbourne 3000
}
} Telephone: 03 96561244
} Fax: 03 96561411
} Email: m.palatsides-at-pmci.unimelb.edu.au
}
}
}
}
}



From daemon Tue May 29 12:41:00 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Tue, 29 May 2001 16:17:51 -0400
Subject: Re: Argon Purity

Contents Retrieved from Microscopy Listserver Archives
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Hi Peter,

Look at the magnetic sensor on the flywheel of your Roughing Pump. The
positioning of this unit is quit sensitive. Good luck.

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone 512/282-5507 FAX 512/280-0702

Sustaining Member - MICROSCOPY SOCIETY OF AMERICA

----- Original Message -----
} From: {"Katjaalex-at-aol.com"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 29, 2001 10:41 AM


Hi Mark,

Yes you are correct. In my mind if you are coating for SEM when you will
be running at magnifications of 15,000X upwards you will have almost
certainly have hidden or destroyed the specimen with a coating that looks
gold!

The heat that you report is understandable with long coating times I
believe it is fairly easy to understand.

1. We irradiate a target with argon (or air) gas molecules.
2. This heats up the surface as well as causing it to be sputtered, as
well as "polishing" the surface.
3. A polished hot surface will radiate heat - what sit opposite to
this target but your (now) hot specimens!

I have always argued that for an operator to fully understand a sputter
coating unit it takes about an hour of useful tests.

1. Work at a 5cm target to specimen distance.
2. Cover the base plate with a piece of photocopy paper (will contain
gas and possible moisture just like a specimen) place a small weight (an
SEM stub is ideal) on the paper to stop it blowing away.
3. Coat for several minutes at 20mA with a high voltage coater (1kV to
3kV with a variac type control) or for a minute with a low voltage coater
at 10mA ( has a deposition control not a kV control).
4. Look at the paper to see the coating distribution - almost
certainly there will be good and poor areas - note the position of the good
areas. Also note how round the stub the coating is not so good, this is
typical of areas around a specimen, they will affect the coating.
5. Using a similar piece of paper coat for multiples of 30 seconds on
fresh pieces of paper, for up to 3 minutes. Using the criteria set out
above for current and distance.
6 For typical SEM operations look for the coating that is the first
grey color that you obtain the colors will be - pink - light blue - darker
blue - grey - darker grey with gold tinges and eventually gold.
7. Place two small nuts (as in nuts and bolts) on copy paper on top of
each other, to create a hole about 1/4 inch high by 1/8th inch wide.
8. Run your coater and try every route at your disposal to get gold or
your preferred metal down the hole.

Once you have found out alI these answers I think you will know a great
deal more about your coater. You should now know -

a) What I call the target spots in your system, the positions for the
highest coating efficiency.
b) The coating criteria for a reasonable coat and minimum metal
structure.
c) The procedure for really difficult specimens with "tooth brush
type" structures. {Have you tried this one, its a bit of a surprise to find
that you cut off the gas and run at the best possible vacuum - think why?}

Never expect a sputter coating alone to overcome charging, if as an
operator you insist on using 15kV plus on difficult to coat specimens
expect trouble. To help think about tilting towards the Everhart-Thornley
detector to increase the BSE contribution to the image as BSE are not
troubled by the charge. With very complex specimens coat with the sample
tilted 45 degs in one direction then 45 in the other then use the "hole"
technique as described above to drive the metal into deeper areas. But as
I always say we are as electron microscopists "scientists" and scientists
should experiment :-)

Hope this helps, have fun!

Steve Chapman
Senior Consultant Protrain
For professional training wide wide in SEM, TEM and EDX
www.emcourses.com


From daemon Tue May 29 15:45:20 2001



From: Jill.Webb-at-rssl.com
Date: Tue, 29 May 2001 21:35:37 +0100
Subject: SEM/EDS: Analysis of cheese

Contents Retrieved from Microscopy Listserver Archives
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Dear Randy

When in the past I have looked at calcium/phosphorus distribution in cheese
it has been on frozen samples, using a Hexland (now Gatan) cryo-stage on
our SEM. Providing the fracture plane was OK, this worked generally well
and we achieved some quite interesting digital maps.

Regards

Jill

Jill Webb
Principal Scientist, Microscopy, RSSL

( Office : +44 (0)118 986 8541 ext 242
( Fax : +44 (0)118 986 8932
* jill.webb-at-rssl.com







"Tindall, Randy D." {TindallR-at-missouri.edu} on 29/05/2001 15:35:27

To: "'microscopy-at-sparc5.microscopy.com'"
{microscopy-at-sparc5.microscopy.com}
cc:



Hi Listers,

We have a client who wants to look at how calcium is localized in processed
cheese samples. She thinks it may be in the fats and lipids, but that's
part of the research question. My question is: does anyone have any
knowledge of how to prepare cheese samples for EDS analysis in an SEM?

My inclination would be to do a gluteraldehyde fix and skip the heavy metal
post-fixes to avoid overwhelming the Ca signal with Os and UA signals, but
then this might leave a lot of lipid content unfixed and susceptible to
extraction during dehydration. We don't have an environmental scope,
unfortunately.

Any thoughts would be very welcome.

Thanks,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/










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From daemon Tue May 29 16:59:41 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Tue, 29 May 2001 22:56:36 +0100
Subject: Re: SEM/EDS: Analysis of cheese

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think this is a job for Low-temperature SEM. Any attempt to do a
chemical fix risks extraction of soluble ions from the aqueous phase
of the cheese.
Chris

} Hi Listers,
}
} We have a client who wants to look at how calcium is localized in
processed
} cheese samples. She thinks it may be in the fats and lipids, but
that's
} part of the research question. My question is: does anyone have any
} knowledge of how to prepare cheese samples for EDS analysis in an
SEM?
}
} My inclination would be to do a gluteraldehyde fix and skip the
heavy metal
} post-fixes to avoid overwhelming the Ca signal with Os and UA
signals, but
} then this might leave a lot of lipid content unfixed and susceptible
to
} extraction during dehydration. We don't have an environmental
scope,
} unfortunately.
}
} Any thoughts would be very welcome.
}
} Thanks,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}



From daemon Tue May 29 17:29:12 2001



From: Tom Moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 29 May 2001 17:24:44 -0500
Subject: Oops

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To All,
Sorry about the post, It was meant to be private.
Tom
Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility
View expressed are my own.


From daemon Tue May 29 18:03:46 2001



From: Matthew M. Batchelor :      mmb-at-meyerinst.com
Date: Tue, 29 May 2001 17:59:59 -0500
Subject: 3Dreconstruction from 2D sections

Contents Retrieved from Microscopy Listserver Archives
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Kevin (and others),

Your question about 3D reconstruction based on 2D sections was forwarded to my
desk by an associate who is a member of your group.

There are many reconstruction packages on the market. One of the ones I am
aware of is the VoxBlast by VayTek (http://www.vaytek.com/VoxBlast.html).

Another I have seen is called SpyGlass but I do not have a URL
reference for that
one.

Best of luck.

Matt

Matthew M. Batchelor
Applications Engineer
------------------------------------
Meyer Instruments, Inc.
1304 Langham Creek, Suite 235
Houston, TX 77084
Video Microscopy Imaging Specialists
P - 281-579-0342, F - 281-579-1551
E-Mail = mmb-at-meyerinst.com
Home Page = http://www.meyerinst.com/


From daemon Tue May 29 22:20:09 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Wed, 30 May 2001 13:14:01 +1000
Subject: RE: Banding problem in BEI image

Contents Retrieved from Microscopy Listserver Archives
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If the banding is very regular, I'd be inclined to think its an electronic
problem. If its irregular, then I suspect a charging effect - is your specimen
well grounded? Try the BEI with just a blank mount.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, May 29, 2001 11:19 PM, M, Prabhakar (CORP, GEITC)
[SMTP:M.Prabhakar-at-geind.ge.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Does any one know of the banding problem in the 2 Quadrant solid state Back
} scatter electron image in JEOL 6335 FESEM?
} We see vertical bands mixed with the BEI image. The service engineer says
} that this is because of ground leakage current.
} We measured the ground potential of 200 mv peak to peak w.r.t the chassis.
} Although we try to believe that this is because of inefficient sheilding,
} there could be other reasons too. Request you all to suggest what could be
} wrong.
}
} Thanks well in advance
} Prabhakar M
}
} GE India Technology Centre
} ________________________________________________
} M.Prabhakar
} PSM Group
} John F. Welch Technology Centre
} Export Promotion Industrial Park, Phase II
} Hoodi Village, Whitefield Road
} Bangalore-560 066, India
} Tel.No. : 080-8412050-69 Extn. : 2574
} Fax. : 080-8412111
} E-mail: M.Prabhakar-at-geind.ge.com


From daemon Wed May 30 05:36:42 2001



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Wed, 30 May 2001 17:25:19 +0700
Subject: help for JEM4000EX

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Dear LISTERS-FRIENDS,
Thank your very much to everyone for your kind replies on my problems
with electron miocroscope JEM-4000EX.I hope that your recommendations
will be use to us.
Please find enclosed more details about our problems with JEM- 4000EX.
I check the stability îô by oscilloscope in óî at îô=280kV ( the
filament is off). The oscillogram is direct line, i.e. HT is stable.
Then I raise îô to 300kV. At first HT=300kV is stable. The beam
current is 93.5 mkA. In 3-5 min the beam current increase to 94.2 mkA
and a saw-tooth pulses appear on oscilloscope picture. The amplitude
of the pulse is ~0.2V, time length is about 5 mS and on-off time ratio
is 25-40 mS. When I reduce the îô to 280kV the saw-tooth pulses
present at oscilloscope picture already 20-30 min, but afterwards
disappear and îô becomes stable. When I raise HT to 300kV again then
the situation is repeated. The vacuum in GUN is 1.2x10-5Pa. The
fluctuation of the vacuum meter is half partition of the meter skale.
Previously vacuum was 6-7x10-6Pa. Have You some new ideas on this
cause? Thank you very much in advance.
Best regards
Anton
mailto:gut-at-thermo.isp.nsc.ru



From daemon Wed May 30 08:32:05 2001



From: Tim Lyden :      lyden.11-at-osu.edu
Date: Wed, 30 May 2001 10:31:50 +0100
Subject: Equipment for new lab.

Contents Retrieved from Microscopy Listserver Archives
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Hi all, I am writing to ask for advise and assistance in locating sources
of used equipment. I am starting a new position in the Fall at a smallish
undergrad campus in the U of Wisconsin system and am trying to setup a
basic microscopy center for both teaching and research. My funds are very
limited but I really need certain items, particularly a cryostat. Any help
would be greatly appreciated!!!! Please contact me directly at my current
address, lyden.11-at-osu.edu. Thanks in advance.


Tim Lyden, Ph.D.
Research Scientist
Heart and Lung Institute
Ohio State University



From daemon Wed May 30 08:55:06 2001



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Wed, 30 May 2001 14:51:15 +0100 (BST)
Subject: RE: Banding problem in BEI image

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I have experienced banding on BSE imaging in the past. It is almost always
caused by 50 (or 60) Hz interference from the electrical mains due to
inadequate earthing of cable shielding. Whether the problem is due to
mains frequency pickup can be seen by running at different scan rates -
the bands on the image should become narrower with slower scan rates.

David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-cam.ac.uk

}
} On Tuesday, May 29, 2001 11:19 PM, M, Prabhakar (CORP, GEITC)
} [SMTP:M.Prabhakar-at-geind.ge.com] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Does any one know of the banding problem in the 2 Quadrant solid state Back
} } scatter electron image in JEOL 6335 FESEM?
} } We see vertical bands mixed with the BEI image. The service engineer says
} } that this is because of ground leakage current.
} } We measured the ground potential of 200 mv peak to peak w.r.t the chassis.
} } Although we try to believe that this is because of inefficient sheilding,
} } there could be other reasons too. Request you all to suggest what could be
} } wrong.
} }
} } Thanks well in advance
} } Prabhakar M
} }
} } GE India Technology Centre
} } ________________________________________________
} } M.Prabhakar
} } PSM Group
} } John F. Welch Technology Centre
} } Export Promotion Industrial Park, Phase II
} } Hoodi Village, Whitefield Road
} } Bangalore-560 066, India
} } Tel.No. : 080-8412050-69 Extn. : 2574
} } Fax. : 080-8412111
} } E-mail: M.Prabhakar-at-geind.ge.com
}



From daemon Wed May 30 11:47:10 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 30 May 2001 09:39:05 -0700 (PDT)
Subject: Re: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colin:
In line with Scott's comments, (having had to do this same thing when moving
from academia to industry), the basic thing is to use the KISS principle
(i.e. keep it simple s----). We have used the approach to refer to the
equipment manual(s) as much as possible for actual instrument functions.
The major things expected in our SOPs (for the FDA) are: equipment log
(user, date, sample IDs, etc); equipment maintenance procedures (routine,
emergency, repair, etc, including chain of command, notification--ie out of
service posting--, signature of service personnel, and so on). Calibration
(standards, maintenance, frequency, ...) is also another biggie. Above all,
make it something you can live with, something that is not too detailed and,
most importantly, useful.

Roger
On Tue, 29 May 2001 06:19:55 +0100, Colin Reid wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hi,
|
| As part of the introduction of a quality system in our laboratory I have
| been asked to write SOP's for the operation of the SEM and X-Ray analysis
| system. I am not exactly sure what is required for this and wondered if
| anyone else has been through this process. I would welcome advice as to
| how much information is required on the actual operation of the equipment
| and what other information is needed.
|
| Regarding a previous request of mine about "Remote Access to SEM" I would
| like to thank the people who responded. I had hoped to reply before now
| but the process is ongoing. I hope to be able to report a successful
| solution in the near future.
|
| Many Thanks.
|
| Best wishes,
|
| Colin
|
| Colin Reid,
| Electron Microscope Unit,
| Trinity College Dublin,
| Dublin 2.
|
| Tel: 353-1-6081820
| Fax: 353-1-6770438
| Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed May 30 11:48:24 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 30 May 2001 09:44:23 -0700 (PDT)
Subject: Re: TEM: Ruthenium Red

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Manuela:
Good responses from Fred and Dorothy. The Hayat references are certainly
more accessible than the original papers by John Luft. The work by Luft,
however, demonstrated the need for a very good source of ruthenium red
(available from most EM supply houses nowadays). While not a terribly fussy
procedure, use of a poorly defined RR will give variable results. I wish
you the best.
Roger
On Mon, 28 May 2001 08:56:49 +1000, Palatsides, Manuela wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
|
| Hi
| I looking for a method for the staining of cell culture cells with
ruthenium
| red for TEM. If have a method that works really well, I would appreciate
a
| copy.
| Many thanks
| Manuela Palatsides
| Electron Microscopy
| Peter MacCallum Cancer Institute
| Locked Bag#1
| A'Beckett Street
| Melbourne 3000
|
| Telephone: 03 96561244
| Fax: 03 96561411
| Email: m.palatsides-at-pmci.unimelb.edu.au
|
|
|
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed May 30 11:50:08 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 30 May 2001 09:46:08 -0700 (PDT)
Subject: Re: Ask-A-Microscopist: TEM polymer in a oven?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I sent a response directly to the original sender of this message, but
received a "not a subscriber" error message in return. If I get a viable
address, I'll resend.
Roger
On Mon, 28 May 2001 09:36:55 -0500, megaprpf-at-juno.com wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Below is the result of your feedback form. It was submitted by
| (megaprpf-at-juno.com) from
| http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, May
| 28, 2001 at 03:31:58
|
---------------------------------------------------------------------------
|
| Email: megaprpf-at-juno.com
| Name: Robert Bauman
|
| Organization: Amarillo College
|
| Education: Undergraduate College
|
| Location: City, State, Country
|
| Question: How long does one leave TEM polymer in a oven to harden it
| enough so that it can be sectioned on an ultramicrotome?
|
|
---------------------------------------------------------------------------
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed May 30 16:02:12 2001



From: Nicole M. DeLance :      ndelance-at-zoo.uvm.edu
Date: Wed, 30 May 2001 16:55:21 -0400 (EDT)
Subject: Tygon tubing and SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I work in the Central Imaging Facility at the University of Vermont. WE have
had a request to look for bacteria on Tygon tubing using the SEM. THe request
came from a dentist. I was wondering if you could lend some insight as to how
we might go about doing this or if you know of someone that might be able to
help.
Thank you
Nicole


From daemon Wed May 30 17:02:49 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 31 May 2001 10:04:12 GMT+1200
Subject: Re: Banding problem in BEI image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





The origin can be magnetic, also, I had a similar problem with my
JEOL 840, which uses an external BSE preamp that sits on the table
beside the column. Maybe your model does, too.

I have also an optical microscope, whose lamp was being powered
by a small transformer, with mutiple taps on the secondary, which was
within 6" or so from the BSE preamp.

I had discounted this as the cause, because the transformer was
always switched to "off" whenever a BSE image was being collected,
so, I thought, no magnetic field.

However, relocating the transformer eliminated the banding. I guess
there was sufficient magnetic field radiated from the transformer
even when there was no current being drawn from the secondary.

So check that there are no AC-current-carrying leads nearby.

cheers

rtch


}
} Does any one know of the banding problem in the 2 Quadrant solid
} state Back scatter electron image in JEOL 6335 FESEM? We see
} vertical bands mixed with the BEI image. The service engineer
} says that this is because of ground leakage current. We measured the
} ground potential of 200 mv peak to peak w.r.t the chassis. Although
} we try to believe that this is because of inefficient sheilding,
} there could be other reasons too. Request you all to suggest what
} could be wrong.
}


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed May 30 17:37:03 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 30 May 2001 15:33:34 -0700
Subject: Re: SEM/EDS: Analysis of cheese

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy:

I have a paper I could send you that may be of interest. It is titled "High
Resolution Scanning Electron Microscopy of Milk Products: A New Sample
Preparation Procedure" by McManus, McMahon and Oberg. Food Structure, Volume
12 (1993) pp 475-482. The "new" part may not be so relevant as the paper is
from 1993.

Some of the materials they worked with were mozzarella, swiss and cream
cheeses. This really isn't my area of expertise, but I have the paper here
because they used our Ion Beam Sputtering system as part of the process. If
you would like a copy, I will be pleased to send it over to you.

Best regards-

David

"Tindall, Randy D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers,
}
} We have a client who wants to look at how calcium is localized in processed
} cheese samples. She thinks it may be in the fats and lipids, but that's
} part of the research question. My question is: does anyone have any
} knowledge of how to prepare cheese samples for EDS analysis in an SEM?
}
} My inclination would be to do a gluteraldehyde fix and skip the heavy metal
} post-fixes to avoid overwhelming the Ca signal with Os and UA signals, but
} then this might leave a lot of lipid content unfixed and susceptible to
} extraction during dehydration. We don't have an environmental scope,
} unfortunately.
}
} Any thoughts would be very welcome.
}
} Thanks,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Wed May 30 17:45:54 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 30 May 2001 15:41:34 -0700 (PDT)
Subject: Re: EM Lab Server OS: Linux or Windows 2000?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Retailed boxed isn't free but the same product minus the box is
free.

You can download and install linux over the net using ftp, or get a cd
with the full distribution of your flavour of linux for a low price
(~2-3$) at:

http://cart.cheapbytes.com/cgi-bin/cart
http://www.edmunds-enterprises.com/


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Sat, 26 May 2001, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Retail boxed Linux (Redhat, for example) is not free. It is about
} the same price as Win2K.
}
} The main problem is as you point out--application programs. While
} many are available for Wintel, there are few for Linux. Being an
} open system, Linux depends on having source code so one can
} compile the app for the specific Linux platform. This is unlikely to
} happen for industrial strength apps. They do not supply source code.
} And most probably, they do not support Linux.
}
} Nevertheless, Linux with X-windows on a P-III/733 or 933 is really awesome!
}
} gary g.
}
}
} At 08:33 PM 5/21/2001, you wrote:
}
} } I would agree that familiarity with the operating environment is important
} } for timely performance of server administration tasks-documentation and
} } advocacy groups exist for both platforms, however. It might be worth while
} } to become familiar with a UNIX like operating system (such as Linux) if one
} } has the luxury of time (I'll explain momentarily). Cost may be an
} } issue-Linux is free. Both OSes run on the x86 architecture.
} } It seems that your choice(s) of application software should be a
} } heavywieght factor in your decision. At the risk of over-simplification
} } I'll just say that most proprietary "out of the box" solutions are developed
} } for Windows/NT. There is a huge installed base of Wintel machines, and
} } there are powerful software development tools for this platform which allow
} } software developers to develop large complicated programs very rapidly.
} } Linux has the advantage that many programs written to run under the
} } various flavors of UNIX will compile and run. Many cutting edge scientific
} } applications, including image processing utilities, are developed by
} } researchers who are experienced coders and prefer to work under the UNIX
} } platform. The reason for this is partially historical-in the past only large
} } UNIX mainframe servers had the horsepower for image manipulation. More
} } often than not these software tools are available free of charge to fellow
} } researchers and are provided as open-source (one can view and modify the
} } original code to suit specialized situations).
} } So it really boils down to an ease of use vs. scalability/extensibility
} } issue with cost, and perhaps minor hardware decisions (sometimes peripherals
} } are difficult to configure under Linux ie. video cards, scanners etc...).
} } In the long run, however, time spent now learning to work with Linux may pay
} } off: the heart of the OS (the kernel) evolves continually in response to
} } the needs of users, and the kernel can be upgraded without disrupting other
} } parameters of the OS. Also, Linux runs on most (if not by now all)commonly
} } encountered chip architectures so one may have several different kinds of
} } machine (PC, Mac, SUN) all running the same operating system.
} }
} } Karl Garsha
} } www.uwm.edu/~keg
} } ----- Original Message -----
} } } From: "Chao-Ying Ni" {cni-at-udel.edu}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, May 18, 2001 9:36 AM
} } Subject: EM Lab Server OS: Linux or Windows 2000?
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear colleagues,
} } }
} } } I'm going to build an EM lab server to run web and other applications,
} } } to share files, especially to have an interactive wed scheduling software
} } } to schedule and monitor usage of our 2 TEMs and 2 SEMs. Right now I have
} } } narrow down the platform to Linux and Windows 2000. Could you please give
} } } me some inputs concerning the pros and cons of these two operating
} } } systems? Any comments and suggestions on the hardware selection are also
} } } more than welcome. Thanks in advance!
} } }
} } } Chaoying Ni
} } } Electron Microscopy Lab
} } } Dept. of Materials sci. and Eng.
} } } University of Delaware
} } } Newark, DE 19716
} } } cni-at-udel.edu
} } }
} } }
} } }
} } }
}
}



From daemon Wed May 30 17:56:41 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 30 May 2001 18:51:27 -0400
Subject: Re: SEM/EDS: Analysis of cheese

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

The best way would probably be with the use of a cryo stage on your SEM.
The process will preserve your samples in their most natural state. In
fact, Polaron uses a photo micrograph of cheese in some of its cryo
marketing materials.

We are the U.S. distributors for The Polaron Range, including their cryo
stage. Cryo systems are also available from other manufacturers such as
Gatan (formerly the Oxford System), Bal-Tec and Emmitech, to name a few.

I hope this helps.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"




-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, May 29, 2001 5:57 PM
To: Tindall, Randy D.
Cc: microscopy-at-sparc5.microscopy.com


I think this is a job for Low-temperature SEM. Any attempt to do a
chemical fix risks extraction of soluble ions from the aqueous phase
of the cheese.
Chris

} Hi Listers,
}
} We have a client who wants to look at how calcium is localized in
processed
} cheese samples. She thinks it may be in the fats and lipids, but
that's
} part of the research question. My question is: does anyone have any
} knowledge of how to prepare cheese samples for EDS analysis in an
SEM?
}
} My inclination would be to do a gluteraldehyde fix and skip the
heavy metal
} post-fixes to avoid overwhelming the Ca signal with Os and UA
signals, but
} then this might leave a lot of lipid content unfixed and susceptible
to
} extraction during dehydration. We don't have an environmental
scope,
} unfortunately.
}
} Any thoughts would be very welcome.
}
} Thanks,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}





From daemon Wed May 30 20:51:27 2001



From: joe.p.neilly-at-abbott.com
Date: Wed, 30 May 2001 20:46:47 -0500
Subject: Re: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I would agree with Scott's reply, make sure that you can live with the SOP
that you write. We are in the process of writing an operation SOP for our
SEM. We started with the operation manual and had an experienced user write
our SOP. We then had everyone in our department review the SOP. Some of the
non-users had some good suggestions for improvement. Finally, we had a new
user who was learning the SEM use the SOP and make suggestions. This sounds
like a lot of work, but after all that input we think we have a document that
we can work with and that will help us train future users. We did include
some statements that allowed for use of scientific judgement, but kept these
to a minimum.

Good Luck,

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com




"Colin Reid"
{creid-at-tcd.ie To: "MSA Listserver"
{Microscopy-at-sparc5.microscopy.com}
} cc:
Subject: Writing SOP's
for SEM/X-Ray Analysis
05/29/01
12:19 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

As part of the introduction of a quality system in our laboratory I have
been asked to write SOP's for the operation of the SEM and X-Ray analysis
system. I am not exactly sure what is required for this and wondered if
anyone else has been through this process. I would welcome advice as to
how much information is required on the actual operation of the equipment
and what other information is needed.

Regarding a previous request of mine about "Remote Access to SEM" I would
like to thank the people who responded. I had hoped to reply before now
but the process is ongoing. I hope to be able to report a successful
solution in the near future.

Many Thanks.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2.

Tel: 353-1-6081820
Fax: 353-1-6770438
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm


From daemon Wed May 30 21:28:13 2001



From: FABBRI :      fabbri-at-cigssrv1.unimo.it
Date: Thu, 31 May 2001 09:55:16 +0200
Subject: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Call Belair at 1-800-783-9424 they sell refurbished cryostats.

Diane
Miller Consultant Service
503-627-0130

----- Original Message -----
} From: "Tim Lyden" {lyden.11-at-osu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 30, 2001 2:31 AM



We are using a quite new FEI XL-30 LaB6 SEM.
It's a long time the we are trying to understand the
normal working conditions of the vacuum system. Looking
at what is written in the operating manual, our vacuum system is not able
to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
To do this we need to bakeout the gun frequently ( weekly )and are able
to work in that safety ( { 2x10-7 )condition only for few hours.
FEI says that the info on the manual are too restrictive (!!) and that
we can work safetly with vacuum levels {6 x10-7 baking out every two weeks.

Any thoughts would be very welcome.
Thank you very much in advance.

Best regards
P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------





From daemon Thu May 31 03:21:19 2001



From: Dujin Wang :      wangd-at-mpip-mainz.mpg.de
Date: Thu, 31 May 2001 09:54:09 +0200
Subject: TEM need help on embedding and cutting of ZnO crsytals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,
I am trying to do ultrathin sectioning work of ZnO crystals, which size
is about 2-3microns(L) and 1-2microns(W). I am really a freshman
on TEM, so I hope to get help on this work. Any suggestions for the
choosing of embedding media and following procedures will be greatly
appreciated.
Thank you in advance.

Dujin Wang
Max-Planck-Institute for Polymer Research
Ackermannweg 10, Mainz D-55128
Germany

Tel: 0049-6131-379226
Fax: 0049-6131-379100
Email: wangd-at-mpip-mainz.mpg.de



From daemon Thu May 31 04:57:04 2001



From: yimin yao :      yimin-at-fy.chalmers.se
Date: Thu, 31 May 2001 11:51:25 +0200
Subject: Graghic program for Bollmman's model

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am working in small particle contacting with TEM and I am interested in
interpreting the contacting boundary structure using Bollmman's geometric
model.

Can you offer me any information about the computerised way or software
which can graphically show the CSL misroientation solution of grain
boundary dislocation structures for cubic structure by methods based on that
of Bollmann.

Thank you,


Yiming
---------------------------------

Yiming Yao

Microscopy and Microanalysis
Experimental Physics
Chalmers University of Technology
SE-41296, Göteborg
Sweden

Tel: +46 31 7723633
Fax: +46 31 7723224
email: yimin-at-fy.chalmers.se

-----------------------------------




From daemon Thu May 31 06:56:35 2001



From: Keith Collins :      collins-at-alrc.doe.gov
Date: Thu, 31 May 2001 07:17:31 PST
Subject: Re: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Fabbri -

I take it from your message that the manual for the vacuum system is
independent of the manual for the XL-30. I think I would tend to believe
FEI. We operate our LaB6 routinely at 1 x 10-6.6 to 1 x 10-6.8 mbar. Our
slightly poorer vacuum probably does result in somewhat shortened LaB6 life,
but it's the best vacuum our aging ion pump can manage, and we rarely have
"down" time because of vacuum problems. In a perfect vacuum, of course, the
LaB6 filament would last a very, very long time, (theoretically); and the
poorer the vacuum, the shorter the filament life. But I think in this case
FEI is right - your vacuum system manual may be "too restrictive" for
real-world operation. I would consider 6 x 10-7 an admirable vacuum, at
least in our instrument. And, of course, FEI will be happy to sell you a new
LaB6 when you need one ;-).
If your new XL-30 is able to do its own "bake-out" routinely, then by all
means use this function as often as you feel is necessary. And do keep an
eye on the state of the vacuum - you may notice changes for the better or
worse as time goes on.
Good luck.

F.C. Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



----- Original Message -----
} From: "FABBRI" {fabbri-at-cigssrv1.unimo.it}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, May 31, 2001 4:55 AM


At the US Department of Energy Albany Research Center we
review our SOPs annually so that changes and suggestions can be
documented and included. I tell people that if they see needed
changes and suggestions for the SOPS let me know so these can
be incorporated. Everyone who operates our SEMs must read and
sign the SOP for that equipment.

It is my opinion that an SOP does not replace the manufacture's
operator and service manuals, software online help and operators
training. But I also realize that some management want very
detailed SOPS so I am thankful for scanners and word processors.

Keith Collins


From daemon Thu May 31 10:56:03 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Thu, 31 May 2001 11:53:12 -0400
Subject: RE: Equipment for new lab.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tim Lyden wrote:

} Hi all, I am writing to ask for advise and assistance in
} locating sources of used equipment...

} My funds are very limited...


Tim,

I am very much in the same boat. I am setting up an SEM lab out of my own
pocket - no funding from a university, corporation, or government. I have
had good success by constantly checking the auction sites www.labx.com and
www.ebay.com. Also, Duniway Stockroom of Mountain View, CA (www.duniway.com
800-446-8811) stocks a lot of supplies that you may need at very reasonable
prices.

The most important thing about the auction sites is patience. It takes a
while for some items to come up, but over the past six months, I have seen
two sputter coating units, lots of vacuum pumps, a critical point dryer,
lots of ultrasonic cleaners, and zillions of light microscopes. Plenty of
other stuff, too. I'm pretty sure that I've seen a cryostat, but since I
wasn't looking for one, I didn't pay any attention to it.

If you are patient and persistent, my experience indicates that you can buy
things at about 10 to 20 per cent of the original price. I am currently
sitting at my desk looking at a Polaron critical point dryer that just
arrived this morning. I bought it for $100. I am awaiting the arrival of a
Polaron sputter coater that I bought for $465. I bought a Varian ionization
gauge controller for $165 and the gauge tube (also Varian) for $35. I bought
the cable to connect the two from Duniway Stockroom for $89. Varian wanted
$190. The electron microscope itself came from none other than your new
employer, the University of Wisconsin, at no cost other than truck rental
and fuel.

The usual auction site disclaimers apply - Make sure that you know exactly
what you are bidding on - get model numbers and check the specs. Find out
whether or not the unit is known good, might be good, is good for parts
only, etc. Be willing to let go of an item if the bidding gets too high -
another item of the same type will eventually appear. Don't let you emotions
get the better of you. Set your budget limit and stick to it. Read the
auction rules. eBay and LabX operate differently. On eBay, they use proxy
bidding so that you don't have to watch the bidding so closely, but someone
can steal an item from you at the last second. LabX works more like a real
auction where you have to monitor the bidding closer, but if someone dumps
in a bid at the last second, the auction is extended for a period of two
minutes to allow you time to respond.

Good luck. The search can be fun, if a little frustrating at times. I wish
you well in your new position.

Bruce Girrell



From daemon Thu May 31 11:58:16 2001



From: Gregory W. Lum :      glum-at-sfsu.edu
Date: Thu, 31 May 2001 09:55:16 +0200
Subject: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
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Since the SEM is new, it should be still on warranty. However, get FEI
representative's advice in writing on company letterhead to protect
yourself. If you think the XL30 is not performing to specifications,
call them in to find out why it isn't.

Greg Lum
Lab Manager
EM Facility
San Francisco State University





---------- Forwarded message ----------



We are using a quite new FEI XL-30 LaB6 SEM.
It's a long time the we are trying to understand the
normal working conditions of the vacuum system. Looking
at what is written in the operating manual, our vacuum system is not able
to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
To do this we need to bakeout the gun frequently ( weekly )and are able
to work in that safety ( { 2x10-7 )condition only for few hours.
FEI says that the info on the manual are too restrictive (!!) and that
we can work safetly with vacuum levels {6 x10-7 baking out every two weeks.

Any thoughts would be very welcome.
Thank you very much in advance.

Best regards
P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------







From daemon Thu May 31 13:09:12 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 31 May 2001 14:06:16 -0400
Subject: RE: Used equip source

Contents Retrieved from Microscopy Listserver Archives
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In my files I find the following companies that deal in used and
rebuilt equipment:

Capovani Brothers Inc.
http://www.capovani.com
Tel: 518-346-8347

E. McGrath inc.
Tel 508-744-3546

Good luck,
WCB
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Thu May 31 14:49:57 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 31 May 2001 15:43:59 -0400
Subject: RE: cadmium stain

Contents Retrieved from Microscopy Listserver Archives
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Diana,

As usual I let my library do the walking.

Look in Thompson, S.W.(1966), "Selected [1600+ pages!!!!] Histochemical and
Histopathological Methods", Thomas Pub., Springfield, IL, USA

Chap 9. "Microscopic Histochemical Methods for the Demonstration of
Minerals"
p.p.. 587-589 Sodium rhodizonate (CAS 523-21-7).
Primary method for Lead, but Cd is listed a minor contributor under certain
conditions. While the Lead complex is violet and very strong, Cd complex is
'brown-red' and not as strong a Rx. Al Rx's are pH dependent. This is a
classic "in-your-hands" cytochemical method, but it is for LM. Just when
you think all is lost in the past, a little light appears, as follows.

ALSO, Cd is bound by oxime (also 8-hydroxyquinoline[CAS: 148-24-3]). The
latter is used to chelate Cd. See, Yoshizuka, M, McCarthy, K.J. Kaye, G.I.,
and Fujimoto, S.(1990), Cadmium toxicity to the cornea of pregnant rats:
Electron Microscopy and X-ray microanalysis, Anat. Rec., 227: 138.(from
Hayat,M.A.(1993), STAINS AND CYTOCHEMICAL METHODS, Plenum, N.Y., NY,
pp.375-76). The reference in Hayat is a bit sketchy, but the resolution for
you is about right. Fortunately, the primary record should be readily
available.

BUT, seems to me if there is sufficient Cd for there to be a
question, a good thin section (not from paraffin) would show it using STEM
or at lower res (you CAN use a paraffin section!) using SEM w/EDS or better
res w/WDS. Some college/university/company should have one of these and be
willing to help. With the right combination of a heavy metal 'stain' (lead
or uranium as long as it doesn't exchange with the Cd!), you should be able
to get a good tissue map with backscatter and then a great overlay of that
with the X-Ray map of the Cd distribution. Without the reference, I can't
back this memory up, but it seems to me that if you have an animal with
suspected Cd poisoning, one of the best places to look is the testis in
which it concentrates (I think!). Sort of like looking for Iodine in the
thyroid.

PubMed Search:

27. Kawahara A, Yoshizuka M, Hirano T, Ohsato K, Fujimoto S.
Related Articles Cadmium toxicity in perinatal rat hepatocytes: electron microscopy, X-ray
microanalysis, and morphometric analysis. Exp Mol Pathol. 1990 Oct;53(2):180-90. PMID: 2261947 [PubMed - indexed for MEDLINE] 28: Yoshizuka M, McCarthy KJ, Kaye GI, Fujimoto S. Related Articles Cadmium toxicity to the cornea of pregnant rats: electron microscopy and
x-ray microanalysis. Anat Rec. 1990 May;227(1):138-43. PMID: 2368924 [PubMed - indexed for MEDLINE]

Since you work at *.gov, this should not be an insurmountable
problem.

Good Luck and hope this helps,


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.

} ----------
} From: Diana M Papoulias
} Sent: Wednesday, May 23, 2001 7:48 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: cadmium stain
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am seeking assistance in finding a method to stain cadmium in paraffin
} embedd tissue. If such a method exists, I would very much appreciate your
} help.
}
} Thank you in advance,
}
} Diana Papoulias
} Fisheries Biologist, Research
} US Geological Survey
} Columbia Environmental Research Center
} 4200 New Haven Rd.
} Columbia, Missouri 65201
}
} voice mail: 573-876-1902
} e-mail: Diana_Papoulias-at-usgs.gov
} fax: 573-876-1896
}
}
}


From daemon Thu May 31 15:03:47 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 31 May 2001 12:59:58 -0700
Subject: LM- Video camera/microscope coupler

Contents Retrieved from Microscopy Listserver Archives
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Hi:

A colleague wishes to get guidance and information about coupling his video
camera to his compound microscope.

He has a Sony DXC-970MD video camera. The camera has an ENG 1/2" bayonet
lens mount. He wants to use it on a Zeiss Axiophot.

I am used to simple C-mount adapters, the bayonet mount is the ringer for me.

According to his research Sony offers an adapter, HRT045ENG12, that they
say is used to mount this camera to a microscope. His question is 'Does the
Sony adapter substitute for those offered by Zeiss for this purpose, or is
the Sony adapter used to convert the bayonet mount to something compatable
with the adapters offered by Zeiss?'

Finally, back in the old days, I remember learning that one could use a
simple T-mount to attach a 35 mm camera to a microscope, but that it was
not as good as having an eyepiece camera because it lacked the ocular lens.
Is the same true of mounting a video camera? I have always just used
simple, lenless C-mounts and it has seemed fine. If we are looking for
ultimate image quality, should we be using a more sophisticated adapter?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu May 31 15:33:30 2001



From: Danowski, Kristine (KL) :      KLDanowski-at-dow.com
Date: Thu, 31 May 2001 16:29:13 -0400
Subject: RE: Equipment for new lab.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tim Lyden wrote:

} Hi all, I am writing to ask for advise and assistance in
} locating sources of used equipment...

} My funds are very limited...


Hi Tim,

I have started new molecular biology and tissue culture labs. Several
vendors (e.g., Fisher Scientific) provide discounts for new lab startups.
For example, I called Fisher and they sent me some forms to complete. That
was it. Whenever I placed an order, I told the operator about my discount.
The discounts vary with each vendor, and they can save you a substantial
sum. If you must purchase new equipment and supplies, you might want to ask
about these.

Good luck!

(Disclaimer: All opinions are my own, not those of my employer.)

Kristine L. Danowski
Dow Chemical Company
Corporate R&D Analytical Sciences
1897 Building
Midland, MI 48667

voice -- 989-638-6912
fax -- 989-638-6027
email -- kldanowski-at-dow.com

The wisest man I ever knew taught me something I never forgot. And although
I never forgot it, I never quite memorized it either. So what I'm left with
is the memory of having learned something very wise that I can't quite
remember. -- George Carlin



From daemon Thu May 31 16:10:01 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Thu, 31 May 2001 17:07:23 -0400
Subject: CPD without intermediate fluid

Contents Retrieved from Microscopy Listserver Archives
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Has anyone here familiar with any attempts to achieve critical point drying
of a water saturated sample without the use of an intermediate fluid, i.e.,
by using the critical point of water itself at 374 oC/3212 PSI?

I'm interested in looking at clay samples. I am concerned that acetone will
cause structural rearrangement of the clay particles because of dissimilar
physical and electrical properties between acetone and water. Unlike
biological specimens, clay would not care the least about a temperature of
374 degrees, as it does not begin its dehydroxylization process until over
500 degrees. I work in an oil field related industry where we have ready
access to equipment that would consider 5000 PSI to be "light duty" so an
appropriate pressure bomb would not be difficult to construct.

Some questions:

1) All CPD devices that I have seen have a window that allows you to observe
the state of the meniscus. Is this essential, or can I assume that the
process will go as physics says it should without actually observing the
meniscus?

2) I do not want to place the clay samples in water and allow the water to
provide pressure as it is heated, as any water in contact with the clay will
start to decompose the sample. Can I use an external pressure source (high
pressure air, for example) to keep the pressure inside the bomb high enough
to avoid evaporation of any water until the critical point is reached? Would
it suffice to simply build a little platform that would keep the clay sample
above the water level?

3) Am I nuts for even considering this? What am I missing?

Thanks for your help.

Bruce Girrell
Microline Technology Corp.
2397 Traversefield Dr.
Traverse City, MI 49686
http://www.microlinetc.com

(231) 935-1585 (Voice)
(231) 922-5099 (FAX)
bigirrell-at-microlinetc.com



From daemon Thu May 31 17:20:53 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, May 31, 2001 6:39 AM
Subject: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
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Dr. Fabbri,

The following figures is a generalization to some degree, since I do not
know what brand of LaB6 cathode you are using.
LaB6 cathodes manufacturers advertise expected lifetime (hours) under vacuum
conditions of 10-7 mbar or better. When vacuum deteriorates closer to 10-6
mbar range, cathode lifetime will be reduced about twice. Generally, LaB6
cathodes are not recommended for use in vacuum (even intermittently) worse
than 10-6 mbar, as cathode lifetime reduces dramatically (exponentially).
Especially for LaB6 cathodes with metallic heater (versus Carbon one).

XL30 is a good instrument and must achieve much better vacuum than 6 x 10-7
mbar, continuously. Also, baking out may only improve vacuum once after
initial pump down in a system with negligible leaks. The fact that periodic
bake-out improves vacuum temporarily, may point to another condition, such
as micro leak(s). When parts of the gun/column are heated, materials do
expand, and leak(s) may (partially) close for awhile. Or, a substantial
amount of outgasing material is present in the UHV area. Or, vacuum readout
may be incorrect. In which case everything might be just fine!

All this is just a suggestion. A number of other scenarios possible, with a
number of proper troubleshooting techniques. If your SEM is still under
warranty, the solution is up to your service representative. His inspiration
is up to you.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: FABBRI {fabbri-at-cigssrv1.unimo.it}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}



We are using a quite new FEI XL-30 LaB6 SEM.
It's a long time the we are trying to understand the
normal working conditions of the vacuum system. Looking
at what is written in the operating manual, our vacuum system is not able
to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
To do this we need to bakeout the gun frequently ( weekly )and are able
to work in that safety ( { 2x10-7 )condition only for few hours.
FEI says that the info on the manual are too restrictive (!!) and that
we can work safetly with vacuum levels {6 x10-7 baking out every two weeks.

Any thoughts would be very welcome.
Thank you very much in advance.

Best regards
P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------








From daemon Thu May 31 19:10:16 2001



From: John V Nailon :      J.Nailon-at-mailbox.uq.edu.au
Date: Fri, 01 Jun 2001 10:06:22 +1000
Subject: Re: CPD without intermediate fluid

Contents Retrieved from Microscopy Listserver Archives
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G'day Bruce,
What is wrong in going the other way and simply dry your sample using a
small vacuum chamber??
High temperature and high pressure spell trouble to me.
Regards
JVN

Bruce Girrell wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Has anyone here familiar with any attempts to achieve critical point drying
} of a water saturated sample without the use of an intermediate fluid, i.e.,
} by using the critical point of water itself at 374 oC/3212 PSI?
}
} I'm interested in looking at clay samples. I am concerned that acetone will
} cause structural rearrangement of the clay particles because of dissimilar
} physical and electrical properties between acetone and water. Unlike
} biological specimens, clay would not care the least about a temperature of
} 374 degrees, as it does not begin its dehydroxylization process until over
} 500 degrees. I work in an oil field related industry where we have ready
} access to equipment that would consider 5000 PSI to be "light duty" so an
} appropriate pressure bomb would not be difficult to construct.
}
} Some questions:
}
} 1) All CPD devices that I have seen have a window that allows you to observe
} the state of the meniscus. Is this essential, or can I assume that the
} process will go as physics says it should without actually observing the
} meniscus?
}
} 2) I do not want to place the clay samples in water and allow the water to
} provide pressure as it is heated, as any water in contact with the clay will
} start to decompose the sample. Can I use an external pressure source (high
} pressure air, for example) to keep the pressure inside the bomb high enough
} to avoid evaporation of any water until the critical point is reached? Would
} it suffice to simply build a little platform that would keep the clay sample
} above the water level?
}
} 3) Am I nuts for even considering this? What am I missing?
}
} Thanks for your help.
}
} Bruce Girrell
} Microline Technology Corp.
} 2397 Traversefield Dr.
} Traverse City, MI 49686
} http://www.microlinetc.com
}
} (231) 935-1585 (Voice)
} (231) 922-5099 (FAX)
} bigirrell-at-microlinetc.com

--
John Nailon
Operations Manager
The Centre for Microscopy and Microanlaysis
The University of Queensland
St Lucia QLD 4072
Tel: +61-7-33654214
Fax: +61-7-33654422
WWW: http://www.uq.edu.au/nanoworld


From daemon Thu May 31 19:27:38 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 1 Jun 2001 10:24:51 +1000
Subject: RE: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


True, when the vacuum is marginal or when "accidents" occur, baking can save
the day. Although, there are limitations and eventually a cathodes will fail.
My point is that the performance of the established LaB6 brands are fairly
similar under ideal conditions, but Kimball's single center post design is
intrinsically more robust and will tolerate more abuse before failure. I
consider this is an established fact and is not an impression clouded by my
obvious commercial interest.
Disclaimer: ProSciTech supplies Kimball LaB6 (not to N America)
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, May 31, 2001 9:43 PM, Frank Thomas [SMTP:thomasf-at-AGC.BIO.NS.CA]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Dr. Fabbri -
}
} I take it from your message that the manual for the vacuum system is
} independent of the manual for the XL-30. I think I would tend to believe
} FEI. We operate our LaB6 routinely at 1 x 10-6.6 to 1 x 10-6.8 mbar. Our
} slightly poorer vacuum probably does result in somewhat shortened LaB6 life,
} but it's the best vacuum our aging ion pump can manage, and we rarely have
} "down" time because of vacuum problems. In a perfect vacuum, of course, the
} LaB6 filament would last a very, very long time, (theoretically); and the
} poorer the vacuum, the shorter the filament life. But I think in this case
} FEI is right - your vacuum system manual may be "too restrictive" for
} real-world operation. I would consider 6 x 10-7 an admirable vacuum, at
} least in our instrument. And, of course, FEI will be happy to sell you a new
} LaB6 when you need one ;-).
} If your new XL-30 is able to do its own "bake-out" routinely, then by all
} means use this function as often as you feel is necessary. And do keep an
} eye on the state of the vacuum - you may notice changes for the better or
} worse as time goes on.
} Good luck.
}
} F.C. Thomas
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada
}
}
}
} ----- Original Message -----
} } From: "FABBRI" {fabbri-at-cigssrv1.unimo.it}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, May 31, 2001 4:55 AM
} Subject: XL30 - LaB6 Vacuum system
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } We are using a quite new FEI XL-30 LaB6 SEM.
} } It's a long time the we are trying to understand the
} } normal working conditions of the vacuum system. Looking
} } at what is written in the operating manual, our vacuum system is not able
} } to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
} } To do this we need to bakeout the gun frequently ( weekly )and are able
} } to work in that safety ( { 2x10-7 )condition only for few hours.
} } FEI says that the info on the manual are too restrictive (!!) and that
} } we can work safetly with vacuum levels {6 x10-7 baking out every two
} weeks.
} }
} } Any thoughts would be very welcome.
} } Thank you very much in advance.
} }
} } Best regards
} } P.L. Fabbri
} }
} } ---------------------------------------------
} } Dr. Pier Luigi Fabbri
} } C.I.G.S. - Centro Interdip. Grandi Strumenti
} } Universita di Modena
} } via Campi 213/A - 41100 Modena, Italy
} } Tel +39-059-2055231 / +39-059-370551
} } Fax +39-059-370551
} } E-mail: fabbri-at-mail.cigs.unimo.it
} } ---------------------------------------------
} }
} }
} }
} }
} }



From daemon Thu May 31 19:56:12 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 31 May 2001 17:49:05 -0700
Subject: Re: 3Dreconstruction from 2D sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


AutoQuant makes a nice program called AutoVisualize.
It will take 2D slices and make them into a 3D image.

gary g.



At 03:59 PM 5/29/2001, you wrote:

} Kevin (and others),
}
} Your question about 3D reconstruction based on 2D sections was forwarded to my
} desk by an associate who is a member of your group.
}
} There are many reconstruction packages on the market. One of the ones I am
} aware of is the VoxBlast by VayTek (http://www.vaytek.com/VoxBlast.html).
}
} Another I have seen is called SpyGlass but I do not have a URL reference
} for that
} one.
}
} Best of luck.
}
} Matt
}
} Matthew M. Batchelor
} Applications Engineer
} ------------------------------------
} Meyer Instruments, Inc.
} 1304 Langham Creek, Suite 235
} Houston, TX 77084
} Video Microscopy Imaging Specialists
} P - 281-579-0342, F - 281-579-1551
} E-Mail = mmb-at-meyerinst.com
} Home Page = http://www.meyerinst.com/


Gary Gaugler, Ph.D.
Optical Reflections
7970 Twin Rocks Rd
Granite Bay, CA 95746-8111
916.791.8191
916.791.8186 fax

Disclaimer: I am an authorized dealer for this and other photographic
and software products.

http://photoweb.net




From daemon Fri Jun 1 02:26:24 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Fri, 01 Jun 2001 16:51:29 +0930
Subject: Osmium waste

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
Would anyone be able to tell me why corn oil is often recommended as the
correct oil to use to neutralise osmium tetroxide waste? Would any
vegetable oil do just as well, or does corn oil have special properties?
I have been unable to find it in supermarkets, and wonder if another
kind would be just as good.
Thanks.
Lyn Waterhouse
CEMMSA
Centre for Electron Microscopy
and Microstructure Analysis
University of Adelaide
Adelaide SA 5005
Ph: (08) 8303 4074
Fax: (08) 8303 4356
Website: http://www.adelaide.edu.au/CEMMSA


From daemon Fri Jun 1 02:32:53 2001



From: Tony Bruton :      bruton-at-nu.ac.za
Date: Fri, 01 Jun 2001 09:27:05 +0200
Subject: Re: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
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Dear XL30 LaB6 users

We have a LaB6 equipped ESEM installed in early 2000. It is still using the original filament which has now logged over 1000 hours. Although we have had various problems with the instrument vacuum levels have not been part of it. Gun vacuum is in the range 2.0 - 2.6 x10-7.

We bake it out overnight every time air is admitted to the gun for cleaning or repair. We are in the process of installing a high vac valve between the ion pump and the gun to save on bake outs at pressured times.

Sounds like you have a 'microleak' - get the agents onto it while you are still under warranty ! Baking out every two weeks might appeal to the purists who want to ensure 'ultimate vacuum' but it seems like overkill - unless it is compensating for a latent problem.

Another point that may be of interest - we have found that the instrument (with mixed lo and hivac usage) will last about three months before it starts getting wobbly ie gun instability and uncorrectable astigmatism. This is an indicator to clean the Wehnelt - itself no mean task since you are cleaning off an entirely transparent but totally nonconductive layer of Lanthanum (?) from the inside of the cap - checking for continuity with a multimeter as you clean. Once baked out after this process it performs like a star once again ! Thus far we are cleaning with polish, but are investigating less exhausting chemical cleaning methods !

The local service agents for FEI/Philips Anaspec-at-icon.co.za have assisted us a great deal in coming to terms with the idiosyncrasies of a LaB6 equipped ESEM - a concept which has given us some interesting moments.

Tony



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website via:http://www.nu.ac.za/department/default.asp?dept=cemunp
Email:bruton-at-nu.ac.za
postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } "FABBRI" {fabbri-at-cigssrv1.unimo.it} 05/31/01 09:55AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



We are using a quite new FEI XL-30 LaB6 SEM.
It's a long time the we are trying to understand the
normal working conditions of the vacuum system. Looking
at what is written in the operating manual, our vacuum system is not able
to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
To do this we need to bakeout the gun frequently ( weekly )and are able
to work in that safety ( { 2x10-7 )condition only for few hours.
FEI says that the info on the manual are too restrictive (!!) and that
we can work safetly with vacuum levels {6 x10-7 baking out every two weeks.

Any thoughts would be very welcome.
Thank you very much in advance.

Best regards
P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------







From daemon Fri Jun 1 05:18:15 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 1 Jun 2001 11:12:08 +0100 (GMT Daylight Time)
Subject: Re: CPD without intermediate fluid

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Access to cryoSEM or ESEM would save a lot of work.

(Without any real experience of clays) I would be inclined
to just air dry the sample at 40 deg. C in an oven.

The meniscus is required when replacing the intermediate
fluid, to prevent exposing the sample, since you would not
have one I guess it is irrelevant.

Dave


On Fri, 01 Jun 2001 10:06:22 +1000 John V Nailon
{J.Nailon-at-mailbox.uq.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} G'day Bruce,
} What is wrong in going the other way and simply dry your sample using a
} small vacuum chamber??
} High temperature and high pressure spell trouble to me.
} Regards
} JVN
}
} Bruce Girrell wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Has anyone here familiar with any attempts to achieve critical point drying
} } of a water saturated sample without the use of an intermediate fluid, i.e.,
} } by using the critical point of water itself at 374 oC/3212 PSI?
} }
} } I'm interested in looking at clay samples. I am concerned that acetone will
} } cause structural rearrangement of the clay particles because of dissimilar
} } physical and electrical properties between acetone and water. Unlike
} } biological specimens, clay would not care the least about a temperature of
} } 374 degrees, as it does not begin its dehydroxylization process until over
} } 500 degrees. I work in an oil field related industry where we have ready
} } access to equipment that would consider 5000 PSI to be "light duty" so an
} } appropriate pressure bomb would not be difficult to construct.
} }
} } Some questions:
} }
} } 1) All CPD devices that I have seen have a window that allows you to observe
} } the state of the meniscus. Is this essential, or can I assume that the
} } process will go as physics says it should without actually observing the
} } meniscus?
} }
} } 2) I do not want to place the clay samples in water and allow the water to
} } provide pressure as it is heated, as any water in contact with the clay will
} } start to decompose the sample. Can I use an external pressure source (high
} } pressure air, for example) to keep the pressure inside the bomb high enough
} } to avoid evaporation of any water until the critical point is reached? Would
} } it suffice to simply build a little platform that would keep the clay sample
} } above the water level?
} }
} } 3) Am I nuts for even considering this? What am I missing?
} }
} } Thanks for your help.
} }
} } Bruce Girrell
} } Microline Technology Corp.
} } 2397 Traversefield Dr.
} } Traverse City, MI 49686
} } http://www.microlinetc.com
} }
} } (231) 935-1585 (Voice)
} } (231) 922-5099 (FAX)
} } bigirrell-at-microlinetc.com
}
} --
} John Nailon
} Operations Manager
} The Centre for Microscopy and Microanlaysis
} The University of Queensland
} St Lucia QLD 4072
} Tel: +61-7-33654214
} Fax: +61-7-33654422
} WWW: http://www.uq.edu.au/nanoworld
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jun 1 07:40:56 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Fri, 1 Jun 2001 08:30:19 -0400
Subject: LM- Video camera/microscope coupler

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Johathan, I have the same Sony camera match on my Axiophot and Axiovert .
I have couple the cameras using optical couplers with a 0.6X magnification.
This overcomes some of the magnification factor and allows a much larger
section of the field to be captured. The couplers are made by Diagnostic
Instruments. If you want more info let me know.

Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com


-----Original Message-----
} From: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Thursday, May 31, 2001 4:00:PM
To: Microscopy-at-sparc5.microscopy.com


Hi:

A colleague wishes to get guidance and information about coupling his video
camera to his compound microscope.

He has a Sony DXC-970MD video camera. The camera has an ENG 1/2" bayonet
lens mount. He wants to use it on a Zeiss Axiophot.

I am used to simple C-mount adapters, the bayonet mount is the ringer for
me.

According to his research Sony offers an adapter, HRT045ENG12, that they
say is used to mount this camera to a microscope. His question is 'Does the
Sony adapter substitute for those offered by Zeiss for this purpose, or is
the Sony adapter used to convert the bayonet mount to something compatable
with the adapters offered by Zeiss?'

Finally, back in the old days, I remember learning that one could use a
simple T-mount to attach a 35 mm camera to a microscope, but that it was
not as good as having an eyepiece camera because it lacked the ocular lens.
Is the same true of mounting a video camera? I have always just used
simple, lenless C-mounts and it has seemed fine. If we are looking for
ultimate image quality, should we be using a more sophisticated adapter?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Jun 1 07:49:13 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Jun 2001 08:45:37 -0400
Subject: RE: LM- Video camera/microscope coupler

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jonathon,
Remember that the image projected by the ocular of an LM on the CCD
array is REAL ("Please draw a diagram of image formation in your light
microscope", he asked the students in the audience!). The lens in the 'C'
mounts are usually reduction lenses. [I haven't seen them all, so I am
depending on my long-held view that if one wanted more resolution, one would
use better objectives and 4x5" film(plates.] One may go without a reduction
lens on camera and adapter, but then the video image will only be a subset
[whose size (area) is dependent on the length of the 'C' adapter] of the
area that one 'sees' in the 'virtual' image space.
I have a Nikon bayonet-'C' adapter for a cooled CCD camera with a
built-in reduction lens. My purpose was to extend the camera for some macro
work using Nikon macro lenses that I already had for my Nikon 35mm camera.
I also have two different types of 'C' mount adapter. The lensLESS
adapter is used with a CCD camera that already has a reduction lens fitted
to it, while the lensED adapter is used with a vidicon that lacks a
reduction lens. I mismatched the adapters once and used the lensLESS
adapter on the lensLESS vidicon (did use the ocular!). Only a subset, and
no intensity!
The only way to make a decision is to try both Nikon and Zeiss
bayonet adapters, because one cannot determine - except empirically - what
design criteria the engineers used, though my suspicion would be that they
would not differ by much if both are used with an ocular. Also, watch the
length of the adapter. A little long, and the camera (I am not familiar
with its form.) may have to be connected more securely to avoid vibration
effects.

READ ON AT YOUR OWN RISK (OF WASTING TIME!)

Film Photomicrography?
How many listers have ever used a bellows camera setup for
photomicrography? Your age is determined by the thickness of the catalog
you received from the manufacturer of your microscope. What ARE we going to
do with all that stuff in the darkroom?

Leitz ORTHOLUX? Catalogs?
Speaking of that, I still have catalogs of Leitz (now Leica) from
the OrthoLUX epoch (1950's-1970+). If anyone needs something, a picture or
a number, don't hesitate to ask.

Video on TEM in 80's?
I remember an old TEM that I once used had a video (vidicon) camera
mounted under the viewing plate. The computer resided against half of a 9
foot wall, and was ROBUST. A user wanted to count mitochondria in the
cytoplasm of cells. The only way I could get a single mitochondrion in the
video camera view was to reduce the TEM mag almost to scan. So, while I
could see a large part of the grid on the EM view screen the camera
'grabbed' only the center of a grid square and didn't show a bar! To
program the computer, one had to remove boards and change jumpers. AH! The
good OLD days!

Regards to all,

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: jmkrupp-at-cats.ucsc.edu
} Sent: Thursday, May 31, 2001 3:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM- Video camera/microscope coupler
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} A colleague wishes to get guidance and information about coupling his
} video
} camera to his compound microscope.
}
} He has a Sony DXC-970MD video camera. The camera has an ENG 1/2" bayonet
} lens mount. He wants to use it on a Zeiss Axiophot.
}
} I am used to simple C-mount adapters, the bayonet mount is the ringer for
} me.
}
} According to his research Sony offers an adapter, HRT045ENG12, that they
} say is used to mount this camera to a microscope. His question is 'Does
} the
} Sony adapter substitute for those offered by Zeiss for this purpose, or is
} the Sony adapter used to convert the bayonet mount to something compatable
} with the adapters offered by Zeiss?'
}
} Finally, back in the old days, I remember learning that one could use a
} simple T-mount to attach a 35 mm camera to a microscope, but that it was
} not as good as having an eyepiece camera because it lacked the ocular
} lens.
} Is the same true of mounting a video camera? I have always just used
} simple, lenless C-mounts and it has seemed fine. If we are looking for
} ultimate image quality, should we be using a more sophisticated adapter?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}


From daemon Fri Jun 1 07:52:41 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 01 Jun 2001 08:41:37 -0400
Subject: Re: CPD without intermediate fluid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This reply is a bit off the Microscopy theme of the post, but here goes ---

Adding high pressure air to a "pressure bomb" will not prevent the
evaporation of the water (though it will slow it down). The vapor pressure
of any gas in equilibrium with its liquid phase is independent of the
pressure of other gases present. In Bruce's example, if he added air to
give a pressure of 3212psi at the critical temperature, the pressure in the
container would actually rise (assuming enough water was present) to
6424psi. This is why, when using a pressure cooker, you have to allow the
steam to vent for a few moments before closing the valve and allowing the
pressure to rise - the boiling point of the water depends only upon the
pressure of the water vapor above the liquid, not the total pressure.

Tony Garratt-Reed.



At 05:07 PM 5/31/2001 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri Jun 1 08:10:45 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 01 Jun 2001 09:06:43 -0400
Subject: Re: XL30 - LaB6 Vacuum system

Contents Retrieved from Microscopy Listserver Archives
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I don't have a LaB6 XL30, but I used to have its predecessor, a very early
ElectroScan E3. We ran that with LaB6 in a routine vacuum of about 5 -
7x10-7 Torr. It did not have the capability of being baked. The LaB6 ran
fine - we almost never were able to run filaments to their "natural" life,
as the manual control allowed students to abuse the current and emission
ratings - a facility they took advantage of regularly, destroying the
filaments in the process! However, we certainly had filaments run for up
to 1000 hrs.

As it aged (it was 10 years old when we replaced it, and still with its
original ion pump) we did not experience ultimate vacuum changes, although
the ion pump did become noticably harder to start after a gun vent.

As a general comment, I can't believe that a well-designed vacuum system
(as I assume the thermionic XL30 is), pumped by an ion pump and baked,
can't maintain a vacuum in the low -7's, unless there is a leak or some
other problem.

Tony G-R

} -----Original Message-----
} } From: FABBRI {fabbri-at-cigssrv1.unimo.it}
} To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}
} Date: Thursday, May 31, 2001 6:39 AM
} Subject: XL30 - LaB6 Vacuum system
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Fri Jun 1 08:18:40 2001



From: Edward_Principe-at-amat.com
Date: Fri, 1 Jun 2001 08:17:01 -0500
Subject: Re: Writing SOP's for SEM/X-Ray Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd like to echo the comments of Scott and Roger, who replied earlier. Their
wisdom is worth repeating. I wrote SOPs for a commercial analytical service
(not SEM specifically) in a past position........including the SOP on "writing
SOPs". Meet the requirements, but do not go overboard with too many
restrictions.

When considering a quality program it is so important to generate documentation
that you can adhere to and follow. It is possible to create an incredibly
"thorough" document which appears to cover every possible eventuality......but
is also impossible to execute. Quality is then not achieved. Those
who solicit
feedback and allow their quality program to evolve over time will have more
success. Having a strict internal review program that documents and corrects
existing problems is actually a benefit in terms of passing external reviews.
The internal documentation is typically the first thing examined by external
examiners and it places you in a better light if it is clear you are making an
effort to improve your program.

I would offer only a few suggestions for your specific analytical document:

If you have protocols for specific types of analyses that require more detailed
instruction incorporate these as separate work instructions that are distinct
from your SOP. The advantage is you can format your SOP in such a manner that
the WIs can be amended or additional WIs can be added, without having to
formerly generate an entire new version of your SOP. I have omitted the
details, but consult one of the many manuals offered by the host of officials
registrars. Referencing manuals is also fine for broader scope
descriptions and
procedures.

Maintanance Records and Statistical Process Control (SPC) procedures (i.e.,
calibration/sensitivity of EDS for example) are important components of your
quality documentation.

A certain amount (not all) of the quality program information for commercial
analytical service companies is available to customers (public). You may find
some useful information by requesting information from their quality manager
and/or asking specific departments or individuals about their quality
procedures.

Regards and Good Luck,
Ed Principe


From daemon Fri Jun 1 08:27:59 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Jun 2001 09:23:57 -0400
Subject: RE: TEM need help on embedding and cutting of ZnO crsytals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hope this helps.

Very old book, perhaps on someone's shelf (Old instructor of EM class)
Kay, D.H.,1965), Techniques for Electron Microscopy(2nd ed.),
Chapter 8, "Preparation of Thin Sections" (Glauert, A.M. and Phillips, R.),
Section: "Metallurgical and Crystallographic Applications of Thin
Sectioning", pp. 248-253 (includes references), F.A. Davis, Philadelphia,
PA, USA[Reprinted by Blackwell Scientific Pubs, Great Britain., 1967].

Refs: Fernandez-Moran, J. Biophys. biochem, Ctyol, 2(4), suppl
29(1956)
Reimer,L, Z.f.Metalkunde, 50, 37(1959)
Reimer, L, Naturwiss, 46, 68(1959)
Menter,M., Advances in Physics, 7, 229(1958)
Haanstra,H.B., Philips Technical Review 17, 101(1955)
Phillips, R., Aeon Laboratory Report FTR 1(1959)
Phillips, R., Aeon Laboratory Report No. 153(1960)

Kay, et al. promise that Zn is in there somewhere.

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Dujin Wang
} Sent: Thursday, May 31, 2001 3:54 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM need help on embedding and cutting of ZnO crsytals
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
} I am trying to do ultrathin sectioning work of ZnO crystals, which size
} is about 2-3microns(L) and 1-2microns(W). I am really a freshman
} on TEM, so I hope to get help on this work. Any suggestions for the
} choosing of embedding media and following procedures will be greatly
} appreciated.
} Thank you in advance.
}
} Dujin Wang
} Max-Planck-Institute for Polymer Research
} Ackermannweg 10, Mainz D-55128
} Germany
}
} Tel: 0049-6131-379226
} Fax: 0049-6131-379100
} Email: wangd-at-mpip-mainz.mpg.de
}
}
}


From daemon Fri Jun 1 08:58:24 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 1 Jun 2001 08:57:26 -0500
Subject: Re: Osmium waste

Contents Retrieved from Microscopy Listserver Archives
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Lyn

Corn oil is used because it has lots of double bonds for the osmium
to react with, so any highly unsaturated vegetable oil would do, or
even be better than corn oil.
Hey! Maybe there is a use for Vegemite ...

Phil

} Hello all,
} Would anyone be able to tell me why corn oil is often recommended as the
} correct oil to use to neutralise osmium tetroxide waste? Would any
} vegetable oil do just as well, or does corn oil have special properties?
} I have been unable to find it in supermarkets, and wonder if another
} kind would be just as good.
} Thanks.
} Lyn Waterhouse
} CEMMSA
} Centre for Electron Microscopy
} and Microstructure Analysis
} University of Adelaide
} Adelaide SA 5005
} Ph: (08) 8303 4074
} Fax: (08) 8303 4356
} Website: http://www.adelaide.edu.au/CEMMSA

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Jun 1 09:01:37 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 01 Jun 2001 09:57:56 -0400
Subject: RE: Writing SOP's for SEM/X-Ray Analysis

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This post was a few days ago - apologies for the delayed response, but I've
not seen others make quite the same points.

SOP's are merely recipes for achieving a goal. In Colin's case, he is
needing to achieve quality control, others may be needing safe operation,
ISO standard certification, or whatever. The point is that the SOP will
vary depending on the goal. There is no single "Standard Operating
Procedure" that covers every operating mode (unless it is so full of
qualifications, conditions, and alternatives as to be practically useless.)
For EDX, the manufacturer's instructions may be a good guide, and at least
a useful reference.

After stating the goal, the SOP will put down, in writing, all the
essential steps that must be followed to achieve this goal. This will
include such items as checking the performance of the system (mag. and beam
voltage calibration of the microscope, energy calibration and resolution of
the EDX, for example), as well as things like specimen preparation and
mounting (for quant. EDX, the specimen must be polished, and mounted in an
accurately known orientation, etc., etc.).

Hope this helps.

Tony Garratt-Reed


} -----Original Message-----
} Sent: Tuesday, May 29, 2001 1:20 AM
} To: MSA Listserver
} Subject: Writing SOP's for SEM/X-Ray Analysis
}
}
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
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** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
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From daemon Fri Jun 1 09:07:34 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 1 Jun 2001 23:55:10 +1000
Subject: RE: CPD without intermediate fluid

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You could freeze-dry specimens and that would be a rather more obvious
approach. The cost of building a high temperature/ pressure bomb aside the
question is: what would be the effect of the high temperature on the structure
of the clay.
I think what is missing from your consideration is that water is intrinsic to
clay and its nature is changed irreversible when all water is removed from its
chemical structure. I know that quite a few people have worked on the fine
structure of clay and it appears that this seemingly simple task is completely
elusive. I don't know, but I am doubtful that much useful could be gleaned from
modest SEM magnifications of dried clay.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, June 01, 2001 7:07 AM, Bruce Girrell
[SMTP:bigirrell-at-microlinetc.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Has anyone here familiar with any attempts to achieve critical point drying
} of a water saturated sample without the use of an intermediate fluid, i.e.,
} by using the critical point of water itself at 374 oC/3212 PSI?
}
} I'm interested in looking at clay samples. I am concerned that acetone will
} cause structural rearrangement of the clay particles because of dissimilar
} physical and electrical properties between acetone and water. Unlike
} biological specimens, clay would not care the least about a temperature of
} 374 degrees, as it does not begin its dehydroxylization process until over
} 500 degrees. I work in an oil field related industry where we have ready
} access to equipment that would consider 5000 PSI to be "light duty" so an
} appropriate pressure bomb would not be difficult to construct.
}
} Some questions:
}
} 1) All CPD devices that I have seen have a window that allows you to observe
} the state of the meniscus. Is this essential, or can I assume that the
} process will go as physics says it should without actually observing the
} meniscus?
}
} 2) I do not want to place the clay samples in water and allow the water to
} provide pressure as it is heated, as any water in contact with the clay will
} start to decompose the sample. Can I use an external pressure source (high
} pressure air, for example) to keep the pressure inside the bomb high enough
} to avoid evaporation of any water until the critical point is reached? Would
} it suffice to simply build a little platform that would keep the clay sample
} above the water level?
}
} 3) Am I nuts for even considering this? What am I missing?
}
} Thanks for your help.
}
} Bruce Girrell
} Microline Technology Corp.
} 2397 Traversefield Dr.
} Traverse City, MI 49686
} http://www.microlinetc.com
}
} (231) 935-1585 (Voice)
} (231) 922-5099 (FAX)
} bigirrell-at-microlinetc.com



From daemon Fri Jun 1 09:19:37 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Fri, 01 Jun 2001 10:17:12 -0400
Subject: Re: CPD without intermediate fluid

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Would you consider (freeze-fracturing, freeze-drying) or (freeze-drying, dry-fracturing) techniques? How about cryo-SEM and ESEM?
If you decide to build and use a super CPD, please let us know the results.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Bruce Girrell" {bigirrell-at-microlinetc.com} 05/31 5:07 PM } } }
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Has anyone here familiar with any attempts to achieve critical point drying
of a water saturated sample without the use of an intermediate fluid, i.e.,
by using the critical point of water itself at 374 oC/3212 PSI?

I'm interested in looking at clay samples. I am concerned that acetone will
cause structural rearrangement of the clay particles because of dissimilar
physical and electrical properties between acetone and water. Unlike
biological specimens, clay would not care the least about a temperature of
374 degrees, as it does not begin its dehydroxylization process until over
500 degrees. I work in an oil field related industry where we have ready
access to equipment that would consider 5000 PSI to be "light duty" so an
appropriate pressure bomb would not be difficult to construct.

Some questions:

1) All CPD devices that I have seen have a window that allows you to observe
the state of the meniscus. Is this essential, or can I assume that the
process will go as physics says it should without actually observing the
meniscus?

2) I do not want to place the clay samples in water and allow the water to
provide pressure as it is heated, as any water in contact with the clay will
start to decompose the sample. Can I use an external pressure source (high
pressure air, for example) to keep the pressure inside the bomb high enough
to avoid evaporation of any water until the critical point is reached? Would
it suffice to simply build a little platform that would keep the clay sample
above the water level?

3) Am I nuts for even considering this? What am I missing?

Thanks for your help.

Bruce Girrell
Microline Technology Corp.
2397 Traversefield Dr.
Traverse City, MI 49686
http://www.microlinetc.com

(231) 935-1585 (Voice)
(231) 922-5099 (FAX)
bigirrell-at-microlinetc.com





From daemon Fri Jun 1 09:28:48 2001



From: Carolyn.Gondran-at-SEMATECH.Org
Date: Fri, 01 Jun 2001 09:24:49 -0500
Subject: Materials Analysis Laboratory Manager Position Available at Inter

Contents Retrieved from Microscopy Listserver Archives
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} ******************************************************************************
} *************************************************
}
} International SEMATECH, a consortium of worldwide semiconductor manufacturing
} companies, has an opening for a Materials Analysis Laboratory Manager. We
} offer our employees an attractive package of benefits, including medical and
} dental coverage, group legal insurance, college tuition reimbursement, 12 paid
} holidays plus vacation. Additionally, we offer a generous retirement and
} shared savings plan, in which you are fully vested after two years of
} employment, PLUS a bonus program.
}
} To learn more about International SEMATECH, visit our website at
} www.sematech.org. To apply for the Materials Analysis Laboratory Manager
} position, send your resume to: staffing.hr-at-sematech.org. Principals only
} please.
}
}
} ******************************************************************************
} *****************
}
} Materials Analysis Laboratory Manager Job Description
} Job Summary:
} Management of the daily operation in the (Advanced Tool Development Facility)
} ATDF's Materials Analysis Laboratory in a cost effective method, including all
} logistical planning and scheduling activities. Continual interactions with the
} customer on the prioritizing and dispositioning of data and samples is a major
} component of this job.
} Job Responsibilities:
} * Maintain a safe working environment: be mindful of any potential
} hazards, report and/or correct anything that needs to be made safe,
} participate in monthly group self-inspections. Work with safety to update
} policies and procedures and ensure they are appropriate.
} * Maintain group specific safety training records. Manage technical
} leadership of group to "realize the roadmap"
} * Maintain a quality-staffing plan through guidance and feedback,
} encourage appropriate training, professional interaction, presentations and
} publications, retain (attract if needed) and develop quality staff.
} * Maintains & Reports on the MA lab's budget to both management and
} customers.
} * Ownership of MA indices and develops plans for continuous improvement of
} those indices
} * Promotes team interactions to optimize resources with sister FA lab
} * Provides the highest level of Customer satisfaction and Member Company
} value.
} * Maintain leading edge tool set through creative means, i.e. partnering,
} reduced cost of purchase, etc.
} Qualifications:
} * Minimum 4 years experience in management of an analytical laboratory in
} semiconductor industry
} * Current and comprehensive working knowledge of semiconductor
} manufacturing technology and associated characterization and metrology
} equipment and techniques
} * Direct semiconductor industry experience with all or most of the
} following techniques: Auger, SIMS-ICP-MS, TEM, SEM, FIB/SIM, AFM, TXRF, and
} VPD-ICP-MS
} * Degree in: Material Sciences, Surface Chemistry, Physics or Chemical
} Engineering preferred, PhD, MS, EE and/or BS with commensurate experience.
} * Currently Published articles in related field(s)
} Special Conditions:
} * Travel Required {10% of the time.
} * Shift/Hours worked:1st
}


From daemon Fri Jun 1 10:49:57 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 01 Jun 2001 11:46:20 -0500
Subject: Osmium Recycling

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Lyn Waterhouse wrote:
=================================================
Would anyone be able to tell me why corn oil is often recommended as the
correct oil to use to neutralise osmium tetroxide waste? Would any vegetable
oil do just as well, or does corn oil have special properties? I have been
unable to find it in supermarkets, and wonder if another kind would be just
as good.
=============================================
Whether one kind of oil works better vs. another is a function of the
unsaturation present. However, from an environmental and also a recycling
standpoint, none of these "oils" is very environmentally friendly because
once the osmium is put into this state, the economics of recovery become so
terrible, that the concept of recovery and recycling becomes impossible (at
least at current market prices). The only fate is incineration and burial
in a land fill.

SPI Supplies is now beta testing our own SPI Osmium Recyling Kit. It
consists of a 4 liter bottle with some formulated "potion" inside. When the
bottle is filled up, the idea is for it to be returned to SPI Supplies for
recycling. The chemistry is not rocket scientist logic, but navigating the
various regulatory challenges, especially since they tend to vary from
country to country, not to mention the rules being constantly changing as
well, is what presents the real challenges. We believe the economics are
such that we could even "give back" some new osmium tetroxide in amoules
upon the return of such a bottle.

We would be happy to send you, with our compliments, a beta test kit. Just
let us know if you would like us to do that. We would give you instructions
for returning it to SPI for recycling. We are doing this on a carefully
controlled basis for now, because every time we think we have all the bases
covered, we encounter some other surprise. However, we plan to make some
kind of more formal announcement about it on our website later this year, if
not earlier at the M&M meeting, when the kit is officially introduced as a
regular product of SPI Supplies.

But in the mean time, rather than doing the neutralization in an "oil", use
1N KOH solution. You will get the final reduction of the tetroxide to the
dioxide, and the resulting system could at some future time, literally be
"added" to the SPI Osmium Recycling Kit when it is more widely available.
Now this suggestion might not be right for everyone but it is an alternative
to rendering the material to a fate outside of the stream of commerce from
which it is then lost for the benefit of future generations.

Disclaimer: SPI Supplies would like to promote the concept of recycling
instead of incineration and burial of this non-renewable resource. This is
called "passionate commercialism", something I hope is permitted on this
listserver......!

Chuck

===============================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Fri Jun 1 11:19:47 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 1 Jun 2001 12:18:24 -0400
Subject: Osmium Recycling

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Chuck,

What volume ratio of 0.1 M KOH to OsO4 (say, 1% in buffer) do you recommend?

Marie

You wrote:

} But in the mean time, rather than doing the neutralization in an "oil", use
} 1N KOH solution.

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Fri Jun 1 13:41:19 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Fri, 01 Jun 2001 16:51:29 +0930
Subject: Osmium waste

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Hello all,
Would anyone be able to tell me why corn oil is often recommended as the
correct oil to use to neutralise osmium tetroxide waste? Would any
vegetable oil do just as well, or does corn oil have special properties?
I have been unable to find it in supermarkets, and wonder if another
kind would be just as good.
Thanks.
Lyn Waterhouse
CEMMSA
Centre for Electron Microscopy
and Microstructure Analysis
University of Adelaide
Adelaide SA 5005
Ph: (08) 8303 4074
Fax: (08) 8303 4356
Website: http://www.adelaide.edu.au/CEMMSA



From daemon Fri Jun 1 13:44:07 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 01 Jun 2001 08:41:37 -0400
Subject: Re: CPD without intermediate fluid

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This reply is a bit off the Microscopy theme of the post, but here goes ---

Adding high pressure air to a "pressure bomb" will not prevent the
evaporation of the water (though it will slow it down). The vapor pressure
of any gas in equilibrium with its liquid phase is independent of the
pressure of other gases present. In Bruce's example, if he added air to
give a pressure of 3212psi at the critical temperature, the pressure in the
container would actually rise (assuming enough water was present) to
6424psi. This is why, when using a pressure cooker, you have to allow the
steam to vent for a few moments before closing the valve and allowing the
pressure to rise - the boiling point of the water depends only upon the
pressure of the water vapor above the liquid, not the total pressure.

Tony Garratt-Reed.



At 05:07 PM 5/31/2001 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Fri Jun 1 13:45:18 2001



From: Tony Bruton :      bruton-at-nu.ac.za
Date: Fri, 01 Jun 2001 09:27:05 +0200
Subject: Re: XL30 - LaB6 Vacuum system

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Dear XL30 LaB6 users

We have a LaB6 equipped ESEM installed in early 2000. It is still using the original filament which has now logged over 1000 hours. Although we have had various problems with the instrument vacuum levels have not been part of it. Gun vacuum is in the range 2.0 - 2.6 x10-7.

We bake it out overnight every time air is admitted to the gun for cleaning or repair. We are in the process of installing a high vac valve between the ion pump and the gun to save on bake outs at pressured times.

Sounds like you have a 'microleak' - get the agents onto it while you are still under warranty ! Baking out every two weeks might appeal to the purists who want to ensure 'ultimate vacuum' but it seems like overkill - unless it is compensating for a latent problem.

Another point that may be of interest - we have found that the instrument (with mixed lo and hivac usage) will last about three months before it starts getting wobbly ie gun instability and uncorrectable astigmatism. This is an indicator to clean the Wehnelt - itself no mean task since you are cleaning off an entirely transparent but totally nonconductive layer of Lanthanum (?) from the inside of the cap - checking for continuity with a multimeter as you clean. Once baked out after this process it performs like a star once again ! Thus far we are cleaning with polish, but are investigating less exhausting chemical cleaning methods !

The local service agents for FEI/Philips Anaspec-at-icon.co.za have assisted us a great deal in coming to terms with the idiosyncrasies of a LaB6 equipped ESEM - a concept which has given us some interesting moments.

Tony



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website via:http://www.nu.ac.za/department/default.asp?dept=cemunp
Email:bruton-at-nu.ac.za
postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } "FABBRI" {fabbri-at-cigssrv1.unimo.it} 05/31/01 09:55AM } } }
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We are using a quite new FEI XL-30 LaB6 SEM.
It's a long time the we are trying to understand the
normal working conditions of the vacuum system. Looking
at what is written in the operating manual, our vacuum system is not able
to work in safety conditions for the Lab6 filament ( { 2x10-7 mbar ).
To do this we need to bakeout the gun frequently ( weekly )and are able
to work in that safety ( { 2x10-7 )condition only for few hours.
FEI says that the info on the manual are too restrictive (!!) and that
we can work safetly with vacuum levels {6 x10-7 baking out every two weeks.

Any thoughts would be very welcome.
Thank you very much in advance.

Best regards
P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------








From daemon Fri Jun 1 13:51:49 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 1 Jun 2001 11:12:08 +0100 (GMT Daylight Time)
Subject: Re: CPD without intermediate fluid

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Access to cryoSEM or ESEM would save a lot of work.

(Without any real experience of clays) I would be inclined
to just air dry the sample at 40 deg. C in an oven.

The meniscus is required when replacing the intermediate
fluid, to prevent exposing the sample, since you would not
have one I guess it is irrelevant.

Dave


On Fri, 01 Jun 2001 10:06:22 +1000 John V Nailon
{J.Nailon-at-mailbox.uq.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} G'day Bruce,
} What is wrong in going the other way and simply dry your sample using a
} small vacuum chamber??
} High temperature and high pressure spell trouble to me.
} Regards
} JVN
}
} Bruce Girrell wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Has anyone here familiar with any attempts to achieve critical point drying
} } of a water saturated sample without the use of an intermediate fluid, i.e.,
} } by using the critical point of water itself at 374 oC/3212 PSI?
} }
} } I'm interested in looking at clay samples. I am concerned that acetone will
} } cause structural rearrangement of the clay particles because of dissimilar
} } physical and electrical properties between acetone and water. Unlike
} } biological specimens, clay would not care the least about a temperature of
} } 374 degrees, as it does not begin its dehydroxylization process until over
} } 500 degrees. I work in an oil field related industry where we have ready
} } access to equipment that would consider 5000 PSI to be "light duty" so an
} } appropriate pressure bomb would not be difficult to construct.
} }
} } Some questions:
} }
} } 1) All CPD devices that I have seen have a window that allows you to observe
} } the state of the meniscus. Is this essential, or can I assume that the
} } process will go as physics says it should without actually observing the
} } meniscus?
} }
} } 2) I do not want to place the clay samples in water and allow the water to
} } provide pressure as it is heated, as any water in contact with the clay will
} } start to decompose the sample. Can I use an external pressure source (high
} } pressure air, for example) to keep the pressure inside the bomb high enough
} } to avoid evaporation of any water until the critical point is reached? Would
} } it suffice to simply build a little platform that would keep the clay sample
} } above the water level?
} }
} } 3) Am I nuts for even considering this? What am I missing?
} }
} } Thanks for your help.
} }
} } Bruce Girrell
} } Microline Technology Corp.
} } 2397 Traversefield Dr.
} } Traverse City, MI 49686
} } http://www.microlinetc.com
} }
} } (231) 935-1585 (Voice)
} } (231) 922-5099 (FAX)
} } bigirrell-at-microlinetc.com
}
} --
} John Nailon
} Operations Manager
} The Centre for Microscopy and Microanlaysis
} The University of Queensland
} St Lucia QLD 4072
} Tel: +61-7-33654214
} Fax: +61-7-33654422
} WWW: http://www.uq.edu.au/nanoworld
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Fri Jun 1 14:05:43 2001



From: Pranatharthi, Balasubraman :      pranatha-at-engr.sc.edu
Date: Fri, 1 Jun 2001 14:57:26 -0400
Subject: Query on Accelerating Voltage

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Hi !!!
I was doing EDAX on a sample to determine the surface constituents. Can you
let me know answers to the following questions:
1. When I vary the accelerating voltage the atomic % (concentration of = }
elements) varies. Why is that?
2. What is the depth of penetration of EDAX? Does this depend on = }
accelerating voltage?
Please send copies of your reply to bala-at-sc.edu.
Thanks in advance
Sincerely,
Bala Haran



From daemon Fri Jun 1 14:05:44 2001



From: Pranatharthi, Balasubraman :      pranatha-at-engr.sc.edu
Date: Fri, 1 Jun 2001 14:57:26 -0400
Subject: Query on Accelerating Voltage

Contents Retrieved from Microscopy Listserver Archives
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Hi !!!
I was doing EDAX on a sample to determine the surface constituents. Can you
let me know answers to the following questions:
1. When I vary the accelerating voltage the atomic % (concentration of = }
elements) varies. Why is that?
2. What is the depth of penetration of EDAX? Does this depend on = }
accelerating voltage?
Please send copies of your reply to bala-at-sc.edu.
Thanks in advance
Sincerely,
Bala Haran



From daemon Fri Jun 1 14:48:59 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 01 Jun 2001 12:40:05 -0700
Subject: Re: LM- Video camera/microscope coupler

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Optem and Diagnostic Instrument make adapters for this
camera to most any microscope.

gary g.


At 12:59 PM 5/31/2001, you wrote:

} Hi:
}
} A colleague wishes to get guidance and information about coupling his video
} camera to his compound microscope.
}
} He has a Sony DXC-970MD video camera. The camera has an ENG 1/2" bayonet
} lens mount. He wants to use it on a Zeiss Axiophot.
}
} I am used to simple C-mount adapters, the bayonet mount is the ringer for me.
}
} According to his research Sony offers an adapter, HRT045ENG12, that they
} say is used to mount this camera to a microscope. His question is 'Does the
} Sony adapter substitute for those offered by Zeiss for this purpose, or is
} the Sony adapter used to convert the bayonet mount to something compatable
} with the adapters offered by Zeiss?'
}
} Finally, back in the old days, I remember learning that one could use a
} simple T-mount to attach a 35 mm camera to a microscope, but that it was
} not as good as having an eyepiece camera because it lacked the ocular lens.
} Is the same true of mounting a video camera? I have always just used
} simple, lenless C-mounts and it has seemed fine. If we are looking for
} ultimate image quality, should we be using a more sophisticated adapter?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu



From daemon Fri Jun 1 15:32:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 1 Jun 2001 08:45:37 -0400
Subject: RE: LM- Video camera/microscope coupler

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Hi Jonathon,
Remember that the image projected by the ocular of an LM on the CCD
array is REAL ("Please draw a diagram of image formation in your light
microscope", he asked the students in the audience!). The lens in the 'C'
mounts are usually reduction lenses. [I haven't seen them all, so I am
depending on my long-held view that if one wanted more resolution, one would
use better objectives and 4x5" film(plates.] One may go without a reduction
lens on camera and adapter, but then the video image will only be a subset
[whose size (area) is dependent on the length of the 'C' adapter] of the
area that one 'sees' in the 'virtual' image space.
I have a Nikon bayonet-'C' adapter for a cooled CCD camera with a
built-in reduction lens. My purpose was to extend the camera for some macro
work using Nikon macro lenses that I already had for my Nikon 35mm camera.
I also have two different types of 'C' mount adapter. The lensLESS
adapter is used with a CCD camera that already has a reduction lens fitted
to it, while the lensED adapter is used with a vidicon that lacks a
reduction lens. I mismatched the adapters once and used the lensLESS
adapter on the lensLESS vidicon (did use the ocular!). Only a subset, and
no intensity!
The only way to make a decision is to try both Nikon and Zeiss
bayonet adapters, because one cannot determine - except empirically - what
design criteria the engineers used, though my suspicion would be that they
would not differ by much if both are used with an ocular. Also, watch the
length of the adapter. A little long, and the camera (I am not familiar
with its form.) may have to be connected more securely to avoid vibration
effects.

READ ON AT YOUR OWN RISK (OF WASTING TIME!)

Film Photomicrography?
How many listers have ever used a bellows camera setup for
photomicrography? Your age is determined by the thickness of the catalog
you received from the manufacturer of your microscope. What ARE we going to
do with all that stuff in the darkroom?

Leitz ORTHOLUX? Catalogs?
Speaking of that, I still have catalogs of Leitz (now Leica) from
the OrthoLUX epoch (1950's-1970+). If anyone needs something, a picture or
a number, don't hesitate to ask.

Video on TEM in 80's?
I remember an old TEM that I once used had a video (vidicon) camera
mounted under the viewing plate. The computer resided against half of a 9
foot wall, and was ROBUST. A user wanted to count mitochondria in the
cytoplasm of cells. The only way I could get a single mitochondrion in the
video camera view was to reduce the TEM mag almost to scan. So, while I
could see a large part of the grid on the EM view screen the camera
'grabbed' only the center of a grid square and didn't show a bar! To
program the computer, one had to remove boards and change jumpers. AH! The
good OLD days!

Regards to all,

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: jmkrupp-at-cats.ucsc.edu
} Sent: Thursday, May 31, 2001 3:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM- Video camera/microscope coupler
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} A colleague wishes to get guidance and information about coupling his
} video
} camera to his compound microscope.
}
} He has a Sony DXC-970MD video camera. The camera has an ENG 1/2" bayonet
} lens mount. He wants to use it on a Zeiss Axiophot.
}
} I am used to simple C-mount adapters, the bayonet mount is the ringer for
} me.
}
} According to his research Sony offers an adapter, HRT045ENG12, that they
} say is used to mount this camera to a microscope. His question is 'Does
} the
} Sony adapter substitute for those offered by Zeiss for this purpose, or is
} the Sony adapter used to convert the bayonet mount to something compatable
} with the adapters offered by Zeiss?'
}
} Finally, back in the old days, I remember learning that one could use a
} simple T-mount to attach a 35 mm camera to a microscope, but that it was
} not as good as having an eyepiece camera because it lacked the ocular
} lens.
} Is the same true of mounting a video camera? I have always just used
} simple, lenless C-mounts and it has seemed fine. If we are looking for
} ultimate image quality, should we be using a more sophisticated adapter?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}



From daemon Fri Jun 1 15:35:47 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Fri, 01 Jun 2001 16:51:29 +0930
Subject: Osmium waste

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Hello all,
Would anyone be able to tell me why corn oil is often recommended as the
correct oil to use to neutralise osmium tetroxide waste? Would any
vegetable oil do just as well, or does corn oil have special properties?
I have been unable to find it in supermarkets, and wonder if another
kind would be just as good.
Thanks.
Lyn Waterhouse
CEMMSA
Centre for Electron Microscopy
and Microstructure Analysis
University of Adelaide
Adelaide SA 5005
Ph: (08) 8303 4074
Fax: (08) 8303 4356
Website: http://www.adelaide.edu.au/CEMMSA



From daemon Fri Jun 1 16:09:46 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Fri, 01 Jun 2001 17:03:12 -0400
Subject: autofluorescence - how specific is it?

Contents Retrieved from Microscopy Listserver Archives
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Staining cardiac vessel (e.g. aorta) wall is accompanied by a lot of autofluorescence, probably from elastin and perhaps other extracellualr matrix material.

Should attempts to reduce autofluorescence concentrate on the source of the problem, in other words, are there specific treatments for different types, such as lipofuscin versus elastin.

I have tried Sudan black B, sodium borohydride, toluidine blue. The first 2 reduced the red wavelength contribution only while toluidine blue actually increased the red autofluorescence. The green signal was reduced by only a small amount, all compared to just a PBS wash.

Any suggestions would be highly appreciated.



Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Fri Jun 1 17:40:34 2001



From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 1 Jun 2001 17:35:12 -0500
Subject: Re: Query on Accelerating Voltage

Contents Retrieved from Microscopy Listserver Archives
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Bala

Have a look at the following www site

http://tpm.amc.anl.gov/Lectures

download the PDF file "Introduction to XEDS for the SEM"

Answers to some of your questions will be in there.

Variation in the composition with Accelerating
Voltage is due to the Cross-section changing with kV


There are also a few slides on spatial resolution.

You should however, get a text book on Microanalysis
and read it in detail.


Nestor
Your Friendly Neighborhood SysOp




} ------------------------------------------------------------------------
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--
===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4289
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From daemon Fri Jun 1 19:30:47 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 1 Jun 2001 11:12:08 +0100 (GMT Daylight Time)
Subject: Re: CPD without intermediate fluid

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Access to cryoSEM or ESEM would save a lot of work.

(Without any real experience of clays) I would be inclined
to just air dry the sample at 40 deg. C in an oven.

The meniscus is required when replacing the intermediate
fluid, to prevent exposing the sample, since you would not
have one I guess it is irrelevant.

Dave


On Fri, 01 Jun 2001 10:06:22 +1000 John V Nailon
{J.Nailon-at-mailbox.uq.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} G'day Bruce,
} What is wrong in going the other way and simply dry your sample using a
} small vacuum chamber??
} High temperature and high pressure spell trouble to me.
} Regards
} JVN
}
} Bruce Girrell wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Has anyone here familiar with any attempts to achieve critical point drying
} } of a water saturated sample without the use of an intermediate fluid, i.e.,
} } by using the critical point of water itself at 374 oC/3212 PSI?
} }
} } I'm interested in looking at clay samples. I am concerned that acetone will
} } cause structural rearrangement of the clay particles because of dissimilar
} } physical and electrical properties between acetone and water. Unlike
} } biological specimens, clay would not care the least about a temperature of
} } 374 degrees, as it does not begin its dehydroxylization process until over
} } 500 degrees. I work in an oil field related industry where we have ready
} } access to equipment that would consider 5000 PSI to be "light duty" so an
} } appropriate pressure bomb would not be difficult to construct.
} }
} } Some questions:
} }
} } 1) All CPD devices that I have seen have a window that allows you to observe
} } the state of the meniscus. Is this essential, or can I assume that the
} } process will go as physics says it should without actually observing the
} } meniscus?
} }
} } 2) I do not want to place the clay samples in water and allow the water to
} } provide pressure as it is heated, as any water in contact with the clay will
} } start to decompose the sample. Can I use an external pressure source (high
} } pressure air, for example) to keep the pressure inside the bomb high enough
} } to avoid evaporation of any water until the critical point is reached? Would
} } it suffice to simply build a little platform that would keep the clay sample
} } above the water level?
} }
} } 3) Am I nuts for even considering this? What am I missing?
} }
} } Thanks for your help.
} }
} } Bruce Girrell
} } Microline Technology Corp.
} } 2397 Traversefield Dr.
} } Traverse City, MI 49686
} } http://www.microlinetc.com
} }
} } (231) 935-1585 (Voice)
} } (231) 922-5099 (FAX)
} } bigirrell-at-microlinetc.com
}
} --
} John Nailon
} Operations Manager
} The Centre for Microscopy and Microanlaysis
} The University of Queensland
} St Lucia QLD 4072
} Tel: +61-7-33654214
} Fax: +61-7-33654422
} WWW: http://www.uq.edu.au/nanoworld
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Sat Jun 2 00:30:42 2001



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Sat, 2 Jun 2001 12:23:49 +0700
Subject: out of office

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers!
Thank you very much for your interest in our problems.
I got yours recommendation for HT check. Thank you very much again.
Next week I will be in Moscow and I will be back on Friday June 8,
2001.
Happy week end.
Best regards
Anton
mailto:gut-at-thermo.isp.nsc.ru



From daemon Sun Jun 3 09:45:53 2001



From: alsamszw-at-aol.com ()
Date: Sun, 3 Jun 2001 09:32:28 -0500
Subject: Ask-A-Microscopist: 3D microscopy and has this been used to study

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(alsamszw-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
June 2, 2001 at 15:48:55
---------------------------------------------------------------------------

Email: alsamszw-at-aol.com
Name: Dr S Z AL-SAM

Organization: Mid Essex Hospitals, Chelmsford, UK

Education: Graduate College

Location: Chelmsford, United Kingdom

Question: Please tell me more about 3D microscopy and has this been
used to study cytological smears before. Who supply these
microscopes and how much do they cost?
Many thanks.

---------------------------------------------------------------------------


From daemon Sun Jun 3 09:45:58 2001



From: Alsamszw-at-aol.com
Date: Sun, 3 Jun 2001 09:30:25 -0500
Subject: Ask-A-Microscopist: 3D microscopes?

Contents Retrieved from Microscopy Listserver Archives
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Please tell me more about 3D images and 3D microscopes. Have these been used
to study cytological smears and histological sections? How much do they cost
and are there catalogues available? thank you
AL-SAM


From daemon Mon Jun 4 04:44:45 2001



From: Keith Ryan :      KPRyan-at-pml.ac.uk
Date: Mon, 4 Jun 2001 10:36:34 +0100
Subject: Tech. job available

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Hello Listers

I have been asked to post this job by colleagues.

Keith Ryan
MBA
Plymouth, UK

___________________________________________________

MARINE BIOLOGICAL ASSOCIATION OF THE UK

Research Technician (part-time) Electron Microscopy and Biochemistry.

We are seeking a technician preferably with experience in biochemistry and/or electron microscopy to join this BBSRC funded project “Trans-cellular Ca2+ transport and Ca2+ homeostasis in calcifying microalgae”. The post holder will be responsible for preparation and analysis of algal cells using electron microscopy in addition to purification of calcium binding proteins using gel electrophoresis.

The post is offered as a 50% fixed term appointment for 18 months with starting salary £13,717 (pro-rata), although other suitable working arrangements will be considered.

For further details of this project you can visit the following website: http://www.mba.ac.uk

Informal enquires may be addressed to Dr Alison Taylor (tel: +44 (0)1752 633290,
e-mail: arta-at-mba.ac.uk) or Professor Colin Brownlee (tel: +44 (0)1752 633246,
e-mail: cbr-at-wpo.nerc.ac.uk).

Closing Date: open until appointed but start by November 2001








From daemon Mon Jun 4 08:38:27 2001



From: Richard Cole :      rcole-at-wadsworth.org
Date: Mon, 4 Jun 2001 09:30:48 -0400
Subject: EM Tech position available

Contents Retrieved from Microscopy Listserver Archives
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Dear Group


Job Announcement:

Electron Microscopy(full time, permanent) Technician —Health Research Inc.,
Wadsworth Center, Albany, NY. We are looking for a motivated, mature and
responsible individual to join a state-of-the-art Federal funded biomedical
research laboratory. Flexible job hours in a challenging and stimulating
research environment. Will train for specialized tasks.


Minimum qualification: B.S. in biology or related filed.

Preferred qualification: B.S. with experience in specimen sectioning and
electron microscopy.

Responsibilities:
1. Embedding various biological specimens for transmission electron
microscopy analyses.
2. Thin and thick (serially) sectioning of specimens.
3. Scanning and photographing sections with the EM
4. Conducting 3D analyses.





Contact:
Richard Cole
Research Scientist III
Director of the Laser Microsurgery and Advanced Light Microscopy Core Unit
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
rcole-at-wadsworth.org
518-474-7048 Phone
518-486-4901 Fax



From daemon Mon Jun 4 10:14:22 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 4 Jun 2001 08:01:58 -0700 (PDT)
Subject: Re: autofluorescence - how specific is it?

Contents Retrieved from Microscopy Listserver Archives
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Hi Judy,

We do alot of Immunofluorescence on sections of human skin. The main
source of atuofluorescence is the elastin in the dermis and concentrated
around the larger vessels. The best success we have had is using a CY5
label. The autofluorescence for elastin goes down dramaticly in the longer
wavelengths. Or if we are using a single antiboby, we cature an image of
the autofluorescence in a different channel and digitally subtract it out
of the final image using image math. Then actually, most of the time we
just leave it in and acknowledge that it is elastin (it is actually a good
structural landmark and quite beautiful).

Bob
Dermatology Research Center
U of Washington, Seattle

On Fri, 1 Jun 2001, Judy Trogadis wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Staining cardiac vessel (e.g. aorta) wall is accompanied by a lot of autofluorescence, probably from elastin and perhaps other extracellualr matrix material.
}
} Should attempts to reduce autofluorescence concentrate on the source of the problem, in other words, are there specific treatments for different types, such as lipofuscin versus elastin.
}
} I have tried Sudan black B, sodium borohydride, toluidine blue. The first 2 reduced the red wavelength contribution only while toluidine blue actually increased the red autofluorescence. The green signal was reduced by only a small amount, all compared to just a PBS wash.
}
} Any suggestions would be highly appreciated.
}
}
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital
} 30 Bond St.
} Toronto, ON M5B 1W8
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
}
}



From daemon Mon Jun 4 12:31:29 2001



From: Karen Dye :      karen.dye-at-medtronic.com
Date: Mon, 04 Jun 2001 12:25:05 -0500
Subject: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?



From daemon Mon Jun 4 12:39:04 2001



From: Karen Dye :      karen.dye-at-medtronic.com
Date: Mon, 04 Jun 2001 12:35:06 -0500
Subject: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?



From daemon Mon Jun 4 12:47:49 2001



From: Earl Godfrey :      godfreew-at-borg.evms.edu
Date: Mon, 4 Jun 2001 13:41:53 -0400
Subject: Confocal LM/EM Imaging Core Director Faculty Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DIRECTOR, CELLULAR IMAGING CENTER: The Department of Pathology and Anatomy
seeks Ph.D. applicants for a faculty position in fall 2001 as director of
our cellular imaging core facility. The facility is equipped with
transmission and scanning electron microscopes, a new laser scanning
confocal microscope, image analysis computer, and conventional fluorescence
microscopes.. Strong credentials in state of the art light and/or electron
microscopy (e.g., confocal applications, FRET) are desired. In addition to
overseeing the imaging core facility, the director will be expected to
develop independent and/or collaborative research in one of five research
areas: Cancer, Cardiovascular/Renal, Neuroscience, Reproductive
Endocrinology, and Virology. Please send curriculum vitae, statement of
research interests, and names, addresses, telephone numbers and email
addresses of three references to:

William F. Glass II, M.D. Ph.D., Chair
Department of Pathology and Anatomy
Eastern Virginia Medical School
P.O. Box 1980
Norfolk, VA 23501

Eastern Virginia Medical School is an Affirmative Action/Equal Opportunity
Employer.

Questions may be directed to Dr. Glass or Dr. Earl Godfrey
(godfreew-at-borg.evms.edu).

Please inform qualified individuals of this opportunity. Thank you very much.


Earl W. Godfrey, Ph.D.
Dept. of Pathology and Anatomy
Eastern Virginia Medical School
P.O. Box 1980
Norfolk, VA 23501

street address: 700 W. Olney Rd.
Lewis Hall Rm. 3077a
Norfolk, VA 23507

(757) 446-5609
FAX (757) 446-5719
Email: godfreew-at-borg.evms.edu





From daemon Mon Jun 4 12:50:23 2001



From: Karen Dye :      karen.dye-at-medtronic.com
Date: Mon, 04 Jun 2001 12:46:37 -0500
Subject: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?



From daemon Mon Jun 4 12:55:38 2001



From: Karen Dye :      karen.dye-at-medtronic.com
Date: Mon, 04 Jun 2001 12:51:29 -0500
Subject: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?



From daemon Mon Jun 4 13:51:46 2001



From: Earl Godfrey :      godfreew-at-borg.evms.edu
Date: Mon, 4 Jun 2001 14:44:31 -0400
Subject: Confocal LM/EM Imaging Core Director Faculty Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DIRECTOR, CELLULAR IMAGING CENTER: The Department of Pathology and Anatomy
seeks Ph.D. applicants for a faculty position in fall 2001 as director of
our cellular imaging core facility. The facility is equipped with
transmission and scanning electron microscopes, a new laser scanning
confocal microscope, image analysis computer, and conventional fluorescence
microscopes.. Strong credentials in state of the art light and/or electron
microscopy (e.g., confocal applications, FRET) are desired. In addition to
overseeing the imaging core facility, the director will be expected to
develop independent and/or collaborative research in one of five research
areas: Cancer, Cardiovascular/Renal, Neuroscience, Reproductive
Endocrinology, and Virology. Please send curriculum vitae, statement of
research interests, and names, addresses, telephone numbers and email
addresses of three references to:

William F. Glass II, M.D. Ph.D., Chair
Department of Pathology and Anatomy
Eastern Virginia Medical School
P.O. Box 1980
Norfolk, VA 23501

Eastern Virginia Medical School is an Affirmative Action/Equal Opportunity
Employer.

Questions may be directed to Dr. Glass or Dr. Earl Godfrey
(godfreew-at-borg.evms.edu).

Please inform qualified individuals of this opportunity. Thank you very much.


Earl W. Godfrey, Ph.D.
Dept. of Pathology and Anatomy
Eastern Virginia Medical School
P.O. Box 1980
Norfolk, VA 23501

street address: 700 W. Olney Rd.
Lewis Hall Rm. 3077a
Norfolk, VA 23507

(757) 446-5609
FAX (757) 446-5719
Email: godfreew-at-borg.evms.edu





From daemon Mon Jun 4 17:59:01 2001



From: davidbock-at-mindspring.com ()
Date: Mon, 4 Jun 2001 17:49:29 -0500
Subject: Ask-A-Microscopist

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Below is the result of your feedback form. It was submitted by
(davidbock-at-mindspring.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, June
3, 2001 at 20:06:47
---------------------------------------------------------------------------

Email: davidbock-at-mindspring.com
Name: David B.

Organization: --

Education: Graduate College

Location: LA, CA

Question: I would like to create a very large image of human blood at
~4000 times magnification. Ideally, it would be a single image that I
would print at 10 feet x 30 feet. I imagine each red blood cell
would be about 3" across in the final printed version.

Is it possible to take such a picture? Would I have to take a series
of shots and then join them with Photoshop? What about resolution?

Thanks for your help with this,
David

---------------------------------------------------------------------------


From daemon Mon Jun 4 17:59:01 2001



From: bubidabub-at-worldnet.att.net (by way of Nestor J. Zaluzec)
Date: Mon, 4 Jun 2001 17:28:40 -0500
Subject: Ask-A-Microscopist

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Below is the result of your feedback form. It was submitted by
(bubidabub-at-worldnet.att.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, June
4, 2001 at 01:18:52
---------------------------------------------------------------------------

Email: bubidabub-at-worldnet.att.net
Name: Robin Olsen

Organization: Northampton Community College

Education: Undergraduate College

Location: Easton, PA Northampton County

Question: Could you please tell me what type of microorganisms one
can expect to find in environmental dust taken from outdoor
playground equipment? I am a college student taking microbiology at
NCC and I am having a difficult time finding resources for research
regarding organisms in dust and dirt. If you could tell me some
things I could expect to culture from a sample taken from a
playground, or tell me some resources where I could find this
information I would greatly appreciate it! Thanks!!

---------------------------------------------------------------------------


From daemon Mon Jun 4 17:59:01 2001



From: bubidabub-at-worldnet.att.net ()
Date: Mon, 4 Jun 2001 17:49:16 -0500
Subject: Ask-A-Microscopist

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Below is the result of your feedback form. It was submitted by
(bubidabub-at-worldnet.att.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, June
4, 2001 at 01:18:52
---------------------------------------------------------------------------

Email: bubidabub-at-worldnet.att.net
Name: Robin Olsen

Organization: Northampton Community College

Education: Undergraduate College

Location: Easton, PA Northampton County

Question: Could you please tell me what type of microorganisms one
can expect to find in environmental dust taken from outdoor
playground equipment? I am a college student taking microbiology at
NCC and I am having a difficult time finding resources for research
regarding organisms in dust and dirt. If you could tell me some
things I could expect to culture from a sample taken from a
playground, or tell me some resources where I could find this
information I would greatly appreciate it! Thanks!!

---------------------------------------------------------------------------


From daemon Tue Jun 5 03:15:01 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 5 Jun 2001 09:12:33 +0100 (GMT Daylight Time)
Subject: Re: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
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HI Karen,

I have an old, but brilliant, book 'Leybold Vacuum
Handbook' by K. Diels and R. Jaeckel (Translated by H. Adam
and J. Edwards) Pergamon Press, 1966. It contains
explainations of these problems, descriptions of
experiments to measure outgassing and desorption, and rates
for most elements and compounds that may be used in vacuum
systems.

If you want a simple way to test the various materials find
a pumping rig and pump it down to it's ultimate pressure at
least overnight, ensure that the ultimate pressure is of
the same order as your microscope. Then vent it to dry
nitrogen and pump down again noting the pressure change
with time to it's ultimate pressure (say for 1 hour). Then
vent it and introduce the unknown component and pump down
again noting the time and pressure. The change in pumping
speed (rate of decrease in pressure) will be due to the
outgassing. No fancy units but a direct comparison for
your problem. Remember that to make a real comparison of
outgassing rates you need to have similar amounts of
material in similar forms, ie. same surface areas and
volumes. However, I guess you are more concerned with the
comparison between different mounting techniques so similar
specimens mounted by the various techniques would be OK.

To save you a lot of time in experimenting I suggest that
you use the minimum amount of mounting material, you want
the smallest possible surface area to see the vacuum and
you want the lowest desorption (outgassing) rate and lowest
vapour pressure.

Of your 4 options I would not use sticky pads or waxes. I
would only use epoxy or conductive inks and pastes fully
underneath the specimen and ensure that the small amount
used was fully cured and dried by pumping it out in a
vacuum rig before loading it into the microscope. I would
also store it under vacuum before loading.

Good luck,
Ron

}
} We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:
}
} 1. sticky dots
} 2. conductive inks and pastes
} 3. epoxies
} 4. waxes
}
} Is there an accepted method for rating these materials?
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Jun 5 08:09:11 2001



From: kellyburns-at-cfl.rr.com ()
Date: Tue, 5 Jun 2001 08:03:32 -0500
Subject: Ask-A-Microscopist: When preparing a smear....

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Below is the result of your feedback form. It was submitted by
(kellyburns-at-cfl.rr.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html#form on Monday,
June 4, 2001 at 22:29:41
---------------------------------------------------------------------------

Email: kellyburns-at-cfl.rr.com
Name: Kelly Burns

Organization: Valencia Community College

Education: Undergraduate College

Location: Ocoee, FL, US

Question: When preparing a smear, why is it important to
use tap water versus distilled water?

Thank You :)

---------------------------------------------------------------------------


From daemon Tue Jun 5 09:00:40 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 05 Jun 2001 09:53:00 -0500
Subject: SEM mounting materials

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Karen Dye wrote the following:
==========================================================
We just acquired a new high resolution SEM (FEG) and want to keep it "clean"
. How can I find out about out-gassing rates and the relative "cleanliness"
of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?
==========================================================
We at SPI Supplies have been dealing with this particular issue for more
than thirty years. I don't think your question has the kind of absolute
answer you might have been seeking, for the following reasons:

a) There are at least several different sources of "sticky dots", and they
do not all behave the same. Some off-gas more than others and some are more
sensitive to the beam than others. Also other factors such as age and
storage conditions can effect the behavior of some of these adhesive
materials in vacuum.

b) The conductive inks and pastes similarly represent different
compositions, they are not all the same, and do not all come from the same
place, but in addition to that, the "off-gassing characteristics" also
depend on how thick of a layer has been applied and whether there is a
"skin" that forms on the top, resulting in a lingering "wet" area underneath
the skin. This effect can be even more problematic for a paste. The
details of the formulation can influence the tendency of the composition to
form such a skin, which acts as a barrier to diffusion.

c) The typical epoxy can be cured using more or less hardener, and it can
be cured at room temperature or at higher temperatures. A room temperature
cure with less rather than more hardener increases the chances for there
being more unpolymerized material being present to off-gas and cause
problems. The use of more hardener and some residence time at a higher
temperature tends to minimize the off-gassing effect.

d) Waxes tend to be nonconductive and beam sensitive. Some have
plasticizers that migrate to the surface and off-gas. We try to avoid waxes
as a mounting medium for this kind of application in any kind of SEM. I
have waxes like dental waxes in mind in the making of that comment.


We have adopted our own "test" for UHV compatibility. Simply put, it is a
measure of the deterioration of vacuum when a test specimen is inserted into
a UHV system. We found that the SPI double sided conductive carbon sheet
material, when a 10 mm square was used, was inserted into the UHV system,
there was no visible "meter deflection" on the LED display for the vacuum.
Since the adhesive is the same as what is used in the SPI carbon tape and
double sided discs, we would expect the same result would be found for them
as well. We know that some similar appearing materials offered by others
will perform as well on such a test. But not in all instances. We know
this might not be the perfect test, but over the years it has been quite
effective in terms of validating those materials that are better for this
kind of application than others. Obviously, the worst performing material,
if just a tiny bit is used, could end up "passing" the test, just as an
inordinately large piece of the SPI double sided conductive sheets could
flunk on such a test. So it is important to standardize on the area to be
exposed to the UHV conditions if one is doing such a comparative test.

Having said all of that, we believe that the "dry adhesives" are far better
than wet paints. But we also believe that the best laboratory practice
would involve the use of the smallest amount of adhesive possible, and for
that reason, we usually recommend the use of the sheets, which are cut out
to the size desired for each sample, rather than the discs for which the
smallest is still probably bigger than what is needed for many samples. Of
course my other factors, such as personal preference and experience come
into play so I am sure there could be selection based on factors other than
purely that of off-gassing.

Disclaimer: SPI Supplies offers all four types of mountants for use for the
mounting of SEM samples.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Tue Jun 5 09:27:17 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 5 Jun 2001 10:21:44 -0400
Subject: Imaging Information URL

Contents Retrieved from Microscopy Listserver Archives
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I provide a copy of the following URL to a site with rich detail on imaging
for those of us in the mood to learn.

http://www.tasi.ac.uk/welcome.html

This site is TASI: "Technical Advisory Service for Images". Suggest
further, to find tree of info, click on "FRAMEWORK"

Regards to all,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Tue Jun 5 11:09:18 2001



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Tue, 05 Jun 2001 11:53:30 -0400
Subject: sectioning tantalum matrix

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I am posting this for a college, Lauri Wyner, in the Pathology Core.
I have no idea how to section this stuff...!

Thanks!
Maria Ericsson

My question is:
I have a carbon coated porous tantalum matrix approximately 1 cm in
circumference and 2 mm thick. This matrix has fixed cells adhered to the
surface and throughout. I would like to embed this, make slides and stain
for H&E to confirm the presences of cells as well as immunohistochemistry
to characterize them. I am looking for suggestions on how I can section
this matrix while maintaining its overall structure. Any information would
be greatly appreciated.



Lauri Wyner
DF/HCC Central Pathology Cores Coordinator
Harvard Medical School
G1-126, Goldenson Building
220 Longwood Avenue
Boston, MA 02115
Tele: (617) 432-4947
Fas: (617) 432-6474
Lauri_wyner-at-hms.harvard.edu



From daemon Tue Jun 5 12:28:28 2001



From: Rajesh Patel :      rpatel-at-umdnj.edu
Date: Tue, 5 Jun 2001 13:27:32 -0400
Subject: salary survey

Contents Retrieved from Microscopy Listserver Archives
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I beleive that some time a go a salray survey was
done for electron microscopy field. How and where
can I access that info.


Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu


From daemon Tue Jun 5 13:04:38 2001



From: Gilbert, Charles :      Charles.Gilbert-at-carolinashealthcare.org
Date: Tue, 5 Jun 2001 13:59:58 -0400
Subject: Delete - Disregard; this is a test; Outlook97 annoyances.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a test for being able to send from our institution to the MSA List
server. MS Outlook97 is annoying in being somewhat inscrutable for sending
html unless you know how to find it's hidden secrets. This we have done. :
)
-------------------------------------
Name: Charles Gilbert VOC: (704) 355-0604
Carolinas Medical Center FAX: (704) 355-8424
Dept of Pediatric Research digPager: (704) 355-4088 : 2058
PO Box 32861
Charlotte, NC 28232-2861


DISCLAIMER: {"The opinions are my own and not necessarily shared by my
employer."}

Sent by Outlook under the 60 Hz electron recycling project
-------------------------------------
"I know of no safe depository of the ultimate powers of the society but the
people themselves; and if we think them not enlightened enough to exercise
their control with wholesome discretion, the remedy is not to take it from
them, but to inform their discretion by education." (Thomas Jefferson;
Letter to Wm. C. Jarvis, 1820)




***********************************************************************
This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you.


From daemon Tue Jun 5 13:37:23 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 05 Jun 2001 09:53:00 -0500
Subject: SEM mounting materials

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Karen Dye wrote the following:
==========================================================
We just acquired a new high resolution SEM (FEG) and want to keep it "clean"
How can I find out about out-gassing rates and the relative "cleanliness"
of various sample preparation supplies:

1. sticky dots
2. conductive inks and pastes
3. epoxies
4. waxes

Is there an accepted method for rating these materials?
==========================================================
We at SPI Supplies have been dealing with this particular issue for more
than thirty years. I don't think your question has the kind of absolute
answer you might have been seeking, for the following reasons:

a) There are at least several different sources of "sticky dots", and they
do not all behave the same. Some off-gas more than others and some are more
sensitive to the beam than others. Also other factors such as age and
storage conditions can effect the behavior of some of these adhesive
materials in vacuum.

b) The conductive inks and pastes similarly represent different
compositions, they are not all the same, and do not all come from the same
place, but in addition to that, the "off-gassing characteristics" also
depend on how thick of a layer has been applied and whether there is a
"skin" that forms on the top, resulting in a lingering "wet" area underneath
the skin. This effect can be even more problematic for a paste. The
details of the formulation can influence the tendency of the composition to
form such a skin, which acts as a barrier to diffusion.

c) The typical epoxy can be cured using more or less hardener, and it can
be cured at room temperature or at higher temperatures. A room temperature
cure with less rather than more hardener increases the chances for there
being more unpolymerized material being present to off-gas and cause
problems. The use of more hardener and some residence time at a higher
temperature tends to minimize the off-gassing effect.

d) Waxes tend to be nonconductive and beam sensitive. Some have
plasticizers that migrate to the surface and off-gas. We try to avoid waxes
as a mounting medium for this kind of application in any kind of SEM. I
have waxes like dental waxes in mind in the making of that comment.


We have adopted our own "test" for UHV compatibility. Simply put, it is a
measure of the deterioration of vacuum when a test specimen is inserted into
a UHV system. We found that the SPI double sided conductive carbon sheet
material, when a 10 mm square was used, was inserted into the UHV system,
there was no visible "meter deflection" on the LED display for the vacuum.
Since the adhesive is the same as what is used in the SPI carbon tape and
double sided discs, we would expect the same result would be found for them
as well. We know that some similar appearing materials offered by others
will perform as well on such a test. But not in all instances. We know
this might not be the perfect test, but over the years it has been quite
effective in terms of validating those materials that are better for this
kind of application than others. Obviously, the worst performing material,
if just a tiny bit is used, could end up "passing" the test, just as an
inordinately large piece of the SPI double sided conductive sheets could
flunk on such a test. So it is important to standardize on the area to be
exposed to the UHV conditions if one is doing such a comparative test.

Having said all of that, we believe that the "dry adhesives" are far better
than wet paints. But we also believe that the best laboratory practice
would involve the use of the smallest amount of adhesive possible, and for
that reason, we usually recommend the use of the sheets, which are cut out
to the size desired for each sample, rather than the discs for which the
smallest is still probably bigger than what is needed for many samples. Of
course my other factors, such as personal preference and experience come
into play so I am sure there could be selection based on factors other than
purely that of off-gassing.

Disclaimer: SPI Supplies offers all four types of mountants for use for the
mounting of SEM samples.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656

e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================







From daemon Tue Jun 5 14:05:45 2001



From: Wiggins, Winston :      Winston.Wiggins-at-carolinashealthcare.org
Date: Tue, 5 Jun 2001 15:01:21 -0400
Subject: Particle size

Contents Retrieved from Microscopy Listserver Archives
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Listers,
Can anyone refer me to a company or lab that can do sub-micron particle size
and distribution studies, especially but not necessarily, in the southeast
US? It involves a study injecting small wear debris particles into a
skeletal joint.
Thanks for any help.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor June 4, 2001 2:44 PM
Cannon Electron Microscopy Lab Ofc: 704-355-1267
Carolinas Medical Center Lab: 704-355-7220
P.O. Box 32861 (Ship to: 1000 Blythe Blvd ) Fax:
704-355-0589
Charlotte, NC 28232-2861 (Ship to: 28203 )
Winston.Wiggins-at-CarolinasHealthCare.org {mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



***********************************************************************
This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you.


From daemon Tue Jun 5 14:27:01 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 5 Jun 2001 15:25:49 -0400
Subject: RE: Outgassing, etc.

Contents Retrieved from Microscopy Listserver Archives
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The general question of outgassing and its effect on the performance
of vacuum systems is discussed in some detail in Section 2.9 of my
book, 'Vacuum Methods in Electron Microscopy' (see:
http://www.2spi.com/catalog/books/book48.html and
http://pup.princeton.edu/titles/6484.html for details).

There is one interesting way in which unexpectedly large amounts of
solvents and gases can get introduced into an SEM when carbon or
silver paint is used to mount a specimen. This can occur if the paint
is smeared on a mounting stub in such a way that the entire area
underneath the specimen is covered, and then only a relatively short
time is allowed for the paint to dry. Then only the paint around the
edges of the specimen will dry, while that underneath the specimen
can remain wet. When inserted into the SEM the solvent from the wet
paint underneath the specimen can slowly diffuse outward through the
'dry' paint around the edges, thereby providing a strong source of
outgassing for a long time.

If you really must use a paing to mount specimens, I think it is best
to only put a few small dabs of it at several spots around the
periphery of the specimen. Then there will be a better opportunity
for the solvent to evaporate out from each small dab, and if
sufficient time is allowed for the drying process, no wet paint will
be left trapped underneath the specimen.

Better yet, make some simple clips or clamps that will hold the
specimen in place mechanically, without the use of solvents or
adhesives. With a little ingenuity and some pliers you can fashion a
clip to hold almost any specimen from a piece of heavy copper wire or
a paperclip.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Tue Jun 5 14:43:36 2001



From: David Wilbur :      dwilbu01-at-emerald.tufts.edu
Date: Tue, 05 Jun 2001 15:37:58 -0400
Subject: SEM/EDS Need port cover for JEOL 840 microscope

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My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
and unfortunately, I cannot locate the original cover for the EDS port
on the microscope. Neither Oxford not JEOL can supply a cover. I do
not want to lose the use of the SEM for imaging while the detector is
repaired. The microscope is a JEOL JXA-840. As far as I know, the
column is identical to the JSM-840. Is there anyone out there able and
willing to help me with a sale, rent, or loan of a suitable cover until
my x-ray detector is repaired.

Thank you.

Dave Wilbur

--
__________________________________
David J. Wilbur, Ph.D.
Instrumentation Specialist
Department of Chemistry
Tufts University
voice: 617-627-2163
Fax: 617-627-3443
email: dwilbu01-at-tufts.edu
__________________________________




From daemon Tue Jun 5 15:00:29 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 05 Jun 2001 15:00:12 -0500
Subject: Re: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
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I can agree with Ron about the waxes. I've used a JEOL 6600F and Hitachi S-4700s
and neither of these instruments likes wax. The wax will outgas so badly that the
loadlocks won't pump down, so you never get it into the main chamber.
Superglue-type glues work good (fully cured) and I've had no trouble with black carbon
double-sided tape. I also use carbon (graphite) paint after a 15-minute bake at about 40-50
degrees C. A vacuum dryer could also be used if your specimen was heat-sensitive.
I don't much care for silver paint; it takes too long to dry and is hard to remove if necessary.
For the ultimate in cleanliness, remember to handle your mounts, stages, etc. (anything that goes
into the main chamber) with gloves. This will prevent finger oils from being dispersed in the system.

Ron Doole wrote:
----------- {snip} --------------

} To save you a lot of time in experimenting I suggest that
} you use the minimum amount of mounting material, you want
} the smallest possible surface area to see the vacuum and
} you want the lowest desorption (outgassing) rate and lowest
} vapour pressure.
}
} Of your 4 options I would not use sticky pads or waxes. I
} would only use epoxy or conductive inks and pastes fully
} underneath the specimen and ensure that the small amount
} used was fully cured and dried by pumping it out in a
} vacuum rig before loading it into the microscope. I would
} also store it under vacuum before loading.
}
} Good luck,
} Ron
}
} }
} } We just acquired a new high resolution SEM (FEG) and want to keep it "clean". How can I find out about out-gassing rates and the relative "cleanliness" of various sample preparation supplies:
} }
} } 1. sticky dots
} } 2. conductive inks and pastes
} } 3. epoxies
} } 4. waxes
} }
} } Is there an accepted method for rating these materials?
} }
} }
}
} ----------------------
} Mr. R.C. Doole
} Department of Materials,
} University of Oxford.
} Parks Road, Oxford. OX1 3PH. UK.
} Phone +44 (0) 1865 273701
} Fax +44 (0) 1865 283333
} ron.doole-at-materials.ox.ac.uk

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
DSPS Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Tue Jun 5 15:42:09 2001



From: OCONNELL-at-ltu.edu
Date: Tue, 05 Jun 2001 16:39:24 -0400 (EDT)
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position available in an independent testing lab in a suburb of Detroit, MI.
Knowledge of ISI SS 40 required. Materials testing experience a plus.
For information contact 734-668-3309 and leave message.




From daemon Tue Jun 5 15:51:28 2001



From: Frida.Maiers-at-co.hennepin.mn.us
Date: Tue, 5 Jun 2001 15:46:47 -0500
Subject: Safety

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Several questions about safety.. What do you use for gloves when handling
both resins and fixatives? What procedures are out there for precipitating
lead? Is anyone aware of any suspected instances of EM chemical exposure
(resins, osmium), documented cases and/or references about such? Our
institution would appreciate any information to help them answer questions
about this unique part of the lab.



From daemon Tue Jun 5 18:07:56 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Wed, 6 Jun 2001 09:02:20 +1000
Subject: RE: Out-gassing, etc.

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Rons advice is all very sound, but you have overlooked the effect of
temperature. Even a modest rise in temperature increases vapour pressure and
therefore outgassing markedly. Unless a cold-trap is used the objective area is
rather warmer than room temperature. To cure samples so that later outgassing
is lessened, store specimens at a suitably increased temperature and under a
modest vacuum.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, June 05, 2001 6:13 PM, Ron Doole
[SMTP:ron.doole-at-materials.oxford.ac.uk] wrote:
}
}
} HI Karen,
}
} I have an old, but brilliant, book 'Leybold Vacuum
} Handbook' by K. Diels and R. Jaeckel (Translated by H. Adam
} and J. Edwards) Pergamon Press, 1966. It contains
} explainations of these problems, descriptions of
} experiments to measure outgassing and desorption, and rates
} for most elements and compounds that may be used in vacuum
} systems.
}
} If you want a simple way to test the various materials find
} a pumping rig and pump it down to it's ultimate pressure at
} least overnight, ensure that the ultimate pressure is of
} the same order as your microscope. Then vent it to dry
} nitrogen and pump down again noting the pressure change
} with time to it's ultimate pressure (say for 1 hour). Then
} vent it and introduce the unknown component and pump down
} again noting the time and pressure. The change in pumping
} speed (rate of decrease in pressure) will be due to the
} outgassing. No fancy units but a direct comparison for
} your problem. Remember that to make a real comparison of
} outgassing rates you need to have similar amounts of
} material in similar forms, ie. same surface areas and
} volumes. However, I guess you are more concerned with the
} comparison between different mounting techniques so similar
} specimens mounted by the various techniques would be OK.
}
} To save you a lot of time in experimenting I suggest that
} you use the minimum amount of mounting material, you want
} the smallest possible surface area to see the vacuum and
} you want the lowest desorption (outgassing) rate and lowest
} vapour pressure.
}
} Of your 4 options I would not use sticky pads or waxes. I
} would only use epoxy or conductive inks and pastes fully
} underneath the specimen and ensure that the small amount
} used was fully cured and dried by pumping it out in a
} vacuum rig before loading it into the microscope. I would
} also store it under vacuum before loading.
}
} Good luck,
} Ron
}
} }
} } We just acquired a new high resolution SEM (FEG) and want to keep it
} } "clean". How can I find out about out-gassing rates and the relative
} } "cleanliness" of various sample preparation supplies:
} }
} } 1. sticky dots
} } 2. conductive inks and pastes
} } 3. epoxies
} } 4. waxes
} }
} } Is there an accepted method for rating these materials?
} }
} }
}
} ----------------------
} Mr. R.C. Doole
} Department of Materials,
} University of Oxford.
} Parks Road, Oxford. OX1 3PH. UK.
} Phone +44 (0) 1865 273701
} Fax +44 (0) 1865 283333
} ron.doole-at-materials.ox.ac.uk



From daemon Wed Jun 6 06:53:10 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 06 Jun 2001 07:49:13 -0400
Subject: Re: SEM/EDS Need port cover for JEOL 840 microscope

Contents Retrieved from Microscopy Listserver Archives
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Dave,
Unless you have been running an exceptionally clean and good vacuum, a
rubber stopper of the correct size will do nicely as a temporary
substitute. In particular, make sure that it's not too small so that it
doesn't get sucked through the port at 15 psi. Otherwise, after a
couple of hours of pumping, the outgassing should be minimal (better
after overnight).

Clean the sides of the stopper with IPA or some similar solvent and use
a very small amount of your favorite vacuum grease around the sides.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

David Wilbur wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
} and unfortunately, I cannot locate the original cover for the EDS port
} on the microscope. Neither Oxford not JEOL can supply a cover. I do
} not want to lose the use of the SEM for imaging while the detector is
} repaired. The microscope is a JEOL JXA-840. As far as I know, the
} column is identical to the JSM-840. Is there anyone out there able and
} willing to help me with a sale, rent, or loan of a suitable cover until
} my x-ray detector is repaired.
}
} Thank you.
}
} Dave Wilbur
}
} --
} __________________________________
} David J. Wilbur, Ph.D.
} Instrumentation Specialist
} Department of Chemistry
} Tufts University
} voice: 617-627-2163
} Fax: 617-627-3443
} email: dwilbu01-at-tufts.edu {mailto:dwilbu01-at-tufts.edu}
} __________________________________
}
}
}
}
}




From daemon Wed Jun 6 08:29:08 2001



From: HARRISm-at-esm-semi.co.uk
Date: Wed, 06 Jun 2001 14:19 +0000 (GMT)
Subject: Filament life but not as we know it .

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Dear Colleagues ,

We currently use either Denka or Fei filaments in our
FESEM which have generally given similar results over similar
lifetimes .
However for various reasons we are mow experiencing occasional stray
magnetic field interference with corresponding deterioration in image
quality .
I have never really thought it through but what's actually happening
at the tip ?..

1. Apart from occasional irritating beam 'sway' is physical damage
being done to the tungsten tip with this interference and

2. Assuming a fluid zirconia film/ball exists at the tip of the
tungsten filament is this being consumed more quickly influencing
lifetime ?

Best regards

Martyn


From M Harris
Device Engineering
ESM Ltd , Cardiff Rd .
Newport , South Wales
NP10 8YJ .

Email harrism-at-esm-semi.co.uk




From daemon Wed Jun 6 08:54:46 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Wed, 6 Jun 2001 09:46:20 -0400
Subject: salary survey

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A comprehensive microscopy salary survey was published in the old EMSA
Bulletin in the early 80's. It is too old to be useful. More recently,
there was a smaller survey in "Microscopy Today." Check with Don Grimes
at: microtoday-at-mindspring.com

Perhaps it's time for a new comprehensive survey.



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



"Rajesh Patel" {rpatel-at-umdnj.edu} on 06/05/2001 01:27:32 PM

To: {microscopy-at-sparc5.microscopy.com}
cc:


I beleive that some time a go a salray survey was
done for electron microscopy field. How and where
can I access that info.


Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu






From daemon Wed Jun 6 08:55:44 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 6 Jun 2001 09:52:20 -0400
Subject: RE: SEM/EDS Need port cover for JEOL 840 microscope

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Dear Dave,
While the exact fit would be nice, the dimensions of the requirement
would be nicer. Also, if you haven't called the JEOL service center near
you, you might try that. I have parts galore, but I have to know more about
the item.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: David Wilbur
} Sent: Tuesday, June 5, 2001 3:37 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM/EDS Need port cover for JEOL 840 microscope
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
} and unfortunately, I cannot locate the original cover for the EDS port
} on the microscope. Neither Oxford not JEOL can supply a cover. I do
} not want to lose the use of the SEM for imaging while the detector is
} repaired. The microscope is a JEOL JXA-840. As far as I know, the
} column is identical to the JSM-840. Is there anyone out there able and
} willing to help me with a sale, rent, or loan of a suitable cover until
} my x-ray detector is repaired.
}
} Thank you.
}
} Dave Wilbur
}
} --
} __________________________________
} David J. Wilbur, Ph.D.
} Instrumentation Specialist
} Department of Chemistry
} Tufts University
} voice: 617-627-2163
} Fax: 617-627-3443
} email: dwilbu01-at-tufts.edu
} __________________________________
}
}
}
}


From daemon Wed Jun 6 10:10:32 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 6 Jun 2001 11:05:08 -0400
Subject: RE: sectioning tantalum matrix

Contents Retrieved from Microscopy Listserver Archives
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I would suggest glycol methacrylate embedment (as a start) and thin
sectioning with a carbide knife (expensive, but well worth the cost when
materials are 'hard')

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Maria Ericsson
} Sent: Tuesday, June 5, 2001 11:53 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: sectioning tantalum matrix
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am posting this for a college, Lauri Wyner, in the Pathology Core.
} I have no idea how to section this stuff...!
}
} Thanks!
} Maria Ericsson
}
} My question is:
} I have a carbon coated porous tantalum matrix approximately 1 cm in
} circumference and 2 mm thick. This matrix has fixed cells adhered to the
} surface and throughout. I would like to embed this, make slides and stain
} for H&E to confirm the presences of cells as well as immunohistochemistry
} to characterize them. I am looking for suggestions on how I can section
} this matrix while maintaining its overall structure. Any information would
}
} be greatly appreciated.
}
}
}
} Lauri Wyner
} DF/HCC Central Pathology Cores Coordinator
} Harvard Medical School
} G1-126, Goldenson Building
} 220 Longwood Avenue
} Boston, MA 02115
} Tele: (617) 432-4947
} Fas: (617) 432-6474
} Lauri_wyner-at-hms.harvard.edu
}
}
}


From daemon Wed Jun 6 10:11:29 2001



From: Ingram, Mike :      MIngram-at-rodel.com
Date: Wed, 6 Jun 2001 11:06:15 -0400
Subject: EDS and High Magnification

Contents Retrieved from Microscopy Listserver Archives
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I keep running into users who believe that High magnification for an SEM,
say 20,000X and greater is a benefit to EDS analysis. I have always been
under the impression that high magnification is not a benefit to EDS due
to the large interaction volume of X-rays. Comments please.


Mike Ingram
Rodel Inc.


From daemon Wed Jun 6 10:31:08 2001



From: JHoffpa464-at-aol.com
Date: Wed, 6 Jun 2001 11:26:48 EDT
Subject: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


this is a test


From daemon Wed Jun 6 10:44:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 6 Jun 2001 11:38:22 -0400
Subject: EDS and High Magnification

Contents Retrieved from Microscopy Listserver Archives
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The keyword here is analysis. You are absolutely correct for this. The interaction volume will definitely be much larger than the particular feature that the beam can fully resolve. I preached this for many years and still do to a certain extent. However, it was shown to me by a colleague here after we had been arguing about it that in fact you can get meaningful qualitative information from areas much smaller than the interaction volume. He showed me that putting the beam on a surface feature on a thin film on glass that had a lateral spatial dimension less than 0.1 um and comparing it to an area adjacent to this area gave a significant difference in the spectra. Enough of a difference that it enabled him to pinpoint the problem. We do quite a lot of qualitative analysis and very little quantitative analysis. From that standpoint, higher magnifications can be quite useful, but not for quantitative analysis.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ingram, Mike [mailto:MIngram-at-rodel.com]
Sent: Wednesday, June 06, 2001 11:06 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


I keep running into users who believe that High magnification for an SEM,
say 20,000X and greater is a benefit to EDS analysis. I have always been
under the impression that high magnification is not a benefit to EDS due
to the large interaction volume of X-rays. Comments please.


Mike Ingram
Rodel Inc.


From daemon Wed Jun 6 11:03:21 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Monday, June 04, 2001 11:44 AM
Subject: Out-gassing, etc.

Contents Retrieved from Microscopy Listserver Archives
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Karen and all,

I agree that good vacuum practice is the first line of defense in keeping
your new FEG SEM clean. The use of most of these materials may dirty your
microscope.

XEI Scientific does offer a system to remove outgassed hydrocarbons from the
walls and atmosphere of your microscope. The details about our EVACTRON
SEM-CLEAN system may be found at www.SEMCLEAN.com This system was
specifically developed for keeping FEG SEMs clean.

Ron Vane
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
(650)-369-0133

-----Original Message-----
} From: Karen Dye {karen.dye-at-medtronic.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed Jun 6 11:08:32 2001



From: Bob Roberts :      bobrobs-at-earthlink.net
Date: Wed, 06 Jun 2001 09:07:53 -0700
Subject: Re: SEM/EDS Need port cover for JEOL 840 microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
} and unfortunately, I cannot locate the original cover for the EDS port
} on the microscope. Neither Oxford not JEOL can supply a cover. I do
} not want to lose the use of the SEM for imaging while the detector is
} repaired. The microscope is a JEOL JXA-840. As far as I know, the
} column is identical to the JSM-840. Is there anyone out there able and
} willing to help me with a sale, rent, or loan of a suitable cover until
} my x-ray detector is repaired.
}
David,

There are actually at least two different ports that could
be used for mounting of the xray detector or, in this case,
the blanking plate for the removed detector.

Is it the high take off angle port or the side mount,
circular port?

P.S. I would advise against using a rubber stopper. This
technique could leave you needing more than a blanking plate.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
(480) 967-3946
"www.emlabservices.com"



From daemon Wed Jun 6 12:27:06 2001



From: David Knecht :      knecht-at-uconn.edu
Date: Wed, 6 Jun 2001 13:03:52 -0400
Subject: sutter lambda DG4

Contents Retrieved from Microscopy Listserver Archives
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I would like to hear from users out there about experience with the
Sutter Lambda DG4 fluorescence source/filter changer. It looks very
nice on paper. How is brightness/field uniformity/flexibility for
general use. We want ratio capability which it appears good at, but
we also want versatility for normal single fluor imaging at various
wavelengths. Any comments about using this as opposed to a standard
mercury, standard xenon, or monochromator for a general fluorescence
microscopy facility are appreciated. Thanks- Dave
--

************************************************************
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269-3125
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************


From daemon Wed Jun 6 17:38:26 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 7 Jun 2001 12:44:00 GMT+1200
Subject: rubber stopper ok

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Generally, nothing is happening to the tip by the magnetic fields as the
only components that affect filament life are: pressure, overdriving, & poor
filament construction.

Earl

----- Original Message -----
} From: {"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, June 06, 2001 7:19 AM



A rubber stopper, well-greased with appropriate grease, worked fine
on my 840 in a similar situation, no degradation of vacuum.
Just make absolutely sure that it's big enough that there's no chance
of its being sucked right in, and that nobody knocks into it!

cheers

rtch





}
}
} } My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
} } and unfortunately, I cannot locate the original cover for the EDS port
} } on the microscope. Neither Oxford not JEOL can supply a cover. I do
} } not want to lose the use of the SEM for imaging while the detector is
} } repaired. The microscope is a JEOL JXA-840. As far as I know, the
} } column is identical to the JSM-840. Is there anyone out there able and
} } willing to help me with a sale, rent, or loan of a suitable cover until
} } my x-ray detector is repaired.
} }
} David,
}
} There are actually at least two different ports that could
} be used for mounting of the xray detector or, in this case,
} the blanking plate for the removed detector.
}
} Is it the high take off angle port or the side mount,
} circular port?
}
} P.S. I would advise against using a rubber stopper. This
} technique could leave you needing more than a blanking plate.
Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Thu Jun 7 00:02:33 2001



From: Smartech :      smartech-at-javanet.com
Date: Thu, 7 Jun 2001 01:07:34 -0400
Subject: EDS and High Magnification

Contents Retrieved from Microscopy Listserver Archives
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I recently performed an x-ray map and line scan on a cross sectioned Ag on
Cu sample at 20,000X (15kV) and was able to show little to no migration of
the Ag into the Cu. I was very surprised at how sharp the demarcations
between the layers were at 20,000 in the x-ray maps and line scans. I would
be happy to share the data w/ any interested parties. It is my opinion that
high magnification EDS analysis can be useful. Of' course one can lower the
kV or work with very thin samples to push the limits.

Ric

-----Original Message-----
} From: Ingram, Mike [mailto:MIngram-at-rodel.com]
Sent: Wednesday, June 06, 2001 11:06 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


I keep running into users who believe that High magnification for an SEM,
say 20,000X and greater is a benefit to EDS analysis. I have always been
under the impression that high magnification is not a benefit to EDS due
to the large interaction volume of X-rays. Comments please.


Mike Ingram
Rodel Inc.





From daemon Thu Jun 7 02:26:30 2001



From: erica.vasquez-at-verizon.net
Date: Thu, 7 Jun 2001 01:07:34 -0400
Subject: EDS and High Magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Thu Jun 7 03:05:53 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 7 Jun 2001 03:03:17 -0500
Subject: RE: Filament life but not as we know it .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMF does not generally affect SEMs in the electron column, sufficient
shielding being provided by the normal design of these areas. Once the
beam leaves the final pole piece and enters the sample chamber, though, it
is a different matter. You can tell if the major influence is in the
chamber by doing a simple qualitative measure of the interference at
different working distances. If the problem is greater at the longer
working distance, then the EMF is influencing the beam during its traverse
through the sample chamber.

The same is basically true for vibrational effects. Within the column,
physical movement of the instrument laterally is somewhat cancelled by the
changing proximity to the applied fields of the electron optic system.
Once free of those fields, though, movement of the instrument (and thus
the sample) change the apparent position of the beam. Once again, the
effect is reduced with a reduction in the working distance.

The first step has to be to determine whether the problem is EMF or
vibration - quite possibly both. Since many vibrational sources are linked
to the electrical line frequency, this can sometimes be tough. However,
most modern SEMs should be quite capable of damping 60Hz. vibrations, but
if EMF of any low frequency can come in then all low frequencies will be
allowed in - EMF shielding is far less specific than vibrational.

This leads to the usual trick of trying to determine the offensive
frequency by counting the number of 'saw-tooth' edges in a portion of a
slow image sweep. If the frequency is close to 60Hz then it is probably
EMF.

If the appearance of a problem like this is rather sudden, then the natural
tendency is to find a temporal link to some change in the system. Taking
this approach, though, you have to be sure to include all possible changes
that may have taken place. For both problems, you have to consider if any
changes have been made in the environment around the SEM. Has any
equipment been added or moved in the area that may affect the instrument?
Can the effect be due to a seasonal change in the HVAC equipment in use?
Have there been any increases in the current load on any power
distribution lines near the instrument?

More intimate causes also have to be accessed. Have any electrical cables,
air, water or vacuum lines on the SEM been repositioned? EMF isolation
generally involves putting a distance between any sources and any
receptors. Vibrational isolation generally involves that as well as
several levels of damping that require isolation between them. A simple
cable or tube that is moved and bridges the isolation of one or more stages
can destroy their effectiveness. Moving a computer monitor or external
vacuum gauge controller a few inches can make profound changes in received
EMF.

Finally, one has to consider time. Time, along with other influences, can
produce changes such as corrosion of electrical connections that can cause
high resistance in ground connections (in this case a 'high' resistance of
only a few ohms can cause ground loop currents in instruments that can
become a real problem). Another problem with aging instruments is in the
filter capacitors of their power supplies. Capacitors age, and in doing
so, their values often change. If a circuit is very tightly designed, this
change can result in an increase in a 60 Hz ripple seen in power supplies.
If circuit designs rely heavily on bypass capacitors to reduce ripple to
individual circuits from power supply circuits, a failure of one or more of
those capacitors can also result in increased supply ripple to circuits.
Such failures will have the appearance of an induced EMF effect, but
without the dependence on working distance.

Seems like I've said a lot, but I haven't even scratched the surface.
SEMs, and particularly FESEMs are extremely sensitive instruments. Their
design, implementation and continued operation are a continual balance of
many factors. However, the problems you describe are probably not related
to the use of third-party emitters.

You didn't mention the make and model of the instrument you are using, or
the particular orientation of the emitters you are using. Could you
elaborate on these?

On Wednesday, June 06, 2001 9:19 AM,
"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
[SMTP:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear Colleagues ,
}
} We currently use either Denka or Fei filaments in
our
} FESEM which have generally given similar results over similar
} lifetimes .
} However for various reasons we are mow experiencing occasional stray
} magnetic field interference with corresponding deterioration in
image
} quality .
} I have never really thought it through but what's actually happening
} at the tip ?..
}
} 1. Apart from occasional irritating beam 'sway' is physical damage
} being done to the tungsten tip with this interference and
}
} 2. Assuming a fluid zirconia film/ball exists at the tip of the
} tungsten filament is this being consumed more quickly influencing
} lifetime ?
}
} Best regards
}
} Martyn
}
}
} From M Harris
} Device Engineering
} ESM Ltd , Cardiff Rd .
} Newport , South Wales
} NP10 8YJ .
}
} Email harrism-at-esm-semi.co.uk
}
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Jun 7 03:21:01 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 7 Jun 2001 10:21:40 +0200
Subject: Trends in Nanotechnology 2001 Registration Deadline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
The TNT (Trends in Nanotechnology) 2001 registration deadline at the special
price of 425 Euros is approaching on June 15th. After this date, the
registration fee will be 500 Euros.
The conference is taking place in the first week of September this year in
Segovia, Spain, bringing together the top names in Nanotechnology for a
week's intensive brainstorming. The line up of keynote speakers includes
representatives from NASA, IMEC, Texas Instruments, Sony & Samsung, as well
as universities and research institutes in Europe, the US, & Japan.

Conference topics include

* The Road to High-Volume Nanotechnology Devices
* Nanoelectronics
* Magnetic nanostructures
* Nanomechanical Systems
* Carbon nanotubes and related materials
* Carbon Based Nanoarchitectonics"
* Molecular Nanostructures
* Atomic/Molecular Manipulation
* Nanolithography
* Photonic Crystals
* Nanowires

Full details can be found at http://www.cmp-cientifica.com/tnt2001
Regards
Tim

*****************************************************************
Tim E. Harper CEO
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/




From daemon Thu Jun 7 03:56:22 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 7 Jun 2001 03:52:58 -0500
Subject: RE: EDS and High Magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Interesting question. I generally let users know that the interactions of
electrons and x-ray generation and flourescence limit the analytical volume
to a sphere of 50 - 100 microns in diameter. However, that assumes that
you want to quantify that specific volume. There are many times when you
want different criteria. For example, if one were to do a line scan across
the interface of two differing materials. In this case, we can
artificially determine the boundary as a function of the constantly varying
intensities of their different components even though the compositional
change takes place in an area much smaller than we can reasonable measure.

Such measurement, however, has to be made with the caviat of the
experimental conditions and the 'thresholds' used for determination.
Ideally, these are determined using similar matrix standards - i.e.,
similar materials whose dimensional composition can be verified by other
means.

Thin film or small particle determinations are made using assumptions that
are probably quite accurate, but have to be taken with consideration of the
experimental conditions and their effects on known compositions. One has
to be careful to ensure that the assumptions made by a particular routine
are applicable to their particular circumstances.

Case in point - you currently have the beam located on the exact center of
the interface of two widely different materials. In this case, you would
expect an equal contribution from both materials. However, you have to
consider many factors of x-ray generation by incident electron radiation,
the flourescence and cross-flourescence of the individual materials as well
as the absorption of each for varying wavelengths. Now add into the mix
the morpholgy of your specific samples. We don't currently have all the
answers to these complex problems and rely, instead, on empirical data.
The closer the empirical data to the materials you're looking at, the
better the analysis.

In brief, very minor differences between extremely close materials is
possible, but what you can make of those differences depends on the effort
you put into it.

On Wednesday, June 06, 2001 10:06 AM, Ingram, Mike [SMTP:MIngram-at-rodel.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I keep running into users who believe that High magnification for an SEM,
} say 20,000X and greater is a benefit to EDS analysis. I have always been
} under the impression that high magnification is not a benefit to EDS due
} to the large interaction volume of X-rays. Comments please.
}
}
} Mike Ingram
} Rodel Inc.
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Thu Jun 7 05:30:15 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 7 Jun 2001 20:25:23 +1000
Subject: RE: sectioning tantalum matrix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tantalum is very hard and tough, so I doubt that it will section with either
diamond or tungsten carbide knives. Preparing the material like a geological
section by grinding is a possibility, but not a good one. Chances are that the
grinding material would fill the voids and the tissue would be ground away
first (although ProSciTech and others supply diamond grit embedded in plastic
disks, which are much better in that regard)
Proper vacuum infiltration with a low viscosity resin, cutting with a diamond
blade, grinding with a diamond disc would be my preparation trial.
After that either stain the surface and try your luck with a high power
reflection (metallurgical) microscope, or more likely digest the plastic and
view the specimen in SEM.
If the tantalum could be fractured open, an ESEM my offer some "insights".
There is no simple solution to the preparation of very hard and very soft
materials in one specimen.
Cheers
Jim Darley
Disclaimer: ProSciTech supplies both diamond and TC knives. I advised against
the use of either in this particular case.
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, June 06, 2001 1:54 AM, Maria Ericsson
[SMTP:maria_ericsson-at-hms.harvard.edu] wrote:
}
}
}
} I am posting this for a college, Lauri Wyner, in the Pathology Core.
} I have no idea how to section this stuff...!
}
} Thanks!
} Maria Ericsson
}
} My question is:
} I have a carbon coated porous tantalum matrix approximately 1 cm in
} circumference and 2 mm thick. This matrix has fixed cells adhered to the
} surface and throughout. I would like to embed this, make slides and stain
} for H&E to confirm the presences of cells as well as immunohistochemistry
} to characterize them. I am looking for suggestions on how I can section
} this matrix while maintaining its overall structure. Any information would
} be greatly appreciated.
}
}
}
} Lauri Wyner
} DF/HCC Central Pathology Cores Coordinator
} Harvard Medical School
} G1-126, Goldenson Building
} 220 Longwood Avenue
} Boston, MA 02115
} Tele: (617) 432-4947
} Fas: (617) 432-6474
} Lauri_wyner-at-hms.harvard.edu
}
}



From daemon Thu Jun 7 07:09:09 2001



From: JHoffpa464-at-aol.com
Date: Thu, 7 Jun 2001 08:03:25 EDT
Subject: EM COSTING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


we are in the process of cost our EM service, that is a cost breakdown for
doing EM. i know the actual costs are very small. i was wondering if anyone
has done it recently. we are a diagnostic lab.
john hoffpauir
cooper hospital


From daemon Thu Jun 7 07:15:11 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 7 Jun 2001 08:14:15 -0400
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just out of curiousity. . . how much X-radiation penetrates the rubber
stopper?

Marie


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Thu Jun 7 08:57:51 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 7 Jun 2001 23:51:08 +1000
Subject: RE: rubber stopper ok

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I vote with Ritchie. Rubber stoppers are great for temporary problems,
particularly for finding vacuum leaks. One stopper can eliminate a more complex
assembly. I somehow prefer the silicone rubber stoppers.
However, do consider X-ray penetration. Particularly on instruments above
20kV.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, June 07, 2001 10:44 PM, Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz]
wrote:

}
}
} A rubber stopper, well-greased with appropriate grease, worked fine
} on my 840 in a similar situation, no degradation of vacuum.
} Just make absolutely sure that it's big enough that there's no chance
} of its being sucked right in, and that nobody knocks into it!
}
} cheers
}
} rtch
}
}
}
}
}
} }
} }
} } } My Oxford EDS x-ray detector needs to be returned to Oxford for repair,
} } } and unfortunately, I cannot locate the original cover for the EDS port
} } } on the microscope. Neither Oxford not JEOL can supply a cover. I do
} } } not want to lose the use of the SEM for imaging while the detector is
} } } repaired. The microscope is a JEOL JXA-840. As far as I know, the
} } } column is identical to the JSM-840. Is there anyone out there able and
} } } willing to help me with a sale, rent, or loan of a suitable cover until
} } } my x-ray detector is repaired.
} } }
} } David,
} }
} } There are actually at least two different ports that could
} } be used for mounting of the xray detector or, in this case,
} } the blanking plate for the removed detector.
} }
} } Is it the high take off angle port or the side mount,
} } circular port?
} }
} } P.S. I would advise against using a rubber stopper. This
} } technique could leave you needing more than a blanking plate.
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand


From daemon Thu Jun 7 11:02:58 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 07 Jun 2001 17:25:54 -0500
Subject: Sectioning Ta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In order to re-send this email, I had to change the subject line to stop a rejection because of the r word.

-----Original Message-----
} From: Beauregard, Paul A.
Sent: Thursday, June 07, 2001 11:39 AM
To: Microscopy-at-sparc5.microscopy.com


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:

====================================================
Tantalum is very hard and tough, so I doubt that it will section with either

diamond or tungsten carbide knives. Preparing the material like a geological

section by grinding is a possibility, but not a good one. Chances are that
the
grinding material would fill the voids and the tissue would be ground away
first (although ProSciTech and others supply diamond grit embedded in
plastic
disks, which are much better in that regard)
Proper vacuum infiltration with a low viscosity resin, cutting with a
diamond
blade, grinding with a diamond disc would be my preparation trial.
After that either stain the surface and try your luck with a high power
reflection (metallurgical) microscope, or more likely digest the plastic and

view the specimen in SEM.
If the tantalum could be fractured open, an ESEM my offer some "insights".
There is no simple solution to the preparation of very hard and very soft
materials in one specimen.
=====================================================
Actually Ta **can** be thin sectioned if it is done by someone with the
proper experience and who is using the right kind of diamond knife. We have
offered our SPI #02888-AB sectioned Ta foil on a grid for AEM studies for
some number of years; see URL
http://www.2spi.com/catalog/standards/aem.html

The Ta system that prompted the original posting was thicker, however, it is
also porous, and if properly infiltrated with resin, and assuming the
porosity is above some point, in principle, at least, there is no reason why
it could not be thin sectioned for TEM. No method is really artifact free,
but some artifacts are more easy to recognize than others. Knife induced
artifacts are anisotripic (e.g. directional) in nature and can be more
easily recognized (as artifacts) than artifacts caused by say, ion milling,
which are isotropic in nature.

When I say "right kind of diamond knife", I am not suggesting that the SPI
diamond knife could do something above and beyond what other diamond knives
could do. What I am saying is that there is a process of selection of the
optimum knife angle, because one might have to be prepared to use a knife
with a fairly low (for materials science work) knife angle, rather than a
more blunt angle, but with the downside is that it will wear out more
quickly. That might make for great business for a diamond knife supplier,
but it does tend to get expensive for the user who is not so experienced
with this kind of sectioning.

Disclaimer: SPI Supplies offers diamond knives, both materials and life
science, and we have done this kind of sectioning, as a service, for
commercial clients for over thirty years.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Jun 7 19:44:28 2001



From: zaluzec-at-microscopy.com
Date: Thu, 7 Jun 2001 19:40:56 -0500
Subject: Administrivia: Listserver Rejection Errors...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

I hate to say this, but alot of mail got rejected today by accident.

I was updating the junk mail filters last night and unintentionally created
a new filter which rejected the majority of the mail posted today.

If you received a rejection message indicating that you had a subject line of
\b$ please repost your message. It was not your fault, but mine.


Sorry....

Nestor
Your Friendly Neighborhood SysOp


From daemon Thu Jun 7 19:50:44 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Thu, 07 Jun 2001 17:48:21 -0700
Subject: sectioning tantalum matrix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following up on Jim's comments about using mechanical cutting and
grinding
techniques, I have some insight that may help. Jim mentioned that
"There is no
simple solution to the preparation of very hard and very soft materials
in one
specimen". This is true to some degree, but there are solutions. For
example,
the use of a wire saw is actually ideal for cutting through materials
containing
both hard and soft phases. The wire saw can be used either with an
abrasive
slurry or with a diamond impregnated wire. Use with an abrasive slurry
is ideal
for cutting materials of various hardness without damage or the smearing
effect
that you would see with a diamond wheel saw.

To be honest, I have no idea if this saw would do what you want it to do
in this
application, however if the difficulty is the hard/soft combination, the
wire saw
is one potentially viable solution. Also, I'm not sure if you need to
embed the
sample, but I would suggest forgoing tat step, if possible, if oyu are
going to
try the wire saw technique.

DISCLAIMER: South Bay Technology pioneered the development of the wire
saw in the
early 60s and continues to produce wire saws for many applications
today.

I hope this helps.

David

Jim at Proscitech wrote:

}
------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
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}
-----------------------------------------------------------------------.

}
} Tantalum is very hard and tough, so I doubt that it will section with
either
} diamond or tungsten carbide knives. Preparing the material like a
geological
} section by grinding is a possibility, but not a good one. Chances are
that the
} grinding material would fill the voids and the tissue would be ground
away
} first (although ProSciTech and others supply diamond grit embedded in
plastic
} disks, which are much better in that regard)
} Proper vacuum infiltration with a low viscosity resin, cutting with a
diamond
} blade, grinding with a diamond disc would be my preparation trial.
} After that either stain the surface and try your luck with a high
power
} reflection (metallurgical) microscope, or more likely digest the
plastic and
} view the specimen in SEM.
} If the tantalum could be fractured open, an ESEM my offer some
"insights".
} There is no simple solution to the preparation of very hard and very
soft
} materials in one specimen.
} Cheers
} Jim Darley
} Disclaimer: ProSciTech supplies both diamond and TC knives. I advised
against
} the use of either in this particular case.
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, June 06, 2001 1:54 AM, Maria Ericsson
} [SMTP:maria_ericsson-at-hms.harvard.edu] wrote:
} }
} }
} }
} } I am posting this for a college, Lauri Wyner, in the Pathology Core.

} } I have no idea how to section this stuff...!
} }
} } Thanks!
} } Maria Ericsson
} }
} } My question is:
} } I have a carbon coated porous tantalum matrix approximately 1 cm in
} } circumference and 2 mm thick. This matrix has fixed cells adhered to
the
} } surface and throughout. I would like to embed this, make slides and
stain
} } for H&E to confirm the presences of cells as well as
immunohistochemistry
} } to characterize them. I am looking for suggestions on how I can
section
} } this matrix while maintaining its overall structure. Any information
would
} } be greatly appreciated.
} }
} }
} }
} } Lauri Wyner
} } DF/HCC Central Pathology Cores Coordinator
} } Harvard Medical School
} } G1-126, Goldenson Building
} } 220 Longwood Avenue
} } Boston, MA 02115
} } Tele: (617) 432-4947
} } Fas: (617) 432-6474
} } Lauri_wyner-at-hms.harvard.edu
} }
} }

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Thu Jun 7 21:34:53 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Jun 2001 21:28:05 -0500
Subject: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

While infiltrating a set of specimens (insect abdomens and thoraxes) in LR
White, something occurred that has so far defied my attempts to figure out.
I was doing
side by side microwave and conventional fixations, dehydrations, and
embeddings. The microwave specimens were dehydrated in acetone, behaved
normally, and we
have polymerized blocks. However I dehydrated the conventional set in an
ethanol series, as we have done many times before in LR White. The first
infiltration step
was 2 parts ethanol to one part LR White Medium Grade at room temp, and
upon going later to change into 1:1, I found that the resin/ETOH mix had
partially
polymerized into a spongy, granular mass. Uh-oh, bad resin, I said, along
with a few other choice words.

After the initial panic, I ended up putting the chunks into pure LR White
from a newly opened bottle and the partially polymerized resin seemed to go
back into solution. I
ran them through a couple more changes of pure resin, then left them on a
rocker overnight at room temperature. Checking them this morning, I found
two of the
samples were fine, and the third had polymerized into a rubbery mass.
Same bottle of resin, same identically processed samples, same everything.

An additional tube with a sample of the "bad" resin was also happily
unpolymerized, as was the remainder of the "bad" resin in the bottle, which
I had left in the fume
hood overnight at room temperature. The "bad" resin was slightly more
than a year old and has been refrigerated at 4 C since we got it. The
second resin is about 10
months old and has also been constantly refrigerated. Neither had any
accelerator in them (at least none added by us).

I have my share of problems with LR White, but this one really has us
puzzled. Could there have been something in the samples that triggered a
polymerization? Can
LR White react with some plastics in this way? (We were using a
relatively new batch of Eppendorf tubes that seem more hydrophobic than our
previous ones.) Could
it have been the full moon?

Regards,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu
http://biotech.missouri.edu/emc



From daemon Thu Jun 7 21:34:54 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 7 Jun 2001 16:29:12 -1000 (HST)
Subject: TEM - effects of osmolarity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

Does anyone have a reference for or some transmission electron micrographs
that illustrate the effects of osmolarity of buffers on animal cells? I
would like to show students on tour of our facility shrinkage and swelling
of cells and organelles. I have other wonderful artifacts to show,
including one of my first sections with chatter so bad it looks like
mini-blinds...

Mahalo!
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Jun 7 23:58:25 2001



From: Arthur Day :      ard-at-ansto.gov.au
Date: Fri, 8 Jun 2001 14:53:41 +1000
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Doesn't anybody supply X-ray proof rubber bungs for microscope ports?

No worries. Just wrap a few sheets of lead foil around the column and
make sure pregnant operators stay below 5kV ;-(

Surely something somewhat less desperate could be cobbled together
out of metal without too much extra work?



From daemon Fri Jun 8 03:34:29 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 8 Jun 2001 09:28:17 +0100 (GMT Daylight Time)
Subject: Re: TEM - effects of osmolarity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Biomedical Electron Microscopy by Maunsbach A B & Afzelius
B A (1999) has several pages of illustrations of shrinkage
etc.

Dave


On Thu, 7 Jun 2001 16:29:12 -1000 (HST) Tina Carvalho
{tina-at-pbrc.hawaii.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, All-
}
} Does anyone have a reference for or some transmission electron micrographs
} that illustrate the effects of osmolarity of buffers on animal cells? I
} would like to show students on tour of our facility shrinkage and swelling
} of cells and organelles. I have other wonderful artifacts to show,
} including one of my first sections with chatter so bad it looks like
} mini-blinds...
}
} Mahalo!
} Tina
}
} http://www.pbrc.hawaii.edu/bemf/microangela
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Jun 8 07:03:21 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 8 Jun 2001 21:53:48 +1000
Subject: RE: Sectioning Ta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck - the point of the original request was not to section Ta - which is hard
enough, but do show the cells attached to the Ta. If you think that you can
section Ta without the cells being ripped away, then do it. I will praise your
skills when I can see useful results.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, June 08, 2001 8:26 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com]
wrote:
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
}
} ====================================================
} Tantalum is very hard and tough, so I doubt that it will section with either
}
} diamond or tungsten carbide knives. Preparing the material like a geological
}
} section by grinding is a possibility, but not a good one. Chances are that
} the
} grinding material would fill the voids and the tissue would be ground away
} first (although ProSciTech and others supply diamond grit embedded in
} plastic
} disks, which are much better in that regard)
} Proper vacuum infiltration with a low viscosity resin, cutting with a
} diamond
} blade, grinding with a diamond disc would be my preparation trial.
} After that either stain the surface and try your luck with a high power
} reflection (metallurgical) microscope, or more likely digest the plastic and
}
} view the specimen in SEM.
} If the tantalum could be fractured open, an ESEM my offer some "insights".
} There is no simple solution to the preparation of very hard and very soft
} materials in one specimen.
} =====================================================
} Actually Ta **can** be thin sectioned if it is done by someone with the
} proper experience and who is using the right kind of diamond knife. We have
} offered our SPI #02888-AB sectioned Ta foil on a grid for AEM studies for
} some number of years; see URL
} http://www.2spi.com/catalog/standards/aem.html
}
} The Ta system that prompted the original posting was thicker, however, it is
} also porous, and if properly infiltrated with resin, and assuming the
} porosity is above some point, in principle, at least, there is no reason why
} it could not be thin sectioned for TEM. No method is really artifact free,
} but some artifacts are more easy to recognize than others. Knife induced
} artifacts are anisotripic (e.g. directional) in nature and can be more
} easily recognized (as artifacts) than artifacts caused by say, ion milling,
} which are isotropic in nature.
}
} When I say "right kind of diamond knife", I am not suggesting that the SPI
} diamond knife could do something above and beyond what other diamond knives
} could do. What I am saying is that there is a process of selection of the
} optimum knife angle, because one might have to be prepared to use a knife
} with a fairly low (for materials science work) knife angle, rather than a
} more blunt angle, but with the downside is that it will wear out more
} quickly. That might make for great business for a diamond knife supplier,
} but it does tend to get expensive for the user who is not so experienced
} with this kind of sectioning.
}
} Disclaimer: SPI Supplies offers diamond knives, both materials and life
} science, and we have done this kind of sectioning, as a service, for
} commercial clients for over thirty years.
}
} Chuck
}
} PS: Please remember that we are nearly 100% paperless and we would ask that
} any reply to this message be by way of the "reply" feature on your software,
} so that the entire string of correspondence can come back to us and all be
} in one place.
}


From daemon Fri Jun 8 07:46:13 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Jun 2001 08:42:53 -0400
Subject: Re: TEM - effects of osmolarity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} Hi, All-
}
} Does anyone have a reference for or some transmission electron micrographs
} that illustrate the effects of osmolarity of buffers on animal cells? I
} would like to show students on tour of our facility shrinkage and swelling
} of cells and organelles. I have other wonderful artifacts to show,
} including one of my first sections with chatter so bad it looks like
} mini-blinds...
}
} Mahalo!
} Tina

Arborgh et al. J. Ultrastruc. Res. 56:339-350, 1976. A classic study.

}
}
} http://www.pbrc.hawaii.edu/bemf/microangela
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Jun 8 08:01:47 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 8 Jun 2001 22:59:03 +1000
Subject: RE: rubber stopper, suction X-rays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The utility of rubber stoppers to seal a SEM has been doubted.
1 Somebody may chose too smaller stopper size and the stopper may be sucked
into the column. Good point, just like noting that the stopper must be inserted
from the outside of the SEM. Yes!

2 The X-ray objection is one which needs to be addressed. I had looked up that
data some years ago, when in need of temporary blanks.
Because of the questions I again looked for the data, now on the marvelous
Internet . The common Monte Carlo programs would give similar results, but the
show the bulk of the X-ray interaction, whereas we are interested in maximum
penetration. I recommend
http://www-cxro.lbl.gov/optical_constants/atten2.html
This site allows some data entry and then calculates the attenuation length at
which the X-ray intensity falls of to 1eV at the surface. Assuming that Teflon
is close to rubber in absorption, then 20keV X-ray photons are attenuated in
5mm of rubber. Whereas 30keV photons are only attenuated in about 12mm of
rubber. Clearly, it would be unwise to run the kV of a TEM, when blanked with a
rubber stopper.
It is also clear that a SEM/ Probe when operated at 20 kV would not produce
X-rays capable of penetrating a normal rubber stopper.
Incidentally, a 20kV beam would produce somewhat lower KeV X-ray photons. X-ray
generation depends on the interaction with electron shells. Only the heavier
metals can produce powerful X-rays and to reach full fluorescence (maximum
production) the kV needs to be about 2x that of the X-ray KeV.
Cheers
Jim Darley
PS Red stoppers contain Iron oxide pigment, whereas black stoppers (I presume)
have negligible metal content.
Question: Considering that iron has greater X-ray stopping power than carbon,
should we assume that it is smarter to use red stoppers???
The opposing argument would be that the iron could generate more high energy
X-rays.

I don't know the answer (would be amused to learn though), but I prefer
silicone rubber stoppers because they form a better vacuum seal.

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, June 07, 2001 10:14 PM, Marie E. Cantino
[SMTP:cantino-at-uconnvm.uconn.edu] wrote:
}
}
} Just out of curiousity. . . how much X-radiation penetrates the rubber
} stopper?
}
} Marie
}

} I vote with Ritchie. Rubber stoppers are great for temporary problems,
} particularly for finding vacuum leaks. One stopper can eliminate a more
complex
} assembly. I somehow prefer the silicone rubber stoppers.
} However, do consider X-ray penetration. Particularly on instruments above
} 20kV.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com


}
} }
} } A rubber stopper, well-greased with appropriate grease, worked fine
} } on my 840 in a similar situation, no degradation of vacuum.
} } Just make absolutely sure that it's big enough that there's no chance
} } of its being sucked right in, and that nobody knocks into it!
} }
} } cheers
} }
} } rtch
} }
}
} Dr. Marie E. Cantino
} Dept. of Physiology and Neurobiology, U-2131
} University of Connecticut
} Storrs, CT 06269-2131
} Phone: 860-486-3588
} Fax: 860-486-6369
}



From daemon Fri Jun 8 08:05:57 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 08 Jun 2001 10:15:49 -0400
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I usually wrap several layers of aluminum foil over the stopper if I need to
use it.
This will stop most of the X-rays.
Fortunately, the EDS port is to the rear of the column and pointing upward.

Earl



----- Original Message -----
} From: "Arthur Day" {ard-at-ansto.gov.au}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, June 07, 2001 9:53 PM




-------- Original Message --------


Pehaps someone could provide a better analysis, but I have a couple of
anecdotes:

I had a Canadian customer (20+ years ago) who specialized in very long
EDS x-ray sweeps in either dot map form or a combined YZ modulated
form. As I recall, some of these scans were up to 16 hours long. He
had a Plexiglass port cover so that he could easily see his chamber
geometry. This had a metal cover that was velvet lined for light
leakage. Once he put his radiation badge inside the metal cover for a
month, then turned it in. There apparently was nothing out of the
ordinary, as his rad peo;le never said "boo". Perhaps it's a commentary
on the rad people, but a full month exposure?

Second, I had another customer get me some rad figures on an SEM gun
operating at 30kV and 150uA (1.5x10E-4). The bottom line was that the
x-ray output from the gun was very serious, but all the guns are
enclosed in steel. No problem. At the specimen for EDS work we're
usually talking about 200-600pA (2-6x10E-10), or roughly 6 orders of
magnitude less current and the stopper is going to absorb some of the
x-rays generated. Is this really a problem?

I'm not talking about TEMs, as they can be very dangerous. The current
at the screen is much higher, the excitation voltage is much higher and
there is this large expanse of glass that MUST be leaded. Perhaps the
currents used for WDS would also present a problem (10-100 nA or 10E-8
to 10E-7) at 2 to 3 orders of magnitude more than EDS.

Don't forget, those nice color displays on the four computers
surrounding you in your lab also generate x-rays and their beam currents
are in the mA range (10E-3) and an accelerating voltage of about 25kV.
There may be a decelerating grid and leaded glass on the front, but I
think you will find the the shielding on the rear of the monitor is not
so rigorous, Your dentist's x-rays are about 70kV and only one order of
magnitude greater current.

I'd love some feed-back from someone who knows radiation because I've
felt that applying TEM rules to SEMs is gross over-kill, especially with
so many color monitors around. How often do you have your decelerating
grid checked for proper operation? If it doesn't operate correctly,
your exposure could be very dangerous, given the time that one sits in
front of these things and their distance from your face.

If I'm way off base, I'd like to know and know why.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Arthur Day wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Just out of curiousity. . . how much X-radiation penetrates the rubber
} } stopper?
} }
} } Marie
} }
}
} Doesn't anybody supply X-ray proof rubber bungs for microscope ports?
}
} No worries. Just wrap a few sheets of lead foil around the column and
} make sure pregnant operators stay below 5kV ;-(
}
} Surely something somewhat less desperate could be cobbled together out
} of metal without too much extra work?
}
}
}
}



From daemon Fri Jun 8 09:19:20 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 07 Jun 2001 17:25:54 -0500
Subject: Sectioning Ta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:

====================================================
Tantalum is very hard and tough, so I doubt that it will section with either

diamond or tungsten carbide knives. Preparing the material like a geological

section by grinding is a possibility, but not a good one. Chances are that
the
grinding material would fill the voids and the tissue would be ground away
first (although ProSciTech and others supply diamond grit embedded in
plastic
disks, which are much better in that regard)
Proper vacuum infiltration with a low viscosity resin, cutting with a
diamond
blade, grinding with a diamond disc would be my preparation trial.
After that either stain the surface and try your luck with a high power
reflection (metallurgical) microscope, or more likely digest the plastic and

view the specimen in SEM.
If the tantalum could be fractured open, an ESEM my offer some "insights".
There is no simple solution to the preparation of very hard and very soft
materials in one specimen.
=====================================================
Actually Ta **can** be thin sectioned if it is done by someone with the
proper experience and who is using the right kind of diamond knife. We have
offered our SPI #02888-AB sectioned Ta foil on a grid for AEM studies for
some number of years; see URL
http://www.2spi.com/catalog/standards/aem.html

The Ta system that prompted the original posting was thicker, however, it is
also porous, and if properly infiltrated with resin, and assuming the
porosity is above some point, in principle, at least, there is no reason why
it could not be thin sectioned for TEM. No method is really artifact free,
but some artifacts are more easy to recognize than others. Knife induced
artifacts are anisotripic (e.g. directional) in nature and can be more
easily recognized (as artifacts) than artifacts caused by say, ion milling,
which are isotropic in nature.

When I say "right kind of diamond knife", I am not suggesting that the SPI
diamond knife could do something above and beyond what other diamond knives
could do. What I am saying is that there is a process of selection of the
optimum knife angle, because one might have to be prepared to use a knife
with a fairly low (for materials science work) knife angle, rather than a
more blunt angle, but with the downside is that it will wear out more
quickly. That might make for great business for a diamond knife supplier,
but it does tend to get expensive for the user who is not so experienced
with this kind of sectioning.

Disclaimer: SPI Supplies offers diamond knives, both materials and life
science, and we have done this kind of sectioning, as a service, for
commercial clients for over thirty years.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From daemon Fri Jun 8 09:29:04 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 8 Jun 2001 09:24:18 -0500
Subject: Re: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy: This has happened a bunch of times to me. It only happens
with osmicated tissues. I have run up the same tisse +/- osmication
and premature polymerization only occurs in the osmicated ones - not
every time or at the same rate. I guess there is something that
doesn't get rinsed out that can trigger it - perhaps more so when the
LRW is aging. I am starting a LRW infiltration with osmicated tissue
using a brand new bottle of LRW and I will let you know what happens.
my tissues were in glass vials so it isn't the plastic tubes. it has
happened at both 4 C and room temp. i have a vague recollection
posting this on the microscopy listserver and not getting much of a
response. maddening problem, isn't it?

} ------------------------------------------------------------.
}
}
} Dear Listers,
}
} While infiltrating a set of specimens (insect abdomens and thoraxes) in LR
} White, something occurred that has so far defied my attempts to figure out.
} I was doing
} side by side microwave and conventional fixations, dehydrations, and
} embeddings. The microwave specimens were dehydrated in acetone, behaved
} normally, and we
} have polymerized blocks. However I dehydrated the conventional set in an
} ethanol series, as we have done many times before in LR White. The first
} infiltration step
} was 2 parts ethanol to one part LR White Medium Grade at room temp, and
} upon going later to change into 1:1, I found that the resin/ETOH mix had
} partially
} polymerized into a spongy, granular mass. Uh-oh, bad resin, I said, along
} with a few other choice words.
}
} After the initial panic, I ended up putting the chunks into pure LR White
} from a newly opened bottle and the partially polymerized resin seemed to go
} back into solution. I
} ran them through a couple more changes of pure resin, then left them on a
} rocker overnight at room temperature. Checking them this morning, I found
} two of the
} samples were fine, and the third had polymerized into a rubbery mass.
} Same bottle of resin, same identically processed samples, same everything.
}
} An additional tube with a sample of the "bad" resin was also happily
} unpolymerized, as was the remainder of the "bad" resin in the bottle, which
} I had left in the fume
} hood overnight at room temperature. The "bad" resin was slightly more
} than a year old and has been refrigerated at 4 C since we got it. The
} second resin is about 10
} months old and has also been constantly refrigerated. Neither had any
} accelerator in them (at least none added by us).
}
} I have my share of problems with LR White, but this one really has us
} puzzled. Could there have been something in the samples that triggered a
} polymerization? Can
} LR White react with some plastics in this way? (We were using a
} relatively new batch of Eppendorf tubes that seem more hydrophobic than our
} previous ones.) Could
} it have been the full moon?
}
} Regards,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} (573) 882-8304
} (573) 884-5414 (fax)
} email: tindallr-at-missouri.edu
} http://biotech.missouri.edu/emc
}

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Jun 8 09:45:02 2001



From: zaluzec-at-sparc5.microscopy.com
Date: Thu, 7 Jun 2001 19:40:56 -0500
Subject: Administrivia: Listserver Rejection Errors...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

You might have nailed it. These were osmicated tissues, since they weren't
intended for immuno. Our client prefers LR White for making thick sections
for light microscopy, so these specimens were prepared with osmium and UA
post-fixations. Given the large size and difficult nature of insect parts,
it's very possible that something remained in the tissue despite several
lengthy washes. At least we have back-up specimens!

Thanks for the response. At least it's something to work with.

Randy

-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, June 08, 2001 9:24 AM
To: Tindall, Randy D.
Cc: Microscopy-at-msa.microscopy.com


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues....

I hate to say this, but alot of mail got rejected today by accident.

I was updating the junk mail filters last night and unintentionally created
a new filter which rejected the majority of the mail posted today.

If you received a rejection message indicating that you had a subject line of
\b$ please repost your message. It was not your fault, but mine.


Sorry....

Nestor
Your Friendly Neighborhood SysOp



From daemon Fri Jun 8 09:54:21 2001



From: Eric :      biology-at-ucla.edu
Date: Fri, 08 Jun 2001 07:53:10 -0700
Subject: Little Survey...EM Wise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the wealth of knowledge on the Microscopy list,



I just wanted to conduct a simple survey for the clinical or diagnostic EM
labs that are around...

Was curious to know how many biopsies are processed in the lab on a yearly
basis..

i.e. Renal Bx., Surgical Bx.

Trying to get an idea to see if we are as they say here overworked..

Here in the lab we do

We do approximately 700 cases a year with 2.5 people working in this lab...

How about you?

Eric A. Rosen
UCLA Medical Center
Electron Microsocpy Facility
Department of Pathology and Lab Medicine



From daemon Fri Jun 8 09:55:14 2001



From: Wiggins, Winston :      Winston.Wiggins-at-carolinashealthcare.org
Date: Fri, 8 Jun 2001 10:32:22 -0400
Subject: Prticle Size Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
Thanks to all who responded to my particle size analysis posting recently.
Reasons, including a heavy workload, would not allow us to accept the
project
at this time. We now have several resources from which to choose. I
appreciate
the time and effort from those who offered constructive answers. From the
one who offered questionable musings... contact me off-line and I'll be more

specific about what can be done with those.
Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor June 8, 2001 10:07 AM
Cannon Electron Microscopy Lab Ofc:
704-355-1267
Carolinas Medical Center Lab:
704-355-7220
P.O. Box 32861 (Ship to: 1000 Blythe Blvd ) Fax: 704-355-0589
Charlotte, NC 28232-2861 (Ship to: 28203 )
WWiggins-at-Carolinas.org {mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



***********************************************************************
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From daemon Fri Jun 8 10:07:14 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 8 Jun 2001 11:03:11 -0400
Subject: Old Microscope Catalogs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have in my possession the following:

Leitz, Catalog #52-D20(3/68) "Leitz Fluorescence
Microscopes"
Scopes: SM-M, SM-D, Labolux-D, Ortholux, Lamphouse
#250, Orthomat
Leitz, Catalog #560-D1(11/68) "Leitz Instruments...in Metal,
Plastics, Electronics...."
Scopes: Orthoplan, Metallux, Metallux-ND, Panphot,
Labolux-UB D, MiniLoad, MiniLoad-POL, Linnik, Tolansky, Mirau, Ultropak,
Aristophot, Orthomat, Microtome #1300, MPV
Leitz, Catalog #51.2-D.40(1/66)"Leitz Research Microscopes
Ortholux...."
Scopes: Ortholux, Aristophot, Orthomat,
Accessories: all.
Leitz, Catalog #500-D10(1/74) "Leitz Instruments for the
Clinical Lab"
Scopes: SM-Lux, Dialux, Diavert, Orthomat-W,
Combiphot, Projection, Microtomes, Photometers,
Leitz, Catalog #500-D10(6/77) "Leitz Instruments for the
Laboratory"
Scopes: HM-Lux, Sm-Lux, Dialux, Diavert,
Orthomat-W, Combiphot, Microtomes, Microprojection, Photometers, and
accessories.

I also have a number of old Leitz "Technical Information Bulletins" that
will be listed later.

Hope there is a use.

FCM

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Fri Jun 8 10:16:12 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 8 Jun 2001 11:12:20 -0400
Subject: RE: sectioning tantalum matrix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I concluded from the original information given that the tantalum matrix the
young lady was speaking about was Cytomatrix (http://www.cytomatrix.com).
The matrix is reportedly a tantalum coated graphite core material that was
first use as a support for bone marrow/bone cell regeneration. That's why I
suggested the glycol methacrylate, though you may still be correct about
it's 'hardness'.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Jim at Proscitech
} Reply To: jim-at-proscitech.com
} Sent: Thursday, June 7, 2001 6:25 AM
} To: 'Maria Ericsson'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: sectioning tantalum matrix
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Tantalum is very hard and tough, so I doubt that it will section with
} either
} diamond or tungsten carbide knives. Preparing the material like a
} geological
} section by grinding is a possibility, but not a good one. Chances are that
} the
} grinding material would fill the voids and the tissue would be ground away
}
} first (although ProSciTech and others supply diamond grit embedded in
} plastic
} disks, which are much better in that regard)
} Proper vacuum infiltration with a low viscosity resin, cutting with a
} diamond
} blade, grinding with a diamond disc would be my preparation trial.
} After that either stain the surface and try your luck with a high power
} reflection (metallurgical) microscope, or more likely digest the plastic
} and
} view the specimen in SEM.
} If the tantalum could be fractured open, an ESEM my offer some "insights".
} There is no simple solution to the preparation of very hard and very soft
} materials in one specimen.
} Cheers
} Jim Darley
} Disclaimer: ProSciTech supplies both diamond and TC knives. I advised
} against
} the use of either in this particular case.
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, June 06, 2001 1:54 AM, Maria Ericsson
} [SMTP:maria_ericsson-at-hms.harvard.edu] wrote:
} }
} }
} }
} } I am posting this for a college, Lauri Wyner, in the Pathology Core.
} } I have no idea how to section this stuff...!
} }
} } Thanks!
} } Maria Ericsson
} }
} } My question is:
} } I have a carbon coated porous tantalum matrix approximately 1 cm in
} } circumference and 2 mm thick. This matrix has fixed cells adhered to the
} } surface and throughout. I would like to embed this, make slides and
} stain
} } for H&E to confirm the presences of cells as well as
} immunohistochemistry
} } to characterize them. I am looking for suggestions on how I can section
} } this matrix while maintaining its overall structure. Any information
} would
} } be greatly appreciated.
} }
} }
} }
} } Lauri Wyner
} } DF/HCC Central Pathology Cores Coordinator
} } Harvard Medical School
} } G1-126, Goldenson Building
} } 220 Longwood Avenue
} } Boston, MA 02115
} } Tele: (617) 432-4947
} } Fas: (617) 432-6474
} } Lauri_wyner-at-hms.harvard.edu
} }
} }
}
}
}


From daemon Fri Jun 8 10:27:59 2001



From: Linda Durbin :      Linda.Durbin-at-exaktusa.com
Date: Fri, 8 Jun 2001 10:12:20 -0500
Subject: sectioning tantalum matrix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with David, there are several ways to cut and grind samples with
multiple materials of varying hardnesses. The EXAKT Cutting/Grinding
Systems were developed just for this purpose. One of the major issues in
cutting or grinding these types of materials is the force required to cut
the hard material vs. the force required to cut the softer material. Like
the diamond wire saw a diamond embedded band system designed to section with
a minimum of force can also achieve excellent results. The reduced force
technology (reduction of the active cutting or grinding surface) is not new
but has new applications in material research. And the technology can be
applied to the grinding process as well to achieve flat surfaces at material
interfaces.

Many of these techniques have been developed for LM in the biomaterials
industry where the materials can vary from cobalt chrome implanted in bone
or bone cement to memory metal stents implanted in soft arteries. The
technology works equally well for rubber bonded to metal or solders layered
on ceramics or carbon fibers embedded in unpolymerized resin. One of the
key features of reducing the force and changing the cutting or grinding
direction is also to prevent smearing of one material over an interface.
Biomaterials researchers have been the driving force in developing this
technology for the past 15 years and for LM it is a viable alternative to
standard metallographic preparations.

Disclaimer: EXAKT Technologies, Inc. is the North American Distributor for
EXAKT Apparatebau the manufacturer of various cutting devices and grinders
for multiple material applications.

Linda Durbin

-----Original Message-----
} From: David Henriks [mailto:henriks-at-southbaytech.com]
Sent: Thursday, June 07, 2001 7:48 PM
To: Microscopy Listerver


Following up on Jim's comments about using mechanical cutting and
grinding
techniques, I have some insight that may help. Jim mentioned that
"There is no
simple solution to the preparation of very hard and very soft materials
in one
specimen". This is true to some degree, but there are solutions. For
example,
the use of a wire saw is actually ideal for cutting through materials
containing
both hard and soft phases. The wire saw can be used either with an
abrasive
slurry or with a diamond impregnated wire. Use with an abrasive slurry
is ideal
for cutting materials of various hardness without damage or the smearing
effect
that you would see with a diamond wheel saw.

To be honest, I have no idea if this saw would do what you want it to do
in this
application, however if the difficulty is the hard/soft combination, the
wire saw
is one potentially viable solution. Also, I'm not sure if you need to
embed the
sample, but I would suggest forgoing tat step, if possible, if oyu are
going to
try the wire saw technique.

DISCLAIMER: South Bay Technology pioneered the development of the wire
saw in the
early 60s and continues to produce wire saws for many applications
today.

I hope this helps.

David

Jim at Proscitech wrote:

}
------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.

}
} Tantalum is very hard and tough, so I doubt that it will section with
either
} diamond or tungsten carbide knives. Preparing the material like a
geological
} section by grinding is a possibility, but not a good one. Chances are
that the
} grinding material would fill the voids and the tissue would be ground
away
} first (although ProSciTech and others supply diamond grit embedded in
plastic
} disks, which are much better in that regard)
} Proper vacuum infiltration with a low viscosity resin, cutting with a
diamond
} blade, grinding with a diamond disc would be my preparation trial.
} After that either stain the surface and try your luck with a high
power
} reflection (metallurgical) microscope, or more likely digest the
plastic and
} view the specimen in SEM.
} If the tantalum could be fractured open, an ESEM my offer some
"insights".
} There is no simple solution to the preparation of very hard and very
soft
} materials in one specimen.
} Cheers
} Jim Darley
} Disclaimer: ProSciTech supplies both diamond and TC knives. I advised
against
} the use of either in this particular case.
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, June 06, 2001 1:54 AM, Maria Ericsson
} [SMTP:maria_ericsson-at-hms.harvard.edu] wrote:
} }
} }
} }
} } I am posting this for a college, Lauri Wyner, in the Pathology Core.

} } I have no idea how to section this stuff...!
} }
} } Thanks!
} } Maria Ericsson
} }
} } My question is:
} } I have a carbon coated porous tantalum matrix approximately 1 cm in
} } circumference and 2 mm thick. This matrix has fixed cells adhered to
the
} } surface and throughout. I would like to embed this, make slides and
stain
} } for H&E to confirm the presences of cells as well as
immunohistochemistry
} } to characterize them. I am looking for suggestions on how I can
section
} } this matrix while maintaining its overall structure. Any information
would
} } be greatly appreciated.
} }
} }
} }
} } Lauri Wyner
} } DF/HCC Central Pathology Cores Coordinator
} } Harvard Medical School
} } G1-126, Goldenson Building
} } 220 Longwood Avenue
} } Boston, MA 02115
} } Tele: (617) 432-4947
} } Fas: (617) 432-6474
} } Lauri_wyner-at-hms.harvard.edu
} }
} }

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Fri Jun 8 11:20:19 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Fri, 8 Jun 2001 09:12:21 -0700 (PDT)
Subject: Quantifying actin orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear fellow microscopists,

I have have a task of quantifying the actin distribution in cultured
endothelial cells and subsequently comparing different culture conditions.
So far I have managed to isolate the filaments and threshold them. Now...I
wonder what is the best way to put a number on the distribution within
each cell. One possibility I was tinking of, is using a sobel operator to
give the direction of the lines representing the filaments and then
display the data as histograms of filament orientations.

Has anyone done this or know of a good wayto go about this type of
analysis?

Bob
University of Washington
Seattle



From daemon Fri Jun 8 11:21:01 2001



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Fri, 08 Jun 2001 11:20:25 -0500
Subject: Question about sputter coating using Pt target

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been using an older Balzer's sputter coater to coat SEM samples with
Pt. Lately the grain structure of the Pt coating has become much more
obvious (at around 100,000x.) Any experts have a thought on why this is
occurring and how to improve the process?
Thanks for any guidelines and help.
Regards,
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Fri Jun 8 11:59:40 2001



From: Alan J. Kruger :      kruger-at-email.marc.usda.gov
Date: Fri, 08 Jun 2001 12:05:24 -0500
Subject: LM: Formula for calculating dept of focus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone lead me to a reference or the formula to calculate dept of
focus on light microscopes?

Al Kruger
USDA Meat Animal Research Center



From daemon Fri Jun 8 11:59:41 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 8 Jun 2001 12:55:14 -0400
Subject: RE: EM COSTING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did some looking into costs/hr and finally came to the conclusion that if
I made an assumption that well funded institutions with shared facilities
had set prices for instrument use, it probably reflected what the market -
i.e. the granting agencies - would bear. What I discovered was prices that
varied but stayed in the range of $25 to $35. $29 seemed to be popular,
though I wondered why not the infinitely more popular prince of $29.95.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: "JHoffpa464-at-aol.com"-at-sparc5.microscopy.com
} Sent: Thursday, June 7, 2001 8:03 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: EM COSTING
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} we are in the process of cost our EM service, that is a cost breakdown
} for
} doing EM. i know the actual costs are very small. i was wondering if
} anyone
} has done it recently. we are a diagnostic lab.
} john hoffpauir
} cooper hospital
}
}


From daemon Fri Jun 8 12:17:04 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Friday, June 08, 2001 8:28 AM
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A little more insight on Xray absorption of materials: Twenty years ago when
I was doing EDS XRF, I did a lot of work on Xray filters on the primary
Xray beam from Xray tubes. I designed both edge filters (the absorption
edges of each element can be used to strongly remove X-rays above certain
energies} and "white" filters to remove all Xrays below certain energies. My
white filters were usually layers of filter paper (no Ti pigment). My
experience was that at below 30KV on the Xray tube almost nothing would get
through 1/2
inch of organic materials. Above 30 KeV we had worry about X-ray leakage.
The Xrays tubes (and Electron microscopes) give off both primary lines and
bremsstrahlung xrays. The Bremsstrahlung peak intensity energy in KeV by
rule of thumb is about 2/3 the energy of the electron beam. You may observe
in your SEM by EDS the X-ray energy spectrum. It is that background
spectrum. Since I had a spectrometer I could ready see what energies passed
or started leaking through my filters. You too can do the Xray absorption
spectrum test inside your chamber by placing the stopper or other material
in front of your EDS detector window and see what comes through. The EDS
detector is much more sensitive than a Geiger counter or film.

Therefore, if the SEM is used below 30 kV, then a thick rubber stopper will
absorb the X-rays. Still worried? Stick a radiation badge on the outside of
the stopper to check.

Ronald Vane
XEI Scientific



-----Original Message-----
} From: Ken Converse {qualityimages-at-netrax.net}
To: Arthur Day {ard-at-ansto.gov.au} ; MSA, listserver
{Microscopy-at-sparc5.microscopy.com}





From daemon Fri Jun 8 13:19:33 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 08 Jun 2001 08:42:53 -0400
Subject: Re: TEM - effects of osmolarity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Tina Carvalho wrote:

} Hi, All-
}
} Does anyone have a reference for or some transmission electron micrographs
} that illustrate the effects of osmolarity of buffers on animal cells? I
} would like to show students on tour of our facility shrinkage and swelling
} of cells and organelles. I have other wonderful artifacts to show,
} including one of my first sections with chatter so bad it looks like
} mini-blinds...
}
} Mahalo!
} Tina

Arborgh et al. J. Ultrastruc. Res. 56:339-350, 1976. A classic study.

}
}
} http://www.pbrc.hawaii.edu/bemf/microangela
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Fri Jun 8 14:58:32 2001



From: sghoshro-at-NMSU.Edu
Date: Fri, 8 Jun 2001 13:52:25 -0600 (MDT)
Subject: RE: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

You can try JB-4 embedding media instead of LR White. It works pretty well
for thick sectioning and maitains excellent structure preservation.


Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Fri, 8 Jun 2001, Tindall, Randy D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Tom,
}
} You might have nailed it. These were osmicated tissues, since they weren't
} intended for immuno. Our client prefers LR White for making thick sections
} for light microscopy, so these specimens were prepared with osmium and UA
} post-fixations. Given the large size and difficult nature of insect parts,
} it's very possible that something remained in the tissue despite several
} lengthy washes. At least we have back-up specimens!
}
} Thanks for the response. At least it's something to work with.
}
} Randy
}
} -----Original Message-----
} } From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
} Sent: Friday, June 08, 2001 9:24 AM
} To: Tindall, Randy D.
} Cc: Microscopy-at-msa.microscopy.com
} Subject: Re: TEM: LR White weirdness
}
}
} Randy: This has happened a bunch of times to me. It only happens
} with osmicated tissues. I have run up the same tisse +/- osmication
} and premature polymerization only occurs in the osmicated ones - not
} every time or at the same rate. I guess there is something that
} doesn't get rinsed out that can trigger it - perhaps more so when the
} LRW is aging. I am starting a LRW infiltration with osmicated tissue
} using a brand new bottle of LRW and I will let you know what happens.
} my tissues were in glass vials so it isn't the plastic tubes. it has
} happened at both 4 C and room temp. i have a vague recollection
} posting this on the microscopy listserver and not getting much of a
} response. maddening problem, isn't it?
}
} } ------------------------------------------------------------.
} }
} }
} } Dear Listers,
} }
} } While infiltrating a set of specimens (insect abdomens and thoraxes) in
} LR
} } White, something occurred that has so far defied my attempts to figure out.
} } I was doing
} } side by side microwave and conventional fixations, dehydrations, and
} } embeddings. The microwave specimens were dehydrated in acetone, behaved
} } normally, and we
} } have polymerized blocks. However I dehydrated the conventional set in
} an
} } ethanol series, as we have done many times before in LR White. The first
} } infiltration step
} } was 2 parts ethanol to one part LR White Medium Grade at room temp, and
} } upon going later to change into 1:1, I found that the resin/ETOH mix had
} } partially
} } polymerized into a spongy, granular mass. Uh-oh, bad resin, I said,
} along
} } with a few other choice words.
} }
} } After the initial panic, I ended up putting the chunks into pure LR
} White
} } from a newly opened bottle and the partially polymerized resin seemed to go
} } back into solution. I
} } ran them through a couple more changes of pure resin, then left them on
} a
} } rocker overnight at room temperature. Checking them this morning, I found
} } two of the
} } samples were fine, and the third had polymerized into a rubbery mass.
} } Same bottle of resin, same identically processed samples, same everything.
} }
} } An additional tube with a sample of the "bad" resin was also happily
} } unpolymerized, as was the remainder of the "bad" resin in the bottle, which
} } I had left in the fume
} } hood overnight at room temperature. The "bad" resin was slightly more
} } than a year old and has been refrigerated at 4 C since we got it. The
} } second resin is about 10
} } months old and has also been constantly refrigerated. Neither had any
} } accelerator in them (at least none added by us).
} }
} } I have my share of problems with LR White, but this one really has us
} } puzzled. Could there have been something in the samples that triggered a
} } polymerization? Can
} } LR White react with some plastics in this way? (We were using a
} } relatively new batch of Eppendorf tubes that seem more hydrophobic than our
} } previous ones.) Could
} } it have been the full moon?
} }
} } Regards,
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } (573) 882-8304
} } (573) 884-5414 (fax)
} } email: tindallr-at-missouri.edu
} } http://biotech.missouri.edu/emc
} }
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}



From daemon Fri Jun 8 17:36:05 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 08 Jun 2001 14:13:35 -0700
Subject: RE: EM COSTING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


$29.99 sounds even better

At 12:55 PM 6/8/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Jun 8 17:50:09 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Fri, 8 Jun 2001 15:46:12 -0700
Subject: cryostats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

I am considering acquiring a cryostat for the laboratory. Does anyone have
an experience, positive or negative, with the Bright OTF cryostat? Please
respond to me offline.

Thanks

Steve Barlow




From daemon Fri Jun 8 18:27:31 2001



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Fri, 08 Jun 2001 16:22:49 -0700
Subject: EELS reference spectra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in need of a reference EELS spectum of ZnO (or probably any other
Zn[+2] compound ) to help me interpret some data. I've already checked
the CEMES EELS database to no avail, and I've done some web-searching
with lots of hits but mostly to conference abstracts but no hard data.
I'm attempting to distinguish between Zn metal, for which I have a
reference EELS spectrum, and Zn[+2}.

Tia,
Mike Nesson--
--
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu




From daemon Sat Jun 9 04:46:21 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 9 Jun 2001 04:40:42 -0500
Subject: RE: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken,

You are right in the comparison of SEMs and TEMs. The accelerating
voltages typically used in TEMs creates a much greater concern as do the
glass viewing ports directly open to the electron beam - sample
interactions. In fact, the majority of problems in TEMs have been
inadequate lead content of the glass in those ports. SEMs are inherently
safe from external emissions in that their design results in a generous
metallic shielding surrounding all portions that can generate x-rays.

However, even in SEMs, there can be exceptions. Remember the small
aluminum plug in the columns of ETEC SEMs, the one opposite where the scan
coil cables enter? If that plug is not in place, then there is a potential
for external radiation. While the designs may be inherently safe, there is
still room for error.

Many states require a regular scan for external radiation on any x-ray
generating equipment. While I have heard of quite a few measurable
responses on TEMs (including one just two days ago at a DOE site), I have
never heard of any detectable radiation from an SEM.

Regarding computer monitors, don't worry. While early TV sets did produce
considerable x-rays externally, modern TVs and computer monitors really
don't. Unless you plan on spending 24 hours a day hugging the back side of
a monitor, I wouldn't worry.

Finally, regarding the temporary use of a rubber stopper - It shouldn't
pose a problem. Most of the ports on an SEM, and particularly those for
EDS systems, are on the rear of the chamber. SEMs, given their usual
operating conditions and limitations, are not prodigious x-ray producers.
Most would be absorbed by the thickness of a stopper capable of
withstanding the vacuum pressures, and what little could escape would be
directed away from anyone using the instrument. Since any resulting
radiation would be attenuated by the square of the distance from the source
(in this case the x-rays penetrating the stopper) there would normally be
no detectable exposure except, perhaps, at the surface of the stopper.

I do have a problem with permanent plastic viewports, however. These are
generally placed on the sides of the sample chamber where a more direct
path to the operator is possible. Whenever I find a customer with a
plastic viewport, I always strongly suggest that they keep a suitable cover
over it when they aren't being used to view the sample presentation.
Surprisingly thin ports can be made of materials like polycarbonate that
are capable of withstanding atmospheric pressures.

The real questions should be, if this is a situation that may occur from
time to time, should a proper port cover be fabricated and kept on hand so
that there are no such questions, now or in the future?

On Friday, June 08, 2001 9:16 AM, Ken Converse
[SMTP:qualityimages-at-netrax.net] wrote:
}
} Pehaps someone could provide a better analysis, but I have a couple of
} anecdotes:
}
} I had a Canadian customer (20+ years ago) who specialized in very long
} EDS x-ray sweeps in either dot map form or a combined YZ modulated
} form. As I recall, some of these scans were up to 16 hours long. He
} had a Plexiglass port cover so that he could easily see his chamber
} geometry. This had a metal cover that was velvet lined for light
} leakage. Once he put his radiation badge inside the metal cover for a
} month, then turned it in. There apparently was nothing out of the
} ordinary, as his rad peo;le never said "boo". Perhaps it's a commentary
} on the rad people, but a full month exposure?
}
} Second, I had another customer get me some rad figures on an SEM gun
} operating at 30kV and 150uA (1.5x10E-4). The bottom line was that the
} x-ray output from the gun was very serious, but all the guns are
} enclosed in steel. No problem. At the specimen for EDS work we're
} usually talking about 200-600pA (2-6x10E-10), or roughly 6 orders of
} magnitude less current and the stopper is going to absorb some of the
} x-rays generated. Is this really a problem?
}
} I'm not talking about TEMs, as they can be very dangerous. The current
} at the screen is much higher, the excitation voltage is much higher and
} there is this large expanse of glass that MUST be leaded. Perhaps the
} currents used for WDS would also present a problem (10-100 nA or 10E-8
} to 10E-7) at 2 to 3 orders of magnitude more than EDS.
}
} Don't forget, those nice color displays on the four computers
} surrounding you in your lab also generate x-rays and their beam currents
} are in the mA range (10E-3) and an accelerating voltage of about 25kV.
} There may be a decelerating grid and leaded glass on the front, but I
} think you will find the the shielding on the rear of the monitor is not
} so rigorous, Your dentist's x-rays are about 70kV and only one order of
} magnitude greater current.
}
} I'd love some feed-back from someone who knows radiation because I've
} felt that applying TEM rules to SEMs is gross over-kill, especially with
} so many color monitors around. How often do you have your decelerating
} grid checked for proper operation? If it doesn't operate correctly,
} your exposure could be very dangerous, given the time that one sits in
} front of these things and their distance from your face.
}
} If I'm way off base, I'd like to know and know why.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
} Arthur Day wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
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} }
} } }
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} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
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} } } To Subscribe/Unsubscribe -- Send Email to
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} } } On-Line Help
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} } }
-----------------------------------------------------------------------.
} } }
} } }
} } } Just out of curiousity. . . how much X-radiation penetrates the rubber
} } } stopper?
} } }
} } } Marie
} } }
} }
} } Doesn't anybody supply X-ray proof rubber bungs for microscope ports?
} }
} } No worries. Just wrap a few sheets of lead foil around the column and
} } make sure pregnant operators stay below 5kV ;-(
} }
} } Surely something somewhat less desperate could be cobbled together out
} } of metal without too much extra work?
} }
} }
} }
} }
}
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Sat Jun 9 11:28:56 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 9 Jun 2001 08:32:10 -0700
Subject: Re: Old Microscope Catalogs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I have in my possession the following [Old Microscope Catalogs]:
} I also have a number of old Leitz "Technical Information Bulletins" that
} will be listed later.
} Hope there is a use.
}
Fred -

If you don't get takers for all of these, there's a microscopy book dealer
in England who can probably find them a home:
http://www.savonabooks.free-online.co.uk It would be a pity to just trash
them...

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Jun 9 19:00:52 2001



From: z9bK6i232-at-bizonly.now
Date: Sat, 9 Jun 2001 18:44:36 -0500
Subject: Re: Old Microscope Catalogs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know someone makeing a CDROM of Leitz information that is not copyrighted.
It is going to be a very reasonably priced commerical project.

I would post his name but I am in the middle of changeing computers and I
don't have his email address at hand.

Gordon Couger

----- Original Message -----
} From: "Caroline Schooley" {schooley-at-mcn.org}
To: "Monson, Frederick C." {fmonson-at-wcupa.edu}
Cc: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, June 09, 2001 10:32 AM



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From daemon Sun Jun 10 09:50:41 2001



From: z9bK6i232-at-bizonly.now
Date: Sat, 9 Jun 2001 18:44:36 -0500
Subject: Re: Old Microscope Catalogs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



SEARCH ENGINES DELIVER TARGETED UNIQUE TRAFFIC TO WEB SITE

If you don't find what you're looking for in the first 10 to 40
matches ON A SEARCH ENGINE, what do you do?
If you are like most people, you simply go to the next
search engine and try again.

If your site is not coming up in the TOP 40 matches
On a search engine, you're losing MONEY!

IT 'S THAT SIMPLE!

Improving search engine TRAFFIC means:

MORE HITS
MORE BUSINESS
MORE SUCCESS

The most important advertising dollar
being spent should be for search engine TRAFFIC.

1. Approximately 95% of all Internet users start
with a search engine query.

2. Anyone who comes to your site from a major search
engine is 100 times more likely to become a customer
because they were specifically looking for your product,
goods or services.

3. Search engine traffic gives you substantially more
for your advertising dollar than banners. Moreover
Banners are done on impressions. An impression is
just someone that went to a web page with your
Banner ad on the page. It does not mean your Ad
was seen.

You Can't Get Your Companies Web Site Indexed by the Search Engines?

Unfortunately, this is all too common of a Problem.
You're not the only one frustrated about the length
of time it takes to be listed, or all the pitfalls involved.
It takes anywhere from 2 days to as much as 6 months to be
listed on all the search engines. Waiting several weeks to
months can be extremely frustrating.

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Engines can at any time delay their indexing for maintenance
and many other reasons.

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Lucky if you submit A PAGE just days before an engine does a complete
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Your competitors ARE -at- the mercy of OUR
Marketing Departments.
6 Plus Years Now in the search engine wars,
We are Masters of words like: MP3 - BOOKS
- WEB SITE HOSTING - MARKETING -
- FREE WEB SITES - CASINO -
- CASINO REVIEWS - BALLS -
- LOGOS - ART - ATTORNEY'S
- NEW CAR PARTS - OLD CAR PART -
- NETWORK MARKETING - WATER FILTERS -
- SCALES - AND THE LIST GOES ON - -

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From daemon Sun Jun 10 17:25:13 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Mon, 11 Jun 2001 09:35:07 +1200
Subject: Re: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy and others:

Unwanted polymerisation of LR White resin during infiltration is an
occasional problem with the resin. I have never experienced it with
fresh batches, indeed the only times I have had this problem was
with batches over 1 year old (the recommended use by time). An
possible explanation was suggested to me by Roy Gillett of
London Resin a number of years ago:

Quote
"The reason this pre-polymerisation occurs only with tissue must be
something to do with a tissue constituent catalysing
polymerisation. Older resin is much more susceptibe to this that
fresh monomer becaue of the significant polymer growth that will
inevitably have occurred in the monomer. The most likely
'endogenousd catalyst' from previous experience is likely to be an
amine or peroxide moiety in the tissue"

We have used LR White resin extensively for a long time - perhaps
the reason we don't have a problem is that our resin never gets
anything like a year old before we use it/

Best Wishes

Ian

}
} Dear Listers,
}
} While infiltrating a set of specimens (insect abdomens and thoraxes) in LR
} White, something occurred that has so far defied my attempts to figure out.
} I was doing
} side by side microwave and conventional fixations, dehydrations, and
} embeddings. The microwave specimens were dehydrated in acetone, behaved
} normally, and we
} have polymerized blocks. However I dehydrated the conventional set in an
} ethanol series, as we have done many times before in LR White. The first
} infiltration step
} was 2 parts ethanol to one part LR White Medium Grade at room temp, and
} upon going later to change into 1:1, I found that the resin/ETOH mix had
} partially
} polymerized into a spongy, granular mass. Uh-oh, bad resin, I said, along
} with a few other choice words.
}
} After the initial panic, I ended up putting the chunks into pure LR White
} from a newly opened bottle and the partially polymerized resin seemed to go
} back into solution. I
} ran them through a couple more changes of pure resin, then left them on a
} rocker overnight at room temperature. Checking them this morning, I found
} two of the
} samples were fine, and the third had polymerized into a rubbery mass.
} Same bottle of resin, same identically processed samples, same everything.
}
} An additional tube with a sample of the "bad" resin was also happily
} unpolymerized, as was the remainder of the "bad" resin in the bottle, which
} I had left in the fume
} hood overnight at room temperature. The "bad" resin was slightly more
} than a year old and has been refrigerated at 4 C since we got it. The
} second resin is about 10
} months old and has also been constantly refrigerated. Neither had any
} accelerator in them (at least none added by us).
}
} I have my share of problems with LR White, but this one really has us
} puzzled. Could there have been something in the samples that triggered a
} polymerization? Can
} LR White react with some plastics in this way? (We were using a
} relatively new batch of Eppendorf tubes that seem more hydrophobic than our
} previous ones.) Could
} it have been the full moon?
}
} Regards,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} (573) 882-8304
} (573) 884-5414 (fax)
} email: tindallr-at-missouri.edu
} http://biotech.missouri.edu/emc
}
}



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Mon Jun 11 06:11:45 2001



From: 8IKZ1UJ67-at-yourbox.now
Date: Mon, 11 Jun 2001 09:35:07 +1200
Subject: Re: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon Jun 11 08:25:17 2001



From: Robby Roberson :      robby2-at-asu.edu
Date: Mon, 11 Jun 2001 06:14:39 -0700
Subject: measurments at LM and TEM levels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I have a question about measuring small cellular structures at the light
microscope level using Argus 10 enhancement and measurement software.

I am measuring vesicle diameters in living cells of the fungus Neurospora.
They measure about 350-400 nm in diameter using the Argus 10 software.
With cryofixed TEMs of the same cell type, the only vesicles with the same
distribution as at the LM level measure 150 nm diameter and there are no
structures at 350-400 nm.

All my calibrations of both the LM and TEM have been checked and
double-checked.

It is my understanding that video-enhanced LM can amplify signal (or sizes)
so that structures below limit of LM resolution can be detected (eg,
imaging of individual microtubules with DIC/VELM). I am wondering if this
is the source of this discrepancy that it see and if there are references
that describe this.

Thanks so much,,,,,,
Robby Roberson


***************************************
Robert W. Roberson, PhD
Department of Plant Biology
Molecular and Cellular Biology Program
Arizona State University
Tempe, AZ 85282
Office/Lab 480-965-8618


From daemon Mon Jun 11 09:26:28 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 11 Jun 2001 10:15:19 -0400
Subject: Re: Little Survey...EM Wise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 7:53 AM -0700 6/8/01, Eric wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Jun 11 09:34:28 2001



From: William McManus :      billemac-at-biology.usu.edu
Date: Mon, 11 Jun 2001 08:28:39 -0600
Subject: Re: TEM: LR White weirdness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have notice a strange effect of colored Microcentrifuge tubes. The
colored tubes will cause hardening of the LR White at room temperature.
Something in the coloring dyein the tubes must be acting as a catalyst.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920

-----Original Message-----
} From: IAN HALLETT [mailto:ihallett-at-hortresearch.co.nz]
Sent: Sunday, June 10, 2001 3:35 PM
To: microscopy-at-sparc5.microscopy.com


Randy and others:

Unwanted polymerisation of LR White resin during infiltration is an
occasional problem with the resin. I have never experienced it with
fresh batches, indeed the only times I have had this problem was
with batches over 1 year old (the recommended use by time). An
possible explanation was suggested to me by Roy Gillett of
London Resin a number of years ago:

Quote
"The reason this pre-polymerisation occurs only with tissue must be
something to do with a tissue constituent catalysing
polymerisation. Older resin is much more susceptibe to this that
fresh monomer becaue of the significant polymer growth that will
inevitably have occurred in the monomer. The most likely
'endogenousd catalyst' from previous experience is likely to be an
amine or peroxide moiety in the tissue"

We have used LR White resin extensively for a long time - perhaps
the reason we don't have a problem is that our resin never gets
anything like a year old before we use it/

Best Wishes

Ian

}
} Dear Listers,
}
} While infiltrating a set of specimens (insect abdomens and thoraxes) in
LR
} White, something occurred that has so far defied my attempts to figure
out.
} I was doing
} side by side microwave and conventional fixations, dehydrations, and
} embeddings. The microwave specimens were dehydrated in acetone, behaved
} normally, and we
} have polymerized blocks. However I dehydrated the conventional set in
an
} ethanol series, as we have done many times before in LR White. The first
} infiltration step
} was 2 parts ethanol to one part LR White Medium Grade at room temp, and
} upon going later to change into 1:1, I found that the resin/ETOH mix had
} partially
} polymerized into a spongy, granular mass. Uh-oh, bad resin, I said,
along
} with a few other choice words.
}
} After the initial panic, I ended up putting the chunks into pure LR
White
} from a newly opened bottle and the partially polymerized resin seemed to
go
} back into solution. I
} ran them through a couple more changes of pure resin, then left them on
a
} rocker overnight at room temperature. Checking them this morning, I found
} two of the
} samples were fine, and the third had polymerized into a rubbery mass.
} Same bottle of resin, same identically processed samples, same everything.
}
} An additional tube with a sample of the "bad" resin was also happily
} unpolymerized, as was the remainder of the "bad" resin in the bottle,
which
} I had left in the fume
} hood overnight at room temperature. The "bad" resin was slightly more
} than a year old and has been refrigerated at 4 C since we got it. The
} second resin is about 10
} months old and has also been constantly refrigerated. Neither had any
} accelerator in them (at least none added by us).
}
} I have my share of problems with LR White, but this one really has us
} puzzled. Could there have been something in the samples that triggered a
} polymerization? Can
} LR White react with some plastics in this way? (We were using a
} relatively new batch of Eppendorf tubes that seem more hydrophobic than
our
} previous ones.) Could
} it have been the full moon?
}
} Regards,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} (573) 882-8304
} (573) 884-5414 (fax)
} email: tindallr-at-missouri.edu
} http://biotech.missouri.edu/emc
}
}



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________



From daemon Mon Jun 11 09:48:40 2001



From: Alan Bright :      bright-at-dial.pipex.com
Date: Mon, 11 Jun 2001 15:38:00 +0100
Subject: Sectioning Ta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to be of some assistance on the subject of a solution
to:-'There is no simple solution to the preparation of very hard and very
soft materials in one specimen.'

As our Bright/Hacker OTF/AS/D/LT cryostat is able to section a hard and soft
specimen incorporated into one specimen. If this is of interest I would be
pleased to receive sample and return our results to those who have a
problem.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com


-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: 07 June 2001 23:26
To: MICROSCOPY BB


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:

====================================================
Tantalum is very hard and tough, so I doubt that it will section with either

diamond or tungsten carbide knives. Preparing the material like a geological

section by grinding is a possibility, but not a good one. Chances are that
the
grinding material would fill the voids and the tissue would be ground away
first (although ProSciTech and others supply diamond grit embedded in
plastic
disks, which are much better in that regard)
Proper vacuum infiltration with a low viscosity resin, cutting with a
diamond
blade, grinding with a diamond disc would be my preparation trial.
After that either stain the surface and try your luck with a high power
reflection (metallurgical) microscope, or more likely digest the plastic and

view the specimen in SEM.
If the tantalum could be fractured open, an ESEM my offer some "insights".
There is no simple solution to the preparation of very hard and very soft
materials in one specimen.
=====================================================
Actually Ta **can** be thin sectioned if it is done by someone with the
proper experience and who is using the right kind of diamond knife. We have
offered our SPI #02888-AB sectioned Ta foil on a grid for AEM studies for
some number of years; see URL
http://www.2spi.com/catalog/standards/aem.html

The Ta system that prompted the original posting was thicker, however, it is
also porous, and if properly infiltrated with resin, and assuming the
porosity is above some point, in principle, at least, there is no reason why
it could not be thin sectioned for TEM. No method is really artifact free,
but some artifacts are more easy to recognize than others. Knife induced
artifacts are anisotripic (e.g. directional) in nature and can be more
easily recognized (as artifacts) than artifacts caused by say, ion milling,
which are isotropic in nature.

When I say "right kind of diamond knife", I am not suggesting that the SPI
diamond knife could do something above and beyond what other diamond knives
could do. What I am saying is that there is a process of selection of the
optimum knife angle, because one might have to be prepared to use a knife
with a fairly low (for materials science work) knife angle, rather than a
more blunt angle, but with the downside is that it will wear out more
quickly. That might make for great business for a diamond knife supplier,
but it does tend to get expensive for the user who is not so experienced
with this kind of sectioning.

Disclaimer: SPI Supplies offers diamond knives, both materials and life
science, and we have done this kind of sectioning, as a service, for
commercial clients for over thirty years.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask that
any reply to this message be by way of the "reply" feature on your software,
so that the entire string of correspondence can come back to us and all be
in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================





From daemon Mon Jun 11 10:56:47 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 11 Jun 2001 10:48:32 -0500
Subject: Re: measurments at LM and TEM levels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
The optics of forming an image is constrained by diffraction
so that when the light comes together in a point it actually has a
finite breadth. This is given by the numerical aperture of your
objective and condenser (and the wavelength of light) and has a
theoretical min of around 250 nm for green light. If you are using a
high NA lens (say 1.3) and an equally good condenser, which must be
oiled to the slide to get its full resolution, then you should be
able to reach this limit. To the extent that your lenses are not that
high NA, the limit will be larger. Vesicles smaller than the limit
may be imaged (indeed, single microtubules can be imaged) but the
diameter will read out on the image as being whatever your
diffraction limit is. You can find a good discussion of this in
Inoué's book on Video Microscopy, to name just one source.

As ever,
Tobias

}
} Hello all,
}
} I have a question about measuring small cellular structures at the light
} microscope level using Argus 10 enhancement and measurement software.
}
} I am measuring vesicle diameters in living cells of the fungus Neurospora.
} They measure about 350-400 nm in diameter using the Argus 10 software.
} With cryofixed TEMs of the same cell type, the only vesicles with the same
} distribution as at the LM level measure 150 nm diameter and there are no
} structures at 350-400 nm.
}
} All my calibrations of both the LM and TEM have been checked and
} double-checked.
}
} It is my understanding that video-enhanced LM can amplify signal (or sizes)
} so that structures below limit of LM resolution can be detected (eg,
} imaging of individual microtubules with DIC/VELM). I am wondering if this
} is the source of this discrepancy that it see and if there are references
} that describe this.
}
} Thanks so much,,,,,,
} Robby Roberson
}
}
} ***************************************
} Robert W. Roberson, PhD
} Department of Plant Biology
} Molecular and Cellular Biology Program
} Arizona State University
} Tempe, AZ 85282
} Office/Lab 480-965-8618

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Mon Jun 11 11:15:16 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 11 Jun 2001 12:09:07 -0400
Subject: RE: Formula for calculating dept of focus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Somebody else already answered the question.

See the fancy URL at "Matter" in the UK.

http://www.matter.org.uk/tem/depth_of_field.htm

Regards, Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Alan J. Kruger
} Sent: Friday, June 8, 2001 1:05 PM
} To: Microscopy
} Subject: LM: Formula for calculating dept of focus
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone lead me to a reference or the formula to calculate dept of
} focus on light microscopes?
}
} Al Kruger
} USDA Meat Animal Research Center
}
}
}


From daemon Mon Jun 11 14:59:33 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 11 Jun 2001 15:55:39 -0400
Subject: Source of used equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A few days ago someone asked about sources for used and renovated
microscopy equipment. Just today I received a leaflet from
Microscopy Laboratories, (P.O. Box 338, Red Bank, NJ 07701,
email:micro-at-mail.superlink.net; Tel: 732-747-6228) that lists a wide
variety of items of reconditioned and guaranteed equipment they are
offering for sale.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Mon Jun 11 18:46:34 2001



From: Richard Easingwood :      richard.easingwood-at-stonebow.otago.ac.nz
Date: Tue, 12 Jun 2001 11:45:05 +1200
Subject: SEM of psoriasis anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
I have been contacted by someone wishing urgently to obtain an image of
skin with psoriasis for a science exhibition aimed at children. They
specified an SEM image but possibly TEM or LM would do. We have nothing
along these lines in our lab - is anyone out there in the wide world of
microscopy able and willing to provide such an image?

Best regards,


Richard

Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: office: 0064 3 479 7301, mobile: 0064 21 222 4759
Facsimile: 0064 3 479 7254
mailto:richard.easingwood-at-stonebow.otago.ac.nz
Web site: http://www.otago.ac.nz/anatomy/emunit/








From daemon Mon Jun 11 21:10:14 2001



From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Tue, 12 Jun 2001 14:03:27 +1300 NZDT
Subject: Re: LM: Formula for calculating dept of focus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On 8 Jun 01, at 12:05, Alan J. Kruger wrote:

} Can anyone lead me to a reference or the formula to calculate dept of
} focus on light microscopes?

Al,

"Photomicrography. A comprehensive treatise" by Roger P. Loveland
John Wiley & Sons, 1970, contains an appendix on calculating
depth of field. For compound microscopes Loveland gives several
formulae, which produce similar but not identical results in his
worked examples.

I can fax it to you if you can't find a copy locally.

Regards

Stephen Edgar

Pathology Department
Faculty of Medicine & Health Sciences
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459


From daemon Tue Jun 12 01:20:59 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 12 Jun 2001 16:15:28 +1000
Subject: RE: Sectioning Ta

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This thread may never finish.
Fred Monson wrote to me making the point that he thought that the original
request (which I have appended last) related to a carbon sponge coated with Ta.
Fred also had and so he appended an article in pdf format, which deals with
such material. There also is what appears to be an SEM image of a thick section
of this very open material. It seems that it had been sectioned, presumably
with a diamond or TC knife. It does however, not show any cell inclusions,
though the article says that purpose of the matrix is the growths of T-cells.
Plastic infiltrated Ta coated C sponge with cell inclusions "may" section.
The original correspondence referred to a Ta sponge coated with carbon with
cell inclusions and that would be a whole lot more difficult.
The publication is:
Contact: Mark J. Pykett, Cytomatrix, (781) 939-0995, mpykett-at-cytomatrix.com
I think that we could have a delightful time with a hundred protagonist writing
to the unsuspecting "Contact"
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, June 12, 2001 12:38 AM, Alan Bright [SMTP:bright-at-dial.pipex.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would like to be of some assistance on the subject of a solution
} to:-'There is no simple solution to the preparation of very hard and very
} soft materials in one specimen.'
}
} As our Bright/Hacker OTF/AS/D/LT cryostat is able to section a hard and soft
} specimen incorporated into one specimen. If this is of interest I would be
} pleased to receive sample and return our results to those who have a
} problem.
}
} Best Regards
}
} Alan Bright
}
} Bright Instrument Co.Ltd.
} St Margaret's Way
} Huntingdon
} Cambridgeshire
} PE29 6EU
} England
}
} Tel No:+44 (0)1480 454528
} Fax No:+44 (0)1480 456031
} Email: AlanBright-at-brightinstruments.com
} Web Site: www.brightinstruments.com
}
}
} -----Original Message-----
} } From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
} Sent: 07 June 2001 23:26
} To: MICROSCOPY BB
} Subject: Sectioning Ta
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
}
} ====================================================
} Tantalum is very hard and tough, so I doubt that it will section with either
}
} diamond or tungsten carbide knives. Preparing the material like a geological
}
} section by grinding is a possibility, but not a good one. Chances are that
} the
} grinding material would fill the voids and the tissue would be ground away
} first (although ProSciTech and others supply diamond grit embedded in
} plastic
} disks, which are much better in that regard)
} Proper vacuum infiltration with a low viscosity resin, cutting with a
} diamond
} blade, grinding with a diamond disc would be my preparation trial.
} After that either stain the surface and try your luck with a high power
} reflection (metallurgical) microscope, or more likely digest the plastic and
}
} view the specimen in SEM.
} If the tantalum could be fractured open, an ESEM my offer some "insights".
} There is no simple solution to the preparation of very hard and very soft
} materials in one specimen.
} =====================================================
} Actually Ta **can** be thin sectioned if it is done by someone with the
} proper experience and who is using the right kind of diamond knife. We have
} offered our SPI #02888-AB sectioned Ta foil on a grid for AEM studies for
} some number of years; see URL
} http://www.2spi.com/catalog/standards/aem.html
}
} The Ta system that prompted the original posting was thicker, however, it is
} also porous, and if properly infiltrated with resin, and assuming the
} porosity is above some point, in principle, at least, there is no reason why
} it could not be thin sectioned for TEM. No method is really artifact free,
} but some artifacts are more easy to recognize than others. Knife induced
} artifacts are anisotripic (e.g. directional) in nature and can be more
} easily recognized (as artifacts) than artifacts caused by say, ion milling,
} which are isotropic in nature.
}
} When I say "right kind of diamond knife", I am not suggesting that the SPI
} diamond knife could do something above and beyond what other diamond knives
} could do. What I am saying is that there is a process of selection of the
} optimum knife angle, because one might have to be prepared to use a knife
} with a fairly low (for materials science work) knife angle, rather than a
} more blunt angle, but with the downside is that it will wear out more
} quickly. That might make for great business for a diamond knife supplier,
} but it does tend to get expensive for the user who is not so experienced
} with this kind of sectioning.
}
} Disclaimer: SPI Supplies offers diamond knives, both materials and life
} science, and we have done this kind of sectioning, as a service, for
} commercial clients for over thirty years.
}
} Chuck
}
original request was posted on 6 June 2001

I am posting this for a college, Lauri Wyner, in the Pathology Core.
I have no idea how to section this stuff...!

Thanks!
Maria Ericsson

My question is:
I have a carbon coated porous tantalum matrix approximately 1 cm in
circumference and 2 mm thick. This matrix has fixed cells adhered to the
surface and throughout. I would like to embed this, make slides and stain
for H&E to confirm the presences of cells as well as immunohistochemistry
to characterize them. I am looking for suggestions on how I can section
this matrix while maintaining its overall structure. Any information would
be greatly appreciated.





From daemon Tue Jun 12 04:33:52 2001



From: chkiang-at-physics.ucla.edu (Ching-Hwa Kiang)
Date: Tue, 12 Jun 2001 02:26:09 -0700 (PDT)
Subject: Postdoc position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL POSITIONS IN NANOTECHNOLOGY AND BIOPHYSICS
AT THE DEPARTMENT OF PHYSICS, UCLA

We are seeking outstanding candidates for postdoctoral
research. Both spectroscopy/microscopy techniques and
a strategic synthetic approach are used for materials
discovery and studying the physics these novel materials.
DNA/gold particles are used to probe the melting and
duplex formation of DNA in confined geometries.
Cryoelectron microscopy is used to study the structure
and function of macromolecular assemblies at the
interface between biological machines and nanotechnology.

Current Research Areas:
Carbon Nanotubes
Molecular biophysics of DNA and proteins
Structure and function of macromolecular assemblies

The compensation package is competitive,
and medical benefits are included.
Interested person should send cv
and three letters of recommendation to:
Professor Ching-Hwa Kiang
Department of Physics & Astronomy
6-130 Knudsen Hall
University of California
Los Angeles, CA 90095-1547
chkiang-at-physics.ucla.edu

-----------------------------------------------------------------
Dr. Ching-Hwa Kiang 6-130 Knudsen
Department of Physics Phone: (310) 206-0563
University of California Fax: (310) 825-5734
Los Angeles, CA 90095-1547 chkiang-at-physics.ucla.edu
http://www.physics.ucla.edu/people/faculty_members/kiang
-----------------------------------------------------------------


From daemon Tue Jun 12 08:49:34 2001



From: Dmrelion-at-aol.com
Date: Tue, 12 Jun 2001 09:39:39 EDT
Subject: test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


test


From daemon Tue Jun 12 09:54:33 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 12 Jun 2001 10:47:12 -0400 (EDT)
Subject: How an FEG manufactured?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello lister,

A fellow here in the department has the interest in knowing by which mean
an FEG W-crystal needle is cut. I only know that in the case of Schottky
emitter, the submicron needle is cut in {100} direction and coated with
ZrO2 to reduce the work function. But I have no knowledge in the
manufacturing process of the tip (FIB?). Anybody who knows please shed
some lights. Thanks in advance.

*********************************
Chaoying Ni
Electron Microscopy Laboratory
Materials Science and Engineering
College of Engineering
University of Delaware
Newark, DE 19716
*********************************



From daemon Tue Jun 12 10:24:13 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 12 Jun 2001 11:18:19 -0400
Subject: Quantitative Metallurgical analysis of Au/Sn in Microcircuit appl

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks;

I have a question as to technique or method regarding the quantification of
Au/Sn in a "solder bump" application for an InP microcircuit. Essentially
what I've been asked to do is provide the weight % of each element after
this "solder" has been melted and re-flowed. The samples are cylindrical in
shape with an x-section of 65 uM and a thickness of 34 uM. The Au/Sn
portion sits atop this structure and is a thickness of ~ 2 uM.

My question is, how can I quantify, in Wt. %, the ratios of the two metals?
I have EDX at my disposal but I am uncertain that I will be able to
accurately quantify this alloy without a standard. One other issue is that
the underlying metallization that primarily constructs this "bump" is also
Au for the next 32 uM.

Any insight into this problem would be greatly appreciated by myself and my
colleagues.

Peter Tomic
Analytical Services Group
Anadigics, Inc.
35 Technology Drive
Warren, New Jersey
U.S.A. 07090



From daemon Tue Jun 12 13:57:07 2001



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Tue, 12 Jun 2001 14:49:11 -0400
Subject: Philips CM-10 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An excellent Philips CM-10 transmission electron microscope is being
offered for sale by Michigan State University. It is 1987 vintage and has
been on service contract since new. We can demo this instrument at any
time. Please contact Dr. Xudong Fan with questions, fanx-at-msu.edu,
517-353-4525.

Stanley L. Flegler, Acting Director
Center for Advanced Microscopy



From daemon Tue Jun 12 15:40:22 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 12 Jun 2001 16:38:50 -0400
Subject: Re: How an FEG manufactured?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Chaoying Ni ,
I don't know about the current crop of FE cathodes, but in the past an
electrolytic cell was set up with the W needle as part of the circuit.
The needle was slowly dipped and removed several times from the NaOH
electrolyte. Essentially, it was the same technique as was (and is)
used to produce micomanipulator needles. I think the set-up was shown
and described well in a Microscopy Today article within the past 12 months.

Ken Converse
owner
Quality Images Delta, PA

Chaoying Ni wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello lister,
}
} A fellow here in the department has the interest in knowing by which mean
} an FEG W-crystal needle is cut. I only know that in the case of Schottky
} emitter, the submicron needle is cut in {100} direction and coated with
} ZrO2 to reduce the work function. But I have no knowledge in the
} manufacturing process of the tip (FIB?). Anybody who knows please shed
} some lights. Thanks in advance.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Laboratory
} Materials Science and Engineering
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************
}
}
}
}



From daemon Tue Jun 12 16:22:37 2001



From: Dale A. Callaham :      dac-at-bio.umass.edu
Date: Tue, 12 Jun 2001 17:18:26 -0400
Subject: JEOL JSM-5200 Wehnelt Assembly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi -

I was cleaning today and I have a complete ready-to-plug wehnelt assembly
for a JEOL JSM-5200 SEM to give away. We inherited this piece and it is
just a bit different from the unit in our 5400, so please only ask if you
really have a 5200 as it probably won't fit other models. Claim goes to the
first school or non-profit that requests it, otherwise others.

Contact me if interested. Be willing to pay postage. ($10???)

Dale Callaham
+++++++++++++++++
Dale A. Callaham
Senior Microscopist, Stuck Jar Lid Opener, Odd Job Bear
Central Microscopy Facility
Morrill 4 North, Room 1
639 North Pleasant Street
The University of Massachusetts
Amherst, MA 01003-9278
-------------------------
Phone 413-545-3751
FAX 413-545-3243
email dac-at-bio.umass.edu
http://www.bio.umass.edu/microscopy


From daemon Tue Jun 12 16:33:56 2001



From: Andrew Kellock :      kellock-at-almaden.ibm.com
Date: Tue, 12 Jun 2001 14:29:20 -0700
Subject: EMP Cameca SX50 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After more than 20 years of faithful service at IBM Research we will be
retiring our Cameca SX50 electron microprobe.
The machine has three wavelength dispersive detectors as well as one energy
dispersive detector. The machine is operational except for the computer
system, which is a PDP-11. The machine has been dormant, but under vacuum
for roughly 3 years.
If anyone is interested in purchasing the machine, please send your offers
to :

Dr Andrew J. Kellock
Ion Beams Lab
Almaden Research Center
(408) 927 2353
kellock-at-almaden.ibm.com



From daemon Tue Jun 12 17:38:37 2001



From: Carl Morten Motzfeldt Laane :      c.m.m.laane-at-bio.uio.no
Date: Tue, 12 Jun 2001 17:35:19 -0500
Subject: Zeiss Photomicroscope II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sirs!

We have an old Zeiss Photomicroscope II at our lab, mainly in wery good
condition, but there are electronic problems with the automatic camera
system. There are some faults in the large electronic box placed in the
microscope table.
We have possibilities to repair the microscope here at the university, but
unfortunately we have only been able to get a copy of the "Gesamtschaltplan
zum Photomicroscope III" (the electronic diagram), but that is entirely
different. In order to repair it we need the original electronic diagram
(or a copy of it) for the Photomicroscope II. The Carl Zeiss Factory could
not help us.
If someone could send us a copy, we would highly appreciate it.

Sincerely:

Morten Motzfeldt Laane
Professor of Biology (electron-microscopy)
Biological Institute
P.O.Box 1066,
0316 Blindern, Oslo, Norway


From daemon Tue Jun 12 17:39:50 2001



From: Dalton.Pierson-at-sealedair.com
Date: Tue, 12 Jun 2001 17:38:01 -0500
Subject: "Hot-stage microscopy"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir or Madame,
I have been asked by my employer to investigate setting my laboratory up to
perform what was called "hot-stage microscopy". I understand this to be the
ability to observe the melting of (in this case) a polymer. I was hoping
that your organization could point me in the right direction. Any
information you could give me would be greatly appreciated.

Thank you,
Dalton Pierson


From daemon Tue Jun 12 17:40:28 2001



From: nancy.meijer-at-uniqema.com
Date: Tue, 12 Jun 2001 17:38:41 -0500
Subject: LM - Need help on staining oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear reader,

I am looking for a colouring agent for oil (emollients) which needs to
applied to human skin.
If we apply the oil as such we do not have enough contrast for
measurements.
This colouring agent needs to be skin friendly, leave no stain on the skin
and it must not
change the viscosity of the oil.

Any information would be very helpful and appreciated.

Thanks,

Nancy Meijer


IMPORTANT NOTICE:
This email may be confidential, may be legally privileged, and is for the
intended recipient only. Access, disclosure, copying, distribution, or
reliance on any of it by anyone else is prohibited and may be a criminal
offence. Please delete if obtained in error and email confirmation to the
sender.


From daemon Tue Jun 12 17:58:24 2001



From: lilaeloise-at-cs.com ()
Date: Tue, 12 Jun 2001 17:55:35 -0500
Subject: Ask-A-Microscopist: laser confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(lilaeloise-at-cs.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
June 12, 2001 at 16:14:22
---------------------------------------------------------------------------

Email: lilaeloise-at-cs.com
Name: Lila Allen

Organization: Sunrise Hospital

Education: Graduate College

Location: Nevada

Question: I've been looking for information regarding the differences
between laser confocal microscope and digital confocal microscopes,
including the advantages/disadvantages and uses of each.
Thank you,
Lila

---------------------------------------------------------------------------


From daemon Wed Jun 13 01:25:38 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 12 Jun 2001 20:15:45 -1000 (HST)
Subject: TEM - effects of osmolarity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied to my request for references to images of
osmolarity problems. The book below is a wonderful resource for images of
all kinds of treatments ranging from the effects of various fixation
protocols to analysis of digital images. I don't know how this book
escaped my notice when it came out, but I'm buying it, and heartily
recommend it!

} Biomedical Electron Microscopy by Maunsbach A B & Afzelius
} B A (1999) has several pages of illustrations of shrinkage
} etc.
}

} } Hi, All-
} }
} } Does anyone have a reference for or some transmission electron micrographs
} } that illustrate the effects of osmolarity of buffers on animal cells? I
} } would like to show students on tour of our facility shrinkage and swelling
} } of cells and organelles. I have other wonderful artifacts to show,
} } including one of my first sections with chatter so bad it looks like
} } mini-blinds...
} }
} } Mahalo!
} } Tina
} }
} } http://www.pbrc.hawaii.edu/bemf/microangela
} }
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Jun 13 01:48:46 2001



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Wed, 13 Jun 2001 06:45:08 GMT
Subject: Unwanted Newton rings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
} From time to time I need to scan some negatives with flat bed scanner
(Umax PowerLook II). Sometimes there is a problem with Newton rings in
the scanned image. Does anybody know some tricks how to solve this
problem?
Best regards Oldrich
+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of Electron Microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm




From daemon Wed Jun 13 06:29:49 2001



From: Tom Isabell :      isabell-at-emispec.com
Date: Wed, 13 Jun 2001 06:25:02 -0500
Subject: Open Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Technical Sales Engineer

Emispec Systems, Inc., a rapidly growing company that creates
software for electron microscopy, is looking for a domestic technical
sales engineer. We are looking for someone with strong
interpersonal skills and experience with customer interactions.

Some of the job requirements will include:

-Strong technical knowledge of products.
-Ability to give product demonstrations at tradeshows and at customer sites.
-Providing product information and quotations.
-Defining potential customers.
-Qualifying leads and determining customer needs.
-Adding/updating contact information in the sales database.
-Strong customer support throughout the purchasing cycle and after.
-Handling initial customer service requests, and coordinating with
the service department to see that they are handled appropriately.
-Extensive travel.

The ideal candidate should have a college degree. Sales experience
and/or experience in electron microscopy is a plus.
Salary is commensurate with experience.

Emispec Systems, Inc. is an equal opportunity employer.

Resumes and salary requirements should be sent to:
Human Resources
Emispec Systems, Inc.
2050 S. Cottonwood Drive
Tempe, AZ 85282
Attn: Technical Sales

Resumes will also be accepted via email to:
hr-at-emispec.com.


From daemon Wed Jun 13 06:33:27 2001



From: ggauss-at-dynacocorp.com ()
Date: Wed, 13 Jun 2001 06:31:34 -0500
Subject: Ask-A-Microscopist:looking for a manual JENA Jenavert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(ggauss-at-dynacocorp.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
June 12, 2001 at 18:12:49
---------------------------------------------------------------------------

Email: ggauss-at-dynacocorp.com
Name: Gordon Gauss

Organization: Dynaco Corporation

Education: Undergraduate College

Location: Tempe, Arizona

Question: We recently received a microscope with no manual. It is an
aus JENA Jenavert. It is in excellent condition. We believe it was
East German made. Is anyone familiar with this microscope and how can
we obtain a manual in either german or english. Thank you...

---------------------------------------------------------------------------


From daemon Wed Jun 13 06:42:01 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 13 Jun 2001 06:28:05 -0500
Subject: RE: Unwanted Newton rings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If Newton rings are the same as Moire patterns (constructive/destructive
interference lines)....

Many scanner drivers have a "de-screen" option or the like. My Microtek
system seems to do a decent job of removing the lines. Also, you might try
scanning at a much higher dpi (dots per inch ...or cm), then lightly average
pixels and reduce in array size to meet needs.

Woody White
McDermott Technology, Inc

} --------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hello,
} } From time to time I need to scan some negatives with flat
} bed scanner
} (Umax PowerLook II). Sometimes there is a problem with Newton
} rings in
} the scanned image. Does anybody know some tricks how to solve this
} problem?
} Best regards Oldrich
} +-----------------------------------+
} Oldrich Benada
} Acad. Sci. CR
} Institute of Microbiology
} Laboratory of Electron Microscopy
} Videnska 1083
} CZ - 142 20 Prague 4 - Krc
} Czech Republic
} +------------------------------------+
} Phone: +420-2-4752399
} Fax: +420-2-4715743
} WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm
}
}
}


From daemon Wed Jun 13 08:10:52 2001



From: Kathy Wolken :      wolken.1-at-osu.edu
Date: Wed, 13 Jun 2001 09:15:22 -0400
Subject: University service lab charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I run a research based service lab at Ohio State University and I've been
asked to find out what other universities charge their internal customers
for the use of TEM, SEM, confocal microscopy and technical assistance. I'm
particularly interested in biological labs at large state universities, big
10 universities, and Ohio universities. Please respond directly to
me. Thanks.
Kathleen S. Wolken
Senior Electron Microscopist
Campus Microscopy and Imaging Facility (CMIF)
4029 Graves Hall
333 West 10th Avenue
Columbus, OH 43210-1239

Phone 614-292-9786
FAX 614-688-8742
WEB http://www.med.ohio-state.edu/cmif




From daemon Wed Jun 13 08:29:12 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 13 Jun 2001 09:23:11 -0400
Subject: Unwanted Newton rings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Newton rings are interference fringes due to the small air gap when the film not quite touches the glass. The way to get around the problem is to raise the film off of the glass just slightly. Most of the better quality scanners provide holders, although not TEM sized one. Make your own from two pieces of a file folder cut to the appropriate size and held together at one edge with tape. There is no problem with a defocus of the image.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Oldrich Benada [mailto:benada-at-biomed.cas.cz]
Sent: Wednesday, June 13, 2001 2:45 AM
To: microscopy-at-sparc5.microscopy.com


Hello,
} From time to time I need to scan some negatives with flat bed scanner
(Umax PowerLook II). Sometimes there is a problem with Newton rings in
the scanned image. Does anybody know some tricks how to solve this
problem?
Best regards Oldrich
+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of Electron Microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm




From daemon Wed Jun 13 08:30:47 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 13 Jun 2001 09:27:19 -0400
Subject: Re: How an FEG manufactured?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try speaking to Hitachi in Gaithersburg, MD. Their Applications Lab. should
have that info. at their fingertips.

Peter Tomic
Anadigics

-----Original Message-----
} From: Ken Converse [mailto:qualityimages-at-netrax.net]
Sent: Tuesday, June 12, 2001 4:39 PM
To: Chaoying Ni; MSA, listserver



Chaoying Ni ,
I don't know about the current crop of FE cathodes, but in the past an
electrolytic cell was set up with the W needle as part of the circuit.
The needle was slowly dipped and removed several times from the NaOH
electrolyte. Essentially, it was the same technique as was (and is)
used to produce micomanipulator needles. I think the set-up was shown
and described well in a Microscopy Today article within the past 12 months.

Ken Converse
owner
Quality Images Delta, PA

Chaoying Ni wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello lister,
}
} A fellow here in the department has the interest in knowing by which mean
} an FEG W-crystal needle is cut. I only know that in the case of Schottky
} emitter, the submicron needle is cut in {100} direction and coated with
} ZrO2 to reduce the work function. But I have no knowledge in the
} manufacturing process of the tip (FIB?). Anybody who knows please shed
} some lights. Thanks in advance.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Laboratory
} Materials Science and Engineering
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************
}
}
}
}



From daemon Wed Jun 13 10:01:43 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 13 Jun 2001 10:55:00 -0400
Subject: Reichert & LKB Ultramicrotome trade

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I am looking for a LKB Nova ultramicrotome. I presently have a Reichert
Ultracut E which works absolutely fine, its just that I'm really a fan of
LKB ultramicrotomes (the last one was a LKB III which is not usable
anymore). I have used LKB's for over 20 yrs and I would prefer to go back
to that brand. Does anyone out there want to swap or sell one?

Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642
716-275-1954


From daemon Wed Jun 13 10:01:43 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 13 Jun 2001 15:54:52 +0100 ()
Subject: Re: "Hot-stage microscopy"

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Dalton,

We have been looking at melting and crystallization of polymers under
hot stages for twenty years. We have always found Mettler-Toledo Hot
Stages very good, and have bought three of them.

For information, go to:

http://www.mt.com

then click on "Products" in the bar at top;

in the window 2.Product Search type "stage";

in the next window, under the text "Thermal Data - FP82HT/FP84HT"

click on the little button "English"

which will take you to a screen where you can request more information;

or else go directly there with

http://www.mt.com/home/products/glo/scripts/view/viewproduct.asp?LanguageCode=EN&RecNo=1000000734

(that's all one line, in case the e-mail server has chopped it in two.)

** Disclaimer ** we have no commercial interest in M-T, simply we've found
them very good and helpful.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

On Tue, 12 Jun 2001 Dalton.Pierson-at-sealedair.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear Sir or Madame,
} I have been asked by my employer to investigate setting my laboratory up to
} perform what was called "hot-stage microscopy". I understand this to be the
} ability to observe the melting of (in this case) a polymer. I was hoping
} that your organization could point me in the right direction. Any
} information you could give me would be greatly appreciated.
}
} Thank you,
} Dalton Pierson
}
}



From daemon Wed Jun 13 10:50:17 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 13 Jun 2001 11:46:45 -0400
Subject: Re: Quantitative Metallurgical analysis of Au/Sn in Microcircuit appl

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Dear Peter,

You do not indicate whether the objective is to measure the total amount
of the two metals in the assembly as a whole, or whether you want to
measure their proportions in each of the different areas: the tin
solder, the intermetallic layers and so on. You also don't indicate the
purpose to which the results are to be put. To some extent the answer
depends on knowing this, as a biased but highly repeatable measure of
something is often useful for internal purposes.

Someone with specific experience in this industry might have a more
specific reply but in general, the problem is that the same elements are
shared by very different phases in close proximity to one another. For
the Au/Sn intermetallic compounds which have quite specific
compositions, the absorption /enhancement effects will be different than
for a low-concentration solid solution of either of the metals in the
other. Without a standard comprised of a known intermetallic phase, the
results could be consistent (that is, you might be able to use the
measure as a means of process control) but not very accurate (as
compared with the absolute amounts present). Another frequently
overlooked problem with quantifying the results of e-probe analysis of a
multiphase alloy is that the size distribution of imiscible but
intimately mixed phases may change due to process variables (e.g.
thermal history) even though the total proportion of the metals remains
the same. The micro-environment (matrix effect) experienced by an
emitted x-ray will depend on its probability of passing through the same
material or a different phase on its way to reaching the detector.
Obviously this can be affected by the grainsize of a eutectic mixture.

You haven't indicated whether the sample is to be sectioned into a
planar surface for analysis or examined as is. Another frequently
overlooked problem is that of x-rays emitted through secondary
interactions with structures that are not illuminated by the beam but
which are "visible" within the detector collimator's view. A planar
cross section presents the least chance for interference of this kind.
On the other hand, a gold-metallized surface projection that resides
next to your solder structure could be a source of enhanced Au-m x-rays
produced through secondary fluorescence by the Sn-l x-rays under the
beam. (even though secondary emmission is not a very efficient process,
the number of Sn x-rays fluorescing the adjacent gold is much higher
than the number that happen to be emitted in the direction of the X-ray
detector. Meanwhile the X-ray detector is looking at the whole scene
indiscriminately.

John Twilley

Peter Tomic wrote:

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} -----------------------------------------------------------------------.
}
}
} Folks;
}
} I have a question as to technique or method regarding the quantification of
} Au/Sn in a "solder bump" application for an InP microcircuit. Essentially
} what I've been asked to do is provide the weight % of each element after
} this "solder" has been melted and re-flowed. The samples are cylindrical in
} shape with an x-section of 65 uM and a thickness of 34 uM. The Au/Sn
} portion sits atop this structure and is a thickness of ~ 2 uM.
}
} My question is, how can I quantify, in Wt. %, the ratios of the two metals?
} I have EDX at my disposal but I am uncertain that I will be able to
} accurately quantify this alloy without a standard. One other issue is that
} the underlying metallization that primarily constructs this "bump" is also
} Au for the next 32 uM.
}
} Any insight into this problem would be greatly appreciated by myself and my
} colleagues.
}
} Peter Tomic
} Analytical Services Group
} Anadigics, Inc.
} 35 Technology Drive
} Warren, New Jersey
} U.S.A. 07090
}
}
}
}



From daemon Wed Jun 13 11:06:36 2001



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Wed, 13 Jun 2001 10:00:50 -0600
Subject: RE: Unwanted Newton rings

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Hi,
I've found two things that helped eliminate this problem. If you place the
negative directly onto the scanner bed, use an anti-static brush to wipe
both the bed and the negative first. The second solution was to modify a
negative holder. I work mostly with TEM sized negatives and we just cut out
the back (solid) part of a TEM negative holder, then we place the negative
into the holder which keeps it from contacting the scanner bed directly. If
your working with a different size negative you could fashion a holder from
anything, even a piece of poster board should work. I think the fringes
are caused by a build up of static between the scanner bed and the negative.
I have also found that if you go to adjust contrast in print shop and adjust
the contrast through the entire range from too light to too dark you can
pick up a fringe before you take the negative off the scanner. That
eliminates having to go back and re-do a picture because the fringe wasn't
real apparent when you scanned it. Hope this works for you...it has made my
life a lot easier.
Dorrance

} ----------
} From: Oldrich Benada
} Sent: Tuesday, June 12, 2001 11:45 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Unwanted Newton rings
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hello,
} } From time to time I need to scan some negatives with flat bed scanner
} (Umax PowerLook II). Sometimes there is a problem with Newton rings in
} the scanned image. Does anybody know some tricks how to solve this
} problem?
} Best regards Oldrich
} +-----------------------------------+
} Oldrich Benada
} Acad. Sci. CR
} Institute of Microbiology
} Laboratory of Electron Microscopy
} Videnska 1083
} CZ - 142 20 Prague 4 - Krc
} Czech Republic
} +------------------------------------+
} Phone: +420-2-4752399
} Fax: +420-2-4715743
} WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm
}
}
}
}
}



From daemon Wed Jun 13 11:32:58 2001



From: mario:      jane0l2l-at-excite.com
Date: Wed, 13 Jun 2001 11:37:25
Subject: Manuf. Production/Contrl Software For $1,495.00.

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Job Master, a complete, user friendly Windows based software package, can manage and control your operation from sales quote to shipment.

For one week only, Job Master, normally $2,495.00, is on sale for a total price of $1,495.00. In order for you to receive this $1,000.00 savings we must have your order by June 22th.

Job Master is designed specifically for small to medium sized manufacturers, and costs many thousands of dollars less than any other even remotely comparable software package.

Following is a list of features. If you have any questions, would like to discuss the package further, or if you would like to obtain our Web site address for a total walk through of the program, please call me directly at (661)254-9926(please do not E Mail me back. I may not get your message if you simply hit "Reply" and respond to this message via return E Mail).

By way of background, we are a software company, which for some years has specialized in the development of custom software, primarily for small to medium sized manufacturers. Job Master is a distillation of over a million and a half dollars of software we have developed to control and manage the production of our manufacturing clients.

Job Master contains the following features:

1. QUOTATION MODULE. In this module, quotes are developed, modified, and produced for sending to your client. A history is kept of all quotes for future reference, or modification for other clients. All quotations and revisions are "auto numbered," including versions. The quotes section allows for the entry of parts/processes, and costing of each, including materials, labor, markup, and taxes. Inventory status can be accessed from this section for reference.

2. SALES ORDER. Once a quotation is accepted, the final quotation information can be transformed into a Sales Order for your client's signature on a "point and click" basis. The Sales Order can be modified and re issued if necessary. A history if kept of all Sales Orders for future reference, or modification for other clients. All sales orders and revisions are "auto numbered," including versions. Inventory status can be accessed from this section for reference.

3. CUSTOMER LETTERS can be created from the Quotation and Sales Order sections.

4. SHOP TRAVELER/WORK ORDER. Once a Sales Order is accepted, the sales order information can be transformed into a shop traveler/work order on a "point and click" basis. Each item on the Sales Order becomes a shop traveler/work order, with each step of production of the item then listed on the traveler/work order. Each such traveler/work order is tied back into the Sales Order. The shop traveler/work order allows for the entry of line items, and notes on each line item. The shop traveler/work order contains a "notes" section. The Shop traveler/work order allows for the storing or attachment of drawings to the traveler/work order. The shop traveler/work order also contains a "drop down," from which standard processes can be selected for inclusion on the shop traveler/work order. The shop traveler/work order numbers progress in order of production sequence, and re numbers them if new steps are added. The shop traveler/work order allows for change orders or revisions, and numbers changes in sequence of
he original shop traveler/work order number; i.e., 100, 100-1, 100-2, etc. All shop traveler/work orders and related revisions are retained in memory for future reference. The shop traveler/work order is bar coded for tracking of production step by step, and production of ongoing client status reports. Bar coding includes the ability for an employee to "swipe" their own ID bar code for recording in the system as to who upgraded what step. The shop traveler/work order function also allows for manual update of production status. The shop traveler/work order allows for quality control sign off, and the final production of certifications, either from a "canned" list, or hand typed in on a case by case basis.

5. INVENTORY. The application includes an inventory section, which allows operations to check materials inventory in and out. The inventory section allows for the comparison of inventory received against a P.O., and produces an "overage/underage" report of inventory received as compared against the P.O. The inventory section allows for the setting of minimum (re-order now!) and maximum inventory amounts, and produces reports showing what inventory needs to be ordered, as well as inventory that is at or above the maximum set to have in house. The inventory section also tracks "partially shipped" orders, which are tied in to the shipping function. This section shows how much completed product under a particular order has been actually shipped to a client, and how much remains to be shipped. The balance is adjusted as shipments are made.

6. REQUEST FOR PURCHASE. The application allows operators to produce a Request For Purchase for accounting for any inventory items, which need to be ordered. Inventory items have a drop down of approved vendors for each item.

7. REQUEST FOR BID. The application allows operators to produce a Request For Bid for accounting to send to Vendors for any inventory items, which need to be ordered. Inventory items have a drop down of approved vendors for each item to which Requests For Bid can be sent.

8. INVOICE. The application produces an invoice/invoice detail for all completed items ready to be billed/shipped to clients.

9. PRODUCTION OUTPUT STATUS. The application produces a date range selectable report on how much product, and the value of the product, which was completed during a selected date range. The application also produces a report on how many orders, and the value of those orders, which remain to be completed during a selected date range.

10. The application produces SHIPPING DOCUMENTS as per selected shippers, and produces a PACKING SLIP.

11. The application has a "FIND" FUNCTION in selected sections, allowing for searches by customer name, work order number, etc.

12. The application has "AUTO FILL;" i.e., when an operator starts to type in a name, number, etc. all related information auto fills after the first few letters or numbers are typed in.

Job Master is currently being sold in the marketplace for $2,495.00 per package. However, if we receive your order by June 22th, your total price will be $1,495.00.

Again, if you have any questions at all, or would like to place your order, please call me on my direct line, (661) 254-9926. Thank you!


Mario Chavez
Application Sales, Inc.

----------------------------------------------------------------------

You have received this newsletter because you signed up for updates on our tracking software. If you want to unsubscribe from this newsletter, please send a reply email with "REMOVE" in the subject line.


From daemon Wed Jun 13 15:21:11 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 13 Jun 2001 16:15:09 -0400
Subject: RE: Used Instruments

Contents Retrieved from Microscopy Listserver Archives
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Listers: Here's info about another source of used instruments.

- - - - - - - - - - - -


} From: "A. Greene" {ablue-at-io.com}
} To: {bigelow-at-umich.edu}
} Subject: Source
} Date: Mon, 11 Jun 2001 20:05:13 -0500
} X-Priority: 3
} Status:
}
} Hello Professor Bigelow,
}
} Here is another source, as well. I have been in the instrument
} business for over 30 years, used to be with Philips and then at the
} University of Illinois for 10 years. Now, for the past eight years
} have had my own little business in Texas. I probably have more
} parts for older Philips microscopes than Philips. Also, I sell
} reconditioned, used electron microscopes of most brands.
}
} Regards,
}
} Alex Greene
} SCIENTIFIC INSTRUMENTATION SERVICES, INC.
} PMB-499, 1807 West Slaughter Lane, Number 200
} Austin, Texas 78748-6200
} Phone 512/282-5507 FAX 512/280-0702
}
} Sustaining Member - MICROSCOPY SOCIETY OF AMERICA

--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Jun 13 15:50:10 2001



From: Sandra Hancock :      skperkin-at-vt.edu
Date: Wed, 13 Jun 2001 16:48:10 -0400
Subject: Tissue culture/fluorescent antibody staining

Contents Retrieved from Microscopy Listserver Archives
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Hello-

We have a graduate student who is using a fluorescent antibody labelling
technique on a neuronal cell culture plate. Briefly, she fixes the cells
in 4% paraformaldehyde, permeabilizes them with Triton-X and continues with
the immunostaining. The cells stay on the plate (they can only grow on
plates, not slides or coverslips). The question we have is how to
coverslip them and then observe them with a fluorescent microscope. Will
glycerol work? Can we simply put a drop of glycerol on the plate and add a
coverslip or do we need a fluorescent mounting medium? I have limited
experience with fluorescent microscopy, so I would appreciate any help that
you can provide.

Thank you very much,
Sandy Hancock

Laboratory for Neurotoxicity Studies
VMRCVM
VA Tech




From daemon Wed Jun 13 16:05:24 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 13 Jun 2001 14:00:08 -0700
Subject: RE: Unwanted Newton rings

Contents Retrieved from Microscopy Listserver Archives
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I am using transparency for Xerox machine with "windows" cut in it in such
manner, that it's slightly smaller than actual negative's size. In my
particular case I find that rings happens between negative and scanners
bed. So, I put transparency on the bed and than negatives (4 per
transparency sheet) in the way, when negatives situated across the
"windows". In my particular case, transparency presence does not affect
scanned image quality. You may put second sheet over the negatives if
necessary.

Good luck!

Sergey.


At 10:00 AM 6/13/01 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Jun 13 16:22:56 2001



From: tbargar-at-unmc.edu
Date: Wed, 13 Jun 2001 15:09:44 -0500
Subject: Destaining a thin section

Contents Retrieved from Microscopy Listserver Archives
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Hello;

Is it possible to destain a thin section? After having stained with uranyl
acetate and lead citrate. All help appreciated, thanks.

Tom Bargar
EM Lab, UNMC
402-559-7347



From daemon Thu Jun 14 02:07:55 2001



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Thu, 14 Jun 2001 06:58:57 GMT
Subject: Unwanted Newton rings - thanks

Contents Retrieved from Microscopy Listserver Archives
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Many thanks for all replies.
Oldrich Benada


From daemon Thu Jun 14 04:40:23 2001



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Thu, 14 Jun 2001 11:36:36 +0200
Subject: SEM osmolarity

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Hello everybody,

Recently, there was a question about TEM and osmolarity. I have now a
question about SEM and osmolarity. Has anyone have some references about
this topic ? We are studying the canine uterus. We have some problems
concerning (we think !) the osmolarity & the epithelial cells of the
uterus.
Any comment would be appreciated !


Bart De Pauw
Morphology
Belgium



From daemon Thu Jun 14 07:45:06 2001



From: Karl Garsha :      mpalmer1-at-wi.rr.com
Date: Thu, 14 Jun 2001 07:44:26 -0500
Subject: Tissue culture/fluorescent ab staining

Contents Retrieved from Microscopy Listserver Archives
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Greetings Sandy,

Photobleaching, or fading, causes loss of fluorescent signal in part due to
photodynamic events which involve the interaction of the fluorophore with
light energy and oxygen.
Fluorescent labels have a characteristic life span, for instance, an average
fluorescein molecule will emit 30k to 40k photons during its photo-chemical
life span. Higher intensity light excites the fluorophor to emit a greater
yield of photons and the service life of the fluorescent label is
proportionally reduced.
For this reason, it is generally a good idea to add some form of
bleaching protection (anti-fading reagent) to a glycerol-based mounting
media for fluorescent microscopy. P-phenylenediamine (Sigma Chemical Co.)
used in a concentration of 0.1% in [10% phosphate buffered saline/90%
glycerol] is good. Diazabicyclo-octane or n-propyl-gallate (~0.1-0.2% in
10% PBS/Glycerol are decent alternatives. The disadvantage to this approach
is that the coverslip must be sealed (nail varnish) in order to fix the
coverslip permanently.
Vectrashield (Vector Co.) is a propriety product for fluorescent
microscopy which seems to work quite well with the added advantages of
convenience and greater permanence.
One other consideration is the degree to which your fixation protocol
affects the antigenicity of your target-a short (10 min/10% buffered
formalin) is a good place to start-some antigens seem to be very sensitive
to the cross-linking effects of aldehyde fixation, acetone or
ethanol/methanol fixation may prove helpful if this is the case. If the
target is a membrane protein one wants to be cautious with the Triton-X.
Residual Triton-X could also interfere with the antigen-antibody interaction
in a sensitive system. Acetone also permeablizes cell membranes, and if I
recall correctly is a favorable fixative for immunofluoresent studies of the
cytoskeleton.
Culturing cells on slides or coverslips may not be an issue as long as
you can get the culure plates into the scope. However, I believe theromonox
(sp?) coverslips are designed to allow cell adhesion. Some workers coat
slides and/or cover slips with poly-l-lysine for cell adhesion.
Coverslipping is important to allow the optical system of the microscope to
function at full potential. This is less of an issue with inverted
microscopes designed to accomodate tissue culture vessels.
Regards,
Karl Garsha

keg-at-uwm.edu
www.uwm.edu/~keg












From daemon Thu Jun 14 07:46:40 2001



From: Richard Cole :      rcole-at-wadsworth.org
Date: Thu, 14 Jun 2001 08:42:03 -0400
Subject: Destaining a thin section

Contents Retrieved from Microscopy Listserver Archives
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You can de-stain both thick and thin section w/ 0.2 M EDTA. Only the time
differs. One caveat, if the section has been irradiated, i.e. in the TEM
then this will much less effective.

Good luck

Richard Cole
Research Scientist III
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-486-4901 Fax


-----Original Message-----
} From: "tbargar-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"tbargar-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Wednesday, June 13, 2001 4:10 PM
To: Microscopy-at-sparc5.microscopy.com


Hello;

Is it possible to destain a thin section? After having stained with uranyl
acetate and lead citrate. All help appreciated, thanks.

Tom Bargar
EM Lab, UNMC
402-559-7347




From daemon Thu Jun 14 09:31:00 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Thu, 14 Jun 2001 10:23:27 -0400
Subject: University service lab charges

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Dear Kathy:

At the University of Rochester Electron Microscope Research Core, we charge
$75.00/hour with an Electron Microscopist running & photographing using our
Hitachi 7100 TEM, without the EM person, a trained researcher or grad
student must be trained on its use and the rate becomes $50.00/hour.

Our processing and preparation of tissue is at $75.00/hour and that only
counts the hands-on times. For instance, usually it could take 30 minutes
to do 1 micron sections and thin section the same block. We bill for
.5hr=$37.50 to cut one block, .25 hr=$18.75 if no screening 1 micron
sections are needed.

Most investigators have us embedd 2-6 blocks of tissue, etc. We cut a
representative block, review the 1 micron with them if necessary and then
proceed with the thin-sectioning. Staining is usually .25hr...etc, If the
investigator wanted more than one block cut and thin sectioned(3-5 grids),
they would all be stained together using a Hiroka staining kit(holds up to
40 grids) and therefore would only be charged the .25hr rate. However, the
methods and materials you use and speed in which they are performed can vary
from one lab to another.

We only billed $35,000 last year and that doesn't begin to cover salaries.
So the University of Rochester has to subsidize the EM Core. However, the
Core(s) is a big marketing tool for those researchers which are actively
being recruited. Many Molecular Science researchers utilize the EM Core for
ImmunoEM procedures. In fact, the majority of my work is performing pre-
and postembedding immunolabeling for EM. The average bill for this varies
from $00-1,000.

Hope this helps you.

Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642

-----Original Message-----
} From: Kathy Wolken [mailto:wolken.1-at-osu.edu]
Sent: Wednesday, June 13, 2001 9:15 AM
To: microscopy-at-sparc5.microscopy.com


I run a research based service lab at Ohio State University and I've been
asked to find out what other universities charge their internal customers
for the use of TEM, SEM, confocal microscopy and technical assistance. I'm
particularly interested in biological labs at large state universities, big
10 universities, and Ohio universities. Please respond directly to
me. Thanks.
Kathleen S. Wolken
Senior Electron Microscopist
Campus Microscopy and Imaging Facility (CMIF)
4029 Graves Hall
333 West 10th Avenue
Columbus, OH 43210-1239

Phone 614-292-9786
FAX 614-688-8742
WEB http://www.med.ohio-state.edu/cmif




From daemon Thu Jun 14 10:35:09 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 14 Jun 2001 10:24:42 -0500
Subject: Re: Tissue culture/fluorescent ab staining

Contents Retrieved from Microscopy Listserver Archives
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As a general rule, I am less than thrilled about using anti-fade
agents for fixed cells unless absolutely necessary for the reasons
explained below. Live cell work may require anti-oxidants but I am
less familiar with this application. I think it is better to use more
stable fluorochromes (e.g., Alexa 488 instead of FITC)

PPD is a skin sensitizer, photosensitive, and Giloh & Sedat (1982)
Science 217:1252 says it has no effect on rhodamine fading. I
believe they actually showed storage of rhodamine specimens in 5%
n-propyl gallate (NPG) in glycerol for 2-3 days decreased
fluorescence (suggest rinsing slides for storage (what a pain!).
Krienk et al., 1989 J. Immuno. Methods 117:91 compared DABCO, PPD and
NPG and concluded DABCO the least effect. Valnes & Brandtzaeg (1985)
J Histochem Cytochem 33:755 also said DABCO not as effective as PPD
or NPG but also say NPG and PPD only good if slides made recently and
examined promptly - recommended re-mounting in PVA without quencher
if you were going to store. I have tried Vectashield and other
commercial anti-fade compounds with ok results but it is hard to say
exactly how much they helped. I don't think there are any systematic
published studies examining if they work equally well with all
fluorochromes or if they hurt the sample with storage.

Be cautious about using FITC in any PBS buffered mounting medium
since it is much more fluorescent at alkaline (pH 8.6) than neutral
(pH 7.0) pH's.

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Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
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3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Jun 14 10:36:01 2001



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Thu, 14 Jun 2001 10:31:42 -0500
Subject: DAB for Epon-Araldite

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I'm trying to embed and section mouse cochleas that have been immunoreacted
en bloc for BrdU with DAB as the chromagen. The soft tissues of the cochlea
were separated from the bony structures, leaving a soft tissue spiral
containing the organ of Corti.

During processing for Epon-Araldite for 5 micron sections for light
microscopy (without osmium and using increasing concentrations of aqueous
acetone as the dehydrant), it appears as if the chromagen was lost (no
labeled cells were seen) and a purple/grey background was left behind.
Whole mounts immunoreacted at the same time retained an amber coloring with
dark brown-labeled nuclei.

Does anyone have any hints as to what happened here or what we can do in the
future to retain the labeling throughout plastic processing. I've done this
embedding in the past with the same type of tissue (but still in the bony
labyrinth) and had no problems.

Thank you,

Jaclynn M. Lett, Research Assistant
jlett-at-cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314.977.0257 fax: 314.977.0030



From daemon Thu Jun 14 11:27:17 2001



From: L. Muffley :      muffley-at-u.washington.edu
Date: Thu, 14 Jun 2001 09:22:43 -0700 (PDT)
Subject: Re: Tissue culture/fluorescent ab staining

Contents Retrieved from Microscopy Listserver Archives
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Just an FYI-
we have found thermanox to be autoflourescent.

Lara Muffley
Dermatology Dept
University of Washington
Seattle, WA
muffley-at-u.washington.edu

On Thu, 14 Jun 2001, Karl Garsha wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings Sandy,
}
} Photobleaching, or fading, causes loss of fluorescent signal in part due to
} photodynamic events which involve the interaction of the fluorophore with
} light energy and oxygen.
} Fluorescent labels have a characteristic life span, for instance, an average
} fluorescein molecule will emit 30k to 40k photons during its photo-chemical
} life span. Higher intensity light excites the fluorophor to emit a greater
} yield of photons and the service life of the fluorescent label is
} proportionally reduced.
} For this reason, it is generally a good idea to add some form of
} bleaching protection (anti-fading reagent) to a glycerol-based mounting
} media for fluorescent microscopy. P-phenylenediamine (Sigma Chemical Co.)
} used in a concentration of 0.1% in [10% phosphate buffered saline/90%
} glycerol] is good. Diazabicyclo-octane or n-propyl-gallate (~0.1-0.2% in
} 10% PBS/Glycerol are decent alternatives. The disadvantage to this approach
} is that the coverslip must be sealed (nail varnish) in order to fix the
} coverslip permanently.
} Vectrashield (Vector Co.) is a propriety product for fluorescent
} microscopy which seems to work quite well with the added advantages of
} convenience and greater permanence.
} One other consideration is the degree to which your fixation protocol
} affects the antigenicity of your target-a short (10 min/10% buffered
} formalin) is a good place to start-some antigens seem to be very sensitive
} to the cross-linking effects of aldehyde fixation, acetone or
} ethanol/methanol fixation may prove helpful if this is the case. If the
} target is a membrane protein one wants to be cautious with the Triton-X.
} Residual Triton-X could also interfere with the antigen-antibody interaction
} in a sensitive system. Acetone also permeablizes cell membranes, and if I
} recall correctly is a favorable fixative for immunofluoresent studies of the
} cytoskeleton.
} Culturing cells on slides or coverslips may not be an issue as long as
} you can get the culure plates into the scope. However, I believe theromonox
} (sp?) coverslips are designed to allow cell adhesion. Some workers coat
} slides and/or cover slips with poly-l-lysine for cell adhesion.
} Coverslipping is important to allow the optical system of the microscope to
} function at full potential. This is less of an issue with inverted
} microscopes designed to accomodate tissue culture vessels.
} Regards,
} Karl Garsha
}
} keg-at-uwm.edu
} www.uwm.edu/~keg
}
}
}
}
}
}
}
}
}
}
}
}



From daemon Thu Jun 14 12:28:49 2001



From: Pankaj Kumar Patro :      pankaj-at-met.iitb.ac.in
Date: Thu, 14 Jun 2001 22:50:21 -0500 (GMT)
Subject: TEM, etchant for ceramics

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Dear Friends

I am looking for an etching agent for a ceramic
material so that the TEM investigation can be done.

The ceramic material is STRONTIUM BARIUM NIOBATE.

i tried to do it by using HF,but hardly got any
success.

If any one among you has already used some etchant for
this material or related materials are requested to
give some suggestions.

Suggestions for any books or websites to be refeerred
for this purpose are also welcome.




Thanking You

With Regards

Pankaj Kumar Patro

Dept of Met.Engg. and Materials Sc
IIT- Bombay
India- 400076

e-mail-pankaj-at-met.iitb.ac.in




From daemon Thu Jun 14 15:25:32 2001



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Thu, 14 Jun 2001 15:18:30 -0500
Subject: ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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I am in the market to purchase a new ultramicrotome. I am aware of the Leica
and RMC ultramicrotomes. Are there other companies manufacturing
ultramicrotomes today?



John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Thu Jun 14 16:28:23 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Thu, 14 Jun 2001 17:22:35 -0400
Subject: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
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Hi lister,

I have some problems with our EM chillers. The water in EM cooling
circulation is bad contaminated by algae, bacteria, maybe more
microorganisms. Does somebody here know some water cleaner for chillers of
EM? Appreciate for the help.

Haixin Xu

Biological Sciences
University of Maryland, Baltimore County
Baltimore, Maryland



From daemon Thu Jun 14 19:26:22 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Thu, 14 Jun 2001 18:20:07 -0600 (MDT)
Subject: Re: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
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Do you have filters somewhere in the recirculation lines? A lot of places
have 2 barrel-type filters (I don't know the correct term - they
look like tubing, not flat membranes) in-line to clean up anything that
might grow. Change them every 6 months or when they start to look like
they might become sentient.

I've been told that you should not put algicides in the water; the filters
are probably the best way to go.

Tamara

On Thu, 14 Jun 2001, Haixin Xu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi lister,
}
} I have some problems with our EM chillers. The water in EM cooling
} circulation is bad contaminated by algae, bacteria, maybe more
} microorganisms. Does somebody here know some water cleaner for chillers of
} EM? Appreciate for the help.
}
} Haixin Xu
}
} Biological Sciences
} University of Maryland, Baltimore County
} Baltimore, Maryland
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Thu Jun 14 22:33:20 2001



From: Meg Mitchell :      MMITCHELL-at-rsbs.anu.edu.au
Date: Fri, 15 Jun 2001 13:27:33 +1000
Subject: Polymer Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a second hand copy of "Polymer Microscopy" by Sawyer & Grubb that they would like to sell?

Please let me know and we can talk $$$

Thanks

Meg

Meg Mitchell
EM Unit
RSBS
ANU
Meg.Mitchell-at-rsbs.anu.edu.au




From daemon Fri Jun 15 00:01:13 2001



From: Whunter-at-ushrl.ars.usda.gov ()
Date: Thu, 14 Jun 2001 23:57:42 -0500
Subject: Ask-A-Microscopist: protocol to embed insects

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(Whunter-at-ushrl.ars.usda.gov) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
June 14, 2001 at 07:58:06
---------------------------------------------------------------------------

Email: Whunter-at-ushrl.ars.usda.gov
Name: Wayne Hunter

Organization: USDA { ARS

Education: Graduate College

Location: Ft. Pierce, FL, USA

Question: I am looking for a protocol to embed insects in Paraplast,
and doing thick sections,
Thank you,
Sincerely,
Wayne Hunter
REsearch Entomologist

---------------------------------------------------------------------------


From daemon Fri Jun 15 00:01:48 2001



From: treese :      treese-at-marinebio.mbl.edu
Date: Fri, 15 Jun 2001 00:00:13 -0500
Subject: printers

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri Jun 15 02:10:36 2001



From: Emond W F de Roever :      ederoever-at-ONDEO-Nalco.com
Date: Fri, 15 Jun 2001 08:58:36 +0200
Subject: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haixin,

Growth of algae, bacteria etc may also be prevented/reduced by using
black, intransparant tubes instead of white transparant ones. They were
installed on my chiller for an ESEM 2020, after considerable problems.
With best regards, Emond de Roever, Ondeo Nalco Europe Technical Centre,
Oegstgeest, the Netherlands


"I have some problems with our EM chillers. The water in EM cooling
circulation is bad contaminated by algae, bacteria, maybe more
microorganisms. Does somebody here know some water cleaner for
chillers of
EM? Appreciate for the help."

Haixin Xu

Biological Sciences
University of Maryland, Baltimore County
Baltimore, Maryland











From daemon Fri Jun 15 03:51:18 2001



From: Dr Eric Lachowski :      e.lachowski-at-abdn.ac.uk
Date: Fri, 15 Jun 2001 09:47:01 +0100
Subject: chiller

Contents Retrieved from Microscopy Listserver Archives
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Haixin,
I've found that using distilled or de-ionised water in the chiller in
addition to the measures already suggested will also greatly reduce the
growth of microorganisms by denying them nutrients.

Regards,
Eric lachowski

------------
Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
Meston Walk
Old Aberdeen AB24 3UE
Scotland
Tel. +44 (0)1224 272934
Fax +44(0)1224 272921
e.lachowski-at-abdn.ac.uk



From daemon Fri Jun 15 07:32:27 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 15 Jun 2001 07:17:37 -0500
Subject: RE: printers

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I am using an Epson 980. I find it satisfactory for both B&W and color
prints and it is reasonably fast. It is also relatively inexpensive. Choice
of paper grade/type is very important in determining available print
quality.

Woody White
McDermott technology, Inc


} -----Original Message-----
} From: treese [mailto:treese-at-marinebio.mbl.edu]
} Sent: Friday, June 15, 2001 1:00 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: printers
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------
} ----------.
} }
} }
} We are going to replace our old Epson InkJet printer which produces
} nice B+W prints of micrographs, when it works. I haven't kept up
} with Epson vs HP for electron microscopists and wondered if a clear
} preference for one of these, or maybe another mfg or technology has
} emerged. We want B+W quality and reliability, and are willing to pay
} a bit more,and sacrifice speed and color quality...Thanks...Tom Reese
}


From daemon Fri Jun 15 07:32:27 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 15 Jun 2001 08:08:32 -0400
Subject: Re: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

I have seen a retrofit for Epson printers that looks quite interesting at

http://www.piezography.com/

I haven't actually seen it in action but it looks like it should be good
for B/W prints. The advertising samples on the web site look very
nice. Basically they replace the color cartridges with different densities
of black ink. By allowing gray inks, they reduce the amount of dithering
required. The product consists of the replacement cartridges and the
revised printer driver software.

Has anyone actually tried this approach?

Cheers,
Henk Colijn


At 12:00 AM 6/15/01 -0500, treese wrote:

} We are going to replace our old Epson InkJet printer which produces nice
} B+W prints of micrographs, when it works. I haven't kept up with Epson vs
} HP for electron microscopists and wondered if a clear preference for one
} of these, or maybe another mfg or technology has emerged. We want B+W
} quality and reliability, and are willing to pay a bit more,and sacrifice
} speed and color quality...Thanks...Tom Reese

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Fri Jun 15 08:11:15 2001



From: Roberson, Michael (M) :      MRoberson-at-dow.com
Date: Fri, 15 Jun 2001 09:06:51 -0400
Subject: W needles

Contents Retrieved from Microscopy Listserver Archives
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Does anyone happen to know in what issue of microscopy Today the
following information on sharpening W needles was found? (or how to make
them) If so please send me a note at, MRoberson-at-dow,com

I don't know about the current crop of FE cathodes, but in the past an
electrolytic cell was set up with the W needle as part of the circuit.
The needle was slowly dipped and removed several times from the NaOH
electrolyte. Essentially, it was the same technique as was (and is)
used to produce micomanipulator needles. I think the set-up was shown
and described well in a Microscopy Today article within the past 12 months.

Michael Roberson
(517)636-8656
SMX
Analytical Sciences
Midland, MI 48667



From daemon Fri Jun 15 08:43:56 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 15 Jun 2001 09:41:44 -0400
Subject: RE: Algae in water chiller

Contents Retrieved from Microscopy Listserver Archives
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The question of avoiding algal growth in water chillers is discussed
at length on p. 216 of my book 'Vacuum Methods in Electron
Microscopy' (see http://www.2spi.com/catalog/books/book48.html and
http://www.pup.princeton.edu/titles/6484.html)

One important way of reducing algal growth is to exclude light from
the system by keeping the reservior tank covered and by using opaque
tubing. If this is not sufficient then we have had great success
over many years by adding the algacide known as 'Chloramine-T' to the
water in the amount of about 0.25 grams per liter. This stuff is
available from most speciality chemical companies (Aldrich, Sigma,
Polysciences) under the chemical name of
N-chloro-p-toluensulphonamide. It is not highly soluble, and so is
not likely to produce deposits in the system. We have not had any
trouble with damage to any of our systems in which this material has
been used.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Fri Jun 15 08:56:43 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Fri, 15 Jun 2001 09:50:43 -0400
Subject: University service lab charges

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Jensen, Karen
Sent: Thursday, June 14, 2001 10:23 AM
To: 'Kathy Wolken'
Cc: 'microscopy-at-msa.microscopy.com'


Dear Kathy:

At the University of Rochester Electron Microscope Research Core, we charge
$75.00/hour with an Electron Microscopist running & photographing using our
Hitachi 7100 TEM, without the EM person, a trained researcher or grad
student must be trained on its use and the rate becomes $50.00/hour.

Our processing and preparation of tissue is at $75.00/hour and that only
counts the hands-on times. For instance, usually it could take 30 minutes
to do 1 micron sections and thin section the same block. We bill for
.5hr=$37.50 to cut one block, .25 hr=$18.75 if no screening 1 micron
sections are needed.

Most investigators have us embedd 2-6 blocks of tissue, etc. We cut a
representative block, review the 1 micron with them if necessary and then
proceed with the thin-sectioning. Staining is usually .25hr...etc, If the
investigator wanted more than one block cut and thin sectioned(3-5 grids),
they would all be stained together using a Hiroka staining kit(holds up to
40 grids) and therefore would only be charged the .25hr rate. However, the
methods and materials you use and speed in which they are performed can vary
from one lab to another.

We only billed $35,000 last year and that doesn't begin to cover salaries.
So the University of Rochester has to subsidize the EM Core. However, the
Core(s) is a big marketing tool for those researchers which are actively
being recruited. Many Molecular Science researchers utilize the EM Core for
ImmunoEM procedures. In fact, the majority of my work is performing pre-
and postembedding immunolabeling for EM. The average bill for this varies
from $300-1,000.

Hope this helps you.

Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642

-----Original Message-----
} From: Kathy Wolken [mailto:wolken.1-at-osu.edu]
Sent: Wednesday, June 13, 2001 9:15 AM
To: microscopy-at-sparc5.microscopy.com


I run a research based service lab at Ohio State University and I've been
asked to find out what other universities charge their internal customers
for the use of TEM, SEM, confocal microscopy and technical assistance. I'm
particularly interested in biological labs at large state universities, big
10 universities, and Ohio universities. Please respond directly to
me. Thanks.
Kathleen S. Wolken
Senior Electron Microscopist
Campus Microscopy and Imaging Facility (CMIF)
4029 Graves Hall
333 West 10th Avenue
Columbus, OH 43210-1239

Phone 614-292-9786
FAX 614-688-8742
WEB http://www.med.ohio-state.edu/cmif




From daemon Fri Jun 15 08:59:10 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Fri, 15 Jun 2001 09:58:56 -0400
Subject: Re: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you can purchase algicide from Fisher, VWR or other lab suppliers.




Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Haixin Xu {xu-at-gl.umbc.edu} 06/14 5:22 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Hi lister,

I have some problems with our EM chillers. The water in EM cooling
circulation is bad contaminated by algae, bacteria, maybe more
microorganisms. Does somebody here know some water cleaner for chillers of
EM? Appreciate for the help.

Haixin Xu

Biological Sciences
University of Maryland, Baltimore County
Baltimore, Maryland





From daemon Fri Jun 15 09:22:37 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 15 Jun 2001 10:01:26 -0400
Subject: W needles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't remember which issue of MT, but the following two paragraphs are three items that I sent to the Listserver recently describing sample preparation of emitters.

1)(Ni)
I'm trying to remember back to grad school, but I'm pretty sure that it was a 10%HCl solution in water. I used it for Ni FIM samples. I'll look it up in my dissertation and see if I have it. Hren's book or Bowkett and Smith's book on FIM had the reference for Ni. I do remember that the solution worked better after it was used some. It took on a greenish tinge. So what I started doing with a fresh batch, was taking a little crystalline NiCl (green powder) a putting just a bit into solution to get the color just right. I can't remember if I used ac of dc.

2)(W)
I used to prepare W FIM emitter tips with a 5% NaOH solution and I think the voltage was around 5 to 10 volts ac. When I used to electropolish, the FIM solutions and conditions were frequently similar to the jet polishing solutions.

3)(Fe & floating layer method)
You can use Field Ion Microscopy sample prep techniques to prepare very sharp needles of the wires. Basically, you can dip them in a beaker and electropolish them. The liquid/air interface will preferentially polish them. You need to move the interface up and down the sample by moving either the sample or liquid. If the wires are long enough, you can float electrolyte on top of CCl4 layer and you can get two samples when the bottom part drops off. Look up some of the standard electropolishing solutions and conditions. One is based on percholoric/butylcelsolve and the other on chromic acid/acetic acid -I think.


FEG note:
Someone asked recently about FEG tips. One problem with these tips that anyone off the streets would have is getting material that is oriented. Normal W wires have a [011] texture. The desired orientation for emitters in order for them to have a low work function and high brightness is either [112] or [113] (I can't remember). Other than that, I think anyone with experience making FIM samples could probably duplicate their construction within a short time, even Schottky design.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Roberson, Michael (M) [mailto:MRoberson-at-dow.com]
Sent: Friday, June 15, 2001 9:07 AM
To: 'Microscopy-at-MSA.Microscopy.Com'



Does anyone happen to know in what issue of microscopy Today the
following information on sharpening W needles was found? (or how to make
them) If so please send me a note at, MRoberson-at-dow,com

I don't know about the current crop of FE cathodes, but in the past an
electrolytic cell was set up with the W needle as part of the circuit.
The needle was slowly dipped and removed several times from the NaOH
electrolyte. Essentially, it was the same technique as was (and is)
used to produce micomanipulator needles. I think the set-up was shown
and described well in a Microscopy Today article within the past 12 months.

Michael Roberson
(517)636-8656
SMX
Analytical Sciences
Midland, MI 48667



From daemon Fri Jun 15 10:16:04 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 15 Jun 2001 08:09:38 -0700
Subject: Re: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I installed the Lyson grayscale inks in our Epson 850N. The results are
sometimes amazing, but always at least good.

Optimal results is greatly affected by paper type and quality, it seems more
so than printing color. You will also need to fiddle with the colorspace
gamma. Sorry I can't give more details, but I haven't had time to work with
it too much since most people here are printing color. I think it has a great
deal of promise and is worth checking out. The Lyson website has a number of
tips and print comparisons for optimizing results.

As far as choosing printers, find people who have used any particular model.
Epsons are slow to start printing. Out of the 4 epsons I work with (3 850N, 1
3000) and another 3 in a colleague's lab. at least 1, often 2, are misfeeding
paper, engaging in randoms acts of stubborness, dropping off the network,
etc. The 3000 is extremely touchy about how you load the paper, backing
sheets, etc., and regularly soils itself with ink. Epson drivers have
improved - sometimes, they even allow use to automatically scale to fit the
page at print time. However, 2 of the 850N printers hasve never failed,
always ready to go, so these problems probably reflect Epson QC issues..

Regards,
Glen

"Hendrik O. Colijn" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Tom,
}
} I have seen a retrofit for Epson printers that looks quite interesting at
}
} http://www.piezography.com/
}
} I haven't actually seen it in action but it looks like it should be good
} for B/W prints. The advertising samples on the web site look very
} nice. Basically they replace the color cartridges with different densities
} of black ink. By allowing gray inks, they reduce the amount of dithering
} required. The product consists of the replacement cartridges and the
} revised printer driver software.
}
} Has anyone actually tried this approach?
}
} Cheers,
} Henk Colijn
}
} At 12:00 AM 6/15/01 -0500, treese wrote:
}
} } We are going to replace our old Epson InkJet printer which produces nice
} } B+W prints of micrographs, when it works. I haven't kept up with Epson vs
} } HP for electron microscopists and wondered if a clear preference for one
} } of these, or maybe another mfg or technology has emerged. We want B+W
} } quality and reliability, and are willing to pay a bit more,and sacrifice
} } speed and color quality...Thanks...Tom Reese
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://web.ceof.ohio-state.edu
} Fools are pleased when they discover error.
} The wise are pleased when they discover truth.

--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************




From daemon Fri Jun 15 10:23:56 2001



From: Smartech :      smartech-at-javanet.com
Date: Fri, 15 Jun 2001 11:31:55 -0400
Subject: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have always used Epson and have never been disappointed with its best
performance, but I do have an occasional problem with banding. I am on my
third model, an Epson stylus 900, over the last 6 years. It is of the 1440
DPI Varity. Now they have 2880, although I am usually happy with 720DPI. I
get the impression that HP's asset is reliability. HP has not focused on
photo image quality as much as Epson, but both printers have surpassed the
photo-realistic hurdle a few years ago, not to say that they are as good as
film of' course. It would be interesting to buy both, do a side by side
comparison and send the one that fails back. Let me know what you find.

On a side note I have been playing around with wide format prints. There is
some good in printing larger hard copies and scanning at lower magnification
to get a better representation of the surface, but where the limits are I
don't know.

Don't write off speed too much. Speed usually only comes at the cost of
dollars, not quality with these inkjets. I like USB as well, may be faster.

Ric

-----Original Message-----
} From: treese [mailto:treese-at-marinebio.mbl.edu]
Sent: Friday, June 15, 2001 1:00 AM
To: Microscopy-at-sparc5.microscopy.com


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Jun 15 10:48:00 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 15 Jun 2001 08:40:34 -0700
Subject: Re: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Tom -

I've gotten quite passionate about marine invertebrate macrophotography as
a retirement project (see the URL below if you like such things). I have a
lot of prints on display in various local educational venues, so I've been
very concerned with print longevity - at a reasonable cost, since I buy the
equipment with my own $$$. I suggest that you look at
http://www.luminous-landscape.com/1280.htm
for a critical review of the Epson 1280. Epson's 6-color process is
supposed to give 25-year color prints & 100-year B&W (see
http://www.wilhelm-research.com/). I've just bought a closeout 1270, for
$250; 1440 dpi rather than 2880, but what a deal!

Caroline



Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Jun 15 11:23:44 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 15 Jun 2001 11:59:25 -0500
Subject: water chiller water

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for all your replies to my question regarding oil red O. Here is
my attempt to summarize those replies (and resulting discussions) starting
with the earliest reply. Please forgive the haphazard manner of
organization.

Jaclynn Lett, Research Assistant jlett-at-cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314.977.0257 fax: 314.977-0030
Jaclynn M. Lett, Research Assistant
jlett-at-cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314.977.0257 fax: 314.977.0030

Ms. Allan-Wojtas,

In response to your request regarding oil red O lipid staining, here are the
replies I received (along with some discussions as well). I have not had
time to thoroughly digest everything myself, so please forgive the delay
with which I replied and the lenghthy and unorganized manner of compiling
the replies (listed in the order in which they were received).

Jaclynn M. Lett, Research Assistant
jlett-at-cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314.977.0257 fax: 314.977.0030


----------------------------------------------------------------------------
---------------------------------------
} Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
} (Epon or Epon-Araldite)? If so, has this been done by staining en bloc or
} by staining the sections. Sections would range from 1-4 microns in
} thickness.

After the tissue is embedded, the solvents used (alcohol, propylene oxide)
will have dissolved most or all of the lipids so there will not be much to
stain. If you stain before embedding, dehydration will remove the lipid and
the stain.

} We would also consider tissues embedded in glycol methacrylate.

Forget it if you are using alcohol (or acetone) for dehydration. I don't
know
of any dehydrating agents that will not remove lipids. Perhaps freeze drying
would work, but you still need an embedding media that won't dissolve
lipids.
Even after osmium, some lipid is lost in dehydration.

} We'd like
} to avoid frozen sections because we'd prefer the higher level of detail
} possible with plastic.

So would a lot of us! This is why frozen sections are used for lipid
staining.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************
----------------------------------------------------------------------------
--------------------------------
The lipids will be removed by the solvents used in processing. In tissues
post fixed with osmium tetroxide BEFORE embedding in these plastics, the
osmium will stain lipids black.

Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)
----------------------------------------------------------------------------
-----------------------------------
The problem with trying to do lipid staining in epon or plastic embedded
tissue is that inorder to embed in epon or gma you usually have to process
in solvents which will dissolve the lipids not to mention that the plastic
itself is a solvent, at least GMA is somewhat.

Patsy Ruegg
----------------------------------------------------------------------------
------------------------------------
I don't know anyone who has been able to "successfully" stain enbloc or
sections from resins with oil red O, Sudan black or any of the other common
lipid stains because the solvents used in the processing are all designed to
remove fats and lipids. The only way I could get true accurate staining was
with cryo sections. I have tried so many methods/ resin schedules/ types of
resin and the same problems always occur, if there is enough lipid remaining
to stain, it doesn't give a true representation of location and quantity.
I'm sorry if this doesn't help, other than to perhaps save you some time and
effort.
As always, if you do happen to find someone who swears by their method, I
would love you to email me a copy of it.
Cheers,
Kerrie

Kerrie Holmes
Histology, Microscopy Research
Research Division, Peter MacCallum Cancer Institute
Locked Bag #1 A'Beckett St. East Melbourne 8006
Phone: 9656 1244 / 1242 Fax: 9656 1411
Email: k.holmes-at-pmci.unimelb.edu.au
----------------------------------------------------------------------------
-----------------------------------
I do alot of samples in GMA and have tried oil red O on a couple of
occasions with no luck. I would be very interested to know if anyone has
done this and using what method. Good luck.
Regards
Liz

Elizabeth Cox
Fisheries Biologist
Queensland Department of Primary Industries
Northern Fisheries Centre
PO Box 5396
Cairns, Queensland, Australia 4870
Fax: 07 4035 1401
Ph: 07 4035 0158
----------------------------------------------------------------------------
---------------------------------------
Has anyone used Oil Red O to stain lipids in tissues embedded in
plastics
(Epon or Epon-Araldite)? If so, has this been done by staining en
bloc or
by staining the sections. Sections would range from 1-4 microns in
thickness.



A couple of people so far have commented on how hard this would be, and our
experience agrees with theirs.
HOWEVER Jaclynn also said:



We would also consider tissues embedded in glycol methacrylate.
We'd like
to avoid frozen sections because we'd prefer the higher level of
detail
possible with plastic.



In THAT case things look better! Would you settle for Sudan black, rather
than Oil red staining of the lipid? If 'yes' then there is a method - which
does indeed show even tiny droplets of lipid very clearly. This was worked
out by the one-time king of GMA staining Peter Gerrits, and can be found in
J
Neurosci Methods, as follows:

Gerrits PO, Brekelmans-Bartels M, Mast L, 's-GrAavenmade EJ, Horobin
RW and Holstege G. (1992)..
Staining myelin and myelin-like degradation products in the spinal
cords of chronic experimental
allergic encephalomyelitis (Cr-EAE) rats using Sudan Black B staining
of glycol methacrylate-embedded
material.
J. Neuroscience Methods. 45, 99-105

Bye - Richard Horobin

Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin-at-aol.com
"What should we expect? Everything."
----------------------------------------------------------------------------
------------------------------------
Richard-

Whenever I've worked with those plastics, there has always been a clearing
stage through acetone. Since acetone would remove all non-bound fat, Oil
Red O would have nothing to go into.

When I've worked with these plastics, I also did a post-fixation in osmium
tetroxide, which does a very good job of staining fats and lipid (it turns
them black). Perhaps this would work for your purposes.

Joe

Joseph A. Saby, BA, HT(ASCP)
Drug Safety Evaluation
Pfizer Global Research and Development
2800 Plymouth Road
Ann Arbor, MI 48105
Phone: (734)-622-3631
FAX: (734)-622-3866
E-mail: joseph.saby-at-pfizer.com
----------------------------------------------------------------------------
--------------------------------------
Certain stains for light microscopy are not usable if you are trying
to stain for lipid and increase resolution of the sectioned tissue. One has
to adjust one's thinking and look only at how lipid can be preserved AND
processed into a plastic, which involves solvents such as alcohol during the
dehydration and infiltration steps.
If you are an experienced EM person, you have probably noticed that
lipid has been seen as a green color in your semithin secions. If you look
at Toluidine Blue stained semithin sections, lipid remains green, and the
nuclei and cytoplasm are blue. The resolution of epoxy and the green color
allows for subcellular identification of lipid.

HOWEVER, the preservation of lipid by osmium can be greatly enhanced
by p-phenylenediamine (use carefully, it can cause contact dermatitis and
asthma). Osmicate normal time, then start you dehyration procedure 25%,
50%,then put tissue in a 1.0% p-phenylenediamine in 70% ethanol for 15-25
minutes, then finish dehydratation procedure as normal, up to 100% ethanol,
into ethanol/propylene oxide, etc....Semithin (1-4u)sections without any
additional staining will show lipid by light microscopy. If needed,
counterstain nuclei with methylene blue stain or Toluidine Blue.

Glycol methacry. would not work, because none of the lipid will
remain, due to the alcohols used in processing. Only osmication, and
especially post- osmication treatment of tissue with p-phenylenediamine
perserves and stablizes lipid so it doesn't wash out during dehyration
steps.

Good Luck!

Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642
----------------------------------------------------------------------------
----------------------------------------------
Years ago I prepared a demonstration slide of testes for the Leydig cells
that came from a conventional EM block, Glut/Cacod and Osmium. 1 micron
section stained with Sudan black then Toluidine blue. Beautiful result but
it was only a one-off, although something I'll try again if needed.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855. Extn:6602.
Fax: 0141 330 2923
e-mail: ian.montgomery-at-bio.gla.ac.uk
----------------------------------------------------------------------------
----------------------------------
A question: is this really the case? Osmium binds across double-bonds
of lipids, which is why it shows and preserves membranes, but it
binds poorly or not all at to saturated lipids. So I wouldn't expect
OsO4 to show fatty deposits if the fats are saturated. Oil Red O and
Sudan Black, which more mix into the lipids and don't react with
them, would show fat deposits that OsO4 doesn't, and are better fat
stains.

Phil

} Agreed, there would be no need to do special fat stains if the tissue is
} processed with osmium. In fact, this can be done for paraffin embedding as
} well to show fat distribtion in some diseases with vastly better morphology
} and localization than frozen sections.
}
} Tim Morken
} CDC, Atlanta
----------------------------------------------------------------------------
---------------------------------------
Do you have any fixed, unprocessed tissues to work with?? If so, do
frozens on them instead of your blocks. This is what all the ORO protocols
that I have say to do, which makes sense when you consider that all
processing will subject the tissues to lipid solvents, thus all or most of
the lipids will have been removed and plastics are especially good lipid
solvents.

Connie McManus
----------------------------------------------------------------------------
----------------------------------------
The block was post fixed with osmium tetroxide/cacodylate then stained with
Sudan black. It was only a wee trial just to see what happened. At the time
I naively thought that the Sudan black must be binding to the osmium fixed
fat. It was only a once of on a single slide. I stained another slide with
Toluidine blue/Pyronine Y and it was just as lovely. Goodness knows what the
answer was. At the time I used to try all sorts of staining combinations on
semi-thin Glut/Osmium resin sections. Some were awful but others showed
promise, unfortunately time didn't permit further investigation.
The best staining for Glut/Osmium resin sections, in our hands was
Toluidine Blue/Pyronine Y (Ito & Winchester 1963 J. Cell Biol) and a
Polychrome technique (Pasyk, Bartok & Fabry 1989 Stain Technol 64 (3) 149.
Ian.

X-Sender: uvsgc-at-trex2.oscs.montana.edu
X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)


MicroListers,

This is a timely topic for me. We are getting a new chiller for our two EM's
and I'm reconsidering what to use in it. For over 20 years I've run them
with tap water and a dusting of dichlorophene fungicide on the surface of
the water in the tank, which powder slowly dissolves into the water. Have
had no problems at all with corrosion, plugged up lines (well, only ONCE,
when I'd failed to add fungicide after water change), so I'm inclined to
keep doing that, pending manufacturer's recommendations when I get the new
chiller.

However, other voices have advised using 10% propylene glycol (pure stuff,
NOT as in automobile anti-freeze) in de-ionized water.

Would like to revisit the pro's and con's of each method.

Thanks for your advice, in advance,

Gib Ahlstrand



} Haixin,
} I've found that using distilled or de-ionised water in the chiller in
} addition to the measures already suggested will also greatly reduce the
} growth of microorganisms by denying them nutrients.
}
} Regards,
} Eric lachowski

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Jun 15 12:19:56 2001



From: wft03-at-health.state.ny.us
Date: Fri, 15 Jun 2001 13:15:30 -0400
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







Just out of curiousity. . . how much X-radiation penetrates the rubber
stopper?

Marie

Dear Marie,
The short answer is, "Nearly all of it." The flux of x-rays depends
on such parameters as the location of the stopper with respect to things in
the column which can scatter the incident beam, produce brehmsstrahlung,
etc. The easiest thing to do is to take a counter and make the
measurement. For x-rays, the appropriate detector is an ionization
chamber, such as a hand-held QT-Pi; however, a Geiger counter can also be
used to give a relative measurement between the original set-up and that
with the stopper.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Jun 15 12:29:56 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Fri, 15 Jun 2001 13:23:08 -0400
Subject: RE: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Luminos http://www.lumijet.com/mono.htm also has quadtone inksets
for many Epson printers. With any of the quadtone type ink systems, you
will really need to dedicate the printer to printing only black and white.
It is impractical to switch between color and monochrome ink sets as
the printer needs to be flushed out before each switch.

I have seen some "fine art" photo samples printed with this ink
system and the results are stunning.

George

George Laing
National Graphic Supply
v:(518) 438-8411 X3109
f:(518) 438-0940
email: scisales-at-ngscorp.com
}
} Tom,
}
} I have seen a retrofit for Epson printers that looks quite interesting at
}
} http://www.piezography.com/
}
} I haven't actually seen it in action but it looks like it should be good
} for B/W prints. The advertising samples on the web site look very
} nice. Basically they replace the color cartridges with different
} densities of black ink. By allowing gray inks, they reduce the amount of
} dithering required. The product consists of the replacement cartridges
and the
} revised printer driver software.
}
} Has anyone actually tried this approach?
}
} Cheers,
} Henk Colijn



From daemon Fri Jun 15 12:53:11 2001



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 15 Jun 2001 12:46:54 -0500
Subject: RE: Algae in water chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That stuff that they use in waterbeds to avoid algae works wonders in these
chillers.



From daemon Fri Jun 15 13:09:13 2001



From: Smartech :      smartech-at-javanet.com
Date: Fri, 15 Jun 2001 14:16:58 -0400
Subject: Re: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I too have heard this tick from a company in long Island NY. I called, got
sample prints, looked good, but I could tell better with my own images. It
also might be cheaper since you can do continuous feed on some Epson
products and buy ink in bulk.

-----Original Message-----
} From: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
Sent: Friday, June 15, 2001 8:09 AM
To: treese; Microscopy-at-sparc5.microscopy.com


Tom,

I have seen a retrofit for Epson printers that looks quite interesting at

http://www.piezography.com/

I haven't actually seen it in action but it looks like it should be good
for B/W prints. The advertising samples on the web site look very
nice. Basically they replace the color cartridges with different densities
of black ink. By allowing gray inks, they reduce the amount of dithering
required. The product consists of the replacement cartridges and the
revised printer driver software.

Has anyone actually tried this approach?

Cheers,
Henk Colijn


At 12:00 AM 6/15/01 -0500, treese wrote:

} We are going to replace our old Epson InkJet printer which produces nice
} B+W prints of micrographs, when it works. I haven't kept up with Epson vs
} HP for electron microscopists and wondered if a clear preference for one
} of these, or maybe another mfg or technology has emerged. We want B+W
} quality and reliability, and are willing to pay a bit more,and sacrifice
} speed and color quality...Thanks...Tom Reese

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.





From daemon Fri Jun 15 13:38:28 2001



From: wft03-at-health.state.ny.us
Date: Fri, 15 Jun 2001 14:33:22 -0400
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Fortunately, the EDS port is to the rear of the column and pointing upward.

Dear Earl,
Fortunate for the user; however, an often-neglected aspect of
radiation shielding is that sufficiently energetic x-rays will penetrate
floors and ceilings as well as walls, so check with the folks upstairs if
you have a TEM--especially an IVEM or HVEM.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Jun 15 13:57:26 2001



From: wft03-at-health.state.ny.us
Date: Fri, 15 Jun 2001 14:53:23 -0400
Subject: Re: rubber stopper

Contents Retrieved from Microscopy Listserver Archives
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I'd love some feed-back from someone who knows radiation because I've
felt that applying TEM rules to SEMs is gross over-kill, especially with
so many color monitors around. How often do you have your decelerating
grid checked for proper operation? If it doesn't operate correctly,
your exposure could be very dangerous, given the time that one sits in
front of these things and their distance from your face.

If I'm way off base, I'd like to know and know why.

Dear Ken,
I think I qualify as someone who knows radiation, so here goes. Yes,
TEMs and SEMs are different, but the standards for stray radiation should
be the same. It is a lot easier to meet the standards with a low-voltage,
low-current machine, so the necessary shielding for a SEM would be less
difficult than for a TEM, but there should still be less than the specified
x-ray flux at the surface of the column. The Electron Microscopy Safety
Handbook (2nd Ed., 1994), p 49 gives a standard set by the Radiation
Control for Health and Safety Act of 1968 as 0.5 mR/hr at 5 cm from the
surface of the column. I am not aware of any update of this standard, but
if there is, the exposure allowed would surely be lower. A hand-held
ionization chamber can be used to measure the radiation from both the
microscope and the color monitors.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Jun 15 14:45:38 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Fri, 15 Jun 2001 15:23:19 -0500
Subject: W needles

Contents Retrieved from Microscopy Listserver Archives
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Hi Michael,
I believe that the article in Microscopy Today that you are looking for is
"Preparation And Use Of Needles and Micropipets For Handling Very Small
Particles" by Anna Teetsov of McCrone Research Institute. It is a 4-pager
that I am faxing to you.
I would be pleased to fax copies to anyone else on the list that would like
a copy.
Best to all,
Don Grimes, Microscopy Today

-----Original Message-----
} From: Roberson, Michael (M) [mailto:MRoberson-at-dow.com]
Sent: Friday, June 15, 2001 8:07 AM
To: 'Microscopy-at-MSA.Microscopy.Com'



Does anyone happen to know in what issue of microscopy Today the
following information on sharpening W needles was found? (or how to make
them) If so please send me a note at, MRoberson-at-dow,com

I don't know about the current crop of FE cathodes, but in the past an
electrolytic cell was set up with the W needle as part of the circuit.
The needle was slowly dipped and removed several times from the NaOH
electrolyte. Essentially, it was the same technique as was (and is)
used to produce micomanipulator needles. I think the set-up was shown
and described well in a Microscopy Today article within the past 12 months.

Michael Roberson
(517)636-8656
SMX
Analytical Sciences
Midland, MI 48667




From daemon Fri Jun 15 15:04:33 2001



From: wft03-at-health.state.ny.us
Date: Fri, 15 Jun 2001 16:00:21 -0400
Subject: Re: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






I have some problems with our EM chillers. The water in EM cooling
circulation is bad contaminated by algae, bacteria, maybe more
microorganisms. Does somebody here know some water cleaner for chillers of
EM? Appreciate for the help.

Dear Haixin,
We use dichlorophene to prevent the growth of micro-organisms, but you
may also need to remove those which are already in your system. The
appropriate cleaner will depend on what the components of the system are
made of. If you can disassemble and clean out the hoses, lenses, and
chiller separately, you would probably be able to get things cleaner, but
it could be a big effort. Cu++ is toxic to algae, and will not usually
damage metals, so you might want to circulate a solution of CuSO4, pH
adjusted to ~8 through the lenses if the lines are constricted within the
lenses. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Jun 15 16:26:04 2001



From: Marilyn Howton :      mhowton-at-hsc.wvu.edu
Date: Fri, 15 Jun 2001 17:19:29 -0400
Subject: RE:Little survey ..EMwise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } } } 700 { { { { {

Whew!
I am the only one in our EM lab. About 90 - 100 Kidney/surgical/autopsy specimens a year. About 30 or so research specimens a year. Not overworked here, but sure wish I had some backup, as vacations or sick days are a nightmare. I come in all hours, too.

Marilyn Howton
EM lab
Pathology Department
WVUniv. Hospitals



From daemon Fri Jun 15 16:50:51 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 15 Jun 2001 16:51:22 -0500
Subject: Sycon Instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a current email address for Sycon Instruments
(makers of film thickness monitors)?
Their web site is still up, but with a 1998 date, and the group
listed as maintaining the site is apparently defunct. No email
addresses are listed on the Sycon web site.

Thanks.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sat Jun 16 10:13:29 2001



From: RangeTS-at-aol.com
Date: Sat, 16 Jun 2001 10:04:49 -0500
Subject: cambridge 240

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

I have been looking for a set of schematics for cambridge 240. I am
wondering if anyone has any technical information for this instrument.

Best regards,
Ben Ghaffari
Email: Rangets-at-aol.com


From daemon Sat Jun 16 10:13:29 2001



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 16 Jun 2001 10:07:17 -0500
Subject: 7cm x 7cm em 4489 film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently got rid of 2 Zeiss EM 9s2 microscopes and am interested in
finding if there is anyone out there who is interested in buying 4489 EM
film for a Zeiss 9s2? I have aluminum cassettes, steel cassettes and ~10
boxes of film I wish to sell.

Please respond via my email address!

Cheers!
Ken
------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Dept. of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

email: tiekotte-at-up.edu


From daemon Sat Jun 16 11:08:36 2001



From: STANSMAN-at-aol.com
Date: Sat, 16 Jun 2001 13:28:41 EDT
Subject: Light Microscopy Imaging Contest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fortunately, in my lab, the administration was upstairs so not held in high
regard & the original question was for an SEM.

In addition, the rubber stopper solution was meant to be a temporary
situation.
Given that the maximum 25 - 30 KV X-rays need to penetrate several layers of
aluminum foil as well as concrete floor I doubt that it poses a health risk.
We had this situation at the last lab I worked & the Safety Department
measured the X-ray output and gave it a clean bill of health.
Not a good permanent situation but OK for the month or two.

Best Regards,

Earl




----- Original Message -----
} From: {"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Friday, June 15, 2001 11:33 AM


Hello Microscopists,

We all work very hard at producing beautiful and relevant microscope images.
Now is your chance to submit your finest images to the "Small World 2001"
contest. This is the 26th year for the famous contest that results in a
beautiful calendar showcasing the winners.

The closing date for submitting your images is June 29th, 2001. This
can now be accomplished easily and quickly via the
http://www.microscopyu.com/smallworld/ website.

There's still time to enter this famous optical photomicrography
competition which is open to both professional and amateur
microscopists; 35mm slide and/or digital entries of images taken with any
kind
of light microscope are accepted. Follow this link -

http://www.microscopyu.com/smallworld/

to learn more about the contest, see the fabulous prizes available, obtain
your "Small
World Screen Saver" of last years winners and view some of the stunning
images that have won in the past.

Also take time to browse the MicroscopyU host
site which has a wealth of exciting microscopy tutorials and resources.
Follow the
host link by typing this address into your browser -

http://www.microscopyu.com/


Good Luck and Best Regards,

Stan Schwartz
Manager, BioScience Dept.
Nikon Instruments Inc.
1300 Walt Whitman Rd.
Melville, NY 11747
Phone: 631-547-8529
Fax: 631-547-4033
email: sschwartz-at-nikon.net
www.nikonusa.com
Check out Nikon's Educational Website
www.microscopyU.com



From daemon Sat Jun 16 14:39:50 2001



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Sat, 16 Jun 2001 15:31:48 -0400
Subject: Tina's SEM images in NYTimes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




A nice plug for Tina's web pages from the NYTimes.

NYTimes 06-14-01


What You Can't See Microscopically

By SHELLY FREIERMAN

If you like to ponder questions
on the scale of how many Web
sites can fit on the head of a pin,
there is a fine collection of
electron microscope images to
examine at MicroAngela, an
online collection
(www.pbrc.hawaii.edu/bemf/microangela).
The Web site is maintained by
Tina M. Carvalho, the supervisor
at the Biological Electron
Microscope Facility at the
University of Hawaii in Manoa.

She has taken the images of tiny
bugs, bacteria, mold, parasites and
grains of sand, and colored them.

There is a description for each of the objects, which were collected
from captured insects; from samples of ocean life, like tubeworms
and coral; and even from mold growing on some Romano cheese in
Ms. Carvalho's refrigerator.


From daemon Sat Jun 16 15:37:41 2001



From: Dr. Edgar Voelkl :      mm2002-at-ornl.gov
Date: Sat, 16 Jun 2001 16:32:39 -0400
Subject: M&M 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow microscopists,

As indicated earlier (5-13-2001), I am soliciting suggestions for
symposia for the year 2002. So far we had very good response and I
am confident that the program for 2002 will be excellent. However,
before the program will be finalized, I would like to once more ask
for your idea for a great topic in any of the areas of

"Physical Sciences"
"Biological Sciences"
"Advances in Instrumentation and Techniques"

The deadline for suggestions is June 30th 2001. Please contact me
with your suggestions before June 30th under the following address:

mm2002-at-ornl.gov

Please forward this request also to your colleagues. Thank you for
your support.

With best regards,

Edgar Voelkl
Program Chair M&M 2002
--

___________________________

Dr. Edgar Voelkl
Program Chair M&M 2002

Oak Ridge National Laboratory
P.O. Box 2008
Bldg 4515
Oak Ridge, TN 37830-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913


From daemon Sun Jun 17 04:32:21 2001



From: Allen R. Sampson :      ars-at-sem.com
Date: Sun, 17 Jun 2001 04:26:49 -0500
Subject: RE: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've had Epson and Lexmark printers in the past, but can't say enough about
the HP Photosmart 1215 I've recently purchased. I've always had paper feed
problems with the Epson and Lexmark printers I've used, and noticed that
the HP I used on my home computer didn't have those problems. I decided to
try an HP printer for my business computer and chose this one. For both
color and grayscale images, I find it to be the closest to photographic
quality that I've tried (I used to do professional photography in the arts
field and did all of my own developing and printing).

The only caveat is that grayscale images should be printed in color mode -
black only prints in 600 x 600 resolution while color prints in 2400 x
1200.

This printer includes a separate 4 x 6 inch paper feed tray that is
excellent for small prints.

On Friday, June 15, 2001 12:00 AM, treese [SMTP:treese-at-marinebio.mbl.edu]
wrote:
} ------------------------------------------------------------------------
} }
} We are going to replace our old Epson InkJet printer which produces
} nice B+W prints of micrographs, when it works. I haven't kept up
} with Epson vs HP for electron microscopists and wondered if a clear
} preference for one of these, or maybe another mfg or technology has
} emerged. We want B+W quality and reliability, and are willing to pay
} a bit more,and sacrifice speed and color quality...Thanks...Tom Reese
}


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092



From daemon Mon Jun 18 00:58:47 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 18 Jun 2001 17:49:29 GMT+1200
Subject: RE: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} I've had Epson and Lexmark printers in the past, but can't say
} enough about the HP Photosmart 1215 I've recently purchased. I've
} always had paper feed problems with the Epson and Lexmark printers
} I've used, and noticed that the HP I used on my home computer didn't
} have those problems. I decided to try an HP printer for my business
} computer and chose this one.


Yeah, maybe, but boy, don't extend this to all HP printers.

I used to have at home an HP 400 inkjet, the cheapie, which kept on
giving paper-feed problems until I donated it to the repair shop
rather than fix it a second time, now, at work, I have a Laserjet 6L
that often feeds thru up to 10 sheets at a time!

I can't load up the input stack, have to insert each sheet to be
printed, one at a time.

It seems to me that the HP problem is that, in order to keep the
footprint small, the paper has to turn almost 180 degrees in a tight
circle.

The 6L has at the moment ANOTHER paper jam that I feel too
angry to fix, it's been jammed now for two days but I'd rather print
to the network printer and walk down the hall to retreive the
printout than pull it apart AGAIN.

If I had an outside window I'd be tempted to throw the *!-at-#$% printer
through it....................................


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Jun 18 08:11:50 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Mon, 18 Jun 2001 09:04:51 -0400 (EDT)
Subject: Re: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Lister,

Thank you all very much for the reply of my question. I think I figoured
out how to deal the problem.

Haixn Xu

Biological Sciences/UMBC
Baltimore Maryland


On Fri, 15 Jun 2001, Beauregard wrote:

} Hi,
}
} Chloramine-T can be used to kill or prevent the bacteria but it will cause
} corrosion problems with some instruments over time. This is especially
} true of
} stainless steel fittings where pit corrosion can become a very serious
} problem and it was for us. The manufacturer recommended using pure
} distilled water in our TEM. We didn't do what the service people said
} because we never had the problem with our other instruments in our wing of
} the building with C-T. We fixed our problem by using some manufacturer
} supplied brass fittings. We also switched to de-ionized water. It worked
} fine.
}
} Anyway, you could kill them with C-T, flush the system completely and then
} switch to pure distilled or de-ionized water. Check with your serviceman
} for the recommended material to use.
}
} After flushing, you can monitor the chloride level and the available
} chlorine to be sure you have removed the chloramine-T. You can use a
} swimming poll test kit for available chlorine and 0.1N silver nitrate
} solution for testing for chloride ions.
}
} After flushing and switching to pure distilled water our corrosion problem
} has not surfaced in over 8 years. We have about 10 instruments on our
} recirculator system.
}
} One further note. You can get a blue-green to green color in the water and
} inside the water lines. This can be copper ions or a green carbonate,
} like copper carbonate. Check any transparent PE or PP lines to see if they
} have a
} coating inside them of green copper carbonate. I did this on our Edwards
} 306 evaporator because it had transparent water lines on the DP. This
} color was thought to be algae but it was not algae alone. You can scrape
} off some of the coating and under a microscope apply hydrochloric acid.
} Look for any bubble generation to confirm the possible presence of copper
} carbonate. Of course, the bubbles could be another material like calcium
} carbonate but it would not ordinarily be greenish. Remove one of the lines
} if you can, add dilute HCl, wait for the line to 'clear' and run the acidic
} liquid into a test tube. If it's blue, you probably have copper ions and
} most likely copper carbonate deposits. We did. The copper can be
} confirmed by atomic absorption if necessary.
}
} Hope this helps.
}
} Paul Beauregard
} Senior Research Associate
} PPG Industries
} Monroeville, PA 15601
}
}
}
} At 05:22 PM 6/14/01 -0400, Haixin Xu wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Hi lister,
} }
} } I have some problems with our EM chillers. The water in EM cooling
} } circulation is bad contaminated by algae, bacteria, maybe more
} } microorganisms. Does somebody here know some water cleaner for chillers of
} } EM? Appreciate for the help.
} }
} } Haixin Xu
} }
} } Biological Sciences
} } University of Maryland, Baltimore County
} } Baltimore, Maryland
} }
} }
} }
} }
}
}



From daemon Mon Jun 18 08:59:08 2001



From: jshields-at-cb.uga.edu
Date: Mon, 18 Jun 2001 09:54:14 -0400
Subject: RE: W needles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Don,
I would appreciate a copy, if you wouldn't mind. I have a set-up of
my own, but it is quite the "homemade" variety.

Using this set-up, I have also produce fine blades by using
flattened W wire rather than the rods. Carefully dipping them in a
particular way produces a blade on one side and leaves the other
side as a support for strength. Works well when slicing nematodes.
John Shields
EM Lab
Univ. of GA, Athens


On 15 Jun 2001, at 15:23, Don Grimes wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of

} Hi Michael,
} I believe that the article in Microscopy Today that you are looking
} for is "Preparation And Use Of Needles and Micropipets For Handling
} Very Small Particles" by Anna Teetsov of McCrone Research Institute.
} It is a 4-pager that I am faxing to you. I would be pleased to fax
} copies to anyone else on the list that would like a copy. Best to all,
} Don Grimes, Microscopy Today



From daemon Mon Jun 18 12:12:49 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 18 Jun 2001 12:10:12 -0500
Subject: Re: Sycon Instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List,

Many thanks. I now have the contact information for Sycon Instruments.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Jun 18 12:43:00 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 18 Jun 2001 12:29:34 -0500
Subject: RE: water cleaner for EM chiller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use Chloramine-T from Alfa Aesar (was recommended by
FEI). 1 gramm per gallon.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: "wft03-at-health.state.ny.us"-at-sparc5.microscopy.com
} [mailto:"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com]
} Sent: Friday, June 15, 2001 3:00 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: water cleaner for EM chiller
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
}
}
}
} I have some problems with our EM chillers. The water in EM cooling
} circulation is bad contaminated by algae, bacteria, maybe more
} microorganisms. Does somebody here know some water cleaner
} for chillers of
} EM? Appreciate for the help.
}
} Dear Haixin,
} We use dichlorophene to prevent the growth of
} micro-organisms, but you
} may also need to remove those which are already in your system. The
} appropriate cleaner will depend on what the components of the
} system are
} made of. If you can disassemble and clean out the hoses, lenses, and
} chiller separately, you would probably be able to get things
} cleaner, but
} it could be a big effort. Cu++ is toxic to algae, and will
} not usually
} damage metals, so you might want to circulate a solution of CuSO4, pH
} adjusted to ~8 through the lenses if the lines are
} constricted within the
} lenses. Good luck.
} Yours,
}
} Bill Tivol
} Wadsworth Center
} Albany NY
} (518) 473-7399 WFT02-at-health.state.ny.us
}
}


From daemon Mon Jun 18 13:08:00 2001



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Mon, 18 Jun 2001 13:06:28 -0500
Subject: Anyone giving away an old Link EDS system?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We recently acquired an older Link EDS spectrometer and would like to use it
on an old ESEM. But we will need the pulse processor and whatever else to
run the detector. I have heard of people mixing brands and we have some
other EDS systems, but our old Link is presently installed on another scope.
Does anyone have an old Link giveaway or have any thoughts on compatibility
among the EDS systems?
Thanks for any thoughts or assistance.
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Mon Jun 18 13:17:53 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 18 Jun 2001 11:12:00 -0700 (PDT)
Subject: Re: Quantifying actin orientation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This is an update on a possible way of quantifying the orientation of
actin filaments within endothelial cells in culture.

I would like any comments on whether this seems reasonable or if there are
potential problems.

The fluorenscent labelled cells are captured and saved as grayscale
images. A highpass filter is used to enhance the filaments. The filaments
are thresholded and skeletonized. then the branch points are selected and
deleted. This creates many short linear segments whos "moment angle" or
orientation can be measured. There are about a 500 - 1000 measurements per
cell. The histogram of the angles have peaks cooresponding to the dominant
directions of filaments. I am hoping that control and experimental groups
of cells will have appropriatly different histograms.

Does this sound reasonable?

Bob Underwood
U of Washington



From daemon Mon Jun 18 13:30:37 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Mon, 18 Jun 2001 14:26:29 -0400 (EDT)
Subject: how to clean the dust on the mirror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listers,

I have a DURST S-45 enlarger that has not been used for a long time. The
mirror is heavily dusted. I want to clean it or want it cleaned. But I am
afraid to damage it if clean it not properly and I do not know where and
who can do the job for me. I am located in Baltimore. I would very much
appreciate your help.

Haixin Xu

Biological Sciences
University of Maryland, Baltimore County
Baltimore, MD 21250



From daemon Mon Jun 18 13:51:18 2001



From: Douglas C. Rennie :      drennie-at-unmc.edu
Date: Mon, 18 Jun 2001 13:57:45 -0500
Subject: TEM: Ultracut T

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a used Ultracut T that is in good/excellent condition.
Please contact Kathleen Greer or Lauren Simmerman if you know of one, -at-
Univ. of Nebraska Med. Cen.
402-559-7729 or
KathleenGreer-at-nhsnet.org

Thank You



From daemon Mon Jun 18 15:49:44 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 18 Jun 2001 13:37:24 -0700
Subject: RE: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paper jams mostly related to the paper quality (if printer itself is
OK). You may ask manufacturer which particular type of paper is better for
printer or just try different brands. For laser printers it should be
mentioned on the paper that this is for laser. Paper for laser and ink
-jets are opposite: glossy and "full-body" for laser and more porous (to
adsorb inks) for ink-jets. It also important to set correct
paper-thickness on the printer. They usually has thick-medium-thin
settings. It's mechanical switch, which presents on most models.

We do have some LaserJet B&W printer running for 5 years without any
serious problem with paper (heavy loaded, it serves as a network printer
for whole Department). We just bought a new Tektronix Color-laser last
year and it had jam problems near every day. We did change the paper and
it works very good now. Same - for ink-jets.

Sergey

At 05:49 PM 6/18/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Jun 18 16:29:20 2001



From: wft03-at-health.state.ny.us
Date: Mon, 18 Jun 2001 17:24:18 -0400
Subject: Re: how to clean the dust on the mirror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






I have a DURST S-45 enlarger that has not been used for a long time. The
mirror is heavily dusted. I want to clean it or want it cleaned. But I am
afraid to damage it if clean it not properly and I do not know where and
who can do the job for me. I am located in Baltimore. I would very much
appreciate your help.

Dear Haixin,
I would first remove the mirror (if possible), then gently blow as
much of the dust off it as possible. The concern, of course, is that some
of the dust may consist of mineral particles capable of scratching the
mirror. These should be somewhat denser and more easily removed in an air
stream than some of the other dust components. I would then rinse the
mirror with a stream of water and/or dunk it into a warm detergent
solution, then rinse carefully in distilled water followed by alcohol
and/or acetone. In none of these steps would I wipe the mirror even with a
very soft cloth. After this, I'd check to see how much dust is left. If
none, I'd clean the mirror with optic-wipe cloth, if little, I'd re-rinse
and do so until as much of the dust was removed as possible. I'd then
clean a small area with optic wipe cloth and inspect for scratches, if
none, then I'd clean the rest. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Jun 18 17:20:34 2001



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Mon, 18 Jun 2001 15:12:58 -0700
Subject: Cleaning Durst S45 front surfaced mirror

Contents Retrieved from Microscopy Listserver Archives
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Reply to Haixin Xu: Cleaning is done the same as it would be for a front surfaced telescope mirror as follows: 1) Gently let pure water (distilled or deionized) flow over the mirror. 2) dip several "Q-tip" swabs in a 1:100 diluted solution of Triton X 100. 3) allowing only the weight of the wet"Q-tips" to be the downward force gently pass them over the mirror and rinse with more pure water and allow to dry dust-free. If only traces of dust settle on the mirror you can blow them off gently with canned gas or a rubber bulb duster.



From daemon Tue Jun 19 02:12:12 2001



From: johnsond-at-ns.neiu.k12.pa.us ()
Date: Tue, 19 Jun 2001 01:55:56 -0500
Subject: Ask-A-Microscopist:Bacteria Viewing?

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Below is the result of your feedback form. It was submitted by
(johnsond-at-ns.neiu.k12.pa.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, June
18, 2001 at 13:44:52
---------------------------------------------------------------------------

Email: johnsond-at-ns.neiu.k12.pa.us
Name: Debbie Johnson

Organization: Valley View School District

Education: 6-8th Grade Middle School

Location: Archbald. PA USA

Question: What power of magnification would we need to be able to see
bacteria cells? They are not very clear using the standard classroom
scopes.(400x) Are there microscopes available for classroom use that
have more magnification?

---------------------------------------------------------------------------


From daemon Tue Jun 19 02:12:12 2001



From: Mendes, Maria :      mmendes-at-mtsinai.on.ca
Date: Tue, 19 Jun 2001 01:58:52 -0500
Subject: Morphometry Users Group?

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Hello everyone,

I was wondering if anyone out there had any information on a morphometry or
image analysis user group that I could get in contact with. Also does anyone
have an interest in a confocal microscope MRC 600 that we are going to
decommission. I would appreciate any information anyone could offer on any
of the above subjects.

Maria


From daemon Tue Jun 19 02:40:39 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 19 Jun 2001 08:52:01 +0100 (GMT Daylight Time)
Subject: Re: Anyone giving away an old Link EDS system?

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Hi Listers,
Is there anyone out there who can help with this request?
Please reply to plant-tc-at-tc.umn.edu.
Thanks,
Priscilla
*****************************************************************************

----- Original Message -----
} From: Abhay Shendye
To: plant-tc-at-tc.umn.edu
Sent: Sunday, June 17, 2001 12:05 PM


Hi Mike,

We have had this done the other way (Link have run another
maker's detector). They had to change the preamp onwards
and ensure that they got the pinout and bias voltage
to the crystal correct (obviously). We have also had
upgrades and they have always changed the preamp with the
pulse processor. What about contacting your local Oxford
Instruments rep. to see what they have from recent part
exchanges or upgrades?

Good luck,
Ron

} We recently acquired an older Link EDS spectrometer and would like to use it
} on an old ESEM. But we will need the pulse processor and whatever else to
} run the detector. I have heard of people mixing brands and we have some
} other EDS systems, but our old Link is presently installed on another scope.
} Does anyone have an old Link giveaway or have any thoughts on compatibility
} among the EDS systems?
} Thanks for any thoughts or assistance.
} Mike
} ********************************************************************
} Michael L. Boucher Sr. mboucher-at-tc.umn.edu
} Lab Manager Rm 55 Office Ph 612-624-6590
} I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
} University of MN Fax 612-625-5368
} 12 Shepherd Labs
} 100 Union Street S.E.
} Minneapolis, MN 55455 http://resolution.umn.edu
} ********************************************************************
}
}
}
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Jun 19 05:27:17 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 19 Jun 2001 03:20:37 -0700
Subject: Re: how to clean the dust on the mirror

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For EM guys it's not big deal to restore even scratched mirror. Only
limitation is the size of your vacuum evaporator's vacuum chamber.

1. remove old coating (very likely, it was aluminum, if so, use 1N NaON);
2 rinse in d-H2O and dry in clean place;
3. evaporate a new layer of Al (or Cr) using your vacuum evaporator.

The trick: never touch working surface before and after.

Sergey

P.S. Organic solvents may leave the traces even if they are very pure
(alcohol may have the trace of CuSO4 used for dehydratation or something
else). Last step in cleaning should be dd-H2O and drying in very clean
place. Who wear glasses half of life knows, that any organic solvents
leave the traces except detergent and then d-H2O. At least, it's my
experience.

At 05:24 PM 6/18/01 -0400, you wrote:
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From daemon Tue Jun 19 05:29:56 2001



From: Aarti Harle :      aarti_harle_em-at-usa.net
Date: 19 Jun 2001 04:25:37 MDT
Subject: Microstructure of wire

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Dear Listers,
I want to take the microstructure of 0.75mm wire, which is used by dentist.
Can anyone can guide for the sample preparation of wire for tem.

Arti Harle
Regional Sophisticated Instrumentation Center
Indian Institute of Technolgy-Bombay
Powai, Mumbai- 400076. India.
Phone No. 91-22-5767691 Extn. 7694
email: aartih-at-usa.net

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1


From daemon Tue Jun 19 08:33:12 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 19 Jun 2001 14:25:52 +0100 (GMT Daylight Time)
Subject: Re: Ask-A-Microscopist:Bacteria Viewing?

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If we assume your bacteria are 0.000001m long then if you
magnify them x400 they will appear 0.0004m long ie 0.4mm.

So if you would like them to appear, say 400mm long, you
would magnify by x40,000.

There are special microscopes called electron microscopes
which can give you this magnification. We look at
bacteria at x1,000 - x94,000. Your local university will
have some - why not check their web site?

Dave



On Tue, 19 Jun 2001 01:55:56 -0500
johnsond-at-ns.neiu.k12.pa.us wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form. It was submitted by
} (johnsond-at-ns.neiu.k12.pa.us) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, June
} 18, 2001 at 13:44:52
} ---------------------------------------------------------------------------
}
} Email: johnsond-at-ns.neiu.k12.pa.us
} Name: Debbie Johnson
}
} Organization: Valley View School District
}
} Education: 6-8th Grade Middle School
}
} Location: Archbald. PA USA
}
} Question: What power of magnification would we need to be able to see
} bacteria cells? They are not very clear using the standard classroom
} scopes.(400x) Are there microscopes available for classroom use that
} have more magnification?
}
} ---------------------------------------------------------------------------
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Jun 19 10:37:46 2001



From: Jim Nicolino :      JNicolino-at-xraydetectors.com
Date: Tue, 19 Jun 2001 11:30:31 -0400
Subject: Re: Anyone giving away an old Link EDS system?

Contents Retrieved from Microscopy Listserver Archives
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To answer the question of compatability.
The "older Link EDX" is probably an optical reset FET detector if it is
older than 1988. If it is a transistor reset FET ( PentaFet) then it
would have an attribute number on the Dewar label. Therefore when you
are looking for a compatable analyzer you will need to know which FET is
in the detector. The bigger problem is that Link used a software based
Restore signal to reset the FET therefore only a Link analyzer system
will operate the detector. The only present EDX company using a similar
software based Restore signal is Oxford ( Which bought Link Analytical
back in the early 1990's )
The pre-amp on the Link detector could be changed so that the Restore
signal is generated by the pre-amp. This would allow almost any analyzer
system to operate the detector but some compatability issues would still
remain.( Inhibit and Gain )
I hope this answers your question.
Kind Regards,
Jim Nicolino
X-Ray Optics / AAT
1816 St. Johns Bluff Road
Suite 305
Jacksonville, FL 32246
PH 904 646-3069
FAX 904 646-3131
E-mail JNicolino-at-xraydetectors.com

"Michael L. Boucher" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We recently acquired an older Link EDS spectrometer and would like to use it
} on an old ESEM. But we will need the pulse processor and whatever else to
} run the detector. I have heard of people mixing brands and we have some
} other EDS systems, but our old Link is presently installed on another scope.
} Does anyone have an old Link giveaway or have any thoughts on compatibility
} among the EDS systems?
} Thanks for any thoughts or assistance.
} Mike
} ********************************************************************
} Michael L. Boucher Sr. mboucher-at-tc.umn.edu
} Lab Manager Rm 55 Office Ph 612-624-6590
} I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
} University of MN Fax 612-625-5368
} 12 Shepherd Labs
} 100 Union Street S.E.
} Minneapolis, MN 55455 http://resolution.umn.edu
} ********************************************************************


From daemon Tue Jun 19 10:56:51 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 19 Jun 2001 08:53:58 -0700
Subject: Re: how to clean the dust on the mirror

Contents Retrieved from Microscopy Listserver Archives
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Hello Bill

Did you try Hexane to clean-up EM parts? Hexane, HPLC grade is very nice
for that. As a matter of fact, many organic solvents even very pure has
some additives, which is not listed. It includes the traces of compounds,
which were used during purification process. Very usual contamination -
sort of mineral oil, which comes when you store your stuff in the plastic
bottle (or it was stored somewhere before it comes to you in glass bottle)
- manufacturers use it in press-forms when they make plastic bottles. Any
plastic (even teflon) has some "plastifiers" to make plastic more
flexible. Those compounds does not chemically bonded to the plastic
matrix, so they erode slowly into stored solution. I had a deal with very
precise mirrors from analytical UV centrifuge. Those mirrors has precision
comparable with best telescope mirrors. I find, that many organic solvents
leave traces even if it never happen before on other surface. The mirror
surface, looks like so perfect and reflect easy very tiny imperfection. Of
coarse, I am talking about mirrors with reflecting coating up. As for
fingerprints - any organic solvent should work, I agree with you. Speaking
honestly, in the practical life my precautions may be not necessary. I was
talking in general, that scratches on the mirror is not a problem for
serious electron microscopist.

Thanks for your reply and have a great day.

Sergey.

At 10:20 AM 6/19/01 -0400, you wrote:




} P.S. Organic solvents may leave the traces even if they are very pure
} (alcohol may have the trace of CuSO4 used for dehydratation or something
} else). Last step in cleaning should be dd-H2O and drying in very clean
} place. Who wear glasses half of life knows, that any organic solvents
} leave the traces except detergent and then d-H2O. At least, it's my
} experience.
}
} Dear Sergey,
} d-H2O should leave no residue, but I've seen problems using it as the
} final rinse--probably because it takes a long time to dry, and our air may
} not be that clean. We use acetone as the final rinse on all the metal
} parts of the microscope that go in the vacuum, because it's easier to pump
} down if there is no residual H2O. We also use 95% EtOH to clean the glass
} plates in the Perkin Elmer microdensitometer--it is the best solvent for
} fingerprints--and we do not rinse with H2O afterward. I haven't seen any
} residue problems from either of these solvents.
} Yours,
} Bill



From daemon Tue Jun 19 12:07:12 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Tue, 19 Jun 2001 12:56:35 -0400
Subject: New confocal systems

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Hello fellow microscopists,
What are the latest advances in confocal microscopy systems? Any
with Dapi or Hoechst detection? What are the pros and cons of PMT vs
Camera acquisitions?
Aperture, Fixed slits or spinning discs? We would appreciate any input.

Thank you
Mike D.



From daemon Tue Jun 19 15:37:12 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 19 Jun 2001 13:27:43 -0700
Subject: Re: Ask-A-Microscopist:Bacteria Viewing?

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} ------------------------------------------------------------------------
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Debbie -

Yes, there are microscopes that will provide an excellent 1000x image. BUT
- they are quite a bit more sophisticated (and expensive) than the scope
that you are using, and they require careful setup to produce a good image.
It's possible that there is a microscopist who reads our ListServer who is
near Archibald who would be willing to visit your class to do a
demonstration. If you are interested, please write again.

You can find a lot of information about bacteria at the American Society
for Microbiology's educational website, http://www.asmusa.org/edusrc/


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Jun 19 19:43:38 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 19 Jun 2001 19:37:20 -0500
Subject: Poster Printing Grayscale Images

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I am trying (rather unsuccessfully) to print grayscale images on a
large format color printer. The printer does a superb job with color
images but generates truly unpredictable, bizarre renderings with
grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).

The printer has the option to print using black ink only but,
obviously, no color can be used elsewhere. I have made just about
every color management adjustment imaginable but have come to the
conclusion that it is not possible to print accurate colors and
grayscale simultaneously. Is that so?

I need professional help................. (in many ways).

Thank you.

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Tue Jun 19 23:25:04 2001



From: promotions9-at-websitepromotions.com
Date: Tue, 19 Jun 2001 19:02:07
Subject: DOMAIN NAMES $1.95 (REGISTER OR RENEWAL)

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Dear Web Professional:

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From daemon Wed Jun 20 04:27:41 2001



From: Martin Roe :      M.Roe-at-shannon.mluri.sari.ac.uk
Date: Tue, 19 Jun 2001 15:32:00 +0100
Subject: unsubscribe

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Please remove me from your mailing list





From daemon Wed Jun 20 05:23:20 2001



From: Aarti Harle :      aarti_harle_em-at-usa.net
Date: 20 Jun 2001 04:17:18 MDT
Subject: AFM

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I am working on a bacterial protein which are very unstable
polymerisation and depolymerization is within very short time.
I want to do the Atomic Force microscopy for these proteins
What is sample preparation for AFM
And how can I stabilise the protein.

Regards
Arti Harle


Ms. Arti Harle
Regional Sophisticated Instrumentation Center
Indian Institute of Technology-Bombay (IIT-B)
Powai, Mumbai - 400076.
India
Phone : 91-22-5767691 Extn 7694
91-22-5720109/ 5721039 (Resi)
E-mail : aartih-at-usa.net

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1


From daemon Wed Jun 20 06:55:01 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Wed, 20 Jun 2001 07:47:40 -0400
Subject: Printers and paper

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Hi, all,

This question has come up for us at a good time, with this ongoing discussion of printers.

We recently purchased an Epson 880 ink jet printer for our EM/LM lab, and are very happy with it. The black and white prints look excellent. We have tried a number of papers, and find that the Epson "Photo Quality Glossy Paper" and "Photo Paper" give the best results for our purposes.

We are wondering how to go about making our prints more permanent from these printers. If the prints have just been printed and aren't dry yet, or if they are dry and are in contact with any moisture, they smudge quite easily. Is there any coating - lamination or spray, or something else which we can use to protect the surface?

In connection with this, we have also found, especially when we have printed b/w prints, that if we leave them in a room with full spectrum fluorescent lights for a few days, that they begin to change colour, and start to take on a reddish tinge. The instructions that came with the paper say to not leave prints exposed to sunlight - but we're talking about room lights, here! Has anyone else found this happens to them, and is there any way of preventing/minimizing this effect without storing prints in a light tight bag????

Any help or suggestions would be much appreciated. As always, thanks for your help in advance.

Regards,

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Wed Jun 20 07:08:53 2001



From: Battjes, Kevin :      battjes-at-impactanalytical.com
Date: Wed, 20 Jun 2001 08:04:09 -0400
Subject: Job opening

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Listers,

Impact Analytical has an opening for a microscopist. Interested candidates
are invited to reply.


Job Description for Microscopist
IMPACT Analytical seeks an exceptional technical professional to become a
member of our Microscopy group. The candidate will be expected to provide
outstanding service to our customers on projects in the areas of microtomy,
SEM, TEM, and AFM. The candidate will be responsible for the development of
approaches to solve customer problems, as well as for projects from the time
that samples are received until the final report is written. This will
include communication with the customer with regards to clarifying project
goals and methods, conducting the analysis, updating the status, generating
the report, and following-up on customer requests. Experience in the
microscopy of plastics is a plus.
The ideal candidate will have at a minimum, a B.S. degree and 2 to 5 years
of relevant experience. The candidate should have a sense of urgency, strong
organizational skills, be detail oriented, and have very good laboratory
technique. The candidate should also have excellent oral, verbal, and
interpersonal skills, as well as familiarity with Microsoft based software.
Please respond by e-mail to Ms. Cherie Hutter {mailto:hutter-at-mmi.org} or by
mail to Ms. Cherie Hutter, Impact Analytical, Job Number 06-01, 1910 West
Saint Andrews Road, Midland, MI 48640 to arrive no later than 8 July, 2001.



Kevin Battjes
Impact Analytical
Michigan Molecular Institute
1910 W. St Andrews Road
Midland MI 48640



From daemon Wed Jun 20 07:24:23 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Wed, 20 Jun 2001 08:18:24 -0400 (EDT)
Subject: Re: Poster Printing Grayscale Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John...

We've had the same problem on our large format printer. I don't know what
the answer is either. I haven't gotten nearly as far as you have in terms
of troubleshooting, but my next step would be to call the manufacturer
(Epson, in our case). Did your manufacturer have any insight?

Angela V. Klaus

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Tue, 19 Jun 2001, John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am trying (rather unsuccessfully) to print grayscale images on a
} large format color printer. The printer does a superb job with color
} images but generates truly unpredictable, bizarre renderings with
} grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).
}
} The printer has the option to print using black ink only but,
} obviously, no color can be used elsewhere. I have made just about
} every color management adjustment imaginable but have come to the
} conclusion that it is not possible to print accurate colors and
} grayscale simultaneously. Is that so?
}
} I need professional help................. (in many ways).
}
} Thank you.
}
} JB
}
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ##############################################################
}
}



From daemon Wed Jun 20 08:15:08 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 20 Jun 2001 09:08:27 -0400
Subject: Re: Printers and paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

I too have noticed that the cyan ink that Epson uses is not very stable
even under fluorescent lights. However, enclosing a print in a
polyethylene sheet protector seems to improve the stability quite a bit.

Cheers,
Henk

At 07:47 AM 6/20/01 -0400, Paula Allan-Wojtas wrote:

} Hi, all,
}
} This question has come up for us at a good time, with this ongoing
} discussion of printers.
}
} We recently purchased an Epson 880 ink jet printer for our EM/LM lab, and
} are very happy with it. The black and white prints look excellent. We have
} tried a number of papers, and find that the Epson "Photo Quality Glossy
} Paper" and "Photo Paper" give the best results for our purposes.
}
} We are wondering how to go about making our prints more permanent from
} these printers. If the prints have just been printed and aren't dry yet,
} or if they are dry and are in contact with any moisture, they smudge quite
} easily. Is there any coating - lamination or spray, or something else
} which we can use to protect the surface?
}
} In connection with this, we have also found, especially when we have
} printed b/w prints, that if we leave them in a room with full spectrum
} fluorescent lights for a few days, that they begin to change colour, and
} start to take on a reddish tinge. The instructions that came with the
} paper say to not leave prints exposed to sunlight - but we're talking
} about room lights, here! Has anyone else found this happens to them, and
} is there any way of preventing/minimizing this effect without storing
} prints in a light tight bag????
}
} Any help or suggestions would be much appreciated. As always, thanks for
} your help in advance.
}
} Regards,
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Wed Jun 20 08:30:27 2001



From: hard-at-acsu.buffalo.edu
Date: Wed, 20 Jun 2001 09:25:33 -0500
Subject: LM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 10 - October 19, 2001

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: July 25, 2001

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual

research problems with the faculty.



From daemon Wed Jun 20 08:42:32 2001



From: swiding :      swiding-at-astro.temple.edu
Date: Wed, 20 Jun 2001 09:37:41 -0400
Subject: Critical point dryer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List,

I am trying to repair our Sorvall critical point dryer. I would like to
replace
the needle valve assemblies as they are starting to leak. Does anyone know if
parts are available for this system? If not, we will have to get it replaced.

Thanks for the help,

Steve Widing
Temple University



From daemon Wed Jun 20 09:02:28 2001



From: John Foust :      jfoust-at-threedee.com
Date: Wed, 20 Jun 2001 08:48:11 -0500
Subject: Re: Poster Printing Grayscale Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 07:37 PM 6/19/01 -0500, John J. Bozzola wrote:
} I am trying (rather unsuccessfully) to print grayscale images on a large format color printer. The printer does a superb job with color images but generates truly unpredictable, bizarre renderings with grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).
} The printer has the option to print using black ink only but, obviously, no color can be used elsewhere. I have made just about every color management adjustment imaginable but have come to the conclusion that it is not possible to print accurate colors and grayscale simultaneously. Is that so?

Which printer, which operating system, and which program is
doing the printing? When you say "color management" what
exactly do you mean that you tried?

Offhand, this sounds more like a function of the printer driver
and the program driving it. The program you're using to print
may only be capable of sending down grey or color at a time.
When it sends color, the grey gets treated (and color-dithered)
as color. In pure grey mode, no color dithering is introduced.

A more sophisticated printing program, such as a desktop
publishing program like Quark, may be capable of driving
the printer in a smarter fashion.

- John



From daemon Wed Jun 20 10:00:00 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Wed, 20 Jun 2001 09:55:03 -0500
Subject: Re: printers,PaperJam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie Simsr.sims-at-auckland.ac.nz6/18/01 12:49 PM

Ritchie,

Hey, I hear you, been there and done that! (HP - oi!). Finally, in
desperation I took a sheet of fine emery or sanding cloth to the rubber
rollers that move the paper through the printer - it worked! Try it, be
patient and do every roller you can get to. I've had to do this more than
once, but not often.I think the rollers pick up a "shine" from paper
coatings. Let me know what happens.

Chin up, man!

Gib

Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/


} }
} } I've had Epson and Lexmark printers in the past, but can't say
} } enough about the HP Photosmart 1215 I've recently purchased. I've
} } always had paper feed problems with the Epson and Lexmark printers
} } I've used, and noticed that the HP I used on my home computer didn't
} } have those problems. I decided to try an HP printer for my business
} } computer and chose this one.
}
}
} Yeah, maybe, but boy, don't extend this to all HP printers.
}
} I used to have at home an HP 400 inkjet, the cheapie, which kept on
} giving paper-feed problems until I donated it to the repair shop
} rather than fix it a second time, now, at work, I have a Laserjet 6L
} that often feeds thru up to 10 sheets at a time!
}
} I can't load up the input stack, have to insert each sheet to be
} printed, one at a time.
}
} It seems to me that the HP problem is that, in order to keep the
} footprint small, the paper has to turn almost 180 degrees in a tight
} circle.
}
} The 6L has at the moment ANOTHER paper jam that I feel too
} angry to fix, it's been jammed now for two days but I'd rather print
} to the network printer and walk down the hall to retreive the
} printout than pull it apart AGAIN.
}
} If I had an outside window I'd be tempted to throw the *!-at-#$% printer
} through it....................................
}
}
} cheers
}
} rtch
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}
}

--



From daemon Wed Jun 20 11:13:52 2001



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 20 Jun 2001 09:06:21 -0700 (PDT)
Subject: Re: Poster Printing Grayscale Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I am trying (rather unsuccessfully) to print grayscale images on a
} large format color printer. The printer does a superb job with color
} images but generates truly unpredictable, bizarre renderings with
} grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).
I think this is why color printing is CMYK and not just CMY. We typically
try not to allow the printer to print greyscale images with the color
setting on because of the off-tones. I think there really is no way to
achieve perfect grey with the color cartridges. I wonder if it would be
possible to trick the computer into printing only the greyscale images
with the black cartridge and everything else in color. The other
alternative would be to do a "two-pass" print with one print through on
the grey images, and then reprint again with color. This would be VERY
difficult on a poster printer, but a little easier with one of those
smaller feedthrough inkjet printers.
}
} The printer has the option to print using black ink only but,
} obviously, no color can be used elsewhere. I have made just about
} every color management adjustment imaginable but have come to the
} conclusion that it is not possible to print accurate colors and
} grayscale simultaneously. Is that so?
My only other suggestion is to continue with those color management
solutions--do you have all the color profiles configured from monitor to
printer? You've adjusted the file to CMYK gamut from RGB before printing?
The only other suggestion I can make(short of having the full colorimeter
and spectrophotometer calibration) is to make sure that the CMYK color
settings on the individual greyscale images are all manually adjusted to
absolute neutral.

Pauline Yu
Microscopist Technician
USDA-ARS-WRRC



From daemon Wed Jun 20 12:03:59 2001



From: woxberry-at-downstate.edu
Date: 6/20/01 9:08 AM
Subject: FWD: New Confocal user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



---------------------- Forward Header ----------------------

I am a long time Tem person starting a new job doing both TEM and
LSCM. Its very exciting learning LSCM but I need some suggestions for
ordering probes. Our lab has a Kr/Ar laser and will be doing
neuropathology research, mostly with fixed human brain samples, but
also tissue culture cells both live and fixed. A vibratome is in our
future. I was thinking of ordering alexa488nm-phalloiden, alexa568-
streptavidin, and syto60 from molecular probes to start getting used to
the scope and the lasersharp bio-rad software. The immunohistotech in
the lab uses an ABC dtection system with Dab. The first probe should
contrast the cytoskeleton well(stain F-actin), the second probe will
label any primary Ab, and the third will bind to DNA and contrast the
nucleus. Will using these three probes together be a good starting
point to becoming adept at labeling and producing high quality images
on the LSCM including optical sectioning and 3D (and 4D sometime in the
future)?
I welcome suggestions
Thank you

Bill




From daemon Wed Jun 20 13:29:00 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Wed, 20 Jun 2001 11:20:26 -0700
Subject: Poster Printing Grayscale Images

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John,

we used to have the same problem - the B&W images would come out pinkish. We
contacted HP Software Support about a year ago, and they pointed out that there
isn't any way to isolate a specific image and make it grayscale, leaving the
others in color. They suggested making the image a gray scale before importing
into the document. So far, when staff have copied the image and pasted it right
into the poster (a PowerPoint slide - enlarged to the size of the poster or half
the size of the poster) - it seems to work. However, there were some special
images that a few people had that didn't work. Good luck,

Alice.

Alice Dohnalkova
Dep. of Environmental Microbiology
Battelle, Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321




-----Original Message-----
} From: John J. Bozzola [SMTP:bozzola-at-siu.edu]
Sent: Tuesday, June 19, 2001 5:37 PM
To: Microscopy-at-sparc5.microscopy.com


I am trying (rather unsuccessfully) to print grayscale images on a
large format color printer. The printer does a superb job with color
images but generates truly unpredictable, bizarre renderings with
grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).

The printer has the option to print using black ink only but,
obviously, no color can be used elsewhere. I have made just about
every color management adjustment imaginable but have come to the
conclusion that it is not possible to print accurate colors and
grayscale simultaneously. Is that so?

I need professional help................. (in many ways).

Thank you.

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################

-----Original Message-----
} From: John J. Bozzola [SMTP:bozzola-at-siu.edu]
Sent: Tuesday, June 19, 2001 5:37 PM
To: Microscopy-at-sparc5.microscopy.com


I am trying (rather unsuccessfully) to print grayscale images on a
large format color printer. The printer does a superb job with color
images but generates truly unpredictable, bizarre renderings with
grayscale (SEM, TEM) images (greenish/brownish/pinkish tones).

The printer has the option to print using black ink only but,
obviously, no color can be used elsewhere. I have made just about
every color management adjustment imaginable but have come to the
conclusion that it is not possible to print accurate colors and
grayscale simultaneously. Is that so?

I need professional help................. (in many ways).

Thank you.

JB

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Jun 20 14:34:45 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Wed, 20 Jun 2001 15:26:43 -0400
Subject: RE: Printers and paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Luminos http://www.lumijet.com has a spray called
"Imagesheild" to protect prints. $18/ can. Quoting their website:
"LUMIJET IMAGESHIELD will become your favorite print protector.
It’s a convenient ozone-friendly low-odor aerosol spray that significantly
improves wet-fastness. In addition to protecting delicate inkjet images
from moisture, it also shields images from fingerprints and harmful UV rays.
This unique product will not only help extend useful print life,
it is totally transparent; preserving the original print surface
characteristics. From dead matte to glossy, or from canvas to silver foil,
you’ll never know it’s there."

} We are wondering how to go about making our prints more permanent
} from these printers. If the prints have just been printed and
} aren't dry yet, or if they are dry and are in contact with any
} moisture, they smudge quite easily. Is there any coating -
} lamination or spray, or something else which we can use to
} protect the surface?

There were recently (last 6-8 months) some issues with color shift
on some Epson papers. Epson determined the shift was from high
concentrations of ozone, and not due to exposure to light. Epson
recommends Heavyweight Matte or Epson Photo paper for maximum
lightfastness.


George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com
}
} In connection with this, we have also found, especially when we
} have printed b/w prints, that if we leave them in a room with
} full spectrum fluorescent lights for a few days, that they begin
} to change colour, and start to take on a reddish tinge. The
} instructions that came with the paper say to not leave prints
} exposed to sunlight - but we're talking about room lights, here!
} Has anyone else found this happens to them, and is there any way
} of preventing/minimizing this effect without storing prints in a
} light tight bag????
}



From daemon Wed Jun 20 16:31:11 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 20 Jun 2001 16:11:01 -0500
Subject: RE: Printers and paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Epson had a magenta ink fade problem. I have "heard" that it has been
improved. Anecdotal reading suggests the paper chemistry vs. ink can have a
bearing on colorfastness. Prints I have made in the last year or so on
Eposn "photo quality inkjet paper" and Kodak "Picture paper" have held up
reasonably well. I have also used Krylon clear acrylic spray to "fix" some
prints. ...Not bad for moisture, insufficient data about help with fade.
Use many light coats. If you really wet the surface, the paper can become
translucent.

WoodyWhite
McDermott Technology, Inc

{SNIP}
} } In connection with this, we have also found, especially when we
} } have printed b/w prints, that if we leave them in a room with
} } full spectrum fluorescent lights for a few days, that they begin
} } to change colour, and start to take on a reddish tinge. The
} } instructions that came with the paper say to not leave prints
} } exposed to sunlight - but we're talking about room lights, here!
} } Has anyone else found this happens to them, and is there any way
} } of preventing/minimizing this effect without storing prints in a
} } light tight bag????
} }
}
}


From daemon Wed Jun 20 19:09:42 2001



From: aiace_99-at-usa.net ()
Date: Wed, 20 Jun 2001 19:06:50 -0500
Subject: Ask-A-Microscopist:View cells coming from a dissected tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(aiace_99-at-usa.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
June 20, 2001 at 10:45:27
---------------------------------------------------------------------------

Email: aiace_99-at-usa.net
Name: Massimo

Organization: None

Education: Graduate College

Location: Italy

Question: I am writing to enquire about a technical problem.
I'd like to see with my microscope some cells coming from a dissected
tissue, for instance a vegetable material.
So, if I have such small tips into a drop of water and I want to
fix, stain and mount in Balsam, how can I perform this operation
without to loose that material from the slide?
Could you give me some information or a reference where I could find
the correct protocol?
Thank you
Massimo.


---------------------------------------------------------------------------


From daemon Wed Jun 20 19:09:42 2001



From: woxberry-at-downstate.edu
Date: Wed, 20 Jun 2001 19:04:41 -0500
Subject: New Confocal user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am a long time Tem person starting a new job doing both TEM and
LSCM. Its very exciting learning LSCM but I need some suggestions for
ordering probes. Our lab has a Kr/Ar laser and will be doing
neuropathology research, mostly with fixed human brain samples, but
also tissue culture cells both live and fixed. A vibratome is in our
future. I was thinking of ordering alexa488nm-phalloiden, alexa568-
streptavidin, and syto60 from molecular probes to start getting used to
the scope and the lasersharp bio-rad software. The immunohistotech in
the lab uses an ABC dtection system with Dab. The first probe should
contrast the cytoskeleton well(stain F-actin), the second probe will
label any primary Ab, and the third will bind to DNA and contrast the
nucleus. Will using these three probes together be a good starting
point to becoming adept at labeling and producing high quality images
on the LSCM including optical sectioning and 3D (and 4D sometime in the
future)?
I welcome suggestions
Thank you

Bill


From daemon Thu Jun 21 05:36:33 2001



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Thu, 21 Jun 2001 05:42:13 -0500
Subject: does anyone have a resolve workstation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


if so, how well is it liked?

http://www.resolve3d.com/in_the_news_resview.html
Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find
the cure for cancer and other diseases. There will always be a need for
the trained clinician (MD/RN) but, advanced diagnostic and treatment option
selection has become gene based, has moved from the physician's practice to
the computerized cell and molecular biology laboratory, and appropriate
treatment options should now be based on the personal biology of the
patient.


From daemon Thu Jun 21 07:27:39 2001



From: Damian :      neuberger1234-at-home.com
Date: Thu, 21 Jun 2001 07:22:14 -0500
Subject: BioRad Confocal MRC1000 Info needed

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
I have a BioRad Confocal MRC1000 on a Nikon Optiphot (upright scope)
and would like to use it on an inverted microscope but the only thing
I have is an older Diaphot with the 80/20 prism. Of course this is
unacceptable as there is no shutter to prevent someone from
inadvertently looking into the eyepieces while scanning.
Does anyone know if and how the sliders on the Diaphot can be
modified so as to serve as a shutter, i.e. in one position the
scanner input is blocked to allow viewing and in another it is
directed only to the objective/specimen? I assume it would mean a
modification to the internal prisms. Has anyone done this? Source for
parts? Procedure? Does anyone do this as a business? I called my
local Nikon dealer and was told that the 80/20 can not be converted.
Would I be better off to by a new inverted scope? Issues: cost, new
infinity corrected optics.
What are the issues with this changeover if you have done this.
If someone who is on the confocal list could forward this, I would
appreciate it. I have not been able to sign on since I changed my
email address.
Thanks so much for advice and help.
Damian Neuberger, PhD
Senior Research Scientist
Baxter Healthcare Corp.
email: damian_neuberger-at-baxter.com
Fax: 847/270-5897Phone: 847/270-5888


From daemon Thu Jun 21 07:27:39 2001



From: Damian :      neuberger1234-at-home.com
Date: Thu, 21 Jun 2001 07:22:37 -0500
Subject: Printing Gray scale on a deskjet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Along this thread, when we print B&W images on a HP deskjet, we set
printer properties to Grayscale, yet when we examine the image with a
hand lens, we can see color dots. Why? (perhaps I missed the answer
in a previous recent message, sorry). Damian


From daemon Thu Jun 21 07:46:48 2001



From: Randi Olsen :      randio-at-fagmed.uit.no
Date: Thu, 21 Jun 2001 14:42:14 +0200
Subject: HISTOLOGICAL SPECIMENS, SEMINAR BOXES

Contents Retrieved from Microscopy Listserver Archives
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I got a question from one of the Professors in Pathology here in Tromso,
and she wants to know if there are anyone selling seminar boxes with
specimens
(Light microsopy)from human pathology/anatomy?

Best regards
Randi Olsen
Department of Electron Microscopy
Faculty of Medicine
University of Tromso
N-9037 TROMSO
Norway



From daemon Thu Jun 21 08:19:01 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 21 Jun 2001 09:13:52 -0400
Subject: Critical point dryer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

I'm not certain but your Sorvall unit may be similar in design to the
Polaron CPD units that we service and sell in the U.S. If you could send us
a digital photograph and/or copies of any information on the unit that may
still be around, we will be happy to do some research to see if we can help
you.

Please feel free to contact me off line at mnesta-at-ebsciences.com or by phone
at (800) 992-9037.

Mike

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
www.ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: swiding [mailto:swiding-at-astro.temple.edu]
Sent: Wednesday, June 20, 2001 9:38 AM
To: microscopy-at-sparc5.microscopy.com


Hello List,

I am trying to repair our Sorvall critical point dryer. I would like to
replace
the needle valve assemblies as they are starting to leak. Does anyone know
if
parts are available for this system? If not, we will have to get it
replaced.

Thanks for the help,

Steve Widing
Temple University





From daemon Thu Jun 21 08:25:36 2001



From: David_Bell-at-millipore.com
Date: Thu, 21 Jun 2001 09:26:55 -0400
Subject: Re: does anyone have a resolve workstation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Sterling,

We just had a demo of their system from the local sales person, and I must
say I was greatly impressed. The company is very young and full of some
great ideas. The sales crew seems eager to work with potential customers.
The demo was good enough for the powers that be here to approve some "proof
of concept" work with some of our own samples. The software is simply
amazing in its potential. It seems to have a very user friendly, and
intuitive interface that is quite powerful. Obviously the biggest
limitation is resolution. Despite the claims of "high resolution", they
are limited to typical light microscopy resolutions of about 0.2um/voxel.
Another limitation is your material. If you are talking about biological
samples, there is no problem, but currently certain materials do present a
problem for them.

If you have more specific questions about what I saw at the demo, please
feel free to contact me.

Sincerely,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108





sterling
stoudenmire To: MICROSCOPY-at-sparc5.microscopy.com
{sstouden-at-the cc:
links.com} Subject: does anyone have a resolve workstation

06/21/2001
06:42 AM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


if so, how well is it liked?

http://www.resolve3d.com/in_the_news_resview.html
Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find
the cure for cancer and other diseases. There will always be a need for
the trained clinician (MD/RN) but, advanced diagnostic and treatment option
selection has become gene based, has moved from the physician's practice to
the computerized cell and molecular biology laboratory, and appropriate
treatment options should now be based on the personal biology of the
patient.






From daemon Thu Jun 21 08:39:41 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 21 Jun 2001 09:34:10 -0400
Subject: Critical point dryer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

I'm not certain but your Sorvall unit may be similar in design to the
Polaron CPD units that we service and sell in the U.S. If you could send us
a digital photograph and/or copies of any information on the unit that may
still be around, we will be happy to do some research to see if we can help
you.

Please feel free to contact me off line at mnesta-at-ebsciences.com or by phone
at (800) 992-9037.

Mike

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
www.ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: swiding [mailto:swiding-at-astro.temple.edu]
Sent: Wednesday, June 20, 2001 9:38 AM
To: microscopy-at-sparc5.microscopy.com


Hello List,

I am trying to repair our Sorvall critical point dryer. I would like to
replace
the needle valve assemblies as they are starting to leak. Does anyone know
if
parts are available for this system? If not, we will have to get it
replaced.

Thanks for the help,

Steve Widing
Temple University







From daemon Thu Jun 21 08:41:59 2001



From: Julie Piraino :      piraino-at-sms.si.edu
Date: Thu, 21 Jun 2001 08:39:48 -0500
Subject: service for old microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Boeckler/Ventana/RMC/Dupont-Sorvall no longer provide on-site service
for old MT2-B microtome, and no support for our MT-5000 microtome.
Does anyone know of a good, independent service provider for these
instruments in the SE United States?



Julie Piraino
Laboratory Manager
Smithsonian Maine Station
701 Seaway Drive
Fort Pierce, FL 34949
561-465-6630 x143
fax 561-461-8154


From daemon Thu Jun 21 09:48:55 2001



From: Kathy Wolken :      wolken.1-at-osu.edu
Date: Thu, 21 Jun 2001 10:53:45 -0400
Subject: NIH Image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm sending this request for a colleague.

I am using the threshold feature of NIH Image to analyze images of cell
cultures of individual neurons with processes. We are attempting to
quantitate the number of processes in each culture. However, when the
threshold function is applied, if a polygon is formed by the processes, NIH
Image fills in the space between the "lines" (coverts all the pixels to
black = "above threshold"). Since we are trying to measure the number of
pixels above threshold, the filled space is counted as part of the number,
thus preventing an accurate measurement.

Is there a way to stop the "fill" from occurring?

Thanks.

Paul Madtes Jr.
pmadtes-at-mvnc.edu
Kathleen S. Wolken
Senior Electron Microscopist
Campus Microscopy and Imaging Facility (CMIF)
4029 Graves Hall
333 West 10th Avenue
Columbus, OH 43210-1239

Phone 614-292-9786
FAX 614-688-8742
WEB http://www.med.ohio-state.edu/cmif




From daemon Thu Jun 21 10:37:03 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 21 Jun 2001 12:12:31 -0400
Subject: biofilms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Does anyone have experience in isolating biofilms from
sediment prior to fixation. I thinking of doing a pretreatment with
Tween-20 but would appreciate suggestions from anyone who has
prepared films for imaging in the LM and SEM.
Rosemary
--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Thu Jun 21 10:40:19 2001



From: craig klotz :      cklotz-at-mcw.edu
Date: Thu, 21 Jun 2001 10:34:43 -0500
Subject: Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone who uses a MT-6000, Ultracut E, or NOVA ultramicrotome please
comment on reliability, ease-of -use, lighting, etc. for those
machines. Our lab is going to purchase a used machine in July.

TIA,
Craig



From daemon Thu Jun 21 10:52:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 21 Jun 2001 11:46:20 -0400
Subject: RE: New Confocal user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I found that the learning curve was greatly improved by starting
with another Molecular Probe product. I acquired the 6um FocalCheck beads
(I got F-14807)(Search MP for document MP 07234) that fit our system - they
should be fairly generic. I have made up my own slides. You will probably
want one with three colors. The idea is to determine through experience the
relationship between Mag Power and Optical Section Thickness. The problem
in CSLM is not the x-y plane, it is learning how to get the z-axis right,
and how to balance the channels. Choosing the other probes is a question
that can only be answered by knowing as much as possible about the spectral
characteristics of the excitation and emission filters in your system so
that you can minimize crosstalk. NOTE: There is a paucity of information
about filters that forces us to be empiric in setting up experiments, at
least that's been my experience so far. I keep on asking for the
information from the system vendor.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: "woxberry-at-downstate.edu"-at-sparc5.microscopy.com
} Sent: Wednesday, June 20, 2001 8:04 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: New Confocal user
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am a long time Tem person starting a new job doing both TEM and
} LSCM. Its very exciting learning LSCM but I need some suggestions for
} ordering probes. Our lab has a Kr/Ar laser and will be doing
} neuropathology research, mostly with fixed human brain samples, but
} also tissue culture cells both live and fixed. A vibratome is in our
} future. I was thinking of ordering alexa488nm-phalloiden, alexa568-
} streptavidin, and syto60 from molecular probes to start getting used to
} the scope and the lasersharp bio-rad software. The immunohistotech in
} the lab uses an ABC dtection system with Dab. The first probe should
} contrast the cytoskeleton well(stain F-actin), the second probe will
} label any primary Ab, and the third will bind to DNA and contrast the
} nucleus. Will using these three probes together be a good starting
} point to becoming adept at labeling and producing high quality images
} on the LSCM including optical sectioning and 3D (and 4D sometime in the
} future)?
} I welcome suggestions
} Thank you
}
} Bill
}
}


From daemon Thu Jun 21 10:59:15 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Thu, 21 Jun 2001 08:54:10 -0700
Subject: SEM: Recommendations for BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,
I have a Jeol 5900 SEM, and I would like to purchase a BSE detector for
it. Does anyone in the group have any suggestions/recommendations/warnings? Is
it pretty straight forward to purchase a third-party detector and get it to work
with our microscope, or is it best to go through the vendor? Thank you for any
input that you can provide.


Brad Johnson
Pacific Northwest National Lab
P.O. Box 99, K6-24
Richland, WA 99352
voice: 509-372-1635
fax: 509-376-3108

Bradley.Johnson-at-pnl.gov


From daemon Thu Jun 21 11:13:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 21 Jun 2001 12:08:08 -0400
Subject: RE: New Confocal user

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I found that the learning curve was greatly improved by starting
with another Molecular Probe product. I acquired the 6um FocalCheck beads
(I got F-14807)(Search MP for document MP 07234) that fit our system - they
should be fairly generic. I have made up my own slides. You will probably
want one with three colors. The idea is to determine through experience the
relationship between Mag Power and Optical Section Thickness. The problem
in CSLM is not the x-y plane, it is learning how to get the z-axis right,
and how to balance the channels. Choosing the other probes is a question
that can only be answered by knowing as much as possible about the spectral
characteristics of the excitation and emission filters in your system so
that you can minimize crosstalk. NOTE: There is a paucity of information
about filters that forces us to be empiric in setting up experiments, at
least that's been my experience so far. I keep on asking for the
information from the system vendor.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: "woxberry-at-downstate.edu"-at-sparc5.microscopy.com
} Sent: Wednesday, June 20, 2001 8:04 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: New Confocal user
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am a long time Tem person starting a new job doing both TEM and
} LSCM. Its very exciting learning LSCM but I need some suggestions for
} ordering probes. Our lab has a Kr/Ar laser and will be doing
} neuropathology research, mostly with fixed human brain samples, but
} also tissue culture cells both live and fixed. A vibratome is in our
} future. I was thinking of ordering alexa488nm-phalloiden, alexa568-
} streptavidin, and syto60 from molecular probes to start getting used to
} the scope and the lasersharp bio-rad software. The immunohistotech in
} the lab uses an ABC dtection system with Dab. The first probe should
} contrast the cytoskeleton well(stain F-actin), the second probe will
} label any primary Ab, and the third will bind to DNA and contrast the
} nucleus. Will using these three probes together be a good starting
} point to becoming adept at labeling and producing high quality images
} on the LSCM including optical sectioning and 3D (and 4D sometime in the
} future)?
} I welcome suggestions
} Thank you
}
} Bill
}
}


From daemon Thu Jun 21 12:03:36 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Thu, 21 Jun 2001 09:51:50 -0700
Subject: TEM-Ultrathin film grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear colleagues,

I work with ultrasmall nanosized particles, as small as 2 nm, with much
lower contrast than metal particles such as gold and silver. These
ultrasmall particles don't show up very well on TEM, even with optimal TEM
settings. I thought what would help to increase the contrast dramatically
would be to use a TEM grid that has very low background signal. I have tried
grids with ultrathin carbon film (about 5 nm thick) but the background
signal is still too high for these particles to show up clearly. Holey
carbon grids is an option except they don't give me enough particles for
particle sizing. Any suggestions would be very much appreciated.


Regards,

Thearith



From daemon Thu Jun 21 12:26:01 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 21 Jun 2001 13:21:26 -0400
Subject: RE: Printers and paper - Sources of Inksets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a recent issue of "PEI", May, 2001 (http://www.peimag.com) the following
sampling of Inkset Resources was offered in an article entitled: "Inksets
for Desktop Inkjet Printers", by Theano Nikitas.

The point of the article was the difference between pigment and dye
inks, and those that are specially formulated for archival prints.

I am only using those that are not Printer Manufacturers

1. Cone Editions Press: http://www.inkjetmall.com

2. Luminos Photo Corp: http://www.luminos.com

3. Lyson USA Inc.: http://www.lysonusa.com

4. MIS Associates Inc: http://inksupply.com

5. MediaStreet.com: http://mediastreet.com

6. Xtreme gamut: http://xtremegamut.com

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.

} ----------
} From: Paula Allan-Wojtas
} Sent: Wednesday, June 20, 2001 7:47 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Printers and paper
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, all,
}
} This question has come up for us at a good time, with this ongoing
} discussion of printers.
}
} We recently purchased an Epson 880 ink jet printer for our EM/LM lab, and
} are very happy with it. The black and white prints look excellent. We have
} tried a number of papers, and find that the Epson "Photo Quality Glossy
} Paper" and "Photo Paper" give the best results for our purposes.
}
} We are wondering how to go about making our prints more permanent from
} these printers. If the prints have just been printed and aren't dry yet,
} or if they are dry and are in contact with any moisture, they smudge quite
} easily. Is there any coating - lamination or spray, or something else
} which we can use to protect the surface?
}
} In connection with this, we have also found, especially when we have
} printed b/w prints, that if we leave them in a room with full spectrum
} fluorescent lights for a few days, that they begin to change colour, and
} start to take on a reddish tinge. The instructions that came with the
} paper say to not leave prints exposed to sunlight - but we're talking
} about room lights, here! Has anyone else found this happens to them, and
} is there any way of preventing/minimizing this effect without storing
} prints in a light tight bag????
}
} Any help or suggestions would be much appreciated. As always, thanks for
} your help in advance.
}
} Regards,
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}
}
}


From daemon Thu Jun 21 13:25:42 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 21 Jun 2001 12:01:08 -0700
Subject: Re: Printing Gray scale on a deskjet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I checked out there system from a general interest, "this looks cool"
viewpoint. I recall the representative telling me that the technique works
by serial sections. I also seem to recall that the images are taken of the
remaining block rather than the removed sections.

It sounds like an interesting approach, and it will certainly have some
applications. I did not see any immediate applications in my research. I
was also unsure what kind of ultimate resolution could be achieved. It
seems that the technique would be as limited by the thickness of the
sections in the z-direction as it would by microscopy in the x- and
y-directions.

At 09:26 AM 6/21/2001 -0400, you wrote:
} Hi Sterling,
}
} We just had a demo of their system from the local sales person, and I must
} say I was greatly impressed. The company is very young and full of some
} great ideas. The sales crew seems eager to work with potential customers.
} The demo was good enough for the powers that be here to approve some "proof
} of concept" work with some of our own samples. The software is simply
} amazing in its potential. It seems to have a very user friendly, and
} intuitive interface that is quite powerful. Obviously the biggest
} limitation is resolution. Despite the claims of "high resolution", they
} are limited to typical light microscopy resolutions of about 0.2um/voxel.
} Another limitation is your material. If you are talking about biological
} samples, there is no problem, but currently certain materials do present a
} problem for them.
}
} If you have more specific questions about what I saw at the demo, please
} feel free to contact me.
}
} Sincerely,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}
}
} From: sterling stoudenmire



In my point of view, it's almost impossible to have gray tones with RGB (3
color scheme) color cartridges. CYMK (4 color) will do right if printer
set for gray-scale (to use black cartridge only). To simplify my life I
removed color cartridges and replaced them on black one for B&W
prints. For may model it's possible to use big black cartridge instead
color assembly. I always replace color ribbon on black one in our
Tektronix Dye-sub (actually, reverse: black ribbon installed by default and
we replace that on color if necessary).

Sergey

At 07:22 AM 6/21/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jun 21 14:18:26 2001



From: Tom Moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Thu, 21 Jun 2001 14:13:32 -0500
Subject: Re: Resolution Sciences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:53 PM 6/21/2001 -0500, Warren E Straszheim wrote:

I also looked into this a while back, it's an interesting approach.
Essentially the sample is fluorescently stained with something like eosin
and embedded in an opaque plastic. The surface is imaged, a micron or two
is shaved off, the new surface is imaged, and so on. This gives you a
(huge) 3D data set that can be viewed at various magnifications and
resolutions. You lease/buy a computer system from them, send them the
sample and they send you a DVD with the data. The software is quite fast
and easy to use. I haven't used their services yet, am still waiting for an
appropriate sample.

Tom


} I checked out there system from a general interest, "this looks cool"
} viewpoint. I recall the representative telling me that the technique works
} by serial sections. I also seem to recall that the images are taken of the
} remaining block rather than the removed sections.
}
} It sounds like an interesting approach, and it will certainly have some
} applications. I did not see any immediate applications in my research. I
} was also unsure what kind of ultimate resolution could be achieved. It
} seems that the technique would be as limited by the thickness of the
} sections in the z-direction as it would by microscopy in the x- and
} y-directions.

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.


From daemon Thu Jun 21 14:25:30 2001



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Thu, 21 Jun 2001 15:26:10 -0400
Subject: Re: biofilms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I guess my question would be "what is the investigator's definition of a
biofilm?" If you detergent-treat sediment, you are likely to remove a
substantial majority of the attached bacterial biomass from suspended
solids, but this will also contain non-biofilm (porewater) cells and the
majority of the spatial information in the sample (relationships of cells
to substrata, and of cells to cells) will be lost. Why do you want to
"isolate" the biofilms? Why not examine them directly (you are using
microscopy - virtually the ONLY way to examine biofilms directly).

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396


From daemon Thu Jun 21 14:34:04 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 21 Jun 2001 14:18:46 -0500
Subject: RE: Recommendations for BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used GW Electronics BSE systems for many years. I purchased my last
system just a few years back. With both the olde and new systems, I had
excellent results -performance is great. The equipment is very well made
and has been extremely reliable. I have no connection with GW other than
being an experineced and satisfied user.

GW can interface to just about any scope.

Woody White
McDermott Technology, Inc.
http://woody.white.home.att.net




} -----Original Message-----
} From: Johnson, Bradley R [mailto:Bradley.Johnson-at-pnl.gov]
} Sent: Thursday, June 21, 2001 11:54 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: SEM: Recommendations for BSE detectors
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hello All,
} I have a Jeol 5900 SEM, and I would like to purchase a
} BSE detector for
} it. Does anyone in the group have any
} suggestions/recommendations/warnings? Is
} it pretty straight forward to purchase a third-party detector
} and get it to work
} with our microscope, or is it best to go through the vendor?
} Thank you for any
} input that you can provide.
}
}
} Brad Johnson
} Pacific Northwest National Lab
} P.O. Box 99, K6-24
} Richland, WA 99352
} voice: 509-372-1635
} fax: 509-376-3108
}
} Bradley.Johnson-at-pnl.gov
}


From daemon Thu Jun 21 15:21:54 2001



From: Danowski, Kristine (KL) :      KLDanowski-at-dow.com
Date: Thu, 21 Jun 2001 15:16:31 -0500
Subject: duplicate posts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List,

Am I the only person receiving duplicate posts from the Microscopy list?

Just curious.

Kristine


From daemon Thu Jun 21 15:36:34 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 21 Jun 2001 16:31:25 -0400
Subject: Re: Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


craig klotz wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Would anyone who uses a MT-6000, Ultracut E, or NOVA ultramicrotome please
} comment on reliability, ease-of -use, lighting, etc. for those
} machines. Our lab is going to purchase a used machine in July.
}

We have had an Ultracut E for 15 years with only occasional and relativley
minor repairs needed. When we have needed service, Tek-Net of Lakewood, NJ has
been excellent.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Jun 21 16:28:16 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 21 Jun 2001 14:23:13 -0700
Subject: Fwd: Re: TEM-Ultrathin film grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Thu, 21 Jun 2001 14:22:08 -0700
} To: "Thearith H. Ung" {tung-at-qdots.com}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: TEM-Ultrathin film grid
}
} Dear Thearith
}
} 5 nm carbon film is very thick. I am using 1.5-1.8 nm carbon film
} on carbon 2 mkm holey film for 1.4 nm for NanoGold particles. You could
} also try dark-field mode to enhance the contrast. It's also possible to
} decrease the carbon film thickness because it's so strong. I measure the
} actual carbon thickness by TM-450 MAXTEK thickness monitor (0.01 nm
} resolution). I am using my own design "electron gun" and oil-free vacuum
} apparatus for carbon film preparation.
}
} Sergey
}
} At 09:51 AM 6/21/01 -0700, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jun 21 16:32:06 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 21 Jun 2001 14:25:48 -0700
Subject: Re: SEM: Recommendations for BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Bob Ruscica at ETP-USA. He handles Robinson
scintillator detectors. Probably the best type made.
Very sensitive, versatile and reliable. Not high cost, either.
They can be retrofitted to most any scope.

gary g. (highly satisfied Robinson BSE user)


At 08:54 AM 6/21/2001, you wrote:

} Hello All,
} I have a Jeol 5900 SEM, and I would like to purchase a BSE
} detector for
} it. Does anyone in the group have any
} suggestions/recommendations/warnings? Is
} it pretty straight forward to purchase a third-party detector and get it
} to work
} with our microscope, or is it best to go through the vendor? Thank you
} for any
} input that you can provide.
}
}
} Brad Johnson
} Pacific Northwest National Lab
} P.O. Box 99, K6-24
} Richland, WA 99352
} voice: 509-372-1635
} fax: 509-376-3108
}
} Bradley.Johnson-at-pnl.gov



From daemon Thu Jun 21 19:07:37 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 21 Jun 2001 17:00:03 -0700
Subject: Re: Printing Gray scale on a deskjet

Contents Retrieved from Microscopy Listserver Archives
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It should works great, thanks for idea. Basically, for my B&W job I am
using Tektronix dye-sub which performs fine.
Sergey

At 07:13 PM 6/21/01 -0400, you wrote:
} look for Quad-Black - it is a four or six grey shade ink set to replace
} the color ones - the prints have more grey levels than film.
}
}
} At 03:01 PM 6/21/01, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 21 22:29:02 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Thu, 21 Jun 2001 23:07:51 -0500
Subject: Microtome experiences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Craig,

I used an RMC MT-6000 XL for five years and I am using an Reichert
Ultracut E since a couple of weeks. After getting used to the Reichert I
like it a lot and think overall it is the better machine. It has some
nice features, for example this integral water pump and the specimen
trans-illumination etc. and I think the mechanics are more robust.
One thing is better about the RMC MT-6000 XL. You can change the angle
of the microscope and by that always get the best reflection of the
water surface in the knife boat (independent from how high the water
level is).
The RMC is really not bad but I think a lot of details make the Reichert
better.

Stefan







From daemon Thu Jun 21 22:30:33 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 21 Jun 2001 23:26:04 -0700
Subject: Re: Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
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Hi Craig,
As you probably know the machines you are asking about definitely have some
age on them. The lab here has a RMC MT-6000XL and an Ultracut E. I really
like the Ultracut - it works like a charm and has lighting in every
possible direction. I really dislike the design of the RMC MT-6000 and the
lighting is either on above or below but not both unless you get a switch
installed (we had the switch installed and it makes a big difference to
me). The newer RMC ultramicrotomes are very nice but the Ultracuts are
still the best ultramicrotomes. Can't comment on the Nova.
best regards,
Beth

} Would anyone who uses a MT-6000, Ultracut E, or NOVA ultramicrotome please
} comment on reliability, ease-of -use, lighting, etc. for those
} machines. Our lab is going to purchase a used machine in July.


**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************




From daemon Fri Jun 22 00:05:40 2001



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 20 Jun 2001 19:22:13 -0500
Subject: Re: Printers and paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For the more frugal try Johnson's Paste Wax. It works on some inks and
papers, not all. It does offer some protection against UV. It does protect
against moisture, finger prints, dust and scratches. It does change the
finish some.

If the wax causes the ink to smear a fixative spray will usually stop that.

Test it for several weeks before putting it on an expensive display.

Wax also works on regular photographs. The way I discovered it was on B&W
prints that were displayed outdoors in the weather for several months.

Gordon

Gordon Couger
Stillwater, OK



.From: "George Laing" {scisales-at-ngscorp.com}

Luminos http://www.lumijet.com has a spray called
"Imagesheild" to protect prints. $18/ can. Quoting their website:
"LUMIJET IMAGESHIELD will become your favorite print protector.
It's a convenient ozone-friendly low-odor aerosol spray that significantly
improves wet-fastness. In addition to protecting delicate inkjet images
from moisture, it also shields images from fingerprints and harmful UV rays.
This unique product will not only help extend useful print life,
it is totally transparent; preserving the original print surface
characteristics. From dead matte to glossy, or from canvas to silver foil,
you'll never know it's there."

} We are wondering how to go about making our prints more permanent
} from these printers. If the prints have just been printed and
} aren't dry yet, or if they are dry and are in contact with any
} moisture, they smudge quite easily. Is there any coating -
} lamination or spray, or something else which we can use to
} protect the surface?

There were recently (last 6-8 months) some issues with color shift
on some Epson papers. Epson determined the shift was from high
concentrations of ozone, and not due to exposure to light. Epson
recommends Heavyweight Matte or Epson Photo paper for maximum
lightfastness.


George

George Laing
National Graphic Supply
v:(800) 223-7130 X3109
f:(800) 832-2205
email: scisales-at-ngscorp.com
}
} In connection with this, we have also found, especially when we
} have printed b/w prints, that if we leave them in a room with
} full spectrum fluorescent lights for a few days, that they begin
} to change colour, and start to take on a reddish tinge. The
} instructions that came with the paper say to not leave prints
} exposed to sunlight - but we're talking about room lights, here!
} Has anyone else found this happens to them, and is there any way
} of preventing/minimizing this effect without storing prints in a
} light tight bag????
}





From daemon Fri Jun 22 04:07:15 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 22 Jun 2001 10:08:07 +0100
Subject: Re: Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Craig

I have used a 'Super Nova' for 11 or 12 years and the only thing that
has needed to be replaced/repaired was the fluorescent light source. We
are starting to have a niggling problem when switching on the light - it
can take several attempts and it looks like there might be some sort of
dedicated controller to be fixed. The only other thing that may need to
be replaced is the belt (motor drive band) which is beginning to slip
when manual raise and lower is used on the specimen arm. It's never
needed a service engineer though.
The reason we/I opted for the LKB was that I had used them for years and
liked their reliability - there is no thin section mechanical feed to go
wrong. The 'Super Nova' was a later model than the Nova and has a
completely sealed control panel which keeps the dust and probably water
out. It can also maintain specimen position accurately when you turn the
thermal feed off and the cut stroke is powered rather than just relying
on gravity. I don't know if these are standard on the Nova.
The Ultracut E has nicer optics, specimen adjustment is superb and it is
much less sensitive to external vibration but I cannot swear to it's
long term use because I've only used it in demonstrations.
I really think it would be worthwhile if you can get a demonstration
(especially if you can take a specimen to cut) because these machines
are all very good and it may come down to personal taste. One thing you
might to want to examine is the availability of spares, accessories and
servicing for each machine - in the UK it is becoming difficult to get
spares for the 'Super Nova'.

I hope this helps.

The usual disclaimers apply: my opinions etc. and no affiliations to any
company.


Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

craig klotz wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Would anyone who uses a MT-6000, Ultracut E, or NOVA ultramicrotome please
} comment on reliability, ease-of -use, lighting, etc. for those
} machines. Our lab is going to purchase a used machine in July.
}
} TIA,
} Craig


From daemon Fri Jun 22 07:38:20 2001



From: Helena Loflin :      Helena.Loflin-at-alconlabs.com
Date: Fri, 22 Jun 2001 07:28:16 -0500
Subject: Position Available -- Pharmaceutical Industry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Position: Scientist--EM / Histology (Reference Code 2232)
} Company: Alcon / R&D / In Vivo Pharmacology Unit
} Location: Global Headquarters in Fort Worth, TX
} Position Type: Full Time
}
} About Alcon...
}
} Superb facilities. Terrific benefits. Great, long lasting working
} relationships. Employee turnover of less than 5% annually. Fifty-three
} (53) years of steady, continuous growth. The $2.55 billion global leader
} in the ophthalmic and vision care industry. It's no wonder Fortune has
} selected Alcon as "one of the 100 Best Companies to work for" for three
} consecutive years. Our 10,000 employees worldwide form a unique community
} of multi-national, multi-disciplinary, dedicated and successful men and
} women who have combined their talents and vision over our 50-year history
} of growth and success. The tenure (average 13 years) and caliber of our
} employees is a major competitive advantage.
}
} For much more information about our people, benefits, products and
} culture, please visit us at
} www.alconlabs.com.
}
} Position Responsibilities
}
} * Prepares, processes, examines and provides preliminary
} interpretation of animal tissue specimens using various techniques with
} light microscopy and/or EM as end-point.
} * May provide method development (EM, histology, etc) directed towards
} the discovery of new drug candidates.
}
} Qualifications
}
} * Bachelor of Science degree in a related discipline
} * At least five years of significant light and/or EM experience
} related exclusively to human and animal tissue (including tissue
} preparation)
} * Collaborative and problem solving skills are essential
} * Highly refined interpersonal and technical writing skills
} * Certification by or eligibility to be certified by the MSA a plus
} * Traditional histology tissue preparation knowledge a benefit
} * Digital imaging, immunohistochemistry, and experience with ocular
} tissues highly desirable
}
} Alcon is an Equal Opportunity Employer committed to quality through
} diversity. M/F/D/V. Pre-employment drug testing.
}
} If you are interested in submitting your resume for this position, please
} email it to helena.loflin-at-alconlabs.com. Please include Reference Code
} 2232.
}
}
}


From daemon Fri Jun 22 08:16:36 2001



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Fri, 22 Jun 2001 15:09:47 +0200
Subject: JEOL 733: beam non-cooperation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
We had a short in the objective lens coil a while ago and it was
changed. What was happening was that we lost all control of the beam
position and focus. We usually work at 15 kV (suitable for most of our
needs), but now need to do something at 25. However, when we increase to
this acc. voltage, we are finding a similar problem - beam pops out of
focus (goes big and diffuse) and shoots off somewhere. We can get it
back by pressing the Obj. Pol. button. This normally works if we get the
same thing at 20 kV and less, but not at 25. The effect only occurs once
an hour at 22 kV. Anyone have an idea why this is happening? By the way,
for those who would undoubtably ask, the I is 100 nA for what we are at
the the moment.
Comments?
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA


From daemon Fri Jun 22 11:08:57 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 22 Jun 2001 09:01:38 -0700
Subject: Re: SEM: Recommendations for BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bradley,
I have used the JEOL BSE detector, the GW BSE detector and the Robinson BSE
detector and they all work well and I have had no reliability problems or
interface problems. For the third-party detectors, the external signal just
goes in through the "EXTERNAL" signal input on the SEM.
At 08:54 AM 06/21/2001 -0700, you wrote:
}
} Hello All,
} I have a Jeol 5900 SEM, and I would like to purchase a BSE detector for
} it. Does anyone in the group have any
suggestions/recommendations/warnings? Is
} it pretty straight forward to purchase a third-party detector and get it to
work
} with our microscope, or is it best to go through the vendor? Thank you for any
} input that you can provide.
}
}
} Brad Johnson
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Jun 22 11:36:27 2001



From: Guangwen Zhou :      guzst1+-at-pitt.edu
Date: Fri, 22 Jun 2001 12:38:45 -0400 (EDT)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim is right in what he says about the compatibility issues with Oxford/Link
detectors and the restore signal being generated by the pulse processor and
not the pre-amp as in most detectors. However we are reps for Thomson
Scientific Instruments' WINEDS x-ray analyzers and pulse processing
electronics in North America. TSI has the DX3000 bias and PX9000 Pulse
processor that will interface to the Oxford/Link detectors. We have used it
with both the older type and with the Pentafet type without any changes to
the detector preamp. The PX9000 is a universal design and can be used with
other manufacturers analyzers.

Douglas Connors
General Manager
TNAS Inc.
7897 Hwy 19, Dane WI 53529
(608)798-2005 voice
(608)798-1675 fax
tnas1-at-msn.com
----- Original Message -----
} From: "Jim Nicolino" {JNicolino-at-xraydetectors.com}
To: "Michael L. Boucher" {mboucher-at-tc.umn.edu}
Cc: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, June 19, 2001 10:30 AM




Please remove me from your mailing list

Thanks



From daemon Fri Jun 22 12:01:41 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Fri, 22 Jun 2001 09:57:12 -0700
Subject: Re: TEM-Ultrathin film grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you are only imaging the particles, not in context of a particular environment, you can negative stain e.g. with phosphotungstic acid.

Sounds like you're not considering staining them but if the particles are reactive then that is a possibility too.

Richard

} } } "Thearith H. Ung" {tung-at-qdots.com} 6/21/01 9:51:50 AM } } }

Dear colleagues,

I work with ultrasmall nanosized particles, as small as 2 nm, with much
lower contrast than metal particles such as gold and silver. These
ultrasmall particles don't show up very well on TEM, even with optimal TEM
settings. I thought what would help to increase the contrast dramatically
would be to use a TEM grid that has very low background signal. I have tried
grids with ultrathin carbon film (about 5 nm thick) but the background
signal is still too high for these particles to show up clearly. Holey
carbon grids is an option except they don't give me enough particles for
particle sizing. Any suggestions would be very much appreciated.


Regards,

Thearith





From daemon Fri Jun 22 13:39:37 2001



From: Rachel Spicer :      spicer-at-oeb.harvard.edu
Date: Fri, 22 Jun 2001 14:38:15 -0400
Subject: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
buffered fixative. I need to store the samples for a year or more - are
either of these fixatives appropriate for long-term storage, and if not,
what are the problems (e.g., tissue hardening?). Does anyone have
suggestions for appropriate storage solutions, or have an idea of how long
is OK in either of these fixatives? The samples in paraformaldehyde are
used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
Thanks in advance for your experience/advice.

Rachel

******************************************
Rachel Spicer
Biological Laboratories 3119
Organismic and Evolutionary Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

(617) 496-3580 (phone)
(617) 496-5854 (fax)
spicer-at-oeb.harvard.edu
******************************************



From daemon Fri Jun 22 15:17:43 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Fri, 22 Jun 2001 17:21:55 -0400
Subject: Re: TEM-Ultrathin film grid

Contents Retrieved from Microscopy Listserver Archives
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Hi Richard,

Thanks for the suggestion. I thought about staining as well, except that
these particles tend to get etched by acids. However, it might still work if
only a small amount of phosphotunstic acid is used. So it is definitely
worthwhile looking into it.

Thearith

-----Original Message-----
} From: Richard Thrift [mailto:Richard_Thrift-at-skyepharma.com]
Sent: Friday, June 22, 2001 9:57 AM
To: tung-at-qdots.com; Microscopy-at-sparc5.microscopy.com


Thearith,

Have you got a possibility to do STEM ? If there are some high Z atoms in your samples, you should
get enough contrast to see the particles.

Andreas

} Dear colleagues,
}
} I work with ultrasmall nanosized particles, as small as 2 nm, with much
} lower contrast than metal particles such as gold and silver. These
} ultrasmall particles don't show up very well on TEM, even with optimal TEM
} settings. I thought what would help to increase the contrast dramatically
} would be to use a TEM grid that has very low background signal. I have tried
} grids with ultrathin carbon film (about 5 nm thick) but the background
} signal is still too high for these particles to show up clearly. Holey
} carbon grids is an option except they don't give me enough particles for
} particle sizing. Any suggestions would be very much appreciated.
}
}
} Regards,
}
} Thearith
}
}
}
}



From daemon Fri Jun 22 17:12:40 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 22 Jun 2001 20:16:17 -0400 (EDT)
Subject: Re: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sounds like a contact problem with the OL polarity switch or relay.
At higher KV's the lenses need higher current. 22 KV is probably the limit
for the contact to cut-out.

Regards,

Earl



----- Original Message -----
} From: "Dr Malcolm Roberts" {m.roberts-at-ru.ac.za}
To: "Microscopy discussion group" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, June 22, 2001 6:09 AM


Hi Rachel...

I would imagine that storage in 70% ethanol might be a better bet...
brought up through an alcohol series. This is a standard practice for
long term storage of museum specimens after fixation, and for most EM
labs.

Check out following for reference: Simmons, J.E. 1995. Storage in Fluid
Perservatives. In Storage of Natural History Collections: A Preventative
Conservation Approach. Vol. 1. C.L. Rose, C.A. Hawks, and H.H. Genoways,
eds. Society for the Preservation of Natural History Collections, Iowa
City. 161-181.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Fri, 22 Jun 2001, Rachel Spicer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}



From daemon Sat Jun 23 01:52:35 2001



From: Aarti Harle :      aarti_harle_em-at-usa.net
Date: 23 Jun 2001 00:45:42 MDT
Subject: Re: [Re: Q re: long term storage in fixative]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Richel

If you want to store for long term then better preserve in a deep freezer at
-20 to -30 deg as it is
and when you want to proceed, allow it to come down at 4 deg and proceed as
per normal procedure.

Second option is fix in a fixative and then store in washing buffer at 4 deg
celcius, but keep one thing is in mind that you should keep changing this
buffer after a short time span, say 15-20 day. Your tissue well perserve in
this condition. Like this i have preserved the animal tissues in best of
condition for three months.

If you try to preserve in fixattive then their is a chacnes of hardening the
material or if you preserves in alchohol 70%, the chances of shrinkage is
more.

for best prservation methods Ref. the book by M.A. Hayat, Techniques in
Electron Microscopy.

With Best Regards
Arti Harle


Angela Klaus {avklaus-at-amnh.org} wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Rachel...

I would imagine that storage in 70% ethanol might be a better bet...
brought up through an alcohol series. This is a standard practice for
long term storage of museum specimens after fixation, and for most EM
labs.

Check out following for reference: Simmons, J.E. 1995. Storage in Fluid
Perservatives. In Storage of Natural History Collections: A Preventative
Conservation Approach. Vol. 1. C.L. Rose, C.A. Hawks, and H.H. Genoways,
eds. Society for the Preservation of Natural History Collections, Iowa
City. 161-181.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Fri, 22 Jun 2001, Rachel Spicer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}




Ms. Arti Harle
Regional Sophisticated Instrumentation Center
Indian Institute of Technology-Bombay (IIT-B)
Powai, Mumbai - 400076.
India
Phone : 91-22-5767691 Extn 7694
91-22-5720109/ 5721039 (Resi)
E-mail : aartih-at-usa.net


From daemon Sat Jun 23 08:45:30 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 23 Jun 2001 09:36:07 -0500
Subject: TEM nanoparticle contrast

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {200106231336.JAA08101-at-generic2.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-sparc5.microscopy.com}


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --


Thearith H. Ung wrote the following:
=========================================
I work with ultrasmall nanosized particles, as small as 2 nm, with much
lower contrast than metal particles such as gold and silver. These
ultrasmall particles don't show up very well on TEM, even with optimal TEM
settings. I thought what would help to increase the contrast dramatically
would be to use a TEM grid that has very low background signal. I have tried
grids with ultrathin carbon film (about 5 nm thick) but the background
signal is still too high for these particles to show up clearly. Holey
carbon grids is an option except they don't give me enough particles for
particle sizing. Any suggestions would be very much appreciated.
===============================================
This has become quite a generic problem for many who are working with nano
size particles, and many have found the "solution" to be the use of silicon
nitride membrane window grids that have been offered by SPI Supplies for
several years. Information about these grids can be found on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html

The silicon nitride is completely structureless and featureless and seems to
provide excellent electron transparency. The other advantage is that they
are sufficiently robust to survive temperatures in excess of 1000° C. And
there is no carbon grain to be confused with the nanoparticles of interest.

Disclaimer: SPI Supplies offers silicon nitride membrane window grids for
this type of application.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Jun 23 08:45:30 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 23 Jun 2001 23:35:21 +1000
Subject: RE: TEM-Ultrathin film grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are other negative staining compounds available - ammonium molybdate and
uranyl acetate for instance.
However, my first try would be shadow-casting, by evaporating some Pt/C at
about 10 degrees to the specimen.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, June 23, 2001 6:06 AM, Thearith H. Ung [SMTP:tung-at-qdots.com]
wrote:
}
}
}
} Hi Richard,
}
} Thanks for the suggestion. I thought about staining as well, except that
} these particles tend to get etched by acids. However, it might still work if
} only a small amount of phosphotunstic acid is used. So it is definitely
} worthwhile looking into it.
}
} Thearith
}
} -----Original Message-----
} } From: Richard Thrift [mailto:Richard_Thrift-at-skyepharma.com]
} Sent: Friday, June 22, 2001 9:57 AM
} To: tung-at-qdots.com; Microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM-Ultrathin film grid
}
}
} If you are only imaging the particles, not in context of a particular
} environment, you can negative stain e.g. with phosphotungstic acid.
}
} Sounds like you're not considering staining them but if the particles are
} reactive then that is a possibility too.
}
} Richard


From daemon Sat Jun 23 11:57:27 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Sat, 23 Jun 2001 12:33:25 -0500
Subject: Just For Fun Micrograph Contest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Readers,
Kindly allow this short reminder that again at the M&M 2001 Conference we
will hold a "Just For Fun Micrograph" Contest. The concept being composite
images, each of two or more images, one of which must be microscopical in
nature. $600 cash in prizes.
Contact me direct and I will provide full detail.
Don Grimes, Microscopy Today



From daemon Sun Jun 24 15:54:24 2001



From: mario:      jane0l2l-at-excite.com
Date: Wed, 20 Jun 2001 02:27:01
Subject: Manuf. Production/Contrl Software For $1,495.00.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Job Master, a complete, user friendly Windows based software package, can manage and control your operation from sales quote to shipment.

For one week only, Job Master, normally $2,495.00, is on sale for a total price of $1,495.00. In order for you to receive this $1,000.00 savings we must have your order by June 22th.

Job Master is designed specifically for small to medium sized manufacturers, and costs many thousands of dollars less than any other even remotely comparable software package.

Following is a list of features. If you have any questions, would like to discuss the package further, or if you would like to obtain our Web site address for a total walk through of the program, please call me directly at (661)254-9926(please do not E Mail me back. I may not get your message if you simply hit "Reply" and respond to this message via return E Mail).

By way of background, we are a software company, which for some years has specialized in the development of custom software, primarily for small to medium sized manufacturers. Job Master is a distillation of over a million and a half dollars of software we have developed to control and manage the production of our manufacturing clients.

Job Master contains the following features:

1. QUOTATION MODULE. In this module, quotes are developed, modified, and produced for sending to your client. A history is kept of all quotes for future reference, or modification for other clients. All quotations and revisions are "auto numbered," including versions. The quotes section allows for the entry of parts/processes, and costing of each, including materials, labor, markup, and taxes. Inventory status can be accessed from this section for reference.

2. SALES ORDER. Once a quotation is accepted, the final quotation information can be transformed into a Sales Order for your client's signature on a "point and click" basis. The Sales Order can be modified and re issued if necessary. A history if kept of all Sales Orders for future reference, or modification for other clients. All sales orders and revisions are "auto numbered," including versions. Inventory status can be accessed from this section for reference.

3. CUSTOMER LETTERS can be created from the Quotation and Sales Order sections.

4. SHOP TRAVELER/WORK ORDER. Once a Sales Order is accepted, the sales order information can be transformed into a shop traveler/work order on a "point and click" basis. Each item on the Sales Order becomes a shop traveler/work order, with each step of production of the item then listed on the traveler/work order. Each such traveler/work order is tied back into the Sales Order. The shop traveler/work order allows for the entry of line items, and notes on each line item. The shop traveler/work order contains a "notes" section. The Shop traveler/work order allows for the storing or attachment of drawings to the traveler/work order. The shop traveler/work order also contains a "drop down," from which standard processes can be selected for inclusion on the shop traveler/work order. The shop traveler/work order numbers progress in order of production sequence, and re numbers them if new steps are added. The shop traveler/work order allows for change orders or revisions, and numbers changes in sequence of
he original shop traveler/work order number; i.e., 100, 100-1, 100-2, etc. All shop traveler/work orders and related revisions are retained in memory for future reference. The shop traveler/work order is bar coded for tracking of production step by step, and production of ongoing client status reports. Bar coding includes the ability for an employee to "swipe" their own ID bar code for recording in the system as to who upgraded what step. The shop traveler/work order function also allows for manual update of production status. The shop traveler/work order allows for quality control sign off, and the final production of certifications, either from a "canned" list, or hand typed in on a case by case basis.

5. INVENTORY. The application includes an inventory section, which allows operations to check materials inventory in and out. The inventory section allows for the comparison of inventory received against a P.O., and produces an "overage/underage" report of inventory received as compared against the P.O. The inventory section allows for the setting of minimum (re-order now!) and maximum inventory amounts, and produces reports showing what inventory needs to be ordered, as well as inventory that is at or above the maximum set to have in house. The inventory section also tracks "partially shipped" orders, which are tied in to the shipping function. This section shows how much completed product under a particular order has been actually shipped to a client, and how much remains to be shipped. The balance is adjusted as shipments are made.

6. REQUEST FOR PURCHASE. The application allows operators to produce a Request For Purchase for accounting for any inventory items, which need to be ordered. Inventory items have a drop down of approved vendors for each item.

7. REQUEST FOR BID. The application allows operators to produce a Request For Bid for accounting to send to Vendors for any inventory items, which need to be ordered. Inventory items have a drop down of approved vendors for each item to which Requests For Bid can be sent.

8. INVOICE. The application produces an invoice/invoice detail for all completed items ready to be billed/shipped to clients.

9. PRODUCTION OUTPUT STATUS. The application produces a date range selectable report on how much product, and the value of the product, which was completed during a selected date range. The application also produces a report on how many orders, and the value of those orders, which remain to be completed during a selected date range.

10. The application produces SHIPPING DOCUMENTS as per selected shippers, and produces a PACKING SLIP.

11. The application has a "FIND" FUNCTION in selected sections, allowing for searches by customer name, work order number, etc.

12. The application has "AUTO FILL;" i.e., when an operator starts to type in a name, number, etc. all related information auto fills after the first few letters or numbers are typed in.

Job Master is currently being sold in the marketplace for $2,495.00 per package. However, if we receive your order by June 22th, your total price will be $1,495.00.

Again, if you have any questions at all, or would like to place your order, please call me on my direct line, (661) 254-9926. Thank you!


Mario Chavez
Application Sales, Inc.

----------------------------------------------------------------------

You have received this newsletter because you signed up for updates on our tracking software. If you want to unsubscribe from this newsletter, please send a reply email with "REMOVE" in the subject line.


From daemon Sun Jun 24 22:03:52 2001



From: Beverly Giammara :      giammara-at-bellsouth.net
Date: Sun, 24 Jun 2001 23:13:22 -0400
Subject: EM Service vs Manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This has been discussed before, but... If anyone has a summary regarding
contract services that they could share, I would appreciate it. (They
seem to be lower priced than electon microscope vendors.) Any recent
experience positive or negative with contract services, particularly 1)
Advanced Research Systems (Alan Sampson) in Illinois or 2) Sci. Inst. &
Applications (V. Feingold) of Duluth, GA or 3) Other EM service
providers covering the Midwest. Thanks for your help.



From daemon Sun Jun 24 23:31:07 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Mon, 25 Jun 2001 14:28:00 +1000
Subject: Re: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi Rachel,

Remember that paraformaldehyde is a reversible fixative, so it isn't
appropriate to fix in pform then store in a buffer. I've stored
animal tissue for years in pform with no problems, though I'm sure
it's not recommended. As for pform/glut, storage after fixation in
cacodylate buffer is good, as nothing seems to grow in cacodylate.

Diana

}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Mon Jun 25 03:32:22 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 25 Jun 2001 09:25:44 +0100 (GMT Daylight Time)
Subject: Re: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No relevant experience but ...

How about storing part of the sample in buffer with sodium
azide to inhibit microorganisms.

Dave


On Fri, 22 Jun 2001 14:38:15 -0400 Rachel Spicer
{spicer-at-oeb.harvard.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Jun 25 07:44:32 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 25 Jun 2001 13:36:57 +0100 (GMT Daylight Time)
Subject: Re: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have found that there are a few cacodylate tolerant
organisms. Nonetheless I usually take the risk.

Dave


On Mon, 25 Jun 2001 14:28:00 +1000 Diana van Driel
{dianavd-at-eye.usyd.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Hi Rachel,
}
} Remember that paraformaldehyde is a reversible fixative, so it isn't
} appropriate to fix in pform then store in a buffer. I've stored
} animal tissue for years in pform with no problems, though I'm sure
} it's not recommended. As for pform/glut, storage after fixation in
} cacodylate buffer is good, as nothing seems to grow in cacodylate.
}
} Diana
}
} }
} } Hello,
} }
} } I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} } and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} } buffered fixative. I need to store the samples for a year or more - are
} } either of these fixatives appropriate for long-term storage, and if not,
} } what are the problems (e.g., tissue hardening?). Does anyone have
} } suggestions for appropriate storage solutions, or have an idea of how long
} } is OK in either of these fixatives? The samples in paraformaldehyde are
} } used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} } Thanks in advance for your experience/advice.
} }
} } Rachel
} --
}
}
}
} Diana van Driel
} Department of Clinical Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney NSW
} AUSTRALIA 2001
}
} Phone 61 2 938 27278/27395
} Mob 0412 165 075
} Fax 61 2 938 27318
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Jun 25 10:29:31 2001



From: Jill Verlander Reed :      verlaj-at-medicine.ufl.edu
Date: Mon, 25 Jun 2001 11:19:49 -0500
Subject: EM Service vs Manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists -

Beverly Giammara wrote asking for comments about EM service providers,
other than the manufacturers, that cover the Midwest. I would be very
appreciative if anyone can offer similar information about EM Service
providers that cover Florida.

Sincerely,

Jill

Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299


From daemon Mon Jun 25 11:03:29 2001



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Mon, 25 Jun 2001 11:58:21 -0400
Subject: TEM / X-ray: Simulation of diffraction patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are currently assessing whether or not to replace our old diffraction
simulation software and would in this respect be happy to receive the advice
of the microscopy community and vendors.

The software should have the following characteristics:
* Electron diffraction simulation, kinematical as well as dynamical
calculation.
* Handle multiple reciprocal lattices in one pattern - that is - several
single crystals with different crystallography, size, shape, and orientation
relationships, as well as powder diffraction.
* Accurate intensity and structure factor determination.
* Evaluate double diffraction and crystal shape effects - streaking.
* The software should also be able to simulate X-ray diffraction patterns:
powder, Laue, Guinier.
* Support of common crystallographic/diffraction database formats.
* The software should run under Windows NT 4.5 and have a professionally
designed GUI. No compiling and linking, and no programming should be
necessary but the possibility of doing this (e.g. scripting, ActiveX,
Plug-Ins etc.) is a very positive feature.

Sincerely,
Paul Baggethun
==========================
Paul Baggethun
Alcoa Technical Center
Alcoa Center, PA 15069
USA
(724) 337 1760
paul.baggethun-at-alcoa.com
==========================


From daemon Mon Jun 25 12:34:48 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 25 Jun 2001 13:27:30 -0400
Subject: RE: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rachel,

Please forgive. My first rule of subjugation is: "They can only
learn and do what they are taught!" Thus, one hopes that you will NOT take
umbrage with what follows. However, your teachers, ......

As you are in such a "Divine" location, I am hesitant to suggest
anything, especially in light of your use of such holy words of biology as
"chunks" and "paraformaldehyde". I will, however, and only with great
temerity and the deepest humility, suggest that you try to gain access to a
tome of non-Harvardian origin entitled, "Principles and Techniques of
Electron Microscopy"(3d ed.), Hayat, M.A.[he once worked in Newark, NJ!!!],
CRC Press, Boca Raton, FL(1989), pp. 69-70 (sections entitled:
"Simultaneous Fixation for Light and Electron Microscopy", and "Tissue
Storage".) ISBN: 0-8493-7111-2. My humility is manifested for the
divinity, but my temerity is evoked by the ignorance. However, Hayat begins
one paragraph with the following sentence, "The size of the tissue specimen
and the objective of the study primarily determine the type of the fixative
solution to be used." He further mentions 4% formaldehyde and 1%
glutaraldehyde in a buffer at pH 7.4 with an osmolality of ~200mo for
"medium" pieces (~3mm) for longer times up to 12 months. Plants get
specific treatment on pp. 72-73. There are other books, though I must
admit, notwithstanding the efforts of Science Librarians, most of them have
been 'lifted' by the new wave of dedicated students and others during the
past 30 years. Try the 'older' members of the biology faculty -
non-molecular, if possible, - and remember that HCHO has been used in some
states to INDUCE fluorescence in tissues!

Again, PLEASE forgive. The last time I spoke to a student, a
pre-med by-the-by, I didn't understand him either.

We old-timers are severely challenged, because we were trained to:

choose a vocational path to follow,
ask questions after we had gone to the library,
judge Timothy Leary as the short form of a donkey,
understand women as a great mystery of the universe,
leave the gloves off when dissecting,
PLEASE understand, and NOT ask about the war,
use slide rules (to do logs), planimiters (to do areas), and
Leroy Lettering Sets (to label figures),
understand the divine nature of (inspiration!) discovery
take very deep breaths in the lab during days of dissection,

mix formaldehyde ("Formalin") and phenol to make good
embalming fluid,
squirt phenol on a sponge and wipe to disinfect lab benches
in bacteriology,
know that et cetera WAS Latin not Siamese(now Thai),
take deep breaths in bacteriology lab,
accumulate good books while they were relatively
inexpensive,
know that phenol was hygroscopic,
cut sections from any tissue as long as it came from an
unlabelled jar,
label the jar,
remember Latin and Greek roots (On Divinity Avenue, is that
"dis-" as in "to separate" or dice-" as in "to chop up"?),
do our income taxes on cards,
tell students off,
treat your fiancée' as the virgin you hoped she was,
know the difference between formaldehyde and
paraformaldehyde,
keep the lab windows open during dissections to keep your
eyes from burning,
cultivate your wife as your best friend,
ignore HIV and gHSV - no one had ever heard of them,
be terrified of pregnancy before marriage (the greatest
deterrent of STD's in the 20th century!), and
know the difference between discovery and invention (a la,
polymerase, la la, and the PCR, la la).

We were taught BY the organic chemists to pay very strict attention
TO the organic chemists, because they were said to know most of the
important secrets. The physical chemists, at the time, were busily
unraveling the secrets of life - only one 'A' every five years among
hundreds of chemists! Biochemists were chemists. Biologists cultured
cells, developed vaccines, cut sections, referenced papers from the 1860's,
didn't teach DNA until the early 1960's when protein genes were defeated at
the polls, and, just now, may be getting - in another decade - to the point
where the calculus may have a place in their courses.
In the 1950's, or so went the legend, Harvard Medical School
(Heaven's Gate?) sent representatives to Lehigh, where my teachers worked,
to discover the secrets of chemical pedagogy that prepared LU graduates so
well for the divine medical training some few received at the hands of
Harvard's Professeurs! The secret pedagogical trick they discovered at
Lehigh consisted of: "NO MERCY!", "NO SYMPATHY!" and "TOTAL RIGOR!", the
only means ever devised to overcome the (utter) shame of being considered
near to, but nonetheless, NOT, the equal of those "IVY" places.

If you didn't have fun, I wish you had. AND, before you store in
buffer, think about it (extraction of fixative and other things) long and
hard!

Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Rachel Spicer
} Sent: Friday, June 22, 2001 2:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Q re: long term storage in fixative
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}


From daemon Mon Jun 25 13:25:17 2001



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Mon, 25 Jun 2001 13:18:45 -0500
Subject: Re: AFM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Arti,

Novascan Technologies has a great deal of experience in the area of
biochemical AFM and I'm sure we can help you with your project We have
been developing AFM products for several years, but our specialty is
fabrication of AFM probes and surfaces with specific surface chemistries.
These chemistries, for example, can be used to couple molecules to surfaces
for imaging and/or molecular characterization. Please contact us at
info-at-novascan.com or visit www.novascan.com

Best Wishes,

Raj


At 04:17 AM 06/20/2001 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies, Inc.
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
**********************************************
"Innovative Tools to Explore the Microworld"


From daemon Mon Jun 25 13:38:53 2001



From: Vachik Hacopian :      vhacopian-at-wellesley.edu
Date: Mon, 25 Jun 2001 14:32:42 -0400
Subject: critical point dryer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In our lab in Massachusetts, we have a Balzers CPD-020 that is in need of
repair. The pressure guage (WIKA, 0-160 bar) is leaking, and we'd like to
have it replaced. This unit was made in Switzerland, but the manufacturer
has been out-of-business for many years. We'd appreciate input from
Balzers CPD owners regarding sources for parts. Any comments concerning
safety issues would be welcomed also. Many thanks in advance.

Vachik Hacopian





From daemon Mon Jun 25 14:16:43 2001



From: Julian Martinez-Fernandez :      Julian.Martinez-Fernandez-at-grc.nasa.gov
Date: Mon, 25 Jun 2001 15:11:13 -0400
Subject: High Resolution Imaging Plate Scanner for Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody have experience with High Resolution Imaging Plate Scanner for
Electron Microscopy (http://www.ditabis.de). Instead of conventional
EM-films, flexible photostimulable phosphor screens of the same size, the
so-called Imaging Plates, are loaded into the TEM magazine. Are they as
good as negatives?

Thanks,
Julian

_______________________________
Julián Martínez Fernández
Senior Research Associate
MS 106-5 Ceramic Branch
NASA Glenn Research Center
Cleveland, OH 44135
Phone: 1-216-433-5512
Fax: 1-216-433-5544



From daemon Mon Jun 25 14:56:28 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 26 Jun 2001 07:55:16 GMT+1200
Subject: Re: SEM: Recommendations for BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You may find that only the JEOL one will properly fit in, I
investigated a GW Electronics one for my JEOL 840, but they didn't
have a size that would fit exactly. Mind you, I have a (JEOL)
optical microscope fitted, and the BSE detector would have had to
fit into a severely constrained space on the end of the OM.

I would ask JEOL first, I find that their prices are sometimes better
than those from third-party suppliers anyway.


}
}
} Hello All,
} I have a Jeol 5900 SEM, and I would like to purchase a BSE detector
} for
} it. Does anyone in the group have any
} suggestions/recommendations/warnings? Is it pretty straight forward
} to purchase a third-party detector and get it to work with our
} microscope, or is it best to go through the vendor? Thank you for
} any input that you can provide.
}

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Jun 25 17:25:25 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 25 Jun 2001 15:11:25 -0700 (PDT)
Subject: Electroscan ESEM model E3 questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We just inherited an older model (10 years old) model E-3 Electroscan
Environmental SEM. I have some questions in its operating vacuum modes as
it has been quite a bit picky since we've installed it.

On going from a standby vacuum state into either Hivac or Wet vacuum
modes, it will often have an error of can't pump column EC2. Also, in Wet
mode, it will not open valve V1, so you can never see the beam. I've read
over the manuals, but I was wondering if anyone out there has some
experience with this model ESEM. We are thinking that one of the
diffusion pump heater elements needs to be changed. Other times we
operate the unit, it will maintain a Hivac vacuum mode for several hours
without difficulty.

Any advice or comments, appreciated, Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Mon Jun 25 22:31:23 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 25 Jun 2001 20:20:36 -0700
Subject: Re: High Resolution Imaging Plate Scanner for Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did study this subject a few years ago and find that this technique is
not well optimized for EM. For many reasons:

- cost of the system is similar to the EM digital camera
- you require to load/unload Imaging Plates into the cassettes (you waste
the time)
- it takes from 2 to 5 min to scan one Image Plate (waste time)
- I am not sure exactly, but seems to me, that you have to scan Image
Plates soon after tacking picture, otherwise the image will deteriorated,
and seems to me they are sensitive to light too.
- you don't have a chance to adjust your image before recording (as it
happens in case of digital cameras).
-number of "shots" is limited and Image Plates are princely
- Image Plates may be damaged during loading/unloading which may reduce
declared number of use (a few thousand times I believe).

The good things about Image Plates are:
- Image plate has more pixels than EM digital cameras, so the resolution is
better.
- you don't have to make any changes in the microscope.
- the linearity and dynamic range is similar to the EM digital cameras
- easy to use and less problem related to the equipment.

My conclusion was: technically, it's a great stuff, but it does not help in
practice. You still need some help from technician to load/unload plates
and scan them and you have to pay a 20-40$K for that beauty. Not so
promising technology for TEM at least.

This information based on my "study" performed a few years ago and may not
reflect modern improvements in this area.

Sergey.



At 03:11 PM 6/25/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Jun 26 01:50:29 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 25 Jun 2001 23:42:25 -0700
Subject: Re: High Resolution Imaging Plate Scanner for Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was talking about particular DITABIS system Julián was mentioning in her
original message (with reference to the manufacturer).

All digital cameras including Gatans are approximately 10 times more
sensitive than 4489 film. I know it for sure because just returned back
from Gatan's demonstration. Image Plate should have very similar
characteristics in terms of sensitivity, dynamic range and linearity as
modern digital cameras. The best things about Image Plates it's their
pixel size: 5000 x 4500 total pixels per plate. It's greater than in
digital cameras: 1000x1000/2000 in most cases. It's still less than you
could get from film with $800 scanner: 6400x4800 for 1600 optical dpi.
BUT!!!
You have LOAD/UNLOAD plates into cassettes manually. In the dark. Stack
them and load into the scanner (so, you have to have space for the scanner
in dark-room). Scanner will process them automatically (and at that time
you could not use dark-room for regular things, light from scanner). Load
them back into cassettes, degas magazine and load it into microscope. I
don't remember exactly, but it seems to me the cost of one plate is
something around $100. So, I have two magazines with 50 cassettes
each: 100x100$ plus scanner plus salary for technician plus computer and
storage media. Ok, I forgot to mention that you have to ERASE plates
before use, also, using special eraser. I don't remember, how many plates
you could ERASE in one run. May be it's automatic too. Scanning: 3.5 min
per plate x 50= 3 h.

As I mentioned before, the technique is great, but not so helpful for
regular day-to-day basis EM and does not save time so much. It works fine
for molecular biology applications in "phospho-imagers" but people need to
scan a few plates per day (even one a week) and they save a lot of
time: 10-15 min exposure vs 5-10 days! I just don't see reason to switch
to this technique to myself. If I'll have such money, I would buy digital
camera. It's more practical solution from my point of view.

Sergey

At 01:36 AM 6/26/01 -0500, you wrote:
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Sergey,
}
} I last saw the unit demonstrated at the EUREM meeting in Brno last summer.
} It is not as you described it. I won't say it is better but overall it is
} comparable to the Gatan product.
}
} What impressed me the most about it is that
}
} a) exposure times are comparable to what is now used for conventional film
} (the Gatan unit has long exposure times) and
}
} b) while the scanning step (of the digital plates) takes some time, it does
} it all automatically, so there is no babysitting with the scanner. You are
} free to do other things including exposing more digital plates.
}
} They might have one at the MSA meeting in Long Beach.
}
} Chuck
} SPI Supplies
} -------- REPLY, Original message follows --------
}
} } Date: Monday, 25-Jun-01 08:20 PM
} }
} } From: Ryazantsev, Sergey, Ph. D. \ Internet: (sryazant-at-ucla.edu)
} } To: Julian Martinez-Fernandez \ Internet:
} } (julian.martinez-fernandez-at-grc.na)
} } To: MICROSCOPY BB \ Internet:
} } (microscopy-at-sparc5.microscopy.com)
} }
} } Subject: Re: High Resolution Imaging Plate Scanner for Electron
} Microscopy
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To
} } Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-
} Line
} } Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I did study this subject a few years ago and find that this technique is
} not
} } well optimized for EM. For many reasons:
} }
} } - cost of the system is similar to the EM digital camera
} } - you require to load/unload Imaging Plates into the cassettes (you waste
} } the time)
} } - it takes from 2 to 5 min to scan one Image Plate (waste time)
} } - I am not sure exactly, but seems to me, that you have to scan Image
} } Plates soon after tacking picture, otherwise the image will deteriorated,
} } and seems to me they are sensitive to light too.
} } - you don't have a chance to adjust your image before recording (as it
} } happens in case of digital cameras).
} } -number of "shots" is limited and Image Plates are princely
} } - Image Plates may be damaged during loading/unloading which may reduce
} } declared number of use (a few thousand times I believe).
} }
} } The good things about Image Plates are:
} } - Image plate has more pixels than EM digital cameras, so the resolution
} is
} } better.
} } - you don't have to make any changes in the microscope.
} } - the linearity and dynamic range is similar to the EM digital cameras
} } - easy to use and less problem related to the equipment.
} }
} } My conclusion was: technically, it's a great stuff, but it does not help
} in
} } practice. You still need some help from technician to load/unload plates
} and
} } scan them and you have to pay a 20-40$K for that beauty. Not so
} promising
} } technology for TEM at least.
} }
} } This information based on my "study" performed a few years ago and may not
}
} } reflect modern improvements in this area.
} }
} } Sergey.
} }
} }
} }
} } At 03:11 PM 6/25/01 -0400, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Does anybody have experience with High Resolution Imaging Plate Scanner
} for
} } } Electron Microscopy (http://www.ditabis.de). Instead of conventional
} } } EM-films, flexible photostimulable phosphor screens of the same size, the
}
} } } so-called Imaging Plates, are loaded into the TEM magazine. Are they as
} } } good as negatives?
} } }
} } } Thanks,
} } } Julian
} } }
} } } _______________________________
} } } Julián Martínez Fernández
} } } Senior Research Associate
} } } MS 106-5 Ceramic Branch
} } } NASA Glenn Research Center
} } } Cleveland, OH 44135
} } } Phone: 1-216-433-5512
} } } Fax: 1-216-433-5544
} } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
}
} -------- REPLY, End of original message --------

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Jun 26 06:36:45 2001



From: Steve Widing :      swiding-at-astro.ocis.temple.edu
Date: Tue, 26 Jun 2001 07:28:48 -0400 (EDT)
Subject: Re: High Resolution Imaging Plate Scanner for Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Julian,

I contacted ditabis several months ago. Beyond a confirmation email from
the company, I haven't received any from them.


Steve Widing
Temple University


On Mon, 25 Jun 2001, Julian Martinez-Fernandez wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anybody have experience with High Resolution Imaging Plate Scanner for
} Electron Microscopy (http://www.ditabis.de). Instead of conventional
} EM-films, flexible photostimulable phosphor screens of the same size, the
} so-called Imaging Plates, are loaded into the TEM magazine. Are they as
} good as negatives?
}
} Thanks,
} Julian
}
} _______________________________
} Julián Martínez Fernández
} Senior Research Associate
} MS 106-5 Ceramic Branch
} NASA Glenn Research Center
} Cleveland, OH 44135
} Phone: 1-216-433-5512
} Fax: 1-216-433-5544
}
}



From daemon Tue Jun 26 09:21:48 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 26 Jun 2001 09:54:21 -0400
Subject: EM Service vs Manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jill:

For electron microscope service try Vitaly Feingold who is based out of
Georgia. His references are excellent!! His phone number is 770-232-7785.
E-mail address is: vitalylazar-at-worldnet.att.net

-----Original Message-----
} From: Jill Verlander Reed [mailto:verlaj-at-medicine.ufl.edu]
Sent: Monday, June 25, 2001 12:20 PM
To: Microscopy-at-sparc5.microscopy.com


Fellow microscopists -

Beverly Giammara wrote asking for comments about EM service
providers,
other than the manufacturers, that cover the Midwest. I would be very
appreciative if anyone can offer similar information about EM Service
providers that cover Florida.

Sincerely,

Jill

Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299


From daemon Tue Jun 26 09:22:35 2001



From: ilovetomail-at-runbox.com
Date: Tue, 26 Jun 2001 15:17:53 +0100
Subject: UK Number Provider

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{a href="http://www.keylinecom.com"} KEYLINE!!! {A}



From daemon Tue Jun 26 09:30:44 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 26 Jun 2001 10:25:56 -0400
Subject: RE: Q re: long term storage in fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rachel,
After an entire evening of self-immolation, I have returned to the
table to extend my apology for taking advantage of you yesterday. I really
do apologize. I looked across the table at my own daughter last evening,
and I realized that she might just have failed to see any humor at all in my
response had she requested the same information. Having realized that
possibility, I spent the rest of the evening in shock at my probable
insensitivity.
As an explanation, you received, in response to your entirely
innocent query, a message composed of every quip I have wanted to make to my
children, their teachers, their teachers' parents, my students, and probably
a few I can't remember. I thought to make it humorous, and there are some
who have decided that I succeeded, but I would suspect that you may have
felt somewhat differently. Thus, this message in self-rebuttal. Not nearly
as long, and hopefully more to the point.
If you had walked into my office with the question you asked, I
would have said, "Miss Spicer, we do not use words like "chunks" to describe
specimens from which we wish to extract reasonable scientific information."
You would have responded with some other nebulous term for the objects with
which you were contesting, and I would have cajoled you, with a couple
suggestions on precision in thinking and describing, to go to the library
and start doing what you are obviously trying to learn how to do - namely,
research.
So, please let me begin again. My second rule is: "They only learn
to think if they are forced." In that regard, I would have requested that
you make a list of the steps in the process you are asked implement. For
each step in such a process, there must be a purpose. For each step, there
must be a prescribed outcome (I have come to despise that word!), and for
each step, there must be BACKGROUND/HISTORY. My third rule is this. "The
most difficult, and the most successful research for a bench scientist is
done in the brain, the office, and/or the library." The work at the bench
only certifies or debunks what was done before, but the same principal holds
for even the most minor part of the process.
You suggest that you must to store plant/wood tissue for a year.
Most of us would want to know why? Why must you wait to at least embed.
Why would you want to soak/extract the tissue for that length of time before
acting on the NEXT step in the procedure. It is apparently not that you
wish to archive the tissue/wood. So, with your list in your hand, the
question for that step is, "Why?" "What is the purpose/reason for waiting?"
I certainly cannot help you to progress in the trek to an answer
until I know. Why? I'll give you an example.
There was an undergraduate who accosted me in the hall during my
first week as a graduate student. He asked me to point him in the direction
of distilled water. I asked him what type he wanted. He assumed I was
harassing him and told me to knock off the B.S. and just, "point!" I did.
Unfortunately, his purpose was to subculture honey bee cells, and the
'distilled' water to which I pointed - the nearest - was made in a chromium
plated metal still. Notwithstanding his rudeness, I should have insisted -
through instruction, but I was fresh out of the military and I didn't have
any time left for stupidity and self-destructiveness, Fourteen weeks
later, he blamed me for the failure of his project - my first real interface
with a baby boomer of the 'gimmee' type. I only felt sympathy for the
instructor, but his advice (he had been in the military too) was to learn to
take responsibility for the errors of students, for, someday I would be
their teacher.

Over the past thirty years, what was a two-way street in learning
when I was a student, has become one-way.
I guess the ultimate point is this. If you are working on someone
else's project, show him or her my notes. If you are working on a project
of your own, and you are willing to exercise patience and learn, then when
you ask for information from us - the MSA ListServer - you must provide us
with specific information. I can promise you that you failed to receive
many responses, because your question lacked three elements:

1. Precise terminology.
2. Purpose/Goal/Reason for need.
3. Trust.

Everyone on the ListServer has a job or values his/her time.
Your request was informal in form, because it lacked 1) precise detail, 2)
a reason for wanting to delay further processing (that would be important
for any specific response), and 3) trust in us to be honest brokers and not
steal your project or question its value. Far too many visitors to the
Listserver ask a question as if in class, at the end of a lecture. We all
came in late.

I hope you want to try again, if what little you have
received has not helped. We would like to help as much as possible. We are
here for that. Not for entertainment. I am here, because when I read the
messages, I learn or have questions that I can address. I am here to be
educated, just like you.

With closing apology for wordiness and philosophy, I am

Yours sincerely,

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.





} ----------
} From: Rachel Spicer
} Sent: Friday, June 22, 2001 2:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Q re: long term storage in fixative
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I'm fixing small chunks of wood in 4% phosphate buffered paraformaldehyde,
} and separately in a Karnofsy-type (paraformaldehyde and glutaraldehyde)
} buffered fixative. I need to store the samples for a year or more - are
} either of these fixatives appropriate for long-term storage, and if not,
} what are the problems (e.g., tissue hardening?). Does anyone have
} suggestions for appropriate storage solutions, or have an idea of how long
} is OK in either of these fixatives? The samples in paraformaldehyde are
} used for fluorescnce microscopy, and the Karnofsky fixed samples for TEM.
} Thanks in advance for your experience/advice.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}


From daemon Tue Jun 26 10:27:39 2001



From: Rachel Spicer :      spicer-at-oeb.harvard.edu
Date: Tue, 26 Jun 2001 11:25:19 -0400
Subject: thanks re: long term storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to my post re: long term storage of plant
material in fixatives. The responses were as varied as the recommendations
in the literature, but I find there is no substitute for hearing from those
with first hand experience. If anyone is interested in the specifics I'd
be happy to forward a collection of relevant responses. And finally,
apologies for my misspelling of "Karnovsky". Thanks again.

Rachel

******************************************
Rachel Spicer
Biological Laboratories 3119
Organismic and Evolutionary Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

(617) 496-3580 (phone)
(617) 496-5854 (fax)
spicer-at-oeb.harvard.edu
******************************************



From daemon Tue Jun 26 12:05:04 2001



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Tue, 26 Jun 2001 12:32:23 -0500
Subject: imaging plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon -

Congratulations on your E3 acquisition - they're generally easy-running
instruments once you get the preliminary bugs out.
I've been running an older E3 for the past few years, but that doesn't
make me an expert on them. Your vacuum problems sound a little funny - have
you checked that both diff pumps are hot to the touch? If one is cold (the
one on the right hand side of the column, viewed from the front, that could
explain part of the problem. If it is, try the reset button on its "front"
side, and check cooling water flow to make sure it's adequate. Somebody good
with electrical stuff (I'm not) can probably determine whether or not your
diff pump element is still good.
We once had V1 stick a bit, obstructing the beam, but were able to free
it up by reversing the compressed air flow momentarily. You can actually
watch it open and close if you remove the sheet-metal shroud on the left
(the one with the black cylindrical receptacle for the gun for those times
when you've pulled the gun - I know, I thought it was a cup holder at first,
too, but believe me, it isn't...)
The E3 is still supported by FEI, though, and I've always found their
guys pretty good about being able to fix things over the phone, even without
a service contract. You can get their phone numbers, etc from the site
www.feicompany.com. The guys in the Peabody, Mass. office should be able to
help you through this one - that's where your E3 was born.
Hope this helps.....

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada


----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.Berkeley.EDU}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, June 25, 2001 7:11 PM


Julian asked if imaging plates are “as good as negatives”. There are
advantages and disadvantages to all imaging systems. In some instances
CCD cameras are preferable to film, in other instances film is superior
(especially if wide field of view is a factor). What is best to use
largely depends on the details of your experiment. I thought I would
provide some information on imaging plate systems, and indicate one
strong advantage the technology has over other recording media/systems.

Image plate systems use the "photostimulated luminescence" (PSL)
phenomenon. Regions in PSL materials that have been previously exposed
to a short wave length irradiation, will luminesce when stimulated by a
second longer wavelength irradiation. The stored energy is stable until
released by the second irradiation. Imaging plates are made using PSL
films of barium fluorobromide (= 5 µm grain size), containing a trace
amount of bivalent europium as a luminescence center, which are
uniformly coated on a polyester support. Imaging plates replace
conventional photoemulsion film in the transmission electron microscope
and are exposed the same as normal film. To read the recorded image,
the imaging plate is latter scanned by a red diode laser and the
luminescence is collected through a light collection guide to the
photo-multiplier tube (PMT). The analog current produced by the PMT is
converted to a 8 to 16 bit depth digital signal. The pixel density can
be read at between 50 to 400 pixels/cm. Imaging plates can be fully
erased for reuse, but have a finite lifetime.

One main advantage of PSL films in their ultrahigh sensitivity (e.g. 2
x 10-14 - 2 x 10-9 C-cm-2 at 100 kV) to electron irradiation making them
valuable for biological work (these are values quoted by a manufacturer
Fuji). For example, film emulsions require 0.1 to 1 C-cm-2 to record an
optical density S = 1 at 100 k magnification (see Reimer, 1975). Slow
scan CCD cameras offer better sensitivity than film, however are
inadequate for imaging certain biological specimens.  Furthermore,
CCD cameras have limited fields of view as compared to film. As an
example, Sugi (1997) and coworkers have used a Fuji imaging plate system
to record micrographs of myosin head movement induced by ATP hydrolysis
in an environmental cell TEM. I avoided using the term “living myosin”
since a biomolecule is not strictly alive, but it was biologically
active. Electron doses low enough not to damage the delicate
biomolecule could be used to record images on an imaging plate.

Reimer, L. (1975) in Physical Aspects of Electron Microscopy and
Microbeam Analysis, eds B. M. Siegel, and D. R. Beaman, John Wiley &
Sons Publishers, New York, New York, 231-245.

Sugi, H., Akimoto, T., Sutoh, K., Chaen, S., Oishi, N., and Suzuki, S.
(1997) Proc. Natl. Acad. Sci. USA 94, 4378-4382.


} } } Does anybody have experience with High Resolution Imaging Plate
Scanner
} for
} } } Electron Microscopy (http://www.ditabis.de). Instead of
conventional
} } } EM-films, flexible photostimulable phosphor screens of the same
size, the
} } } so-called Imaging Plates, are loaded into the TEM magazine. Are
they as
} } } good as negatives?
} } }
} } } Thanks,
} } } Julian
} } }
} } } _______________________________
} } } Julián Martínez Fernández
} } } Senior Research Associate
} } } MS 106-5 Ceramic Branch
} } } NASA Glenn Research Center
} } } Cleveland, OH 44135
} } } Phone: 1-216-433-5512
} } } Fax: 1-216-433-5544
--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Tue Jun 26 15:27:51 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Mon, 25 Jun 2001 15:28:29 -0700
Subject: RE: TEM nanoparticle contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Thanks so much Charles. I just wonder why I did not know that such grids are
available. I use JEOL 200CX TEM to look at nanoparticles. What is the
thinnest window grid do you recommend?

Regards,

Thearith

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --



This has become quite a generic problem for many who are working with nano
size particles, and many have found the "solution" to be the use of silicon
nitride membrane window grids that have been offered by SPI Supplies for
several years. Information about these grids can be found on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html

The silicon nitride is completely structureless and featureless and seems to
provide excellent electron transparency. The other advantage is that they
are sufficiently robust to survive temperatures in excess of 1000° C. And
there is no carbon grain to be confused with the nanoparticles of interest.

Disclaimer: SPI Supplies offers silicon nitride membrane window grids for
this type of application.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Tue Jun 26 16:15:32 2001



From: Lou Bustillos :      lbustillos-at-amalab.com
Date: Tue, 26 Jun 2001 17:03:30 -0400
Subject: Looking for a "HAXI" kit for a JEOL TEM.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We are in the process of purchasing an EDS system for our JEOL 100 CX II
TEM microscope. We would like to have the EDS system installed at a high
angle but we need a "HAXI" kit in order for the EDS system to be installed
correctly.

Does anyone have a "HAXI" kit for purchase?

Thank you,

Lou Bustillos
AMA Analytical Services, Inc.
lbustillos-at-amalab.com





From daemon Tue Jun 26 17:08:54 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Tue, 26 Jun 2001 18:01:58 -0400
Subject: imaging plates in the physical sciences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Image plate system is awesome for materials applications as well.... !!

It is very easy to obtain diffraction patterns that include varying
intensities (e.g., a spot pattern mixed with Kikuchi lines) with just one
exposure on a single plate. The plates also show the slightest deviations
in contrast which are not discernable using film, a CCD camera, or the TEM
viewing screen. For example, differences in contrast in amorphous silicon
oxide due to the multiple processing steps in integrated circuits may be
easily observed with the plates. The advantage in the plates is evident in
the low to intermediate magnification ranges where large variations in
image contrast can be manipulated off-line. Each IP file is quite large
(25MB) and therefore, contains plenty of information which can be
manipulated before reducing its size that is suitable for publication
purposes. The disadvantages to the IPs are the cost (although I believe
the cost is dropping) and the extra processing time needed (~ 80 minutes
for a batch of 32 plates).

The CCD camera is still very useful in the high resolution imaging regime
where the intensity is rather uniform throughout the field of view and one
can instantly observe the quality of the image. We will be evaluating high
resolution CCD images with high resolution images obtained with the IPs in
the near future. We are not sure what effect (if any) vibrations from the
film mechanism will have on the high resolution images.

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Tue Jun 26 21:08:23 2001



From: Paul Webster :      pwebster-at-hei.org
Date: Tue, 26 Jun 2001 18:56:50 -0700
Subject: Re: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

The messages from Professor Monson in reply to a question about long-term
tissue storage makes me wonder what has happened to this listserver?

His message was highly entertaining and even if the poster of the original
message may have felt "picked on", there was no reason to expect him to post
an apology, no matter how informative it was.

Far too many posters use this listserver as a way of finding a quick fix to
simple solutions to technical problems that can be easily researched in text
books.

Often these messages contain little background information on the technical
problem under investigation. Even so, many of these questions get full
attention and detailed replies, even if the replies are not posted on the
main list for public view.

What has happened to the free and open discussion of issues and protocols
that we used to have? Are people so afraid of being accused of being
incorrect that they do not post their replies openly? Or maybe people are
afraid of being accused of being impolite.

I look forward to controversy and hope that my comments are read carefully
and replied to with vigor. Only in this way will I be able to learn new
thing, and this is the main reason I still subscribe to this listserver.

If I am factually wrong about something, tell me. It is better to find this
out on the listserver than in a more professional (or cruel) environment.

As for discussion subjects: why was there no comment about the unadvisable
suggestion made about storing samples in a frozen state? Would anyone
working with EM really choose to store their samples at -20 degrees before
processing for TEM?

Where is all the discussion about extraction that can occur when storing
samples in alcohols or buffers, or on the contamination that I know grows in
everyones old cacodylate buffers?

Do we not comment because we know it has all been published in textbooks?

Dear Profesor Monson, do not feel bad about improving our general knowledge
and our professional etiquette. I for one support your views and find your
comments useful and entertaining. Please keep up your submissions.

Best regards,

Paul Webster


Paul Webster, Ph.D.
Scientist II and Director
Ahmanson Center for Advanced EM & Imaging
House Ear Institute
2100 West 3rd Street
Los Angeles
CA 90057

pwebster-at-hei.org
p: 213 273 8026
f: 213 413 6739
http://www.hei.org/research/depts/aemi/aemi.htm







From daemon Tue Jun 26 22:06:01 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 26 Jun 2001 23:00:37 -0500
Subject: Rare earth standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Scotty Cornelius wrote:
============================================
In a far corner of my mind, I vaguely remember a set of rare earth glass
standards that had been developed and were being offered for sale a few
years ago, possibly out of the UK. Does anyone know of such a set, or was
it all a pipe dream? I'm not referring to the Drake and Weill standards
from the early '70s.
================================================
Could you have in mind our (e.g. SPI Supplies) "15 Rare Earth 'Five
Phosphates' Standards" for Microanalysis? They can be found on URL
http://www.2spi.com/catalog/standards/reepo/reepo.html

At one point, we had inadvertently listed these as "glasses" when in fact
they are crystals. So we might have been ourselves the cause of some
confusion.

Chuck

Disclaimer: SPI Supplies offers a range of standards for microanalysis
including this unique rare earth "five phosphate" mount.

===============================================Charles A. Garber, Ph. D.
Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us!
############################
WWW: http://www.2spi.com
############################
===============================================


From daemon Wed Jun 27 00:49:17 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Wed, 27 Jun 2001 15:41:15 +1000
Subject: Re: High Resolution Imaging Plate Scanner for Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have just been testing the Ditabis IP scanner and were impressed.

It doesnt quite replace film or I think a good small-pixel below-screen CCD camera for resolution, but is much better than a whole field above screen CCD camera for resolution, and for dynamic range is in a class by itself.

We tested with diffraction patterns showing bright and dim diffuse features, convergent beam patterns, wedge-profile ion beam thinned sections, and normal and unstained biological material.

For low dose work, 1-2 seconds exposure at beam intensities barely visible to the eye was no problem. The resolution problem may be avoidable in practice because images may be taken at higher magnifications and lower beam intensities than normal for film - that is just a question of getting used to matching the imaging conditions to a different medium.

PLate handling is easy. The plates are loaded into the TEM magazine in the light. AFter electron exposure they are kept dark, but loaded into the scanner under light as dim as convenient - we used the light from the monitor screen.

Storing the plates for up to five days made only a very small difference to the intensity histogram - we didnt test beyond that. Plates can also be rescanned with very little loss.

The only real inconvienience we found was the impossibility of recording plate number, mag etc on the IP plate, as we are used to on the negative. If we had our own system we would mark the plates permanently in some way that showed on the readout.

IP plates are complementary to CCD cameras in some respects. You dont get the advantages of immediate viewing, but more dynamic range, more pixels, and of course one scanner can take plates from any number of TEMs.
Finally, the price was the sticking point for us at this time. But if a cooperative lab somewhere else on this continent gets one we would likely buy a set of plates and work through the postal system until the price drops or somebody comes up with a "must have" application!

regards
Sally Stowe


Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU



} } } Julian Martinez-Fernandez {Julian.Martinez-Fernandez-at-grc.nasa.gov} 06/26/01 05:11AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anybody have experience with High Resolution Imaging Plate Scanner for
Electron Microscopy (http://www.ditabis.de). Instead of conventional
EM-films, flexible photostimulable phosphor screens of the same size, the
so-called Imaging Plates, are loaded into the TEM magazine. Are they as
good as negatives?

Thanks,
Julian

_______________________________
Julián Martínez Fernández
Senior Research Associate
MS 106-5 Ceramic Branch
NASA Glenn Research Center
Cleveland, OH 44135
Phone: 1-216-433-5512
Fax: 1-216-433-5544





From daemon Wed Jun 27 08:01:53 2001



From: Nora Pratta- Silvia Montoro :      csedax-at-ceride.gov.ar
Date: Wed, 27 Jun 2001 09:58:08 -0300
Subject: Printing greyscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have downloaded from Lumijet4s home page information about
the Preservation Monochrome Inks they commercialize for jetink printers, a
sort of special ink for good image tonality and permanence. Has any one had
any experience with that product? or any other like that?

Regular black ink cartridge would be enough por good quality greyscale
printing???

Thanks a lot,

Silvia Montoro
Centro Regional de Investigacisn y Desarrollo de Santa Fe (CERIDE)
G|emes 3450
3000 Santa Fe



From daemon Wed Jun 27 08:51:21 2001



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Wed, 27 Jun 2001 08:47:24 -0500
Subject: ESEM E3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We also have an E3 and have had our share of vacuum problems. Last year the
vacuum had gotten to the point that we needed to do something. Another
employee and I took all the valves off and removed the diffusion pumps. We
cleaned up the pumps and added oil. Then we replaced all the o-rings in the
couplings and valves and diff pumps. (basically they were all flat) After
that the vacuum was as good as when we first got it and pumping down was
much faster. This is something that probably should be done every 10 years
whether it needs it or not!
We also had some mysterious water loss from the chiller for over a year that
finally appeared as a major water leak at the diff pump tubing. We cut off
the brittle parts and reconnected. Cooling was better and vacuum too.
Of course you should be sure all your vacuum pumps are up to the job,
particularly the large pump used for the column. The vacuum hose that goes
between the 2 diff pumps should have a metal cover on it to protect it from
the heat. It does become brittle and should be checked for leaks. We
replaced ours as well.
As others have mentioned, the E3 is still supported and although we also do
not have a service contract, the engineers at FEI versed in the E3 are VERY
helpful, patient and willing to troubleshoot a problem over the phone! Good
luck!
Regards,
Mike
********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************


From daemon Wed Jun 27 09:46:09 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 27 Jun 2001 09:45:06 -0500
Subject: Hitachi S-570 LaB6 crystal & Wehnelt

Contents Retrieved from Microscopy Listserver Archives
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If anyone has a Hitachi S-570 with LaB6 that they are getting rid of,
we would be interested in any left-over LaB6 crystals mounted in the
Wehnelts. Thanks!

Probably today's humor, but hey, it's worth a try.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Jun 27 10:09:37 2001



From: jfb :      jfb-at-uidaho.edu
Date: Wed, 27 Jun 2001 08:03:22 -0700
Subject: Re: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,
While you are correct in stating that the answers to many of the
questions asked on this listserver are easily found in a number of
available texts, many of the libraries at smaller colleges and
universities do not have the funds to maintain an extensive section
dedicated to electron microscopy or even microscopy in general.
Therefore, those books could be days away via inter-library loan. I
always looked at this server as a gathering of some of the great minds
in microscopy and a "mother lode" of information based purely on
experience, if nothing else. Being a one man show at a small
university, I am constantly bombarded with questions about various
techniques from investigators throughout the sciences, and have utilized
this source many time for answers to those inquiries. There are no
stupid questions, only ignorant people asking those questions; and
intelligent, informed and timely answers will make those folks somewhat
less ignorant.
I am now getting down from the soapbox.

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204



From daemon Wed Jun 27 10:18:22 2001



From: Xudong Fan :      fanx-at-msu.edu
Date: Wed, 27 Jun 2001 11:16:22 -0400
Subject: Philips CM-10 TEM available now

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our Philips CM-10 transmission electron microscope is now for sale by
Michigan State University.   For more details, please check the MSU
surplus web site:

http://surplus.msu.edu/frames/sales/bid/bids05.htm

If you have any other questions, please contact me at:
Ph: 517-353-4525. Fax: 517-4325174, Email: fanx-at-msu.edu,
We are available for instrument demonstration and examination
at any time.

Yours Sincerely,
Xudong Fan, Ph.D.
Center for Advanced Microscopy
Michigan State University
East Lansing, MI, 48824







From daemon Wed Jun 27 10:46:32 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 27 Jun 2001 11:41:11 -0400
Subject: RE: thanks re: long term storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rachel,
Let me add.
1. On storage in buffer: NOT recommended by all my
experience and all who have ever advised me.
2. On storage in fixative: NOT recommended as above,
always because of unknown variables.
3. On fluorescence microscopy after HCHO: HCHO (dry)
has been used to induce fluorescence in so-called biogenic amines (incl.,
decarboxylated amino acids). One never knows what else it may do during
long residence. Ask yourself: Do you want fluorescence of fixed wood or
wood?
4. One cannot fix with paraformaldehyde (not your
fault). It is a polymer of HCHO and must be depolymerized to yield full
methanalic (arch.) power! This (depolymerization) can occur when
paraformaldehyde is suspended in a neutral or slightly basic buffer and
permitted to stand for a while or heated briefly to 50-60degreesC.

The real identity of paraformaldehyde was the only thing I remember
from my failed first course in freight-train organic chemistry [A carload of
this and a carload of that yields half a carload of something else!]. A
second foray into mechanistic organic went much better.

Summary: Old-timers stored materials to save information.
Young folks can't afford the luxury if they want to collect data.

Moral: If you can have some tea while he vents, you might
survive to get some solid advice.

Final Regards,

Fred Monson,



} ----------
} From: Rachel Spicer
} Sent: Tuesday, June 26, 2001 11:25 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: thanks re: long term storage
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks to all who responded to my post re: long term storage of plant
} material in fixatives. The responses were as varied as the recommendations
} in the literature, but I find there is no substitute for hearing from
} those
} with first hand experience. If anyone is interested in the specifics I'd
} be happy to forward a collection of relevant responses. And finally,
} apologies for my misspelling of "Karnovsky". Thanks again.
}
} Rachel
}
} ******************************************
} Rachel Spicer
} Biological Laboratories 3119
} Organismic and Evolutionary Biology
} Harvard University
} 16 Divinity Avenue
} Cambridge, MA 02138
}
} (617) 496-3580 (phone)
} (617) 496-5854 (fax)
} spicer-at-oeb.harvard.edu
} ******************************************
}
}
}


From daemon Wed Jun 27 10:51:11 2001



From: NPGSlithography-at-aol.com
Date: Wed, 27 Jun 2001 11:47:03 EDT
Subject: Re: EM Service vs Manufacturers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A list of independent EM service providers can be found at
"www.jcnabity.com/service.htm".

Note that their "service areas" are not necessarily limited to the "location"
that is listed.

If anyone has additional information that can be included on this list,
please let me know. Any suggestions for independent EM service providers
that are based overseas will also be appreciated.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com

In a message dated 6/25/2001 2:59:20 PM Mountain Daylight Time,
verlaj-at-medicine.ufl.edu writes:

} Beverly Giammara wrote asking for comments about EM service providers,
} other than the manufacturers, that cover the Midwest. I would be very
} appreciative if anyone can offer similar information about EM Service
} providers that cover Florida.
}
} Sincerely,
}
} Jill
}
} Jill Verlander Reed, D.V.M.
} Associate Scientist
} Director, College of Medicine Electron Microscopy Core Facility
} University of Florida
} P.O. Box 100215
} Gainesville, FL 32610
} verlaj-at-medicine.ufl.edu
} Phone: (352) 846-0820
} Fax: (352) 846-3299
}




From daemon Wed Jun 27 11:00:42 2001



From: Shea Miller :      millers-at-em.agr.ca
Date: Wed, 27 Jun 2001 12:00:23 -0400
Subject: immunofluorescence on plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone:
I have a student who wants to do some immunolocalization of a protein in plant tissue. I have been combing my files, but haven't managed to find a decent protocol that she could work from... (and can't seem to get myself to the library for a more comprehensive search). Can anyone supply us with a "generic" protocol for immunofluorescence in plants?

Thanks so much in advance
shea





From daemon Wed Jun 27 12:33:01 2001



From: Smartech :      smartech-at-optonline.net
Date: Wed, 27 Jun 2001 13:32:56 -0400
Subject: Metallurgy, Weld Failure Analysis, SEM Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A customer of mine sent a part that has started showing a failure only
recently, although he has manufactured the part for years. The part is a
heating element. It consists of a thick SS rod which is the terminal. It
has a diameter of about 1 cm. It is joined to an element that is about 2 mm
in diameter. The joint is made by 1st drilling the thick stock and then
inserting the wire into the hole. Then a notch is made in the thick stock
until the thin element is exposed. Then the notched surface is welded
without the introduction of any new material. I believe it is a "TIG" weld.
A standardless analysis of the terminal shows Fe (82%), Cr(13%), Al(3%),
Si(1.6%), and Mn (.3%). He has tried to match the two materials as closely
as possible.

The joint is heated repeatedly, as it is used as an element in a glass
furnace. The failure starts with a bulk flow of material out the top of the
hole, which is about 1.5-2.0 cm from the welded joint/notch, and over the
sides of the terminal. I have analyzed this material and it contains
principally Fe and O. The area I analyzed was polished so the internal
composition of the flowed material contains a great deal of oxygen. No Cr
is detected and very little Al.

I feel that this material flow degrades the joint causing the various
electrical failures that have been observed.

My questions are:

Is this a typical failure mode with a Fe/Cr SS weld experiencing many heat
cycles?

What is the mechanism that results with the crystalline appearance of the
iron oxide, leaving the other elements behind?

This failures appears to have started when a new aqueous lubricant was
introduced for the initial hole drilling of the terminal. Could this play a
role in the failure?

What measures might be taken to reduce the frequency of this failure mode?
Joint design? Material compatibility?


Thanks in advance!

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Jun 27 15:19:27 2001



From: Ping Li :      pli-at-is.dal.ca
Date: Wed, 27 Jun 2001 17:10:28 -0300
Subject: Re: sharpening diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, could anyone please give me some information on where I can get my
diamond knives (for semi thin sectioning) sharpened? Any such places in
Canada? I believe the knives were made in Germany. Any information is
highly appreciated. Thank you in advance.

Ping



From daemon Wed Jun 27 15:46:17 2001



From: Jon Hiller :      hiller-at-anl.gov
Date: Wed, 27 Jun 2001 15:41:25 -0500
Subject: FIB-need micromanipulator info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers,

I would like to gather some information from the micromanipulator
community. Specifically, this tool will be used for lamella extraction
after the FIB work is done. We will be in the market to purchase one in the
upcoming months so I'm starting my homework early. Ideally, this will be a
dedicated station with an anti vibration table along with the optical
microscope. Some questions are:
1) Motorized vs manual
2) Objective lens working distance
3) Probe resolution
4) Sensitivity (microns per degree)

Any comments or suggestions are welcomed!

Thank you,

Jon Hiller

==================================================================
Jon M. Hiller
Argonne National Laboratory
Materials Science Division
Electron Microscopy Center
Tel: 630-252-9558
Fax: 630-252-4798
Email: hiller-at-anl.gov
==================================================================



From daemon Wed Jun 27 16:26:52 2001



From: sghoshro-at-NMSU.Edu
Date: Wed, 27 Jun 2001 15:20:05 -0600 (MDT)
Subject: Re: immunofluorescence on plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shea,

There is a wonderful protocol for immunofluorescence in a manual which I
use for most of my Arabidopsis work.

Methods in Plant Molecular Biology
A laboratory course manual
Maliga, Klessig, Cashmore, Gruissem, Varner
Cold Spring Harbor Lab Press

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml

On Wed, 27 Jun 2001, Shea Miller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone:
} I have a student who wants to do some immunolocalization of a protein in plant tissue. I have been combing my files, but haven't managed to find a decent protocol that she could work from... (and can't seem to get myself to the library for a more comprehensive search). Can anyone supply us with a "generic" protocol for immunofluorescence in plants?
}
} Thanks so much in advance
} shea
}
}
}
}
}



From daemon Wed Jun 27 16:32:21 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 27 Jun 2001 16:16:20 -0500
Subject: RE: Metallurgy, Weld Failure Analysis, SEM Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Ric,

What is the composition of the heating element? The S.Steel terminal is not
a common (to me) stainless, given the composition you obtained. I would
have to check references to see what it may match. I work mostly with
austenitic stainless and nickel super alloys when it comes to "SS".

I suspect the welling up of oxide from the joint/hole is a symptom rather
than a cause of the failure. Contact area and/or conductor size reduction
will (all else being the same) cause a hot spot. What is the shape of the
FeOxide crystals? Sounds like you may have magnetite, a stochiometry often
formed under oxygen starved (but still oxidizing) environment.

I would question the customer carefully about process conditions which
result in failure. What is the composition of the lube? If residue
remains, it could surely play a role in the corrosion. Are there any
differences in glass chemistry or temperatures compared to the non-failed
parts? What environment is "seen" by the joint? -} In the Glass, near it?
Glass chemistry? If near it, what is the atmosphere? Temperatures? Heating
power/time curve the same (i.e. any localized I2R heating of joint)? Getting
accurate and complete information from customers is sometimes the most
difficult part of failure analysis. ...Especially if it is second hand.

Too much weld heat can "sensitize" some alloys (changes carbon/carbides),
reducing resistance to corrosives. The failure mode, however, is most often
intergranular cracking and (again) I am not familiar with the
characteristics of your composition.

So many questions, so few answers, sorry!

Woody


} -----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
} Sent: Wednesday, June 27, 2001 1:33 PM
} To: To all on the list
} Subject: Metallurgy, Weld Failure Analysis, SEM Analysis
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} A customer of mine sent a part that has started showing a failure only
} recently, although he has manufactured the part for years.
} The part is a
} heating element. It consists of a thick SS rod which is the
} terminal. It
} has a diameter of about 1 cm. It is joined to an element
} that is about 2 mm
} in diameter. The joint is made by 1st drilling the thick
} stock and then
} inserting the wire into the hole. Then a notch is made in
} the thick stock
} until the thin element is exposed. Then the notched surface is welded
} without the introduction of any new material. I believe it
} is a "TIG" weld.
} A standardless analysis of the terminal shows Fe (82%),
} Cr(13%), Al(3%),
} Si(1.6%), and Mn (.3%). He has tried to match the two
} materials as closely
} as possible.
}
} The joint is heated repeatedly, as it is used as an element in a glass
} furnace. The failure starts with a bulk flow of material out
} the top of the
} hole, which is about 1.5-2.0 cm from the welded joint/notch,
} and over the
} sides of the terminal. I have analyzed this material and it contains
} principally Fe and O. The area I analyzed was polished so
} the internal
} composition of the flowed material contains a great deal of
} oxygen. No Cr
} is detected and very little Al.
}
} I feel that this material flow degrades the joint causing the various
} electrical failures that have been observed.
}
} My questions are:
}
} Is this a typical failure mode with a Fe/Cr SS weld
} experiencing many heat
} cycles?
}
} What is the mechanism that results with the crystalline
} appearance of the
} iron oxide, leaving the other elements behind?
}
} This failures appears to have started when a new aqueous lubricant was
} introduced for the initial hole drilling of the terminal.
} Could this play a
} role in the failure?
}
} What measures might be taken to reduce the frequency of this
} failure mode?
} Joint design? Material compatibility?
}
}
} Thanks in advance!
}
} Ric
}
} SMARTech
} 860-491-3299
} www.semguy.com
} 19 Cornwall Drive
} Goshen CT 06756
}
}


From daemon Wed Jun 27 17:47:57 2001



From: Christine Edly :      cedly-at-ou.edu
Date: Wed, 27 Jun 2001 17:42:41 -0500
Subject: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings!

I'm in search of something that will remove immersion oil from slides of

blood smears (no coverslips) without damaging the specimens. A friend
told that such a chemical exists, but could not remember the name. It
supposedly smells like oranges, and xylene was once used for the same
purpose. If you have any ideas as to the name of this mystery chemical,

I'd sure appreciate it.

Regards,
Christine Edly
University of Oklahoma


From daemon Wed Jun 27 23:32:15 2001



From: Ingrid R. Niesman :      iniesman-at-ixpres.com
Date: Wed, 27 Jun 2001 21:20:19 -0700
Subject: nitrocellulose filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know the best method for embedding 0.45um nitrocellose filter for TEM
thin sectioning? We have been filtering vesicle preparations onto these filters and
wish to examine them by TEM analysis for immunolabelling. Our 2 previous attempts
with Epon mixtures and minimal intermediate solvent changes have lead to poorly
polymerized blocks and dissolving membranes. Currently we are trying LR White as a
substitute. Any help would be great.
Thank you.
Ingrid Niesman
SRA III Farquhar/Palade Lab
UCSD


From daemon Thu Jun 28 03:04:26 2001



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Thu, 28 Jun 2001 09:56:16 +0200
Subject: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Is the DTSA (Desk Top Spectrum Analyzer) package available for PC or only
for Mac? I looked on the NIST web site but couldn't find information (and
their internal search engine was broken).

Thanks for any leads you can give.

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/



From daemon Thu Jun 28 06:04:32 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 28 Jun 2001 11:53:49 +0100 (GMT Daylight Time)
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have no idea if it works for your purpose, but the
xylene substitute smelling of oranges is called Histoclear.

Dave


On Wed, 27 Jun 2001 17:42:41 -0500 Christine Edly
{cedly-at-ou.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} I'm in search of something that will remove immersion oil from slides of
}
} blood smears (no coverslips) without damaging the specimens. A friend
} told that such a chemical exists, but could not remember the name. It
} supposedly smells like oranges, and xylene was once used for the same
} purpose. If you have any ideas as to the name of this mystery chemical,
}
} I'd sure appreciate it.
}
} Regards,
} Christine Edly
} University of Oklahoma
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Jun 28 06:46:08 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 28 Jun 2001 13:45:11 +0200
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I have used the 'orangey' smelling solvent under the brandname of
Histoclear. It is supposedly extracted large scale from citrus peel and is
the composed of the 'zest' that comes out as a spray when we peel oranges.

I couldn't comment on its use for removing immersion oil as you describe. I
have only used it as a xylene substitute for tissue processing tissues and
also for clearing stained sections prior to mounting.

It is said to be safer than xylene but some of the people I worked with at
the time complained of headaches so we stopped using it despite never
proving that it was the cause of the headaches.

Hope this helps!



At 17:42 2001-06-27 -0500, Christine Edly wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu Jun 28 07:13:44 2001



From: Sousan Abolhassani :      sousan.abolhassani-at-psi.ch
Date: Thu, 28 Jun 2001 14:07:28 +0200
Subject: Re: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

The point about this server is that it is used by
a very heterogeneous public from which a non-exclusive
list is the following:

students,
professors,
scientists/
researchers
technical staffs
suppliers
producers
specialists
non-specialists
etc.
etc.
etc.

and all these from so many different backgrounds with so
many different problems and even from so many different countries...
That if we want to teach all of them to behave as we expect
before they post a query, the server could go to early retirement.

I guess the philosophical attitude that one should have is to
really try and understand both sides of the discussion.

1- on one hand the trust that professor Monson is demanding
is not there because our users are a small sample of our
society. How many times have we heard that people have had
their ideas picked by others and their publications stolen
by others and .... in the very scientific community that most
of us hoped to be the purest part of the society. Still
the scientists are generally speaking very sincere people.
So if someone is not having trust to others, it's the
consequence of what they have observed in their environment.

2- I do not know if Rachel is a student (may be not), and if she
really was not trusting or just did not think to write the reason and
so on, so we do not discuss the case on a specific way.
But talking about going to the library, there are labs
where the students have really not access to the books and there
are places where superiors do not give the support the students
and other researchers' need, or simply there are not
enough books available.

So there are always reasons for the way people behave
and the more our society goes towards a very materialistic and
"win whatever the situation" attitude, the more the arguments
of professor Manson will be applicable because the more people
will behave in the way he is not happy about, and I understand
and apreciate his point.

All that apart, it is better to listen to people,as often things
that are not to be done are not published and are only "known".
Of course provided that answers are given generously, as arrogance
for giving is so destructive, even for donator.
I therefore agree with the fact that we should just be able to
ask the question and someone will react to give us an answer.

I think that professor Monson has evoked a point that is of
great importance and that is the human aspect of all we do, as
"human" is what we are hoping to be at a first place.
That needs sensitivity... and continuous effort.

Regards,

Sousan


Paul Webster wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Colleagues,
}
} The messages from Professor Monson in reply to a question about long-term
} tissue storage makes me wonder what has happened to this listserver?
}
} His message was highly entertaining and even if the poster of the original
} message may have felt "picked on", there was no reason to expect him to post
} an apology, no matter how informative it was.
}
} Far too many posters use this listserver as a way of finding a quick fix to
} simple solutions to technical problems that can be easily researched in text
} books.
}
} Often these messages contain little background information on the technical
} problem under investigation. Even so, many of these questions get full
} attention and detailed replies, even if the replies are not posted on the
} main list for public view.
}
} What has happened to the free and open discussion of issues and protocols
} that we used to have? Are people so afraid of being accused of being
} incorrect that they do not post their replies openly? Or maybe people are
} afraid of being accused of being impolite.
}
} I look forward to controversy and hope that my comments are read carefully
} and replied to with vigor. Only in this way will I be able to learn new
} thing, and this is the main reason I still subscribe to this listserver.
}
} If I am factually wrong about something, tell me. It is better to find this
} out on the listserver than in a more professional (or cruel) environment.
}
} As for discussion subjects: why was there no comment about the unadvisable
} suggestion made about storing samples in a frozen state? Would anyone
} working with EM really choose to store their samples at -20 degrees before
} processing for TEM?
}
} Where is all the discussion about extraction that can occur when storing
} samples in alcohols or buffers, or on the contamination that I know grows in
} everyones old cacodylate buffers?
}
} Do we not comment because we know it has all been published in textbooks?
}
} Dear Profesor Monson, do not feel bad about improving our general knowledge
} and our professional etiquette. I for one support your views and find your
} comments useful and entertaining. Please keep up your submissions.
}
} Best regards,
}
} Paul Webster
}
} Paul Webster, Ph.D.
} Scientist II and Director
} Ahmanson Center for Advanced EM & Imaging
} House Ear Institute
} 2100 West 3rd Street
} Los Angeles
} CA 90057
}
} pwebster-at-hei.org
} p: 213 273 8026
} f: 213 413 6739
} http://www.hei.org/research/depts/aemi/aemi.htm


From daemon Thu Jun 28 07:13:44 2001



From: Richard M Langford :      richard.langford-at-materials.oxford.ac.uk
Date: Thu, 28 Jun 2001 13:02:47 +0100
Subject: FIB micro-manipulator

Contents Retrieved from Microscopy Listserver Archives
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Dear Jon

The micromanipulator we use is a manual hydraulic system (Narishige MNN-1)
which is suitable for the plucking process. I do not know how its
performance compares of to other makes.

We use a microscope with *50 and * 5, 18 mm working distance lenses.

A couple of general comments about the lift-out process which might be
useful.

We use both pulled glass needles and electro-polished metal needles. The
yield of plucking using these two types of needles is comparable. An
advantage of the metal needles is that they do not shatter if accidentally
knocked against the sides of the FIB cuts. An advantage of the glass needles
is that if a cross-section has moved up the needle's shank, away from the
tip, (which sometimes occurs) then it is possible to see it and therefore,
not waste time searching for it in the area surrounding the FIB cuts. (In
addition to riding up the needle's shank sometimes a cross-section can also
'flick ' out away from the cuts while it is being plucked).

For cross-sections that have moved up the shank it is possible to
re-position them at the needle's tip using a second micro-manipulator.
However, it is also possible to get these cross-sections off the needle and
onto the support TEM grid by gently tapping the micro-manipulator system
while the needle is positioned over the grid.

Instead of using the Formvar or carbon support grids we use copper grids
with 7 um sized holes. We have found that only very rarely does a
cross-section fall off these. The advantage of these is that there is no
support membrane. What can happen is that while a cross-section is being
placed onto the support membrane the support membrane can be torn by the tip
of the needle and it can then wrap itself around the cross-section.

Also, because of the diversity of use of FIB systems (e.g. for modification
or fabrication of optoelectronic devices and micromachining) a FIB
discussion list has been set up. The subscribing web page is located at:

http://users.ox.ac.uk/~rml/

Any information, for example details of relevant FIB pages, can be added to
the web page if emailed to me.

Regards

Richard

--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273672, Fax: +44 (0)1865 273789
email: richard.langford-at-materials.oxford.ac.uk
----------------------------------------------------------------------------



From daemon Thu Jun 28 07:19:26 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 28 Jun 2001 14:18:50 +0200
Subject: Re: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
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Colleagues

I agree wholeheartedly with Paul in his comments regarding the mails from
Frederick Monson - no need for apology, interesting and informative replies.

Higher education seems to be locked in an apparently paradoxical situation
with students being expected to 'take responsibility for their own
learning', the use of Student Centred & Problem Based Learning etc. The
paradoxical part is that they do not have the appropriate intellectual
tools to be able to carry out such assignments. Frustration with their lot
then spills out over tutors who are expected to take responsibility when
things go wrong (but mostly no feedback when things work).

Ask me a question and I will give you the most appropriate answer I can.
The answer may be wrong of course, possibly because I have an incorrect
understanding of the concepts involved, possibly because I was asked the
wrong question. If I respond to a question with a question of my own it is
because I am trying to seek clarification of what EXACTLY is being asked.
That, as I see it, is how we work in science.

Paul says - "If I am factually wrong about something, tell me. It is better
to find this out on the listserver than in a more professional (or cruel)
environment."

I agree absolutely here too - it is easier to fight tooth and claw here and
come to some agreement or compromise than if we are addressing 500 people
at a conference and one of them asks a simple question to which we have no
answer.

My appeal to those posing questions is to give as much background
information as possible and to respond clearly to questions on clarification.

My appeal to those answering is to identify clearly what is hearsay "in my
experience" "I got that straight from a guy in the pub last night".
Identify also the context of the answer/solution to the question/problem
and refer to published data whenever possible.

I look forward to a lively, ongoing debate (even though I am going for my
summer break tomorrow!).




Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu Jun 28 07:35:47 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 28 Jun 2001 14:34:49 +0200
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
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Hi again - supplementary information found by entering 'histoclear' as a
search term into

www.google.com

1. A supplier website there are US, UK and European links. Please note that
I am not connected with this company in any way!!

http://www.ralamb.net/tour/1.html

2. I just found the text below on

http://www.histosearch.com/histonet/Jan99A/Re.HematoxylinfadingandhiA.html
http://www.histosearch.com/

On Mon, 4 Jan 1999, Karen S Pawlowski wrote:

} My boss just noticed in the 1985 edition of Cellular Pathology Technique,
} Culling, Allison and Barr eds., that food oil derivatives, such as
} histoclear, will cause "haematoxylin" staining to rapidly fade. Is this
} true? I have been having problems with hematoxylin fading for quite some
} time and I use histoclear in my paramount all the time. 'Tho I began (8
} years ago) by using xylene, and I think those faded just as much.
}
} If histoclear does cause the stain to fade, are there any other
} substitutes for xylene that would work?

R. D. Lillie made extensive studies of mounting and fading
in the 1940s and 1950s (reported in Ch. 5 of his
"Histopathologic Technic" book, with references to papers
in Stain Technol).

Fading is typically due to either acidity or chemical reduction.
Canada balsam is acidic (abietic acid is a major component)
but this doesn't matter - the acid can't ionize in the
absence of water. Alum-haematoxylin stains keep for many years
in Canada balsam though an eosin counterstain may fade a little
over the course of 2 years. (This must be from reducing agents
in the medium.) Easily reduced colours fade in Balsam (e.g.
Prussian blue, acid fuchsine) and are more stable in synthetic
media. Easily oxidized colours (notably cobalt sulphide) are
well preserved in Canada balsam but fade in a few months in
most synthetic resins. (You can revive CoS by removing the
coverslip and exposing to ammonium sulphide again.)

Limonene (I think this is what histoclear is) is a reducing
agent - two non-conjugated double bonds in the molecule - so
its presence in a mounting medium is undesirable. Some is
bound to persist, just as some xylene persists when the
mounting medium dries. The boiling point of limonene (176C) is
a bit higher than xylene (140C), so it might be a bit more
persistent.

If your eosin faded equally after xylene or limonene the
mounting medium is the probable culprit. Try a wholly synthetic
(polystyrene or methacrylate) medium rather than a natural
or semi-synthetic one. Synthetic media are the best bet for
pretty well everything except cobalt sulphide (from ATPase
histochemistry etc). The blue component (alum-haematein) of
an H & E stain is very resilient and should not be affected
by clearing agents and mounting media. It keeps for decades
(perhaps for ever). If yours is fading, something acidic and
probably also some alcohol must be passing through into
the mounted preparations. This is surely unlikely to occur
on a regular basis!

Hope this helps. I'm sure I haven't thought of everything,
and have no personal experience with histoclear/limonene,
but you should be getting lots of other suggestions too.

John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1





Hi

I have used the 'orangey' smelling solvent under the brandname of
Histoclear. It is supposedly extracted large scale from citrus peel and is
the composed of the 'zest' that comes out as a spray when we peel oranges.

I couldn't comment on its use for removing immersion oil as you describe. I
have only used it as a xylene substitute for tissue processing tissues and
also for clearing stained sections prior to mounting.

It is said to be safer than xylene but some of the people I worked with at
the time complained of headaches so we stopped using it despite never
proving that it was the cause of the headaches.

Hope this helps!



At 17:42 2001-06-27 -0500, Christine Edly wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu Jun 28 07:42:36 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 28 Jun 2001 08:38:20 -0400
Subject: Re: sharpening diamond knives

Contents Retrieved from Microscopy Listserver Archives
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Ping,

It would be helpful to know what brand the knives are. Most knife
manufacturers will take back their own knives for sharpening at the factory.
Just find the local distributor for the brand of knife you have and they
will arrange things for you. Energy Beam Sciences is a U.S. Company. We
recommend and deal primarily with Diatome but we can also help with DDK.

Good luck,
Mike

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: Ping Li [mailto:pli-at-is.dal.ca]
Sent: Wednesday, June 27, 2001 4:10 PM
To: Microscopy list


Hi, could anyone please give me some information on where I can get my
diamond knives (for semi thin sectioning) sharpened? Any such places in
Canada? I believe the knives were made in Germany. Any information is
highly appreciated. Thank you in advance.

Ping





From daemon Thu Jun 28 08:09:21 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Thu, 28 Jun 2001 09:00:21 -0400
Subject: Printers and Paper - thanks

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

Just wanted to thank everyone who contributed to the discussion and suggested solutions for the problems we were having. It is very much appreciated - we have so many things to try and have learned a lot in the process.

My colleague, who shares the printer with me, was amazed, both with the range of answers and the rapidity of the responses - the perfect combination when you're having trouble in the lab. That's why I keep coming back to the group for answers and have stayed subscribed for so many years - there's no place like it!

Thanks again.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Jun 28 08:19:19 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 28 Jun 2001 09:15:22 -0400
Subject: Re: nitrocellulose filters

Contents Retrieved from Microscopy Listserver Archives
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When we do this sort of thing, we generally use Nuclepore filters, but I
think our trick should work for other filters as well. We keep the
material on the filters through fixation and change solutions with gentle
vacuum. Then we overlay the specimen with a thin layer of low gelling
point agarose, pull a gentle vacuum and then chill the filter to solidify
the agarose. The entire filter can then be submerged in the next fluid,
and the filter is peeled away from the agarose. If all goes well the
specimen should be trapped in the agarose and the filter material is no
longer a problem. Warning: do not fix agarose in glutaraldehyde. It does
not embed well.

At 09:20 PM 6/27/2001 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Ditrector, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Thu Jun 28 08:27:25 2001



From: MSA :      MSA-at-bostrom.com
Date: Thu, 28 Jun 2001 08:24:45 -0500
Subject: Microscopy & Microanalysis 2001- Upcoming Deadlines

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues:

This is a reminder concerning upcoming deadlines for the Microscopy &
Microanalysis 2001 meeting from August 5-9 in Long Beach, CA.

1) The deadline for hotel reservations at the meeting rate is this Friday,
June 29. The Westin still has some double rooms available for the entire
meeting period, but the other meeting hotels are booked. You can
reserve a room online by going to www.msa.microscopy.com and
following the links to M&M 2001 electronic forms.

2) The deadline for early meeting registration is Friday, July 6. Save
some money and register early! Electronic registration forms are also
available at the above site. I would also encourage those of you that are
interested to register for the Pre-meeting congress (only $10 more with
full meeting registration) and workshops ($120-$180) that are available.

3) The deadline for submitting a late breaking poster is Sunday, July
15th. If you would like to submit your work for this session contact Bob
Price by email at Price-at-med.sc.edu.

With nearly 700 presentations scheduled, a sold-out exhibit floor and an
excellent venue in Long Beach, California, M&M 2001 promises to be an
excellent meeting. On behalf of the MSA and MAS Councils, and the
Local Arrangements and Program Committees, I hope to see all of you
there.

Bob Price
M&M 2001 Program Chair


From daemon Thu Jun 28 08:45:09 2001



From: Sarka Lhotak :      lhotaks-at-mcmail.cis.mcmaster.ca
Date: Thu, 28 Jun 2001 09:40:16 -0400 (EDT)
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christine,
if it smells like oranges and is used instead of xylene it must be
Histoclear. I have mine from Electron Microscopy Sciences, called Xylene
substitute, Cat #23410-01. I have never used it for immersion oil removal
though (sounds like a good idea), I just use it to clean microtomes of
paraffin residue.

Sarka Lhotak
Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada
lhotaks-at-mcmaster.ca

} Greetings!
}
} I'm in search of something that will remove immersion oil from slides of
}
} blood smears (no coverslips) without damaging the specimens. A friend
} told that such a chemical exists, but could not remember the name. It
} supposedly smells like oranges, and xylene was once used for the same
} purpose. If you have any ideas as to the name of this mystery chemical,
}
} I'd sure appreciate it.
}
} Regards,
} Christine Edly
} University of Oklahoma
}
}



From daemon Thu Jun 28 09:21:10 2001



From: Yuhui_Xu :      Yuhui_Xu-at-hms.harvard.edu
Date: Thu, 28 Jun 2001 10:13:20 -0400
Subject: Freeze fracture system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues:

We are in the market for a freeze-fracture system. Currently there are two
models under our consideration: Bal-tec Freeze-Fracture Unit BAF060 and
RMC/JEOL 9010. We would appreciate any comments from users of these two
systems regarding to their applications, performance, stability, and other
features.

Thank you in advance.

Yuhui

Yuhui Xu, MD,PhD
EM Core, NHLBI, NIH



From daemon Thu Jun 28 09:29:10 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 28 Jun 2001 07:24:45 -0700 (PDT)
Subject: Re: nitrocellulose filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ingrid:
It has been a long time since I did this, but my notes show that we have
very good embedding using Epon, _but_ I have omitted propylene oxide from my
processing schedule (did that more due to contact allergies developed over
years of carelessness). Epon (and the various replacements are miscible
with EtOH--just make sure that you use freshly opened bottles. (Acetone is
another dehydrant that will tend to dissolve the filters, BTW.) Dehydration
steps can be shortened (through graded EtOH, including uranyl acetate in 70%
if you use a preembedding UA procedure), then go into 2:1, 1:1 and 1:2
EtOH:Epon for infiltration. I also use a minimum of 2 steps in pure before
embedding in fresh epoxy. I would guess that you are embedding in the flat
molds--best way for filters, IMHO. From personal experience, use of PO and
Spurr's are really out for the filters (and my recollection is the same for
acetone, as mentioned above). Hope it works out for you.

Roger

On Wed, 27 Jun 2001 21:20:19 -0700, Ingrid R. Niesman wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Does anyone know the best method for embedding 0.45um nitrocellose filter
for TEM
| thin sectioning? We have been filtering vesicle preparations onto these
filters and
| wish to examine them by TEM analysis for immunolabelling. Our 2 previous
attempts
| with Epon mixtures and minimal intermediate solvent changes have lead to
poorly
| polymerized blocks and dissolving membranes. Currently we are trying LR
White as a
| substitute. Any help would be great.
| Thank you.
| Ingrid Niesman
| SRA III Farquhar/Palade Lab
| UCSD
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Thu Jun 28 09:29:10 2001



From: Petersen, Maureen A. :      Mape-at-mail.ifas.ufl.edu
Date: Thu, 28 Jun 2001 10:24:53 -0400
Subject: LM- staining epoxy resin embedded sections for light microscopy

Contents Retrieved from Microscopy Listserver Archives
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I would appreciate any responses about the following situation:

I have epoxy resin embedded plant leaf (pepper) material infected with a
plant pathogenic bacteria (Xanthomonas vessicatoria). We plan to begin with
cutting 1-2 micron sections for light microscopy. I know that there are
stains using for differential staining of bacteria which are used on fresh
tissue or paraffin embedded material. Does any know of stains which would
work similarly for epoxy resin embedded material?

Thank you,

Maureen Petersen
Dept of Plant Pathology
University of Florida
Gainesville, FL




From daemon Thu Jun 28 10:39:12 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 28 Jun 2001 11:32:25 -0400
Subject: RE: nitrocellulose filters

Contents Retrieved from Microscopy Listserver Archives
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Fixation?
You shouldn't partially dehydrate or partially clear for EPON, but
assuming the membrane system is adequately preserved to survive the alcohol
and PropOx, then your problem is poor infiltration, due to poor clearing,
due to poor dehydration. This does not mean that you must dehydrate in
30min steps. The times can be reduced empirically until you get to the
minimum necessary times for dehydration and clearing. Lots of variables.
The filter should not be a problem in any case, though I have no practice
with isolated vesicles, only with isolated Eprythrozoon coccoides (a mouse
zoonose, something like a mycoplasma, likes RBC's, and 'hibernates') and
just morphology.
You might want to try GMA for TEM (e.g. SPI) though I have no
experience with it, or any other hydrophilic embedment.
I assume nano-gold, so you must have been into: Hayat, M.A.(1995),
Immunogold-Silver Staining, CRC Press, Boca Raton, FL, ISBN:
0-8493-2449-1(LC Card# 95-1831, LC Loc# QR187.I482H39). Chapter 4 by
Krenacs and Krenacs on the subject of: "Which embedding medium?" might be of
help.

Good hunting,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.

} ----------
} From: Ingrid R. Niesman
} Sent: Thursday, June 28, 2001 12:20 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: nitrocellulose filters
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone know the best method for embedding 0.45um nitrocellose filter
} for TEM
} thin sectioning? We have been filtering vesicle preparations onto these
} filters and
} wish to examine them by TEM analysis for immunolabelling. Our 2 previous
} attempts
} with Epon mixtures and minimal intermediate solvent changes have lead to
} poorly
} polymerized blocks and dissolving membranes. Currently we are trying LR
} White as a
} substitute. Any help would be great.
} Thank you.
} Ingrid Niesman
} SRA III Farquhar/Palade Lab
} UCSD
}
}


From daemon Thu Jun 28 11:49:40 2001



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 28 Jun 2001 13:41:27 -0300
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
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Listers -

This is very interesting, about the orange or citrus-based stuff that is
good for removing immersion oil. There is also a range of products sold for
de-greasing bicycle chains and derailleurs, which are based on citrus
components. I'm no organic chemist myself, but can one of the
chemistry-oriented people out there tell me why something citrus-based
should be so good at dispersing oils?
Call me curious.....and I hope I'm not too off-topic here..

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
B3L 4C8



From daemon Thu Jun 28 12:29:13 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 28 Jun 2001 13:23:59 -0400
Subject: RE: immunofluorescence on plants

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No Protocol, but this was a quick find on Google "Plant
immunohistochemistry"

Plant Cell Physiol. 37, 641-649 (1996): Developmental expression of a 23 kDa
jasmonate-induced protein (JIP-23) of barley leaves (Hordeum vulgare cv.
Salome) was studied by measuring the time-dependent accumulation of
transcript and protein during germination. Tissue-specific expression of
JIP-23 was analyzed immunocytochemically and by in situ hybridizations,
respectively. During seed germination JIP-23 mRNA was found to accumulate
transiently with a maximum at 32 h, whereas the protein was steadily
detectable after the onset of expression. The occurrence of new isoforms of
JIP-23 during germination in comparison to jasmonate-treated leaves
suggests, that the JIP-23 gene family of barley is able to express different
subsets of isoforms dependent on the developmental stage. JIP-23 and its
transcript were found mainly in the scutellum, the scutellar nodule and in
lower parts of the primary leaf of 6 days old seedlings. All these tissues
exhibited high levels of endogenous jasmonates. In situ hybridization
revealed specific accumulation of JIP-23 mRNA in companion cells of the
phloem in the nodule plate of the scutellum. In accordance with that, JIP-23
was detected immunocytochemically in phloem cells of the root as well as of
the scutellar nodule and in parenchymatic cells of the scutellum. The cell
type-specific occurrence of JIP-23 was restricted to cells, which are known
to be highly stressed osmotically by active solute transport. This
observation suggests, that the expression of this protein might be a
response to osmotic stress during development. URL:
http://www.ipb-halle.de/english/institute/research/hause/introduction.htm

Interesting,

Fred Monson

} ----------
} From: Shea Miller
} Sent: Wednesday, June 27, 2001 12:00 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: immunofluorescence on plants
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone:
} I have a student who wants to do some immunolocalization of a protein
} in plant tissue. I have been combing my files, but haven't managed to
} find a decent protocol that she could work from... (and can't seem to get
} myself to the library for a more comprehensive search). Can anyone supply
} us with a "generic" protocol for immunofluorescence in plants?
}
} Thanks so much in advance
} shea
}
}
}
}
}


From daemon Thu Jun 28 12:34:18 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 28 Jun 2001 13:30:04 -0400
Subject: Today's Real Humor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Question from an old exam given to students who demanded MORE MULTIPLE
CHOICE!

Which of the following is NOT involved with human reproduction?

a. transcription
b. replication
c. translation
d. osculation
e. none of the above

Answer tomorrow.



Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Thu Jun 28 12:39:44 2001



From: Paul Pederson :      pederso-at-US.ibm.com
Date: Thu, 28 Jun 2001 12:34:45 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please remove me from your mailing list

Paul Pederson,
IBM
Materials and Process Engineering
Integrated Engineering Solutions
Dept 5e3, Bldg 004-1
Rochester, MN 55901
Phone: 507 253 5661, t/l 553 5661
FAX: 507 253 4451
email: pederso-at-us.ibm.com



From daemon Thu Jun 28 14:47:36 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 28 Jun 2001 15:39:52 -0400
Subject: RE: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, MAC only,

Fred Monson

} ----------
} From: Ian MacLaren
} Reply To: Ian MacLaren
} Sent: Thursday, June 28, 2001 3:56 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: DTSA
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} Is the DTSA (Desk Top Spectrum Analyzer) package available for PC or only
} for Mac? I looked on the NIST web site but couldn't find information (and
} their internal search engine was broken).
}
} Thanks for any leads you can give.
}
} Ian MacLaren
} Max Planck Institut f¸r Metallforschung, Seestra­e 92,
} 70174 Stuttgart, Germany
} Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
} Home page: http://members.tripod.co.uk/IanMacLaren/
}
}
}


From daemon Thu Jun 28 15:20:49 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Thu, 28 Jun 2001 14:15:45 -0600 (MDT)
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please post informational responses to the server! I've been curious about
this, too but too lazy to look into it - the dishwashing cleansers sold in
"granola" stores (organic, non-toxic to the environment, etc.) are also
citrus-oil based (and at least one has almond oil; not sure if that is
just for the odor).

Tamara

On Thu, 28 Jun 2001, Frank Thomas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers -
}
} This is very interesting, about the orange or citrus-based stuff that is
} good for removing immersion oil. There is also a range of products sold for
} de-greasing bicycle chains and derailleurs, which are based on citrus
} components. I'm no organic chemist myself, but can one of the
} chemistry-oriented people out there tell me why something citrus-based
} should be so good at dispersing oils?
} Call me curious.....and I hope I'm not too off-topic here..
}
} Frank Thomas
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} B3L 4C8
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Thu Jun 28 15:20:49 2001



From: Mike Haugh :      mhaugh-at-resolve3d.com
Date: Thu, 28 Jun 2001 13:15:19 -0700
Subject: Re: sharpening diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Michael Haugh - Director of Operations
Resolution - http://www.resolve3d.com
530 Tamal Plaza, Corte Madera, CA 94925
Office:415/945-7360 x13 FAX:415/927-4495 Cell:415/987-9929
Email: mhaugh-at-resolve3d.com


-----Original Message-----
} From: Ping Li [mailto:pli-at-is.dal.ca]
Sent: Wednesday, June 27, 2001 1:10 PM
To: Microscopy list


Hi, could anyone please give me some information on where I can get my
diamond knives (for semi thin sectioning) sharpened? Any such places in
Canada? I believe the knives were made in Germany. Any information is
highly appreciated. Thank you in advance.

Ping



From daemon Thu Jun 28 15:59:44 2001



From: Shea Miller :      millers-at-em.agr.ca
Date: Thu, 28 Jun 2001 16:58:09 -0400
Subject: plant immunofluorescence-thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks so much for all the info on protocols for plant immunofluorescence... we tried a trial run today on some immature soybeans embedded in paraffin, and got some positive results following the general methods of Tobias Baskin. You have all given great advice, and we have lots to work with to refine our immunoassay.

thanks so much
shea



Dr. S. Shea Miller
Agriculture & Agri-food Canada
Eastern Cereal and Oilseed Research Centre
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
Email: millers-at-em.agr.ca



From daemon Thu Jun 28 16:34:39 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 28 Jun 2001 16:28:36 -0500
Subject: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Micronetsters,
I am interested in this thread about removing immersion
oil, though perhaps diverged a bit from the original post. Over the
years, we have often put oil on a slide and then wished to look
without. In my hands, this is never easy. I have taken chloroform,
acetone, and the like to the coverslip, and wiped and wiped, and it
takes truly many wipes to get oil well and truly off. Small beadlets
of oil remain tenaciously stuck to the glass. If someone does have a
simpler way to get oil off a slide, so well off that there is no
residue left behind, I'd love to know about it.

Thanks,
Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Jun 28 17:04:14 2001



From: Mike Haugh :      mhaugh-at-resolve3d.com
Date: Thu, 28 Jun 2001 14:57:07 -0700
Subject: Re: sharpening diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ping,

We use Micro Star Technologies for sharpening diamond knives. They do a
good job and their turnaround time is very good.

Micro Star Technologies
511 FM 3179
Huntsville, TX 77340-2069
Phone 800/533-2509
FAX 409/294-9861
email mistar-at-msn.com

Mike


Michael Haugh - Director of Operations
Resolution - http://www.resolve3d.com
530 Tamal Plaza, Corte Madera, CA 94925
Office:415/945-7360 x13 FAX:415/927-4495 Cell:415/987-9929
Email: mhaugh-at-resolve3d.com


-----Original Message-----
} From: Mike Haugh
Sent: Thursday, June 28, 2001 1:15 PM
To: 'Ping Li'; Microscopy list


Hi, could anyone please give me some information on where I can get my
diamond knives (for semi thin sectioning) sharpened? Any such places in
Canada? I believe the knives were made in Germany. Any information is
highly appreciated. Thank you in advance.

Ping



From daemon Thu Jun 28 17:45:13 2001



From: Stan Gelles :      sgelles-at-cctlabs.com
Date: Thu, 28 Jun 2001 17:41:00 -0500
Subject: Used TEM/EDX system for asbestos analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are in the market for a used TEM/EDX system that will meet NVLAP asbestos
analysis requirements. Any leads?

Stanley H. Gelles
Senior Group Leader
CC Technologies
614-761-1214
FAX 614-761-1633


From daemon Thu Jun 28 18:52:20 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Thu, 28 Jun 2001 17:45:20 -0600 (MDT)
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sparkle! The wonderful purple glass cleaner stuff. Not available in all
parts of the US, even - I was given a stash by a student from the midwest.
I've heard stories of microscope company service people back east who
stockpile the stuff (definitely couldn't find it in New York). I think
the guy who gave it to me said it was available in True Value hardware
stores; I don't know if they make it.

No financial attachments to Sparkle or True Value, unfortunately.

Tamara

On Thu, 28 Jun 2001, Tobias Baskin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Micronetsters,
} I am interested in this thread about removing immersion
} oil, though perhaps diverged a bit from the original post. Over the
} years, we have often put oil on a slide and then wished to look
} without. In my hands, this is never easy. I have taken chloroform,
} acetone, and the like to the coverslip, and wiped and wiped, and it
} takes truly many wipes to get oil well and truly off. Small beadlets
} of oil remain tenaciously stuck to the glass. If someone does have a
} simpler way to get oil off a slide, so well off that there is no
} residue left behind, I'd love to know about it.
}
} Thanks,
} Tobias
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109 Tucker Hall
} / / / / \ \ \ Biological Sciences
} /_ / __ /__ \ \ \__ University of Missouri
} / / / \ \ \ Columbia, MO USA
} / / / \ \ \ 65211-7400
} / / ___ / \ \__/ \ ____ voice: 573-882-0173
} fax: 573-882-0123
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Fri Jun 29 04:01:00 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Fri, 29 Jun 2001 10:52:55 +0200
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

My guess about limonene is that it is non-toxic, biodegradeable, easily
detectable by (pleasant) smell, a 'waste' product of the food industry,
doesn't need to be mined (environmentally friendly) etc ......

I have seen the bike chain cleaners too and my first reaction was to think
Histoclear!

Again I used a google search

http://www.google.com

so don't take my opinion - try

http://www.floridachemical.com/Default.htm

and the click on 'what is limonene?'

For some more complex chemistry try

http://umbbd.ahc.umn.edu/lim/lim_map.html

http://physics.weber.edu/carroll/Wonder/limonene.htm

I am not associated with any of these concerns.

Have a good summer - I'm off to Finland camping on Sunday - Yippeeeeeeee!


At 13:41 2001-06-28 -0300, Frank Thomas wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sverige

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Fri Jun 29 04:21:25 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Fri, 29 Jun 2001 09:34:41 +1000
Subject: help with Outlook 2000 (Fax setting)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I know that here is some very good experts on computers.. And I cannot
configuration how to do it..

I would like to know how to change setting of the fax in Outlook 2000 from
email only to send and receive faxes..

Ricardo



From daemon Fri Jun 29 04:54:15 2001



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 29 Jun 2001 02:45:25 -0700 (PDT)
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Thu, 28 Jun 2001, Tobias Baskin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Micronetsters,
} I am interested in this thread about removing immersion
} oil, though perhaps diverged a bit from the original post. Over the
} years, we have often put oil on a slide and then wished to look
} without. In my hands, this is never easy. I have taken chloroform,
} acetone, and the like to the coverslip, and wiped and wiped, and it
} takes truly many wipes to get oil well and truly off. Small beadlets
} of oil remain tenaciously stuck to the glass. If someone does have a
} simpler way to get oil off a slide, so well off that there is no
} residue left behind, I'd love to know about it.
}
} Thanks,
} Tobias
}

I have try solvents from alcohol to ammonia and thanks to the advice of
the local confocal Leica rep. Frank Lie, I am now using Windex with great
success for removing immersion oil from oil objectives and COVERSLIPPED
microscope slides.

As for using Histoclear, which I have: it is rather strong smelling and I
am uncertain whether it will destain uncoverslipped stained histo. preps.
Cheers,
Ken

--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203





From daemon Fri Jun 29 08:28:05 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 29 Jun 2001 09:19:29 -0400
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 4:28 PM -0500 6/28/01, Tobias Baskin wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Micronetsters,
} I am interested in this thread about removing immersion
} oil, though perhaps diverged a bit from the original post. Over the
} years, we have often put oil on a slide and then wished to look
} without. In my hands, this is never easy. I have taken chloroform,
} acetone, and the like to the coverslip, and wiped and wiped, and it
} takes truly many wipes to get oil well and truly off. Small beadlets
} of oil remain tenaciously stuck to the glass. If someone does have a
} simpler way to get oil off a slide, so well off that there is no
} residue left behind, I'd love to know about it.
}
} Thanks,
} Tobias
} --
*********************

For years, we have used a 1:1 dilution of Windex & water. Use the
original formula (blue) Windex. this was recommended to use by our
Zeiss representatives and works well on slides and lenses.

lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Jun 29 08:28:05 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 29 Jun 2001 09:16:30 -0400
Subject: RE: sharpening diamond knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi, could anyone please give me some information on where I can get my
} diamond knives (for semi thin sectioning) sharpened? Any such places in
} Canada? I believe the knives were made in Germany. Any information is
} highly appreciated. Thank you in advance.
}
} Ping
*****************

I believe that almost all of the companies that manufacture & sell
diamond knives (Diatome, DDK, Mictostar) will resharpen knives of all
makes. All are the US or have US offices.

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Jun 29 08:42:09 2001



From: David Burton :      dburton-at-nwlink.com
Date: Fri, 29 Jun 2001 06:40:27 -0700
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sparkle glass cleaner is good for cleaning the larger glass surfaces of
the microscope. I don't think it would really work well to remove immersion
oil, we use acetone for that. Acetone is a low health hazard (fingernail
polish remover) and works well for cleaning objectives and eyepieces. Like
any cleaner, even Sparkle, you would never soak an optical element. Apply
the cleaner to something soft and absorbent and use that to clean the
surface. Kimwipes and wooden shafted "Q-tips" work the best.
It's true that Sparkle isn't available everywhere. You can order it from
their website:
http://www.glasscleaner.com/

Dave Burton
Optical Specialist
University of Washington
Scientific Instruments




From daemon Fri Jun 29 09:00:24 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 29 Jun 2001 08:55:23 -0500
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

Just wondering: isn't acetone likely to attack the cements holding the lens
elements in place? Or even some plastic components? Apparently you haven't
had any problems, but I wonder if anyone else has?

Thanks,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: David Burton [mailto:dburton-at-nwlink.com]
Sent: Friday, June 29, 2001 8:40 AM
To: Microscopy-at-sparc5.microscopy.com


Sparkle glass cleaner is good for cleaning the larger glass surfaces of
the microscope. I don't think it would really work well to remove immersion
oil, we use acetone for that. Acetone is a low health hazard (fingernail
polish remover) and works well for cleaning objectives and eyepieces. Like
any cleaner, even Sparkle, you would never soak an optical element. Apply
the cleaner to something soft and absorbent and use that to clean the
surface. Kimwipes and wooden shafted "Q-tips" work the best.
It's true that Sparkle isn't available everywhere. You can order it from
their website:
http://www.glasscleaner.com/

Dave Burton
Optical Specialist
University of Washington
Scientific Instruments




From daemon Fri Jun 29 09:07:43 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 29 Jun 2001 08:59:44 -0500
Subject: Re: help with Outlook 2000 (Fax setting)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fax support is not built into Outlook 2000 and the newer version of Windows
as it was in the older versions. MS does describe the setup on their
knowledge base. Basdically, it is still available by going back and
installing some add-ons that are not part of the regular Windows or Outlook
installation. They are in one of those extra folders that you find on the
CDs. I will send you details directly.

At 09:34 AM 6/29/2001 +1000, you wrote:

} Dear colleagues,
}
} I know that here is some very good experts on computers.. And I cannot
} configuration how to do it..
}
} I would like to know how to change setting of the fax in Outlook 2000 from
} email only to send and receive faxes..
}
} Ricardo

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Jun 29 09:21:52 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 29 Jun 2001 16:19:51 +0200
Subject: Conference: Trends in Nanotechnology 2001 - Reduced Rate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Just a reminder that the hotels are filling up, & the registration rate will
be going up next week for TNT 2001 (Segovia, Spain, September 3-7th). There
are currently some 250 participants from all over the globe, from the fields
of physics, microscopy, FIB, biotech, materials and the semiconductor
industry, as well as dedicated Nanotechnologists and a smattering of venture
capitalists.

More information is at www.cmp-cientifica.com/tnt2001

Regards

Tim

*****************************************************************
Tim E. Harper CEO
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/




From daemon Fri Jun 29 09:33:18 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Fri, 29 Jun 2001 10:26:45 -0400
Subject: Printers and Paper summary (longish)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

Barbara Foster had requested a summary of the replies I had received on this topic. Here it is, FYI.

Paula.

¯----------------------------------------------------------------------------------------------------------------------

1) Avery 9x12" Self - Adhesive Laminating sheets, 50/ pack. #461253 / 73601
The result is a nice glossy finish, and an image that doesn't fade.

2) archival printer ink.
Check the Epson web site for more info about archival inks and printers like the Epson StylusPhoto 2000P.
Wilheim research does the most testing for
archivalness of photographic media. They may have some pointers on the
best ink-paper combos for light stability. http://www.wilheim-research.com

3) enclosing a print in a polyethylene sheet protector (archival grade)

4) printer specific paper
-Stay with the papers recommended for use with the new Epson inks

- try Epson's Heavyweight Matte paper It's cheap ($9/50 sheets
from a discount sppplier), delivers the Epson long-life guarantee, & it has
a robust surface right out of the printer. And beautiful prints.

-There were recently (last 6-8 months) some issues with color shift
on some Epson papers. Epson determined the shift was from high
concentrations of ozone, and not due to exposure to light. Epson
recommends Heavyweight Matte or Epson Photo paper for maximum
lightfastness.

-if the "Photo Quality Glossy Paper" isn't new, it may be the paper's fault - it's been reformulated to deal with this problem.
See http://www.luminous-landscape.com/1280.htm &
http://www.wilhelm-research.com/


5) printer settings
- make sure that when you print B&W prints, set the printer to "B&W". Colors can appear when "color" is selected, and the color printing mimics
B&W

6) protective sprays
- Luminos http://www.lumijet.com has a spray called
"Imagesheild" to protect prints. $18/ can.
Quoting their website:
"LUMIJET IMAGESHIELD will become your favorite print protector.
It*s a convenient ozone-friendly low-odor aerosol spray that significantly
improves wet-fastness. In addition to protecting delicate inkjet images
from moisture, it also shields images from fingerprints and harmful UV rays.
This unique product will not only help extend useful print life,
it is totally transparent; preserving the original print surface
characteristics. From dead matte to glossy, or from canvas to silver foil,
you*ll never know it*s there."

-Krylon clear acrylic spray
Use many light coats. If you really wet the surface, the paper can become
translucent.

-Johnson's Paste Wax.
It works on some inks and papers, not all. It does offer some protection against UV. It does protect against moisture, finger prints, dust and scratches. It does change the finish.
If the wax causes the ink to smear a fixative spray will usually stop that.
Test it for several weeks before putting it on an expensive display.

Comments:
Do not use the printer for archival printing. For long lasting prints use a
silver halide digital printer (or dye sub) or traditional dark room processing.

Ink-jet prints have never been designed for long term preservation.
the inks used in those printers are made of dyes, which are very
sensitive to U.V electromagnetic waves present in fluorescent lamps and sun rays. Dyes are not chemically fixed to the fibers, so can cause the smudging problems. The use of a fixative to stabilize the colour is only a temporary and partial solution: the polymers spayed on the surface of the print might partly protect the dyes by filtering some U.V rays but doing so, will tend to yellow with time and give a yellowish cast to your images.
Digitizing images is not a complete longterm solution either. For best preservation of images, save many copies on CDs, and check them often for deteroiration and update the formats as needed to be sure they are always accessible (tiff uncompressed files) and keep it in a cool place, with low humidity (30% RH), or rent a place in a specialised data keeping system firm.

¯----------------------------------------------------------------------------------------------------------------------


Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca


From daemon Fri Jun 29 09:34:45 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 10:30:03 -0400
Subject: RE: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You are absolutely right, and most of the time we behave just as you
describe us. Patient, etc. One purpose of my tirade was to point out that
there are differences between today and yesterday that give ALL of us
choices to make. I know a high school student who failed the year, because
he wanted to punish his parents. When he told me what he had done, I
responded that I knew a young man just like him when I was in high school.
His solution was to get straight A's so he could become more quickly
independent.
Science is our own, very human, endeavor. Those of us who do
science must constantly work to remove the temptations to embellish our
results, to twist the statistics and to 'jump' to conclusions. But, and
here's where I differ perhaps slightly. Sometimes, there is a good reason
to yell! My wife hates my streak of sarcasm! But I was raised on the
stuff. When I did something 'dumb,' everyone who loved me, joined in the
derisive repair. I learned to think at my dinner table before I was 10. I
did not like to be the but of everyone's attention. My Mom taught, then I
taught. I only stopped, because no one [too few] wanted to learn any more
[bad generalization]! Go to college today, and what do you find? Young
people unprepared for the rigor. Young people unprepared for the
intellectual dance - as we used to call it. Young people who don't know
what they want to do and are unwilling to work to find out. "They only do
what they are taught!" This is my mantra when I look for explanations in
the mirror. My older son had to learn - for the test - to name NH3 as
"ammonium", because his chemistry teacher would not believe the Handbook of
Chemistry and Physics. My chemistry teacher in public high school took
chemistry courses at Rutgers every third summer. He was the expert in
chemistry. My physics teacher had a PhD in the subject. My biology teacher
went on to be the chair of Physiology at a large Southern medical school.
If I wanted to complain about a professor at Lehigh, the Dean would show me
a list of nearby schools that had openings. The attitude was clearly
communicated. The greatest lessons in life are those that teach one to
manage the worst parts of it. If I failed a course, it was MY failure - and
it was at least MY problem! I wanted to be what I am, once I figured
everything out. The only advice I ever received, when I was having problems
with learning, was, "Study harder." My Mom just kept on telling me to keep
on trying as hard as I could. If I were young now, I'd be in group therapy
and zapped on some pharmaceutical, if the system had its way.
Given my experience, I hope it is not difficult to understand why I
might not appear to understand the problems of others. BUT! If I see a
young person in science who is poorly informed about how it is done, then I
will act in that person's behalf even if I am thought, and reported, to be
insensitive.
When I was a graduate student, we were all ordered to write
faculty/course evaluations. I did. I wrote analyses of each course, and
every one of them was critically positive. Not one word of my analyses were
printed. If I had demonized one of my professors, I would have been writing
novels today. The people who ordered the writings were ex-faculty
administrators who were pandering to the new consumer mentality in
education. If I had gone to my undergraduate department chair to complain
about a grade, he would have told me that I was wasting his time. Five
years later, there were lines of boomers outside of department offices. The
generation of manipulators had arrived! While they were standing in line, I
was learning. While I was learning, they were just getting warmed up!
I have had ideas snitched! Sometimes I even gave them away. On the
other hand, I have also had the other side of the experience. For six
months of learning followed by 30 minutes of consultation and work, I had
the good fortune to become a molecular biologist. For that effort, the
individual to whom I gave my best remembered my part - even after he had
spent years doing the work - and included me in the author list for a
gene(TERE1) that mapped to Chromosome 1 and appeared in print in a Nature
publication. He did NOT have to do that. There was NO obligation nor
agreement. I helped because he asked. People are basically good,
especially scientists, and I always start with TRUST, and I always will.
You are still absolutely correct.

Regards for YOUR sensitivity,

Fred Monson

} ----------
} From: Sousan Abolhassani
} Sent: Thursday, June 28, 2001 8:07 AM
} To: Paul Webster
} Cc: MSA listserver submission
} Subject: Re: long-term storage OR what has happened?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Paul,
}
} The point about this server is that it is used by
} a very heterogeneous public from which a non-exclusive
} list is the following:
}
} students,
} professors,
} scientists/
} researchers
} technical staffs
} suppliers
} producers
} specialists
} non-specialists
} etc.
} etc.
} etc.
}
} and all these from so many different backgrounds with so
} many different problems and even from so many different countries...
} That if we want to teach all of them to behave as we expect
} before they post a query, the server could go to early retirement.
}
} I guess the philosophical attitude that one should have is to
} really try and understand both sides of the discussion.
}
} 1- on one hand the trust that professor Monson is demanding
} is not there because our users are a small sample of our
} society. How many times have we heard that people have had
} their ideas picked by others and their publications stolen
} by others and .... in the very scientific community that most
} of us hoped to be the purest part of the society. Still
} the scientists are generally speaking very sincere people.
} So if someone is not having trust to others, it's the
} consequence of what they have observed in their environment.
}
} 2- I do not know if Rachel is a student (may be not), and if she
} really was not trusting or just did not think to write the reason and
} so on, so we do not discuss the case on a specific way.
} But talking about going to the library, there are labs
} where the students have really not access to the books and there
} are places where superiors do not give the support the students
} and other researchers' need, or simply there are not
} enough books available.
}
} So there are always reasons for the way people behave
} and the more our society goes towards a very materialistic and
} "win whatever the situation" attitude, the more the arguments
} of professor Manson will be applicable because the more people
} will behave in the way he is not happy about, and I understand
} and apreciate his point.
}
} All that apart, it is better to listen to people,as often things
} that are not to be done are not published and are only "known".
} Of course provided that answers are given generously, as arrogance
} for giving is so destructive, even for donator.
} I therefore agree with the fact that we should just be able to
} ask the question and someone will react to give us an answer.
}
} I think that professor Monson has evoked a point that is of
} great importance and that is the human aspect of all we do, as
} "human" is what we are hoping to be at a first place.
} That needs sensitivity... and continuous effort.
}
} Regards,
}
} Sousan
}
}
} Paul Webster wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Dear Colleagues,
} }
} } The messages from Professor Monson in reply to a question about
} long-term
} } tissue storage makes me wonder what has happened to this listserver?
} }
} } His message was highly entertaining and even if the poster of the
} original
} } message may have felt "picked on", there was no reason to expect him to
} post
} } an apology, no matter how informative it was.
} }
} } Far too many posters use this listserver as a way of finding a quick fix
} to
} } simple solutions to technical problems that can be easily researched in
} text
} } books.
} }
} } Often these messages contain little background information on the
} technical
} } problem under investigation. Even so, many of these questions get full
} } attention and detailed replies, even if the replies are not posted on
} the
} } main list for public view.
} }
} } What has happened to the free and open discussion of issues and
} protocols
} } that we used to have? Are people so afraid of being accused of being
} } incorrect that they do not post their replies openly? Or maybe people
} are
} } afraid of being accused of being impolite.
} }
} } I look forward to controversy and hope that my comments are read
} carefully
} } and replied to with vigor. Only in this way will I be able to learn new
} } thing, and this is the main reason I still subscribe to this listserver.
} }
} } If I am factually wrong about something, tell me. It is better to find
} this
} } out on the listserver than in a more professional (or cruel)
} environment.
} }
} } As for discussion subjects: why was there no comment about the
} unadvisable
} } suggestion made about storing samples in a frozen state? Would anyone
} } working with EM really choose to store their samples at -20 degrees
} before
} } processing for TEM?
} }
} } Where is all the discussion about extraction that can occur when storing
} } samples in alcohols or buffers, or on the contamination that I know
} grows in
} } everyones old cacodylate buffers?
} }
} } Do we not comment because we know it has all been published in
} textbooks?
} }
} } Dear Profesor Monson, do not feel bad about improving our general
} knowledge
} } and our professional etiquette. I for one support your views and find
} your
} } comments useful and entertaining. Please keep up your submissions.
} }
} } Best regards,
} }
} } Paul Webster
} }
} } Paul Webster, Ph.D.
} } Scientist II and Director
} } Ahmanson Center for Advanced EM & Imaging
} } House Ear Institute
} } 2100 West 3rd Street
} } Los Angeles
} } CA 90057
} }
} } pwebster-at-hei.org
} } p: 213 273 8026
} } f: 213 413 6739
} } http://www.hei.org/research/depts/aemi/aemi.htm
}
}


From daemon Fri Jun 29 09:36:16 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 10:31:53 -0400
Subject: RE: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gareth,

You are teaching again!

Respectfully,

Fred Monson

} ----------
} From: Gareth Morgan
} Sent: Thursday, June 28, 2001 8:18 AM
} To: Paul Webster; MSA listserver submission
} Subject: Re: long-term storage OR what has happened?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Colleagues
}
} I agree wholeheartedly with Paul in his comments regarding the mails from
} Frederick Monson - no need for apology, interesting and informative
} replies.
}
} Higher education seems to be locked in an apparently paradoxical situation
} with students being expected to 'take responsibility for their own
} learning', the use of Student Centred & Problem Based Learning etc. The
} paradoxical part is that they do not have the appropriate intellectual
} tools to be able to carry out such assignments. Frustration with their lot
} then spills out over tutors who are expected to take responsibility when
} things go wrong (but mostly no feedback when things work).
}
} Ask me a question and I will give you the most appropriate answer I can.
} The answer may be wrong of course, possibly because I have an incorrect
} understanding of the concepts involved, possibly because I was asked the
} wrong question. If I respond to a question with a question of my own it is
} because I am trying to seek clarification of what EXACTLY is being asked.
} That, as I see it, is how we work in science.
}
} Paul says - "If I am factually wrong about something, tell me. It is
} better
} to find this out on the listserver than in a more professional (or cruel)
} environment."
}
} I agree absolutely here too - it is easier to fight tooth and claw here
} and
} come to some agreement or compromise than if we are addressing 500 people
} at a conference and one of them asks a simple question to which we have no
} answer.
}
} My appeal to those posing questions is to give as much background
} information as possible and to respond clearly to questions on
} clarification.
}
} My appeal to those answering is to identify clearly what is hearsay "in my
} experience" "I got that straight from a guy in the pub last night".
} Identify also the context of the answer/solution to the question/problem
} and refer to published data whenever possible.
}
} I look forward to a lively, ongoing debate (even though I am going for my
} summer break tomorrow!).
}
}
}
}
} Med v?nliga h?lsningar/With best wishes
}
} Gareth
}
} "Close your eyes and look. What you saw at first is there no more; and
} what
} you will see next has not yet come to life" Leonardo da Vinci (1452-1519).
}
} http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se
}
} Tel +46 8 728 3734
} Fax +46 8 728 3688
}
} Gareth Morgan MPhil MSc FIBMS,
} Institutionen f^r Mikrobiologi, Patologi och Immunologi(IMPI), H5,
} Karolinska Institutet,
} Avdelningar f^r biomedicinsk
} laboratorievetenskap och
} biomedicinska ?mnen,
} Lindhagensgatan 92, Box 12773,
} S 112 96, Stockholm
} Sverige
}
} OBS! Bes^ksadress: Lindhagensgatan 92
} NB! Visiting address:Lindhagensgatan 92
}
}


From daemon Fri Jun 29 09:37:26 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 10:33:04 -0400
Subject: RE: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Didn't that 'stuff' still have Xylene in it?

Fred Monson

} ----------
} From: Gareth Morgan
} Sent: Thursday, June 28, 2001 7:45 AM
} To: Christine Edly; Microscopy-at-sparc5.microscopy.com
} Subject: Re: immersion oil remover?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} I have used the 'orangey' smelling solvent under the brandname of
} Histoclear. It is supposedly extracted large scale from citrus peel and is
} the composed of the 'zest' that comes out as a spray when we peel oranges.
}
} I couldn't comment on its use for removing immersion oil as you describe.
} I
} have only used it as a xylene substitute for tissue processing tissues and
} also for clearing stained sections prior to mounting.
}
} It is said to be safer than xylene but some of the people I worked with at
} the time complained of headaches so we stopped using it despite never
} proving that it was the cause of the headaches.
}
} Hope this helps!
}
}
}
} At 17:42 2001-06-27 -0500, Christine Edly wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Greetings!
} }
} } I'm in search of something that will remove immersion oil from slides of
} }
} } blood smears (no coverslips) without damaging the specimens. A friend
} } told that such a chemical exists, but could not remember the name. It
} } supposedly smells like oranges, and xylene was once used for the same
} } purpose. If you have any ideas as to the name of this mystery chemical,
} }
} } I'd sure appreciate it.
} }
} } Regards,
} } Christine Edly
} } University of Oklahoma
} }
}
}
} Med v?nliga h?lsningar/With best wishes
}
} Gareth
}
} "Close your eyes and look. What you saw at first is there no more; and
} what
} you will see next has not yet come to life" Leonardo da Vinci (1452-1519).
}
} http://www.ki.se/biomedlab e-mail Gareth.Morgan-at-impi.ki.se
}
} Tel +46 8 728 3734
} Fax +46 8 728 3688
}
} Gareth Morgan MPhil MSc FIBMS,
} Institutionen f^r Mikrobiologi, Patologi och Immunologi(IMPI), H5,
} Karolinska Institutet,
} Avdelningar f^r biomedicinsk
} laboratorievetenskap och
} biomedicinska ?mnen,
} Lindhagensgatan 92, Box 12773,
} S 112 96, Stockholm
} Sverige
}
} OBS! Bes^ksadress: Lindhagensgatan 92
} NB! Visiting address:Lindhagensgatan 92
}
}


From daemon Fri Jun 29 09:52:38 2001



From: JHoffpa464-at-aol.com
Date: Fri, 29 Jun 2001 10:47:55 EDT
Subject: renal if storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi all,
we are doing IF on renals for diagnostic work. does anyone know how long we
are required to store them?
john hoffpauir
cooper hospital


From daemon Fri Jun 29 10:07:20 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 10:59:47 -0400
Subject: RE: plant immunofluorescence-thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I bet we would all like to see some results when you get them.

Regards,

Fred Monson

} ----------
} From: Shea Miller
} Sent: Thursday, June 28, 2001 4:58 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: plant immunofluorescence-thanks
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks so much for all the info on protocols for plant
} immunofluorescence... we tried a trial run today on some immature soybeans
} embedded in paraffin, and got some positive results following the general
} methods of Tobias Baskin. You have all given great advice, and we have
} lots to work with to refine our immunoassay.
}
} thanks so much
} shea
}
}
}
} Dr. S. Shea Miller
} Agriculture & Agri-food Canada
} Eastern Cereal and Oilseed Research Centre
} Central Experimental Farm
} Ottawa, Ontario
} Canada K1A 0C6
} Phone: (613) 759-1760
} Fax: (613) 759-1701
} Email: millers-at-em.agr.ca
}
}
}


From daemon Fri Jun 29 10:34:00 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 11:26:13 -0400
Subject: RE: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Let's See Now,

The classic blood stains are the dried salts of cationic(methylene
blue) and anionic(eosin) dyes dissolved in methanol. So, acid will remove
one and base will remove the other, and the aromatic organic is what we no
longer want to use. How about a non-ionic detergent? Is that what's in the
"Orange" mystery?

Well here's my offering for something 'orangy'. I acquired it a
Home Depot. "Commercial ZEP Citrus All Purpose Cleaner". The Directions
say that you can use it full strength or diluted. Probably doesn't have any
of THOSE aromatics. Nothing on the label. Looks like a typical proprietary
scientific mixture. Just like "Histoclear" and "Paraplast." [Why does that
period look so out of place?] "Removes grease, oil and soils [Sic!] from
concrete, metal and other hard surfaces."

Glass is hard unless one has a very long perspective. I wonder. If
I had some old slides, I could try it. Full strength or a dilution series?
Ten-fold or two-fold? Water or alcohol as a vehicle? Wonder whether
"triphosphate" would work. Did a fine job on the driveway. Time for lunch!
I'll stop by Home Depot on the way back.

Respectfully submitted in partial fulfillment of requirements for.......,

Fred Monson [Is he this way all the time? Only when awake.]
} ----------
} From: Christine Edly
} Sent: Wednesday, June 27, 2001 6:42 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: immersion oil remover?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} I'm in search of something that will remove immersion oil from slides of
}
} blood smears (no coverslips) without damaging the specimens. A friend
} told that such a chemical exists, but could not remember the name. It
} supposedly smells like oranges, and xylene was once used for the same
} purpose. If you have any ideas as to the name of this mystery chemical,
}
} I'd sure appreciate it.
}
} Regards,
} Christine Edly
} University of Oklahoma
}
}


From daemon Fri Jun 29 10:39:49 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Fri, 29 Jun 2001 08:28:50 -0700
Subject: RE: TEM-Ultrathin Film-Thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear Colleagues,

I am very grateful to everybody for your help with regards to
finding suitable TEM grid film for small particles. The number of responses
were really overwhelming. Special thanks to those who were willing to send
me grids to try out. Thank you all once again.

Regards,

Thearith Ung



From daemon Fri Jun 29 10:44:38 2001



From: Hong Yi :      hyi-at-emory.edu
Date: Fri, 29 Jun 2001 12:04:04 -0400
Subject: Preservation and staining of bacterial capsules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, everyone. I am posting this for someone else. However replies can be
either posted on the server or sent to my email address directly. I will
forward all to her. Thank you in advance.

Hong


Hi Hong,

Okay... here is a paragraph or so about what we are trying to
see that you can paste onto the list serv.


We are attempting to visualize the polysaccharide capsule of
Neisseria meningitidis serogroup B by electron microscopy. The
capsule is (alpha2-8)-linked polysialic acid with about 200-300
residues per polymer. We have tried staining the negatively
charged capsule with Ruthenium red, followed by embedding,
sectioning, and counterstaining. However, it appears that
only the inner and outer membranes were stained and not the
capsule (we did capsule + and capsule - controls). In a previous
paper that effectively stained capsule of E. coli K5, the cells
were washed with capsule-specific antibody before fixation to
stabilize the capsule. We have not attempted the procedure with
the antibody stabilization yet. Please let me know if this would
be recommended or if there are any other suggestions. Thank you.


Christy



From daemon Fri Jun 29 10:52:31 2001



From: Shea Miller :      millers-at-em.agr.ca
Date: Fri, 29 Jun 2001 11:51:15 -0400
Subject: counterstaining for immunofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again;
we are having a wee bit of trouble distinguishing our signal from the autofluorescence in our immunoassay on immature soybean seeds/seed coats. So far, we have used a light stain of toluidine blue, but I'm not completely happy, and am considering Evans Blue instead. What do others use to quench the yellow autofluorescence (our Ab is FITC labelled) in plant tissues, and when do you actually apply the counterstain? Before the Ab incubations, or after??

thanks so much again in advance
shea



Dr. S. Shea Miller
Agriculture & Agri-food Canada
Eastern Cereal and Oilseed Research Centre
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
Email: millers-at-em.agr.ca



From daemon Fri Jun 29 11:05:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 11:58:44 -0400
Subject: RE: Today's Real Humor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I never heard that before. After 36 years of marriage, and I find this out
NOW?

Fred Monson
} ----------
} From: Nicol Aitken
} Sent: Friday, June 29, 2001 10:43 AM
} To: 'Monson, Frederick C.'
} Subject: RE: Today's Real Humor
}
} I'll answer e, even though strictly speaking osculation need not occur!
} Nick
}
} -----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
} Sent: Thursday, June 28, 2001 1:30 PM
} To: 'Microscopy Listserver'
} Subject: Today's Real Humor
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Question from an old exam given to students who demanded MORE MULTIPLE
} CHOICE!
}
} Which of the following is NOT involved with human reproduction?
}
} a. transcription
} b. replication
} c. translation
} d. osculation
} e. none of the above
}
} Answer tomorrow.
}
}
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}


From daemon Fri Jun 29 11:05:02 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 29 Jun 2001 12:01:20 -0400
Subject: Re: immersion oil remover?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps I'm missing something from the biological side with which I am
not familiar. However, for my purposes, it has always been very easy to
remove immersion oil using a non-polar organic such as one of the
following: hexane, iso-octane, VM&P Naphtha (Varnish Maker's and
Printer's Naphtha from the hardware store), "petroleum ether" (not
really a C-O-C ether but a mixed alkane distillate), ligroine, etc.


These have the following attributes - they are extremely low in
viscosity so they penetrate any porosity quickly and dissolve the oil;
they evaporate quickly so that any residual oil quickly becomes
apparent, they do not swell or dissolve most mounting materials
(limonene - the environmentally friendly base of many "green" solvents,
is a potent stripper of polymers); and their high vapor pressure makes
them easy to eliminate prior to SEM examination. I have routinely used
immersion oil to temporarily coverslip geological thin sections for
optical microscopy prior to microprobe work and then removed the oil
with these solvents. Several drops in succession, less than a
milliliter total, is sufficient to clean one slide.

John Twilley


Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Please post informational responses to the server! I've been curious about
} this, too but too lazy to look into it - the dishwashing cleansers sold in
} "granola" stores (organic, non-toxic to the environment, etc.) are also
} citrus-oil based (and at least one has almond oil; not sure if that is
} just for the odor).
}
} Tamara




From daemon Fri Jun 29 11:10:58 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 12:05:31 -0400
Subject: RE: Today's Real Humor Answer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As we have been instructed for the past twenty years, nothing is NOT
involved with that stuff.

So, 'e' is the correct answer. My students learned to hate multiple choice
as much as I did.

Regards to all, with advice to my son. Always brush your teeth first, so
the kisses are sweet. And he asked, "Mint or wintergreen?"

Have a nice weekend and remember, we can all get away with this because
someone once worked on the 4th of July!

Fred Monson


} ----------
} From: Monson, Frederick C.
} Sent: Thursday, June 28, 2001 1:30 PM
} To: 'Microscopy Listserver'
} Subject: Today's Real Humor
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Question from an old exam given to students who demanded MORE MULTIPLE
} CHOICE!
}
} Which of the following is NOT involved with human reproduction?
}
} a. transcription
} b. replication
} c. translation
} d. osculation
} e. none of the above
}
} Answer tomorrow.
}
}
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}


From daemon Fri Jun 29 12:30:21 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 29 Jun 2001 13:23:30 -0400
Subject: Microscope Maintenance Companies - Others?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's an old list, checked and amplified, that came to me from "Microscopy
Today"
I have checked all of these URL's

www.home.earthlink.net/~veilcs/ (EM+ special in Philips) (west Coast - USA)
www.southernmicro.com (Georgia) (Research LM)
www.dscoptical.com/services.htm (Boston?) (LM)
www.caleywhitmore.com (Boston?) (LM)
www.opticalexpertise.com (NY) (connection failure?)
www.mwrn.com/product/microscope/repair.htm (LM and EM)
www.sciscope.com/field-service.htm (30+ states) (up to Advanced LM)
www.svms.com/services/index.html (CA) (LM)
www.meyerinst.com (TX) (up to advanced LM)
www.microresource.com/services2.htm (IL) (LM)
www.allometrics.com/microscope_serv.htm (LA)(LM)
www.labworksinc.com (San Francisco) (Limited to specialized LM scope
accessories)
www.mikronet.com (San Diego) (general LM services)
www.uams.edu/oas/OES/oesilab.htm (AR) (University of Arkansaw LM support
facility)
www.uni.edu/pur/mms/equipment.html(U. N. Iowa) (Wide range of equipment
including EM's)
www.dominionmicroscope.com/services.htm (Richmond, VA) (LM and associated
equipment)
www.eosltd.co.uk/ (UK.COM)
www.emsys.co.uk/ (UK.COM)
www.hii.hitachi.com/svcem.htm (Hitachi)
www.leo-usa.com/ (LEO)
www.feic.com/products/index.htm (FEI - Philips)
www.jeol.com/locations/service/service.html (JEOL Service, USA)


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Fri Jun 29 13:18:19 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 29 Jun 2001 11:12:23 -0700
Subject: Confocal questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Some faculty here are entertaining the idea of developing a confocal core
facility. There is currently a single confocal microscope on campus in an
individual PI's lab and it is not as available to others as they might like
due to that lab's workload, location, etc.

I have been invited to join the group investigating the possibility of a
second, more general access microscope, probably supported by several
departments. I am currently the person responsible for the EM lab on
campus, we have a TEM and an SEM, plus most of the standard prep.
equipment. We also do some digital imaging. The EM business is not booming,
save for a few hardcore users.

Since I am one of the 'Mr. Microscopes' here, I would like to join this
group with a running start. I ask for your sage advice regarding the
potential good and bad of this proposal and any comments you might offer on
the state of confocal and such a lab.

I know a lot about microscopes, less specifically about confocals, but I
could learn. If the microscope was put into the existing EM lab, I have a
separate room, bench space, hoods etc. Just about anything needed to do
microscopy. The only drawback would be that we are in a different building
than most of the users. Its not a bad walk, but how big a deal is it to
prepare specimens away from one's own lab? It would be pretty fair for the
multiple users, since none of them are all in the same building either,
they would all have to do some traveling to get here.

I am pretty sure there will be the usual squabbling that goes along with
central facilities, like who gets to use it if there is competition, who
will pay for it, etc. Is that likely to be any worse then with any shared
equipment? Am I asking for trouble to have it added to the EM Lab?

Do you have an experience with the kind of day to day issues that a lab
like this might generate. Are there lots of supplies that need to be
ordered and kept on hand? Is the equipment reliable and are there any
special features of one particular type of instrument that would make it
more appropriate for such a general use facility vs. something used by an
individual lab?

Your comments will be carefully considered and as always, greatly appreciated.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Fri Jun 29 14:38:20 2001



From: Kevin W. Eliceiri :      eliceiri-at-facstaff.wisc.edu
Date: Fri, 29 Jun 2001 14:31:56 -0500
Subject: Re: removing immersion oil, not histoclear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

best,
kevin


From daemon Fri Jun 29 16:17:34 2001



From: Gordon Couger :      gordon-at-couger.com
Date: Fri, 29 Jun 2001 16:07:07 -0500
Subject: Re: long-term storage OR what has happened?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The department I worked for took a different approach to the students not
"ready for the dance". Every Thursday night the lab was open a faculty
member from assistant professor to department head was there from 7:00 pm
until at least 11:00 pm to help them with what ever problems they had not
just Ag Engineering courses. I worked a research computer programmer and we
didn't teach any programming courses and I had not teaching duties at all
but any student could come to me any time with a computer problem and the
same was true for any member of the staff or faculty for any problem the
students had.

No grad students taught courses or labs.

The policy paid off real well. I don't remember when one of our students
didn't get a job after
graduation and many have gone on to very good things. This years senior
design class is applying for patent on part of their class project.

I realize that farm kids are a good deal better prepared for life than most
students but their
education is usually a good deal poorer in science and math than the kids
from the city and they have a good deal of catching up to do.

You are short changing your students if you just present the knowledge and
walk away.

I realize that the approach we used is difficult to use in departments with
large number of students. Turning education into a mass market commodity is
IMO much of what is wrong with it.

Gordon

Gordon Couger


Stillwater, OK


----- Original Message -----
} From: "Monson, Frederick C." {fmonson-at-wcupa.edu}
}
}
} You are absolutely right, and most of the time we behave just as you
} describe us. Patient, etc. One purpose of my tirade was to point out
that
} there are differences between today and yesterday that give ALL of us
} choices to make. I know a high school student who failed the year,
because
} he wanted to punish his parents. When he told me what he had done, I
} responded that I knew a young man just like him when I was in high school.
} His solution was to get straight A's so he could become more quickly
} independent.
} Science is our own, very human, endeavor. Those of us who do
} science must constantly work to remove the temptations to embellish our
} results, to twist the statistics and to 'jump' to conclusions. But, and
} here's where I differ perhaps slightly. Sometimes, there is a good reason
} to yell! My wife hates my streak of sarcasm! But I was raised on the
} stuff. When I did something 'dumb,' everyone who loved me, joined in the
} derisive repair. I learned to think at my dinner table before I was 10.
I
} did not like to be the but of everyone's attention. My Mom taught, then I
} taught. I only stopped, because no one [too few] wanted to learn any more
} [bad generalization]! Go to college today, and what do you find? Young
} people unprepared for the rigor. Young people unprepared for the
} intellectual dance - as we used to call it. Young people who don't know
} what they want to do and are unwilling to work to find out. "They only do
} what they are taught!" This is my mantra when I look for explanations in
} the mirror. My older son had to learn - for the test - to name NH3 as
} "ammonium", because his chemistry teacher would not believe the Handbook
of
} Chemistry and Physics. My chemistry teacher in public high school took
} chemistry courses at Rutgers every third summer. He was the expert in
} chemistry. My physics teacher had a PhD in the subject. My biology
teacher
} went on to be the chair of Physiology at a large Southern medical school.
} If I wanted to complain about a professor at Lehigh, the Dean would show
me
} a list of nearby schools that had openings. The attitude was clearly
} communicated. The greatest lessons in life are those that teach one to
} manage the worst parts of it. If I failed a course, it was MY failure -
and
} it was at least MY problem! I wanted to be what I am, once I figured
} everything out. The only advice I ever received, when I was having
problems
} with learning, was, "Study harder." My Mom just kept on telling me to
keep
} on trying as hard as I could. If I were young now, I'd be in group
therapy
} and zapped on some pharmaceutical, if the system had its way.
} Given my experience, I hope it is not difficult to understand why I
} might not appear to understand the problems of others. BUT! If I see a
} young person in science who is poorly informed about how it is done, then
I
} will act in that person's behalf even if I am thought, and reported, to be
} insensitive.
} When I was a graduate student, we were all ordered to write
} faculty/course evaluations. I did. I wrote analyses of each course, and
} every one of them was critically positive. Not one word of my analyses
were
} printed. If I had demonized one of my professors, I would have been
writing
} novels today. The people who ordered the writings were ex-faculty
} administrators who were pandering to the new consumer mentality in
} education. If I had gone to my undergraduate department chair to complain
} about a grade, he would have told me that I was wasting his time. Five
} years later, there were lines of boomers outside of department offices.
The
} generation of manipulators had arrived! While they were standing in line,
I
} was learning. While I was learning, they were just getting warmed up!
} I have had ideas snitched! Sometimes I even gave them away. On the
} other hand, I have also had the other side of the experience. For six
} months of learning followed by 30 minutes of consultation and work, I had
} the good fortune to become a molecular biologist. For that effort, the
} individual to whom I gave my best remembered my part - even after he had
} spent years doing the work - and included me in the author list for a
} gene(TERE1) that mapped to Chromosome 1 and appeared in print in a Nature
} publication. He did NOT have to do that. There was NO obligation nor
} agreement. I helped because he asked. People are basically good,
} especially scientists, and I always start with TRUST, and I always will.
} You are still absolutely correct.
}
} Regards for YOUR sensitivity,
}
} Fred Monson




From daemon Fri Jun 29 16:19:55 2001



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 29 Jun 2001 16:16:12 -0500
Subject: Microscope magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Need some help trying to figure the magnification of some photos taken with
the following:

Nikon Optiphot-Pol Microscope
CF eyepiece 10X
CF objective 20X

with Nikon AFX-II camera control assembly using a Polaroid 4x5 film holder
with a 4X marked on it.

Focus for images was done through the monocular eyepiece on the camera
assembly.

I assume that my magnification though the microscope eyepiece would be 30X
I assume that the magnification to the camera back would be 24X

Sorry if this seems like a silly question but I do not do much optical
microscopy and got to thinking that it may be more than just the sum of the
optics magnification.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Fri Jun 29 16:33:05 2001



From: Paul Webster :      pwebster-at-hei.org
Date: Fri, 29 Jun 2001 14:29:37 -0700
Subject: Technologist position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The House Ear Institute, a Los Angeles-based, non-profit research and
education center dedicated to the hearing-impaired, has experienced
tremendous growth over the past 50 years. We currently have an exceptional
opportunity for a motivated and innovative self-starter.

You will support research projects in the cell and molecular biology of
hearing. You will also perform advanced research procedures, such as
immunoelectron microscopy and microwave processing, and maintain records.
Requires a detail-oriented, flexible and enthusiastic team player with a BS
in the biomedical field, as well as knowledge of TEM and SEM, specimen
preparation for TEM and SEM, darkroom developing/printing, and digital image
capture/manipulation. Must have efficient communication skills. Familiarity
with electron microscopes, mechanical/electronic equipment, vacuum systems,
and Unix, PC or Mac is ideal. Experience in cell culture, microbiology or
protein purification is useful but not essential.

The Ahmanson Advanced Electron Microscopy and Imaging Center was recently
established to perform independent research and provide support to a cell
and molecular biology program in hearing research. The Center has a CM 120
BioTwin TEM and XL30 SFEG SEM. Specimen preparation equipment include
ultramicrotomes for resin and cryosectioning, sputter coaters, vacuum
evaporator, freeze-substitution, freeze-fracture and rapid freezing devices,
a microwave processor, digital and stereologucal imaging capabilities and an
SGI octane with 3-D reconstruction software.

The House Ear Institute, located in the downtown Los Angeles area, is a
non-profit research and education organization dedicated to improving the
lives of people who have hearing and hearing related disorders. The House
Ear Institute is an Equal Opportunity Employer.

For consideration, please submit resume and cover letter to: House Ear
Institute, Human Resources, 2100 W. 3rd St., Los Angeles, CA 90057-1922.
FAX: (213) 483-8789. E-mail: jobs-at-hei.org.

When applying, please state that you saw this position advertised on the MSA
listserver.

Regards,

Paul Webster




Paul Webster, Ph.D.
Scientist II and Director
Ahmanson Center for Advanced EM & Imaging
House Ear Institute
2100 West 3rd Street
Los Angeles
CA 90057

pwebster-at-hei.org
p: 213 273 8026
f: 213 413 6739
http://www.hei.org/research/depts/aemi/aemi.htm






From daemon Fri Jun 29 16:39:04 2001



From: Andreas Taubert :      taubert-at-seas.upenn.edu
Date: Fri, 29 Jun 2001 17:46:33 -0400
Subject: support film thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

this is some kind of follow-up question to Thearith's grid problem. We are looking at about 2 nm
features in a polymer matrix. The features have very low contrast with respect to the all-carbon
matrix due to the fact that they mostly contain O and a little Zn. For sample prep reasons we need
to support the samples. Now: Are SiO and Si nitride coatings thin enough to still see such things ?
The available thicknesses of these films seem very large compared to conventional C films.

TIA,

Andreas

*************************************************
Dr. Andreas Taubert
Dept. of Materials Science and Engineering
3231 Walnut Street
University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************



From daemon Fri Jun 29 17:09:56 2001



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 29 Jun 2001 17:05:48 -0500
Subject: Microscope Magnification correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Not thinking today, in my previous message I made assumptions about total
magnification being the sum of the objectives but should have been the
product.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Fri Jun 29 17:21:00 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Fri, 29 Jun 2001 17:58:09 -0500
Subject: Microscope Maintenance Companies - Others?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, Fred, we published this list in Microscopy Today. We got it from a lady
from Australia, whose name currently escapes me.
Due to the interest in this topic, allow me to request that all readers
contact companies that they know that does microscopy (all
techniques)independent service. Ask them to contact me by email with the
following:
1) Name of organization.
2) Contact info (tel. number and web site)
3) Products serviced
4) Area served (states, region, whatever)
I will organize and publish the full listing, with Nestor's blessing, on
this server.
Best to all,
Don Grimes, Microscopy Today

-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Friday, June 29, 2001 12:23 PM
To: 'service-at-jcnabity.com'
Cc: 'Microscopy Listserver'


Here's an old list, checked and amplified, that came to me from "Microscopy
Today"
I have checked all of these URL's

www.home.earthlink.net/~veilcs/ (EM+ special in Philips) (west Coast - USA)
www.southernmicro.com (Georgia) (Research LM)
www.dscoptical.com/services.htm (Boston?) (LM)
www.caleywhitmore.com (Boston?) (LM)
www.opticalexpertise.com (NY) (connection failure?)
www.mwrn.com/product/microscope/repair.htm (LM and EM)
www.sciscope.com/field-service.htm (30+ states) (up to Advanced LM)
www.svms.com/services/index.html (CA) (LM)
www.meyerinst.com (TX) (up to advanced LM)
www.microresource.com/services2.htm (IL) (LM)
www.allometrics.com/microscope_serv.htm (LA)(LM)
www.labworksinc.com (San Francisco) (Limited to specialized LM scope
accessories)
www.mikronet.com (San Diego) (general LM services)
www.uams.edu/oas/OES/oesilab.htm (AR) (University of Arkansaw LM support
facility)
www.uni.edu/pur/mms/equipment.html(U. N. Iowa) (Wide range of equipment
including EM's)
www.dominionmicroscope.com/services.htm (Richmond, VA) (LM and associated
equipment)
www.eosltd.co.uk/ (UK.COM)
www.emsys.co.uk/ (UK.COM)
www.hii.hitachi.com/svcem.htm (Hitachi)
www.leo-usa.com/ (LEO)
www.feic.com/products/index.htm (FEI - Philips)
www.jeol.com/locations/service/service.html (JEOL Service, USA)


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



From daemon Fri Jun 29 17:25:39 2001



From: Eric :      biology-at-ucla.edu
Date: Fri, 29 Jun 2001 15:24:31 -0700
Subject: UA ka ka on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ok, I can't resist... I haven't seen anyone mention the way I
learned to do it in 1962...

Cut lens paper into strips a bit wider and longer than the slide.
Place an end of the paper over the end of the slide and add a drop
or so of your favorite liquid to the paper on the slide...just
enough to spread over the width of the slide. Grasp the edge of
the paper and pull slowly down the length of the slide so that the
excess liquid is wicked into the remaining paper as it is pulled
across the slide. Try to run out of paper as you reach the other
end of the slide.

As Ever,
Orin Keplinger
----- Original Message -----
} From: "Tobias Baskin" {BaskinT-at-missouri.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, June 28, 2001 4:28 PM


Hi there fellow microscopists,

Here in the Pathology EM lab we do not do rocket science, but standard
thin-section TEM of renal biopsies.

In the last two weeks though I have been getting some ka ka (technical
term) on my grids from my UA stain. I have already made up two new batches
of UA stain and use .45 micron filters just before use. Question is that
all of the sudden I am getting UA stain artifact on my grids. I have not
have any trouble since I have been here.
I have changed everything, new staining plate, cleaned and scrubbed
everything that I use for staining. I still get some UA artifact. Is this
normal and should I just forget about it or is there something else wrong I
am missing now. I mix up a 2% UA stain of 50% methanol and 50% water. I
stain for usually 10-12 minutes. the grids before staining or clean, but
after UA stain they are a bit dirty...

Any suggestions?

Thanks,

Eric A. Rosen
UCLA Medical Center
Dept. Pathology
EM Lab


From daemon Fri Jun 29 17:34:52 2001



From: Teresa Flores :      tflore-at-lsuhsc.edu
Date: Fri, 29 Jun 2001 17:32:27 -0500
Subject: MT2&MT2B service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow colleagues,I am in a dilema. I just found out that RMC(whom our lab
had Maintenance Agreement on two MT2 and one MT2B) has been taken over by
first Ventana and at a later date Biobeck?
Later company is not offereing maintenance service contracts for "old" MT2
or MT2B. Is there any one out there that could refer me to someone who
works on these great workhorses in EM?
Thanking you in advance, Teresa

PS: Mr. Strickland, are you out there?


From daemon Fri Jun 29 17:37:40 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 29 Jun 2001 17:35:13 -0500
Subject: cleaning of oil immersion lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:
I've been following the discussion of lens cleaing and would like
insert my two cents worth. I'm from far outside of generally biological
tenor of this group; an industrial metallographer ( microscopy of metal and
alloys). After using an oil immersion lens, I clean it with Kodak lens
cleaner fluid after blotting up the excess oil with a lens tissue, also
Kodak. After 30 years of use, my lenses are clear and clean with no apparent
deterioration. I did use xylene at first but was dissuaded by knowledgeable
microscope salesman.

Sam Purdy
Technical Center
National Steel Corp.
Trenton MI


From daemon Fri Jun 29 17:44:44 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 29 Jun 2001 15:39:46 -0700 (PDT)
Subject: Electroscan E3 ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Many thanks to everyone for their helpful comments on the Electrscan E3
SEM. We are still having vacuum problems in wet mode only. It works fine
in High vacuum mode though. We checked the diffusion pumps, tried moving
a rotary pump closer to the column, but no improvement. We are slowly
changing o-rings to see if that helps.

Does anyone have any detailed repair manuals or diagrams with o-rings for
this instrument? We have the customer maintenance manual, but it is
rather limited. It mainly covers how to change apertures and doesn't go
too deep into troubleshooting the vacuum system.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Jun 29 21:12:15 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 29 Jun 2001 19:03:23 -0700
Subject: Re: Microscope magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't use this scope but I could venture the following guestimates.

If the photo system and light path does not include a separate
photo objective (photo eyepiece) then the film plane magnification
is the objective x 4. This would then be 20 x 4 = 80X.

Usually, with a 35mm camera body, the system is set up to
be 1:1 with the oculars. This would mean 20 x 10 = 200X. If this
field of view is different with the 4x5 cone, then there is some
intermediary lens element. Usually, it is a 2.5X objective.
For photo work, the oculars won't make any difference.

Perhaps some Optiphot experts can help you better.

gary g.


At 02:16 PM 6/29/2001, you wrote:

} Need some help trying to figure the magnification of some photos taken with
} the following:
}
} Nikon Optiphot-Pol Microscope
} CF eyepiece 10X
} CF objective 20X
}
} with Nikon AFX-II camera control assembly using a Polaroid 4x5 film holder
} with a 4X marked on it.
}
} Focus for images was done through the monocular eyepiece on the camera
} assembly.
}
} I assume that my magnification though the microscope eyepiece would be 30X
} I assume that the magnification to the camera back would be 24X
}
} Sorry if this seems like a silly question but I do not do much optical
} microscopy and got to thinking that it may be more than just the sum of the
} optics magnification.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu



From daemon Fri Jun 29 22:31:45 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 29 Jun 2001 22:25:40 -0500
Subject: UA ka ka on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

We have found staining artifacts to be one of the most frustrating,
intermittent mysteries of all. A while back we began having trouble with UA
and Pb contamination where no trouble had occured before---same stains, same
processing, same everything, but suddenly there was goop everywhere. We
mixed new stains, changed all of our 0.2 um filters, spun everything down,
and filtered again, used NaOH religiously, held our breath while staining,
etc., etc., etc. Finally, the only thing we could think of was water, so we
called in our water system maintainer (let's just call them "Hey Mystery
Man!!!!) who had been servicing our reverse osmosis, deionization system
forever.

Well, they said, your system's shot. The diaphragm's bad, the cartridges
are way expired, and the filter is one big bucket of rust. You need to
maintain this better. We informed them that we paid them a princely sum to
maintain it for us and what the h... were they doing every month when they
came in to fuss with it?

Turns out that they thought we owned the system and had only been swapping
out some tank or other, and they assumed we were doing the rest. When we
produced documentation of our service contract on their leased system that
we had been paying on since about 1735 A.D., there was an awkward pause.
Then we fired them.

We purchased a Millipore tabletop water purification system that solved our
problems overnight. It's a small, low-volume gizmo that produces a few
litres of ultra-high purity water a day. Costs about $1200, as I recall,
and requires annual filter changes to the tune of $200-300.

Moral of the story: Check the water when all else fails. Worked for us,
anyway. Couldn't hurt to check.

We have no stake in Millipore whatsoever, except as relieved users of one of
their products.

Randy Tindall
Electron Microscopy Core
University of Missouri



-----Original Message-----
} From: Eric
To: 'Microscopy Listserver'
Sent: 6/29/01 5:24 PM


Hi there fellow microscopists,

Here in the Pathology EM lab we do not do rocket science, but standard
thin-section TEM of renal biopsies.

In the last two weeks though I have been getting some ka ka (technical
term) on my grids from my UA stain. I have already made up two new
batches
of UA stain and use .45 micron filters just before use. Question is
that
all of the sudden I am getting UA stain artifact on my grids. I have
not
have any trouble since I have been here.
I have changed everything, new staining plate, cleaned and scrubbed
everything that I use for staining. I still get some UA artifact. Is
this
normal and should I just forget about it or is there something else
wrong I
am missing now. I mix up a 2% UA stain of 50% methanol and 50% water.
I
stain for usually 10-12 minutes. the grids before staining or clean,
but
after UA stain they are a bit dirty...

Any suggestions?

Thanks,

Eric A. Rosen
UCLA Medical Center
Dept. Pathology
EM Lab


From daemon Sat Jun 30 02:17:47 2001



From: Feng Wu :      fwu-at-bgumail.bgu.ac.il
Date: Sat, 30 Jun 2001 10:06:19 +0300
Subject: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

I met some problems during I prepared the samples for GaN/sapphire plan-view
using tripod wedge technique. After polishing, the sample was thinned by pips
in one side. However, The other side usually was covered by copper, as a result
the sample became dark. It seems the unthinned side should be covered something
to prevent sputtered materials from growing on it. Do you have some knowledge
on it?

Thanks in advance.

Best regards.

Feng
**********************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

voice 972-8-6461473
fax 972-8-6472944
fwu-at-bgumail.bgu.ac.il
**********************************************




From daemon Sat Jun 30 08:36:31 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Sat, 30 Jun 2001 09:25:39 -0400 (EDT)
Subject: Re: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mica! 1stly core drill a 3mm disc. Then use sharp tweezers to peel a thin
piece from dozens or even hundreds of layers in the mica disc depending
how good you are to separate them. Anyway you just need one thin piece to
cover the other side, and you may be able to reuse that thin piece.

*********************************
Chaoying Ni, PhD
Electron Microscopy Laboratory
Materials Science and Engineering
College of Engineering
University of Delaware
Newark, DE 19716
*********************************

On Sat, 30 Jun 2001, Feng Wu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} I met some problems during I prepared the samples for GaN/sapphire plan-view
} using tripod wedge technique. After polishing, the sample was thinned by pips
} in one side. However, The other side usually was covered by copper, as a result
} the sample became dark. It seems the unthinned side should be covered something
} to prevent sputtered materials from growing on it. Do you have some knowledge
} on it?
}
} Thanks in advance.
}
} Best regards.
}
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
} fax 972-8-6472944
} fwu-at-bgumail.bgu.ac.il
} **********************************************
}
}
}



From daemon Sat Jun 30 08:55:12 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 30 Jun 2001 09:50:03 -0500
Subject: Surface protectant when thinning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Dr. Feng Wu wrote:
===============================================
I met some problems during I prepared the samples for GaN/sapphire plan-view
using tripod wedge technique. After polishing, the sample was thinned by
pips in one side. However, The other side usually was covered by copper, as
a result the sample became dark. It seems the unthinned side should be
covered something to prevent sputtered materials from growing on it. Do you
have some knowledge on it?
=================================================
There are two materials that to the degree we have been able to determine,
are essentially equivalent for this purpose: One is called Microshield™ and
the other Lacomit™.

SPI Supplies has offered Microshield for this purpose and more information
about its use and application can be found on the SPI Supplies website at
URL
http://www.2spi.com/catalog/chem/microshield.html

Disclaimer: SPI Supplies has not done any careful analysis to prove that
Microshield and Lacomit are identical, only that for this particular TEM
application, they do seem to be equivalent. If there is information to the
contrary, we would appreciate hearing about it. Suggestion: Use in
conjunction with the Microshield remover.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
===============================================


From daemon Sat Jun 30 09:20:18 2001



From: David Burton :      dburton-at-nwlink.com
Date: Sat, 30 Jun 2001 07:17:03 -0700
Subject: Re: Microscope magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Need some help trying to figure the magnification of some photos taken
with
} the following:
}
} Nikon Optiphot-Pol Microscope
} CF eyepiece 10X
} CF objective 20X
}
Hi Roy,


If you still have access to the microscope, put a specimen on the stage
which has a large feature, one you can accurately measure. Use the lowest
power lens available. Remove the film cassette from the camera and stretch
a piece of Scotch Magic tape across the film plane. Focus on the specimen,
lower the room lights and measure the size of the image on the scotch tape,
which will now be clearly visible. You could also measure some feature on
the original slide and compare it to the photograph.

I would guess the mag factor is 80x, but you can confirm that using this
process.

Dave Burton
Optical Specialist
University of Washington



From daemon Sat Jun 30 14:18:53 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 30 Jun 2001 15:11:01 -0500
Subject: Mat. Sci. TEM Sample Prep: Silicon nitride membrane window grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Dr. Andreas Taubert asked:
=============================================
this is some kind of follow-up question to Thearith's grid problem. We are
looking at about 2 nm features in a polymer matrix. The features have very
low contrast with respect to the all-carbon matrix due to the fact that they
mostly contain O and a little Zn. For sample prep reasons we need to support
the samples. Now: Are SiO and Si nitride coatings thin enough to still see
such things ? The available thicknesses of these films seem very large
compared to conventional C films.
=============================================
If the thinnest thickness of the nitride should still be too much, which is
presently 30 nm, but soon to be reduced to the 10-20 nm range, the present
silicon nitride membrane grids can be furnished "holey", that is, with a
matrix of holes that are about as small as you want them, in just about any
arrangement that you might want them. In that kind of situation, there is
no nitride underneath the sample being analyzed, so long as you are looking
"through" the holes. The only carbon (or oxygen) present is that which is
coming from the sample. The typical compliment of holes approximately
doubles the cost of the grid, because it is a separate operation done by
micromachining techniques. This is explained in greater detail at URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html

Disclaimer: SPI Supplies offers silicon nitride membrane window grids, both
with and without the holes.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==============================================


From daemon Sat Jun 30 19:15:39 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 1 Jul 2001 10:09:28 +1000
Subject: RE: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The ion beam forms a cloud of debris, which settles on the reverse side of the
specimen and this needs to be covered. A double beam ion gun system avoid that
problem because the specimen is thinned on both sides simultaneously.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, June 30, 2001 5:06 PM, Feng Wu [SMTP:fwu-at-bgumail.bgu.ac.il] wrote:
}
} Dear All,
}
} I met some problems during I prepared the samples for GaN/sapphire plan-view
} using tripod wedge technique. After polishing, the sample was thinned by pips
}
} in one side. However, The other side usually was covered by copper, as a
} result
} the sample became dark. It seems the unthinned side should be covered
} something
} to prevent sputtered materials from growing on it. Do you have some knowledge
}
} on it?
}
} Thanks in advance.
}
} Best regards.
}
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
} fax 972-8-6472944
} fwu-at-bgumail.bgu.ac.il
} **********************************************


From daemon Sat Jun 30 22:12:56 2001



From: sterling stoudenmire :      sstouden-at-thelinks.com
Date: Sat, 30 Jun 2001 22:26:45 -0500
Subject: scintillation crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i am trying to find a way into the literature on experimental techniques
appropriate to study 511 photon detection and capture vs properties of
scintillating materials. Is there a standard way to do this?

Can someone point me at a good place to begin a literature survey?'
sorry, my question is not better phrased, but I am just beginning to look
at this?

sterling


Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find
the cure for cancer and other diseases. There will always be a need for
the trained clinician (MD/RN) but, advanced diagnostic and treatment option
selection has become gene based, has moved from the physician's practice to
the computerized cell and molecular biology laboratory, and appropriate
treatment options should now be based on the personal biology of the
patient.


From daemon Mon Jul 2 05:29:14 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Mon, 02 Jul 2001 11:02:41 +0000 (GMT)
Subject: Re: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Feng,
the trick is not to have a hole in your specimen where the ions can go through and back-sputter the Cu on to your sample. So make your specimen big enough to cover up the hole in the support grid and stop ion milling immediately when a hole appears. The problem will go away to some extent if you use the two-sided specimen holder. Alternatively, use an inert grid and dissolve the Cu with an acid after specimen prep. (Most acids are unlikely to affect GaN or sapphire.)

Richard




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} I met some problems during I prepared the samples for GaN/sapphire plan-view
} using tripod wedge technique. After polishing, the sample was thinned by pips
} in one side. However, The other side usually was covered by copper, as a result
} the sample became dark. It seems the unthinned side should be covered something
} to prevent sputtered materials from growing on it. Do you have some knowledge
} on it?
}
} Thanks in advance.
}
} Best regards.
}
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
} fax 972-8-6472944
} fwu-at-bgumail.bgu.ac.il
} **********************************************
}
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street London
W1J 6AQ






From daemon Mon Jul 2 05:56:25 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 2 Jul 2001 11:44:01 +0100 (GMT Daylight Time)
Subject: Re: UA ka ka on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From reading posts on this listserver I gather the problem
is usually related to a failure of the water purification
system. If you use purified water why not make some UA up
in double distilled water as a check.

Dave


On Fri, 29 Jun 2001 15:24:31 -0700 Eric {biology-at-ucla.edu}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there fellow microscopists,
}
} Here in the Pathology EM lab we do not do rocket science, but standard
} thin-section TEM of renal biopsies.
}
} In the last two weeks though I have been getting some ka ka (technical
} term) on my grids from my UA stain. I have already made up two new batches
} of UA stain and use .45 micron filters just before use. Question is that
} all of the sudden I am getting UA stain artifact on my grids. I have not
} have any trouble since I have been here.
} I have changed everything, new staining plate, cleaned and scrubbed
} everything that I use for staining. I still get some UA artifact. Is this
} normal and should I just forget about it or is there something else wrong I
} am missing now. I mix up a 2% UA stain of 50% methanol and 50% water. I
} stain for usually 10-12 minutes. the grids before staining or clean, but
} after UA stain they are a bit dirty...
}
} Any suggestions?
}
} Thanks,
}
} Eric A. Rosen
} UCLA Medical Center
} Dept. Pathology
} EM Lab
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Jul 2 06:48:44 2001



From: arunachalam rajagopal :      ar_rajagopal-at-hotmail.com
Date: Mon, 02 Jul 2001 17:11:12 +0530
Subject: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
We have a problem of saw toothe edges in JEOL 5410 SEm in scan 1 mode after
x5000 Magnification.We tried changing OL,Cl aperture,Reducing filament
spacer to 2.0mm.But no reults.The picture in slow scan is fine up to x20k.In
higher Magnification we couldn't focus the image properely.(the sample used
sputtered gold).Expecting everybody suggestion.Thanks in advance.

Regards,

A.Rajagopal
Blue star Ltd
Bangalore
India.
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.



From daemon Mon Jul 2 08:56:44 2001



From: Micha Bayer :      M.Bayer-at-rbge.org.uk
Date: Mon, 2 Jul 2001 14:52:35 BST
Subject: DPX versus Canada Balsam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I am in the process of figuring out a way of permanently mounting
desmids (unicellular green algae with a cellulose cell wall) and was
wondering about the relative benefits of DPX and Canada balsam as
mounting media.

It appears that no-one in our field has done this kind of thing before
(please tell me if I am wrong) and we have to guess our way through
this.

Is Canada balsam still used? Which mountant has the better long
term and optical characteristics in your experience? Has anyone got
DPX slides which are older than say 20-30 years, and are they still
acceptable?

many thanks

Micha Bayer

______________________________

Dr. Micha Bayer
Royal Botanic Garden Edinburgh
20A Inverleith Row
Edinburgh EH3 5LR
Scotland, U.K.
Tel. (+44) (0)131-248 2915 or 248 2965
Fax (+44) (0)131-248 2901
Project Homepage at http://www.rbge.org.uk/ADIAC/
RBGE home page at http://www.rbge.org.uk
______________________________


From daemon Mon Jul 2 08:56:45 2001



From: wft03-at-health.state.ny.us
Date: Mon, 2 Jul 2001 09:48:40 -0400
Subject: Re: scintillation crystals

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i am trying to find a way into the literature on experimental techniques
appropriate to study 511 photon detection and capture vs properties of
scintillating materials. Is there a standard way to do this?

Can someone point me at a good place to begin a literature survey?'
sorry, my question is not better phrased, but I am just beginning to look
at this?

Dear Sterling,
It's been a while since I worked with these kinds of detector. You
may be interested in either of two different types, depending on what the
experiment is. Alkali halide scintillators are inexpensive and can be made
large enough to cover a big solid angle (so two of them in coincidence can
register the positron annihillation photons), but their energy resolution
is rather poor. Germanium solid-state detectors are more expensive, but
also can be made to cover a big solid angle to detect photon pairs, and
their energy resolution is excellent. Find as up-to-date text on nuclear
physics as you can, and check out the referrences from the chapter on
detectors. Also check out the web for manufacturers of these
detectors--Ortek and Canberra are the two biggest. Both companies have
technical manuals which describe the detection process. They carry the
solid state detectors and may also carry alkali halide detectors (or know
who does). Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Jul 2 09:28:26 2001



From: Julian Martinez-Fernandez :      Julian.Martinez-Fernandez-at-grc.nasa.gov
Date: Mon, 02 Jul 2001 10:21:37 -0400
Subject: Summary on the information about Imaging plate systems for TEM

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Here you have a summary on the information about IP systems for TEM. Thanks
to everybody for the help!

Regards,
Julian


Their linearity is over 5 orders of magnitude as opposed to 2 orders with
film. However their resolution is much poorer. Something like 50 to 100
microns versus 2 microns with film.

bob j


I believe the pixel size is 30micron instead of film's 5u, so you cannot
enlarge as much subsequently (or you need to use 6X more mag for same
enlargement capability, and end up with 1/36 of area).


On a general way, the interrest of the IP is that its reponse in linear on
a wider domaine than the Ag film. So you can enhance contrast if you have
a small contrats in your sample (biology), or you can take a big dynamic
in one exposure (for exemple in CBED mode).

The harware is expensive when you buy it, but the use is from no cost
(apart the computer ; images are more than 20 Mb big !).

We use it, but we use also the film, and we will not stop the film soon.

But contact J. Werckmann. He can say much more about it than I.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France


I did study this subject a few years ago and find that this technique is
not well optimized for EM. For many reasons:

- cost of the system is similar to the EM digital camera
- you require to load/unload Imaging Plates into the cassettes (you waste
the time)
- it takes from 2 to 5 min to scan one Image Plate (waste time)
- I am not sure exactly, but seems to me, that you have to scan Image
Plates soon after tacking picture, otherwise the image will deteriorated,
and seems to me they are sensitive to light too.
- you don't have a chance to adjust your image before recording (as it
happens in case of digital cameras).
-number of "shots" is limited and Image Plates are princely
- Image Plates may be damaged during loading/unloading which may reduce
declared number of use (a few thousand times I believe).

The good things about Image Plates are:
- Image plate has more pixels than EM digital cameras, so the resolution is
better.
- you don't have to make any changes in the microscope.
- the linearity and dynamic range is similar to the EM digital cameras
- easy to use and less problem related to the equipment.

My conclusion was: technically, it's a great stuff, but it does not help in
practice. You still need some help from technician to load/unload plates
and scan them and you have to pay a 20-40$K for that beauty. Not so
promising technology for TEM at least.

This information based on my "study" performed a few years ago and may not
reflect modern improvements in this area.

Sergey.

All digital cameras including Gatans are approximately 10 times more
sensitive than 4489 film. I know it for sure because just returned back
from Gatan's demonstration. Image Plate should have very similar
characteristics in terms of sensitivity, dynamic range and linearity as
modern digital cameras. The best things about Image Plates it's their pixel
size: 5000 x 4500 total pixels per plate. It's greater than in digital
cameras: 1000x1000/2000 in most cases. It's still less than you could get
from film with $800 scanner: 6400x4800 for 1600 optical dpi.
BUT!!!
You have LOAD/UNLOAD plates into cassettes manually. In the dark. Stack
them and load into the scanner (so, you have to have space for the scanner
in dark-room). Scanner will process them automatically (and at that time
you could not use dark-room for regular things, light from scanner). Load
them back into cassettes, degas magazine and load it into microscope. I
don't remember exactly, but it seems to me the cost of one plate is
something around $100. So, I have two magazines with 50 cassettes each:
100x100$ plus scanner plus salary for technician plus computer and storage
media. Ok, I forgot to mention that you have to ERASE plates before use,
also, using special eraser. I don't remember, how many plates you could
ERASE in one run. May be it's automatic too. Scanning: 3.5 min per plate x
50= 3 h.

As I mentioned before, the technique is great, but not so helpful for
regular day-to-day basis EM and does not save time so much. It works fine
for molecular biology applications in "phospho-imagers" but people need to
scan a few plates per day (even one a week) and they save a lot of time:
10-15 min exposure vs 5-10 days! I just don't see reason to switch to this
technique to myself. If I'll have such money, I would buy digital camera.
It's more practical solution from my point of view.

Sergey

There are advantages and disadvantages to all imaging systems. In some
instances
CCD cameras are preferable to film, in other instances film is superior
(especially if wide field of view is a factor). What is best to use
largely depends on the details of your experiment. I thought I would
provide some information on imaging plate systems, and indicate one
strong advantage the technology has over other recording media/systems.

Image plate systems use the "photostimulated luminescence" (PSL)
phenomenon. Regions in PSL materials that have been previously exposed
to a short wave length irradiation, will luminesce when stimulated by a
second longer wavelength irradiation. The stored energy is stable until
released by the second irradiation. Imaging plates are made using PSL
films of barium fluorobromide (= 5 µm grain size), containing a trace
amount of bivalent europium as a luminescence center, which are
uniformly coated on a polyester support. Imaging plates replace
conventional photoemulsion film in the transmission electron microscope
and are exposed the same as normal film. To read the recorded image,
the imaging plate is latter scanned by a red diode laser and the
luminescence is collected through a light collection guide to the
photo-multiplier tube (PMT). The analog current produced by the PMT is
converted to a 8 to 16 bit depth digital signal. The pixel density can
be read at between 50 to 400 pixels/cm. Imaging plates can be fully
erased for reuse, but have a finite lifetime.

One main advantage of PSL films in their ultrahigh sensitivity (e.g. 2
x 10-14 - 2 x 10-9 C-cm-2 at 100 kV) to electron irradiation making them
valuable for biological work (these are values quoted by a manufacturer
Fuji). For example, film emulsions require 0.1 to 1 C-cm-2 to record an
optical density S = 1 at 100 k magnification (see Reimer, 1975). Slow
scan CCD cameras offer better sensitivity than film, however are
inadequate for imaging certain biological specimens. Furthermore,
CCDcameras have limited fields of view as compared to film. As an
example, Sugi (1997) and coworkers have used a Fuji imaging plate system
to record micrographs of myosin head movement induced by ATP hydrolysis
in an environmental cell TEM. I avoided using the term “living myosin”
since a biomolecule is not strictly alive, but it was biologically
active. Electron doses low enough not to damage the delicate
biomolecule could be used to record images on an imaging plate.

Reimer, L. (1975) in Physical Aspects of Electron Microscopy and
Microbeam Analysis, eds B. M. Siegel, and D. R. Beaman, John Wiley &
Sons Publishers, New York, New York, 231-245.

Sugi, H., Akimoto, T., Sutoh, K., Chaen, S., Oishi, N., and Suzuki, S.
(1997) Proc. Natl. Acad. Sci. USA 94, 4378-4382.
I contacted ditabis several months ago. Beyond a confirmation email from
the company, I haven't received any from them.

Steve Widing
Temple University

The Image plate system is awesome for materials applications as well.... !!

It is very easy to obtain diffraction patterns that include varying
intensities (e.g., a spot pattern mixed with Kikuchi lines) with just one
exposure on a single plate. The plates also show the slightest deviations
in contrast which are not discernable using film, a CCD camera, or the TEM
viewing screen. For example, differences in contrast in amorphous silicon
oxide due to the multiple processing steps in integrated circuits may be
easily observed with the plates. The advantage in the plates is evident in
the low to intermediate magnification ranges where large variations in
image contrast can be manipulated off-line. Each IP file is quite large
(25MB) and therefore, contains plenty of information which can be
manipulated before reducing its size that is suitable for publication
purposes. The disadvantages to the IPs are the cost (although I believe
the cost is dropping) and the extra processing time needed (~ 80 minutes
for a batch of 32 plates).

The CCD camera is still very useful in the high resolution imaging regime
where the intensity is rather uniform throughout the field of view and one
can instantly observe the quality of the image. We will be evaluating high
resolution CCD images with high resolution images obtained with the IPs in
the near future. We are not sure what effect (if any) vibrations from the
film mechanism will have on the high resolution images.

*******************************************************************
Lucille A. Giannuzzi, Ph.D.
Associate Professor, Mechanical, Materials, and Aerospace
EngineeringDirector, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305Orlando, FL 32826email lag-at-mail.ucf.edu
phone (407) 882-1500
fax (407) 275-4321

We have just been testing the Ditabis IP scanner and were impressed.

It doesnt quite replace film or I think a good small-pixel below-screen CCD
camera for resolution, but is much better than a whole field above screen
CCD camera for resolution, and for dynamic range is in a class by itself.

We tested with diffraction patterns showing bright and dim diffuse
features, convergent beam patterns, wedge-profile ion beam thinned
sections, and normal and unstained biological material.

For low dose work, 1-2 seconds exposure at beam intensities barely visible
to the eye was no problem. The resolution problem may be avoidable in
practice because images may be taken at higher magnifications and lower
beam intensities than normal for film - that is just a question of getting
used to matching the imaging conditions to a different medium.

PLate handling is easy. The plates are loaded into the TEM magazine in the
light. AFter electron exposure they are kept dark, but loaded into the
scanner under light as dim as convenient - we used the light from the
monitor screen.

Storing the plates for up to five days made only a very small difference to
the intensity histogram - we didnt test beyond that. Plates can also be
rescanned with very little loss.

The only real inconvienience we found was the impossibility of recording
plate number, mag etc on the IP plate, as we are used to on the negative.
If we had our own system we would mark the plates permanently in some way
that showed on the readout.

IP plates are complementary to CCD cameras in some respects. You dont get
the advantages of immediate viewing, but more dynamic range, more pixels,
and of course one scanner can take plates from any number of TEMs.
Finally, the price was the sticking point for us at this time. But if a
cooperative lab somewhere else on this continent gets one we would likely
buy a set of plates and work through the postal system until the price
drops or somebody comes up with a "must have" application!

regards
Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU






From daemon Mon Jul 2 09:49:30 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 2 Jul 2001 10:43:06 -0400
Subject: RE: Microscope magnification

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Dear Roy,
Even worse than that. It's almost trigonometry, and the great
unknown, tube length! The best way to determine the mag of photos already
done, for which you know the ocular and objective combination, is to go back
to the scope with a specimen of known dimensions - I use something called a
stage micrometer - a micro-ruler on a slide that has gradations down to
10um. I have photos (rulers) for every combination on my scope. I do the
same for the CCD camera I sometimes put on my microscope in place of the
film camera. My suggestion is to go down(over) to the department of Biology
and see if someone can loan you a "Stage Micrometer".

Regards,

Fred Monson

} ----------
} From: Beavers, Roy
} Sent: Friday, June 29, 2001 5:16 PM
} To: 'Microscopy Listserver'
} Subject: Microscope magnification
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Need some help trying to figure the magnification of some photos taken
} with
} the following:
}
} Nikon Optiphot-Pol Microscope
} CF eyepiece 10X
} CF objective 20X
}
} with Nikon AFX-II camera control assembly using a Polaroid 4x5 film holder
} with a 4X marked on it.
}
} Focus for images was done through the monocular eyepiece on the camera
} assembly.
}
} I assume that my magnification though the microscope eyepiece would be 30X
} I assume that the magnification to the camera back would be 24X
}
} Sorry if this seems like a silly question but I do not do much optical
} microscopy and got to thinking that it may be more than just the sum of
} the
} optics magnification.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu
}
}
}


From daemon Mon Jul 2 09:56:12 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 2 Jul 2001 10:49:38 -0400
Subject: RE: RE: long-term storage OR what has happened?

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Sorry, I got carried awazy.

FCM


} ----------
} From: Barbara Plowman
} Sent: Friday, June 29, 2001 5:46 PM
} To: fmonson-at-wcupa.edu
} Subject: Re: RE: long-term storage OR what has happened?
}
} Excuse me, but might you need your medication? Enough...
}
} } } } "Monson, Frederick C." {fmonson-at-wcupa.edu} 06/29 7:30 AM } } }
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} You are absolutely right, and most of the time we behave just as you
} describe us. Patient, etc. One purpose of my tirade was to point out
} that
} there are differences between today and yesterday that give ALL of us
} choices to make. I know a high school student who failed the year,
} because
} he wanted to punish his parents. When he told me what he had done, I
} responded that I knew a young man just like him when I was in high school.
} His solution was to get straight A's so he could become more quickly
} independent.
} Science is our own, very human, endeavor. Those of us who do
} science must constantly work to remove the temptations to embellish our
} results, to twist the statistics and to 'jump' to conclusions. But, and
} here's where I differ perhaps slightly. Sometimes, there is a good reason
} to yell! My wife hates my streak of sarcasm! But I was raised on the
} stuff. When I did something 'dumb,' everyone who loved me, joined in the
} derisive repair. I learned to think at my dinner table before I was 10.
} I
} did not like to be the but of everyone's attention. My Mom taught, then I
} taught. I only stopped, because no one [too few] wanted to learn any more
} [bad generalization]! Go to college today, and what do you find? Young
} people unprepared for the rigor. Young people unprepared for the
} intellectual dance - as we used to call it. Young people who don't know
} what they want to do and are unwilling to work to find out. "They only do
} what they are taught!" This is my mantra when I look for explanations in
} the mirror. My older son had to learn - for the test - to name NH3 as
} "ammonium", because his chemistry teacher would not believe the Handbook
} of
} Chemistry and Physics. My chemistry teacher in public high school took
} chemistry courses at Rutgers every third summer. He was the expert in
} chemistry. My physics teacher had a PhD in the subject. My biology
} teacher
} went on to be the chair of Physiology at a large Southern medical school.
} If I wanted to complain about a professor at Lehigh, the Dean would show
} me
} a list of nearby schools that had openings. The attitude was clearly
} communicated. The greatest lessons in life are those that teach one to
} manage the worst parts of it. If I failed a course, it was MY failure -
} and
} it was at least MY problem! I wanted to be what I am, once I figured
} everything out. The only advice I ever received, when I was having
} problems
} with learning, was, "Study harder." My Mom just kept on telling me to
} keep
} on trying as hard as I could. If I were young now, I'd be in group
} therapy
} and zapped on some pharmaceutical, if the system had its way.
} Given my experience, I hope it is not difficult to understand why I
} might not appear to understand the problems of others. BUT! If I see a
} young person in science who is poorly informed about how it is done, then
} I
} will act in that person's behalf even if I am thought, and reported, to be
} insensitive.
} When I was a graduate student, we were all ordered to write
} faculty/course evaluations. I did. I wrote analyses of each course, and
} every one of them was critically positive. Not one word of my analyses
} were
} printed. If I had demonized one of my professors, I would have been
} writing
} novels today. The people who ordered the writings were ex-faculty
} administrators who were pandering to the new consumer mentality in
} education. If I had gone to my undergraduate department chair to complain
} about a grade, he would have told me that I was wasting his time. Five
} years later, there were lines of boomers outside of department offices.
} The
} generation of manipulators had arrived! While they were standing in line,
} I
} was learning. While I was learning, they were just getting warmed up!
} I have had ideas snitched! Sometimes I even gave them away. On the
} other hand, I have also had the other side of the experience. For six
} months of learning followed by 30 minutes of consultation and work, I had
} the good fortune to become a molecular biologist. For that effort, the
} individual to whom I gave my best remembered my part - even after he had
} spent years doing the work - and included me in the author list for a
} gene(TERE1) that mapped to Chromosome 1 and appeared in print in a Nature
} publication. He did NOT have to do that. There was NO obligation nor
} agreement. I helped because he asked. People are basically good,
} especially scientists, and I always start with TRUST, and I always will.
} You are still absolutely correct.
}
} Regards for YOUR sensitivity,
}
} Fred Monson
}
} } ----------
} } From: Sousan Abolhassani
} } Sent: Thursday, June 28, 2001 8:07 AM
} } To: Paul Webster
} } Cc: MSA listserver submission
} } Subject: Re: long-term storage OR what has happened?
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
}
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} } -----------------------------------------------------------------------.
} }
} }
} } Paul,
} }
} } The point about this server is that it is used by
} } a very heterogeneous public from which a non-exclusive
} } list is the following:
} }
} } students,
} } professors,
} } scientists/
} } researchers
} } technical staffs
} } suppliers
} } producers
} } specialists
} } non-specialists
} } etc.
} } etc.
} } etc.
} }
} } and all these from so many different backgrounds with so
} } many different problems and even from so many different countries...
} } That if we want to teach all of them to behave as we expect
} } before they post a query, the server could go to early retirement.
} }
} } I guess the philosophical attitude that one should have is to
} } really try and understand both sides of the discussion.
} }
} } 1- on one hand the trust that professor Monson is demanding
} } is not there because our users are a small sample of our
} } society. How many times have we heard that people have had
} } their ideas picked by others and their publications stolen
} } by others and .... in the very scientific community that most
} } of us hoped to be the purest part of the society. Still
} } the scientists are generally speaking very sincere people.
} } So if someone is not having trust to others, it's the
} } consequence of what they have observed in their environment.
} }
} } 2- I do not know if Rachel is a student (may be not), and if she
} } really was not trusting or just did not think to write the reason and
} } so on, so we do not discuss the case on a specific way.
} } But talking about going to the library, there are labs
} } where the students have really not access to the books and there
} } are places where superiors do not give the support the students
} } and other researchers' need, or simply there are not
} } enough books available.
} }
} } So there are always reasons for the way people behave
} } and the more our society goes towards a very materialistic and
} } "win whatever the situation" attitude, the more the arguments
} } of professor Manson will be applicable because the more people
} } will behave in the way he is not happy about, and I understand
} } and apreciate his point.
} }
} } All that apart, it is better to listen to people,as often things
} } that are not to be done are not published and are only "known".
} } Of course provided that answers are given generously, as arrogance
} } for giving is so destructive, even for donator.
} } I therefore agree with the fact that we should just be able to
} } ask the question and someone will react to give us an answer.
} }
} } I think that professor Monson has evoked a point that is of
} } great importance and that is the human aspect of all we do, as
} } "human" is what we are hoping to be at a first place.
} } That needs sensitivity... and continuous effort.
} }
} } Regards,
} }
} } Sousan
} }
} }
} } Paul Webster wrote:
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } } Dear Colleagues,
} } }
} } } The messages from Professor Monson in reply to a question about
} } long-term
} } } tissue storage makes me wonder what has happened to this listserver?
} } }
} } } His message was highly entertaining and even if the poster of the
} } original
} } } message may have felt "picked on", there was no reason to expect him
} to
} } post
} } } an apology, no matter how informative it was.
} } }
} } } Far too many posters use this listserver as a way of finding a quick
} fix
} } to
} } } simple solutions to technical problems that can be easily researched
} in
} } text
} } } books.
} } }
} } } Often these messages contain little background information on the
} } technical
} } } problem under investigation. Even so, many of these questions get
} full
} } } attention and detailed replies, even if the replies are not posted on
} } the
} } } main list for public view.
} } }
} } } What has happened to the free and open discussion of issues and
} } protocols
} } } that we used to have? Are people so afraid of being accused of being
} } } incorrect that they do not post their replies openly? Or maybe people
} } are
} } } afraid of being accused of being impolite.
} } }
} } } I look forward to controversy and hope that my comments are read
} } carefully
} } } and replied to with vigor. Only in this way will I be able to learn
} new
} } } thing, and this is the main reason I still subscribe to this
} listserver.
} } }
} } } If I am factually wrong about something, tell me. It is better to
} find
} } this
} } } out on the listserver than in a more professional (or cruel)
} } environment.
} } }
} } } As for discussion subjects: why was there no comment about the
} } unadvisable
} } } suggestion made about storing samples in a frozen state? Would anyone
} } } working with EM really choose to store their samples at -20 degrees
} } before
} } } processing for TEM?
} } }
} } } Where is all the discussion about extraction that can occur when
} storing
} } } samples in alcohols or buffers, or on the contamination that I know
} } grows in
} } } everyones old cacodylate buffers?
} } }
} } } Do we not comment because we know it has all been published in
} } textbooks?
} } }
} } } Dear Profesor Monson, do not feel bad about improving our general
} } knowledge
} } } and our professional etiquette. I for one support your views and find
} } your
} } } comments useful and entertaining. Please keep up your submissions.
} } }
} } } Best regards,
} } }
} } } Paul Webster
} } }
} } } Paul Webster, Ph.D.
} } } Scientist II and Director
} } } Ahmanson Center for Advanced EM & Imaging
} } } House Ear Institute
} } } 2100 West 3rd Street
} } } Los Angeles
} } } CA 90057
} } }
} } } pwebster-at-hei.org
} } } p: 213 273 8026
} } } f: 213 413 6739
} } } http://www.hei.org/research/depts/aemi/aemi.htm
} }
} }
}
}


From daemon Mon Jul 2 10:12:03 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Friday, June 29, 2001 5:24 PM
Subject: Fwd: UA ka ka on grids

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Eric,
A couple things to check:
(1) 12 min staining may be longer than necessary....try 5 min and see what happens.

(2) What about the water used for rinsing? This can be a source of contamination. Always use double distilled water. I used to use the bottled water used for reconstituting vaccines when I worked in a medical center and had easy access to it. At the time I had a less than reliable water source.

(3) Try washing by moving your grids through a series of water droplets prior to "jet" washing with a squirt bottle. This eliminates retension of stain along grid wires.

(4) Are you sure you are not getting contamination from phosphate percipitation (you didn't say what fixative you used for your material). If the sample has a high calcium content and is fixed with a phosphate buffer, it can lead to percipitation of a fine electron-dense compound....especially if the tissue also has high lipid concentration.

I would run some sections from blocks containing other tissues that have worked well in the past as a method control. If they are clean and your problem sample is not, then look for the dirt source prior to the staining step.
Good luck...these problems are a never ending source of frustration.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907


--------------------------------------


Hi there fellow microscopists,

Here in the Pathology EM lab we do not do rocket science, but standard
thin-section TEM of renal biopsies.

In the last two weeks though I have been getting some ka ka (technical
term) on my grids from my UA stain. I have already made up two new batches
of UA stain and use .45 micron filters just before use. Question is that
all of the sudden I am getting UA stain artifact on my grids. I have not
have any trouble since I have been here.
I have changed everything, new staining plate, cleaned and scrubbed
everything that I use for staining. I still get some UA artifact. Is this
normal and should I just forget about it or is there something else wrong I
am missing now. I mix up a 2% UA stain of 50% methanol and 50% water. I
stain for usually 10-12 minutes. the grids before staining or clean, but
after UA stain they are a bit dirty...

Any suggestions?

Thanks,

Eric A. Rosen
UCLA Medical Center
Dept. Pathology
EM Lab




From daemon Mon Jul 2 10:15:31 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Friday, June 29, 2001 4:16 PM
Subject: Fwd: Microscope magnification

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Roy,
Invest in a good micrometer slide and use it to determine magnification. It may seem expensive initially but, since it can be used on any coumpound microscope regardless of set-up and camera, it will pay for itself in convenience (and accuracy of determining magnifications) for years to come. You can purchase them through your microscope venders for both compound and stereo microscopes.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907

--------------------------------------


Need some help trying to figure the magnification of some photos taken with
the following:

Nikon Optiphot-Pol Microscope
CF eyepiece 10X
CF objective 20X

with Nikon AFX-II camera control assembly using a Polaroid 4x5 film holder
with a 4X marked on it.

Focus for images was done through the monocular eyepiece on the camera
assembly.

I assume that my magnification though the microscope eyepiece would be 30X
I assume that the magnification to the camera back would be 24X

Sorry if this seems like a silly question but I do not do much optical
microscopy and got to thinking that it may be more than just the sum of the
optics magnification.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu





From daemon Mon Jul 2 10:45:52 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 2 Jul 2001 11:36:18 -0400
Subject: Re: Microscope magnification

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At 4:16 PM -0500 6/29/01, Beavers, Roy wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Jul 2 10:48:44 2001



From: David_R_Stadden-at-armstrong.com
Date: Mon, 2 Jul 2001 11:46:39 -0400
Subject: SEM Service Life?

Contents Retrieved from Microscopy Listserver Archives
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I have cross sectioned this sample along the axis of the wires and see
interesting features.

The defect terminal was determined to contain 13% Cr (EDS standardless
analysis of the Cross Section), whereas the other materials were determined
to contain 21% Cr. I checked with my client and he had chosen to use a
lower grade material for the defect terminal, because it had the same high
temperature ratings. I was able to peel back the cross sectioned element
(21%Cr material) and examine its surface as well as the surface of the hole
wall (13%Cr terminal material). The hole wall was completely covered by a
binary particulate material. The particles contained either Ti and oxygen
or Al and oxygen. The element material was covered approximately 70% with
this material. Examination of the cross section showed this layer, but also
a particle field that was evenly dispersed through the defect terminal and
ended a few hundred microns past the weld interface between the terminal and
the element. These particles have a lower Z than the matrix and are on the
order of 1 micron. Elemental analysis of the particles embedded in the
terminal material shows mostly Ti, C, and N, with lower levels of Zr.

My proposed mechanism for the failure is that either the media in the
furnace or the refractory material of the furnace has corroded the surface
of both the "A-1" grade element and the lower grade terminal material. The
lower grade terminal material has also had a migration of the surface
corrosion into it internal structure and into the vicinity of the weld.
This internal corrosion is what eventually gives rise to the electrical
failure. The flowed material, that was initially investigated, I believe to
be a "symptom rather than a cause of the failure (as Woody White had
stated)".

My questions now are: Is this a plausible mechanism?

Can the corrosive particulate, on the order of 1 micron, migrate 100's of
microns into a low grade ferrous alloy at temperatures of 1100°C? (Although
localized temperature could reach higher levels)

I have images if anybody would like to see the features I discuss.


Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756


-----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
Sent: Wednesday, June 27, 2001 1:33 PM
To: To all on the list




I'm curious about whether there's any published data on the average lifespan of
scanning electron microscopes in an industrial environment. Our first SEM we
had for 15 years, now we're up to 13 on the current system. My manager, being
the sort of histogram-oriented guy he is, asked me for some longevity data. In
the absence of any previously published data, I'd sure be interested in
individual experiences along these lines.

Much obliged,

Dave Stadden
Research Scientist, Testing and Analysis Lab
Armstrong World Industries, Inc.




From daemon Mon Jul 2 11:19:03 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 2 Jul 2001 12:10:27 -0400
Subject: RE: UA ka ka on grids

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Dear Eric,
You said nothing about the solvent.??!!!? While switching out a
filter on the building supply, did someone make an error? Etc.

You didn't mention the morphology of the ka ka. Smaller than .45um?

Are these the clumps of homogeneously very small crystals/grains/?
that often appear in older mixes/solutions?

Has someone else taken sample from the bottle recently?

Do you do your staining on an open bench? In a hood? In a Petrie
dish?

Try .22um filter. If still present, then how old is jar of U salt?

Some other things to think about:

Biological processes involved with U depletion/removal in
environment may have applicability to lab situations. See:
(http://www.nttc.edu/env/Uranium/Uranium_chap8.html). Almost ALL UAc(Uranyl
acetate) has some trace of Fe in it - increasing the possibility of a slowly
developing linked biological process leading to production of "increasing"
amounts of UO (fine grain ppt?) in the reservoir of UAc. DID you know that
uranium (U) is 'pyrophoric'?
Then there is all that acetate! See:
(http://www.geol.vt.edu/research/gssrs/gssrs2000/abstracts/tkendall.html).

What's different about the specimen, or is this happening
with ALL specimens?

[With regard to your concern about your concern. No, you should
never become complacent. Such aggravations must be removed. You must
cultivate your crisis management skills. If you don't, you will feel guilty
and inadequate. It would be better to manifest OCD than to feel 'guilt and
'inadequacy.']


Regards and best wishes,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: Eric
} Sent: Friday, June 29, 2001 6:24 PM
} To: 'Microscopy Listserver'
} Subject: UA ka ka on grids
} Importance: High
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there fellow microscopists,
}
} Here in the Pathology EM lab we do not do rocket science, but standard
} thin-section TEM of renal biopsies.
}
} In the last two weeks though I have been getting some ka ka (technical
} term) on my grids from my UA stain. I have already made up two new
} batches
} of UA stain and use .45 micron filters just before use. Question is that
} all of the sudden I am getting UA stain artifact on my grids. I have not
} have any trouble since I have been here.
} I have changed everything, new staining plate, cleaned and scrubbed
} everything that I use for staining. I still get some UA artifact. Is
} this
} normal and should I just forget about it or is there something else wrong
} I
} am missing now. I mix up a 2% UA stain of 50% methanol and 50% water. I
} stain for usually 10-12 minutes. the grids before staining or clean, but
} after UA stain they are a bit dirty...
}
} Any suggestions?
}
} Thanks,
}
} Eric A. Rosen
} UCLA Medical Center
} Dept. Pathology
} EM Lab
}
}


From daemon Mon Jul 2 11:34:06 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 2 Jul 2001 12:28:06 -0400
Subject: RE: counterstaining for immunofluorescence

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"Hypericin" - the active principal in St. John's Wort, a quencher? See:
http://www.phys.uni.torun.pl/~lum98/p_abstr/hovhanis.html

It apparently absorbs strongly in the yellow!! See:
(http://www.aspjournal.com/public/vol71/iss2/abstracts/abstractvol71iss2pp18
8-194.htm)

Might be worth a look.

In any case, the principle of quenching autofluorescence should be clear to
those wondering what's going on.

Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Shea Miller
} Sent: Friday, June 29, 2001 11:51 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: counterstaining for immunofluorescence
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi again;
} we are having a wee bit of trouble distinguishing our signal from the
} autofluorescence in our immunoassay on immature soybean seeds/seed coats.
} So far, we have used a light stain of toluidine blue, but I'm not
} completely happy, and am considering Evans Blue instead. What do others
} use to quench the yellow autofluorescence (our Ab is FITC labelled) in
} plant tissues, and when do you actually apply the counterstain? Before
} the Ab incubations, or after??
}
} thanks so much again in advance
} shea
}
}
}
} Dr. S. Shea Miller
} Agriculture & Agri-food Canada
} Eastern Cereal and Oilseed Research Centre
} Central Experimental Farm
} Ottawa, Ontario
} Canada K1A 0C6
} Phone: (613) 759-1760
} Fax: (613) 759-1701
} Email: millers-at-em.agr.ca
}
}
}


From daemon Mon Jul 2 12:20:26 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 2 Jul 2001 12:05:28 -0500
Subject: RE: Part II, proposed mechanism...

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Ric,

Food for thought:

While I cannot remember ever seeing migration of particulate oxide into a
metallic, it is certainly possible for oxygen to diffuse into the material
and (then) form oxides.

I have not checked references for the materials in use, but typically, the
alloys with lower Cr concentrations are more likely to oxidize/corrode.
Again, I need to check the references to be exact, but chromium oxide has a
fairly low vapor pressure at 1100 C. Not only can diffusion occur at this
temperature, but you may also vaporize cr that has oxidized and re-deposit
nearby on cooler surfaces.

As a matter of fact, the first pix I saw of the oxide crystals looked more
like a vapor deposition structure than a product of surface corrosion.

Another thought... As the electrical joint oxidizes and begins to fail, the
resistance likely rises. For the same power input, this would result in
localized heating which could go far higher than the 1100 C process
temperature. Higher temps would equal faster oxidation / diffusion, further
raising the resistance. The worse it gets, the worse it gets...

Hard to accurately evaluate all variables long distance, but it sounds like
you are making progress and narrowing the possibilities. I am still
concerned about any lube residue which could break down at temp and liberate
elements (hydrogen, oxygen, halogens, sulfur, etc.) which could accelerate
corrosion for many materials.

Woody White
McDermott Technology, Inc.


From daemon Mon Jul 2 12:54:25 2001



From: Dr. Edgar Voelkl :      mm2002-at-ornl.gov
Date: Mon, 02 Jul 2001 13:48:56 -0400
Subject: M&M 2002 in Quebec

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Dear fellow microscopists,

I would like to thank all of you who have responded to the
solicitation for suggestions for symposia for the M&M annual meeting
in 2002 in Quebec. The response was very strong and it will be
plain impossible to accommodate all suggestions. I thought it
worthwhile to mention that the biological sciences area has received
far more suggestions than the physical sciences area. Is that a sign?

Anyway, I want to very much thank all of you for the suggestions and
at the same time ask for your forgiveness that I will not be able to
respond properly to all who have put up the effort to write. If you
will not hear from me over the next few weeks, please be assured that
your suggestions have at least contributed to emphasize certain
directions for the symposia.

It is great to see how active our society is.

Best regards,

Edgar Voelkl
Program Chair M&M 2002

--

___________________________

Dr. Edgar Voelkl
Program Chair M&M 2002

Oak Ridge National Laboratory
P.O. Box 2008
Bldg 4515
Oak Ridge, TN 37830-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913


From daemon Mon Jul 2 12:55:44 2001



From: Bradley Starcevich :      microscopist-at-excite.com
Date: Mon, 2 Jul 2001 10:51:06 -0700 (PDT)
Subject: TEM

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Greetings,

I have a Zeiss EM-9S-2 TEM for sale. Needs vacuum pump
and chiller. Unit is in excellent condition. Have all manuals and many
miscellaneous spare parts.
Best offer. Buyer responsible for packaging and freight.

Contact:

Bradley K. Starcevich
microscopist-at-excite.com






_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon Jul 2 12:57:31 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 2 Jul 2001 13:48:47 -0400
Subject: Re: UA ka ka on grids

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At 3:24 PM -0700 6/29/01, Eric wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

******************
I know this sounds like a cop-out (am I dating myself with that
phrase, or what?), but try a new bottle of UA. I had the same
problem years ago, and that's what I finally resorted to. It was
easier and more comfortable than continuing to knock my head against
the wall!
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Jul 2 13:14:53 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 02 Jul 2001 12:42:22 -0500
Subject: Re: SEM Service Life?

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I think the issue is probably determined by when support gets hard to find
or when the accumulation of new features reaches a critical level.

We have a JEOL 840A that is about 15 years old and is still doing fine. It
does not offer the integrated control interface to our EDS system like
newer units might. But the imaging is still good and JEOL is still
supporting it. I can easily imagine it going another 5 years or more.

I guess I would toss out 15-20 years as an expected life. Labs that insist
on having the latest and greatest might turn a scope around in less than
ten years.

That's one man's opinion (whose newest car happens to be a 1989).
Warren

At 11:46 AM 7/2/2001 -0400, you wrote:

} I'm curious about whether there's any published data on the average
} lifespan of
} scanning electron microscopes in an industrial environment. Our first SEM we
} had for 15 years, now we're up to 13 on the current system. My manager, being
} the sort of histogram-oriented guy he is, asked me for some longevity
} data. In
} the absence of any previously published data, I'd sure be interested in
} individual experiences along these lines.
}
} Much obliged,
}
} Dave Stadden
} Research Scientist, Testing and Analysis Lab
} Armstrong World Industries, Inc.



From daemon Mon Jul 2 15:05:46 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 2 Jul 2001 16:02:10 -0400
Subject: Glycol in chilers

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In a recent discussion of how to avoid algal growth in water
chillers, someone suggested using a rather high concentration of
ethylene glycol. I have serious reservations about employing this
method. Ethylene glycol is a hygroscopic substance, and fairly high
concentrations of it are required to be effective. If for any reason
(and water lines have been known to leak on numerous occasions and
for a variety of reasons) some of this solution gets into any part of
an instrument (and I have seen cooling water get onto circuit boards
and into various internal parts of instruments on several occasions),
it can be very difficult to remove. Worst of all, because of its
hygroscopic character, any residue that is left can pick up water
from the atmosphere, keeping that part of the instrument moist
forever after. The control of algal growth in electron microscopes
is discussed in more detail on page 216 of my book, 'Vacuum Methods
in Electron Microscopy (for a description see
http://2spi.com/catalog/books/book48.html and
http://pup.princeton.edu/titles/6484.html)
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Mon Jul 2 15:09:26 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 02 Jul 2001 16:04:42 -0400
Subject: Confocal

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Dear Jon,
We at the University of Kentucky also have a core facility that originally
was just EM. We have also experienced a slow down in TEM use compared to
years past. In the last three years we have added 2 confocals. We have an
upright and an inverted, both Leicas. Most of our users are not in our
building but no one seems to complain about the hike. They just cover
their samples with foil and make the trek.
We expect everyone to supply their own antibodies and we supply training,
help and advice (for a price of course). We have sign-up sheets and on
busy days limit users to 2 hour blocks of time. Having two instruments
does help if someone needs to do a time lapse study.
Users constantly have questions or problems that need to be resolved, so
it's important that someone in the lab be quite familiar with the
instrument and it's software. For the users who prove to be proficient, we
allow after hours use. Otherwise someone is always around . The group
whose money purchased one of the instruments pays the same as everyone
else. The biggest problem we've had is making people wait when the
instruments are down. Service has been very slow.
Let me know if we can help you in any way and good luck.

Mary Gail Engle, manager
Electron Microscopy & Imaging Facility
University of Kentucky
Lexington, KY


From daemon Mon Jul 2 15:32:36 2001



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 02 Jul 2001 15:25:24 -0500
Subject: Bulb shock?

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I have a Zeiss OPMI surgical microscope. I bought it used as-is.
It came with a bulb that didn't last. The filament broke away
from the support wires. A fine spiral of thinner wire wound around
the support wires seemingly to help support the filament, and it
had spiralled off the supports.

Fortunately my junk pile revealed a replacement bulb. However,
after the 'scope took a trip in the car to demo for some
kindergarteners, its filament also broke and the thin wires
spiralled away.

$45 at Bulbdirect.com and I'm back to a working bulb and a spare.
What should I be doing to protect myself against early lamp
failure? Cool-down without movement for 20 minutes? Remove
lamps before driving around with the 'scope? Are short bursts
of usage (5-10 minutes each) to blame?

- John

P.S. Dr. Monson: Love the stories. Keep in mind that all
seemingly newbies on the 'net might be learning very slowly
over time, just for fun, and without benefit of a degree program.
The Web is our Oracle, but I'll try to pay attention during class.
Please do not hit me with eraser, chalk or ruler.



From daemon Mon Jul 2 16:14:14 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 2 Jul 2001 17:07:48 -0400
Subject: RE: scintillation crystals

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Hi Sterling,

What a coincidence! The same question came up here and I entered "511
photon" and asked "Google.COM".

I immediately received for example:
http://cfi.lbl.gov/instrumentation/Pubs/4on1.pdf

That seemed to satisfy my questioner, so I quit. I hope someone who really
knows the field is listening.

Fred Monson

} ----------
} From: sterling stoudenmire
} Sent: Saturday, June 30, 2001 11:26 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: scintillation crystals
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} i am trying to find a way into the literature on experimental techniques
} appropriate to study 511 photon detection and capture vs properties of
} scintillating materials. Is there a standard way to do this?
}
} Can someone point me at a good place to begin a literature survey?'
} sorry, my question is not better phrased, but I am just beginning to look
} at this?
}
} sterling
}
}
} Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find
} the cure for cancer and other diseases. There will always be a need for
} the trained clinician (MD/RN) but, advanced diagnostic and treatment
} option
} selection has become gene based, has moved from the physician's practice
} to
} the computerized cell and molecular biology laboratory, and appropriate
} treatment options should now be based on the personal biology of the
} patient.
}
}


From daemon Mon Jul 2 17:24:41 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 02 Jul 2001 18:21:49 -0400
Subject: Re: SEM Service Life?

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Dave,
It really depends upon how the system is cared for. I'm still servicing
microscopes that date back to 71 that work just fine. They were 6 years
old when I STARTED servicing them. Red Lion High School (just across
the river from you) has an SEM that's older than at least one of the
teachers who uses it (and all of the students).

A more germain question would be "Is the current microscope able to do
the things we require, and do them well and efficiently?" If the answer
to this is "yes" and you have adequate service to keep the system
running, there is probably no reason to even think about replacing it.

On the other hand, if newer technologies could improve your performance
significantly, then you might want to consider upgrading. The
downside? When the PC that is running the latest system becomes
obsolete (3 years? 5 years?) and the rest of your system (vacuum, High
Voltage, etc.) is still fine, what are you going to do? Try to find a
new 486 with Win 3.1 to replace a crashed but fairly recent Amray, for
example. Their boards and software won't run on anything newer. I'm
not picking on them, I'm just familiar with them.

Alternatively, EDS systems have a notoriously short lifespan and it's
getting shorter. Can the addition of a beam control interface and a new
EDS system give you all the new stuff you need (or want)? This tends to
be less expensive and easier to justify because of the historic turnover
rate. The bonus being that all users are at least familiar with the
basic SEM and don't have to start from ground zero (if you have a lot of
users, this could be a consideration). This is an easy way to get
digital imaging, for example.

Just my $.02. You should get a lot of interesting and useful replies.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA (York County)






"David_R_Stadden-at-armstrong.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} I'm curious about whether there's any published data on the average lifespan of
} scanning electron microscopes in an industrial environment. Our first SEM we
} had for 15 years, now we're up to 13 on the current system. My manager, being
} the sort of histogram-oriented guy he is, asked me for some longevity data. In
} the absence of any previously published data, I'd sure be interested in
} individual experiences along these lines.
}
} Much obliged,
}
} Dave Stadden
} Research Scientist, Testing and Analysis Lab
} Armstrong World Industries, Inc.
}
}
}
}
}



From daemon Mon Jul 2 18:22:22 2001



From: Eric :      biology-at-ucla.edu
Date: Mon, 2 Jul 2001 18:18:17 -0500
Subject: Re: UA ka ka on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:02 AM 7/2/01 -0700, Markus F. Meyenhofer wrote:
} I am using a 50% Methanol and 50% ddH20 as my solvent for the solution of
} UA stain.
}
} the particles are not fine grain, bit somewhat larger in size.. I have not
} measured them as of yet, but will try to do that today...
}
} The solutions are relatively fresh.. I just made them last Monday...
}
} No one else uses this solution.. I mix it for myself to use...
}
} Staining is done on a clean petri dish with a dental wax base.. and
} covered.. the grids are submerged in the droplet for 12 minutes..
} I had been doing 15 minute staining till I mixed up this batch of stain and
} had to cut back on the time...
}
} I have on order some .2um filters..
}
} 5 minute staining is too light.. and still has some schmutz on the grid...
}
} We have been using for the past two years a sterile water we buy in 1500ml
} bottles. and have never had a problem till recently...
}
} I use a small set of three beakers that are filled with water and the grids
} are dunked in each beaker 10 times then put on a clean piece of filter paper
} ...
}
} We, use a modified karnofsky fixative... no phosphate


From daemon Mon Jul 2 18:55:40 2001



From: MSAMCC-at-sparc5.microscopy.com
Date: Mon, 2 Jul 2001 19:06:07 -0500
Subject: Microscopy Society of America (MSA) Needs You

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The lifetime for a given SEM depends greatly on the support from the
manufacturer.
As Warren has pointed out, the JEOL 840A has a life expectancy of at least
another 5 to 10 years.
JEOL has a policy of supporting their equipment in excess of twenty years.
They use older machines that have been "traded-in" to support others when
parts are unavailable from the factory. I can still remember having parts
available for the JEOL U3 which is a tube model.

On the other side of the coin Amray & Topcon have since not supported the
older SEMs and the SEM value and longevity has diminished.
At present I have an Amray 2030C that I am considering using for parts. It
is ashamed to do this as the instrument in only six years old & costs over
350K new.

My car is a 1992 Honda.

Regards,

Earl




----- Original Message -----
} From: "Warren E Straszheim" {wesaia-at-iastate.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Cc: {David_R_Stadden-at-armstrong.com}
Sent: Monday, July 02, 2001 10:42 AM



} From: Microscopy Society of America: Marketing and Communications Committee
(MSAMCC)

Colleagues:

MSA is a vibrant society which encompasses all facets of Microscopy
and Microanalysis. The society and it's members are dedicated to the
promotion and advancement of the knowledge of the science and practice
of all microscopical imaging, analysis and diffraction techniques useful
for elucidating the ultrastructure and function of materials in diverse
areas of biological, materials, medical and physical sciences. To this end
MSA is a resource which is second to none throughout the world. If you're
not currently a member of MSA, then you're missing out on both the
opportunities and the fun; and we would like to take this opportunity to
invite you to join our family.


If you are already an MSA member, take a moment to talk to your colleagues
and/or students about MSA's great benefits and encourage them to join the
society. Invite them to be come part of our world wide microscopy community.


MSA Membership Benefits Include:

* A subscription to the high-quality journal: Microscopy & Microanalysis
-included with your membership dues
http://www.msa.microscopy.com/MM/MscopyManalysis.html
* Receive advanced information and reduced fees for the Annual Meeting
- the preeminent microscopy related meeting and exhibition
http://www.microscopy.com/MSAMeetings/MMMeeting.html
* Access to extensive educational materials & activities
http://www.msa.microscopy.com/RefEdu.html
* On-line Directory of MSA members
http://www.msa.microscopy.com/MSAMembers/SearchMembersDB.html
* Opportunities to have your accomplishments recognized as an Awards recipient.
http://www.msa.microscopy.com/MSADocs/MSAAwards.html
* MSA Placement Office
http://www.msa.microscopy.com/PlacementOffice/JobListings.html
* MSA Certification Program
http://www.cvmbs.colostate.edu/emcenter/msa/certboard/
* MSA Technologist Forum
http://www.cvmbs.colostate.edu/emcenter/msa/techforum/
* MSA Public Policy Committee
-remain aware and influence governmental funding opportunities
http://www.msa.microscopy.com/PublicPolicy/
* MSA Sustaining Members Program
http://www.msa.microscopy.com/cgi-bin/SMDBListing.pl
* MSA Vendor News and Surplus Equipment Listings
http://www.msa.microscopy.com/News/NewsListings.html
http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html
* Reduced prices for books and journals
* Subscription to the MSA Bulletin to keep abreast of Society activities
* Specialized networking via participation in "Focussed Interest Groups" with
microscopist's having similar interests to yours
* and of course the International Networking facilitated in part by
the Microscopy
Listserver to keep abreast of worldwide microscopy activities
and information.

So if your not a member of MSA, take a moment and join!
It is easy and inexpensive [$15/Students, $ 45/Regular,
$63/outside of North America and don't forget this includes the
a subscription to the Journal].

You can become a Member On-Line at
http://www.msa.microscopy.com/MSADocs/MSANewForm.html
or request that an printed application form be sent to you by
sending an Email to:
MSABusinessOffice-at-msa.microscopy.com


Remember MSA needs YOU !
--------------------

MSA Marketing and Communications Committee
MSAMCC-at-msa.microscopy.com


From daemon Mon Jul 2 19:54:03 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 3 Jul 2001 10:48:53 +1000
Subject: RE: Microscope magnification, stage micrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Agreed, a stage micrometer is the best way, but they are not that expensive. As
always, shop around, but I expect that you will find the microscopy supply
businesses offer the better deal. We list five such micrometers and all but one
cost less than US$150 including freight.
Disclaimer: ProSciTech sell stage micrometers and so has an obvious interest
Cheers
Jim Darley
ProSciTech

On Tuesday, July 03, 2001 1:10 AM, Debby Sherman [SMTP:dsherman-at-purdue.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Roy,
} Invest in a good micrometer slide and use it to determine magnification.
It
} may seem expensive initially but, since it can be used on any coumpound
} microscope regardless of set-up and camera, it will pay for itself in
} convenience (and accuracy of determining magnifications) for years to
come.
} You can purchase them through your microscope venders for both compound
and
} stereo microscopes.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
}
} S-099e Whistler Building
} West Lafayette, IN 47907
}
} --------------------------------------
} Date: Friday, June 29, 2001 4:16 PM
} } From: Beavers, Roy {rbeavers-at-post.cis.smu.edu}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Need some help trying to figure the magnification of some photos taken with
} the following:
}
} Nikon Optiphot-Pol Microscope
} CF eyepiece 10X
} CF objective 20X
}
} with Nikon AFX-II camera control assembly using a Polaroid 4x5 film holder
} with a 4X marked on it.
}
} Focus for images was done through the monocular eyepiece on the camera
} assembly.
}
} I assume that my magnification though the microscope eyepiece would be 30X
} I assume that the magnification to the camera back would be 24X
}
} Sorry if this seems like a silly question but I do not do much optical
} microscopy and got to thinking that it may be more than just the sum of the
} optics magnification.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu
}
}



From daemon Tue Jul 3 02:46:18 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 03 Jul 2001 17:37:34 +1000
Subject: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Somebody just asked me the reference for toluidine blue/sodium borate (1% each) which is our routine LM stain - I cant find an attribution in any of about 10 books. Can anybody help? This person should not be forgotten! One suggestion was Alsop?

thanks

Sally



Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU





From daemon Tue Jul 3 03:56:42 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 3 Jul 2001 18:53:17 +1000
Subject: Re: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Sally,

In an old (1976) book on botanical microtechnique, there is a reference for
1% tol blue in 1% borax - Trump BF, Smuckler EA, Benditt EP (1961) A method
for staining epoxy sections for light microscopy. J Ultrastr Res 5:343-348.


There's also Mallory's borax methylene blue, maybe tol blue was a
substitute for the methylene blue?

cheers,
Rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Jul 3 06:20:42 2001



From: Bertaska, Richard :      BertaskaR-at-corning.com
Date: Tue, 03 Jul 2001 07:07:22 -0400
Subject: SEM Service Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SEM service life is based on parts availability and support.

Many older SEMs are still supported because the failing parts/components are
still manufactured. Fortunately manufacturers used many readily available
parts. Also many institutions/businesses have purchased new equipment and
"retired the old". Fortunately there are some enterprising individuals who
recognized a niche and began collecting "abandoned" SEMs. These individuals
now provide a necessary parts/service to SEM industry.

As for support....if you can do much of your own you are smart. For those of
us who neither have the time or expertise we need to seek out the
experts/entrepreneurs.

I currently manage three SEMs. The oldest is AMRAY1600 purchased in 1984.
It is a workhorse for us. Despite minimal maintenance and no service
contracts over the past years it has performed well. Recently a number of
components began to fail. It was suggested that the system be retired and
replaced.

I found expertise in SEMTech Solutions Inc. During the past year we have
replaced several aging components, added digital imaging, and moved the SEM
to another building for access to multiple users. EDS upgrade is also being
considered. As long as parts are available I expect the system to perform
another 20 years!

SEMTech provided expertise and parts. I would highly recommend them for
AMRAY instrument service.

c/o Jim Peterson
PO Box 2155
Natick MA 01760
tel: (978) 663-9822


I have no vested interest in the company. I am simply a truly satisfied
customer with an excellent service provider.

Finding other experts for other SEM instrumentation may be difficult.
However, if the SEM community needs/supports it, I think there are
individuals prepared to provide it.

Perhaps the reason these smaller new EDS ventures have been able to
establish themselves is because the larger established businesses no longer
listened/responded to their customer needs. A similar circumstance could
evolve with SEM maintenance.


The question poses another thought: How could the SEM community "inventory"
all SEMs by brand, model, age, location, condition, and status. Thus all
SEMs being accounted for could effectively provide the potential inventory
parts list and availability.





From daemon Tue Jul 3 07:33:15 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 3 Jul 2001 08:27:11 -0400 (EDT)
Subject: Double-tilt holder wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear everybody,

I have been seeking a double-tilt holder for our 2000fx scope for some
time with no success. This holder is to be used for general teaching in
the up coming semester. Purchasing a new holder takes a bit too long to
be delivered. Also thought 2000fx holders are compatible with those of
JEOL100 type scopes. So I may have a better chance to acquire a used one.
Anyway, if you have one sitting in your lab with no immediate use, please
contact me off line. Thanks much. Have a great holiday!

*********************************
Chaoying Ni
Electron Microscopy Laboratory
Materials Science and Engineering
College of Engineering
University of Delaware
Newark, DE 19716
*********************************



From daemon Tue Jul 3 08:11:44 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Tue, 3 Jul 2001 09:05:30 -0400
Subject: Re: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

As the image is in a SEM is created by lines of information, many problems
exhibit themselves by giving saw tooth edges to the lines. The slower you
scan the better the instrument is at synchronizing out the interference.

The most common reasons for the above are:-

1. Vibration
2. External magnetic fields

If we consider operating parameters we may be able to track this down.
Magnetic fields will have a greater affect at lower accelerating voltages
and at longer working distances, in both cases the final lens strength is
reduced allowing the external field to overcome what protection the lens
field provides. Try working at the shortest possible working distance and
at the highest kV, probably about 5mm at 30kV. If this reduces or removes
the problem it would be an external field that is the culprit. Another
indication of an external field problem is the TV rate scan will have a sub
ripple that will work its way down the screen.

We need to know if you have been able to take good photographs above 5,000X
in the past. If so we then can assume the problem is a result of a change
in the laboratory; a new piece of equipment is giving off the field. This
may be many feet away, the field effect is according to the inverse square
law. Try going round switching off new equipment and see if that helps. I
have seen problems due to incorrect wiring of new equipment so if you find
a field source have a technician check the wiring before going any further.

Please come back if you need more help.

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Tue Jul 3 08:47:40 2001



From: Viana de la Iglesia, Felix :      felix.viana-at-umh.es
Date: Tue, 3 Jul 2001 15:39:11 +0200
Subject: need protocols for confocal detection of immunostaining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listmembers:

I would like to perform a series of immunostains on cultured neurons to
detect membrane proteins using confocal microscopy.
The animal used is mouse and the antibodies were generated in rabbit.
I plan to use a biotinylated secondary antibody and an avidin/streptavidin
conjugated fluorochome that can be excited by the argon (488 nm) or HeNe
(543 nm) laser lines of a confocal microscope.
I need information on the source of these fluorescence compounds and your
experience with Alexa/Cy dyes for this type of work.

Thank´s for your help.

Félix Viana


=============================================================
Dr. Felix Viana
Instituto de Neurociencias
Universidad Miguel Hernandez / CSIC
Apartado 18
San Juan de Alicante
03550 Alicante (SPAIN)

phone(s): 34-96-591 9347 (9367)
fax: 34-96-591 9547
e-mail: felix.viana-at-umh.es






From daemon Tue Jul 3 09:48:40 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Tue, 03 Jul 2001 10:39:01 -0400
Subject: IEM for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists,

We are doing an IEM for SEM with bacteria and would appreciate any
comments. We are looking for surface label 5nm gold with a FIESEM. Our
protocol is:

3% PF 0.05% GA fix PO4 buffer

rinse

Bock in 1%NGS

rinse

Primary ABY incubation O/N cold

rinse

Secondary gold conjucated ABY 1hr

rinse

Hard fix 2% GA same buffer

rinse

2% UA filtered DH2O

Dehydration

Critical Point Drying

Our questions are is the UA enough to stablize the lipds throughout the
dehydration and cpd? We believe osmium would strip off the gold and
tannic acid may make small precipitates similar in size to the gold.
We will use in-lens and backscatter to view the gold. Any other
suggesstions would be appreciated.

Thank you

Mike D



From daemon Tue Jul 3 09:48:42 2001



From: GIDDINGS THOMAS H :      giddings-at-spot.colorado.edu
Date: Tue, 3 Jul 2001 08:43:29 -0600 (MDT)
Subject: Job Opening-PRA-Boulder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The position described below was recently announced at the University of
Colorado. Those interested in applying should contact Dr. Andrew
Staehelin directly.

Tom Giddings

Thomas H. Giddings Tel: (303) 492-8402
MCDB Microscopy Facility Fax: (303) 492-7744
University of Colorado giddings-at-spot.colorado.edu
Boulder, CO 80309-0347

} From: Andrew Staehelin {staeheli-at-spot.Colorado.EDU}

OPENING FOR PROFESSIONAL RESEARCH ASSISTANT

I have a job opening for a Professional Research Assistant starting
sometime in August.

The research is on structure function relationships of the Golgi apparatus
in yeast (Pichia pastoris) and plant cells. The work will involve cell
culturing, the growing of Arabidopsis plants, light microscopy studies of
cells expressing GFP constructs, electron microscopy of high pressure
frozen cells, EM tomography and immunolabeling experiments. The successful
candidate will also be involved in some lab administration duties.

Starting salary is in the $24,000 to $27,000 range, depending on experience.

Please send application, including curriculum vitae and names of three
references, to Andrew Staehelin, MCD Biology, UCB 347, University of
Colorado, Boulder, CO 80309-0347, or email to: staeheli-at-spot.colorado.edu.
Application deadline: July 15, or until position is filled.

Andrew Staehelin
MCD Biology
University of Colorado
Boulder, CO 80309-0347
U.S.A.

Tel. +1-303-492-8843
Fax. +1-303-492-7744
E-mail: staeheli-at-spot.colorado.edu



From daemon Tue Jul 3 10:02:06 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 3 Jul 2001 10:53:44 -0400
Subject: Re: Confocal questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jon,

Gee, I wonder how many of us wear the same, multiple hats? I have
been the director of the EM facility here for 13 years, and for the
past 4 have also managed the optical microscopy core which consists
of a confocal and a widefield fluorescence. We hope to add a second
confocal in the next year or so.
Our users come from various points in the med. school complex and we
have some that come from other institutions. Travelling with
prepared, fixed samples is not a big deal. Its only the folks who
need to look at live cells that have a problem.
Our confocal was purchased with an NIH SIG, but the main PI on that
grant, who is also the Chair of the dept. in which it resides, pays
the same fees as everyone else. We limit each user to 2 blocks of up
to 3 hours each per week. People who have been fully trained and
"certified" by me are allowed access 24/7. (Our fees drop to half
after hours to encourage them!)
If you have the space in your lab, go for it. My EM facility is in
one dept. at one end of the complex & the optical 'scopes are in
another dept at the opposite end. I can log a mile a day just
walking between the 2 facilities on days when the confocal or its
users are acting up.

Our users are responsible for fully prepping their own samples. We
supply immersion oil & lens paper. As a courtesy, we stock CD-Rs and
bill for what they cost us. In additon to the microsocpes, we have 2
computers ( a PC & a MAC G4) for data analysis. This keeps the
microscopes available for data acquisition.

Coming into confocal as a way to save my position a few years ago, I
have only worked with a very old Bio-Rad, which was very reliable,
and great for its vintage, and our current Zeiss LSM-510.
considering how complex the software must be to do all the things it
does, I am always amazed that any of the confocals work. As with any
software-driven equipment, you have to expect a few glitches now &
again but overall, we have been quite pleased. I can usually get my
users up & running fairly independently after about 6-8 hours of
training.

Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jul 3 10:58:59 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 3 Jul 2001 11:48:54 -0400 (EDT)
Subject: RE: UA ka ka on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After checking the obvious (filters, water, etc.) try diluting the stain
slightly--5-10 % with water. We have had success with this. I suspect
the UA (in some cases, at least in ours) may be precipitating out. Also
keep the staining dish covered to prevent evaporation (and precipitation).
Finally, make sure the drop clinging to the inside of the forceps is drained
from behind to prevent it's contaminating the grid.

Sara



On Mon, 2 Jul 2001, Monson, Frederick C. wrote:

} Date: Mon, 2 Jul 2001 12:10:27 -0400
} From: Monson, Frederick C. {fmonson-at-wcupa.edu}
} To: 'Eric' {biology-at-ucla.edu}
} Cc: 'Microscopy Listserver' {microscopy-at-sparc5.microscopy.com}
} Subject: RE: UA ka ka on grids
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Eric,
} You said nothing about the solvent.??!!!? While switching out a
} filter on the building supply, did someone make an error? Etc.
}
} You didn't mention the morphology of the ka ka. Smaller than .45um?
}
} Are these the clumps of homogeneously very small crystals/grains/?
} that often appear in older mixes/solutions?
}
} Has someone else taken sample from the bottle recently?
}
} Do you do your staining on an open bench? In a hood? In a Petrie
} dish?
}
} Try .22um filter. If still present, then how old is jar of U salt?
}
} Some other things to think about:
}
} Biological processes involved with U depletion/removal in
} environment may have applicability to lab situations. See:
} (http://www.nttc.edu/env/Uranium/Uranium_chap8.html). Almost ALL UAc(Uranyl
} acetate) has some trace of Fe in it - increasing the possibility of a slowly
} developing linked biological process leading to production of "increasing"
} amounts of UO (fine grain ppt?) in the reservoir of UAc. DID you know that
} uranium (U) is 'pyrophoric'?
} Then there is all that acetate! See:
} (http://www.geol.vt.edu/research/gssrs/gssrs2000/abstracts/tkendall.html).
}
} What's different about the specimen, or is this happening
} with ALL specimens?
}
} [With regard to your concern about your concern. No, you should
} never become complacent. Such aggravations must be removed. You must
} cultivate your crisis management skills. If you don't, you will feel guilty
} and inadequate. It would be better to manifest OCD than to feel 'guilt and
} 'inadequacy.']
}
}
} Regards and best wishes,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}
}
} } ----------
} } From: Eric
} } Sent: Friday, June 29, 2001 6:24 PM
} } To: 'Microscopy Listserver'
} } Subject: UA ka ka on grids
} } Importance: High
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi there fellow microscopists,
} }
} } Here in the Pathology EM lab we do not do rocket science, but standard
} } thin-section TEM of renal biopsies.
} }
} } In the last two weeks though I have been getting some ka ka (technical
} } term) on my grids from my UA stain. I have already made up two new
} } batches
} } of UA stain and use .45 micron filters just before use. Question is that
} } all of the sudden I am getting UA stain artifact on my grids. I have not
} } have any trouble since I have been here.
} } I have changed everything, new staining plate, cleaned and scrubbed
} } everything that I use for staining. I still get some UA artifact. Is
} } this
} } normal and should I just forget about it or is there something else wrong
} } I
} } am missing now. I mix up a 2% UA stain of 50% methanol and 50% water. I
} } stain for usually 10-12 minutes. the grids before staining or clean, but
} } after UA stain they are a bit dirty...
} }
} } Any suggestions?
} }
} } Thanks,
} }
} } Eric A. Rosen
} } UCLA Medical Center
} } Dept. Pathology
} } EM Lab
} }
} }
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Jul 3 11:07:14 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 3 Jul 2001 12:02:37 -0400
Subject: RE: DPX versus Canada Balsam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Morning Micha,
First, I can only provide my experience on this matter.
Second, I will summarize what I have trundled on about below for the
purpose of those who are interested in the destination, not the journey.

0. Yes, I have seen "Permount" preps of this age, so
I'm certain that mountant, properly applied, will work. The slides I made
as a student in 1964 with many different dyes and stains - mountant Gum
Damar - are still as 'perfect' as my "B" illustrated.
1. With respect to vapor pressure:
Xylene {Toluene {Benzene
2. Three problems with mountants:
a. Resin concentration must be close to
saturation to minimize evaporative changes in volume after application.
b. Mountant volume under cover glass must be
close to minimum (I standardize this with weights after application!)
c. Resin composition must NOT cause
'unacceptable' changes in specimen (e.g., acidity?).

For years, I have used a natural resin called Gum Damar (or Dammar!)
(apparently 'mined' from the same quarter of the planet as hematoxylin),
solubilized in Xylene (Xylene {Toluene {Benzene(vapor pressure). This
preparation has failed for only one reason that I can discern - too much
solvent in the drop(s) used to seal the cover glass. The major problem is
loss of solvent via evaporation.

Most of the mountants that are commercially available are
solubilized in Toluene, thus, they tend to 'dry' somewhat faster than the
prep I have used. While the commercial preparations tend to dry faster,
they do not appear to suffer from the other problem apparent in natural
preps like Damar that is acidification and/or photochemically induced
changes that sometimes lead to bleaching of the dyes or pigments in the
mounted specimen.

I have not been in a situation where I needed to preserve a slide
past my own estimate of maximum longevity, so Damar has sufficed. I have
prepared three liters of Gum Damar since 1967, and the most recent is still
fairly full. Each preparation has taken at least a year to finish. I do
not expect to make another, and I suspect that you have just decided that
you will NOT begin such an ordeal.

So! If you are using Balsam, you will have to wait for 6mos before
you can stack the slides as the edge seal takes that long to set.

If you use any mountant with Xylene as the vehicle, you will have
the advantage of longevity, but you must use the mountant resin at close to
saturation, because that is the equilibrium 'sought' by the mixture and you
may have to wait longer for complete setting. Too much vehicle, and the
equilibrium volume will be smaller than desired and air may ultimately be
drawn into the space between slide and cover glass. The concentration of
resin is an important key to long-term success.

The other complication is the volume of the mountant used. This can
be solved by careful application of the mountant so that the minimum
required volume of saturated mountant is used. Following this, I have
always weighted the cover glasses with rectangular lead blocks ( with a
square cross section!) measuring 2-3mm less on a side than the cover glass
dimensions. Steel blocks cut to appropriate dimensions can be used with
fewer parenthetic comments and footnotes and subsequently cleaned when they
become contaminated with resin. These weights insure that a standard volume
of mountant seals each cover glass. NOTE A REAL DANGER POINT: When
training a technician who may perform the bulk of the work, it is important
to emphasize the archivists rationale for each step so that when the
mountant reservoir (normally in a convenient, but NOT tightly stoppered
container becomes too viscous, the proper, minimum, quantity of solvent is
added to recalibrate the viscosity. Alternatively, you can choose a better
reservoir than that which is commercially available or the dropper bottle
that is supplied with some mountants. Do not attempt to seal the open
reservoir with grease of any kind. I have often stored the reservoir in a
fume hood in a screw capped (cap is Teflon lined!) glass jar, large enough
to hold a small narrow-mouth container of vehicle-soaked cotton which I
maintain with Pasteur pipetted vehicle. This works nicely when the mountant
is not used up rapidly. In situations where a fume hood was NOT available,
I have NOT used the jar WITH the cotton-soaked vehicle - just the jar alone!
If I am to leave a container of mountant for any length of time, I record
the last level and date on a small label applied to the side so that when I
take it up again I can easily recalibrate the volume.

There may be some suggestions to paint the edges of the cover
glasses. This can, of course, retard the evaporation of the
solvent/vehicle, but, since most 'paints' are susceptible to the effects of
the organic solvents mentioned here, a retardation is all that can be
expected. AND, one must still wait until the edge seal is complete.

When properly prepared, and after edge sealing has completed, a
cover glass and slide will behave, in most situations, as a unit object.

For all that you are considering, I can recommend the following
book: Lillie, R.D.(1965), Histopathologic Technic and Practical
Histochemistry(3d Ed.), McGraw-Hill Book, Co., London [for you], pp91-106
[section on mounting media INCLUDING a brief paragraph on mounting diatoms
that is all about indices of refraction]. You will also find much if you
search among Gurr's books and papers. When searching the old literature,
pause when you find the term, cryptogamic.

My final recommendation is to check in town for a world-famous
institute of learning. I bet that if browsing the stacks is still
permitted, you can find something of real value in Lee, Vade Mecum(?); Gray,
Microtomist's Formulary and Guide; and, again, something by Gurr.

Good luck, archiving is special.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


} ----------
} From: Micha Bayer
} Sent: Monday, July 2, 2001 10:52 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: DPX versus Canada Balsam
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
}
} I am in the process of figuring out a way of permanently mounting
} desmids (unicellular green algae with a cellulose cell wall) and was
} wondering about the relative benefits of DPX and Canada balsam as
} mounting media.
}
} It appears that no-one in our field has done this kind of thing before
} (please tell me if I am wrong) and we have to guess our way through
} this.
}
} Is Canada balsam still used? Which mountant has the better long
} term and optical characteristics in your experience? Has anyone got
} DPX slides which are older than say 20-30 years, and are they still
} acceptable?
}
} many thanks
}
} Micha Bayer
}
} ______________________________
}
} Dr. Micha Bayer
} Royal Botanic Garden Edinburgh
} 20A Inverleith Row
} Edinburgh EH3 5LR
} Scotland, U.K.
} Tel. (+44) (0)131-248 2915 or 248 2965
} Fax (+44) (0)131-248 2901
} Project Homepage at http://www.rbge.org.uk/ADIAC/
} RBGE home page at http://www.rbge.org.uk
} ______________________________
}
}


From daemon Tue Jul 3 11:12:10 2001



From: Michael D. Standing :      Michael_Standing-at-byu.edu
Date: Tue, 03 Jul 2001 10:08:13 -0600
Subject: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sally,

The book HISTOLOGICAL TECHNIQUES FOR ELECTRON MICROSCOPY by Daniel C. Pease
(second edition) published in 1964 by Academic Press, gives a brief history
of the use of Toluidine Blue as a stain for survey sections on pages
259-260. It states, "In a symposium discussion of a paper by Mercer (1963),
Charles and Meek separately emphasized the usefulness of alkaline toluidine
blue. If a 1% solution dissolved in 1% borax is used at 85 degrees C,
staining occurs in Araldite in 1-2 minutes."

I hope this helps answer your question.


======================================
Michael D. Standing
BYU Microscopy Lab
401 WIDB
Brigham Young University
Provo, UT 84602

e-mail: Michael_Standing-at-byu.edu
phone: (801) 378-4011
fax: (801) 378-3937
======================================

-----Original Message-----
} From: Sally Stowe [mailto:STOWE-at-rsbs.anu.edu.au]
Sent: Tuesday, July 03, 2001 1:38 AM
To: Microscopy-at-sparc5.microscopy.com



Somebody just asked me the reference for toluidine blue/sodium borate (1%
each) which is our routine LM stain - I cant find an attribution in any of
about 10 books. Can anybody help? This person should not be forgotten!
One suggestion was Alsop?

thanks

Sally



Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU





From daemon Tue Jul 3 12:07:15 2001



From: Petersen, Maureen A. :      Mape-at-mail.ifas.ufl.edu
Date: Tue, 3 Jul 2001 13:00:51 -0400
Subject: TEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} -----Original Message-----
} From: Petersen, Maureen A.
} Sent: Tuesday, July 03, 2001 11:52 AM
} To: 'Microspy-at-MSA.Microscopy.Com'
} Subject: TEM available
}
} List members:
}
} The Department of Plant Pathology, University of Florida, has a Hitachi
} 600 TEM available.
}
} This microscope has not been on a service contract in recent months, and
} went down on me, so I can not say it is currently operating. I THINK I
} know where the problem lies, but it needs an engineer. Please contact me
} off list, and I will give you more specific information.
}
} University of Florida labs, then State of Florida labs, have first dibs.
} But although the Hitachi has been my companion for years, I recognize that
} it may not be a hot item, so no one should hesitate to inquire.
}
} The microscope is available for the cost of removing it. We have another
} scope coming in, so time is limited to arrange to take it. If nothing
} else, it could be a good parts scope.
}
} All interested parties, please contact me ASAP.
}
} Maureen Petersen
} University of Florida
} Department of Plant Pathology
} Gainesville, FL 32611
}
} phone (352) 392-0634


From daemon Tue Jul 3 12:07:17 2001



From: William McManus :      billemac-at-biology.usu.edu
Date: Tue, 3 Jul 2001 11:02:37 -0600
Subject: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sally:

Please tell me more about this stain. Why are you using it and what are
it's advantages?
Bill


William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Sally Stowe [mailto:STOWE-at-rsbs.anu.edu.au]
Sent: Tuesday, July 03, 2001 1:38 AM
To: Microscopy-at-sparc5.microscopy.com



Somebody just asked me the reference for toluidine blue/sodium borate
(1% each) which is our routine LM stain - I cant find an attribution in
any of about 10 books. Can anybody help? This person should not be
forgotten! One suggestion was Alsop?

thanks

Sally



Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU






From daemon Tue Jul 3 12:22:54 2001



From: marian miller :      millermn-at-email.uc.edu
Date: Tue, 03 Jul 2001 13:17:27 -0400
Subject: sperm electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


does someone have a good, and easy method for preserving mouse sperm for
transmission microscopy



From daemon Tue Jul 3 13:22:18 2001



From: David Burton :      dbuw-at-u.washington.edu
Date: Tue, 3 Jul 2001 13:16:35 -0500
Subject: Zeiss lamp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I have a Zeiss OPMI surgical microscope. I bought it used as-is.
} It came with a bulb that didn't last. The filament broke away
} from the support wires. A fine spiral of thinner wire wound around
} the support wires seemingly to help support the filament, and it
} had spiralled off the supports.
}
} Fortunately my junk pile revealed a replacement bulb. However,
} after the 'scope took a trip in the car to demo for some
} kindergarteners, its filament also broke and the thin wires
} spiralled away.
}
} $45 at Bulbdirect.com and I'm back to a working bulb and a spare.
} What should I be doing to protect myself against early lamp
} failure?

The design of this lamp with the long filament holders makes this lamp prone
to filament breakage from impact when the lamp is cold. If you have had
problems when transporting the microscope try removing the bulb and storing
it in its box. Are you using the 30 watt bulb? It was common for a 50 watt
bulb to be installed in this microscope in error.


Cool-down without movement for 20 minutes? NOPE.

Remove
} lamps before driving around with the 'scope? YEP.

Are short bursts
} of usage (5-10 minutes each) to blame? NOT AT ALL.


From daemon Tue Jul 3 13:25:31 2001



From: Battjes, Kevin :      battjes-at-impactanalytical.com
Date: Tue, 3 Jul 2001 08:32:28 -0400
Subject: SEM Service Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,
I agree with Richard, it's a parts and service know-how issue. We also have
an Amray (1820). Amray didn't discontinue our service, KLA-Tencor did and
unfortunately, that's the end of the line for a fine brand of SEM's.

We also have added EDS to our instrument recently and expect to get many
more years of service from it. We too, contracted with SEMtech Solutions
for service (ex Amray guys) and get service from the same engineer that we
had with Amray. A very comfortable situation for us.

If the instrument meets your needs and has parts and service talent
available, keep it running as long as it is economical and reliable.

(No vested interest in SEMtech, unless you consider I want them to stay in
business so they can fix my instrument!)


Kevin Battjes
Impact Analytical
Michigan Molecular Institute
1910 W. St Andrews Road
Midland MI 48640



-----Original Message-----
} From: Bertaska, Richard [mailto:BertaskaR-at-corning.com]
Sent: Tuesday, July 03, 2001 7:07 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


SEM service life is based on parts availability and support.

Many older SEMs are still supported because the failing parts/components are
still manufactured. Fortunately manufacturers used many readily available
parts. Also many institutions/businesses have purchased new equipment and
"retired the old". Fortunately there are some enterprising individuals who
recognized a niche and began collecting "abandoned" SEMs. These individuals
now provide a necessary parts/service to SEM industry.

As for support....if you can do much of your own you are smart. For those of
us who neither have the time or expertise we need to seek out the
experts/entrepreneurs.

I currently manage three SEMs. The oldest is AMRAY1600 purchased in 1984.
It is a workhorse for us. Despite minimal maintenance and no service
contracts over the past years it has performed well. Recently a number of
components began to fail. It was suggested that the system be retired and
replaced.

I found expertise in SEMTech Solutions Inc. During the past year we have
replaced several aging components, added digital imaging, and moved the SEM
to another building for access to multiple users. EDS upgrade is also being
considered. As long as parts are available I expect the system to perform
another 20 years!

SEMTech provided expertise and parts. I would highly recommend them for
AMRAY instrument service.

c/o Jim Peterson
PO Box 2155
Natick MA 01760
tel: (978) 663-9822


I have no vested interest in the company. I am simply a truly satisfied
customer with an excellent service provider.

Finding other experts for other SEM instrumentation may be difficult.
However, if the SEM community needs/supports it, I think there are
individuals prepared to provide it.

Perhaps the reason these smaller new EDS ventures have been able to
establish themselves is because the larger established businesses no longer
listened/responded to their customer needs. A similar circumstance could
evolve with SEM maintenance.


The question poses another thought: How could the SEM community "inventory"
all SEMs by brand, model, age, location, condition, and status. Thus all
SEMs being accounted for could effectively provide the potential inventory
parts list and availability.





From daemon Tue Jul 3 13:43:52 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 3 Jul 2001 14:30:50 -0400
Subject: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Original Message-----
} From: Jensen, Karen
Sent: Tuesday, July 03, 2001 2:30 PM
To: 'Sally Stowe'



Somebody just asked me the reference for toluidine blue/sodium borate (1%
each) which is our routine LM stain - I cant find an attribution in any of
about 10 books. Can anybody help? This person should not be forgotten!
One suggestion was Alsop?

thanks

Sally



Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU





From daemon Tue Jul 3 13:43:52 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Tue, 3 Jul 2001 14:24:06 -0400
Subject: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sally:

I think the reference is:

Richardson, K.C., Jarret, L., Finke, E.H.: Embedding in epoxy resins for
ultrathin sectioning in electron microscopy. Stain Techn. 35:313, 1960


Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642

-----Original Message-----
} From: Sally Stowe [mailto:STOWE-at-rsbs.anu.edu.au]
Sent: Tuesday, July 03, 2001 3:38 AM
To: Microscopy-at-sparc5.microscopy.com



Somebody just asked me the reference for toluidine blue/sodium borate (1%
each) which is our routine LM stain - I cant find an attribution in any of
about 10 books. Can anybody help? This person should not be forgotten!
One suggestion was Alsop?

thanks

Sally



Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU





From daemon Tue Jul 3 16:24:56 2001



From: Zhaojie Zhang :      Zhaojie-Zhang-at-mail.omrf.ouhsc.edu
Date: Tue, 3 Jul 2001 16:15:17 -0500
Subject: IEM for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike:

It might be difficult to see the 5 nm gold with SEM (at least in my
experience) -- try to put a drop of 1:10 diluted 5 nm gold on a sample
holder/stub, air dry and to see if you can identify the gold particles with
SEM, if yes, great! if not, try 10 nm or 15 nm. I am sure 15 nm will do it.

Check out the paper:"Localization of myosin on sperm-cell-associated
membrances of tobacco" by Zhang Z, HQ Tian and SD Russell on Protoplasma
(1999) 208:123-128, in which Immunogold SEM with 15 nm gold is used to
localize myosin on cell surface.
http://www.springer.at/springer.py?Page=47&Key=87&cat=9&id_abstract=5759&id_
volume=641&id_journal=4

Let me know if you could not find the paper. I will be happy to send you a
copy.

Zhaojie Zhang
Oklahoma Medical Research Foundation
Oklahoma City, OK 73104

-----Original Message-----
} From: Mike Delannoy
To: microscopy-at-sparc5.microscopy.com
Sent: 7/3/01 9:39 AM


Hello Microscopists,

We are doing an IEM for SEM with bacteria and would appreciate any
comments. We are looking for surface label 5nm gold with a FIESEM. Our
protocol is:

3% PF 0.05% GA fix PO4 buffer

rinse

Bock in 1%NGS

rinse

Primary ABY incubation O/N cold

rinse

Secondary gold conjucated ABY 1hr

rinse

Hard fix 2% GA same buffer

rinse

2% UA filtered DH2O

Dehydration

Critical Point Drying

Our questions are is the UA enough to stablize the lipds throughout the
dehydration and cpd? We believe osmium would strip off the gold and
tannic acid may make small precipitates similar in size to the gold.
We will use in-lens and backscatter to view the gold. Any other
suggesstions would be appreciated.

Thank you

Mike D



From daemon Tue Jul 3 17:17:25 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 03 Jul 2001 18:17:56 -0400
Subject: Re: Bulb shock?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
My old Zeiss dissecting scope (focal distance about 10" - I love it) has
a voltage controller for the lamp. There is a detent in the middle of
the range which gives the rated voltage (in this case 6V). It can be
considerably over-driven for unusual circumstances, but if you leave it
there, very short bulb-life is the result. Check your power supply output.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

John Foust wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I have a Zeiss OPMI surgical microscope. I bought it used as-is.
} It came with a bulb that didn't last. The filament broke away
} from the support wires. A fine spiral of thinner wire wound around
} the support wires seemingly to help support the filament, and it
} had spiralled off the supports.
}
} Fortunately my junk pile revealed a replacement bulb. However,
} after the 'scope took a trip in the car to demo for some
} kindergarteners, its filament also broke and the thin wires
} spiralled away.
}
} $45 at Bulbdirect.com and I'm back to a working bulb and a spare.
} What should I be doing to protect myself against early lamp
} failure? Cool-down without movement for 20 minutes? Remove
} lamps before driving around with the 'scope? Are short bursts
} of usage (5-10 minutes each) to blame?
}
} - John
}
} P.S. Dr. Monson: Love the stories. Keep in mind that all
} seemingly newbies on the 'net might be learning very slowly
} over time, just for fun, and without benefit of a degree program.
} The Web is our Oracle, but I'll try to pay attention during class.
} Please do not hit me with eraser, chalk or ruler.
}
}
}
}



From daemon Tue Jul 3 18:15:51 2001



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Wed, 4 Jul 2001 14:52:47 +0930 (CST)
Subject: any plant anatomists?

Contents Retrieved from Microscopy Listserver Archives
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-------- Original Message --------




-------- Original Message --------


Ken,

Yeah, I know & it looks it.

Earl

----- Original Message -----
} From: "Ken Converse" {qualityimages-at-netrax.net}
To: "MSA, listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, July 03, 2001 4:15 PM


Hello,

I have a query relating to a project, which is examining the effects of
citrus rind oils (mainly terpenoid in composition) on rind tissue. Rind
cells appear to 'plasmolyse', membranes degenerate, cell walls collapse and
swell. Oil appears to move apo- and symplastically.

My question is: Has anyone examined the effects of similar oils on plant
tissues before? Were observations similar to those described above?
What is the detailed ultrastructural process of plasmolysis?

Please email me directly.

thankyou, Toby Knight.


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
Adelaide University
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064
AUSTRALIA

Tel: +61 8 8303 6668
: +61 8 8303 7242 (HVO)
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------




From daemon Wed Jul 4 10:04:23 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 4 Jul 2001 08:41:45 -0600
Subject: Re: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Let me add one possible source to the "saw-tooth edge" problem:

60 Hz noise from the power supply (50Hz in other parts of the world).

You can check that by looking up resolution and frame rate and measuring the
"wavelength" of the oscillations. Example:

You are looking at 500 lines and 10 frames per second. This gives you around
500 lines per second. A 60 Hz signal should have a "wavelength" of around 8
lines, a 50 Hz signal of around 10 lines ( # of lines x frame rate /
frequency). You can also take the measured wavelength and caculate the
frequency of the vibration. This can give you some insight into the source
of the vibration or interference.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Steve Chapman [mailto:PROTRAIN-at-compuserve.com]
Sent: Tuesday, July 03, 2001 7:06 AM
To: American Soc


Hi

As the image is in a SEM is created by lines of information, many problems
exhibit themselves by giving saw tooth edges to the lines. The slower you
scan the better the instrument is at synchronizing out the interference.

The most common reasons for the above are:-

1. Vibration
2. External magnetic fields

If we consider operating parameters we may be able to track this down.
Magnetic fields will have a greater affect at lower accelerating voltages
and at longer working distances, in both cases the final lens strength is
reduced allowing the external field to overcome what protection the lens
field provides. Try working at the shortest possible working distance and
at the highest kV, probably about 5mm at 30kV. If this reduces or removes
the problem it would be an external field that is the culprit. Another
indication of an external field problem is the TV rate scan will have a sub
ripple that will work its way down the screen.

We need to know if you have been able to take good photographs above 5,000X
in the past. If so we then can assume the problem is a result of a change
in the laboratory; a new piece of equipment is giving off the field. This
may be many feet away, the field effect is according to the inverse square
law. Try going round switching off new equipment and see if that helps. I
have seen problems due to incorrect wiring of new equipment so if you find
a field source have a technician check the wiring before going any further.

Please come back if you need more help.

Steve Chapman
Senior Consultant, Protrain
For professional training in SEM, TEM and EDX world wide
www.emcourses.com


From daemon Wed Jul 4 10:06:07 2001



From: Simon Dumbill :      simon.dumbill-at-aeat.co.uk
Date: Wed, 4 Jul 2001 10:02:59 -0500
Subject: Philips EM300/301 sample holders

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

If there are any Philips EM300 series TEM users out there who are in
need of sample holders, please contact me. I have four double-tilt
holders and a double-tilt LN2 stage that need new homes.

Simon

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}

Dr Simon Dumbill
Team Leader, Microstructural Characterisation
AEA Technology Nuclear Science
B7, Windscale
Seascale
Cumbria CA20 1PF

Tel: +44 (0)19467 72235
Fax: +44 (0)19467 72606

Email: Simon.Dumbill-at-aeat.co.uk

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}




***********************************************************************
This transmission contains information which may be confidential and
which may also be privileged. It is intended for the named addressee
only. Unless you are the named addressee, or authorised to receive it
on behalf of the addressee you may not copy or use it, or disclose it
to anyone else. If you have received this transmission in error please
contact the sender. Thank you for your cooperation.
***********************************************************************

For more information about AEA Technology please visit our website at
http://www.aeat.co.uk

AEA Technology plc registered office 329 Harwell, Didcot, Oxfordshire OX11 0QJ.
Registered in England and Wales, number 3095862.




From daemon Wed Jul 4 15:16:48 2001



From: Robert Wieland :      wieland-at-me.udel.edu
Date: Wed, 4 Jul 2001 16:08:41 -0400 (EDT)
Subject: RE: SEM Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another possible source of this is any "spare" oscillators. If The TV
scan rate is not generated by the SEM's "main" scan oscillators, those
oscillators can sometimes be left running when working in TV frame rate.
These oscillators then put noise of their frequency on the power busses,
from which it leaks into the scan drive circuits, contaminating the scan.
With your scan coils doing "contaminated" scanning, and your monitor or
video capture board doing "pure" scanning, you get sawtooth edges.
Read the manual very carefully to see if there isn't some way to stop
the "main" scan oscillators. Often this info is in the "testing &
calibration" section, as you typically need to stop these oscillators to
get an "uncontaminated" true reading of the power supply ripple, to verify
the supply is OK.


Robert Wieland wieland-at-me.udel.edu
No one of us is as smart as all of us. Japanese saying



From daemon Wed Jul 4 15:39:43 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 05 Jul 2001 08:38:48 -0500
Subject: Re: CONFIDENCIAL BUSSINES PROPOSAL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


.. and knowing Murphy, it's probably a combination of more than one effect.

You should be able to test the suggestion below by watching the ripple and
change the scan settings on the "spare" oscillator. If the ripple changes,
you found the problem.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

-----Original Message-----
} From: Robert Wieland [mailto:wieland-at-ME.UDel.Edu]
Sent: Wednesday, July 04, 2001 2:09 PM
To: Mike Bode
Cc: 'Microscopy-at-MSA.Microscopy.Com'










At 07:15 PM 7/4/01 -0700, sammy eze wrote:
} ATTN: MANAGING DIRECTOR/CEO.
} REQUEST FOR AN URGENT CONFIDENTIAL BUSINESS RELATIONSHIP

Here's the Nigerian Embassy's page on this years-old scam:

http://www.nigerianembassy.nl/letters.htm

This scam accounts for a significant percentage of their GNP.

- John



From daemon Thu Jul 5 08:49:49 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 5 Jul 2001 06:46:50 -0700 (PDT)
Subject: Re: sperm electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Members:
Just in case it is not obvious to all, the posting below is a scam. It
recently appeared on another list I subscribe to, also.

Maureen Petersen
University of Florida

-----Original Message-----
} From: sammy eze [mailto:kenno-at-37.com]
Sent: Wednesday, July 04, 2001 10:13 PM
To: kenayaya-at-amebo.com




Marian:
I don't have the references directly available, but the papers of A. Kent
Christensen are a very good place to start. I did, however, come across an
unattributed method in Hayat's book "Fixation for Electron Microscopy"
(badly damaged in a lab flood, but still legible for the most part).
Glut (8%) 25ml
Paraform (105) 25ml
Picric Acid 0.02g
0.1M phosphate buffer to make 100ml (total fixative volume)

Collect first two drops directly into 20ml sol A (above) at RT. Discard
proteinaceous seminal plasma which are formed rapidly due to coagulation.
Centrifuge the remaining nearly pure cellular suspension at 1000rpm for
10min (total duration of fixation is ~25m). Discard the supernatant and
wash the pellet in phosphate buffer for 15m. Postfix the pellet in 1% Os)4
in 0.1M phosphate buffer(pH 7.2) for 15 min at RT.

I have _no_ idea if this is an optimal method, but it's all I can find
quickly. Again, I would refer you to Dr. Christensen's work.

Roger
On Tue, 03 Jul 2001 13:17:27 -0400, marian miller wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| does someone have a good, and easy method for preserving mouse sperm for
| transmission microscopy
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Thu Jul 5 09:06:21 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 5 Jul 2001 10:01:43 -0400
Subject: RE: DPX versus Canada Balsam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chuck,

Very simple answer, but I, as usual, can't explain it briefly.

We all get married to what we learn originally, because what we
learn is the foundation of our individualized empiric relationship with
science. Changes have to be couched in terms that are similar to those used
to explain our empiric base, OR, we must be forced!

"We use Canada Balsam, because...." Or, as in my case, "I
have found Gum Damar to be the best!"

AND, I was just wondering about plastic mountants a minute
ago in response to a message from John Bozzola, and was trying, again, to
find the specific components of "EPON" and its index of refraction. No
success.

Am I familiar with thermoplastics? Yes, from many years
ago. The Cargille thermoplastics of today? Not specifically. Reason?
Lack of incentive. They were much more expensive, and I was already
'married'. I had 15 year-old preparations with no observable defects, and I
was not forced to investigate that alternative for safety reasons either by
internal safety flags or external. I had been 'sniffing' for 20 years - one
thing and another, and I got 'smarter' every day. ?????

If I had an archival project, such as the one that initiated
this thread, I would surely investigate the thermoplastic mountants, in
spite of the fact that the cost is MUCH higher/slide. The advantages are
obvious.

My reason would be simple, and in concert with your
consternation. I would want the insurance of vehicle absence to assure
longevity without concern for evaporation and volume shrinkage. I would
still minimize usage, but application volumes presumably could be rigidly
controlled by applying to each coverglass a pre-determined volume that would
form the correct seal, without the otherwise obligatory weight and trimming
of excess, and then 'picking' up the cover glass with the inverted slide.

Pathologists would NOT change unless forced to contend with
a vehicle/solvent safety issue. Real long-term archiving apparently is NOT
the greatest issue with them, and pathology is a REAL customer.

Sometimes, but not often, I am shocked by a loss of memory.
In this case, I would certainly abbreviate my previous to Micha even more
and suggest that he look carefully at the thermoplastic mountants. Further,
now that a safety issue has been raised, I can't think why I would not
consider/try them for my routine work.

Thanks for the reminder and the gentle wake-up,

Regards,

Fred Monson

} ----------
} From: Garber, Charles A.
} Sent: Tuesday, July 3, 2001 6:22 PM
} To: Monson, Fred C.
} Subject: RE: DPX versus Canada Balsam
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Thanks for the great lesson, you must be a fantastic teacher in front of a
} class. I have a question: Are you familiar with the Cargille Optical
} Quality Thermoplastics, as shown on URL
} http://www.2spi.com/catalog/ltmic/opt-thermo.html
}
} Now these are all thermoplastic materials, there is no solvent that can
} evaporate. I know that people use these materials for doing some of the
} same kinds of mounting you described in your posting. I am trying to
} understand why, if the solvent can be the potential cause for so many
} problems, why people don't use these optical quality thermoplastics?
}
} Presumably there is some simple answer, and then I would understand......
}
} Chuck
} -------- REPLY, Original message follows --------
}
} } Date: Tuesday, 03-Jul-01 12:02 PM
} }
} } From: Monson, Fred C. \ Internet: (fmonson-at-wcupa.edu)
} } To: MICROSCOPY BB \ Internet:
} } (microscopy-at-sparc5.microscopy.com)
} } cc: Micha Bayer \ Internet: (m.bayer-at-rbge.org.uk)
} }
} } Subject: RE: DPX versus Canada Balsam
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To
} } Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-
} Line
} } Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Good Morning Micha,
} } First, I can only provide my experience on this matter.
} } Second, I will summarize what I have trundled on about below for
} the
} } purpose of those who are interested in the destination, not the journey.
} }
} } 0. Yes, I have seen "Permount" preps of this age,
} so
} I'm
} } certain that mountant, properly applied, will work. The slides I made
} as
} a
} } student in 1964 with many different dyes and stains - mountant Gum Damar
} -
} are
} } still as 'perfect' as my "B" illustrated.
} } 1. With respect to vapor pressure:
} Xylene {Toluene {Benzene
} } 2. Three problems with mountants:
} } a. Resin concentration must be close to
} saturation
} } to minimize evaporative changes in volume after application.
} } b. Mountant volume under cover glass must
} be
} close
} } to minimum (I standardize this with weights after application!)
} } c. Resin composition must NOT cause
} 'unacceptable'
} } changes in specimen (e.g., acidity?).
} }
} } For years, I have used a natural resin called Gum Damar (or
} Dammar
} !)
} } (apparently 'mined' from the same quarter of the planet as hematoxylin),
} } solubilized in Xylene (Xylene {Toluene {Benzene(vapor pressure). This
} } preparation has failed for only one reason that I can discern - too much
} } solvent in the drop(s) used to seal the cover glass. The major problem
} is
} loss
} } of solvent via evaporation.
} }
} } Most of the mountants that are commercially available are
} solubilized
} } in Toluene, thus, they tend to 'dry' somewhat faster than the prep I
} have
} used.
} } While the commercial preparations tend to dry faster, they do not
} appear
} to
} } suffer from the other problem apparent in natural preps like Damar that
} is
} } acidification and/or photochemically induced changes that sometimes lead
} to
} } bleaching of the dyes or pigments in the mounted specimen.
} }
} } I have not been in a situation where I needed to preserve a
} slide
} past
} } my own estimate of maximum longevity, so Damar has sufficed. I have
} prepared
} } three liters of Gum Damar since 1967, and the most recent is still
} fairly
} full.
} } Each preparation has taken at least a year to finish. I do not expect
} to
} make
} } another, and I suspect that you have just decided that you will NOT
} begin
} such
} } an ordeal.
} }
} } So! If you are using Balsam, you will have to wait for 6mos
} before you
} } can stack the slides as the edge seal takes that long to set.
} }
} } If you use any mountant with Xylene as the vehicle, you will
} have
} the
} } advantage of longevity, but you must use the mountant resin at close to
} } saturation, because that is the equilibrium 'sought' by the mixture and
} you may
} } have to wait longer for complete setting. Too much vehicle, and the
} } equilibrium volume will be smaller than desired and air may ultimately
} be
} drawn
} } into the space between slide and cover glass. The concentration of
} resin
} is an
} } important key to long-term success.
} }
} } The other complication is the volume of the mountant used. This
} can be
} } solved by careful application of the mountant so that the minimum
} required
} } volume of saturated mountant is used. Following this, I have always
} weighted
} } the cover glasses with rectangular lead blocks ( with a square cross
} section!)
} } measuring 2-3mm less on a side than the cover glass dimensions. Steel
} blocks
} } cut to appropriate dimensions can be used with fewer parenthetic
} comments
} and
} } footnotes and subsequently cleaned when they become contaminated with
} resin.
} } These weights insure that a standard volume of mountant seals each cover
} glass.
} } NOTE A REAL DANGER POINT: When training a technician who may perform
} the
} bulk
} } of the work, it is important to emphasize the archivists rationale for
} each
} } step so that when the mountant reservoir (normally in a convenient, but
} NOT
} } tightly stoppered container becomes too viscous, the proper, minimum,
} quantity
} } of solvent is added to recalibrate the viscosity. Alternatively, you
} can
} } choose a better reservoir than that which is commercially available or
} the
} } dropper bottle that is supplied with some mountants. Do not attempt to
} seal
} } the open reservoir with grease of any kind. I have often stored the
} reservoir
} } in a fume hood in a screw capped (cap is Teflon lined!) glass jar,
} large
} } enough to hold a small narrow-mouth container of vehicle-soaked cotton
} which I
} } maintain with Pasteur pipetted vehicle. This works nicely when the
} mountant is
} } not used up rapidly. In situations where a fume hood was NOT available,
} I
} have
} } NOT used the jar WITH the cotton-soaked vehicle - just the jar alone! If
} I
} am
} } to leave a container of mountant for any length of time, I record the
} last
} } level and date on a small label applied to the side so that when I take
} it
} up
} } again I can easily recalibrate the volume.
} }
} } There may be some suggestions to paint the edges of the cover
} glasses.
} } This can, of course, retard the evaporation of the solvent/vehicle,
} but,
} since
} } most 'paints' are susceptible to the effects of the organic solvents
} mentioned
} } here, a retardation is all that can be expected. AND, one must still
} wait
} } until the edge seal is complete.
} }
} } When properly prepared, and after edge sealing has completed, a
} cover
} } glass and slide will behave, in most situations, as a unit object.
} }
} } For all that you are considering, I can recommend the following
} book:
} } Lillie, R.D.(1965), Histopathologic Technic and Practical Histochemistry
} (3d
} } Ed.), McGraw-Hill Book, Co., London [for you], pp91-106 [section on
} mounting
} } media INCLUDING a brief paragraph on mounting diatoms that is all about
} indices
} } of refraction]. You will also find much if you search among Gurr's
} books
} and
} } papers. When searching the old literature, pause when you find the
} term,
} } cryptogamic.
} }
} } My final recommendation is to check in town for a world-famous
} } institute of learning. I bet that if browsing the stacks is still
} permitted,
} } you can find something of real value in Lee, Vade Mecum(?); Gray,
} Microtomist's
} } Formulary and Guide; and, again, something by Gurr.
} }
} } Good luck, archiving is special.
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } West Chester University of Pennsylvania
} } Center for Advanced Scientific Imaging (CASI)
} } Schmucker II Science Center (Room: SS024(Basement))
} } South Church Street
} } West Chester, PA, 19383
} } MailDrop: Department of Geology/Astronomy
} } Phone: 610-738-0437
} } Fax: 610-436-3036
} } email: fmonson-at-wcupa.edu
} } Please call before visiting.
} }
} }
} } } ----------
} } } From: Micha Bayer
} } } Sent: Monday, July 2, 2001 10:52 AM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: DPX versus Canada Balsam
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Hi all,
} } }
} } } I am in the process of figuring out a way of permanently mounting
} } } desmids (unicellular green algae with a cellulose cell wall) and was
} } } wondering about the relative benefits of DPX and Canada balsam as
} } } mounting media.
} } }
} } } It appears that no-one in our field has done this kind of thing before
}
} } } (please tell me if I am wrong) and we have to guess our way through
} } } this.
} } }
} } } Is Canada balsam still used? Which mountant has the better long
} } } term and optical characteristics in your experience? Has anyone got
} } } DPX slides which are older than say 20-30 years, and are they still
} } } acceptable?
} } }
} } } many thanks
} } }
} } } Micha Bayer
} } }
} } } ______________________________
} } }
} } } Dr. Micha Bayer
} } } Royal Botanic Garden Edinburgh
} } } 20A Inverleith Row
} } } Edinburgh EH3 5LR
} } } Scotland, U.K.
} } } Tel. (+44) (0)131-248 2915 or 248 2965
} } } Fax (+44) (0)131-248 2901
} } } Project Homepage at http://www.rbge.org.uk/ADIAC/
} } } RBGE home page at http://www.rbge.org.uk
} } } ______________________________
} } }
} } }
} }
}
} -------- REPLY, End of original message --------
}
}
}


From daemon Thu Jul 5 09:38:34 2001



From: JoAn Hudson :      hjoan-at-CLEMSON.EDU
Date: Thu, 05 Jul 2001 10:28:41 -0400
Subject: Welcome contributions for journal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MSA Members!

The MSA/Microsanalysis journal is seeking someone to become part of News
and Commentary by writing the "Net Notes" section for the journal. If you
are interested in being a part please send reply to JoAn Hudson at
hjoan-at-clemson.edu

Thank you.


From daemon Thu Jul 5 10:04:57 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Thu, 05 Jul 2001 10:01:42 -0500
Subject: Fwd: CONFIDENCIAL BUSSINES PROPOSAL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to those who pointed out that this is a scam! The two misspellings
in the subject heading gave them away (along with pretty much the whole
message contained within the email). Maybe they should not have sent it to
a list composed of people whose main talent is likely to be well-honed
powers of observation and visual data analysis.
Kristen

} } From popserve Wed Jul 4 21:23:06 2001
} Date: Wed, 4 Jul 2001 19:03:50 -0700
} X-Mailer: MIME-tools 4.104 (Entity 4.116)
} X-Originating-Ip: [63.103.140.102]
} From: "sammy eze" {kenno-at-37.com}
} To: kenayaya-at-amebo.com
} Subject: CONFIDENCIAL BUSSINES PROPOSAL
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Thu Jul 5 10:04:57 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Thu, 05 Jul 2001 09:57:30 -0500
Subject: Re: sperm electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This topic was discussed a few months ago with regards to a question about
fixing and embedding human sperm. Here's the result of that, along with the
name and information of the generous soul who shared.
Kristen

Our lab routinely embeds rabbit and horse spermatozoa. You really
will need to centrifuge the ejaculate into a reasonably dense pellet.
I wouldn't worry about excess "junk".
We fix the ejaculate in 4% glutaraldehyde, 5% sucrose in 1M
cacodylate for 24hr. Wash with buffer.
Osmicate in 1% OsO4 for 1.5hr. Wash in buffer, centrifuge then
embed in 2% low melting point agarose(Sigma A5030), 2% sucrose in
buffer. This does very well in 1cc syringes that have been cut off at
both ends and the plunger cut even with the bottom so that you can
fill them to about 0.5cc and they will fit in a centrifuge tube. You
can warm and mix the sperm and agarose in the syringe, keep warm and
centrifuge, then push out a perfect pellet. We then use a standard
dehydration and epon protocol for TEM.
Sorry I don't have published reference for this. I have it as a
hand-me down protocol.
Jennifer
}
}
====================================
Jennifer Palmer
ARBL
Colorado State Univ
Ft. Collins, CO 80523-1683 , USA
voice:(970)491-1770
fax:(970)491-3557
jpalmer-at-cvmbs.colostate.edu
====================================


Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Thu Jul 5 10:12:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 5 Jul 2001 11:06:51 -0400
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Rosemary, I have been looking for that reference for years. I
lost it a long while ago, and I think that it IS the ONE!

By the way, I have always thought that 1865 was old! What happened?
Also, don't be surprised to discover in that article that the 1% tetraborate
buffer wasn't even new then.

For those who want more information, here's a little. [Choice: you
can go on with me or divert to a very instructive site:
http://members.pgonline.com/~bryand/dyes/class/dyeclass.htm]

There is also a good discussion with many references, though not
Rosemary's, in (another OLD book?): Lewis, P.R. and Knight, D.P.(1977),
Staining Methods for Sectioned Material, North-Holland Pub. Co., NY,NY,
ISBN: 0-7204-0606-4. Part of Series by Audrey M. Glauert, "Practical
Methods in Electron Microscopy", same publisher, series ISBN:
0-7204-4250-8.

Toluidine blue O is an thiazine dye that is related to methylene
blue(http://members.pgonline.com/~bryand/dyes/azures.htm). Many of these
dyes have long been known as "metachromatic" dyes, because they have the
property/characteristic ability to provide very nice multi-colored
(polychromatic) specimens. The mechanism is NOT perfectly understood, but
it is said to rest on the capacity of these dyes to form dye-dye aggregates
(pH sensitive because they are charge oriented and the dyes themselves are
usually amphoteric) which form generally on more acid and less acid or more
basic substances. Everything can be altered by regulating pH. For example,
I have used plain 1% aqueous thionin to stain DNA a 'crystal' azure and RNA
a muddy purple. I made slides in 1964 that are the same today as when I
produced them.

Azure B was once famous for it's use for this purpose. I didn't have any of
that, so I used the Organic chemistry that everyone else hated. I think,
from my brief, and cursory, comparative studies, that the toluidine blue
O/borax mixture 'cooks' much better than most others I tested.
Look carefully at the diversity of hue and color in the toluidine
blue O (used alone with borate) stained thin sections, and you will see why
it was (is with me and others(?)) so popular. I thought everyone knew about
it and just chose not to use it, though I have just discovered that Hayat
apparently doesn't mention it in his most recent general book on EM, nor in
others of his that I have on hand. Don't know why.

Hope this add help.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Rosemary White
} Sent: Tuesday, July 3, 2001 4:53 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Tol blue/borax
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Sally,
}
} In an old (1976) book on botanical microtechnique, there is a reference
} for
} 1% tol blue in 1% borax - Trump BF, Smuckler EA, Benditt EP (1961) A
} method
} for staining epoxy sections for light microscopy. J Ultrastr Res
} 5:343-348.
}
}
} There's also Mallory's borax methylene blue, maybe tol blue was a
} substitute for the methylene blue?
}
} cheers,
} Rosemary
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} phone 61-2-6246 5475 or 61-0402 835 973
} fax 61-2-6246 5000
} email r.white-at-pi.csiro.au
}
}
}
}


From daemon Thu Jul 5 11:27:11 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 5 Jul 2001 12:20:25 -0400
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sally,
To address the REAL attribution issue, one of us is going to have to
go to a REALLY good library that has these OLD journals back to the late
'50's. We may have to divide the labor, but the question is sufficiently
interesting and meritorious that we ALL should get it right, when asked.
Someone was responsible for the use of borax (Sodium tetraborate) with
Toluidine blue O (1% in 1%). Here are all the citations that Pease provides
and his [attributions]:

1. Trump, Schmuckler and Benditt (1961), J. Ultrastruc Res., 5:
343 [ 0.1% Toluidine blue O in 2.5% carbonate at pH 11 (confirmed in Lewis
and Knight below)]
2. Charles and Meek , G.R. separately in a discussion of paper
by Mercer(1963), J Microscp Sci., 81:179. [mentions 1% Toluidine blue O in
1% borax used at 85 degrees C]
3. Pease, D.C.(1964), Histological Techniques for Electron
Microscopy (2d), Academic Press, NY, NY, pp 256-262. [mentions on p. 260 in
2st paragraph the exact method that I use for staining as 'his' method -
"...flood the slide, cook until [edge] of drop [froths/]steams [or
dries]....". [This is a long discussion to which Pease devotes an entire
section, but one can just FEEL his impatience with the unimportance to HIM
of this issue. The book, after all, is NOT about light microscopy. The
semi-thin section is for orientation only, and hardly deserves any more
notice - especially if the light microscopists cannot SEE what is to be seen
in the best material ever handed them.]
4. In Kay, D.H.(ed)(1965), Techniques for electron
Microscopy(2d), F.A. Davis, Philadelphia, ISBN: [NOT given - is this book
pre-historic?!] The following is cited as THE reference for using Toluidine
blue O: Benscome, Stone, Latta, and Madden (1959), J. biophys. biochem.
cytol, 5: 508.

I have just looked in Geoffrey Meek's book (1970), Practical
Electron Microscopy for Biologists, Wiley_Interscience, NY, NY, p. 456-457.
ISBN: 0-471-59030-4. 1% in 1% with no citation, though in this book he has
purposely NOT used citations at all.

I have also consulted the Sorvall manual on "Thin Sectioning"(3d
Ed)(1973) as well as the SerVall manual "Thin Sectioning and Associated
Technics for Electron Microscopy"(1959) which apparently was distributed
with the MT-1. In the latter, only staining of Methacrylate sections was
given and no mention of 1% *bor* of any kind. In the former, 1% borate at
pH 4.5 is mentioned, but that is not relevant to this discussion.

I would bet that you might have some of these references. If you do
not, let me know, and I will dig them up, in old Philadelphia, in Ben
Franklin's old stacks (at the U. of P(ennsylvania) as we call it). I will
do it anyway, because I want to know, but I will do it quicker if you tell
me I'm keeping you waiting.

Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Sally Stowe
} Sent: Tuesday, July 3, 2001 3:37 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Tol blue/borax
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Somebody just asked me the reference for toluidine blue/sodium borate (1%
} each) which is our routine LM stain - I cant find an attribution in any of
} about 10 books. Can anybody help? This person should not be forgotten!
} One suggestion was Alsop?
}
} thanks
}
} Sally
}
}
}
} Dr Sally Stowe
} Facility Coordinator,
} ANU Electron Microscopy Unit
} Research School of Biological Sciences
} Australian National University
} Canberra ACT0200
} AUSTRALIA
} stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
} ph 02 6125 2743
} http://www.anu.edu.au/EMU
}
}
}
}
}


From daemon Thu Jul 5 12:21:50 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Thu, 05 Jul 2001 13:12:47 -0400
Subject: Dose resoponse curves ref

Contents Retrieved from Microscopy Listserver Archives
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Question
Has anyone seen references or know about research in which dose
responses of binding between fluorescently-labeled conjugates and cells
have been quantitatively analyzed using light microscopy with a camera
detection system and computer software.
Thanks in advance
Mike D



From daemon Thu Jul 5 12:56:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 5 Jul 2001 13:50:16 -0400
Subject: RE: IEM for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might want to consider enhancing the gold with silver, a la, Hayat,
M.A.(1995), Immunogold-Silver Staining, etc., CRC Press, Boca Raton, FL,
ISBN: 0-8493-2449-1. LC #: QR187.I482H39. I haven't done that, however.

Comforted that you are going to use BSD, I have used it for Ag impregnated
intercellular spaces, but the Ag concentrations were quite high. Can you do
EDS mapping as well?

Other source: http://www.ebsciences.com/papers/immusem.htm

Hermann R, Schwarz H, Müller M (1991). High precision immuno-scanning
electron microscopy using Fab fragments coupled to ultra-small colloidal
gold. J. Struct. Biol., 107(1): 38 - 47.
Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Mike Delannoy
} Sent: Tuesday, July 3, 2001 10:39 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: IEM for SEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Microscopists,
}
} We are doing an IEM for SEM with bacteria and would appreciate any
} comments. We are looking for surface label 5nm gold with a FIESEM. Our
} protocol is:
}
} 3% PF 0.05% GA fix PO4 buffer
}
} rinse
}
} Bock in 1%NGS
}
} rinse
}
} Primary ABY incubation O/N cold
}
} rinse
}
} Secondary gold conjucated ABY 1hr
}
} rinse
}
} Hard fix 2% GA same buffer
}
} rinse
}
} 2% UA filtered DH2O
}
} Dehydration
}
} Critical Point Drying
}
} Our questions are is the UA enough to stablize the lipds throughout the
} dehydration and cpd? We believe osmium would strip off the gold and
} tannic acid may make small precipitates similar in size to the gold.
} We will use in-lens and backscatter to view the gold. Any other
} suggesstions would be appreciated.
}
} Thank you
}
} Mike D
}
}
}


From daemon Thu Jul 5 15:17:52 2001



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Thu, 5 Jul 2001 15:10:31 -0500
Subject: bcip/NBT substrate and resin

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have experience with embedding, in resin, tissue fixed in 4%PF
that was labelled with an alkaline phospatase secondary ab reacted with the
substrate BCIF/NBT. The concern is the fading/dissolving/diffusion of the
substrate during processing.

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Thu Jul 5 16:27:37 2001



From: Damian :      neuberger1234-at-home.com
Date: Thu, 05 Jul 2001 16:24:20 -0500
Subject: Modifying a Prism

Contents Retrieved from Microscopy Listserver Archives
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I wrote about modifying a Nikon Diaphot 80/20 prism and received some good
suggestions (the 100/0) prism is no longer available from Nikon). When I
took the cover off the side of the microscope and removed the prism, I
discovered that one solution might be simply covering the binocular output
surface of the 80/20 prism. Would there be a problem with this
approach? I realize that I would only have 80% going to the specimen but
that may be tolerable. What would be the best way to block the laser beam
from exiting from the 20% prism surface? Black tape? Black paint? Black
metal ? Silver tape covered with black tape?

Thanks for the help

Damian



From daemon Thu Jul 5 17:46:49 2001



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 5 Jul 2001 15:39:06 -0700
Subject: Open Position

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The following position is open at Honeywell Engines and Systems in Phoenix,
AZ.

Microscopy Engineer/Technician

Honeywell Engines and Systems Materials Laboratory is looking for an
experienced engineer/technician knowledgeable in the operation of an
electron microprobe (EPMA) and scanning electron microscope (SEM) to support
production and development of aerospace materials. In depth knowledge of and
micro-x ray analysis methods using EDS and WDS is required.

Contacts:

Juan Trillo
602-231-2321
juan.trillo-at-honeywell.com

or

Dawn Hoffman
602-231-4510
dawn.hoffman-at-honeywell.com



From daemon Thu Jul 5 19:17:15 2001



From: Richard Gardiner :      rbgardiner-at-home.com
Date: Thu, 05 Jul 2001 20:12:31 -0400
Subject: Re: bcip/NBT stubstrate and resin

Contents Retrieved from Microscopy Listserver Archives
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Hank:

Your question is a good one and I also would be interested to know
of any experience with this. I know that Vector Red (another alkaline
phosphatase substrate) can be dehydrated through ethanol and the tissue
cleared and it is supposed to survive. One would think then that
embedding should also be possible. If epoxy gives a problem perhaps LR
white??

Richard Gardiner



From daemon Thu Jul 5 23:44:27 2001



From: colin.veitch-at-tft.csiro.au
Date: Fri, 6 Jul 2001 14:37:19 +1000
Subject: Xenosput Ti target

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

We have a Dynavac Xenosput Cr coater which has recently used up the last of
the Ti in the Ti sub pump target. When we ordered 2 replacement targets
(with part numbers specified) from a local dealer (I don't want to mention
company names) we received two pieces of metal (presumably Ti) which had
been cut from a sheet of metal using an oxy/acetylene torch. The pieces
were not finished in any way. Needless to say we were not overly
impressed!!

Does anyone know of sources (either local or international) for these
targets?

Many thanks for any information.

Colin Veitch

Instrumentation Scientist
Late Stage Innovation Group
CSIRO Textile and Fibre Technology
PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-tft.csiro.au
Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Fax: +61 (0) 3 5246 4811


The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may not
copy, distribute or take any action in reliance on it. If you have received
this message in error, please telephone CSIRO Textile and Fibre Technology
on +61 3 5246 4000.



From daemon Fri Jul 6 01:44:22 2001



From: Bharesh_Mandalia-at-gillette.com
Date: Fri, 6 Jul 2001 07:36:57 +0100
Subject: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
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Is there a way to convert greyscale SEM images to colour, like the ones at
the MicroAngelo website?

Many thanks,

Bharesh Mandalia




From daemon Fri Jul 6 03:02:27 2001



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Fri, 06 Jul 2001 09:55:57 +0200
Subject: Re: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
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Hi all

To contribute to the search for the roots of the Toluidine blue
procedure:

There is an interesting paper by Kramer & Windrum (J. Histochem
Cytochem, 1955, Vol 3, 227-237, The Metachromic Staining Reaction)
that probably was instrumental in initiating the wider use of Tol
Blue. In this paper the effect of stain concentration and residual
water is shown (amongst many other factors) to be important for the
development of the multiplicity of colours.

The use of Toluidine Blue as a stain for monitor sections of epoxy-
and acrylic-embedded tissue probably stems from these metachromic
effects that this stain produces, with the result that some deductions
regarding the chemical composition of the sample can be made after a
simple Tol Blue staining.

Even though we, in this lab, have since forever used Tol Blue for the
staining of monitor sections of resin-embedded tissues, every batch of
Tol Blue that we make up seems to have slightly different staining
characteristics. This was such a source of irritation that Chris van
der Merwe recently decided to systematically investigate some of the
variables.

He found that the most important parameters are:
1. Tol blue concentration (0.1 or 0.5%)
2. Buffer or vehicle type, pH and concentration (0.1 - 1%
Na-carbonate, Na-benzoate buffer at pH4, Na-tetraborate (borax) at pH
8.8)
3. Embedding resin formulation (Spurr, Quetol, Polarbed 812, LR-White)
4. Section thickness.

All the above have an effect on the intensity, as well as the degree
of metachromicity, of the stain.
Resin formulation is probably the major influencer of the results.

This may be a reason for the existence of the wide variety of Tol Blue
recipes that abound in the literature.

More information on Tol Blue staining can be had directly from Chris
at cvdmerwe-at-scienti.up.ac.za


Regards

Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm



"Monson, Frederick C." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Sally,
} To address the REAL attribution issue, one of us is going to have to
} go to a REALLY good library that has these OLD journals back to the late
} '50's. We may have to divide the labor, but the question is sufficiently
} interesting and meritorious that we ALL should get it right, when asked.
} Someone was responsible for the use of borax (Sodium tetraborate) with
} Toluidine blue O (1% in 1%). Here are all the citations that Pease provides
} and his [attributions]:
}
} 1. Trump, Schmuckler and Benditt (1961), J. Ultrastruc Res., 5:
} 343 [ 0.1% Toluidine blue O in 2.5% carbonate at pH 11 (confirmed in Lewis
} and Knight below)]
} 2. Charles and Meek , G.R. separately in a discussion of paper
} by Mercer(1963), J Microscp Sci., 81:179. [mentions 1% Toluidine blue O in
} 1% borax used at 85 degrees C]
} 3. Pease, D.C.(1964), Histological Techniques for Electron
} Microscopy (2d), Academic Press, NY, NY, pp 256-262. [mentions on p. 260 in
} 2st paragraph the exact method that I use for staining as 'his' method -
} "...flood the slide, cook until [edge] of drop [froths/]steams [or
} dries]....". [This is a long discussion to which Pease devotes an entire
} section, but one can just FEEL his impatience with the unimportance to HIM
} of this issue. The book, after all, is NOT about light microscopy. The
} semi-thin section is for orientation only, and hardly deserves any more
} notice - especially if the light microscopists cannot SEE what is to be seen
} in the best material ever handed them.]
} 4. In Kay, D.H.(ed)(1965), Techniques for electron
} Microscopy(2d), F.A. Davis, Philadelphia, ISBN: [NOT given - is this book
} pre-historic?!] The following is cited as THE reference for using Toluidine
} blue O: Benscome, Stone, Latta, and Madden (1959), J. biophys. biochem.
} cytol, 5: 508.
}
} I have just looked in Geoffrey Meek's book (1970), Practical
} Electron Microscopy for Biologists, Wiley_Interscience, NY, NY, p. 456-457.
} ISBN: 0-471-59030-4. 1% in 1% with no citation, though in this book he has
} purposely NOT used citations at all.
}
} I have also consulted the Sorvall manual on "Thin Sectioning"(3d
} Ed)(1973) as well as the SerVall manual "Thin Sectioning and Associated
} Technics for Electron Microscopy"(1959) which apparently was distributed
} with the MT-1. In the latter, only staining of Methacrylate sections was
} given and no mention of 1% *bor* of any kind. In the former, 1% borate at
} pH 4.5 is mentioned, but that is not relevant to this discussion.
}
} I would bet that you might have some of these references. If you do
} not, let me know, and I will dig them up, in old Philadelphia, in Ben
} Franklin's old stacks (at the U. of P(ennsylvania) as we call it). I will
} do it anyway, because I want to know, but I will do it quicker if you tell
} me I'm keeping you waiting.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}
} } ----------
} } From: Sally Stowe
} } Sent: Tuesday, July 3, 2001 3:37 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Tol blue/borax
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Somebody just asked me the reference for toluidine blue/sodium borate (1%
} } each) which is our routine LM stain - I cant find an attribution in any of
} } about 10 books. Can anybody help? This person should not be forgotten!
} } One suggestion was Alsop?
} }
} } thanks
} }
} } Sally
} }
} }
} }
} } Dr Sally Stowe
} } Facility Coordinator,
} } ANU Electron Microscopy Unit
} } Research School of Biological Sciences
} } Australian National University
} } Canberra ACT0200
} } AUSTRALIA
} } stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
} } ph 02 6125 2743
} } http://www.anu.edu.au/EMU
} }
} }
} }
} }
} }


From daemon Fri Jul 6 05:06:20 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Fri, 6 Jul 2001 20:03:50 +1000
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
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Continuing on this thread, I had always thought Richardson's stain was the
methylene blue/azure II combination, but not so, it seems, since the 1960
paper refers to both this stain and to tol blue. Someone emailed that
methylene blue must be used with azure II, but I don't think this is
necessary, though it may give better staining.

Re metachromicity, as Fred Monson and Jan Coetzee note, it depends quite a
bit on pH of the tol blue (for plant material at least), and used to depend
on the source of stain but I guess this is not such a problem these
days(?). We generally use a benzoate buffered solution of tol blue at pH
4.5 for fresh tissue and tissue embedded in hydrophilic resins like LR
White and other methacrylates, and a pH 10 solution (buffered, if
necessary, in sodium carbonate) for sections of hydrophobic epoxy resins -
e.g. Spurr's. Tol blue metachromicity isn't so good at high pH (at least
in my hands). The pH of tap water is usually slightly acidic, so aqueous
tol blue is generally OK for fresh tissue and methacrylate resins. Since
borate buffers are alkaline, perhaps this was the original reason they were
used for the hydrophobic resins(?) - and they do produce a much more
permanent stain.

Re. "old" references and books, most people here do molecular/genetic type
work and literature older than about 1995 is considered venerable..... I
am amazed (and appalled) at the great old histochemistry and cytochemistry
books thrown out by libraries - the source of most of my "old" books.

Friday night, burning a few CDs..... Looked up another reference on
histochemistry - A.G. Everson Pearse (1968) Histochemistry Vol 1 3rd edn
(SBN 7000-1326-1 for Fred Monson - perhaps ISBN came after this time....) -
a method from 1947 is given that uses a solution of 0.25% tol blue in 0.25%
borax followed by 0.5% potassium dichromate in sat'd mercuric chloride, for
permanent staining of paraffin sections prior to dehydration and mounting
in Canada Balsam. Hess M, Hollander F (1947) J Lab Clin Med 32:905

Mallory, whose name used to be associated with various staining protocols,
put it all together in a book: Mallory FB (1938) Pathological Technique.
WB Saunders, Philadelphia. This is where Mallory's borax methylene blue
comes from - 1% methylene blue plus 1% azure II in 1% borax. Lillie refers
to it in his book - Histopathologic Technic - we have the 2nd edition,
published in 1954, LC number 53-5622 - again, pre-ISBN.

Will finish now, in case this is getting too bor-ing!

cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Fri Jul 6 06:01:08 2001



From: Alan Stone :      as-at-astonmet.com
Date: Fri, 06 Jul 2001 05:56:00 -0500
Subject: EDS problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have a Link detector with AN1000 electronics and a Dapple Systems
analyzer. This has been a very stabile system for many years. I recently
lost count rate with a broad peak near zero. I ran a few cycles with my
conditioner and then was able to see ice at the bottom of the dewar (it has
been humid here). I proceeded to empty the dewar, dry it out with alcohol
and acetone and re-install.

I still have a low count rate and signal noise. Can anyone offer some
pointers or know of a local service engineer. I am in the Chicago area.

Alan Stone
ASTON


Alan Stone
ASTON Metallurgical Services


From daemon Fri Jul 6 07:32:56 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 6 Jul 2001 08:24:15 -0400
Subject: RE: bcip/NBT substrate and resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hank,
There are lots of books that explain the histochemical methods for
alkaline phosphatase, but few that concentrate on EM. NBT is converted to a
formazan that is insoluble, but not electron dense. I'm not certain about
the effect of OsO4 on the formazan but it has been used to osmicate the ppt
when DAB is used. There is no need to go further here, except that
phosphatase histochemistry at the EM level has been accomplished by using
metal capture methods for some time.

I recommend a book that has the information you need beyond what I
have given above. One with protocols is:

Ogawa, K. and Barka, T.(1993), Electron Microscopic Cytochemistry
and Immunocytochemistry in Biomedicine, CRC Press, Boca Raton, FL, ( for the
ILL reprint: pp. 140-144("Alkaline Phosphatase")), ISBN: 0-8493-6012-9, LC
Card #: 92-9241, LC RB46.7.E43 (In the library). In truth, this book
should be in the library at Baylor. If not, perhaps you might get it
ordered. It is not out of date. Most of the recent (since '80's)
innovations in enzyme histochemistry have concerned buffering and fixing to
retain maximum enzymatic activity while not sacrificing structural
integrity. In addition, as in LM, you will need to use controls for the EM
specimens.

You might also want to consider something like the following
protocol/approach.

URL:
http://www.scanning-fams.org/scanabstracts/SCANNING96/18259.html

Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.








} ----------
} From: hpadams
} Sent: Thursday, July 5, 2001 4:10 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: bcip/NBT substrate and resin
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have experience with embedding, in resin, tissue fixed in 4%PF
}
} that was labelled with an alkaline phospatase secondary ab reacted with
} the
} substrate BCIF/NBT. The concern is the fading/dissolving/diffusion of the
} substrate during processing.
}
} Hank Adams
} Manager
} Integrated Microscopy Core
} Molecular and Cellular Biology
} Baylor College of Medicine
} Houston, Tx 77030
}
}
}


From daemon Fri Jul 6 08:17:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 6 Jul 2001 09:12:14 -0400
Subject: RE: Modifying a Prism with a slight chide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Damian,
Seems to me you have gotten to the nexus of the problem. Which
optical path do you want? Straight ahead or 45 degrees. If straight ahead,
store the part and cover the opening. If 45 degrees then spend a few
dollars (i.e., http://www.mwkindustries.com/A-f/miru1.htm + a 45degree block
and rod) so you can get all 100%. Then, when the current project is over,
you can put the scope back together so someone else can use the 80/20. I'm
assuming, from what you say that you don't want to use the oculars at all -
ever.

Regards,

Fred Monson


} ----------
} From: Damian
} Sent: Thursday, July 5, 2001 5:24 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Modifying a Prism
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I wrote about modifying a Nikon Diaphot 80/20 prism and received some good
}
} suggestions (the 100/0) prism is no longer available from Nikon). When I
} took the cover off the side of the microscope and removed the prism, I
} discovered that one solution might be simply covering the binocular output
}
} surface of the 80/20 prism. Would there be a problem with this
} approach? I realize that I would only have 80% going to the specimen but
} that may be tolerable. What would be the best way to block the laser beam
}
} from exiting from the 20% prism surface? Black tape? Black paint? Black
}
} metal ? Silver tape covered with black tape?
}
} Thanks for the help
}
} Damian
}
}
}


From daemon Fri Jul 6 09:35:27 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 7 Jul 2001 00:30:14 +1000
Subject: RE: Xenosput Ti target

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ProSciTech was not THAT supplier. We have excellent suppliers for targets,
including Ti targets - I have no idea about the Xenosput target dimensions.
Targets seem to be a simple product but there are great price and quality
variations, yet high price frequently does not result in high quality.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, July 06, 2001 2:37 PM,
"colin.veitch-at-tft.csiro.au"-at-sparc5.microscopy.com
[SMTP:"colin.veitch-at-tft.csiro.au"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
}
} We have a Dynavac Xenosput Cr coater which has recently used up the last of
} the Ti in the Ti sub pump target. When we ordered 2 replacement targets
} (with part numbers specified) from a local dealer (I don't want to mention
} company names) we received two pieces of metal (presumably Ti) which had
} been cut from a sheet of metal using an oxy/acetylene torch. The pieces
} were not finished in any way. Needless to say we were not overly
} impressed!!
}
} Does anyone know of sources (either local or international) for these
} targets?
}
} Many thanks for any information.
}
} Colin Veitch
}
} Instrumentation Scientist
} Late Stage Innovation Group
} CSIRO Textile and Fibre Technology
} PO Box 21, BELMONT, Vic. 3216. Australia.
}
} E-mail: colin.veitch-at-tft.csiro.au
} Web: http://www.tft.csiro.au
}
} Tel: +61 (0) 3 5246 4000
} Fax: +61 (0) 3 5246 4811
}
}
} The information contained in this e-mail message may be privileged or
} confidential information. If you are not an intended recipient, you may not
} copy, distribute or take any action in reliance on it. If you have received
} this message in error, please telephone CSIRO Textile and Fibre Technology
} on +61 3 5246 4000.



From daemon Fri Jul 6 10:28:16 2001



From: Glenn Holm :      gholm-at-mit.edu
Date: Fri, 06 Jul 2001 11:19:33 -0400
Subject: Re: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If they are in a format such as TIFF that can be opened with a picture
editing programs like Photoshop (including Photoshop LE) or NIH image or
Paintshop Pro or probably others, just change the color mode to indexed
color and apply appropriate color palette or LUT - they come with samples
palettes. Paintshop Pro has the advantage of using ASCII format palettes
which can be made in Excel (Red, Green Blue values 0-255) and imported.


} From: "Bharesh_Mandalia-at-gillette.com"-at-sparc5.microscopy.com
} Subject: False Colour SEM Images
} To: Microscopy-at-sparc5.microscopy.com
} X-Mailer: Lotus Notes Release 5.0.6 December 14, 2000
} Date: Fri, 6 Jul 2001 07:36:57 +0100
} X-MIMETrack: Serialize by Router on GBO827N/BO/Gillette(Release 5.0.5
} |September 22, 2000) at
} 07/06/2001 02:34:29 AM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

-----------------------------------------------------------
Glenn Holm {mailto:gholm-at-mit.edu}
Graybiel Lab (617)253-5780;fax (617)253-1599
M.I.T Dept. of Brain + Cog. Sci. Cambridge, MA 02139
----------------------------------------------------------



From daemon Fri Jul 6 11:52:12 2001



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Fri, 6 Jul 2001 11:26:39 -0500
Subject: Brightness/Contrast Calibration Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists,

We're looking for suggestions for SEM standards to calibrate
brightness/contrast settings (for example, setting an auto B/C feature in
the SEM software, so that the desired repeatable B/C level is obtained by
multiple users). Our customers' goal is to do correction/matching of gray
scale histograms between images of different samples, and they would like to
make the process of adjusting B/C levels at the start of image acquisition
to be as standardized as possible. The imaging might be done in secondary
or backscattered mode, so suggestions for both would be appreciated.

Thanks vey much for you input,

Elaine

Elaine F. Schumacher
Analytical Specialist
UOP LLC
25 East Algonquin Road
Des Plaines, IL 60017-5017
Phone: 847-391-3403
Fax: 847-391-3719
Email: efschuma-at-uop.com



From daemon Fri Jul 6 12:16:03 2001



From: pjp6 :      pjp6-at-dana.ucc.nau.edu
Date: Fri, 06 Jul 2001 10:11:17 -0700
Subject: FWD: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Adobe photoshop is an excellent application for manipulating both SEM and TEM
images. Conversion of gray scale images to color requires a little practice
with the software but the results are well worth the effort. I have also used
Adobes Illustrator to design and produce presentation posters of my images.

Pete Polsgrove
pjp6-at-dana.ucc.nau.edu









} ===== Original Message From
"Bharesh_Mandalia-at-gillette.com"-at-sparc5.microscopy.com =====
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Is there a way to convert greyscale SEM images to colour, like the ones at
the MicroAngelo website?

Many thanks,

Bharesh Mandalia




From daemon Fri Jul 6 13:30:50 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 6 Jul 2001 08:24:01 -1000 (HST)
Subject: Re: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Bharesh-

} Is there a way to convert greyscale SEM images to colour, like the ones at
} the MicroAngelo website?

I personally "paint" each SEM image with Photoshop, rather than use lookup
tables. For TEMs, though, I sometimes use LUTs.

I have a graphics tablet and stylus and I enlarge each image so that I can
practically see each pixel and I apply color with the paintbrush in Color
mode, so this is a slightly more "artistic" endeavor, rather like
paint-by-numbers! It takes hours and hours for each image. However, I have
seen some great results with applying LUTs, which is certainly a lot
faster!

Experiment. Have fun!

Aloha,
Tina (MicroAngela)

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Fri Jul 6 14:30:17 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 06 Jul 2001 12:22:50 -0700
Subject: Re: EDS problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Alan,
It is probably worth getting your detector serviced. My experience with ice
in the dewar was that I had to warm it up thoroughly to get rid of ice that
had condensed inside the crystal/preamp part, where you can't get at it with
solvents and then the moisture in the vacuum caused poor holding time for
the LN2, so I had the dewar pumped. Jim Nicolino of AAT
(JNicolino-at-xraydetectors.com), which services all makes of detectors, says
it is worth getting the whole thing properly, professionally checked out,
the molecular sieve replaced and the detector brought back up to factory
specs. He is probably right.
I have no affiliation with AAT except as a satisfied customer.
At 05:56 AM 7/6/01 -0500, you wrote:
}
} I have a Link detector with AN1000 electronics and a Dapple Systems
} analyzer. This has been a very stabile system for many years. I recently
} lost count rate with a broad peak near zero. I ran a few cycles with my
} conditioner and then was able to see ice at the bottom of the dewar (it has
} been humid here). I proceeded to empty the dewar, dry it out with alcohol
} and acetone and re-install.
}
} I still have a low count rate and signal noise. Can anyone offer some
} pointers or know of a local service engineer. I am in the Chicago area.
}
} Alan Stone
} ASTON Metallurgical Services
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Jul 6 15:44:11 2001



From: coviello :      coviello-at-mae.uta.edu
Date: Fri, 06 Jul 2001 03:33:27 -0500
Subject: em-print matching software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
I was told that there exists image matching software that allows one to
calibrate the image one wants for the specific paper one is printing on.

Has anyone used this type of software and if so, have any
recommendations
The less expensive, the better?
Thanks,
Mike Coviello
UTA



From daemon Fri Jul 6 17:46:26 2001



From: Smartech :      smartech-at-optonline.net
Date: Fri, 06 Jul 2001 18:46:33 -0400
Subject: A comparison of the ability to detect light elements using an EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anybody know of a good article that compares EDS detectors, from a
variety of manufacturers, w/ respect to their light element sensitivity?

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Fri Jul 6 18:25:22 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 6 Jul 2001 17:09:16 -0600
Subject: Re: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unfortunately it may not be as easy as that. If you change the LUT, the
changes are applied to all pixels of the image. If your object has similar
gray values than other parts of the image, you end up with colors all over
the image.

You basically have to segment the image first into parts that you want to
change and parts you want to stay untouched, then color one part (for
example using the LUT functions), then put the two parts together again.

If you want to get some recipes on how to do this, you might want to ask Dr.
Gary Gaugler about it. He has used our software (analySIS) and other to the
kind of coloring you are interested in. Go to www.photoweb.net to find out
more.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Glenn Holm [mailto:gholm-at-mit.edu]
Sent: Friday, July 06, 2001 9:20 AM
To: Microscopy-at-sparc5.microscopy.com


If they are in a format such as TIFF that can be opened with a picture
editing programs like Photoshop (including Photoshop LE) or NIH image or
Paintshop Pro or probably others, just change the color mode to indexed
color and apply appropriate color palette or LUT - they come with samples
palettes. Paintshop Pro has the advantage of using ASCII format palettes
which can be made in Excel (Red, Green Blue values 0-255) and imported.


} From: "Bharesh_Mandalia-at-gillette.com"-at-sparc5.microscopy.com
} Subject: False Colour SEM Images
} To: Microscopy-at-sparc5.microscopy.com
} X-Mailer: Lotus Notes Release 5.0.6 December 14, 2000
} Date: Fri, 6 Jul 2001 07:36:57 +0100
} X-MIMETrack: Serialize by Router on GBO827N/BO/Gillette(Release 5.0.5
} |September 22, 2000) at
} 07/06/2001 02:34:29 AM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

-----------------------------------------------------------
Glenn Holm {mailto:gholm-at-mit.edu}
Graybiel Lab (617)253-5780;fax (617)253-1599
M.I.T Dept. of Brain + Cog. Sci. Cambridge, MA 02139
----------------------------------------------------------



From daemon Fri Jul 6 18:25:44 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 06 Jul 2001 16:18:12 -0700
Subject: HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Liststers!
I would appreciate any information on the safety of hexamethyldisilazane (HMDS). I didn't get much out of the data sheet. I would like to know what I am working with... Is it hazardous to one's health? I don't have a hood in my room, but I could move to another room which does have a hood. Thanks!





From daemon Fri Jul 6 21:15:08 2001



From: Maria Lada :      m.lada-at-sheffield.ac.uk
Date: Fri, 6 Jul 2001 21:44:27 -0500
Subject: Software for crystal structure/TEM simulation for PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We always use HMDS in a hood. I'm home and info is at work though.

Ron
----- Original Message -----
} From: "Barbara Plowman" {Bplowman-at-sfmail.dental.uop.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, July 06, 2001 7:18 PM


Dear ALL,

Is there a version of MAcTempas/CrystalKit for a Windows/PC platform?

Is there nay other windows platform Package doing a similat job?

with thanks

Maria Lada
EEE department
University of Sheffield


From daemon Sat Jul 7 12:18:41 2001



From: Gordon Nord :      gnord-at-mindspring.com
Date: Sat, 07 Jul 2001 13:05:52 -0400
Subject: Re: Software for crystal structure/TEM simulation for PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Maria,

I don't think there is a PC version of MacTempas.

The nearest program in the PC world is JEMS.
I recently examined what was available from PC's particularly a program
that could simulate electron diffraction.

I purchased Pierre Stadelman's JEMS - runs in JAVA - and it is beautiful
for both images and diffraction.
Runs very nicely on a Compaq 1.6 GHz machine with 512 MB and will also run
on a Mac or Sun.
I have already set up all the amphibole minerals mainly for electron
diffraction work.
Very useful so far. JEMS also plays well with triclinic phases something
many other programs have difficulty with.
Something to do with right angles I guess.

Check it out at {http://cimewww.epfl.ch/people/stadelmann}

MacTempas and CrystalKit together were(?) unique however in how they were
able to treat interfaces such as twins.
I don't think JEMS does this very easily.

I found however that if you are interested in looking at crystal structures
neither CrystalKit nor JEMS can compete with David Palmer's CrystalMaker
program which currently runs only on a Mac. He is however working on a C++
version that will run on PC's.

Check it out at {http://www.crystalmaker.co.uk} and email him that you are
anxiously awaiting the PC version.

My advice is purchase an $800 iMac and run the Mac programs and give JEMS a
good look for your PC.
Can't have too many platforms.

Cheers,
Gordon Nord
US Geological Survey - Emeritus


Maria Lada wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear ALL,
}
} Is there a version of MAcTempas/CrystalKit for a Windows/PC platform?
}
} Is there nay other windows platform Package doing a similat job?
}
} with thanks
}
} Maria Lada
} EEE department
} University of Sheffield





From daemon Sun Jul 8 10:50:32 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 8 Jul 2001 10:29:11 -0600
Subject: Re: HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HMDS *MUST* be used in a hood. I'm at home as well, so don't have the
MSDS, but there is no question about this.

Phil

} Hi Liststers!
} I would appreciate any information on the safety of
} hexamethyldisilazane (HMDS). I didn't get much out of the data
} sheet. I would like to know what I am working with... Is it
} hazardous to one's health? I don't have a hood in my room, but I
} could move to another room which does have a hood. Thanks!

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sun Jul 8 13:52:42 2001



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Mon, 9 Jul 2001 08:35:55 +0100
Subject: HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Maria,
Thierry Epicier has written SIMPLY-S code for HREM image simulation (and
more) for the PC. The simulation part is based on the original SHRLI
(simulated high-resolution lattice image) code. Since MacTempas is a
port to the Mac of the TEMPaS code (developed from SHRLI to run on a Vax
system with a Tektronics GUI), the results of SIMPLY-S and MacTempas
should be the same.
You can reach Thierry at Thierry.Epicier-at-insa-lyon.fr
Mike O'Keefe

----- Original Message -----
} From: "Maria Lada" {m.lada-at-sheffield.ac.uk}


Hi Barbara,

I received my HMDS from Agar Scientific and it came with a 6 page
material safety data sheet.

In short:

it is highly flammable, toxic, harmful by inhalation, in contact with
skin and if swallowed, irritating to eyes, causes severe skin irritation,
is readily absorbed through the skin - and the target organ is nerves.

In case of contact with eye, rinse immediately with plenty of water
and seek medical advice. Wear suitable protective clothing.

You find MSDS in the internet by doing a keyword search and the
major EM suppliers have MSDS of their products on their web sites.

I always use it in the fume cupboard, with gloves - and make sure
that their is no hot plate near by. (in fact I handle it as I would
handle Propylen oxide).

Regards

Claudia

Date sent: Fri, 06 Jul 2001 16:18:12 -0700
} From: Barbara Plowman {Bplowman-at-sfmail.dental.uop.edu}
To: Microscopy {Microscopy-at-sparc5.microscopy.com}


Hi Liststers!
I would appreciate any information on the safety of hexamethyldisilazane (HMDS). I didn't get much out of the data sheet. I would like to know what I am working with... Is it hazardous to one's health? I don't have a hood in my room, but I could move to another room which does have a hood.
Thanks!




Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk
Fax: 44(0)208 547 7562


From daemon Mon Jul 9 04:14:17 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Mon, 09 Jul 2001 09:48:43 +0000 (GMT)
Subject: Re: False Colour SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Baresh,
there is a very easy, quick and effective way if you have Adobe PhotoShop:

1) Create a new layer above your image.
2) Select the region you want to colour.
3) Fill it with the colour you want.
4) Change the layer properties from 'Normal' to Overlay. Done!

You may do step (2) by drawing round the region with the 'freehand select' or 'polygon select' tool, or on the lower layer using the magic wand.

Good luck,

Richard

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street London
W1J 6AQ





From daemon Mon Jul 9 07:33:19 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 9 Jul 2001 07:18:45 -0500
Subject: RE: Brightness/Contrast Calibration Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Elaine,

A BSE standard can be made from almost any materials (suitable for SEM).
The std should be ground and polished to remove topography contrast. What
materials you choose will depend on the examination goals.

This brings me to a point. I would not stress BSE "calibration" as a
generally useful technique. Under VERY limited circumstances, such
calibration/standardization can be beneficial. Routine use of this
technique will guarantee that sooner or later an important phase will be
missed when examining an unknown. When examining with BSE, I find it
imperative to be "all over the map" with the BSE B&C to be sure I am not
missing something. It is not unheard of to need two or more images to
define what is happening. Sometimes there is simply not enough dynamic
range presented in one image to perceive all variations in composition.

Regards,
Woody White, McDermott Technology, Inc.

} -----Original Message-----
} From: Schumacher, Elaine [mailto:efschuma-at-uop.com]
} Sent: Friday, July 06, 2001 12:27 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Brightness/Contrast Calibration Standards
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Fellow Microscopists,
}
} We're looking for suggestions for SEM standards to calibrate
} brightness/contrast settings (for example, setting an auto
} B/C feature in
} the SEM software, so that the desired repeatable B/C level is
} obtained by
} multiple users). Our customers' goal is to do
} correction/matching of gray
} scale histograms between images of different samples, and
} they would like to
} make the process of adjusting B/C levels at the start of
} image acquisition
} to be as standardized as possible. The imaging might be done
} in secondary
} or backscattered mode, so suggestions for both would be appreciated.
}
} Thanks vey much for you input,
}
} Elaine
}
} Elaine F. Schumacher
} Analytical Specialist
} UOP LLC
} 25 East Algonquin Road
} Des Plaines, IL 60017-5017
} Phone: 847-391-3403
} Fax: 847-391-3719
} Email: efschuma-at-uop.com
}
}


From daemon Mon Jul 9 08:19:29 2001



From: Jim Nicolino :      JNicolino-at-xraydetectors.com
Date: Mon, 09 Jul 2001 09:15:49 -0400
Subject: EDS Sensitivity for Light Elements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Smartech,
If someone published such an article comparing EDS detectors as to their
ability to detect light elements it should be the SEM manufacturers. The
light element sensitivity is determined by the geometry of the detector
not the entrance window. Basically the higher the take-off angle of the
emerging photon, the better the sensitivity due to the absorption of the
matrix. The high voltage of the electron beam also changes the
sensitivity due to the depth of penetration and therefore the depth of
the x-ray source.
Operating a EDX repair facility, I work on all of the EDX manufacturer
detectors. With one possible exception, all the manufacturers use the
same entrance window material, using various trade mark names. There
also seems to be very little difference is the Si(Li) crystals or FET's
which are the two internal components of the detectors. Since all of the
manufacturers use a resolution specification for Fe55 photons and a peak
to background ratio for the 1.0 Kev channel, there would be no
comparison specifications for light element sensitivity.
I hope this answers your question.
Jim Nicolino


From daemon Mon Jul 9 08:46:24 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 09 Jul 2001 08:40:42 -0500
Subject: RE: Brightness/Contrast Calibration Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I concur with Woody's comments, and offer a couple of more.

It is often possible for the same sample type to set BSE brightness to be
consistent between images, and there are many time you would want to do
that. We do image analysis of concrete and use calcite and graphite to
repeatably set our signal. Some images look good that way, but even then,
variation in polishing can greatly affect what the final image looks like.
Sometimes it is not so good.

Last week I was looking at samples of a Cr-Al alloy. (It required much
different contrast than did the concrete.) Some areas of some samples had
three phases present (Al, Al-Cr, and Cr); others had only two (Al and
Al-Cr). My inclination was naturally to first turn up the contrast to
spread the signal throughout the grayscale to show the character of what
was present. I later went back to take some images at consistent signal
levels to show that the Cr-rich phase was missing.

Now "calibration" is a reasonable process for BSE. It is much less
tractable for SE. SE signals are just not that consistent. Since they are
so sensitive to topography, I find myself changing contrast all the time. I
expect to need to reduce contrast as I increase magnification as more
details come into view. That is the nature of it.

I wonder, do you have a waveform monitor on your scope to help in setting
contrast? We use ours religiously and I have been surprised to hear that
some scopes don't have them. (I guess the one we had on our JEOL U3 was an
add-on, but since they have been part of our spec.) We have determined
where white and black are on our waveform display, and before each photo we
check the waveform to be sure that the signal is within our recordable range.

There is still some experience involved in setting the level so the picture
looks good afterwards. A picture may be completely within the recordable
range and still "look" too dark or light. I tend to prefer my background
away from full black or white even if there is nothing to see in it. That
just takes practice since there is such a wide variety of samples out there.

Digital imaging with gamma control after the fact has done a lot to make
sure the first picture will be a keeper. If you don't have that yet, please
consider it, or figure that your operators will just have to practice more.

Cheers,
Warren

At 07:18 AM 7/9/2001 -0500, you wrote:
} Elaine,
}
} A BSE standard can be made from almost any materials (suitable for SEM).
} The std should be ground and polished to remove topography contrast. What
} materials you choose will depend on the examination goals.
}
} This brings me to a point. I would not stress BSE "calibration" as a
} generally useful technique. Under VERY limited circumstances, such
} calibration/standardization can be beneficial. Routine use of this
} technique will guarantee that sooner or later an important phase will be
} missed when examining an unknown. When examining with BSE, I find it
} imperative to be "all over the map" with the BSE B&C to be sure I am not
} missing something. It is not unheard of to need two or more images to
} define what is happening. Sometimes there is simply not enough dynamic
} range presented in one image to perceive all variations in composition.
}
} Regards,
} Woody White, McDermott Technology, Inc.
}
} } -----Original Message-----
} } From: Schumacher, Elaine [mailto:efschuma-at-uop.com]
} } Sent: Friday, July 06, 2001 12:27 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Brightness/Contrast Calibration Standards
} }
} } Fellow Microscopists,
} }
} } We're looking for suggestions for SEM standards to calibrate
} } brightness/contrast settings (for example, setting an auto
} } B/C feature in
} } the SEM software, so that the desired repeatable B/C level is
} } obtained by
} } multiple users). Our customers' goal is to do
} } correction/matching of gray
} } scale histograms between images of different samples, and
} } they would like to
} } make the process of adjusting B/C levels at the start of
} } image acquisition
} } to be as standardized as possible. The imaging might be done
} } in secondary
} } or backscattered mode, so suggestions for both would be appreciated.
} }
} } Thanks vey much for you input,
} }
} } Elaine
} }
} } Elaine F. Schumacher
} } Analytical Specialist
} } UOP LLC
----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Jul 9 08:49:37 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Mon, 9 Jul 2001 09:45:42 -0400
Subject: Re: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The main tricks are to low-angle mill both sides of the specimen and to not
use Cu grids. We switched to Mo grids years ago and we rarely see back
sputtered material on samples milled on both sides (from the substrate
direction, of course). If you want to use up your stock of Cu grids, cut
the back side of the grid away so that there is no grid material beyond the
specimen to sputter from. Grid becomes the "C" shaped grid used for FIB
prepped specimens. Sometimes some sputtered material persists after very
low angle milling. In that case, pop the sample into a conventional
high-angle mill and mill at 25 degrees for 30 seconds at 2 or 3 KeV to
clean off the sputter artifact.



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Richard Beanland +44 1327 356363 {richard.beanland-at-marconi.com} on
07/02/2001 07:02:41 AM

To: Feng Wu {fwu-at-bgumail.bgu.ac.il}
cc: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}


Feng,
the trick is not to have a hole in your specimen where the ions can go
through and back-sputter the Cu on to your sample. So make your specimen
big enough to cover up the hole in the support grid and stop ion milling
immediately when a hole appears. The problem will go away to some extent
if you use the two-sided specimen holder. Alternatively, use an inert grid
and dissolve the Cu with an acid after specimen prep. (Most acids are
unlikely to affect GaN or sapphire.)

Richard




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} I met some problems during I prepared the samples for GaN/sapphire
plan-view
} using tripod wedge technique. After polishing, the sample was thinned by
pips
} in one side. However, The other side usually was covered by copper, as a
result
} the sample became dark. It seems the unthinned side should be covered
something
} to prevent sputtered materials from growing on it. Do you have some
knowledge
} on it?
}
} Thanks in advance.
}
} Best regards.
}
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
} fax 972-8-6472944
} fwu-at-bgumail.bgu.ac.il
} **********************************************
}
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street
London
W1J 6AQ










From daemon Mon Jul 9 09:06:05 2001



From: Gary Gill :      garygill-at-dcla.com
Date: Mon, 9 Jul 2001 08:58:54 -0500
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another potential cause of variation is dye concentration. To be certified
by the Biological Stain Commission, toluidine blue dye lots must have at
least 50% dye content. Unless corrected each time, toluidine blue solutions
will vary in dye content.

Gary Gill

-----Original Message-----
} From: Rosemary White [mailto:Rosemary.White-at-pi.csiro.au]
Sent: Friday, July 06, 2001 5:04 AM
To: microscopy-at-sparc5.microscopy.com


Continuing on this thread, I had always thought Richardson's stain was the
methylene blue/azure II combination, but not so, it seems, since the 1960
paper refers to both this stain and to tol blue. Someone emailed that
methylene blue must be used with azure II, but I don't think this is
necessary, though it may give better staining.

Re metachromicity, as Fred Monson and Jan Coetzee note, it depends quite a
bit on pH of the tol blue (for plant material at least), and used to depend
on the source of stain but I guess this is not such a problem these
days(?). We generally use a benzoate buffered solution of tol blue at pH
4.5 for fresh tissue and tissue embedded in hydrophilic resins like LR
White and other methacrylates, and a pH 10 solution (buffered, if
necessary, in sodium carbonate) for sections of hydrophobic epoxy resins -
e.g. Spurr's. Tol blue metachromicity isn't so good at high pH (at least
in my hands). The pH of tap water is usually slightly acidic, so aqueous
tol blue is generally OK for fresh tissue and methacrylate resins. Since
borate buffers are alkaline, perhaps this was the original reason they were
used for the hydrophobic resins(?) - and they do produce a much more
permanent stain.

Re. "old" references and books, most people here do molecular/genetic type
work and literature older than about 1995 is considered venerable..... I
am amazed (and appalled) at the great old histochemistry and cytochemistry
books thrown out by libraries - the source of most of my "old" books.

Friday night, burning a few CDs..... Looked up another reference on
histochemistry - A.G. Everson Pearse (1968) Histochemistry Vol 1 3rd edn
(SBN 7000-1326-1 for Fred Monson - perhaps ISBN came after this time....) -
a method from 1947 is given that uses a solution of 0.25% tol blue in 0.25%
borax followed by 0.5% potassium dichromate in sat'd mercuric chloride, for
permanent staining of paraffin sections prior to dehydration and mounting
in Canada Balsam. Hess M, Hollander F (1947) J Lab Clin Med 32:905

Mallory, whose name used to be associated with various staining protocols,
put it all together in a book: Mallory FB (1938) Pathological Technique.
WB Saunders, Philadelphia. This is where Mallory's borax methylene blue
comes from - 1% methylene blue plus 1% azure II in 1% borax. Lillie refers
to it in his book - Histopathologic Technic - we have the 2nd edition,
published in 1954, LC number 53-5622 - again, pre-ISBN.

Will finish now, in case this is getting too bor-ing!

cheers,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Mon Jul 9 09:47:29 2001



From: zaluzec-at-microscopy.com
Date: Mon, 9 Jul 2001 09:43:18 -0500
Subject: Re: EDS Sensitivity IMHO Windows do make a difference!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Smartech

I must disagree with Jim N. abit.

Yes geometry, accelerating voltage,
sample composition and thickness have a huge effect,
but you have to compare things correctly. Start
with the detector and its window, if it isn't configured properly
to start with then the sample and operating conditions
can be irrelevant.

There are different windows out there, and not all windows are identical
even if they are made from the same nominal material.
Variation in thickness of the different components
can make a significant difference in the performance of a window.
This was shown in a round robin test run by Larry Thomas a long
while back

Comparison of UTW/WL Detectors....
L.E. Thomas etal
Analytical Electron Microscopy - 1984
San Francisco Press. pge 333-336.

It is true that the windows are more uniform today than they
were back then, but there is still variation. In addition
any contamination deposited on the window by the instrument
environment (or ice formation inside the cryostat), and
of course electronic noise will significantly
deteriorate a detectors low energy performance.


If you wish to see a plot of detection efficiency vs detector window
type (which DOES make a difference if the windows are different)
lookup the following reference

N.J. Zaluzec ,
Evolutionary Developments in X-ray and Electron Energy Loss
Microanalysis Instrumentation ....
MicroBeam Analysis, 1990
San Francisco Press. pge 330-333.


Some of the figures from these two papers can also be found in this
PDF documnet

http://tpm.amc.anl.gov/Lectures/XEDS-Apr2001.pdf


My 2 cents....

Nestor
Your Friendly Neighborhood SysOp


From daemon Mon Jul 9 10:07:32 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Mon, 09 Jul 2001 10:59:06 -0400
Subject: adsorbtion problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
My protein sample does not adsorb to the grid even after glow
discharg, it is a membrane protein complex with the membrane lipids
froming vescicles. Nothing show up after negative staining.
What should I do to improve the adsorption? I have tried quick dring
with filter paper after put the sample on the grid, but it does not
work.

Thank you



From daemon Mon Jul 9 10:22:03 2001



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 09 Jul 2001 10:59:27 -0400
Subject: Amray 1200 - Anyone need one? II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sealed bid sale,

perfect microscope for home or garage or for any small lab needind an
addition al imaging microscope. This scope is in great shape and needs
a home with someone who can appreciate the fantastic imaging
capabilities and the user serviceable nature of the scope!

Please check the Central Michigan University Purchasing Department Web
site for details:
http://www.purchasing.cmich.edu/frametemp/sales_main.html
(go to the sealed bid items under "Pop Stops" or check the link below)

http://www.purchasing.cmich.edu/scripts/sale_bidinventory.pl

Specific questions about the microscope can be directed at me, all other

term of sale questions should be brought up with purchasing.

Thank you

Geoff Williams
Microscopy Specialist
Biology Department
Central Michigan University
Mt. Pleasant, MI 48859
989 774 3576 (voice)
989 774 3462 (fax)







From daemon Mon Jul 9 11:58:58 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 09 Jul 2001 09:04:34 -0500
Subject: Re: A comparison of the ability to detect light elements using

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor Zaluzec has a PPT presentation about EDS on the Argonne National Lab
website that he advertised a few weeks back.

Part of that presentation deals with sensitivities for the various window
materials and detector types. There are significant differences. No one
detector is best for all situations and all elements. You may have to
determine which detector is best for your combination of elements.

There should be similar critiques available through any number of sources,
but they will probably have to be a general discussion. Manufacturers have
a way of changing things and there may be something new, or at least
slightly different on the market this week.

Warren

At 06:46 PM 7/6/2001 -0400, you wrote:

} Does anybody know of a good article that compares EDS detectors, from a
} variety of manufacturers, w/ respect to their light element sensitivity?
}
} SMARTech
} 860-491-3299
} www.semguy.com
} 19 Cornwall Drive
} Goshen CT 06756

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Jul 9 11:59:00 2001



From: wft03-at-health.state.ny.us
Date: Mon, 9 Jul 2001 12:54:32 -0400
Subject: Re: adsorbtion problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Hello,
My protein sample does not adsorb to the grid even after glow
discharg, it is a membrane protein complex with the membrane lipids
froming vescicles. Nothing show up after negative staining.
What should I do to improve the adsorption? I have tried quick dring
with filter paper after put the sample on the grid, but it does not
work.

Dear Mike,
Unfortunately, the appropriate conditions for optimal coverage vary
from sample to sample. You can change the hydrophobicity/hydrophyllicity
of a thin carbon layer by "aging" over a few days for more hydrophobic
carbon, glow discharging for more hydrophyllic carbon, and glow discharging
in the presence of a volatile amine (we have amyl amine) for even more
hydrophyllicity. You can also use either the side of the thin carbon--if,
indeed, that is your substrate--which was in contact with mica--if the
carbon was vacuum-evaporated onto freshly-cleaved mica--or the side away
from the mica. Each of these has somewhat different properties. If you
are using carbon-coated formvar as a substrate, you may still be able to
alter its properties, but not in as many ways as with a thin carbon film.
You do not say whether you are testing a preparation for cryo-EM by
looking at a negatively-stained grid or whether you just want to look at a
neg-st specimen. If the former, try blotting from one side only. Blotting
from the back should leave more of the vesicles on the grid (although the
thickness of the specimen when frozen can be hard to control). Also, you
need to assure yourself that you can identify your specimen after mock cryo
preparation followed by warming up and staining; it may look very little
like a typical neg-st prep. If the latter, try letting the specimen with
the stain sit on the grid for several minutes before blotting.
This kind of preparation can be very tricky, and you just need to try
different combinations of conditions until you get good coverage. Good
luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Jul 9 13:25:19 2001



From: giselle melville :      giselledgt-at-excite.com
Date: Mon, 9 Jul 2001 11:18:22 -0700 (PDT)
Subject: unsubscibe

Contents Retrieved from Microscopy Listserver Archives
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Please take me off of the mailing list asap.

I would appreciate it.





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Mon Jul 9 14:10:14 2001



From: William McManus :      billemac-at-biology.usu.edu
Date: Mon, 9 Jul 2001 13:04:32 -0600
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To Sally and all:

I guess that my original post was too curt. We have gone away from Tol
Blue, to methylene blue due to safety reasons. There is no toxicology
data on the MSDS for Tol Blue, but there is for Meth Blue. As I have a
pregnant technician, I feel better knowing the LD 50 information, which
is on the MSDS for Meth Blue. But, methylene blue is not as good for a
general stain as Tol Blue, so I was just curious if there was an
overriding reason to use it. I am presently experimenting with food
based stains, which eliminates the issue completely.

The issue of pregnant (or possibly pregnant) workers in the lab has
turned out to be fairly complicated. If anyone else out there has beem
through this situation, I would like to know how you dealt with it.

Bill

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920

-----Original Message-----
} From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
Sent: Thursday, July 05, 2001 10:20 AM
To: 'Sally Stowe'
Cc: 'Microscopy Listserver'


Dear Sally,
To address the REAL attribution issue, one of us is going to
have to
go to a REALLY good library that has these OLD journals back to the
late
'50's. We may have to divide the labor, but the question is
sufficiently
interesting and meritorious that we ALL should get it right, when asked.
Someone was responsible for the use of borax (Sodium tetraborate) with
Toluidine blue O (1% in 1%). Here are all the citations that Pease
provides
and his [attributions]:

1. Trump, Schmuckler and Benditt (1961), J. Ultrastruc
Res., 5:
343 [ 0.1% Toluidine blue O in 2.5% carbonate at pH 11 (confirmed in
Lewis
and Knight below)]
2. Charles and Meek , G.R. separately in a discussion of
paper
by Mercer(1963), J Microscp Sci., 81:179. [mentions 1% Toluidine blue O
in
1% borax used at 85 degrees C]
3. Pease, D.C.(1964), Histological Techniques for Electron
Microscopy (2d), Academic Press, NY, NY, pp 256-262. [mentions on p. 260
in
2st paragraph the exact method that I use for staining as 'his' method -
"...flood the slide, cook until [edge] of drop [froths/]steams [or
dries]....". [This is a long discussion to which Pease devotes an
entire
section, but one can just FEEL his impatience with the unimportance to
HIM
of this issue. The book, after all, is NOT about light microscopy. The
semi-thin section is for orientation only, and hardly deserves any more
notice - especially if the light microscopists cannot SEE what is to be
seen
in the best material ever handed them.]
4. In Kay, D.H.(ed)(1965), Techniques for electron
Microscopy(2d), F.A. Davis, Philadelphia, ISBN: [NOT given - is this
book
pre-historic?!] The following is cited as THE reference for using
Toluidine
blue O: Benscome, Stone, Latta, and Madden (1959), J. biophys. biochem.
cytol, 5: 508.

I have just looked in Geoffrey Meek's book (1970), Practical
Electron Microscopy for Biologists, Wiley_Interscience, NY, NY, p.
456-457.
ISBN: 0-471-59030-4. 1% in 1% with no citation, though in this book he
has
purposely NOT used citations at all.

I have also consulted the Sorvall manual on "Thin Sectioning"(3d
Ed)(1973) as well as the SerVall manual "Thin Sectioning and Associated
Technics for Electron Microscopy"(1959) which apparently was distributed
with the MT-1. In the latter, only staining of Methacrylate sections
was
given and no mention of 1% *bor* of any kind. In the former, 1% borate
at
pH 4.5 is mentioned, but that is not relevant to this discussion.

I would bet that you might have some of these references. If
you do
not, let me know, and I will dig them up, in old Philadelphia, in Ben
Franklin's old stacks (at the U. of P(ennsylvania) as we call it). I
will
do it anyway, because I want to know, but I will do it quicker if you
tell
me I'm keeping you waiting.

Regards,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Sally Stowe
} Sent: Tuesday, July 3, 2001 3:37 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Tol blue/borax
}
}
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}
}
}
} Somebody just asked me the reference for toluidine blue/sodium borate
(1%
} each) which is our routine LM stain - I cant find an attribution in
any of
} about 10 books. Can anybody help? This person should not be
forgotten!
} One suggestion was Alsop?
}
} thanks
}
} Sally
}
}
}
} Dr Sally Stowe
} Facility Coordinator,
} ANU Electron Microscopy Unit
} Research School of Biological Sciences
} Australian National University
} Canberra ACT0200
} AUSTRALIA
} stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
} ph 02 6125 2743
} http://www.anu.edu.au/EMU
}
}
}
}
}



From daemon Mon Jul 9 14:10:14 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 9 Jul 2001 14:02:57 -0500
Subject: RE: Brightness/Contrast Calibration Standards

Contents Retrieved from Microscopy Listserver Archives
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Woody, I certainly agree with you,
but it seems Elaine was talking about qualitative or even
quantitative comparison of the specimens histograms (I hope
the specimens are flat). I think, the most straightforward thing
is to use one of the measured specimens with wide brightness and
contrast range. It makes no sense to calibrate BSE image for range
from Si to Pb just to look at specimens containing, say, Cu and Au.
The same for SE image. Polished specimen has much narrower signal
range for brightness and contrast than fractured specimen.
And, of course, for a microscope with a tungsten gun it is better to
work well in the saturation range to insure brightness stability.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: White, Woody N. [mailto:nwwhite-at-mcdermott.com]
} Sent: Monday, July 09, 2001 7:19 AM
} To: 'Schumacher, Elaine'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Brightness/Contrast Calibration Standards
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
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} ListServer-at-MSA.Microscopy.Com
} On-Line Help
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} --------------------------------------------------------------
} ---------.
}
}
} Elaine,
}
} A BSE standard can be made from almost any materials
} (suitable for SEM).
} The std should be ground and polished to remove topography
} contrast. What
} materials you choose will depend on the examination goals.
}
} This brings me to a point. I would not stress BSE "calibration" as a
} generally useful technique. Under VERY limited circumstances, such
} calibration/standardization can be beneficial. Routine use of this
} technique will guarantee that sooner or later an important
} phase will be
} missed when examining an unknown. When examining with BSE, I find it
} imperative to be "all over the map" with the BSE B&C to be
} sure I am not
} missing something. It is not unheard of to need two or more images to
} define what is happening. Sometimes there is simply not
} enough dynamic
} range presented in one image to perceive all variations in
} composition.
}
} Regards,
} Woody White, McDermott Technology, Inc.
}
} } -----Original Message-----
} } From: Schumacher, Elaine [mailto:efschuma-at-uop.com]
} } Sent: Friday, July 06, 2001 12:27 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Brightness/Contrast Calibration Standards
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } Fellow Microscopists,
} }
} } We're looking for suggestions for SEM standards to calibrate
} } brightness/contrast settings (for example, setting an auto
} } B/C feature in
} } the SEM software, so that the desired repeatable B/C level is
} } obtained by
} } multiple users). Our customers' goal is to do
} } correction/matching of gray
} } scale histograms between images of different samples, and
} } they would like to
} } make the process of adjusting B/C levels at the start of
} } image acquisition
} } to be as standardized as possible. The imaging might be done
} } in secondary
} } or backscattered mode, so suggestions for both would be appreciated.
} }
} } Thanks vey much for you input,
} }
} } Elaine
} }
} } Elaine F. Schumacher
} } Analytical Specialist
} } UOP LLC
} } 25 East Algonquin Road
} } Des Plaines, IL 60017-5017
} } Phone: 847-391-3403
} } Fax: 847-391-3719
} } Email: efschuma-at-uop.com
} }
} }
}
}


From daemon Mon Jul 9 14:16:24 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Mon, 09 Jul 2001 12:12:13 -0700
Subject: RE: GaN/Sapphire thinned by PIPS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sputter deposition of Cu on the back side of holed samples was a common problem
in one-sided thinning with the PIPS. The Cu came from the sample support posts
that the sample was waxed onto. As Richard Beanland suggested, a simple acid
cleaning (e.g., dilute nitric acid) removes the Cu effectively if the sample can
take this treatment. Alternatively, you could vapor deposit a thin coating of
rocksalt on the sample and later clean it off with water. Two-sided milling
will avoid gross sputter contamination, but--

As a general rule in ion milling, it's highly recommended to avoid using any
material in the sample fabrication or holder that you can't tolerate having in
your sample. Whether you use a Cu or Mo grid, or fabricate samples by
sandwiching the sample between Si blocks, for example, the nature of the ion
milling process just about guarantees some of this material will collect in the
sample cracks and crevices.

Larry Thomas
Pacific Northwest National Laboratory, Richland, WA, USA
larry.thomas-at-pnl.gov


---------
From: Ronald Anderson
Sent: Monday, July 9, 2001 6:45 AM
To: richard.beanland-at-marconi.com
Cc: Feng Wu; Microscopy Listserver
Subject: Re: GaN/Sapphire thinned by PIPS

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The main tricks are to low-angle mill both sides of the specimen and to
not
use Cu grids. We switched to Mo grids years ago and we rarely see back
sputtered material on samples milled on both sides (from the substrate
direction, of course). If you want to use up your stock of Cu grids,
cut
the back side of the grid away so that there is no grid material beyond
the
specimen to sputter from. Grid becomes the "C" shaped grid used for FIB
prepped specimens. Sometimes some sputtered material persists after
very
low angle milling. In that case, pop the sample into a conventional
high-angle mill and mill at 25 degrees for 30 seconds at 2 or 3 KeV to
clean off the sputter artifact.



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Richard Beanland +44 1327 356363 {richard.beanland-at-marconi.com} on
07/02/2001 07:02:41 AM

To: Feng Wu {fwu-at-bgumail.bgu.ac.il}
cc: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}
Subject: Re: GaN/Sapphire thinned by PIPS



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Feng,
the trick is not to have a hole in your specimen where the ions can
go
through and back-sputter the Cu on to your sample. So make your
specimen
big enough to cover up the hole in the support grid and stop ion milling
immediately when a hole appears. The problem will go away to some
extent
if you use the two-sided specimen holder. Alternatively, use an inert
grid
and dissolve the Cu with an acid after specimen prep. (Most acids are
unlikely to affect GaN or sapphire.)

Richard




}
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}
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}
}
} Dear All,
}
} I met some problems during I prepared the samples for GaN/sapphire
plan-view
} using tripod wedge technique. After polishing, the sample was thinned
by
pips
} in one side. However, The other side usually was covered by copper, as
a
result
} the sample became dark. It seems the unthinned side should be covered
something
} to prevent sputtered materials from growing on it. Do you have some
knowledge
} on it?
}
} Thanks in advance.
}
} Best regards.
}
} Feng
} **********************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} voice 972-8-6461473
} fax 972-8-6472944
} fwu-at-bgumail.bgu.ac.il
} **********************************************
}
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street
London
W1J 6AQ












From daemon Mon Jul 9 15:51:57 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Mon, 09 Jul 2001 16:40:31 -0400
Subject: adsorbtion problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the board,
This message.....

Hello,
My protein sample does not adsorb to the grid even after glow
discharg, it is a membrane protein complex with the membrane lipids
froming vescicles. Nothing show up after negative staining.
What should I do to improve the adsorption? I have tried quick dring
with filter paper after put the sample on the grid, but it does not
work.

Thank you
......was written by a student with good
intent and
fair grammar/spelling. I apologize, lesson learned. Thanks for the
help.

the real Mike Delannoy




From daemon Mon Jul 9 16:33:44 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 9 Jul 2001 17:28:45 -0400
Subject: Hazardous chemicals, etc.

Contents Retrieved from Microscopy Listserver Archives
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We as microscopists often receive requests for information about
specific chemicals or chemical derivatives which we are requested to
investigate or required to use in studies that are requested. The amount of
information available is staggering, but relevant information is not always
immediately available, and, for many of us, the means of access is not
always apparent.
The one datum that will provide you with the quickest access to
information is the Chemical Abstracts Registry Number(CAS Reg. or just CAS).
It is usually located in the catalog from which a chemical was ordered and
can be obtained from the supplier and used to search any chemical database
with specificity and speed.
What follows is what I believe to be a reasonable strategy for
free, rapid, and reasonable access to information about any chemical that
raises a question. I have limited the list to four, because I have found
what I need using no more. There are other sources, but my object was speed
and utility.

A. NIST Chemistry WebBook(ver. 2000): http://webbook.nist.gov/

B. ToxLine Hazardous Substance Database:
http://toxnet.nlm.nih.gov/cgi-bin/sis/htmlgen?HSDB

C. INTERNATIONAL CHEMICAL SAFETY CARDS (ICSCs) (NIOSH/WHO):
http://www.cdc.gov/niosh/ipcs/nicstart.html (US National Version)

D. Material Safety Data Sheets:
http://siri.org/msds/index.html (This is the only site that is NOT supported
by a government agency!)

For masters and students, I believe that the three URL's listed
above provide access to the best information about chemicals that are
considered hazardous.

DISCLAIMER: To assume that any chemical is harmless is foolish and
probably wrong. Many years ago there was a news report of a pre-adolescent
who woke up on 1November with diabetic acidosis after eating a grocery bag
full of candy collected the night before. Even NaCl or KCl can be toxic!!!
The Moral Is: If you get no return from any of the four above URL's for a
chemical name with which you are NOT familiar, check the name and require a
copy of the MSDS (Material Safety Data Sheet) BEFORE you permit it in your
lab.

To date, I have used Google for over a year to search for info on
everything including many organic chemicals. I have always received returns
on chemical searches.


Respectfully submitted,

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Mon Jul 9 21:03:15 2001



From: drcline-at-flash.net ()
Date: Mon, 9 Jul 2001 20:58:23 -0500
Subject: Ask-A-Microscopist:Looking for a Museum to Donate Slides

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(drcline-at-flash.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, July
9, 2001 at 17:07:43
---------------------------------------------------------------------------

Email: drcline-at-flash.net
Name: Diane Cline

Organization: George Washington University

Education: Graduate College

Location: Washington DC

Question: My grandmother, Mrs. Ethel Schwartz, is trying to find an
appropriate museum
or library to donate about 500 slides of my grandfather's, Dr. J.B.
Robbins, who
developed the technique of photography through a triocular microscope
of chemical crystals on slides in the late 196o's through early
1970's. Might you be able to suggest a collection (museum,
university archive, or Library) that might be especially interested?
Thank you, Sincerely
Diane Harris Cline

---------------------------------------------------------------------------


From daemon Mon Jul 9 22:26:03 2001



From: CachatF-at-aol.com
Date: Mon, 9 Jul 2001 23:18:34 EDT
Subject: stereology on thick sections

Contents Retrieved from Microscopy Listserver Archives
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I am interested in counting the total number of glomeruli in the kidneys in
} rats with obstructive uropathy. To perform stereology, I have to cut thick
} sections at 20 micrometers. I found that the kidney itself breaks down and
} get very damaged at this thickness (kidneys fixed in 10% formalin and
} embedded in parrafin), probably because the kidney is much harder than the
} paraffin. In the litterature, some people embed kidneys in glycol
} methacrylate. It might be better (harder), but then I do not know how to
cut
} my sections which are about 2.5 to 3 cm long, if I need a glass/diamond
knife
} that long. Any ideas about cutting thick sections in paraffin?, or is it
} worth re-embedding in glycolmethacrylate, and any ideas about cutting my
} serial sections at 20 micrometers in that resin?
}
} Thank you a lot for your help!
}
} F. Cachat
} University of Virginia
} fc6b-at-virginia.edu


From daemon Tue Jul 10 06:30:10 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 10 Jul 2001 21:22:09 +1000
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
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Bill - here is the animal toxicity data according to the MSDS sheets available
from our site.
Methylene Blue: Oral rat LD50: 1180mg/kg Mutation references cited. Animal
reproductive effects cited
Toluidine Blue O: IPR-Rat LD50: 215mg/kg; IVN-Rat LD50: 28930?g/kg; IVN-Mus
LD50: 45mg/kg; UNR-Mus LD50: 28mg/kg; IVN-Rbt LD50: 13440?g/kg.
In an ideal world that data would be comparable, but this is what we have and I
assume that its based on some realities.
I would not overly worry about the LD50 figures, unless they indicate great
toxicity. More concern is warranted if a toxin is cumulative and if its
carcinogenic or mutagenic. Mutagenicity is indicated for methylene blue and not
for toluidine blue O.
Since mutagenicity is one of the great fears during pregnancy, I would suggest
to revert to toluidine blue O.
I think that most people in this forum would understand that carcinogenic and
mutagenic molecules are ingested daily and in huge numbers. Clearly the body
wards off all of these and it is a very rare event that a cancer actually
forms. It is well known that a person with a healthy life-style and on a good
diet (particularly one rich in the anti-oxidant vitamins) is more likely to
avoid cancer. That healthy life-style includes minimizing, but never excluding
contact with "adverse" substances.
I suggest that one should be circumspect, but avoid paranoia. On balance, I
regard a well run EM lab as one of the safer workplaces about; it would be nice
to have comparative data of sick days or better still accident data, but I
doubt that they exist.
While on the topic of safety and carcinogens I like to tell a little story. It
happened at an unmentionable university about 15 years ago. The ceiling in one
large public room was found to contain fibres, which were confirmed to be
asbestos. After some agitation the university's response was to invite an
"expert", who was used by various organisations to "resolve" asbestos problems.
The expert soon had his first meeting with union officials during which he
insisted that asbestos was not very dangerous. To make his point, he opened a
jar allegedly containing powdered asbestos and threw a handful into the air.
Even 15 years ago, such action identified that man as an idiot. Educated
perhaps, but an idiot nonetheless.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, July 10, 2001 5:05 AM, William McManus
[SMTP:billemac-at-biology.usu.edu] wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} To Sally and all:
}
} I guess that my original post was too curt. We have gone away from Tol
} Blue, to methylene blue due to safety reasons. There is no toxicology
} data on the MSDS for Tol Blue, but there is for Meth Blue. As I have a
} pregnant technician, I feel better knowing the LD 50 information, which
} is on the MSDS for Meth Blue. But, methylene blue is not as good for a
} general stain as Tol Blue, so I was just curious if there was an
} overriding reason to use it. I am presently experimenting with food
} based stains, which eliminates the issue completely.
}
} The issue of pregnant (or possibly pregnant) workers in the lab has
} turned out to be fairly complicated. If anyone else out there has beem
} through this situation, I would like to know how you dealt with it.
}
} Bill
}
} William McManus
} Supervisor
} Electron Microscopy Facility
} Department of Biology
} Utah State University
} Logan UT 84322-5305
}
} billEMac-at-cc.usu.edu
} 435-797-1920
}
} -----Original Message-----
} } From: Monson, Frederick C. [mailto:fmonson-at-wcupa.edu]
} Sent: Thursday, July 05, 2001 10:20 AM
} To: 'Sally Stowe'
} Cc: 'Microscopy Listserver'
} Subject: RE: Tol blue/borax
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Dear Sally,
} To address the REAL attribution issue, one of us is going to
} have to
} go to a REALLY good library that has these OLD journals back to the
} late
} '50's. We may have to divide the labor, but the question is
} sufficiently
} interesting and meritorious that we ALL should get it right, when asked.
} Someone was responsible for the use of borax (Sodium tetraborate) with
} Toluidine blue O (1% in 1%). Here are all the citations that Pease
} provides
} and his [attributions]:
}
} 1. Trump, Schmuckler and Benditt (1961), J. Ultrastruc
} Res., 5:
} 343 [ 0.1% Toluidine blue O in 2.5% carbonate at pH 11 (confirmed in
} Lewis
} and Knight below)]
} 2. Charles and Meek , G.R. separately in a discussion of
} paper
} by Mercer(1963), J Microscp Sci., 81:179. [mentions 1% Toluidine blue O
} in
} 1% borax used at 85 degrees C]
} 3. Pease, D.C.(1964), Histological Techniques for Electron
} Microscopy (2d), Academic Press, NY, NY, pp 256-262. [mentions on p. 260
} in
} 2st paragraph the exact method that I use for staining as 'his' method -
} "...flood the slide, cook until [edge] of drop [froths/]steams [or
} dries]....". [This is a long discussion to which Pease devotes an
} entire
} section, but one can just FEEL his impatience with the unimportance to
} HIM
} of this issue. The book, after all, is NOT about light microscopy. The
} semi-thin section is for orientation only, and hardly deserves any more
} notice - especially if the light microscopists cannot SEE what is to be
} seen
} in the best material ever handed them.]
} 4. In Kay, D.H.(ed)(1965), Techniques for electron
} Microscopy(2d), F.A. Davis, Philadelphia, ISBN: [NOT given - is this
} book
} pre-historic?!] The following is cited as THE reference for using
} Toluidine
} blue O: Benscome, Stone, Latta, and Madden (1959), J. biophys. biochem.
} cytol, 5: 508.
}
} I have just looked in Geoffrey Meek's book (1970), Practical
} Electron Microscopy for Biologists, Wiley_Interscience, NY, NY, p.
} 456-457.
} ISBN: 0-471-59030-4. 1% in 1% with no citation, though in this book he
} has
} purposely NOT used citations at all.
}
} I have also consulted the Sorvall manual on "Thin Sectioning"(3d
} Ed)(1973) as well as the SerVall manual "Thin Sectioning and Associated
} Technics for Electron Microscopy"(1959) which apparently was distributed
} with the MT-1. In the latter, only staining of Methacrylate sections
} was
} given and no mention of 1% *bor* of any kind. In the former, 1% borate
} at
} pH 4.5 is mentioned, but that is not relevant to this discussion.
}
} I would bet that you might have some of these references. If
} you do
} not, let me know, and I will dig them up, in old Philadelphia, in Ben
} Franklin's old stacks (at the U. of P(ennsylvania) as we call it). I
} will
} do it anyway, because I want to know, but I will do it quicker if you
} tell
} me I'm keeping you waiting.
}
} Regards,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}
}
}
} } ----------
} } From: Sally Stowe
} } Sent: Tuesday, July 3, 2001 3:37 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Tol blue/borax
} }
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} }
} } Somebody just asked me the reference for toluidine blue/sodium borate
} (1%
} } each) which is our routine LM stain - I cant find an attribution in
} any of
} } about 10 books. Can anybody help? This person should not be
} forgotten!
} } One suggestion was Alsop?
} }
} } thanks
} }
} } Sally
} }
} }
} }
} } Dr Sally Stowe
} } Facility Coordinator,
} } ANU Electron Microscopy Unit
} } Research School of Biological Sciences
} } Australian National University
} } Canberra ACT0200
} } AUSTRALIA
} } stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
} } ph 02 6125 2743
} } http://www.anu.edu.au/EMU
} }
} }
} }
} }
} }



From daemon Tue Jul 10 08:03:30 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 10 Jul 2001 08:57:07 -0400
Subject: RE: Tol blue/borax-historic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jan,
Just to keep the historical stuff together on this thread, the
well-known Giemsa blood stain was first published, as a footnote, in "...an
obscure journal...." in (see Gray, P.(1954), The Microtomist's Formulary and
Guide, Blakiston, NY,NY. (reprinted by Krieger(1975), ISBN 0-88275-247-2).
The dry Giemsa stain is an eosinate of methylene blue [acid + base =
insoluble salt (in HOH but not in MeOH! Later, on the slide, soluble (mass
action) in large excess of MeOH-water or buffer mix yields differential
dyeing].

I searched the Krieger site (http://www.web4u.com/krieger-publishing/) for
ISBN and came up with the following:

Hosted by web4u.com!

Return to search form

Krieger Publishing Book Information

THE MICROTOMIST'S FORMULARY AND GUIDE
Peter Gray, ,
0-88275-247-2

Pages: 808, Binding: Cloth, Price: $81.50


Description:

This is a known and recognized source reference work. The book includes a
treatise on the art of making microscopic slides from biological specimens,
as well as a classified list of the formulas and techniques used in this
art.

Legal:

Orig. Ed 1954, Reprint Ed. 1975

Order copies of this book. Submit Order


Checkout

Return to search form

Krieger Publishing, Inc. Home
------------------------------------------------------------------------

Thanks for visiting with us!
Krieger Publishing
Melbourne, Florida

Use web4u.com's handy feedback form for any site issues.

NOTE: I am not connected except by long-familiarity (30+ years). Ouch!!!

Fred Monson


} ----------
} From: Jan Coetzee
} Reply To: janc-at-ccnet.up.ac.za
} Sent: Friday, July 6, 2001 3:55 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Re: Tol blue/borax
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all
}
} To contribute to the search for the roots of the Toluidine blue
} procedure:
}
} There is an interesting paper by Kramer & Windrum (J. Histochem
} Cytochem, 1955, Vol 3, 227-237, The Metachromic Staining Reaction)
} that probably was instrumental in initiating the wider use of Tol
} Blue. In this paper the effect of stain concentration and residual
} water is shown (amongst many other factors) to be important for the
} development of the multiplicity of colours.
}
} The use of Toluidine Blue as a stain for monitor sections of epoxy-
} and acrylic-embedded tissue probably stems from these metachromic
} effects that this stain produces, with the result that some deductions
} regarding the chemical composition of the sample can be made after a
} simple Tol Blue staining.
}
} Even though we, in this lab, have since forever used Tol Blue for the
} staining of monitor sections of resin-embedded tissues, every batch of
} Tol Blue that we make up seems to have slightly different staining
} characteristics. This was such a source of irritation that Chris van
} der Merwe recently decided to systematically investigate some of the
} variables.
}
} He found that the most important parameters are:
} 1. Tol blue concentration (0.1 or 0.5%)
} 2. Buffer or vehicle type, pH and concentration (0.1 - 1%
} Na-carbonate, Na-benzoate buffer at pH4, Na-tetraborate (borax) at pH
} 8.8)
} 3. Embedding resin formulation (Spurr, Quetol, Polarbed 812, LR-White)
} 4. Section thickness.
}
} All the above have an effect on the intensity, as well as the degree
} of metachromicity, of the stain.
} Resin formulation is probably the major influencer of the results.
}
} This may be a reason for the existence of the wide variety of Tol Blue
} recipes that abound in the literature.
}
} More information on Tol Blue staining can be had directly from Chris
} at cvdmerwe-at-scienti.up.ac.za
}
}
} Regards
}
} Jan Coetzee
} Lab for Microscopy and Microanalysis
} University of Pretoria, South Africa.
} Tel: 012-420-2075, Fax 012-362-5150
} www.up.ac.za/academic/electron/emunit1.htm
}
}
}
} "Monson, Frederick C." wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Dear Sally,
} } To address the REAL attribution issue, one of us is going to
} have to
} } go to a REALLY good library that has these OLD journals back to the
} late
} } '50's. We may have to divide the labor, but the question is
} sufficiently
} } interesting and meritorious that we ALL should get it right, when asked.
} } Someone was responsible for the use of borax (Sodium tetraborate) with
} } Toluidine blue O (1% in 1%). Here are all the citations that Pease
} provides
} } and his [attributions]:
} }
} } 1. Trump, Schmuckler and Benditt (1961), J. Ultrastruc
} Res., 5:
} } 343 [ 0.1% Toluidine blue O in 2.5% carbonate at pH 11 (confirmed in
} Lewis
} } and Knight below)]
} } 2. Charles and Meek , G.R. separately in a discussion of
} paper
} } by Mercer(1963), J Microscp Sci., 81:179. [mentions 1% Toluidine blue O
} in
} } 1% borax used at 85 degrees C]
} } 3. Pease, D.C.(1964), Histological Techniques for Electron
} } Microscopy (2d), Academic Press, NY, NY, pp 256-262. [mentions on p. 260
} in
} } 2st paragraph the exact method that I use for staining as 'his' method -
} } "...flood the slide, cook until [edge] of drop [froths/]steams [or
} } dries]....". [This is a long discussion to which Pease devotes an
} entire
} } section, but one can just FEEL his impatience with the unimportance to
} HIM
} } of this issue. The book, after all, is NOT about light microscopy. The
} } semi-thin section is for orientation only, and hardly deserves any more
} } notice - especially if the light microscopists cannot SEE what is to be
} seen
} } in the best material ever handed them.]
} } 4. In Kay, D.H.(ed)(1965), Techniques for electron
} } Microscopy(2d), F.A. Davis, Philadelphia, ISBN: [NOT given - is this
} book
} } pre-historic?!] The following is cited as THE reference for using
} Toluidine
} } blue O: Benscome, Stone, Latta, and Madden (1959), J. biophys. biochem.
} } cytol, 5: 508.
} }
} } I have just looked in Geoffrey Meek's book (1970), Practical
} } Electron Microscopy for Biologists, Wiley_Interscience, NY, NY, p.
} 456-457.
} } ISBN: 0-471-59030-4. 1% in 1% with no citation, though in this book he
} has
} } purposely NOT used citations at all.
} }
} } I have also consulted the Sorvall manual on "Thin Sectioning"(3d
} } Ed)(1973) as well as the SerVall manual "Thin Sectioning and Associated
} } Technics for Electron Microscopy"(1959) which apparently was distributed
} } with the MT-1. In the latter, only staining of Methacrylate sections
} was
} } given and no mention of 1% *bor* of any kind. In the former, 1% borate
} at
} } pH 4.5 is mentioned, but that is not relevant to this discussion.
} }
} } I would bet that you might have some of these references. If
} you do
} } not, let me know, and I will dig them up, in old Philadelphia, in Ben
} } Franklin's old stacks (at the U. of P(ennsylvania) as we call it). I
} will
} } do it anyway, because I want to know, but I will do it quicker if you
} tell
} } me I'm keeping you waiting.
} }
} } Regards,
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } West Chester University of Pennsylvania
} } Center for Advanced Scientific Imaging (CASI)
} } Schmucker II Science Center (Room: SS024(Basement))
} } South Church Street
} } West Chester, PA, 19383
} } MailDrop: Department of Geology/Astronomy
} } Phone: 610-738-0437
} } Fax: 610-436-3036
} } email: fmonson-at-wcupa.edu
} } Please call before visiting.
} }
} }
} } } ----------
} } } From: Sally Stowe
} } } Sent: Tuesday, July 3, 2001 3:37 AM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: Tol blue/borax
} } }
} } }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------.
} } }
} } }
} } }
} } } Somebody just asked me the reference for toluidine blue/sodium borate
} (1%
} } } each) which is our routine LM stain - I cant find an attribution in
} any of
} } } about 10 books. Can anybody help? This person should not be
} forgotten!
} } } One suggestion was Alsop?
} } }
} } } thanks
} } }
} } } Sally
} } }
} } }
} } }
} } } Dr Sally Stowe
} } } Facility Coordinator,
} } } ANU Electron Microscopy Unit
} } } Research School of Biological Sciences
} } } Australian National University
} } } Canberra ACT0200
} } } AUSTRALIA
} } } stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
} } } ph 02 6125 2743
} } } http://www.anu.edu.au/EMU
} } }
} } }
} } }
} } }
} } }
}
}


From daemon Tue Jul 10 08:07:23 2001



From: RRahbari-at-synapticcorp.com
Date: Tue, 10 Jul 2001 09:03:17 -0400
Subject: stereology on thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may have better luck using a vibratome. The kidney, being sharply
demarcated and well encapsulated, lends itself to processing in this manner.
I would fix before sectioning, (dependent on species donating the
kidney-probably not bigger than rat) but I have seen it work either way.
The fixative is dictated by stains/probes of interest. Using a cryotome and
a water soluble embedding media has also worked well for me in the past.
good luck

Ramin

-----Original Message-----
} From: "CachatF-at-aol.com"-at-sparc5.microscopy.com
[mailto:"CachatF-at-aol.com"-at-sparc5.microscopy.com]
Sent: Monday, July 09, 2001 11:19 PM
To: Microscopy-at-sparc5.microscopy.com


I am interested in counting the total number of glomeruli in the kidneys in
} rats with obstructive uropathy. To perform stereology, I have to cut thick

} sections at 20 micrometers. I found that the kidney itself breaks down and

} get very damaged at this thickness (kidneys fixed in 10% formalin and
} embedded in parrafin), probably because the kidney is much harder than the

} paraffin. In the litterature, some people embed kidneys in glycol
} methacrylate. It might be better (harder), but then I do not know how to
cut
} my sections which are about 2.5 to 3 cm long, if I need a glass/diamond
knife
} that long. Any ideas about cutting thick sections in paraffin?, or is it
} worth re-embedding in glycolmethacrylate, and any ideas about cutting my
} serial sections at 20 micrometers in that resin?
}
} Thank you a lot for your help!
}
} F. Cachat
} University of Virginia
} fc6b-at-virginia.edu


From daemon Tue Jul 10 08:37:34 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 10 Jul 2001 09:28:14 -0400
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 1:04 PM -0600 7/9/01, William McManus wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

When I was pregnant, 9 years ago, I was the entire staff of the EM
facility here (still was until a month ago)and so could not avoid
things. The way I handled safety concerns was to be extra cautious:
I worked in the hood, wore double gloves when working with fixatives
& resins and gloves for other times when i would not usually wear
them( like using Tol Blue), safety glasses if splashing was a
possibility, washed my hands almost compulsively. In short, I used
common sense. I was, and am, lucky that (UrAc aside) I don't have to
deal with radioactivity. colleagues of mine who were also pregnant
at the time (we had a rash of pregnancies that year) who did need to
work with isotopes minimized their use, got new shields, and at times
convinced labmates to do those steps for them!

We all, thankfully had healthy babies who are now all approaching
adolescence and driving us nuts, but that's another issue for another
forum!

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jul 10 09:52:21 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 10 Jul 2001 10:47:29 -0400
Subject: Re: stereology on thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"CachatF-at-aol.com"-at-sparc5.microscopy.com wrote:

} I am interested in counting the total number of glomeruli in the kidneys in
} } rats with obstructive uropathy. To perform stereology, I have to cut thick
} } sections at 20 micrometers. I found that the kidney itself breaks down and
} } get very damaged at this thickness (kidneys fixed in 10% formalin and
} } embedded in parrafin), probably because the kidney is much harder than the
} } paraffin. In the litterature, some people embed kidneys in glycol
} } methacrylate. It might be better (harder), but then I do not know how to
} cut
} } my sections which are about 2.5 to 3 cm long, if I need a glass/diamond
} knife
} } that long. Any ideas about cutting thick sections in paraffin?, or is it
} } worth re-embedding in glycolmethacrylate, and any ideas about cutting my
} } serial sections at 20 micrometers in that resin?
} }
} } Thank you a lot for your help!
} }
} } F. Cachat
} } University of Virginia
} } fc6b-at-virginia.edu

How about frozen sections? Fix in formalin, cryoprotect with ascending
concentrations of sucrose (10%, 20%, 30%) then snap freeze and section at 20
microns on a sliding microtome. Collect sections in ice-cube trays to keep them
in order.
In my experience, thick paraffin sections are much more difficult than thinner
ones. Embedding tissue in methacrylates (and other plastics) is usually intended
for thinner sections (1-3 microns).

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Jul 10 10:06:50 2001



From: Philip Mutch :      Philip.Mutch-at-nottingham.ac.uk
Date: Tue, 10 Jul 2001 15:35:24 +0100
Subject: Re: stereology on thick sections

Contents Retrieved from Microscopy Listserver Archives
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Dear F,

We read your message with great interest.i routinely fix kidney of approximately the same size but usually cut for stereological work -at-10u.
you do not state any info regarding processing times etc but if your tissue is brittle this could be due to numerous factors
over fixation
leaving in alcohol/clearing agent too long (especially chloroform as clearing agent)
( i come from a laboratory that hand processes, which gives more control over times processed compared to that from machines.)
i also would question the fixative i always use Bouins fixative with kidney and have never had any problems in sectioning.
i would suggest that you do not embed in glycolmethacrylate as this is for cutting thin's and also there is no way you would be able to get diamond knife to cut the size if tissue you want to. you could make ralph knives but again this will probably not work on the thickness you are after.

my last suggestion to you is regarding you 'already brittle blocks' try bakers soaking fluid (mixture of alcohol/glycerine) this should cure your problem but it is slow going as you will only get a few sections before they will need resoaking

i hope this works and is helpful to you
yours

Phil

Mr P R Mutch
Senior Imaging/Research Technician
Nottingham University
School of Biomedical Sciences
E Floor, Medical School
Queens Medical Centre
Clifton Boulevard
Nottingham
NG7 2UH
UK
Tel 0115 9709415
E-mail philip.mutch-at-nottingham.ac.uk

Mr P R Mutch
Senior Imaging/Research Technician
Nottingham University
School of Biomedical Sciences
E Floor, Medical School
Queens Medical Centre
Clifton Boulevard
Nottingham
NG7 2UH
UK
Tel 0115 9709415
E-mail philip.mutch-at-nottingham.ac.uk



From daemon Tue Jul 10 10:24:17 2001



From: Chen Chen :      cche1-at-mail.jhmi.edu
Date: Tue, 10 Jul 2001 11:19:37 -0400 (EDT)
Subject: please subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, my name is Chen Chen, in Johns Hopkins University School of Medicine.
Mike DeLannoy introduced me to subscribe to this group.
Thank you
Chen Chen



From daemon Tue Jul 10 10:33:28 2001



From: William McManus :      billemac-at-biology.usu.edu
Date: Tue, 10 Jul 2001 09:30:55 -0600
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
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to all out there:

This is what I have done so far:
1. Removed all stored chemicals from the lab.
2. Moved all chemical mixing into fume hood.
3. Met for 3 hours with environmental safety officer.
4. Met for 2 hours with radiation safety officer.
5. Eliminated all protocols which involve teratogens from pregnant
workers routine.
6. Established a personal hygiene plan for pregnant worker, which is
being signed of by worker, myself, director of the lab, safety
officer, and her physician.
7. Reiterated safety protocols for worker, coat , gloves, safety
glasses, etc.
8. Had lab monitored for radiation, passed.
9. Had lab monitored for formaldehyde gas, passed.
10. Planning to monitor xylene exposure to worker when thin sectioning
(cannot do this in the hood, obviously). Heat pen( modified soldering
iron) has not worked well so far, any ideas how to eliminate xylene?
11. Still looking for a non toxic general 1 micron stain for Spurrs and
LR White. Any ideas?
I am experimenting with food related stains (grape juice, etc.).
12. Employee has completed, lab safety training course, formaldehyde
training course and radiation training course.
13. Will continue to monitor lab for chemical and radation exposure.

To some this may seem like over kill, but I believe that it is very
important that every stone is turned in regards to safety. Another
issue that I face is that I have numerous grad students coming through
the lab, and there is always a possibility that they are pregnant and do
not realize it or keep it from me. I also have a nursing mother who was
visiting the lab and never bothered to tell me, until the subject of why
the lab was reorganized came up. I now demand that all female student,
techs, visitors, ect.
tell me of their "motherhood" status.

Bill

-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
Sent: Tuesday, July 10, 2001 7:28 AM
To: William McManus; Monson, Frederick C.; Sally Stowe
Cc: Microscopy Listserver

From daemon Tue Jul 10 11:14:45 2001



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Tue, 10 Jul 2001 12:06:37 -0400
Subject: Science Camp

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Dear Microscopists,

Every summer at our institute we sponsor a summer camp program called
Adventures in Science. We invite inner-city fifth graders to come and
hopefully see how much fun science can be. It has been rather
successful and we all seem to enjoy the week.

One of the scientists here wants to give a demonstration involving
vortices and fluids. He has a few short demonstrations to show the
kids, but I told him I think he needs more to keep their interest and
to keep them busy. He has five groups of 6 children for about an
hour.

I told him about the MSA newsgroup and how helpful you all are and
said I would post this question. I know this is not a microscopy
question but I thought some of you might have some suggestions.

Thanks in advance for any help.

Peggy Bisher
--


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com






From daemon Tue Jul 10 14:22:12 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 10 Jul 2001 12:05:03 -0700
Subject: RE: HMDS

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Thanks All ... for your cautions and info regarding HMDS. It gives good results, but I will definately use a hood or go back to my CPD!

Barbara Plowman
University of the Pacific
School of Dentistry
2155 Webster St.
San Francisco, CA 94115
email: BPlowman-at-sf.uop.edu




From daemon Tue Jul 10 14:50:02 2001



From: kszaruba1-at-mmm.com
Date: Tue, 10 Jul 2001 14:44:02 -0500
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
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Bill,

I commend you for your concern about safety. I went through two
pregnancies as a biological electron microscopist. Since I was the
solitary person doing TEM, I didn't have the choice of delegating
anything. I actually chose to delay an R&D project for my first trimester
because I didn't want to use uranyl acetate, osmium tetroxide/ruthenium
tetroxide and especially lead citrate and tannic acid stains all involved
in TEM. Other than that, I just used extra caution, especially when
weighing/handling powdered chemicals. I never paid much attention to the
Tol. blue - you have obviously looked into this in great detail.

I missed your original post. Are you trying to stain epoxy-embedded
sections for light microscopy? I've tried a number of food dyes in the
past and had very limited success. Also just because they are FD&C or D&C
approved doesn't guarantee safety. I've worked with Erioglaucine
(Brilliant Blue FCF, FD&C Blue #1) and the MSDS states it is a possible
carcinogen.

I've tried some non-traditional dyes on epoxy sections of mammalian
tissue. The standard Tol. Blue, "Epoxy Tissue Stain" (mixture with Basic
Fuchsin) and/or Azur II always worked the best. BTW, my MSDS's listed
Toluidine Blue as an irritant, but Methylene Blue as an irritant and a
mutagen. Therefore I would think Tol. Blue was a little safer.

Excerpts from an experiment I did in '97 trying different stains on
~1micron sections of skin embedded in Spurr's resin (also not a good
safety choice):
Dyes tested:
0.1% aqueous Phloxine B. [related to eosin, a useful D&C approved dye for fresh tissue and
fluorescence. ]
0.15% Sudan Black B in 70% ethanol.
0.5% aqueous Neutral Red. [a fairly nontoxic dye (although MSDS states possible mutagen) that we use
a lot in fresh tissue.]
Zymed Methyl Green.
All were done on a hotplate for 30-60 seconds

Staining Results:
Phloxine B seemed to stain only the dermal collagen with a fairly bright
pink stain.
Sudan Black B produced an interesting pattern of staining small elongated
strips throughout the dermis, as well as intracellular staining of basal
epidermal cells. The fat cell staining was non-uniform.
Neutral Red stained in a very similar pattern to Sudan Black B in the
dermis, only much more faint.
Methyl Green produced a strong general stain similar but not exactly the
same as Toluidine Blue.


Hope this is useful,
Karen
--------------------------------------------------------------------------------
Karen S. Zaruba
BioMaterials Technology Center
3M Center Bldg. 270-1S-01
Tel: (651) 737-2971
Fax: (651) 737-5645




"William McManus" {billemac-at-biology.usu.edu}
07/09/01 02:04 PM


To: "Monson, Frederick C." {fmonson-at-wcupa.edu}
"Sally Stowe" {STOWE-at-rsbs.anu.edu.au}
cc: "Microscopy Listserver" {microscopy-at-sparc5.microscopy.com}
(bcc: Karen S. Zaruba/US-Corporate/3M/US)
Subject: RE: Tol blue/borax



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To Sally and all:

I guess that my original post was too curt. We have gone away from Tol
Blue, to methylene blue due to safety reasons. There is no toxicology
data on the MSDS for Tol Blue, but there is for Meth Blue. As I have a
pregnant technician, I feel better knowing the LD 50 information, which
is on the MSDS for Meth Blue. But, methylene blue is not as good for a
general stain as Tol Blue, so I was just curious if there was an
overriding reason to use it. I am presently experimenting with food
based stains, which eliminates the issue completely.

The issue of pregnant (or possibly pregnant) workers in the lab has
turned out to be fairly complicated. If anyone else out there has beem
through this situation, I would like to know how you dealt with it.

Bill

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920







From daemon Tue Jul 10 14:59:48 2001



From: William McManus :      billemac-at-biology.usu.edu
Date: 7/10/01 9:30 AM
Subject: RE: Tol blue/borax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck:

What I do is after they are hired, or at the beginning of the class, in
front of witnesses, I explain that for their own safety that I must know
if they are pregnant, so that all the appropriate precautions are taken.
I also tell them that this information will be kept in strict
confidence. It is pretty easy for people to figure out what is going
on, however, once you start treating one person differently than the
rest.

Bill

-----Original Message-----
} From: Chuck Butterick [mailto:cbutte-at-ameripol.com]
Sent: Tuesday, July 10, 2001 1:31 PM
To: William McManus


------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America

On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

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to all out there:

This is what I have done so far:
1. Removed all stored chemicals from the lab.
2. Moved all chemical mixing into fume hood.
3. Met for 3 hours with environmental safety officer.
4. Met for 2 hours with radiation safety officer.
5. Eliminated all protocols which involve teratogens from pregnant
workers routine.
6. Established a personal hygiene plan for pregnant worker, which is
being signed of by worker, myself, director of the lab, safety

officer, and her physician.
7. Reiterated safety protocols for worker, coat , gloves, safety
glasses, etc.
8. Had lab monitored for radiation, passed.
9. Had lab monitored for formaldehyde gas, passed.
10. Planning to monitor xylene exposure to worker when thin sectioning
(cannot do this in the hood, obviously). Heat pen( modified
soldering
iron) has not worked well so far, any ideas how to eliminate
xylene?
11. Still looking for a non toxic general 1 micron stain for Spurrs and
LR White. Any ideas?
I am experimenting with food related stains (grape juice, etc.).
12. Employee has completed, lab safety training course, formaldehyde
training course and radiation training course.
13. Will continue to monitor lab for chemical and radation exposure.

To some this may seem like over kill, but I believe that it is very
important that every stone is turned in regards to safety. Another
issue
that I face is that I have numerous grad students coming through the
lab,
and there is always a possibility that they are pregnant and do not
realize it or keep it from me. I also have a nursing mother who was
visiting the lab and never bothered to tell me, until the subject of why

the lab was reorganized came up. I now demand that all female student,
techs, visitors, ect.
tell me of their "motherhood" status.

Bill

-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
Sent: Tuesday, July 10, 2001 7:28 AM
To: William McManus; Monson, Frederick C.; Sally Stowe
Cc: Microscopy Listserver


At 1:04 PM -0600 7/9/01, William McManus wrote:
} -----------------------------------------------------------------------

-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Tue Jul 10 16:54:23 2001



From: Louis Somlai :      lssomlai-at-www.psrc.usm.edu
Date: Tue, 10 Jul 2001 17:07:40 -0500 (CDT)
Subject: images of blood

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Hi,

I'm looking for anyone who may have images of red blood cells, white blood
cells, and platelets. I'm also looking for an illustration of haemoglobin
binding. My mother-in-law is working on an educational web page about
blood for grades 4-6. All images will be referenced and properly credited.
If you can help, I would greatly appreciate it.

Thanks,

Louis
Louis.Somlai-at-usm.edu



From daemon Tue Jul 10 17:20:55 2001



From: Amy McGough :      amcgough-at-bilbo.bio.purdue.edu
Date: Tue, 10 Jul 2001 17:17:42 -0500
Subject: Postdoctoral position: Dynamics of biological machines

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Postdoctoral position(s): Dynamics of biological machines

Postdoctoral position(s) are available in the McGough lab at Purdue
University to study biological machines by a variety of microscopic,
computational, and biochemical approaches. Current projects in the lab
include structure-function studies of the cytoskeleton and bacteriophage
assembly.

Successful candidate(s) will work in close collaboration other
laboratories at Purdue in cross-disciplinary studies using a variety of
approaches including protein biochemistry, video light microscopy,
time-resolved electron cryomicroscopy, atomic force microscopy, magnetic
tweezers, and molecular modeling. Highly qualified candidates will be
encouraged to develop new collaborations involving members of the
cytoskeleton, structural biology, and single molecule groups at Purdue.

Applicants should have a Ph.D. in engineering or in one of the physical
or life sciences. The ideal candidate will be a hard-working,
enthusiastic individual with strong communication skills, experience in
protein biophysics, and a commitment to applying an integrated
structure-function approach to study biological machines.

Salary is negotiable.

Interested individuals should contact:

Dr. Amy McGough
Department of Biological Sciences
Purdue University
West Lafayette, IN 47907-1392 USA
phone: +1 (765) 496-7501
fax: +1 (765) 496-7501
amcgough-at-bilbo.bio.purdue.edu
http://bilbo.bio.purdue.edu/~amcgough/

Purdue is an equal access/equal opportunity university.




From daemon Tue Jul 10 17:38:20 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 10 Jul 2001 15:32:52 -0700
Subject: Re: SEM Service Life?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 03:21 PM 7/2/2001, you wrote:

[snip]

} On the other hand, if newer technologies could improve your performance
} significantly, then you might want to consider upgrading. The
} downside? When the PC that is running the latest system becomes obsolete
} (3 years? 5 years?) and the rest of your system (vacuum, High Voltage,
} etc.) is still fine, what are you going to do? Try to find a new 486 with
} Win 3.1 to replace a crashed but fairly recent Amray, for example. Their
} boards and software won't run on anything newer. I'm not picking on them,
} I'm just familiar with them.

I'm running an Amray 1910FE with PC control over the Nibblenet.
The system came with a Dell 486 and 250MB hard drive. I am
actually using a Pentium III/450 with Win95 or Win98 (either
works fine). The 8741 MPU on the Nibblenet board in the PC
needs the latest firmware version (2.3 or 2.4) to run with a
Pentium. The board is hardwired to IRQ9. With a simple
PC system, there are no IRQ conflicts.

I do not use the Image Control software/hardware (Matrox IP8)
but rather use Soft Imaging ADDA II for active beam control
and image capture and the GrabBit frame grabber to capture
TV video. Console electronics are un-changed. PC-10 video
goes to one of the GrabBit RS-190 inputs. The other input is
from the chamber view camera.

So far, KLA/Amray is still supporting the FE systems.

gary g.



From daemon Tue Jul 10 22:42:59 2001



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Tue, 10 Jul 2001 23:44:40 -0400
Subject: Re: Science Camp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peggy Bisher wrote:

} One of the scientists here wants to give a demonstration involving
} vortices and fluids. He has a few short demonstrations to show the
} kids, but I told him I think he needs more to keep their interest and
} to keep them busy. He has five groups of 6 children for about an
} hour.

Hi, Peggy --

I'll give you a great idea that takes about ten bucks worth of parts
and a source of compressed air. How about a gizmo made of two
1/2-inch (OK, Ok, 12mm) pipes, a washer, and a ball valve which
appears to contain Maxwell's demon. Blow compressed air in
the middle and presto, hot air comes out one pipe and cold air
comes out the other! It's called a Hilsch tube, and here's a link:

http://www.visi.com/~darus/hilsch/

If you do a search on "hilsch tube" you can find some commercial
suppliers of ready-made units.

I remember seeing it in an old issue of Scientific American, the
Amateur Scientist column. An outfit called Tinker's Guild, which
I believe to be the creation of Shawn Carlson, current author of
the Amateur Scientist, is offering a CD containing *every Amateur
Scientist column written since 1928* for a snappy $70. Quite
the bargain:

http://www.tinkersguild.com

Any of you who are science educators should get a copy of
this! No commercial interest, just a fascinated customer who
remembers actually trying quite a few of these as a kid.

Rick Mott




From daemon Wed Jul 11 03:02:56 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Wed, 11 Jul 2001 09:53:49 +0200
Subject: biofilm inclusion - any ideas?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

We have a student who has been looking at biofilms on artificial
membranes and in the process found a strange-looking inclusion. If
there's anyone out there who has the time to have a look at this (it
is displayed on http://www.ru.ac.za/emu/lechbug.htm) and give any
suggestions this will be much appreciated.

Regards

Rob


=======================================================
Robin H Cross (Mr)
Director : Electron Microscopy Unit
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
email: r.cross-at-ru.ac.za
http://www.ru.ac.za/emu/index.htm

** remember ICEM-15 in Durban in September 2002 **


From daemon Wed Jul 11 04:50:06 2001



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 11 Jul 2001 04:41:14 -0500
Subject: Re: Science Camp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Rick Mott" {rickmott-at-alumni.princeton.edu}
}
} Peggy Bisher wrote:
}
} } One of the scientists here wants to give a demonstration involving
} } vortices and fluids. He has a few short demonstrations to show the
} } kids, but I told him I think he needs more to keep their interest and
} } to keep them busy. He has five groups of 6 children for about an
} } hour.
}
} Hi, Peggy --
}
} I'll give you a great idea that takes about ten bucks worth of parts
} and a source of compressed air. How about a gizmo made of two
} 1/2-inch (OK, Ok, 12mm) pipes, a washer, and a ball valve which
} appears to contain Maxwell's demon. Blow compressed air in
} the middle and presto, hot air comes out one pipe and cold air
} comes out the other! It's called a Hilsch tube, and here's a link:
}
} http://www.visi.com/~darus/hilsch/
}
} If you do a search on "hilsch tube" you can find some commercial
} suppliers of ready-made units.
}
} I remember seeing it in an old issue of Scientific American, the
} Amateur Scientist column. An outfit called Tinker's Guild, which
} I believe to be the creation of Shawn Carlson, current author of
} the Amateur Scientist, is offering a CD containing *every Amateur
} Scientist column written since 1928* for a snappy $70. Quite
} the bargain:
}
} http://www.tinkersguild.com
}
} Any of you who are science educators should get a copy of
} this! No commercial interest, just a fascinated customer who
} remembers actually trying quite a few of these as a kid.
}
The Amateur Scientist is no more. It got cut along with most of the other
columns. It looks like a very large short fall in advertising revenues are a
problem. The adds are almost all consumer advertising. Not much that match
the readership. With some of the politically correct stuff they have been
publishing the last few years I expect that the scientific readership is
being run off and it is over the lay readerships head. I was particularly
put off by their piece on terrorism in the USA with out a any mention of
environmental terrorism.

The Amateur Scientist got a great number of us started on the road to
science it is a shame that it is being let go.

I have heard Fry's has the CD-ROM on sale for $25 with a $25 mail in rebate.
Now that's a deal.

Gordon

Gordon Couger
Stillwater, OK





From daemon Wed Jul 11 07:34:41 2001



From: David Spector at Cold Spring Harbor Laboratory :      spector-at-cshl.org
Date: Wed, 11 Jul 2001 08:14:33 -0400
Subject: Position Available for Biological Microscopy Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Core Technician

Cold Spring Harbor Laboratory is seeking an experienced and
responsible microscopy
technician for the laboratory's core biological microscopy facility.
The individual
should have practical expertise in transmission electron microscopy,
confocal and
widefield fluorescence microscopy, and digital imaging. The successful
candidate will be involved in designing and carrying out experimental
protocols for users,
training individuals in the use of various microscopes, and aligning
microscopes and keeping
the facility operating at an efficient and high level of
productivity. Interested
individuals should send their resume, including a description of
their expertise and
the names and addresses of 3 references to: Dr. David L. Spector,
Cold Spring Harbor
Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724,
email: spector-at-cshl.org
--
David L. Spector
Cold Spring Harbor Laboratory
One Bungtown Road
Cold Spring Harbor, New York 11724
Tel. (516) 367-8456
Fax (516) 367-8876
email: spector-at-cshl.org


From daemon Wed Jul 11 08:05:26 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 11 Jul 2001 09:02:27 -0400
Subject: Video wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone help this guy??



} Dear Sir,
}
} I am a media artist based in Canada. I am presently working on a low
} budget, art funded (Canada Council for the Arts) DVD project destined for
} non-profit art gallery use.
}
} The work is a philosophic and poetic look at evolution and bio-diversity.
}
} I am very interested in getting and using some of the video your members may
} have made on primitive life forms such as viruses, bacteria, etc.
} I would be also interested in
} acquiring from you a high quality beta-sp or preferable dv tape you might
} have of these studies as well as other studies you may have of microscopic
} life forms/elements dna, diatoms, etc. Full credit to your organization
} (and the videographer if desired) would be given in the final piece. I
} would pay for all shipping and handling costs.
}
}
}
} The video and images would be a small component of a much longer work.
}
} My last work recently screened at the Canadian Embassy in Washington, D.C.
} and I have had shows at the Museum of Modern Art in New York City and at
} many other prestigious venues around the world. I include my bio. below.
}
} Please get back to me regarding my request.
}
}
} Best Wishes,
} Oliver Hockenhull
}
} BIO - 2001 Oliver Hockenhull
}
} Home Site: http://www.crosswinds.net/~ideoplastic/
} (From this site link to various works in VRML, hypertext, etc.)
}
} Oliver Hockenhull is an award-winning media artist, director, communication
} theorist, and lecturer.
}
} He has completed five feature length works and numerous dramatic and
} experimental shorts. The films have shown internationally at such film
} festivals and venues as -(selected) The Kerala-India International Film
} Festival, The Montréal World Film Festival, The Leipzig International
} Documentary Film Festival, The Vancouver International Film Festival, The
} European Media Arts Festival, The Nouveau Cinema Festival , The
} International Documentary Film Festival of Amsterdam, The Museum of Modern
} Art in New York City, the National Gallery of Art in Washington, D.C., and
} LACE (Los Angeles Contemporary Exhibitions).

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Ditrector, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Wed Jul 11 08:25:08 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 11 Jul 2001 09:17:53 -0400
Subject: Science Camp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A neat topic is eddy currents. There are a number of ways to experiment with them.

My son did a project that I helped him with. I first showed him the effect by getting a magnet and tying a string to it and used it as a pendulum. We counted the number of periods for it to stop. Then we got a an aluminum tray and put it under the swinging magnet and counted again. We changed the distance of the tray to see the effect.

Then we got fancy. We built a swinging pendulum in which we could put sheets of metals that we got from the hobby store. We used aluminum, brass, and titanium. The sheets could pass through magnets that we set up in epoxy on a board that fit onto the base of the pendulum with pegs so that they put in the same place every time. We made two sets of magnets with different gaps. The magnets were bought at Radio Shack. We experimented with different thickness of sheets, and stacks of sheets to make the same thickness. We experimented with sheets of paper that isolated the sheets of metal. It was really a nice little project that illustrated a number of effects.

To get even more fancy, you could do the same things with disks of metal rotating on an inexpensive dc motor. You could then monitor the current with a meter to measure the power change with different magnetic fields.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Peggy Bisher [mailto:peggy-at-research.nj.nec.com]
Sent: Tuesday, July 10, 2001 12:07 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Microscopists,

Every summer at our institute we sponsor a summer camp program called
Adventures in Science. We invite inner-city fifth graders to come and
hopefully see how much fun science can be. It has been rather
successful and we all seem to enjoy the week.

One of the scientists here wants to give a demonstration involving
vortices and fluids. He has a few short demonstrations to show the
kids, but I told him I think he needs more to keep their interest and
to keep them busy. He has five groups of 6 children for about an
hour.

I told him about the MSA newsgroup and how helpful you all are and
said I would post this question. I know this is not a microscopy
question but I thought some of you might have some suggestions.

Thanks in advance for any help.

Peggy Bisher
--


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com






From daemon Wed Jul 11 08:28:10 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 11 Jul 2001 09:26:04 -0400
Subject: Fwd: Video wanted,part 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Sorry, forgot to include his email.
}
}
}
} Can anyone help this guy??
}
}
}
} } Dear Sir,
} }
} } I am a media artist based in Canada. I am presently working on a low
} } budget, art funded (Canada Council for the Arts) DVD project destined for
} } non-profit art gallery use.
} }
} } The work is a philosophic and poetic look at evolution and bio-diversity.
} }
} } I am very interested in getting and using some of the video your members may
} } have made on primitive life forms such as viruses, bacteria, etc.
} } I would be also interested in
} } acquiring from you a high quality beta-sp or preferable dv tape you might
} } have of these studies as well as other studies you may have of microscopic
} } life forms/elements dna, diatoms, etc. Full credit to your organization
} } (and the videographer if desired) would be given in the final piece. I
} } would pay for all shipping and handling costs.
} }
} }
} }
} } The video and images would be a small component of a much longer work.
} }
} } My last work recently screened at the Canadian Embassy in Washington, D.C.
} } and I have had shows at the Museum of Modern Art in New York City and at
} } many other prestigious venues around the world. I include my bio. below.
} }
} } Please get back to me regarding my request.
} }
} }
} } Best Wishes,
} } Oliver Hockenhull
hereticfilms-at-home.com

} } BIO - 2001 Oliver Hockenhull
} }
} } Home Site: http://www.crosswinds.net/~ideoplastic/
} } (From this site link to various works in VRML, hypertext, etc.)
} }
} } Oliver Hockenhull is an award-winning media artist, director, communication
} } theorist, and lecturer.
} }
} } He has completed five feature length works and numerous dramatic and
} } experimental shorts. The films have shown internationally at such film
} } festivals and venues as -(selected) The Kerala-India International Film
} } Festival, The Montréal World Film Festival, The Leipzig International
} } Documentary Film Festival, The Vancouver International Film Festival, The
} } European Media Arts Festival, The Nouveau Cinema Festival , The
} } International Documentary Film Festival of Amsterdam, The Museum of Modern
} } Art in New York City, the National Gallery of Art in Washington, D.C., and
} } LACE (Los Angeles Contemporary Exhibitions).
}
} Gregory W. Erdos, Ph.D.
} Assistant Director, Biotechnology Program
} Scientific Ditrector, ICBR EM CORE
} University of Florida Ph. 352-392-1295
} PO Box 118525 Fax 352-846-0251
} Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl
}

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Ditrector, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Wed Jul 11 08:54:54 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 11 Jul 2001 14:48:32 +0100 (GMT Daylight Time)
Subject: Re: biofilm inclusion - any ideas?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think it is a testate amoeba. They have a shell made of
imbricated silacous scales. I saw them once on a filter
from a water company.

Dave


On Wed, 11 Jul 2001 09:53:49 +0200 "R. Cross"
{r.cross-at-ru.ac.za} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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}
}
} Dear Colleagues
}
} We have a student who has been looking at biofilms on artificial
} membranes and in the process found a strange-looking inclusion. If
} there's anyone out there who has the time to have a look at this (it
} is displayed on http://www.ru.ac.za/emu/lechbug.htm) and give any
} suggestions this will be much appreciated.
}
} Regards
}
} Rob
}
}
} =======================================================
} Robin H Cross (Mr)
} Director : Electron Microscopy Unit
} Rhodes University, PO Box 94, Grahamstown, South Africa
} tel: +27 46 603 8168/9, fax: +27 46 622 4377
} email: r.cross-at-ru.ac.za
} http://www.ru.ac.za/emu/index.htm
}
} ** remember ICEM-15 in Durban in September 2002 **
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Jul 11 09:10:15 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Wed, 11 Jul 2001 10:05:21 -0400
Subject: RE: more on that scam

Contents Retrieved from Microscopy Listserver Archives
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} Hello listers,
} I recall seeing that thread late last week about the business opportunity.
} This just came in from a Canadian news source, CBC.
} Nick
} RCMP make arrests in international phone scam
} WebPosted Tue Jul 10 17:27:29 2001
} TORONTO - The RCMP, along with the FBI and the U.S. Secret Service, have
} cracked a multi-million dollar scam originating in Nigeria and arrested
} three people in the Toronto area.
} More than 300 people around the world - most of them Americans - were
} duped into giving money to a fake organization. The RCMP say some people
} lost more than $50,000. Others lost millions.
} Police say the fraud was sophisticated, high tech and organized.
} Typically, victims received a letter either by mail or fax allegedly from
} officials of a large Nigerian institution, such as the Nigerian National
} Petroleum Corporation or the Central Bank of Nigeria.
} The letters would contain an urgent request for help in transferring
} millions of dollars out of Nigeria to financial institutions in the
} victim's country. If the victims would help, they'd be in line for a
} handsome profit, in some cases as much as 30 per cent of the monies being
} transferred.
} The letter says foreign accounts are needed and victims were asked to wire
} personal banking information and $10,000 US to cover administration fees.
} 'Boiler room' in Toronto
} Then, victims were contacted by a person posing as a merchant banker of
} the Central Bank of Nigeria. They were told the funds were now in a
} clearing house or mercantile bank in North America.
} Phone numbers given for the fake banks actually rang in a "boiler room" in
} the Toronto area.
} The scam continued with the victim asked to pay taxes, duties and other
} fees, all with the promise of a windfall, until the victim was bankrupt.
} The RCMP began a co-ordinated effort with U.S. police forces in 1998.
} According to an RCMP news release, police detachments in the Toronto area
} have received at least one complaint a week for the past eight years from
} victims who lost money in the scam.
} Staff Sergeant Darryl Ross says victims lost more than money. Some
} suffered health problems, mental breakdowns, divorce and the break-up of
} their families.
}
}


From daemon Wed Jul 11 10:14:49 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Wed, 11 Jul 2001 11:06:41 -0400 (EDT)
Subject: magnetic field shielding

Contents Retrieved from Microscopy Listserver Archives
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Hi colleagues,

Can anyone recommend a good/cost-effective type of wall shielding to
eliminate 60 and 180 cycle interferences coming into an SEM room from
recently-installed large motors on the opposite side of the wall (that
can't be moved now)?

Much obliged,
Dee




***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Wed Jul 11 10:30:44 2001



From: swiding :      swiding-at-astro.temple.edu
Date: Wed, 11 Jul 2001 11:25:51 -0400
Subject: TEM literature

Contents Retrieved from Microscopy Listserver Archives
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Hello List,

Does anyone have any documentation/product specifications for a Philips
EM400 and EM410? We need the information for a possible upgrade.

Thanks,

Steve Widing
Biology Department
Temple University



From daemon Wed Jul 11 10:34:17 2001



From: Caroline Miller :      camiller-at-creighton.edu
Date: Wed, 11 Jul 2001 10:30:30 -0500
Subject: MSA Meeting

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As chairperson of the Traveling Poster Exhibit I am looking for judges
for the Poster Award Program. Anyone who will be attending the MSA
meeting at Long Beach and would be interested in helping please contact
me. I need five judges for each discipline, Biological and Material
Sciences. Thank you, Caroline Miller



From daemon Wed Jul 11 11:17:40 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 11 Jul 2001 12:10:15 -0400
Subject: RE: stereology on thick sections

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Dear Goeff,
Paraffin embedding works best on tissues that have some internal
support, such as a collagen matrix (e.g., skin, etc.). If you think about
the matter, the treatment to which tissues are subjected is harsh. Kidney
tends to dehydrate, clear and infiltrate more slowly. Further, any
imperfection in those steps can lead to a hard, fractious tissue.
I understand what you want to do, and, while I should not
overextend, I feel if I'm going to help with one, I may take the liberty of
helping with all.

First. Whole kidney is very hard to infiltrate with paraffin
without either segmenting it or removing the capsule (which is not always
easy). I always, at least, opened the capsule. This is probably necessary,
because as dehydration, clearing and infiltration proceed the capsule
shrinks with the organ. If the capsule shrinks more, which is my conclusion
on the matter, the internal pressure might be sufficient to retard the
processes.
Second. If 'First' is true, then the heat will essentially dry the
parenchyma of the organ and leave you with the impression that it is 'hard'.
It is interesting, that such blocks, when faced into the tissue always
respond positively to immersion to glycerinated water (10-20%). Of course,
all this manipulation does not leave one in the mood to try to finish with
something quantitative, so some means to avoid the problem must be devised.

Solutions. 1. I agree with the frozen section idea, if you
can take serial sections, then you can dye/stain and count every section if
you wish or some statistical sample designed for glomeruli.
2. I would try a milder dehydration routine and
vacuum embedding/infiltrating, at the lowest temperature, for 48 hours. My
experience has been with whole organs, and these can cause problems. A
milder dehydration does not take more than 1.5 the time but softens the
steps (e.g., instead of 30,70,95,100 EtOH, benzene you could use 20% steps
in EtOH followed by 1:2,1:1,2:1 (Benzene:EtOH) and 1:1(Benzene:Paraffin)
sub-steps (no longer than 1.25hr) and all but the benzene:paraffin steps at
4oC.

NOTE: Tt has been said that the most arduous transition is to 70%EtOH,
thus, if you accept that, then one ought to be able to leave tissue for long
periods at higher concentrations. I once experimented with the final
dehydration step in 100EtOH (and I mean 100%(200 Proof)) by placing a piece
of tissue in absolute EtOH in my -10oC freezer with a pint bottle of 200
Proof. I changed the fluid each day for a week and then finished the
processing. I saw no difference between the tissue processed at RT and that
dehydrated at -10oC, but if I had to try a whole kidney, I would remember
that brief experiment in the execution of the art of tissue processing if I
had infiltration problems.

Solution (My own approach).
I would take control kidneys, since rat kidney is unipolar
as I recall, and take transverse segments of known thickness (single-edge
razor blade) through the fixed kidney (laying on its side or held 'up' with
the 'ends of the bean' down) being careful to isolate the segments in order.
I would then process each segment between leaves of fine mesh, using vacuum
infiltration for the shortest time and at the lowest temperature. Following
installation of each segment in a block, I would carefully orient each block
to permit me the largest return of serial sections {10um. The ribbons of
serial sections would be placed on poster board in order. Knowing the mean
diameter of glomeruli, I would choose a sub-serial sample of sections from
each segment and mount them on slides, in order. Meanwhile, my notebook
would be filling with the numbers of the sections from the series of each
segment (A4-20-1)=Slide Label = Segment A, Start w/section 4, every 20th
section, slide 1), etc. Each slide with a mounted series would be marked
with a diamond scribe as 1,2,... and then as above - record in notebook. I
would then stain each slide with the PAS (periodic acid Schiff) reaction for
carbohydrates (BUT in kidney, really does a great job on glomeruli, and one
can use a green filter to count and photograph). A nuclear counterstain is
optional depending on your purpose, BUT then, there are hundreds of other,
adjacent sections waiting for such elaboration if you need them. If the
PA(to generate aldehydes from vicinyl glycols) and the S(especially!) are
fresh and tested (20ml of S with a drop of formaldehyde to prove the color
change from none to intense magenta), the PAS is almost entirely
predictable and effortless(?).
I would then count the glomeruli in each section or in a
statistically predetermined sub-sample, multiply, and get ready for the
pathologic specimens.
NOTE: Tissue dryness can occur even with the best laid
plans and execution, so I have one more suggestion - which will, of course,
eliminate the PAS as an appropriate stain. Consider using Polyethylene
Glycol(PEG) as an embedment. For those who have learned the method, it is
easy and productive, though I know of no reconstructive or statistical
methods that have been based on sections of PEG-infiltrated material.
NOTE: In any morphometric analysis, it is the sampling
method that makes the entire process meaningful or not. Take for example
the alternate experiment on a sheep. Would you have the same plan as you
proposed to us? What if you wanted to compare mouse and elephant with
respect to the structure of a single cell type. What are the statistical
implications of the problem? [RHETORICAL QUESTION!!!!!!!!] What you might
really like is to be able to take 10 random sections from each serially
sectioned segment of a kidney and demonstrate not only the differences
between normal and pathologic organs but also the presence or absence of any
variation in the distribution of pathology in any organ. Would you expect
the same result in a mouse or rabbit which also have unipolar kidneys? What
about your expectation in a multipolar kidney like yours? How about a
horseshoe multipolar like mine?

I have a protocol for PAS which you may have for the asking, but it
is to be found in any version of Pearse, A.G.E. and many others for the
looking. And by elaborating as I have done, I remain open to further
questions that will be welcome.

Regards,

Fred Monson
Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: Geoff McAuliffe
} Sent: Tuesday, July 10, 2001 10:47 AM
} To: CachatF-at-aol.com
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: stereology on thick sections
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} "CachatF-at-aol.com"-at-sparc5.microscopy.com wrote:
}
} } I am interested in counting the total number of glomeruli in the kidneys
} in
} } } rats with obstructive uropathy. To perform stereology, I have to cut
} thick
} } } sections at 20 micrometers. I found that the kidney itself breaks down
} and
} } } get very damaged at this thickness (kidneys fixed in 10% formalin and
} } } embedded in parrafin), probably because the kidney is much harder than
} the
} } } paraffin. In the litterature, some people embed kidneys in glycol
} } } methacrylate. It might be better (harder), but then I do not know how
} to
} } cut
} } } my sections which are about 2.5 to 3 cm long, if I need a
} glass/diamond
} } knife
} } } that long. Any ideas about cutting thick sections in paraffin?, or is
} it
} } } worth re-embedding in glycolmethacrylate, and any ideas about cutting
} my
} } } serial sections at 20 micrometers in that resin?
} } }
} } } Thank you a lot for your help!
} } }
} } } F. Cachat
} } } University of Virginia
} } } fc6b-at-virginia.edu
}
} How about frozen sections? Fix in formalin, cryoprotect with ascending
} concentrations of sucrose (10%, 20%, 30%) then snap freeze and section at
} 20
} microns on a sliding microtome. Collect sections in ice-cube trays to keep
} them
} in order.
} In my experience, thick paraffin sections are much more difficult than
} thinner
} ones. Embedding tissue in methacrylates (and other plastics) is usually
} intended
} for thinner sections (1-3 microns).
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}
}
}


From daemon Wed Jul 11 11:25:07 2001



From: Michael B. Ferrari :      ferrari-at-uark.edu
Date: Wed, 11 Jul 2001 11:19:16 -0500
Subject: Postdoc positions

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Research Associate position(s) available immediately to study roles of
calcium dynamics during somite formation and myofibril assembly. Primary
training in real-time digital fluorescence microscopy (both wide-field and
confocal) using calcium tools, FP-tagged constructs, and FRET approaches.
Experience in protein biochemistry or molecular biology highly desirable.
Salaries commensurate with qualifications and experience, start date is
negotiable. Research and position descriptions can be found at
http://biology.uark.edu/ferrari. Research and resources at the University
of Missouri - Kansas City School of Biological Sciences can be reviewed at
http://sgi.bls.umkc.edu/research.html. Please send cover letter, CV, and
names of three references to either address below or, preferably, attach
via e-mail: ferrari-at-uark.edu. The University of Missouri-Kansas City is an
affirmative action/equal opportunity employer.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Michael B. Ferrari
Department of Biological Sciences
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-5349
http://biology.uark.edu/ferrari

*****Note address change effective Jan. 2002*****
School of Biological Sciences
University of Missouri - Kansas City
Kansas City, Missouri 64110-2499
Ph: 816-235-2247
Fax: 816-235-5595





From daemon Wed Jul 11 11:39:31 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 11 Jul 2001 09:11:51 -0700
Subject: Re: Science Camp

Contents Retrieved from Microscopy Listserver Archives
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} Peggy Bisher wrote:
}
} } One of the scientists here wants to give a demonstration involving
} } vortices and fluids. He has a few short demonstrations to show the
} } kids, but I told him I think he needs more to keep their interest and
} } to keep them busy. He has five groups of 6 children for about an
} } hour.
}
Hi, Peggy --
}
The Exploratorium, an excellent hands-on science museum in San Francisco,
sells manuals on building interactive demo equipment; do a web search. For
example, here's a description of their optics manual (from the MICRO
bibliography - URL below):
----------------------
Doherty, P. and Rathjen, D. 1995 The Magic Wand and other Bright
Experiments on Light and Color 125pp, 7.5x9", $10.95. ISBN 0-471-11515-0
John Wiley & Sons, NY
The Exploratorium in San Francisco is famous for its interactive
science exhibits. They have published a series of "science snackbooks"
that give adults detailed instructions on how to duplicate those exhibits
for classrooms or science museums. This one collects 25 easy-to-construct
optics demonstrations that investigate reflection, refraction,
polarization, and magnification. Many could be good science fair projects.
All grade levels. RECOMMENDED
----------------------

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Jul 11 15:00:20 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 11 Jul 2001 15:53:02 -0400
Subject: Golgi-Cox Method for wax embedding (brain)

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Hi all!

Is anyone familiar with the Golgi-Cox Method for LM of brain? Any
help would be appreciated! The method I was referred to uses
Amfix(?) to decolorise the background. Has anyone heard of this and
where can I order it? Or if anyone has a better method that has
worked for them, please let me know.

Thanks!
Peggy Sherwood
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed Jul 11 15:33:10 2001



From: Steven Verhey :      Steven.Verhey-at-cwu.edu
Date: Wed, 11 Jul 2001 13:27:45 -0700
Subject: SEM: CPD

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We've recent acquired a Bomar SPC-900/EX Critical Point Dryer, but of course it didn't come with an operator's manual. Does anyone out there have a manual for this instrument, and if so, would you be willing to send or fax me a copy? Is this company still in business?

Thanks in advance,

Steve

Steven Verhey, Ph.D
Assistant Professor
Department of Biology
Central Washington University
Ellensburg, WA 98926
509.963.3431 office
509.963.2730 fax
verheys-at-cwu.edu


From daemon Wed Jul 11 17:04:30 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 11 Jul 2001 16:49:16 -0500
Subject: RE: magnetic field shielding

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Hello Dee,

Unfortunately, good and cost effective may not be go together when speaking
of large area magnetic shielding. The best material for magnetic shielding
is known as Mu-Metal. This material is typically supplied fully annealed
which provides the most effective shielding. FWIW, If strained by forming,
it is common to re-anneal. This probably won't affect you if you simply
"wallpaper" with sheet stock using contact cement. I would bond joints well
(pop rivets, solder?).
The web site for a supplier of Mu-Metal is: http://www.advancemag.com/

A poor second choice might be fully annealed silicon steel used in
electrical transformers.

Regards,
Woody White
McDermott Technology, Inc.
---------------------------------------------------------------------

} -----Original Message-----
} From: Dee Breger [mailto:micro-at-ldeo.columbia.edu]
} Sent: Wednesday, July 11, 2001 11:07 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: magnetic field shielding
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi colleagues,
}
} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?
}
} Much obliged,
} Dee
}
}
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 845/365-8640
} F: 845/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)
}
}
}


From daemon Wed Jul 11 17:40:37 2001



From: Kevin W. Eliceiri :      eliceiri-at-facstaff.wisc.edu
Date: Wed, 11 Jul 2001 17:40:01 -0500
Subject: Re: Science Camp

Contents Retrieved from Microscopy Listserver Archives
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I remember receiving the same scam from the mail.
I am surprised that people can and will succumb to these things.
Oh well, stupid is as stupid does.

Earl
----- Original Message -----
} From: "Nicol Aitken" {nicol-at-semiconductor.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, July 11, 2001 7:05 AM


} } Peggy Bisher wrote:
} }
} } } One of the scientists here wants to give a demonstration involving
} } } vortices and fluids. He has a few short demonstrations to show the
} } } kids, but I told him I think he needs more to keep their interest and
} } } to keep them busy. He has five groups of 6 children for about an
} } } hour.
} }
} Hi, Peggy --
} }
} The Exploratorium, an excellent hands-on science museum in San Francisco,
} sells manuals on building interactive demo equipment; do a web search. For
} example, here's a description of their optics manual (from the MICRO
} bibliography - URL below):


I would like to second Caroline's recommendation for the exploratium.
They have put a lot of the interactive demos on their website,check
out: http://www.exploratorium.edu/snacks/snacksbysubject.html There
are other great informal science education tools on their website,
try using their search engine to find what you need.

best,
kevin


From daemon Wed Jul 11 19:12:56 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 11 Jul 2001 19:06:43 -0500
Subject: Re: SEM: CPD

Contents Retrieved from Microscopy Listserver Archives
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} Steve,

Bomar is a name that will live in infamy.....at least in this
facility. More than 15 yrs ago, the former director returned a CPD
unit to Bomar for repair. After not hearing from them for many
months, and after her phone calls were not returned, she demanded the
return of the unit. No response. The company had gone bankrupt, its
assets were seized and our unit was gone. We did file a lawsuit
against the company but were told to forget pursuing the matter since
it would cost more than the unit was worth. If you do find that they
are in business again, let me know. I will look around to see if any
information on the CPD.

Good luck......

JB



} We've recent acquired a Bomar SPC-900/EX Critical Point Dryer, but
} of course it didn't come with an operator's manual. Does anyone out
} there have a manual for this instrument, and if so, would you be
} willing to send or fax me a copy? Is this company still in business?
}
} Thanks in advance,
}
} Steve
}
} Steven Verhey, Ph.D
} Assistant Professor
} Department of Biology
} Central Washington University
} Ellensburg, WA 98926
} 509.963.3431 office
} 509.963.2730 fax
} verheys-at-cwu.edu

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Jul 11 19:18:28 2001



From: Smith, Peter :      peter.smith-at-agresearch.co.nz
Date: Thu, 12 Jul 2001 12:13:58 +1200
Subject: Re stereology on thick sections

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We routinely cut 20-50 micron sections from methacrylate blocks for
stereology and as far as we are concerned it is the only way to go for
stereology studies. We use technovit 7100 kits and deal with samples as
large as whole ovaries from sheep.If the whole ovary is to large we simply
cut it in half and process and section seperately, for stereology studies we
simply count the 2 halves seperately and the sum of the 2 halves = the whole
organ.The cutting is a bit of an art but with practice becomes fairly easy,
fortuneately we are blessed here with an artist and the following is a
description of how she does the cutting.We have also used a number of
different stains on the thick sections, if you decide to go down this path
and need any further info don't hesitate to e-mail us, alternatively look
for any references by HJG Gundersen, in my opinion the guru of stereology, a
good review article is in APMIS 96:1988 p 379-394 and p857-881.


The following is how we cut thick sections (30 Microns), using Technovit
Resin.
Before cutting thick sections, blocks have to be completely hard, either
been made for months or hardened in a 37 degree oven for 3-5 days. Unlike
paraffin sections which have a right and wrong side, Resin/Plastic sections
can be placed in the waterbath on either side, so for this reason I cut the
right hand corner off the block, so I can regulate the mounting of sections
onto slides.
I use a Microm Rotary Microtome HM 350 with a Ralph Glass Knife with a
concave knife profile, knives are made on a LKB Histo Knifemaker. The
microtome is programmed with a cutting window (selected according to the
size of the block) and a cutting speed (selected speed is 2.2). As I mainly
do serial sections I have a continuous stroke programmed on the microtome
(this means that I pick up a section every 5-10seconds), the microtome is
controlled with the foot/hand switch.
My waterbath is a large glass staining dish with distilled water at room
temperature which is placed in a black box (so that you can see the sections
on the bottom of the waterbath). I also have a small glass beaker with
distilled water and a pipette, this is so that I can put water onto the
glass knife as the section is being cut. Cut sections are taken to forceps
and placed into the waterbath were they rest on the bottom of the waterbath
until they are picked up onto cleaned glass slides and placed into a 60
degree oven for at least 30 minutes (sections are very wrinkled at first but
straighten out with the heat from the oven).

Hope this is of some help

Peter Smith Reproduction Group
AgResearch Wallaceville Research Centre
P.O. Box 40063, Upper Hutt, New Zealand

Tel +64 04 9221 309 Fax +64 04 9221 380
e-mail peter.smith-at-agresearch.co.nz



========================================================================
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The information contained in this message and or attachments is intended
only for the person or entity to which it is addressed and may contain
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this information by persons or entities other than the intended recipient
is prohibited. If you have received this message in error, please notify
the sender immediately.


From daemon Thu Jul 12 01:43:01 2001



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Thu, 12 Jul 2001 08:35:03 +0200
Subject: Re: Golgi-Cox Method for wax embedding (brain)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am not familiar with the Golgi-Cox method, but if it involves silver
in any shape or form, then the "Amfix" most probably refers to a
photographic rapid fixer (ammonium thiosulphate). As far as I know
this is still available, but Agfa Agefix and Ilford Hypam would be
equivalent. Kodak Unifix would probably work as well, but it does
contain a photographic emulsion hardener.


Jan Coetzee

Peggy Sherwood wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all!
}
} Is anyone familiar with the Golgi-Cox Method for LM of brain? Any
} help would be appreciated! The method I was referred to uses
} Amfix(?) to decolorise the background. Has anyone heard of this and
} where can I order it? Or if anyone has a better method that has
} worked for them, please let me know.
}
} Thanks!
} Peggy Sherwood
} --
} Peggy Sherwood
} Lab Associate, Photopathology
} Wellman Laboratories of Photomedicine (W224)
} Massachusetts General Hospital
} 50 Blossom Street
} Boston, MA 02114
} 617-724-4839 (voice mail)
} 617-726-6983 (lab)
} 617-726-3192 (fax)
} sherwood-at-helix.mgh.harvard.edu

--
Prof Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


From daemon Thu Jul 12 03:24:16 2001



From: j.bilde-at-risoe.dk
Date: Thu, 12 Jul 2001 10:16:36 +0200
Subject: http://www.spicerconsulting.com/sc12.htmRe: magnetic field shield ing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dee Breger wrote:
Can anyone recommend a good/cost-effective type of wall shielding to
eliminate 60 and 180 cycle interferences coming into an SEM room from
recently-installed large motors on the opposite side of the wall (that
can't be moved now)?

Hi,
Have you as an alternative considered to use the magnetic field cancellation
systems that are available nowadays? The first I found by searching the web
for "magnetic field cancellation" was:

http://www.spicerconsulting.com/sc12.htm

but there are quite a few other producers.

Best regards,
Jorgen.

{:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm




From daemon Thu Jul 12 03:34:40 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 11 Jul 2001 16:49:16 -0500
Subject: RE: magnetic field shielding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am too confront with this problem. But we can move the SEM (witch we
have just moved... in a electromagnetic "dirty" room!).

As Woody said it, mu-metal is the best way, but with terrible difficulties
and cost. One of our collegues, magnetism specialist, proposed to use
thick aluminium or cooper plates (10 mm or more thick). Aluminium (dural)
has the big advantage to be light, copper is better for electrical
continuity, but heavier and expensive (with 10 to 15 mm thick...), soft
iron or silicon iron is not too expensive, but heavy. You must build a box
around the SEM, with a door on entry lock side and a good electrical
continuity. It looks nice ! It's not a simple problem, but as
consolation, remember that you can recycle the plates when the
interferences are eliminated. But of course the best way is to eliminate
the source of interferences.

By the way, did you mesure the AC field ? In case, how much do you have ?
I am interested on the solution you will find. Our building is VERY noisy.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

---------- Forwarded message ----------


Hello Dee,

Unfortunately, good and cost effective may not be go together when speaking
of large area magnetic shielding. The best material for magnetic shielding
is known as Mu-Metal. This material is typically supplied fully annealed
which provides the most effective shielding. FWIW, If strained by forming,
it is common to re-anneal. This probably won't affect you if you simply
"wallpaper" with sheet stock using contact cement. I would bond joints well
(pop rivets, solder?).
The web site for a supplier of Mu-Metal is: http://www.advancemag.com/

A poor second choice might be fully annealed silicon steel used in
electrical transformers.

Regards,
Woody White
McDermott Technology, Inc.
---------------------------------------------------------------------

} -----Original Message-----
} From: Dee Breger [mailto:micro-at-ldeo.columbia.edu]
} Sent: Wednesday, July 11, 2001 11:07 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: magnetic field shielding
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi colleagues,
}
} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?
}
} Much obliged,
} Dee
}
}
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 845/365-8640
} F: 845/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)
}
}
}



From daemon Thu Jul 12 08:31:04 2001



From: hard-at-acsu.buffalo.edu
Date: Thu, 12 Jul 2001 09:26:54 -0500
Subject: COURSE ANNOUNCEMENT (2nd Posting)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 10 - October 19, 2001

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: July 25, 2001

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual

research problems with the faculty.



From daemon Thu Jul 12 08:31:04 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Thu, 12 Jul 2001 09:22:05 -0400 (EDT)
Subject: used microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Lister,

I am still looking for a used, better or alike Ultracut E ultramicrotome.
Thank you very much for your help.

Haixin Xu

BS/UMBC
Baltimore, MD 21228
410-455-2296



From daemon Thu Jul 12 09:06:31 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 12 Jul 2001 09:57:34 -0400
Subject: Re: Golgi-Cox Method for wax embedding (brain)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 3:53 PM -0400 7/11/01, Peggy Sherwood wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all!
}
} Is anyone familiar with the Golgi-Cox Method for LM of brain? Any
} help would be appreciated! The method I was referred to uses
} Amfix(?) to decolorise the background. Has anyone heard of this and
} where can I order it? Or if anyone has a better method that has
} worked for them, please let me know.
}
} Thanks!
} Peggy Sherwood
} --
*****************

Hi Peggy,
Here is the Golgi-Cox protocol, with formulae. Its out
of:"Histological & Histochemical Methods: Theory & Practice" by JA
Kiernan (1981)Pergamon Press.

A. Golgi-Cox Fixative-prepared from 3 stable stock solutions:
(i) 5% aqueous mercuric chloride (HgCl2)
(ii) 5% aqueous potassium dichromate (K2Cr2O7)
(iii)5% aqueous potassium chromate (K2CrO4)

Working s olution} Mix, IN ORDER
(i) 20ml
(ii) 20ML
Water: 40ml
(iii) 16ml
Mix before useing.

B. Alkaline developer
Strong ammonia solution:
(17% NH3) 5 ml
Water 95 ML
Mix just before using.

Procedure:

1. fix pieces of nervous tissue (not more than 10mm thick) in
the working sol'n of the golgi-Cox fix for 6-8 weeks in a tightly
capped container at 37 deg. C. Pour off fix and replace with fresh
after first day.
2. Wash in water, many changes, for 6-8 hours, then dehydrate
and embed in nitrocellulos. Cut sections at 100 microns thick.
3. treat sections with the alkaline developer (sol'n B) for 2-3 minutes.
4. Wash in water, dehydrate, clear & mount in a resinous
medium. (see NOTE)

Result: some neurons & neuroglial cells, including cytoplasmic
processes--black. Background, pale yellow.

NOTE: A mixture of 33ml chloroform, 33ml xylene and 33ml abs,.
alcohol is preferred as the lat stage of dehydration. This can be
followed by clearing for 10-15 min in creosote or in cedarwood oil,
followed by a rinse in xylene.

thae black color fades with time if a coverslip is applied, so it is
usual to mount sections in THICK canada Balsam, and allow to set at
40-45 deg C without a coverslip. Such preps. are stable for many
years. If a synthetic mounting medium is used (such as DPX),
coverslips may be applied, but fading occurs after about 6 months.


Its a lot of work, using reagents most of us are not familiar with,
and which will make your Health & Safety officers cringe! good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Jul 12 09:48:50 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 12 Jul 2001 09:42:15 -0500
Subject: RE: HMDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Thanks All ... for your cautions and info regarding HMDS. It
} gives good results, but I will definately use a hood or go
} back to my CPD!


HMDS is much more convenient than a CPD, and for my specimens it
gives mostly results. Hood is a minor inconvenience.

Vladimir


From daemon Thu Jul 12 10:26:16 2001



From: Richard R. Vanfleet :      vanfleet-at-physics.ucf.edu
Date: Thu, 12 Jul 2001 11:22:31 -0400
Subject: Philips 400 series hot stage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a Philips 400 series hot stage. The intent is to make
some modifications to it so that even one that is broken (depending upon
what is broken) might be acceptable. If you have one that you might be
willing to part with, please communicate to me its state and under what
conditions you might let it go.

Richard

Richard Vanfleet
Assistant Professor
Advanced Materials Processing and Analysis Center (AMPAC)
and Department of Physics

Mail:
AMPAC
University of Central Florida
12443 Research Parkway, Suite 404
Orlando, FL 32826

Phone: (407)882-1460
FAX: (407)882-1462
vanfleet-at-physics.ucf.edu



From daemon Thu Jul 12 10:37:16 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 12 Jul 2001 16:40:50 +0100
Subject: Strange lines on scanned negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

I thought this might be worth drawing to your attention if you scan e.m.
negatives in with a flat bed scanner.

We currently use an Epson Perfection 1200 with transparency adapter. I
know the spec is considered to be pretty low in terms of O.D., greyscale
and DPI but it produces very good pictures about 98% of the time. One
problem that I have encountered, however, from time to time is one or
rarely more than one extremely fine dark lines appears on the scanned
image. This can be very annoying because they are only visible if you
look very carefully at an enlarged image. I had assumed that there was a
problem with one or two points on the CCD scanner detector array because
the lines were straight and at right angles to the scanner head.

The lines are actually caused because this particular scanner has a
small reference slot to calibrate the CCDs for fluctuations in the
light. If a small particle of dust falls in this area of the glass top
it causes the the scanner to overcompensate for the CCD on the
corresponding point of the scanner head. This usually results in a very
fine white line on the negative image or black on the positive image. I
confirmed this using a series of varying density dots on film and got a
perfect series of lines.

I'm sorry if people knew this already but thought it just might save
someone tearing their hair out or throwing a perfectly functional
scanner away. I don't know if some of the other flatbed glass topped
scanners with transmitted light sources use a similar calibration system
but it's worth taking extra care if they do.

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
SUNDERLAND
UK


From daemon Thu Jul 12 11:36:22 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 12 Jul 2001 09:30:22 -0700 (PDT)
Subject: Re: more on that scam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One other very interesting aspect of the scam. The letters sent via
snailmail used counterfeit (forged) stamps. So many of these letters were
sent that copies of these fakes still show up in packets of stamps sold to
collectors. And the envelopes with stamps on them (called covers in the
hobby) are now considered to be relatively valuable collectors' items. (Oh,
you mean it's obvious that I'm one of those stamp collectors? Too true.)

Roger
On Wed, 11 Jul 2001 15:38:24 -0700, Earl Weltmer wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| I remember receiving the same scam from the mail.
| I am surprised that people can and will succumb to these things.
| Oh well, stupid is as stupid does.
|
| Earl
| ----- Original Message -----
| } From: "Nicol Aitken" {nicol-at-semiconductor.com}
| To: {Microscopy-at-sparc5.microscopy.com}
| Sent: Wednesday, July 11, 2001 7:05 AM
| Subject: RE: more on that scam
|
|
| }
------------------------------------------------------------------------
| } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
| } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| }
-----------------------------------------------------------------------.
| }
| }
| }
| }
| }
| } } Hello listers,
| } } I recall seeing that thread late last week about the business
| opportunity.
| } } This just came in from a Canadian news source, CBC.
| } } Nick
| } } RCMP make arrests in international phone scam
| } } WebPosted Tue Jul 10 17:27:29 2001
| } } TORONTO - The RCMP, along with the FBI and the U.S. Secret Service,
have
| } } cracked a multi-million dollar scam originating in Nigeria and
arrested
| } } three people in the Toronto area.
| } } More than 300 people around the world - most of them Americans - were
| } } duped into giving money to a fake organization. The RCMP say some
people
| } } lost more than $50,000. Others lost millions.
| } } Police say the fraud was sophisticated, high tech and organized.
| } } Typically, victims received a letter either by mail or fax allegedly
| from
| } } officials of a large Nigerian institution, such as the Nigerian
National
| } } Petroleum Corporation or the Central Bank of Nigeria.
| } } The letters would contain an urgent request for help in transferring
| } } millions of dollars out of Nigeria to financial institutions in the
| } } victim's country. If the victims would help, they'd be in line for a
| } } handsome profit, in some cases as much as 30 per cent of the monies
| being
| } } transferred.
| } } The letter says foreign accounts are needed and victims were asked to
| wire
| } } personal banking information and $10,000 US to cover administration
| fees.
| } } 'Boiler room' in Toronto
| } } Then, victims were contacted by a person posing as a merchant banker
of
| } } the Central Bank of Nigeria. They were told the funds were now in a
| } } clearing house or mercantile bank in North America.
| } } Phone numbers given for the fake banks actually rang in a "boiler
room"
| in
| } } the Toronto area.
| } } The scam continued with the victim asked to pay taxes, duties and
other
| } } fees, all with the promise of a windfall, until the victim was
bankrupt.
| } } The RCMP began a co-ordinated effort with U.S. police forces in 1998.
| } } According to an RCMP news release, police detachments in the Toronto
| area
| } } have received at least one complaint a week for the past eight years
| from
| } } victims who lost money in the scam.
| } } Staff Sergeant Darryl Ross says victims lost more than money. Some
| } } suffered health problems, mental breakdowns, divorce and the break-up
of
| } } their families.
| } }
| } }
| }
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Thu Jul 12 13:18:09 2001



From: Kalman Rubinson :      kr4-at-nyu.edu
Date: Thu, 12 Jul 2001 14:13:37 -0400
Subject: Re: Golgi-Cox Method for wax embedding (brain)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Peggy Sherwood wrote:

} } Is anyone familiar with the Golgi-Cox Method for LM of brain? Any
} } help would be appreciated! The method I was referred to uses
} } Amfix(?) to decolorise the background. Has anyone heard of this and
} } where can I order it? Or if anyone has a better method that has
} } worked for them, please let me know.

I am not familiar with any need to decolorize the background in most of
the Golgi impregnation methods, the Cox variant included. OTOH, my
experience
has not been with wax embedding but only with the harder (celloidin,
araldite,
etc.) media do not require any clearing.

There is, however, a need to add an oxident, like NaOH, to the
alkalinization
step after impregnation and before embedding to prevent background
blackening.

Kal Rubinson


From daemon Thu Jul 12 13:42:54 2001



From: Phoebe J Doss/app/Cvm :      pjdoss-at-cvm.okstate.edu
Date: Thu, 12 Jul 2001 13:37:48 -0500
Subject: Sorvall JB4 microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I have a Sorvall JB4 microtome that is not advancing at all. If anyone has
any suggestions, please contact me. Thanks.

Phoebe

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Department of Physiological Sciences
264 McElroy Hall
Oklahoma State University
Stillwater, OK 74078
405-744-6765



From daemon Thu Jul 12 13:52:28 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 12 Jul 2001 13:53:08 -0500
Subject: Re: magnetic field shielding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My two cent's worth: as a former Spicer Consulting SC12 user, I was highly
satisfied with the unit. We too had EMI from large power cables for water pumps
and some other unknown source affecting our SEM images and the SC12 cleared it
up. I don't remember how much the unit cost, but remember that is was less than
a room full of Mu-Metal. With the added advantages of easy installation and if
the SEM ever changes locations, the EMI shielding (the unit) can go with it.

"j.bilde-at-risoe.dk"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dee Breger wrote:
} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?
}
} Hi,
} Have you as an alternative considered to use the magnetic field cancellation
} systems that are available nowadays? The first I found by searching the web
} for "magnetic field cancellation" was:
}
} http://www.spicerconsulting.com/sc12.htm
}
} but there are quite a few other producers.
}
} Best regards,
} Jorgen.
}
} {:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::} ¤ {:::}
}
} Joergen B. Bilde-Soerensen
} Senior Research Scientist, Ph. D.
} Materials Research Department
} Risoe National Laboratory
} DK-4000 Roskilde
} Denmark
}
} e-mail: j.bilde-at-risoe.dk
} phone: +45 4677 5802 (direct)
} phone: +45 4677 4677 (switchboard)
} fax: +45 4677 5758
} website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
DSPS Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Jul 12 14:47:31 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 12 Jul 2001 14:32:31 -0500
Subject: "Electronic eyepiece"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Any opinions of this $195 device?
http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=1164152801

- John
P.S. I have no association with this auction.



From daemon Thu Jul 12 14:47:32 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Thu, 12 Jul 2001 14:36:56 -0700
Subject: Re: magnetic field shielding (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am intrigued by the recommendation of the referenced magnetism
specialist to use aluminum and/or copper plate. The principle in using
high conductivity non ferromagnetic shielding (such as Al and Cu) is
that of eddy current shielding. This is very effective for high
frequency magnetic fields, but becomes worthless as you approach DC
(except for the special case of super conducting material, which does
shield DC fields). Line-frequency magnetic fields (50-60Hz) are
typically dealt with via ferromagnetic shielding because of the
relatively low frequency.

Presumably your specialist has done the calculations and feels that eddy
current shielding will do the job. This suggests one of three things to
me: (1) he knows the problems are due to relatively high frequencies;
(2) your fields are very severe and he is only trying to knock them down
to a more tractable level; or (3) he knows something I don't. I would
personally be interested in learning more about how he arrived at this
solution. Specifically, what level of attenuation does he anticipate
achieving for 50 Hz magnetic fields with this kind of shielding?

Fred Schamber

Faerber Jacques wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am too confront with this problem. But we can move the SEM (witch we
} have just moved... in a electromagnetic "dirty" room!).
}
} As Woody said it, mu-metal is the best way, but with terrible difficulties
} and cost. One of our collegues, magnetism specialist, proposed to use
} thick aluminium or cooper plates (10 mm or more thick). Aluminium (dural)
} has the big advantage to be light, copper is better for electrical
} continuity, but heavier and expensive (with 10 to 15 mm thick...), soft
} iron or silicon iron is not too expensive, but heavy. You must build a box
} around the SEM, with a door on entry lock side and a good electrical
} continuity. It looks nice ! It's not a simple problem, but as
} consolation, remember that you can recycle the plates when the
} interferences are eliminated. But of course the best way is to eliminate
} the source of interferences.
}
} By the way, did you mesure the AC field ? In case, how much do you have ?
} I am interested on the solution you will find. Our building is VERY noisy.
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
} ---------- Forwarded message ----------
} Date: Wed, 11 Jul 2001 16:49:16 -0500
} } From: "White, Woody N." {nwwhite-at-mcdermott.com}
} To: 'Dee Breger' {micro-at-ldeo.columbia.edu} , microscopy-at-sparc5.microscopy.com
} Subject: RE: magnetic field shielding
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Dee,
}
} Unfortunately, good and cost effective may not be go together when speaking
} of large area magnetic shielding. The best material for magnetic shielding
} is known as Mu-Metal. This material is typically supplied fully annealed
} which provides the most effective shielding. FWIW, If strained by forming,
} it is common to re-anneal. This probably won't affect you if you simply
} "wallpaper" with sheet stock using contact cement. I would bond joints well
} (pop rivets, solder?).
} The web site for a supplier of Mu-Metal is: http://www.advancemag.com/
}
} A poor second choice might be fully annealed silicon steel used in
} electrical transformers.
}
} Regards,
} Woody White
} McDermott Technology, Inc.
} ---------------------------------------------------------------------
}
} } -----Original Message-----
} } From: Dee Breger [mailto:micro-at-ldeo.columbia.edu]
} } Sent: Wednesday, July 11, 2001 11:07 AM
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: magnetic field shielding
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } Hi colleagues,
} }
} } Can anyone recommend a good/cost-effective type of wall shielding to
} } eliminate 60 and 180 cycle interferences coming into an SEM room from
} } recently-installed large motors on the opposite side of the wall (that
} } can't be moved now)?
} }
} } Much obliged,
} } Dee
} }
} }
} }
} }
} } ***************************************************************
} } Dee Breger
} } Mgr. SEM/EDX Facility
} } Lamont-Doherty Earth Observatory
} } 61 Route 9W
} } Palisades, NY 10964 USA
} } T: 845/365-8640
} } F: 845/365-8155
} }
} } http://www.ldeo.columbia.edu/micro
} } http://www.discovery.com/area/science/micro/micro1.html
} } http://www.lsc.org/antarctica/front.html
} } Journeys in Microspace (Columbia University Press, 1995)
} }
} }
} }


From daemon Thu Jul 12 15:41:02 2001



From: Julian Martinez-Fernandez :      Julian.Martinez-Fernandez-at-grc.nasa.gov
Date: Thu, 12 Jul 2001 16:30:19 -0400
Subject: Supplier of negative films for the Philips CM-200 EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleges:

Our supplier of negative films (Agfa) for the Philips CM-200 EM in
the Microscopy Service of the University of Seville does not fabricate
these negatives any more. According to Philips Spain, Kodak does not sell
those negatives in Spain (Kodak Electron Microscopy 4489, Dimensions: 6.5 x
9 cm) and they do not give us an immediate solution (?).

Could somebody help us with this silly problem and let us
know of a supplier close to Spain?

Many thanks,
Julian



From daemon Thu Jul 12 15:44:00 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 12 Jul 2001 13:38:31 -0700
Subject: RE: magnetic field shielding (fwd)

Contents Retrieved from Microscopy Listserver Archives
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May be I am wrong, but aluminum is "transparent" to the magnetic fields (as
well as copper). Since the physics course in the middle school, I had
strong feelings that soft iron is a good material for shielding. In Russia
we had special requirements for EM rooms (like "floating" basement and
proper magnetic/electric shielding). As I remember, our EM room has iron
mesh immersed into the walls/ceilings/floors. That mesh was properly
grounded (copper sheet 1x1 m buried 5 meters deep into the ground, 20
meters away of the building with very thick copper "conductor"; this
"conductor" was mounted across the perimeter of the room and was connected
to the mesh at many points). It also seems to me that the thickness of
shielding material do not so important. I think, described "shielding"
schedule was designed mostly against electric fields, but it should works
against magnetic fields as well. I would be happy to listen the comments of
the specialist on this matter.

Sergey.

At 10:29 AM 7/12/01 +0200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jul 12 22:22:23 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 13 Jul 2001 15:20:48 GMT+1200
Subject: Re: "Electronic eyepiece"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I want one, too.

Does anyone know who makes them and/or sells them on a regular basis?


rtch


}
}
}
} Any opinions of this $195 device?
} http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=1164152801
}
} - John
} P.S. I have no association with this auction.
}
}


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Jul 13 07:26:48 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 13 Jul 2001 22:17:10 +1000
Subject: RE: Supplier of negative films for the Philips CM-200 EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Its the Kodak dealer that has chosen not to stock that size. We no longer stock
boxes of 100 sheets because there was too little demand and its very expensive
when film goes out of date. ProSciTech and half a dozen other EM suppliers
would be delighted to supply you. Just ask for a quote including freight.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, July 13, 2001 6:30 AM, Julian Martinez-Fernandez
[SMTP:Julian.Martinez-Fernandez-at-grc.nasa.gov] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear colleges:
}
} Our supplier of negative films (Agfa) for the Philips CM-200 EM in
} the Microscopy Service of the University of Seville does not fabricate
} these negatives any more. According to Philips Spain, Kodak does not sell
} those negatives in Spain (Kodak Electron Microscopy 4489, Dimensions: 6.5 x
} 9 cm) and they do not give us an immediate solution (?).
}
} Could somebody help us with this silly problem and let us
} know of a supplier close to Spain?
}
} Many thanks,
} Julian



From daemon Fri Jul 13 07:26:48 2001



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Fri, 13 Jul 2001 13:53:47 +0200
Subject: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied to my question about the DTSA software package. I
have downloaded it onto a Mac here. As yet, I don't have it fully working
as it doesn't want to import the EMSA files exported by our Noran Voyager
system. I'm still trying to working out what the problem is, but in
principle it seems to be working (except with my files).

As helpfully pointed out by Scott Wight at NIST, the latest version resides
on their server at
http://www.cstl.nist.gov/div837/Division/outputs/DTSA/DTSA.htm

Thanks again

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/



From daemon Fri Jul 13 08:02:49 2001



From: =?iso-8859-1?Q?=22Dr=2E_Jos=E9_Luis_Pech=2DPacheco=22?= :      pech-at-optica.csic.es
Date: Fri, 13 Jul 2001 07:59:21 -0500
Subject: deep focus of objetives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need information about the deep focus of objetives:

Plan Neofluar, 2.5/0.075, inf/0.17

Ph1 Plan Neofluar, 10x/0.30,inf/0.17

Ph2 Plan neofluar, 20x/0.50,inf/0.17

Ph2 plan neofluar, 40x/0.75, inf/0.17

thank you for your help


___________________________________

Dr. José Luis Pech Pacheco
PhD. Marine Ecology
Image & Vision D.
Instituto de Óptica, CSIC.
Madrid, Spain
Fax 0034915645557
_____________________________________


From daemon Fri Jul 13 08:20:42 2001



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Fri, 13 Jul 2001 22:15:29 +0900
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Fri Jul 13 08:25:21 2001



From: gongw :      gongw-at-cua.edu
Date: Fri, 13 Jul 2001 09:20:19 -0400
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please subscribe!

Thanks



From daemon Fri Jul 13 09:08:58 2001



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 13 Jul 2001 10:04:30 -0500
Subject: Re: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Thanks to all who replied to my question about the DTSA software package. I
} have downloaded it onto a Mac here. As yet, I don't have it fully working
} as it doesn't want to import the EMSA files exported by our Noran Voyager
} system. I'm still trying to working out what the problem is, but in
} principle it seems to be working (except with my files).

FYI, there are two DTSA plug-ins for EMSA import. A version 1.0 and version
1.1. Check to make sure your are using the correct one. If you still have
problems, You can also e-mail me some of your Voyager EMSA files and I can
tell you what is the problem. I'm well versed with the internals of DTSA
and that of EMSA file formats.

Scott

-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Fri Jul 13 10:05:57 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Fri, 13 Jul 2001 10:41:48 -0500
Subject: A New Product

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,
I just received a product release on a new product, one that I had not heard
of before, that may be of interest to a number of you.
The product, called ScanScope T50, from Aperio Technologies converts all
information from a microscope slide into a common digital format. They say,
for example, that a typical tissue section can be digitized at 54,000 pixels
per inch.
Their web site is www.aperiotech.com
Obvioulsy I (unfortunately)have no financial or other interests in this
company or its products.
Don Grimes, Microscopy Today



From daemon Fri Jul 13 10:41:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 13 Jul 2001 11:34:02 -0400
Subject: "Electronic eyepiece"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had seen this item a while back and may have said something to the listserver. At the time, I thought that it would have worked nicely on the eyepiece on a microprobe and perhaps on the binoculars of a TEM. I had gotten some information on it and here is the contact information:

International Micro-Vision Inc.
667 El Camino Real
Redwood City, CA
94063-1317

P: (650) 299-9794
F: (650) 366-7760
Email: david-at-imicrovision.com
URL: http://www.imicrovision.com



-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: John Foust [mailto:jfoust-at-threedee.com]
Sent: Thursday, July 12, 2001 3:33 PM
To: microscopy-at-sparc5.microscopy.com



Any opinions of this $195 device?
http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=1164152801

- John
P.S. I have no association with this auction.



From daemon Fri Jul 13 10:43:05 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 13 Jul 2001 10:34:30 -0500
Subject: Re: A New Product

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I went to the web site. Not very impressive. Their big product
digitizes all the info on a slide without a conventional microscope.
OK, nice idea but don't you think they could have posted at least one
image! No data of any type at the site.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Jul 13 10:43:12 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 13 Jul 2001 11:40:17 -0400
Subject: RE: magnetic field shielding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dee,

I have not solved our problems completely but will share some things I have
"heard". If you decide to go with the mu-metal, it should be easier and
cheaper to build a small enclosure around the actual source (motors) than to
shield the EM or entire room. This assumes you are confident that the new
motors are the source; i.e., the SEM was fine before their installation.
Anyway, this is how I was advised to proceed; unfortunately we could not
pinpoint a single source, so I haven't actually tried it. Often, the advice
you may recieve from "magnetism experts" and shielded-room experts is better
suited to the Earth's steady field than it is to A/C fields.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Wednesday, July 11, 2001 11:07 AM, Dee Breger [SMTP:micro-at-ldeo.columbia.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi colleagues,
}
} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?
}
} Much obliged,
} Dee
}
}
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 845/365-8640
} F: 845/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)
}



From daemon Fri Jul 13 10:59:48 2001



From: NPGSlithography-at-aol.com
Date: Fri, 13 Jul 2001 11:54:43 EDT
Subject: Re: magnetic field shielding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 7/11/2001 3:14:47 PM Mountain Daylight Time,
micro-at-ldeo.columbia.edu writes:

} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?

Can you be more specific on what you mean by cost-effective by providing an
actual dollar amount that you could afford? Otherwise, many of the
suggestions being made may be completely impractical based on funding
concerns.

The reason I suggest this is that the last I heard, the commercial magnetic
field canceling systems cost from ~$10k to ~$25k. Also, one person has said
that covering a room in mu-metal may be of a similar cost.

A less expensive mu-metal solution may be to build a box around the column,
or even just around the chamber. Years ago, JEOL sold such a mu-metal box
that surrounded just the chamber of a 840A SEM (on 4 full sides plus the top
and bottom where it stopped at the perimeter of the column and chamber,
respectively). At the time, it cost about $6k from JEOL. Using the JEOL
mu-metal box (which was about 2mm thick, if I recall correctly), reduced the
interference on the beam by about a factor of 3. (We were doing lithography
at the time, so it was easy to compare the amplitude of the interference in
the patterns that were written.)

I know one person who designed a similar box at a substantially lower cost
using an in-house machine shop. The key in that case is to properly anneal
the mu-metal after it has been fabricated.

Regarding the idea of using Cu or Al, I agree with the previous postings that
question its usefulness for line frequency magnetic fields, although I
haven't done any calculations to evaluate the suggestion.

I have also heard of soft iron being used, which should be relatively
inexpensive, but will be very heavy because of the increased thickness that
would be required to match the shielding of mu-metal.

Could you build a heavy, ugly, and cheap box around the motors???

The lowest cost solution may be to move the SEM, if you have a suitable room
available. A professional move may cost thousands, but doing it yourself is
a reasonable option, if you know about shipping bolts and scintillators.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Fri Jul 13 11:01:51 2001



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Fri, 13 Jul 2001 12:01:34 -0400
Subject: Re: Supplier of negative films for the Philips CM-200 EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try mandel Scientific , Canada if you cannot find it closer.
e-mail info-at-mandel.ca



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Julian Martinez-Fernandez {Julian.Martinez-Fernandez-at-grc.nasa.gov} 07/12 4:30 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear colleges:

Our supplier of negative films (Agfa) for the Philips CM-200 EM in
the Microscopy Service of the University of Seville does not fabricate
these negatives any more. According to Philips Spain, Kodak does not sell
those negatives in Spain (Kodak Electron Microscopy 4489, Dimensions: 6.5 x
9 cm) and they do not give us an immediate solution (?).

Could somebody help us with this silly problem and let us
know of a supplier close to Spain?

Many thanks,
Julian





From daemon Fri Jul 13 11:06:53 2001



From: DrJohnRuss-at-aol.com
Date: Fri, 13 Jul 2001 12:01:48 EDT
Subject: Re: A New Product

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 7/13/01 10:55:26 AM, PhillipsT-at-missouri.edu writes:

} I went to the web site. Not very impressive. Their big product
} digitizes all the info on a slide without a conventional microscope.
} OK, nice idea but don't you think they could have posted at least one
} image! No data of any type at the site.

I agree that there is no info whatever on the site. Also, note that 54000
points per inch is 1/2 micron. Certainly that is not real resolution, and one
wonders how with anything approaching that optical resolution they can assure
good focus. It takes some serious mechanical engineering and software to scan
a whole slide to collect and tile images. I've seen it done, using good
microscopes and stages with well designed optics and a digital camera. I'd
have to see some real examples before believing anything from this outfit.


From daemon Fri Jul 13 15:00:43 2001



From: Metaxas, Constantine (NAP) :      metaxac-at-bp.com
Date: Fri, 13 Jul 2001 14:52:59 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Costas Metaxas, Ph.D.
(630) 420-4842
(630) 420-4507 FAX
metaxac-at-bp.com



From daemon Fri Jul 13 15:58:45 2001



From: Hay Randall S Civ AFRL/MLLN :      Randall.Hay-at-wpafb.af.mil
Date: Fri, 13 Jul 2001 16:45:24 -0400
Subject: unsubscribe

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unsubscribe


From daemon Sat Jul 14 06:34:03 2001



From: m.andersson-at-t-online.de (Maike Andersson)
Date: Sat, 14 Jul 2001 13:49:36 +0200
Subject: LM-Fluorescence

Contents Retrieved from Microscopy Listserver Archives
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I am a newcomer to this field, trying to differentiate between
autofluorescent
lignin, callose, cutin and suberin in LR-white embedded plant material.
My question is:
Is there a way of identifying one of these substances by selectively
quenching
the autofluoescence of the others ?
Can the colour of the emission be used as a tool for differentiation ?
I tried Berberinsulfat but it stains lignin and suberin. Neutralred stains
the
hydrophobic domain of suberin but is also used as a fluorochrome for lipids
in general and therefore probably stains cutin as well.

Any help will be appeciated.
Maike Andersson




From daemon Sat Jul 14 15:17:11 2001



From: tcote-at-medicacorp.com ()
Date: Sat, 14 Jul 2001 15:10:28 -0500
Subject: Ask-A-Microscopist: images of white and red blood cells

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Below is the result of your feedback form. It was submitted by
(tcote-at-medicacorp.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
13, 2001 at 12:35:36
---------------------------------------------------------------------------

Email: tcote-at-medicacorp.com
Name: Tyler Cote

Organization: Medica Corporation

Education: Undergraduate College

Location: Bedford, MA 01730

Question: Is is it possible to get clear images of white and red
blood cells using an air objective ?

I am curently working on a hematalogical assistant that uses a
digital camera to image white and red cells. The red cells seem to
be acting like little lenses. Most red cells appear to have a small
dark band around them. Upon further investigation it appears this
band is actually a series of colored bands.

When I wet the slide with a liquid that has an index of refraction
closer to that of the cells, the bands disapear. I am still using an
air objective, just looking at a wetted slide.

Is this common ?

Is there a way to get around having to wet the slides ?

Any help you can give is greatly appreciated.
Thank you.

Tyler Cote
Mechanical Engineer
Medica Corporation
Bedford, MA, 01370.

---------------------------------------------------------------------------


From daemon Sat Jul 14 15:17:12 2001



From: joelgerardo-at-correoweb.com ()
Date: Sat, 14 Jul 2001 15:09:58 -0500
Subject: Ask-A-Microscopist: Quality Control in the manufacturation of

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Below is the result of your feedback form. It was submitted by
(joelgerardo-at-correoweb.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
July 11, 2001 at 16:22:19
---------------------------------------------------------------------------

Email: joelgerardo-at-correoweb.com
Name: joel gerardo diaz M.D.

Organization: medicine faculty unam

Education: Graduate College

Location: mexico city , fedeeral district City, State, Country

Question: I need a manual of Quality Control in the manufacturation
of Colposcopes and Microscopes ,I will be very grateful for your
attention, sincerely. Joel gerardo Diaz
Sanchez M.D.

---------------------------------------------------------------------------


From daemon Mon Jul 16 02:10:37 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Mon, 16 Jul 2001 08:51:05 +0200
Subject: biofilm inclusion - any ideas

Contents Retrieved from Microscopy Listserver Archives
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Many thanks to those who responded to my request for information
about something that was found here in a biofilm preparation.

Consensus (almost 100%) is that it is a testate amoeba.




Rob Cross
Director : EM Unit, Rhodes University


From daemon Mon Jul 16 03:49:59 2001



From: NPGSlithography-at-aol.com
Date: Mon, 16 Jul 2001 10:23:21 EDT
Subject: Re: magnetic field shielding (2)

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First, I must precise again that I am not a specialist of magnetisme ! I
am only confronted with the same problem of electromagnetic interference.
So I asked this collegue which has answered and calculated only with the
hands.

What he said, and others have said it too, is than mu-metal is the best,
soft iron could work too. About Al or Cu, it's of course only efficient
with AC field. The goal is to disipate the AC electromagnetic filed in a
good conductor by Foucauld current. 50 or 150 Htz is not DC, even it is
not "high frequency". As a test is sometimes as good as much
calculations, we have tested with a 10 mm thick Al plate, 500 x 500 mm
large, aproching the SEM, and we have seen the distorsion reducing, but
not been supressed. I don't know if an Al box would be sufficient to put
down enought the interfernce. As we can move the SEM in an other room, we
didn't try further. The advice of my collegue was : "If you can't supress
the source, try to move the SEM ; shielding is often risky and expensive".
That what other have soon said on the list.

So about your suppositions (mail from F. Schamber):

} } Presumably your specialist has done the calculations and feels that eddy
} } current shielding will do the job. This suggests one of three things to
} } me: (1) he knows the problems are due to relatively high frequencies;
} } (2) your fields are very severe and he is only trying to knock them down
} } to a more tractable level; or (3) he knows something I don't. I would
} } personally be interested in learning more about how he arrived at this
} } solution. Specifically, what level of attenuation does he anticipate
} } achieving for 50 Hz magnetic fields with this kind of shielding?

it is the second witch is right ! We had 20-25 mGauss, 50-150 htz at the
sample level . That is much more that the 3 mG max recommended.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

---------- Forwarded message ----------


In a message dated 7/11/2001 3:14:47 PM Mountain Daylight Time,
micro-at-ldeo.columbia.edu writes:

} Can anyone recommend a good/cost-effective type of wall shielding to
} eliminate 60 and 180 cycle interferences coming into an SEM room from
} recently-installed large motors on the opposite side of the wall (that
} can't be moved now)?

In my previous reply, I forgot to mention that a listing of suppliers for
vibration and magnetic field canceling systems as well as suppliers for
mu-metal enclosures can be found at "www.jcnabity.com/links.htm".

(If anyone knows of other companies that should be listed, please let me
know.)

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Mon Jul 16 11:14:42 2001



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Mon, 16 Jul 2001 09:08:09 -0700
Subject: DTSA

Contents Retrieved from Microscopy Listserver Archives
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Ian;
I had the same import problem with Noran files. It seemed that the
Noran interpretation of the EMSA file spec is different than the
interpretation by the authors of DTSA.
--John Mardinly

-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-hrem.mpi-stuttgart.mpg.de]
Sent: Friday, July 13, 2001 4:54 AM
To: Microscopy Discussion Group


Thanks to all who replied to my question about the DTSA software package. I
have downloaded it onto a Mac here. As yet, I don't have it fully working
as it doesn't want to import the EMSA files exported by our Noran Voyager
system. I'm still trying to working out what the problem is, but in
principle it seems to be working (except with my files).

As helpfully pointed out by Scott Wight at NIST, the latest version resides
on their server at
http://www.cstl.nist.gov/div837/Division/outputs/DTSA/DTSA.htm

Thanks again

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/





From daemon Mon Jul 16 13:21:13 2001



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Mon, 16 Jul 2001 13:15:34 -0500
Subject: xl_30 ESEM and new EDS

Contents Retrieved from Microscopy Listserver Archives
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I am currently evaluating EDS packages for my Philips XL-30 ESEM with
LaB6 and wanted to poll the list members for their opinions, be they
positive or negative. How do members deal with the beam spread
issues? Can we trust the results? What about water vapor and ice
build up? As a multi-user facility how easy is it to get the data out
to the researcher these days? What about service quality?

I will post a summary so if you prefer your responses excluded,
please let me know.

Thanks

Scott Whittaker
SEM Lab Manager
National Museum of Natural History
Smithsonian Institution
10th & Constitution Ave, NW
Washington DC 20560-0104
202-357-1651


From daemon Mon Jul 16 13:21:13 2001



From: Fritz, Greg :      gfritz-at-thermonoran.com
Date: Mon, 16 Jul 2001 13:17:02 -0500
Subject: RE: DTSA

Contents Retrieved from Microscopy Listserver Archives
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The NORAN EMSA files follow the specifications fully and support all
varieties of EMSA options available. The problem is that DTSA only reads
one type of EMSA file format (X,Y pairs of data) which is not the default
condition of the VOYAGER files. The default format for VOYAGER stores only
the channel data in 5 columns. The default file format for VANTAGE was
changed to be X,Y pairs which is consistant with DTSA.

There is a solution for VOYAGER. You can save the EMSA format compatible
with DTSA by using the shell command:
emsa_io -x=1 -w={path to store to} -s={spectrum name to store}
example: emsa_io -x=1 -w=$HOME/spectra/myspec.emsa -s=spectrum1

When the next version of VOYAGER software is released the default condition
for the emsa_io program will be converted to the same as VANTAGE which will
make VOYAGER's default version of EMSA file format consistant with DTSA.

Greg Fritz

} -----Original Message-----
} } From: Mardinly, John [mailto:john.mardinly-at-intel.com]
} Sent: Monday, July 16, 2001 11:08 AM
} To: 'Ian MacLaren'; Microscopy Discussion Group
} Subject: RE: DTSA
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Ian;
} I had the same import problem with Noran files. It seemed that the
} Noran interpretation of the EMSA file spec is different than the
} interpretation by the authors of DTSA.
} --John Mardinly
}
} -----Original Message-----
} } From: Ian MacLaren [mailto:maclaren-at-hrem.mpi-stuttgart.mpg.de]
} Sent: Friday, July 13, 2001 4:54 AM
} To: Microscopy Discussion Group
} Subject: DTSA
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Thanks to all who replied to my question about the DTSA software package.
I
} have downloaded it onto a Mac here. As yet, I don't have it fully working
} as it doesn't want to import the EMSA files exported by our Noran Voyager
} system. I'm still trying to working out what the problem is, but in
} principle it seems to be working (except with my files).
}
} As helpfully pointed out by Scott Wight at NIST, the latest version
resides
} on their server at
} http://www.cstl.nist.gov/div837/Division/outputs/DTSA/DTSA.htm
}
} Thanks again
}
} Ian MacLaren
} Max Planck Institut f¸r Metallforschung, Seestraþe 92,
} 70174 Stuttgart, Germany
} Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
} Home page: http://members.tripod.co.uk/IanMacLaren/
}
}
}


From daemon Mon Jul 16 15:35:48 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 16 Jul 2001 16:28:16 -0400
Subject: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am not sure if anyone mentioned this, but there is a difference in using DTSA and the EMSA file handler plug-ins versions 1 and 2. Two did not work for me at all in any of the EMSA formatted files I tried, but version 1 did. I was using the power version of DTSA.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Monday, July 16, 2001 12:08 PM
To: 'Ian MacLaren'; Microscopy Discussion Group


Ian;
I had the same import problem with Noran files. It seemed that the
Noran interpretation of the EMSA file spec is different than the
interpretation by the authors of DTSA.
--John Mardinly

-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-hrem.mpi-stuttgart.mpg.de]
Sent: Friday, July 13, 2001 4:54 AM
To: Microscopy Discussion Group


Thanks to all who replied to my question about the DTSA software package. I
have downloaded it onto a Mac here. As yet, I don't have it fully working
as it doesn't want to import the EMSA files exported by our Noran Voyager
system. I'm still trying to working out what the problem is, but in
principle it seems to be working (except with my files).

As helpfully pointed out by Scott Wight at NIST, the latest version resides
on their server at
http://www.cstl.nist.gov/div837/Division/outputs/DTSA/DTSA.htm

Thanks again

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/





From daemon Mon Jul 16 15:35:48 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 16 Jul 2001 16:28:16 -0400
Subject: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am not sure if anyone mentioned this, but there is a difference in using DTSA and the EMSA file handler plug-ins versions 1 and 2. Two did not work for me at all in any of the EMSA formatted files I tried, but version 1 did. I was using the power version of DTSA.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Monday, July 16, 2001 12:08 PM
To: 'Ian MacLaren'; Microscopy Discussion Group


Ian;
I had the same import problem with Noran files. It seemed that the
Noran interpretation of the EMSA file spec is different than the
interpretation by the authors of DTSA.
--John Mardinly

-----Original Message-----
} From: Ian MacLaren [mailto:maclaren-at-hrem.mpi-stuttgart.mpg.de]
Sent: Friday, July 13, 2001 4:54 AM
To: Microscopy Discussion Group


Thanks to all who replied to my question about the DTSA software package. I
have downloaded it onto a Mac here. As yet, I don't have it fully working
as it doesn't want to import the EMSA files exported by our Noran Voyager
system. I'm still trying to working out what the problem is, but in
principle it seems to be working (except with my files).

As helpfully pointed out by Scott Wight at NIST, the latest version resides
on their server at
http://www.cstl.nist.gov/div837/Division/outputs/DTSA/DTSA.htm

Thanks again

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/





From daemon Mon Jul 16 16:33:10 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Mon, 16 Jul 2001 16:29:56 -0500
Subject: Fwd: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
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} To All following this lead,
Is anyone concerned about the probability that the treatment of women
described by several of the listers is illegal? Treating a woman like a
criminal or a delinquent child who needs to be closely monitored as to her
"motherhood" status is not only ridiculous, but descriminatory and
hopefully illegal. It is the responsibility of each individual in a
laboratory to watch out for his or her own safety and therefore the
business and responsibility of each woman in the lab to watch out for the
safety of her unborn child. We are all well past the kindergarten stage and
should no longer require a hand to hold while crossing the street.
Incensed in Iowa,
Kristen


} to all out there:
}
} This is what I have done so far:
} 1. Removed all stored chemicals from the lab.
} 2. Moved all chemical mixing into fume hood.
} 3. Met for 3 hours with environmental safety officer.
} 4. Met for 2 hours with radiation safety officer.
} 5. Eliminated all protocols which involve teratogens from pregnant
} workers routine.
} 6. Established a personal hygiene plan for pregnant worker, which is
} being signed of by worker, myself, director of the lab, safety
} officer, and her physician.
} 7. Reiterated safety protocols for worker, coat , gloves, safety
} glasses, etc.
} 8. Had lab monitored for radiation, passed.
} 9. Had lab monitored for formaldehyde gas, passed.
} 10. Planning to monitor xylene exposure to worker when thin sectioning
} (cannot do this in the hood, obviously). Heat pen( modified soldering
} iron) has not worked well so far, any ideas how to eliminate xylene?
} 11. Still looking for a non toxic general 1 micron stain for Spurrs and
} LR White. Any ideas?
} I am experimenting with food related stains (grape juice, etc.).
} 12. Employee has completed, lab safety training course, formaldehyde
} training course and radiation training course.
} 13. Will continue to monitor lab for chemical and radation exposure.
}
} To some this may seem like over kill, but I believe that it is very
} important that every stone is turned in regards to safety. Another
} issue that I face is that I have numerous grad students coming through
} the lab, and there is always a possibility that they are pregnant and do
} not realize it or keep it from me. I also have a nursing mother who was
} visiting the lab and never bothered to tell me, until the subject of why
} the lab was reorganized came up. I now demand that all female student,
} techs, visitors, ect.
} tell me of their "motherhood" status.
}
} Bill
}
} -----Original Message-----
} } From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
} Sent: Tuesday, July 10, 2001 7:28 AM
} To: William McManus; Monson, Frederick C.; Sally Stowe
} Cc: Microscopy Listserver
} Subject: RE: Tol blue/borax
}
}
} At 1:04 PM -0600 7/9/01, William McManus wrote:
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Mon Jul 16 17:42:48 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 16 Jul 2001 17:20:08 -0500
Subject: Core Facility Job

Contents Retrieved from Microscopy Listserver Archives
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The Associate Director position at the University of Missouri
Molecular Cytology Core (LM imaging facility) remains open.

This position involves training clients, developing new
methodologies, maintaining instrumentation and managing junior staff
members. Although many applicants will have a PhD in life sciences,
we would be happy to consider individuals without a PhD but who have
extensive experience in the relevant techniques. Opportunities for
individuals with PhD's to conduct independent or collaborative
research can be negotiated but the position is not tenure-track. The
salary range for this position is $30,257 -$54,663.00 and is 100%
benefit eligible. Opportunities for professional development will be
encouraged.

Our facility includes a BioRad Radiance 2000 Confocal Laser Scanning
Microscope system equipped with a Krypton/Argon laser (488 nm & 568
nm excitation lines) and Red laser diode (638 nm) laser mounted for
highest sensitivity in the Keller configuration (bottom port) of an
IX 70 Olympus inverted microscope. Simultaneous acquisition of three
different fluorescent labels, as well as a high quality Nomarski
image, is possible. Appropriate objectives, including water
immersion and long working distance objectives, are available. An
adjacent PC is equipped with the popular image analysis software
package MetaMorph (Universal Imaging). For wide-field fluorescence
work, we have another Olympus IX-70 inverted microscope equipped
with a Photometrics Sensys cooled CCD camera, a Ludl filter wheel on
the excitation side and a Ludl z axis stage motor, and a host of
Chroma HQ single, double and triple fluorochrome filters. The
Windows-based PC that controls this system includes the Vaytek
MicroTome deconvolution software package that does Nearest Neighbor,
No Neighbor, Constrained Iterative and Measure PSF deconvolution
algorithms. It runs under Image Pro Plus (Media Cybernetics) for the
image acquisition and 2D image analysis. For 3D work, we use
MetaMorph. This system is also equipped with a CRI color filter to
allow the acquisition of high quality color bright field images.
Objectives and condenser apertures for high resolution phase
contrast and differential interference contrast (DIC, Nomarski) are
available. We also have a Nikon Optiphot upright photomicroscope
with bright field, phase, DIC and fluorescence optics. This system
is coupled to a Spot2 chilled CCD digital camera and a second copy
of the MetaMorph image analysis and processing software. The main
laboratory of the MCC includes a Reichert Frigocut 2800 cryostat for
generating 2-100 µm thick frozen sections and a Reichert UltraCut S
ultramicrotome for preparation of semi-thin (0.5 -1 µm) LM sections
and ultra-thin EM sections. The MCC also contains two Apple
workstations with extensive software for image manipulation and
presentation graphics. For publication quality output, the Core is
equipped with a Polaroid ProPallette 7000 film recorder and a Fuji
Pictrography 3000 for photographic quality color prints. In the
last year, our facility attracted researchers from the laboratories
of over 100 different principal investigators located in 26 different
departments or units on our campus.

Although an ideal candidate would have experience in confocal
scanning laser microscopy, bright field microscopy, wide field
fluorescence microscopy, deconvolution, image
processing/analysis, all types of microtomy, immunocytochemistry, in
situ hybridization and Adobe Photoshop, candidates with extensive
experience in selected areas and who have the desire and capacity to
learn the additional areas will be considered. Excellent oral and
written communication skills are essential. Experience in a
multi-user core facility would be viewed positively. Electron
microscopy is not a component of this core facility. Women and
minority candidates are especially encouraged to apply. Review of
applications will begin immediately and continue until an appropriate
candidate is hired. This position has a salary range of $30,257
$54,663.00 and is 100% benefit eligible. Opportunities for
professional development will be encouraged. Columbia is a great,
inexpensive place to live.

The Core's web site can be viewed at
http://www.biotech.missouri.edu/mbp/cores/index.html

Address applications (CV and 3 letters of reference) or inquires to:

Thomas E. Phillips, Ph.D.
Division of Biological Sciences
3 Tucker Hall, University of Missouri
Columbia, MO 65211-7400.
573-882-4712
PhillipsT-at-missouri.edu




--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Mon Jul 16 17:42:50 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 16 Jul 2001 18:38:12 -0400
Subject: Fwd: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree in principal. But, we live in a very litigious, cover-your-ass type of world. It behooves employers and supervisors to document the training and warnings that women (and men) receive with respect to chemical hygiene in the laboratory environment -especially with respect to pregnancy. I would wager to bet that even the most idealist feminist would be looking for litigation relief if they were exposed to hazardous materials during their pregnancy even if it was her own fault with respect to educating herself to the dangers.

I think that people should be warned of the dangers and that warning should be documented. Their impending motherhood (or lack of) should be their own business. If a woman is pregnant and the unborn child can be potentially exposed to hazardous chemicals, the employer can only make adjustments to the woman's work environment if they are told. If they are not, they should not be held accountable. To protect the employer, there should also be documentation that determines when a woman first tells the company. Even when the training and documentation is there and the employee chooses to ignore them, a company can still be held responsible.

And another thing: this thread is about responsible scientists doing things to protect beings that can not protect themselves. They approached this problem in a responsible, caring and concerned way, not to subjugate women. It is nothing to get incensed about.

CYA rules.

These are just my humble opinions.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Kristen Lennon [mailto:kalen-at-iastate.edu]
Sent: Monday, July 16, 2001 5:30 PM
To: Microscopy-at-sparc5.microscopy.com



} To All following this lead,
Is anyone concerned about the probability that the treatment of women
described by several of the listers is illegal? Treating a woman like a
criminal or a delinquent child who needs to be closely monitored as to her
"motherhood" status is not only ridiculous, but descriminatory and
hopefully illegal. It is the responsibility of each individual in a
laboratory to watch out for his or her own safety and therefore the
business and responsibility of each woman in the lab to watch out for the
safety of her unborn child. We are all well past the kindergarten stage and
should no longer require a hand to hold while crossing the street.
Incensed in Iowa,
Kristen


} to all out there:
}
} This is what I have done so far:
} 1. Removed all stored chemicals from the lab.
} 2. Moved all chemical mixing into fume hood.
} 3. Met for 3 hours with environmental safety officer.
} 4. Met for 2 hours with radiation safety officer.
} 5. Eliminated all protocols which involve teratogens from pregnant
} workers routine.
} 6. Established a personal hygiene plan for pregnant worker, which is
} being signed of by worker, myself, director of the lab, safety
} officer, and her physician.
} 7. Reiterated safety protocols for worker, coat , gloves, safety
} glasses, etc.
} 8. Had lab monitored for radiation, passed.
} 9. Had lab monitored for formaldehyde gas, passed.
} 10. Planning to monitor xylene exposure to worker when thin sectioning
} (cannot do this in the hood, obviously). Heat pen( modified soldering
} iron) has not worked well so far, any ideas how to eliminate xylene?
} 11. Still looking for a non toxic general 1 micron stain for Spurrs and
} LR White. Any ideas?
} I am experimenting with food related stains (grape juice, etc.).
} 12. Employee has completed, lab safety training course, formaldehyde
} training course and radiation training course.
} 13. Will continue to monitor lab for chemical and radation exposure.
}
} To some this may seem like over kill, but I believe that it is very
} important that every stone is turned in regards to safety. Another
} issue that I face is that I have numerous grad students coming through
} the lab, and there is always a possibility that they are pregnant and do
} not realize it or keep it from me. I also have a nursing mother who was
} visiting the lab and never bothered to tell me, until the subject of why
} the lab was reorganized came up. I now demand that all female student,
} techs, visitors, ect.
} tell me of their "motherhood" status.
}
} Bill
}
} -----Original Message-----
} } From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
} Sent: Tuesday, July 10, 2001 7:28 AM
} To: William McManus; Monson, Frederick C.; Sally Stowe
} Cc: Microscopy Listserver
} Subject: RE: Tol blue/borax
}
}
} At 1:04 PM -0600 7/9/01, William McManus wrote:
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Mon Jul 16 21:08:52 2001



From: Bradley, Steven :      sabradle-at-uop.com
Date: Mon, 16 Jul 2001 13:37:14 -0500
Subject: Confocal Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone recommend a vendor for a confocal microscope for materials
science use? We would like to discuss a materials science application with
a knowledgeable vendor..

Thanks,
Steve Bradley
UOP
50 E Algonquin Road
Des Plaines, IL 60017
1-847-391-3321

sabradle-at-uop.com




From daemon Mon Jul 16 22:01:12 2001



From: Dave Roberts :      dave-at-boeckeler.com
Date: Mon, 16 Jul 2001 21:57:46 -0500
Subject: 7th Annual Materials Microtomy Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This year we will be hosting the 7th Annual RMC Materials Microtomy Short
Course in Tucson, Arizona from October 23 - 26.

For those of you residing in colder climes Tucson has just the weather to
chase away the onset of those winter-time blues.

Join us, and our internationally renowned course faculty, in sunny Tucson to
participate in this unique event. This short course is designed specifically
for researchers in the field of materials science who wish to gain exposure
to advances in specimen preparation for electron microscopy.

email stacy-at-boeckeler.com to receive full details and a course brochure

Dave Roberts
Director - RMC EM Products
Boeckeler Instruments, Inc
4650 South Butterfield Drive
Tucson, AZ 85714
Tel: (520) 745-0001 Fax: (520) 745-0004
email: dave-at-boeckelr.com
website: www.rmcproducts.com


From daemon Tue Jul 17 01:21:23 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Tue, 17 Jul 2001 02:20:48 -0400
Subject: Child care solutions for M & M 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists:

I've registered for M & M 2001 in Long Beach, but I'm temporarily a single
parent during the event, and need to make arrangements for my six-year-old
child. One option is to bring her with me...but I need to make sure she is
looked after during the short courses and some of the scientific symposia.
If some local listers could give me any suggestions for care, or if there's
anyone else in the same position who would be willing to pool expenses, I
would be very grateful if you could contact me (you can e-mail me directly).

Thanks in advance,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Tue Jul 17 03:01:56 2001



From: Emond W F de Roever :      ederoever-at-ONDEO-Nalco.com
Date: Tue, 17 Jul 2001 09:48:00 +0200
Subject: Re: xl_30 ESEM and new EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Scott,

Responding to your question:

"I am currently evaluating EDS packages for my Philips XL-30 ESEM with
LaB6 .................What about water vapor and ice
build up? ....

I acquired a (Si) detector from Oxford for our ESEM 2020 in 1995. At that
moment half a dozen detectors had been sold by Oxford to ESEM users, half a
dozen by Noran, and one by EDAX. Oxford offered in its software package the
possibility of "conditioning" the detector, a partial warm-up to remove ice
etc., which takes two hours only. The other companies did not offer that
possibility at that time, probably because it was patented. I do not know
whether they offer it by now. It is very easy. I have used it for 7 years
now, without problems so far. I believe that regular "conditioning" in
combination with other measures has allowed this. Other measures: 1. a
protective tube in which the detector is retracted when not operating (or
during hot-stage operation, to protect from hot dust); and 2. maintaining a
low pressure in the sample chamber (1 Torr instead of the regular 5 T),
when the ESEM is not used.

As to : " How do members deal with the beam spread issues? " ,

X-ray mapping is a good method to eliminate the effects of beam spreading,
and check particle analyses. Furthermore, beam spreading can be reduced by
working at the lowest pressure possible without getting charging (I use
0.9-1 Torr routinely) and by using the detector set-up (the elongated
detector/bullit in the ESEM 2020) with the shortest path in the
higher-presssure surrounding, i.e. the sample chamber. I know that for
quantitative analysis new software programmes exist which allow e.g. to
extrapolate the results from a higher-pressure analysis + a lower-pressure
analysis to zero pressure, or use some method of background/surroundings
subtraction (several methods have been mentioned in the literature), but my
(older) software does not offer that possibility. With best regards, Emond
de Roever

Ondeo-Nalco Europe, Oegstgeest, the Netherlands





From daemon Tue Jul 17 05:49:01 2001



From: Dr. Catherine Powell :      POWCA-at-reg2.health.nb.ca
Date: Tue, 17 Jul 2001 07:40:00 -0300
Subject: Survey-EM Workload

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are a hospital EM Unit performing surgical pathology examinations and virology exams on clinical specimens. We perform EM on about 130 surgical cases per year (works out to about 150 specimens, nearly half of which are kidneys, the remainder are solid tissue tumors, muscle, nerve). We perform direct EM on about 300 stools/vesicle fluids for virus examination per year.

We have 1.4 FTEs (full-time equivalents). Our .4FTE covers leaves and off-sets workload. We are a manual service.

Our TAT for pathology is 3 working days from processing (day 1), thick sectioning/consult with pathologist/thin sectioning, staining/scoping (by technologist-specialist)/photography (day 2), photographic printing and reporting to pathologist (day 3).

Our TAT for virology is same-day to 72 hours.

How do others compare?

Thank you in advance for your input.

C. Powell
powca-at-reg2.health.nb.ca



From daemon Tue Jul 17 07:39:09 2001



From: Dr. Catherine Powell :      POWCA-at-reg2.health.nb.ca
Date: Tue, 17 Jul 2001 09:30:55 -0300
Subject: Biopsy EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone ever worked out an estimate of how many "man staff-hours" are involved in processing a biopsy specimen, manually (pardon me again!), for EM? This includes scoping, interpretation and reporting.

Thank you,

C. Powell
EM Unit
Saint John Regional Hospital
NB, Canada
powca-at-reg2.health.nb.ca





From daemon Tue Jul 17 08:19:56 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Tue, 17 Jul 2001 10:13:57 -0300
Subject: HMDS recipe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been critical point drying scallop specimens successfully for SEM to
examine them as they grow. We are now at a point where the specimens no
longer fit in the CPD chamber. With only a couple of specimens to go we
thought to try HMDS. The samples are approximately 30mm in diameter. Does
someone have a protocol they have used for larger specimens that has worked
for them? You can send them directly to me and I can summarize for the
list. Thanks in advance.


David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford St.
Halifax, NS B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca
http://www.imb.nrc.ca/micros_e.html


From daemon Tue Jul 17 08:29:08 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Tue, 17 Jul 2001 09:26:09 -0400
Subject: e mail address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I have lost the e-mail address of Lesley Bechtold of Jackson Labs. Can some
one help me out? Thanks, Tim

Timothy Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 238 JAH
1020 Locust Street
19107
215-513-4798 Work
610-613-8170 Cell



From daemon Tue Jul 17 10:08:50 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Tue, 17 Jul 2001 11:05:02 -0400
Subject: RE: xl_30 ESEM and new EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Scott and listers,

I can add a few points to this reply. Since you have the XL30, you will
generally be leaving it in Hivac overnight and when not in use for ESEM. Ice
buildup should be a lesser problem for you; depending of course on the nature
of your work. I have the Oxford system on our XL30 ESEM-FEG and have had good
results with the package and with the service (rarely needed). I did not
purchase the package myself so did not evaluate competitors' offerings. I
don't know which detectors you have but the lower-pressure ones are best for
EDS: Large Field Detector or CP+ insert with Backscattered ED will operate at
much less than 1T. If you need higher pressures; use the extended cone X-ray
detectors. I have used the conditioner on occassion; I believe this has been a
feature on Link systems since the early 90's at least. Monitor low-end
sensitivity with NiLa vs. NiKa.

I find that beam skirt is a minor issue; generally the ESEM samples (customers)
are not using EDS. When you need both, spot-check surrounding areas, or run a
few maps at low resolution, and be less "quantitative" about your conclusions
unless you need to get into the corrections mentioned by Emond.

The new Oxford system is significantly different from mine, so I won't talk
about ease-of-use and portability of data.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Tuesday, July 17, 2001 3:48 AM, Emond W F de Roever
[SMTP:ederoever-at-ONDEO-Nalco.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Scott,
}
} Responding to your question:
}
} "I am currently evaluating EDS packages for my Philips XL-30 ESEM with
} LaB6 .................What about water vapor and ice
} build up? ....
}
} I acquired a (Si) detector from Oxford for our ESEM 2020 in 1995. At that
} moment half a dozen detectors had been sold by Oxford to ESEM users, half a
} dozen by Noran, and one by EDAX. Oxford offered in its software package the
} possibility of "conditioning" the detector, a partial warm-up to remove ice
} etc., which takes two hours only. The other companies did not offer that
} possibility at that time, probably because it was patented. I do not know
} whether they offer it by now. It is very easy. I have used it for 7 years
} now, without problems so far. I believe that regular "conditioning" in
} combination with other measures has allowed this. Other measures: 1. a
} protective tube in which the detector is retracted when not operating (or
} during hot-stage operation, to protect from hot dust); and 2. maintaining a
} low pressure in the sample chamber (1 Torr instead of the regular 5 T),
} when the ESEM is not used.
}
} As to : " How do members deal with the beam spread issues? " ,
}
} X-ray mapping is a good method to eliminate the effects of beam spreading,
} and check particle analyses. Furthermore, beam spreading can be reduced by
} working at the lowest pressure possible without getting charging (I use
} 0.9-1 Torr routinely) and by using the detector set-up (the elongated
} detector/bullit in the ESEM 2020) with the shortest path in the
} higher-presssure surrounding, i.e. the sample chamber. I know that for
} quantitative analysis new software programmes exist which allow e.g. to
} extrapolate the results from a higher-pressure analysis + a lower-pressure
} analysis to zero pressure, or use some method of background/surroundings
} subtraction (several methods have been mentioned in the literature), but my
} (older) software does not offer that possibility. With best regards, Emond
} de Roever
}
} Ondeo-Nalco Europe, Oegstgeest, the Netherlands
}
}



From daemon Tue Jul 17 11:19:16 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 17 Jul 2001 11:12:04 -0500
Subject: RE: xl_30 ESEM and new EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On my three year old XL30 I usually work at 6 Torr
(to keep specimens really wet) and working distance 7.5 mm.
It is routine work to get decent maps or line scans of Ca, P,
S (4-10% consentration). If you do not need trace elements or
quantitative analysis, EDS on ESEM will do pretty good work.

If you samples are not wet, just not conductive, then you
can go to higher voltage, lower pressure (1 Torr or less)
and use BSE, which is less influenced by charging and then
it will be difficalt to find difference with conventional
high vacuum EDS analysis.

My detector have never had ice build up, but oil contamination
became substantial in a few weeks after cleaning. As far as I
know others do not have this particular problem, and I am loosing
my battle with EDS and microscope manufacturers.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
} Sent: Monday, July 16, 2001 1:16 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: xl_30 ESEM and new EDS
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} I am currently evaluating EDS packages for my Philips XL-30 ESEM with
} LaB6 and wanted to poll the list members for their opinions, be they
} positive or negative. How do members deal with the beam spread
} issues? Can we trust the results? What about water vapor and ice
} build up? As a multi-user facility how easy is it to get the data out
} to the researcher these days? What about service quality?
}
} I will post a summary so if you prefer your responses excluded,
} please let me know.
}
} Thanks
}
} Scott Whittaker
} SEM Lab Manager
} National Museum of Natural History
} Smithsonian Institution
} 10th & Constitution Ave, NW
} Washington DC 20560-0104
} 202-357-1651
}
}
}


From daemon Tue Jul 17 11:21:40 2001



From: Edwards Forensic Service :      fedwards-at-mninter.net
Date: Tue, 17 Jul 2001 11:20:37 -0600
Subject: Re: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I seen several comments on this problem. It is a legal minefield. It
is important for companies to get an expert attorney's advice on this
matter. Several years ago a car battery manufacturer tried to limit the
exposure of pregnant women to lead by re-assigning them to different jobs.
The case went to the U.S. Supreme Court and the company lost. The Court
said in an unanimous opinion that the company must make the workplace safe
for all employees equally.
A better approach is to give all employees, regardless of gender, the
same safety education at the time of hiring and to periodically update this
as needed. In other words men would also be told of the hazards for
pregnant individuals.
The best approach is to simply make the workplace safe for pregnant
women.
Get proper legal advice. This is not for amateurs.

Harold Edwards, Ph.D.
Minneapolis, MN.



From daemon Tue Jul 17 11:52:41 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Tue, 17 Jul 2001 09:47:37 -0700
Subject: Re: Child care solutions for M & M 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick:

I can ask around with some of our friends who watch our children to see if anyone would be
interested in heading up to Long Beach. We are about a 1 hour drive from the convention
center so I would guess they would want to do a whole day thing rather than just a few
hours. If you think that would help, let me know and I will see what I can arrange.

Best regards-

David

Rick Powell at Nanoprobes wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Microscopists:
}
} I've registered for M & M 2001 in Long Beach, but I'm temporarily a single
} parent during the event, and need to make arrangements for my six-year-old
} child. One option is to bring her with me...but I need to make sure she is
} looked after during the short courses and some of the scientific symposia.
} If some local listers could give me any suggestions for care, or if there's
} anyone else in the same position who would be willing to pool expenses, I
} would be very grateful if you could contact me (you can e-mail me directly).
}
} Thanks in advance,
}
} Rick Powell
}
} *****************************************************************************************
} Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
} NANOPROBES, Incorporated
} 95 Horse Block Road, Yaphank, NY 11980-9710, USA
}
} US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
} 980-3608
}
} Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
} *****************************************************************************************

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Tue Jul 17 11:56:27 2001



From: Christopher M May :      cmmay-at-US.ibm.com
Date: Tue, 17 Jul 2001 12:51:50 -0400
Subject: Titanium Particle dispersion for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am working with a TEM and I would like to know the best way to disperse
Titanium Oxide particles for analysis.
I am currently using a methanol solution, and it is not working very well.


Sincerely,

Christopher May
IBM Analytical Services



From daemon Tue Jul 17 12:02:02 2001



From: Jan Markham :      afp042-at-email.sps.mot.com
Date: Tue, 17 Jul 2001 09:57:31 -0700
Subject: Re: Fwd: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry Kristen,

Even though I am a female, I have to agree with Scott on this one.
People will seize any opportunity to sue, no matter how great their
understanding of the risks involved ahead of time - I have seen it
myself in a lab situation. I would love to think that people would take
responsibility for their own actions, but that just isn't the reality
these days - the lure of easy money always ends up being too great. So,
as Scott says, you CYA as best you can and keep your fingers crossed.

Jan Markham
Motorola

Kristen Lennon wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } To All following this lead,
} Is anyone concerned about the probability that the treatment of women
} described by several of the listers is illegal? Treating a woman like a
} criminal or a delinquent child who needs to be closely monitored as to her
} "motherhood" status is not only ridiculous, but descriminatory and
} hopefully illegal. It is the responsibility of each individual in a
} laboratory to watch out for his or her own safety and therefore the
} business and responsibility of each woman in the lab to watch out for the
} safety of her unborn child. We are all well past the kindergarten stage and
} should no longer require a hand to hold while crossing the street.
} Incensed in Iowa,
} Kristen
}
} } to all out there:
} }
} } This is what I have done so far:
} } 1. Removed all stored chemicals from the lab.
} } 2. Moved all chemical mixing into fume hood.
} } 3. Met for 3 hours with environmental safety officer.
} } 4. Met for 2 hours with radiation safety officer.
} } 5. Eliminated all protocols which involve teratogens from pregnant
} } workers routine.
} } 6. Established a personal hygiene plan for pregnant worker, which is
} } being signed of by worker, myself, director of the lab, safety
} } officer, and her physician.
} } 7. Reiterated safety protocols for worker, coat , gloves, safety
} } glasses, etc.
} } 8. Had lab monitored for radiation, passed.
} } 9. Had lab monitored for formaldehyde gas, passed.
} } 10. Planning to monitor xylene exposure to worker when thin sectioning
} } (cannot do this in the hood, obviously). Heat pen( modified soldering
} } iron) has not worked well so far, any ideas how to eliminate xylene?
} } 11. Still looking for a non toxic general 1 micron stain for Spurrs and
} } LR White. Any ideas?
} } I am experimenting with food related stains (grape juice, etc.).
} } 12. Employee has completed, lab safety training course, formaldehyde
} } training course and radiation training course.
} } 13. Will continue to monitor lab for chemical and radation exposure.
} }
} } To some this may seem like over kill, but I believe that it is very
} } important that every stone is turned in regards to safety. Another
} } issue that I face is that I have numerous grad students coming through
} } the lab, and there is always a possibility that they are pregnant and do
} } not realize it or keep it from me. I also have a nursing mother who was
} } visiting the lab and never bothered to tell me, until the subject of why
} } the lab was reorganized came up. I now demand that all female student,
} } techs, visitors, ect.
} } tell me of their "motherhood" status.
} }
} } Bill
} }
} } -----Original Message-----
} } } From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
} } Sent: Tuesday, July 10, 2001 7:28 AM
} } To: William McManus; Monson, Frederick C.; Sally Stowe
} } Cc: Microscopy Listserver
} } Subject: RE: Tol blue/borax
} }
} }
} } At 1:04 PM -0600 7/9/01, William McManus wrote:
} } } -----------------------------------------------------------------------
} } -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------
}
} Kristen A. Lennon, Ph.D.
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
} 515-294-8854
} kalen-at-iastate.edu
} www.baumlab.org


From daemon Tue Jul 17 13:58:45 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 17 Jul 2001 14:36:21 -0400
Subject: Child care solutions for M & M 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Other organizations have child care options. I believe that the AVS has had something available and the ICMCTF meeting has been talking about it. I have a colleague who used it at the AVS and I believe that she said it was on the expensive side, but that it did enable her to go to the meeting. I know of other colleagues who have not attended meetings because of conflicting schedules with their spouses.

Perhaps this is a topic that should be discussed at the MSA and MAS business meetings. Since my kids are grown, it doesn't affect me, but I can understand the problem.

Another option is to use a forum such as the listserver to organize a child care pool or to pool the resources of those members that need such a service.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Rick Powell at Nanoprobes [mailto:rpowell-at-nanoprobes.com]
Sent: Tuesday, July 17, 2001 2:21 AM
To: Microscopy-at-sparc5.microscopy.com


Hello Microscopists:

I've registered for M & M 2001 in Long Beach, but I'm temporarily a single
parent during the event, and need to make arrangements for my six-year-old
child. One option is to bring her with me...but I need to make sure she is
looked after during the short courses and some of the scientific symposia.
If some local listers could give me any suggestions for care, or if there's
anyone else in the same position who would be willing to pool expenses, I
would be very grateful if you could contact me (you can e-mail me directly).

Thanks in advance,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Tue Jul 17 14:35:27 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Monday, July 16, 2001 5:38 PM
Subject: Fwd: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Along this vane, We became concerned about safety issues quite a number of years ago. We try to train users of this facility as to the dangers involved and appropriate scientific procedures...regardless of sex, age, etc.
We have made it a point to provide sufficent hoods, etc so that users can work safely. We also make a point of the fact that it is the researcher's responsibility to inform themselves of hazards and to ask questions if they are not sure of appropriate procedure. Material safety data sheets are also available in an indexed binder so that anyone can easily check on specific reagents.

In addition we have them sign a sheet when they first start using the facility which says that they understand the potential risks (broad categories of possible hazards are listed on the sheet) and assume the responsibility for their own actions. This may not hold up completely in a court of law but it does show an attempt on our part to make the user aware of their own responsibility for their actions.

Safety is everyone's concern regardless of sex or biological conditions. Whatever policies are in place should be there for everyone, not just that occasional special situation. To have a lab manager or supervisor demand private medical information about users is most certainly an invasion of privacy.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907


--------------------------------------


I agree in principal. But, we live in a very litigious, cover-your-ass type of world. It behooves employers and supervisors to document the training and warnings that women (and men) receive with respect to chemical hygiene in the laboratory environment -especially with respect to pregnancy. I would wager to bet that even the most idealist feminist would be looking for litigation relief if they were exposed to hazardous materials during their pregnancy even if it was her own fault with respect to educating herself to the dangers.

I think that people should be warned of the dangers and that warning should be documented. Their impending motherhood (or lack of) should be their own business. If a woman is pregnant and the unborn child can be potentially exposed to hazardous chemicals, the employer can only make adjustments to the woman's work environment if they are told. If they are not, they should not be held accountable. To protect the employer, there should also be documentation that determines when a woman first tells the company. Even when the training and documentation is there and the employee chooses to ignore them, a company can still be held responsible.

And another thing: this thread is about responsible scientists doing things to protect beings that can not protect themselves. They approached this problem in a responsible, caring and concerned way, not to subjugate women. It is nothing to get incensed about.

CYA rules.

These are just my humble opinions.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Kristen Lennon [mailto:kalen-at-iastate.edu]
Sent: Monday, July 16, 2001 5:30 PM
To: Microscopy-at-sparc5.microscopy.com



} To All following this lead,
Is anyone concerned about the probability that the treatment of women
described by several of the listers is illegal? Treating a woman like a
criminal or a delinquent child who needs to be closely monitored as to her
"motherhood" status is not only ridiculous, but descriminatory and
hopefully illegal. It is the responsibility of each individual in a
laboratory to watch out for his or her own safety and therefore the
business and responsibility of each woman in the lab to watch out for the
safety of her unborn child. We are all well past the kindergarten stage and
should no longer require a hand to hold while crossing the street.
Incensed in Iowa,
Kristen


} to all out there:
}
} This is what I have done so far:
} 1. Removed all stored chemicals from the lab.
} 2. Moved all chemical mixing into fume hood.
} 3. Met for 3 hours with environmental safety officer.
} 4. Met for 2 hours with radiation safety officer.
} 5. Eliminated all protocols which involve teratogens from pregnant
} workers routine.
} 6. Established a personal hygiene plan for pregnant worker, which is
} being signed of by worker, myself, director of the lab, safety
} officer, and her physician.
} 7. Reiterated safety protocols for worker, coat , gloves, safety
} glasses, etc.
} 8. Had lab monitored for radiation, passed.
} 9. Had lab monitored for formaldehyde gas, passed.
} 10. Planning to monitor xylene exposure to worker when thin sectioning
} (cannot do this in the hood, obviously). Heat pen( modified soldering
} iron) has not worked well so far, any ideas how to eliminate xylene?
} 11. Still looking for a non toxic general 1 micron stain for Spurrs and
} LR White. Any ideas?
} I am experimenting with food related stains (grape juice, etc.).
} 12. Employee has completed, lab safety training course, formaldehyde
} training course and radiation training course.
} 13. Will continue to monitor lab for chemical and radation exposure.
}
} To some this may seem like over kill, but I believe that it is very
} important that every stone is turned in regards to safety. Another
} issue that I face is that I have numerous grad students coming through
} the lab, and there is always a possibility that they are pregnant and do
} not realize it or keep it from me. I also have a nursing mother who was
} visiting the lab and never bothered to tell me, until the subject of why
} the lab was reorganized came up. I now demand that all female student,
} techs, visitors, ect.
} tell me of their "motherhood" status.
}
} Bill
}
} -----Original Message-----
} } From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
} Sent: Tuesday, July 10, 2001 7:28 AM
} To: William McManus; Monson, Frederick C.; Sally Stowe
} Cc: Microscopy Listserver
} Subject: RE: Tol blue/borax
}
}
} At 1:04 PM -0600 7/9/01, William McManus wrote:
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
515-294-8854
kalen-at-iastate.edu
www.baumlab.org





From daemon Tue Jul 17 15:01:51 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 17 Jul 2001 15:55:47 -0400
Subject: Titanium Particle dispersion for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I assume that you have tried other solvents as well, including water. You might consider adding a small surfactant.

One method that has worked for me for diamond particles is to ultrasonic a suspension of water and let it set for a few moments and then pour some of the liquid into a nebulizer. I then use the nebulizer over the surface that I want to disperse the diamond particles on while it is on a hot plate at about 120C.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Christopher M May [mailto:cmmay-at-US.ibm.com]
Sent: Tuesday, July 17, 2001 12:52 PM
To: microscopy-at-sparc5.microscopy.com


Hello,

I am working with a TEM and I would like to know the best way to disperse
Titanium Oxide particles for analysis.
I am currently using a methanol solution, and it is not working very well.


Sincerely,

Christopher May
IBM Analytical Services



From daemon Tue Jul 17 18:08:55 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 17 Jul 2001 18:58:00 -0400 (EDT)
Subject: RE: Child care solutions for M & M 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sometimes hotels have child care services or nannies who will come to
rooms. Perhaps several folks could go in together and pool resources. Try
calling your hotel concierge.




Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Jul 17 18:40:21 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 18 Jul 2001 00:37:47 +0100
Subject: Re: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hear hear!

} Safety is everyone's concern regardless of sex or biological
conditions. Whatever policies are in place should be there for
everyone, not just that occasional special situation. To have a lab
manager or supervisor demand private medical information about users
is most certainly an invasion of privacy.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-099e Whistler Building
} West Lafayette, IN 47907




From daemon Tue Jul 17 18:44:16 2001



From: Meghan Towner :      town3648-at-uidaho.edu
Date: Tue, 17 Jul 2001 16:38:16 -0700 (PDT)
Subject: tissue preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello-
I am a grad student at the University of Idaho and would like some input on a protocol for fixing rat tissue in order to view their spinal ganglia on the TEM. First heparin is injected intracardiac, then perfusion via the heart with saline (37C) followed by paraformaldehyde (4%, 15C). The vertebral column is then removed and immersed in 4% paraformaldehyde for several hours before dissecting the spinal ganglia. This protocol was given to me by my advisor, but I have been unable to find a similar method cited in the literature. Also, I have used this method and found all the mitochondria have already lost their cristae. Any suggestions and comments would be appreciated.
Thank you.
--Meghan Towner


From daemon Tue Jul 17 20:40:18 2001



From: Damian Neuberger :      neuberger1234-at-home.com
Date: Tue, 17 Jul 2001 20:35:17 -0500
Subject: Re: Titanium Particle dispersion for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

Of course, your technique will be selecting for the smaller particles as the
larger ones as well as the agglomerates will be dropping out while it sets
before you decant into the nebulizer. Nebulizing will also have issues
about particle selection.

The original requester didn't say what the size range of the particles are
but I assume that they must be submicron or nanometer range as he is using
the TEM. Was he trying to size them, study their morphology, or what? I
usually end up trying a variety of suspending fluids and also use on
ocassion water with surfactant. But there are other techniques to use
depending on what we're trying to find out about the particles.

Damian Neuberger
Senior Research Scientist
Particle Science and Technology
Baxter Healthcare Corp

} I assume that you have tried other solvents as well, including water. You
might consider adding a small surfactant.
}
} One method that has worked for me for diamond particles is to ultrasonic a
suspension of water and let it set for a few moments and then pour some of
the liquid into a nebulizer. I then use the nebulizer over the surface that
I want to disperse the diamond particles on while it is on a hot plate at
about 120C.
}
} -Scott
}




From daemon Tue Jul 17 20:50:04 2001



From: cokendolpher-at-aol.com ()
Date: Tue, 17 Jul 2001 20:46:44 -0500
Subject: Ask-A-Microscopist: LM SL3D microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(cokendolpher-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
July 17, 2001 at 19:33:06
---------------------------------------------------------------------------

Email: cokendolpher-at-aol.com
Name: James Cokendolpher

Organization: Midwestern State University

Education: Graduate College

Location: Lubbock, Texas USA

Question: I have used an AO150 for my home research for the past 20
or so years. With the advent of good digital cameras I am now
thinking of buying an SL3D microscope and puting my camera lucida in
a museum. Do you know of any other microscope with a greater depth
of field for under $3000? Do you know of any articles that are good
or bad about the SL3D; other than those on their website? My work
involves the description and illustration of minute arthropod parts.
Thanks.

---------------------------------------------------------------------------


From daemon Tue Jul 17 21:03:12 2001



From: Rosalie Daniel :      rosalie-at-deakin.edu.au
Date: Wed, 18 Jul 2001 11:53:48 +1000
Subject: Removing Spurrs Resin from sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I have a question regarding the removal of Spurrs resin from sections on
slides. I want to use aniline blue as a callose stain for plant tissue. I
have sections (embedded in Spurrs) on slides. The protocol I have to
remove the resin uses sodium methoxide and benzene (Mayor, Hampton and
Rosario, 1961). I was wondering if anyone can suggest a protocol that is
less dangerous than using the sodium methoxide. Or, alternatively, has
anyone used this technique, or a similar one and had any problems/success
with it?
Thanks,
Rose


From daemon Wed Jul 18 02:35:39 2001



From: l.keddie-at-mluri.sari.ac.uk
Date: Wed, 18 Jul 2001 08:29:26 +0100
Subject: Electron Microscopist Vacancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{center} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} {/param} {sm=
aller} {smaller} {smaller} {smaller} {smaller} _ {FontFamily} {param} Book Antiqua=
{/param} {bigger} {bigger} {bigger} {bigger} {bigger} {/center}

{center} {bold} {bigger} {bigger} ANALYTICAL GROUP {/center}


{center} ELECTRON MICROSCOPIST {/center}


{flushboth} {/bold} {smaller} {smaller} Applications are invited from suitably=
qualified and experienced
candidates for the above post. The successful candidate will be
responsible for providing an electron microscopy service in
support of the Institute's research programme and on a contract
basis to commercial organisations. {/flushboth}


{flushboth} Candidates should possess a degree in an appropriate subject an=
d
be experienced in the analysis of a wide range of materials by
scanning electron microscopy. {/flushboth}


{flushboth} Starting salary, depending upon qualifications and experience,
will be normally within the range =A318,500 - =A321,400 per annum
(performance related to a maximum of =A3 26,000). Non-
contributory Superannuation Scheme. {/flushboth}


MLURI receives funding from the Scottish Executive Environment
and Rural Affairs Department.


{flushboth} Further particulars and application forms can be obtained from
Personnel, The Macaulay Land Use Research Institute,
Craigiebuckler, Aberdeen, AB15 8QH Tel 01224-498200, Fax 01224-
311556, E-mail { HYPERLINK mailto:e.cockburn-at-mluri.sari.ac.uk } {underline} =
{color} {param} 0000,0000,FF00 {/param} e.cockburn-at-macaulay.ac.uk {/underline} {=
color} {param} 0100,0100,0100 {/param} to whom completed
application forms must be returned by 20 July 2001. {/flushboth}

Quote Ref MA17/01

{FontFamily} {param} Arial {/param} Web Site { HYPERLINK http://www.mluri.sari=
.ac.uk } {color} {param} 0000,0000,FF00 {/param} http://www.macaulay.ac.uk {colo=
r} {param} 0100,0100,0100 {/param}



From daemon Wed Jul 18 02:40:32 2001



From: Alistair Smith :      a.smith-at-mluri.sari.ac.uk
Date: Wed, 18 Jul 2001 08:38:36 +0100
Subject: ELECTRON MICROSCOPIST VACANCY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




{center} {bold} {FontFamily} {param} Book Antiqua {/param} {bigger} {bigger} MACAU=
LAY INSTITUTE {/bold} {color} {param} 0100,0100,0100 {/param} {smaller} {smaller} =
{/center}


{center} {bold} {bigger} {bigger} ANALYTICAL GROUP {/center}


{center} ELECTRON MICROSCOPIST {/center}


{flushboth} {/bold} {smaller} {smaller} Applications are invited from suitably=
qualified and experienced
candidates for the above post. The successful candidate will be
responsible for providing an electron microscopy service in
support of the Institute's research programme and on a contract
basis to commercial organisations. {/flushboth}


{flushboth} Candidates should possess a degree in an appropriate subject an=
d
be experienced in the analysis of a wide range of materials by
scanning electron microscopy. {/flushboth}


{flushboth} Starting salary, depending upon qualifications and experience,
will be normally within the range =A318,500 - =A321,400 per annum
(performance related to a maximum of =A3 26,000). Non-
contributory Superannuation Scheme. {/flushboth}


MLURI receives funding from the Scottish Executive Environment
and Rural Affairs Department.


{flushboth} Further particulars and application forms can be obtained from
Personnel, The Macaulay Land Use Research Institute,
Craigiebuckler, Aberdeen, AB15 8QH Tel 01224-498200, Fax 01224-
311556, E-mail { HYPERLINK mailto:e.cockburn-at-mluri.sari.ac.uk {FontFamily} =
{param} Times New Roman {/param} } {underline} {color} {param} 0000,0000,FF00 {/pa=
ram} {FontFamily} {param} Book Antiqua {/param} e.cockburn-at-macaulay.ac.uk {/unde=
rline} {color} {param} 0100,0100,0100 {/param} to whom completed
application forms must be returned by 20 July 2001. {/flushboth}

Quote Ref MA17/01

{FontFamily} {param} Times New Roman {/param} Web Site { HYPERLINK http://www.=
mluri.sari.ac.uk } {underline} {color} {param} 0000,0000,FF00 {/param} http://ww=
w.macaulay.ac.uk {/underline} {color} {param} 0100,0100,0100 {/param} {FontFamil=
y} {param} Arial {/param}



From daemon Wed Jul 18 07:09:17 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 18 Jul 2001 08:56:41 -0300
Subject: Re: Removing Spurrs Resin from sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rose wrote:
} Hi!
} I have a question regarding the removal of Spurrs resin from sections on
} slides. I want to use aniline blue as a callose stain for plant tissue. I
} have sections (embedded in Spurrs) on slides. The protocol I have to
} remove the resin uses sodium methoxide and benzene (Mayor, Hampton and
} Rosario, 1961). I was wondering if anyone can suggest a protocol that is
} less dangerous than using the sodium methoxide. Or, alternatively, has
} anyone used this technique, or a similar one and had any problems/success
} with it?
} Thanks,
} Rose

Rose,
I use sodium ethoxy (a saturated NaOH solution in absolute ethanol) for the
Spurr removal in plant tissues. Use 5 minutes for a semithin section with
0.35 micrometers. Wash shortly in ethanol and, after, distilled water. The
technique gives good results and it does not use methanol (dangerous to your
health). I think that the protocol can be used by you without problems.
Dr. Rinaldo Pires dos Santos
E-mail: rinaldop-at-uol.com.br
Plant Anatomy Laboratory - Dept. of Botany
UFRGS - Porto Alegre - RS
Brazil



From daemon Wed Jul 18 07:24:43 2001



From: l.keddie-at-mluri.sari.ac.uk
Date: Wed, 18 Jul 2001 07:20:33 -0500
Subject: Electron Microscopist Vacancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_

ANALYTICAL GROUP


ELECTRON MICROSCOPIST


Applications are invited from suitably qualified and experienced
candidates for the above post. The successful candidate will be
responsible for providing an electron microscopy service in support
of the Institute's research programme and on a contract basis to
commercial organisations.

Candidates should possess a degree in an appropriate subject and be
experienced in the analysis of a wide range of materials by scanning
electron microscopy.

Starting salary, depending upon qualifications and experience, will
be normally within the range £18,500 - £21,400 per annum (performance
related to a maximum of £ 26,000). Non- contributory
Superannuation Scheme.

MLURI receives funding from the Scottish Executive Environment and
Rural Affairs Department.

Further particulars and application forms can be obtained from
Personnel, The Macaulay Land Use Research Institute, Craigiebuckler,
Aberdeen, AB15 8QH Tel 01224-498200, Fax 01224- 311556, E-mail {
HYPERLINK mailto:e.cockburn-at-mluri.sari.ac.uk
}e.cockburn-at-macaulay.ac.uk to whom completed application forms must
be returned by 20 July 2001.
Quote Ref MA17/01
Web Site { HYPERLINK http://www.mluri.sari.ac.uk }http://www.macaulay.ac.uk


From daemon Wed Jul 18 08:03:51 2001



From: Petersen, Maureen A. :      Mape-at-mail.ifas.ufl.edu
Date: Wed, 18 Jul 2001 08:58:07 -0400
Subject: Re: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am going to wade in on this, despite the potential for being said to be
beating a dead horse:

During a graduate virology course a few years ago it became apparent that
one of our students was pregnant. Out of concern for her and her child, we
took special interest in providing information to her, and reducing her
exposure to chemicals. We run a safe ship, but everyone should acknowledge
that during gestation a developing child is vulnerable to things the mother
may be exposed to. In this case, the students worked in pairs, and it was a
simple matter the have her partner handle chemicals of concern. Since she
was a new graduate student, we felt it was responsible to provide her with
all the information we could for her safety and her child's. Her learning
experience was not hindered, and on a personal level I think she appreciated
our concern.

Information and education are critical to learning to make responsible
decisions regarding safety, pregnant or not.


Maureen Petersen
University of Florida

****************************************************************************
**************************


-----Original Message-----
} From: Chris Jeffree [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, July 17, 2001 7:38 PM
To: Debby Sherman
Cc: microscopy-at-sparc5.microscopy.com


Hear hear!

} Safety is everyone's concern regardless of sex or biological
conditions. Whatever policies are in place should be there for
everyone, not just that occasional special situation. To have a lab
manager or supervisor demand private medical information about users
is most certainly an invasion of privacy.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-099e Whistler Building
} West Lafayette, IN 47907




From daemon Wed Jul 18 08:42:12 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Wed, 18 Jul 2001 08:30:07 -0500
Subject: Re: tissue preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Meghan Towner:

Your protocol is for immunoEM with perfusion-fixation. I think perfusion-fixation is necessary for your experiment. But if your purpose is to see the ultrastructure, you should use 2% glutaraldehyde in stead of 4% paraformaldehyde as the fixative for the primary fixation. Your protocol for the perfusion is okay. Just try gluta.

Greg Ning

Gang Ning, M.D., Ph.D.
Director
EM Facility
Medical College of Wisconsin

Meghan Towner wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello-
} I am a grad student at the University of Idaho and would like some input on a protocol for fixing rat tissue in order to view their spinal ganglia on the TEM. First heparin is injected intracardiac, then perfusion via the heart with saline (37C) followed by paraformaldehyde (4%, 15C). The vertebral column is then removed and immersed in 4% paraformaldehyde for several hours before dissecting the spinal ganglia. This protocol was given to me by my advisor, but I have been unable to find a similar method cited in the literature. Also, I have used this method and found all the mitochondria have already lost their cristae. Any suggestions and comments would be appreciated.
} Thank you.
} --Meghan Towner



From daemon Wed Jul 18 09:00:45 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Wed, 18 Jul 2001 09:55:30 -0400
Subject: Titanium Particle dispersion for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Your solution depends on what type of TiO2 you have and the particle size of the TiO2 you want to disperse. It can depend on whether you want to see ultimates, agglomerates or aggregates (primary structural units). You gave no indication of the type of TiO2 you want to disperse or if it was milled.

R900, 901, 100, &101 Dupont equivalents will have to be dispersed differently from something like micronized TiO2 (very agglomerated) or something like Nanotech TiO2.

Dispersion can depend on the TiO2 being rutile (I assume this is your type (coated)), anatase (uncoated), or a mixture of both, like Nanotech's spherical TiO2.

The technique we used for TiO2, and still use, was written up and given at the University of Michigan to the president of JEOL (Dr. Ito) and Prof. Wil Bigelow of the University of Michigan in the 1960s.

The presentation was given in the about 1963? by Robert Fear and was called A Versatile Mulling Technique (or Method) for Dispersing Fine Powders (or Pigments). Bob says it was sent to the EMSA / MSA educational archives. It was complete with colored 35mm slides. We have no idea where this material is currently at the MSA.

Paul Beauregard / Robert Fear
Sr. Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15601

Any opinions are ours and not those of PPG Industries as a whole.


-----Original Message-----
} From: Christopher M May [mailto:cmmay-at-US.ibm.com]
Sent: Tuesday, July 17, 2001 12:52 PM
To: microscopy-at-sparc5.microscopy.com


Hello,

I am working with a TEM and I would like to know the best way to disperse
Titanium Oxide particles for analysis.
I am currently using a methanol solution, and it is not working very well.


Sincerely,

Christopher May
IBM Analytical Services



From daemon Wed Jul 18 09:07:32 2001



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Wed, 18 Jul 2001 09:42:03 -0400
Subject: LM - Microscopy of Waxes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,
I am planning to complete the microscopical characterization of some
selected waxes using a hot stage on a light mcroscope. There does not seem
to be much information in the literature on this subject and I thought there
must be some microscopists well acqainted with this topic. We have completed
DSC thermograms and other than looking for melting/crystallization
temperatures, degree of crystallinity, is there anything else to be
characterized? Can anyone provide further guidance? Thanks.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com



From daemon Wed Jul 18 10:18:18 2001



From: Don Grimes :      microtoday-at-mindspring.com
Date: Wed, 18 Jul 2001 10:53:49 -0500
Subject: M&M Transportation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Readers,
A question many may be asking, how do I get from the L.A. airport to Long
Beach?
I am advised that there is a (blue) bus system called SuperShuttle which
leaves from outside the airline locations to Long Beach. Cost is $15 a head
and it takes around a half hour and, typically, makes 3 stops. The taxi fare
is around $45.
Don Grimes, Microscopy Today



From daemon Wed Jul 18 10:36:22 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 18 Jul 2001 11:31:41 -0400
Subject: RE: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't understand Greg's comment (below). Power DTSA v 2.5.1, with the
MSA 1.0 plug-in, will happily read EDAX Phoenix MSA files, which have a
single column of spectral data as integers, and Link ISIS MSA files, which
have the Y-data in 5 columns of reals. There is a tag, #DATATYPE, which,
if set to "Y", tells the system to expect just the y-data, and not data pairs.

The problem with MSA files is that the MSA file specification is not a
"format" - i.e., two people writing the same data to an MSA file can end up
with different files, each of which is completely according to the
specification. The problem for the reader is to be able to handle all the
different possibilities.

One way that you can sometimes use to force a file to read in is to use the
target system to write an MSA file (which, presumably, it will read back
in) using the same channel specifiacations (number of channels, range, etc)
as the source spectrum. Then use a text editor to copy the header from the
target spectrum to the source spectrum. Tags like the #NCOLUMNS tag can be
edited as required. Even this doesn't work if the format of the data
itself is different, or if the target system will only accept a limited
range of options (e.g. #NPOINTS must be a power of 2 in some systems), and
the source doesn't match the required option.

The MSA reader should, of course, at least exit gracefully if it can't
accept a spectrum, but not all do!

Tony Garratt-Reed.



}
} The NORAN EMSA files follow the specifications fully and support all
} varieties of EMSA options available. The problem is that DTSA only reads
} one type of EMSA file format (X,Y pairs of data) which is not the default
} condition of the VOYAGER files. The default format for VOYAGER stores only
} the channel data in 5 columns. The default file format for VANTAGE was
} changed to be X,Y pairs which is consistant with DTSA.
}
} There is a solution for VOYAGER. You can save the EMSA format compatible
} with DTSA by using the shell command:
} emsa_io -x=1 -w={path to store to} -s={spectrum name to store}
} example: emsa_io -x=1 -w=$HOME/spectra/myspec.emsa -s=spectrum1
}
} When the next version of VOYAGER software is released the default condition
} for the emsa_io program will be converted to the same as VANTAGE which will
} make VOYAGER's default version of EMSA file format consistant with DTSA.
}
} Greg Fritz
}


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Wed Jul 18 11:06:52 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 18 Jul 2001 12:01:00 -0400
Subject: RE: tissue preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Meghan,
Try almost any of the introductory books on EM by Hayat, M.A.. Your
library or advisor, or EM book shelf should have a copy. Most problems such
as you describe are due to the failure to initiate fixation in a timely
manner, the osmolarity of the vehicle, and the postfixation time and
component sequence. Most in situ, perfusion fixations include a postfix
with glutaraldehyde following the initial stabilization with HCHO (made FROM
paraformaldehyde). Also, it is my experience that one can 'freeze' thin
muscle bands with 4% HCHO at 4oC in 10 min. But one cannot satisfactorily
FIX the material for subsequent light(via paraffin) or EM (via plastic)
viewing in that time. Hayat appears to suggest 24hr postfix but also
provides a 4hr fix to block technique, so almost anything, properly
designed, can work.
Hayat, in a small methods book (HAYAT, M.A., 1972. BASIC ELECTRON
MICROSCOPY TECHNIQUES, VAN NOSTRAND REINHOLD, NEW YORK AND LONDON.)
references a method by: [Schultz, RL and Case, NM (1970), "A modified
aldehyde perfusion technique for preventing certain artifacts in electron
microscopy of the central nervous system", J Microscopy, 92:69.]
If you can't find either the book or the article, ask and I'll send
the method (not brief!)

Good luck,

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.




} ----------
} From: Meghan Towner
} Sent: Tuesday, July 17, 2001 7:38 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: tissue preparation for TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello-
} I am a grad student at the University of Idaho and would like some input
} on a protocol for fixing rat tissue in order to view their spinal ganglia
} on the TEM. First heparin is injected intracardiac, then perfusion via the
} heart with saline (37C) followed by paraformaldehyde (4%, 15C). The
} vertebral column is then removed and immersed in 4% paraformaldehyde for
} several hours before dissecting the spinal ganglia. This protocol was
} given to me by my advisor, but I have been unable to find a similar method
} cited in the literature. Also, I have used this method and found all the
} mitochondria have already lost their cristae. Any suggestions and comments
} would be appreciated.
} Thank you.
} --Meghan Towner
}
}


From daemon Wed Jul 18 11:10:51 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Jul 2001 12:06:02 -0400
Subject: RE: DTSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After fooling with EELS data stored in EMSA format from a Gatan system for a while that was stored in rows of five of the Y-only format and trying to extract the data into Excel, I wrote my visual basic program to extract the data and then write the data back in x,y pairs so that I could very easily plot it in Excel. Then I went nuts in Visual Basic 6.0 and wrote the program to plot the data and play around with it. I mentioned it before; I will bring to M&M and make public.

Before I wrote the program, I tried to extract the data with the "rows of five" problem in Excel using macro programming, but gave up. The easiest way that I found to convert the data to single column data and then add the x data was to first use a word processor and get rid of the hard returns using search and replace and add the appropriate character to match the data separator in the row. I then removed the data separators and added a hard return. Then you can save as a text file and open the data file in Excel. You then parse the data correctly and move the column of data over by one. Then you use a series fill to generate the x data. You can then plot the data using Excel (or any other plotting program) to plot the x,y pairs.

If you want to, you can easily change the data headers in the EMSA data file to reflect the change in the way the data is now stored in the file. You can go to the EMMPDL library and find the file with the standard and figure out how to do it relatively easily.

I think that the biggest problem with the EMSA format is allowing the Y-only data to exist in rows of n values. However, I understand why that was done. For the older programming languages, it was easy to format the read/write statement to bring the data in/out that way. However, it is not a major stumbling block.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Anthony J. Garratt-Reed [mailto:tonygr-at-mit.edu]
Sent: Wednesday, July 18, 2001 11:32 AM
To: Fritz, Greg; microscopy-at-sparc5.microscopy.com


I don't understand Greg's comment (below). Power DTSA v 2.5.1, with the
MSA 1.0 plug-in, will happily read EDAX Phoenix MSA files, which have a
single column of spectral data as integers, and Link ISIS MSA files, which
have the Y-data in 5 columns of reals. There is a tag, #DATATYPE, which,
if set to "Y", tells the system to expect just the y-data, and not data pairs.

The problem with MSA files is that the MSA file specification is not a
"format" - i.e., two people writing the same data to an MSA file can end up
with different files, each of which is completely according to the
specification. The problem for the reader is to be able to handle all the
different possibilities.

One way that you can sometimes use to force a file to read in is to use the
target system to write an MSA file (which, presumably, it will read back
in) using the same channel specifiacations (number of channels, range, etc)
as the source spectrum. Then use a text editor to copy the header from the
target spectrum to the source spectrum. Tags like the #NCOLUMNS tag can be
edited as required. Even this doesn't work if the format of the data
itself is different, or if the target system will only accept a limited
range of options (e.g. #NPOINTS must be a power of 2 in some systems), and
the source doesn't match the required option.

The MSA reader should, of course, at least exit gracefully if it can't
accept a spectrum, but not all do!

Tony Garratt-Reed.



}
} The NORAN EMSA files follow the specifications fully and support all
} varieties of EMSA options available. The problem is that DTSA only reads
} one type of EMSA file format (X,Y pairs of data) which is not the default
} condition of the VOYAGER files. The default format for VOYAGER stores only
} the channel data in 5 columns. The default file format for VANTAGE was
} changed to be X,Y pairs which is consistant with DTSA.
}
} There is a solution for VOYAGER. You can save the EMSA format compatible
} with DTSA by using the shell command:
} emsa_io -x=1 -w={path to store to} -s={spectrum name to store}
} example: emsa_io -x=1 -w=$HOME/spectra/myspec.emsa -s=spectrum1
}
} When the next version of VOYAGER software is released the default condition
} for the emsa_io program will be converted to the same as VANTAGE which will
} make VOYAGER's default version of EMSA file format consistant with DTSA.
}
} Greg Fritz
}


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Wed Jul 18 11:42:41 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 18 Jul 2001 11:36:19 -0500
Subject: Sad News: Passing of Dr. Lonnie Russell

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a brief note to inform the microscopy community of the death of
Dr. Lonnie Russell on July 11. Dr. Russell drowned while swimming in
the ocean off the coast of Brazil. Lonnie made significant
contributions in microscopy (both light and electron) and was an
internationally recognized expert in the area of reproductive
biology. I have more information about Lonnie, so please contact me
personally and I will forward it to you.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Jul 18 12:28:15 2001



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Wed, 18 Jul 2001 13:24:44 -0500
Subject: RE: DTSA and EMSA format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} The problem with MSA files is that the MSA file specification is not a
} "format" - i.e., two people writing the same data to an MSA file can end up
} with different files, each of which is completely according to the
} specification. The problem for the reader is to be able to handle all the
} different possibilities.
}

The problem we have in implementing a robust MSA file reader is lack of
example files from the various MSA file writers. Because there are several
flavors in implimenting the writer, many programmers take different paths
which should not make a difference to a MSA file reader. When programming
the MSA file writer, one sometimes does not think of the other
possibilities. It's only when users complain that they cannot read a MSA
file from some other system that the problems become known. Then resources
have to be allocated to fix a problem that should have been found during
the testing phase of the software. Does not seem to be hard but this can be
costly (from a company viewpoint).

Something that would help this oversight would be a repository for the
various MSA file flavors, something one could download to use as a test
case for the robustness of their MSA file reader. The spectral content
could be anything but should be real (AlCu calibration for example).

We would be happy to sponser such a location on our web site.

Scott


-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Jul 18 14:20:01 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 18 Jul 2001 15:12:35 -0400
Subject: RE: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: It Adams (My Employer),

I thought I would forward an example of the kind of madness to which
one may be driven by the need to avoid liability in the workplace. The
gentleman who formulated the list at the base of this forwarded email had
earlier requested information about how others handled pregnancy among
workers in electron microscopy laboratories. He then reported his solution
as you can see below.

I am inserting without comment a statement from our lawyers, Dumb,
Dumber and Dumbest, Esquires, LTD., LLD, LLC, LLB, LLA, LZ, LY, which they
returned in response to a question about my potential liability on this
matter.

"Understanding that most exposures to hazard occur as a consequence
of accident or personal negligence, we have come to the conclusion that it
is impossible, if not illegal, to protect anyone in particular, much less
someone who is specifically pregnant, from exposure to hazardous materials,
especially if the individual is going to take a job that involves such
hazard and continue to do THAT job during pregnancy. Thus, it behooves the
employer to remove such hazards completely or to reduce them for those at
greatest risk so that there is no risk to all, especially those who
periodically enter periods of existence during which their risk increases.
However, we must state that for us this is a matter of humane treatment of
subordinates, especially those weak in mind if not in body, not the law,
which in some measure is inscrutable, even to us.

The legal intricacies of this matter in particular border on the
insane, and force us to adopt unusual and novel measures and reasoning to
protect both the employer and its employees. It appears that the Supreme
Court in one of its more enlightened decisions, ruled that a company may NOT
discriminate among its employees in the matter of hazardous substance risk.
Thus, a battery manufacturer was not permitted to protect pregnant employees
by transfer away from lead, when it did not similarly protect the remainder
of the work force. In fact, this ruling is quite reasonable given the fact
that during the first trimester of pregnancy, the Supreme Court had already
ruled that pregnancy, especially during the period mentioned, is in NO
respect the business of Society (nor, by any extension, management).

Therefore, if during the first trimester, an accident might occur to
damage that which is none of Society's business, there is no recourse set
aside for management that can protect that which is none of ITS business
during that period. We can all, however, assure the employer that should a
pregnant employee decide to continue a pregnancy beyond the first trimester,
the employer becomes wholly responsible for any damage that occurred to the
object of the pregnancy during the time that the object of the pregnancy was
none of its business. It is a quirk of this situation that the object of
the pregnancy has no legal standing either during this period, when it is
none of Society's or management's business, or, for that matter, during the
entire duration of pregnancy. Thus, the employer is uniquely at risk of
inflicting damage on an object with no legal standing during a period when
that object is none of the employer's business and, legally, does not exist.
Of course, one might argue, just as logically, that it is not possible to
inflict harm on something that does not exist, but that avoids the practical
reality. We continue to expand this reasoning for the purposes of expected
appeals should our position prove, in the first instance, legally
unreasonable (charges, as stipulated, per hour).
The only recourse may be a standard agreement to be signed by ALL
employees, that ANY who become pregnant (through physical or ethereal means,
regardless of anatomy or lack thereof exhibited by themselves or others
related or unrelated to the acts involved or uninvolved, philosophy,
political party, voting record, religion, non-religion, sexual disposition
or preference, or sexual non-preference or non-disposition, or any absence
or presence of preference or disposition and notwithstanding the expected or
unexpected effects of genetic predisposition or specific incapacity or
capacity) will agree to be transferred to non-hazardous, riskless,
activities for the course of that pregnancy, nominally nine months from the
moment of conception. If this solution is adopted by the employer, then the
employer can be assured that such actions will protect those offspring of
employees who have or will have legal standing and for whose well-being the
employer can ever be held responsible, if someone decides that such
well-being was or is the employer's business.

Since discrimination against the parent is not permitted, it is
obvious that all individual distinctions must be suppressed in any safety
considerations and actions. For those who refuse such PROTECTION during
pregnancy, patently hazardous employment should not be available at any time
during which pregnancy (through physical or(see above)....), may or might be
improbably possible. The other side of the coin is that all employees,
regardless of job, must be treated identically. Thus once safety
considerations have been installed in equal consideration of all, all
employees will be able and free to enter, with equanimity, their job space
by first travelling through those non-discriminating, hazardous, but equally
safe and non-hazardous work spaces.

The reasoning for the above solution is simple and straight forward.
Since there is no absolute evidence to suggest that true hermaphroditism is
not possible in humans, it is reasonable for the employer to subject ALL
employees to the above non-discriminatory employment process during the
pre-employment interview as well as after employment. The employer, thus,
has assumed the responsibility to dictate what it considers hazardous and
potentially harmful to this broad group of individual employees. This is
reasonable since the employer is at risk for damage to the potential
offspring during times when the potentially pregnant employee may bring the
potential offspring to the place of employment when such pregnancy is none
of the employer's business (but when the employer's potential risk is real).
It is not that the employer has rights nor can be said to have
responsibility during the time of pregnancy when such pregnancy is none of
its business, but the employer can claim the right to protect itself, when
the potential offspring, during the time that when its existence is none of
the employer's business, is exposed to hazards on the employer's property
that may cause harm to the potential offspring when the parent decides to
continue the pregnancy beyond that period when it is none of Society's or
any employer's business, such that the potential offspring is permitted to
continue to develop in such manner that it can potentially claim rights of
redress for harm done during that time of its non-existence when it was
exposed to hazard when such exposure, by simple logic, was none of the
employer's business, and concurrently could not have happened."

It should be clear that the solution recommended by our legal team
treats all potential employees in the same potential manner, giving all
their potential rights according to Supreme Court ruling and protecting all
equally without potential discrimination. Thus, reason, not chaos, will
prevail!

Please pardon any errors in the above. I have not had time to
review it since it was returned, a second time, edited for clarity and
brevity, from the lawyer's office.

Since I cannot, in good conscience, ignore the above reality, I am
obliged to pass this information on to you, my superiors, who will also be
forced to become 'part of the problem' rather than 'part of the solution'.
My apologies to all - indiscriminately! To those pregnant employees who
insist that hazardous work must continue without hazard and, thus, constrain
their employers to liquidate the workplace, I accord you the sensitivity and
concern that I normally reserve for the incoherent. I may wonder what you
are saying, but I never try to understand what you are thinking.

Fred Monson, who wouldn't suffer his wife, and thus, their unborn children,
to work in the same business as he, but whose daughter has freely chosen it.

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: William McManus
} Sent: Tuesday, July 10, 2001 11:30 AM
} To: Leona Cohen-Gould; Monson, Frederick C.; Sally Stowe
} Cc: Microscopy Listserver
} Subject: lab pregnancy
}
} to all out there:
}
} This is what I have done so far:
} 1. Removed all stored chemicals from the lab.
} 2. Moved all chemical mixing into fume hood.
} 3. Met for 3 hours with environmental safety officer.
} 4. Met for 2 hours with radiation safety officer.
} 5. Eliminated all protocols which involve teratogens from pregnant
} workers routine.
} 6. Established a personal hygiene plan for pregnant worker, which is
} being signed of by worker, myself, director of the lab, safety
} officer, and her physician.
} 7. Reiterated safety protocols for worker, coat , gloves, safety
} glasses, etc.
} 8. Had lab monitored for radiation, passed.
} 9. Had lab monitored for formaldehyde gas, passed.
} 10. Planning to monitor xylene exposure to worker when thin sectioning
} (cannot do this in the hood, obviously). Heat pen( modified
} soldering
} iron) has not worked well so far, any ideas how to eliminate
} xylene?
} 11. Still looking for a non toxic general 1 micron stain for Spurrs and
} LR White. Any ideas?
} I am experimenting with food related stains (grape juice, etc.).
} 12. Employee has completed, lab safety training course, formaldehyde
} training course and radiation training course.
} 13. Will continue to monitor lab for chemical and radation exposure.
}
} To some this may seem like over kill, but I believe that it is very
} important that every stone is turned in regards to safety. Another
} issue that I face is that I have numerous grad students coming through
} the lab, and there is always a possibility that they are pregnant and do
} not realize it or keep it from me. I also have a nursing mother who was
} visiting the lab and never bothered to tell me, until the subject of why
} the lab was reorganized came up. I now demand that all female student,
} techs, visitors, ect.
} tell me of their "motherhood" status.
}
} Bill
}
} -----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
} Sent: Tuesday, July 10, 2001 7:28 AM
} To: William McManus; Monson, Frederick C.; Sally Stowe
} Cc: Microscopy Listserver
} Subject: RE: Tol blue/borax
}
}
} At 1:04 PM -0600 7/9/01, William McManus wrote:
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} .
} }
} }
} } The issue of pregnant (or possibly pregnant) workers in the lab has
} } turned out to be fairly complicated. If anyone else out there has beem
} } through this situation, I would like to know how you dealt with it.
} }
} } Bill
} }
} } William McManus
} } Supervisor
} } Electron Microscopy Facility
} } Department of Biology
} } Utah State University
} } Logan UT 84322-5305
} }
} ****************
} to Bill & All,
}
} When I was pregnant, 9 years ago, I was the entire staff of the EM
} facility here (still was until a month ago)and so could not avoid
} things. The way I handled safety concerns was to be extra cautious:
} I worked in the hood, wore double gloves when working with fixatives
} & resins and gloves for other times when i would not usually wear
} them( like using Tol Blue), safety glasses if splashing was a
} possibility, washed my hands almost compulsively. In short, I used
} common sense. I was, and am, lucky that (UrAc aside) I don't have to
} deal with radioactivity. colleagues of mine who were also pregnant
} at the time (we had a rash of pregnancies that year) who did need to
} work with isotopes minimized their use, got new shields, and at times
} convinced labmates to do those steps for them!
}
} We all, thankfully had healthy babies who are now all approaching
} adolescence and driving us nuts, but that's another issue for another
} forum!
}
} Lee
}
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}


From daemon Wed Jul 18 15:17:06 2001



From: Beth Maguire :      BMaguire-at-nemours.org
Date: Wed, 18 Jul 2001 16:12:30 -0400
Subject: filter cubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there a broad range spectra cube set that you can recommend for a zeiss
axioskop rather than buy individual cubes?
Thanks

BMaguire
email:bmaguire-at-nemours.org



From daemon Wed Jul 18 15:42:53 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Jul 2001 16:36:54 -0400
Subject: RE: DTSA and EMSA format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,
I have a couple of them on my disk and that is included in my program distribution files. I am going to put it in the MSA and MAS libraries. I am bringing it to M&M and I will give you a copy. They include Gatan, Noran, Emispec, and one other that I can't think of at the moment (I think that it was Oxford).

I had asked for examples and I got a few to try in the program.

If anyone would like to send me one of their EMSA formatted ASCII output EDS or EELS spectra files and tell me the vendor associated with source, I could include them with the distribution files.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Scott D. Davilla [mailto:davilla-at-4pi.com]
Sent: Wednesday, July 18, 2001 2:25 PM
To: Microscopy (E-mail)


} The problem with MSA files is that the MSA file specification is not a
} "format" - i.e., two people writing the same data to an MSA file can end up
} with different files, each of which is completely according to the
} specification. The problem for the reader is to be able to handle all the
} different possibilities.
}

The problem we have in implementing a robust MSA file reader is lack of
example files from the various MSA file writers. Because there are several
flavors in implimenting the writer, many programmers take different paths
which should not make a difference to a MSA file reader. When programming
the MSA file writer, one sometimes does not think of the other
possibilities. It's only when users complain that they cannot read a MSA
file from some other system that the problems become known. Then resources
have to be allocated to fix a problem that should have been found during
the testing phase of the software. Does not seem to be hard but this can be
costly (from a company viewpoint).

Something that would help this oversight would be a repository for the
various MSA file flavors, something one could download to use as a test
case for the robustness of their MSA file reader. The spectral content
could be anything but should be real (AlCu calibration for example).

We would be happy to sponser such a location on our web site.

Scott


-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Jul 18 15:44:15 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 18 Jul 2001 16:40:31 -0400
Subject: Re: tissue preparation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Meghan Towner wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hello-
} } I am a grad student at the University of Idaho and would like some input on a protocol for fixing rat tissue in order to view their spinal ganglia on the TEM. First heparin is injected intracardiac, then perfusion via the heart with saline (37C) followed by paraformaldehyde (4%, 15C). The vertebral column is then removed and immersed in 4% paraformaldehyde for several hours before dissecting the spinal ganglia. This protocol was given to me by my advisor, but I have been unable to find a similar method cited in the literature. Also, I have used this method and found all the mitochondria have already lost their cristae. Any suggestions and comments would be appreciated.
} } Thank you.
} } --Meghan Towner

If you want good ultrastructure use gluatarladehyde in the fixative, as little as 1% will make a significant improvement but 2-3% is more common. The buffer used is also important, you did not specify which you are using. Paraformaldehyed alone can give good ultrastructure but you will need to fix for several days at a minimum. If you want to do immunostaining, you may have to skip the glut. and suffer with less than optimal ultrastructure or risk killing off the immunoreactivity. It will depend on the antigen in question.
Also, the temperature shift from 37 degrees C for the washout to 15 degrees C for the fixative is likely to induce vasospasm, reducing the amount of fixative delivered to the tissue. Do everything at one temperature, room temperature should be fine.
A good basic EM text will be invaluable. Brenda Weakley's "Beginners handbook in biological transmission electron microscopy" is excellent. "Biological techniques in electron microscopy" by Clinton Dawes is also very good but it may be out of print.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Jul 18 15:44:26 2001



From: Jan Markham :      afp042-at-email.sps.mot.com
Date: Wed, 18 Jul 2001 13:39:36 -0700
Subject: Re: Fwd: lab pregnancy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Safety is everyone's concern regardless of sex or biological conditions. Whatever policies are in place should be there for everyone, not just that occasional special situation. To have a lab manager or supervisor demand private medical information about users is most certainly an invasion of privacy.
} Debby

While Debby's basic premise is correct, it is also a fact that a fetus
can be adversely affected by chemicals, or levels of chemicals, that
would pose absolutely no threat to adults. I have worked in situations
where implementation of all the precautions necessary to prevent such
fetal exposure would have made the job impossible. We acknowledge these
differences when approving new drugs for the market, and restrict their
usage accordingly. Is the lab situation so very different?

Jan Markham


From daemon Wed Jul 18 16:08:27 2001



From: Dennis C. Winkler :      Dennis_Winkler-at-nih.gov
Date: Wed, 18 Jul 2001 17:02:57 -0400
Subject: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Good Day All!

In laboratories where large numbers of EM micrographs are produced
over the years and researchers may come and go over time, what system
do you use to keep track of the micrographs?

We were thinking of implementing a computer database where
information about each micrograph (such as date, microscopist,
sample, conditions, location of the archived micrograph, etc.) can be
stored for future reference. Is there any existing database software
(preferably Macintosh based) for this purpose or any opinions on such
packages?

Thanks for your help.

- Dennis

------------------------------------------------------------------------
Dennis C. Winkler, PhD. Phone: (301) 496-0131
Laboratory of Structural Biology Research Fax: (301) 480-7629
NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
Bldg. 6, Room B2-26, MSC-2717
Bethesda, MD 20892-2717, U.S.A.


From daemon Wed Jul 18 16:38:03 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 18 Jul 2001 17:32:18 -0400
Subject: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Thumbsplus from Cerious software. (www.cerious.com) PC based. I don't know if they ever came out with the Mac based version, but they did have a beta version at one time.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Dennis C. Winkler [mailto:Dennis_Winkler-at-nih.gov]
Sent: Wednesday, July 18, 2001 5:03 PM
To: Microscopy-at-sparc5.microscopy.com



Good Day All!

In laboratories where large numbers of EM micrographs are produced
over the years and researchers may come and go over time, what system
do you use to keep track of the micrographs?

We were thinking of implementing a computer database where
information about each micrograph (such as date, microscopist,
sample, conditions, location of the archived micrograph, etc.) can be
stored for future reference. Is there any existing database software
(preferably Macintosh based) for this purpose or any opinions on such
packages?

Thanks for your help.

- Dennis

------------------------------------------------------------------------
Dennis C. Winkler, PhD. Phone: (301) 496-0131
Laboratory of Structural Biology Research Fax: (301) 480-7629
NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
Bldg. 6, Room B2-26, MSC-2717
Bethesda, MD 20892-2717, U.S.A.


From daemon Wed Jul 18 18:41:59 2001



From: Christopher M May :      cmmay-at-US.ibm.com
Date: Wed, 18 Jul 2001 11:44:07 -0400
Subject: Clarification of Titanium Particle dispersion for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Thanks for your input, but let me clarify. I am working with Nanotech
TiO2, I am looking at sizes 100nm and smaller. The particles are spherical
and what I would eventually like are measurements of their diameters which
reflects as accurate a distribution as I can get. I do not want
aggolmerates, just the particles. Therefore, any technique that has a
selection bias I would rather not use.

Thanks again,
Christopher May
IBM Analytical Services


----- Forwarded by Christopher M May/Poughkeepsie/IBM on 07/18/2001 11:38
AM -----

"Christopher M
May" To: microscopy-at-sparc5.microscopy.com
{cmmay-at-US.ibm. cc:
com} Subject: Titanium Particle dispersion for TEM

07/17/2001
12:51 PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am working with a TEM and I would like to know the best way to disperse
Titanium Oxide particles for analysis.
I am currently using a methanol solution, and it is not working very well.


Sincerely,

Christopher May
IBM Analytical Services








From daemon Thu Jul 19 02:03:52 2001



From: Bernhard Sabel :      Bernhard.Sabel-at-medizin.uni-magdeburg.de
Date: Wed, 18 Jul 2001 08:47:58 +0200
Subject: Big Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My name is Professor Sabel. Whoever you are, your email is being
automatically sent to my account. I think you have a big problem
somewhere. Please check.

Regards

B. Sabel



From daemon Thu Jul 19 07:32:12 2001



From: Nick SCHRYVERS :      schryver-at-ruca.ua.ac.be
Date: Thu, 19 Jul 2001 14:21:53 +0200
Subject: EELS post doc in Antwerp, Belgium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

please find below the announcement for a long-term
EELS post-doc position at the group of Electron Microscopy for Materials

Science (EMAT) in Antwerp, Belgium
http://www.ruca.ua.ac.be/emat/. May I ask you to distribute this to
colleagues that may be interested.

Many thanks,

Nick Schryvers

EELS Post-doc position at EMAT, Antwerp, Belgium

A new post-doctoral position will be available as of January 1, 2002, at

the laboratory of Electron Microscopy for Materials Science (EMAT) in
Antwerp, Belgium. The emphasis of the work will be on the combination of

EELS with HRTEM, STEM and EFTEM with the aim to develop advanced
detection, processing and interpretation procedures yielding improved
resolutions. The work will be performed in the framework of a joint
program including a research group on image processing (VISIE) and one
specialised in spectral analysis techniques (MiTAC), both from the same
university. The ideal candidate should have an extensive experience in
performing high precision EELS experiments in the TEM. The experiments
will be conducted with two EELS systems (GIF 200(0)), one installed on a

CM30 FEG Ultratwin, designed for ultimate resolution of 0.1nm (using
Focus Variation) and another one on a 3000F JEOL FEG machine equipped
with a STEM unit with a probe size of 0.4 nm, EDX and FasTEM. At first
model systems in the field of CMR and superconducting materials,
semiconductor precipitates and diamond like carbon films will be
investigated, but the aim is for generic principles useful for a large
majority of materials.
The position is in principle for one year, but can be extended by one
year periods up to a total of 4 years.
Salary is arranged following European and local standards and includes
the full range of social security benefits (health care, retirement,
holiday, …). Actual figures depend on proven experience of the
candidate.
Please send your extensive resumé to

Prof. Dr. Dominique Schryvers
Senior Lecturer
Electron Microscopy for Materials Research (EMAT)
Department of Physics
University of Antwerp, RUCA
Groenenborgerlaan 171
B-2020 Antwerp, Belgium
Tel: 32-3-2180247
Fax: 32-3-2180257
E-mail: schryver-at-ruca.ua.ac.be, nick.schryvers-at-ua.ac.be





From daemon Thu Jul 19 08:31:48 2001



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 19 Jul 2001 09:27:25 -0400
Subject: RE: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ThumbsPlus is good if you have a digital image to keep track of, but I am
not sure if Dennis was referring to that or to physical micrographs. If it
is the latter then I would suggest using Microsoft Access and setting up
their own forms and reports to suit their particular needs.
Greg Erdos

At 05:32 PM 7/18/2001 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Ditrector, ICBR EM CORE
University of Florida Ph. 352-392-1295
PO Box 118525 Fax 352-846-0251
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl


From daemon Thu Jul 19 08:42:28 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Thu, 19 Jul 2001 14:37:37 +0100
Subject: Removing DPX/cover glass

Contents Retrieved from Microscopy Listserver Archives
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Greetings from Plymouth, UK

Can anyone advise on removing a coverslip mounted using DPX? I am currently soaking it in xylene, but overnight didn't "cut the mustard"! Any cogent comments? Please?!

Keith Ryan
Marine Biological Association
Plymouth



From daemon Thu Jul 19 08:51:46 2001



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Thu, 19 Jul 2001 09:32:19 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: Re: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I think the key here is (from the post below):

"No one should be allowed to expose themselves or others to dangers and it is THEIR RESPONSIBILITY to provide data to their managers if such a case exists or might exist."

If someone makes a medical condition known, ANY medical condition, to their supervisor, then it seems that the supervisor should take appropiraite additional action OVER AND ABOVE THE ROUTINE SAFETY PRECAUSTIONS to provide additional information and safety as required.

However, the question is not if we should attempt to make an environment as safe as possible (that does depend on specific individual need and may or may not be sufficiently possible in all situations) but whether a supervisor has the right to demand medical information from someone who may not be willing to share it. Do we have the right to not only demand the information but, upon hearing it, require the employee to observe restrictions when they, after being fully informed of hazards, etc, do not wish to do so.

Also, although this thread has dwelt on pregnancy as the issue of concern, it does take two to tango and concern for healthy sperm is as important as healthy eggs. Do we really know effects, limits, etc on all potential hazards in our labs to these tissues as well as to fetuses?

We can discuss this all day but, not being legal professionals, our thoughts are not necessarily sufficient in a court of law. I think it behooves all of us to provide as safe an environment as possible within reason and then respond to special situations in a way that is agreeable to all parties involved. This may require restrictions or reassignment of personnal if the environment cannot be made as safe an an employee/employer feels necessary (which will no doubt raise all kinds of additional questions/concerns). We are our brother's keepers only to the extent that they want to be kept!

Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907

--------------------------------------


Dennis,

You've probably already seen the 'Access' part of the MS Office programs
as a database, but if not, I use it to keep track of the admin things in
our microscopy unit and it works well, has lots of features. Another
program that could be useful, although not exactly what you're looking
for, is IrfanView, which lets you open up fast all the images you have in
a folder, like a contact print of photos - later you can do things like
copy, delete etc. on the files. It's freeware and you can download at

http://www.ryansimmons.com/users/irfanview/english.htm

Both of these I've found really useful in general organization of the data
and files that end up accumulating everywhere.

Good luck!

Mark









********************************************
Mark West,
Encargado, (Manager)
Unidad de Microscopia(Microscopy Unit),
Lab, 301-Sur,
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
Circuito Exterior, Ciudad Universitaria,
Coyoacan, C.P. 04510,
Mexico D.F.,
MEXICO

tel (unidad/lab) (525) 622 5610
(casa/home) (525) 619 3020
Fax (525) 616 2282

Pagina de Web (Web page)
http://ifcsun1.ifisiol.unam.mx/microscopy.html
********************************************

On Wed, 18 Jul 2001, Dennis C. Winkler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Good Day All!
}
} In laboratories where large numbers of EM micrographs are produced
} over the years and researchers may come and go over time, what system
} do you use to keep track of the micrographs?
}
} We were thinking of implementing a computer database where
} information about each micrograph (such as date, microscopist,
} sample, conditions, location of the archived micrograph, etc.) can be
} stored for future reference. Is there any existing database software
} (preferably Macintosh based) for this purpose or any opinions on such
} packages?
}
} Thanks for your help.
}
} - Dennis
}
} ------------------------------------------------------------------------
} Dennis C. Winkler, PhD. Phone: (301) 496-0131
} Laboratory of Structural Biology Research Fax: (301) 480-7629
} NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
} Bldg. 6, Room B2-26, MSC-2717
} Bethesda, MD 20892-2717, U.S.A.
}



From daemon Thu Jul 19 09:43:02 2001



From: Williams, David (DM) :      dmwilliams-at-dow.com
Date: Thu, 19 Jul 2001 10:38:33 -0400
Subject: CryoSEM / CT1500 with Chromium target help

Contents Retrieved from Microscopy Listserver Archives
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I have a JEOL 6320 FEGSEM equipped with a Oxford CT1500 cryo unit which I
have used for a couple of years. I recently replaced the Cr foil/al back
plate target with a Cr sputtered on aluminum target. Now I get a plasma but
no deposition of Cr. (And yes, the target is facing the right direction).
Yesterday, I replaced a leaky argon supply valve and metering valve. Still
no deposition. I have also tried a full range of current/back pressure
settings with no luck. Anyone have any ideas as to what the problem is?

The work is piling up so I am also looking for access to a FEGSEM with a
CT1500 or CT2500 cryo unit equipped with a Cr target to get some high
priority / high resolution work done. (Preferably somewhere here in the
mid-west). Please contact me with the appropriate information.

Thanks,
Dave Williams
The Dow Chemical Company
Midland, MI
dmwilliams-at-dow.com


From daemon Thu Jul 19 10:37:07 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 19 Jul 2001 11:36:30 -0400
Subject: Child care solutions for M & M 2001 - Summary

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists:

Thanks for all the responses to my search for child care options in Long
Beach. For those who wanted to know (and anyone else who is also
interested), here's what I found:

The Westin can arrange babystitting, estimated $ 15 - $ 20 per hour.

The Growing Years (1 World Trade Ctr # 199) was recommended. It has been
re-named, but can look after children up to 5, telephone (562) 495-6300
(unfortunately not good for me - my child is 6)

Affordable Nannies & Care Givers, (562) 988-6588, is an agency which
provides babysitting; usual charges $ 8 - $ 10 per hour plus fee of $ 25
per day.

If you are YMCA members, you can sign up for a few days camp at the YMCA,
1720 N Bellflower Blvd, (562) 96-3394 - it's called 'Sunshine Camp" and
costs $ 105 for 1 to 3 days, $ 140 for 4 or 5 days, plus $ 35 registration
fee; you need to go there first (i.e. Saturday or earlier) with vaccination
records etc.

Using switchboard (www.switchboard.com) to search for businesses (select
"child care services" from the list) gave me about 65 hits. One or two day
care businesses said they could provide a few days' care, but most only go
up to age 5.

Thanks again,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Thu Jul 19 11:41:35 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 19 Jul 2001 09:34:41 -0700
Subject: Workshop at M&M '01

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Project MICRO (Microscopy In Curriculum - Research Outreach) is MSA's
middle school educational outreach program. MICRO frequently sponsors
programming at the annual M&M meeting - but because it doesn't fit the
basic symposium structure, sometimes it "falls thru the cracks" in program
preparation. That happened this year; the MICRO workshop isn't listed in
the daily program. Although it's primarily intended for local teachers, it
may interest you too.

Repairing School Microscopes: A Workshop for Teachers and Volunteers
Tuesday, August 7
10 am - 3 pm, room 203C
Led by David Bentley, University of Arizona

If you ask teachers what kind of help they need, a common response is "fix
our microscopes"! In this context, "repair" means basic maintenance, rather
than things requiring expert attention, and Dave has considerable
experience in teaching the subject to teachers. If you want to help with
educational outreach but you don't want to be a classroom volunteer, come
to this workshop and learn how to help teachers in your local schools.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu Jul 19 12:54:02 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Thu, 19 Jul 2001 13:45:10 -0400
Subject: microscopy manual

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Fellow Microscopists :

We have inherited a Leitz Metallux 3 microscope with epifluorescence, however, without a user's manual (not a complaint, just a fact) and are wondering whether anyone knows a source for old microscope manuals. There are some quirky things about it, for example, the transmitted light is either yellow or red, never white, and, other than dismantling the entire body, and physically removing the filters, I cannot find a way to fix that. I could take it apart but I'm sure there would be parts left over after reassembly.

Thanks for any help.


Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Thu Jul 19 13:44:03 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Thu, 19 Jul 2001 14:39:08 -0400 (EDT)
Subject: Re: Clarification of Titanium Particle dispersion for TEM

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OK, here you go -- SOP of TEM drop grid preparation for 100nm down or up
TiO2 particles:

1) Dilute TiO2 solution to about 1% with DIW or other solvents. I have the
luck to use straight H2O in most of the cases involving particles
including TiO2. Surfactants sometimes cause more problems than resolve
particles. Alcohol or acetone could be good solvents too
2) Sonicate the solution
3) Use a pair of negative tweezers, and pick up a 200 mesh Cu C-film grid
WITH Cu-SIDE UP. You should catch as less edge of the Cu grid as possible.
4) Lay the tweezers on a table and place a quadrant of filter paper
underneath the grid/tweezes.
5) Use a pipette to transfer a drop of the liquid onto the grid. Wick
away extra liquid with half of that quadrant of the filter paper
underneath the tweezes
6) Let it dry naturally or put under a light
7) Check with TEM
8) Later, experience will tell you how much concentration to use and how
much liquid to drop onto the grid

*********************************
Chaoying Ni
Electron Microscopy Laboratory
Materials Science and Engineering
College of Engineering
University of Delaware
Newark, DE 19716
*********************************

On Wed, 18 Jul 2001, Christopher M May wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} Thanks for your input, but let me clarify. I am working with Nanotech
} TiO2, I am looking at sizes 100nm and smaller. The particles are spherical
} and what I would eventually like are measurements of their diameters which
} reflects as accurate a distribution as I can get. I do not want
} aggolmerates, just the particles. Therefore, any technique that has a
} selection bias I would rather not use.
}
} Thanks again,
} Christopher May
} IBM Analytical Services
}
}
} ----- Forwarded by Christopher M May/Poughkeepsie/IBM on 07/18/2001 11:38
} AM -----
}
} "Christopher M
} May" To: microscopy-at-sparc5.microscopy.com
} {cmmay-at-US.ibm. cc:
} com} Subject: Titanium Particle dispersion for TEM
}
} 07/17/2001
} 12:51 PM
}
}
}
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} I am working with a TEM and I would like to know the best way to disperse
} Titanium Oxide particles for analysis.
} I am currently using a methanol solution, and it is not working very well.
}
}
} Sincerely,
}
} Christopher May
} IBM Analytical Services
}
}
}
}
}
}
}









From daemon Thu Jul 19 13:44:03 2001



From: Dennis C. Winkler :      Dennis_Winkler-at-nih.gov
Date: Thu, 19 Jul 2001 14:37:41 -0400
Subject: RE: Database of Archived EM Micrographs

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Greg and others,

Clarification: Yes, I am talking about physical micrographs
(film/negatives). Sorry about the confusion.

Thanks for all of the suggestions thus far. My first thought was to
set up a FileMakerPro database to store the information, but I will
look at the commercial packages that have been mentioned.

- Dennis

------------------------------------------------------------------------
Dennis C. Winkler, PhD. Phone: (301) 496-0131
Laboratory of Structural Biology Research Fax: (301) 480-7629
NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
Bldg. 6, Room B2-26, MSC-2717
Bethesda, MD 20892-2717, U.S.A.


At 9:27 AM -0400 7/19/01, Greg Erdos wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ThumbsPlus is good if you have a digital image to keep track of, but
} I am not sure if Dennis was referring to that or to physical
} micrographs. If it is the latter then I would suggest using
} Microsoft Access and setting up their own forms and reports to suit
} their particular needs.
} Greg Erdos
}


From daemon Thu Jul 19 14:34:13 2001



From: mibuckett-at-mmm.com
Date: Thu, 19 Jul 2001 14:26:13 -0500
Subject: Re: Titania dispersion for TEM analysis

Contents Retrieved from Microscopy Listserver Archives
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We've found that it's also helpful to use a nonpolar solvent - such as
hexane or heptane - to disperse the particles in.

Mary I. Buckett
Corporate Analytical Technology Center
3M Co.
St. Paul, MN 55144





From daemon Thu Jul 19 14:34:13 2001



From: Julian Martinez-Fernandez :      Julian.Martinez-Fernandez-at-grc.nasa.gov
Date: Thu, 19 Jul 2001 15:26:56 -0400
Subject: BSE detector for a Philips XL30

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Dear colleges:

Which would be the best BSE detector to install in a Philips XL30
SEM? We want to have the maximum compositional contrast.

I would appreciate to have your input.

Thanks,
Julian



From daemon Thu Jul 19 22:25:36 2001



From: Williams, David (DM) :      dmwilliams-at-dow.com
Date: Thu, 19 Jul 2001 22:17:36 -0500
Subject: CryoSEM help

Contents Retrieved from Microscopy Listserver Archives
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{x-charset windows-1252} } I have a JEOL 6320 FEGSEM equipped with a
Oxford CT1500 cryo unit which I
} have used for a couple of years. I recently replaced the Cr foil/al back
} plate target with a Cr sputtered on aluminum target. Now I get a plasma
but
} no deposition of Cr. Yesterday, I replaced a leaky argon supply valve and
} metering valve. Still no deposition. Anyone have any ideas as to what the
} problem is?
}
} I am also looking for access to a FEGSEM with a CT1500 or CT2500 cryo
unit
} equipped with a Cr target to get some high priority work done. (Preferably
} somewhere here in the mid-west). Please contact me with the appropriate
} information.
} Thanks,
}
} Dave Williams
} dmwilliams-at-dow.com
{/x-charset}


From daemon Thu Jul 19 22:25:36 2001



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Thu, 19 Jul 2001 22:18:21 -0500
Subject: Sectioning a crystalline powder sphere

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--
To Listers,

I am a graduate student at the university of Texas Health Science Center.
As part of a project I wish to perform a spectral analysis of a
certain medication. The medication is a crystalline powder in the
form of small (approx. 1mm diameter) spheres. I want to section the
spheres to 10 to 30 microns so as to obtain a spectral analysis of
the entire bead from its outer coating to its interior. I have tried
OCT embedding and cyrostat sectioning. However, the beads pulverized
during sectioning. Can anyone recommend a way to section these
spheres so as to preserve the material as much as possible? I should
also point out that the material is freely soluble in ethanol and
methanol, as well as water.

Thank you in advance for your help and suggestions.


From daemon Thu Jul 19 22:42:12 2001



From: dkray-at-siu.edu ()
Date: Thu, 19 Jul 2001 22:37:53 -0500
Subject: Ask-A-Microscopist: What is a flat field objective

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Below is the result of your feedback form. It was submitted by
(dkray-at-siu.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
July 19, 2001 at 14:53:44
---------------------------------------------------------------------------

Email: dkray-at-siu.edu
Name: Dave Krayesky

Organization: SIUC

Education: Graduate College

Location: Carbondale, IL 62901

Question: What is a flat field objective and how does it differ from
other types of objectives one might use on a microscope?

Thanks

Dave

---------------------------------------------------------------------------


From daemon Fri Jul 20 02:49:20 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Fri, 20 Jul 2001 08:40:34 +0100
Subject: copper retention-rubeanic acid

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Hello Listers

Can anyone comment on the retention of copper (in dosage experiments) by "staining" with rubeanic acid? What is the complex seen in light microscopy? Would it be accessible to x-ray microanalysis? ......... after removing a coverslip!

Keith Ryan
Marine Biological Association
Plymouth UK


From daemon Fri Jul 20 02:51:03 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Fri, 20 Jul 2001 08:47:51 +0100
Subject: copper retention-pyroantimonate?

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Hello Listers

Following my posting regarding copper/rubeanic acid, does anyone have information about the "pyroantimonate" method. A visitor to my lab has used it (at my suggestion) in an attempt to retain copper in accumulation/stress experiments from a home-base where cryomethods were not possible.

After a week of looking, we see very little in thin/semi-thin sections with XRMA. It is a method where chemical fixation is combined with potassium pyroantimonate to complex and retain the copper (and any other cations?.

Keith Ryan
Marine Biological Association
Plymouth, UK



From daemon Fri Jul 20 07:43:31 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Fri, 20 Jul 2001 22:30:48 +1000
Subject: Announcement - SEM and TEM Courses in Canberra October 2001

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PROTRAIN/ANU Courses 2001

Steve Chapman (Protrain Ltd) will be in Australia this October to give courses at the Electron Microscopy Unit of the Australian National University in Canberra. There will be a total of 8 days covering SEM and TEM, divided into 4 modules -you may enrol in one or more modules. Courses will be taught by Steve with assistance from ANU EMU staff.

Brief description below: details and enrolment on www.anu.edu.au/EMU/workshops/01chapman.html

I. SEM MASTERCLASS Tuesday 16th to Thursday 18th October. Three days: AUS$600

II. Maintaining and Monitoring the SEM. Friday 19th October. Pre-requisite: completion of this or a recent SEM Masterclass, or equivalent. One day: AUS $250

III. TEM Maintenance . Monday 22nd October, Tuesday 23rd October. Pre-requisite: familarity with TEM operation. Two days: AUS $500

IV. TEM Operation. Wednesday 24th October, Thursday 25th October. Two days: AUS $400

Meals and accommodation not included.

See you there - your opportunity to learn about EM instrumentation in a very practical way, and have a great time. This will be the third time we have run Module I - so far the recedivist rate is about 30%.

Sally








Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU





From daemon Fri Jul 20 07:48:36 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 20 Jul 2001 08:42:46 -0400
Subject: RE: Removing DPX/cover glass

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Cogent comment. If the coverglass was mounted with the minimum of
DPX, then the time required will be "over the weekend" or more. This from
long experience with removing coverglasses cracked by students and a common
excuse, "I thought it was parfocal!"
Cogent comment? If the lab is cold/cool, put it all in a screw cap
Coplin jar and set IT, not immersed, in a tepid water bath.

Question. I've never used DPX. Is it's solvent xylene? If it's
plastic and it dries to plastic, then you might have to use acetone to get
by the perimeter seal.

Irrational comment. OK, I won't remove all the chemicals from the
lab.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.



} ----------
} From: Keith Ryan
} Reply To: kpr-at-mba.ac.uk
} Sent: Thursday, July 19, 2001 9:37 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Removing DPX/cover glass
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Greetings from Plymouth, UK
}
} Can anyone advise on removing a coverslip mounted using DPX? I am
} currently soaking it in xylene, but overnight didn't "cut the mustard"!
} Any cogent comments? Please?!
}
} Keith Ryan
} Marine Biological Association
} Plymouth
}
}
}


From daemon Fri Jul 20 07:57:38 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 20 Jul 2001 08:52:01 -0400
Subject: SEM, Liquid Inclusions in Salt Crystals

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Good Morning Listers,

We are close to choosing a new SEM and have come to the conclusion
that if we want to view salt crystals with liquid inclusions we must do so
under positive chamber pressure in order to reduce explosive cleanups.
I have not dealt with such specimens before, so I am relying on
common sense when expressing this conclusion. The problem is that my
conclusion appears to reduce the market to one.
I would like to know if I have this right or not.

Thanks,

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
email: fmonson-at-wcupa.edu
Please call before visiting.


From daemon Fri Jul 20 09:45:28 2001



From: jshields-at-cb.uga.edu
Date: Fri, 20 Jul 2001 10:39:26 -0400
Subject: cryo unit available

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Hi All,
We have a BioRad/Polaron E-7000 cryo unit (that was attached to
a Philips SEM) available to a good home.
Please contact me offline.
Thanks
john shields
Univ. of Athens, GA USA
jshields-at-cb.uga.edu
(706)542-4080


From daemon Fri Jul 20 09:54:28 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 20 Jul 2001 09:50:14 -0500
Subject: Re: SEM, Liquid Inclusions in Salt Crystals

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I can't say that I have ever looked at such crystals. Are you hoping to
image the inclusions? I know we can see some subsurface voids at higher
voltages (20-25kV), but I think you would simply not see much.

How much pressure difference does it take to rupture the crystals? How much
internal pressure of the fluid inclusions? Remember that even under
absolute vacuum you have only raised the pressure differential by 15 psi. I
would think most materials could contain that. Even a 10 torr atmosphere
would not spare you much stress. I am not sure what you mean by a scope
with positive pressure.

I suppose that there may always be a few inclusions that would blow under
vacuum. I would think a pre-pump in a less expensive enclosure would cause
those to fail and thus spare the ESEM from harm.

Warren

At 08:52 AM 7/20/2001 -0400, you wrote:
} Good Morning Listers,
}
} We are close to choosing a new SEM and have come to the conclusion
} that if we want to view salt crystals with liquid inclusions we must do so
} under positive chamber pressure in order to reduce explosive cleanups.
} I have not dealt with such specimens before, so I am relying on
} common sense when expressing this conclusion. The problem is that my
} conclusion appears to reduce the market to one.
} I would like to know if I have this right or not.
} Thanks,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)



----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Jul 20 09:58:50 2001



From: Tom Malis :      malis-at-nrcan.gc.ca
Date: Fri, 20 Jul 2001 10:54:34 -0400
Subject: Re: Sectioning a crystalline powder sphere

Contents Retrieved from Microscopy Listserver Archives
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I'm assuming (though you don't actually say) that it is thin sections that
you are after. If so, I'm not surprised that embedding and sectioning was
unsuccessful. You have one of those challenging specimens that profs seem
to love handing to grad students, either with the expectation that specimen
prep is trivial, or, knowing it is a beast, seeing if the grad student can
come up with a new wrinkle!

You don't actually say that it is absolutely necessary for the analysis that
the section hold together. Could you do a thin section, say, 100-200nm, do
an area analysis of the (dry-sectioned) pulverized bits (lying on a coated
grid on the diamond near the knife edge), then trim very coarse for, say 50
microns, then thin section again, and so on? If there is a 30 degree
diamond knife around, you might reduce the 'pulverizing' action.

Alternatively, find someone in the neighbourhood with a focused ion beam
(FIB) system, usually in Physics or Engineering. We have access to one and
have sectioned (though for SEM analysis so we didn't try thin sectioning)
some pretty delicate hydroxide powders with no mechanical damage
whatsoever. If your particle is 'reinforced' with resin, a FIB-cut section
should still be intact after it is cut through and flops on its side, since
the incident Ga ion beam is at near zero degrees. The tricky part will come
in getting the section onto a grid with something called a micromanipulator
(a very fine glass fibre - electrostatic attraction makes the section 'fly
up' onto the tip, hopefully intact, then you move the section over the grid
and gently press it down). Most FIB types I know would be intrigued by the
demanding nature of your specimen.

Good luck!

Tom Malis
malis-at-nrcan.gc.ca

} From: Margaret Miller {MILLERMM-at-uthscsa.edu}
} Date: Thu, 19 Jul 2001 22:18:21 -0500
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Sectioning a crystalline powder sphere
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} --
} To Listers,
}
} I am a graduate student at the university of Texas Health Science Center.
} As part of a project I wish to perform a spectral analysis of a
} certain medication. The medication is a crystalline powder in the
} form of small (approx. 1mm diameter) spheres. I want to section the
} spheres to 10 to 30 microns so as to obtain a spectral analysis of
} the entire bead from its outer coating to its interior. I have tried
} OCT embedding and cyrostat sectioning. However, the beads pulverized
} during sectioning. Can anyone recommend a way to section these
} spheres so as to preserve the material as much as possible? I should
} also point out that the material is freely soluble in ethanol and
} methanol, as well as water.
}
} Thank you in advance for your help and suggestions.



From daemon Fri Jul 20 10:49:17 2001



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Fri, 20 Jul 2001 17:42:03 +0200
Subject: Re: DTSA

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Dear all,
I have found out the source of my particular problem with importing files
into DTSA. It was caused by the way I transferred them over the network to
the Mac with Fetch. It did not recognise the files as text files and
therefore did not treat them as such. When I explicitly told Fetch that
they were Text files, however, they could be opened in DTSA.

So, my problem was not caused by different variations in EMSA file formats,
but by the fact that the Mac didn't know that they were text files.

Thanks again for all the suggestions, I seem to have set a huge discussion
raging by a seemingly innocent comment about difficulties importing my
files!

Best wishes to all.

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/



From daemon Fri Jul 20 12:16:31 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 20 Jul 2001 09:25:07 -0700
Subject: Re: microscopy manual

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} We have inherited a Leitz Metallux 3 microscope with epifluorescence,
} however, without a user's manual (not a complaint, just a fact) and are
} wondering whether anyone knows a source for old microscope manuals.

Judy -

There's a microscopy book dealer in the UK; try them:
http://www.savonabooks.free-online.co.uk

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Jul 20 12:22:14 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 20 Jul 2001 12:15:27 -0500
Subject: Re: Sectioning a crystalline powder sphere

Contents Retrieved from Microscopy Listserver Archives
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Being an SEM guy, I wonder about embedding it and dry sanding it to a cross
section with SiC paper. Water is normally used for a lubricant, but you
could forego that and just use a few more sheets of paper.

Such a sample would not be good for TEM, but I would think an SEM could
handle it well enough.

Warren

At 10:18 PM 7/19/2001 -0500, you wrote:
} --
} To Listers,
}
} I am a graduate student at the university of Texas Health Science Center.
} As part of a project I wish to perform a spectral analysis of a certain
} medication. The medication is a crystalline powder in the form of small
} (approx. 1mm diameter) spheres. I want to section the spheres to 10 to 30
} microns so as to obtain a spectral analysis of the entire bead from its
} outer coating to its interior. I have tried OCT embedding and cyrostat
} sectioning. However, the beads pulverized during sectioning. Can anyone
} recommend a way to section these spheres so as to preserve the material as
} much as possible? I should also point out that the material is freely
} soluble in ethanol and methanol, as well as water.
}
} Thank you in advance for your help and suggestions.

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Jul 20 14:29:28 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 20 Jul 2001 14:22:51 -0500
Subject: Margaret Miller/ sections of pills

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marbaret:
I would be tempted to use a metallographic approach (being a
metallographer). Go over to the Materials Science guys for guidance in
making an epoxy mount. Talk to Dr. Mark Cavaleri of MMM (mecavaleri-at-mmm.com)
about polishing methods. He did a complete section of an M&M without losing
any of the sugars.

Good luck,

Sam Purdy
Teech. Center
National Steel Corp.
Trenton MI 48183
spurdy-at-nationalsteel.com


From daemon Fri Jul 20 14:40:09 2001



From: EvanPaassen ()
Date: Fri, 20 Jul 2001 14:36:58 -0500
Subject: Ask-A-Microscopist: used binocular stereo microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form. It was submitted by
(EvanPaassen) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, July
20, 2001 at 14:32:10
---------------------------------------------------------------------------

Email: EvanPaassen
Name: Ed van Paassen

Organization: WAU

Education: Graduate College

Location: City, State, Country

Question: Does anyone know where I can get a used binocular stereo microscope?


---------------------------------------------------------------------------


From daemon Sat Jul 21 20:34:40 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Sat, 21 Jul 2001 18:16:38 -0700 (PDT)
Subject: RE: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dennis:
Altho' I have to use Access from time to time, it is not the program with
which I am most familiar. Several years ago (over 10, believe it or not) I
used Paradox to develop a database for the lab work going on--both EM and
LM. It has been invaluable, and I have been able to maintain things through
upgrades (altho' I think I may have missed the next to last one!). There
has never been a problem transferring my data from one version to the next.
I personally find Paradox easier to work with than Access, but then I
started out using WordPerfect, and I still prefer that program. Another
(and, _Free_) program is the 602ProSuite of products. I have only used the
wordprocessing package so far, but it seems to have most of what I need/use.
The program has received great reviews on the ZDNet website. And it's a
fantastic price. Back to Paradox--one of the things I like best is the
ability to go back and modify your database without losing (or just as bad,
having to reenter everything) data. As projects/experiments have
expanded/changed or been linked to other studies (via technique, reagents,
etc) new categories can be added. Likewise, the search functions permit
cross-database searches for seemingly (or unsuspected) relationships.

Roger

No financial interest--just a very satisfied, long-term user.
On Thu, 19 Jul 2001 14:37:41 -0400, Dennis C. Winkler wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Greg and others,
|
| Clarification: Yes, I am talking about physical micrographs
| (film/negatives). Sorry about the confusion.
|
| Thanks for all of the suggestions thus far. My first thought was to
| set up a FileMakerPro database to store the information, but I will
| look at the commercial packages that have been mentioned.
|
| - Dennis
|
| ------------------------------------------------------------------------
| Dennis C. Winkler, PhD. Phone: (301) 496-0131
| Laboratory of Structural Biology Research Fax: (301) 480-7629
| NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
| Bldg. 6, Room B2-26, MSC-2717
| Bethesda, MD 20892-2717, U.S.A.
|
|
| At 9:27 AM -0400 7/19/01, Greg Erdos wrote:
| } ------------------------------------------------------------------------
| } The Microscopy ListServer -- Sponsor: The Microscopy Society of
| } America To Subscribe/Unsubscribe -- Send Email to
| } ListServer-at-MSA.Microscopy.Com
| } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| } -----------------------------------------------------------------------.
| }
| }
| } ThumbsPlus is good if you have a digital image to keep track of, but
| } I am not sure if Dennis was referring to that or to physical
| } micrographs. If it is the latter then I would suggest using
| } Microsoft Access and setting up their own forms and reports to suit
| } their particular needs.
| } Greg Erdos
| }
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Sun Jul 22 12:57:24 2001



From: Edwards Forensic Service :      fedwards-at-mninter.net
Date: Sun, 22 Jul 2001 12:51:47 -0600
Subject: Re: Ask-A-Microscopist: used binocular stereo microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In answer to Ed van Paassen's inquiry: I have two used microscopes for
sale:

1. Bausch & Lomb stereo binocular zoom microscope with illuminator.
2. Meiji polarizing light microscope with trinocular head.

If interested please contact me directly at fedwards-at-mninter.net

Harold Edwards




From daemon Sun Jul 22 13:27:19 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 22 Jul 2001 13:21:51 -0500
Subject: M&M 2001

Contents Retrieved from Microscopy Listserver Archives
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To all,
The annual meetings are quickly approaching. We will again hold a session with discussion centering around topics pertaining to Core Facility Management. The topics and session facilitators are listed at the end of this message. Using the same format as last year, the facilitators will give introductions to the topics and then there will be extensive discussion involving all attending.

The session is scheduled for 8:00AM Monday August 5 in Room 201A. I hope to see lots of early risers as, due to later scheduling which partially conflicts with this session, we will have to start on time and move right along to cover the topics.

Please feel free to bring handouts or overhead if you feel they might be useful in the discussions. Course outlines might be very helpful for the first topic. Illustrations to help clarify questions pertaining to scientific ethics in the performance of our duties might also be helpful for the last topic.

Looking forward to seeing old friends and meeting new fellow microscopists at the meeting.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907
============================================================================

M&M 2001
Core Facility Management “Experts” session


This session is intended to highlight topics that are of concern or interest to managers of microscopy facilities in both academic and industrial settings and with emphasis in life sciences and/or materials areas. There will be three topics discussed at M&M 2001. Each topic will be introduced by one or more facilitators. The introduction will be followed by an extended open discussion with the audience.

Proceedings will be recorded and transcribed so they can be made available to interested persons after the meeting.


1) Training Users: Courses? Workshops? … what works for whom?

Facilitator: John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
Southern Illinois University



2) Calibration of Electron Microscopes: how to do this, how often, pit-falls and problems.

Facilitators:
SEM: Michael Postek,
Group Leader, Nanometer-scale Metrology
National Institute of standards and Technology (NIST)

TEM: John P. McCaffrey
National Research Council of Canada


3) Scientific Ethics: what are our responsibilities as facility managers?

Facilitator: Michael Kalichman
Director, Research Ethics Program
Office of Graduate Studies and Research, 0003
University of California, San Diego








From daemon Sun Jul 22 17:44:18 2001



From: Bob Roberts :      bobrobs-at-earthlink.net
Date: Sun, 22 Jul 2001 15:41:11 -0700
Subject: SEM EDS Mounting Flange

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Message-ID: {3B5B5687.3060704-at-earthlink.net}


Listers,

I am looking for an EDS detector mounting flange for a JEOL
JSM-IC845 SEM. The detector to be mounted is a Tracor
Northern, with a 40 deg take off angle.

If someone has one of these and would like to part with it,
please contact me off line. Thanks.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
480.967.3946



From daemon Mon Jul 23 08:21:01 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Jul 2001 08:06:48 -0500
Subject: M&M 2001

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Further clarification.....the date of the session is Monday, August 6 not Monday August 5 as indicated below. Secondly all topics will be covered in order listed below in room 201A. Each topic is assigned 45 min for facilitator's introduction followed by open discussion. I know this may not be sufficient for everyone to speak their piece but it is all the time we were allotted. By keeping on track we can still cover a lot of ground. Perhaps those topics that need more time can be dealt with in more detail at future meetings.
Debby



To all,
The annual meetings are quickly approaching. We will again hold a session with discussion centering around topics pertaining to Core Facility Management. The topics and session facilitators are listed at the end of this message. Using the same format as last year, the facilitators will give introductions to the topics and then there will be extensive discussion involving all attending.

The session is scheduled for 8:00AM Monday August 5 in Room 201A. I hope to see lots of early risers as, due to later scheduling which partially conflicts with this session, we will have to start on time and move right along to cover the topics.

Please feel free to bring handouts or overhead if you feel they might be useful in the discussions. Course outlines might be very helpful for the first topic. Illustrations to help clarify questions pertaining to scientific ethics in the performance of our duties might also be helpful for the last topic.

Looking forward to seeing old friends and meeting new fellow microscopists at the meeting.
Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907
============================================================================

M&M 2001
Core Facility Management “Experts” session


This session is intended to highlight topics that are of concern or interest to managers of microscopy facilities in both academic and industrial settings and with emphasis in life sciences and/or materials areas. There will be three topics discussed at M&M 2001. Each topic will be introduced by one or more facilitators. The introduction will be followed by an extended open discussion with the audience.

Proceedings will be recorded and transcribed so they can be made available to interested persons after the meeting.


1) Training Users: Courses? Workshops? … what works for whom?

Facilitator: John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
Southern Illinois University



2) Calibration of Electron Microscopes: how to do this, how often, pit-falls and problems.

Facilitators:
SEM: Michael Postek,
Group Leader, Nanometer-scale Metrology
National Institute of standards and Technology (NIST)

TEM: John P. McCaffrey
National Research Council of Canada


3) Scientific Ethics: what are our responsibilities as facility managers?

Facilitator: Michael Kalichman
Director, Research Ethics Program
Office of Graduate Studies and Research, 0003
University of California, San Diego








From daemon Mon Jul 23 08:21:01 2001



From: jshields-at-cb.uga.edu
Date: Mon, 23 Jul 2001 09:07:28 -0400
Subject: revised - cryo unit

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To: Microscopy-at-sparc5.microscopy.com


To clarify the previous listing,

The BioRad/Polaron cryo unit was originally attached to a Philips
505 and has been in storage for quite some time. I was told it had
problems prior to being boxed up.
It will be better suited as spare parts for anyone with an existing
unit.
Sorry for the previous incompleteness.
John Shields
EM Lab
Univ. of Georgia
Athens, GA 30602

jshields-at-cb.uga.edu


From daemon Mon Jul 23 09:19:18 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 23 Jul 2001 07:13:05 -0700
Subject: Zeiss -- what's going on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm not looking to start a flame war here. Please
bear with me.

I was going to update my Zeiss Axioplan DIC/phase
system to a newer model. I got some major reverberations
and feedback that something very bad has happened
regarding Zeiss the company. Most feedback seems to
be along the lines of bad customer service, no parts,
and no support.

My Zeiss systems have been working great for several
years and I have not needed any factory support. I ordered
some fine clock oil for a condenser and a few other
odd and ends and had no problems. My Plan Neofluars
don't wear out. So what is the big deal with Zeiss Co. these
days?

I have no financial interest in Zeiss. I only have a financial
interest in not getting burned. To avoid public acrimony,
perhaps private responses off-line are appropriate. I would
certainly appreciate some info about what I am obviously
missing.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
7970 Twin Rocks Rd
Granite Bay, CA 95746

916.791.8191



From daemon Mon Jul 23 09:26:20 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Mon, 23 Jul 2001 09:14:57 -0500
Subject: Re: M&M 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Debby Sherman:

Would you confirm us the Core Facility Management session is scheduled for Monday August 6 not Sunday August 5. In case of Aug 5, we have to change our schedule for the flight and hotel.

Thanks

Greg Ning
EM Facility
Medical College of Wisconsin

Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} To all,
} The annual meetings are quickly approaching. We will again hold a session with discussion centering around topics pertaining to Core Facility Management. The topics and session facilitators are listed at the end of this message. Using the same format as last year, the facilitators will give introductions to the topics and then there will be extensive discussion involving all attending.
}
} The session is scheduled for 8:00AM Monday August 5 in Room 201A. I hope to see lots of early risers as, due to later scheduling which partially conflicts with this session, we will have to start on time and move right along to cover the topics.
}
} Please feel free to bring handouts or overhead if you feel they might be useful in the discussions. Course outlines might be very helpful for the first topic. Illustrations to help clarify questions pertaining to scientific ethics in the performance of our duties might also be helpful for the last topic.
}
} Looking forward to seeing old friends and meeting new fellow microscopists at the meeting.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-099e Whistler Building
} West Lafayette, IN 47907
} ============================================================================
}
} M&M 2001
} Core Facility Management “Experts” session
}
} This session is intended to highlight topics that are of concern or interest to managers of microscopy facilities in both academic and industrial settings and with emphasis in life sciences and/or materials areas. There will be three topics discussed at M&M 2001. Each topic will be introduced by one or more facilitators. The introduction will be followed by an extended open discussion with the audience.
}
} Proceedings will be recorded and transcribed so they can be made available to interested persons after the meeting.
}
} 1) Training Users: Courses? Workshops? … what works for whom?
}
} Facilitator: John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} Southern Illinois University
}
} 2) Calibration of Electron Microscopes: how to do this, how often, pit-falls and problems.
}
} Facilitators:
} SEM: Michael Postek,
} Group Leader, Nanometer-scale Metrology
} National Institute of standards and Technology (NIST)
}
} TEM: John P. McCaffrey
} National Research Council of Canada
}
}
} 3) Scientific Ethics: what are our responsibilities as facility managers?
}
} Facilitator: Michael Kalichman
} Director, Research Ethics Program
} Office of Graduate Studies and Research, 0003
} University of California, San Diego
}
}
}



From daemon Mon Jul 23 10:13:24 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 23 Jul 2001 11:06:42 -0400
Subject: RE: SEM, Liquid Inclusions in Salt Crystals, Re: Crow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Fred,
Please refer to the American Vacuum Society site:
http://www.aip.org/avsguide/refguide/pressure.html for a fast and useful
education in the meaning of pressure. Through the kindness, and
forbearance, of Bill Miller you have been saved from exhibiting your own
version of ignorance any further.
You should apologize to all the non-vacationing listers for exposing
yourself in this manner. In future, some information on the basics would
probably save you from this kind of embarrassment.
First of all, I suspect you would have been helped if the phrase
"variable pressure" had been correctly(?) (mis?)stated as "variable
vacuum".[there's that period again!] As Bill said, even the best SEM's
cannot operate with the chamber open, and you should, with all of your
'smarts', have been able to see that. Once you have your brain switched to
the proper channel, I am certain you will see this point and stop bothering
these people.
Finally, and I'm sure I speak for all, you should take your own
advice and look in the books first. But also, please remember what I always
tell my students. The only dumb question is the one you don't ask. Well,
as you can see, that's not quite the truth, but it's pretty good advice.
And, finally, finally, about your question. Perhaps what you should
have asked was how users of SEM technology look at water in the liquid
state?

Regards and apologies,

Fred Monson


} ----------
} From: Monson, Frederick C.
} Sent: Friday, July 20, 2001 8:52 AM
} To: 'Microscopy Listserver'
} Subject: SEM, Liquid Inclusions in Salt Crystals
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good Morning Listers,
}
} We are close to choosing a new SEM and have come to the conclusion
} that if we want to view salt crystals with liquid inclusions we must do so
} under positive chamber pressure in order to reduce explosive cleanups.
} I have not dealt with such specimens before, so I am relying on
} common sense when expressing this conclusion. The problem is that my
} conclusion appears to reduce the market to one.
} I would like to know if I have this right or not.
}
} Thanks,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} West Chester University of Pennsylvania
} Center for Advanced Scientific Imaging (CASI)
} Schmucker II Science Center (Room: SS024(Basement))
} South Church Street
} West Chester, PA, 19383
} MailDrop: Department of Geology/Astronomy
} Phone: 610-738-0437
} Fax: 610-436-3036
} email: fmonson-at-wcupa.edu
} Please call before visiting.
}
}


From daemon Mon Jul 23 12:12:48 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 23 Jul 2001 13:05:09 -0400
Subject: RE: copper retention-rubeanic acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evening Keith,
Check Wilson's disease.
rubeanic acid chelates the Cu, Co or Ni
Check Merck Index
Ray, Xavier(1961), J Indian Chem Soc, 38:535(review)
Have no experiece with EM for this problem.
Also:
http://www.ninds.nih.gov/health_and_medical/disorders/wilsons_doc.htm

Regards and let us know how the marine bio studies go.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
Schedule: http://bio.wcupa.edu/cgi-bin/webreserve.acgi
Please call before visiting.


} ----------
} From: Keith Ryan
} Reply To: kpr-at-mba.ac.uk
} Sent: Friday, July 20, 2001 3:40 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: copper retention-rubeanic acid
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello Listers
}
} Can anyone comment on the retention of copper (in dosage experiments) by
} "staining" with rubeanic acid? What is the complex seen in light
} microscopy? Would it be accessible to x-ray microanalysis? .........
} after removing a coverslip!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK
}
}


From daemon Mon Jul 23 12:31:03 2001



From: kszaruba1-at-mmm.com
Date: Mon, 23 Jul 2001 12:26:01 -0500
Subject: RE: Database of Archived EM Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dennis,

I did set up a Filemaker Pro database years ago to log TEM micrographs as
I was shooting them. I used the database to make labels for the
micrographs (big time-saver for me), print a list of negatives shot at
each session and do annual reviews of project work. It was also useful as
a searching tool, for instance if I forgot the date that I last examined
samples from a certain project. It would be easy to set up a similar
database with a field for micrograph storage information.

Note: the initial plan was to set up the database for a whole microscopy
lab of mixed PC and Mac-users, but we had filesharing problems over our
network system. I did think Filemaker Pro was easier to use than Access
(which I converted to after everything went PC). Don't get me wrong, I
like using Access as well. But for creating a simple text-based database
Filemaker was very straightforward, even if it had some limitations
regarding relational comparisons (it's been so long I can't remember what
the limitations were, just that they existed!).

I should emphasize that what made the system most convenient for me was
having it set up on the computer attached to the TEM. That way I could
enter each record as soon as the negative was exposed, the same way one
would make an entry in a logbook. It took a few seconds longer than
handwriting, but overall it did not feel like an extra chore to do the
data entry.

Caveat: I set this up about 8 years ago, and haven't worked with
Filemaker Pro for over 3 years. Things may have changed... But if you
are interested I'd be glad to send a copy of the database template (if I
can still find it).
Hope it works out for you,
Karen
--------------------------------------------------------------------------------
Karen S. Zaruba
BioMaterials Technology Center
3M Center Bldg. 270-1S-01
St. Paul, MN 55144
kszaruba1-at-mmm.com





"Dennis C. Winkler" {Dennis_Winkler-at-nih.gov}
07/19/01 01:37 PM


To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: Karen S. Zaruba/US-Corporate/3M/US)
Subject: RE: Database of Archived EM Micrographs



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Greg and others,

Clarification: Yes, I am talking about physical micrographs
(film/negatives). Sorry about the confusion.

Thanks for all of the suggestions thus far. My first thought was to
set up a FileMakerPro database to store the information, but I will
look at the commercial packages that have been mentioned.

- Dennis

------------------------------------------------------------------------
Dennis C. Winkler, PhD. Phone: (301) 496-0131
Laboratory of Structural Biology Research Fax: (301) 480-7629
NIAMS, National Institutes of Health Email: Dennis_Winkler-at-nih.gov
Bldg. 6, Room B2-26, MSC-2717
Bethesda, MD 20892-2717, U.S.A.







From daemon Mon Jul 23 18:30:29 2001



From: srinivas.mullapudi-at-uth.tmc.edu ()
Date: Tue, 24 Jul 2001 12:53:17 -0500
Subject: Ask-A-Microscopist: Focus definitions needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To:
microscopy-at-msa.microscopy.com,CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU


Zeiss has always been a company that has supported their products dating
back to almost the stone age. It seems that has come to an end. They broke
(terminated) their agreements with their whole dealer base, the independent
companies who had been supporting Zeiss products for decades. Now they are
not supporting microscopes the have just sold a year or two ago. We have a
brand new Zeiss microscope in our Emergency Room Lab that has a bad lens.
No parts available to repair it!!! I'm supposed to throw away a new $5000
dual head microscope because the company won't support it?

I no longer recommend purchasing Zeiss products.

There have always been other companies who offer much better value then
Zeiss. The redeeming value of Zeiss has been in the past that they offered
almost unlimited support for their older products. No more.

Dave Burton
Optical Specialist




----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, July 23, 2001 7:13 AM


Correction - Rather than being included as attachments to the previous
message, contents of the Late Breaking Poster Session and the
Corporate Members Platform Session have been added to the meeting
web site. You can access these by clicking the Symposia icon on the
Microscopy and Microanalysis 2001 web site.


------- Forwarded Message Follows -------
Date sent: Mon, 23 Jul 2001 18:22:05 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: "Bob Price" {price-at-dcsmserver.med.sc.edu}


Below is the result of your feedback form. It was submitted by
(srinivas.mullapudi-at-uth.tmc.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
July 24, 2001 at 10:07:20
---------------------------------------------------------------------------

Email: srinivas.mullapudi-at-uth.tmc.edu
Name: Mullapudi Srinivas

Organization: University of texas medical School

Education: Graduate College

Location: Houston tx USA

Question: I want to know the exact meaning of terms
defocus,underfocus,focus,infocus and overfocus how
they are classified for a particular object.

I would appreciate receiving reply in this regard



---------------------------------------------------------------------------


From daemon Tue Jul 24 14:38:21 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Tue, 24 Jul 2001 15:49:59 -0400
Subject: compound for immobilization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists :

We are attempting to visualize pulmonary vasculature. One method would be to perfuse an animal with a fluorescent dye - for example FITC-dextran - but, since we expect to section the lung, the dye would leak out at the severed edges.

Does anyone know of a compound in which to disolve the dye and perfuse the animal with, that would polymerize into a soft solid and remain within the vessels? I remember reading about something like this used to immobilize suspension cells in a culture dish.

Thank you





Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Tue Jul 24 15:07:53 2001



From: Eller, Rick :      ellerro-at-tsmd.com
Date: Tue, 24 Jul 2001 16:02:30 -0400
Subject: Grain Size Measurement of Thin Metal Films ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to measure the grain size of 1 micron thick Al0.5%Cu films on Single
Crystal Silicon substrate.
These are deposited by magnetron sputtering process. Any suggestions or
experience out there?
Thanks
Rick Eller



From daemon Tue Jul 24 21:48:43 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Tue, 24 Jul 2001 21:36:49 -0500
Subject: Kodak mds 100 camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What kind of results have some of you had with Kodak MDS 100? The price is
getting pretty reasonable for the resolution if the software works well
enough to be usable. My particular concern it lack of manual control over
exposure.

TIA
Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger



From daemon Tue Jul 24 21:58:19 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 24 Jul 2001 22:54:22 -0500
Subject: Corrision casting of pulmonary vasculature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Judy Trogadis wrote:
==============================================================
We are attempting to visualize pulmonary vasculature. One method would be to
perfuse an animal with a fluorescent dye - for example FITC-dextran - but,
since we expect to section the lung, the dye would leak out at the severed
edges.

Does anyone know of a compound in which to disolve the dye and perfuse the
animal with, that would polymerize into a soft solid and remain within the
vessels? I remember reading about something like this used to immobilize
suspension cells in a culture dish.

==============================================================
I believe the "compound" you are referring to is the Mercox™ Corrosion
Casting System, it comes in two different colors (red and green) plus clear
(for SEM applications). You can see information about it on our website at
URL
http://www.2spi.com/catalog/chem/mercox-corrosion-casting.html

You can also see reproduced on the website an article that appeared in
Microscopy Today, "Vascular Corrosion Casting Can Provide Quantitative as
Well as Morphological Information on the Microvasculature of
Organs and Tissues" by Fred E. Hossler, J. H. Quillen College of Medicine,
Issue 98-7, Sept. 1998, or see URL
http://www.2spi.com/catalog/chem/mercox-casting.html

Disclaimer: SPI Supplies distributes the Mercox kits to researchers
worldwide.

Chuck


===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Tue Jul 24 23:51:52 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Tue, 24 Jul 2001 23:45:23 -0500
Subject: Re: Kodak mds 100 camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



What kind of results have some of you had with Kodak MDS 100?
The price is getting pretty reasonable for the resolution if the software
works well enough to be usable. My particular concern it lack of manual
control over exposure.

Thanks
Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger




From daemon Wed Jul 25 00:25:36 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Wed, 25 Jul 2001 17:15:30 +1200
Subject: Nikon Epi-Fluorescence Attachment for Stereo Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone any experience with the Nikon Epi-Fluorescence
Attachment for a Stereo Microscope. For example how does it
compare with the Leica MZ FLIII Fluorescence Stereo
Microscope?? Any personal responce will be treated in confidence.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Wed Jul 25 05:34:08 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 25 Jul 2001 06:26:38 -0400
Subject: New Ladd contact info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For all those who did not receive our mailing, our new address is:

Ladd Research
83 Holly Court
Williston, VT 05495

New Telephone # - 802-658-4961
New Fax # - 802-660-8859

Thank you,

John Arnott
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


From daemon Wed Jul 25 06:09:37 2001



From: Richard Gardiner :      rbgardiner-at-home.com
Date: Wed, 25 Jul 2001 07:06:07 -0400
Subject: Re: Lietz Microscope Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone have a copy of a Leitz manual for a Dailux 20 with the
epifluorescence head? We have one that we want to use and would like
information on the filter sets etc..

Thanks Richard Gardiner



From daemon Wed Jul 25 08:18:24 2001



From: Don.Steele-at-alcan.com
Date: Wed, 25 Jul 2001 09:11:29 -0400
Subject: Re: Grain Size Measurement of Thin Metal Films ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The first method that comes to mind, is to ion mill from the Si side, and
then look in the TEM. I've done this in pre PIPS days on dimpled samples.

I also recall rigging up a chemical method where the Si was etched away (a
mixture of nitric acid and HF. I can't recall the concentrations, but it
was likely something standard which others might still be using) leaving
the surface film, again for TEM.

If you are handy with a microtome, surface slices coud be taken from a bit
of the wafer glued to a dummy block. This will give sections thin enough
for TEM, but there will be compression artifacts.

And the last "top of my head" suggestion... at 1 micron thick, are the
grains large enough to see using channeling contrast in the SEM?



"Eller, Rick"
{ellerro-at-tsmd To: "'Microscopy-at-MSA.Microscopy.Com'"
.com} {Microscopy-at-sparc5.microscopy.com}
cc:
07/24/01 Subject: Grain Size Measurement of Thin Metal Films ?
04:02 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I wish to measure the grain size of 1 micron thick Al0.5%Cu films on Single
Crystal Silicon substrate.
These are deposited by magnetron sputtering process. Any suggestions or
experience out there?
Thanks
Rick Eller







From daemon Wed Jul 25 08:21:28 2001



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Wed, 25 Jul 2001 09:16:58 -0400
Subject: ESEM/Light Microscopy high temperature environmental chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists:
We are looking for an environmental chamber that will allow us to heat
materials to approximately 1100 C under different reactive gasses while
viewing in-situ by either SEM (ESEM) or light microscopy. ESEM would be
preferred due to the size range of the subject. We would be interested in
contracting this work to your lab, "renting" the chamber or purchasing
outright, depending on what's available.
Please contact us directly with responses so we don't clutter the list!!
Thanks in advance.
Bill
William A. Heeschen
Microscopy, Digital Imaging
The Dow Chemical Company
1897 Bldg.
Midland, MI 48667-1897
waheeschen-at-dow.com
voice: 989-636-4005 fax: 989-638-6443



From daemon Wed Jul 25 09:33:14 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 25 Jul 2001 10:25:30 -0400
Subject: RE: compound for immobilization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Judy,

Actually, one merely has to inject Evans Blue into the experimental
animals vasculature, and the albumen in the blood will be instantly bonded
by the dye. The dye is supposed to fluoresce only when so bonded. The
other way, of course is to set up the albumen-Evans Blue first (see
Kodani(?) in '60's for testicular barrier studies)followed by suitable
dialysis of unbound EVB (stoichiometry is apparently 1:1). Fixation
followed by sectioning with fluorescence. FITC-?, though bound with greater
avidity than plain fluorescein does not elicit the dye fastness of EVB with
albuminoids.

Evans Blue Dye Site (Blood volume): http://www.evansblue.co.uk/index.html

Caveat:
http://web.missouri.edu/~pharmrl/abstracts/jackiewicz_micro_1998.htm

Method:
http://www.merck.de/english/services/labor/cell/adye/applidye3169.htm

This is everything but the trip to the confocal (see Czymmek, K (2000),
"Imaging and volumetric quantitation of vascular corrosion casts with laser
scanning confocal microscopy, Proceedings: Microscopy and Microanalysis
2000, Philadelphia, PA, August 13-17, 2000, p 562.
(kirk.czymmek-at-mvs.udel.edu). Using Mercox Blue corrosion cast, excite 633nm
laser, ~660nm barrier.

Fred

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
Schedule: http://bio.wcupa.edu/cgi-bin/webreserve.acgi
Please call before visiting.


} ----------
} From: Judy Trogadis
} Sent: Tuesday, July 24, 2001 3:49 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: compound for immobilization
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Fellow Microscopists :
}
} We are attempting to visualize pulmonary vasculature. One method would be
} to perfuse an animal with a fluorescent dye - for example FITC-dextran -
} but, since we expect to section the lung, the dye would leak out at the
} severed edges.
}
} Does anyone know of a compound in which to disolve the dye and perfuse the
} animal with, that would polymerize into a soft solid and remain within the
} vessels? I remember reading about something like this used to immobilize
} suspension cells in a culture dish.
}
} Thank you
}
}
}
}
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Q
} 30 Bond St.
} Toronto, ON M5B 1W8
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
}
}


From daemon Wed Jul 25 13:47:53 2001



From: Smartech :      smartech-at-optonline.net
Date: Wed, 25 Jul 2001 14:50:34 -0400
Subject: SEM of super smooth material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want so show that a polymer film has not been etched after exposure to an
HCL based environment. The problem is that it is extremely smooth to begin
with. I would like to add some sub-micro features as a background so that I
am not generating a featureless gray photomicrograph. These features would
be essentially a topographic reference. I though of a mono layer of
particles dusted on the surface. I stated w/ common baking flour. The
particles were too large and not stuck to the surface so well (lots of edge
effect) so that one can't effectively have a good contrast of the spheres
and the background in the same micrograph. A very fine grid would also be
nice, but perhaps expensive. Smaller and flatter particles that were put in
solvent and allowed to dried might do the trick. Anybody have any
interesting suggestions or materials? I would be glad to share the results.

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Jul 25 14:35:12 2001



From: Meghan Towner :      town3648-at-uidaho.edu
Date: Wed, 25 Jul 2001 12:28:36 -0700 (PDT)
Subject: labeling with antibodies--TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Afternoon!
This is a follow up question involving tissue sections prepared for the TEM. My original question dealt with perfusion with physiological saline followed by cold 4% paraformaldhyde. I will be incubating the tissue (dorsal root ganglions) with an antibody. I have spoken to my advisor and Franklin Bailey at the UI EM center about preparing the tissue sections and eventual incubation with antibodies. I have received conflicting advice and am putting this out for more advice. Since I am going to use antibodies, does this change how I perfuse the animal? When incubating with the antibodies, should I do it pre- or postembedding? Immunogold labeling may be used and I have been told that preembedded labeling may damage the knife. I am open to other ways of labeling this tissue. Thank you.

Meghan Towner
Graduate Student
Biological Sciences
University of Idaho



From daemon Wed Jul 25 16:04:49 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 25 Jul 2001 16:33:31 -0400
Subject: SEM of super smooth material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try smoking some Mo with heat to produce a white smoke and wave your sample in the smoke. You will pick up small particle of MoO3. Alternatively, you could burn Mg and wave your sample in the smoke. Experiment with how high in the smoke you wave your sample.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
Sent: Wednesday, July 25, 2001 2:51 PM
To: To all on the list


I want so show that a polymer film has not been etched after exposure to an
HCL based environment. The problem is that it is extremely smooth to begin
with. I would like to add some sub-micro features as a background so that I
am not generating a featureless gray photomicrograph. These features would
be essentially a topographic reference. I though of a mono layer of
particles dusted on the surface. I stated w/ common baking flour. The
particles were too large and not stuck to the surface so well (lots of edge
effect) so that one can't effectively have a good contrast of the spheres
and the background in the same micrograph. A very fine grid would also be
nice, but perhaps expensive. Smaller and flatter particles that were put in
solvent and allowed to dried might do the trick. Anybody have any
interesting suggestions or materials? I would be glad to share the results.

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Jul 25 16:33:15 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Wed, 25 Jul 2001 17:31:41 -0400
Subject: Inspection/assembly microscope recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our company needs to get a microscope for inspection and
assembly/disassembly tasks. We would like it to have the following
characteristics:

1) Stereo viewing
2) Wide field of view
3) Prefer zoom capabilities, but could take anything that would allow
magnifications from about 4X to, say, 20X
4) Good working distance

Would anyone care to make any recommendations or do you have any suggestions
for us?

Vendor input is welcome. Contact information follows my sig.

Bruce Girrell
Microline Technology Corp.
2397 Traversefield Dr.
Traverse City, MI 49686
http://www.microlinetc.com

(231) 935-1585 (Voice)
(231) 922-5099 (FAX)
bigirrell-at-microlinetc.com



From daemon Wed Jul 25 18:30:36 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 25 Jul 2001 19:23:58 -0500
Subject: Etched polymer films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

SMARTech asked the following:
===========================================================
I want so show that a polymer film has not been etched after exposure to an
HCL based environment. The problem is that it is extremely smooth to begin
with. I would like to add some sub-micro features as a background so that I
am not generating a featureless gray photomicrograph. These features would
be essentially a topographic reference. I though of a mono layer of
particles dusted on the surface. I stated w/ common baking flour. The
particles were too large and not stuck to the surface so well (lots of edge
effect) so that one can't effectively have a good contrast of the spheres
and the background in the same micrograph. A very fine grid would also be
nice, but perhaps expensive. Smaller and flatter particles that were put in
solvent and allowed to dried might do the trick. Anybody have any
interesting suggestions or materials? I would be glad to share the results.
===========================================================
We faced a similar kind of question involving a Mylar film (some years ago),
which also was smooth and featureless at the SEM level and there was the
need to look for evidence of etching. In this case, the "etch" was exposure
to ozone. Our approach was the following:

a) Apply on one side of the film a special silicone resin; this was an
earlier version of the silicone used in our SPI Wet Replica Kit (see URL
http://www.2spi.com/catalog/spec_prep/wet_rep_kits.html )
The line between the resin covered side and the side without resin is quite
distinct. The resin thickness is about 0.5 to 1 mm.

b) Do your exposure of the film, but the silicone layer protects the one
side from exposure and it will not be harmed by the acid environment. Of
course, at some point it can ( I presume) be effected by acid so it has to
be a matter of time, temperature and pH.

c) After exposure, the silicone can be removed, and if indeed there is some
etching, if differences are seen one side of the interface vs. the other,
then you could draw some conclusions.

In those days, we found we needed ordinary Pt/C surface replication methods
(and TEM) to show the differences, one side to the other. But in the end
one is faced with having to prove the null hypothesis: If you see no effect
, does that mean no etching or does that mean one has not looked close
enough. Today, I would expect that AFM would have a better chance at seeing
differences that might not be seen with Pt/C surface replication.

Another approach, if we were to be doing this today, would be to try
applying the approach that has been used by Japanese researchers at Nagoya
University (if I remember correctly) who have been using thin osmium metal
layers, plasma coated, that are not more than ~1 nm thick, to replicate
macromolecules on flat surfaces. The method does work well for DNA for
example and might work well for a surface with a very subtle etch effect.
If you would like more information about this method, I could share
additional information with you about how this is done. I could also dig out
my notes on the people who are doing this work.

Disclaimer: SPI Supplies offers this type of silicone resin and also,
distributes the OPC-60 Osmium Plasma Coater made by Nippon Laser and
Electronics Labs, Nagoya, Japan.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Wed Jul 25 21:11:00 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 26 Jul 2001 14:01:54 GMT+1200
Subject: foreline traps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ie the anti-backstreaming trap between the mechanical pump and the
diff pump:-

is there a consensus out there on what's best?

Molecular seive, activated alumina pellets, or Cu gauze?

I've never been that happy about the first two because of mechanical
abrasion releasing abrasive powder into the rotary pump, particularly
from those Edwards holders that seem designed to mount directly on
the pump, where they get nicely and continuously vibrated.

And how do people regenerate each absorbent?

Heat in air to 600 deg C or so?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Jul 25 21:14:57 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 25 Jul 2001 19:10:09 -0700
Subject: Re: Inspection/assembly microscope recommendations?

Contents Retrieved from Microscopy Listserver Archives
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You might consider an Olympus SZ-40 system with a
Fostec fiber optic ACE ring light. There are several sources
for these 'scopes as used. The Fostec may be only available
as new (I have a near new light source I would sell...no ring
light bundle). I like the Fostec very much.

On the surplus market, Nikon stereo zooms come up quite
often. Maybe one of these would work for you. Try http://dovebid.com
for options.

gary g.

Disclaimer: No financial interest in any company referenced above.




At 02:31 PM 7/25/2001, you wrote:

} Our company needs to get a microscope for inspection and
} assembly/disassembly tasks. We would like it to have the following
} characteristics:
}
} 1) Stereo viewing
} 2) Wide field of view
} 3) Prefer zoom capabilities, but could take anything that would allow
} magnifications from about 4X to, say, 20X
} 4) Good working distance
}
} Would anyone care to make any recommendations or do you have any suggestions
} for us?
}
} Vendor input is welcome. Contact information follows my sig.
}
} Bruce Girrell
} Microline Technology Corp.
} 2397 Traversefield Dr.
} Traverse City, MI 49686
} http://www.microlinetc.com
}
} (231) 935-1585 (Voice)
} (231) 922-5099 (FAX)
} bigirrell-at-microlinetc.com



From daemon Thu Jul 26 07:18:49 2001



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Thu, 26 Jul 2001 07:05:50 -0500
Subject: Leitz Microhardness Tester.

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Anyone out there who can help me.
We want to 'refurbish' an old Leitz DURIMET Microhardness Tester for
routine applications by students. However, both objectives are badly
damaged and beyond repair. (No wonder, the unit is approximately 50
years old and had been used by numerous students in the past).
We would like to 'pick up' or purchase therefore the following types
of objectives (used or new):
Low Magnification 40x and High Magnification A 0.70 CHM 6.3 40x to
suit the DURIMET.
Thanks for any advise.

Hans Brinkies
Senior Lecturer
Swinburne University of Technology
School of Engineering and Science
Industrial Microscopy Laboratory
PO Box 218 - Hawthorn - Victoria - 3122 - Australia
Phone: +61 3 9214 8657 - FAX: +61 3 9214 8264


From daemon Thu Jul 26 10:11:14 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 26 Jul 2001 08:03:23 -0700 (PDT)
Subject: Re: labeling with antibodies--TEM

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Just my two cents concerning pre-embed labeling with gold damaging the
knife. We have done it for years and never noticed any damage.

Bob Underwood
Morphology Core
U of Washington

On Wed, 25 Jul 2001, Meghan Towner wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Good Afternoon!
} This is a follow up question involving tissue sections prepared for the TEM. My original question dealt with perfusion with physiological saline followed by cold 4% paraformaldhyde. I will be incubating the tissue (dorsal root ganglions) with an antibody. I have spoken to my advisor and Franklin Bailey at the UI EM center about preparing the tissue sections and eventual incubation with antibodies. I have received conflicting advice and am putting this out for more advice. Since I am going to use antibodies, does this change how I perfuse the animal? When incubating with the antibodies, should I do it pre- or postembedding? Immunogold labeling may be used and I have been told that preembedded labeling may damage the knife. I am open to other ways of labeling this tissue. Thank you.
}
} Meghan Towner
} Graduate Student
} Biological Sciences
} University of Idaho
}
}
}



From daemon Thu Jul 26 10:12:48 2001



From: ewens_bob-at-ti.com
Date: Thu, 26 Jul 2001 08:06:54 -0700
Subject: Unsubscribe

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Unsubscribe



From daemon Thu Jul 26 10:38:14 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 26 Jul 2001 08:31:03 -0700
Subject: Re: SEM of super smooth material

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Dear Smartech,
Just off the top of my head:
1. clay, ultra-soniced in alcohol and dropped on to dry.
2. the fine aluminum powder used in makeup, dusted on with a paintbrush or
dropped from alcohol as in 1.
3. small latex sheres used to calibrate TEMs. Already in suspension, just
drop on to dry.
I have used all three for various purposes. I have also used a very fine
grid (~1000 mesh) laid tight over the sample and then evaporated gold
through it. The resulting gold squares are quite visible in the SEM but
avoid the problems of edge effect.
At 02:50 PM 7/25/01 -0400, you wrote:

} I want so show that a polymer film has not been etched after exposure to an
} HCL based environment. The problem is that it is extremely smooth to begin
} with. I would like to add some sub-micro features as a background so that I
} am not generating a featureless gray photomicrograph. These features would
} be essentially a topographic reference. I though of a mono layer of
} particles dusted on the surface. I stated w/ common baking flour. The
} particles were too large and not stuck to the surface so well (lots of edge
} effect) so that one can't effectively have a good contrast of the spheres
} and the background in the same micrograph. A very fine grid would also be
} nice, but perhaps expensive. Smaller and flatter particles that were put in
} solvent and allowed to dried might do the trick. Anybody have any
} interesting suggestions or materials? I would be glad to share the results.
}
} SMARTech

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Jul 26 11:06:15 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 26 Jul 2001 12:06:16 -0400
Subject: RE: Foreline traps

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The various types of foreline traps are discussed in some detail on
pp. 146 - 149 of my book 'Vacuum Methods in Electron Microscopy"
(for a description see
http://www.2spi.com/catalog/abooks/book48.html/ and
http://www.pup.princeton.edu/titles/6484.html/)

At the time I was writing this book it appeared that perhaps the best
method of trapping oil vapors from the roughing pump was to use a
Micromaze trap produced by the Lesker company. These traps are
provided with a heater to be used for regenerating the trap, and it
appeared advantageous to run this heater continuously at about 75%
full power. Evidence available then indicated that when hot the
ceramic Micromaze material caused the oil molecules to break down
into small fragments that could pumped away. See above reference for
details.

Maybe some more efficient type of trap has been developed since, but
I have not heard of it.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Thu Jul 26 11:14:12 2001



From: Blanchette-Mackie, E. Joan Dr. (NIDDK) :      joanbm-at-bdg8.niddk.nih.gov
Date: Thu, 26 Jul 2001 11:58:52 -0400
Subject: TEST

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ljgkjfkjfkjfgk
.kjhk;jhkhkjh;kjh



From daemon Thu Jul 26 13:13:30 2001



From: jfb :      jfb-at-uidaho.edu
Date: Thu, 26 Jul 2001 11:06:31 -0700
Subject: Sucrose Gradient Centrifugation

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Does anyone remember the sucrose concentrations needed to form a
gradient for separating cells and cell fractions from media debris? We
once used this technique, but I have lost all of my references on the
procedure.
Thanks

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204



From daemon Thu Jul 26 14:11:29 2001



From: Mary Tyler :      mary-at-umit.maine.edu
Date: Thu, 26 Jul 2001 15:11:50 -0400
Subject: LM time-lapse video microscopy

Contents Retrieved from Microscopy Listserver Archives
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Dear Folks,
I'm in the process of setting up a system for doing time-lapse video
microscopy and would love to get information from others on what set-ups
work best for them. I'm interested in people's experience with time-lapse
VCRs and digital capture. I'm recording in color using a three-chip CCD
video camera. We have a Scion capture card on a Mac G4. I will be
recording over 24-hour periods.
In particular, has anyone been using NIH Image or Imagej to do time-lapse
in color?

I am new to this listserv, so my apologies if I'm raking over old
territory. I did do a search of the listserv archives and didn't find any
postings in this subject. Many thanks, Mary
===============================================
Mary_Tyler-at-umit.maine.edu or mtyler-at-maine.edu

Mary S. Tyler
Professor of Zoology
Dept. of Biological Sciences
University of Maine
Orono, ME 04469-5751
FAX: 207-581-2537



From daemon Thu Jul 26 15:04:03 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 26 Jul 2001 15:58:36 -0400
Subject: SEM of super smooth material

Contents Retrieved from Microscopy Listserver Archives
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You can smoke Mo in a number of ways. You can use wires or boats across the leads of an evaporator and do it in air, or you can take a wire and heat it with a Bunsen burner, or propane torch. The smoke should not be that hot, just don't keep your sample in the smoke or near the radiant heat for too long. The wire should continue to smoke a little just as you remove the heat. If you collect the smoke from Mg, you should be able to collect it far from the heat source as the smoke rises. I assumed in my original post that you would need high resolution imaging and small particles distributed with no carrier media to put the stuff on the surface. There was a response to my earlier post that said to scratch the surface, but that would produce features too gross.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
Sent: Wednesday, July 25, 2001 8:08 PM
To: Walck, Scott D.


I want so show that a polymer film has not been etched after exposure to an
HCL based environment. The problem is that it is extremely smooth to begin
with. I would like to add some sub-micro features as a background so that I
am not generating a featureless gray photomicrograph. These features would
be essentially a topographic reference. I though of a mono layer of
particles dusted on the surface. I stated w/ common baking flour. The
particles were too large and not stuck to the surface so well (lots of edge
effect) so that one can't effectively have a good contrast of the spheres
and the background in the same micrograph. A very fine grid would also be
nice, but perhaps expensive. Smaller and flatter particles that were put in
solvent and allowed to dried might do the trick. Anybody have any
interesting suggestions or materials? I would be glad to share the results.

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756


From daemon Thu Jul 26 15:38:56 2001



From: Elizabeth Dickey :      ecd10-at-psu.edu
Date: Thu, 26 Jul 2001 16:33:02 -0400
Subject: Postdoctoral Position

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POST-DOCTORAL POSITION
in
Electron Microscopy of Electroceramic Grain Boundaries

A postdoctoral position is available in the area of electroceramics
beginning September 1, 2001. The research project focuses on
understanding solute segregation at electroceramic grain boundaries,
in particular TiO2 and BaTiO3, and the ramifications for electrical
transport properties. Aspects of the project will be conducted under
the auspices of the Center for Dielectric Studies at the Pennsylvania
State University. Through a variety of electron imaging and
spectroscopy techniques we aim to quantify solute segregation and
grain boundary impedance as a function of doping level and
microstructure. For special boundaries we will obtain atomic
structure and site-specific segregation information that will be used
as the basis for theoretical calculations. The ideal candidates for
this position will have experience in HREM, STEM, EDS and/or EELS and
have an understanding of grain boundary phenomena in electroceramics.
The salary will be commensurate with qualifications and experience.

Please forward questions or send applications to Professor Elizabeth Dickey:

Department of Materials Science and Engineering
The Pennsylvania State University
195 Materials Research Institute Building
University Park, PA 16802-7003

tel: (814) 865-9067
FAX: (814) 863-8561
email: ecd10-at-psu.edu
--
Elizabeth Dickey
Department of Materials Science and Engineering
Pennsylvania State University
195 Materials Research Institute Building
University Park, PA 16802-7003

tel: (814)865-9067
fax: (814)863-8561
email: ecd10-at-psu.edu


From daemon Thu Jul 26 17:07:59 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 26 Jul 2001 14:59:58 -0700
Subject: LM: DAPI excitation filter damage?

Contents Retrieved from Microscopy Listserver Archives
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Hi:

A colleague has asked if I could help explain why the excitation filter in
his DAPI filter set on a Zeiss Axiophot deteriorates after a few months of
use.

Excitation filters in other sets last longer and don't show this problem.
The symptoms are uneven illumination that shows up in the image and an
obvious 'burned ' look to the filter.

His questions are like: Is this normal? If not, what is wrong? How can I
avoid this problem?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Jul 26 17:58:39 2001



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Fri, 27 Jul 2001 18:57:17 -0400
Subject: Re: foreline traps

Contents Retrieved from Microscopy Listserver Archives
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I have a couple Micromaze bakeable foreline traps that Lesker sells. They
were on a TEM that I used to own. I almost forgot that I had these. I kept
them with the intention of trying to sell them someday. These two were
modified for safety reasons and so nothing around them would get damaged by
the heat from these. These traps when turned on get pretty hot if I remember
right. For these reasons longer stainless pipes were welded on and a cage
was built around each one. I would recommend something like this if you use
these traps.

Not only are the maze traps better, I think, but they are easier to use. Use
to simply plug them into an AC outlet overnight once a week to bake them
out. Kept the vacuum system running while bakeing.

Joel McClintock
EM Specialist
U of Kentucky

At 02:01 PM 7/26/01 GMT+1200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jul 26 18:20:12 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 26 Jul 2001 16:14:59 -0700
Subject: Re: Sucrose Gradient Centrifugation

Contents Retrieved from Microscopy Listserver Archives
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It's better to use PERCOLL solution. It's isoosmotic and you could
separate cells from microsomal fraction or viruses etc. You may also
separate different cell types by this technique. PERCOLL was manufactured
by Pharmacia, which is a part of Amersham now, I believe. Good luck. Sergey

At 11:06 AM 7/26/01 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Jul 26 20:01:16 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Thu, 26 Jul 2001 18:54:21 -0600 (MDT)
Subject: Re: Sucrose Gradient Centrifugation

Contents Retrieved from Microscopy Listserver Archives
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I have a question about using Percoll for TEM - someone told me just last
week that you shouldn't use Percoll if you'll be doing thin sections,
since it is a silica (can damage knives) - has anyone used it and either
seen or not seen gunk in their sections? Scratches &/or damaged a knife?
Sea creatures or other beach denizens? (Sorry, I'm tired and couldn't
resist)

I'm not planning to use it any time soon, but hearing about it again makes
me curious about using it to prep for EM. I've seen nasty sucrose crystals
a few times with samples from sucrose gradients, but they are mostly an
annoyance.

Thanks!

Tamara


On Thu, 26 Jul 2001, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} It's better to use PERCOLL solution. It's isoosmotic and you could
} separate cells from microsomal fraction or viruses etc. You may also
} separate different cell types by this technique. PERCOLL was manufactured
} by Pharmacia, which is a part of Amersham now, I believe. Good luck. Sergey
}
} At 11:06 AM 7/26/01 -0700, you wrote:

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Fri Jul 27 03:36:07 2001



From: Steve Chapman :      PROTRAIN-at-compuserve.com
Date: Fri, 27 Jul 2001 04:28:12 -0400
Subject: SEM of super smooth material

Contents Retrieved from Microscopy Listserver Archives
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Hi

I agree with the ideas of placing simple structures on the surface of the
specimen, Mn smoke or polystyrene latex. However general tips for
featureless specimens are -

1. Keep the kV down ( {5kV) - to restrict the probe to a true surface
investigation

2. Use high tilt (} 45deg) as the small amount of backscatter present
will serve to emphasize topography.

Happy scanning

Steve Chapman
Senior Consultant Protrain
For professional training in electron microscopy world wide
www.emcourses.com


From daemon Fri Jul 27 05:29:17 2001



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Fri, 27 Jul 2001 12:29:03 +0200
Subject: Re: Sucrose Gradient Centrifugation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For what its worth:

Some time ago we tried to section some epoxy-embedded,
Percoll-separated cell fractions. This resulted in very evident damage
to glass knife cutting edges - to such an extent that we decided not
to jeopardize a diamond knife.

Separating the fractions on a sucrose gradient resulted in blocks that
sectioned without any problems.


Jan Coetzee

Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


Tamara Howard wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have a question about using Percoll for TEM - someone told me just last
} week that you shouldn't use Percoll if you'll be doing thin sections,
} since it is a silica (can damage knives) - has anyone used it and either
} seen or not seen gunk in their sections? Scratches &/or damaged a knife?
} Sea creatures or other beach denizens? (Sorry, I'm tired and couldn't
} resist)
}
} I'm not planning to use it any time soon, but hearing about it again makes
} me curious about using it to prep for EM. I've seen nasty sucrose crystals
} a few times with samples from sucrose gradients, but they are mostly an
} annoyance.
}
} Thanks!
}
} Tamara
}
} On Thu, 26 Jul 2001, Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It's better to use PERCOLL solution. It's isoosmotic and you could
} } separate cells from microsomal fraction or viruses etc. You may also
} } separate different cell types by this technique. PERCOLL was manufactured
} } by Pharmacia, which is a part of Amersham now, I believe. Good luck. Sergey
} }
} } At 11:06 AM 7/26/01 -0700, you wrote:
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|


From daemon Fri Jul 27 07:08:07 2001



From: Alun :      alun-at-deepsouth.co.nz
Date: Fri, 27 Jul 2001 07:02:58 -0500
Subject: Re:SEM minerals

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Has anyone had any experience with SEM photographing minerals,
or had a problem in obtaining a vacuum in the case of clay type minerals.

Bernard.





From daemon Fri Jul 27 09:27:22 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Fri, 27 Jul 2001 10:19:18 -0400
Subject: Professor position available in Materials

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School of Mechanical, Materials, Manufacturing Engineering & Management
Advanced Materials Research Group
University of Nottingham, UK

Professor of Materials

Applications are invited for the above post from candidates with an
outstanding record of research to reinforce and add to existing research
strengths and providing a third professorial post in the Advanced Materials
Group. Candidates should have expertise in one of the following areas:
electron microscopy applied to the processing of advanced materials;
surface engineering of materials; near-net-shape processing of materials or
biomaterials

The person appointed will be expected to contribute to the teaching of
courses within the School, which was graded 4A in the Research Assessment
Exercise and gained excellent scores for its teaching quality.

Salary will be within the professional range, minimum £41,089 per annum.

Informal enquiries may be addressed to Professor D G McCartney or Professor
A B Seddon, tel: 0115 951 3740, Email: Angela.Seddon-at-Nottingham.ac.uk
Further details of the School are available on the WWW at:
http://www.nottingham.ac.uk/school4m/.

Further details and application forms are available on the WWW
at: http://www.nottingham.ac.uk/personnel/ or from the Personnel
Office, Highfield House, The University of Nottingham, University Park,
Nottingham, NG7 2RD. Tel: 0115 951 3266. Fax: 0115 951 5215. Email:
June.Pedlar-at-Nottingham.ac.uk. Please quote ref. MCM/165. Closing date: 15
September 2001.

…………………………………………………………………………………………………………

To be published as follows:

Guardian - Tuesday 24 July
THES - Friday 27 July
Jobs.ac.uk - w/c Monday 23 July




From daemon Fri Jul 27 10:02:24 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 7/27/01 12:29 PM
Subject: Re: Sucrose Gradient Centrifugation

Contents Retrieved from Microscopy Listserver Archives
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Back in the early to mid 80's, I used Ficoll-Hypaque to layer out
different white cells from peripheral blood. Never had a problem with
sugar crystals. Only damage to the diamond knife was (cringe) user
error.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


For what its worth:

Some time ago we tried to section some epoxy-embedded,
Percoll-separated cell fractions. This resulted in very evident damage
to glass knife cutting edges - to such an extent that we decided not
to jeopardize a diamond knife.

Separating the fractions on a sucrose gradient resulted in blocks that
sectioned without any problems.


Jan Coetzee

Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


Tamara Howard wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have a question about using Percoll for TEM - someone told me just last
} week that you shouldn't use Percoll if you'll be doing thin sections,
} since it is a silica (can damage knives) - has anyone used it and either
} seen or not seen gunk in their sections? Scratches &/or damaged a knife?
} Sea creatures or other beach denizens? (Sorry, I'm tired and couldn't
} resist)
}
} I'm not planning to use it any time soon, but hearing about it again makes
} me curious about using it to prep for EM. I've seen nasty sucrose crystals
} a few times with samples from sucrose gradients, but they are mostly an
} annoyance.
}
} Thanks!
}
} Tamara
}
} On Thu, 26 Jul 2001, Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It's better to use PERCOLL solution. It's isoosmotic and you could
} } separate cells from microsomal fraction or viruses etc. You may also
} } separate different cell types by this technique. PERCOLL was manufactured
} } by Pharmacia, which is a part of Amersham now, I believe. Good luck. Serge
y
} }
} } At 11:06 AM 7/26/01 -0700, you wrote:
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|





From daemon Fri Jul 27 11:40:34 2001



From: Lifeng Dong :      lifengd-at-pdx.edu
Date: Fri, 27 Jul 2001 09:33:16 -0700 (PDT)
Subject: Femail roommate wanted: Microscopy & Microanalysis 2001

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To: Microscopy-at-sparc5.microscopy.com


Roommate wanted:

I have made a reservation at Hotel Queen Mary in Long Beach, California for the

Microscopy and Microanalysis 2001 Conference from August 5 – 9. It turns out
the room has two double beds. If any female colleagues (one female) who want to

share the room with me, please contact me by e-mail (jiaoj-at-pdx.edu). The room
is reserved for 4 nights ($115/night for two persons) from August 5 – August 9.


Dr. Jun Jiao
Assistant Professor
Physics Department
Portland State University
Portland, Oregon, U.S.A.




From daemon Fri Jul 27 11:44:27 2001



From: Alan J. Kruger :      kruger-at-email.marc.usda.gov
Date: Fri, 27 Jul 2001 10:41:51 -0500
Subject: Re: Sucrose Gradient Centrifugation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara,

I have used Percoll for leydig cell seperations and TEM with no knife damage.
After the isolation procedure I have used a series of buffer washes before
dehydration and embedding procedures.

Al Kruger
Reproduction Research Unit
USDA-ARS-MARC
Clay Center, NE 68901

Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have a question about using Percoll for TEM - someone told me just last
} week that you shouldn't use Percoll if you'll be doing thin sections,
} since it is a silica (can damage knives) - has anyone used it and either
} seen or not seen gunk in their sections? Scratches &/or damaged a knife?
} Sea creatures or other beach denizens? (Sorry, I'm tired and couldn't
} resist)
}
} I'm not planning to use it any time soon, but hearing about it again makes
} me curious about using it to prep for EM. I've seen nasty sucrose crystals
} a few times with samples from sucrose gradients, but they are mostly an
} annoyance.
}
} Thanks!
}
} Tamara
}
} On Thu, 26 Jul 2001, Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It's better to use PERCOLL solution. It's isoosmotic and you could
} } separate cells from microsomal fraction or viruses etc. You may also
} } separate different cell types by this technique. PERCOLL was manufactured
} } by Pharmacia, which is a part of Amersham now, I believe. Good luck. Sergey
} }
} } At 11:06 AM 7/26/01 -0700, you wrote:
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|



From daemon Fri Jul 27 12:10:49 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 27 Jul 2001 13:04:38 -0400
Subject: RE: LM time-lapse video microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mary,
Looking for something else, I found the following. Seems relevant.

http://hamon.swmed.edu/~jwaddle/software/jimage4d/overviewdoc.html

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
Schedule: http://bio.wcupa.edu/cgi-bin/webreserve.acgi
Please call before visiting.


} ----------
} From: Mary Tyler
} Sent: Thursday, July 26, 2001 3:11 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM time-lapse video microscopy
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Folks,
} I'm in the process of setting up a system for doing time-lapse video
} microscopy and would love to get information from others on what set-ups
} work best for them. I'm interested in people's experience with time-lapse
} VCRs and digital capture. I'm recording in color using a three-chip CCD
} video camera. We have a Scion capture card on a Mac G4. I will be
} recording over 24-hour periods.
} In particular, has anyone been using NIH Image or Imagej to do time-lapse
} in color?
}
} I am new to this listserv, so my apologies if I'm raking over old
} territory. I did do a search of the listserv archives and didn't find any
} postings in this subject. Many thanks, Mary
} ===============================================
} Mary_Tyler-at-umit.maine.edu or mtyler-at-maine.edu
}
} Mary S. Tyler
} Professor of Zoology
} Dept. of Biological Sciences
} University of Maine
} Orono, ME 04469-5751
} FAX: 207-581-2537
}
}
}


From daemon Fri Jul 27 13:25:14 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 27 Jul 2001 14:17:05 -0400
Subject: RE: DAPI excitation filter damage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the old days, filters could be 'burned' out by the high voltage Hg lamp
if there wasn't a proper buffer. Is the bulb correct for the illuminator?
Sometimes these days, a neutral density filter is placed between the
illuminator and the filter set.

Check with Zeiss - they will answer.

URL:
http://www.zeiss.de/us/micro/home.nsf/allBySubject/Launch+-+Zeiss-engl+Notes
Template

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
Schedule: http://bio.wcupa.edu/cgi-bin/webreserve.acgi
Please call before visiting.


} ----------
} From: jmkrupp-at-cats.ucsc.edu
} Sent: Thursday, July 26, 2001 5:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM: DAPI excitation filter damage?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} A colleague has asked if I could help explain why the excitation filter in
} his DAPI filter set on a Zeiss Axiophot deteriorates after a few months
} of
} use.
}
} Excitation filters in other sets last longer and don't show this problem.
} The symptoms are uneven illumination that shows up in the image and an
} obvious 'burned ' look to the filter.
}
} His questions are like: Is this normal? If not, what is wrong? How can I
} avoid this problem?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}


From daemon Fri Jul 27 13:36:31 2001



From: Eric :      biology-at-ucla.edu
Date: Fri, 27 Jul 2001 11:28:21 -0700
Subject: Sloutins for UA schmutz on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the Microscopy List,


First I would like to thank everyone for their help and suggestions =
with my problem of "dirty grids" or as I call it (technical term) ka ka =
on the sections.

I know it has been a while since I asked this question, but I have not =
been able to go through and try out all the suggestions sent to me .


The solvent I am using is a mixture of 50% Methanol and 50% ddH20
Staining was done on dental wax for 10 minutes.

For lead staining it was done for 5 minutes with sodium hydroxide =
pellets in a covered petri dish.

Everything was filtered through .45 micron filters.


Solutions I was given for my staining artifact were:

1. Change filter size to .20 microns.
2. Use parafilm instead of dental wax for staining.
3. Shorten my staining time from 10 minutes to 5 minutes.
4. Change suppliers of UA stain
5. Try a new batch of stain.


Solutions I tried.

1. Used parafilm instead of Dental wax
2. used .20 micron filters
3. mixed up a new batch of stain.

By changing all these criteria I fixed the staining problem that
occurred a few weeks ago.

Now I get about 90-95% clean grids. Sometimes there is a little
schmutz around, but not like occurred before.

Thanks to everyone for their suggestions

Eric A. Rosen
UCLA Medical Center
Electron Microscopy Lab
Dept. Pathology and Lab Medicine
Los Angeles, CA 90095
310-825-5737





From daemon Fri Jul 27 13:56:40 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 27 Jul 2001 14:54:36 -0400
Subject: RE: SEM minerals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bernard,

I haven't had trouble with clays but certainly with gypsum (CaSO4 2H2O). I
imagine a very hydrous clay would cause similar problems achieving vacuum. You
could dehydrate the sample in a (vacuum) oven, but then of course you're
looking at a different clay from the one you started with. Some variety of
Environmental or Cryo-SEM might be required, depending on your needs.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Friday, July 27, 2001 8:03 AM, Alun [SMTP:alun-at-deepsouth.co.nz] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone had any experience with SEM photographing minerals,
} or had a problem in obtaining a vacuum in the case of clay type minerals.
}
} Bernard.
}
}



From daemon Sat Jul 28 03:05:14 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 28 Jul 2001 17:57:50 +1000
Subject: RE: Solutions for UA schmutz on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't think that filtering achieves much since that will only eliminate the
boulders; 0.5um rocks are 5mm at only 10,000x mag. Use 2x distilled water.

UA in methanol goes off much faster than UA in water and that may be your major
problem. Made up in water I trust UA for one month, when refrigerated and
mostly kept in the dark. Made up in methanol, I would trust that solution only
for a day or two.

The second problem is over-staining. Ten or even twenty minutes for aqueous UA
is normal, but methanolic UA may be over-staining after 2 minutes or so.

I like your technical term "schmutz" - for the uninitiated, schmutzig is German
for "dirty" and schmutz means dirt or just muck. I vote that "schmutz" should
be official jargon in microscopy.
Another little hint: We all have noticed that a grid when applied to a drop of
staining solution floats to the top. So does all the schmutz. It makes sense to
apply a grid to the side of a drop, that way any top floating schmutz is not
picked up but pushed away. With aqueous UA and lead stain it makes sense to
only stain one side of the section. Methanolic stains may not support the grid
and the grid must be placed into the drop. This increased the chance of
contamination and also causes more intense staining. More intense staining may
sound desirable, but there are some negatives. This contributes to
over-staining, also, some stains only penetrate part of a section and so give
the effect of a thinner section and sharper image.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, July 28, 2001 4:28 AM, Eric [SMTP:biology-at-ucla.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To the Microscopy List,
}
}
} First I would like to thank everyone for their help and suggestions =
} with my problem of "dirty grids" or as I call it (technical term) ka ka =
} on the sections.
}
} I know it has been a while since I asked this question, but I have not =
} been able to go through and try out all the suggestions sent to me .
}
}
} The solvent I am using is a mixture of 50% Methanol and 50% ddH20
} Staining was done on dental wax for 10 minutes.
}
} For lead staining it was done for 5 minutes with sodium hydroxide =
} pellets in a covered petri dish.
}
} Everything was filtered through .45 micron filters.
}
}
} Solutions I was given for my staining artifact were:
}
} 1. Change filter size to .20 microns.
} 2. Use parafilm instead of dental wax for staining.
} 3. Shorten my staining time from 10 minutes to 5 minutes.
} 4. Change suppliers of UA stain
} 5. Try a new batch of stain.
}
}
} Solutions I tried.
}
} 1. Used parafilm instead of Dental wax
} 2. used .20 micron filters
} 3. mixed up a new batch of stain.
}
} By changing all these criteria I fixed the staining problem that
} occurred a few weeks ago.
}
} Now I get about 90-95% clean grids. Sometimes there is a little
} schmutz around, but not like occurred before.
}
} Thanks to everyone for their suggestions
}
} Eric A. Rosen
} UCLA Medical Center
} Electron Microscopy Lab
} Dept. Pathology and Lab Medicine
} Los Angeles, CA 90095
} 310-825-5737
}
}



From daemon Sat Jul 28 04:26:47 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 28 Jul 2001 19:21:41 +1000
Subject: computer virus alert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know that this server and most universities and other large institutions are
virus protected, but many readers will be interested to learn that my usual
tally of (eliminated) virus messages is about 2 per week. During the last ten
days this has blown out 26.
My server scans for and eliminates the virus part of a message and we have our
own anti virus system.
Just now, a lot of people out there are badly affected by the
TROJ_SIRCAM.A virus and its variants. All of these message come in with
different headers and all these files terminate with these extensions: exe,
com, pif, bat, 1nk, scr.
I received a couple of virus messages from SE Asian Universities.

Incidentally: That amazing Nigerian scam was supposedly squashed. Hmmm,
I used to get one or two messages weekly, now I get ten or more from them and
some clones. Trouble is that emails are too cheap to send and since no
legislator is tough on spaming, we all must waste our time (and some fools part
with their money).
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com



From daemon Mon Jul 30 02:40:22 2001



From: Mark G BLACKFORD :      mgb-at-ansto.gov.au
Date: Mon, 30 Jul 2001 17:19:26 +1000
Subject: 2010F emitter question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

I was wondering if anyone could offer some advice regarding my 2010F gun.

I periodically measure beam current using the small viewing screen after
aligning the microscope in a reproducible configuration (A1 and A2 set at
specific values, 50micron condenser aperture, CBD mode 1nm alpha 9, 1MX
magnification with beam focused using Brightness knob). Over the last
several months I have observed that the size of the spot is approximately 4
times larger than when the microscope was commissioned in early 1999. The
FWHM of the probe was measured yesterday to be 2.2nm. The emission timer
reads 19,200 hrs.

Obviously our analytical capabilities are compromised by the gun's current
condition but we can still perform the majority of our work.

Is the probe size likely to continue to get larger?

Is our emitter about to expire completely?

What are the chances of the gun lasting another 6 to 12 months without the
probe deteriorating dramatically?

Thanks for any advice you can offer, cheers


Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.


From daemon Mon Jul 30 08:05:11 2001



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Mon, 30 Jul 2001 08:56:43 -0400
Subject: wanted - JEOL 100CX parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings;

We are seeking to buy an operational 100CX objective lens power supply.
Please contact me off line at the address below if you have one for sale.

Thanks in advance.

Owen

Owen P. Mills
Electron Optics Facility Engineer
Michigan Technological University
Department of Materials Science and Engineering
Rm 512 M&M Building
Houghton, Mi 49931
Ph: 906-487-2002
FAX: 906-487-2934
Email: opmills-at-mtu.edu
URL: http://www.mm.mtu.edu/~opmills/index.html



From daemon Mon Jul 30 08:14:32 2001



From: Martin Roe :      Martin.Roe-at-nottingham.ac.uk
Date: Mon, 30 Jul 2001 14:08:10 +0100
Subject: 16 bit ACDSEE for 3.11 os

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello fellow microscopists,
has anyone out there know how I can get the free trial version ACDSee 16 bit installation software suitable for Win 3.11 operating system? I tried getting it from their web site but it appears they have discontinued it -only programs suitable for Win 95 and higher.I know that until recently this free down loadable version was available on their website. I have tried contacting their helpline, but so far to no avail.
I'd be very grateful to anyone who could contact me off-line with information where I can get it. Or perhaps does anyone know of something very similar -allowing very quick viewing of images in file manager of 3.11?
Regards
Martin Roe





From daemon Mon Jul 30 08:19:13 2001



From: Martin Roe :      Martin.Roe-at-nottingham.ac.uk
Date: Mon, 30 Jul 2001 14:13:02 +0100
Subject: SEM: ACDsee for 3.11 os (16 bit)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello fellow microscopists,
has anyone out there know how I can get the free trial version ACDSee 16 bit installation software suitable for Win 3.11 operating system? I tried getting it from their web site but it appears they have discontinued it -only programs suitable for Win 95 and higher.I know that until recently this free down loadable version was available on their website. I have tried contacting their helpline, but so far to no avail.
I'd be very grateful to anyone who could contact me off-line with information where I can get it. Or perhaps does anyone know of something very similar -allowing very quick viewing of images in file manager of 3.11?
Regards
Martin Roe



From daemon Mon Jul 30 09:10:50 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 30 Jul 2001 15:10:34 +0100 (GMT Daylight Time)
Subject: Re: 2010F emitter question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mark,

I read your message just after carrying out my monthly
emitter checks on our 3000F at 20,386 hours. We also hope
to predict the degradation in emitter performance and plan
a service replacement for a few minutes before the tip
fails:-) Well the're not cheap are they?

I have a slightly different set up; I adjust A1 to get
200uA emission and record similar data to you. I also
record the beam current on a picoammeter. I don't measure
the spot size accurately but I note the magnification
required to fill the centre spot on the small screen. It's
a subjective test but the result has been either 1, 1.2 or
1.5M times at every test and the spot does not show any
trend towards getting larger.

What I have noticed is that the spot size is very dependent
on the objective lens setting (now standardised) and beam
tilt (rotation centre). If there is any objective
astigmatism then it is 'corrected' by the condenser
stigmators giving a larger spot.

If you do measure the current on the small screen you may
find that it alters with both probe size and position on
the screen (different absorption at different positions).

Make sure you are at DV=0 (or -14 in our case to allow for
the GIF voltage offset) and that the column is always fully
aligned in the mode you use (in our case CBD, alpha 9, spot
0.4nm and smallest C aperture).

I know that Sheffield University were monitoring their
2010F emitter but I don't think that they got any warning
of a tip failure before their's was replaced.

Maybe others have better information for you.

Regards,
Ron

On Mon, 30 Jul 2001 17:19:26 +1000 Mark G BLACKFORD
{mgb-at-ansto.gov.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
}
} I was wondering if anyone could offer some advice regarding my 2010F gun.
}
} I periodically measure beam current using the small viewing screen after
} aligning the microscope in a reproducible configuration (A1 and A2 set at
} specific values, 50micron condenser aperture, CBD mode 1nm alpha 9, 1MX
} magnification with beam focused using Brightness knob). Over the last
} several months I have observed that the size of the spot is approximately 4
} times larger than when the microscope was commissioned in early 1999. The
} FWHM of the probe was measured yesterday to be 2.2nm. The emission timer
} reads 19,200 hrs.
}
} Obviously our analytical capabilities are compromised by the gun's current
} condition but we can still perform the majority of our work.
}
} Is the probe size likely to continue to get larger?
}
} Is our emitter about to expire completely?
}
} What are the chances of the gun lasting another 6 to 12 months without the
} probe deteriorating dramatically?
}
} Thanks for any advice you can offer, cheers
}
}
} Mark Blackford
} Materials Division, ANSTO
} PMB 1,
} Menai, N.S.W., 2234
} Australia
}
} Phone 61 2 9717 3027
} Fax 61 2 9543 7179
}
} Disclaimer:
} The views expressed in this E-mail message do not necessarily represent the
} official views of ANSTO from which this message was conveyed.
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk





From daemon Mon Jul 30 10:22:43 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Mon, 30 Jul 2001 10:17:02 -0500 (CDT)
Subject: CCD for 35mm port of PEELS compatible

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering options for a CCD on a 35mm port or in
the PEELS compatible location of a Gatan PEELS. Any input,
including from vendors, would be useful. This would be
for HREM applications.

-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html




From daemon Mon Jul 30 11:19:00 2001



From: Svetla Stoilova-McPhie :      svetla-at-burnham.org
Date: Mon, 30 Jul 2001 09:13:07 -0700
Subject: Re: Solutions for UA schmutz on grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are you aware that in some german dialects "Schmutz" does means other
things that dirt?
And accidentally some people have family names as Schmutz and even worst
they happen to be quite good microscopists using this server as well.
May be some latin name or older "dead" language name would help? Or why
not leva is as dirt?
Just a suggestion.
svetla
--
Svetla Stoilova-McPhie, PhD
The Burnham Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037
tel: 858 646 3100 ext.3484, fax: 858 646 3196


From daemon Mon Jul 30 11:25:57 2001



From: BuiLTAngEL-at-aol.com
Date: Mon, 30 Jul 2001 12:20:29 EDT
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ubsubcribe


From daemon Mon Jul 30 13:32:40 2001



From: Don Gantz :      Gantz-at-med-biophd.bu.edu
Date: Mon, 30 Jul 2001 14:24:39 -0400
Subject: Preparation of Araldite sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
All of my previous experience in preparing thick and thin sections for
LM and TEM, respectively, is with epon. Should I expect similar behavior
with araldite when sectioning with a diamond knife and staining with
toluidine blue or aqueous UA and Reynolds lead citrate? I would appreciate
any tips or references. Thanks.

Donald Gantz
Dept. Physiology & Biophysics
Boston University School of Medicine
Boston, Massachusetts 02118
Email: Gantz-at-Biophysics.bumc.bu.edu
Phone: 617-638-4017




From daemon Mon Jul 30 13:51:52 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 30 Jul 2001 13:46:52 -0500
Subject: MSA Archivist Position: Last Call

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy Society of America is seeking an Archivist to manage
the historical documents of our Society. This position is appointed
by MSA Council for a period of three years.

We are looking for someone who is experienced in the storage and
cataloging of (primarily) paper documents. Although this is a
voluntary position (non-paying), the Society would provide funding
for expendibles and day-to-day operations (and possibly a part-time,
student-worker assistant).

If interested (or if you know of a qualified person), please contact
me at the M&M 2001 meeting in Long Beach next week or at the
following address:


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Mon Jul 30 14:47:09 2001



From: SEM Machine :      SEM-at-ACATC.AME.Arizona.edu
Date: Mon, 30 Jul 2001 12:41:57 -0700
Subject: Replacement Vacuum Pumps - Need Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Hitachi S-650 with two 160VP pumps that need to be replaced. I
have called Hitachi about information on the pumps, but they are having some
difficulty in locating any specs on them. Searching the Internet did not
give any information either.

The pumps plug into what appears to be a standard 3-prong socket and the
pumps have information on the housing for 110 V, so I'm thinking that the
power requirements are fairly standard. What I don't know is what flow
rate, et cetera, to get for the replacement pumps.

Does anyone know where I could locate the pump specs or could you recommend
some pumps that would work? And, in case you haven't figured it out
already, I'm not an expert on vacuum systems, so I may be going about this
the wrong way by looking at specs that really don't matter. If someone
could let me know what parameters are the important things to look for, I'd
be grateful.

Thanks,

-Norman Kay
AME Dept.
The Univ. of Arizona







From daemon Mon Jul 30 15:39:08 2001



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Mon, 30 Jul 2001 15:33:01 -0500
Subject: TEM_Materials: Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Research Associate in Quantitative Electron Microscopy of
Nano-Structures

Applications are invited for a postdoctoral research associate position at
the Dept. of Materials Science and Engineering, University of
Illinois-Urbana/Champaign. The position involves developing electron
diffraction and spectroscopy techniques for quantitative characterization of
the electronic and structural properties of nano-structures, using the state
of art electron microscopy facilities (JEOL 2010F-GIF and VG HB501-STEM) at
the Center for Microanalysis of Materials. The successful candidate should
have strong experience in advanced materials electron microscopy and
diffraction techniques. Experience with STEM, energy-filtered imaging and
diffraction, and electron energy-loss spectroscopy is desired, but not
required. Preference will be given to candidates with strong experimental
aptitude. A PhD. in Physics, Materials Science or related field is required.
The position is funded by DOE and is initially for one year with possibility
for renewal up to 3.5 years. Applicants should send a curriculum vitae,
including a list of publications and research experience, and the names of
three references to:
Prof. J.M. Zuo,
Dept. of Materials Science and Engineering,
University of Illinois at Urbana and Champaign,
1304 W Green Street, Urbana, IL 61801;
217-333-2736 (fax); (217) 244-6504 (phone);
jianzuo-at-uiuc.edu (email).

Application materials should be received before Oct. 1, 2001 for full
consideration.






From daemon Mon Jul 30 16:12:27 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Mon, 30 Jul 2001 14:05:27 -0700
Subject: Re: LM time-lapse video microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mary,
As you've been told, NIH Image will do this, saving the timelapse images as a
stack held in RAM. However, Object Image, the extended version of NIH Image
(avalilable from simon.bio.uva.nl), will do it with a means to create a
similar control window to add integration or averaging, and to save the image
series to hard drive. I will send you the macro for Object Image directly.

Regards,
Glen

Mary Tyler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Folks,
} I'm in the process of setting up a system for doing time-lapse video
} microscopy and would love to get information from others on what set-ups
} work best for them. I'm interested in people's experience with time-lapse
} VCRs and digital capture. I'm recording in color using a three-chip CCD
} video camera. We have a Scion capture card on a Mac G4. I will be
} recording over 24-hour periods.
} In particular, has anyone been using NIH Image or Imagej to do time-lapse
} in color?
}
} I am new to this listserv, so my apologies if I'm raking over old
} territory. I did do a search of the listserv archives and didn't find any
} postings in this subject. Many thanks, Mary
} ===============================================
} Mary_Tyler-at-umit.maine.edu or mtyler-at-maine.edu
}
} Mary S. Tyler
} Professor of Zoology
} Dept. of Biological Sciences
} University of Maine
} Orono, ME 04469-5751
} FAX: 207-581-2537



From daemon Mon Jul 30 19:25:53 2001



From: Ann P. Ketter :      kettera-at-bcc.orst.edu
Date: Mon, 30 Jul 2001 17:17:42 -0700
Subject: LM- what does evan's blue stain?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am working with apoptoic cells in plant tissues. I am using Evan's
Blue as a test of viability and of apoptosis. Can anyone tell me what
Evan's blue (EB) stains in the cell? I know that it can not enter a
cell with an intact membrane but when it enters a damaged or dead cell
what does the EB actually bind to?
Ann



From daemon Mon Jul 30 19:45:39 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 30 Jul 2001 17:40:50 -0700
Subject: Re: Replacement Vacuum Pumps - Need Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Duniway Stock Room.

http://www.duniway.com

They are pretty good in locating info and fixing all
sorts of items. They do mech, ion and diffusion pump
rebuilds. Very good service and high quality.

gary g.


At 12:41 PM 7/30/2001, you wrote:

} We have a Hitachi S-650 with two 160VP pumps that need to be replaced. I
} have called Hitachi about information on the pumps, but they are having some
} difficulty in locating any specs on them. Searching the Internet did not
} give any information either.
}
} The pumps plug into what appears to be a standard 3-prong socket and the
} pumps have information on the housing for 110 V, so I'm thinking that the
} power requirements are fairly standard. What I don't know is what flow
} rate, et cetera, to get for the replacement pumps.
}
} Does anyone know where I could locate the pump specs or could you recommend
} some pumps that would work? And, in case you haven't figured it out
} already, I'm not an expert on vacuum systems, so I may be going about this
} the wrong way by looking at specs that really don't matter. If someone
} could let me know what parameters are the important things to look for, I'd
} be grateful.
}
} Thanks,
}
} -Norman Kay
} AME Dept.
} The Univ. of Arizona



From daemon Mon Jul 30 20:45:07 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 30 Jul 2001 15:37:56 -1000 (HST)
Subject: M&M 2001 Exhibitor Demonstrations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy and Microanalysis 2001 in Long Beach-

Once again the MSA Education Committee is organizing Exhibitor
Demonstrations on Tuesday, August 7 from 5:00 to 6:00pm in the Exhibit
Hall. These mini-seminars or tutorial demonstrations are held in the
booths of the participating companies after the Hall is closed to
non-participants at 5:00 pm.

Signup sheets with titles and descriptions are at the MSA Education table
in the MSA Mega Booth. When you sign up you will be issued a ticket, which
you will need to renter the Hall after it is closed. You need to sign
up no later than noon on Tuesday. The number of attendees is limited,
so visit the MSA Education table soon, since the demonstrations get filled
up quickly!

Here's a list of participating Exhibitors and titles:

Asylum Research
Force Measurements 101 - Featuring the New, Low Noise BioLever

Carl Zeiss, Inc., Microscopy & Imaging Systems
Zeiss LSM 5 PASCAL Personal Confocal Microscope Features and
Capabilities.

Digital Instruments/Veeco Metrology Group
Recent Advances in Throughput, Resolution, Sensitivity, and
Functionality with Atomic Force/Scanning Probe Microscopy

Evex Analytical
Video SEM

FEI Company
High Resolution STEM Imaging on a TEM

Gatan, Inc.
Fast Mapping with Hi-Speed EELS Spectrum Imaging

Hamamatsu Photonic Systems
Hamamatsu Introduces IEEE1394

Hitachi Scientific Instruments
Hitachi's NEW WxB Filter System on the S-4700 FESEM

IATIA Group
Optical Phase Microscopy with QPm: Quantitative Imaging and
Conventional Visualization Analogs

IXRF Systems, Inc.
SEM-EDS Integration: The Future of Microanalysis!

Micro Photonics, Inc.
Non-Destructive 3D Microscopy Using X-Ray Microtomography

Molecular Imaging
PicoSPM and In Situ Imaging Under Controlled Environments

Olympus America, Inc.
Motorized Microscopy - Making Your Life easier

Polaroid Corp.
Polaroid's Digital Microscope Camera and Simple PCIp Software

Princeton Gamma Tech, Inc.
"What is That PTS I Hear So Much About?"

Q3DM, Inc.
Automating High Resolution Microscopy: Addressing the Challenges
of High Throughput Fluorescence Microscopy for Cell-Based Assays

Quesant Instruments Corp.
Quesant Instruments New Metrology System

Soft Imaging System Corp.
Digital Imaging on Your TEM: Everything You Wanted to Know But
Were Afraid to Ask.


South Bay Technology, Inc.
#1-Sneak Preview of New Techniques and Tool Sets for the D500i
DIMPLER
#2-Ion Beam Sputter Deposition & Etching (IBS/e)
#3-Dedicated Low Energy Ion Milling

Ted Pella, Inc.
New Methods of Microwave-Assisted Processing for Electron
Microscopy

TSL (EDAX booth)
Phase Identification and TEM Tool



See you in Long Beach!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Mon Jul 30 21:16:26 2001



From: Beauregard :      beaurega-at-westol.com
Date: Mon, 30 Jul 2001 21:42:58 -0400
Subject: Re: SEM: ACDsee for 3.11 os (16 bit)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Try site:
http://www.downloadsafari.com/Files/graphprog/A/ACDSee(16-bit).html

A free home viewer that only cost $10 for commercial use is IrfanView.
http://www.irfanview.com/

Paul Beauregard






From daemon Tue Jul 31 02:07:41 2001



From: EM Unit (HISTO) :      emunit-at-mail.wch.sa.gov.au
Date: Tue, 31 Jul 2001 16:26:07 +0930
Subject: Used ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





We are hoping someone out there, preferably within Australia, may have an
ultramicrotome within their facility that has become redundant or obsolete
(preferably an Ultracut E, Ultracut s or a Reichert OMU 3). If so, please
reply ASAP.

Thanks in advance,

Alvis


From daemon Tue Jul 31 07:46:18 2001



From: TCLEEG-at-tsmc.com.tw
Date: Tue, 31 Jul 2001 07:35:19 -0500
Subject: TEM CCD camera selection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

We plan to buy CCD cameras for our two TEMs, JEM-2000 EXII and
JEM2010F. The models on our final (almost) list are
Gatan 692 (1K by 1K) for 2000Ex and 795.201.2 (2K by 2K) for 2010F

We use 2000 EX for routine IC failure analysis and 2010F for HR and
EDS. Both CCDs are quite expensive. We will appreciate any
user experience on both modles or any suggestions on lower cost ones.

Regards,
Tan-Chen Lee


From daemon Tue Jul 31 07:46:19 2001



From: David Pereira :      PereirD-at-war.wyeth.com
Date: Tue, 31 Jul 2001 07:36:07 -0500
Subject: Position Available Research Scientist I

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






You will be responsible for operating a transmission electron
microscope (TEM), scanning electron microscope (SEM), taking and
processing images. Responsibilities include collecting, processing,
embedding, and sectioning tissues in support of electron microscopy
services. You will keep records in compliance with SOP's and GLP's
and maintain laboratory instruments in accordance with SOP's,
troubleshooting equipment problems as needed. You will process
samples in a timely manner according to SOP's. You will maintain and
keep the laboratory clean and organized and ensure that
instrumentation is cleaned, calibrated, and properly maintained
according to facility SOP's. You will ensure that laboratory computer
equipment operates smoothly and work with computer support to
trouble-shoot network and software problems. You may also act as
Pathology monitor for assigned Drug Safety studies. You will tabulate
and report Imaging data to the laboratory supervisor and work with
the laboratory supervisor to ensure the accuracy and completeness of
all Pathology data presented in the final report to the FDA and sent
to archives. You will assist in writing SOP's and appropriate forms
to ensure compliance in all GLP studies and to maintain records for
all studies that are non-GLP or in the spirit of GLP. You may also
assist investigators with (SEM) and digital imaging needs. You will
perform additional departmental and facility support functions such
as (but not limited to) inventory, scheduling, or purchasing.

A Bachelor's degree is required in Biology, Biochemistry, or
Chemistry or a related field with 7 years relevant experience or a
Master's degree with 5 years relevant experience. 5-7 years
experience working in transmission and scanning electron microscopy,
in an industrial, hospital, or highly specialized academic setting is
also required. Candidate should have strong communication and
computer skills.


David W. Pereira
Wyeth-Ayerst Laboratories
641 Ridge Road
Chazy, NY 12921
Tel. (518) 846 6464
Fax. (518) 846 6345
email pereird-at-war.wyeth.com


From daemon Tue Jul 31 08:01:16 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 31 Jul 2001 08:55:22 -0400
Subject: RE: LM- what does Evan's blue stain?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evans Blue will spontaneously react with albumenoids to form a covalent
linkage. Under those circumstances, the dye becomes fluorescent (red).

Evans Blue has been used to tag albumen (1:1). Then it is dialyzed to
remove unbound dye. Finally the tagged albumen is injected into an animal
vascular system. It is used this way to test for sites of extravasation
following injury or as an injection to test for blood volume. It is NOT
known to be hazardous (I believe).

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
Schedule: http://bio.wcupa.edu/cgi-bin/webreserve.acgi
Please call before visiting.


} ----------
} From: Ann P. Ketter
} Sent: Monday, July 30, 2001 8:17 PM
} To: listserver microscopy
} Subject: LM- what does evan's blue stain?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I am working with apoptoic cells in plant tissues. I am using Evan's
} Blue as a test of viability and of apoptosis. Can anyone tell me what
} Evan's blue (EB) stains in the cell? I know that it can not enter a
} cell with an intact membrane but when it enters a damaged or dead cell
} what does the EB actually bind to?
} Ann
}
}
}


From daemon Tue Jul 31 08:08:39 2001



From: David Pereira :      PereirD-at-war.wyeth.com
Date: Tue, 31 Jul 2001 09:02:04 -0400
Subject: Position Available Research Scientist I

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Wyeth-Ayerst Research, the pharmaceutical division of American Home Products, is seeking an experienced and responsible microscopy technician at its Drug Safety facility in Chazy NY. This is a new, state of the art facility located just minutes from Lake Champlain and the Adirondack Mountains. Although located in rural setting it is only an hours drive to Montreal, Quebec and Burlington Vermont.
You will be responsible for operating a transmission electron microscope (TEM), scanning electron microscope (SEM), taking and processing images. Responsibilities include collecting, processing, embedding, and sectioning tissues in support of electron microscopy services. You will keep records in compliance with SOP's and GLP's and maintain laboratory instruments in accordance with SOP's, troubleshooting equipment problems as needed. You will process samples in a timely manner according to SOP's. You will maintain and keep the laboratory clean and organized and ensure that instrumentation is cleaned, calibrated, and properly maintained according to facility SOP's. You will ensure that laboratory computer equipment operates smoothly and work with computer support to trouble-shoot network and software problems. You may also act as Pathology monitor for assigned Drug Safety studies. You will tabulate and report Imaging data to the laboratory supervisor and work with the laboratory supervisor to ensure the accuracy and completeness of all Pathology data presented in the final report to the FDA and sent to archives. You will assist in writing SOP's and appropriate forms to ensure compliance in all GLP studies and to maintain records for all studies that are non-GLP or in the spirit of GLP. You may also assist investigators with (SEM) and digital imaging needs. You will perform additional departmental and facility support functions such as (but not limited to) inventory, scheduling, or purchasing.

A Bachelor's degree is required in Biology, Biochemistry, or Chemistry or a related field with 7 years relevant experience or a Master's degree with 5 years relevant experience. 5-7 years experience working in transmission and scanning electron microscopy, in an industrial, hospital, or highly specialized academic setting is also required. Candidate should have strong communication and computer skills.
Interested individuals should send their resumes, including a description of their expertise and references to:

David W. Pereira
Wyeth-Ayerst Laboratories
641 Ridge Road
Chazy, NY 12921
Tel. (518) 846 6464
Fax. (518) 846 6345
email pereird-at-war.wyeth.com



From daemon Tue Jul 31 08:34:52 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 31 Jul 2001 09:23:55 -0400
Subject: Re: Preparation of Araldite sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 2:24 PM -0400 7/30/01, Don Gantz wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

**************
Hi Donald,

Much would depend on the formulation of araldite used. Like Epon, et
al, it can be harder or softer if the ratios of components is varied.
My experience is that, keeping relative hardness in mind, it behaves
pretty much like epon. I'm sure others, with more experience working
with it will have more info on this.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Jul 31 08:47:57 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 31 Jul 2001 23:44:00 +1000
Subject: RE: Replacement Vacuum Pumps - Need Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I expect that no two vacuum engineers would agree on the ideal pump size for a
particular system: to wit the tiny pumps used by JEOL and the huge twin pumps
used on most Hitachi scopes.
Mechanical (also called rotary and fore pumps) are purchased largely on three
figures: ultimate vacuum, pumping speed / volume (expressed as a volume over a
time) and price.
A small pump is cheaper to buy and uses less electricity, they tend to be
quieter running (depending on make), larger pumps are the converse in
properties but they pump down faster especially with new film in a TEM and when
evacuating a larger space rather then a small airlock.
In either case you must opt for a two-stage pump. Direct drive types are less
trouble since belts break more frequently than couplings. Couplings become
noisy and so warn the user that they need attention, but belts break with no
warning. (It pays to inspect belts every three months or so, cracks on the
inside of a belt indicated that a replacement is needed). Belt driven pumps run
a little slower and live a little longer.
Having said that, I would buy a direct drive pump one size larger than the
reasonable minimum required. I think that 4cubic meters/ hour is a reasonable
minimum for most EMs and so a 6 might be a good size. A 2 would work, but
encourage an awful lot of long coffee breaks and a ten is a bit of overkill. I
don't know how the Hitachi twin pumps share the work load, but it may not be
easy to combine the pumping circuits, so you may need to retain two somewhat
smaller pumps (2x 3 or 4 m3/h).
Over the years I have used pumps of numerous makes and while they all work and
most are quiet reliable, I would get a Leybold or an Edwards because they
seemed to run quietest and achieved, for a given size pump the best ultimate
vacuum. But these things may have changed in recent times. Perhaps other
listers have an opinions on pumps performance.
However, I should also declare my bias: ProSciTech is an agent (in Australasia)
for Edwards pumps. Happily I have predilections for Edwards.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, July 31, 2001 5:42 AM, SEM Machine [SMTP:SEM-at-ACATC.AME.Arizona.edu]
wrote:
}
}
} We have a Hitachi S-650 with two 160VP pumps that need to be replaced. I
} have called Hitachi about information on the pumps, but they are having some
} difficulty in locating any specs on them. Searching the Internet did not
} give any information either.
}
} The pumps plug into what appears to be a standard 3-prong socket and the
} pumps have information on the housing for 110 V, so I'm thinking that the
} power requirements are fairly standard. What I don't know is what flow
} rate, et cetera, to get for the replacement pumps.
}
} Does anyone know where I could locate the pump specs or could you recommend
} some pumps that would work? And, in case you haven't figured it out
} already, I'm not an expert on vacuum systems, so I may be going about this
} the wrong way by looking at specs that really don't matter. If someone
} could let me know what parameters are the important things to look for, I'd
} be grateful.
}
} Thanks,
}
} -Norman Kay
} AME Dept.
} The Univ. of Arizona
}
}
}
}



From daemon Tue Jul 31 09:09:41 2001



From: Jeff Turmelle :      jefft-at-ewing.ldgo.columbia.edu
Date: Tue, 31 Jul 2001 06:31:05 -0500 (GMT)
Subject: please remove micro@ewing.ldeo.columbia.edu from your mailing list

Contents Retrieved from Microscopy Listserver Archives
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There is no micro user onboard our research ship, and you are inundating our
inmarsat connection with your email list. Please take care of this as soon as
possible please,

Thank you,

Jeff Turmelle
Sys Admin, R/V Maurice Ewing
Lamon-Doherty Earth Observatory of Columbia University
jefft-at-ldeo.columbia.edu



From daemon Tue Jul 31 10:08:34 2001



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 31 Jul 2001 09:58:31 -0500
Subject: Kodak MDS-100s on eBay?

Contents Retrieved from Microscopy Listserver Archives
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The Kodak MDS-100 microscope camera system has been showing
up on eBay lately... is it discontinued and being liquidated?

They normally sold for $500-1000, but it looks like these
will go for considerably less... see the search link below.

- John

http://search-desc.ebay.com/search/search.dll?MfcISAPICommand=GetResult&pb=&ht=1&st=2&query=mds+100+microscope&SortProperty=MetaEndSort&srchdesc=y



From daemon Tue Jul 31 11:18:25 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 31 Jul 2001 12:11:05 -0400
Subject: Re: Ask-A-Microscopist: Focus definitions needed

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Question: I want to know the exact meaning of terms
defocus,underfocus,focus,infocus and overfocus how
they are classified for a particular object.

I would appreciate receiving reply in this regard

Dear Mullapudi Srinvas,
For an ideal lens, all the electrons leaving a point on a specimen
will be brought together in a point in a plane--the focal plane. When the
lens currents are adjusted so that this plane is coincident with the
detection system (film, CCD, etc.), the image will be in exact focus.
(N.b., focus is a property of the image and is the same regardless of the
particular object, although different points in thick objects can have
different focal planes.) The distance between the focal plane and the
detector plane is the amount of defocus--positive for overfocus (focal
plane above the detector plane) and negative for underfocus. If you look
at how information from the specimen is conveyed to the image as a function
of spatial frequency--i.e., how the large and small features appear--you
will find that there is a curve which defines the relationship between the
amplitude of the wave of electrons leaving the specimen and that of the
wave incident on the detector. This curve is the contrast transfer
function (CTF), and it is of the same sign for frequencies lower than a
certain value, then it changes sign and oscillates at higher frequencies.
The amount of defocus which produces a CTF extending to the largest
frequency before it changes sign gives the condition called Schertzer (sp?)
focus. This condition results in images in which easily interpretable
information extends to the highest resolution possible. Images are often
taken somewhat underfocus, since that condition causes interference fringes
to occur at edges due to the sign reversal of the CTF and enhances their
visibility. This is often useful for images of biological specimens. I
hope this answers your questions.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Tue Jul 31 12:51:46 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 31 Jul 2001 12:43:33 -0500
Subject: Older SEM

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The Microscopy Lab at KU is getting a new SEM at the beginning of October.
Consequently our current SEM, a Hitachi S-570 needs to be moved out before that
time. If anyone needs an older SEM please contact me.
Thank you
Bruce
Bruce Cutler, Ph.D.
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas





From daemon Tue Jul 31 13:34:42 2001



From: May, Dan :      DMay-at-iti-med.com
Date: Tue, 31 Jul 2001 14:04:54 -0500
Subject: SEM Services in Minneapolis/St.Paul Minnesota Area

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Microscopy-at-sparc5.microscopy.com


Lee,

There is a substance called choline hydroxide (Sigma Aldrich) that has
10,000:1 selectivity (Si:SiO2). Dilute 25 ml. choline with 65 ml of DI
water at 90 degrees C (must be maintained accurately). Depending on the
thickness of your poly, it should take 1 - 5 minutes to remove the poly and
keep the GOX intact. Hope this helps. Hey, if you know Yih-Shung Lin and
he is still there at TSMC tell him that John Staman said "Hi".

Regards,

John Staman, LSI Logic

-----Original Message-----
} From: "TCLEEG-at-tsmc.com.tw"-at-sparc5.microscopy.com
[mailto:"TCLEEG-at-tsmc.com.tw"-at-sparc5.microscopy.com]
Sent: Tuesday, July 31, 2001 6:35 AM
To: Microscopy-at-sparc5.microscopy.com





I am in search of SEM services for small metallic medical products in the
Minneapolis or St. Paul area in Minnesota. If there are any suggestions, I
would appreciate them.
Thank you.
Dan May
IntraTherapeutics
dmay-at-iti-med.com {mailto:dmay-at-iti-med.com}



From daemon Tue Jul 31 15:17:53 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 31 Jul 2001 13:09:46 -0700
Subject: Re: Replacement Vacuum Pumps - Need Help

Contents Retrieved from Microscopy Listserver Archives
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Dear Norman,
The Cutevac 160VP is a 160 l/min direct-drive rotary pump. It plugs into any
110v socket. Any direct-drive single or double stage vacuum pump with the
same capacity should work.
BTW, what are you doing with the old pumps? I have seven of them running in
my lab and the oldest have been running continuously for 19.5 years. I love
them, fix them myself when they leak oil and would love to get some more.
At 12:41 PM 7/30/01 -0700, you wrote:
}
} We have a Hitachi S-650 with two 160VP pumps that need to be replaced. I
} have called Hitachi about information on the pumps, but they are having some
} difficulty in locating any specs on them. Searching the Internet did not
} give any information either.
}
} The pumps plug into what appears to be a standard 3-prong socket and the
} pumps have information on the housing for 110 V, so I'm thinking that the
} power requirements are fairly standard. What I don't know is what flow
} rate, et cetera, to get for the replacement pumps.
}
} Does anyone know where I could locate the pump specs or could you recommend
} some pumps that would work? And, in case you haven't figured it out
} already, I'm not an expert on vacuum systems, so I may be going about this
} the wrong way by looking at specs that really don't matter. If someone
} could let me know what parameters are the important things to look for, I'd
} be grateful.
}
} Thanks,
}
} -Norman Kay
} AME Dept.
} The Univ. of Arizona
}
Regards adn good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Jul 31 15:40:19 2001



From: Jenichen :      jenichen-at-proscan.de
Date: Tue, 31 Jul 2001 22:35:23 +0200
Subject: Re: TEM CCD camera selection

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"TCLEEG-at-tsmc.com.tw"-at-sparc5.microscopy.com schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi
}
} We plan to buy CCD cameras for our two TEMs, JEM-2000 EXII and
} JEM2010F. The models on our final (almost) list are
} Gatan 692 (1K by 1K) for 2000Ex and 795.201.2 (2K by 2K) for 2010F
}
} We use 2000 EX for routine IC failure analysis and 2010F for HR and
} EDS. Both CCDs are quite expensive. We will appreciate any
} user experience on both modles or any suggestions on lower cost ones.
}
} Regards,
} Tan-Chen Lee

Dear Tan Chen Lee!

It would be useful to contact You directly to avoid direct advertisement on
this mail list. Please send
Your e-mail adress to the adress specified below.

F.W. Jenichen
Proscan GmbH, Germany
e-mail: fwj-at-proscan.de
net: http://www.proscan.de




From daemon Tue Jul 31 16:55:36 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 1 Aug 2001 09:48:10 GMT+1200
Subject: belt vs direct drive pumps

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Just to chip in:

My experience is that belt-drive pumps last a lot longer than direct,
I have a couple of JEOL ones that have run more-or-less continuously
for almost 30 years, with annual oil changes, frequent belt
inspection and replacement as Jim says, and one replacement of the
vane springs! Direct-drive ones don't seem to be able to do anything
like that.

They are also quieter, particularly if the belts are sprayed
occasionally with belt dressing.

Gimme belt-drives every time.

cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Jul 31 17:48:49 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 31 Jul 2001 15:43:09 -0700
Subject: Re: Sucrose Gradient Centrifugation

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I don't remember the procedure, but you may remove PERCOLL after
purification by centrifugation at some particular acceleration (g). It's
clearly stated in the PERCOLL Instruction Manual. If you need real data, I
may check. I was using negatively stained mitochondria for TEM after
PERCOLL purification and did not noticed any PERCOLL particles in the
preparation, so it's not a problem if you remove PERCOLL properly after
purification.

Sergey.

At 06:54 PM 7/26/01 -0600, you wrote:
} I have a question about using Percoll for TEM - someone told me just last
} week that you shouldn't use Percoll if you'll be doing thin sections,
} since it is a silica (can damage knives) - has anyone used it and either
} seen or not seen gunk in their sections? Scratches &/or damaged a knife?
} Sea creatures or other beach denizens? (Sorry, I'm tired and couldn't
} resist)
}
} I'm not planning to use it any time soon, but hearing about it again makes
} me curious about using it to prep for EM. I've seen nasty sucrose crystals
} a few times with samples from sucrose gradients, but they are mostly an
} annoyance.
}
} Thanks!
}
} Tamara
}
}
} On Thu, 26 Jul 2001, Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It's better to use PERCOLL solution. It's isoosmotic and you could
} } separate cells from microsomal fraction or viruses etc. You may also
} } separate different cell types by this technique. PERCOLL was manufactured
} } by Pharmacia, which is a part of Amersham now, I believe. Good
} luck. Sergey
} }
} } At 11:06 AM 7/26/01 -0700, you wrote:
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Jul 31 18:13:39 2001



From: SEM Machine :      SEM-at-ACATC.AME.Arizona.edu
Date: Tue, 31 Jul 2001 16:09:54 -0700
Subject: Replacement Vacuum Pumps - Follow Up

Contents Retrieved from Microscopy Listserver Archives
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Many thanks to everyone who replied. I now have some idea of where I need
to go from here.

To answer a few of the questions that came up, the problem was that before
the SEM was donated to us, I believe it was not serviced for a while nor was
it handled very gently during transit. I just started working with the unit
and recently found out that when it was delivered, it was left at the wrong
building & sat on the loading dock for a few days - during monsoon season.
So, needless to say, this unit has had a rough life. We've had it serviced
& the Hitachi tech said that the pumps would most likely need to be replaced
soon, but I should have questioned more into why she thought that. The pump
failed a little over 3 months after the service, so I guess she was on to
something. I'm also learning as I go, since there isn't anyone here to
teach me, which I'm sure has contributed to the early demise of the pump.

What happened to the pump was that one morning when I came in, there was a
puddle of oil around the pump, so something obviously gave out during the
night (all those years of schooling have taught me a thing or two...). Of
the two pumps, the one that died was the "stronger" one, since we already
knew that the other pump was a bit weaker. I called Hitachi & they told me
that they didn't have a rebuild kit, per se, but that they could send a list
of parts - no guarantees on if they were still available, though. Based on
my past experience with trying to find parts for our SEM, I'm pretty sure
very few of the parts are still available. I did give the pump to our
machine shop, which has done a bit of work on vacuum pumps, and they rebuilt
it as best they could, but it wasn't enough, since I cannot get a high
vacuum now.

I should mention, though, that a Hitachi tech did contact me after I
submitted the initial post & was very helpful on getting me some information
on pumps that could replace our unit. Based on what he said, and also from
replies here, it appears that we need something that can do about 9 cfm. I
also plan on contacting a few of the companies suggested here on costs to
rebuild the pumps, since they may be salvageable.

Thanks again,

-Norman





From daemon Tue Jul 31 18:36:17 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 31 Jul 2001 16:38:18 -0800
Subject: staining hyphae for LRWhite

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Hi Everyone

I am trying to stain some formaldehyde preserved (no osmium) fungal
hyphae so I can visualize them after embedding in LRWhite.

Any suggestions? Tryphan blue works until you transfer the hyphae to
LRWhite, which extracts all color. I have oriented the hyphae in
small blocks of agar, but the small size of the hyphae makes it
difficult to see them if I stain the agar, say with Sudan black as
was once suggested years ago.

I hope to hear from someone in this regard

Steve Barlow


From daemon Tue Jul 31 19:09:51 2001



From: macke-at-bgnet.bgsu.edu
Date: Tue, 31 Jul 2001 19:06:27 -0500
Subject: TEM Gatan Model 600 Ion Mill

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We have purchased Gatan Model 600 Dual Ion Mill for use in the
preparation of foils of geological material for TEM applications.
Since this unit was purchased used from a government lab it was
delivered without technical support and with incomplete
documentation, leaving many unanswered questions. We are having
problems maintaining high vacuum (the diffusion pump shuts off) and
cannot find the source of the problem. Help from anyone familiar
with this device would be greatly appreciated.

We are attempting to thin quartz arenites and have spent the majority
of our time (when the unit is operational) in an effort to optimize
the thinning process. Any input in that regard would also be welcome.

Thanks in advance for any information you can provide.

Kevin Macke
Bowling Green State University
Department of Geology
Bowling Green, OH
Email: macke-at-bgnet.bgsu.edu


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp


From daemon Tue Jul 31 19:37:10 2001



From: dlongerbeam-at-captusnetworks.com ()
Date: Tue, 31 Jul 2001 19:32:04 -0500
Subject: Ask-A-Microscopist:Optical Scope Purchase

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dlongerbeam-at-captusnetworks.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
July 31, 2001 at 19:24:09
---------------------------------------------------------------------------

Email: dlongerbeam-at-captusnetworks.com
Name: Donald Longerbeam

Organization: none

Education: Graduate College

Location: Roseville Ca.

Question: I was trained as a molecular biologist and did work with
various microscopes over the years. However I have been out of the
field for 25 years. I now have a son who I wish to introduce to the
microscopic world. Who is making a good and reasonable microscope at
this time. Olympus and Zeiss are certainly good - but they are too
expensive. I am willing to spend up to $1000 apiece for a good
binocular dissecting microscope and a good general higher power scope
- . Thanks.
Donald Longerbeam.

---------------------------------------------------------------------------


From daemon Tue Jul 31 21:59:09 2001



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Tue, 31 Jul 2001 22:52:41 -0400
Subject: twosome needed for M&M golf tourney

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My usual golf partners won't be able to make it to M&M this year. Brian
Cook and I are looking for two others to pair up with.

Thanks,
Lucille Giannuzzi

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 882-1500
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Wed Aug 1 03:52:16 2001



From: S.C.Hogg :      S.C.Hogg-at-sheffield.ac.uk
Date: Wed, 1 Aug 2001 09:42:34 +0100
Subject: Hot stage Hi-Vac SEM

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Hello,

We've been doing some hot stage environmental SEM (ESEM)
work with aluminium-silicon alloys (up to 650°C), and we get some
interesting contrast enhancement features as the temperature is
increased. We are trying to discount various parameters and it
seems that hot stage high vacuum (conventional) SEM would help
determine if the effect has something to do with the gas in the
chamber.

Will a conventional Everhart-Thornley (E-T) detector be able to
magange this, or will contamination and out-gassing be a problem?
Also I'm aware that unlike the gaseous secondary electron detector
(GSED) in the ESEM, the E-T detector is sensitive to light, will this
be a problem too? If it does not work, will it simply not give a good
image, or is it likely to damage the dectector?
Finally if anyone has tried this technique and it worked, any tips for
good imaging?

Thank-you,

Simon Hogg



--

Dr. Simon Hogg
Thixoforming Research Group
University of Sheffield
Department of Engineering Materials
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
Tel. 0114 222 5934
Fax. 0114 222 5943


From daemon Wed Aug 1 06:12:00 2001



From: Per =?iso-8859-1?Q?H=F6rstedt?= :      per.horstedt-at-pathol.umu.se
Date: Wed, 01 Aug 2001 13:04:52 +0200
Subject: Re - twosome needed for M&M golf tourney

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Where's the connectioln between this list and golf partner problems.
Hard to see for me in Sweden.
There should be other channels for personal stuff like this.

Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

per.horstedt-at-pathol.umu.se
phone int-46-90-7851541
fax int-46-90-7851215



From daemon Wed Aug 1 06:59:00 2001



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Wed, 01 Aug 2001 06:59:21 -0500
Subject: Re: SEM Services in Minneapolis/St.Paul Minnesota Area

Contents Retrieved from Microscopy Listserver Archives
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Hi Dan,
The Imaging Center in the College of Biological Sciences houses a new
Hitachi S3500N SEM. The instrument is a variable pressure system equipped
as follows:

*** Hitachi S-3500N, variable pressure SEM ***

- Standard stage with motor driven X & Y axes.

- Robinson Backscattered Electron Detector (BSE).

- Hitachi Hi-Mouse software.

- Infrared ChamberScope.

- Photo CRT Unit, recording camera.

- Ethernet interface.

*** EMITECH K-1150 CRYOGENIC SYSTEM ***
- http://www.emitech.demon.co.uk/

*** EDAX Phoenix X-Ray Microanalysis System ***

- Windows NT Operating System

- Super Ultra-Thin Window 10mm LN-cooled Detector

- EDX Fast Mapping, EDX Quant Mapping, EDX Line Scan, Spectral Mapping/Data
Recovery, SEM Quant Package.

We also house a large assortment of TEM and LM instrumentation and
preparation equipment. We can provide service or teach you how to use the
instrument. Feel free to check out our web site and contact either Mark
Sanders (msanders-at-biosci.umn.edu) or Gib Ahlstrand
(giba-at-puccini.crl.umn.edu) with any questions or to arrange a demonstration.
Cheers,
Mark
****************************************************
Mark A. Sanders University of Minnesota
Program Director Twin Cities Campus
Imaging Center St. Paul, MN 55108
23-35 Snyder Hall ph: 612-624-3454
1475 Gortner Ave. fax: 612-624-1799
http://biosci.umn.edu/imagingcenter


} From: "May, Dan " {DMay-at-iti-med.com}
} Date: Tue, 31 Jul 2001 14:04:54 -0500
} To: "'Microscopy-at-MSA.Microscopy.com'" {Microscopy-at-sparc5.microscopy.com}
} Subject: SEM Services in Minneapolis/St.Paul Minnesota Area
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am in search of SEM services for small metallic medical products in the
} Minneapolis or St. Paul area in Minnesota. If there are any suggestions, I
} would appreciate them.
} Thank you.
} Dan May
} IntraTherapeutics
} dmay-at-iti-med.com {mailto:dmay-at-iti-med.com}
}
}
}



From daemon Wed Aug 1 08:25:09 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Wed, 01 Aug 2001 09:11:15 -0400
Subject: plastic embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists:

I am looking for a suitable plastic embedding material which should have the following characteristics:

- applicable for light microscopy
- not be autofluorescent
- used for immunocytochemistry, i.e. tissue should retain immunogenicity
- soft enough to cut 20 micron or thicker sections easily
- ability to embed large pieces e.g. entire rat lung
- opacity to allow identification of parts of embedded tissue (this excludes paraffin)

I hope I am being realistic and there is a product out there to meet these criteria. This group has never let me down.

Thank you in advance.
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Wed Aug 1 08:44:01 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 1 Aug 2001 09:39:44 -0400
Subject: Re - twosome needed for M&M golf tourney

Contents Retrieved from Microscopy Listserver Archives
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Relax,
There has been a precedent in the past for using this forum for arranging social events at the M&M meeting. There are no other forums for reaching the large number of participants that go to the meeting. It has never gotten out of hand before and someone shouldn't be criticized for doing it. It is at social functions that sometimes the most valuable scientific work at a conference gets done.

If you do attend one of the M&M meetings, look me up; I'll buy you a beer.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Per Hörstedt [mailto:per.horstedt-at-pathol.umu.se]
Sent: Wednesday, August 01, 2001 7:05 AM
To: microscopy-at-sparc5.microscopy.com


Where's the connectioln between this list and golf partner problems.
Hard to see for me in Sweden.
There should be other channels for personal stuff like this.

Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

per.horstedt-at-pathol.umu.se
phone int-46-90-7851541
fax int-46-90-7851215



From daemon Wed Aug 1 10:02:58 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Tuesday, July 31, 2001 2:34 PM
Subject: Re: Replacement Vacuum Pumps - Need Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A quick note about replacing rotary pumps on Hitachi Microscopes. The
microscope provides 100 VAC (Japan Voltage) for operation. Hitachi
Instruments service uses Edwards pumps as replacements in the USA, and they
seem to be OK at this low voltage. Other brands must be checked.

Dunniway Stockroom doesn't like to try to rebuild the Hitachi brand pumps
because they find it hard to get parts. So they don't deal with them. Mary
must know a secret about parts for rebuilding them.

Direct drive pumps operate at higher RPM and wear out sooner than belt drive
pumps.

Ronald Vane
XEI Scientific
Redwood City, CA

-----Original Message-----
} From: Mary Mager {mager-at-interchange.ubc.ca}
To: SEM Machine {SEM-at-ACATC.AME.Arizona.edu}
Cc: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Wed Aug 1 10:55:52 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 1 Aug 2001 10:53:27 -0500 (CDT)
Subject: POSTDOCTORAL POSITION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL POSITION
in Quantitative Electron Microscopy

A postdoctoral position is available in the area of quantitative TEM/TED
of materials starting September 1, 2001. The research project focuses
on developing quantitative methods of solving structures from diffraction
patterns and HREM images. A good background in computer programming in
fortran or C is required, as well as an understanding of dynamical
diffraction theory. Prior experience in HREM or Direct Methods would be
an advantage.

For further information or applications email Professor L. D. Marks
at ldm3-at-risc4.numis.nwu.edu


-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html




From daemon Wed Aug 1 12:13:59 2001



From: Young, Courtney :      CYoung-at-mtech.edu
Date: Wed, 1 Aug 2001 11:06:48 -0600
Subject: Inquiry for used SEM/EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Montana Tech's Dept of Metallurgical and Materials Engineering is in need of
replacing its SEM/EDX system and is looking for an economical used system.
An SEM w/o EDX can be considered since the EDX on our current system can
likely be attached. Parties interested in divesting their SEM/EDX system
should contact Dr. Courtney Young by e-mail as soon as possible at
cyoung-at-mtech.edu or by phone at 406-496-4158 to let him know what the system
is, what its condition is, its age, if there is an EDX attached, and what
the cost will be to get it delivered to Montana Tech in Butte MT.


From daemon Wed Aug 1 12:54:39 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 1 Aug 2001 12:48:10 -0500
Subject: Metamorph vs ImagePro & networks

Contents Retrieved from Microscopy Listserver Archives
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We are long time users of the Metamorph image analysis software
package from Universal imaging. Our sales rep had told us they were
coming up with a networkable edition so that we could buy a licence
for a number of copies that would allow users on our campus to
install the software on their own lab computers and it would run as
long as the number of copies running at any moment was not more than
the number of licences we had. It would check this over the internet
backbone. We are now told that they have either abandoned this idea
or never considered it depending on who and when we talk to them. It
is my understanding that ImagePro does have such a capability and we
are considering switching over to it. Is there any one out there
with experience with this set up? Does it work? I am particularly
interested in a solution in which the software runs on the local
computer and not on a server so that we would not have to waste time
transferring files, etc. over the net. I assume it would be a lot
faster to have the software simply look for the licence over the net.
If i am wrong about this, I would be happy to be educated on this
matter also.

I would also welcome comments comparing the ease and versatility of
ImagePro vs. Metamorph.

Thanks, Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Aug 1 12:54:40 2001



From: Steve Beck :      becks-at-sunynassau.edu
Date: Wed, 01 Aug 2001 13:52:56 -0400
Subject: Fall 2001 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
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Fall 2001 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section E2)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A fourteen week, Fall 2001 semester, course in Biological Transmission
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered one evening per week
(Thursdays) starting at 5:30 PM. Classes will begin on September 6 and end
on December 20, 2001.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$92 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Wed Aug 1 13:19:43 2001



From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 1 Aug 2001 13:14:42 -0500
Subject: Re: MSA Surplus Equipment pages :Inquiry for used SEM/EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you looked at the MSA Surplus Equipment pages

http://www.msa.microscopy.com/SurplusEquipment/

Nestor
Your Friendly Neighborhood SysOp


}
}
} Montana Tech's Dept of Metallurgical and Materials Engineering is in need of
} replacing its SEM/EDX system and is looking for an economical used system.
} An SEM w/o EDX can be considered since the EDX on our current system can
} likely be attached. Parties interested in divesting their SEM/EDX system
} should contact Dr. Courtney Young by e-mail as soon as possible at
} cyoung-at-mtech.edu or by phone at 406-496-4158 to let him know what the system
} is, what its condition is, its age, if there is an EDX attached, and what
} the cost will be to get it delivered to Montana Tech in Butte MT.

--
===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4289
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From daemon Wed Aug 1 13:33:16 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 1 Aug 2001 13:28:47 -0500
Subject: Computers: PC disks in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Recently, we started experiencing a problem when using PC disks
(1.8MB and Zips) in Macs. We are able to access and write to the PC
disks but can not eject the disks without rebooting the Mac. We get a
message indicating that the file is in use (even though we have quit
the program) and a second message indicating that file sharing could
not be enabled. I suspect that this may be due to the disks being
formatted on the latest PC operating system. Does anyone know of a
fix? Thanks.

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Aug 1 13:52:56 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 1 Aug 2001 14:48:34 -0400
Subject: Computers: PC disks in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
Use Mac formatted disks on the PC and use Conversions Plus from DataViz. I have never had a problem doing anything this way. What works best is to use PC Formatted Zips and then quick format them in the PC to the Mac Format. I have been doing this for about 4 years and it works great. I never lose the long formatted names going to either platform.

The one thing that you do have to take care of is to make sure that you close the directory structure on the Mac before you eject it and carry it to the PC. If you modify it on the PC after you ejected it on the Mac with the directory structure open and then go back to the Mac, your information can get lost. (I think that I got that sequence right --I may have gotten it backwards.) Whatever you do, close the file structure before ejecting the disk from the Mac.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Wednesday, August 01, 2001 2:29 PM
To: Microscopy-at-sparc5.microscopy.com


Recently, we started experiencing a problem when using PC disks
(1.8MB and Zips) in Macs. We are able to access and write to the PC
disks but can not eject the disks without rebooting the Mac. We get a
message indicating that the file is in use (even though we have quit
the program) and a second message indicating that file sharing could
not be enabled. I suspect that this may be due to the disks being
formatted on the latest PC operating system. Does anyone know of a
fix? Thanks.

John B.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Aug 1 14:21:49 2001



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 1 Aug 2001 14:16:46 -0500
Subject: Mounting/Embedding Epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,

I am looking for a suitable mounting material to mount small fossil bone
samples. The sample extraction method requires the material to be optically
clear and will allow some of the mounting to be mixed with the sample. So
it would be desirable for the mounting material not to interfere with the
elemental isotopic ratio that exists in the sample. Phosphorus compounds are
of most concern. Any suggestions would be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Wed Aug 1 14:40:10 2001



From: ed_bachmann-at-unc.edu (Ed Bachmann)
Date: Wed, 1 Aug 2001 15:33:44 -0400 (Eastern Daylight Time)
Subject: Optical - Zeiss Standard 25 - opinions, value?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering the purchase of a used Zeiss Standard 25 over the web. I will
not have the opportunity to inspect the scope and can judge it from photographs
only. It has 5 plan acro objectives (2.5, 10, 25, 40, and 100 (oil)), but
otherwise seems to be the "base" configuration with no optional components.

I have searched the web for information on this scope and have come up
empty-handed (empty-screened?)
I would like to know when they were produced, where they fell in the Zeiss
product line, what the "successor" to the Standard 25 was or is, where I might
find an instruction manual or old product catalog that describes optional
configurations, and any other information about the scope.

Would anyone venture an opinion on the quality of this scope or its value? The
asking price is $1500. It is said to be in good condition.

Thanks very much,
Ed Bachmann

Ed Bachmann
Odum Institute for Research in Social Science
Manning Hall CB 3355
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-3355
(919) 962-0512



From daemon Wed Aug 1 15:26:04 2001



From: Rodney McCabe :      rmccabe-at-lanl.gov
Date: Wed, 01 Aug 2001 14:19:00 -0600
Subject: Re: Computers: PC disks in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John

We have had an identical problem with PC formatted Jaz and Zip disks. It
appears, in our case, there is a conflict with Norton Anti-virus, Apple's
PC Exchange, and PC-formatted Iomega media. If we disable Norton
Anti-virus Auto Protect before inserting the Zip or Jaz disk, the problem
goes away. This is obviously not a permanent solution. Let me know if you
come up with a better solution.

Rod

At 01:28 PM 8/1/01 -0500, John J. Bozzola wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

-----------------------------------------------------------------------------------------
Rodney McCabe
MST-8 : Structure Property Relations
MS-G755
Los Alamos National Laboratory
Los Alamos, NM 87545
Phone: (505) 665-3289
-----------------------------------------------------------------------------------------



From daemon Wed Aug 1 17:26:45 2001



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Wed, 01 Aug 2001 15:21:59 -0700
Subject: Cambridge S-250 SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi Everyone,

A nicely mothballed Cambridge S-250 with many lovely accessories has
fallen into my lap. I am contemplating bringing it back from the dead.

Is there anyone among you who is still delighted their S-250 who would
encourage me to proceed?

Bart Cannon
Cannon Microprobe
Seattle



From daemon Wed Aug 1 17:49:32 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 1 Aug 2001 18:44:46 -0400 (EDT)
Subject: to MSA attendees

Contents Retrieved from Microscopy Listserver Archives
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Dear folks,

I have read with fascination Debbie¹s description of the MSA Facility
Workshop, and it sounds great. However, for those of you involved in
disease and pathology microscopy, the session on Emerging Diseases
(unfortunately on the same day) will also pique your interest. We have
internationally recognized speakers, including one from Australia and two
from the Centers for Disease Control and Prevention. Also there are an
AIDS and opportunistic pathogen expert from Geo Wash U and an
immunopathologist and transplantation expert from Duke. There are talks
on Ebola virus, use of EM to detect new viruses, prion diseases, agents
of biowarfare, and others. I hope many of you can join us.


Sara




Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Aug 1 18:50:12 2001



From: Nancy Robertson :      pfnlr-at-UAA.ALASKA.EDU
Date: Wed, 01 Aug 2001 15:48:20 -0800
Subject: compound microscope for stained thick sections

Contents Retrieved from Microscopy Listserver Archives
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I need to order a compound microscope to look at thick sections from
plastic embedded plant material before thin sectioning. Could someone
suggest one that cost less than $2,500.00?

Thanks, Nancy


From daemon Wed Aug 1 19:32:31 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 2 Aug 2001 10:29:27 +1000
Subject: RE: Hot stage Hi-Vac SEM

Contents Retrieved from Microscopy Listserver Archives
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It seems that you already know the answer: If there is any light emitted, and
at 650 C most materials would, then the photomultiplier tube, which is integral
to the E-T detector would be overloaded. You can check that by increasing the
multiplier tube amplifier from zero up, until it overloads or you reach your
usual mid-range setting.
Similarly, outgasing at high temperatures is a problem and I would be surprized
if you could attain a vacuum sufficient for the ET detector - BS detectors are
rather less sensitive to poor vacuum.
One obvious way to minimize outgasing would be to have a very small area
heated.
My question is: How do you avoid heat damage within the chamber, including to
the plastic (could be quartz) scintillator and light pipe?
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, August 01, 2001 6:43 PM, S.C.Hogg [SMTP:S.C.Hogg-at-sheffield.ac.uk]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
}
} We've been doing some hot stage environmental SEM (ESEM)
} work with aluminium-silicon alloys (up to 650?C), and we get some
} interesting contrast enhancement features as the temperature is
} increased. We are trying to discount various parameters and it
} seems that hot stage high vacuum (conventional) SEM would help
} determine if the effect has something to do with the gas in the
} chamber.
}
} Will a conventional Everhart-Thornley (E-T) detector be able to
} magange this, or will contamination and out-gassing be a problem?
} Also I'm aware that unlike the gaseous secondary electron detector
} (GSED) in the ESEM, the E-T detector is sensitive to light, will this
} be a problem too? If it does not work, will it simply not give a good
} image, or is it likely to damage the dectector?
} Finally if anyone has tried this technique and it worked, any tips for
} good imaging?
}
} Thank-you,
}
} Simon Hogg
}
}
}
} --
}
} Dr. Simon Hogg
} Thixoforming Research Group
} University of Sheffield
} Department of Engineering Materials
} Sir Robert Hadfield Building
} Mappin Street
} Sheffield
} S1 3JD
} Tel. 0114 222 5934
} Fax. 0114 222 5943


From daemon Wed Aug 1 20:02:15 2001



From: Boron nitride :      boronnitride-at-hotmail.com
Date: Wed, 01 Aug 2001 18:56:20 -0600
Subject: EELS Spectra of cubic BCN phase

Contents Retrieved from Microscopy Listserver Archives
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Hello. everybody,

I acquired some EELS spectrum of diamond-like BCN phase and would like to
analyze them. Does anyboday know if a standard spectrum of cubic BCN
(theoretical or experimental) can be found somewhere?

Thanks in advance for your help!


James


_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp



From daemon Wed Aug 1 21:38:21 2001



From: Kevin Macke :      kevinmacke2-at-hotmail.com
Date: Wed, 1 Aug 2001 21:31:55 -0500
Subject: TEM Gatan Ion Mill update

Contents Retrieved from Microscopy Listserver Archives
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Thank you to all who responded to my previous posting. Like most of
the respondents, we thought the most likely problems were a faulty
thermal switch or a lack of cooling water, so we checked those first
(I forgot to include that detail. sorry). We managed to trace the
problem down to the thermocouple gauge today. Apparently the optical
switch that triggers the 20-amp relay after the preset vacuum is
reached is malfunctioning. After discussing the problem with the
people at Gatan and discovering the cost of a new gauge
(approximately $700-1500, depending on the source), we went the low
buck route and bypassed the TC gauge by running a jumper from the
green wire on the "B" switch to the red wire on the relay. Now the
"B" switch controls the diffusion pump heating element directly and
things are working fine. Hopefully this will save some time and
frustration for anyone out there who ends up with a similar problem.

Our last problem is finding the best way to thin the quartz samples.
The procedure I have followed in the preparation of the grids prior
to ion milling appears at the end of this posting.

My question is this: has anyone found a particularly advantageous
setup for the Gatan in milling a material such as a quartz arenite?
Specifically, what combinations of gun angles and voltages work best?

(For those not familiar with the Gatan 600, there are two guns firing
towards the top of the sample. The guns are linked and can be set so
both are at 20 degrees, or some combination such that gun A is at 20
degrees - x degrees and gun B is at 20 degrees + x degrees (e.g. 25 &
15 degrees), to a maximum of 40 degrees and a minimum of 0 degrees.
The current and amperage on both guns can be independently varied
from 0-4 kV (the manual recommends 0.5 mA for most materials).)



Preparation of grids:

- prepared a standard (30 um) thin section using Crystal Bond and a
Logitech thinner

- thinned by hand using diamond/resin polishing disks to approximately 20 um

- applied grids to section with epoxy, applied brass support rings to
grid with epoxy. It looks like this:

[ ] {-- brass support ring
----------- {-- grid (50 mesh Ni)
----------- {-- quartz arenite (approx. 20 um)
==================================== {-- glass slide

- material surrounding the grid was the removed with a razor blade,
the slide was heated to melt the Crystal Bond, and the
sample/grid/ring removed



Sincerely,

Kevin Macke
Bowling Green State University
Department of Geology
Bowling Green, OH
Email: macke-at-bgnet.bgsu.edu

_________________________________________________________________
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From daemon Thu Aug 2 01:37:11 2001



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Thu, 02 Aug 2001 16:24:04 +1000
Subject: SEM of coal char

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day

I have a research group interested in looking at cross sections of
coal char particles. The particles are quite porous and have been
embedded in epoxy and cross sectioned. There is very little
contrast in the SEM images as we are looking at carbon in carbon!
In order to get a reasonable image I need a fairly large spot size
(3nA, 15kV) which damages the resin which can be a large part of
the field of view. I've tried C and Au coatings, the Au reduces the
damage slightly but it is still very obvious, especially after a slow
scan.
I'm thinking a better conducting resin might help, or a more beam
resistant resin or one that gives a high contrast compared to the
char. Do such things exist? The resin cannot have particles in it
(Ag, Cu etc) as a smooth background is required for image
analysis. Any other suggestions on imaging such samples are
welcome!

Thanks

Dave




Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au



From daemon Thu Aug 2 01:46:51 2001



From: Marilena Re :      re-at-cnrsm.it
Date: Thu, 02 Aug 2001 08:41:44 +0200
Subject: tem gatan model 600 ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am Marilena Re of ENEA Brindisi (Italy). I have spoken of this problem
with my technicians, because we have two 600ion mill. The cause of the
shut off of the diffusion pump should be due to the existence of
electronic valve before, which shuts off the diffusion pump for
protection when the flow of the water is not enough to cool it. So you
should check it and control, for example, if it is dirty or if the flow
of the water is enough.
For your interest, I believe that you should ask to Gatan in order to
have the manual, because it is very useful to solve many problems.
Marilena



From daemon Thu Aug 2 05:29:07 2001



From: DANIEL EBERHARD :      daniel.eberhard-at-biologie.uni-bielefeld.de
Date: Thu, 02 Aug 2001 12:21:03 +0200
Subject: Re: Computers: PC disks in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo
have a look at the software "TransMac"
at http://www.asy.com/
This might help you.

Daniel

-----------------------------
* *
* Daniel Eberhard *
* Developmental Biology *
* & Molecular Pathology *
* University Bielefeld *
* Universitätsstr. 25 *
* 32615 Bielefeld *
* Germany *



From daemon Thu Aug 2 06:58:17 2001



From: Marilena Re :      re-at-cnrsm.it
Date: Thu, 02 Aug 2001 13:49:31 +0200
Subject: Re: TEM Gatan Model 600 Ion Mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am Marilena Re of ENEA Brindisi (Italy). I have spoken of this problem
with my technicians, because we have two 600ion mill. The cause of the
shut off of the diffusion pump should be due to the existence of
electronic valve before, which shuts off the diffusion pump for
protection when the flow of the water is not enough to cool it. So you
should check it and control, for example, if it is dirty or if the flow
of the water is enough.
For your interest, I believe that you should ask to Gatan in order to
have the manual, because it is very useful to solve many problems.
Marilena

"macke-at-bgnet.bgsu.edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have purchased Gatan Model 600 Dual Ion Mill for use in the
} preparation of foils of geological material for TEM applications.
} Since this unit was purchased used from a government lab it was
} delivered without technical support and with incomplete
} documentation, leaving many unanswered questions. We are having
} problems maintaining high vacuum (the diffusion pump shuts off) and
} cannot find the source of the problem. Help from anyone familiar
} with this device would be greatly appreciated.
}
} We are attempting to thin quartz arenites and have spent the majority
} of our time (when the unit is operational) in an effort to optimize
} the thinning process. Any input in that regard would also be welcome.
}
} Thanks in advance for any information you can provide.
}
} Kevin Macke
} Bowling Green State University
} Department of Geology
} Bowling Green, OH
} Email: macke-at-bgnet.bgsu.edu
}
} _________________________________________________________________
} Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp



From daemon Thu Aug 2 07:39:38 2001



From: Jill.Webb-at-rssl.com
Date: Thu, 2 Aug 2001 13:32:32 +0100
Subject: Vacancy for a Food Microscopist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

I am posting an advert for a vacancy we have for a food microscopist.
Please forward it to anyone you know might be interested but does not
subscribe this list.

Food Microscopist, Reading, UK

We at RSSL are looking for a Food Microscopist to join and add to our
vibrant and hard working Microscopy team. Much of the work that fully
occupies the current staff of 8½ is accounted for by our successful foreign
body analysis service, where we identify foreign materials and particulate
contamination, largely for the food and pharmaceutical industries. In
addition, we have an established special microscopy business, a large part
of which concerns food microstructure and we are looking to develop this
business and expand it to move into different food systems.

If you are a self-starter, innovative, looking for an interesting and
challenging role and believe you can make a positive contribution to our
already successful team, we would very much like to hear from you.
Ideally, you will have had a number of years practical experience of the
application of various modes of microscopy to the investigation of
different food systems and be educated to at least degree level.
The Company is situated on the University of Reading campus within pleasant
surroundings and can offer a competitive salary and benefits package,
including contributory pension, private medical cover and a Company
share-save scheme.

Please send your CV with a covering letter including your salary
expectations to Jane Cook, Human Resources Officer, Reading Scientific
Services Ltd, Lord Zuckerman Research Centre, PO Box 234, Whiteknights,
Reading, RG6 6LA, UK or e-mail Jane.cook-at-rssl.com .
RSSL is committed to providing equal employment opportunities.

Reading Scientific Services Limited is a leading provider of scientific
research, analysis and consultancy to a wide range of clients from within
the food, consumer goods, healthcare and chemical industries.
As a Company, we are committed to scientific excellence, the provision of
first class customer service and working to quality standards




Regards

Jill

Jill Webb
Principal Scientist, Microscopy, RSSL

( Office : +44 (0)118 986 8541 ext 242
( Fax : +44 (0)118 986 8932
* jill.webb-at-rssl.com






********************************************************************
This e-mail is confidential and may contain privileged
information. If you are not the addressee it may be
unlawful for you to read, copy, distribute, disclose or
otherwise use the information in this e-mail. If you are
not the intended recipient please notify us immediately.

Reading Scientific Services Ltd,
The Lord Zuckerman Research Centre,
Whiteknights, PO Box 234, Reading. RG6 6LA.

http://www.rssl.com
http://www.rssl-pharma.com
http://www.productcontamination.com
********************************************************************


From daemon Thu Aug 2 08:13:07 2001



From: JHoffpa464-at-aol.com
Date: Thu, 2 Aug 2001 09:03:59 EDT
Subject: new EM microscope purchases

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i will be starting a new core lab. we will be buying a new microscope. i need
input for a high resolution TEM with a film quality digatal camera system.
anyone have any input? was thinking either FEI or JEOL.
john hoffpauir


From daemon Thu Aug 2 09:32:47 2001



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Thu, 2 Aug 2001 08:21:01 -0600
Subject: RE: Cambridge S-250 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart,

Go for it!!

We have had a 250 Mark 3 since it was new in 1985.

It has been a rugged old SEM.

In the past few years parts are becoming increasingly difficult for even our
LEO service engineers to obtain (thank goodness it's still on service
contract).

It is a great SEM if you have large samples, need a lot of beam current and
don't do a lot of high mag work.

The 4 quadrant BSE detector is a real gem.

We use ours as a back up to our JEOL 5800.

Sometimes I love to use it, so I can twist all those knobs.!

If you need any assistance that a well used user can supply, give me a call.


William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com
} -----Original Message-----
} From: Bart Cannon [SMTP:cannonmp-at-accessone.com]
} Sent: Wednesday, August 01, 2001 3:22 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Cambridge S-250 SEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Everyone,
}
} A nicely mothballed Cambridge S-250 with many lovely accessories has
} fallen into my lap. I am contemplating bringing it back from the dead.
}
} Is there anyone among you who is still delighted their S-250 who would
} encourage me to proceed?
}
} Bart Cannon
} Cannon Microprobe
} Seattle
}


From daemon Thu Aug 2 10:14:01 2001



From: Scott J. Robinson :      sjrobin-at-itg.uiuc.edu
Date: Thu, 02 Aug 2001 10:11:10 -0500
Subject: dehydrants: mol. sieves and P2O5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi You All--

1) I just talked to a gentleman at Fullam who told me he can longer obtain
the Linde type 4A molecular sieves I've always used to keep my 100% ethanol
dry. Does anyone out there have a source for these or, perhaps more likely,
is there some great substitute for them that I'm not aware of?

2) Another quick query: can someone suggest a source for phosphorus
pentoxide in which it arrives as larger crystals (as it seems it used to)
instead of powder?

I appreciate the help I've had in the past from the listserver.

Thank you

Scott Robinson

Microscopy Suite, room B650J
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana IL 61801
217 265-5071 (office); 217 244-6219 (fax)
sjrobin-at-uiuc.edu



From daemon Thu Aug 2 11:18:04 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 2 Aug 2001 11:11:26 -0500
Subject: RE: SEM of coal char

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dave, you are using large spot size, so, I believe,
you do not need high magnifications. I do not know what
type of SEM you have, but I would suggest to try
different tilt angles (it can increase contrast, so you
can use lower current). Then you can try to use as low
voltage as possible with your scope. And if your SEM has
a possibility of frame averaging/integration, use this
feature instead of slow scan.

If nothing works, try polishing your sections with soft
clothes, it will create relief (but some distortion will
occur, and image analysis could be less precise).

Vladimir


} G'day
}
} I have a research group interested in looking at cross sections of
} coal char particles. The particles are quite porous and have been
} embedded in epoxy and cross sectioned. There is very little
} contrast in the SEM images as we are looking at carbon in carbon!
} In order to get a reasonable image I need a fairly large spot size
} (3nA, 15kV) which damages the resin which can be a large part of
} the field of view. I've tried C and Au coatings, the Au reduces the
} damage slightly but it is still very obvious, especially after a slow
} scan.
} I'm thinking a better conducting resin might help, or a more beam
} resistant resin or one that gives a high contrast compared to the
} char. Do such things exist? The resin cannot have particles in it
} (Ag, Cu etc) as a smooth background is required for image
} analysis. Any other suggestions on imaging such samples are
} welcome!
}
} Thanks
}
} Dave
}
}
}
}
} Dave Phelan
} EM/X-Ray Unit
} University of Newcastle
} NSW 2308
} AUSTRALIA
} Ph 02 4921 5667
} Fax 02 4921 7019
} emudp-at-mail.newcastle.edu.au
}
}
}


From daemon Thu Aug 2 14:09:43 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Thu, 02 Aug 2001 12:03:55 -0700
Subject: Adhering cells to cover slips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know more about methods to adhere cell culture cells to both glass and plastic cover slips. Are there any alternatives to polylysine and does it work with plastic coverslips? I currently use a .001% aqueous solution of polylysine, but by the time I take the cells through all of the dehydrations, there are not many cells left. I probably need both more cells and I thought, better adherence thru a "stickier" cover slip. I am using various cells including Hela and several oral cancer cell lines. Any other methods? Your advice is appreciated.

Barbara Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster St.
San Francisco, CA 94115
email: Bplowman-at-sf.uop.edu



From daemon Thu Aug 2 14:09:44 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Thu, 2 Aug 2001 14:58:42 -0400
Subject: plastic embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Judy,

We have several options available at Energy Beam Sciences that you may want
to consider.

The first is the Technovit 9100, Methyl Methacrylate Kit. This kit is
typically used to embed undecalcified bone. However, their is a monomer
provided with the kit, which can be mixed with the plasticiser to vary
hardness when embedding softer tissue.

Another option is the Technovit 7100, Glycol Methacrylate kit. Samples from
lung to undecalcified bone are routinely processed with Glycol Methacrylate,
therefore, hardness of the compund does not usually present an issue.

You can find these products on our web site at www.ebsciences.com. From the
home page click on General Supplies. Under the heading of Product
Information click on Light Microscopy Supplies. This will bring you to the
page with the embedding kits listed.

I hope this helps.

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Wednesday, August 01, 2001 9:11 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: Judy Trogadis


Fellow Microscopists:

I am looking for a suitable plastic embedding material which should have the
following characteristics:

- applicable for light microscopy
- not be autofluorescent
- used for immunocytochemistry, i.e. tissue should retain immunogenicity
- soft enough to cut 20 micron or thicker sections easily
- ability to embed large pieces e.g. entire rat lung
- opacity to allow identification of parts of embedded tissue (this excludes
paraffin)

I hope I am being realistic and there is a product out there to meet these
criteria. This group has never let me down.

Thank you in advance.
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca






From daemon Thu Aug 2 14:09:44 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 2 Aug 2001 12:01:46 -0700
Subject: Re: compound microscope for stained thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I need to order a compound microscope to look at thick sections from
} plastic embedded plant material before thin sectioning. Could someone
} suggest one that cost less than $2,500.00?
}
} Thanks, Nancy

You can cut a zero off that price by shopping the Chinese imports intended
primarily for the educational market. The quality is remarkably good,
considering the prices; quite good enough for your purpose. You'll find a
dealer list on the MICRO website (URL below).


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu Aug 2 17:56:16 2001



From: Palatsides, Manuela :      m.palatsides-at-pmci.unimelb.edu.au
Date: Fri, 3 Aug 2001 08:48:37 +1000
Subject: Adhering cells to cover slips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barbara
We use polylysine at 0.01% not 0.001%. See if this helps.

-----Original Message-----
} From: Barbara Plowman [mailto:Bplowman-at-sfmail.dental.uop.edu]
Sent: Friday, 3 August 2001 5:04 AM
To: Microscopy-at-sparc5.microscopy.com


I would like to know more about methods to adhere cell culture cells to
both glass and plastic cover slips. Are there any alternatives to
polylysine and does it work with plastic coverslips? I currently use a
.001% aqueous solution of polylysine, but by the time I take the cells
through all of the dehydrations, there are not many cells left. I probably
need both more cells and I thought, better adherence thru a "stickier" cover
slip. I am using various cells including Hela and several oral cancer cell
lines. Any other methods? Your advice is appreciated.

Barbara Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster St.
San Francisco, CA 94115
email: Bplowman-at-sf.uop.edu



From daemon Thu Aug 2 18:08:29 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 03 Aug 2001 09:08:05 +1000
Subject: Re: dehydrants: mol. sieves and P2O5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:11 2/08/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Use dehydrated copper sulphate. Bake crystalline copper sulphate over a
gas flame until it is a white powder. What we then do is to use dialysis
tubing as a container so we have a "sausage" of anhydrous CuSO4 which we
put in the 100% alcohol or acetaone bottle. The CuSO4 is insoluble in
alcohol and it turns blue when rehydrated - a useful indicator of the
presence of water............


}
}
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Fri Aug 3 06:24:40 2001



From: Oparowski, Joseph :      Joseph_Oparowski-at-bose.com
Date: Fri, 3 Aug 2001 07:15:24 -0400
Subject: EDX service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings listers,

Myself and a few other folks are looking for a firm to service PGT systems
in the central Massachusetts area, other than PGT. Any suggestions? Thanks
in advance

Joseph M. Oparowski
Materials Science Research - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313



From daemon Fri Aug 3 08:18:57 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thursday, August 2, 2001 10:11 AM
Subject: Fwd: dehydrants: mol. sieves and P2O5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We have used anhydrous sodium sulfate for years to keep ETOH and acetone dry. It works fine. Just add about an inch to the bottom of a pint bottle of ETOH. Discard when you use all of the ETOH.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907

--------------------------------------


Hi You All--

1) I just talked to a gentleman at Fullam who told me he can longer obtain
the Linde type 4A molecular sieves I've always used to keep my 100% ethanol
dry. Does anyone out there have a source for these or, perhaps more likely,
is there some great substitute for them that I'm not aware of?

2) Another quick query: can someone suggest a source for phosphorus
pentoxide in which it arrives as larger crystals (as it seems it used to)
instead of powder?

I appreciate the help I've had in the past from the listserver.

Thank you

Scott Robinson

Microscopy Suite, room B650J
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana IL 61801
217 265-5071 (office); 217 244-6219 (fax)
sjrobin-at-uiuc.edu





From daemon Fri Aug 3 08:41:11 2001



From: stacey andringa :      andrina-at-email.uc.edu
Date: Fri, 03 Aug 2001 09:35:50 -0400
Subject: 2 micron thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have mouse ears that were glutaraldhyde fixed, post-osmicated, ethanol
dehydrated, and embedded in Spurr. They were embedded so as to give a
cross section through the modiolus. I am trying to get serial sections
that are 2 microns thick and collected every 10 microns. The block face
is approximately 7x4 mm. There is a lot of empty plastic within the
sections that causes bubbles and folds. I have tried flattening the
sections by using 0.5% to 1% benzoyl alcohol in water in the boat, or
waving a stick dipped in acetone over the sections. I have tried
FisherBrand SuperFrost slides and FisherBrand Premium microscope
slides. I have very few sections out of hundreds that are relatively
flat. I float them on a drop of water or in a puddle of water on the
slide. I heat them on a hot plate at about 80-90° C, then transfer them
to a slide warmer at 70° C.
Any suggestions to improve flattening are desperately needed and all
will be tried!
Thanks.

Stacey Andringa
Stacey.Andringa-at-uc.email



From daemon Fri Aug 3 11:52:41 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 3 Aug 2001 12:38:59 -0400
Subject: Re: dehydrants: mol. sieves and P2O5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Use dehydrated copper sulphate. Bake crystalline copper sulphate over a
gas flame until it is a white powder. What we then do is to use dialysis
tubing as a container so we have a "sausage" of anhydrous CuSO4 which we
put in the 100% alcohol or acetaone bottle. The CuSO4 is insoluble in
alcohol and it turns blue when rehydrated - a useful indicator of the
presence of water............
Dear List,
Does anyone know the free energies and/or partition coefficients for
water in EtOH and CuSO4? I.e., if one puts 1 g of CuSO4 in 1 l EtOH what
fraction of the residual H2O is extracted from the EtOH?
TIA.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Aug 3 13:12:19 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Fri, 3 Aug 2001 14:11:01 -0400
Subject: FISH Protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

I would like some advice. I am doing FISH (Fluorescent In-Situ
Hybridization)and have been unsuccessful in getting any staining. I
am using
DAPI counterstain, but have no protocol indicating how long to stain.
The probe I am using is a dual probe (from Vysis).

If anyone is familiar with doing FISH (and successful!) and could get
in touch with me with protocols they have used,including any problem
areas, I would appreciate it!

You can contact me off the list server at sherwood-at-helix.mgh.harvard.edu.

Thanks!

Peggy Sherwood
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Fri Aug 3 15:34:42 2001



From: hagin-at-iname.com ()
Date: Sat, 4 Aug 2001 06:39:45 -0500
Subject: Ask-A-Microscopist: procedures to view dstrand or sstrand DNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Marco Arienti" {marienti-at-tiscalinet.it}
To: {"JHoffpa464-at-aol.com"-at-sparc5.microscopy.com}
Sent: Friday, August 03, 2001 7:23 AM


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (hagin-at-iname.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
August 3, 2001 at 11:17:35
---------------------------------------------------------------------------

Email: hagin-at-iname.com
Name: hagin

Education: Graduate College

Location: City, State, Country

Question: Are there ROUTINE procedures to view
dstrand or sstrand DNA in a resolution
of 20 nm ?
I have seen pictures of such macromolecules in the desired, and even
better, resolution. My question intends to find out whether this
pictures were obtained by a procedure requiring non-repeatable steps
? selection of one image out of hundreds ? trial and error ? and so
on.
Or, may be there are now procedures which can be
easily taught, and which guarantee , given some miligrams of tissue,
thousands of pictures of dna segments of hundreds of base-pairs long?
If there are, could you please refer me to the
literature which describes these procedures , or to the individuals
who have the knowledge ?
Sincerely
Yona Hagin

---------------------------------------------------------------------------


From daemon Sun Aug 5 17:45:07 2001



From: zaluzec-at-microscopy.com
Date: Sun, 5 Aug 2001 17:25:31 -0500
Subject: Administrivia: It's MM'01 Week...

Contents Retrieved from Microscopy Listserver Archives
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Colleagues

It's M&M' 01 week and that means I'm in Long Beach with
alot of your Colleagues.

Beware that means a few things will be delayed if
you need my attention as I'll be busy.

Once again we will be bringing you live streaming video
and Daily Newsletters direct from the Exhibit Floor.
Just visit the MSA HomePage.

http://www.msa.microscopy.com.


Cheers....

Nestor
Your Friendly Neighborhood SysOp.



From daemon Mon Aug 6 07:06:48 2001



From: Petra Wahlbring :      petra.wahlbring-at-crpgl.lu
Date: Mon, 06 Aug 2001 13:57:31 +0200
Subject: TEM: embedding media for PU/Polypyrrole-Foam?

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,

the specimen on my desk is a polyurethane foam (about 100 pores/inch)
covered with polypyrrole. I would like to get a ultramicrotome cut of it
for TEM anlalysis.
Can anybody suggest a preparation method? Which resin should I try for
embedding and are there any other tips and tricks about this kind of
material? I want to look at an unstained specimen and I would like to try a
staining which will allow me to distinguish between the PU and Polypyrrol
(and the resin, of course). Will RuO4 or OsO4 work with this combination?

Thanks for your input :)

Petra
--------------------------------------------------------------
Dr. Petra Wahlbring
Laboratoire d'Analyse des Materiaux (LAM)
Centre de Recherche Public - Gabriel Lippmann
162a, av. de la Faiencerie, L-1511 Luxembourg
tel. +352-470261-503
fax +352-470261-549
e-mail: petra.wahlbring-at-crpgl.lu
http://www.crpgl.lu/lam


From daemon Mon Aug 6 07:53:39 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Mon, 6 Aug 2001 08:48:30 -0400
Subject: TEM: embedding media for PU/Polypyrrole-Foam?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Years ago I did some work on foams by TEM and OM. What you do depends on whether it is a closed cell, open cell foam, how rigid the foam is and the cell size. I had to work with fairly rigid closed cell PU foams. I used an Epon clone resin for embedding with no staining. Since only the broken cells at the edges would infiltrate, I chopped up the foam into small pieces so they could be wet with Epon. Try to concentrate the randomly oriented pieces in a small volume of Epon.
In the TEM you have to search the thin sections for the structures you want to see. I was looking for particles in the cell walls and at struts.

It helps a bit to use Weck blades (Wecprep surgical blades) to make thin slices of the foam for a stereo microscopic view and a photograph of the structure you will see in the TEM. These blades are real real sharp razor blades (EM suppliers have them) and you can get some nice thin slices for doing OM using a vice and cutting the foam by hand. I had to do the cell size distributions of different PU foams using image analysis. Obviously the OM 'sections' had to be nearly as thin as the cell sizes.

In the TEM you can document the cell walls and struts if you chose your fields well. You can see the different types of struts junctions.

Hope this helps. My views are mine and not those of PPG Industries.

Paul Beauregard
Sr. Research Associate
PPG Industries
Materials Analysis Lab
Monroeville Technical Center
Monroeville, PA 15146

-----Original Message-----
} From: Petra Wahlbring [mailto:petra.wahlbring-at-crpgl.lu]
Sent: Monday, August 06, 2001 7:58 AM
To: microscopy-at-sparc5.microscopy.com


Dear listers,

the specimen on my desk is a polyurethane foam (about 100 pores/inch)
covered with polypyrrole. I would like to get a ultramicrotome cut of it
for TEM anlalysis.
Can anybody suggest a preparation method? Which resin should I try for
embedding and are there any other tips and tricks about this kind of
material? I want to look at an unstained specimen and I would like to try a
staining which will allow me to distinguish between the PU and Polypyrrol
(and the resin, of course). Will RuO4 or OsO4 work with this combination?

Thanks for your input :)

Petra
--------------------------------------------------------------
Dr. Petra Wahlbring
Laboratoire d'Analyse des Materiaux (LAM)
Centre de Recherche Public - Gabriel Lippmann
162a, av. de la Faiencerie, L-1511 Luxembourg
tel. +352-470261-503
fax +352-470261-549
e-mail: petra.wahlbring-at-crpgl.lu
http://www.crpgl.lu/lam


From daemon Mon Aug 6 11:01:31 2001



From: Robert_Hatt-at-cabot-corp.com
Date: Mon, 6 Aug 2001 11:44:55 -0400
Subject: VG STEM HB501 Available

Contents Retrieved from Microscopy Listserver Archives
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We are currently in the process of decommissioning our VG HB501-ME0766
STEM. The instrument is in working order and is currently under service
contract. Any interested parties should contact:
Larry Murphy, Manager - Cabot Analytical Laboratories.
Email: Lawrence_Murphy-at-Cabot-Corp.com. or phone (978) 670-6249.

Thank you



From daemon Mon Aug 6 11:34:49 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Mon, 6 Aug 2001 12:28:52 -0400
Subject: RE: embedding media for PU/Polypyrrole-Foam?

Contents Retrieved from Microscopy Listserver Archives
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Hi Petra,

I used to use Ted Pella's Medcast resin kit for my epoxy 'epon 812' clone material.
I was forced to switch to Eponate 12.

Some background and for your information:
Years ago, the problem with switching was that some suppliers said Epon 812 clone, identical to Epon 812 by NMR,
identical by IR, etc.

A few years ago, I ran NMRs on Eponate 12, Epon 812 (original Shell material), and Medcast. They were identical by NMR. Now I know that NMR is not a quantitative technique for assay but it was a way to screen the different clones.
I use Eponate 12 now. I have to use 70 degree for curing with Eponate 12 for some reason.

I ran one other supplier's material and it was totally different--totally different!!!

The original Shell(?) EPON 812 stuff I used as a check or reference material was and is much thicker than the material I buy and had bought recently.

Someday I will get and run other suppliers materials or kits. I may have to switch again! I suspect a lot of this material comes from the same chemical source.

Maybe someone out there has screened other material already and could send A SEPARATE EMAIL THREAD about their analysis of 'epon clones'?

Paul Beauregard
Sr. Research Associate
PPG Industries
440 College Park Drive
Monroeville, PA 15146

-----Original Message-----
} From: Petra Wahlbring [mailto:petra.wahlbring-at-crpgl.lu]
Sent: Monday, August 06, 2001 10:49 AM
To: Beauregard, Paul A.


From daemon Mon Aug 6 13:45:41 2001



From: Tom Malis :      malis-at-nrcan.gc.ca
Date: Mon, 06 Aug 2001 14:38:14 -0400
Subject: Re: TEM: embedding media for PU/Polypyrrole-Foam?

Contents Retrieved from Microscopy Listserver Archives
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Petra, we don't do much polymer work at my Lab, but I get a lot of them at
materials ultramicrotomy workshops that I do. The key point is whether or
not it is open-cell or closed-cell. If the former, it is pretty
straightforward - use a low viscosity resin like Spurrs (but some
ingredients are carcenogenic) or LR White (bit more viscous than Spurrs).
Give it lots of time to infiltrate and, if possible, do so in a low vacuum
to help encourage the air to exit the material.

If it is the latter - good luck! In 8 years of doing a hands-on workshop in
the U.S. with a crack team of 7 experienced microtomists, we have been
challenged with, and overcome, an amazing selection of students' samples for
sectioning during the course of the week. We have only failed twice; once
with a diamond-like carbon coating, but only because it was too thick (over
a micron) and we had no way of reducing the thickness. The other was an
innocent-looking, closed-cell polymer foam. Not only could we not embed it
(quite understandably), we couldn't even fracture it after immersing in
liquid nitrogen. (The outer layer would freeze but the interior remained as
'bouncy' as ever). If this is your case, I would find a FIB system and
hope that it doesn't ion damage too much during ion sectioning.

Tom Malis
Characterization Group Leader
Materials Technology Laboratory
Natural Resources Canada
Ottawa, Canada

} From: Petra Wahlbring {petra.wahlbring-at-crpgl.lu}
} Date: Mon, 06 Aug 2001 13:57:31 +0200
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM: embedding media for PU/Polypyrrole-Foam?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear listers,
}
} the specimen on my desk is a polyurethane foam (about 100 pores/inch)
} covered with polypyrrole. I would like to get a ultramicrotome cut of it
} for TEM anlalysis.
} Can anybody suggest a preparation method? Which resin should I try for
} embedding and are there any other tips and tricks about this kind of
} material? I want to look at an unstained specimen and I would like to try a
} staining which will allow me to distinguish between the PU and Polypyrrol
} (and the resin, of course). Will RuO4 or OsO4 work with this combination?
}
} Thanks for your input :)
}
} Petra
} --------------------------------------------------------------
} Dr. Petra Wahlbring
} Laboratoire d'Analyse des Materiaux (LAM)
} Centre de Recherche Public - Gabriel Lippmann
} 162a, av. de la Faiencerie, L-1511 Luxembourg
} tel. +352-470261-503
} fax +352-470261-549
} e-mail: petra.wahlbring-at-crpgl.lu
} http://www.crpgl.lu/lam



From daemon Tue Aug 7 02:52:50 2001



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Tue, 07 Aug 2001 09:34:09 +0200
Subject: JEOL 733 counting electronics - funny spikes

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Hi All,
Now this is something that I was warned out when I first pitched up
here, but has only just happened over the past couple of weeks. We're
getting random noise spikes turning up in the spectra on a couple of
spectrometers. These do not occur at the same time on both
spectrometers, nor in the same position if the spectrum is obtained
again. Sometimes these don't occur at all. It got so bad that we had to
call a halt to operations a few days ago, while we tried to suss out
what was going on. The peaks were just a mess. After sorting out a few
dodgy looking connections, the problem has all but disappeared. We still
get a few intermittant spikes but just about ok enough to get going
again. We have JEOL counting electronics JSM-XCU. These are connected in
series and we find the problem only on 2 and 4. I wonder whether there
is anybody out there who has had similar problems and found out what it
was. We've swapped things around and it's not the pre-amps, and doesn't
seem to be the modules themselves.
Any help gratefully received,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (try your luck!)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA




From daemon Tue Aug 7 08:30:41 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Tue, 7 Aug 2001 14:20:13 +0100 (BST)
Subject: Re: TEM: embedding media for PU/Polypyrrole-Foam?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Petra,

We have looked at polypyrrole by itself, so while I can't suggest anything
concerning the sectioning of the PU foam, I can think of a possibility
regarding the staining. Because both PU and PPy are nitrogenous
materials, it is likely that RuO4 and OsO4 would be overkill (though if
you are used to handling them, I wouldn't rule out trying). However,
polypyrrole is easily doped with various counterions, one of which is
iodide, which would be very electron dense, and should give good contrast.
There ought to be something in the literature about how to do the doping.

On Mon, 6 Aug 2001, Petra Wahlbring wrote:

} the specimen on my desk is a polyurethane foam (about 100 pores/inch)
} covered with polypyrrole ... I would like to try a staining which will
} allow me to distinguish between the PU and Polypyrrol (and the resin, of
} course). Will RuO4 or OsO4 work with this combination?

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+



From daemon Tue Aug 7 08:45:05 2001



From: Diego Libkind Frati :      libkind-at-crub.uncoma.edu.ar
Date: Mon, 06 Aug 2001 22:43:29 -0700
Subject: SEM of yeasts isolated from natural environments

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Hi,
I´m a biologist working with yeasts from natural environments. I would
like to know if anyone has or had experience in doing SEM and nuclear
staining with yeasts.
Or if you know someone who has.

thanks

Diego Libkind
Bariloche, Arg.



From daemon Tue Aug 7 15:39:07 2001



From: tbargar-at-unmc.edu
Date: Tue, 7 Aug 2001 15:27:52 -0500
Subject: wanted: Trimming Block for Reichert/AO Ultracut

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for a trimming block for an AO/Reichert Ultracut. The original
part description is; catalog number 9-70-17-56 Trimming Block (for serial
numbers greater than 365849).

Tom Bargar
tbargar-at-unmc.edu
1-402-559-7347



From daemon Tue Aug 7 18:48:30 2001



From: jim busse :      jsbusse-at-biochem.wisc.edu
Date: Tue, 7 Aug 2001 18:38:56 -0500
Subject: aniline blue

Contents Retrieved from Microscopy Listserver Archives
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I am interested in using aniline blue to localize callose in
botanical material. Does anyone have a reference for the absorption
and emission spectra of aniline blue under UV light?

Thanks

Jim Busse


From daemon Wed Aug 8 09:14:40 2001



From: Russell E. Cook :      recook-at-anl.gov
Date: Wed, 8 Aug 2001 08:57:51 -0600
Subject: curtains for Tecnai 20?

Contents Retrieved from Microscopy Listserver Archives
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We'll be installing a Tecnai F20 this fall. FEI recommends that the
microscope be surrounded by curtains. It is not clear to me whether the
curtains should be of a sound-attenuating material. Any advice from those
who have already installed a Tecnai 20 (of any variety) would be
appreciated.

----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov




From daemon Wed Aug 8 12:02:38 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Wed, 08 Aug 2001 12:57:44 -0400
Subject: Re: curtains for Tecnai 20?

Contents Retrieved from Microscopy Listserver Archives
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Russel,
We have installed one of the last CM 300's made and we used curtains
to create a subroom around the scope for two reasons: 1) to reduce
sound reverberations, 2) more importantly, as a focus for the air
conditioning. The air coming into the room is outside of the
curtains. The cold air falls to the floor and flows through a gap
between the floor and curtains. The heat of the scope causes a gentle
upward flow in its vicinity and is exhausted out a vent in a
"chimney" built into the ceiling. Thus, we have cooling without much
wind currents.
Ciao for now,
Ken


From daemon Wed Aug 8 12:37:53 2001



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 8 Aug 2001 12:13:35 -0500
Subject: LKB 2168 TEM grid stainer

Contents Retrieved from Microscopy Listserver Archives
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I have a used LKB 2168 TEM grid stainer needing adoption.
The unit was in working order as of its last usage (approximately 4
yrs ago).
I anticipate the system will only require a thorough cleaning prior
to entering
service at its new home. Any interested non-commercial parties
should
contact me off line for details.

Act quickly this is a limited time offer....the junk man cometh .


} John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}


From daemon Wed Aug 8 12:49:40 2001



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Wed, 08 Aug 2001 13:45:29 -0700
Subject: new pictures on-line

Contents Retrieved from Microscopy Listserver Archives
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a few snapshots of Rachel & Natasha from last month are at
http://drdcox.home.mindspring.com/family/ncc_rcc_july01.jpg



____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/



From daemon Wed Aug 8 16:01:27 2001



From: John J. Turek :      turekj-at-purdue.edu
Date: Wed, 8 Aug 2001 15:53:00 -0500
Subject: Electron Microscopist Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Life Science Microscopy Facility at Purdue University seeks an electron
microscopist for a university-wide, multi-user facility. The successful
candidate will supervise the specimen preparation section of the microscopy
facility. Duties include (1) prepare and photograph specimens for
transmission and scanning electron microscopy, (2) instruct users in the
proper use of electron and various light microscopes. (3) Participate in
laboratory courses taught by faculty (4) Develop new procedures and
techniques and furnish technical knowledge for interpretation of
micrographs. (5) Maintain billing records for service activities. The
candidate will collaborate with faculty and graduate students on research
projects and in some cases will be considered a co-author.

Minimum academic requirements are a B.S. preferably in the life sciences.
Salary is commensurate with experience. Purdue offers an outstanding
benefit package.

Purdue is an affirmative action / equal opportunity employer. Women and
minorities are encouraged to apply.

Applicants can send their CV to: (email acceptable)
John J. Turek, Ph.D.
Professor of Basic Medical Sciences
Purdue University
School of Veterinary Medicine
W. Lafayette IN, 47907-1246
Phone: 765-494-5854
Fax: 765-494-0781
email:turekj-at-purdue.edu




From daemon Wed Aug 8 16:11:11 2001



From: Richard Easingwood :      richard.easingwood-at-stonebow.otago.ac.nz
Date: Thu, 9 Aug 2001 09:18:00 +1200
Subject: TEM/STEM query

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Greetings all.

I have a small (actually not so small), but interesting problem which I
hope people out there in microscopy land may be able to help me with.

We have submitted two separate applications for replacement EM's. One
application was for a FEG-SEM with cold stage and the other for a 120kv
TEM with cryoholder. Both these configurations had been chosen after much
consultation with the researchers who use our Unit. The applications were
well supported by these researchers.

The response from our Equipment committee follows;

"Taking both applications into consideration, the committee would like to
ask you to examine the feasibility of applying for a scanning transmission
electron microscope (STEM), which the committee believe may better
facilitate research. Can you please investigate whether the purchase of
such an instrument would be a viable option for the University in enhancing
its biological science research ?"

We in the Unit believe that only a FESEM and a cryo-TEM will meet all the
needs expressed to us by researchers and that the purchase of a STEM will
mean spending a significant amount of money for a backward step in 'real
need' capability.

However I have to write a report justifying this belief.

Unfortunately I have no experience with STEM nor do I know a great deal
about STEM. As I see it a STEM with cryo-capability may well be a possible
option for our TEM researchers but my reason for not wishing to pursue the
STEM option is simply because the trade off would be that a significant
number of researchers at this University who have expressed a need for
access to a cryo-capable high resolution SEM to study frozen hydrated
'large' samples will not have their needs meet.
The purchase of a cryo-capable STEM will result in other compromises. For
example, I understand that to have a cryo-capable STEM means we would have
to compromise access to a back scattered electron detector. Due to design
considerations a cryo-capable STEM can not have a back scattered electron
detector fitted.

Any ideas to help me write my report will be gratefully received. Ideas
to the contrary of those expressed above are also very very welcome.

Replies please to Allan Mitchell, allan.mitchell-at-stonebow.otago.ac.nz.


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: office: 0064 3 479 7301
Mobile GSM: 0064 21 222 4759
Facsimile: 0064 3 479 7254
mailto:richard.easingwood-at-stonebow.otago.ac.nz
Web site: http://www.otago.ac.nz/anatomy/emunit/








From daemon Wed Aug 8 16:26:27 2001



From: Zhiping Luo :      luo-at-emcnext2.tamu.edu
Date: Wed, 08 Aug 2001 16:21:07 -0500
Subject: LOOKING FOR A USED DIMPLER

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I am looking for a used dimpler to prepare TEM samples. Any information
about it would be greatly appreciated.

Best regards,

Zhiping LUO
Research Scientist
Electron Microscopy Center
Biological Sciences Building West
Texas A&M University
College Station, TX 77843-2257
Phone: (979) 845-1129
FAX: (979) 847-8933
E-mail: luo-at-emcnext2.tamu.edu



From daemon Wed Aug 8 19:53:47 2001



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au
Date: Thu, 09 Aug 2001 10:44:30 +1000
Subject: Re: aniline blue

Contents Retrieved from Microscopy Listserver Archives
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Hi Jim,

Check out 'The study of Plant Structure: Principles and Selected
Methods' by O'Brien and McCully (1981), the bible of preparing plant
tissue for microscopy. On pg 6.98, they give the 'peak absorption
maximum for the dye-tissue complex' as ca. 370 nm, and the 'peak
fluorescence emission' as 509 nm. I hope this helps.

Mark Talbot.



From daemon Thu Aug 9 09:31:54 2001



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Thu, 9 Aug 2001 16:10:57 +0200
Subject: Y:Si EDX standard

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Dear all,
Does anybody have any good ideas about a suitable EDX standard for Y:Si?

I am working on yttrium silicates, and they tend to have fairly variable
compositions (despite the fact that they are recorded as line compositions
on the phase diagrams). Perhaps a well characterised, homogeneous glass
containing Y?

I hope there are some good ideas out there?

Thanks in advance

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/
MPI-Stuttgart (mostly in German): http://www.mpi-stuttgart.mpg.de/



From daemon Thu Aug 9 23:46:36 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 10 Aug 2001 14:37:00 +1000
Subject: RE: Si EDX standard

Contents Retrieved from Microscopy Listserver Archives
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Our Astimex WDS / EDS standards for Y use Y3Al5O12
which is a synthetic. I suppose this is used because Astimex recognized years
ago that this has better homogeneity than the Y in Si.
Disclaimer: ProSciTech supplies these standards.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, August 10, 2001 12:11 AM, Ian MacLaren
[SMTP:maclaren-at-hrem.mpi-stuttgart.mpg.de] wrote:
} { { File: ATT00008.txt; charset = Windows-1252 } }
}
}
} Dear all,
} Does anybody have any good ideas about a suitable EDX standard for Y:Si?
}
} I am working on yttrium silicates, and they tend to have fairly variable
} compositions (despite the fact that they are recorded as line compositions
} on the phase diagrams). Perhaps a well characterised, homogeneous glass
} containing Y?
}
} I hope there are some good ideas out there?
}
} Thanks in advance
}
} Ian MacLaren
} Max Planck Institut fur Metallforschung, Seestra?e 92,
} 70174 Stuttgart, Germany
} Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
} Home page: http://members.tripod.co.uk/IanMacLaren/
} MPI-Stuttgart (mostly in German): http://www.mpi-stuttgart.mpg.de/


From daemon Fri Aug 10 13:11:48 2001



From: Kirk Czymmek :      kirk-at-udel.edu
Date: Fri, 10 Aug 2001 14:11:12 -0400
Subject: Research Associate Position Available

Contents Retrieved from Microscopy Listserver Archives
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Research Associate I – Bioimaging/Microscopy

The Delaware Biotechnology Institute (DBI) at the University of Delaware has
an immediate opening for a Research Associate I in its state-of-the-art
BioImaging Center. The successful candidate should have a BS/MS in Biology
or related field and a strong background (2+ years) in microscopy. The
BioImaging Center includes an array of microscopy equipment including
conventional fluorescence, confocal, multi-photon, atomic force,
transmission and field emission scanning electron microscopes and their
ancillary sample preparation equipment.

The primary responsibilities of the position include assisting the director
of the BioImaging Center with general day-to-day operation of the multi-user
facility as well as user training, equipment scheduling, and report
generation. Experience in a multi-user facility and good familiarity with
digital image processing and output, confocal microscopy and biological EM
sample preparation are desirable. The individual must have strong
communication and organizational skills, be motivated to learn new
techniques, flexible, and have the ability to interact with a diverse group
of research personnel. The highly variable nature of projects and
collaborations as well as the excellent facilities, provides tremendous
opportunity for professional growth.

The salary for the position will be commensurate with successful candidate
qualifications. Applicants should send a cover letter, resume/CV, and list
of 3 references to Professor Kirk J. Czymmek, 15 Innovation Way, Delaware
Biotechnology Institute, University of Delaware, Newark, DE 19711. Email:
kirk-at-udel.edu

Applications will be accepted until August 20, 2000 or until the position is
filled.

The University of Delaware is an Equal Opportunity Employer, which
encourages applications from Minority Group Members and Women.



From daemon Fri Aug 10 17:11:37 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 10 Aug 2001 15:03:18 -0700
Subject: Re: LOOKING FOR A USED DIMPLER

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Luo:

We do have a used D500 Dimpler that just became available. This is not
the
same as our newer version D500i, but it is still an excellent product.
If
you have an interest, please contact me off-line.

Best regards-

David
--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***

Zhiping Luo wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All,
}
} I am looking for a used dimpler to prepare TEM samples. Any information
} about it would be greatly appreciated.
}
} Best regards,
}
} Zhiping LUO
} Research Scientist
} Electron Microscopy Center
} Biological Sciences Building West
} Texas A&M University
} College Station, TX 77843-2257
} Phone: (979) 845-1129
} FAX: (979) 847-8933
} E-mail: luo-at-emcnext2.tamu.edu


From daemon Sat Aug 11 03:16:41 2001



From: uadmon-at-netvision.net.il
Date: Sat, 11 Aug 2001 11:01:23 +0300
Subject: connect Coolpix 990 to light microscope

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Connect Coolpix 990 to a light microscope

Can anybody help?

The problem: to install a Nikon Coolpix 990 on
a MEF-3 Reichert-Jung (metallurgical) light
microscope.

We bought from Nikon the MCD lens adapter,
which fits well the standard port for a CCD
camera on the left-hand side of the microscope
– actually a “T” connection on the “Rotoscope”
screen support tube.

However:

(1) The field-of-view grabbed and displayed by the
Coolpix is considerably smaller (just one quarter
linear size) than the field-of-view of the Polaroid
camera or that seen through the binoculars, for
the same specimen and microscope setting. It is
noteworthy that the image itself is distortion-free
and of high quality.

(2) No in-focus image can be obtained when the
microscope is set to magnification of x50 and
below.

(3) The same problems appeared also when fitting
a CCD camera through the same port.

(4) The problem can be solved by mounting the
Coolpix instead of one of the two eyepices (occulars).
Obviously, this is a bad solution, as the microscope
is left with only one eyepiece.

Is there a remedy?


Thanx,

Dr. Uri Admon

Beer-Sheva, Israel





From daemon Sat Aug 11 09:41:40 2001



From: MICROFAB-at-aol.com
Date: Sat, 11 Aug 2001 09:34:39 -0500
Subject: Re: connect Coolpix 990 to light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen an adaptor invented and marketed by Martin Microscope Company
designed for the Cool Pix camera. It was quite impressive. I have no
financial ties to the company above.

You can get more info at the following URL:

http://www.martinmicroscope.com/martinmicro001.htm

Jim Harper
microfab-at-aol.com





In a message dated 8/11/2001 7:47:49 AM Eastern Daylight Time,
uadmon-at-netvision.net.il-at-sparc5.microscopy.com writes:

} Hi all,
}
} Connect Coolpix 990 to a light microscope
}
} Can anybody help?
}


From daemon Sat Aug 11 09:55:26 2001



From: ARSEG-at-aol.com ()
Date: Sat, 11 Aug 2001 09:51:30 -0500
Subject: Ask-A-Microscopist: slide to observe mycorrhizal relationships.

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ARSEG -at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
August 10, 2001 at 18:21:39
---------------------------------------------------------------------------

Email: ARSEG -at-aol.com
Name: Rene Griffith

Organization: Union

Education: 9-12th Grade High School

Location: City, State, Country

Question: Hi my name is rene.I am a researcher at union highscool and
i will be developing a slide to observe mycorrhizal relationships. So
i would like to know what mountant you think i can use?

---------------------------------------------------------------------------


From daemon Sat Aug 11 11:16:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 11 Aug 2001 09:10:29 -0700
Subject: Re: connect Coolpix 990 to light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'm not sure about your specific microscope model, but
I suspect that Optem has an adapter which will fit it.

Optem makes a very nice adapter system for the 990.
The top piece is PN 257015 which mates to PN 29-90-56.
This then mates to a standard 1X C-mount on the
trinoc port of the microscope. The lens inside the
adapters provides the correct field of view to match
that seen through the oculars.

Optem is in Fairport, NY USA and can be reached
at info-at-optemintl.com or URL http://www.optemintl.com

gary g.


At 01:01 AM 8/11/2001, you wrote:

} Hi all,
}
} Connect Coolpix 990 to a light microscope
}
} Can anybody help?
}
} The problem: to install a Nikon Coolpix 990 on
} a MEF-3 Reichert-Jung (metallurgical) light
} microscope.
}
} We bought from Nikon the MCD lens adapter,
} which fits well the standard port for a CCD
} camera on the left-hand side of the microscope
} ­ actually a "T" connection on the "Rotoscope"
} screen support tube.
}
} However:
}
} (1) The field-of-view grabbed and displayed by the
} Coolpix is considerably smaller (just one quarter
} linear size) than the field-of-view of the Polaroid
} camera or that seen through the binoculars, for
} the same specimen and microscope setting. It is
} noteworthy that the image itself is distortion-free
} and of high quality.
}
} (2) No in-focus image can be obtained when the
} microscope is set to magnification of x50 and
} below.
}
} (3) The same problems appeared also when fitting
} a CCD camera through the same port.
}
} (4) The problem can be solved by mounting the
} Coolpix instead of one of the two eyepices (occulars).
} Obviously, this is a bad solution, as the microscope
} is left with only one eyepiece.
}
} Is there a remedy?
}
}
} Thanx,
}
} Dr. Uri Admon
}
} Beer-Sheva, Israel



From daemon Sun Aug 12 00:33:29 2001



From: Edward_Principe-at-amat.com
Date: Sun, 12 Aug 2001 00:21:34 -0500
Subject: 3D SEM Reconstruction

Contents Retrieved from Microscopy Listserver Archives
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I am wondering if anyone can help point me in the right direction regarding
status of research, or better yet, ready to go software for computing 3D
reconstruction of an SEM image.

My particular interest is defects on wafer surfaces. I have seen stereo
pairs and some materials on reconstruction from stereo pairs, but not too
impressed so far for my needs (not to say I have not seen some dandy stero
pair SEM images).

I was thinking one could acquire multiple views (i.e., analogous to top,
front, right side, rear, left side ) accomplished by rotation and tilt in
the SEM. Then perhaps with a little help from the user to define common
points in the various views, one to reconstruct the image. I am
oversimplifying and no offense to the image experts, but the rest is math
and graphics.

Anyone know of something like this or functionally equivalent ?

Regards,
Ed Principe
Defect & Thin Film Characterization Laboratory
Applied Materials


From daemon Sun Aug 12 12:34:37 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 12 Aug 2001 13:25:09 -0500
Subject: 3D reconstruction software

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ed Principe wrote:
==============================================================
I am wondering if anyone can help point me in the right direction regarding
status of research, or better yet, ready to go software for computing 3D
reconstruction of an SEM image.

My particular interest is defects on wafer surfaces. I have seen stereo
pairs and some materials on reconstruction from stereo pairs, but not too
impressed so far for my needs (not to say I have not seen some dandy stero
pair SEM images).

I was thinking one could acquire multiple views (i.e., analogous to top,
front, right side, rear, left side ) accomplished by rotation and tilt in
the SEM. Then perhaps with a little help from the user to define common
points in the various views, one to reconstruct the image. I am
oversimplifying and no offense to the image experts, but the rest is math
and graphics.

Anyone know of something like this or functionally equivalent ?
===============================================================
Two such firms offering this kind of product are

1] Alicona (Germany) and their MeX 3D Reconstruction Software from SEM
images
www.alicona.com

and

2] Soft Imaging Systems (also Germany)
http://www.soft-imaging.de/

There are some differences between the two products, including price. We
have use both in our own laboratory and find them both quite acceptable.

Disclaimer: SPI Supplies will be distributing the Alicona product in the
near future.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sun Aug 12 16:05:52 2001



From: DrJohnRuss-at-aol.com
Date: Sun, 12 Aug 2001 16:58:59 EDT
Subject: Re: 3D reconstruction software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 8/12/01 1:43:03 PM, cgarber-at-2spi.com writes:

} My particular interest is defects on wafer surfaces. I have seen stereo
} pairs and some materials on reconstruction from stereo pairs, but not too
} impressed so far for my needs (not to say I have not seen some dandy stero
} pair SEM images).
}
} I was thinking one could acquire multiple views (i.e., analogous to top,
} front, right side, rear, left side ) accomplished by rotation and tilt
} in
} the SEM. Then perhaps with a little help from the user to define common
} points in the various views, one to reconstruct the image. I am
} oversimplifying and no offense to the image experts, but the rest is math
} and graphics.
}
} Anyone know of something like this or functionally equivalent ?

Fovea Pro (http://reindeergraphics.com) includes this capability, as do
several more costly packages. The Fovea routine functions as a plug-in within
Photoshop. It uses cross-correlation to match points between the images,
producing an image in which the horizontal displacement (parallax) is
recorded as a grey scale value. This image can be measured (e.g., line
profiles, etc.) or used to render perspective-corrected graphic images of the
surface.


From daemon Sun Aug 12 20:15:44 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 12 Aug 2001 18:09:21 -0700
Subject: 3D SEM Reconstruction

Contents Retrieved from Microscopy Listserver Archives
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} Date: Sun, 12 Aug 2001 09:04:08 -0700
} To: Edward_Principe-at-amat.com
} From: Gary Gaugler {gary-at-microtechnics.com}
} Subject: Re: 3D SEM Reconstruction
}
}
} Take a look at AutoQuant 3D reconstruction software.
} I use it an I am an authorized dealer for AQI products.
}
} You can see more about their Autovisualize 3D at
}
} http://www.aqi.com
}
} gary gaugler
} 916.791.8191 (Calif.)
}
}
} At 10:21 PM 8/11/2001, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Aug 13 08:46:47 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Mon, 13 Aug 2001 08:41:36 -0500
Subject: coolpix 990

Contents Retrieved from Microscopy Listserver Archives
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Greetings:

We have a Reichert MeF-3 microscope and have mounted a Kodak
Megaplus 1.4 on the right side, which is a port for ther macro camera. A
coupling lens from Diagnostic Instruments was needed to fill the camera
detector. We had mounted it on the left side but in re-arranging the room we
moved it to the other side without difficulty.

Best Regards

Sam Purdy



Technical Center
National Steel Corp.
1745 Fritz Dr.
Trenton MI
Voice:734-676-2682
FAX: 734-676-2682
spurdy-at-nationalsteel.com


From daemon Mon Aug 13 08:46:47 2001



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Mon, 13 Aug 2001 08:30:35 -0500
Subject: TEM/SEM Position Available

Contents Retrieved from Microscopy Listserver Archives
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--
Electron Microscopy position available. Multi-user;
clinical/research facility. Call Valerie Sinor -at- 210-567-2607 for
more information.
UTHSCSA, Department of Pathology, San Antonio, Texas
EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EMPLOYER


From daemon Mon Aug 13 15:50:23 2001



From: Margaret Miller :      MILLERMM-at-uthscsa.edu
Date: Mon, 13 Aug 2001 15:37:45 -0500
Subject: TEM/SEM Position Available

Contents Retrieved from Microscopy Listserver Archives
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--
Electron Microscopy position available. Multi-user;
clinical/research facility. Call Valerie Sinor -at- 210-567-2607 for
more information.
UTHSCSA, Department of Pathology, San Antonio, Texas
EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EMPLOYER


From daemon Mon Aug 13 23:06:07 2001



From: Terry Robertson :      terryr-at-cyllene.uwa.edu.au
Date: Tue, 14 Aug 2001 11:57:47 +0800
Subject: Methenamine silver staining of basement membranes

Contents Retrieved from Microscopy Listserver Archives
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Does anyone out there in cyber space have any experience in dealing with periodic acid methenamine silver staining of renal basement membranes at the ultrastructural level (that is thin sections). Any help would be gratefully appreciated

Many thanks

Terry


Dr Terry Robertson (PhD)
Senior Research Fellow
Department of Pathology
University of Western Australia
Nedlands 6009

Phone 618 9346 2935
Fax 618 9346 2891
Mobile phone 040302 5440
email terryr-at-cyllene.uwa.edu.au




From daemon Tue Aug 14 11:20:20 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 14 Aug 2001 09:03:00 -0700
Subject: Re: TEM/STEM query

Contents Retrieved from Microscopy Listserver Archives
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Dear Richard,
While an STEM may be a viable substitute for a TEM for some applications, it
cannot do what an SEM can do.
1. STEM runs at 100 to 200 kV accelerating voltage for beam penetration, the
opposite of the surface studies you want to do at 1 to 20 kV on a FESEM.
2. Sample size will be restricted to a standard TEM holder: 3 mm in diameter
and 0.5 mm thick. Most SEM samples are 10 mm cubed or larger.
3. A modern STEM will cost about twice as much as a FESEM.
The only dedicated STEM I saw in the Exhibits of the M&M 2001 last week was
aimed at the semi-conductor testing market and there were no biological
application mentioned. I have a TEM with scanning option, but the
restrictions above also apply to it. The STEM mode is used for EDX testing.
Anyone who thinks an STEM can substitute for an SEM has no knowledge of
either technology.
At 09:18 AM 8/9/01 +1200, you wrote:
}
} Greetings all.
}
} I have a small (actually not so small), but interesting problem which I
} hope people out there in microscopy land may be able to help me with.
}
} We have submitted two separate applications for replacement EM's. One
} application was for a FEG-SEM with cold stage and the other for a 120kv
} TEM with cryoholder. Both these configurations had been chosen after much
} consultation with the researchers who use our Unit. The applications were
} well supported by these researchers.
}
} The response from our Equipment committee follows;
}
} "Taking both applications into consideration, the committee would like to
} ask you to examine the feasibility of applying for a scanning transmission
} electron microscope (STEM), which the committee believe may better
} facilitate research. Can you please investigate whether the purchase of
} such an instrument would be a viable option for the University in enhancing
} its biological science research ?"
}
} We in the Unit believe that only a FESEM and a cryo-TEM will meet all the
} needs expressed to us by researchers and that the purchase of a STEM will
} mean spending a significant amount of money for a backward step in 'real
} need' capability.
}
} However I have to write a report justifying this belief.
}
} Unfortunately I have no experience with STEM nor do I know a great deal
} about STEM. As I see it a STEM with cryo-capability may well be a possible
} option for our TEM researchers but my reason for not wishing to pursue the
} STEM option is simply because the trade off would be that a significant
} number of researchers at this University who have expressed a need for
} access to a cryo-capable high resolution SEM to study frozen hydrated
} 'large' samples will not have their needs meet.
} The purchase of a cryo-capable STEM will result in other compromises. For
} example, I understand that to have a cryo-capable STEM means we would have
} to compromise access to a back scattered electron detector. Due to design
} considerations a cryo-capable STEM can not have a back scattered electron
} detector fitted.
}
} Any ideas to help me write my report will be gratefully received. Ideas
} to the contrary of those expressed above are also very very welcome.
}
} Replies please to Allan Mitchell, allan.mitchell-at-stonebow.otago.ac.nz.
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} School of Medical Sciences, University of Otago
} PO Box 913, Dunedin
} NEW ZEALAND

Regards and good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Aug 14 11:37:27 2001



From: Pankaj Kumar Patro :      pankaj-at-met.iitb.ac.in
Date: Tue, 14 Aug 2001 22:00:23 +0000 (IST)
Subject: SEM,CAMECA SU-30, CRT

Contents Retrieved from Microscopy Listserver Archives
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Dear listners

Our group uses a CAMECA SU 30 SEM Probe.Recently it has developed a
problem in CRT.The image starts shaking when it is continued to be on for
some time.We thought it may starts heating up when is kept on so we put
a cooling fan in it and also replaced the old heat sinks in the circuits
with new ones. Still we could not solve the problem although it decreased
to a little extent.

I do hope to get suggestions to overcome the problems.

Thanks in advance

Regards

Mr. Pankaj Kumar Patro
PhD student
IIT-Bombay
Mumbai,India

e-mail-:pankaj-at-met.iitb.ac.in



From daemon Tue Aug 14 12:12:51 2001



From: Mike Delannoy :      delannoy-at-mail.jhmi.edu
Date: Tue, 14 Aug 2001 13:02:31 -0400
Subject: membrane sloughing/blebbing

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Anyone know what non and artifactual causes are there for membrane
blebbing or sloughing of parasite intected intestinal cells. We know
about glut and over microwaving
(insufficient lipid fix and overheating) anything else?
thanks
Mike D



From daemon Tue Aug 14 14:59:14 2001



From: Weiliang Gong :      gongw-at-vsl.cua.edu
Date: Tue, 14 Aug 2001 14:33:40 -0500 (EST)
Subject: Any comments?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We are going to purchase an electropolisher for our electron microscopy
lab. We intend to use the polisher to prepare metallic TEM samples.
Right now I have three candidates, Fishione Model 110 Twin-Jet
Electropolisher, Struers TenuPol-5, and South Bay Model 550D. Can
anyone with experiences using one of the polishers make comments to
help us make decision?

Please contact me offline. My meail address: gongw-at-vsl.cua.edu

Thanks in advance,

Wei Gong



From daemon Tue Aug 14 14:59:16 2001



From: robert.fowler-at-tdktca.com
Date: Tue, 14 Aug 2001 15:54:22 -0400
Subject: PROBLEM WITH JEOL T220A

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Dear Listers,
I am having some trouble out of my SEM. Problem is in evacuation no led's
are lighting up on the sequence panel. I can hear the rotary pump running,
but I am not sure if it is actually working. So far I have checked the
rotary pump oil level. Disassembled cleaned and reassembled the RP Vent
valve. Checked the diffusion pump heater in and out of circuit. And of
course the fuses. Working voltage is 100 volt AC and line voltage as
checked is running at 108 volts. Switching to VAC on Checker Meter shows
zero..........Any help would be appreciated
Thank you in advance

p.s. VITALY FEINGOLD are you still out there I have lost your e-mail
address

Robert Fowler
Quality Assurance Technician
TDK Components USA, Inc.
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


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TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
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(847-803-6100). Thank you.




From daemon Tue Aug 14 14:59:16 2001



From: Weiliang Gong :      gongw-at-vsl.cua.edu
Date: Tue, 14 Aug 2001 14:36:21 -0500 (EST)
Subject: Need your comments?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We are going to purchase an electropolisher for our electron microscopy
lab. We intend to use the polisher to prepare metallic TEM samples.
Right now I have three candidates, Fishione Model 110 Twin-Jet
Electropolisher, Struers TenuPol-5, and South Bay Model 550D. Can
anyone with experiences using one of the polishers make comments to
help us make decision?

Please contact me offline. My meail address: gongw-at-vsl.cua.edu

Thanks in advance,

Wei Gong



From daemon Tue Aug 14 22:28:35 2001



From: Edward_Principe-at-amat.com
Date: Tue, 14 Aug 2001 20:19:47 -0700
Subject: Re: TEM/STEM query

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I would say the very crudest breakdown of STEM and TEM is:
TEM can produce some of the highest signal/noise high resolution phase
images (i.e., TEM is good for imaging). The extreme example is 'true'
HRTEM.
STEM is primarily valued for its spectroscopic analytical capability (not
implying at all TEM does not provide analytical capability). The very
latest/greatest HRTEM can match STEM image resolution. The STEM's most
common imaging mode, high angle dark field, is primarily a Z-contrast
image. Today's latest TEM/STEM systems can operate in STEM mode with a
~1.4A (300kV) or ~1.9A (200kV) and spot size with sufficient current to
collect (P)EELS and EDS data in 5-20s per point (S/N issue). With a
monochromoter, source correction, Cs hardware correction and the latest
energy filter/energy loss spectrometer; you can approach meV energy
resolution (should be coming "soon"). This is a change from earlier
generations of TEM/STEM, where only a dedicated STEM in a research level
facility could approach this spot resolution. The jist is; today or soon,
you can really get the best of both worlds in a single commercial
instrument. Obviously, I am a bit excited about the possibilities.

I think it really comes down to this: Do you need technology at your
disposal that will allow you to examine the composition and chemistry of
interfaces, cross-sections and surfaces at near atomic resolution by the
methods accessible by TEM/STEM.....and you got money (~$2.5M for the
hardware)? If yes, you need a STEM. Can you live with pictures and
generic elemental EDS/WDS to get the information you want ? If yes,
SEM-based information for you. SEM-Based STEM is somewhere in between, but
much closer to SEM than STEM. You can approach some of the same image
gains provided by STEM and also some of the enhanced spatial resolution EDS
benefits by working with a TEM thin ( {2kA) sample on a SEM-based STEM
system, but really not the same thing. But, you might have a practical
niche.

I omitted a lot of caveats to the above statements, and there are usually
multiple ways (analytical approaches) to achieve any given end, but that
is the big picture. STEM is up there in terms of near atomic resolution
chemical / elemental information.

You mentioned biological research. That niche in particular has a host of
"pecadillos" and you might need to track down an expert or five when
thinking about STEM. I have zero biological experience, so can offer no
bio-specific help. But, you got a lot of current in a small spot. PEELS
and especially GIF offer some time/spot advantages that I would find
generically appealing if I were in a biological field, but I'm not. I bet
there are people who know. How many people are using STEM/cryo in
biological research? Why? I also can't comment of geometry and
manufacturing limits for cryo STEM verses back-scatter detector.

Regarding SEM in general; well, that is a different type of information
access. SEM yields images that when supplemented with various
analyzers/detectors can provide topography info (standard detectors,
immersion lens, multi-perspective detectors, stereo pair images, etc),
"material contrast" info (i.e., BSE detector) and chemical information
(primarily EDS and less common WDS/micro-probe), as well as some structural
information (including BSED..back scatter electron diffraction). Peolple
even stick more exotic and generally less robust analyzers (ie., electron
flight tube/AES) on a SEM-based tool. I know I am forgetting a host of
other relevant information. There are people who specialize in SEM for a
career who know much much better.

SEM sample prep is less demanding, in general, but is also an art form and
widely varied (biological could be the tougher). TEM sample prep is
demanding, again being very general, and until very recently, was also
very dependent on an individual's skill. With the advent of FIB-based
"semi-automatic" TEM sample prep, things are getting interesting, if not
easier (may not apply to bio applications, ....or could it?). But, there
is still no replacement for a skilled operator and a sample prep expert.
You can only analyze what you can prepare successfully.

Facilities costs will be considerably higher for a good TEM/STEM operation.
The amount of work anticipated is always a factor when justifying capital.
Just biological ? Anybody else to support the capital?

Regarding TEM/STEM, there is also a difference in an operator skilled at
"imaging" (TEM) and an operator skilled at "analysis" (let's say STEM).
The latter is more rare, but there is high value if you find one who fits
your bill. I believe TEM/STEM is on the verge of some exciting new growth
in terms of applications and general utility.

BIG Disclaimer: I do not represent or work for any SEM, TEM, or STEM
manufacturer.

Much more than you likely wanted, but I hope it helps. You may have
stumbled into a can of worms. But, I can sympathize with those "beneath"
you who count on your educated decision and well-written report. Please
appreciate the complexity of the decision matrix laid before you. It may
pay significantly if you make the right decision and conversely, costly in
terms of either opportunity or actual hardware cost if you make a poor
decision.

Good Luck. I'd be intrigued to know the final decision and reasoning.
Regards,
Ed

*************************************************************

Edward L. Principe, Ph.D.
Member of Technical Staff
Defect & Thin Film Characterization Laboratory
Applied Materials





Mary Mager {mager-at-interchange.ubc.ca} on 08/14/2001 09:03:00 AM




To: Richard Easingwood {richard.easingwood-at-stonebow.otago.ac.nz}
cc: Microscopy-at-sparc5.microscopy.com


Dear Richard,
While an STEM may be a viable substitute for a TEM for some applications,
it
cannot do what an SEM can do.
1. STEM runs at 100 to 200 kV accelerating voltage for beam penetration,
the
opposite of the surface studies you want to do at 1 to 20 kV on a FESEM.
2. Sample size will be restricted to a standard TEM holder: 3 mm in
diameter
and 0.5 mm thick. Most SEM samples are 10 mm cubed or larger.
3. A modern STEM will cost about twice as much as a FESEM.
The only dedicated STEM I saw in the Exhibits of the M&M 2001 last week was
aimed at the semi-conductor testing market and there were no biological
application mentioned. I have a TEM with scanning option, but the
restrictions above also apply to it. The STEM mode is used for EDX testing.
Anyone who thinks an STEM can substitute for an SEM has no knowledge of
either technology.
At 09:18 AM 8/9/01 +1200, you wrote:
}
} Greetings all.
}
} I have a small (actually not so small), but interesting problem which I
} hope people out there in microscopy land may be able to help me with.
}
} We have submitted two separate applications for replacement EM's. One
} application was for a FEG-SEM with cold stage and the other for a 120kv
} TEM with cryoholder. Both these configurations had been chosen after much
} consultation with the researchers who use our Unit. The applications were
} well supported by these researchers.
}
} The response from our Equipment committee follows;
}
} "Taking both applications into consideration, the committee would like to
} ask you to examine the feasibility of applying for a scanning transmission
} electron microscope (STEM), which the committee believe may better
} facilitate research. Can you please investigate whether the purchase of
} such an instrument would be a viable option for the University in
enhancing
} its biological science research ?"
}
} We in the Unit believe that only a FESEM and a cryo-TEM will meet all the
} needs expressed to us by researchers and that the purchase of a STEM will
} mean spending a significant amount of money for a backward step in 'real
} need' capability.
}
} However I have to write a report justifying this belief.
}
} Unfortunately I have no experience with STEM nor do I know a great deal
} about STEM. As I see it a STEM with cryo-capability may well be a possible
} option for our TEM researchers but my reason for not wishing to pursue the
} STEM option is simply because the trade off would be that a significant
} number of researchers at this University who have expressed a need for
} access to a cryo-capable high resolution SEM to study frozen hydrated
} 'large' samples will not have their needs meet.
} The purchase of a cryo-capable STEM will result in other compromises. For
} example, I understand that to have a cryo-capable STEM means we would
have
} to compromise access to a back scattered electron detector. Due to design
} considerations a cryo-capable STEM can not have a back scattered electron
} detector fitted.
}
} Any ideas to help me write my report will be gratefully received. Ideas
} to the contrary of those expressed above are also very very welcome.
}
} Replies please to Allan Mitchell, allan.mitchell-at-stonebow.otago.ac.nz.
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} School of Medical Sciences, University of Otago
} PO Box 913, Dunedin
} NEW ZEALAND

Regards and good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca






From daemon Tue Aug 14 22:29:49 2001



From: David Burton :      dburton-at-nwlink.com
Date: Tue, 14 Aug 2001 20:29:41 -0700
Subject: Plastic coverslips

Contents Retrieved from Microscopy Listserver Archives
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Any experience with plastic coverslipping machines? We are having problems
with poor resolution from our two year old system. Looking at a glass cover
slipped slide the microscope resolution is great, changing to one of the
plastic ones and it's not so good... It could be processing or the plastic,
the glass cover slipped slides are done by a different facility.

Any suggestions appreciated!



From daemon Wed Aug 15 07:09:45 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 15 Aug 2001 07:56:07 -0400
Subject: Semiconductor Contaminants

Contents Retrieved from Microscopy Listserver Archives
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Does anyone out there know of a good source, text or website, that deals
with the issue of contaminants in a semiconductor manufacturing environment?
I'm putting together a lecture on the subject but I don't want to generate
SEM's, optical images etc. if this is available readily. Principally I'm
interested in human spittle, airborne contaminates such as skin, hair, cloth
fibers, etc. or anything that may be generated by human interaction with a
semiconductor device.

Regards,
Peter Tomic
Anadigics, Inc.


From daemon Wed Aug 15 07:09:49 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 15 Aug 2001 04:58:27 -0700 (PDT)
Subject: Re: membrane sloughing/blebbing

Contents Retrieved from Microscopy Listserver Archives
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Mike:
Back when biological SEM was new, there was a lot of excitement over the use
of cell surface blebbing, etc to differentiate between T and B cells. Well,
the upshot of things (and I forget the references) was that the heating of
the sample during critical point drying was causing the blebbing, ruffles,
etc. And this was after both glutaraldehyde and osmium fixation. So, my
guess is that that the blebbing is caused by heating. Membranes are still
very mobile (the lipid phases, that is) following fixation, and driving the
temp up to 50C or higher will probably give you some artifacts. Question
is, can you live with those artifacts? I would also recommend that you
check the Ted Pella, Inc website (http://www.tedpella.com) for protocols
using the microwave at lower temps. There were a number of excellent papers
at last week's Microscopy & Microanalysis meeting using these techniques.
Steve Slap at Hacker might also be a good resource for you here.
Roger
On Tue, 14 Aug 2001 13:02:31 -0400, Mike Delannoy wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hello,
| Anyone know what non and artifactual causes are there for membrane
| blebbing or sloughing of parasite intected intestinal cells. We know
| about glut and over microwaving
| (insufficient lipid fix and overheating) anything else?
| thanks
| Mike D
|
|


Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Wed Aug 15 09:03:14 2001



From: Tony Owens :      towens-at-camscan-usa.com
Date: Wed, 15 Aug 2001 09:52:58 -0400
Subject: SirCam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

I've received at least a dozen emails sent by this virus over the last
few weeks, so watch out for it. I highly recommend viewing messages on
the mail server BEFORE downloading your mail (use message dispatcher
in The Bat mail client). Then you can delete the message directly from
the server.

} You may receive a message sent by SirCam virus. The body of the
} message is randomly chosen, although the first and last lines are
} always "Hi! How are you?" and "See you later. Thanks" in the English
} version of the message and "Hola como estas?" and "Nos vemos pronto,
} gracias" in the Spanish version. The message always have a file
} attachment with double extension, which is also randomly choosen,
} e.g .doc.bat, .doc.lnk, .xls.bat. Altough The Bat! will warn you
} before opening such files or blocks them completely, do not try to
} open them!



Tony mailto:towens-at-camscan-usa.com



From daemon Wed Aug 15 09:20:06 2001



From: zaluzec-at-microscopy.com
Date: Wed, 15 Aug 2001 09:15:13 -0500
Subject: Administrivia:SirCam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

You will not get a copy of the SirCam virus by way of the
Microscopy Listserver. My software has been blocking it
fine (for now), however, the server is slow now because of the
number of attacks I'm getting. Each attempt as Tony
points out has an attached file which is starting to cause
headaches for me as the server drives are filling and
emptying constantly. When the drives fill we hit a
bottle neck and the server grinds to a halt until the
junk mail and it's attachments are cleared.

Nestor
Your Friendly Neighborhood SysOp.

PS, I wish I was getting only a dozen attempt over
a few week period. I've been getting over 500/day
as of this weekend. Sigh..the joys of being a SysOp...



From daemon Wed Aug 15 10:43:40 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Wed, 15 Aug 2001 11:36:44 -0400 (EDT)
Subject: Refractometer?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers...

I am looking for someone in the NYC/NJ area who has a refractometer to do one measurement of the refractive
index of a glycerin/gelatin solution.

Thanks and best,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977




From daemon Wed Aug 15 21:53:10 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 15 Aug 2001 16:43:10 -1000 (HST)
Subject: LM of resin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, All-

It sure was nice to see so many of you at M&M 2001 in Long Beach last
week!

I generally only look at 0.5 to 2.0 micrometer epoxy or acrylic sections
in the light microscope to see where in the block I am before taking
ultrathin sections for TEM. I stain the thick sections with toluidine
blue, glance at them and then throw them away. However, now I want to keep
some of them and get good photomicrographs as well. In the past when I
coverslipped them and took (35 mm film) pictures, they looked pretty
bad. I've heard of various fixes, such as using thicker coverslips.

Now I'd like to hear from someone doing this routinely, with good
results (if possible!). What kind of mounting media? What kind of
coverslips? Why? What goes on with the light as it passes through the
resin?

What kind of optics? Phase? Hoffman? DIC? Plain vanilla BF?

Any clues will be appreciated!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Aug 16 08:01:19 2001



From: robert.fowler-at-tdktca.com
Date: Thu, 16 Aug 2001 08:48:40 -0400
Subject: Need Stain ??

Contents Retrieved from Microscopy Listserver Archives
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The problem is I have a surface mount ceramic chip capacitor exhibiting a
degradation in internal resistance but not so severe that it is readily
visible using light microscope and my SEM is down for repairs. Probable
cause is a microcrack and I need a suitable stain or dye penetrant to be
able to detect. If necessary I can penetrate using vacuum. I would
appreciate your resources and input on this problem. If there is a more
suitable forum....I do remember some mention of EDFAS which I plan to join
(Don't have time right now) please forward

Thanking you in advance

Robert Fowler
Quality Assurance Technician
TDK Components USA, Inc.
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com


THIS TRANSMISSION IS INTENDED FOR THE SOLE USE OF THE INDIVIDUAL AND ENTITY
TO WHOM IT IS ADDRESSED AND MAY CONTAIN PRIVILEGED AND/OR CONFIDENTIAL
INFORMATION.

If you are not the intended recipient, be advised that any use,
dissemination, distribution or duplication of this transmission is strictly
prohibited. If you received this transmission in error, please notify the
sender immediately by electronic reply to this transmission or by phone
(847-803-6100). Thank you.




From daemon Thu Aug 16 08:55:53 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Thu, 16 Aug 2001 09:44:07 -0400
Subject: RE: problem with JEOL T220A

Contents Retrieved from Microscopy Listserver Archives
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Dear Robert,

I experienced a similar problem a few years back with a JEOL 6300. As
ridiculous as it may sound, it turned out to be the nitrogen tank. It
simply ran out of gas. So, if you have a N2 tank attached to your scope,
check it. You may be in luck and just need to exchange the tank. Good
Luck!

Jackie Garfield
Electron Microscopist
LifeCell Corportation
One Millennium Way
Branchburg, NJ 08876



From daemon Fri Aug 17 06:06:59 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 16 Aug 2001 17:04:06 -0700
Subject: Hoya laser system help

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

We have a Wentworth LCS-2150 prober which is
fitted with a Hoya L212-62A laser head and a Hoya
L-210-P2 power supply. We do not have the user
manuals for this system and cannot make it work.
The laser fires but nothing happens to the chips
under the Mituyo objectives. Power supply is set
at 12KV. Simmer is completed but no pulse at
the target.

Does anyone have this Hoya system and associated
manuals? I would greatly appreciate a copy of any
documentation. Reproduction and shipping costs
will be gladly paid.

Thanks,
gary g.



From daemon Fri Aug 17 06:06:59 2001



From: max.sidorov-at-amd.com
Date: Thu, 16 Aug 2001 11:26:33 -0700
Subject: TEM/STEM query

Contents Retrieved from Microscopy Listserver Archives
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As far as I understand this instrument is intended to be for bio/medical
applications. In my experience, STEM doesn't have any serious advantage over
TEM for those applications (I don't have too much experience with bio
applications, though).

But by all means, if you have enough money, get TEM/STEM not just TEM. Most
of the popular TEM manufacturers give you STEM as an option. I wouldn't go
with a dedicated STEM in your case.
Here's what STEM migh give you:
1. You might get higher contrast in thicker samples than with TEM.
2. Z-contrast might be very helpful.
3. You can image with low dose and get decent images.
4. Extended analytical capabilities (you can conveniently collect all kinds
of signals but you need to have all kinds of detectors for that). Since you
are going to get a non FEG (I suppose) your resolution in STEM will probably
be not as good as in TEM.

The reason why your committee came up with the STEM suggestion might be
because, in fact, you can use a STEM in SEM mode. Just lower the voltage
down to 20kV or so and if you have an SE or BSE detector- you have an SEM.
So,

5. TEM/STEM can be put in SEM mode.

Hope this helps,

Max
__________________________________
Max Sidorov, Ph.D.
Advanced Micro Devices
Sunnyvale, CA



-----Original Message-----
} From: Richard Easingwood
[mailto:richard.easingwood-at-stonebow.otago.ac.nz]
Sent: Wednesday, August 08, 2001 2:18 PM
To: EM Listserver - messages
Cc: allan.mitchell-at-galadriel.otago.ac.nz;
bronwyn.smaill-at-galadriel.otago.ac.nz



Greetings all.

I have a small (actually not so small), but interesting problem which I
hope people out there in microscopy land may be able to help me with.

We have submitted two separate applications for replacement EM's. One
application was for a FEG-SEM with cold stage and the other for a 120kv
TEM with cryoholder. Both these configurations had been chosen after much
consultation with the researchers who use our Unit. The applications were
well supported by these researchers.

The response from our Equipment committee follows;

"Taking both applications into consideration, the committee would like to
ask you to examine the feasibility of applying for a scanning transmission
electron microscope (STEM), which the committee believe may better
facilitate research. Can you please investigate whether the purchase of
such an instrument would be a viable option for the University in enhancing
its biological science research ?"

We in the Unit believe that only a FESEM and a cryo-TEM will meet all the
needs expressed to us by researchers and that the purchase of a STEM will
mean spending a significant amount of money for a backward step in 'real
need' capability.

However I have to write a report justifying this belief.

Unfortunately I have no experience with STEM nor do I know a great deal
about STEM. As I see it a STEM with cryo-capability may well be a possible
option for our TEM researchers but my reason for not wishing to pursue the
STEM option is simply because the trade off would be that a significant
number of researchers at this University who have expressed a need for
access to a cryo-capable high resolution SEM to study frozen hydrated
'large' samples will not have their needs meet.
The purchase of a cryo-capable STEM will result in other compromises. For
example, I understand that to have a cryo-capable STEM means we would have
to compromise access to a back scattered electron detector. Due to design
considerations a cryo-capable STEM can not have a back scattered electron
detector fitted.

Any ideas to help me write my report will be gratefully received. Ideas
to the contrary of those expressed above are also very very welcome.

Replies please to Allan Mitchell, allan.mitchell-at-stonebow.otago.ac.nz.


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: office: 0064 3 479 7301
Mobile GSM: 0064 21 222 4759
Facsimile: 0064 3 479 7254
mailto:richard.easingwood-at-stonebow.otago.ac.nz
Web site: http://www.otago.ac.nz/anatomy/emunit/










From daemon Fri Aug 17 06:06:59 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 16 Aug 2001 15:26:35 -0500
Subject: Can't sputter nickel

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Hi Microlisters,

Recently I've been trying to sputter coat nickel onto biological samples for
x-ray microanalysis (EDS) in a SEM but have not been able to get any coating
under a variety of conditions onto a filter paper test sample. Gold works
fine.

Short digression: I like to use nickel because, compared to evaporated
carbon, it prevents charging more effectively, the K & L series nickel peaks
are at very low and high energies respectively, so the biological peaks of
interest are not overlapped, and you get better pictures of the analyzed
area with a metal coat on it. I used to coat from nickel wire in a vacuum
evaporator, but now have a sputter coater available on an SEM mounted
cryo-prep unit, so also want to coat Ni onto frozen hydrated biological
samples for EDS.

My unit is a D.C magnetron type. After reviewing the great discussions of
sputter coating that took place in this forum in January, 2001, I have a
hunch that i just don't have enough voltage in this unit to blast nickel
atoms off the target.

Any suggestions on what to try next?

Also, maybe I should revisit carbon coatings - is it possible to sputter
carbon without a special unit?

Thanks for any suggestions you might have,

Gib

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Aug 17 07:24:35 2001



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Thu, 16 Aug 2001 14:34:06 -0300
Subject: RE: LM of resin section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} Hi, All-
}
} It sure was nice to see so many of you at M&M 2001 in Long Beach last
} week!
}
} I generally only look at 0.5 to 2.0 micrometer epoxy or acrylic sections
} in the light microscope to see where in the block I am before taking
} ultrathin sections for TEM. I stain the thick sections with toluidine
} blue, glance at them and then throw them away. However, now I want to keep
} some of them and get good photomicrographs as well. In the past when I
} coverslipped them and took (35 mm film) pictures, they looked pretty
} bad. I've heard of various fixes, such as using thicker coverslips.
}
} Now I'd like to hear from someone doing this routinely, with good
} results (if possible!). What kind of mounting media? What kind of
} coverslips? Why? What goes on with the light as it passes through the
} resin?
}
} What kind of optics? Phase? Hoffman? DIC? Plain vanilla BF?
}
} Any clues will be appreciated!
}
} Aloha,
} Tina

Tina,

I use with full sucess semithin sections (embedded in Spurr resin) mounted
in Canada Balsam. However, it is important to remove the resin with a
saturated solution of NaOH in ethanol, during 5 minutes (for 0.35 µm) before
the staining procedures (Toluidine Blue or another technique). The
photomicrographs taked in DIC or BF microscopies are perfect for me.
I hope to have help you.


Dr. Rinaldo Pires dos Santos
Dept. of Botany - UFRGS
Rua Márcio Dias, 25 / apt. 305
Bairro Nonoai
90830-360 - Porto Alegre - RS
Brazil
E-mail: rinaldop-at-uol.com.br



From daemon Fri Aug 17 08:19:43 2001



From: coviello :      coviello-at-mae.uta.edu
Date: Thu, 16 Aug 2001 20:10:17 -0500
Subject: TEM: Encapsulating resin/polymer for materials science samples?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
For several years I have struggled with finding
the right encapsulant to encapsulate irregular shaped
samples, powders, etcetera that will stand up well to
heating, mechanical thinning, polishing and ion milling
(most bow when heated and have poor adhesion).
Does anyone out there(in Listserver Land) have any
recommendations of an encapsulant that they
use that has these type of characteristics? I would prefer
users experiences, rather than sales pitches...

While I am on the subject...does anyone have recommendations
of a protocol/procedure to process cross-sectional TEM specimens at
room temperature or pretty much near room temperature? eg room
temperature curing adhesives (to glue the samples together) and waxes
or adhesives to temporarily attach the sample to the polishing puck (I
have used Crazy Glue for this purpose, but it is not always reliable) to
make cross-sections of let's say a temperature sensitive device?
Materials science people will know what I am talking about (I hope).
Thanks for your help,
Regards,
Michael Coviello
Lab Manager
UT Arlington

P.S. Thanks Nestor for a great job and an extremely valuable resource



From daemon Fri Aug 17 08:24:40 2001



From: Gruber, Tyler :      tgruber-at-phelpsd.com
Date: Fri, 17 Aug 2001 09:18:37 -0400
Subject: Megaplus 1.4i to PC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Folks,

First-timer here.

We have an "old" Gatan Accuview 789 (based on Kodak Megaplus 1.4 or 1.4i, I'm not sure which) mounted on our TEM side (35mm?) port. We currently use it with a Mac. Sigh. We now want to have the capability to interface it to a PC. We do fairly simple imaging and limited image analysis (basic stuff). Can anyone tell me what we need? Easiest and/or cheapest route preferred. (Private replies to tcgruber1-at-excite.com are also welcome.)

TIA,

Tyler C. Gruber, Physicist III
Physics/Microscopy Laboratory
Columbian Chemicals Company



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Message-ID: {3B7C6EF8.E956E91E-at-mae.uta.edu}


Hi All:
For several years I have struggled with finding
the right encapsulant to encapsulate irregular shaped
samples, powders, etcetera that will stand up well to
heating, mechanical thinning, polishing and ion milling
(most bow when heated and have poor adhesion).
Does anyone out there(in Listserver Land) have any
recommendations of an encapsulant that they
use that has these type of characteristics? I would prefer
users experiences, rather than sales pitches...

While I am on the subject...does anyone have recommendations
of a protocol/procedure to process cross-sectional TEM specimens at
room temperature or pretty much near room temperature? eg room
temperature curing adhesives (to glue the samples together) and waxes
or adhesives to temporarily attach the sample to the polishing puck (I
have used Crazy Glue for this purpose, but it is not always reliable) to
make cross-sections of let's say a temperature sensitive device?
Materials science people will know what I am talking about (I hope).
Thanks for your help,
Regards,
Michael Coviello
Lab Manager
UT Arlington

P.S. Thanks Nestor for a great job and an extremely valuable resource



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Hi Folks,

First-timer here.

We have an "old" Gatan Accuview 789 (based on Kodak Megaplus 1.4 or 1.4i, I'm not sure which) mounted on our TEM side (35mm?) port. We currently use it with a Mac. Sigh. We now want to have the capability to interface it to a PC. We do fairly simple imaging and limited image analysis (basic stuff). Can anyone tell me what we need? Easiest and/or cheapest route preferred. (Private replies to tcgruber1-at-excite.com are also welcome.)

TIA,

Tyler C. Gruber, Physicist III
Physics/Microscopy Laboratory
Columbian Chemicals Company



From daemon Fri Aug 17 08:52:33 2001



From: Henderson, Jack D :      jhenderson-at-atd.crane.navy.mil
Date: Fri, 17 Aug 2001 08:36:24 -0500
Subject: RE: Semiconductor Contaminants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An older article by Bob Lowry, et al., at Harris (now Intersil) has images,
elemental composition data, and interesting discussion of human contaminants
on IC's.

"Analysis of Human Contaminants Pinpoint Sources of IC Defects"
Lowry, R.K., Linn, J.H., Grove, G.M., and Vicroy, C.A.
Semiconductor International, v 10 n 8 July 1987 p 73-77

Jack D. Henderson
Naval Surface Warfare Center Crane Division


} -----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-anadigics.com]
} Sent: Wednesday, August 15, 2001 6:56 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Semiconductor Contaminants
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone out there know of a good source, text or website,
} that deals
} with the issue of contaminants in a semiconductor
} manufacturing environment?
} I'm putting together a lecture on the subject but I don't
} want to generate
} SEM's, optical images etc. if this is available readily.
} Principally I'm
} interested in human spittle, airborne contaminates such as
} skin, hair, cloth
} fibers, etc. or anything that may be generated by human
} interaction with a
} semiconductor device.
}
} Regards,
} Peter Tomic
} Anadigics, Inc.
}


From daemon Fri Aug 17 08:59:03 2001



From: Dave Roberts :      dave-at-boeckeler.com
Date: Fri, 17 Aug 2001 08:55:26 -0500
Subject: M&M meeting: Digital Camera winner

Contents Retrieved from Microscopy Listserver Archives
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------=_NextPart_000_0000_01C1266C.2D329E20
Content-Type: text/plain; charset="iso-8859-1"
X-Sun-Content-Length: 675

Many thanks to everyone who visited the RMC-Boeckeler Instruments booth and
special thanks to those of you who took the time to fill out our survey and
enter the drawing for a digital camera.

I am pleased to announce that the lucky winner is Mr. Jon Charlesworth at
Mayo Clinic. Good luck Jon and hope you have lots of fun with your new
"toy".

Sorry that you couldn't all be winners but better luck next time.
See you in Quebec and be sure to visit our booth to try for next year's
drawing.

Dave Roberts
Director- RMC EM Products
Boeckeler Instruments, Inc.
4650 S. Butterfield Drive
Tucson, AZ 85714
Tel: (520) 745-0001 Fax: (520) 745-0004
website: www.rmcproducts.com


------=_NextPart_000_0000_01C1266C.2D329E20
Content-Type: text/html; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
X-Sun-Content-Length: 2366

{!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"}
Many = thanks to=20 everyone who visited the RMC-Boeckeler
Instruments booth and special = thanks to=20 those of you who took
the time to fill out our survey and enter the = drawing for=20 a
digital camera.

I am = pleased to=20 announce that the lucky winner is Mr. Jon
Charlesworth at Mayo = Clinic. =20 Good luck Jon and hope you have
lots of fun with your new=20 "toy".

Sorry = that you=20 couldn't all be winners but better luck next time.
See = you in Quebec=20 and be sure to visit our booth to try for next
year's=20 drawing.

Dave=20 Roberts
Director- RMC EM=20 Products
Boeckeler=20 Instruments, Inc.
4650 = S. Butterfield=20 Drive
Tucson, AZ=20 85714
Tel: = (520) 745-0001=20 Fax: (520) 745-0004
website: {http://www.rmcproducts.com"} www.rmcproducts.com=



------=_NextPart_000_0000_01C1266C.2D329E20--


From daemon Fri Aug 17 09:17:38 2001



From: zaluzec-at-microscopy.com
Date: Fri, 17 Aug 2001 09:14:18 -0500
Subject: Administrivia: Network Feed was down

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry Folks...

To all you that asked, no it's not a problem with your Email account
or your subscription.

The TelCo ( AT&T ) connnection to my router was down for nearly 24 hours.

The network simply failed. Somewhere between Chicago and here. For
the moment all appears to be working but they are still doing testing.
Probably some small animal chewing on buried cable or something
equally silly, but hard to find.

Hope you all enjoyed the quiet day.

Nestor
Your Friendly Neighborhood SysOp


From daemon Fri Aug 17 09:44:40 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Aug 2001 07:40:36 -0700
Subject: Hoya laser system help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

We have a Wentworth LCS-2150 prober which is
fitted with a Hoya L212-62A laser head and a Hoya
L-210-P2 power supply. We do not have the user
manuals for this system and cannot make it work.
The laser fires but nothing happens to the chips
under the Mituyo objectives. Power supply is set
at 12KV. Simmer is completed but no pulse at
the target.

Does anyone have this Hoya system and associated
manuals? I would greatly appreciate a copy of any
documentation. Reproduction and shipping costs
will be gladly paid.

Thanks,
gary g.



From daemon Fri Aug 17 10:55:12 2001



From: Caroline Miller :      camiller-at-creighton.edu
Date: Fri, 17 Aug 2001 10:49:32 -0500
Subject: DAB Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm interested in protocols using DAB, what type of fixation, embedding
media, etc. Caroline Miller



From daemon Fri Aug 17 12:33:48 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 17 Aug 2001 12:29:35 -0500
Subject: Facility support survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Micro listers,

This is a survey that was passed out at the Facility Managers Meeting
at this year's M&M meeting. We've only gotten 6 responses so far, and
many more are needed for valid results (although obviously only one
answer per facility). Please take a couple of minutes to fill this
form in, and email back to me. Thank you!

Phil

MICROSCOPY FACILITY SUPPORT

This survey will help compile the nature and amount of support
provided to microscopy facilities by universities, colleges, and
research institutes. We would like to determine how much facility
support is provided by the institution, and how much must be
generated by user fees or other sources, on a per cent basis.

If we get enough responses to have valid data, we will post the
results on the microscopy listserver and send them to the MSA
bulletin.
Names of institutions will not be included in the reported
results.

Institution:
Location:
Nature of institution (Academic, Commercial, private research, etc.)

Type of Facility (institution core, satellite, departmental, etc):

(Place an 'X' by the appropriate answer)
Predominately:
Biological
Materials
Both

User base:
Service
Multi-user
Both

Type of microscopes:
SEM
FESEM
ESEM or LV
TEM
FETEM
Major optical (Specify)
Scanning Probe (specify)
Other (specify)

Approximately what *per cent* of the funds for each of the following
is provided by
A) your institution, B) user fees, or C) grants

Salaries:
A)
B)
C)

Instrument Maintenance (Service contracts, instrument insurance, etc):
A)
B)
C)

Replacement of existing equipment:
A)
B)
C)

Purchase of new equipment (not replacement for but addition to
existing equipment):
A)
B)
C)

Supplies:
A)
B)
C)

Other operating expenses:
A)
B)
C)

Are there other microscope facilities at your institution? if
so, please indicate approximate number having electron
microscopes. Ideally we would like a form filled out for each
major facility (multiple instruments) at your institution if
there are more than one.

Please include additional information such as novel funding
ideas for your facility or for others at your institution.

Please email your responses to Philip Oshel
peoshel-at-facstaff.wisc.edu
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Aug 17 13:09:18 2001



From: =?iso-8859-1?q?Ike=20Oguocha?= :      oguocha-at-yahoo.com
Date: Fri, 17 Aug 2001 19:03:48 +0100 (BST)
Subject: Choosing an Analytical SEM for Metallurgical Research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Netters:

In about three weeks time or so, I'll be writing equipment grant
appilcations. Our lab needs, as a matter of necessity, an analytical
SEM. The one we have now is relatively old (Philips SEM515) and
has no EDS facilities. It hinders most of our work since we have
to go elsewhere and pay for any work involving EDS. Our attempt
last year to get a EDS system grant for it failed. So, we are
making a completely new equipment grant this time.

I am wondering if there are metallurgical microscopists in the
house to give me some insights on which direction to go. What
must we be looking for in a good analytical SEM? Who makes what?

Many thanks in advance.

Ike Oguocha
Department of Mechanical Engineering
University of Saskatchewan
Saskatoon, Canada


____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie


From daemon Fri Aug 17 14:12:39 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 17 Aug 2001 15:49:36 -0400
Subject: cell attachment to coverslips or aclar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student who's had little success attaching and then
retaining neurons and PC12 cells to coverslips (glass, Thermanox and
aclar) in an attempt to use a pre-embedd labeling procedure. She has
coated glass with collagen for the PC12 culture. Any suggestions
will be appreciated.
Rosemary
--
Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html


From daemon Fri Aug 17 14:33:54 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 17 Aug 2001 15:24:32 -0400
Subject: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 3:53 PM -0500 8/8/01, John J. Turek wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have a question from a client that is beyond my area of expertise,
so I turn to you for help.

He would like to look at the layer of ink deposited on paper by the
traditional (Guttenberg type) printing press in order to help him
standardize his printing process. I haven't a clue as to how to
approach this. His idea was to look in SEM since his understanding
is that the ink layer is, on average, 4 micrometers thick , but my
feeling is that the ink will have absorbed into the paper and not
present any topography.

Any ideas? I have TEM & SEM at my disposal, as well as CLSM and widefield LM.

Thanks in advance,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Aug 17 14:42:28 2001



From: Alexander H. King :      alexking-at-ecn.purdue.edu
Date: Fri, 17 Aug 2001 14:37:44 -0500
Subject: SEM/TEM Technician Position Available - Purdue University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




POSITION ANNOUNCEMENT

Electron Microscope Specialist

Purdue University seeks an Electron Microscope Specialist to provide
service and support for the scanning and transmission electron microscopes,
and related equipment, managed by the Purdue Electron Microscopy
Consortium. Serving as a primary point-of-contact for all electron
microscopy related concerns, questions and problems. He/she will manage
the day-to-day maintenance of our equipment. He/she will also interface
with manufacturers, provide training to users, train and supervise interns,
and assist in the evaluation of proposed and existing equipment.

The successful candidate will possess at least an associates degree (or
equivalent) in science, engineering or technology, suitable training in
electronics and vacuum systems, and significant experience in the
maintenance of electron microscopes and associated equipment.

This position will remain open until filled. For full consideration,
applications should be received by October 31, 2001.

The Purdue Electron Microscopy Consortium oversees and co-ordinates the
operation of nearly 30 electron microscopes (organized in four clusters)
that support the educational and research missions of the university.

Purdue offers competitive salaries and an outstanding benefit
package. Located in West Lafayette, Indiana, it provides a very attractive
environment in which to live and work.

Applicants should send a letter of interest and a detailed CV to:

Prof. Alex King
Director, Purdue Electron Microscopy Consortium
School of Materials Engineering
Purdue University
West Lafayette IN 47907-1289

alexking-at-ecn.purdue.edu

Purdue is an affirmative action / equal opportunity employer. Women and
minorities are encouraged to apply.




From daemon Fri Aug 17 15:00:25 2001



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Sat, 18 Aug 2001 15:57:25 -0400
Subject: CRT repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

I recall a couple postings several months ago pertaining to old CRTs that
were in need of repair. There were a couple responses with names of someone
who repairs these old CRTs. I swore I printed those off but am unable to
find it. If anyone has that info I would appreciate hearing from you. Thanks.

Joel McClintock
EM Specialist
U of Kentucky
859-257-1242



From daemon Fri Aug 17 15:27:28 2001



From: Paul B. Grover :      pbgrover-at-home.com
Date: Fri, 17 Aug 2001 15:23:15 -0700
Subject: Earnest Fullam sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
}
} I'm considering buying a used Sputter Coater originally marketed by Earnest
} Fullam (I'm on a real shoestring budget). A web search doesn't turn up any
} results for an extant company of that name.
}
} Anyone know whether the company still exists, and/or whether replacement
} parts are available for such a unit? Thanks for any info.
}
} Cheers,
}
} Paul Grover :o)



From daemon Fri Aug 17 16:11:42 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 17 Aug 2001 17:06:50 -0400
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






I have a question from a client that is beyond my area of expertise,
so I turn to you for help.

He would like to look at the layer of ink deposited on paper by the
traditional (Guttenberg type) printing press in order to help him
standardize his printing process. I haven't a clue as to how to
approach this. His idea was to look in SEM since his understanding
is that the ink layer is, on average, 4 micrometers thick , but my
feeling is that the ink will have absorbed into the paper and not
present any topography.

Any ideas? I have TEM & SEM at my disposal, as well as CLSM and widefield
LM.

Dear Lee,
What is the composition of the ink? If it has element(s) not present
in the paper, perhaps you could use XMA or EELS to define the regions of
the paper where the ink is. Cutting cross-sections perpendicular to the
plane of the paper may give you enough spatial resolution, or you could try
to prepare individual fibers if there is a technique to do this without
redistributing the ink. This last method would give info about how far the
ink gets into each fiber, but you would then have to find out how the
fibers are oriented in the paper to get the ink distribution as a function
of distance from the surface. This could be a difficult problem to
unravel.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Aug 17 20:34:59 2001



From: :      secretmail-at-myplace.com
Date: Fri, 17 Aug 2001 21:28:04 -0400 (EDT)
Subject: SOLICITING FOR YOUR ASSISTANCE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FROM: CHIEF MRS. MARYAM ABACHA,
ABACHA VILLA, KANO-NIGERIA.
Dear Sir/Madam

Thank you for taking the pain to go through my mail. My name is CHIEF
MRS. MARYAM ABACHA. I would first like to apologise for this kind of
introduction into your mailbox, but the situation I am now did not
afford me any better way.
It is with a heart full of hope that I write to seek your help in the
context below. I am the wife of the former Nigerian Head of State
late General Sani Abacha whose sudden death occurred on the 8th of
June 1998.
Having gotten your contact from the family's Library I have no doubt
about your capability and goodwill to assist me in receiving into
your custody (for safety). The sum of TWENTY ONE MILLION, SEVEN
HUNDRED THOUSAND UNITED STATES DOLLARS (US$21.7 MILLION) WILLED and
deposited in my favour by my late husband. The money is currently
kept in a Trust deposited Account with one of the private Security
Companies.
As it is legally required, the Administration of my late husband is
under the authority of the family's lawyer named (Mark Mercyke).
However, the new Democratic Government has an assumption of office
set up a panel of inquiry to probe the financial activities of my
late husband (Former Head of State) with a decision to freeze all his
assets respectively. The investigative team has submitted their
report. Presently, some Bank Accounts and valuable assets have been
frozen and seized.
Fortunately, our family lawyer had secretly advised that the WILLED
money be urgently moved into an unknown overseas Bank account of a
trusted person or company without delay for security reasons. The
Government had earlier placed an embargo on Abacha's family members
from traveling outside the Country. My son (Mohammed Abacha) is in
prison custody being tried for a crime he is innocent of . The
situation has been so terrible that we are virtually. I expect you to
be trustworthy and kind enough to respond to this call (S.O.S) to
save me and my children from a hopeless future.
I hereby agree with the Lawyer to compensate you with 25% of the
total sum (US$21.7Million) for your sincere and candid effort when
the money is finally received into your Bank Account. We have also
set aside 5% of the total sum for settling any expenses you and I
might incur during the process of the transfer of this money. That is
to say, any amount you spend e.g. Telephone Bills, Security Charges
etc. must be refunded to you before the sharing of the money and the
same thing to us.The lawyer and the Security Company have already
perfected
arrangement with the manners to effect complete dislodgement of this
money within 7 days of receipt of your response. They have equally
guaranteed 100% RISK FREE and smooth transfer. Please, send the
following information to the Lawyer immediately.
(1) YOUR FULL NAME
(2) YOUR ADDRESS
(3) YOUR PHONE AND FAX NUMBERS.
All CORRESPONDENCE/INQUIRY MUST BE DIRECTED TO THE LAWYER VIA THE E-
MAIL ADDRESS:markmercyke-at-lawyer.com, as we count on your
trustworthiness
and confidentiality. Thanks for your kind attention.
I look forward to your quick response.
With Best Regards.
Chief Mrs Maryam Abacha.

http://www.myplace.com/ - find what you want -at-MyPlace.com!


From daemon Fri Aug 17 21:19:16 2001



From: Paul B. Grover :      pbgrover-at-home.com
Date: Fri, 17 Aug 2001 21:15:54 -0700
Subject: The importance of being Ernest*

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


(*with apologies to Oscar Wilde)

Thank you to all who have spotted my bad spellling (I plead innocence, as
the advertisement had the spelling 'Earnest Fullam'). I'm glad so many are
on vacation so that they don't see the egg on my face.

Paul Grover :o}



From daemon Sat Aug 18 02:07:42 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Sat, 18 Aug 2001 02:01:41 -0500
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}

} Hello Netters,
}
} I have a question from a client that is beyond my area of expertise,
} so I turn to you for help.
}
} He would like to look at the layer of ink deposited on paper by the
} traditional (Guttenberg type) printing press in order to help him
} standardize his printing process. I haven't a clue as to how to
} approach this. His idea was to look in SEM since his understanding
} is that the ink layer is, on average, 4 micrometers thick , but my
} feeling is that the ink will have absorbed into the paper and not
} present any topography.
}
} Any ideas? I have TEM & SEM at my disposal, as well as CLSM and widefield
LM.
}
} Thanks in advance,
} Lee

Depending on the coating on the paper and the composition of the ink the ink
will be to a mostly deposited on the surface of the paper. Actually very
little will be absorbed into the paper or you would have bleed through like
that you see when you use Magic Markers or regular paper. It would also
cause the type to bleed and become unsharp like you see when you use the
wrong type of paper in an ink jet printer.

Printers ink is a compound of pigments, oils and dryers that that forms a
polymer much like oil paint on paper. Very little of the drying it due to
evaporation of solvents or the absorption of solvents by the paper. The ink
is put in place and left to dry by transfer from the type to paper by very
firm pressure.

Printing is just one of many things I learned to do in my life that
technology has made obsolete.

Your customer knows what he is talking about.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger






From daemon Sat Aug 18 09:40:35 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 18 Aug 2001 09:11:41 -0700
Subject: Re: Earnest Fullam sputter coater

Contents Retrieved from Microscopy Listserver Archives
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Wonderful!

Here is the information of my EX-wife:


Regards,

Earl


----- Original Message -----
} From: {secretmail-at-myplace.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 17, 2001 6:28 PM


Try this URL for their home page:

http://www.fullam.com/

This one for their sputter coater:

http://www.fullam.com/Effacoat.htm#18930

gary g.


At 03:23 PM 8/17/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Aug 18 11:16:58 2001



From: Edwards Forensic Service :      fedwards-at-mninter.net
Date: Sat, 18 Aug 2001 11:14:28 -0600
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Lee:

Try a lower tech approach:

1. Weigh a piece of paper in an analytical balance.
2. Print on it and let it dry.
3. Weigh it again.
4. Use your flatbed scanner to scan the image.
5. Save as a black and white image.
6. Use an image processing program to tell what percent of the surface
is black, ie. inked. (Many scanners come with software that allows this
type of simple image processing.)
7. Do the math.

If you need to know what your error is do a number of these measurements.

Your error is probably far less than with the TEM.

Harold Edwards




From daemon Sat Aug 18 13:37:51 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Sat, 18 Aug 2001 11:30:16 -0700 (PDT)
Subject: Re: cell attachment to coverslips or aclar

Contents Retrieved from Microscopy Listserver Archives
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We use a solution of 5 % gelatin in distilled water to attach cells to
glass surface. It has to be diluted at 70 ºC on a magnetic shaker. Then
the glasses are dept and dryed. It works.

Albert Cardona
University of Barcelona

__________________________________________________
Do You Yahoo!?
Make international calls for as low as $.04/minute with Yahoo! Messenger
http://phonecard.yahoo.com/


From daemon Sat Aug 18 13:46:32 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Sat, 18 Aug 2001 11:41:16 -0700 (PDT)
Subject: Re: DAB Staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Caroline

Fixation: paraformaldehid 4% (merck)
pepsin (sigma) 0,4 % in HCl 0,1 N pH 5
milk
primary antibody
biotinilated secondary antibody (sigma)
avidin + biotin-peroxidase complex (you can get both apart in Sigma
too)
DAB revealing solution: DAB 0,3 % in PBS
niquel amonium sulfate 0,2
%
water peroxide 0,03 %
-in PBS.

There can be found recipes on the net. Just write the name of any of
the components on google or yahoo.

Albert Cardona
University of Barcelona

__________________________________________________
Do You Yahoo!?
Make international calls for as low as $.04/minute with Yahoo! Messenger
http://phonecard.yahoo.com/


From daemon Sat Aug 18 18:50:12 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 18 Aug 2001 16:44:04 -0700
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An interesting question and problem. I shot the following
images using SEM:

ftp://www.gaugler.com

laserpaper-2.tif
laserpaper-3.tif

printedpaper-2.tif
printedpaper-4mix.tif
printedpaper-6bse.tif

These were from my HP LaserJet 4 (laserpaper) and from page 61
of the Volume 7, Supplement 1 issue of Microscopy
and Microanalysis, lower right corner of page 75,
grabbing the word "Irvine" (printedpaper).

I did SE and BSE and SE+BSE of these specimens.
It is interesting that the laser images turn out quite good.
Not so for the offset printing. They are basically non-visible.

However, I did a series of shots using Zeiss DIC LM and
can see both imprints very well. In either case, I do not
see any absorption of the laser particles or the printing ink.

If anyone is interested, I can put digital images from the LM
scope on the site as well. I do seem to conclude that LM
observation is more useful than SEM. I would think the same
about TEM.

gary g.




At 12:24 PM 8/17/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Aug 18 19:27:48 2001



From: Cat-at-neto.com ()
Date: Sat, 18 Aug 2001 19:23:47 -0500
Subject: Ask-A-Microscopist: compound microscope magnification

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Cat-at-neto.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
August 18, 2001 at 17:24:17
---------------------------------------------------------------------------

Email: Cat-at-neto.com
Name: Jeni Hall

Organization: Alpha-Omega Home School

Education: 9-12th Grade High School

Location: Paris, Texas America

Question: If a compound microscope has a ten-power eye piece and a
ninety-power objective lens, what is its magnification power? Also,
what is the limitation of an optical microscope?

---------------------------------------------------------------------------


From daemon Sun Aug 19 11:30:41 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 19 Aug 2001 09:13:24 -0700
Subject: Re: Ask-A-Microscopist: compound microscope magnification

Contents Retrieved from Microscopy Listserver Archives
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} Organization: Alpha-Omega Home School
}
} Education: 9-12th Grade High School
}
} Location: Paris, Texas America
}
} Question: If a compound microscope has a ten-power eye piece and a
} ninety-power objective lens, what is its magnification power? Also,
} what is the limitation of an optical microscope?
}
} ---------------------------------------------------------------------------

Jeni -

10x90=900. And if that is a calculation for your microscope, please be
aware that any objctive of that power is going to provide a very poor image
unless it is designed for use with immersion oil. You'll find a lot of
well- organized,useful background information on microscope optics on the
Florida State University's "Molecular Expressions" website,
http://microscopy.fsu.edu/optics/index.html


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Aug 19 13:55:21 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 19 Aug 2001 11:49:11 -0700
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
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Tobias Baskin pointed out the probable difference between
Gutenberg printing and modern web printing.

I was wondering about that too. Gutenberg. Heard about it
but not certain what it actually is. I suspect the magazine is
indeed offset web press. But if the the two processes
are different, are they both based on ink of the same type?
If so, I can't see it in the SEM either SE or BSE. But sure
can under LM.

I suspect that based on the variety of old books I have,
there may or may not be a build up of ink on the paper.
Some paper is coated stock whereas others are quite
porous. As I think about this, I think the reason I cannot
"see" the web printing ink is because it is more "pushed"
into the paper. It is akin to being absorbed by the paper.
The laser toner is distinctly different and is laying on top
of the paper. The laser fuser heats up the toner particles
(readily visible as such) and melts and sticks the particles
to the paper rather than having the paper absorb them.
SEM imaging of the web printing reveals nothing but the
texture of the paper fibers. The ink is embedded but
is difficult to visualize using SE or BSE.

If I knew the age of Gutenberg type of printing, I do have
some rather old books around. Some are late 1700's up
to early 1900's. A small piece of one could
be sacrificed in the name of science!

I'm working on thin layers of Au on organic substrates
for doing ultrasonic imaging in catheters. I find some
similarities between this and the ink question. Quite
interesting and useful.


Regarding the pix at my web site--
JPEGs of same root name are at the site too. These are
only about 45KB-80KB each. These may be better to access since
some browsers use Quicktime to display TIFF whereas
IE for example will directly display JPEG. In this case,
just grab them via http://www.gaugler.com/name.jpg

For example, http://www.gaugler.com/laserpaper-2.jpg
All image files are sized to be 450pixels wide.

I also added two files:
printedpaper6-bsel.tif
printedpaper6-bsel.jpg

which are TIFF and JPEG images of printedpaper6-bse
after processing with Image Content's Lucis. I'm certainly
no paper expert, but it looks like with web printing, the
ink is absorbed into the paper's fibers. The lower right
appears to be a glob of ink. What is puzzling is that
under DIC LM, both laser and web printing have three
dimensional aspects. This is not seen in SEM for
both types of printing. Perhaps the Gutenberg method
will indeed show up.

gary g.



From daemon Sun Aug 19 15:53:25 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Sun, 19 Aug 2001 15:46:00 -0500
Subject: Re: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
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Gary (and any others),

Stop! Pleae don't cut up any of your old books!

Moveable type was used routinely for printing from
Gutenburg's invention in 1450 or so until after the second war. But
even today there are many printers around who use it for small
editions and other kinds of "handmade" printing projects (just as
there are potters who still throw pots by hand, etc). So if anyone
out there in net land is stimulated by this thread to want to image
some letter press printing, you can almost certainly find a printer
in your area who can give you some scraps to examine. No need
whatever to cut up old books.
The same action as printing from type, that is where you have
metal in a frame that is inked and then a paper is pressed onto it
(where the word "press" comes from) is found in other kinds of
printing. I think in some methods photography plus some sort of
etching is used to make the metal template. This is all contrasted to
methods like the "xerox" where charge is used to put the ink to the
paper (the paper in a xerox machine never gets pressed to any
extent), and to methods like ink- and laser-jets where the ink is
sprayed onto the paper. Again, no pressure.
It is clear that the kind of ink you need to lay nicely on a
raised metal surface and be pressed onto the page is different than
the kind of ink you need to go through a nozzle or be transferred by
charge. I can remember from hanging around my dad's letterpress
office, the ink came in tins and had the consistancy of chocolate
frosting. One used a putty knife to spot a glop on a glass surface
and then a roller to roll it out smooth and then apply to the type.
If you were printing by hand you did this after every impression--an
automated press had ususally a few dozen rollers to spread the ink
evenly. Anyway, I think some of these "printer's inks" are colloids
of metal particles (like good old titanium oxide white house paint),
so possibly imageable in the SEM, though I am not arguing against
using light. (And for sure, the idea of weighing the printed output
seemed excellent from the point of view of standardizing the inking
quantity).

Hope this helps,
Tobias Baskin


--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Sun Aug 19 21:34:49 2001



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Mon, 20 Aug 2001 12:23:46 +1000
Subject: Resin embedding of porous coal chars for SEM

Contents Retrieved from Microscopy Listserver Archives
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G'day

I have some students who want to examine cross sections of very porous
coal char in the SEM. Trying to get bubble free blocks is proving difficult
as the particles tend to float in the resin (so I am told) and it is difficult to
get good impregnation. Even recoating the ground surface and repolishing
leaves an unacceptable amount of bubbles in the surface and in the hollow
particles. Has anyone perfected a technique for similar samples and be
willing to share it? We usually have no problems with polished blocks, it is
just this material!

Thanks

Dave




Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au



From daemon Mon Aug 20 01:42:38 2001



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Mon, 20 Aug 2001 08:31:08 +0200
Subject: Re: LM of resin section

Contents Retrieved from Microscopy Listserver Archives
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Tina
You could try making semi-permanent mounts (as long as they are stored
in horizontal position) by mounting Tol blue-stained semithin sections
(0.5 to about 2micrometers thick) in a drop of immersion oil. The
refractive index of the oil (1.515 or thereabouts) matches that of the
resin quite closely, with the result that knife marks etc disappear.

If the sections are too dry before they are mounted, the
metachromicity of the Tol blue disappears, and the sections appear
uniformly blue. You could try the old trick of breathing on 50deg C
dried sections to slightly hydrate them before mounting in the oil.
Residual liquid water in the sections is undesirable and shows up as
very visible droplets and unwetted (with oil) areas.

It becomes slightly messy if you need to look at the slides with
immersion optics since the coverslip then has oil below and on top.
In this case you can easily remove the coverslip without problems
(gently slide it off sideways), and look at the sections in oil
without the coverslip.

The stain seems to last reasonably well in the oil. I don't have
experimental data, but some old (few years at least) mounts still look
good. The only problem is that the slides have to be stored flat.

Try it, I think you'll like it.

Regards
Jan


Rinaldo Pires dos Santos wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Tina Carvalho wrote:
}
} } Hi, All-
} }
} } It sure was nice to see so many of you at M&M 2001 in Long Beach last
} } week!
} }
} } I generally only look at 0.5 to 2.0 micrometer epoxy or acrylic sections
} } in the light microscope to see where in the block I am before taking
} } ultrathin sections for TEM. I stain the thick sections with toluidine
} } blue, glance at them and then throw them away. However, now I want to keep
} } some of them and get good photomicrographs as well. In the past when I
} } coverslipped them and took (35 mm film) pictures, they looked pretty
} } bad. I've heard of various fixes, such as using thicker coverslips.
} }
} } Now I'd like to hear from someone doing this routinely, with good
} } results (if possible!). What kind of mounting media? What kind of
} } coverslips? Why? What goes on with the light as it passes through the
} } resin?
} }
} } What kind of optics? Phase? Hoffman? DIC? Plain vanilla BF?
} }
} } Any clues will be appreciated!
} }
} } Aloha,
} } Tina
}
} Tina,
}
} I use with full sucess semithin sections (embedded in Spurr resin) mounted
} in Canada Balsam. However, it is important to remove the resin with a
} saturated solution of NaOH in ethanol, during 5 minutes (for 0.35 µm) before
} the staining procedures (Toluidine Blue or another technique). The
} photomicrographs taked in DIC or BF microscopies are perfect for me.
} I hope to have help you.
}
} Dr. Rinaldo Pires dos Santos
} Dept. of Botany - UFRGS
} Rua Márcio Dias, 25 / apt. 305
} Bairro Nonoai
} 90830-360 - Porto Alegre - RS
} Brazil
} E-mail: rinaldop-at-uol.com.br

--
Prof Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/academic/electron/emunit1.htm


From daemon Mon Aug 20 07:57:45 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Mon, 20 Aug 2001 08:49:30 -0400
Subject: FW: Resin embedding of porous coal chars for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dave, Do you have access to a centrifuge somewhere on campus? If your
samples are indeed porous and have sufficient density you could try to
centrifuge the samples in your embedding media, preferably something with
low viscosity. While I have never tried this, it may work.
G'luk,
Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com





G'day

I have some students who want to examine cross sections of very porous
coal char in the SEM. Trying to get bubble free blocks is proving difficult
as the particles tend to float in the resin (so I am told) and it is
difficult to
get good impregnation. Even recoating the ground surface and repolishing
leaves an unacceptable amount of bubbles in the surface and in the hollow
particles. Has anyone perfected a technique for similar samples and be
willing to share it? We usually have no problems with polished blocks, it is

just this material!

Thanks

Dave




Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au



From daemon Mon Aug 20 09:24:51 2001



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Mon, 20 Aug 2001 10:17:04 -0400
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Mon Aug 20 10:01:59 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 20 Aug 2001 10:55:28 -0400
Subject: RE: TEM of paper & ink

Contents Retrieved from Microscopy Listserver Archives
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Apparently Gutenberg use a proprietary ink (graphite + linseed oil +
cooking? of some sort). Not surprising that it wouldn't do too well in SEM.
Too much C! LM sounds good to me too. For analysis, however, it might be
useful to 'dope' the ink for the study (scientific?) phase. Little
colloidal gold,
or silver,
or osmi___um.
Ought to be a song there somewhere.

Regards and happy to hear you all had such a good time,

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
CASI Home Page: http://darwin.wcupa.edu/casi/
Schedule: http://darwin.wcupa.edu/cgi-bin/casireserve.cgi
Please call before visiting.


} ----------
} From: Leona Cohen-Gould
} Sent: Friday, August 17, 2001 3:24 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM of paper & ink
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 3:53 PM -0500 8/8/01, John J. Turek wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} Hello Netters,
}
} I have a question from a client that is beyond my area of expertise,
} so I turn to you for help.
}
} He would like to look at the layer of ink deposited on paper by the
} traditional (Guttenberg type) printing press in order to help him
} standardize his printing process. I haven't a clue as to how to
} approach this. His idea was to look in SEM since his understanding
} is that the ink layer is, on average, 4 micrometers thick , but my
} feeling is that the ink will have absorbed into the paper and not
} present any topography.
}
} Any ideas? I have TEM & SEM at my disposal, as well as CLSM and widefield
} LM.
}
} Thanks in advance,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}


From daemon Mon Aug 20 11:08:25 2001



From: coviello :      coviello-at-mae.uta.edu
Date: Sun, 19 Aug 2001 22:59:53 -0500
Subject: TEM-Looking for a used dualbeam FIB and TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi All:
We are starting to look for an inexpensive, used FIB to use
for materials science applications. Are there any recommendations
as to where to look? Also looking for an older 200 Kv or higher
TEM in good working condition. Any recommendations?
Mike
UT Arlington



From daemon Mon Aug 20 11:09:06 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 20 Aug 2001 09:02:25 -0700
Subject: Re: Resin embedding of porous coal chars for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dave,
I have had some success with particles of this type. First, mix a small
amount of resin and the powder thoroughly together in a small weigh boat,
so that the powder is well wetted. Pour this into the bottom of your
(lightly release-sprayed) mold and then carefully trickle clear resin on top
of this, so your coal/resin layer stays on the bottom. You might want to try
pulling a vacuum on the weigh boat sample before pouring it into the mold,
as well as after all the resin is in the mold.
At 12:23 PM 8/20/01 +1000, you wrote:
} G'day
}
} I have some students who want to examine cross sections of very porous
} coal char in the SEM. Trying to get bubble free blocks is proving difficult
} as the particles tend to float in the resin (so I am told) and it is
difficult to
} get good impregnation. Even recoating the ground surface and repolishing
} leaves an unacceptable amount of bubbles in the surface and in the hollow
} particles. Has anyone perfected a technique for similar samples and be
} willing to share it? We usually have no problems with polished blocks, it is
} just this material!
}
} Thanks
}
} Dave
}
Regards and good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Aug 20 11:33:22 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Mon, 20 Aug 2001 12:28:13 -0400
Subject: LM - Something I just don't get

Contents Retrieved from Microscopy Listserver Archives
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Numerical aperture/magnification/image brightness

Why does numerical aperture increase with magnification, rather than
decrease? In fact, why is there a relationship between magnification and
numerical aperture at all? If increased numerical aperture means better
light gathering ability, that implies that higher magnification objectives
should produce brighter images.

This, to me, is like saying that going from a 50mm camera lens to a 1000mm
lens is going to give me more light. In the photographic world, increased
light gathering ability comes only from increased lens diameter and it is
completely independent of the "magnification" of the lens.

I visited the Nikon N.A. Java tutorial at
http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
and this only seemed to reinforce the discrepancy for me.

With an SEM I can imagine a scan over a smaller area (a tighter scan)
producing an increase in magnification. In a photographic system I can
envision viewing a smaller ray bundle (smaller solid angle) producing a
larger magnification. How is it that in light microscopy this is reversed
and a high magnification objective takes in a larger angle? What is backward
in my thinking?

Bruce Girrell
Microline Technology Corp.
2397 Traversefield Dr.
Traverse City, MI 49686
http://www.microlinetc.com

(231) 935-1585 (Voice)
(231) 922-5099 (FAX)
bigirrell-at-microlinetc.com



From daemon Mon Aug 20 13:49:00 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 20 Aug 2001 14:35:35 -0400
Subject: Re: TEM of paper & ink

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Hi All,

thank you for your rapid and varied responses to my question. I
think I will try the weighing trick as a quick way to get some sort
of answer for this guy. Printing is his hobby, so this is not
work-related, and I think he came in only because the traffic flow in
the building has been redirected to pass my doors during renovation
of the main entrance, and he wanted to see what an EM looks like and
get a shot at playing on it. I've had to put new, coded locks on my
doors because of all the curious people just wandering in! It seemed
to be an interesting problem, but not one I'm willing or able to put
much time/energy too. I was hoping for answers that would contain
methods I'm not equipped to do (EDS/EELs, etc) and many of you sent
such suggestions. Thanks again.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Aug 20 14:02:13 2001



From: Katjaalex-at-aol.com
Date: Mon, 20 Aug 2001 14:56:44 EDT
Subject: Jeol 1200 Problems

Contents Retrieved from Microscopy Listserver Archives
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Hi, we have this problem with the Jeol 1200. It will not start, the main
relay will not hold. If it starts, once in a blue moon, the forepump will or
will not run. We replaced already the motion detector on the pump. If it
runs, the forepump could stop after a few hours and shut down the instrument.
Does anyone have any ideas, water is running well, air is good.
Also I need urgently better schematics, the books are not very helpful. We
are willing to pay for them.
Please give us any ideas. Thank you
Peter Stolzenberg,  215-699-6160    Fax 215-699-5275


From daemon Mon Aug 20 14:07:31 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 20 Aug 2001 14:02:31 -0500
Subject: Re: LM - Something I just don't get

Contents Retrieved from Microscopy Listserver Archives
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Bruce,
In light microscopy, there is not a quantitative relation
between NA and magnification, but as you have observed the numbers
are usually correlated. In theory, resolution in light micrscopy
depends on the NA, not on the magnficiation. The wider angle light
gathered the smaller your diffraction limited spots are comprising
the image. So in theory, one could build a 1x lens with a 1.4 NA and
then just enlarge the image secondarily as needed. But the trouble is
that in practise, I don't believe that optical engineers could make
such a lens, or at least not so that anyone could afford to buy it.
To function in the low power regime it would need to image a large
amount of sample and therefore the front surface of the lens would be
immense (maybe as big as a 35 mm camera lens) and I don't think the
glass can be manufactured that well. So as the mag goes up, the area
sampled on the object goes down and this allows lenses with higher
NAs to be built. Depending on the needs and the cleverness of the
manufacturers, some rather nice lowish mag high NA lenses can be
made. I have a 40x 1.4 NA lens that works delightfully well, and
better than the 100x 1.3 NA lens by the same company.

Hope this helps a tad,
TB
}
}
} Numerical aperture/magnification/image brightness
}
} Why does numerical aperture increase with magnification, rather than
} decrease? In fact, why is there a relationship between magnification and
} numerical aperture at all? If increased numerical aperture means better
} light gathering ability, that implies that higher magnification objectives
} should produce brighter images.
}
} This, to me, is like saying that going from a 50mm camera lens to a 1000mm
} lens is going to give me more light. In the photographic world, increased
} light gathering ability comes only from increased lens diameter and it is
} completely independent of the "magnification" of the lens.
}
} I visited the Nikon N.A. Java tutorial at
} http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
} and this only seemed to reinforce the discrepancy for me.
}
} With an SEM I can imagine a scan over a smaller area (a tighter scan)
} producing an increase in magnification. In a photographic system I can
} envision viewing a smaller ray bundle (smaller solid angle) producing a
} larger magnification. How is it that in light microscopy this is reversed
} and a high magnification objective takes in a larger angle? What is backward
} in my thinking?
}
} Bruce Girrell
} Microline Technology Corp.
} 2397 Traversefield Dr.
} Traverse City, MI 49686
} http://www.microlinetc.com
}
} (231) 935-1585 (Voice)
} (231) 922-5099 (FAX)
} bigirrell-at-microlinetc.com

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Mon Aug 20 14:14:22 2001



From: JHoffpa464-at-aol.com
Date: Mon, 20 Aug 2001 15:09:11 EDT
Subject: chatter

Contents Retrieved from Microscopy Listserver Archives
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i am doing renal bx, have been having trouble today with extreamly fine
chatter. i am using araldite and an LKB nova. i can't tell if it's the
building, or the microtome, or the blocks. the blocks seem the right hardness.
john Hoffpauir


From daemon Mon Aug 20 14:59:04 2001



From: HANSON_JEFFREY_C-at-Lilly.com
Date: Mon, 20 Aug 2001 14:46:51 -0500
Subject: Details for Imaging Workshop on FDA regulation 21 CFR Part 11

Contents Retrieved from Microscopy Listserver Archives
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This message is for those of you who may not have made it to the M&M 2001
last week or missed the pharmaceutical session where we discussed FDA
issues.

Jeff Hanson & Mike Esterman
Scientific Imaging Center
Eli Lilly and Company

************************************************************************
21 CFR Part 11 Imaging Workshop
A workshop for defining "best practices" for
image data management to share with the US FDA
September 24-25, 2001
Indianapolis, IN

Do you know if your image is compliant with FDA standards?
Do you know the FDA's regulations regarding the appropriate handling and
storage of images?

The reality is that these standards are still not defined to any degree of

certainty. That's why Eli Lilly's Scientific Imaging Center is sponsoring

a first-ever Imaging Workshop with companies in a variety of fields. This

2-day workshop will be held on September 24-25, 2001 at the University
Conference Center in Indianapolis, IN.
If you are involved in generating or storing scientific images that could
be included in FDA submissions or in support of a submission, the 21 CFR
Part 11 guideline may affect you. This workshop will attempt to document
a set of best practices for handling and storage of scientific images and
to suggest guidelines to the FDA for applying 21 CFR Part 11 to Scientific

Images.

Scientific Image Center
Eli Lilly and Company


WHO SHOULD ATTEND
This workshop is intended for scientists, QA professionals, and IT
professionals who work with scientific images and imaging within
industries regulated by the FDA. Vendors of instruments and software
supporting their scientific imaging processes are also welcome to
participate.

WORKSHOP OBJECTIVES
The primary objective of this workshop will be to define a set of best
practices for image data management that can be submitted as a set of
recommendations to the FDA. A secondary objective will be to raise
awareness of 21 CFR Part 11 and determine how it will impact scientific
imaging. Finally, a post-workshop working group will be assembled to
follow-up on the conclusions and continue to work through any open issues.

REGISTRATION
You may send an email message to the Scientific Imaging Center at Eli
Lilly and Company for additional information or to find out more about
registration (ImageCtr-at-Lilly.com). Registration needs to be complete by
August 31, 2001.
Please note that space is limited, so we would prefer to have a single
representative from each company.
************************************************************************


From daemon Mon Aug 20 15:11:51 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 20 Aug 2001 15:07:52 -0500
Subject: Re: Choosing an Analytical SEM for Metallurgical Research

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} Date: Mon, 20 Aug 2001 08:53:13 -0700
} To: Ike Oguocha {oguocha-at-yahoo.com}
} From: Mary Mager {mager-at-interchange.ubc.ca}
} Subject: Re: Choosing an Analytical SEM for Metallurgical Research
} Cc: Microscopy
}
Dear Ike,
Any of the four main manufacturers of SEMs: Hitachi, JEOL, Philips (FEI)
and LEO make SEMs suitable for general metallurgical analysis. Likewise, any
of the manufacturers of EDS systems, of which there are too many to name
here, make systems that will suit your purposes. Make a wish list of
capabilities: sample size, stage movement and automation, variable vacuum
(yes or no), kV range, detectors (i.e. BSE type), EDS features and then ask
the Canadian representatives of the SEM manufacturers for their information
on their microscopes and a list of a few Canadian customers that you can
phone or e-mail for information on the durability and service record of each
of the SEMs. To write a grant application you just need a quote from each of
the four, so you know how much money to ask for. Choosing exactly which SEM
and which EDS can come later, after you have the grant.
} At 07:03 PM 8/17/01 +0100, you wrote:
}
} } Hello Netters:
} }
} } In about three weeks time or so, I'll be writing equipment grant
} } appilcations. Our lab needs, as a matter of necessity, an analytical
} } SEM. The one we have now is relatively old (Philips SEM515) and
} } has no EDS facilities. It hinders most of our work since we have
} } to go elsewhere and pay for any work involving EDS. Our attempt
} } last year to get a EDS system grant for it failed. So, we are
} } making a completely new equipment grant this time.
} }
} } I am wondering if there are metallurgical microscopists in the
} } house to give me some insights on which direction to go. What
} } must we be looking for in a good analytical SEM? Who makes what?
} }
} } Many thanks in advance.
} }
} } Ike Oguocha
} } Department of Mechanical Engineering
} } University of Saskatchewan
} } Saskatoon, Canada
} }
} Regards,
} Mary
}
}
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca


From daemon Mon Aug 20 17:35:16 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Aug 2001 15:27:54 -0700
Subject: Re: LM - Something I just don't get

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Let me try to give it a stab--

At 09:28 AM 8/20/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

NA does not increase with magnification. Better objectives have
higher NA as they increase in magnification. The relationship
between NA and magnification revolved around the issue of
resolution (resolving power).

The greater the resolving power is, the smaller the distance
between objects that can be distinguished. Resolving power is:

RP=Lambda/2NA

where Lambda is the wavelength of light. So, to increase RP,
decrease wavelength and/or increase NA. Decreased wavelength
is the same as increasing frequency. White light is a combination
of all colors (many different wavelengths). Removing most the
wavelengths via a filter (IR, for example) will improve RP.
The "frequency" of an electron beam is super high and thus,
has extraordinary RP.

Brightness is mostly due to the amount of glass in a lens.
For high brightness and high NA, use a PlanAPO objective.
Bring lots of money.


} This, to me, is like saying that going from a 50mm camera lens to a 1000mm
} lens is going to give me more light. In the photographic world, increased
} light gathering ability comes only from increased lens diameter and it is
} completely independent of the "magnification" of the lens.

A 50mm/f2 lens will have the same brightness as a 1000mm/f2 lens.
Unfortunately, f-stop is the ratio of the focal length divided by the
diameter of the focusing element. So, a 50mm/f2 lens would have to
have a front lens element which is about 25mm in diameter. The
1000mm/f2 lens would have a front element which is 500mm in
diameter. This huge piece of glass would weigh about five pounds
and the whole lens would cost about $15,000. My Nikkor 600mm/2.8
AI-EDIF weight about thirty pounds and cost $8500. It is definitely
not for point and shoot events.

The alternative to refractive lenses are reflective or Catadioptric
"lenses." These are mirrors, like large telescopes. They have
fixed f-stop figures and are small and light. But the typical f-stop
limit is f8.


} I visited the Nikon N.A. Java tutorial at
} http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
} and this only seemed to reinforce the discrepancy for me.
}
} With an SEM I can imagine a scan over a smaller area (a tighter scan)
} producing an increase in magnification. In a photographic system I can
} envision viewing a smaller ray bundle (smaller solid angle) producing a
} larger magnification. How is it that in light microscopy this is reversed
} and a high magnification objective takes in a larger angle? What is backward
} in my thinking?

A SEM offers huge depth of field (high working distance) at low to
medium magnification. This is due to many factors. One is the
high frequency of the electrons and the extreme collimation of the
electron beam (not a lot of extraneous energy hitting the specimen).
SEMs still suffer from spherical abberations and astigmatism. This is
diminished by stigmator coils. For optical lenses, it is solved pretty
much by lots of money at time of purchase.

gary g.

http://photoweb.net




From daemon Mon Aug 20 18:08:20 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 20 Aug 2001 18:02:54 -0500
Subject: Re: LM - Something I just don't get

Contents Retrieved from Microscopy Listserver Archives
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I agree with Gary Gaugler's description (the entire reply is appended
below) but let me add one minor refinement to his comment :
" Brightness is mostly due to the amount of glass in a lens. For high
brightness and high NA, use a PlanAPO objective. Bring lots of money."

I agree with this for brightfield but for high brightness with UV
(e.g., calcium sensitive fluroochromes like Fura or DAPI), the large
amount of glass in a highly corrected PlanApo works against
brightness. A high NA achromat can sometimes be a better choice if
you don't have a big field of view.

}
} -----------------------------------------------------------------------.
}
}
} Let me try to give it a stab--
}
} At 09:28 AM 8/20/2001, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Mon Aug 20 21:12:24 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 20 Aug 2001 19:05:05 -0700
Subject: Re: Jeol 1200 Problems

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Try the following trick: turn OFF microscope as usual, wait until all
lights is OFF on the panel, than disconnect instrument from main power
source (we do have some huge breaker on the wall), turn ON microscope as
usual.

Good luck, Sergey

At 02:56 PM 8/20/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Aug 20 21:29:51 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Aug 2001 19:47:16 -0700
Subject: Re: TEM of paper & ink

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Any short run publication pre 1950 can be assumed to be printed on letter
press. Duplicate journals would be a good place to look. Anything
printed by the college pre 1950 will fall in that category as well unless it
was running a experimental print shop. A stop by the university print shop
should get you some modern samples of printing and ink. They still probably
do some things that way or know some one that does. It still is hard to beat
for short runs on weird shaped stuff.

You can look at some old books under a LM and see what letter press looks
like and sort though the modern stuff. Letter press hasn't changed enough
in the last 100 years to show up much different under a LM.

To get much of a idea of what the film actually looks like I think you will
have to section the page edgeways.

One advantage of using pre 1950 inks is that there will probably be lead in
them and that should increase the contrast. I think many of the pigments and
dryers are metals so you might get x-rays fluorescing out of them as well
at high voltage.

Since 1950 more and more attention has been paid to safety in the printing
industry. That translates in to less use of heavy metals in inks. So old
inks are likely to image better without enhancement than modern inks. Opaque
blue and green inks would probably be good choices. Cobalt was used in blue
ink until as late as 1970 and I haven't kept up since then.

If you can't find samples I can find some send them to you.

The inks are not a lot different between the two processes. Letter press ink
will work in an offset press and visa versa but they will not work as well
as they will in the press they are designed for. Black printing ink can be
made by grinding carbon black in boiled linseed oil. Form there the
chemistry gets increasingly complicated. Today inks are usualy soy bean oil
based due to health concerns.

Coated papers will have more ink build up than uncoated papers. Book papers
that don't appear to be coated have considerable sizing to keep the ink from
being absorbed in the paper fibers and being seen from the back side. This
paper is also loaded with clay to increase it's opacity.

Gordon
----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Cc: "Tobias Baskin" {BaskinT-at-missouri.edu}
Sent: Sunday, August 19, 2001 1:49 PM


OK.....sounds like I am back to cutting up an old book.

I have found some modern samples of letter press from
Tobias' references. But as you point out, the modern inks
may not be the same as yesteryears'.

I shot a check account number today and was rather
under whelmed. It is not on the surface like the laser print.
I think that the challenge of measuring ink on paper is
not all that easy. Probably not a big surprise to most folks.
But the study of thin films is very useful and translatable to
many other products.

Interesting and useful topic. Perhaps, not seemingly so.

gary g.


At 07:26 PM 8/20/2001, you wrote:

} Any short run publication pre 1950 can be assumed to be printed on letter
} press. Duplicate journals would be a good place to look. Anything
} printed by the college pre 1950 will fall in that category as well unless it
} was running a experimental print shop. A stop by the university print shop
} should get you some modern samples of printing and ink. They still probably
} do some things that way or know some one that does. It still is hard to beat
} for short runs on weird shaped stuff.
}
} You can look at some old books under a LM and see what letter press looks
} like and sort though the modern stuff. Letter press hasn't changed enough
} in the last 100 years to show up much different under a LM.
}
} To get much of a idea of what the film actually looks like I think you will
} have to section the page edgeways.
}
} One advantage of using pre 1950 inks is that there will probably be lead in
} them and that should increase the contrast. I think many of the pigments and
} dryers are metals so you might get x-rays fluorescing out of them as well
} at high voltage.
}
} Since 1950 more and more attention has been paid to safety in the printing
} industry. That translates in to less use of heavy metals in inks. So old
} inks are likely to image better without enhancement than modern inks. Opaque
} blue and green inks would probably be good choices. Cobalt was used in blue
} ink until as late as 1970 and I haven't kept up since then.
}
} If you can't find samples I can find some send them to you.
}
} The inks are not a lot different between the two processes. Letter press ink
} will work in an offset press and visa versa but they will not work as well
} as they will in the press they are designed for. Black printing ink can be
} made by grinding carbon black in boiled linseed oil. Form there the
} chemistry gets increasingly complicated. Today inks are usualy soy bean oil
} based due to health concerns.
}
} Coated papers will have more ink build up than uncoated papers. Book papers
} that don't appear to be coated have considerable sizing to keep the ink from
} being absorbed in the paper fibers and being seen from the back side. This
} paper is also loaded with clay to increase it's opacity.
}
} Gordon
} ----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} Cc: "Tobias Baskin" {BaskinT-at-missouri.edu}
} Sent: Sunday, August 19, 2001 1:49 PM
} Subject: Re: TEM of paper & ink
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Tobias Baskin pointed out the probable difference between
} } Gutenberg printing and modern web printing.
} }
} } I was wondering about that too. Gutenberg. Heard about it
} } but not certain what it actually is. I suspect the magazine is
} } indeed offset web press. But if the the two processes
} } are different, are they both based on ink of the same type?
} } If so, I can't see it in the SEM either SE or BSE. But sure
} } can under LM.
} }
} } I suspect that based on the variety of old books I have,
} } there may or may not be a build up of ink on the paper.
} } Some paper is coated stock whereas others are quite
} } porous. As I think about this, I think the reason I cannot
} } "see" the web printing ink is because it is more "pushed"
} } into the paper. It is akin to being absorbed by the paper.
} } The laser toner is distinctly different and is laying on top
} } of the paper. The laser fuser heats up the toner particles
} } (readily visible as such) and melts and sticks the particles
} } to the paper rather than having the paper absorb them.
} } SEM imaging of the web printing reveals nothing but the
} } texture of the paper fibers. The ink is embedded but
} } is difficult to visualize using SE or BSE.
} }
} } If I knew the age of Gutenberg type of printing, I do have
} } some rather old books around. Some are late 1700's up
} } to early 1900's. A small piece of one could
} } be sacrificed in the name of science!
} }
} } I'm working on thin layers of Au on organic substrates
} } for doing ultrasonic imaging in catheters. I find some
} } similarities between this and the ink question. Quite
} } interesting and useful.
} }
} }
} } Regarding the pix at my web site--
} } JPEGs of same root name are at the site too. These are
} } only about 45KB-80KB each. These may be better to access since
} } some browsers use Quicktime to display TIFF whereas
} } IE for example will directly display JPEG. In this case,
} } just grab them via http://www.gaugler.com/name.jpg
} }
} } For example, http://www.gaugler.com/laserpaper-2.jpg
} } All image files are sized to be 450pixels wide.
} }
} } I also added two files:
} } printedpaper6-bsel.tif
} } printedpaper6-bsel.jpg
} }
} } which are TIFF and JPEG images of printedpaper6-bse
} } after processing with Image Content's Lucis. I'm certainly
} } no paper expert, but it looks like with web printing, the
} } ink is absorbed into the paper's fibers. The lower right
} } appears to be a glob of ink. What is puzzling is that
} } under DIC LM, both laser and web printing have three
} } dimensional aspects. This is not seen in SEM for
} } both types of printing. Perhaps the Gutenberg method
} } will indeed show up.
} }
} } gary g.
} }
} }
} }



From daemon Mon Aug 20 22:56:20 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 21 Aug 2001 07:30:20 -0500
Subject: FW: Resin embedding of porous coal chars for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you are really interested in comparing methods having a shop print up
samples with both presses using the same color that has a metal oxide as a
pigment would probably be the best approach. A small shop could do that in
30 minutes time by washing up the two presses and reinking them and then
cleaning them up again.

Get some samples of the various colors of ink they have an see how they
image first. One or two should have pretty good contrast. If they don't you
can make your own ink by mixing what every powder you want with transparent
base and ink with no pigment. Metal powders don't work very well. They don't
stay dispersed it might be OK for imaging but it sure is hard to get them to
look good to the eye.

If you mix in your own pigment make sure they wash the press as soon as they
are done. Most inks they use today dry very slowly on the press and some
pigments are natural dryers and can cause the ink to dry on the press in an
hour or less. Reds are particularly bad actors in this regard.

Gordon

----- Original Message -----
} From: "Monson, Frederick C." {fmonson-at-wcupa.edu}
To: "'Microscopy Listserver'" {microscopy-at-sparc5.microscopy.com}
Cc: "'Leona Cohen-Gould'" {lcgould-at-med.cornell.edu}
Sent: Monday, August 20, 2001 9:55 AM



If the particles are "small" then the previous suggestion to centrifuge ought
to work well. If the particles, however, have lots of pores, then vacuum
infiltration would be my choice.

Nothing works if the pores are truly sealed, as in pumice, but most difficult
specimens can be infiltrated with vacuum. There is an easy method and a good
method, but easy may be good enough:
Easy:
1 Place specimen in suitable container and the container into a larger
container to take any "mess".
2 Cover specimen with a low viscosity, undiluted resin; if
required weigh down
specimen.
Place into a vacuum chamber. Evacuate briefly, stop evacuation just before
resin's boiling point.
Vent chamber and then re-evacuate.

Note boiling creates bubbles. The method pulls air out of the specimen and the
resin is forced in when venting.

In the "good" method the specimen is evacuated first and the resin is added
while under vacuum. This avoids most bubbles in the resin and takes more air
out of the pores. Apparatus for this is available, but for small scale work I
used a very simply modified vacuum desiccator.

"Good method":
1 As in one above, weigh specimen down.
2 Find a rubber bung that would seal the top valve hole in a
desiccator lid (or
cut a suitable hole in a small plastic desiccator lid). Cut a hole into the
bung that would take the stem of a plastic Pasteur pipette.
3 Find a very large capacity (bellow type) polyethylene Pasteur
pipette. Fill
pipette with "sufficient" resin.
4 Insert stem of pipette through the hole in that bung, have
only a couple of
cm protrude past the smaller end of the bung. Kink and apply a bulldog clip to
the pipette stem above the upper end of the bung.
5 Evacuate the specimen using the side port of the desiccator.
Close the vacuum
valve and remove the bulldog clip. The resin should be pulled into the chamber
and run over the specimen.
6 Leave under vacuum for a couple of minutes and then vent.

Hope this helps.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, August 20, 2001 12:24 PM, Dave Felon
[SMTP:emudp-at-mail.newcastle.edu.au] wrote:
}
} G'day
}
} I have some students who want to examine cross sections of very porous
} coal char in the SEM. Trying to get bubble free blocks is proving difficult
} as the particles tend to float in the resin (so I am told) and it is
difficult
} to
} get good impregnation. Even recoating the ground surface and repolishing
} leaves an unacceptable amount of bubbles in the surface and in the hollow
} particles. Has anyone perfected a technique for similar samples and be
} willing to share it? We usually have no problems with polished blocks, it is
} just this material!
}
} Thanks
}
} Dave
}
}
}
}
} Dave Felon
} EM/X-Ray Unit
} University of Newcastle
} NSW 2308
} AUSTRALIA
} Ph 02 4921 5667
} Fax 02 4921 7019
} emudp-at-mail.newcastle.edu.au


From daemon Tue Aug 21 07:54:51 2001



From: DANIEL EBERHARD :      daniel.eberhard-at-biologie.uni-bielefeld.de
Date: Tue, 21 Aug 2001 14:49:30 +0200
Subject: lacZ and gfp antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I´m searching for two anti-bodies directed against two reporter
gene products
a) beta-galactosidase
b) green fluorescent protein

which can be used for immunomicroscopy, especially for tissues
which are prefixed
with pFA. Any comments or suggestions?

Thank you
Daniel


-----------------------------
* *
* Daniel Eberhard *
* Developmental Biology *
* & Molecular Pathology *
* University Bielefeld *
* Universitätsstr. 25 *
* 32615 Bielefeld *
* Germany *



From daemon Tue Aug 21 08:43:52 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Tue, 21 Aug 2001 09:36:38 -0400
Subject: Vacuum coater

Contents Retrieved from Microscopy Listserver Archives
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Hi TEM'ers

I need some advice on a new vacuum coater for a TEM lab. I presently have a
13 year old Denton 502A system which has performed satisfactorily. Our main
use is for thermal evaporation of Au, C and Al. Denton no longer sells this
model but has a new system, an "Explorer 14". I have also gotten a quote
for an Edwards "Auto 306" which I know little about. Are there other systems
I should be considering? I was considering diffusion systems for
reliability. Are turbo systems reliable, and agressive enough now? I would
appreciate any comments on the performance of these or other systems.
Please respond directly.
Thanks for your time.
Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com




From daemon Tue Aug 21 09:36:38 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 21 Aug 2001 10:28:34 -0400
Subject: RE: LM - Something I just don't get

Contents Retrieved from Microscopy Listserver Archives
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Hi Bruce,
I've done what I think is correct below in red - just in case it's
wrong.

} ----------
} From: Bruce Girrell
} Reply To: bigirrell-at-microlinetc.com
} Sent: Monday, August 20, 2001 12:28 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM - Something I just don't get
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Numerical aperture/magnification/image brightness
}
} Why does numerical aperture increase with magnification, rather than
} decrease? In fact, why is there a relationship between magnification and
} numerical aperture at all?
} } } I don't remember an equation relating N.A. and magnification.
The only one I ever knew was Resolution=(0.61*Wavelength)/index of
refraction * sine alpha.{Don't you just love text} Numerical aperture=index
of refraction * sine alpha! If you consider the meaning of this, you will
readily see that N.A. is dependent on the one had on the optical character
of a material between object and objective lens and alpha is the half angle
of the aperture angle of the objective lens. The latter can be modified by
the designing engineer. All of the above derives from a consideration of
diffraction by the specimen and capacity of a lens as defined by its
geometry.

} If increased numerical aperture means better
} light gathering ability, that implies that higher magnification objectives
} should produce brighter images.
} } } But what is implied if you CAN get a well-illuminated and highly
magnified image from a dark object such as a section of plant stem? A
bright back light, of course. Everything in the optical axis is designed to
take advantage of the design characteristics of the imaging system. The
light is not only bright but focused by the condenser. The specimen is
transparent. Oil is added to increase the index of refraction between
objective and object (then, as a afterthought, between object and
condenser).

} This, to me, is like saying that going from a 50mm camera lens to a 1000mm
} lens is going to give me more light. In the photographic world, increased
} light gathering ability comes only from increased lens diameter
} } } What is your purpose when you change photographic lenses, and why does
the higher power lens have to be of greater diameter? This is the key, I
think. Remember, the first compound microscope? The first guy who bought
one put the objective to his eye and cursed. The compound microscope is
engineered [and so is the camera lens]. Why? Different purpose! Tube
length, lens geometry, working distance, depth of focus, etc. are engineered
to perform a task. Further, in the microscope, magnification is related to
working distance and lens geometry. Light gathering capacity is then
determined by the illuminator and the condenser (i.e., how much of the
available light can be focused on the "field of view". The confocal
microscope was patented in the '60's. It was a brilliant idea without a
proper light source.
} and it is
} completely independent of the "magnification" of the lens.
} } } I think from what I said above, it is clear that N.A. is NOT
dependent on mag., but it IS often described as the capacity of a lens to
gather light. Alpha, in the equation above is really limited by the
geometry of the objective lens, but the intensity of the illumination is
dependent on the geometry (and adjustment) of the condenser as well as the
power of the "light". All of this is necessary, because the object is NOT
a(the) light source [we have to use Kohler logic to fool it], while in the
use of the camera, we depend on the back or "ambient" light or an added
"reflected light" for illumination.

} I visited the Nikon N.A. Java tutorial at
} http://www.microscopyu.com/tutorials/java/objectives/nuaperture/index.html
} and this only seemed to reinforce the discrepancy for me.
}
} With an SEM I can imagine a scan over a smaller area (a tighter scan)
} } } Why don't we do it that way then?
} producing an increase in magnification. In a photographic system I can
} envision viewing a smaller ray bundle (smaller solid angle) producing a
} larger magnification.
} } } Haven't you just described the function of the front lens without
considering the functions of the others behind it?
} How is it that in light microscopy this is reversed
} and a high magnification objective takes in a larger angle?
} } } Again, I think it is all about the geometry of the objective lens and the
intent of the optical engineer.
} What is backward
} in my thinking?
} } } Nothing! Did you ever get a question on an exam that had four story
lines only one of which is relevant? Here, you are trying to understand a
microscope by studying a camera. They are DESIGNED to achieve different
outcomes. Practical question. Have you ever heard of anyone using a camera
lens to achieve higher magnification with a microscope or a microscope
objective to get higher "zoom" with a camera? Neither have I.

} } } [When I was just a freshman in college, I was advised to change my major
from Biology to Mathematics. I was a biology major, I explained, because I
didn't have sufficient confidence in my mathematical acumen to be a
Chemistry major (one "A" in PChem every 4-5 years) which ought to explain
why I wouldn't consider switching to Math. Thank God for the Slayter's (see
below) of this world who have taken on the task of instructing me in spite
of my computational weaknesses.]

There is a GREAT book by one Elizabeth Slayter entitled "Optical
Methods in Biology", Wiley-Interscience, NY, 1970. There is a more recent
edition. I have used her instruction to provide help on questions whose
answers I should remember from physics.

Regards and sorrow for forcing you to suffer through my thinking on the fly,

Fred Monson



} Bruce Girrell
} Microline Technology Corp.
} 2397 Traversefield Dr.
} Traverse City, MI 49686
} http://www.microlinetc.com
}
} (231) 935-1585 (Voice)
} (231) 922-5099 (FAX)
} bigirrell-at-microlinetc.com
}
}
}


From daemon Tue Aug 21 12:28:54 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 21 Aug 2001 11:44:22 -0800
Subject: antibodies on LRW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just to summarize some of the information regarding NA and Resolution and
Magnification:
Resolution and numerical aperture are related by resolution =
wavelength/N.A. objective + N.A. condenser.
Brightness, numerical aperture and magnification are related by
brightness= (N.A.)squared /(magnification)squared.
As is apparent from these equations, in an idealized situation, a larger
numerical aperture at a given magnification will yield better resolution and
greater brightness. Things get a little more complicated in real life as
has been astutely pointed out, however.
Glass has different refractive indexes for different wavelengths of
light. White light is composed of red, green and blue wavelengths, and this
presents a problem when specimens are illuminated with full spectrum light
sources. The red, green and blue components of the image will be refracted
by the lens at slightly angles, and so the focal points for the different
wavelengths end up being slightly different and the result is that objects
appear to be surrounded by a color fringe. This phenomenon, called axial
chromatic aberration, is exasperated at greater angles incident to the lens.
In other words, at high mag, the higher the numerical aperture the more
pronounced the chromatic aberration. It's a concept that is easy to
illustrate but hard to explain. The solution, put simply, is to combine
different types of glass which have equal but opposite differences in
refractive index for light at different wavelengths. This involves more
glass, more smart people with handsome salaries to engineer the glass, and
results in an expensive plan-apochromatic lens which is corrected for
chromatic aberration in the red, green and blue spectra. If the application
only requires limited spectra, as in ultraviolet illumination, chromatic
aberration isn't such a concern, and one can get by with less expensive
lenses of the same numerical aperture, and because of less corrective
optics, may indeed be transmit more light to the eye.
Spherical aberration is a defect of lenses in which the surface forms
part of a sphere. High numerical apertures at high magnification exasperate
spherical aberration, so high quality lenses incorporate corrective optics
to bring areas on the circumference of the field of view into focal register
with the center of the field of view. Again, more glass, more smart people,
more money.
So the long and the short of it are that high mag lenses don't
necessarily have high numerical apertures, however resolving power and
brightness will suffer proportionally. High numerical aperture high mag
lenses may incorporate artifact into the image unless they are engineered
with compensating corrections. Some of these artifacts, which are expensive
to correct, may not be an issue under certain circumstances, and may even
hinder optical performance, so it is important to understand what
corrections one is purchasing, and why.

Karl G.




/****************
Karl Garsha
www.uwm.edu/~keg
****************/
----- Original Message -----
} From: "Bruce Girrell" {bigirrell-at-microlinetc.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 20, 2001 11:28 AM


Hi all

How stable are LRWhite sections? Can I cut slices of LRWhite today
and give them to a student lab to do immunogold labelling next week,
without the slices losing or altering their antigen-antibody affinity
or sensitivity?

I have found epon sections stain better with uranyl acetate
immediately after being cut, as compared to uranyl acetate staining
several days later (Lead staining doesn't seem to be similarly
affected). This suggests there are alterations taking place in the
plastic slices between slicing and some time period afterwards. Does
anyone have any similar anecdotes for LRWhite, specifically in regard
to antibody labels? That is, does labelling efficiency drop off over
time in plastic embedded sections?

look forward to hearing from someone in this regard

Steve
--

___________________________________________________
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

Chairman, Educational Outreach subcommittee
promoting microscopy instruction and increased access to microscopes
Microscopy Society of America
http://www.msa.microscopy.com/



From daemon Tue Aug 21 13:48:08 2001



From: David_R_Stadden-at-armstrong.com
Date: Tue, 21 Aug 2001 14:43:20 -0400
Subject: Diesel Particles?

Contents Retrieved from Microscopy Listserver Archives
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Here's one I've never encountered. A colleague just brought me several
roundish, dark brown to black particles, about a millimeter in diameter, taken
from his car. Although they seem fairly hard, they can be cut with knife,
leaving a waxy smear on the microscope slide. Some have flat sides from where
they adhered to the car surface. All have a wrinkled, somewhat bubbled looking
texture. The exterior appears to be slightly soluble in toluene. When
subjected to a hot wire, a brown, oily substance cooks off, leaving a
charcoal-like core. The question is, could this be an emission product coming
from a large diesel generator that was just put into operation at our site?

The Particle Atlas doesn't depict anything quite this large, so I had some
reservations. Much obliged for any thoughts.




From daemon Tue Aug 21 14:45:25 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Tue, 21 Aug 2001 15:35:44 +0200
Subject: Re: lacZ and gfp antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In our hands and for our application the Abcam rabbit polyclonal to green
fluorescent protein worked pretty good in immuno-em with pFA fixed samples.
You will find informations about this antibody at: http://www.abcam.com



°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
MCDB Dept.
Yale University
P.O. Box 208103
New Haven, CT 06520-8103
U.S.A.

Tel.: 203/432-3473
Fax.: 203/432-6161

e-mail: stefan.geimer-at-yale.edu
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Tue Aug 21 15:22:31 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 21 Aug 2001 16:16:18 -0400
Subject: RE: Choosing an Analytical SEM for Metallurgical Research

Contents Retrieved from Microscopy Listserver Archives
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Hi Ike,
Mary got the SEM info right on since she mirrored our experience
with writing a proposal.
With respect to EDS I have only one recommendation. We all depend
on the big four: Oxford, EDAX, PGT and Thermo-Noran. We are also
considering two or three others: IXRF, 4pi Analysis, Quartz X-Ray, Rontek
and Clemex. These run the gamut from PC-based packages to off-the-shelf
components. We are looking at these, because they are considerably less
expensive than the big four and, because we are trying to understand exactly
what it is we want to do with EDS. Also, do we want WDS in addition or
instead?
There is a plethora of information on the subject of EDS on the net,
but there is nothing that will replace a thoughtful consideration of three
lists: needs, wants and wishes. At every level of a grant proposal, a
clear understanding of how to justify each type of component. Though
perhaps not considered a mechanical engineering area, you might do a quick
scan of a methodology called EBSD (electron backscatter diffraction).

With respect to the SEM, there is the expensive path and the CAMSCAN
(http://www.camscan-usa.com/VEGA%20specification.htm#Title) VEGA Series of
variable pressure systems. Include the array of SEM substages available
from Fullam (http://www.fullam.com/Sem_subs.htm#18025), and you have almost
every piece of info you need. The only extra piece of information you may
have to consider is how much variability in pressure you might NEED to
accomplish the goals set aside for the new system.
Good luck and hope you get it.

Fred Monson

Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
CASI Home Page: http://darwin.wcupa.edu/casi/
Schedule: Navigate from CASI Home Page
Please call before visiting.


} ----------
} From: Ike Oguocha
} Sent: Friday, August 17, 2001 2:03 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Choosing an Analytical SEM for Metallurgical Research
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Netters:
}
} In about three weeks time or so, I'll be writing equipment grant
} appilcations. Our lab needs, as a matter of necessity, an analytical
} SEM. The one we have now is relatively old (Philips SEM515) and
} has no EDS facilities. It hinders most of our work since we have
} to go elsewhere and pay for any work involving EDS. Our attempt
} last year to get a EDS system grant for it failed. So, we are
} making a completely new equipment grant this time.
}
} I am wondering if there are metallurgical microscopists in the
} house to give me some insights on which direction to go. What
} must we be looking for in a good analytical SEM? Who makes what?
}
} Many thanks in advance.
}
} Ike Oguocha
} Department of Mechanical Engineering
} University of Saskatchewan
} Saskatoon, Canada
}
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}
}


From daemon Tue Aug 21 15:40:45 2001



From: Todd Kostman :      kostman-at-vaxa.cis.uwosh.edu
Date: Tue, 21 Aug 2001 15:37:11 -0500
Subject: Knifebreaking

Contents Retrieved from Microscopy Listserver Archives
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Hello all,


I have a question regarding breaking glass knives with the LKB 7800
knife-breaker. When I was trained on the instrument, my advisor threatened
me with instant death if I ever tried to break 8mm thick glass strips rather
than the standard 6.4mm we used. Now I have one of my own and I have a
faculty member who would like to use the thicker glass. Has anyone used 8mm
glass with the 7800 successfully, or was my advisor correct?

Thanks for your help


Todd


--
Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
Department of Biology and Microbiology
University of Wisconsin Oshkosh
800 Algoma Blvd
Oshkosh, Wisconsin 54901
Ph: 920-424-3069
Fax: 920-424-1101
E-mail: kostman-at-uwosh.edu



From daemon Tue Aug 21 19:07:51 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Tue, 21 Aug 2001 18:00:11 -0600 (MDT)
Subject: Re: antibodies on LRW

Contents Retrieved from Microscopy Listserver Archives
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I'd say it probably depends on the antigen - I've had blocks that "went
bad" for a particular antibody within a month or two, but I've also used
grids of LRWhite sections that had been cut weeks/1-2 months in advance.
(This was for a course, so we were already stacking the deck in our favor
by using antibodies that labelled really strongly so the students would
see something when they did their labelling - the sections were cut in
advance for them). The intensoity was somewhat reduced, but that may
have been a function of the course environment...things that normally
work well tend to weird out.

Histology people say the same thing; some samples can be cut in advance
and the sections stored, others are more susceptible to air exposure. If
you can keep the sections cold they may be "happier". Cryosections (for
EM) can apparently be stored for months at 4C in methylcellulose and be
just fine!

Tamara

On Tue, 21 Aug 2001, Steve Barlow wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all
}
} How stable are LRWhite sections? Can I cut slices of LRWhite today
} and give them to a student lab to do immunogold labelling next week,
} without the slices losing or altering their antigen-antibody affinity
} or sensitivity?
}
} I have found epon sections stain better with uranyl acetate
} immediately after being cut, as compared to uranyl acetate staining
} several days later (Lead staining doesn't seem to be similarly
} affected). This suggests there are alterations taking place in the
} plastic slices between slicing and some time period afterwards. Does
} anyone have any similar anecdotes for LRWhite, specifically in regard
} to antibody labels? That is, does labelling efficiency drop off over
} time in plastic embedded sections?
}
} look forward to hearing from someone in this regard
}
} Steve
} --
}
} ___________________________________________________
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} 5500 Campanile Drive
} San Diego CA 92182-4614
} phone: (619) 594-4523
} fax: (619) 594-5676
}
} email: sbarlow-at-sunstroke.sdsu.edu
} http://www.sci.sdsu.edu/emfacility
}
} Chairman, Educational Outreach subcommittee
} promoting microscopy instruction and increased access to microscopes
} Microscopy Society of America
} http://www.msa.microscopy.com/
}
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Tue Aug 21 21:54:35 2001



From: cavinm-at-vsl.cua.edu
Date: Tue, 21 Aug 2001 21:48:10 -0500
Subject: Image Processing Toolkit Question

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I'm using the Image Processing Toolkit for Adobe Photoshop to
calculate areas and perimeters of SEM images and was wondering
if anybody could provide a simplified explaination of convex area
and convex perimeter versus just area and perimeter.

Please, respond offline.

Sincerely,

Cavin Mooers, Research Associate
Vitreous State Laboratory
The Catholic University of America
Hannan Hall
Washington, DC 20064
(202) 319-5346phone
(202) 319-4469fax


From daemon Tue Aug 21 21:58:27 2001



From: Brad Storey :      storey-at-lanl.gov
Date: Tue, 21 Aug 2001 21:55:07 -0500
Subject: Surface Scientist Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Nuclear Materials Science Group (NMT-16) of the Nuclear Materials
Technology (NMT) Division at Los Alamos National Laboratory is
seeking an experienced Surface Scientist interested in the complex
surface chemistry of plutonium and other actinide metals. The
successful candidate will lead the surface science effort in the
Microstructure and Microanalysis team. Working independently as the
subject matter expert, this individual will plan and execute
experiments in support of the Pit Manufacturing, Pit Surveillance and
Certification Programs. They will have for their use two newly
acquired instruments: a JEOL JAMP-7830 field-emission gun Auger
Microprobe (Auger, XPS, cold fracture, heating, orientation imaging
microscopy, gas reaction, AFM/STM); and a Kratos Axis-Ultra imaging
XPS system (XPS, Auger, heating/cooling, gas reaction). These
instruments will be installed in the Plutonium Facility at TA-55 in
the summer of 2002, and enhance the experimental capabilities of a
well-equipped material science laboratory that includes: variable
pressure SEM (EDS, WDS, OIM, CL spectroscopy, hot/cold stage); new 5
spectrometer JEOL 8200 Electron Microprobe; diverse x-ray diffraction
capabilities; hardness testers; several modern optical microscopes
with digital cameras; and a nicely equipped metallography line.

Required Skills:
Prior hands-on experience gathering and interpreting Auger and XPS
data. Experience maintaining state-of-the-art UHV materials
characterization equipment. Practical and theoretical knowledge of
Auger and photoelectron spectroscopy. Recent peer-reviewed
publications that include Auger and XPS as characterization tools.
Working knowledge of quantification procedures relating to these
techniques. Strong organizational skills as evidenced by the
flexibility to work on multiple tasks simultaneously. Excellent
communication skills. Ability to obtain a Q clearance, which normally
requires U.S. citizenship.

Desired Skills:
Experience in preparing radioactive materials for analysis.
Demonstrated ability to handle and work with hazardous chemicals in a
safe manner. Considerable knowledge and experience in any of the
following technical areas: handling and conducting experiments with
actinide metals, alloys and compounds; working in labs containing
radioactive materials; and applying quality assurance requirements to
sampling and analysis activities. Experience in gathering and
interpreting technical information and reporting technical results
both orally and in writing. Active Q clearance and PSAP.

Education Requirement:
Ph.D. degree in Materials Science, Physics, Chemistry or equivalent
combination of relevant education and experience.

Additional Requirements:
This position is subject to the requirements of the Personnel
Security Assurance Program (PSAP). Candidates invited to interview
for this position will be subject to a pre-employment screening
check, medical examination and drug test, and must consent to be in
the program at the time of the interview.

The position described above will be posted at the official LANL
Human Resources Department's web page for job openings. Any
individual interested in this position MUST apply through this web
site to be formally recognized as an applicant. The HR web site also
has guidelines for TSM salaries. Any questions about this opening can
be directed to:
Brad Storey
(505) 667-0458
storey-at-lanl.gov
or
Rollin Lakis
(505) 665-9814
rlakis-at-lanl.gov


***************************************************
Brad Storey, Ph.D.
Team Leader, Microstructure & Microanalysis
NMT-16 - Nuclear Materials Science Group
Nuclear Materials Technology Division
Los Alamos National Lab
PO Box 1663, MS E574
Los Alamos, NM 87545

Tel.: 505-667-0458
FAX: 505-665-7815
pager: 505-996-3129
storey-at-lanl.gov
***************************************************


From daemon Tue Aug 21 22:14:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 21 Aug 2001 20:09:28 -0700
Subject: Re: Follow up to our phone conversation

Contents Retrieved from Microscopy Listserver Archives
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Dear Jim:

I am at the point of deciding whether to dump my
Axioplan system for an Olympus BX-51. I'm not sure
that this is a good idea or is a decision in a positive
direction. Thus, I seek your input on this issue.

As I mentioned before, my current Zeiss system is not working
beyond about 200X. Is there someone in the Sacramento CA
region who can work on my system and find out what is
wrong with it? If some minor tweak is all that is necessary,
I certainly don't need to spend another $40K for a new 'scope.
If I can take the system to someone nearby, that would be
good too.

I have the basic following items:

Axioplan 1 stand
44 72 15 100W lamphouse
2ea 44 53 65 condenser turrets
44 52 48 Ph/DIC turret condenser w/ 46 52 68 1.4 top lens
44 53 51 auto-swingout lens assembly with 0.9NA top lens
44 53 50 manual swingout lens assembly with 0.9NA & 1.4 top lenses

Plan Neofluar 1.25/0.075
Plan Neofluar 20X/0.5 Ph2
Acrostigmat 100X/1.25 Ph3
Plan Neofluar 40X/0.75 Ph2
Plan Neofluar 63X/1.25 oil
Plan Neofluar 20X/0.5 with DIC prism
Plan Neofluar 40X/0.75 with DIC prism
Plan Neofluar 40X/1.30 oil
Plan Neofluar 5X/0.15
Plan Neofluar 10X/0.30
Plan Neofluar 63X/1.25 oil with DIC prism
Plan Neofluar 100X/1.30 oil with DIC prism 44 4 80

44 52 11 swingout low power condenser
44 53 12 thing-a-ma-bob

45 29 21 stress-free trinoc head
43 36 05 analyzer
Polarizer
Ph 1,2,3 inserts
DIC 1,2,3 inserts
2ea 45 31 80 DIC nosepieces

Is there any chance for support and/or parts for this system?
Is it a lost cause? You indicated that these systems were
still supported. How can I get such support?

gary gaugler



At 03:58 PM 7/25/2001, you wrote:

} Dear Dr. Gaugler,

} I want to thank you for taking the time to talk to me yesterday. I appreciate
} your honesty and frankness. We are always glad to hear from our customers,
} especially if the feedback is constructive. Certainly, I was pleased to hear
} your praise for the performance our Microscopes deliver. Of course, I was
} surprised to hear of your negative experience with our support
} team. Therefore,
} I would like to answer your email and phone conversation in two parts:

[snip]



From daemon Tue Aug 21 23:57:32 2001



From: Cheng Huang :      HUANG-at-rsbs.anu.edu.au
Date: Wed, 22 Aug 2001 14:50:40 +1000
Subject: Leit-C

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

We would like to order some conductive carbon cement (Leit-c), but were told that we have to pay more than $100 for special package over the price of the cement because of the chemical contents in the cement. We wonder who has bought Leit-C recently and through which local dealer in Australia.
Thanks for any information.
Cheng Huang

-----------------------------------------------------
Cheng Huang
Australian National University
EM Unit, RSBS
Box 475, ACT 2601
Canberra, Australia
Phone: 61-2- 6125-6553
Fax: 61-2- 6125-3218
http://www.anu.edu.au/EMU/




From daemon Wed Aug 22 01:40:10 2001



From: DrJohnRuss-at-aol.com
Date: Wed, 22 Aug 2001 06:47:20 EDT
Subject: Re: Image Processing Toolkit Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From what I have seen coming out of diesel engines it is soot from unburned
fuel. This is not a scientific observation but the observation of a farming.
It comes from running the fuel too rich and in high load conditions. Taken
to extremes I have seen a tractor blowing a plume of black smoke from 12
miles away on a still morning.

Regulations no longer allow setting like this but you can still see engines
putting out black smoke when starting up from a stop. It is more pronounced
in older trucks. The soot particles can be fairly large.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger
----- Original Message -----
} From: {"David_R_Stadden-at-armstrong.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 21, 2001 1:43 PM



In a message dated 8/21/01 9:58:12 PM,
cavinm-at-vsl.cua.edu-at-sparc5.microscopy.com writes:

} I'm using the Image Processing Toolkit for Adobe Photoshop to
} calculate areas and perimeters of SEM images and was wondering
} if anybody could provide a simplified explaination of convex area
} and convex perimeter versus just area and perimeter.

Thought that was covered in the tutorial on the CD. Anyway, the convex area
and perimeter are measured by constructing a bounding polygon (32 sided in
the tool kit) around the feature. This is sometimes called a "taut string" or
"rubber band" outline since it does not follow any indentations in the
periphery of the object. For a convex shape with no internal holes, the
values are identical to the "regular" perimeter and area.

John Russ


From daemon Wed Aug 22 08:21:18 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 22 Aug 2001 14:13:10 +0100 (GMT Daylight Time)
Subject: Re: Semiconductor Contaminants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can offer a story I heard at a meeting. This is actually
about a factory where cameras were assembled.
Contamination on the lenses was subjected to EDX and the
elements seen (Na?, K?) were consistent with saliva.
Rather than ban talking, the management solved the problem
by use of transparent screens above the equipment.

Dave


On Wed, 15 Aug 2001 07:56:07 -0400 Peter Tomic
{PTomic-at-anadigics.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone out there know of a good source, text or website, that deals
} with the issue of contaminants in a semiconductor manufacturing environment?
} I'm putting together a lecture on the subject but I don't want to generate
} SEM's, optical images etc. if this is available readily. Principally I'm
} interested in human spittle, airborne contaminates such as skin, hair, cloth
} fibers, etc. or anything that may be generated by human interaction with a
} semiconductor device.
}
} Regards,
} Peter Tomic
} Anadigics, Inc.
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Aug 22 08:58:05 2001



From: William Oxberry :      WOxberry-at-downstate.edu
Date: Wed, 22 Aug 2001 09:51:38 -0400
Subject: Re:chatter

Contents Retrieved from Microscopy Listserver Archives
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1) tighten the block in the chuck
2) tighten the knife in its holder
3) check knife angle(I use 4 deg. clearance angle)
4) try at different times of the day- at my old place my lab was next to
two large turbines and early in the day I occasionally had problems with
chatter

good luck



From daemon Wed Aug 22 09:02:03 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Wed, 22 Aug 2001 09:56:21 -0700
Subject: Durst enlarger bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
Does anyone know the proper bulb for a Durst 138S Laborator enlarger?
Is it a giant 110V, 200W opale Atlas bulb? and if so where can I get one?
Durst has not been very helpful.
thanks for the help,
Beth

******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************Ä




From daemon Wed Aug 22 09:02:58 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 22 Aug 2001 09:57:03 -0400
Subject: RE: Knifebreaking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Todd,
I have a 7800B, and an official "LKB 7800 KnifeMaker Spare Parts
Catalog". In all of the spare parts and in all of instructions which we
also still have, there is NO mention of glass with thickness other than
6-7mm. I have always taken that to mean, "NO THICKER GLASS ON PENALTY OF
DEATH!" Since we kept a 1/2" Dupont knife breaker, there was no need to
pester folks about this, I merely kept the 1/2" glass close to the Dupont,
the "DEATH" notice over the LKB and depended on human nature for the rest.
I was correct, no one was ever seen to carry 1/2" glass the 3 feet from the
Dupont to the LKB. I was always easier to reach up and get a strip of the
6mm from the supply.
Also, if 1/2" glass gave better knives than 1/4", none of us would
have kept the LKB's - I think! I have tried the 1/2" from the Dupont on an
ultramicrotome, and the results were spotty. I thought that the Dupont
broke better thin-sectioning knives from 1/4"(6mm) glass.
Get the man(?) an estimate, and don't use the LKB for something for
which it apparently not designed. Watch me get followed by someone who
breaks 1/2" glass all the time with no visible harm to the LKB or the 6mm
knives. Well, I'm old enough to refuse to change and just be called a
"Grumpy Old Man".

Regards,

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
CASI Home Page: http://darwin.wcupa.edu/casi/
Schedule: Navigate from CASI Home Page
Please call before visiting.

} ----------
} From: Todd Kostman
} Sent: Tuesday, August 21, 2001 4:37 PM
} To: Microscopy Listserver
} Subject: Knifebreaking
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello all,
}
}
} I have a question regarding breaking glass knives with the LKB 7800
} knife-breaker. When I was trained on the instrument, my advisor
} threatened
} me with instant death if I ever tried to break 8mm thick glass strips
} rather
} than the standard 6.4mm we used. Now I have one of my own and I have a
} faculty member who would like to use the thicker glass. Has anyone used
} 8mm
} glass with the 7800 successfully, or was my advisor correct?
}
} Thanks for your help
}
}
} Todd
}
}
} --
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} Department of Biology and Microbiology
} University of Wisconsin Oshkosh
} 800 Algoma Blvd
} Oshkosh, Wisconsin 54901
} Ph: 920-424-3069
} Fax: 920-424-1101
} E-mail: kostman-at-uwosh.edu
}
}
}


From daemon Wed Aug 22 09:18:02 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 22 Aug 2001 10:14:33 -0400
Subject: L.R. White Sections

Contents Retrieved from Microscopy Listserver Archives
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To the person wanting to know if you can keep sections on grids for later
labeling: I don't know as I have never tried it. However I have some
blocks that are up to 7 years old that from time to time I resection and
label with the antibody of the day. In the last few months I have been
working with an assortment of Aurion ultra small gold antibodies that can be
easily visualized in the scope after silver enhancement. I have been using
sections from the same blocks with the same antibodies and I can tell you
that using the ultra small gold increases the sensitivity at least ten times
over Aurion 10 nm gold particles.

Getting back to your original question, my gut reaction is that there will
not be any difference between labeling the sections right away or a week
later. Best of luck and let us know what happens with your week old
sections, Tim

Timothy Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 238 JAH
1020 Locust Street
Philadelphia, Pa
19107
215-513-4798 Work
610-613-8170 Cell



From daemon Wed Aug 22 09:33:10 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Wed, 22 Aug 2001 09:28:08 -0500
Subject: FW: Knifebreaking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Wrong, the thickness of the glass does not affect the knife-maker, let alone
ruin the knife maker.

Understand the workings of that instrument:
The weight of the clamping head determines the pressure which is exerted on the
glass by the upper two pressure points. The clamping arm locks the head in
position, but moving the arm further down does not increase the clamping
pressure at all. Clamping pressure could be increased when breaking thicker
glass. This is done by adding a kilo or so by pressing onto the head, while
locking the clamping head's arm into position.

After clamping and scoring (which is not affected by the clamping of the head,
so long as its down) the lower two pressure points are moved up microscopically
when rotating the breaker wheel. If thicker glass does not break at this point,
you could exert a kilo or two of additional pressure by pushing on the locked
clamping head. Although this is very solid and clamped in position, a little
extra pressure can hasten the break.

Those knife makers are so solidly made that the additional one or even two kg
force I suggest would make no difference.

I expect that the majority of those LKB knife makers in labs today are over 25
years old
and they could last another 25 years. Even when used with 8 or 10mm glass.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, August 22, 2001 6:37 AM, Todd Kostman
[SMTP:kostman-at-vaxa.cis.uwosh.edu] wrote:
}
}
} Hello all,
}
}
} I have a question regarding breaking glass knives with the LKB 7800
} knife-breaker. When I was trained on the instrument, my advisor threatened
} me with instant death if I ever tried to break 8mm thick glass strips rather
} than the standard 6.4mm we used. Now I have one of my own and I have a
} faculty member who would like to use the thicker glass. Has anyone used 8mm
} glass with the 7800 successfully, or was my advisor correct?
}
} Thanks for your help
}
}
} Todd
}
}
} --
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} Department of Biology and Microbiology
} University of Wisconsin Oshkosh
} 800 Algoma Blvd
} Oshkosh, Wisconsin 54901
} Ph: 920-424-3069
} Fax: 920-424-1101
} E-mail: kostman-at-uwosh.edu


From daemon Wed Aug 22 10:12:10 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 22 Aug 2001 10:35:11 -0500
Subject: OEM service vs. Insurance Companies

Contents Retrieved from Microscopy Listserver Archives
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Think of chromatic aberration as different wavelength light rays (i.e.,
chromatic) passing through the same point in a lens being focused at
different points. CA is a function of wavelength and refractive index of
the glass.

Think of spherical aberration as same wavelength light rays (i.e.,
monochromatic) passing through different points in a lens being focused at
different points. SA is a function of the spherical surface of lenses.

It is the challenge of lens design to use different curvature lens surfaces,
in conjunction with different refractive index glasses, to bring light waves
associated with a point in the object to a focus in the image plane.
Planapo objectives are used in conjunction with compensating eyepieces to
complete the correction.

Gary Gill

-----Original Message-----
} From: Karl Garsha [mailto:keg-at-csd.uwm.edu]
Sent: Tuesday, August 21, 2001 12:20 PM
To: bigirrell-at-microlinetc.com; Microscopy-at-sparc5.microscopy.com


Just to summarize some of the information regarding NA and Resolution and
Magnification:
Resolution and numerical aperture are related by resolution =
wavelength/N.A. objective + N.A. condenser.
Brightness, numerical aperture and magnification are related by
brightness= (N.A.)squared /(magnification)squared.
As is apparent from these equations, in an idealized situation, a larger
numerical aperture at a given magnification will yield better resolution and
greater brightness. Things get a little more complicated in real life as
has been astutely pointed out, however.
Glass has different refractive indexes for different wavelengths of
light. White light is composed of red, green and blue wavelengths, and this
presents a problem when specimens are illuminated with full spectrum light
sources. The red, green and blue components of the image will be refracted
by the lens at slightly angles, and so the focal points for the different
wavelengths end up being slightly different and the result is that objects
appear to be surrounded by a color fringe. This phenomenon, called axial
chromatic aberration, is exasperated at greater angles incident to the lens.
In other words, at high mag, the higher the numerical aperture the more
pronounced the chromatic aberration. It's a concept that is easy to
illustrate but hard to explain. The solution, put simply, is to combine
different types of glass which have equal but opposite differences in
refractive index for light at different wavelengths. This involves more
glass, more smart people with handsome salaries to engineer the glass, and
results in an expensive plan-apochromatic lens which is corrected for
chromatic aberration in the red, green and blue spectra. If the application
only requires limited spectra, as in ultraviolet illumination, chromatic
aberration isn't such a concern, and one can get by with less expensive
lenses of the same numerical aperture, and because of less corrective
optics, may indeed be transmit more light to the eye.
Spherical aberration is a defect of lenses in which the surface forms
part of a sphere. High numerical apertures at high magnification exasperate
spherical aberration, so high quality lenses incorporate corrective optics
to bring areas on the circumference of the field of view into focal register
with the center of the field of view. Again, more glass, more smart people,
more money.
So the long and the short of it are that high mag lenses don't
necessarily have high numerical apertures, however resolving power and
brightness will suffer proportionally. High numerical aperture high mag
lenses may incorporate artifact into the image unless they are engineered
with compensating corrections. Some of these artifacts, which are expensive
to correct, may not be an issue under certain circumstances, and may even
hinder optical performance, so it is important to understand what
corrections one is purchasing, and why.

Karl G.




/****************
Karl Garsha
www.uwm.edu/~keg
****************/
----- Original Message -----
} From: "Bruce Girrell" {bigirrell-at-microlinetc.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, August 20, 2001 11:28 AM


Listers,

Since our switch last year from OEM service contracts to service managed by
insurance companies for our EM's, life has been, to put it mildly,
interesting. I will be happy to share the details of our experiences with
anyone who is having to make this decision (or is just interested), but I
didn't want to put a lengthy post on the list if it's not relevant to a fair
number of people. Please let me know if you would like to hear our take on
this issue.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Aug 22 10:49:19 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 22 Aug 2001 11:44:21 -0400
Subject: Vacuum coater

Contents Retrieved from Microscopy Listserver Archives
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Dear Russ,

You may want to consider a "Polaron" Evaporator, made by Thermo V.G.
Scientific in the U.K. They have been manufacturing EM sample prep
equipment for many years. Energy Beam Sciences, Inc. is the U.S. agent for
Polaron and we would be happy to help you in any way we can.

Best regards,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
mnesta-at-ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: Gillmeister, Russ [mailto:RGillmeister-at-crt.xerox.com]
Sent: Tuesday, August 21, 2001 9:37 AM
To: 'MSA'


Hi TEM'ers

I need some advice on a new vacuum coater for a TEM lab. I presently have a
13 year old Denton 502A system which has performed satisfactorily. Our main
use is for thermal evaporation of Au, C and Al. Denton no longer sells this
model but has a new system, an "Explorer 14". I have also gotten a quote
for an Edwards "Auto 306" which I know little about. Are there other systems
I should be considering? I was considering diffusion systems for
reliability. Are turbo systems reliable, and agressive enough now? I would
appreciate any comments on the performance of these or other systems.
Please respond directly.
Thanks for your time.
Russ Gillmeister
Microscopy
Xerox Corp.
RGillmeister-at-crt.xerox.com






From daemon Wed Aug 22 11:12:09 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 22 Aug 2001 08:51:10 -0700
Subject: Re: Knifebreaking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I have a question regarding breaking glass knives with the LKB 7800
} knife-breaker. When I was trained on the instrument, my advisor threatened
} me with instant death if I ever tried to break 8mm thick glass strips rather
} than the standard 6.4mm we used. Now I have one of my own and I have a
} faculty member who would like to use the thicker glass. Has anyone used 8mm
} glass with the 7800 successfully, or was my advisor correct?
}
} Todd -

It's possible, but it requires resetting almost everything - which means
that it won't break "standard" glass properly at the new settings. This is
definitely a "majority rule" situation, unless your faculty member outranks
everyone else...

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Aug 22 11:34:36 2001



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Wed, 22 Aug 2001 09:28:10 -0700 (PDT)
Subject: Re: Follow up to our phone conversation

Contents Retrieved from Microscopy Listserver Archives
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Dear Gary,
We have a Zeiss Axioscope that is setup similar to your axioplan.
Over the last five or so years (maybe longer?) it has been well supported
by SERCO technical services located in Livermore CA. If you're interested
you can contact Emile Meylan or John O'Neill at 1-800-483-0508 they are
both very competent. But if you are going to "dump the axioplan" let me
know....

Jon Mulholland
Botstein Lab, L313
Department of Genetics
Stanford University School of Medicine
Stanford CA 94305-5210

650-725-1609

On Tue, 21 Aug 2001, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Jim:
}
} I am at the point of deciding whether to dump my
} Axioplan system for an Olympus BX-51. I'm not sure
} that this is a good idea or is a decision in a positive
} direction. Thus, I seek your input on this issue.
}
} As I mentioned before, my current Zeiss system is not working
} beyond about 200X. Is there someone in the Sacramento CA
} region who can work on my system and find out what is
} wrong with it? If some minor tweak is all that is necessary,
} I certainly don't need to spend another $40K for a new 'scope.
} If I can take the system to someone nearby, that would be
} good too.
}
} I have the basic following items:
}
} Axioplan 1 stand
} 44 72 15 100W lamphouse
} 2ea 44 53 65 condenser turrets
} 44 52 48 Ph/DIC turret condenser w/ 46 52 68 1.4 top lens
} 44 53 51 auto-swingout lens assembly with 0.9NA top lens
} 44 53 50 manual swingout lens assembly with 0.9NA & 1.4 top lenses
}
} Plan Neofluar 1.25/0.075
} Plan Neofluar 20X/0.5 Ph2
} Acrostigmat 100X/1.25 Ph3
} Plan Neofluar 40X/0.75 Ph2
} Plan Neofluar 63X/1.25 oil
} Plan Neofluar 20X/0.5 with DIC prism
} Plan Neofluar 40X/0.75 with DIC prism
} Plan Neofluar 40X/1.30 oil
} Plan Neofluar 5X/0.15
} Plan Neofluar 10X/0.30
} Plan Neofluar 63X/1.25 oil with DIC prism
} Plan Neofluar 100X/1.30 oil with DIC prism 44 4 80
}
} 44 52 11 swingout low power condenser
} 44 53 12 thing-a-ma-bob
}
} 45 29 21 stress-free trinoc head
} 43 36 05 analyzer
} Polarizer
} Ph 1,2,3 inserts
} DIC 1,2,3 inserts
} 2ea 45 31 80 DIC nosepieces
}
} Is there any chance for support and/or parts for this system?
} Is it a lost cause? You indicated that these systems were
} still supported. How can I get such support?
}
} gary gaugler
}
}
}
} At 03:58 PM 7/25/2001, you wrote:
}
} } Dear Dr. Gaugler,
}
} } I want to thank you for taking the time to talk to me yesterday. I appreciate
} } your honesty and frankness. We are always glad to hear from our customers,
} } especially if the feedback is constructive. Certainly, I was pleased to hear
} } your praise for the performance our Microscopes deliver. Of course, I was
} } surprised to hear of your negative experience with our support
} } team. Therefore,
} } I would like to answer your email and phone conversation in two parts:
}
} [snip]
}
}




From daemon Wed Aug 22 13:00:07 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 22 Aug 2001 13:43:02 -0400
Subject: Catalogs and Manuals for LKB 7800 KnifeMaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have copies of the original documentation for the LKB KnifeMaker

"LKB KnifeMaker Spare Parts Catalog" SPC-7800-E21
"LKB 7800B KnifeMaker Operating Instructions" I-7800B-E05
"KnifeMaker Condensed Instructions" I-7800B-E13

Copies can be made available. Responses/requests OFFLINE only please.

Regards,

Fred Monson


Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
Phone: 610-738-0437
Fax: 610-436-3036
CASI Home Page:
email: fmonson-at-wcupa.edu
CASI Home Page: http://darwin.wcupa.edu/casi/
Schedule: Navigate from CASI Home Page
Please call before visiting.


From daemon Wed Aug 22 13:28:26 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Wed, 22 Aug 2001 14:21:58 -0400
Subject: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
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We have an Oxford ISIS EDS system. As it stands it is not set up to
acquire spectral images. We would like to be able to do spectral image
acquisition on this machine. Is there anyone out there who knows how
the system may be modified (macros, interfaces, whatever) to do it? We
do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
helps.

--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Wed Aug 22 14:08:38 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Aug 2001 12:03:16 -0700
Subject: Re: Durst enlarger bulb

Contents Retrieved from Microscopy Listserver Archives
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Try www.bulbman.com or www.calumet.com

If those fail, call me at 916.791.8191 or e-mail
and I can probably find one locally.

gary


At 09:56 AM 8/22/2001, you wrote:
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From daemon Wed Aug 22 17:51:51 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 22 Aug 2001 18:51:52 -0400
Subject: Re: OEM service vs. Insurance Companies

Contents Retrieved from Microscopy Listserver Archives
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Randy,
I, for one, would be most interested in your experiences and possibly
comparison with your OEM experiences. As a third party service company
I would find the feedback helpful in tweaking my own contracts to better
serve the users. All three modes have pluses and minuses for a number
of valid reasons. How various customers view those +'s, -'s and reasons
for them is helpful to a lot of us on both sides of the fence.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA 17314

Tindall, Randy D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
}
} Since our switch last year from OEM service contracts to service managed by
} insurance companies for our EM's, life has been, to put it mildly,
} interesting. I will be happy to share the details of our experiences with
} anyone who is having to make this decision (or is just interested), but I
} didn't want to put a lengthy post on the list if it's not relevant to a fair
} number of people. Please let me know if you would like to hear our take on
} this issue.
}
} Cheers,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
}
}



From daemon Wed Aug 22 17:59:21 2001



From: benedict-at-email.arizona.edu
Date: Wed, 22 Aug 2001 15:54:30 -0700
Subject: LOOKING FOR A POSTDOC/RESEARCH JOB IN MATERIALS SCI. & ENG.

Contents Retrieved from Microscopy Listserver Archives
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If you have a vacant position for a Ph.D holder in Materials Science and
Engineering (Electron microscopy/Materials characterization) that needs
to be filled immediately, please contact me at benedict-at-u.arizona.edu.

Thanks

Benedict Johnson





From daemon Wed Aug 22 18:01:54 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 23 Aug 2001 08:59:37 +1000
Subject: RE: Leit-C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We supply Leit-C in Oz, though I have not yet listed the material in our
online. I guess its true for many suppliers: however large our online catalogue
is we have ready access to two or three times as many items.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, August 22, 2001 2:51 PM, Cheng Huang [SMTP:HUANG-at-rsbs.anu.edu.au]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} We would like to order some conductive carbon cement (Leit-c), but were told
} that we have to pay more than $100 for special package over the price of the
} cement because of the chemical contents in the cement. We wonder who has
} bought Leit-C recently and through which local dealer in Australia.
} Thanks for any information.
} Cheng Huang
}
} -----------------------------------------------------
} Cheng Huang
} Australian National University
} EM Unit, RSBS
} Box 475, ACT 2601
} Canberra, Australia
} Phone: 61-2- 6125-6553
} Fax: 61-2- 6125-3218
} http://www.anu.edu.au/EMU/
}



From daemon Wed Aug 22 20:30:37 2001



From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Wed, 22 Aug 2001 21:23:20 -0400 (EDT)
Subject: Re: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alwyn:

By spectral images, do you mean xray mapping? A "key" disk for the ISIS
software program is required to activate the xray mapping facility. Of,
course this will cost a bit of money to purchase from Oxford. When the
ISIS program is loaded initially, all the software required to use the
program is there, but activating the unpaid for parts of the program is
the thing.

Fred


On Wed, 22 Aug 2001, Alwyn Eades wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} We have an Oxford ISIS EDS system. As it stands it is not set up to
} acquire spectral images. We would like to be able to do spectral image
} acquisition on this machine. Is there anyone out there who knows how
} the system may be modified (macros, interfaces, whatever) to do it? We
} do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
} helps.
}
} --
} ..........
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvania 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
}
}





From daemon Wed Aug 22 22:08:08 2001



From: Jim Mabon :      mabon-at-uiuc.edu
Date: Wed, 22 Aug 2001 23:22:27 -0500
Subject: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
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I would be interested.
I have never had a "good" experience with any claim from any insurance
company.

The money is the only thing that matters with them.

Regards,

Earl Weltmer

Claimer (not DISclaimer) : The above opinion are my own and are necessarily
the opinion of my company. They are based upon actual experiences with
insurance comapnies over the years from health insurance (Blue Cross), to
auto (Farmer's), to moving Companies (United Van lines, FEDEX).

----- Original Message -----
} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 22, 2001 8:35 AM


Hi Alwyn,

This is something I thought about a while back. Unfortunately, I didn't
come up with any ideal solutions. You can define an 2-D array of points
using the AUTO module and an appropriately short value of live time. This
will save a full spectrum for each point on disk in separate sequentially
numbered files (painfully slow and get a larger disk). I don't remember
what the limits on the dimensions of the array are. The big problem is then
how to analyze the data. Within the ISIS software the only thing I can think
of is to batch process for peak integrals. Ray Twesten has written a short
utility which can be used to batch convert the ISIS format spectra to
1-column ASCII format. From here you could write your own processing
routines
or perhaps use digital micrograph (or a little of both). I'm sure many of us
would be intested if you find a workable solution?

P.S. I just remembered Oxford also had a little known programming API
product,
which could be used to control and read from the ISIS hardware from your own
application. I forget what they called it. I suspect this would be a major
undertaking to work with.

Jim Mabon

-----Original Message-----
} From: Alwyn Eades [mailto:jae5-at-lehigh.edu]
Sent: Wednesday, August 22, 2001 1:22 PM
To: EMNET



We have an Oxford ISIS EDS system. As it stands it is not set up to
acquire spectral images. We would like to be able to do spectral image
acquisition on this machine. Is there anyone out there who knows how
the system may be modified (macros, interfaces, whatever) to do it? We
do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
helps.

--
.........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu



From daemon Thu Aug 23 02:15:41 2001



From: Alan Stone :      as-at-astonmet.com
Date: Thu, 23 Aug 2001 06:27:16 -0500
Subject: Re: SOLICITING FOR YOUR ASSISTANCE

Contents Retrieved from Microscopy Listserver Archives
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I beg to differ and stand by my previous contribution, which is appended last.
Caroline talks about changing settings. Well, the settings adjust the lateral
position of the score mark and how far forward the score is to extend. These
adjustments need to be changed when cutting strips other then squares (25.4mm),
or for non 45 degree knives. Drawing a felt tip marker across the correct
settings (different colours for different settings) makes it easy to find the
correct settings again. Anyway, those settings have nothing to do with the
thickness of the glass. Similarly, the clamping pressure is gravity (weight of
clamping head) and the head will exert the same pressure regardless of the
glass thickness. Dto the score pressure.

Fred is concerned about half inch glass, but the original question concerned
8mm glass. I have no hesitation to use (and have) 8mm glass with the LKB
knifemaker. The 1/4 inch (6.4mm) thickness glass has been a convenient standard
material for EM, but the design is not specific to that thickness. I expect
that 10mm glass would also be no problem with the LKB, half inch (12.6mm) would
require still greater pressure, and perhaps there is a limit, but I cannot see
that reasonably any harm could be done to those very solidly build part of the
knifemaker.

Incidentally, years ago LKB was the sole agent for "LKB" (actually Alkar)
glass. That glass is made up to 10mm thick and I cannot imagine that LKB
marketed that glass for use with another knifemaker.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com


-----Original Message-----
} From: Caroline Schooley [SMTP:schooley-at-mcn.org]
Sent: Thursday, August 23, 2001 1:51 AM
To: Todd Kostman
Cc: Microscopy-at-sparc5.microscopy.com



Dear Earl and all else,

You may think this sounds so extreme that it is beyond belief. Sad to say
that not everyone has sound judgement and have fallen for this ruse. At
worst, the victims are eventually duped to travel to Nigeria and held for
ransom. Some have been killed.

No substitute for earning a living the old fashioned way.

Al Stone




At 07:24 AM 8/18/2001 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Thu Aug 23 07:42:08 2001



From: pmoore-at-wfubmc.edu (Paula Moore)
Date: Thu, 23 Aug 2001 08:31:16 -0400
Subject: Re: chatter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} 1) tighten the block in the chuck
} 2) tighten the knife in its holder
} 3) check knife angle(I use 4 deg. clearance angle)
} 4) try at different times of the day- at my old place my lab was next to
} two large turbines and early in the day I occasionally had problems with
} chatter
}
}

Here are a few more suggestions:

1) Use distilled water in your boat as opposed to de-ionized.
2) If possible re-trim your block. Even though you think things look good,
sometimes just cleaning up the edges with a new razor blade makes things
better.
3) As much as you can, keep your block chuck and knife stage centered on
zero. It seems for me if I swing the knife to the left or right too far,
chatter is a given.
4) Try another knife. I've had diamonds re-sharpened and I swear they came
back duller than they started out. Also something could have happened to the
mount and the diamond may be loose.
5) Pray to the Microscopy gods. :-)

Hope this helps,

Paula Moore
Wake Forest Univ. Medical Center
Pathology/EM Lab




From daemon Thu Aug 23 08:25:38 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 23 Aug 2001 09:17:34 -0700
Subject: durst bulb

Contents Retrieved from Microscopy Listserver Archives
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Problem solved! thanks to Bill Sharp!
As always the Microscopy list is the best!
thanks for all the help,
Beth

******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************cr




From daemon Thu Aug 23 09:31:07 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 23 Aug 2001 09:23:15 -0500
Subject: OEM service vs. Insurance Companies---Long message

Contents Retrieved from Microscopy Listserver Archives
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As promised, here is a rundown on our OEM vs. insurance experiences. I
decided to put this on the list based on the very large number of replies I
received, some asking specifically that this be posted. As a result, I've
removed all names, since I don't have a clue as to legal ramifications of
being more specific. Sorry to be so wimpy, but in these cases I believe in
CYA.

The following obviously reflects our experiences alone, but based upon what
I have heard from a few others, I don't believe that our situation is
unique, by any means.

Our lab, like many others, has been under increasing pressure to cut costs
for the last two to three years, and since service contracts represent a
significant proportion of our budget, they logically became a cost-cutting
target immediately. It was beginning to appear that we were going to lose
the contract on one of our TEM's to save money, especially since it wasn't
heavily used. About this time one insurance company (IC, for short) began a
series of high-powered presentations to the University purchasing people and
administrators, promising significant cost reduction, with no reduction in
services, choice of service providers, elimination of paperwork, etc.
Facilities like ours were contacted by University purchasing staff in order
to set up individual meetings to assess our needs and get estimates on the
IC's costs versus OEM charges.

After attending two open seminars and having two meetings with the insurance
people and purchasing staff in our facility, the decision was made, with my
agreement, to give the IC a shot at managing our service. The thinking was
that we would try it for a year. The IC said we could back out at any time
with no penalty, they would pro-rate our contracts to adjust for the
differing ending dates for our OEM contracts, and, they said, there should
be no "re-certification fee" if we decided to go back to OEM contracts,
since we intended to use only the OEMs' engineers anyway. They would take
care of the paperwork notifying the OEMs about the new arrangement, and all
billing would be handled between the IC and the service providers. They
would guarantee no increase in contract costs for, I believe, three years.

The very first service call we made to the manufacturer on one of our TEMs
resulted in our being told that they couldn't send an engineer, because we
weren't on service contract any longer. When I explained that they were
supposed to have been notified that we were with the IC, I was told that we
would have to provide a University P.O. before anyone would be sent.
Apparently, there was a substantial outstanding bill from this IC for
service provided to a university in another state, and until it was paid no
service would be provided on our contract. The IC's on-campus rep, our
purchasing director, and the director of the university core facilities were
notified by me immediately. Apparently some high level phone calling went
on, because before the day was out, the service provider had called back
saying the outstanding bill would be paid and an engineer was on the way.
(Additionally, our purchasing director assured us that we could get a P.O.
if necessary. We have terrific support here from purchasing and they have
been invaluable on several occasions.)

The engineer was sent out, the scope was back on line. BUT, it turns out
that the promised payment was never made and we were back to square one the
next time we needed service on that machine. In fact, it was worse, because
the OEM was never paid for the service on our scope either.

Our other scopes are from a different manufacturer and initially service was
pretty much the same as before (although they made it clear they weren't
happy about the switch). Response time was a bit slower, and there was more
stinginess with replacement parts, but we had no real complaints.

Then, the IC declared bankruptcy. Their campus reps disappeared, their web
site went down, and they effectively vanished. We had already used part of
the year's contract, but were only able to recover about $700 on the unused
portion. Some labs lost thousands, I've been told. The service provider
was never paid, and lost a substantial amount of money. (I'm told that this
IC has reorganized under a different name and is soliciting new business,
especially from universities. Ask lots of questions if a new company comes
around. Be especially careful of large numbers of enthusiastic people in
nice suits giving slick multi-media presentations!)

We began discussing returning to our old OEM contracts and I was asked to
get prices. The first thing I was told by one service provider was that we
would have to have our instrument re-certified, which would cost about
$1500, even though nobody had touched that scope but the OEM engineers.
Needless to say, that rankled a bit, so we checked into another insurance
company that had been recommended by several other labs. We were
immediately impressed by the fact that they presented what seemed to be a
more realistic picture of what they could do for us. They also offered
significant savings (10-15%) over the old contracts, so we once again
decided to give it a try.

This time, the first call we made had the same result as before, i.e., the
provider wanted a P.O. before an engineer would come out. When I asked why,
they said that even though they had no problems with the new IC, they
weren't going to take a chance on losing a ton of money again. If the
university would sign a waiver accepting responsibility for paying any
amounts in default, we could get service again. Fine, I said. Several
days later the form was faxed to us and I forwarded it to our purchasing
director, who responded that he had passed it along for review to the people
who needed to sign off on it. That was last month sometime, and I have
heard nothing yet. So far we have been waiting to get a preventive
maintenance visit on this scope for about two months and nothing's in sight,
yet.

On our other scopes, we have again been able to get service, but now it
seems that we are way, way down on the priority list. OEMs will tell you
that they are obligated to service their contract customers first, and in
our experience they certainly do. The service management (insurance)
companies will tell you that this is not the case, that it's first-come,
first-served. That has not been our experience. On one TEM we have been
waiting weeks for a PM. It was scheduled twice and an engineer was here,
but was pulled away on "emergency" calls, which I read as calls from
contract holders. The PM has not yet been done. (Just minutes ago we
received a call from our service engineer saying he's been pre-empted again
and now he has NO idea when he can get here.) Our SEM has been serviced
under the new arrangement in a relatively timely manner.

Under the OEM contracts, service was provided "yesterday" and parts were
replaced if they were even suspected to be bad. The working relationship
was wonderful, and the service was stellar. We were called and reminded of
PMs before they were due and we received periodic phone calls just to check
on the status of our equipment. Under the new contracts, service is
provided (if we can get it) whenever they get around to it, and engineers
can be interrupted by calls to respond to contract holders. Parts are
changed less frequently and are all billed. We schedule the PMs (no big
deal). The relationship is much more tense than it used to be.

We could probably increase the level of service by reminding the service
people that when it's time to replace our scopes, service history will have
a MAJOR bearing on whose machines we buy. However, I'm extremely reluctant
to turn a previously very pleasant relationship into a confrontational,
grudging one (although it seems to be headed that way, anyway). In
addition, I can understand why some of these things are happening. If I put
myself in the shoes of the OEM service managers, I'm not sure what I would
do differently. On the other hand, I also have a tiny, nagging feeling that
part of these problems are deliberately designed to pressure us back into
our old contracts. That possibility (and I don't know it to be true) annoys
me intensely, but I still haven't played the sales managers against service
managers card. My sense is that OEMs make big bucks on service contracts
and they take it VERY personally when we "fire them" as one service manager
phrased it.

I would like to emphasize very strongly that when field service engineers
come out, they are uniformly excellent. They do everything they are allowed
to do to provide top-notch care for the instruments. The problems
originate higher up.

In summary, we have found that service management companies do indeed save
money on comparable contracts, but we have had a miserable time getting
service at all when using them. This may not be typical---I don't know.
Our level of service has declined greatly in terms of promptness, attitude,
and parts supply. The attitude part was certainly not worth the extra
several thousand dollars the OEMs wanted, but the promptness and parts
issues probably were. Most disturbing was finding out that we could be
denied service because of a problem originating at another university in
another state that had absolutely nothing to do with us.

We will continue for now with our new IC's to see if the problems correct
themselves, especially since the problems didn't arise with this company.
In addition, one of the OEM service managers called and suggested we might
try a custom contract in which we could work with them to design our own
service schedule. He said we could be informally trained during service
visits to perform many of the maintenance chores on the scope and could
retain a contract that would basically cover emergencies, at a substantial
cost savings. We are looking into this now, because it seems to have lots
of nice possibilities. (We already do filament/gun exchanges and cleanings,
obviously, but I've never personally done a mechanical column alignment or
routine maintenance requiring major disassembly.)

My best advice: if you are currently with an OEM, stay there. If you are
losing your local in-house service wizard and need to decide on contracts,
go with the OEMs. Fight like hell to resist pressure to switch.

If you are with an insurance company and are happy with them, stay there,
and I wish you continued good luck. Please tell me your secret.

My suggestion to OEM service providers: offer some in-house maintenance
training courses, better maintenance manuals and schematics, and encourage
customers to do more maintenance tasks (with appropriate provisos for
covering dumb mistakes). Lower your contract prices so we're not so
pressured to drop them. The glory days are over, folks. Time to compete.

As for me, I have no financial interest in any of these folks. I just want
our scopes to work. Feel free to contact me with any questions.



Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Aug 23 09:38:25 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 23 Aug 2001 10:33:21 -0400
Subject: RE: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
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This is correct, the keydisk is all that is required for
the software to be enabled. However, there may be hardware
required, too, at least a cable interface (RS232?) to the
microscope. I believe the ISIS Autobeam likes to select
its own scan rates. You may need to speak with Oxford re:
compatibility with your particular microscope.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
[SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} Alwyn:
}
} By spectral images, do you mean xray mapping? A "key"
disk
} for the ISIS
} software program is required to activate the xray mapping
} facility. Of,
} course this will cost a bit of money to purchase from
} Oxford. When the
} ISIS program is loaded initially, all the software
} required to use the
} program is there, but activating the unpaid for parts of
} the program is
} the thing.
}
} Fred
}
}
} On Wed, 22 Aug 2001, Alwyn Eades wrote:
}
} }
} }
---------------------------------------------------------
} } ---------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} }
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} } ml
} }
} }
---------------------------------------------------------
} } --------------.
} }
} }
} }
} } We have an Oxford ISIS EDS system. As it stands it is
} } not set up to
} } acquire spectral images. We would like to be able to
do
} } spectral image
} } acquisition on this machine. Is there anyone out
there
} } who knows how
} } the system may be modified (macros, interfaces,
} } whatever) to do it? We
} } do have Digital Micrograph (on a Mac whereas ISIS is on
} } a PC), if that
} } helps.
} }
} } --
} } ..........
} } Alwyn Eades
} } Department of Materials Science and Engineering
} } Lehigh University
} } 5 East Packer Avenue
} } Bethlehem
} } Pennsylvania 18015-3195
} } Phone 610 758 4231
} } Fax 610 758 4244
} } jae5-at-lehigh.edu
} }
} }
}
}



From daemon Thu Aug 23 11:37:24 2001



From: Mike M. :      mike-at-scopemart.com
Date: Thu, 23 Aug 2001 16:28:24 -0700
Subject: EDS/EDX compatibility question

Contents Retrieved from Microscopy Listserver Archives
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We are looking to acquire a Link AN10000 EDS system for a Hitachi H-600 TEM.
Currently this EDS system is on a H-7000 STEM. My questions is: will an EDS
system that fits the H-7000 STEM, also fit the H-600 microscope?

Also, please share your experiences with the Link AN10000 EDS system - its
reliability, support, etc.

Thank you for your help,


Mike


From daemon Thu Aug 23 11:40:02 2001



From: DrJohnRuss-at-aol.com
Date: Thu, 23 Aug 2001 12:34:37 EDT
Subject: Ann: Image Processing and Measurement Workshop

Contents Retrieved from Microscopy Listserver Archives
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The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ through the North Carolina State University Department
of Continuing and Professional Education is now in its 19th year. The course
will be presented October 30 - November 1, in Raleigh, NC. This course has
generated highly favorable reviews from the thousands of previous students.
The primary focus is on images from various types of microscopy, with
practical guidance in correcting imaging defects, enhancing the images for
presentation and measurement, and performing stereological meaningful
measurements on them. Textbooks and computer software are provided to
attendees. Lab sessions with an opportunity to bring your own images makes
this course immediately useful and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.AOL.com/IPCourse/


From daemon Thu Aug 23 12:39:23 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 23 Aug 2001 12:32:20 -0500
Subject: RE: Spectral Imaging with ISIS

Contents Retrieved from Microscopy Listserver Archives
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I am pretty sure that Alwyn is not talking about x-ray mapping. If that was
all he wanted, I suppose the key disk is all that would be needed if he
already has the other imaging applications and hardware installed.

Someone suggested using Auto to scan an image for multiple spectra. Indeed,
the software and hardware should allow it. The spectra might then be
exported and processed by some other package to perform the type of
component analysis that has been described at MSA over the last few years.
My current ISIS x-ray application (version 3.32) has the option of storing
the x-ray data in single column MSA format. Previous versions placed
multiple channels on a single line.

One hitch would be the limitation on the number of spectra per job. I think
there is a limit of 1000 or so built into the database used for managing
the data. I suppose it could be changed to another number, but we bumped
into it some time back.

That would mean you could do a 32x32 raster of points saving a spectrum at
each. A four second acquisition per point would mean a bit more than an
hour for acquisition. That would not be too bad, but the spatial resolution
would probably be too low to be very useful. But the data should readily
fit onto hard drives with capacities of a few gigabytes.

I would be interested in seeing how the data processing is handled. If
someone makes it work, I would be very interested in hearing the details.

Warren

At 10:33 AM 8/23/2001 -0400, you wrote:
} -----------------------------------------------------------------------.
} This is correct, the keydisk is all that is required for
} the software to be enabled. However, there may be hardware
} required, too, at least a cable interface (RS232?) to the
} microscope. I believe the ISIS Autobeam likes to select
} its own scan rates. You may need to speak with Oxford re:
} compatibility with your particular microscope.
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} (305)284-4736
} mlynn-at-miami.edu
}
}
} On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
} [SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
} }
} } Alwyn:
} }
} } By spectral images, do you mean xray mapping? A "key" disk for the ISIS
} } software program is required to activate the xray mapping facility. Of,
} } course this will cost a bit of money to purchase from Oxford. When the
} } ISIS program is loaded initially, all the software required to use the
} } program is there, but activating the unpaid for parts of the program is
} } the thing.
} }
} } Fred
} }
} } On Wed, 22 Aug 2001, Alwyn Eades wrote:
} } } We have an Oxford ISIS EDS system. As it stands it is not set up to
} } } acquire spectral images. We would like to be able to do spectral image
} } } acquisition on this machine. Is there anyone out there who knows how
} } } the system may be modified (macros, interfaces, whatever) to do it? We
} } } do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
} } } helps.
} } } ..........
} } } Alwyn Eades
} } } Department of Materials Science and Engineering
} } } Lehigh University
} } } 5 East Packer Avenue
} } } Bethlehem
} } } Pennsylvania 18015-3195
} } } Phone 610 758 4231
} } } Fax 610 758 4244
} } } jae5-at-lehigh.edu



From daemon Thu Aug 23 13:27:13 2001



From: Macatangay, Peggy J., Celanese/US :      PJMckarns-at-Celanese.com
Date: Thu, 23 Aug 2001 13:13:47 -0500
Subject: SEM-EDS: Help with choosing a new system

Contents Retrieved from Microscopy Listserver Archives
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I am investigating EDS systems to integrate with a JEOL-6100 SEM. I'm
leaning towards PGT's Spirit system. Other considerations are Oxford's INCA
and Thermo Noran's Vantage. Does anyone have particularly positive or
negative experience with PGT? I don't often hear much about that company.
Any comments about the other possibilities would be appreciated, as well.

Thanks!

-Peggy

Peggy McKarns Macatangay, PhD
Project Analyst 2
Celanese Corpus Christi Technical Center



From daemon Thu Aug 23 13:49:13 2001



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Thu, 23 Aug 2001 13:39:52 -0500
Subject: ELMDAS COMPANY

Contents Retrieved from Microscopy Listserver Archives
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Has anyone seen or heard from John Best at the ELMDAS Company?
They/He manufacture the "DIGISEM" SEM Digital Imaging System. I
along with many other people(customers) have left many messages(voice
& email) to no avail. Any help would be greatly appreciated. Please
reply directly to me. Thanks in advance.

Gary M. Easton
Scanners Corporation
{mailto:gary.easton-at-scannerscorp.com} gary.easton-at-scannerscorp.com


From daemon Thu Aug 23 13:49:22 2001



From: john_bruss-at-bose.com ()
Date: Thu, 23 Aug 2001 13:45:37 -0500
Subject: Ask-A-Microscopist: how to suspend a spider in a clear cube

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (john_bruss-at-bose.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 22, 2001 at 17:00:52
---------------------------------------------------------------------------

Email: john_bruss-at-bose.com
Name: John Bruss

Organization: Bose Corp.

Education: Graduate College

Location: San Diego, CA, USA

Question: What materials and process would an amateur use to suspend
a spider in a clear cube for unmagnified artistic and historic use?
Where are those materials available in small quantities?

---------------------------------------------------------------------------


From daemon Thu Aug 23 13:57:13 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 23 Aug 2001 13:52:40 -0500
Subject: Microwave workshop-BIO

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi all,
This post is directed primarily to those doing biological prep in the midwest. I am sure that a number of you were as impressed with the recent advances in microwave specimen prep presented at M&M2001 as I was. I have threatened in the past to have a workshop at Purdue so we could get up to speed on these new techniques.

Well the time has come.....or at least will come if there is sufficient interest. The proposed hands-on workshop, conducted by Rick Giberson of Ted Pella, Inc., would be 2-2 1/2 days in length. Exact cost would not be known until we have an idea of numbers of participants. The dates for the workshop will be set after determining interest but hopefully will be within the next few month.

Available would be two microwaves, 2 TEM's and 1 SEM to check samples, 5 microtomes, misc. other equipment, etc so that all attending would be able to process and check samples.

Purdue is located in West lafayette, Indiana which is ~ 1hr. north of Indianapolis or 2hr SE of Chicago by car. It is serviced by Northwest airlines via the Lafayette/Purdue airport or via the airport in Indianapolis.

Please respond immediately if you are seriously interested in attending a workshop. I will be in touch with more details to those responding as soon as we determine whether the workshop will be held.

Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



From daemon Thu Aug 23 14:18:50 2001



From: Chen Chen :      cche1-at-mail.jhmi.edu
Date: Thu, 23 Aug 2001 15:14:32 -0400 (EDT)
Subject: uranyl formate

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for dist-Microscopy; Thu, 23 Aug 2001 14:17:14 -0500 (CDT)
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for {microscopy-at-sparc5.microscopy.com} ; Thu, 23 Aug 2001 15:10:49 -0400 (EDT)


Hi, everyone,
Does anyone have the recipe and protocol on how to make uranyl formate
solution for negative staining?
And do you have any idea about whhich is better, uranyl aceate or formate?
Thanks a lot.

Chen Chen

Dept of Biol. Chem.
the Johns Hopkins Univ.
School of Medicine



From daemon Thu Aug 23 15:16:31 2001



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 23 Aug 2001 20:55:19 +0100
Subject: Nikon DIC

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Well said.

Thank You,

Earl

----- Original Message -----
} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 23, 2001 7:23 AM


A lab I am working in for a while has a new Nikon Eclipse inverted
and when I went to use it, I could not get the DIC to work. The rep
came by today and fixed it by turning over the slider holding what I
believe is the initial polarizer (the one above the body that
presumably is the first polarizer in the transmitted light path). I
have not seen a real detailed description of the Nikon DIC so I do
not understand what each element is and why this should have had so
great an effect. I thought the rotatable element below the condenser
was a rotatable polarizer, so I thought at some point I would have
gotten extinction even if the orientation of the first was changed.
Thanks- Dave


From daemon Thu Aug 23 16:43:38 2001



From: mcs4-at-dana.ucc.nau.edu ()
Date: Thu, 23 Aug 2001 16:39:24 -0500
Subject: Ask-A-Microscopist:stain for b eta glucans mainly callose

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mcs4-at-dana.ucc.nau.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
August 23, 2001 at 11:04:58
---------------------------------------------------------------------------

Email: mcs4-at-dana.ucc.nau.edu
Name: Matthew Salanga

Organization: Northern Arizona University

Education: Graduate College

Location: Flagstaff, Arizona

Question: I have been doing TEM work on plant cells for my thesis. I
was wondering if anyone might have a suggestion on a stain which
would be specific for
beta glucans mainly callose. Literature suggests that normal UA and
lead citrate do not stain callose at all, however it appears as
though it may according to what I believe is Callose in my
micrographs. Anyways if anyone has suggestions or comments please
email me.

Thanks!

---------------------------------------------------------------------------


From daemon Thu Aug 23 16:57:19 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 23 Aug 2001 16:55:44 -0500
Subject: for Tom Gore, U. Victoria

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Sorry to use the list for this, but my email to Tom Gore got bounced.
Tom -- your IT department doesn't believe you exist. I got this error
message when I emailed you:
The original message was received at Thu, 23 Aug 2001 16:49:49 -0500
from [144.92.45.161]

----- The following addresses had permanent fatal errors -----
{togo-at-uvvm.uvic.ca}

----- Transcript of session follows -----
.. while talking to smtp.uvic.ca.:
} } } RCPT To: {togo-at-uvvm.uvic.ca}
{ { { 553 5.3.0 {togo-at-uvvm.uvic.ca} ... Mail from 144.92.9.40
rejected(outputs);see http://orbz.org
550 {togo-at-uvvm.uvic.ca} ... User unknown

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Aug 23 20:37:49 2001



From: Greg Lum :      glum-at-sfsu.edu
Date: Thu, 23 Aug 2001 18:24:52 -0700
Subject: Re:

Contents Retrieved from Microscopy Listserver Archives
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We've had the same budgetary problems with oem contract maintenance
on our Philips CM120. We could only do 3 years of a 5 year
contract, renewable every year. At $16,000 to $18,000 for the
fourth and fifth years, our Dean bulked at the cost for what is
essentially an insurance policy, and so we didn't renew for the last
two years. Our choice was to go with an independent contractor, who
formerly worked for the OEM, and got good service. We still
couldn't do a service contract with him (less than half the OEM)
because our funding is very limited. Instead we used an open or
blanket $2500 service order. He at times bent over backwards to
help us, but without a service contract, he could not be "on-call."
Another limitation was a need for proprietary diagnostic software
that he no longer had access to. We had to go back to the OEM for a
one-time service.

The upshot is that you should try an independent service. Their
requirements will differ, but your alternative is to pony up $10-20
grand for "insurance". Maintenance on new microscopes that are
computer controlled maybe problematical for in-house training
especially when proprietary diagnostic software is needed. If
buying a new microscope, get as much funding for 5 years of service
as possible. We needed the 2 year warranty plus the 3 year service
contract before it performed steadily without problems. We're
starting into early middle age on our CM120 and experiencing
increasing problems. Our first TEM bought in 1962 lasted 30 years
and was used for teaching EM and research. We were able to operate
it without a service contract. I think we'll never see that kind of
performance again, but then they were much simplier machines.

--
Greg Lum
Lab Manager
Electron Microscope Facility
San Francisco State University
College of Science & Engineering
Ph: 415/338-1339
Email: glum-at-sfsu.edu


From daemon Thu Aug 23 21:52:29 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 23 Aug 2001 22:52:52 -0400
Subject: Re: OEM service vs. Insurance Companies---Long message

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Randy,
Thank you. This is quite interesting.

I'd like to respond from the viewpoint of one who worked for an OEM for
4 years and has subsequently had his own third party service company for
20 years.

First, the OEM is obligated to service contract customers first. You
have become a "billable" call and DO go to the bottom of the list. That
would not be all that different with a third party firm (although they
might try a little harder to be timely). The contract people have paid
up front (or have at least committed up front to pay) for an entire
year. This is guaranteed income and includes a guaranteed liability
(for the OEM) "to maintain the instrument/system to its original
specifications". Service organizations have to take this seriously.
This liability doesn't exist for billable customers. Work will be done
to the best of the OEM's ability and not beyond the limit of the
purchase order.

Second, the IC has no technical expertise, no intimate connection with
its customers and no obligation to use the manufacturer. I have been
called by at least one IC several times, but have never done any work
for them because they haven't a clue what I'm talking about when I tell
them I service SEMs but not TEMs and I like to stay within 500 miles of
home. (Their geography hasn't impressed me, either). They are trying
to emulate what happens when your car gets in a fender bender. There
are lots of people who can fix it, and many of them can do a pretty
satisfactory job. But ask yourself, "When my car has a rumble, or the
engine misfires and I need expert help or maintenance, who do I call?
My insurance company?" Probably not, not only because your insurance
doesn't cover that kind of thing, but because they couldn't help you
anyways. They know about insurance, not the inner workings of your car.

Third, any outside party that starts making commitments FOR the OEM (you
won't have to pay for recertification..............) I would turn away
from and run, not walk, run. If they tell you that THEY will pay for
recertification if you're unhappy and they will put it in writing,
that's another story.

As to the manufacturers making lots of money on their contracts, some
may, some may not. A lot depends on how well they are set up and run.
It also depends on the instrument density. If one engineer can service
20 or 30 systems within driving distance, the engineer is competent and
the service department backs him up well, yes, it can be quite
profitable. If you have to fly to every site, you're short on
experienced engineers and you don't support them well, you can lose your
shirt ( and have a whole lot of PO'd customers to boot).

Every time I've gotten one of those glib calls from an IC in Texas, in
the back of my mind a little voice is always saying "And just what makes
you think you're going to get paid?" I do thank you for confirming that
this concern is warranted.

The question now comes down to "What do you want?". The OEM has reason
to feel miffed. You want their expertise, their parts, their top level
response, their best guess on advance parts replacement for reliability
and you're going to pay someone ELSE the bonus if they do all this. The
IC is betting that the instrument will run reliably as it always has,
meaning very little cash out. The OEM no longer has the incentive to go
out of its way to insure reliability. They'll actually make more if
the system breaks more frequently. And as I pointed out, if they do
their best work, you pay someone else the bonus while they still have
the considerable expense of running a service department. I'm not
implying in any way that they will go out of their way to make it fail.
One of the advantages of sticking with the organization that actually
does the servicing is that you establish a raport with the personnel in
the field and in house. I'm only saying that they aren't going to go
out of their way to KEEP it from failing, because it's not in their
interest to do that. It IS in their interest when they have guaranteed
the performance for a full year.

Yes, generally speaking my service contracts are profitable, but there
is another major reason that I prefer to have systems on contract. It
becomes up to me how much time I spend on an instrument and how fast or
slow I work on it. Sometimes I'm very sure what the problem is and fix
it immediately, but often there are intermittent problems and I can tell
you that a billable customer watches the clock like a hawk when he sees
me sitting in front of a running system waiting for an intermittent
problem to show up. Sometimes there are subtle problems that the
customer isn't even aware of, yet. If the system is on contract I can
work without watching the clock and get everything right. In the long
run it saves me repeat trips. Also, if I should decide to let something
slide due to whatever circumstances, I know that I'm guaranteeing the
work and a later failure will be fixed at MY expense, not the customer's.

There was a time when people seemed more likely to reward good service
by paying a premium for it and showing a little loyalty to those who
provided it. The loyalty seems to be going by the board, and more and
more people want more for less, they aren't willing to pay for quality
because in their everyday life they buy cheap and when it breaks they
throw it out and buy cheap again. I don't believe that the scientific
instrument market will get there any time soon. This is specialty
equipment, capital investment, there for the long haul.. If it's well
taken care of , it will last for decades. That's where you can get the
savings.

If you were happy with the quality of the OEM's service and they are
willing to work with you, by all means see what you can work out! If
you weren't so happy with an OEM, see if there is a third party service
company that you can establish a good relationship with. The ICs can't
charge less, take out a middleman's cut, and give the organizations who
actually do the work and support the equipment, enough money to maintain
the quality you demand. If it looks too good to be
true...................................................

I sympathize with the cost-cutting that is being forced on you from the
bean-counters, but beware of false economies. Maybe one or more
instruments could be taken off contract, but what kind of grants are you
(the school and faculty) going to lose if it's down too much of the
time? What are the priorities of the organization? University service
labs are not supposed to be profit centers. They are SERVICE labs and
are heavily subsidized because the typical student (grad or undergrad)
can't afford to pay commercial rates for instrument time (especially
with what they're paying for tuition). If these instruments are
important to various departments then the decision is too important to
be made by some MBA who doesn't know the difference between hydrogen
embrittlement and cilia.

It would be interesting if we could get a response from the insurance
industry side of things.

My $.02 worth.

Sincerely,

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA



Tindall, Randy D. wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
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}
} As promised, here is a rundown on our OEM vs. insurance experiences. I
} decided to put this on the list based on the very large number of replies I
} received, some asking specifically that this be posted. As a result, I've
} removed all names, since I don't have a clue as to legal ramifications of
} being more specific. Sorry to be so wimpy, but in these cases I believe in
} CYA.
}
} The following obviously reflects our experiences alone, but based upon what
} I have heard from a few others, I don't believe that our situation is
} unique, by any means.
}
} Our lab, like many others, has been under increasing pressure to cut costs
} for the last two to three years, and since service contracts represent a
} significant proportion of our budget, they logically became a cost-cutting
} target immediately. It was beginning to appear that we were going to lose
} the contract on one of our TEM's to save money, especially since it wasn't
} heavily used. About this time one insurance company (IC, for short) began a
} series of high-powered presentations to the University purchasing people and
} administrators, promising significant cost reduction, with no reduction in
} services, choice of service providers, elimination of paperwork, etc.
} Facilities like ours were contacted by University purchasing staff in order
} to set up individual meetings to assess our needs and get estimates on the
} IC's costs versus OEM charges.
}
} After attending two open seminars and having two meetings with the insurance
} people and purchasing staff in our facility, the decision was made, with my
} agreement, to give the IC a shot at managing our service. The thinking was
} that we would try it for a year. The IC said we could back out at any time
} with no penalty, they would pro-rate our contracts to adjust for the
} differing ending dates for our OEM contracts, and, they said, there should
} be no "re-certification fee" if we decided to go back to OEM contracts,
} since we intended to use only the OEMs' engineers anyway. They would take
} care of the paperwork notifying the OEMs about the new arrangement, and all
} billing would be handled between the IC and the service providers. They
} would guarantee no increase in contract costs for, I believe, three years.
}
} The very first service call we made to the manufacturer on one of our TEMs
} resulted in our being told that they couldn't send an engineer, because we
} weren't on service contract any longer. When I explained that they were
} supposed to have been notified that we were with the IC, I was told that we
} would have to provide a University P.O. before anyone would be sent.
} Apparently, there was a substantial outstanding bill from this IC for
} service provided to a university in another state, and until it was paid no
} service would be provided on our contract. The IC's on-campus rep, our
} purchasing director, and the director of the university core facilities were
} notified by me immediately. Apparently some high level phone calling went
} on, because before the day was out, the service provider had called back
} saying the outstanding bill would be paid and an engineer was on the way.
} (Additionally, our purchasing director assured us that we could get a P.O.
} if necessary. We have terrific support here from purchasing and they have
} been invaluable on several occasions.)
}
} The engineer was sent out, the scope was back on line. BUT, it turns out
} that the promised payment was never made and we were back to square one the
} next time we needed service on that machine. In fact, it was worse, because
} the OEM was never paid for the service on our scope either.
}
} Our other scopes are from a different manufacturer and initially service was
} pretty much the same as before (although they made it clear they weren't
} happy about the switch). Response time was a bit slower, and there was more
} stinginess with replacement parts, but we had no real complaints.
}
} Then, the IC declared bankruptcy. Their campus reps disappeared, their web
} site went down, and they effectively vanished. We had already used part of
} the year's contract, but were only able to recover about $700 on the unused
} portion. Some labs lost thousands, I've been told. The service provider
} was never paid, and lost a substantial amount of money. (I'm told that this
} IC has reorganized under a different name and is soliciting new business,
} especially from universities. Ask lots of questions if a new company comes
} around. Be especially careful of large numbers of enthusiastic people in
} nice suits giving slick multi-media presentations!)
}
} We began discussing returning to our old OEM contracts and I was asked to
} get prices. The first thing I was told by one service provider was that we
} would have to have our instrument re-certified, which would cost about
} $1500, even though nobody had touched that scope but the OEM engineers.
} Needless to say, that rankled a bit, so we checked into another insurance
} company that had been recommended by several other labs. We were
} immediately impressed by the fact that they presented what seemed to be a
} more realistic picture of what they could do for us. They also offered
} significant savings (10-15%) over the old contracts, so we once again
} decided to give it a try.
}
} This time, the first call we made had the same result as before, i.e., the
} provider wanted a P.O. before an engineer would come out. When I asked why,
} they said that even though they had no problems with the new IC, they
} weren't going to take a chance on losing a ton of money again. If the
} university would sign a waiver accepting responsibility for paying any
} amounts in default, we could get service again. Fine, I said. Several
} days later the form was faxed to us and I forwarded it to our purchasing
} director, who responded that he had passed it along for review to the people
} who needed to sign off on it. That was last month sometime, and I have
} heard nothing yet. So far we have been waiting to get a preventive
} maintenance visit on this scope for about two months and nothing's in sight,
} yet.
}
} On our other scopes, we have again been able to get service, but now it
} seems that we are way, way down on the priority list. OEMs will tell you
} that they are obligated to service their contract customers first, and in
} our experience they certainly do. The service management (insurance)
} companies will tell you that this is not the case, that it's first-come,
} first-served. That has not been our experience. On one TEM we have been
} waiting weeks for a PM. It was scheduled twice and an engineer was here,
} but was pulled away on "emergency" calls, which I read as calls from
} contract holders. The PM has not yet been done. (Just minutes ago we
} received a call from our service engineer saying he's been pre-empted again
} and now he has NO idea when he can get here.) Our SEM has been serviced
} under the new arrangement in a relatively timely manner.
}
} Under the OEM contracts, service was provided "yesterday" and parts were
} replaced if they were even suspected to be bad. The working relationship
} was wonderful, and the service was stellar. We were called and reminded of
} PMs before they were due and we received periodic phone calls just to check
} on the status of our equipment. Under the new contracts, service is
} provided (if we can get it) whenever they get around to it, and engineers
} can be interrupted by calls to respond to contract holders. Parts are
} changed less frequently and are all billed. We schedule the PMs (no big
} deal). The relationship is much more tense than it used to be.
}
} We could probably increase the level of service by reminding the service
} people that when it's time to replace our scopes, service history will have
} a MAJOR bearing on whose machines we buy. However, I'm extremely reluctant
} to turn a previously very pleasant relationship into a confrontational,
} grudging one (although it seems to be headed that way, anyway). In
} addition, I can understand why some of these things are happening. If I put
} myself in the shoes of the OEM service managers, I'm not sure what I would
} do differently. On the other hand, I also have a tiny, nagging feeling that
} part of these problems are deliberately designed to pressure us back into
} our old contracts. That possibility (and I don't know it to be true) annoys
} me intensely, but I still haven't played the sales managers against service
} managers card. My sense is that OEMs make big bucks on service contracts
} and they take it VERY personally when we "fire them" as one service manager
} phrased it.
}
} I would like to emphasize very strongly that when field service engineers
} come out, they are uniformly excellent. They do everything they are allowed
} to do to provide top-notch care for the instruments. The problems
} originate higher up.
}
} In summary, we have found that service management companies do indeed save
} money on comparable contracts, but we have had a miserable time getting
} service at all when using them. This may not be typical---I don't know.
} Our level of service has declined greatly in terms of promptness, attitude,
} and parts supply. The attitude part was certainly not worth the extra
} several thousand dollars the OEMs wanted, but the promptness and parts
} issues probably were. Most disturbing was finding out that we could be
} denied service because of a problem originating at another university in
} another state that had absolutely nothing to do with us.
}
} We will continue for now with our new IC's to see if the problems correct
} themselves, especially since the problems didn't arise with this company.
} In addition, one of the OEM service managers called and suggested we might
} try a custom contract in which we could work with them to design our own
} service schedule. He said we could be informally trained during service
} visits to perform many of the maintenance chores on the scope and could
} retain a contract that would basically cover emergencies, at a substantial
} cost savings. We are looking into this now, because it seems to have lots
} of nice possibilities. (We already do filament/gun exchanges and cleanings,
} obviously, but I've never personally done a mechanical column alignment or
} routine maintenance requiring major disassembly.)
}
} My best advice: if you are currently with an OEM, stay there. If you are
} losing your local in-house service wizard and need to decide on contracts,
} go with the OEMs. Fight like hell to resist pressure to switch.
}
} If you are with an insurance company and are happy with them, stay there,
} and I wish you continued good luck. Please tell me your secret.
}
} My suggestion to OEM service providers: offer some in-house maintenance
} training courses, better maintenance manuals and schematics, and encourage
} customers to do more maintenance tasks (with appropriate provisos for
} covering dumb mistakes). Lower your contract prices so we're not so
} pressured to drop them. The glory days are over, folks. Time to compete.
}
} As for me, I have no financial interest in any of these folks. I just want
} our scopes to work. Feel free to contact me with any questions.
}
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
}
}



From daemon Fri Aug 24 06:12:43 2001



From: Christopher_R._Holp/FirstEnergy-at-firstenergycorp.com
Date: Fri, 24 Aug 2001 07:01:15 -0400
Subject: EDS equipment

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Peggy,

I would be willing to discuss some of my (limited) experiences on EDS
equipment, off list, if you don't mind.

Chris Holp
HolpC-at-firstenergycorp.com




From daemon Fri Aug 24 08:11:04 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 24 Aug 2001 09:03:32 -0400
Subject: RE: uranyl formate

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Hayat cites Brack, C.(1973), Experientia, 29:76 ("Use of uranyl formate
[0.5%] staining for the electron microscopic visualization of DNA-protein
complexes").

You might also check, Bremer, et al.(1994), J Mol Bio, 742:683-700, and
Steinmetz et al., M(1998), JMB,276:1-6.

Hope this helps,

Fred Monson


Frederick C. Monson, PhD
Frederick C. Monson, PhD
West Chester University of Pennsylvania
Center for Advanced Scientific Imaging (CASI)
Schmucker II Science Center (Room: SS024(Basement))
South Church Street
West Chester, PA, 19383
MailDrop: Department of Geology/Astronomy
email: fmonson-at-wcupa.edu
Phone: 610-738-0437




} ----------
} From: Chen Chen
} Sent: Thursday, August 23, 2001 3:14 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: uranyl formate
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Hi, everyone,
} Does anyone have the recipe and protocol on how to make uranyl formate
} solution for negative staining?
} And do you have any idea about whhich is better, uranyl aceate or formate?
} Thanks a lot.
}
} Chen Chen
}
} Dept of Biol. Chem.
} the Johns Hopkins Univ.
} School of Medicine
}
}
}


From daemon Fri Aug 24 08:32:56 2001



From: Zhu, Yimei :      zhu-at-bnl.gov
Date: Fri, 24 Aug 2001 08:28:45 -0500
Subject: POSITIONS AVAILABLE AT BROOKHAVEN NATIONAL LABORATORY

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POSITIONS AVAILABLE AT BROOKHAVEN NATIONAL LABORATORY


The Electron Microscopy Group at the Materials Science Division,
Brookhaven National Laboratory, is seeking experienced electron
microscopists and staff researchers in the areas of nano-scale electron
beam characterization of advanced materials. The group runs the
Electron Microscope Facility at BNL and has strong interaction with
scientists at BNL’s Physics Department and National Synchrotron Light
Source.

1) One research staff member

The Professional Research Staff will be responsible for the day-to-day
operation of the high-resolution JEOL 3000F (FEG/TEM/STEM) microscope.
The instrument has a wide range of attachments with energy filtering,
EELS, EDS, holography, Z-contrast and in-situ cooling and heating
capabilities. Its data acquisition system includes four CCD cameras and
a Fuji Imaging Plate unit. A degree of M.A. or Ph.D in physics or
materials science with strong background in electron microscopy is
required for the successful candidate. Salary will be commensurate with
education and experience.


2) Three Research Associates / Postdocs in the following areas

* Measurements of charge density distribution and chemical bonds using
quantitative electron diffraction and synchrotron x-ray techniques

* MBE film growth of nanostructured functional materials and near-edge
fine structure of electron energy-loss spectroscopy

* Phase retrieval using various methods including off-axis electron
holography to study local distribution of electrostatic potential and
magnetic induction in crystals and defects

* Study of atomic and electronic structure of interface of functional
materials by comparing experiments with theory

* Electronic structure calculations of crystals and defects using
density functional theory and molecular simulation methods

Successful candidates for the three positions will be Ph.D graduates in
physics or materials science with a sound background in the relevant
areas and associated computer knowledge. Prior experience using
transmission electron microscopy is essential only where explicitly
stated. The positions are for one year initially, normally renewed for
a second year with an excellent chance of conversion to scientific
staff. The above postdoctoral candidates are also eligible to apply
for BNL’s Goldhaber Fellowship (for details, see
http://www.bnl.gov/bnlweb/pubaf/goldhaber.htm).



Positions will remain open until they are filled. Please send your
resume and three references to
Dr. Yimei Zhu, Materials Science Division, Brookhaven National
Laboratory, Upton, Long Island, NY 11973 (e-mail: zhu-at-bnl.gov). BNL is
a multipurpose national laboratory managed by Brookhaven Science
Associates for the U.S. Department of Energy. BNL is an equal
opportunity employer committed to building and maintaining a diverse
work force.



--
==================================
Yimei Zhu
Building 480
Dept. of Applied Science
Brookhaven National Laboratory
Upton, New York 11973
*Tel. (631)344-3057
*Fax. (631)344-4071

*please note the new area code
==================================




From daemon Fri Aug 24 08:52:00 2001



From: werner-at-rosharon.oilfield.slb.com (Andrew Werner)
Date: Fri, 24 Aug 2001 08:46:56 -0500
Subject: Re: SEM-EDS: Help with choosing a new system

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At 01:13 PM 8/23/2001 -0500, Macatangay, Peggy J., Celanese/US wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have a PGT EDS / imaging system on and ISI DS130. The PGT sales people
are very nice, the applications folks helpful, and their service man is
highly competent - but I truly regret buying their system. The short
version of the story is that this particular system (from 1997) uses a PC
front end to run the Sparc board that really runs the EDS and imaging
software, and the PC and Sparc *hate* each other. This is a cumbersome,
frustrating construct; maybe later versions are less unsatisfactory.

If anyone wants to know more please let me know off-list, as I am
uncomfortable being so negative in a public forum. Unless that is bad
netiquette - maybe it is unprincipled to take it to private e-mail, and
ought to be said in plain view. Whatever...

Disclaimer: These comments are my personal observations and opinions and do
not reflect or represent the views (if any) of my employer.

Regards,
Andrew T. Werner
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273



From daemon Fri Aug 24 10:16:17 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 24 Aug 2001 09:46:09 -0500
Subject: Re: SEM-EDS: Help with choosing a new system

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Ken,

Great posting. Lots of food for thought.

As was pointed out to me by another person, I neglected to discuss
third-party service providers. This is mainly because I have no experience
with them, but they are increasingly becoming an attractive alternative.
The bulk of my posting, I think, argued for retention of OEM contracts
whenever possible, for many of the reasons you discussed below. In our
experience, the difference in service between insurance and OEMs is
incredible.

That said, however, it is still a mystery to me why OEMs seem to consider
billable work at $200 or more per hour, including travel time, plus per
diem, mileage, hotels, meals, etc. to be some kind of sacrifice that
deserves punishment. To me that seems like a pretty good bonus over what
they are likely to make under a service contract. One recent visit to
service our FESEM resulted in charges of well over $7000, mostly to work
with software glitches! Two more visits like that and we will have paid for
the OEM contract and the OEM will have increased their income over what a
contract would have brought them. Maybe it's simply that money up front is
preferable, as you say, since that eliminates the risk of not being paid at
all.

If working on a billable basis really is a hardship for OEMs and we're told
we can't afford their service contracts, then that's where the free market
comes in and third-party engineers have found their niche. People like you
fill that gap admirably. I expect life isn't always easy, though,
especially when you must rely on OEMs for parts and specialized expertise.
One third-party provider told me of losing thousands of dollars when an OEM
changed the price of a part upon finding out that it was ordered by an
independent service provider. I've also heard that sometimes OEMs try to
avoid selling parts to independents. Have you had any experiences along
these lines?

It seems that the entire service landscape is changing and everyone is
scrambling for alternatives. Makes for interesting discussion.

Randy

-----Original Message-----
} From: Ken Converse [mailto:qualityimages-at-netrax.net]
Sent: Thursday, August 23, 2001 9:53 PM
To: Tindall, Randy D.; MSA, listserver


As much in any area, it is important to just get your hands on a unit and
try it out and see if it will do what you want in the way you want. Most
systems will have the capabilities you want, but sometimes there are
restrictions or encumbrances that can build up frustration over time.

I believe there is also an issue of corporate heritage and influence. As a
rule, larger, more established companies _should_ have and devote the
resources necessary for new developments. They should have good, polished
products which continue to improve. There should also be good support
behind those products over many years. Smaller, newer companies are
generally innovative and should be expected to come up with new product
ideas, but the execution of those ideas might be a little rough. They do
not have the resources to polish everything nicely. There might also be
issues of support over the long run. There is also a good chance that a
smaller company might be swallowed up by a larger one.

This seems to be proving true in the EDS field, but there are exceptions to
some of these principles with almost every company. Some you would expect
to deliver more but they don't, and vice versa. We currently have an Oxford
ISIS and an IXRF EDS system. We also had a Kevex Delta (became part of
Noran) and a Tracor TN-2000 (became Noran) systems. We looked at EDAX and
PGT and they had good systems, but we made other choices. That has been a
few years ago. I would have to investigate the field thoroughly if I were
making the decision again.

I don't think that we regret any of the decisions that we made under the
circumstances of the time. We have been satisfied with the systems we
chose. I suppose you could say that in various ways, the companies did not
keep up or made some strategic choices of their own and we opted for
different systems when it came time for replacement. (Some of that is now
ancient history. For example, Tracor used a proprietary operating
environment on its 5000 series of analyzers and that was a major negative
for us. They have since gone more standard.) Sometimes it was an issue of
price-performance ratio for the features we were getting.

So I come back to my earlier position.
-Find out the capabilities of the current systems. (Qual, Quant, Imaging,
Mapping, Linescans, etc.)
-Determine which of those are really important to you and necessary.
-Evaluate your options for those functions for accuracy AND usability and
price. Be sure to get your own hands on the units during the demos. It make
take a couple looks at the models to give a fair look.
-Then make your choice.

Happy hunting.
Warren

At 01:13 PM 8/23/2001 -0500, you wrote:

} I am investigating EDS systems to integrate with a JEOL-6100 SEM. I'm
} leaning towards PGT's Spirit system. Other considerations are Oxford's INCA
} and Thermo Noran's Vantage. Does anyone have particularly positive or
} negative experience with PGT? I don't often hear much about that company.
} Any comments about the other possibilities would be appreciated, as well.
}
} Thanks!
}
} -Peggy
}
} Peggy McKarns Macatangay, PhD
} Project Analyst 2
} Celanese Corpus Christi Technical Center

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Aug 24 10:31:23 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 24 Aug 2001 08:28:06 -0700
Subject: Re: LM - Something I just don't get

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Regarding this subject, the local Zeiss rep gave me a
copy of an interesting and valuable article that hits
right on the topic. The article is "Choosing Objective
Lenses: The importance of numerical aperture
and magnification in digital optical microscopy."
Piston, D. (August, 1998). The Biological Bulletin, 195/1.

Anyone interested should look this up. It also talks
about digital capture and the relationship to laser
scanning confocal microscopes.

gary g.



From daemon Fri Aug 24 11:25:51 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Fri, 24 Aug 2001 13:19:02 -0300
Subject: HMDS recipe: summary replies - longish

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Sorry I am so long in posting this but a strange and wonderful thing
intervened - summer vacation.

Below are three of the responses I received on the question of HMDS. We
used the following protocol and the sample seems to show good morphology.
The gross morphological features I think are probably affected by mechanical
forces when you remove "flimsy" structures from any liquid. The ciliated
structures appear well preserved.

Every step of the protocol is done in a fume hood especially for the HMDS,
which is a strong irritant.

A. After the fixation (in 1G4F), the dehydration begins with two baths of
one hour for each step.
1. PO4 wash
2. 50% ethanol
3. 70% ethanol
4. 80% ethanol
5. 95% ethanol
6. 100% ethanol

B. Transition ethanol-HMDS
1. 100% ethanol: HMDS 1 - 30 minutes
2. 100% ethanol: HMDS 2 - 30 minutes
3. 100% ethanol: HMDS 3 - 1 hour

C. Pure HMDS
1. HMDS 1 - 1 hour
2. HMDS 2 - 1 hour

D. The samples are placed fresh HMDS in a dessicator with silica gel in the
bottom overnight. They are ready to be dissected.

Celine Barre is the researcher looking at the specimens.

"O'Neil, David" wrote:
}
} We have been critical point drying scallop specimens successfully for SEM
to
} examine them as they grow. We are now at a point where the specimens no
} longer fit in the CPD chamber. With only a couple of specimens to go we
} thought to try HMDS. The samples are approximately 30mm in diameter.
Does
} someone have a protocol they have used for larger specimens that has
worked
} for them? You can send them directly to me and I can summarize for the
} list. Thanks in advance.

############################################################################
########

Hi David,
I have not worked on scallops but I do regularly work on fleshy
invertebrates -
polychetes, snails, insects etc.
My method is to take the specimen through OH dehydration series 50, 70, 80,
90,
100, 100%. At each stage I leave the specimen in for a few hours. Because
the
specimen is large in diameter and quite thick (5mm).
Then 50% HMDS + 50%OH for several hours, I do change the solution 2 to 3
times
during this period.
Then finally into fresh 100% HMDS for several hours.
To dry I remove the specimen from the HMDS and place it in a petri dish that
has
filter paper on the bottom of it which is lightly soaked with HMDS. The lid
sits
over the top - this ensures that the drying is slow and controlled. If the
drying is too rapid I find that some specimens distort.
Oh all of this procedure must be done in a fume hood.
Another little bit is that when soaking in 50% and 100% HMDS be careful when
removing the lid. There is often a gas buildup in the bottle. I think the
gas is
NH4???? Not very pleasant.
Also, you might find that the scallop is too thick for successful
penetration
from both HMDS and OH. If this happens then you might have to consider
halving
the specimen so to reduce the thickness thus allowing better penetration.
I hope this helps???

Have fun

Sue


--
Sue Lindsay

SEM Laboratory Manager
Scanning Electron Microscope Unit
The Australian Museum ph 02 9320 6198
6 College st fax 02 9320 6059
Sydney, NSW, Australia Email suelind-at-austmus.gov.au

############################################################################
########

Hi David
I don't know what CPD you have available, but I've dried crocodile
pharyngeal specimens (ca. 5X8X1.5cm) quite successfully in a
BioRad CPD Jumbo. I remove the basket holder from the CPD
chamber and fill up with Eth-OH while tilting the apparatus, door
uppermost and then transfer the specimens into the chamber and
seal it. Specimens are "manipulated" onto the floor by tilting the
whole bomb back and fore. Drying is done over an extended period
of about 3.5 days. Naturally a large volume of CO2 is used flushing
every ±2-3h through the day. I have a circulating waterbath and do
the run at 10oC.

I'm interested to see how other folk overcome large specimen
drying.

Regards
John

Mr John F. Putterill
Electron Microscopy Unit Tel: (Int) 27-12-529-9175
Pathology Section Fax: (Int) 27-12-529-9165
Onderstepoort Veterinary Institute E-mail: john-at-moon.ovi.ac.za
Private Bag X05 http://www.ovi.ac.za
Onderstepoort 0110
South Africa

############################################################################
########

David,

I've been watching the listserver for several days for replies to
your
inquiry, and haven't seen any. I've been using HMDS since the 1980's,
and have found that it works well within certain constraints. It is not
something to use if you need to preserve the size of a sample, as it
causes shrinkage of tissues, particularly of connective tissues, that is
not seen to a great extent with critical point drying. HMDS is caustic,
and flammable, and should be used only in a fume hood by someone with
gloves on. It produces samples with good surface morphology, similar to
CPD. With samples such as you are talking about, you will need extensive
time to infiltrate your scallops in order to replace your dehydrating
fluid (ethanol) with the HMDS. Otherwise, you will find that the ethanol
will still be in the center of your sample and will not give you good
drying results. If I were doing your project, I would try to slice the
scallops in cross section into several pieces so the fluids you use can
get in and out of the tissue more easily, or cut tissue out of the
bottom of the scallops (since this side will be mounted on your sample
holder and not used for imaging). Make sure to dispose of the waste HMDS
in a sealed container so the highly irritating fumes don't get out in
your lab.

For tissue like rabbit ovaries, I bisect the ovaries longitudinally
after glutaraldehyde fixation. These ovaries are 1.5cm long, but only
0.5cm in diameter. It is not necessary to use osmium tetroxide for HMDS
preps. I then rinse my tissue for 15 minutes in buffer, then 15 minutes
in 0.9 % salline, the idea here being to slowly change the osmotic
pressure seen by the tissue. I use phosphate buffer, and so must then go
through 3 10 minute rinses in distilled water to insure complete removal
of phosphate salts from the tissue. If this step is skipped , the salts
will precipitate on the outside of the tissue when drying in HMDS, a
phenomenon I didn't see when doing CPD.

Following the water rinse, I dehydrate the tissue in changes of
ethanol, 15-30 minutes per change, in 35%, 70%, 95%, and two half hour
changes in 100%. I infiltrate my samples with 100% HMDS, 2 times, for a
minimum of 10 minutes each time. Following infiltration, I remove the
HMDS from my sample vial and then shake my samples out on a paper towel
or filter paper so they will dry, moving them several times so all sides
dry quickly. The tissue will turn white colored as it dries, a process
that can take up to a minute for larger samples. I work with all of my
solutions at room temperature, and do all of my work in the hood. For
dense samples such as the ovaries, or your scallops, once the sample
appears visibly dry I transfer it to a 60 Degree vacuum oven for several
hours or overnight to insure that the sample is completely dry before
sputter caoting it for the SEM. If you don't have access to a vacuum
oven, a vacuum is still better than letting the sample remain at
atmospheric pressure to dry.

HMDS is said to harden the tissue while it is infiltrating it after
ethanol dehydration. I don't know if the chemical interaction of HMDS
and acetone or methanol will produce the same hardening effect. This is
why I only dehydrate with ethanol.

I envy people like you that get to work with edibles. I met some
people
in Florida that study reproduction in Florida lobsters, and others that
study the same thing in grouper. These poor folks have to "discard" the
carcasses of their study subjects after removing their gonads. Tough
life, but somebody has to do it! I only get to work with human and lab
animal tissue. Oh well! Let me know if this helps you. Feel free to
write with other questions if you need to.

Edward Haller
Lab Manager
Diagnostic Electron Microscopy
University of South Florida
Pathology Department
(813)974-9584


From daemon Fri Aug 24 13:02:46 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Fri, 24 Aug 2001 13:52:24 -0700
Subject: callose labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi matthew,
There is a monoclonal antibody commercially available that recognizes
callose (1-3)-B-glucans), if you want to do immunogold labeling.
It's easy to use - it doesn't care if the tissue is embedded in Epon (Embed
812) or if it has been fix with osmium. The company is Biosupplies
Australia. Visit their web site for more info www.biosupplies.com.au The
product is Cat # 400-2.
it's a good one so you should have good luck with your project,
Beth

} Subject: Ask-A-Microscopist:stain for b eta glucans mainly callose

} Email: mcs4-at-dana.ucc.nau.edu
} Name: Matthew Salanga
}
} Organization: Northern Arizona University
}
} Education: Graduate College
}
} Location: Flagstaff, Arizona
}
} Question: I have been doing TEM work on plant cells for my thesis. I
} was wondering if anyone might have a suggestion on a stain which
} would be specific for
} beta glucans mainly callose. Literature suggests that normal UA and
} lead citrate do not stain callose at all, however it appears as
} though it may according to what I believe is Callose in my
} micrographs. Anyways if anyone has suggestions or comments please
} email me.
}
} Thanks!


******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************~




From daemon Fri Aug 24 14:12:16 2001



From: Ingram, Mike :      MIngram-at-rodel.com
Date: Fri, 24 Aug 2001 14:58:07 -0400
Subject: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Not a serious question here, more of a curiosity.

I use EDS when talking about x-ray analysis. I see many using EDX. Which
is correct?

Mike Ingram


From daemon Fri Aug 24 14:52:45 2001



From: Bob Roberts :      bobrobs-at-earthlink.net
Date: Fri, 24 Aug 2001 12:51:18 -0700
Subject: Re: SEM-EDS: Help with choosing a new system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peggy,

I think any EDS system search would be somewhat incomplete
without taking a look at and evaluating some of the smaller
companies that provide PC based systems coupled with new EDS
detectors. In the final analysis, it seems to come down to a
system that best fits your application and needs, ease of
usability and performance. Last but certainly not least is
future support.

Some of these companies that come to mind would be WinEDS by
TNAS, 4pi Analysis, and Emispec Systems. There are, of
course, others that provide these systems. Sometimes dealing
with smaller companies can be an advantage.

Good Luck,

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
480.967.3946


Warren E Straszheim wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} As much in any area, it is important to just get your hands on a unit
} and try it out and see if it will do what you want in the way you want.
} Most systems will have the capabilities you want, but sometimes there
} are restrictions or encumbrances that can build up frustration over time.
}
} I believe there is also an issue of corporate heritage and influence. As
} a rule, larger, more established companies _should_ have and devote the
} resources necessary for new developments. They should have good,
} polished products which continue to improve. There should also be good
} support behind those products over many years. Smaller, newer companies
} are generally innovative and should be expected to come up with new
} product ideas, but the execution of those ideas might be a little rough.
} They do not have the resources to polish everything nicely. There might
} also be issues of support over the long run. There is also a good chance
} that a smaller company might be swallowed up by a larger one.
}
} This seems to be proving true in the EDS field, but there are exceptions
} to some of these principles with almost every company. Some you would
} expect to deliver more but they don't, and vice versa. We currently have
} an Oxford ISIS and an IXRF EDS system. We also had a Kevex Delta (became
} part of Noran) and a Tracor TN-2000 (became Noran) systems. We looked at
} EDAX and PGT and they had good systems, but we made other choices. That
} has been a few years ago. I would have to investigate the field
} thoroughly if I were making the decision again.
}
} I don't think that we regret any of the decisions that we made under the
} circumstances of the time. We have been satisfied with the systems we
} chose. I suppose you could say that in various ways, the companies did
} not keep up or made some strategic choices of their own and we opted for
} different systems when it came time for replacement. (Some of that is
} now ancient history. For example, Tracor used a proprietary operating
} environment on its 5000 series of analyzers and that was a major
} negative for us. They have since gone more standard.) Sometimes it was
} an issue of price-performance ratio for the features we were getting.
}
} So I come back to my earlier position.
} -Find out the capabilities of the current systems. (Qual, Quant,
} Imaging, Mapping, Linescans, etc.)
} -Determine which of those are really important to you and necessary.
} -Evaluate your options for those functions for accuracy AND usability
} and price. Be sure to get your own hands on the units during the demos.
} It make take a couple looks at the models to give a fair look.
} -Then make your choice.
}
} Happy hunting.
} Warren
}
} At 01:13 PM 8/23/2001 -0500, you wrote:
}
} } I am investigating EDS systems to integrate with a JEOL-6100 SEM. I'm
} } leaning towards PGT's Spirit system. Other considerations are
} } Oxford's INCA
} } and Thermo Noran's Vantage. Does anyone have particularly positive or
} } negative experience with PGT? I don't often hear much about that
} } company.
} } Any comments about the other possibilities would be appreciated, as well.
} }
} } Thanks!
} }
} } -Peggy
} }
} } Peggy McKarns Macatangay, PhD
} } Project Analyst 2
} } Celanese Corpus Christi Technical Center
}
}
} ----------------------
} Warren E. Straszheim
} Materials Analysis and Research Lab
} Iowa State University
} 23 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of
} materials
} Computer applications and networking
}
}
}




From daemon Fri Aug 24 15:01:23 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 24 Aug 2001 14:56:37 -0500
Subject: Re: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
The technique is "energy-dispersive spectroscopy" (in
contrast to wavelength-dispersive spectroscopy). In formal writing,
there is little reason to abbreviate these terms, but of course folks
do. It is possible that EDX describes a proprietry instrument that
some OEM named in this way.

Hope this helps,
Tobias (no problem is too small to baffle me) Baskin



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Fri Aug 24 15:16:20 2001



From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 24 Aug 2001 15:10:27 -0500
Subject: Re: XEDS aka EDS or EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually to be consistent I ALWAYS use....


XEDS = X-ray Energy Dispersive Spectroscopy

which is completely analogous to

EELS = Electron Energy Loss Spectroscopy

to my mind it is the most logical since EDS does not
say what you are energy dispersing and EDX is not
quite a full descriptor.

However, EDS, EDX, EDXS are used often in
the literature.

Nestor
Your Friendly Neighborhood SysOp
--
===========================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4289
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From daemon Fri Aug 24 15:55:21 2001



From: max.sidorov-at-amd.com
Date: Fri, 24 Aug 2001 13:49:56 -0700
Subject: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Either one is correct. It's a matter of taste.
EDXS would be more correct (Energy Dispersive X-ray Spectroscopy).
Williams&Carter suggest yet another one - XEDS. I use EDX.

Max Sidorov
AMD

-----Original Message-----
} From: Ingram, Mike [mailto:MIngram-at-rodel.com]
Sent: Friday, August 24, 2001 11:58 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Not a serious question here, more of a curiosity.

I use EDS when talking about x-ray analysis. I see many using EDX. Which
is correct?

Mike Ingram




From daemon Fri Aug 24 16:07:46 2001



From: JLaGoy :      JLaGoy-at-bodycote-imt.com
Date: Fri, 24 Aug 2001 17:04:13 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe - My apologies, but I do not have instructions for the proper
way to unsubscribe, just how to post messages.

jlagoy-at-bodycote-imt.com


From daemon Fri Aug 24 16:39:51 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 24 Aug 2001 17:33:13 -0400
Subject: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have always used EDS because of the counterpoint to WDS. But I always define its first use as Energy Dispersive X-ray Spectroscopy. Now, I am going to start using Nestor's convention and start referring to both of them as
X-ray Energy Dispersive Spectroscopy (XEDS) and
X-ray Wavelength Dispersive Spectroscopy (XWDS). Hey I have an idea --let's start a convention!

BTW, it should never be EDAX. That's the same as calling all photocopies, Xeroxes.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ingram, Mike [mailto:MIngram-at-rodel.com]
Sent: Friday, August 24, 2001 2:58 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Not a serious question here, more of a curiosity.

I use EDS when talking about x-ray analysis. I see many using EDX. Which
is correct?

Mike Ingram


From daemon Fri Aug 24 17:56:29 2001



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 24 Aug 2001 18:50:33 -0500
Subject: Re: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used what I believe is the generic version of XEDS, x-ray
energy dispersive spectroscopy.


At 2:58 PM -0400 8/24/2001, Ingram, Mike wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Fri Aug 24 18:29:48 2001



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 24 Aug 2001 18:23:40 -0500 (CDT)
Subject: Additional confusion Re: EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When I was shopping for one, I was corrected by someone along the way that
the "S" in EDS stands for spectrometry, not spectroscopy. Several books
here in the lab library also use that term.

Before I confuse part of the next generation of microscopists this
upcoming semester, is one term better than the other - or is it a potato,
potatoh, tomato, tomahtah (let's call the whole thing off) kind of thing?

Heather Owen


Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Fri Aug 24 19:19:46 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 24 Aug 2001 17:12:18 -0700
Subject: Re: Ask-A-Microscopist: how to suspend a spider in a clear cube

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John:

The best material we've found to use is a polyester mounting media such as our
Polymet material. In fact, I just did exactly that for some office "fun"
around here. We found a nice little black widow that ended up making an even
nicer paperweight. I will send you information off-line on that product.

Best regards-

David

john_bruss-at-bose.com wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (john_bruss-at-bose.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} August 22, 2001 at 17:00:52
} ---------------------------------------------------------------------------
}
} Email: john_bruss-at-bose.com
} Name: John Bruss
}
} Organization: Bose Corp.
}
} Education: Graduate College
}
} Location: San Diego, CA, USA
}
} Question: What materials and process would an amateur use to suspend
} a spider in a clear cube for unmagnified artistic and historic use?
} Where are those materials available in small quantities?
}
} ---------------------------------------------------------------------------

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Fri Aug 24 21:45:34 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Fri, 24 Aug 2001 19:34:31 -0700
Subject: Re: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This name problem has been around for 30 years! Back in 1971 John Russ
edited a little book called Energy Dispersive Analysis of X-rays published
by the ASTM. Then Nuclear Diodes, Inc, renamed themselves EDAX and the
competition could not use that trademarked name. John worked for EDAX and
promoted the technique as "EDAX". Kevex had a book published in 1973 called
"Everything you wanted to know about XES (X-Ray Energy Spectrometry)" but
that name did not stick. EDS and EDX have stuck and are the most popular. I
once wrote a paper on filter use for "EDXRF" (Energy Dispersive X-Ray
Fluorescence) and it did not get listed in abstract indexes of X-Ray papers
because they did not recognize the name! I think EDS is a bad name, but I
use it because others understand what I mean.

Ronald Vane
XEI Scientific.





From daemon Sat Aug 25 15:05:38 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 25 Aug 2001 15:57:15 -0400
Subject: Re: Which is Correct, EDS or EDX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
My guess is that either will do the trick, but I would take EDX as a
shortened version of Energy Dispersive Analysis of X-rays, which, of
course, is EDAX. I've also seen EDXA which would keep you out of
trouble with EDAX. One really is doing a specific type of analysis,
spectroscopy, which would cause me to lean towards Energy Dispersive
Spectroscopy.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ingram, Mike wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Not a serious question here, more of a curiosity.
}
} I use EDS when talking about x-ray analysis. I see many using EDX. Which
} is correct?
}
} Mike Ingram
}
}
}



From daemon Sat Aug 25 21:45:13 2001



From: Gordon Couger :      gordon-at-couger.com
Date: Thu, 12 Jul 2001 14:04:42 -0500
Subject: Amateur Scientist CD-ROM

Contents Retrieved from Microscopy Listserver Archives
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While this is off topic I am getting enough email from on the subject I
think has enough interest to the group that I should post the particulars on
sources for the
CD-ROM. My only interest in the project is I suggested the idea to Shawn
Carson and I am very glad that they carried it out. It has all the Amateur
Scientist articles
from the 20's until very recently.

I got mine from CD-ROM http://www.surplusshed.com/ the Surplus shack for $39
dollars. Fry's Electronics is a west coast electronics chain
http://www.frys.com/
they don't sell from their web but the Sunny Vale store does sell mail order
their phone number is (408) 617-1300. Fry's has them for $25 dollars and I
have been
told that they have a mail in $25 dollar rebate that comes with it.

Gordon

Gordon Couger
Stillwater, OK







From daemon Sat Aug 25 23:49:26 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sun, 26 Aug 2001 00:43:22 -0400
Subject: LM, Workshop on use of the Microscope

Contents Retrieved from Microscopy Listserver Archives
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There are still a few places left for he New York Microscopical Society
Workshop listed below:
N.Y.M.S. Bernard Freidman Memorial Workshop
Use of the Microscope, September 15,22,29,Oct. 6,2001,10AM to 4PM

A basic course on light microscopy which will cover the following topics: T
heory of microscopy, Kohler Illumination, Diffraction Theory, Contrast
Methods , Polarized light, Phase Contrast, Interference, Hoffman
contrast, Rheinberg, Dark-field & oblique Illumination, etc.
The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of microscopy.
The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of
Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR
Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHERE: 30 3. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 (
parking, accessible by public transportation, Information on car pools and
transportation will be provided.)
COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.HOW:
Register using the form below. Limited to the first 12 registrants.Send form
to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.
FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 E-mail
donoleary-at-worldnet.att.net Fax (425) 988-1415

PLEASE POST
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From daemon Mon Aug 27 09:47:01 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 27 Aug 2001 10:26:54 -0400
Subject: Re: Additional confusion Re: EDS or EDX?

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When I was shopping for one, I was corrected by someone along the way that
the "S" in EDS stands for spectrometry, not spectroscopy. Several books
here in the lab library also use that term.

Before I confuse part of the next generation of microscopists this
upcoming semester, is one term better than the other - or is it a potato,
potatoh, tomato, tomahtah (let's call the whole thing off) kind of thing?

Dear Heather,
The "scopy" part has to do with observation, whereas the "metry" part
has to do with measurement (i.e., quantitation), so energy-dispersive
spectroscopy is separating the photons by energy in order to look at the
spectrum, and E-D spectrometry is separating the photons in order to
perform measurements. I can understand a vendor insisting that the
instrument is capable of quantitation and is, thus, a spectrometer.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Aug 27 16:33:22 2001



From: Tyler Gruber :      tcgruber1-at-excite.com
Date: Mon, 27 Aug 2001 16:26:03 -0500
Subject: Quantimet 970 and camera

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We no longer need our Quantimet 970 Image Analysis System interfaced with an
intensified Vidicon – type camera. The camera was bottom-mounted on a TEM.
We are the original owners, have kept it well serviced (contract), and have
used this system up until the last year. Interested parties please contact
tcgruber1-at-excite.com offline.

Regards,

Tyler Gruber





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/


From daemon Tue Aug 28 07:45:55 2001



From: David Spector at Cold Spring Harbor Laboratory :      spector-at-cshl.org
Date: Tue, 28 Aug 2001 08:36:10 -0400
Subject: Gridded Thermonox Coverslips

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Hi,

Does anyone know if gridded coverslips are available made out of Thermonox?

Thanks,

David Spector
--
Dr. David L. Spector
Cold Spring Harbor Laboratory
One Bungtown Road
Cold Spring Harbor, New York 11724
Tel. (516) 367-8456
Fax (516) 367-8876
email: spector-at-cshl.org


From daemon Tue Aug 28 07:51:14 2001



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Tue, 28 Aug 2001 05:49:41 -0700
Subject: Re:ELMDAS COMPANY

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Dear List,

I was puzzled to hear that a member has had difficulty contacting John
Best at Elmdas. I am a happy customer of John's and have never had any

difficulty contacting him by phone or e-mail. He replied to my e-mail
to him last Friday within hours.

John's correct e-mail address is } jbest-at-elmdas.com {

Bart Cannon
Cannon Microprobe
Seattle



From daemon Tue Aug 28 09:06:50 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Tue, 28 Aug 2001 09:04:23 -0700
Subject: Re: Which is Correct, EDS or EDX?

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A post-script on the "EDX" vs. "EDS" discussion.

A couple of years ago, our company was contacted by lawyers from
"Electronic Data Systems" (EDS) who told us that we were violating their
trademark by offering a product called "The Personal EDS". I personally
thought this was nonsense, but it was going to cost us a lot of money to
pursue this, even if we were able to prevail.

So I now use EDX because: (1) it is recognized; and (2) I don't much
like talking to lawyers.

If it were up to me, I would much prefer "XES" (which as Ron Vane
pointed out, was the name Kevex used to use). "X-ray Energy
Spectroscopy" may not be completely self-explanatory, but at least it is
not misleading. The use of the word "dispersive" in this context is
just plain wrong! (you are not "dispersing" energy) EDS does have a
"symmetry" with WDS, but it is an inappropriate one IMHO.

Fred Schamber
ASPEX, LLC

"Ingram, Mike" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Not a serious question here, more of a curiosity.
}
} I use EDS when talking about x-ray analysis. I see many using EDX. Which
} is correct?
}
} Mike Ingram


From daemon Tue Aug 28 10:20:44 2001



From: Xudong Fan :      fanx-at-msu.edu
Date: Tue, 28 Aug 2001 11:26:33 -0400
Subject: Philips CM-10 TEM available

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An excellent Philips CM-10 transmission electron microscope
is being offered for sale by Michigan State University.  It
is 1987 vintage and has been on service contract since new.
Asking price: $10,000. Please contact Dr. Xudong Fan for more
detail, fanx-at-msu.edu, 517-353-4525.

Yours Sincerely,
Xudong Fan, Ph.D.
Center for Advanced Microscopy
Michigan State University
East Lansing, MI, 48824


From daemon Tue Aug 28 11:22:46 2001



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Tue, 28 Aug 2001 11:17:39 -0500
Subject: Link eXL EDS system

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List,

Looking for a replacement RGB monitor for my LINK eXL system. Oxford has
been contacted and due to the "very high" price to get a replacement "for
this rare jewel" I am exploring other options. I know the power supply is
damaged because of all the burnt components but am not sure what else may be
damaged. Fix may take a while, so I'm hopeful someone may have a retired
system with a monitor I could deal for. Interest from third party EDS repair
companies who would like to tackle this beast would also be useful.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu



From daemon Tue Aug 28 12:57:11 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Tue, 28 Aug 2001 14:08:49 -0400
Subject: RE: uranyl formate vs. uranyl acetate

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Chen Chen:
Uranyl formate, in my experience using it as a negative stain to reveal
the finest detail of virus structures at very high mags, gave superior
results to uranyl acetate. In short, there appeared to be a finer grain
detail and a better resolution of the virus's structure.
A word of caution: uranyl formate should be used within about 15 minutes
after making it as it tends to readily form a precipitate. For general work
where I'm not trying to obtain the ultimate detail, I stick to uranyl
acetate simply to avoid the precipitate problem.
My protocol is to make up a 1% sol. in dd water and immediately give a
brief spin in a centrifuge. Some have simply filtered it with a Whatman
filter. Draw off just the top portion for staining. I find a final
solution of
0.5% works ok.
I place a drop of my virus solution on parafilm, and after a few minuets
wait, touch my grid to the surface to pick up the phage. I find that the
phage tends to collect on the surface of the drop. After removing most of
the phage containing drop I apply a drop of stain or, if you wish to
"cleanse" your sample prior to staining, you may apply a drop of a 1 mM
solution of ammonium acetate in dd water for a very brief period. Depending
on the sample, I've sometimes follow up with another brief application of a
drop of dd water. The uranyl formate negative stain is applied and all but
a trace immediately removed.
Keep in mind that beam exposure does effect the stain and delicate virus
structure. I try to use low dose exposure. Choose something off to the
side of your sample to focus on, and if necessary, do a through-focus series
to avoid creating the appearance of a substructure artifact.
If your uranyl formate or uranyl acetate is in a bottle that has sat on the
self for donkey's years, look to see that it is of uniform color and not
peppered by greenish flecks. If it is not of a uniform color, you may find
it has difficulty going into solution, indicating it time to order a new
bottle.
Hope this helps,
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
State University of New York at Binghamton
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu



From daemon Tue Aug 28 12:57:12 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Tue, 28 Aug 2001 13:55:42 -0400
Subject: Request for micrograph: cockroach cercus

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Fellow microscopists:

A colleague is looking for an electron micrograph (SEM and/or TEM) of a
cockroach cercus (or cerci) to show students in our undergraduate
physiology laboratory, who are carrying out electrophysiological
measurements with this structure. If you have a picture we could use, we
would be very grateful, and would, naturally, give you full credit.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Tue Aug 28 16:11:15 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Tue, 28 Aug 2001 17:02:46 -0400
Subject: RE: OEM service vs. Insurance Companies---Long message

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Ken,

I'll add my thanks for your viewpoint. The problem we in
Universities are having is this: you wrote
} "University service
} labs are not supposed to be profit centers. They are
} SERVICE labs and
} are heavily subsidized...."

Sadly, survey says that very few of us have administrators
who share your viewpoint. You make excellent points about
all of the options, and we can only hope this entire
discussion helps when renewal time comes around. Perhaps
the OEM Service departments need to "suit up", bring a
fancy presentation, and state their case against the ICs.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Thursday, August 23, 2001 10:53 PM, Ken Converse
[SMTP:qualityimages-at-netrax.net] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} Randy,
} Thank you. This is quite interesting.
}
} I'd like to respond from the viewpoint of one who worked
} for an OEM for
} 4 years and has subsequently had his own third party
} service company for
} 20 years.
}
} First, the OEM is obligated to service contract customers
} first. You
} have become a "billable" call and DO go to the bottom of
} the list. That
} would not be all that different with a third party firm
} (although they
} might try a little harder to be timely). The contract
} people have paid
} up front (or have at least committed up front to pay) for
} an entire
} year. This is guaranteed income and includes a
guaranteed
} liability
} (for the OEM) "to maintain the instrument/system to its
} original
} specifications". Service organizations have to take this
} seriously.
} This liability doesn't exist for billable customers.
} Work will be done
} to the best of the OEM's ability and not beyond the limit
} of the
} purchase order.
}
} Second, the IC has no technical expertise, no intimate
} connection with
} its customers and no obligation to use the manufacturer.
} I have been
} called by at least one IC several times, but have never
} done any work
} for them because they haven't a clue what I'm talking
} about when I tell
} them I service SEMs but not TEMs and I like to stay
within
} 500 miles of
} home. (Their geography hasn't impressed me, either).
} They are trying
} to emulate what happens when your car gets in a fender
} bender. There
} are lots of people who can fix it, and many of them can
do
} a pretty
} satisfactory job. But ask yourself, "When my car has a
} rumble, or the
} engine misfires and I need expert help or maintenance,
who
} do I call?
} My insurance company?" Probably not, not only because
} your insurance
} doesn't cover that kind of thing, but because they
} couldn't help you
} anyways. They know about insurance, not the inner
} workings of your car.
}
} Third, any outside party that starts making commitments
} FOR the OEM (you
} won't have to pay for recertification..............) I
} would turn away
} from and run, not walk, run. If they tell you that THEY
} will pay for
} recertification if you're unhappy and they will put it in
} writing,
} that's another story.
}
} As to the manufacturers making lots of money on their
} contracts, some
} may, some may not. A lot depends on how well they are
set
} up and run.
} It also depends on the instrument density. If one
} engineer can service
} 20 or 30 systems within driving distance, the engineer is
} competent and
} the service department backs him up well, yes, it can be
} quite
} profitable. If you have to fly to every site, you're
} short on
} experienced engineers and you don't support them well,
you
} can lose your
} shirt ( and have a whole lot of PO'd customers to boot).
}
} Every time I've gotten one of those glib calls from an IC
} in Texas, in
} the back of my mind a little voice is always saying "And
} just what makes
} you think you're going to get paid?" I do thank you for
} confirming that
} this concern is warranted.
}
} The question now comes down to "What do you want?". The
} OEM has reason
} to feel miffed. You want their expertise, their parts,
} their top level
} response, their best guess on advance parts replacement
} for reliability
} and you're going to pay someone ELSE the bonus if they do
} all this. The
} IC is betting that the instrument will run reliably as it
} always has,
} meaning very little cash out. The OEM no longer has the
} incentive to go
} out of its way to insure reliability. They'll actually
} make more if
} the system breaks more frequently. And as I pointed out,
} if they do
} their best work, you pay someone else the bonus while
they
} still have
} the considerable expense of running a service
department.
} I'm not
} implying in any way that they will go out of their way to
} make it fail.
} One of the advantages of sticking with the organization
} that actually
} does the servicing is that you establish a raport with
the
} personnel in
} the field and in house. I'm only saying that they aren't
} going to go
} out of their way to KEEP it from failing, because it's
not
} in their
} interest to do that. It IS in their interest when they
} have guaranteed
} the performance for a full year.
}
} Yes, generally speaking my service contracts are
} profitable, but there
} is another major reason that I prefer to have systems on
} contract. It
} becomes up to me how much time I spend on an instrument
} and how fast or
} slow I work on it. Sometimes I'm very sure what the
} problem is and fix
} it immediately, but often there are intermittent problems
} and I can tell
} you that a billable customer watches the clock like a
hawk
} when he sees
} me sitting in front of a running system waiting for an
} intermittent
} problem to show up. Sometimes there are subtle problems
} that the
} customer isn't even aware of, yet. If the system is on
} contract I can
} work without watching the clock and get everything right.
} In the long
} run it saves me repeat trips. Also, if I should decide
to
} let something
} slide due to whatever circumstances, I know that I'm
} guaranteeing the
} work and a later failure will be fixed at MY expense, not
} the customer's.
}
} There was a time when people seemed more likely to reward
} good service
} by paying a premium for it and showing a little loyalty
to
} those who
} provided it. The loyalty seems to be going by the board,
} and more and
} more people want more for less, they aren't willing to
pay
} for quality
} because in their everyday life they buy cheap and when it
} breaks they
} throw it out and buy cheap again. I don't believe that
} the scientific
} instrument market will get there any time soon. This is
} specialty
} equipment, capital investment, there for the long haul..
} If it's well
} taken care of , it will last for decades. That's where
} you can get the
} savings.
}
} If you were happy with the quality of the OEM's service
} and they are
} willing to work with you, by all means see what you can
} work out! If
} you weren't so happy with an OEM, see if there is a third
} party service
} company that you can establish a good relationship with.
} The ICs can't
} charge less, take out a middleman's cut, and give the
} organizations who
} actually do the work and support the equipment, enough
} money to maintain
} the quality you demand. If it looks too good to be
} true...................................................
}
} I sympathize with the cost-cutting that is being forced
on
} you from the
} bean-counters, but beware of false economies. Maybe one
} or more
} instruments could be taken off contract, but what kind of
} grants are you
} (the school and faculty) going to lose if it's down too
} much of the
} time? What are the priorities of the organization?
} University service
} labs are not supposed to be profit centers. They are
} SERVICE labs and
} are heavily subsidized because the typical student (grad
} or undergrad)
} can't afford to pay commercial rates for instrument time
} (especially
} with what they're paying for tuition). If these
} instruments are
} important to various departments then the decision is too
} important to
} be made by some MBA who doesn't know the difference
} between hydrogen
} embrittlement and cilia.
}
} It would be interesting if we could get a response from
} the insurance
} industry side of things.
}
} My $.02 worth.
}
} Sincerely,
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
}
}
} Tindall, Randy D. wrote:
}
} }
} } ----------------------------------------------------
-----
} } ---------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
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} }
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} } ml
} }
} }
---------------------------------------------------------
} } --------------.
} }
} }
} } As promised, here is a rundown on our OEM vs. insurance
} } experiences. I
} } decided to put this on the list based on the very large
} } number of replies I
} } received, some asking specifically that this be posted.
} } As a result, I've
} } removed all names, since I don't have a clue as to
legal
} } ramifications of
} } being more specific. Sorry to be so wimpy, but in these
} } cases I believe in
} } CYA.
} }
} } The following obviously reflects our experiences alone,
} } but based upon what
} } I have heard from a few others, I don't believe that
our
} } situation is
} } unique, by any means.
} }
} } Our lab, like many others, has been under increasing
} } pressure to cut costs
} } for the last two to three years, and since service
} } contracts represent a
} } significant proportion of our budget, they logically
} } became a cost-cutting
} } target immediately. It was beginning to appear that we
} } were going to lose
} } the contract on one of our TEM's to save money,
} } especially since it wasn't
} } heavily used. About this time one insurance company
} } (IC, for short) began a
} } series of high-powered presentations to the University
} } purchasing people and
} } administrators, promising significant cost reduction,
} } with no reduction in
} } services, choice of service providers, elimination of
} } paperwork, etc.
} } Facilities like ours were contacted by University
} } purchasing staff in order
} } to set up individual meetings to assess our needs and
} } get estimates on the
} } IC's costs versus OEM charges.
} }
} } After attending two open seminars and having two
} } meetings with the insurance
} } people and purchasing staff in our facility, the
} } decision was made, with my
} } agreement, to give the IC a shot at managing our
} } service. The thinking was
} } that we would try it for a year. The IC said we could
} } back out at any time
} } with no penalty, they would pro-rate our contracts to
} } adjust for the
} } differing ending dates for our OEM contracts, and, they
} } said, there should
} } be no "re-certification fee" if we decided to go back
to
} } OEM contracts,
} } since we intended to use only the OEMs' engineers
} } anyway. They would take
} } care of the paperwork notifying the OEMs about the new
} } arrangement, and all
} } billing would be handled between the IC and the service
} } providers. They
} } would guarantee no increase in contract costs for, I
} } believe, three years.
} }
} } The very first service call we made to the manufacturer
} } on one of our TEMs
} } resulted in our being told that they couldn't send an
} } engineer, because we
} } weren't on service contract any longer. When I
} } explained that they were
} } supposed to have been notified that we were with the
IC,
} } I was told that we
} } would have to provide a University P.O. before anyone
} } would be sent.
} } Apparently, there was a substantial outstanding bill
} } from this IC for
} } service provided to a university in another state, and
} } until it was paid no
} } service would be provided on our contract. The IC's
on-
} } campus rep, our
} } purchasing director, and the director of the university
} } core facilities were
} } notified by me immediately. Apparently some high level
} } phone calling went
} } on, because before the day was out, the service
provider
} } had called back
} } saying the outstanding bill would be paid and an
} } engineer was on the way.
} } (Additionally, our purchasing director assured us that
} } we could get a P.O.
} } if necessary. We have terrific support here from
} } purchasing and they have
} } been invaluable on several occasions.)
} }
} } The engineer was sent out, the scope was back on line.
} } BUT, it turns out
} } that the promised payment was never made and we were
} } back to square one the
} } next time we needed service on that machine. In fact,
} } it was worse, because
} } the OEM was never paid for the service on our scope
} } either.
} }
} } Our other scopes are from a different manufacturer and
} } initially service was
} } pretty much the same as before (although they made it
} } clear they weren't
} } happy about the switch). Response time was a bit
} } slower, and there was more
} } stinginess with replacement parts, but we had no real
} } complaints.
} }
} } Then, the IC declared bankruptcy. Their campus reps
} } disappeared, their web
} } site went down, and they effectively vanished. We had
} } already used part of
} } the year's contract, but were only able to recover
about
} } $700 on the unused
} } portion. Some labs lost thousands, I've been told.
The
} } service provider
} } was never paid, and lost a substantial amount of money.
} } (I'm told that this
} } IC has reorganized under a different name and is
} } soliciting new business,
} } especially from universities. Ask lots of questions if
} } a new company comes
} } around. Be especially careful of large numbers of
} } enthusiastic people in
} } nice suits giving slick multi-media presentations!)
} }
} } We began discussing returning to our old OEM contracts
} } and I was asked to
} } get prices. The first thing I was told by one service
} } provider was that we
} } would have to have our instrument re-certified, which
} } would cost about
} } $1500, even though nobody had touched that scope but
the
} } OEM engineers.
} } Needless to say, that rankled a bit, so we checked into
} } another insurance
} } company that had been recommended by several other
labs.
} } We were
} } immediately impressed by the fact that they presented
} } what seemed to be a
} } more realistic picture of what they could do for us.
} } They also offered
} } significant savings (10-15%) over the old contracts, so
} } we once again
} } decided to give it a try.
} }
} } This time, the first call we made had the same result
as
} } before, i.e., the
} } provider wanted a P.O. before an engineer would come
} } out. When I asked why,
} } they said that even though they had no problems with
the
} } new IC, they
} } weren't going to take a chance on losing a ton of money
} } again. If the
} } university would sign a waiver accepting responsibility
} } for paying any
} } amounts in default, we could get service again. Fine,
} } I said. Several
} } days later the form was faxed to us and I forwarded it
} } to our purchasing
} } director, who responded that he had passed it along for
} } review to the people
} } who needed to sign off on it. That was last month
} } sometime, and I have
} } heard nothing yet. So far we have been waiting to get
a
} } preventive
} } maintenance visit on this scope for about two months
and
} } nothing's in sight,
} } yet.
} }
} } On our other scopes, we have again been able to get
} } service, but now it
} } seems that we are way, way down on the priority list.
} } OEMs will tell you
} } that they are obligated to service their contract
} } customers first, and in
} } our experience they certainly do. The service
} } management (insurance)
} } companies will tell you that this is not the case, that
} } it's first-come,
} } first-served. That has not been our experience. On
one
} } TEM we have been
} } waiting weeks for a PM. It was scheduled twice and an
} } engineer was here,
} } but was pulled away on "emergency" calls, which I read
} } as calls from
} } contract holders. The PM has not yet been done. (Just
} } minutes ago we
} } received a call from our service engineer saying he's
} } been pre-empted again
} } and now he has NO idea when he can get here.) Our SEM
} } has been serviced
} } under the new arrangement in a relatively timely
manner.
} }
} }
} } Under the OEM contracts, service was provided
} } "yesterday" and parts were
} } replaced if they were even suspected to be bad. The
} } working relationship
} } was wonderful, and the service was stellar. We were
} } called and reminded of
} } PMs before they were due and we received periodic phone
} } calls just to check
} } on the status of our equipment. Under the new
} } contracts, service is
} } provided (if we can get it) whenever they get around to
} } it, and engineers
} } can be interrupted by calls to respond to contract
} } holders. Parts are
} } changed less frequently and are all billed. We
schedule
} } the PMs (no big
} } deal). The relationship is much more tense than it
used
} } to be.
} }
} } We could probably increase the level of service by
} } reminding the service
} } people that when it's time to replace our scopes,
} } service history will have
} } a MAJOR bearing on whose machines we buy. However,
I'm
} } extremely reluctant
} } to turn a previously very pleasant relationship into a
} } confrontational,
} } grudging one (although it seems to be headed that way,
} } anyway). In
} } addition, I can understand why some of these things are
} } happening. If I put
} } myself in the shoes of the OEM service managers, I'm
not
} } sure what I would
} } do differently. On the other hand, I also have a tiny,
} } nagging feeling that
} } part of these problems are deliberately designed to
} } pressure us back into
} } our old contracts. That possibility (and I don't know
} } it to be true) annoys
} } me intensely, but I still haven't played the sales
} } managers against service
} } managers card. My sense is that OEMs make big bucks on
} } service contracts
} } and they take it VERY personally when we "fire them" as
} } one service manager
} } phrased it.
} }
} } I would like to emphasize very strongly that when field
} } service engineers
} } come out, they are uniformly excellent. They do
} } everything they are allowed
} } to do to provide top-notch care for the instruments.
} } The problems
} } originate higher up.
} }
} } In summary, we have found that service management
} } companies do indeed save
} } money on comparable contracts, but we have had a
} } miserable time getting
} } service at all when using them. This may not be
} } typical---I don't know.
} } Our level of service has declined greatly in terms of
} } promptness, attitude,
} } and parts supply. The attitude part was certainly not
} } worth the extra
} } several thousand dollars the OEMs wanted, but the
} } promptness and parts
} } issues probably were. Most disturbing was finding out
} } that we could be
} } denied service because of a problem originating at
} } another university in
} } another state that had absolutely nothing to do with
us.
} }
} }
} } We will continue for now with our new IC's to see if
the
} } problems correct
} } themselves, especially since the problems didn't arise
} } with this company.
} } In addition, one of the OEM service managers called and
} } suggested we might
} } try a custom contract in which we could work with them
} } to design our own
} } service schedule. He said we could be informally
} } trained during service
} } visits to perform many of the maintenance chores on the
} } scope and could
} } retain a contract that would basically cover
} } emergencies, at a substantial
} } cost savings. We are looking into this now, because it
} } seems to have lots
} } of nice possibilities. (We already do filament/gun
} } exchanges and cleanings,
} } obviously, but I've never personally done a mechanical
} } column alignment or
} } routine maintenance requiring major disassembly.)
} }
} } My best advice: if you are currently with an OEM, stay
} } there. If you are
} } losing your local in-house service wizard and need to
} } decide on contracts,
} } go with the OEMs. Fight like hell to resist pressure
to
} } switch.
} }
} } If you are with an insurance company and are happy with
} } them, stay there,
} } and I wish you continued good luck. Please tell me
your
} } secret.
} }
} } My suggestion to OEM service providers: offer some in-
} } house maintenance
} } training courses, better maintenance manuals and
} } schematics, and encourage
} } customers to do more maintenance tasks (with
appropriate
} } provisos for
} } covering dumb mistakes). Lower your contract prices so
} } we're not so
} } pressured to drop them. The glory days are over,
folks.
} } Time to compete.
} }
} } As for me, I have no financial interest in any of these
} } folks. I just want
} } our scopes to work. Feel free to contact me with any
} } questions.
} }
} }
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
} }
} }
} }
} }
} }



From daemon Tue Aug 28 17:32:05 2001



From: Jeannette Taylor :      jvtaylo-at-emory.edu
Date: Tue, 28 Aug 2001 17:26:27 -0500
Subject: query methyl amine vanadate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate information on methyl amine vanadate and where to buy
it. It is used as a negative stain.

Thank you,
Jeannette Taylor


From daemon Tue Aug 28 19:48:57 2001



From: Aurelie Snyder :      snyderau-at-ohsu.edu
Date: Tue, 28 Aug 2001 17:40:51 -0700
Subject: About digitalized video microscope

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,
I need your suggestions about the possiblity to buy a system based on
the inverted microscope, but suitable for deconvolution and
time-lapse analysis of fast events in living cells.

We would like to buy such system. Whinch one is the best (I mean
quality devided on price)?

Sincerely yours, Alexander Mironov
Consorzio Mario Negri Sud, Italy




From daemon Tue Aug 28 22:54:27 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 28 Aug 2001 20:47:11 -0700
Subject: Re: query methyl amine vanadate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try NanoProbe Inc
Sergey

At 05:26 PM 8/28/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Aug 29 01:29:41 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Wed, 29 Aug 2001 02:20:18 -0400
Subject: Re: query methyl amine vanadate - commercial vendor reply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Jeannette:

We (Nanoprobes) sell methylamine vanadate as a 2 % aqueous solution
(suitable for use directly) - the product is called "NanoVan." It's listed
on our web site catalog under "Negative staining."

Regards,

Rick Powell

*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Wed Aug 29 07:16:54 2001



From: Louise Taylor :      louiset-at-mail.saimr.wits.ac.za
Date: Wed, 29 Aug 2001 14:08:33 +0200
Subject: RE: DNA Extraction

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Has anybody out there successfully extracted DNA from Resin embedded EM
tissue. If yes, what are your methods, protocols?

Thanks in advance

Ulrike Allard

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa





From daemon Wed Aug 29 08:15:03 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 29 Aug 2001 09:06:16 -0400
Subject: RE: query methyl amine vanadate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try:
Joseph S. Wall, Ph.D. 516-344-2912; Fax: 516-344-3407 E-mail: wall-at-stem.bio.bnl.gov
Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/

} ----------
} From: Jeannette Taylor
} Sent: Tuesday, August 28, 2001 6:26 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: query methyl amine vanadate
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate information on methyl amine vanadate and where to buy
} it. It is used as a negative stain.
}
} Thank you,
} Jeannette Taylor
}
}


From daemon Wed Aug 29 08:33:01 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 29 Aug 2001 09:26:46 -0400
Subject: RE: query methyl amine vanadate-2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Finally got it right.

Nanoprobes, Inc.

http://www.nanoprobes.com/Nstain2.html

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Jeannette Taylor
} Sent: Tuesday, August 28, 2001 6:26 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: query methyl amine vanadate
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate information on methyl amine vanadate and where to buy
} it. It is used as a negative stain.
}
} Thank you,
} Jeannette Taylor
}
}


From daemon Wed Aug 29 09:56:54 2001



From: Niko Grigorieff :      niko-at-brandeis.edu
Date: Wed, 29 Aug 2001 10:48:35 -0400
Subject: TEM post-doc position available

Contents Retrieved from Microscopy Listserver Archives
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Post-doctoral position at Brandeis University

A post-doctoral position is immediately available to work on the development
of new computational methods in image processing. The candidate will join a
multidisciplinary team working in the field of structural biology. Our goal is
to determine the three-dimensional structures of macromolecular complexes using
high-resolution electron cryo-microscopy. Electron microscopy is a rapidly
developing technique to explore a large number of cellular structures with
medical and biological importance. Our laboratory has a well-established
electron microscope facility, and equipment for protein purification, sample
preparation, and image processing. Image data are currently being generated by
several projects in our laboratory. Processing is performed on our UNIX alpha
and SGI workstations using software partly developed in our laboratory. The
role of the post-doctoral researcher will be to improve existing, and develop
new image processing algorithms to push protein structure determination by
electron microscopy towards atomic resolution. One important objective will be
to develop a detailed understanding of electron scattering from biological
samples, and to develop algorithms to extract information from electron
scattering patterns and images.

The ideal candidate would have a background in applied physics or material
sciences. Candidates from other fields with experience in microscopy, as well
as light, electron or X-ray scattering techniques are also encouraged to apply.

Formal applications, which should include a CV, statement of research
interests, and the names, addresses and e-mails of 3 references, should
be sent to

Dr. Nikolaus Grigorieff
Brandeis University - MS029
415 South Street
Waltham, MA02454-9110
USA

Tel: +1-781-736-2444
Fax: +1-781-736-2419
Email: niko-at-brandeis.edu

Brandeis University is an Equal Opportunity Employer.





From daemon Wed Aug 29 12:26:24 2001



From: Jeff Hedlesky :      jhedlesky-at-edgebb.net
Date: Wed, 29 Aug 2001 12:12:12 -0500
Subject: LM nucleated CaCO3 polymorphs and algae eutrophication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopy Wisemen (and women!):

I hope you will indulge me with a moment of your time.

I'm involved with a group here in Wichita that's working on some water
treatment technologies that reduce chemical usage as compared to current

treatment methodologies. We also are working on a system using a
version of these same new technologies to reduce algae growth in pond
water. I've been conscripted to help them assemble the "water lab" for
experimentation and testing and generally manage the development
project.

Well, I've done plenty of development projects, but my knowledge of
microscopy is pretty much limited to the Skillcraft 'scope I had as a
child and the low magnification bench scopes I've set up for
microelectronics manufacturing. I am absolutely delighted to have found

your microscopy.com group and have been "cramming" for the past
week or so. I think I'm now ready to ask you a couple of questions.
(and maybe make sense...)

The two main (and quite different) thrusts of microscopic analysis that
we're attacking first are determining the polymorphic state that calcium

carbonate is present in, in potable water (aragonite or calcite) and the

viability of algae that has been exposed to "treated" water in a pond
setting.
What I'm most curious about is your (expert) opinion as to the best
strategies to image these two areas of interest, and to do so out to a
high-
quality digital imaging system. (Nikon, SPOT RT, etc.)

After nucleation, the calcium carbonate in question will either be in
colloidal suspension in liquid water or will be dried onto a slide. I'm
betting
that a water immersion objective would be your recommendation for the
liquid viewing, but I'd rather hear it from you. Sure, if we had an
electron
microscope, we could image individual seed crystals, BUT a) we don't
have
that kind of budget and b) we're pretty sure we can induce nucleation
and
crystal growth on demand, so we'll just grow 'em until we can see 'em.
I
guess what I'm more curious about is whether you feel that good old
fashioned transmissive light microscopy is the right path or whether
we'd see
significant advantage in exploring polarizing light, phase contrast, DIC

or some other kinky form of imaging to best see the orthorhombic nature
of the calcite or the hexagonal prismatic aragonite. For what it's
worth, I've read somewhere of using a special setup to actually derive
the
refractive index of the sample in question. Aragonite does have a
different
value in this respect than does calcite - in fact aragonite's value is
higher
than the two values for calcite (birefringent?). Eeek! I now know
just enough to hurt myself and not much more...

As for the algae, we're trying to show that we have a process for
killing it, so primarily we're looking at ways to image healthy
(pre-treatment) specimens and then treat the water and show (over time)
that we really do kill the stuff. Again, same question - is there some
creative way to do this with good 'ol planapochromatics or do I need a
pricey Fluorescence setup to make the chlorophyll twinkle? OR "What
exactly does freshly dead or dying algae look like and how do you best
look at it?".

We'll be doing some particle sizing and Zeta potential analysis as well,

so we're not trying to be cheap on the microscopy side - BUT, I don't
want to buy more or less than we need and the 'scope salesmen all think
we need everything they sell...

Any light you have time to shed on these issues will be greatly
appreciated - if there's a chance of speaking with you on these issues
or on the possibility that either you or someone you respect being
available for some consulting work in these areas, please let me know
and I'll
gladly set up a conference call. Much gratitude for whatever you can
do!

Best regards,

Jeff
--
Jeff Hedlesky
Aladdin Companies
Wichita, KS




From daemon Wed Aug 29 14:37:57 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Wed, 29 Aug 2001 14:30:10 -0500
Subject: in situ hybridization

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
I'm hoping that someone out there thinks that in situ localization using
non-radioactive DIG-labelled RNA probes on sections of tissue on glass
slides is almost as easy as flipping a light switch. I would greatly
appreciate some advice as I've been banging my head against the wall for
months trying to get this to work.
The background is that I'm doing the in situ work with plant tissue, namely
roots that have been lightly fixed in a paraformaldehyde/glut. mixture (as
per in situ chapter in Steven Ruzin's bible of plant microtechnique) and
embedded in butyl-methyl-methacrylate as per Tobias Baskin's methods
(thanks again Tobias). After some struggle with the molecular biology side
of the experiment, I found that the gene I was given to work from had been
subcloned along with a bit of "junk" DNA from the old plasmid it had been
in. I used PCR to amplify ~100 bp pieces of that gene to get around the
"junk" and to avoid having to hydrolyse my probes, since I often lose a
large amount of probe when I hydrolyse.
The questions I have relate more to the detection side of the matter. I've
been using about 4ng of RNA probe per slide (as recommended in the Ruzin
bible), then using an anti-DIG (Fab fragments) antibody conjugated to
alkaline phosphatase supplied by Roche. Then I "develop" the labelling
using the standard NBT-BCIP detection used often-times for Western blots.
The controls work pretty well at this point.
My main question is, has anyone used fluorescently conjugated anti-DIG
antibodies for this type of work (or a secondary fluorescent conjugate)? It
seems that it would be much more straight-forward than guessing when the
NBT-BCIP reaction has reached a good level of staining.
Any other insights/advice would be appreciated.
Thanks again for your help,
Kristen

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011

515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Wed Aug 29 18:01:01 2001



From: MELISSA ANN LEWIS :      lewisma-at-medicine.ufl.edu
Date: Wed, 29 Aug 2001 17:54:04 -0500
Subject: Cryo sectioning

Contents Retrieved from Microscopy Listserver Archives
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I am trying to section with a cryo diamond knife that has a boat. I
am not sure on what solution to use in the boat. So far we have tried a
DMSO and ETOH, 50/50 mixture. This slowly froze over. Please help:)
Melissa


From daemon Wed Aug 29 18:01:01 2001



From: kai-at-lehigh.edu ()
Date: Wed, 29 Aug 2001 17:57:20 -0500
Subject: Ask-A-Microscopist: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kai-at-lehigh.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 29, 2001 at 15:34:48
---------------------------------------------------------------------------

Email: kai-at-lehigh.edu
Name: Kai Lorcharoensery

Organization: Lehigh University

Education: Graduate College

Location: Bethlehem, PA, USA

Question: TEM sample preparation: I would like to see the structure
of Fe particle cross-section. These particles are about
40-100 um in size. I tried casting them with epoxy in
3-mm copper tube but either particles fell off or
epoxy thin film (100 um) was detached from Cu ring.

The preparation steps are
1. Casting
2. Slicing the Cu tube with high speed diamond saw
3. Grinding with 600 grit- 8 um- 3 um SiC paper
4. Polishing with 1 um Al2O3 on nylon cloth
==} ~ 80-100 um and here is where the epoxy disk was
usually detached from the Cu ring.

Could you please suggest some tips?

Thank you

---------------------------------------------------------------------------


From daemon Wed Aug 29 18:48:14 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Wed, 29 Aug 2001 16:39:43 -0700
Subject: Post-doctoral Position

Contents Retrieved from Microscopy Listserver Archives
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Position Announcement
Chemical and Materials Engineering Dept.
Arizona State University

A post-doctoral candidate is sought to work on an interdisciplinary
research project to study the interaction of functional molecular
monolayers with nanostructured arrays on electronic materials. A new
interface force microscope will be constructed and studies conducted on
molecular monolayers on quantum dots, wires, etc. Emphasis will be placed
on biomimitic molecules containing functional and transductional components
with potential for electronic applications. Position available
immediately. Please send cover letter and resume including the names,
addresses and phone or e-mail of 3 professional references to
picraux-at-asu.edu {mailto:picraux-at-asu.edu} . Preference will be given to
applications received by September 14, 2001 and will be continued until the
position is filled.


Dr. S. Thomas Picraux
Arizona State University
Dept. of Chemical and Materials Engineering
Box 876006
Tempe, AZ 85287-6006


John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Wed Aug 29 20:39:37 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 29 Aug 2001 21:35:43 -0400
Subject: Re: LM nucleated CaCO3 polymorphs and algae eutrophication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,

Re: the carbonate morphologies

Perhaps with controlled nucleation and experience you could induce
recognizable and differentiable calcite and aragonite forms to grow.
However, these materials can take on a wide variety of crystal habits
depending upon the other ionic species and concentrations present,
temperature and organic materials present. Often acicular spherical
crystal groups will form and the individual crystals will not be
distinguishable with sufficient clarity to determine their optical
properties. It is also possible to arrive at situations where mixed
phase agglomerates form.

Calcium carbonate is subject to the influence of organic materials which
strongly influence crystal morphology by preferentially binding to one
or another of the crystal faces. In this way the aspect ratio of the
crystals may change dramatically from one batch to another if trace
organics vary. Other organics may influence the degree of saturation of
the solution with respect to calcium carbonate. For example, most sea
water is undersaturated with respect to calcium carbonate even though
vast amounts of limestone occur in contact with it. One reason is that
a very small amount of free fatty acid is sufficient to complex with the
exterior of the carbonate phase, in effect isolating the solid surface
and interrupting the solid {==} solution equilibrium.

To get an idea of how varied the crystal habit of this material can be
(and to gain an appreciation for living in the age of X-ray
diffraction!) you might take a look at the classic work on morphological
description by Victor Goldschmidt, "Atlas der Krystallformen". There is
an entire volume out of the library-shelf-size "Atlas" on calcite.

Good luck.

John Twilley
Conservation Scientist

Jeff Hedlesky wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopy Wisemen (and women!):
}
} I hope you will indulge me with a moment of your time.
}
} I'm involved with a group here in Wichita that's working on some water
} treatment technologies that reduce chemical usage as compared to current
}
} treatment methodologies. We also are working on a system using a
} version of these same new technologies to reduce algae growth in pond
} water. I've been conscripted to help them assemble the "water lab" for
} experimentation and testing and generally manage the development
} project.
}
} Well, I've done plenty of development projects, but my knowledge of
} microscopy is pretty much limited to the Skillcraft 'scope I had as a
} child and the low magnification bench scopes I've set up for
} microelectronics manufacturing. I am absolutely delighted to have found
}
} your microscopy.com group and have been "cramming" for the past
} week or so. I think I'm now ready to ask you a couple of questions.
} (and maybe make sense...)
}
} The two main (and quite different) thrusts of microscopic analysis that
} we're attacking first are determining the polymorphic state that calcium
}
} carbonate is present in, in potable water (aragonite or calcite) and the
}
} viability of algae that has been exposed to "treated" water in a pond
} setting.
} What I'm most curious about is your (expert) opinion as to the best
} strategies to image these two areas of interest, and to do so out to a
} high-
} quality digital imaging system. (Nikon, SPOT RT, etc.)
}
} After nucleation, the calcium carbonate in question will either be in
} colloidal suspension in liquid water or will be dried onto a slide. I'm
} betting
} that a water immersion objective would be your recommendation for the
} liquid viewing, but I'd rather hear it from you. Sure, if we had an
} electron
} microscope, we could image individual seed crystals, BUT a) we don't
} have
} that kind of budget and b) we're pretty sure we can induce nucleation
} and
} crystal growth on demand, so we'll just grow 'em until we can see 'em.
} I
} guess what I'm more curious about is whether you feel that good old
} fashioned transmissive light microscopy is the right path or whether
} we'd see
} significant advantage in exploring polarizing light, phase contrast, DIC
}
} or some other kinky form of imaging to best see the orthorhombic nature
} of the calcite or the hexagonal prismatic aragonite. For what it's
} worth, I've read somewhere of using a special setup to actually derive
} the
} refractive index of the sample in question. Aragonite does have a
} different
} value in this respect than does calcite - in fact aragonite's value is
} higher
} than the two values for calcite (birefringent?). Eeek! I now know
} just enough to hurt myself and not much more...
}
} As for the algae, we're trying to show that we have a process for
} killing it, so primarily we're looking at ways to image healthy
} (pre-treatment) specimens and then treat the water and show (over time)
} that we really do kill the stuff. Again, same question - is there some
} creative way to do this with good 'ol planapochromatics or do I need a
} pricey Fluorescence setup to make the chlorophyll twinkle? OR "What
} exactly does freshly dead or dying algae look like and how do you best
} look at it?".
}
} We'll be doing some particle sizing and Zeta potential analysis as well,
}
} so we're not trying to be cheap on the microscopy side - BUT, I don't
} want to buy more or less than we need and the 'scope salesmen all think
} we need everything they sell...
}
} Any light you have time to shed on these issues will be greatly
} appreciated - if there's a chance of speaking with you on these issues
} or on the possibility that either you or someone you respect being
} available for some consulting work in these areas, please let me know
} and I'll
} gladly set up a conference call. Much gratitude for whatever you can
} do!
}
} Best regards,
}
} Jeff
} --
} Jeff Hedlesky
} Aladdin Companies
} Wichita, KS
}
}
}
}
}



From daemon Thu Aug 30 05:01:52 2001



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Thu, 30 Aug 2001 11:54:52 +0200
Subject: Microscopy resources have moved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Let me inform you that my so called "Petr's Microscopy Resources"
have been moved to the new address

http://www.petr.isibrno.cz/microscopy/

The items Societies and Meetings are rebuilt. The other items
(Literature, Database, Laboratories, Production & Sales, Courses &
Software, Information Resources) have been modified, and will be
transferred to the new technology too.

Regards,

Petr Schauer
+---------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+---------------------------------------------------------------------+


From daemon Thu Aug 30 06:31:10 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 30 Aug 2001 07:43:18 -0400 (EDT)
Subject: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff,

I don't know anything about algae but there are other ways to solve your CaCO3 question. You are not
interested in the shapes or sizes of the particles, right ? So IR or Raman spectroscopy might be the
way to go, these are two references on that topic: Calcium carbonate phase analysis using XRD and
FT-Raman spectroscopy. Kontoyannis, Christos G.; Vagenas, Nikos V. Inst. Chem. Eng. High Temperature
Chem. Processes, Dep. Pharm., FORTH, University of Patras, Patras, Greece. Analyst (Cambridge, U.
K.) (2000), 125(2), 251-255. Simpson, L. J. Electrochemically generated CaCO3 deposits on iron
studied with FTIR and Raman spectroscopy. Electrochim. Acta (1998), 43(16-17), 2543-2547. There
are other references, but that should do for a start. There is also a fancy tool called IR or Raman
microscope. This thing combines optical microscopy and optical spectrometer. Therefore you can take
Raman or IR spectra from individual particles and see which ones are vaterite or aragonite or
whatever phase you are looking for. I've done that with ZnO particles about 500 nm to 1 micron in
size, so I actually know it works.

Hope this helps
Andreas

*************************************************
Dr. Andreas Taubert
Dept. of Materials Science and Engineering
3231 Walnut Street
University of Pennsylvania
Philadelphia PA 19104-6272
tel: +1 215 898 2700
fax: +1 215 573 2128

Physical Chemistry is everything for
which 1/T is linear ...
*************************************************

----- Original Message -----
} From: Jeff Hedlesky {jhedlesky-at-edgebb.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 29, 2001 1:12 PM


Hi Listers...

Does anyone know when the first cold field emission microscope became
commercially available?

Thanks and best,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977




From daemon Thu Aug 30 07:46:41 2001



From: Don.Steele-at-alcan.com
Date: Thu, 30 Aug 2001 08:40:04 -0400
Subject: Re: Ask-A-Microscopist: TEM sample preparation: Particle sectioning

Contents Retrieved from Microscopy Listserver Archives
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You might try either

1) A "coupling agent" to promote adhesion of the copper to the epoxy. I
have used Dow Z-640 (originally meant as a coupling agent for fibers in
fiber glass, I believe), to reduce pullout of particulates during
microtomy. This was originally suggested by Klinger and Schwab (I can look
up the full reference if you are interested).

2) Incorporating the particulate into a metal foil instead of an epoxy.
This involves either electroless nickel plating or electroplating of a
sufficiently thick film that it can then be handled as a "bulk" sample.
Either give good adhesion to the particles (at least in the case of the
aluminum powders or ceramic particles with which I am familiar).
Electroless plating has the benefit of bonding to non conductive materials.
You might look at Morra et al (Materials Sci. and Eng. A124, 1990, "A
Technique for prep. of powders...") as a reference.



kai-at-lehigh.ed
u () To: Microscopy-at-sparc5.microscopy.com
cc:
08/29/01 Subject: Ask-A-Microscopist: TEM sample preparation
06:57 PM






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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kai-at-lehigh.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 29, 2001 at 15:34:48
---------------------------------------------------------------------------

Email: kai-at-lehigh.edu
Name: Kai Lorcharoensery

Organization: Lehigh University

Education: Graduate College

Location: Bethlehem, PA, USA

Question: TEM sample preparation: I would like to see the structure
of Fe particle cross-section. These particles are about
40-100 um in size. I tried casting them with epoxy in
3-mm copper tube but either particles fell off or
epoxy thin film (100 um) was detached from Cu ring.

The preparation steps are
1. Casting
2. Slicing the Cu tube with high speed diamond saw
3. Grinding with 600 grit- 8 um- 3 um SiC paper
4. Polishing with 1 um Al2O3 on nylon cloth
==} ~ 80-100 um and here is where the epoxy disk was
usually detached from the Cu ring.

Could you please suggest some tips?

Thank you

---------------------------------------------------------------------------






From daemon Thu Aug 30 07:59:29 2001



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 30 Aug 2001 07:55:45 -0500
Subject: Welcome to the Microscopy Listserver Cc:

Contents Retrieved from Microscopy Listserver Archives
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*********************************************
This Email contains Important Information about
the Microscopy Listserver. Please read it all then
SAVE a copy for future reference. - Nestor
****************************************


From daemon Thu Aug 30 08:09:16 2001



From: zaluzec-at-microscopy.com
Date: Thu, 30 Aug 2001 08:05:20 -0500
Subject: Administrivia: Oops...Nestor slipped

Contents Retrieved from Microscopy Listserver Archives
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Sorry Colleagues...

The message you all received was a blunder.. too many hours on-line
and my fingers and mouse start wandering aimlessly missing the correct
keys. It was supposed to go out to today's set of Newsubscribers only, not the
entire list.

Nestor
Your Friendly Neighborhood SysOp


From daemon Thu Aug 30 08:33:32 2001



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Thu, 30 Aug 2001 09:22:03 -0400
Subject: Fwd: Cryo sectioning

Contents Retrieved from Microscopy Listserver Archives
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} Melissa: It depends on what temperature you are using when
} sectioning. When I am sectioning a temperatures that freeze the DMSO mix
} I use ethanol. Ethanol will stay a liquid down to about -95C. With our
} microtome you can control temp of knife and boat and gas separately so I
} can keep the knife temp to about -90C and can still have the sample a
} little lower than -120C. This enables me to section siloxanes. If you
} need to go colder than this I think you will have to try and section
} dry. Hope this helps. Steve
}
} I am trying to section with a cryo diamond knife that has a boat. I
} am not sure on what solution to use in the boat. So far we have tried a
} DMSO and ETOH, 50/50 mixture. This slowly froze over. Please help:)
} Melissa

Stephen McCartney
Research Associate
2108 Hahn Hall
Materials Institute
Virginia Tech
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Thu Aug 30 09:36:18 2001



From: Greg Strout :      gstrout-at-ou.edu
Date: Thu, 30 Aug 2001 09:30:40 -0500
Subject: Re: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking at an advertisement from Coats and Welter Instruments for "The
world's first commercial field emission scanning electron microscope." It
is touted as ...operating at room temperature and not being subject to
burnout as are the conventional hot filament electron emitters...
While the ad is not dated it does reference a paper from 1968 so it was
probably produced sometime in the late 60's or very early 70's.
Greg

Angela Klaus wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers...
}
} Does anyone know when the first cold field emission microscope became
} commercially available?
}
} Thanks and best,
}
} Angela
}
} Angela V. Klaus
}
} Director, Interdepartmental Laboratories
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Thu Aug 30 10:31:09 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 30 Aug 2001 11:25:13 -0400
Subject: RE: OEM service vs. Insurance Companies---Long message

Contents Retrieved from Microscopy Listserver Archives
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Ken,

I think we would ALL love to see tuition supporting
inter-departmental facilities. Departments are not profit
centers per se, but may be expected to be self-sufficient.
And from what (little) I'm learning about administrative
thought processes...your childrens' tuition is essentially
"credited" to the departments (or school) in which they
major. Maybe not directly as hard dollars, but in terms of
"weight" to be thrown around :) Inter-departmental,
multi-user facility, interdisciplinary....great buzzwords
but no real money.

My $0.02...based on broad impressions of OTHERS'
experiences. (I don't yet know whether I am describing the
views of my employer....)

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
mlynn-at-miami.edu


On Wednesday, August 29, 2001 9:09 PM, Ken Converse
[SMTP:qualityimages-at-netrax.net] wrote:
} Matt,
} I'll repeat what I said to Phil Oshel:
} If I found a school turning any profit on a service lab,
} I'd scream
} bloody murder as I've got 2 in college and 2 on the way.
} I would expect
} my tuition bills to go a long way towards general
support.
}
}
} My take as a parent.
}
} Ken Converse
} owner (and parent of 4)
} Quality Images
} third party SEM service
} Delta, PA
}
}
} Matthew Lynn wrote:
}
} }
} }
---------------------------------------------------------
} } ---------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} }
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} } ml
} }
} }
---------------------------------------------------------
} } --------------.
} }
} }
} } Ken,
} }
} } I'll add my thanks for your viewpoint. The problem we
} } in
} } Universities are having is this: you wrote
} }
} } } "University service
} } } labs are not supposed to be profit centers. They are
} } } SERVICE labs and
} } } are heavily subsidized...."
} }
} }
} } Sadly, survey says that very few of us have
} } administrators
} } who share your viewpoint. You make excellent points
} } about
} } all of the options, and we can only hope this entire
} } discussion helps when renewal time comes around.
} } Perhaps
} } the OEM Service departments need to "suit up", bring a
} } fancy presentation, and state their case against the
} } ICs.
} }
} } Matt
} }
} } Matthew J. Lynn
} } Center for Advanced Microscopy
} } University of Miami
} } (305)284-4736
} } mlynn-at-miami.edu
} }
} }



From daemon Thu Aug 30 10:58:45 2001



From: Yoho, Tim :      TYoho-at-lhup.edu
Date: Thu, 30 Aug 2001 11:52:13 -0400
Subject: Wanted: Used SEM

Contents Retrieved from Microscopy Listserver Archives
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Since our JSM 25 died, we are looking for a good used SEM preferably in
the following models:

1. Hitachi S570

2. JOEL no older than 10 years

3. Philips no older than 10 years

Please contact me at: tyoho-at-lhup.edu

---------------------------------------------------------
Tim P. Yoho, Professor Yoho (Joho) Amateur Radio:
WA3D
Department of Biology First Known Use in 1395 (570)
893-2391
Lock Haven University Origin: Switzerland & Alsace Fax: (570)
893-2047
Lock Haven, PA. 17745 http://www.lhup.edu/~tyoho tyoho-at-lhup.edu
---------------------------------------------------------


From daemon Thu Aug 30 11:08:57 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 30 Aug 2001 09:01:53 -0700
Subject: Re: Ask-A-Microscopist: TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Kai,
When doing samples like you have described, I usually grind to 100 microns
using a Disc Grinder from Gatan, or the same device from other specimen prep
companies, on 1200 grit paper stuck on a sheet of glass. If detachment is
your problem, then cast your sample/epoxy into a slightly larger copper tube
with INSIDE diameter of 3 mm. and glue a large-mesh grid or slot grid onto
the exposed end of your sample when it is still fairly thick. Then
detachment is desirable and will leave your sample stuck onto a grid for
final thinning, dimple polishing, ion thinning or whatever you wish to
attain transparency.
At 05:57 PM 8/29/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Aug 30 12:43:56 2001



From: Rik Brydson :      mtlrmdb-at-leeds.ac.uk
Date: Thu, 30 Aug 2001 18:22:28 +0100
Subject: FW: MICROSCIENCE 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




MICROSCIENCE 2002 London UK JULY 9-11 2002

An international conference and exhibition on the science of microscopy and
in-situ analysis; a unique opportunity for you to hear about topics at the
cutting edge of microscopy and to see for yourself the very latest
developments in light, scanning probe and electron microscopes, associated
equipment and image analysis systems in both life and physical sciences.



The Science...

The conference programme will be a series of Invited Lectures by
internationally acclaimed experts, which will be closely linked to
Tutorials, Poster Sessions and special "hands-on" Workshops.

Topics include:

fluoresence & molecular markers
confocal & multiphoton microscopy
FLIM & FRET
low-temperature microscopy
variable pressure SEM
tomography
digital image acquisition and analysis
scanning probe microscopy
energy filtered imaging
EBSD
FEGTEM
SuperSTEM


The Exhibition.....

MICROSCIENCE 2002
will host the UK¹s premier exhibition
of light, electron and scanning probe microscopes and associated equipment.
See the latest developments in this rapidly developing field.


For more information, contact the RMS by email

Info-at-rms.org.uk

Or visit the website

www.rms.org.uk/microscience2002


--
_____________________________
Dr. Rik Brydson,
Leeds Electron Microscopy and Spectroscopy (LEMAS) Centre
Department of Materials,
School of Process, Environmental and Materials Engineering
University of Leeds,
Leeds LS2 9JT,
U.K.

Tel: 44 + (0)113 233 2369
Fax: 44 + (0)113 242 2531
Web: http://www.materials.leeds.ac.uk
Web: http://www.materials.leeds.ac.uk/lemas

______________________________

**************************
Join email discussion list for all aspects of
Electron Microscopy and Analytical Spectroscopy (LEMAS)

visit website
http://www.jiscmail.ac.uk/lists/lemas.html
and follow instructions
**************************

=====================================
EELS and X ray database : http://www.cemes.fr/~eelsdb/
=====================================


--


From daemon Thu Aug 30 13:47:53 2001



From: Hanika, William :      haniwc-at-stlo.smhs.com
Date: Thu, 30 Aug 2001 13:40:52 -0500
Subject: MT 1B Maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm having a problem with my MT-1B Ultra microtome. When cutting ultra thin
sections my microtome skips cutting a section completely then cuts a section
that's too thick. Any solutions? I can't find a company that services
Sorvall MT-1B microtomes. Anyone have a source for service calls.
Bill


From daemon Thu Aug 30 14:44:38 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 30 Aug 2001 10:04:11 -1000 (HST)
Subject: Re: MT 1B Maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeff -

Fourier transform infrared microspectroscopy (a.k.a. infrared microscopy)
may prove more useful to your work than polarized light microscopy to
differentiate and identify polymorphs of calcium carbonate. The former
provides chemical analysis of molecular composition, while the latter
provides examination of morphology and measurement of optical properties.

Infrared microscopy couples an optical microscope to an FTIR spectrometer.
The optical microscope is used to position small areas of a specimen in a
condensed infrared beam for analysis. The FTIR spectrometer gives an
infrared spectrum that is used to identify molecular composition and to make
qualitative and quantitative analyses of samples. The technique can
distinguish between calcium carbonate polymorphs such as calcite and
aragonite.

Most infrared microscopes are equipped for brightfield illumination and
transmitted visible illumination. Most systems, like mine, also can be
fitted for polarized light examination and brightfield fluorescence
illumination, which can be useful in differentiating phases in specimens.
Analyses can be made in transmission if the specimen is prepared on an
infrared-transparent window material such as diamond, KBr, BaF2, etc., or by
reflection-absorption if dispersed on a reflective surface such as a gold
filter. Other methods are used, but may limit your ability to differentiate
particles by morphology before analyses. Preliminary tests will help you
develop a methodology.

If your specimen particles are individual and smaller than 5-10 micrometers
in diameter, then you might want to investigate Raman microscopy, which
complements infrared microscopy, but provides better spatial resolution
(about 1 micrometer) and allows one to work through glass, at two or three
times the cost ($150K to $250K. For this money, one might also consider an
FTIR microscope equipped with a focal plane array detector.

Please feel free to contact me off-line if you have questions.

Jamie

James Martin
Orion Analytical, LLC
www.orionanalytical.com
martin-at-orionanalytical.com
413-458-0233
fax 413-458-5542


----- Original Message -----
} From: "Jeff Hedlesky" {jhedlesky-at-edgebb.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 29, 2001 1:12 PM


Hi, Bill-

} I'm having a problem with my MT-1B Ultra microtome. When cutting ultra thin
} sections my microtome skips cutting a section completely then cuts a section
} that's too thick. Any solutions? I can't find a company that services
} Sorvall MT-1B microtomes. Anyone have a source for service calls.

Where are you located? Nowhere near me, I'll bet, but there are still a
few people out there who know their way around an MT-1.

Sometimes skipped sections means you are actually trying to section too
thin, so you miss one and then the next one is thicker, then you miss
again. Try sectioning thicker until you get every section, and see if they
are of a reasonable thickness.

As for the microtome, open it up and take a look inside. It's relavtively
simple (as compared to current models) and you may see something
obvious. Check belts for wear and cracks, see if moving parts are
dirty or chipped, etc. They are mechanical marvels unencumbered by
electronics!

Good luck,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Aug 30 15:36:48 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 30 Aug 2001 16:24:59 -0400 (EDT)
Subject: Re: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Greg--early 1970s. I purchased a Coates and Welter FE SEM in
1973. It was not the first FE model, but was the first scope to come off
their line with the gun mounted on a cabinet separate from the controls
(at my request). They subsequently went to that style for stability.

It operated "cold," i.e., at room temperature, and the electrons were
extracted with the "field" below. If the tip became contaminated, the way
to remove crud was to warm it up some (I don't remember the temperature,
but it wasn't hot.) to burn away the contaminants.

It worked great from the perspective of length of filament life, no
vacuum problems, high resolution, and low voltage capability, but getting
service from the other coast when needed took forever. Frequently, I
would describe a problem, and the engineer would ship me a board. We
kept the SEM for about 10 years and sold it back to the company for
parts. But we got some great shots with higher resolution (around 50
Angstroms routinely) than anybody else was getting at that time, and it
was guaranteed at 100 Angstroms.

The company exchanged hands several times: first C & W: then Welter
bought it out; then Coates bought it back. Then it became Nanometrics,
and somebody else (?) bought the SEM technology. Maybe one of the present
sales reps can help with the recent history--it might be LEO???

Hope this answers your question.

(I have no commercial interest in any of this.)

Sara


On Thu, 30 Aug 2001, Greg Strout wrote:

} Date: Thu, 30 Aug 2001 09:30:40 -0500
} From: Greg Strout {gstrout-at-ou.edu}
} To: Angela Klaus {avklaus-at-amnh.org}
} Cc: Microscopy List Server {Microscopy-at-sparc5.microscopy.com}
} Subject: Re: FE-SEM: Historical Question
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking at an advertisement from Coats and Welter Instruments for "The
} world's first commercial field emission scanning electron microscope." It
} is touted as ...operating at room temperature and not being subject to
} burnout as are the conventional hot filament electron emitters...
} While the ad is not dated it does reference a paper from 1968 so it was
} probably produced sometime in the late 60's or very early 70's.
} Greg
}
} Angela Klaus wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hi Listers...
} }
} } Does anyone know when the first cold field emission microscope became
} } commercially available?
} }
} } Thanks and best,
} }
} } Angela
} }
} } Angela V. Klaus
} }
} } Director, Interdepartmental Laboratories
} } American Museum of Natural History
} } Central Park West at 79th Street
} } New York, NY 10024 USA
} }
} } Email: avklaus-at-amnh.org
} } Tel: 212-769-5977
}
} --
} ==================================================================
} Greg Strout
} Electron Microscopist, University of Oklahoma
} WWW Virtual Library for Microscopy:
} http://www.ou.edu/research/electron/www-vl/
} e-mail: gstrout-at-ou.edu
} Opinions expressed herein are mine and not necessarily those of
} the University of Oklahoma
} ==================================================================
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Aug 30 15:36:48 2001



From: Raymond Bennett :      rbennett-at-hortresearch.co.nz
Date: Fri, 31 Aug 2001 08:31:51 +1200
Subject: Re: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




{italic} Howdy;


We here at the end of the world had a Coats and Welter Field
Emission SEM which was purchased in 1972.

It produced fantastic results until 1985 when we had to turn it off
due to lack of spare parts including the tips!


In fact; when trying to phone the company in USA that we
purchased the tips from.......it just rung at a dead end!!


Cheers

Raymond Bennett


Keith Williamson EM Unit

HortResearch

Private Bag 11030

Palmerston North

NEW ZEALAND




Date sent: {/italic} {color} {param} 0000,0000,8000 {/param} Thu, 30 Aug 2001 09:30:40 -0500 {italic} {/color}

} From: {/italic} {color} {param} 0000,0000,8000 {/param} Greg Strout { {gstrout-at-ou.edu} {italic} {/color}

{bold} {/italic} Subject: {color} {param} 0000,0000,8000 {/param} Re: FE-SEM: Historical Question {italic} {/bold} {/color}

To: {/italic} {color} {param} 0000,0000,8000 {/param} Angela Klaus { {avklaus-at-amnh.org} {italic} {/color}

Copies to: {/italic} {color} {param} 0000,0000,8000 {/param} Microscopy List Server { {Microscopy-at-sparc5.microscopy.com} {italic} {/color}


------------------------------------------------------------------------

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-----------------------------------------------------------------------.



I am looking at an advertisement from Coats and Welter Instruments for "The

world's first commercial field emission scanning electron microscope." It

is touted as ...operating at room temperature and not being subject to

burnout as are the conventional hot filament electron emitters...

While the ad is not dated it does reference a paper from 1968 so it was

probably produced sometime in the late 60's or very early 70's.

Greg


Angela Klaus wrote:


{/italic} {color} {param} 7F00,0000,0000 {/param} } ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com

} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

} Hi Listers...

}

} Does anyone know when the first cold field emission microscope became

} commercially available?

}

} Thanks and best,

}

} Angela

}

} Angela V. Klaus

}

} Director, Interdepartmental Laboratories

} American Museum of Natural History

} Central Park West at 79th Street

} New York, NY 10024 USA

}

} Email: avklaus-at-amnh.org

} Tel: 212-769-5977


{italic} {/color} --

==================================================================

Greg Strout

Electron Microscopist, University of Oklahoma

WWW Virtual Library for Microscopy:

http://www.ou.edu/research/electron/www-vl/

e-mail: gstrout-at-ou.edu

Opinions expressed herein are mine and not necessarily those of

the University of Oklahoma

==================================================================




{nofill}


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Thu Aug 30 17:29:29 2001



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 30 Aug 2001 17:21:07 -0500
Subject: spectroscopy and spectrometry, language

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
I would like to add a few words on the thread about
spectroscopy vs spectrometry. Bill Tivol defined (see below)
spectroscopy as producing spectra for visual inspection and
spectrometry as producing spectra for measurment.

This is perfectly rational, nice and tidy, but like so many
other things in language, does not seem consistent with usage. The
OED defines spectroscopy as the study of spectra and does not have an
entry for spectrometry. My big Websters (II ed, yes thanks) lists
both, as does Collins, and both are defined as the science and study
of spectra. And I would argue that there are really relatively few
instances of a pure spectroscope, where all you do is look at the
spectra, and equally few instances of a pure spectrometer (where you
never see the spectra--think of all those peaks on the "EDS" computer
screen). Basically, as spectroscopists (are there any
spectrometrists??) we have the job of producing, interpreting, and
measuring spectra, and spectroscopy has been used for years as a word
to define that act. The word spectrometry has likewise been used, but
I doubt whether the measuring-observing distinction can be reliably
applied. If given a choice, I would use spectroscopy for the lot
because it is the older word. But I suspect that various sub species
have their own convention (thus it is always as far as I know FTIR
microspectroscopy; and perhaps always energy dispersive spectrometry)
and these fields will keep their conventions.

As ever,
Tobias Baskin

}
} When I was shopping for one, I was corrected by someone along the way that
} the "S" in EDS stands for spectrometry, not spectroscopy. Several books
} here in the lab library also use that term.
}
} Before I confuse part of the next generation of microscopists this
} upcoming semester, is one term better than the other - or is it a potato,
} potatoh, tomato, tomahtah (let's call the whole thing off) kind of thing?
}
} Dear Heather,
} The "scopy" part has to do with observation, whereas the "metry" part
} has to do with measurement (i.e., quantitation), so energy-dispersive
} spectroscopy is separating the photons by energy in order to look at the
} spectrum, and E-D spectrometry is separating the photons in order to
} perform measurements. I can understand a vendor insisting that the
} instrument is capable of quantitation and is, thus, a spectrometer.
} Yours,
}
} Bill Tivol
} Wadsworth Center
} Albany NY
} (518) 473-7399 WFT02-at-health.state.ny.us

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123


From daemon Thu Aug 30 19:00:31 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 31 Aug 2001 09:55:09 +1000
Subject: RE: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In 1976 both, Siemens and Vacuum Generator marketed FESEMs. The Vacuum
Generator unit was considerably cheaper and possibly better too.
Incidentally, the Siemens unit became a fiasco and likely was a major reason
for the company's board to abandon the building of electron microscopes a year
or two later. Siemens had been the first commercial manufacturer of electron
microscopes and built the best instruments in the 50th and early 60th. The
Philips EM300 was the first serious challenge to Siemens.
Michael Beer of Chicago published a great deal of FESEM developments in the
early 70th.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, August 30, 2001 9:43 PM, Angela Klaus [SMTP:avklaus-at-amnh.org]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi Listers...
}
} Does anyone know when the first cold field emission microscope became
} commercially available?
}
} Thanks and best,
}
} Angela
}
} Angela V. Klaus
}
} Director, Interdepartmental Laboratories
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977
}



From daemon Fri Aug 31 07:02:29 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 31 Aug 2001 07:52:18 -0400
Subject: Kudo's to Nestor

Contents Retrieved from Microscopy Listserver Archives
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Listers,

Nestor's little slip and his apology should make us realize how valuable Nestor and his service of the listserver is to us. I just want to say Thank You Nestor for all the hard work you do to keep us on-line and spam free. You help all of us to stay connected in a nice environment where we can ask our questions and communicate our ideas.

Thank You Nestor!


Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Aug 31 07:27:23 2001



From: Troutt, David :      dwtroutt-at-eastman.com
Date: Fri, 31 Aug 2001 08:21:35 -0400
Subject: Kudo's to Nestor

Contents Retrieved from Microscopy Listserver Archives
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We are beginning preparations for the moving of our old Cambridge S200 SEM.
Even though the poor gal is only moving about 500 yards, the transfer is to
another building requiring removal of walls and the ever so delicate
transfer by fork lift into the back of a truck. We need information on what
is required and/or helpful in making sure the SEM successfully completes the
trip. Thanks in advance.

David W. Troutt
Corporate Analytical Services
Voridian Company
P.O. Box 1972
Kingsport, TN 37662-5150
Phone: 423-229-1993
Fax: 423-229-4558


From daemon Fri Aug 31 08:02:22 2001



From: jshields-at-cb.uga.edu
Date: Fri, 31 Aug 2001 08:56:23 -0400
Subject: Re: MT 1B Maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Contact Microtome Service Company. Although they appear to
only service MT2 &2B, they might have info or long lost spare parts
for your beast.
Good luck!
john

MTS
7568 Florian Way
Liverpool, New York 13088
315-451-1404

On 30 Aug 2001, at 13:40, Hanika, William wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} I'm having a problem with my MT-1B Ultra microtome. When cutting
} ultra thin sections my microtome skips cutting a section completely
} then cuts a section that's too thick. Any solutions? I can't find a
} company that services Sorvall MT-1B microtomes. Anyone have a source
} for service calls. Bill




From daemon Fri Aug 31 08:20:30 2001



From: Troutt, David :      dwtroutt-at-eastman.com
Date: Fri, 31 Aug 2001 09:14:39 -0400
Subject: Moving Old Cambridge SEM 200

Contents Retrieved from Microscopy Listserver Archives
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Sorry. The I left the subject line blank on the first letter blank. I
thought most folks would delete it thinking it was an advertisement for a
lifetime supply of Viagra.

We are beginning preparations for the moving of our old Cambridge S200 SEM.
Even though the poor gal is only moving about 500 yards, the transfer is to
another building requiring removal of walls and the ever so delicate
transfer by fork lift into the back of a truck. We need information on what
is required and/or helpful in making sure the SEM successfully completes the
trip. Thanks in advance.


David W. Troutt
Corporate Analytical Services
Voridian Company
P.O. Box 1972
Kingsport, TN 37662-5150
Phone: 423-229-1993
Fax: 423-229-4558


From daemon Fri Aug 31 08:54:03 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 31 Aug 2001 09:48:21 -0400
Subject: Re: spectroscopy and spectrometry, language

Contents Retrieved from Microscopy Listserver Archives
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I would argue that there are really relatively few
instances of a pure spectroscope, where all you do is look at the
spectra, and equally few instances of a pure spectrometer (where you
never see the spectra--think of all those peaks on the "EDS" computer
screen).
Dear Tobias,
Perhaps WDS comes closer to a "pure spectrometer" than EDS, since one
measures the counts for a given wavelength, then another, etc., but it is
not necessary that an actual instrument be purely one or the other for the
vendor or user to distinguish the major function by different words. In
the much-much-lower energy region, the instrument mostly used today is a
(wavelength-dispersive) spectrophotometer. Newton's original prism
instrument was a spectroscope. (I suppose that avalanche photodiodes could
be used to make an energy-dispersive spectrophotometer, but I have not
heard of such a thing.)

This is perfectly rational, nice and tidy, but like so many
other things in language, does not seem consistent with usage.

Of course, practical usage is always messier than language describing
an ideal.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Fri Aug 31 09:07:55 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 31 Aug 2001 10:00:51 -0400
Subject: RE: MT 1B Maintenance

Contents Retrieved from Microscopy Listserver Archives
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Hi Bill,
What routine service have you administered, and how often and when
last?

Fred Monson'


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Hanika, William
} Sent: Thursday, August 30, 2001 2:40 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: MT 1B Maintenance
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm having a problem with my MT-1B Ultra microtome. When cutting ultra
} thin
} sections my microtome skips cutting a section completely then cuts a
} section
} that's too thick. Any solutions? I can't find a company that services
} Sorvall MT-1B microtomes. Anyone have a source for service calls.
} Bill
}
}


From daemon Fri Aug 31 09:21:25 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 31 Aug 2001 10:15:11 -0400
Subject: MT1 Survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK, Time for some fun!

Bill (AND Tina!) just made me look inside MT-1, and I had a thought
(what pain!).
Who has one and still uses it?

If you do, just reply to this with the following information tacked
on - if you want to.
Let me know how you came to possess IT, and what you use it for that
it does better than those that are newer (and BETTER?).
Mine came from a cart on its way to the dumpster! (Imagine!), and I
use it for really difficult stuff, though not that often. Usually it
reclines in a display case so that no one else will mess with it.

After a while I'll return with a summary.

By the way, on the same cart was an MT2 with a Christensen Cryo System with
2 controllers. All still work!!! Thank you Kent! Also, they came with the
books!


Fred


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Fri Aug 31 10:03:37 2001



From: Pippa Hawes, School Chemistry :      Pippa.Hawes-at-bristol.ac.uk
Date: Fri, 31 Aug 2001 16:05:15 +0100
Subject: TEM - embedding silica

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Dear all

I have a sample of dried porous silica that I would like to embed and
thin section. Could anyone give me any advice on the best resin and
protocol to use? The idea of using a methacrylate resin has been thrown
about (the idea, not the resin - I can assure you), however I would be
very grateful for any advice regarding any of the resins available. If
this is a bit specific then reply off-line to the address below.

Thanks
Pippa
----------------------
Pippa Hawes, EMU
School of Chemistry
University of Bristol
Cantocks Close
Bristol UK
BS8 1TS
Pippa.Hawes-at-bristol.ac.uk



From daemon Fri Aug 31 10:34:44 2001



From: halcrow-at-unbsj.ca :      halcrow-at-unbsj.ca
Date: Fri, 31 Aug 2001 12:27:34 -0300
Subject: epoxy solvent

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,

I have an old bottle of Epoxy Solvent, bought from Polysciences under
their catalogue number 0638. Does anyone know what this solvent
contains? The company tells me that they cannot find this item in their
records and probably have deleted any technical or MSDS information
pertaining to it. I'd like to get rid of the approx. 300 ml of solvent left in
the bottle but don't what, if any, hazards should be considered.

Thanks for any help you could offer.

Kevin Halcrow



Dr Kevin Halcrow, Telephone (506)-648-5567
Honorary Research Professor, Fax (506)-648-5811
Department of Biology, EMail Halcrow-at-unbsj.ca
University of New Brunswick,
P.O. Box 5050,
Saint John, NB
Canada, E2L 4L5



From daemon Fri Aug 31 10:41:44 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Fri, 31 Aug 2001 12:04:59 -0400
Subject: RE: Ammonium acetate & virus prep.

Contents Retrieved from Microscopy Listserver Archives
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$0.000001 worth! My experience is that once a faculty member ascends to an
administrative post his first instruction concerning his previous colleagues
is that each is a cost center to be discounted as rapidly as possible to the
lowest possible value. There is nothing that remains in the administrative
jargon (Bill of Administrative Rights) that is not focused on gibberish and
avoidance of responsibility. Since that philosophy reposes at the highest
academic levels, it is no wonder what is being found somewhat lower down.

At academic institutions libraries are called resources, but they are
treated as cost centers! If core labs have positive bottom lines, they are
only ephemeral (with support from self-accounting measures). Such a
situation can only occur when there is an influx of direct financial
support to individuals linked (100%) to those facilities. If the grant
winners are affiliated with departments, the departments split the gravy,
and the core remains a cost center. Whatever support the core gets in large
institutions is NOT direct. For the most part such support will ultimately
derive from indirect monies. In this way the administrator can claim that
the institution provides "direct" support ("Most of the institutional
support derives from our commitment to excellence for all of our academic
and research commitments," she said at the convocation.) leaving the core
as a cost center - gaining no "credit" for its part in raising the money on
which it lives. At how many places does the investigator pay and then repay
for the use of research animal facilities. When indirect monies can be in
the 70%'s of direct costs (usually salaries + benefits) on the grant budget,
there is BIG money involved. For example, for $50,000 in salary and
benefits to the researcher, 70% indirect costs would give $35,000 more to
the institution to use almost as it pleases. This matter is purposefully
complicated by the fact that each institution must negotiate with the
government for its percentage and coverage. No matter what one says,
however, personal experience will almost always not be universally
applicable.

Indeed, that admission would suggest that there may still exist Deans who
believe that their sole purpose is to serve the faculty at the pleasure of
the President (and the President is second)! For the most part today, such
individuals are not permitted to survive in administrative positions. For
example, it was not the student who changed the University/College in the
'60's, it was the administrator who saw a dwindling student population as a
threat to the institution and translated the threat downward to the faculty
member who was causing students to flunk out or keeping them from getting
into medical school. Children immediately sensed this trend and happily
blamed professors for their academic problems. Administrators began to
depend on the course evaluations for evaluating faculty performance.
Faculty members adapted, because they had no other recourse. Students
sensed the trend and became even more militant. Faculty dropped standards,
because they had no other choice. Students became unaware of the diminution
of standards, having arrived with expectations and demands. Faculty were
already bottomed out, and no one could do anything when teaching ended up
"in the basement". If a student couldn't understand a math instructor from
Eastern Europe, he/she knew that any complaint would be treated as
discriminatory and thus, patiently ignored. If the instructor couldn't
teach, students always had the option to take the same course with one of
the other faculty in the department. Having two or more classes with 24
students each (the administrative holly grail) taking the same course with
the same book never caused competition between instructors for the better
course evaluation. (I've seen professors who cry about their love for the
student and preen about their great evaluations promoted while their much
more erudite colleagues are left behind. Then they become administrators!)
Academic administrators. A very interesting group of folks.

The best example of adminstrator malfunction in our business is the "new"
building mentality that came out of the new construction boom from the 60's
to the '80's. For every new building there is an administrator who will
turn off the cooling system in the Fall and the heating system in the
Spring, especially when informed that such "air" conditioning systems are
designed to have both functioning at once all of the time ("That's why we
have sealed windows!"). There are even a few who have turned off the heat
during Christmas (does anybody remember that mid-year break?) and let the
plumbing freeze.

I once worked in a brand new building whose internal fire doors were tested
a few weeks after occupancy. On attempting to re-open them, the new owners
found that some of the ADMINISTRATIVE changes to the building design had
removed the access doors. About half of the fire doors remain closed for
the life of the building.

I worked at a large Eastern Academy for many years. During that time, the
greatest pleasure was to be so distanced from academic administrators that I
never knew any by face. Of course, one always managed to receive
individualized or generic administrative threats but NEVER the
administrative accolades (even for BIG grant awards!). Even with a large
government grant, one is considered a cost center!

Big or small, the academic administrator will remain one thing for certain.
He or she was one of us!

} ----------
} From: Matthew Lynn
} Reply To:
} Sent: Thursday, August 30, 2001 11:25 AM
} To: 'Microscopy-at-MSA.Microscopy.com'
} Subject: RE: OEM service vs. Insurance Companies---Long message
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Ken,
}
} I think we would ALL love to see tuition supporting
} inter-departmental facilities. Departments are not profit
} centers per se, but may be expected to be self-sufficient.
} And from what (little) I'm learning about administrative
} thought processes...your childrens' tuition is essentially
} "credited" to the departments (or school) in which they
} major. Maybe not directly as hard dollars, but in terms of
} "weight" to be thrown around :) Inter-departmental,
} multi-user facility, interdisciplinary....great buzzwords
} but no real money.
}
} My $0.02...based on broad impressions of OTHERS'
} experiences. (I don't yet know whether I am describing the
} views of my employer....)
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} mlynn-at-miami.edu
}
}
} On Wednesday, August 29, 2001 9:09 PM, Ken Converse
} [SMTP:qualityimages-at-netrax.net] wrote:
} } Matt,
} } I'll repeat what I said to Phil Oshel:
} } If I found a school turning any profit on a service lab,
} } I'd scream
} } bloody murder as I've got 2 in college and 2 on the way.
} } I would expect
} } my tuition bills to go a long way towards general
} support.
} }
} }
} } My take as a parent.
} }
} } Ken Converse
} } owner (and parent of 4)
} } Quality Images
} } third party SEM service
} } Delta, PA
} }
} }
} } Matthew Lynn wrote:
} }
} } }
} } }
} ---------------------------------------------------------
} } } ---------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy
} } } Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } }
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} } } ml
} } }
} } }
} ---------------------------------------------------------
} } } --------------.
} } }
} } }
} } } Ken,
} } }
} } } I'll add my thanks for your viewpoint. The problem we
} } } in
} } } Universities are having is this: you wrote
} } }
} } } } "University service
} } } } labs are not supposed to be profit centers. They are
} } } } SERVICE labs and
} } } } are heavily subsidized...."
} } }
} } }
} } } Sadly, survey says that very few of us have
} } } administrators
} } } who share your viewpoint. You make excellent points
} } } about
} } } all of the options, and we can only hope this entire
} } } discussion helps when renewal time comes around.
} } } Perhaps
} } } the OEM Service departments need to "suit up", bring a
} } } fancy presentation, and state their case against the
} } } ICs.
} } }
} } } Matt
} } }
} } } Matthew J. Lynn
} } } Center for Advanced Microscopy
} } } University of Miami
} } } (305)284-4736
} } } mlynn-at-miami.edu
} } }
} } }
}
}
}


From root Fri Aug 31 11:12:21 2001
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Dear Karen:
After touching the grid to the drop containing viral particles, I draw off
most of the fluid then add a drop of the 1 mM sol of ammonium acetate. If
you find that you still end up with remains of dried buffer solution, try
an additional change of ammonium acetate rinse. Never let the drop of any
solution you add to the grid to dry until you reach the final drop of stain
application.
If you don't already use self-closing, anticapillary tweezers, I would
recommend them for minimizing carry over between solution changes.
The advantage of using PTA and ammonium molybdate negative stains is that
their pH can be adjusted.
PTA is known for giving good contrast. I use a 1 N KOH solution to adjust
the pH. I use a final stain concentration of 1% PTA. Ammonium molybdate
although it has less contrast, gives a very fine grain appearance which is
good for small detail at high magnifications.
I use a final concentration of 1% aqueous uranyl acetate negative stain
which yields more contrast than ammonium molybdate. It also has the
advantage of acting as a positive stain for chromatin material.
Be aware that phosphate and cacodylate are precipitated by uranyl salts. So
phage containing these buffers should undergo a number of droplet rinse
changes to remove any traces of the buffer before staining.
Good luck.
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
State University of New York-Binghamton
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu



From daemon Fri Aug 31 11:28:49 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 31 Aug 2001 09:23:50 -0700 (PDT)
Subject: Electroscan E3 SEM question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I've been going through setting up an Electroscan ESEM E3 in our
laboratory and vacuum problems aside, we now have a stage that doesn't
want to move. Anyone ever come across stage problems and have some
suggestions that I may try for this instrument?

The stage will actually move if you give it a gentle push along a gear
arm, but it often locks up in the Z direction, and sometimes X/Y. Auto
calibrate allows the stage to move in X/Y, but it never calibrates the Z
direction.
Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Aug 31 11:32:00 2001



From: K.N. Bozhilov :      bozhilov-at-citrus.ucr.edu
Date: Fri, 31 Aug 2001 09:27:29 -0700
Subject: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I would greatly appreciate if you could share your opinion about performance
and reliability of the Bench Top Turbo III vacuum evaporator from Denton
Vacuum, also alternative suggestions are more than welcome. We are looking
currently to purchase a vacuum evaporator for general EM preparation capable
of carbon and metal evaporation, glow discharge and thickness control.
Cleanliness of the vacuum is our main concern and especially the fact that
the Bench Top model does not have LN trap.

Thank you,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324



From daemon Fri Aug 31 11:50:01 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Aug 2001 09:47:06 -0700
Subject: Re: LM - Something I just don't get

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



To all who asked about this article, I have scanned it
and saved it as an Adobe PDF file. It can be downloaded
from http://gaugler.com/lmarticle_1.pdf using your
browser.

If you have any problems, please let me know. In PDF,
the whole article is about 1.33MB file size.

gary g.




At 02:21 AM 8/28/2001, you wrote:


} Dear Gary,
} I'd be interested in this article, but do not have
} access to the Biological Bulletin.
} Do you have the articlein electronic form, or is ther
} a URL that you can point me to?
}
} Regards, Jeremy Sanderson
} jb_sanderson-at-yahoo.com
}
}
} } Regarding this subject, the local Zeiss rep gave me
} } a
} } copy of an interesting and valuable article that
} } hits
} } right on the topic. The article is "Choosing
} } Objective
} } Lenses: The importance of numerical aperture
} } and magnification in digital optical microscopy."
} } Piston, D. (August, 1998). The Biological Bulletin,
} } 195/1.
} }
} } Anyone interested should look this up. It also
} } talks
} } about digital capture and the relationship to laser
} } scanning confocal microscopes.
}
} } gary g.
} }
} }
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Fri Aug 31 12:27:43 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 31 Aug 2001 10:19:47 -0700
Subject: SEM: YAP scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
considering using a YAP scintillator instead of the typical phosphorous
scintillator. Does anyone have any experience or suggestions on this? YAP is
supposed to have a better S/N ratio, which is appealing to me. Most of my work
is at 10kv on non-conducting oxides and at moderate magnification, however I'd
really like improved performance at lower voltages, smaller spot sizes, and
higher magnification, where the S/N ratio becomes a problem with my current
system. Thank you for any input.


Brad Johnson
Pacific Northwest National Lab
P.O. Box 99, K6-24
Richland, WA 99352
voice: 509-372-1635
fax: 509-376-3108

Bradley.Johnson-at-pnl.gov


From daemon Fri Aug 31 15:30:06 2001



From: Weiliang Gong :      gongw-at-vsl.cua.edu
Date: Fri, 31 Aug 2001 15:06:53 -0500 (EST)
Subject: A Suggestion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, List members,

Thanks are given to Nestor and many people whom I do know but help me a
lot. I have been in this list for several years. There have been many
great articles on electron microscoy, sample preparations and
knowledges of instruments. I collected some of them, really valuable. I
saw there were some summarizing articles on specific questions in the
past. Do we have soemone who are classify technical articles and store
somewere in an internet server? I saw questions raised on repeated
subjects. If we have a server stored old, valuble articles for list
members to access, that will be great. Recently, we decided to buy an
electropolisher for our lab. We obtained several responses offline and
they were really useful and presented clearly the advantages and
disadvantages of each brand machine. I will write a summary. We
believed that we made the right decision.

Thank you for your attention.

Good weekend,

Weiliang Gong
Vitreous State Laboratory
Catholic University of America
Washington, D.C. 20064


From daemon Fri Aug 31 16:38:58 2001



From: Mark Germani :      mgermani-at-micromaterialsresearch.com
Date: Fri, 31 Aug 2001 16:36:19 -0500
Subject: Transferring files from Tracor Northern systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be interested in hearing from anyone with experience transferring
files from Tracor Northern (Noran) 5400 or 5500 systems. Many years ago I
had written a FORTRAN program running on a DEC computer to accept Data (type
4) files sent using the TN XI module. I would like to be able to transfer
TN spectral and image files to a Mac or PC but I am not sure of the file
formats nor of a good communications program to use. If you have written
software that you would be willing to share or purchased software that you
no longer use and would like to sell, then please contact me.

Mark Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive, #200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 fax

mgermani-at-micromaterialsresearch.com





From daemon Fri Aug 31 18:02:17 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Aug 2001 18:57:25 -0500
Subject: TEM on porous silicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Pippa Hawes wrote:
================================================================
I have a sample of dried porous silica that I would like to embed and thin
section. Could anyone give me any advice on the best resin and protocol to
use? The idea of using a methacrylate resin has been thrown about (the idea,
not the resin - I can assure you), however I would be very grateful for any
advice regarding any of the resins available. If this is a bit specific
then reply off-line to the address below.
================================================================
We have always found that samples of this type (being run in our laboratory
analytical services division), section quite nicely in most of the so-called
"Epon® substitute" resins such as our own SPI-Pon™ Embedding Kit. We would
expect that the "substitutes" offered by our main competitors would probably
work just as well.

However, porous silica must be vacuum embedded, otherwise it can't be
sectioned very well, if at all. We have never been able to get the section
quality and stability with methacrylates or any system other than the "Epon"
type kits. If one is working with "wet" silicas, and drying is not an
option (as it sometimes is), then we have used our SPI-Chem™ Low Acid GMA as
an embedding resin (which requires no dehydration).

The "Epon substitute" kits all come with recommended protocols, and while
there is some variation between them, my guess is that any one protocol will
work just as well with any of the other "substitutes". One advantage of
the "Epon" systems is that the hardness can be varied quite easily, and
often times the section quality can be improved by varying the hardness of
the cured block.

Another protocol comment is that one can not use glass knives very easily
(if at all) on this kind of sample, we use only (materials science) diamond
knives.

Disclaimer: SPI Supplies, through our Structure Probe® laboratory services,
perform these kinds of sample preps and TEM analysis for clients. We also
are major suppliers of epoxy embedding resins and the SPI brand of materials
science diamond knives.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Fri Aug 31 18:12:16 2001



From: Dmrelion-at-aol.com
Date: Sat, 1 Sep 2001 06:51:29 EDT
Subject: spectroscopy and spectrometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hello,
} I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
} considering using a YAP scintillator instead of the typical phosphorous
} scintillator. Does anyone have any experience or suggestions on this?

About ten years ago I ran some tests for signal on several scintillator
types: YAP, YAG, standard aluminum coated scintillator & "uncoated"
scintillator.
The result at that time were surprising.

Using a JEOL 35C SEM & running the same specimen current & PMT voltage with
each scintillator the best signal was given by the original aluminum coated
scintillator.

The second best was the "uncoated" scintillator & it was only 80% of the
signal the standard aluminum coated scintillator.
The YAP & YAG gave only 60% of the standard aluminum scintillator.

I was very disappointed as the YAP & YAG were significantly more than the
standard scintillator.
The conclusion that I came to was that the YAP & YAG would probably give
more longevity & could justify their extra cost but they were not fit for
high resolution. The YAP & YAG would probably be suitable for probe work.

Keep in mind this experiment was done about ten years ago. Perhaps the
crystals have improved in recent years & the data may need a re-evaluation.
I am sure that there is someone who would be willing to criticize my results
(maybe in Northern Calif.?) but this the data that I received at the time.

Regards,


Earl Weltmer


----- Original Message -----
} From: "Johnson, Bradley R" {Bradley.Johnson-at-pnl.gov}
To: "'Microscopy-at-MSA.Microscopy.com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 31, 2001 10:19 AM


I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sat Sep 1 07:17:06 2001



From: Dmrelion-at-aol.com
Date: Sat, 1 Sep 2001 08:10:07 EDT
Subject: spectrometers and spectroscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sat Sep 1 10:29:40 2001



From: Beauregard :      beaurega-at-westol.com
Date: Sat, 01 Sep 2001 11:18:30 -0400
Subject: Re: TEM - embedding silica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Pippa,

You do not state the exact type of silica you are embedding. SO...

In general methacrylates, whether LR White or such, will shrink much more
than an epoxy like one of the EPON CLONE formulations. If I recall 20-25%
shrinkage is what you get with LR White and a lot of polymers. Epoxy
formulas are more like 5%. This lower shrinkage is preferred by me so that
I can argue to customers that I have not changed the microstructrure
through severe shrinkage during the polymerization. LR White is faster
(with an accelerator) curing, if you are in a hurry. The EPON clone stuff
takes overnight curing in an oven at 60 to 70 C. It's no big deal because
you just prepare the samples at the end of the day and start sectioning in
the morning.

I have personnaly done precipitated silicas, arc silicas, and fumed silicas
using Epon. Silica fume should be no problem. I do not vacuum embbed
these materials. Ppt'd silicas have a surface area of about 154-250 meters
squared per gram and the epon will wet almost every pore between the
primary or ultimate particles without vacuum. Capillary forces are strong
enough on our products and others to wet almost 100% of what is there. One
of the components in my EPON kit foams under partial vacuum and that is
another reason I don't use vacuum.
You might have to use vacuum in your case. However, test each EPON kit
component separately to see how much vacuum it can tolerate before foaming.

We have made various sizes of agglomerated spheres of these particles since
the mid 70s as I recall. Anyway, they can be fragile but epon does not
break up the microstructure. One can section throgh the 'micro-dust' on
the surface of these spheres without loss of material.

As you can see, EPON works on a wide range of 'porous silica' samples.
There must be a reason why EM people like it? It works on a wide range of
materials, wets well, sticks fairly well to them, and sections well.

Eponate 12 releases from polyethelene molds, bottle caps, etc. quite well
for 1-4 cycles of mold use with EPON, FYI.

Hope this helps.

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15146

Opinions given are my own and not those of PPG Industries.


} Dear all
}
} I have a sample of dried porous silica that I would like to embed and
} thin section. Could anyone give me any advice on the best resin and
} protocol to use? The idea of using a methacrylate resin has been thrown
} about (the idea, not the resin - I can assure you), however I would be
} very grateful for any advice regarding any of the resins available. If
} this is a bit specific then reply off-line to the address below.
}
} Thanks
} Pippa
} ----------------------
} Pippa Hawes, EMU
} School of Chemistry
} University of Bristol
} Cantocks Close
} Bristol UK
} BS8 1TS
} Pippa.Hawes-at-bristol.ac.uk
}
}
}
}



From daemon Sat Sep 1 10:57:01 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Sep 2001 11:53:04 -0500
Subject: YAG and YAP scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Earl Weltmer and Brad Johnson wrote:
============================================================
} Hello,
} I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
} considering using a YAP scintillator instead of the typical phosphorous
} scintillator. Does anyone have any experience or suggestions on this?
++++Brad Johnson

About ten years ago I ran some tests for signal on several scintillator
types: YAP, YAG, standard aluminum coated scintillator & "uncoated"
scintillator.
The result at that time were surprising.

Using a JEOL 35C SEM & running the same specimen current & PMT voltage with
each scintillator the best signal was given by the original aluminum coated
scintillator.

The second best was the "uncoated" scintillator & it was only 80% of the
signal the standard aluminum coated scintillator.
The YAP & YAG gave only 60% of the standard aluminum scintillator.

I was very disappointed as the YAP & YAG were significantly more than the
standard scintillator.
The conclusion that I came to was that the YAP & YAG would probably give
more longevity & could justify their extra cost but they were not fit for
high resolution. The YAP & YAG would probably be suitable for probe work.

Keep in mind this experiment was done about ten years ago. Perhaps the
crystals have improved in recent years & the data may need a re-evaluation.
I am sure that there is someone who would be willing to criticize my results
(maybe in Northern Calif.?) but this the data that I received at the time.
+++++ Earl Weltmer
============================================================
We have offered P-47, YAG and YAP scintillators for SEM applications for
more then twenty years. Earl is correct that the P-47 performance, **but
when first installed**, does probably give a superior performance. However
, not all YAG and YAP crystals come out of the "same cookie cutter." From
what we have been able to deduce, a high quality YAG or YAP single crystal
scintillator will perform so close to that of the P-47 that one usually has
difficulty detecting the difference. However, as everyone knows, the P-47
once installed, in terms of performance, goes only down. The only question
is how fast it will go down. And that rate of deterioration of performance
depends among other things on the type of work being done. For example, the
higher x-ray fluxes associated with EDS (and especially WDS) tend to cause a
more rapid deterioration.

Also, if Earl's experiment was done as described, and if a YAG was tested
against P-47 but using the same PMT, then one would expect to find an
inferior performance, because the PMT that is optimum for P47 is not the
same one that would have been optimum for YAG. Now this might be less of a
problem today than it would have been ten years ago, but if the PMT was not
changed, then the right test (IMHO) was not the one being performed. To
make the comparison, one would have had to have taken out the S11 style and
replaced it with an S20 PMT. This point is further explained on our website
URL
http://www.2spi.com/catalog/scintill/spi-yag.html

When a YAG or YAP scintillator is installed, its performance level is
constant and does not deteriorate. So while the comparison might suggest
some superiority at the very beginning, in most instances that we have seen,
or have been led to believe, it is not long that the SEM running on YAG or
YAP in general, is operating at a higher level of performance. And the SEM
is not subjected to the downtime associated with the need to change a P-47
powder scintillator from time to time.

For those operating at low KV in the BSE mode (and using YAG or YAP), and
high magnifications, the image is clearly less noisy. A collection of ten
different references from different laboratories around the world, which
have been collected on URL
http://www.2spi.com/catalog/scintill/sem-tem.html
at least to some, pretty much document the superiority of the single crystal
scintillators over the powder scintillators. I guess one could always try
to do more studies, but anyone that is familiar with these publications
finds their conclusions pretty persuasive.

We have generally recommended that the SPI single crystal scintillators
offered many advantages over the more traditional P-47 scintillator. We
have been under the impression that those who have made the switch have been
pretty happy with their results, though we do hear from time to time of
someone who would not agree with that statement. Of course, the performance
is related to the nature of the doping of the crystal, and as I said, not
all these crystals come out of the same cookie cutter. Not all YAG's and
YAP's are created equal. So when making comparisons, and statements about
their scintillators, it would be helpful for one to state the brand of their
scintillator crystal. These crystals are certainly not a generic entity
where all are the same irrespective of the source.

Disclaimer: SPI Supplies is a major supplier of scintillators for SEM
applications including P-47's, YAGs and YAPs.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Sep 1 10:58:33 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 1 Sep 2001 08:53:38 -0700
Subject: Re: TEM - embedding silica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} I have a sample of dried porous silica that I would like to embed and
} thin section. Could anyone give me any advice on the best resin and
} protocol to use? The idea of using a methacrylate resin has been thrown
} about (the idea, not the resin - I can assure you), however I would be
} very grateful for any advice regarding any of the resins available. If
} this is a bit specific then reply off-line to the address below.
}
Pippa -

You'll probably do fine with LR White, Hard grade. Its low viscosity is a
big help, but if the sample floats anyway you may need vacuum. If you've
never used that resin, be cautious when selecting the embedding mold; it
will dissolve some plastics & it won't harden in flat molds unless air is
excluded. Gelatin capsules work.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Sep 1 21:00:34 2001



From: Dmrelion-at-aol.com
Date: 09/01/2001
Subject: spectoscopy and spectrometry

Contents Retrieved from Microscopy Listserver Archives
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Subj: spectrometers and spectroscopes

I have followed this discussion with some interest. A similar situation
exists in the field of mass spectroscopy.

At one time there were many mass spectrographs and mass spectrometers being
used. Mass spectrographs were those instruments in which the detected ions
were spread out along a focal plane and a significant portion of the mass
range was collected simultaneously on a photographic plate. Mass
spectrometers were those instruments in which the detected ions were moved
across a detector slit (by varying magnetic field or ion accelerating
potential) and collected/detected, one m/q at a time, by either a Faraday
cup or electron multiplier after the slit, providing a readout on an
electronic device (electrometer usually). Mass spectroscope or mass
spectroscopy was used as the general term to include either type of
instrument.

Mass spectrographs are rarely seen any more and even if they are used, the
photographic plate would probably be replaced by some type of CCD detector
providing electronic readout so the original clear distinction becomes
blurred.

I'm sure that an analogous situation exists in optical spectroscopy.

Don Marshall



Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Sun Sep 2 00:09:15 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 2 Sep 2001 15:02:52 +1000
Subject: RE: FE-SEM: Historical Question

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Message-ID: {01C133C0.5859C4A0.jim-at-proscitech.com}
"jim-at-proscitech.com"
{jim-at-proscitech.com} ,
"'Angela Klaus'" {avklaus-at-amnh.org} ,
"microscopy-at-sparc5.microscopy.com" {microscopy-at-sparc5.microscopy.com}


Reinhard Rachel also wrote on this back-channel and so I expect that you are
right: I confused the early FE-SEM and FE-TEM.
I saw the Siemens instrument in the Karlsruhe application lab in October 76. I
was there for two other instruments but the large FE instrument was of course
rather eye-catching. 25 years later I recall that it had a short, but large
diameter column and so thought of it in the long-term as a FE-SEM.
Hope that this was not a complete waste of time as these contributions have
placed other FE instruments in a historical context.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, September 01, 2001 6:18 AM, Alan Nicholls [SMTP:nicholls-at-uic.edu]
wrote:
} Jim
}
} I think you mean FE-STEMs not FE-SEMs. The first VG HB5 STEM was delivered
} in 1973 to University of London.
}
} Regards
}
} Alan
}
} At 09:55 AM 8/31/2001 +1000, Jim at Proscitech wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } In 1976 both, Siemens and Vacuum Generator marketed FESEMs. The Vacuum
} } Generator unit was considerably cheaper and possibly better too.
} } Incidentally, the Siemens unit became a fiasco and likely was a major reason
} } for the company's board to abandon the building of electron microscopes a
} } year
} } or two later. Siemens had been the first commercial manufacturer of electron
} } microscopes and built the best instruments in the 50th and early 60th. The
} } Philips EM300 was the first serious challenge to Siemens.
} } Michael Beer of Chicago published a great deal of FESEM developments in the
} } early 70th.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Thursday, August 30, 2001 9:43 PM, Angela Klaus [SMTP:avklaus-at-amnh.org]
} } wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi Listers...
} } }
} } } Does anyone know when the first cold field emission microscope became
} } } commercially available?
} } }
} } } Thanks and best,
} } }
} } } Angela
} } }
} } } Angela V. Klaus
} } }
} } } Director, Interdepartmental Laboratories
} } } American Museum of Natural History
} } } Central Park West at 79th Street
} } } New York, NY 10024 USA
} } }
} } } Email: avklaus-at-amnh.org
} } } Tel: 212-769-5977
} } }
}
} Alan W Nicholls, PhD
} Director of Research Service Facility (Electron Microscopy)
} Research Resources Center - East (M/C 337)
} Room 100 Science and Engineering South Building
} The University of Illinois at Chicago
} 845 West Taylor St
} Chicago, IL 60607-7058
}
} Tel: 312 996 1227
} Fax: 312 996 8091
} Office: Room 110
}
} Web site www.rrc.uic.edu


From daemon Sun Sep 2 02:00:47 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sun, 02 Sep 2001 10:53:41 -0400
Subject: Re: Kudo's to Nestor

Contents Retrieved from Microscopy Listserver Archives
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Dear Gary,
I will receive my Axiopklan only on next week - so I have still no
experience and cannot help.
With best wishes
Jiri Kalvoda
----- Original Message -----
} From: Gary Gaugler {gary-at-gaugler.com}
To: {jsharp-at-zeiss.com}
Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, August 22, 2001 5:09 AM


Here, here! (or is it hear , hear?)
Ken Converse
Quality Images

Paula Sicurello wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers,
}
} Nestor's little slip and his apology should make us realize how valuable Nestor and his service of the listserver is to us. I just want to say Thank You Nestor for all the hard work you do to keep us on-line and spam free. You help all of us to stay connected in a nice environment where we can ask our questions and communicate our ideas.
}
} Thank You Nestor!
}
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax
}
}
}
}



From daemon Sun Sep 2 15:28:19 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Sun, 2 Sep 2001 16:19:46 -0400
Subject: NYMS Abbe award to Joseph I. Goldstein

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-----Original Message-----
} From: John A. Reffner [mailto:JAReffner-at-compuserve.com]


On Tuesday, October 2, 2001, Dr. Joseph I. Goldstein will receive the New
York Microscopical Society's Ernst Abbe Memorial Award at a special
symposium in Atlantic City, New Jersey. The Symposium is part of the
Eastern Analytical Symposium & Exposition. The session starts at 9:00-am
with the presentation of the Abbe Award. The program includes:

Joseph I. Goldstein Advances in Scanning Electron Microscopy and
X-Ray
Microanalysis

Charles E. Lyman Quantitative X-Ray Microanalysis in the
Environmental SEM

David B. Williams Microbeam Analysis of Metallic Meteorites

Eric Lifshin Pushing the Limits of Spatial
Resolution in X-Ray
Microanalysis

Come and join the celebration Dean Goldstein's contributions to science of
microscopy and educating so many microscopists.

The EAS meeting will be held in the Atlantic City Convention Center,
October 1-4, 2001. For more information contact the EAS Homepage:(
http://www.eas.org).

John A. Reffner, President NYMS



From daemon Mon Sep 3 05:59:20 2001



From: Divakar :      divakar-at-igcar.ernet.in
Date: Mon, 3 Sep 2001 16:16:35 +0530
Subject: [MatSci] [HRTEM] Semper image file created by EMS

Contents Retrieved from Microscopy Listserver Archives
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I have run into a file format problem between Semper for Windows(image
processing software from Synoptics, UK running on Microsoft Windows 98)
and EMS (image simulation software by Prof Stadelmann running on a
Silicon Graphics workstation). The EMS suite of programs for image
simulation has a routine se1 that writes the R type images (created by
the im1 routine, in the present case) in Semper file format which is
essentially a Fortran unformatted output. This file is to be read into
Semper using the unformatted option of the read command. However I get
a

Message: illegal structure for unformatted file
?129: File I/O error 6419 on unit 1 file
'd:\users\divakar\semper\images\rd1082.unf'

error on Semper for Windows and a similar message on Semper for DOS
version 6.4. I would appreciate help / advise from members of this list
in this regard. Is this because the binary code on Unix and DOS
/Windows is different? Or is this a version problem? Does somebody have
software / image file / disc file format details which can be used to
inter-convert between these operating systems and / or hardware?

With Best Regards,
----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----






From daemon Mon Sep 3 06:42:45 2001



From: jesper.v.carstensen-at-risoe.dk
Date: Mon, 03 Sep 2001 13:36:55 +0200
Subject: Electroscan E3 SEM question

Contents Retrieved from Microscopy Listserver Archives
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Dear Gordon,

We have an Electroscan ESEM E3, on which we have had different problems with
the stage. In one case it was necessary to change some cables to fix the
problem, but occasionally the stage won't move in the X-Y directions, and
the problem is solved simply by changing the magnification on the
microscope!! We haven't yet been able to come up with an explanation for
this strange behaviour, but I hope for you, that your problems may be solved
in this simple way.

Kind regards,
Jesper
________________________________
Jesper Vejloe Carstensen
Electron Microscopy & Microanalysis
Risoe National Laboratory, Materials Research Department
P.O. Box 49, DK-4000 Roskilde, DENMARK
Phone: 46 77 57 76, Fax: 46 77 57 58
E-mail: jesper.v.carstensen-at-risoe.dk
Web: www.risoe.dk/afm




-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: 31. august 2001 18:24
To: Microscopy-at-sparc5.microscopy.com


Hello,
I've been going through setting up an Electroscan ESEM E3 in our
laboratory and vacuum problems aside, we now have a stage that doesn't
want to move. Anyone ever come across stage problems and have some
suggestions that I may try for this instrument?

The stage will actually move if you give it a gentle push along a gear
arm, but it often locks up in the Z direction, and sometimes X/Y. Auto
calibrate allows the stage to move in X/Y, but it never calibrates the Z
direction.
Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Mon Sep 3 11:11:34 2001



From: Martin Roe :      Martin.Roe-at-nottingham.ac.uk
Date: Mon, 03 Sep 2001 17:02:31 +0100
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
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Dear fellow list server members,
We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.

Beam Window Cu L Cu K K/L ratio
20 Be 47461 156947 3.3
20 utw 84033 110570 1.3
15 Be 18357 24336 1.3
15 utw 34930 18172 0.5
10 Be 15225 1070 0.07
10 utw 28005 880 0.03

Mant thanks.

Martin Roe
Electron Microscopist
Materials/Engineering Department
Wolfson Building
Nottingham University
University Park
Nottingham
NG7 2RD
tel +44 (0) 115 9513768
tel =44 (0) 115 9513871
fax +44 (0) 115 9513764
email: martin.roe-at-nottingham.ac.uk



From daemon Mon Sep 3 21:10:32 2001



From: Paiboon NUANNIN :      npaiboon-at-ratree.psu.ac.th
Date: Tue, 4 Sep 2001 08:59:13 +0700 (ICT)
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
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Dear Martin

Due to you didn't mention anything about live time, beam
condition, i.e, beam current, beam alignment, working distance and
ect.. so it is quite difficult to suggest anything about your
results. However, it seems likely that at 15kv (Be) and 20k(utw)
are OK. Normally, at 20kV, K/L ratio is close to 1, it depends on
the condition of the detector.


Try Ni K/L to confirm that, K/L ratio would be about 1 at 20 kV.

Regards,

Paiboob Nuannin
Dept of Physics
Faculty of Science
Prince of Songkla University
Hatyai 90110
Thailand





On Mon, 3 Sep 2001, Martin Roe wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear fellow list server members,
} We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
} What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.
}
} Beam Window Cu L Cu K K/L ratio
} 20 Be 47461 156947 3.3
} 20 utw 84033 110570 1.3
} 15 Be 18357 24336 1.3
} 15 utw 34930 18172 0.5
} 10 Be 15225 1070 0.07
} 10 utw 28005 880 0.03
}
} Mant thanks.
}
} Martin Roe
} Electron Microscopist
} Materials/Engineering Department
} Wolfson Building
} Nottingham University
} University Park
} Nottingham
} NG7 2RD
} tel +44 (0) 115 9513768
} tel =44 (0) 115 9513871
} fax +44 (0) 115 9513764
} email: martin.roe-at-nottingham.ac.uk
}
}



From daemon Tue Sep 4 00:37:36 2001



From: marienti-at-tiscalinet.it
Date: Tue, 4 Sep 2001 07:30:38 +0200
Subject: =?iso-8859-1?Q?TEM=20CM10=20Help=21?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers!

This is a request of information about a CM10.

We tried a stretch alignment of the column to verify the basic astigmatism.

In low mag, specially in the central and lower magnification range (between
50 and 250) we see a ?blade? cutting the field of view only without any
condenser aperture inserted.

This blade is the objective aperture support.

I suppose it is a normal thing, but I like to be sure about it.

Is the same on your machine?

If it is so, please let me know.

Thanks a lot!



P.S. Thanks Nestor!



Marco Arienti






__________________________________________________________________
Abbonati a Tiscali!
Con VoceViva puoi anche ascoltare ed inviare email al telefono.
Chiama VoceViva all' 892 800 http://voceviva.tiscali.it






From daemon Tue Sep 4 00:39:20 2001



From: Marco Arienti :      marienti-at-tiscalinet.it
Date: Tue, 4 Sep 2001 07:32:34 +0200
Subject: TEM CM10 Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers!

This is a request of information about a CM10.

We tried a stretch alignment of the column to verify the basic astigmatism.

In low mag, specially in the central and lower magnification range (between
50 and 250) we see a "blade" cutting the field of view only without any
condenser aperture inserted.

This blade is the objective aperture support.

I suppose it is a normal thing, but I like to be sure about it.

Is the same on your machine?

If it is so, please let me know.

Thanks a lot!



P.S. Thanks Nestor!



Marco Arienti







From daemon Tue Sep 4 01:08:18 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 03 Sep 2001 23:05:07 -0700
Subject: Update Zeiss LM Service....

Contents Retrieved from Microscopy Listserver Archives
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Regarding my recent experiences with my used Axioplan, I
consider the issue to be closed. Zeiss sent a factory technician
to my site and corrected the problem at no charge.

gary g.



From daemon Tue Sep 4 02:15:27 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 4 Sep 2001 08:16:15 +0100 (GMT Daylight Time)
Subject: Re: EDS Detector sensitivity problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Martin,

I have little experience with SEM and EDX detector
performance, however, I have used a range of Be, thin
window and windowless detectors on TEMs and there are a
couple of comments I would like to make.

If the degradation in performance occurred directly after
the oil backstreaming then I agree that oil is quite likely
to be the cause but otherwise it is quite possibly ice on
the crystal.

Has the detector liquid nitrogen always been kept well
filled? If the internal 'cryo pump' starts to warm up then
you could get icing on the crystal.

Why not condition the detector anyway? Certainly on my
Oxford Instruments (ex Link) detectors it is easy to do and
does not seem to degrade the performance at all. I
regularly condition the windowless detector on my JEOL2010
TEM (about every 6 months) to get the light element
performace back. However, I have not had to condition the
SATW detector (on another TEM) in over two years so I guess
the water comes from the microscope and not the detector.

Regards,
Ron

On Mon, 03 Sep 2001 17:02:31 +0100 Martin Roe
{Martin.Roe-at-nottingham.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear fellow list server members,
} We have a Si(Li) detector with Be/UT window attached to a SEM. Some time before I started my job here there was a vacuum fault where pump oil backstreamed into the SEM column -my understanding is that the Be window was in position at the time. We are now seeing low counts for light elements when used in thin window mode and quantitative data reflect this, even though the oil streamed in when the Be window was being used. The manufacturer has suggested the likely source is ice on the crystal (from figures below). This problem I am assured only started after the vacuum/oil problem which indicates oil is the likely problem. I include K/L line data collected from a copper sample at 10, 15, 20 kV with both the Be and UT windows in place. The counts represent the peak windows/ROI of the K and L lines.
} What do you make of these figures below? As I have no reference data or anything to compare with. I would appreciate any input or suggestions you have.
}
} Beam Window Cu L Cu K K/L ratio
} 20 Be 47461 156947 3.3
} 20 utw 84033 110570 1.3
} 15 Be 18357 24336 1.3
} 15 utw 34930 18172 0.5
} 10 Be 15225 1070 0.07
} 10 utw 28005 880 0.03
}
} Mant thanks.
}
} Martin Roe
} Electron Microscopist
} Materials/Engineering Department
} Wolfson Building
} Nottingham University
} University Park
} Nottingham
} NG7 2RD
} tel +44 (0) 115 9513768
} tel =44 (0) 115 9513871
} fax +44 (0) 115 9513764
} email: martin.roe-at-nottingham.ac.uk
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Sep 4 06:52:39 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Tue, 04 Sep 2001 08:25:05 -0700
Subject: Re: YAG and YAP scintillators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon-

I'll second what Jesper says. Our E3 also occasionally does the
"lock-up" thing but can always be freed up simply by changing the
magnification. Why this should fix it is, I think, one of the great
mysteries of the universe. We've also occasionally experienced slow movement
of the stage in the XY direction. This can make acquiring a digital image a
real problem at times. Usually you can stop this drift by changing the
pressure in the chamber.....Great Mystery of the Universe #2.....

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.Berkeley.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 31, 2001 1:23 PM


Charles Garber in his recent posting wrote:
} Not all YAG's and
} YAP's are created equal. So when making comparisons, and statements about
} their scintillators, it would be helpful for one to state the brand of their
} scintillator crystal. These crystals are certainly not a generic entity
} where all are the same irrespective of the source.

I am a bit puzzled by this. I was under the impression that all of the
YAG and YAP scintillators offered for sale in this country originate
from the same ultimate supplier in Europe. Has this changed? Or
perhaps there are different grades of crystal?

Fred Schamber
Aspex LLC


"Garber, Charles A." wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Earl Weltmer and Brad Johnson wrote:
} ============================================================
} } Hello,
} } I need to replace the SE scintillator on my SEM (JEOL 5900), and I was
} } considering using a YAP scintillator instead of the typical phosphorous
} } scintillator. Does anyone have any experience or suggestions on this?
} ++++Brad Johnson
}
} About ten years ago I ran some tests for signal on several scintillator
} types: YAP, YAG, standard aluminum coated scintillator & "uncoated"
} scintillator.
} The result at that time were surprising.
}
} Using a JEOL 35C SEM & running the same specimen current & PMT voltage with
} each scintillator the best signal was given by the original aluminum coated
} scintillator.
}
} The second best was the "uncoated" scintillator & it was only 80% of the
} signal the standard aluminum coated scintillator.
} The YAP & YAG gave only 60% of the standard aluminum scintillator.
}
} I was very disappointed as the YAP & YAG were significantly more than the
} standard scintillator.
} The conclusion that I came to was that the YAP & YAG would probably give
} more longevity & could justify their extra cost but they were not fit for
} high resolution. The YAP & YAG would probably be suitable for probe work.
}
} Keep in mind this experiment was done about ten years ago. Perhaps the
} crystals have improved in recent years & the data may need a re-evaluation.
} I am sure that there is someone who would be willing to criticize my results
} (maybe in Northern Calif.?) but this the data that I received at the time.
} +++++ Earl Weltmer
} ============================================================
} We have offered P-47, YAG and YAP scintillators for SEM applications for
} more then twenty years. Earl is correct that the P-47 performance, **but
} when first installed**, does probably give a superior performance. However
} , not all YAG and YAP crystals come out of the "same cookie cutter." From
} what we have been able to deduce, a high quality YAG or YAP single crystal
} scintillator will perform so close to that of the P-47 that one usually has
} difficulty detecting the difference. However, as everyone knows, the P-47
} once installed, in terms of performance, goes only down. The only question
} is how fast it will go down. And that rate of deterioration of performance
} depends among other things on the type of work being done. For example, the
} higher x-ray fluxes associated with EDS (and especially WDS) tend to cause a
} more rapid deterioration.
}
} Also, if Earl's experiment was done as described, and if a YAG was tested
} against P-47 but using the same PMT, then one would expect to find an
} inferior performance, because the PMT that is optimum for P47 is not the
} same one that would have been optimum for YAG. Now this might be less of a
} problem today than it would have been ten years ago, but if the PMT was not
} changed, then the right test (IMHO) was not the one being performed. To
} make the comparison, one would have had to have taken out the S11 style and
} replaced it with an S20 PMT. This point is further explained on our website
} URL
} http://www.2spi.com/catalog/scintill/spi-yag.html
}
} When a YAG or YAP scintillator is installed, its performance level is
} constant and does not deteriorate. So while the comparison might suggest
} some superiority at the very beginning, in most instances that we have seen,
} or have been led to believe, it is not long that the SEM running on YAG or
} YAP in general, is operating at a higher level of performance. And the SEM
} is not subjected to the downtime associated with the need to change a P-47
} powder scintillator from time to time.
}
} For those operating at low KV in the BSE mode (and using YAG or YAP), and
} high magnifications, the image is clearly less noisy. A collection of ten
} different references from different laboratories around the world, which
} have been collected on URL
} http://www.2spi.com/catalog/scintill/sem-tem.html
} at least to some, pretty much document the superiority of the single crystal
} scintillators over the powder scintillators. I guess one could always try
} to do more studies, but anyone that is familiar with these publications
} finds their conclusions pretty persuasive.
}
} We have generally recommended that the SPI single crystal scintillators
} offered many advantages over the more traditional P-47 scintillator. We
} have been under the impression that those who have made the switch have been
} pretty happy with their results, though we do hear from time to time of
} someone who would not agree with that statement. Of course, the performance
} is related to the nature of the doping of the crystal, and as I said, not
} all these crystals come out of the same cookie cutter. Not all YAG's and
} YAP's are created equal. So when making comparisons, and statements about
} their scintillators, it would be helpful for one to state the brand of their
} scintillator crystal. These crystals are certainly not a generic entity
} where all are the same irrespective of the source.
}
} Disclaimer: SPI Supplies is a major supplier of scintillators for SEM
} applications including P-47's, YAGs and YAPs.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================


From daemon Tue Sep 4 08:45:16 2001



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Tue, 04 Sep 2001 08:45:20 -0700
Subject: RE: FE-SEM: Historical Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim

I think you mean FE-STEMs not FE-SEMs.

The first VG HB5 FE-STEM was delivered in 1973 to University of London.

Both AEI and Seimens got into dedicated STEM shortly before they pulled out
of the EM market in the early 70's. VG was able to attract a number of the
main characters who had worked at AEI to form the core of the VG
Microscopes unit.

The first electron microscope VG produced was a W thermal sourced SEM
(Miniscan) for which they were awarded the Queens award for technological
innovation in 1970. They then went on to produce the HB5 High Vacuum
FE-STEM and HB50 High Vacuum FE-SEM. All of the latter were sold with
Auger spectrometers as a Scanning Auger Microprobe.

Regards

Alan

At 09:55 AM 8/31/2001 +1000, Jim at Proscitech wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Tue Sep 4 09:38:53 2001



From: Bruce Ingber :      bingber-at-srrc.ars.usda.gov
Date: Tue, 04 Sep 2001 09:31:04 -0500
Subject: Electroscan E3 SEM question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Bruce

This is normal!

On both the E3 and 2020 the stage movement with the Joy Stick is linked to
magnification. The higher the mag the slower the stage speed. Above about
8000 X the stage motors are disabled and beam shift is active with the Joy
Stick.

The apparent movement of the image should remain the same at all mags. If
the mag is above about 8KX then the amount of image movement is limited by
the beam shift. To continue searching the magnification has to be lowered.

For ElectroScan ESEM questions (E2,E3,Explorer, 2010 or 2020) world wide
contact me directly at dsimpson-at-ma.feico.com
For XL ESEM (XLFEG ESEM, XLLab6 ESEM or XLTMP ESEM in North America contact
me directly at dsimpson-at-ma.feico.com
For XL ESEM (XLFEG ESEM, XLLab6 ESEM or XLTMP ESEM for the rest of the world
contact their local Service group.


Regards
Derek Simpson
ESEM Technical Support Manager
FEI Company
One Corporation Way #2
Peabody, MA 01960
E-mail: dsimpson-at-feico.com
Voice: 1.978.538.6700
Fax: 1.978.531.9648


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Gordon,

We have an Electroscan ESEM E3, on which we have had different problems with
the stage. In one case it was necessary to change some cables to fix the
problem, but occasionally the stage won't move in the X-Y directions, and
the problem is solved simply by changing the magnification on the
microscope!! We haven't yet been able to come up with an explanation for
this strange behaviour, but I hope for you, that your problems may be solved
in this simple way.

Kind regards,
Jesper
________________________________
Jesper Vejloe Carstensen
Electron Microscopy & Microanalysis
Risoe National Laboratory, Materials Research Department
P.O. Box 49, DK-4000 Roskilde, DENMARK
Phone: 46 77 57 76, Fax: 46 77 57 58
E-mail: jesper.v.carstensen-at-risoe.dk
Web: www.risoe.dk/afm


-----Original Message-----
} From: Gordon Vrololjak [mailto:gvrdolja-at-nature.Berkeley.EDU]
Sent: 31. August 2001 18:24
To: Microscopy-at-sparc5.microscopy.com


Hello,
I've been going through setting up an Electroscan ESEM E3 in our
laboratory and vacuum problems aside, we now have a stage that doesn't
want to move. Anyone ever come across stage problems and have some
suggestions that I may try for this instrument?

The stage will actually move if you give it a gentle push along a gear
arm, but it often locks up in the Z direction, and sometimes X/Y. Auto
calibrate allows the stage to move in X/Y, but it never calibrates the Z
direction.
Thanks.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Tue Sep 4 10:36:09 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Tue, 4 Sep 2001 11:30:30 -0400
Subject: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krassimer,

An alternative you may want to consider is the Polaron E6700 Evaporator,
manufactured by Thermo VG Scientific. We are their distribution and service
agent in the U.S. and would be happy to supply you with more information.

Best regards,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
mnesta-at-ebsciences.com
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: K.N. Bozhilov [mailto:bozhilov-at-citrus.ucr.edu]
Sent: Friday, August 31, 2001 12:27 PM
To: microscopy-at-sparc5.microscopy.com


Hi All,

I would greatly appreciate if you could share your opinion about performance
and reliability of the Bench Top Turbo III vacuum evaporator from Denton
Vacuum, also alternative suggestions are more than welcome. We are looking
currently to purchase a vacuum evaporator for general EM preparation capable
of carbon and metal evaporation, glow discharge and thickness control.
Cleanliness of the vacuum is our main concern and especially the fact that
the Bench Top model does not have LN trap.

Thank you,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

Tel 909 787 2998
Fax 909 787 4324





From daemon Tue Sep 4 11:31:03 2001



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 4 Sep 2001 12:24:16 -0400
Subject: Oil diffusion pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List,

We are parting with a used oil diffusion pump (w/ LN2 Trap) that has approx.
2 years wear and tear. The pump has been in storage for the last 10 years.
The pump was manufactured by DAIA vacuum Engineering corp, model # DPF4Zs.
The mounting flange is approx. 7.25 inches with an 8 hole bolt pattern. It
was removed from a Hitachi S-4000 FESEM for replacement by a cleaner turbo
model. If you are interested please contact me off-line. The pump is free
but you must assume all shipping costs. Thanks, jr

Particulars: 570 l/sec
100V / 500W
Pressure = 10 -7torr
Oil capacity 150cc


John A. Robson
} _____________________________________________________
}
} Boehringer Ingelheim Pharmaceuticals, Inc.
} Research and Development
} 900 Ridgebury Road / P. O. Box 368
} Ridgefield, CT 06877-0368
}
} phone: 203.798.5640
} fax: 203.798.5698
} email: jrobson-at-rdg.boehringer-ingelheim.com
}
}
}


From daemon Tue Sep 4 11:36:02 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 04 Sep 2001 11:30:35 -0500
Subject: Re: [MatSci] [HRTEM] Semper image file created by EMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I will hazard one guess being unfamiliar with either of the programs or
formats that you describe. I suspect that there is a difference in how the
two systems format text files. Under DOS and Windows, text lines normally
end with a carriage return and line feed characters, ASCII codes 13 and 10.
Under UNIX, lines often end with one or the other, but not both; I think
carriage return is the norm.

I know that WS-FTP has an option for converting text files to the proper
standard when transferring between the two different platforms. It might be
able to help, but you will need to tell it that the file are text. It
ordinarily runs in auto-detect mode and determines file type based on the
extension. Unknown extensions default to binary format. Presuming you have
to ship your files anyway, this might eliminate the need for a translator
program.

There are also a number of utilities around that could give you a look at
the binary codes for your file to see what characters are being used to
indicate the end of the line. I have some I could recommend on the PC side
if you don't already have one. They are relatively available on the
shareware sites and could help verify what I have said. Some even serve as
editors so you could make changes to one file manually to see if this is
the source of your problem.

Warren

At 04:16 PM 9/3/2001 +0530, you wrote:

} I have run into a file format problem between Semper for Windows(image
} processing software from Synoptics, UK running on Microsoft Windows 98)
} and EMS (image simulation software by Prof Stadelmann running on a
} Silicon Graphics workstation). The EMS suite of programs for image
} simulation has a routine se1 that writes the R type images (created by
} the im1 routine, in the present case) in Semper file format which is
} essentially a Fortran unformatted output. This file is to be read into
} Semper using the unformatted option of the read command. However I get
} a
}
} Message: illegal structure for unformatted file
} ?129: File I/O error 6419 on unit 1 file
} 'd:\users\divakar\semper\images\rd1082.unf'
}
} error on Semper for Windows and a similar message on Semper for DOS
} version 6.4. I would appreciate help / advise from members of this list
} in this regard. Is this because the binary code on Unix and DOS
} /Windows is different? Or is this a version problem? Does somebody have
} software / image file / disc file format details which can be used to
} inter-convert between these operating systems and / or hardware?
}
} With Best Regards,
} ----
} Divakar R
} Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
} Kalpakkam 603102, India
} ----

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Tue Sep 4 12:00:14 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Tue, 04 Sep 2001 12:54:41 -0400
Subject: Re: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Krassimir,

It is Ladd's belief that the ideal vacuum evaporator should include a
mechanical and a diffusion pump to get you to the 10(-7) range and
should have a LN trap. The evaporation system should have a protective
shield for cleanliness.
If you are doing chromium evaporation then a turbo pump would be
required.

John Arnott

Disclaimer: Ladd Research manufactures and sells vacuum evaporation
systems.
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955


K.N. Bozhilov wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Hi All,
}
} I would greatly appreciate if you could share your opinion about performance
} and reliability of the Bench Top Turbo III vacuum evaporator from Denton
} Vacuum, also alternative suggestions are more than welcome. We are looking
} currently to purchase a vacuum evaporator for general EM preparation capable
} of carbon and metal evaporation, glow discharge and thickness control.
} Cleanliness of the vacuum is our main concern and especially the fact that
} the Bench Top model does not have LN trap.
}
} Thank you,
}
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} Tel 909 787 2998
} Fax 909 787 4324


From daemon Tue Sep 4 12:00:18 2001



From: Joyce Craig :      bafpjec-at-csu.edu
Date: Tue, 04 Sep 2001 11:55:20 -0500
Subject: knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is possible to break the 8mm glass, but it can be very frustrating.
If the users want wider knives, try to talk them into purchasing a
diamond knife for thick sections. It is less expensive than diamonds
for thin sectioning, and I think it is a good buy because of time saved
making and changing knives and attaching water catchers.
The only downside is that once there is a nick in the edge it is there
until resharpening time.



From daemon Tue Sep 4 12:58:01 2001



From: =?iso-8859-1?Q?Patr=EDcia?= Reis :      pmreis-at-morango.esb.ucp.pt
Date: Tue, 4 Sep 2001 12:52:58 -0500
Subject: buffer preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi everyone,

I would like to prepare 0.05 M Cacodylate buffer pH 7.4, but do not
have the recipe and protocol necessary for its preparation.
Would it be possible to help me out?

Thanks in advance.

Patrícia Reis
____________________________
Escola Superior Biotecnologia
Universidade Católica Portuguesa
Rua Dr. António Bernardino Almeida
4200-072 Porto
PORTUGAL
Tel.: 225580044
Fax.: 225090351
e.mail: {mailto:pmreis-at-mail.esb.ucp.pt} pmreis-at-mail.esb.ucp.pt


From daemon Tue Sep 4 13:42:26 2001



From: Judy Trogadis :      TrogadisJ-at-smh.toronto.on.ca
Date: Tue, 04 Sep 2001 09:13:22 -0400
Subject: imaging courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists:

I would like to attend a course on microscopy and imaging techniques which would include the latest developments in this field. Apart from the ones at MBL, Cold Spring Harbor and Jim Pawley's course in June, does anyone know of others. Being quite keen, I would prefer something this winter but any suggestions would be highly appreciated.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Tue Sep 4 14:49:06 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 04 Sep 2001 12:40:36 -0700
Subject: Minot Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
A colleague recently inherited an IEC Minot Rotary Microtome. Does anyone
have a manual or parts? It is missing the speciman holder, mounting bar
for knife height adjustment and means of measuring knife angle.

Thanks in advance,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************




From daemon Tue Sep 4 18:56:38 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 4 Sep 2001 17:36:41 -0600
Subject: Re: [MatSci] [HRTEM] Semper image file created by EMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could also be a processor related problem. There is a difference in the way
the Intel and Motorola processors store information. One of the two writes
the LSB (least significant byte) first, the other the MSB (most significant
byte). The best bet would probably be to use a format that can be read by
both. TIF, for example, can be normally read by both as the file itself
contains information about the byte order.

Can you transform your images into TIF?

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Warren E Straszheim [mailto:wesaia-at-iastate.edu]
Sent: Tuesday, September 04, 2001 10:31 AM
To: divakar-at-igcar.ernet.in
Cc: Microscopy-at-sparc5.microscopy.com


I will hazard one guess being unfamiliar with either of the programs or
formats that you describe. I suspect that there is a difference in how the
two systems format text files. Under DOS and Windows, text lines normally
end with a carriage return and line feed characters, ASCII codes 13 and 10.
Under UNIX, lines often end with one or the other, but not both; I think
carriage return is the norm.

I know that WS-FTP has an option for converting text files to the proper
standard when transferring between the two different platforms. It might be
able to help, but you will need to tell it that the file are text. It
ordinarily runs in auto-detect mode and determines file type based on the
extension. Unknown extensions default to binary format. Presuming you have
to ship your files anyway, this might eliminate the need for a translator
program.

There are also a number of utilities around that could give you a look at
the binary codes for your file to see what characters are being used to
indicate the end of the line. I have some I could recommend on the PC side
if you don't already have one. They are relatively available on the
shareware sites and could help verify what I have said. Some even serve as
editors so you could make changes to one file manually to see if this is
the source of your problem.

Warren

At 04:16 PM 9/3/2001 +0530, you wrote:

} I have run into a file format problem between Semper for Windows(image
} processing software from Synoptics, UK running on Microsoft Windows 98)
} and EMS (image simulation software by Prof Stadelmann running on a
} Silicon Graphics workstation). The EMS suite of programs for image
} simulation has a routine se1 that writes the R type images (created by
} the im1 routine, in the present case) in Semper file format which is
} essentially a Fortran unformatted output. This file is to be read into
} Semper using the unformatted option of the read command. However I get
} a
}
} Message: illegal structure for unformatted file
} ?129: File I/O error 6419 on unit 1 file
} 'd:\users\divakar\semper\images\rd1082.unf'
}
} error on Semper for Windows and a similar message on Semper for DOS
} version 6.4. I would appreciate help / advise from members of this list
} in this regard. Is this because the binary code on Unix and DOS
} /Windows is different? Or is this a version problem? Does somebody have
} software / image file / disc file format details which can be used to
} inter-convert between these operating systems and / or hardware?
}
} With Best Regards,
} ----
} Divakar R
} Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
} Kalpakkam 603102, India
} ----

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking



From daemon Tue Sep 4 19:46:29 2001



From: Long Miao :      lmiao-at-bio.fsu.edu
Date: Tue, 04 Sep 2001 20:41:52 -0400
Subject: How to refill the micromanipulator system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear lister?
Our Narishige WR-90 micromanipulator does not work properly because the
the water inside the system has leaked out slowly. Does anyone in the list
know how to refill it? Any suggestion is highly appreciated.
Thanks,
Long
-----------------------------------
Miao, Long
Dept of Biological Science
334 Bio. Unit1
Florida State University
Tallahassee, FL32306-4370
email: lmiao-at-bio.fsu.edu
Voice: (850)644-9817
FAX : (850)644-0481

-----------------------------------



From daemon Tue Sep 4 20:28:23 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 4 Sep 2001 20:23:07 -0500
Subject: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
If any of you have removed a jammed film cassette from a JEOL
1200EXII, I
would like to know how you dislodged it. We will eventually have a service
technician to help but I cannot figure out how to remove the plate. I did
manage to remove the film and yes the plate was inserted right side up.
Thanks in advance.
Rosemary


From daemon Tue Sep 4 22:49:37 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Tue, 4 Sep 2001 23:42:32 -0400
Subject: imaging courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A one day short course on "Digital Image Capture and Management in Light
Microscopy" by Dr. Mary McCann & Dr. John McCann is being offered on Sunday
September 30,2001 at the Eastern Analytical Symposium and Exhbition in
Atlantic City, NJ.

Don O'Leary

-----Original Message-----
} From: Judy Trogadis [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Tuesday, September 04, 2001 9:13 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: Judy Trogadis


Fellow Microscopists:

I would like to attend a course on microscopy and imaging techniques which
would include the latest developments in this field. Apart from the ones at
MBL, Cold Spring Harbor and Jim Pawley's course in June, does anyone know of
others. Being quite keen, I would prefer something this winter but any
suggestions would be highly appreciated.

Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca






From daemon Wed Sep 5 02:42:47 2001



From: :      mcmouldk-at-203.193.14.120
Date: Wed, 5 Sep 2001 15:33:33 +0800
Subject: JEOL Camera Jams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rosemary,

I have experience many jams. Generally occurring with old age. Anyway,
JEOL camera mechanisms are wonderful mechanical
beasts. Two pushing nails in the mechanism catch the plates. If you look
at the plates, on one side there are two small
rectangles, which are cut out. The nails use these to capture the plates
and push them through the mechanism. These
nails, together with the plates wear down and eventually sometimes they
miss and do not mate. Generally jamming in the
open slot where the plates come out of the box.

So, to dislodge a jam there are several solutions:

1) Waggle the mechanisms in the hope it clears - not a good choice.
2) Make a piece of stiff plastic sheet the width of a plate and the length
around 15". Try to insert the sheet above
the canisters but below the top mechanisms so as to lift the nails free of
the canisters. You can see the nails when you
look into the camera above the boxes on both sides. They look like small
arms with a piece of copper alloy on the end.
Pull camera out if nails are free.
3) Remove the whole camera mechanism. Simply remove the bolts from
below the camera chamber (6 I think), and then pull
the camera mechanism out in one piece. The jam can then be freed by
rotating the mechanism a wee bit.

If you have frequent jams, consider replacing the pushing nails (first) then the
plates. Also a good idea to replace
the Teflon sheet bearings, but get a JEOL engineer to do this otherwise the
position of the mechanism could be lost.

Good luck,

Keith.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From daemon Wed Sep 5 06:26:35 2001



From: Alan Davis :      adavis-at-saipan.com
Date: Wed, 5 Sep 2001 21:16:23 +1000
Subject: Camera Lucida drawing tube (Zeiss) questions

Contents Retrieved from Microscopy Listserver Archives
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I have gotten ahold of a Zeiss camera lucida drawing tube. It has a mirror at an angle that allows one to draw underneath the inclined eye tube. Works for a Zeiss Standard 16. I am getting the hang of it a bit. However, I have at least one question:

What is the collar for (referred to as "ring clamp" below)? It is somewhat eccentric, but I cannot imagine any purpose for it. The device doesn't fit over it, but nearly does. It *is* useful for attaching it to a wider eyepiece tube.

This plastic collar fits snugly inside the eyepiece tube of an Olympus SZH; this is good. Also, it fits over the end of the standard 16 eye tube, with a barrier that doesn't allow it to slip down over the tube. i have some documentation for a similar drawing tube, but there is a slight difference in the picture (there is a knurled knob at the point where the mirror/prism fits over the eyepiece. Mine doesn't have that, it fits snug). Here is the relevant section from the documentation for the pictured device:

1. Focus microscope.

2. Remove eyepiece and slip ring clamp on to tube. Replace eyepiece
and fix with ring clamp. [I referred to the "ring clamp" as a collar.]

3. Clamp drawing prism on the eyepiece. [I assume this refers to the knurled
screw that isn't present on my device---mine doesn't need to be clamped on.]



} From reading the description, though, I don't know what it is for. Is it a spacer to move the eyepiece up higher? Which Zeiss is it really designed for?

Also, I would greatly appreciate any tips. The tips I found in MICSCAPE were helpful. Can I initiate some correspondence with someone who has some experience with one of these fantastic devices?

Alan Davis
Marianas High School

--
adavis-at-saipan.com 1-670-235-6580
Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI

I have steadily endeavored to keep my mind free, so as to give up any
hypothesis, however much beloved -- and I cannot resist forming one
on every subject -- as soon as facts are shown to be opposed to it.
-- Charles Darwin (1809-1882)






From daemon Wed Sep 5 07:33:24 2001



From: JHumenansky-at-phi.com
Date: Wed, 5 Sep 2001 07:16:26 -0500
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Rosemary, I believe that the JEOL 1200 camera chamber is the same as the
JEOL 100CX. You can slide out the film tray by making two metal strips
measuring about 1" to 2" wide and about 10" to 12" long. Bend one end of
each strip in the shape of an allen wrench to use as a sort of handle.
These metal strips are used to raise up the two little arms that are
locking up the film tray. While sitting on the floor so that the open
camera chamber is at eye level and using a bright light you can look into
the top area of the camera chamber just above where the film boxes are
located. On each side and above the film boxes you will see these arms
sticking down. You can use the metal strips to release these arms be
sliding the metal strips straight in.
Be sure that the film tray is all the way in so that there is no force on
these arms holding them down. They are spring loaded and should raise up
with only gently pushing on the metal strips. Be careful and do not apply
a lot of force. You should be able to see these arms raise when you push
in the metal strip. When both arms are raised the drawer should pull out
easily.
Again, don't use force. Hope that this helps.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Wed Sep 5 08:15:36 2001



From: Patton Steve T Contr AFRL/MLBT :      Steve.Patton-at-wpafb.af.mil
Date: Wed, 5 Sep 2001 09:08:55 -0400
Subject: position opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all. Note the following opportunity.

Steve Patton

Systran Federal Corporation in Dayton, Ohio, is managing an "on-site" Contract for the Materials and Manufacturing Directorate (ML), Air Force Research Laboratory (AFRL), at Wright Patterson Air Force Base, in Dayton, Ohio. Candidates are sought for a research position at AFRL/ML, and this position entails research and development on understanding surface forces and surface chemistry of MEMS devices and MEMS materials:

a) study surface forces of MEMS using scanning probe microscopes and special adhesion/friction testers as a function of environment (humidity, temperature, vacuum); develop an understanding of how surface forces, and hence friction and wear may be controlled using surface treatments including monolayers and solid coatings
b) study surface chemistry, tribochemistry, and coating microstructure to elucidate the mechanisms controlling friction and wear in MEMS systems under extreme environments
c) research lubricant transport behavior and dynamics in MEMS systems.

The successful candidate will have knowledge/experience of MEMS devices, surface chemistry, surface forces and tribology. Expertise in surface chemical probes such as scanning Auger microscopy, X-ray photoelectron spectroscopy, FTIR microscopy / grazing angle FTIR, micro Raman, and SEM/EDS are highly desirable. The candidate will use apparatus to study surface forces at the nano scale (scanning probe microscopes, special adhesion testers, and nano/micro friction measurement devices). Working knowledge of monolayer and solid coatings is also highly desirable. The focus of the research effort is to understand the chemistry and physics controlling friction and wear and to use that understanding to develop effective technologies leading to reliable MEMS operation in extreme environments. Analytical instrumentation is currently available, but the successful candidate must be capable of modifying instrumentation as part of the research program.

The advertised position is a full-time job with complete benefits (paid vacation, health and dental coverage, 401K, etc.). Interested candidates should respond to this advertisement by sending their resumes along with a listing of technical publications to the contact person listed below.

Dr. V. ("Nagu") Nagarajan
V.P. & Program Manager
Systran Federal Corporation
4027 Colonel Glenn Highway, Suite 210
Dayton, OH 45431-1672

Tel: (937)-429-9008, ext. 353
Fax: (937)-429-9461

E-mail: nagu-at-systranfederal.com
Web: http://www.systranfederal.com




From daemon Wed Sep 5 09:26:06 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Wed, 5 Sep 2001 09:16:48 -0500
Subject: carbon sticky tab arrays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

We have an SEM application which requires an array of samples to be loaded
into the microscope. For producing the array, we need to affix sticky tabs
to an aluminum block (48 tabs in a 6 x 8 arrangement on a block about 3
inches by 4 inches).

Presently, we are producing these arrays by hand, but it would be much more
efficient to produce the arrays in this form by simply pressing a sheet onto
the block, then peel it off to give us all of the 48 tabs properly located
on the plate in one shot.

Does anyone have a name of the manufacturer(s) of carbon sticky tabs, so we
can contact them about this. Our supplier is not willing to provide this
information.

Thank You,

Wharton Sinkler
UOP LLC
Des Plaines, IL



From daemon Wed Sep 5 12:14:20 2001



From: Axel Blau :      axel-at-caltech.edu
Date: Wed, 5 Sep 2001 10:03:28 -0700
Subject: LOMO water-immersion/dipping lenses for 160 mm-corrected scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On our search for high N.A. water-dipping/immersion objectives for 160
mm-corrected microscopes we came across some lenses made by a Russian
company called 'LOMO Optics' (www.lomooptics.com), which we tried out on our
Nikon Optiphot 2 two-photon scope, and on a Nikon Measurescope MM-11 (bottom
illumination). Their performance is quite astonishing. And since we had to
look for quite a while to find such lenses, we wanted to let the rest of the
community know that you do not have to give away your old 160 mm-corrected
scope yet. Prices are quite reasonable too. At LOMO they are willing to let
you try out the lenses for yourself. But do not be surprised to get a phone
call every day during that time. (And do not forget to negotiate!)

[1] Type EAF-A30-1, TT079, 30x water-immersion, N.A. 0.9, w.d. 1.3 mm, 0.17
mm coverslip correction (performance does not seem to suffer without
coverslip), built-in iris, outer diameter: 0.545" at the tip for about 0.2",
and 0.825" thereafter (the objective has a cap of that diameter, which (at
least in our case) could be removed, giving an even smaller outer diameter
of 0.710"), overall length when installed: ~1.050".

[2] Type 96001, 70x water-dipping (apparently no coverslip correction).,
N.A. 1.23, w.d. 220 µm, outer diameter -at- the tip: 0.426" to 0.585" conical
for about 0.2", 0.800" above,overall length when installed: 1.26".

[3] Type 83127, 85x water-immersion., N.A. 1.0, w.d. between 200 and 230 µm
(depending on the coverslip correction setting, which ranges from 100 µm to
200 µm), outer diameter -at- the tip: 0.733" for about 0.2", 0.825" above,
overall length when installed: 1.26".

[4] Apparently they also sell a 100x water-immersion lens by now, which we
have not seen yet.

With best regards,

Axel

__________________________________

Axel Blau
California Institute of Technology
Division of Biology 156-29
1200 E. California Blvd.
Pasadena, CA 91125

(626) 395-6787 phone
(626) 564-8709 fax

http://wecoyote.caltech.edu/~axelb/
http://www.caltech.edu/~pinelab/pinelab.html



From daemon Wed Sep 5 13:00:22 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 5 Sep 2001 13:50:41 -0400
Subject: carbon sticky tab arrays

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was at a talk where the carbon sticky dots were first presented. I wish I could remember who was speaking. I know that SPI has the exclusive distribution rights to them. I believe that they were developed by a group at Dupont. I would check the literature and see if they published. Try back issues of the meeting abstracts. I think that the talk that I went to was about 10 years ago.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Wednesday, September 05, 2001 10:17 AM
To: 'Microscopy-at-sparc5.microscopy.com'
Cc: Kuechl, Dorothy



Dear Listers,

We have an SEM application which requires an array of samples to be loaded
into the microscope. For producing the array, we need to affix sticky tabs
to an aluminum block (48 tabs in a 6 x 8 arrangement on a block about 3
inches by 4 inches).

Presently, we are producing these arrays by hand, but it would be much more
efficient to produce the arrays in this form by simply pressing a sheet onto
the block, then peel it off to give us all of the 48 tabs properly located
on the plate in one shot.

Does anyone have a name of the manufacturer(s) of carbon sticky tabs, so we
can contact them about this. Our supplier is not willing to provide this
information.

Thank You,

Wharton Sinkler
UOP LLC
Des Plaines, IL



From daemon Wed Sep 5 14:02:17 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 5 Sep 2001 14:44:36 -0400 (EDT)
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can't help you get it out, but just wanted to warn you that it might be
possible for you to get your fingers crushed or severed if you're fishing
around in the chamber and the jam suddenly frees itself. BE CAREFUL.
I'm not sure if this is the case with your model, but it was on an older
JEOL that we had. I'd wait for the tech or at least get his advice
before reaching in.

We had some that would get caught, and the only thing to do with them
after they were freed was to toss them. Don't try to straighten them.

Sara

On Tue, 4 Sep 2001, Rosemary Walsh wrote:

} Date: Tue, 4 Sep 2001 20:23:07 -0500
} From: Rosemary Walsh {rw9-at-psu.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: removing jammed TEM film cassettes
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} If any of you have removed a jammed film cassette from a JEOL
} 1200EXII, I
} would like to know how you dislodged it. We will eventually have a service
} technician to help but I cannot figure out how to remove the plate. I did
} manage to remove the film and yes the plate was inserted right side up.
} Thanks in advance.
} Rosemary
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Sep 5 14:22:08 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 5 Sep 2001 15:22:45 -0400
Subject: RE: Plate Jams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The jamming of the plate drive mechanisms in electron microscopes is
nothing new. I remember back in the 1960s when I was using our two
JEM-6A TEMs in my classes on electron Microscopy. With an average
enrollment of 25 to 30 students per term, coming from all disciplins
across campus, and having all degrees of mechanical aptitude, plate
jams were not an uncommon event. The easiest way to clear up a really
bad plate jam in the JEM-6As was to reach inside the plate chamber
and wiggle the plate carrier around a bit until things worked loose.
Unfortunately, however, the chamber opening was too small for my
hands to get in deep enough to take care of a bad jam, and so on
several occasions I had to enlist the help of my 5-year-old son, who
eventually became quite expert at taking care of the problem. Times
do change, but digital cameras have their problems too!

Good luck, and keep smiling,
WCB
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Sep 5 14:48:27 2001



From: Paul.Nolan-at-alcan.com
Date: Wed, 5 Sep 2001 14:42:43 -0500
Subject: Vibrating Engraver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know where I could purchase a battery powered vibrating
engraving tool?
We have the corded type but I need a portable one to use out on a factory
floor.
Vendor replies welcome


Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com


From daemon Wed Sep 5 14:48:27 2001



From: =?iso-8859-1?Q?Patr=EDcia?= Reis :      pmreis-at-morango.esb.ucp.pt
Date: Wed, 5 Sep 2001 14:43:48 -0500
Subject: about working with fixatives

Contents Retrieved from Microscopy Listserver Archives
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Hi, all
I would like an advice. Can anyone suggest a better type of dishes to
use with glutheraldehyde and/or osmium tetroxide during fixation
time. Which bottles are better to store these fixatives?
Many thanks.

Patricia
____________________________
Patrícia João Reis (PhD student)
Escola Superior Biotecnologia
Universidade Católica Portuguesa
Rua Dr. António Bernardino Almeida
4200-072 Porto
PORTUGAL
Tel.: 225580044
Fax.: 225090351
e.mail: {mailto:pmreis-at-mail.esb.ucp.pt} pmreis-at-mail.esb.ucp.pt


From daemon Wed Sep 5 15:33:09 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Wed, 5 Sep 2001 16:24:05 -0400
Subject: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
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"Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
ETOH dehydration, CPD, mounting and SEM viewing.


O.k., we give up, searched numberous books, papers, catalogs, web
sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
"Champy's Solution"? Please?

Thank you much!

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Wed Sep 5 15:52:17 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Wed, 5 Sep 2001 16:50:49 -0400
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Are you referring to the fingers?


} We had some that would get caught, and the only thing to do with them
} after they were freed was to toss them. Don't try to straighten them.
}

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Wed Sep 5 17:38:32 2001



From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Wed, 05 Sep 2001 18:38:24 -0400
Subject: TEM of x-ray film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there knows how to run up an X-ray film (exposed as well as
unexposed) for TEM?????
Neelima Shah..............
Biomedical Imaging Core Facility
Uni of Pennsylvania
Philadelphia, Pa.




http://www.MED.upenn.edu/morphlab/



From daemon Wed Sep 5 18:36:19 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 05 Sep 2001 16:30:49 -0700
Subject: Re: LOMO water-immersion/dipping lenses for 160 mm-corrected

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LOMO (Leningrad's Opto-Mechanics Consortium) is well known manufacturer of
the fine optics in the Russia. Their microscopes are cheap (relatively)
and very suitable for students etc. LOMO also known as an manufacturer of
the biggest single-piece 6 meters diameter glass mirror for the telescope
situated in Caucasus mountains. That mirror was a biggest one in the world
at the time of inventing. I believe, they did also first full-quartz-optic
microscope for UV investigations and their MB-1/2 fluorescence microscopes
with "opak-illumination" were great.

I don't have any financial interest in this company.

Sergey Ryazantsev

At 10:03 AM 9/5/01 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 5 19:20:18 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Wed, 5 Sep 2001 18:13:55 -0600 (MDT)
Subject: Re: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ah, the wonders of a Google search! I found a wonderful histo manual at

http://131.229.114.77/histo/Manual.pdf

The recipe is given on p. 17 of this pdf but it is in code - the author
lists standard histology stock solutions and then gives the recipes for a
variety of fixatives by coding them from the stock solutions. Champy's is:
"35G; 20K; 12M; 24Q(9)" which means: 35 ml of 1% chromic acid (aq.), 20 ml
of 2% OsO4 (aq.), 12 ml of sat. potassium dichromate (9 g/ 100ml, aq.), 24
ml of distilled water, make up just before use. Sounds like nasty stuff.

I'm glad you asked - I'd have never found this pdf otherwise! (It is 113
pages and I haven't been able to figure out from where it originates -
some histology course). If anyone knows who wrote it, I'd like to know!

Tamara

On Wed, 5 Sep 2001, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Wed Sep 5 20:27:58 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Sep 2001 21:19:57 -0500
Subject: Sticky dots and Tacky Dots

Contents Retrieved from Microscopy Listserver Archives
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I think I saw some at "Fredericks" out here on the West Coast.
Contact me offline for the number.

Earl

----- Original Message -----
} From: {"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 05, 2001 12:42 PM


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Scott Walck wrote:
===================================================
I was at a talk where the carbon sticky dots were first presented. I wish I
could remember who was speaking. I know that SPI has the exclusive
distribution rights to them. I believe that they were developed by a group
at Dupont. I would check the literature and see if they published. Try
back issues of the meeting abstracts. I think that the talk that I went to
was about 10 years ago.
===================================================
Let me shed some light on this: SPI Supplies was (and is) the exclusive
licensee for the manufacturing and distribution of Tacky Dot™ Slides. The
patent holder was DuPont and the inventor was Dr. Alan Cairncross, still at
DuPont. I am guessing, but the paper you heard presented (at an MSA meeting
) which could have been about ten years ago (how time flies), probably was
presented by Dr. Cairncross (and other authors all at DuPont). Full
technical details are disclosed in US Patent # 5,356,751.

These should not be confused however with what are often times called by
others as "carbon sticky dots" or "double sided conductive adhesive tabs".
These are offered in various forms by SPI as well as the other main
worldwide distributors of consumable supplies for SEM applications.

The original poster (Wharton Sinkler) asked about adhesive "tabs" but did
not specify size. The largest dot size of the Tacky Dot Slides manufactured
by SPI Supplies is 300 µm in an orthogonal array on 2000 µm centers. I
think that Wharton was in essence asking about the smallest "carbon tab" or
"disc" that is available but the smallest that I know of are are 9 mm and
there are technical reasons why one could not go too much smaller than that
(in our opinion).

Chuck


===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Wed Sep 5 21:26:22 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Thu, 06 Sep 2001 12:25:13 +1000
Subject: Re:Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
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Champy:

1% chromic acid 7 ml
3% K2Cr2O7 7 ml
2% OsO4 4 ml

from "Notes on microscopical technique for zoologists" CFA Pantin Cambridge
Univ. Press 1962


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Wed Sep 5 23:26:45 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, September 05, 2001 7:26 PM
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The business of un-jamming JEOL plate camera doesn't have to be that gory.
Just remove a fuse located a couple inches left of the camera door (make
sure that little red light next to the fuse turns on), before reaching
inside, and no fingers will be shed.

Assuming that the camera mechanism is not damaged- simplest thing to do to
avoid jams- do not load film boxes to a full capacity(say, load 5 or 10
holders
less), make sure that film holders are in perfect shape - ditto on Sara's
comment, toss the damaged holders- and that the film holders are loaded
correctly. Also, little Teflon
rollers on the sides of the plate boxes must rotate freely (at a slight
touch, no friction) and must have spotless surface (no defects, scratches,
etc.). Replacement rollers are available from JEOL.
If you are going to unscrew 6 screws underneath the camera compartment in
order to pull the mechanism out (as was suggested in one of the previous
messages)- beware that those screws are separating camera vacuum from the
atmosphere. Make sure that the o-rings, screws, and seats are clean before
replacing the screws, to avoid vacuum leak.

If the mechanism is damaged- repair options will depend on you mechanical
aptitude. You are welcome to contact me off-line for further advice, yet I
agree that your best bet is to contact JEOL service representative.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Marie E. Cantino {cantino-at-uconnvm.uconn.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}





From daemon Thu Sep 6 02:34:50 2001



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 6 Sep 2001 00:24:47 -0700 (PDT)
Subject: Re: Bio. Fixation: What is Champy's Fluid?

Contents Retrieved from Microscopy Listserver Archives
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Champy's (1913) fixative formula is as follows:

sat. aqu. sol. phenol 200, 40% formaldehyde 48, trichloroacetic acid 3.6

Reference: 'The Microtomist's Formulary and Guide' by Peter Gray.
Blakiston Company, Inc., New York/Toronto. 1954:192 (794 pp.)

Cheers!
Ken

--------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

On Wed, 5 Sep 2001, Richard Edelmann wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}





From daemon Thu Sep 6 06:30:09 2001



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 6 Sep 2001 12:21:47 +0000
Subject: Scanning Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know what has happened to the journal Scanning Microscopy?

The latest part we have is Volume 10 pt.4 1998 and
our supplier cannot trace the publisher etc. all mail being marked
return to sender. Has the journal ceased publishing?

Chris Jeffree

==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Thu Sep 6 07:49:33 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Thu, 6 Sep 2001 07:37:49 -0500
Subject: RE: Sticky dots and Tacky Dots

Contents Retrieved from Microscopy Listserver Archives
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Actually, the size of the tabs is not the issue. Sorry about the confusion.

What I was asking about are indeed double-stick carbon tabs about 1 cm
diameter. I'd like to evenly space 48 of them on an aluminum plate, and am
looking for a less tedious way than by hand.

An alternative would be to simply cover the entire plate with a double-stick
conductive carbon layer, then depsoit the samples in the proper locations.
This may be cheaper than getting something custom made. Does anyone know
who might offer unperforated double stick conductive carbon material in
large dimensions (about 3 x 4 inches)?

Thanks,
Wharton

} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, September 05, 2001 9:20 PM
} To: MICROSCOPY BB
} Subject: Sticky dots and Tacky Dots
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Scott Walck wrote:
} ===================================================
} I was at a talk where the carbon sticky dots were first presented. I wish
} I
} could remember who was speaking. I know that SPI has the exclusive
} distribution rights to them. I believe that they were developed by a
} group
} at Dupont. I would check the literature and see if they published. Try
} back issues of the meeting abstracts. I think that the talk that I went
} to
} was about 10 years ago.
} ===================================================
} Let me shed some light on this: SPI Supplies was (and is) the exclusive
} licensee for the manufacturing and distribution of Tacky Dot(tm) Slides.
} The
} patent holder was DuPont and the inventor was Dr. Alan Cairncross, still
} at
} DuPont. I am guessing, but the paper you heard presented (at an MSA
} meeting
} ) which could have been about ten years ago (how time flies), probably was
} presented by Dr. Cairncross (and other authors all at DuPont). Full
} technical details are disclosed in US Patent # 5,356,751.
}
} These should not be confused however with what are often times called by
} others as "carbon sticky dots" or "double sided conductive adhesive tabs".
}
} These are offered in various forms by SPI as well as the other main
} worldwide distributors of consumable supplies for SEM applications.
}
} The original poster (Wharton Sinkler) asked about adhesive "tabs" but did
} not specify size. The largest dot size of the Tacky Dot Slides
} manufactured
} by SPI Supplies is 300 µm in an orthogonal array on 2000 µm centers. I
} think that Wharton was in essence asking about the smallest "carbon tab"
} or
} "disc" that is available but the smallest that I know of are are 9 mm and
} there are technical reasons why one could not go too much smaller than
} that
} (in our opinion).
}
} Chuck
}
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}


From daemon Thu Sep 6 08:31:40 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 6 Sep 2001 09:24:09 -0400
Subject: Re: Bio. Fixation: What is Champy's Fluid? Found.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for all who replied we now have a recipe to try out.

Champy's:

=========

1% chromic acid 7 ml
3% K2Cr2O7 7 ml
2% OsO4 4 ml

from "Notes on microscopical technique for zoologists" CFA Pantin
Cambridge
Univ. Press 1962

========

"[3% aqueous potassium dichromate; 1% aqueous chromic acid; 2%
aqueous solution of osmic acid (osmium tetroxide); (2:2:1)]" Page 804-805
Samuel Thompson's "Selected Histochemical and Histopathological
Methods", 1966.

==========

"35G; 20K; 12M; 24Q(9)" which means: 35 ml of 1% chromic acid (aq.), 20
ml of 2% OsO4 (aq.), 12 ml of sat. potassium dichromate (9 g/ 100ml, aq.),
24ml of distilled water, make up just before use. Sounds like nasty stuff.

===========



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Thu Sep 6 09:25:41 2001



From: Jeff Hedlesky :      jhedlesky-at-edgebb.net
Date: Thu, 06 Sep 2001 09:18:06 -0500
Subject: Feigl's Solution?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone out there know: a) where to buy this stuff? b) How to
make this stuff? or c) where I can find any articles, mentions or
instructions on the use of this stuff? (other than the Fisheries
Science article that comes up in Google) I've been told that it's a
useful marker for the aragonite polymorph of CaCO3. (Thanks, John Hunt
at CCMR!) Any feedback, however oblique, would be appreciated.

Jeff

--
Jeff Hedlesky
Aladdin Companies
151 N. Main, Suite 700
Wichita, KS 67202

316.265.8913 voice
316.265.7014 FAX
jhedlesky-at-edgebb.net



From daemon Thu Sep 6 09:25:44 2001



From: Bradley Starcevich :      microscopist-at-excite.com
Date: Thu, 6 Sep 2001 07:17:39 -0700 (PDT)
Subject: Lomo optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My experience with LOMO microscopes and optics
has been positive as well. Their products are
an incredible value, the quality is very good.

I'd buy another LOMO product in a heartbeat.

I do not represent LOMO. I have no financial
interest in the company.

Bradley K. Starcevich
Director, Research and Development
The Cougar Group
1215 Atlantic Drive
West Chicago, IL USA 60185





_______________________________________________________
http://inbox.excite.com




From daemon Thu Sep 6 11:29:05 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Thu, 6 Sep 2001 11:18:09 -0500
Subject: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to the list.

It's been a while since I've been on the list, and since I've been working
as a microscopist.

If I image a sample in the SEM with the sample tilted, then want to make
measurements from the images, what is the equation to use to correct for the
tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
drawing a blank.

Thanks for your assistance.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Sep 6 12:30:24 2001



From: Axel Blau :      axel-at-caltech.edu
Date: Thu, 6 Sep 2001 10:22:08 -0700
Subject: Re: LOMO water-immersion/dipping lenses for 160 mm-correctedscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to the two of you for the information. The website -at-
www.comet.net/gek/ is very interesting, and I did not know that there was
some other distributor in the US. I have to agree that the quality of the
lenses we got is outstanding for the price we paid.

With best regards,

Axel

----- Original Message -----
} From: Jeff Hedlesky
To: Sergey Ryazantsev
Cc: Axel Blau ; Microscopy-at-sparc5.microscopy.com
Sent: Thursday, September 06, 2001 6:56 AM





From daemon Thu Sep 6 12:34:07 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 6 Sep 2001 13:28:48 -0400
Subject: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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For a flat sample with tilted to an angle, theta, the image perpendicular to the tilt axis is foreshortened by cos(theta). Take your measurement on the tilted image and divide by cos(theta).

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
Sent: Thursday, September 06, 2001 12:18 PM
To: 'microscopy-at-msa.microscopy.com'


Hello to the list.

It's been a while since I've been on the list, and since I've been working
as a microscopist.

If I image a sample in the SEM with the sample tilted, then want to make
measurements from the images, what is the equation to use to correct for the
tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
drawing a blank.

Thanks for your assistance.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Thu Sep 6 12:49:29 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 06 Sep 2001 12:42:18 -0500
Subject: Re: SEM: Tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After tilting, the feature you want to measure will form the hypotenuse of
a triangle. The horizontal projection you see will be the side of the
triangle adjacent to the tilt angle. One side of the feature will be
elevated with respect to the other and that amount will be the length of
the side opposite the tilt angle.

The cosine of an angle is the ratio of the length of the adjacent side to
the length of the hypotenuse. Therefore, you would use the following formula.

hypotenuse = adjacent/cosine, or

real length - observed length/cosine(tilt angle).

At 11:18 AM 9/6/2001 -0500, you wrote:

} Hello to the list.
}
} It's been a while since I've been on the list, and since I've been working
} as a microscopist.
}
} If I image a sample in the SEM with the sample tilted, then want to make
} measurements from the images, what is the equation to use to correct for the
} tilt? I know it involves the cosine of the tilt angle, but beyond that, I'm
} drawing a blank.
}
} Thanks for your assistance.
}
} Nancy Zjaba, Technical Staff
} Alfalight Inc.
} 1832 Wright Street
} Madison, WI 53704
} (608) 240-4875
} nzjaba-at-alfalight.com



From daemon Thu Sep 6 13:28:30 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 06 Sep 2001 11:21:58 -0700
Subject: Re: removing jammed TEM film cassettes

Contents Retrieved from Microscopy Listserver Archives
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I was working with JEOL microscopes (100B, 100C, 100CX, 1200EX) for a few
decades. Over all this time I do remember just a few accidents related to
the camera mechanics in the microscopes. In all that cases the "human
factor" was involved and usually the problem was improperly loaded into
cassette the piece of the film. If film is not in place, sometime it cover
one of the square slots on the cassette and "fork" could not catch the
cassette properly. In this case the forks usually stick in the gap between
two set of rails in the camera mechanism. As it was mentioned in the most
replies, the solution is to move up those forks out of the gap. Usually I
am doing so using flexible metal ruler with fuse OFF (great comment
Vitaly). The bad things about JEOL camera design that it has extremely
powerful motor with huge gear which is able to bent and ruin all rails in
the camera if working improperly. Magically, it was never happens with my
microscopes, but when I look inside the camera and see that huge gears I am
worrying anyway.

Sergey

At 12:12 AM 9/6/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 6 14:35:40 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Sep 2001 15:26:37 -0500
Subject: Large Double Sided Adhesive Conductive Carbon Sheets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Wharton Sinkler wrote:
=================================================
Actually, the size of the tabs is not the issue. Sorry about the confusion.

What I was asking about are indeed double-stick carbon tabs about 1 cm
diameter. I'd like to evenly space 48 of them on an aluminum plate, and am
looking for a less tedious way than by hand.

An alternative would be to simply cover the entire plate with a double-stick
conductive carbon layer, then depsoit the samples in the proper locations.
This may be cheaper than getting something custom made. Does anyone know
who might offer unperforated double stick conductive carbon material in
large dimensions (about 3 x 4 inches)?
==================================================
We at SPI Supplies offer as a standard off-the-shelf product a sheet that is
500mm x 500 mm. You could cut out from that standard size sheet whatever
size you might require. This is described on URL
http://www.2spi.com/catalog/spec_prep/cond_adhes-sheets.html

This is the "master material" from which the double sided conductive carbon
adhesive discs are fabricated.

We have heard of another customer who has made a template in the orthogonal
arrangement required and then by hand is affixing (on large sheets) what
they want to attach through the use of the template, which is suspended
slightly above the adhesive and never actually contacts the adhesive. That
would be the cheapest of the various alternatives.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Sep 6 14:53:19 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 15:46:24 -0400
Subject: Re: About working with fixatives

Contents Retrieved from Microscopy Listserver Archives
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I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
with a Teflon lined screw cap. The bottle is then held in the refrigerator
in a small plastic jar (available from all lab distributors - mine from
Fisher or S/P) with a screw cap . In this way, the plastic becomes
osmicated NOT the refrigerator. I have done this for years with no untoward
results.

I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
plastic. Osmication is done in glass vials with Teflon lined screw caps
using a minimum of OsO4 to do the job.

I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
Teflon lined cap. I then make up an appropriate amount of fixative using
the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
in a plastic jar in the refrigerator.

I find that double containers are safe and reduce contamination
considerably.

Hope this helps.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Thu Sep 6 15:45:37 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Thu, 06 Sep 2001 13:37:41 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Sep 6 16:25:34 2001



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Thu, 6 Sep 2001 16:18:19 -0500
Subject: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
I have a researcher here that is having some difficulty with getting good
histology at the EM level. She is fixing Drosophlia brains with 4%para/0.5%
glut in phosphate buffer pH7.2, no osmium post fix, ethanol dehaydration,
propylene oxide as a transitional solvent to polybed 812, then performing post
sectioning immunolabeling. The tissue has "holes" in it when viewed at the
TEM. They are not holes in the section, but appear to be voids in the tissue.
We have tried leaving out the propylene oxide, we have osmicated, and have
looked at osmotic strength of the fixative (and many variations of the above,
to no avail). Can anyone offer advice?
Randy Nessler
Univ. of Iowa



From daemon Thu Sep 6 16:25:51 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 6 Sep 2001 16:19:20 -0500
Subject: RE: Tilt correction factor

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R = T/(M*cosA)

R - "real measurements"
T - measurements on a tilted specimen
A - angle
M - magnification

But you can use it only if your specimens is flat.
Otherwise you should use stereo measurements.

Good luck,

Vladimir



Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Nancy Zjaba [mailto:nzjaba-at-alfalight.com]
} Sent: Thursday, September 06, 2001 11:18 AM
} To: 'microscopy-at-msa.microscopy.com'
} Subject: SEM: Tilt correction factor
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hello to the list.
}
} It's been a while since I've been on the list, and since I've
} been working
} as a microscopist.
}
} If I image a sample in the SEM with the sample tilted, then
} want to make
} measurements from the images, what is the equation to use to
} correct for the
} tilt? I know it involves the cosine of the tilt angle, but
} beyond that, I'm
} drawing a blank.
}
} Thanks for your assistance.
}
} Nancy Zjaba, Technical Staff
} Alfalight Inc.
} 1832 Wright Street
} Madison, WI 53704
} (608) 240-4875
} nzjaba-at-alfalight.com
}
}
}
}


From daemon Thu Sep 6 16:55:01 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 06 Sep 2001 16:47:21 -0500
Subject: Ethanol vs. Acetone for critical point drying

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Hi,

Recently, a collegue was having problems with a white crystaline
substance covering his samples of ear tissue after they were removed
from the critical point dryer. It was believed to be from a
contaminant in the ethanol and it was recommended to switch to acetone
dehydration as it was less likely to have this problem. I haven't done
SEM in years, so I haven't been paying attention to anything like this
on this listserve. I was wondering if other people experiencing this
problem. If you are using acetone, have you ever seen this?

Thanks for your comments,

Karen Pawlowski, Ph.D.


From daemon Thu Sep 6 18:03:12 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 6 Sep 2001 18:26:24 -0500
Subject: Re: Ethanol vs. Acetone for critical point drying

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Depends upon the geometry of the sample: try making the calculations for a
sphere at a 45 degree angle.

My guess is that the measurements would be the same as if the sphere were at
0 degrees or 90 degrees.

For purely planar sample the equations would be valid.

regards,

Earl

----- Original Message -----
} From: "Nancy Zjaba" {nzjaba-at-alfalight.com}
To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 06, 2001 9:18 AM


Karen,

One possible source for this contaminant is from the use of drying
agents such as molecular sieves or other crystals. Unless one is
extremely careful, the fine crystals get suspended in the liquid
phase and dry down onto the specimen. We no longer use drying agents
in our ethanol but use absolute ethanol directly from small (400-500
ml) bottles. If the bottles have been opened for awhile, then we use
the alcohol to prepare a dehydration series since it is no longer
anhydrous.

You CAN still use molecular sieves, etc. but we prepare them one
month before use and take care not to jostle the bottles to any
extent.

Another source for the crystalline material may be from prior
contamination of the chamber by someone who had a dirty sample or
contaminated ethanol.

JB



} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Sep 6 18:35:58 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 06 Sep 2001 21:57:47 -0400
Subject: Un-jammed and thanks

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The address for your LOMO dealer is:
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
fax 732 758 9142
----- Original Message -----
} From: "Bradley Starcevich" {microscopist-at-excite.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 06, 2001 10:17 AM


Once again, my plea for help to you friendly and knowlegeable folks on
this listserve produced twelve hints, warnings to protect my fingers and
encouragement to keep smiling through equipment hassles. I have compiled
them into a lengthy word document and would be happy to send it to anyone
requesting it. The following were the most important bits of advice I
received from all of you and from a discussion with one of JEOL's excellent
service techs working out of Pittsburgh
a) the camera mechanics are pretty much the same (100, 1200, 2010 even)
b) if an error message indicating the "no film loaded" appears on the
computer screen, remove the fuse which turns the mechanism's motor off
(preventing the motor from burning out and your fingers should you elect
to remove film + plate)
c) examine plates--this one was "bowed" and showed a wear pattern
d) don't load the film box to capacity
e) dispose of damaged plates, even those with minor bends
f) examine the teflon rollers on the film boxes, replace if they do not
rotate freely

Thanks again to: Sergey Ryazantsev, UCLA; Robin Cross, Rhodes University,
South Africa; Keith Moulding, Hong Kong University of Science and
Technology, Hong Kong; Deb Stenzel Queensland Univ. of Tech, Brisbane,
Australia: . John Hunt, Cornell: John Humenansky/Staff Scientist Physical
Electronics, Inc. (PHI); Malcolm Haswell, UK; Richard Beanland, Structural
Analysis Lab,Caswell Technology; Sara Miller, Duke University; Wilbur C.
Bigelow, University of Michigan; Marie E. Cantino, University of
Connecticut; Vitaly Feingold Scientific Instruments and Applications.

Rosemary Walsh, PSU







From daemon Fri Sep 7 04:43:07 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 7 Sep 2001 10:30:55 +0100 (GMT Daylight Time)
Subject: Question about preparing osmium tetroxide

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While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 7 04:43:11 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Fri, 7 Sep 2001 19:35:43 +1000
Subject: RE: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Using clean ethanol (does not need to be "dried" for CPD) would be a lot easier
than replacing seals - if the valves in your CPD don't like acetone.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, September 07, 2001 7:47 AM, Karen Pawlowski
[SMTP:kpawlow-at-swbell.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.


From daemon Fri Sep 7 05:35:35 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 16:53:08 -0400
Subject: RE: Camera Lucida drawing tube (Zeiss) questions

Contents Retrieved from Microscopy Listserver Archives
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I would first like to thank the list members for the responses I have
received. Thanks are also due to software support at Synoptics for a
prompt and informative reply to my e-mail. I provide here a brief
description of how the problem was resolved finally, in the hope that
the info may be useful to somebody someday, just as I have learned a
lot from this list.

On checking the binary file from the SGI computer in a hex viewer, I
confirmed that the bytes were not in the format required by Semper for
a Fortran unformatted file. Also confirmed that this was not due to
file transfer by explicitly setting the transfer mode in ftp to binary.
The e-mail from Synoptics informed that the Fortran unformatted format
was not intended to be portable across operating systems and different
hardware and that the input / output commands in Semper supersede the
read / write commands. I discovered that the format is not even
portable from the DOS version to the Windows version of Semper. The
main suggested solution was to convert the image to one of the standard
binary image formats such as tiff that can be read into Semper.

Two solutions are possible:
(i) the tv1 program in EMS can be used to display the EMS image and the
screen can be exported to Semper format. This export file has the
extension eix and is an ASCII file with the image pixel data as
integers. When read into Semper it is available as a byte class image.
Problem with this approach is that the pixel data floating point values
are converted to 8-bit values and I am not sure if this affects further
quantification. Also, it requires selecting the area to be saved and I
tend to end up with odd sized images which included bits of the screen
background requiring further cropping with Semper.

(ii) To change the source code of se1 such that all unformatted Fortran
write statements are replaced with formatted write with the format
strings as per Semper file format specifications. On compiling and
running, this results in an ASCII file which can be read into Semper as
a floating point image, that is, without any change in the pixel
values. This is the solution I have adopted. The resulting file size is
larger by about 3.5 times but this is not a limitation for me. I am
still left with the minor problem that the title of image is getting
written as a string of blank characters. My Fortran is a bit rusty and
I hope to solve this soon.

Best Wishes to all.

-----Original Message-----
} From: Divakar [SMTP:divakar-at-igcar.ernet.in]
Sent: Tuesday, September 04, 2001 10:33 AM
To: 'Microscopy-at-sparc5.microscopy.com'


Hi Alan,
Go get em! I have a Camera Lucida for a Leitz Ortholux that I have
sitting next to me in my office.
The collar that I have fits over the narrow diameter eyepiece tube
on my scope and sets my Camera Lucida at the exit pupil of the ocular. This
permits very close concordance of the two images transmitted by the prism.
Of course, my camera Lucida is an old thing with a 4x6" mirror hanging off
to the right. My drawing surface must be inclined, because my oculars are
inclined on my binocular headed microscope. I had to construct the
inclined table, but it works anyway.
Here's a project. Take a piece of paper and mark it off in half
centimeters (i.e., every 5mm, Ho! Ho!). Using a high mag objective look at
something small like a diatom. Using the graduations on the fine focus knob
change focus by one graduation and move the paper one 5mm increment drawing
the outline of the in-focus diatom at each point. You will quickly see how
to limit your lines so they don't overlap. Try to predict what the result
will be after you draw several outlines.
The key to getting everything right is to balance the illumination
of the paper with that of the scope, but you probably noticed that already.
Send me a photo by direct eMail. The listserver doesn't convey
attachments.

You are welcome to ask questions any time or to discuss,
What kind of scope(S) do you have? Illuminators? Use high intensity on the
paper!


Regards,

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail (Work): fmonson-at-wcupa.edu
eMail (Home): monson_fc-at-msn.com
Please call before visiting



} ----------
} From: Alan Davis
} Sent: Wednesday, September 5, 2001 7:16 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Camera Lucida drawing tube (Zeiss) questions
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have gotten ahold of a Zeiss camera lucida drawing tube. It has a
} mirror at an angle that allows one to draw underneath the inclined eye
} tube. Works for a Zeiss Standard 16. I am getting the hang of it a bit.
} However, I have at least one question:
}
} What is the collar for (referred to as "ring clamp" below)? It is
} somewhat eccentric, but I cannot imagine any purpose for it. The device
} doesn't fit over it, but nearly does. It *is* useful for attaching it to
} a wider eyepiece tube.
}
} This plastic collar fits snugly inside the eyepiece tube of an Olympus
} SZH; this is good. Also, it fits over the end of the standard 16 eye
} tube, with a barrier that doesn't allow it to slip down over the tube. i
} have some documentation for a similar drawing tube, but there is a slight
} difference in the picture (there is a knurled knob at the point where the
} mirror/prism fits over the eyepiece. Mine doesn't have that, it fits
} snug). Here is the relevant section from the documentation for the
} pictured device:
}
} 1. Focus microscope.
}
} 2. Remove eyepiece and slip ring clamp on to tube. Replace eyepiece
} and fix with ring clamp. [I referred to the "ring clamp" as a
} collar.]
}
} 3. Clamp drawing prism on the eyepiece. [I assume this refers to the
} knurled
} screw that isn't present on my device---mine doesn't need to be
} clamped on.]
}
}
}
} } From reading the description, though, I don't know what it is for. Is it
} a spacer to move the eyepiece up higher? Which Zeiss is it really
} designed for?
}
} Also, I would greatly appreciate any tips. The tips I found in MICSCAPE
} were helpful. Can I initiate some correspondence with someone who has
} some experience with one of these fantastic devices?
}
} Alan Davis
} Marianas High School
}
} --
} adavis-at-saipan.com 1-670-235-6580
} Alan E. Davis, PMB 30, Box 10006, Saipan, MP 96950-8906, CNMI
}
} I have steadily endeavored to keep my mind free, so as to give up any
} hypothesis, however much beloved -- and I cannot resist forming one
} on every subject -- as soon as facts are shown to be opposed to it.
} -- Charles Darwin (1809-1882)
}
}
}
}
}
}


From daemon Fri Sep 7 06:20:45 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 6 Sep 2001 17:57:48 -0400
Subject: Re: Plate Jam is Tasty

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According to Prof Bigelow, one clears such a jam with a wiggle! But
from the Eastern Hemisphere and Dr. Moulding we hear that such jams are
freed by a waggle. Now what needs to be established is whether one should
wiggle or waggle, or whether one must learn to do both, AND whether TEM's
behave differently toward wiggling and waggling in different places (We all
know JEOL is 100V no matter where you find it.).
Having been raised in North America, I have been exposed to what has
always been described as typical wiggling, and I would hope that if I ever
saw someone waggle, I would know that waggling was being done. One might be
terribly embarrassed if one were to characterize a wiggle as a waggle. For
example, is there wiggling or waggling in typical down-under dancing? If
one stands in Hong Kong ogling the girls as they pass by, is one traumatized
by the wiggle or the waggle?
We know that one can wiggle a nose, but conversely one can only wag
one's tail - in North America. But then, we all have seen buns wiggle.
Perhaps
the relationship is something like this. A waggle could be a small wag, and
since we know that Prof. Bigelow was not suggesting that one should wag the
plate
to free it, rather he suggested that we just give it a little wiggle. In
this way one could assert no difference between a wiggle and a waggle, both
being, similarly, small wags. One would not say, "Wag the plate a little."
But one could "Waggle it!" or just "wag it a little!" This would all be so
much more clear if dogs in
North America were known to "wig their tails" when they are happy. Perhaps
what we have is an hemispheric etymological inversion such that in the
West, wiggle is to wag, where as, in the East, waggle is to wig!
We here in the Western Hemisphere have learned to watch worms
wiggle, but what do they do elsewhere, I wonder? Might a waggle merely be
out of phase with a wiggle? Sine! Cosine! Do these differences occur,
because of the differences between 50 and 60 Hz? Perhaps there might be NSF
or WHO money for such a question.

Oh well, ruminating like a mad sow, he demurred, "No rumen in the old sow at
all."

If anyone can clear any of this up, I certainly would like to know!

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Fri Sep 7 07:30:36 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 08:21:24 -0400
Subject: RE: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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Always an interesting question.

While I have usually come to a comfortable resolution with respect
to this kind of safety issue, this one has proved elusive. So.....

My normal procedure is to unwrap and clean the ampoule as I would my
best glassware. That is, I treat it to a "biochemistry lab" cleaning,
finishing with 3X rinses in sgdd(single glass-distilled deionized) HOH.
Between the last two waters AND WEARING FRESH POWDER-FREE GLOVES, I score
the ampoule three times with an ampoule file while holding it on a folded
paper towel in the bottom of a shallow heavy-glass bowl. I then transfer
the ampoule to an appropriate 100ml bottle (I use a serum bottle, because it
is constructed of thick glass, shows imperfections readily (especially
around the bottom), and can withstand the breaking of the ampoule). The
scored ampoule breaks more readily than an unscored one, and three scores
(~1cm apart) provide sufficient weakness for breakage to occur with relative
ease. To further facilitate breakage, I add only some of the total amount
of solvent HOH (for me, 25ml) sufficient to keep the ampoule from floating
while providing enough for initial immersion of the crystals. Finally, I
rest the serum bottle on the same folded paper towel in the same thick glass
bowl to dissipate the shock of the breaking regimen. NOTE: Since it is at
this part of the process at which failure has occurred (twice in 25 years
the bottom of the bottle has separated from the barrel), I am still working
in the hood, but with a substantial amount of 95% EtOH at hand to render the
OsO4 harmless if I have an accident.

If the above smacks of science, please forgive the allusion. The
method and approach are the result of what has evolved over time through
trial and error and the application of common sense to a continuing, natty
problem.

I have always hoped that someone would supply a FINAL solution so we
can all be more comfortable when "Stock solution" time arrives.

Regards,

Fred Monson




} ----------
} From: Patton, David
} Sent: Friday, September 7, 2001 5:30 AM
} To: Monson, Frederick C.
} Cc: 'Microscopy Listserver'
} Subject: Question about preparing osmium tetroxide
}
} While we are on the topic of making up fixatives I have a
} supplementary question.
}
} How do you open glass osmium tetroxide ampoules?
}
} I put mine in a 100ml glass bottle and then break it with a
} glass rod. This is nerve-wracking, tedious (they do not
} break readily) and not as safe a procedure as I would like.
}
} The ampoules used to have a weakened neck which could be
} sawn off but they do not come in this form from our
} supplier any more.
}
} Is there an easier way?
}
} Dave
}
}
} On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
} {fmonson-at-wcupa.edu} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I dissolve 1g OsO4 in 25ml double distilled water and store it in a
} bottle
} } with a Teflon lined screw cap. The bottle is then held in the
} refrigerator
} } in a small plastic jar (available from all lab distributors - mine from
} } Fisher or S/P) with a screw cap . In this way, the plastic becomes
} } osmicated NOT the refrigerator. I have done this for years with no
} untoward
} } results.
} }
} } I fix with aldehydes in screw cap scintillation vials (20ml or 5ml)
} glass or
} } plastic. Osmication is done in glass vials with Teflon lined screw caps
} } using a minimum of OsO4 to do the job.
} }
} } I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml
} dd
} } HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with
} a
} } Teflon lined cap. I then make up an appropriate amount of fixative
} using
} } the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
} kept
} } in a plastic jar in the refrigerator.
} }
} } I find that double containers are safe and reduce contamination
} } considerably.
} }
} } Hope this helps.
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging(CASI)
} } West Chester University of Pennsylvania
} } Schmucker Science Center II
} } South Church Street
} } West Chester, PA, 19383
} } eMail: fmonson-at-wcupa.edu
} } http://darwin.wcupa.edu/casi/
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}


From daemon Fri Sep 7 08:02:18 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 7 Sep 2001 14:48:48 +0200
Subject: Re: Tilt correction factor

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Depends also from the measurement direction. The formula is right in the
tilt direction, but the real tilt angle of a straight line on flat sample
varies from nominal tilt angle in tilt direction to zero in the
perpendicular direction. In the other directions, the formula is right
too, but you must estimate the real tilt angle in the measurement
direction, in respect to the tilt direction.

And of course, don't forget than in a SEM the perspective is not "conique"
(I don't know the right terms in english) but "cavalière" . A tilted
square looks like a rectangle and not a trapezoid. Try with a TEM grid
with square holes, and try too with a grid with hexagonal holes. And
rotate it ... You may feel dizzy, with high tilt angles. Our brain is not
build for such a perspective ! But it can give nice pictures.



J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 6 Sep 2001, Earl Weltmer wrote:

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}
}
} Depends upon the geometry of the sample: try making the calculations for a
} sphere at a 45 degree angle.
}
} My guess is that the measurements would be the same as if the sphere were at
} 0 degrees or 90 degrees.
}
} For purely planar sample the equations would be valid.
}
} regards,
}
} Earl
}
} ----- Original Message -----
} } From: "Nancy Zjaba" {nzjaba-at-alfalight.com}
} To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
} Sent: Thursday, September 06, 2001 9:18 AM
} Subject: SEM: Tilt correction factor
}
}
} } ------------------------------------------------------------------------
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} } -----------------------------------------------------------------------.
} }
} }
} } Hello to the list.
} }
} } It's been a while since I've been on the list, and since I've been working
} } as a microscopist.
} }
} } If I image a sample in the SEM with the sample tilted, then want to make
} } measurements from the images, what is the equation to use to correct for
} the
} } tilt? I know it involves the cosine of the tilt angle, but beyond that,
} I'm
} } drawing a blank.
} }
} } Thanks for your assistance.
} }
} } Nancy Zjaba, Technical Staff
} } Alfalight Inc.
} } 1832 Wright Street
} } Madison, WI 53704
} } (608) 240-4875
} } nzjaba-at-alfalight.com
} }
} }
} }
}
}



From daemon Fri Sep 7 08:12:57 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 7 Sep 2001 09:11:37 -0400
Subject: Re: Question about preparing osmium tetroxide

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The method we use, which I teach to all the students, is to wrap the vial
with a short length of paper towel (usually about four or five turns around
the bottle), grasp the vial at both ends, and gently exert pressure with
the thumbs. Then carefully unwrap the package and throw both vial halves
in warm distilled water. I've never had any problem, either with crystals
falling out in the paper or with glass poking through the paper towels. I
was also taught the whack method, and found that my aim was not adequate to
the job.

We use the same wrapping method with glutaraldehyde ampoules, but instruct
the students to be sure to keep them upright.

Marie
}
} While we are on the topic of making up fixatives I have a
} supplementary question.
}
} How do you open glass osmium tetroxide ampoules?

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Fri Sep 7 08:17:08 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 7 Sep 2001 14:14:45 +0100 ()
Subject: Re: SEM: Tilt correction factor

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To Nancy Zjaba and all Listers,

Regarding tilt correction, I enclose this from an earlier reply: it is a
warning against the use of the tlit correction control on the SEM.

*** one thing is that [certain] morphology is generally quite low-profile
and vague under SEM, _unless_ you tilt the specimen: I generally tilt at
54^ = ARC COS (1 / SQRT(3)), the "magic" angle which ensures that x, y,
and z come out isometric, with the axes at 120^ to each other: if there
is any orientation (say in an injection moulding or drawn specimen, then
while the specimen is still flat-on, make sure that x and y are both at
45^ to the tilt axis. Then you will get isometric projection of all three
axes. But NEVER use the tilt-correction control, or you will get
distortion: for example, it makes spheres (which should appear circular at
any angle) come out as ellipsoids.


+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+







From daemon Fri Sep 7 08:32:58 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 7 Sep 2001 08:26:14 -0500
Subject: Re: Question about preparing osmium tetroxide

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I have a diamond pen or scribe (bought mine from EMS) that I normally
use for indelibly etching numbers on glass slides. I use it to
carefully scratch a circular line completely around the narrowmost
point of the ampoule. I usually go around several times to ensure a
good scratch. I rinse the vial off with 95% ethanol and place inside
a sturdy glass bottle (I use a Schott-brand glass bottle with orange
plastic cap - available from major supply houses like fischer). I
then shake the bottle vigorously (inside the fume hood, while
silently praying it doesn't break!) It generally breaks within a few
good snaps of the wrist. I add 50 mls of water and then store this
bottle inside a plastic jar in my fume hood. The inside top of the
plastic cap of the Schott bottle and the inside of the plastic jar go
black with time and I periodically (yearly) replace them. I would
never trust putting this stuff in my refrigerator. It leaks out the
first bottle - why would one think it doesn't then leak out the
second? I use it up usually within 3-4 months and never had a
problem with room temperature storage over the last 20 years. If you
use ampoules with pre-scored lines (easy snap open style) there is
another trick to consider i used to use in the old days but it comes
with a potential hazard that safety officers and lawyers advise
against so I won't recommend it. Place about one cm of liquid
nitrogen in a 50 ml disposable beaker in the hood. Slowly lower the
ampoule into the liquid nitrogen. this causes the osmium to contract
and break free from the glass. Snap the ampoule and pour the
contents into water. the low temp also reduces fumes during pouring.
this method avoids glass in your stock solution. the danger is that
if there is a crack in your vial, the nitrogen would enter, and when
warmed, potentially explode the vial. I never tried dry ice but i
suspect it would work and avoid this hazard.

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--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Sep 7 08:54:28 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 7 Sep 2001 08:52:19 -0500
Subject: Re: Ethanol vs. Acetone for critical point drying

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Karen,

I have done CPD for years, and never seen this problem with EtOH
dehydration (or acetone). The only times I've gotten anything like
this is from the specimen container used for drying, or salt
precipitation when the buffer was incompletely washed off. Another
possibility is the CO2. Many CO2 tanks are full of water and crud. Is
there a molecular sieve filter (for the water) and a membrane filter
(for the crud) on the line from the tank to the CPD?

Mind, it contaminant *could* be from the EtOH, but that's just a
reason to use better EtOH, not to switch to acetone.

Phil

} Hi,
}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Fri Sep 7 10:30:21 2001



From: Krueger, Eugene W. :      krueger.eugene-at-mayo.edu
Date: Fri, 7 Sep 2001 10:23:26 -0500
Subject: 3-d reconstructions from serial sections

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Hello all,
I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you choose.
Also vendors feel free to send me any info you might have on the products
you sell.

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology II
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/


From daemon Fri Sep 7 13:54:49 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 7 Sep 2001 14:45:06 -0400
Subject: Re: Ethanol vs. Acetone for critical point drying

Contents Retrieved from Microscopy Listserver Archives
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Karen:

Another source that we have come across is sodium phosphate buffer
crystals. If fixation is carried out with sodium phosphate buffered soluitons,
and then immediately transittioned to dehydration solvent (either EtOH or
Acetone) then we have seen samples coated with fine crystals. We use
either serveral water rinses between fixation and dehydration or an alternative
buffer (i.e. Na Cacodylate)

}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Fri Sep 7 14:13:11 2001



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 07 Sep 2001 12:05:01 -0700
Subject: Electron Microscopes: Electric Trains, Buses, Subway

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I would be interested in hearing from people anywhere in the world who have
had PERSONAL experiences with the installation of electron microscopes or
other sensitive equipment within 800 meters of electrically powered light
rail, trains, buses or subway systems which have created vibrations or
stray field problems for these instruments. I am specifically interested
in hearing from those who have had prior experiences with light rail or bus
transportation units powered by electricity. I would like to hear positive
(no known problems for sensitive instruments) as well as negative
experiences you have had or are having.

If you are aware that one or more of these types of transportation systems
is within the 800 meter range of your equipment and specific measures were
taken to eliminate vibration or stray fields, I will like to know that too.


You may wish to respond to me personally. Thank you.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Sep 7 14:55:48 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Friday, September 7, 2001 4:30 AM
Subject: Fwd: Question about preparing osmium tetroxide

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dave,
I personally would not use a supplier who does not pack Osmium in easy open vials or ampoules such as the ones which have the thin neck that can be snapped open. It is just too dangerous to try to break the vials as you described.

Often the osmium crystals will adhere tightly to the glass. If you dip the vial into liquid nitrogen while holding it by the neck with forceps, the crystals will release from the glass surface. They can then be shaken down to one end of the vial.

You can purchase silicon ampoule breakers from some of the major EM supply houses. They are flexible molded silicon with a depression the shape ofthe ampoule. Just put the ampoule in, bend the mold and the ampoule will break. Then remove the portion with the crystals and pore them into your osmium container. You end up with the two large piece of glass which should be disposed of carefully since there might be traces of osmium remaining. The osmium crystals will normally dissolve into water (or buffer) if left overnight at room temperature....in a hood.

I normally make osmium up double strength in water and it will keep for months in the refrigerator, remaining a clear light amber solution. I also recommend double bottling to help prevent escape of vapors. However, we do not store any biologicals in the same refrigerator with fixatives. Regardless of how careful you try to be, the odds are that some fixative vapors will escape.

Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907



--------------------------------------


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"





From daemon Fri Sep 7 16:02:46 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Fri, 7 Sep 2001 15:54:34 -0500
Subject: Thanks for tilt correction factor

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who responded to this question. The most often-cited equation
was:

Real length = Observed length/cosine (tilt angle)

Several people noted that sample geometry plays a role, and that this
equation is useful only for flat samples.

Have a good weekend, everyone.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com



From daemon Fri Sep 7 16:10:39 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 17:05:33 -0400
Subject: RE: Bio. Fixation: Which Champy's?

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Gray, Peter, 1975, Microtomist's Formulary and Guide, R. Krieger,
Huntington, NY. (Still available for the hardy bibliophile at
http://WWW.WEB4U.COM/cgi-bin/full_page?0-88275-247-2) reports FOUR fixative
mixtures that are attributed directly to Champy:

1. Sat. aq. phenol(200ml)+40%HCHO(48ml)+Trichloroacetic
acid(3.6g)

2. HOH(20)+2% CrO3(50)+pyrolignious acid (35)(URL:
http://www.ayrshirehistory.org.uk/AcidWorks/acidworks.htm#Pyroligneous%20Aci
d)

3. HOH(33)+2% OsO4(22)+2%CrO3(20)+7.5% K2Cr2O7(16)

4. HOH(60)+7.5% K2Cr2O7(40)

My favorite, of course, is number 2!!! whose last component provides a
wonderful bit of chemical history. We see how Bayer must have started.
Dupont.

Regards, and I know that this doesn't help at all!

But it is all part of the art.

Fred Monson









} ----------
} From: Richard Edelmann
} Reply To: edelmare-at-muohio.edu
} Sent: Wednesday, September 5, 2001 4:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Bio. Fixation: What is Champy's Fluid?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed
} by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}


From daemon Fri Sep 7 16:37:45 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 07 Sep 2001 17:35:51 -0400
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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EMS sells an ampoule breaker , Cat# 60600 for $12. We've used them for
breaking ampoules of paraformaldehyde, glutaraldehyde, acrolein and osmium
within seconds. The soft silicon molds can be twisted to achieve a quick
break. I usually tap the osmium tetroxide crystals to the bottom and use
forceps to lift the open ampoule into my prepared 50 ml of distilled water
to make our stock 2%. I have no financial interest in this company but am
a satisfied customer for many years. Phone number is: (800) 523-5874.
Rosemary Walsh,
EMF for the Life Sciences
PSU



From daemon Fri Sep 7 16:43:16 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 7 Sep 2001 16:39:49 -0500
Subject: Wiggl vs. waggle

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Fred:
Have you given any though to the fact that Coriolis' forces are
different in the southern hemispher? Water drains from a basin in a
counterclockwise motion south of the Equator and clockwise north of the
Equator. Maybe wiggles and waggles are affected thesame way between the
eastern and western hemispheres.

Regards,

Sam Purdy



Technical Center
National Steel Corp.
1745 Fritz Dr.
Trenton MI
Voice:734-676-2682
FAX: 734-676-2682
spurdy-at-nationalsteel.com


From daemon Fri Sep 7 17:28:59 2001



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Fri, 7 Sep 2001 18:25:23 -0400
Subject: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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Use a triangular metal file to score around the neck, then use the
usual precautions (protect fingers, open in hood , etc.) to pop open the
vial. The EMS brand I use breaks open easily by wrapping paper towel/ gauze
around top and gentle application of pressure.


Becky Garrison
I am not an employee of any EM supplier.

-----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
Sent: Friday, September 07, 2001 5:31 AM
To: Monson, Frederick C.
Cc: 'Microscopy Listserver'


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the
refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no
untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass
or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 7 18:10:29 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 07 Sep 2001 19:04:54 -0500
Subject: Breaking osmium tetroxide ampoules

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
========================================================
While we are on the topic of making up fixatives I have a supplementary
question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a glass rod.
This is nerve-wracking, tedious (they do not break readily) and not as safe
a procedure as I would like.

The ampoules used to have a weakened neck which could be sawn off but they
do not come in this form from our supplier any more.

Is there an easier way?
==========================================================
I am a bit surprised to hear that you are getting osmium tetroxide that is
not in prescored ampoules. So far as I know, all of the major suppliers of
EM chemicals, like SPI, have long since supplied their ampoules in the pre-
scored form. The only time I have seen in recent years ampoules not in pre-
scored form are those that are destined for other markets and applications.

Also, SPI, as well as the other major suppliers have long offered ampoule
neck breakers, such as are described on URL
http://www.2spi.com/catalog/tools/smtol21.html
These are meant to be used one time and discarded. They are inexpensive and
seem to provide an extra element of safety.

Using only prescored ampoules and an ampoule neck breaker might be easier on
the nerves.....

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Sep 8 11:38:40 2001



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Tuesday, September 04, 2001 4:09 AM
Subject: TEM CM10 Help!

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Marco,

Below are the two answers to your question, since I am not sure whether you
have
obj. aperture inserted in the beam, or you don't. Both assuming that the
field of view that you mentioning is the image of the sample in the holder,
rather than an illumination circle.

1. If obj. apertures holder is inserted in the beam.
Objective aperture should not be used in LM range. In Philips TEMs starting
with EM400 series , the main optical configuration difference between LM and
M modes is very distinct and as followed: in LM mode, the objective lens
current is very low (few hundreds milliAmps, depending on HT set), and is
fixed- hence magnification of obj. lens is also low (between 1 and 10,
closer to 1- just a guess). Focusing in LM mode is done by diffraction lens.
Almost all magnification is accomplished by lenses below the objective lens.
Obj. aperture is located between the specimen and that lens system, much
closer to the specimen, than to the first magnifying (diffraction) lens, and
is blocking most of the image if inserted in the beam.

In M mode, the obj. lens current is much higher (several Amps, depending on
the particular TEM model and the HT set), making magnification of the obj.
lens in M mode much higher (say, 50 or so). You can use obj. aperture now,
because of the significant part of the magnification happens very close to
the obj. aperture, plus much smaller area of the specimen is forming first
(objective lens) image. Focusing in M mode is done by the obj. lens.
Diffraction lens current in M mode is fixed and depends on the HT and
magnification set.

This two modes are "toggled" when TEM magnification is switched LM to M and
vice versa, in a single magnification step.

2. If obj. apertures holder is withdrawn from the beam.
When the condenser aperture is out of the beam, field of view in the lower
LM range increases. Nevertheless, obj. aperture holder shall not be visible
when withdrawn from the beam (control lever to the right). If obj. apertures
holder is still visible, then either the column alignment, or the obj.
aperture mechanical alignment (or both...) are incorrect.

If you are referring to illumination circle with the specimen holder
withdrawn- I wouldn't care much about obj. aperture holder visible on the
periphery of the illumination circle at the lowest LM steps, as long as it
is not blocking the sample image at the corresponding extreme of the sample
travel, and as long as everything else looks normal.

If you need to align the "out" position of any aperture holder on this TEM,
turn little Allen set screw located on the right side of the appropriate
aperture holder mechanism housing.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: "marienti-at-tiscalinet.it"-at-sparc5.microscopy.com
{"marienti-at-tiscalinet.it"-at-sparc5.microscopy.com}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Sun Sep 9 18:56:36 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Mon, 10 Sep 2001 09:09:57 +0930
Subject: Australian 2002 EM Conference

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Dear Microscopists,

How would you like to escape the northern winter this year?

Yes? Then join us for the 17th Australian Conference on Electron
Microscopy, to be held in Adelaide, South Australia, from February 4 -
8, 2002. Encompassing the areas of Electron, Scanned Probe and Confocal
Microscopies, this important meeting will provide an opportunity to
explore the many applications of these techniques, to keep abreast of
the latest developments and to meet and mix with many of Australia's and
the world's eminent leaders in the field.
This meeting was last held in Adelaide in 1988. A lot has changed in
this city in the past 14 years. Adelaide has emerged as a vital city
which can offer the visitor a range of exciting opportunities. The local
wine and food have attained international recognition. Tourists are
welcomed and well catered for. The conference will be held in the brand
new Adelaide Convention Centre which is conveniently located in the CBD,
close to accommodation and other facilities.
International visitors should take some extra time either before or
after the conference to explore Adelaide and South Australia. Tours can
be arranged to take you to the sights of Adelaide, the nearby
picturesque Adelaide Hills, and the wine growing regions of McLaren
Vale, the Barossa Valley and Clare Valley. Each of these areas has its
own unique history, heritage sites and gourmet delights! Adelaide's
clean white beaches are within easy reach of the city. Experience the
wildlife, flora and landscape of Kangaroo Island. Adelaide is also the
perfect stepping-off point to locations such as the beautiful Flinders
Ranges, where centuries-old Aboriginal art and ancient geological
formations can be seen, Uluru, the opal mining town of Coober Pedy, and
the Outback. The current exchange rates ensure that travel in Australia
has never been more economical for the overseas tourist.
A series of pre-conference workshops will be held to allow participants
to gain hands-on experience in the latest techniques and applications.
Many of the keynote speakers will participate as presenters, providing a
wonderful opportunity for interaction with these noted scientists. Be
sure to book early, as places in some of the workshops is limited.
During the week, delegates will have the chance to mingle and relax
after hours in several of Adelaide's noted heritage locations. A range
of accommodation choices, from five-star to budget, ensures choices
suitable for all.
With the deadline for abstract submission fast approaching, (October 6),
start making plans now to join us in February. Abstracts should be
submitted electronically, and the registration form can be downloaded
from the website. Full details can be obtained at
http://www.adelaide.edu.au/CEMMSA/acem17 Keep an eye on the website for
regular updates.
We look forward to seeing you at ACEM17.

ACEM17 Organising Committee


CEMMSA
Centre for Electron Microscopy and Microstructure Analysis
Adelaide University
Adelaide SA 5005
Ph: (08) 8303 4074
Fax: (08) 8303 4356
Website: http://www.adelaide.edu.au/CEMMSA


From daemon Sun Sep 9 19:03:01 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 10 Sep 2001 10:04:48 +1000
Subject: Re: Question about preparing osmium tetroxide

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At 10:30 7/09/01 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


we 1.
thouroughly clean off any label and label glue from the vial

2. put a scratch right around the vial

3. THEN it becomes easier to smash in the bottle, as you do it.

AND if you want, you can promote a crack around the line of the scratch by
touching a redhot glass rod to the scratch.

AND if you want, you can distribute the OsO4 in a thinner layer around the
wall of the vial by warming the (unscratched) vial in hot water, then
rolling the vial as the OsO4 cools.

This accelerates solution.......

AND if your OsO4 starts to go dark, you can refresh it almost completely by
adding hydrogen peroxide dropwise and thus titrate it to a clear solution
again. Oten you can restore it to straw colour, but we will use even a
pale purple solution as it will have enough tetroxide in it to work.






From daemon Sun Sep 9 20:13:36 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Sun, 9 Sep 2001 21:11:32 -0400
Subject: Re: Question about preparing osmium tetroxide

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Upon reading subsequent posts about breaking OsO4 vials, I realized I must
amend my suggestion of wrapping the neck with paper towels and gently
applying pressure to break at the neck. This method works fine on
PRE-SCORED vials, but almost certainly is unsafe or ineffective when used
on unscored vials. I wasn't aware that unscored vials were still being
sold.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Sun Sep 9 20:56:06 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Mon, 10 Sep 2001 11:55:32 +1000
Subject: Re: Electron Microscopes: Electric Trains, Buses, Subway

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Hi John,

I have an Hitachi H7100FA TEM on the second floor of a building built
almost directly over an underground electric railway. When I moved
in, bringing the EM with me, there was the choice of various
locations. I spent very little time standing on a wooden floor
feeling it shake under me. I spent considerably more time sitting on
a concrete floor with a saucer of water watching for vibrations (we
had no money to pay experts). Apart from having someone ask me if I
needed medical help, I was left in peace with my saucer and am now
quite happy with the chosen location. I don't work at REALLY high
mags, but the resolution test was accomplished without any vibrations
and I've coexisted happily with the trains for 3 years. Before that I
was on the 2nd floor of a concrete building next to a busy road and
it was dreadful. The building construction and location within it was
the important aspect for me.

Diana

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Mon Sep 10 00:56:38 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 10 Sep 2001 15:55:51 +1000
Subject: Oxford ISIS computer

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Dear Listers,

We are currently running an Oxford ISIS EDS system and an Oxford MonoCL
cathodoluminescence system from the same computer and on the same SEM. We
want to split the Cathodoluminescence system off onto another column so we
need another ISIS board enclosure (box).

We would be pleased to hear from anyone upgrading their system who would
have an ISIS box that is spare. (The box that houses the ISIS boards, not
the PC.) We do not specially need the ISIS boards.

We will pay a reasonable amount (say US$5,000) plus shipping for an ISIS
box in working order.




Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Mon Sep 10 02:10:34 2001



From: Alistair Smith :      A.Smith-at-macaulay.ac.uk
Date: Mon, 10 Sep 2001 08:06:21 +0100
Subject: unsubscribe

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Alistair Smith
Head of Analytical Group
The Macaulay Institute
Craigiebuckler
Aberdeen AB15 8QH
Scotland UK

Tel: +44(0) 1224 498228
Fax: +44(0) 1224 498208

e-mail: a.smith-at-macaulay.ac.uk

Web sites:
http://www.macaulay.ac.uk
http://www.macaulay.ac.uk/analytc.htm




From daemon Mon Sep 10 06:25:39 2001



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Mon, 10 Sep 2001 13:16:15 +0200
Subject: Epoxy question

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Dear microscopists

I'd like to prepare catalyst particles for TEM viewing by ultramicrotomy.
The problem is that I want to study the location and nature of carbon in
these particles and I am not certain how to distinguish the carbon from the
epoxy. We currently use Spurr's resin, but is there an epoxy I can use that
is not carbon based ?

TIA
Willem Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 960 - 4211/2772
Fax : +27 +16 960 - 2826
E-mail : willem.erasmus-at-sasol.com
PO Box 1, Sasolburg, 1947, Republic of South Africa

[All views expressed are my own and not necessarily that of my employer.]




From daemon Mon Sep 10 07:11:35 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Mon, 10 Sep 2001 22:05:26 +1000
Subject: Fw: Carnoy 1.0: scientific imaging for Mac OS X and Windows

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Could be interesting for you...
----- Original Message -----
} From: "Steven Dessein" {steven.dessein-at-BIO.KULEUVEN.AC.BE}
Dear Taxacomers,

The Laboratory of Plant Systematics (K.U.Leuven, Belgium) today released
Carnoy. Carnoy is an easy-to-use image analysis program, designed to carry
out measurements on digital images and especially on micrographs obtained
from light or electron microscopes.

Carnoy features a unique one-click calibration option: click on the scale
bar, enter its length and you are ready to carry out real-world measurements
on your micrographs.
Carnoy measures length and surface area of regular and irregular objects. It
also performs automated particle analysis (counting and measuring). The
program can handle BMP, PICT, Photoshop, JPEG, GIF, PNG, SGI, TGA, TIFF and
QuickTime image files and can convert between these file types.
Measurements are stored in a list that smartly manages all data. Every
measurement can be annotated. Measurements can be exported to a
tab-delimited file, which can be read by any text editor, word processor,
database or spreadsheet, for further analysis.
The program's clean and easy-to-use interface enables you to be productive
within minutes.

Carnoy can be used in the following fields: taxonomy, morphology, ecology,
physiology, molecular cell biology, agricultural sciences, paleontology,
archaeology, among others.
Carnoy requires a Macintosh running Mac OS X or a PC with at least 64 MB RAM
and Windows 95 / 98 / ME / NT / 2000 / XP. A resolution of 1024 x 768 pixels
(or higher) is recommended.

Carnoy is $15 shareware (if you cannot afford this, please, contact
peter.schols-at-bio.kuleuven.ac.be). It is available for download on the Carnoy
website.
http://www.carnoy.org

Kind regards,


P. Schols, S. Dessein & E. Smets


Steven Dessein
Laboratory of Plant Systematics
Institute of Botany and Microbiology
Kardinaal Mercierlaan 92
B-3001 Leuven
Belgium

tel. ++ 32 16 321536
fax. ++ 32 16 321968

http://www.kuleuven.ac.be/bio/sys
http://www.kuleuven.ac.be/bio/sys/mactaxon

e-mail: steven.dessein-at-bio.kuleuven.ac.be




From daemon Mon Sep 10 07:42:24 2001



From: pmoore-at-wfubmc.edu (Paula Moore)
Date: Mon, 10 Sep 2001 08:33:25 -0400
Subject: Re: Breaking osmium tetroxide ampoules

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Folks,

I'm not sure if our Osmium vials are prescored or not, but we've never had any
problems or the nerve wracking experiences that you have mentioned.
We score around the vial a couple of times with a diamond pen(made especially
for marking on glass). The side where we want the break is scored a couple
times more. In the fume hood we wrap the vial in a paper towel and snap it
facing toward the back of the hood.
Hope this helps.

Paula Moore
Wake Forest University School of Medicine
Pathology/EM Lab
pmoore-at-wfubmc.edu


}
} How do you open glass osmium tetroxide ampoules?
}
} I put mine in a 100ml glass bottle and then break it with a glass rod.
} This is nerve-wracking, tedious (they do not break readily) and not as safe
} a procedure as I would like.
}
} The ampoules used to have a weakened neck which could be sawn off but they
} do not come in this form from our supplier any more.
}
} Is there an easier way?
} ==========================================================
}
}
} Using only prescored ampoules and an ampoule neck breaker might be easier on
} the nerves.....
}
} Chuck
}
} ==================================================



From daemon Mon Sep 10 08:56:17 2001



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Mon, 10 Sep 2001 08:49:20 -0500
Subject: SEM available

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Amray 1610 SEM with PGT EDS free to a good home---interested party pays all
shipping charges. Please send queries off-list.
Thanks!

Melissa Troost
Electron Probe Instrumentation Center
Northwestern University
Evanston, Illinois, USA
847-491-3439
m-troost-at-northwestern.edu


________________________________
Legal Notice: This message is confidential is intended for the stated
addressee(s) only.
Access to this e-mail by anyone else is unauthorized. If you are not the
intended recipient, please inform the sender immediately.



From daemon Mon Sep 10 08:58:52 2001



From: Mark Germani :      mgermani-at-micromaterialsresearch.com
Date: Mon, 10 Sep 2001 08:56:39 -0500
Subject: SEM photos of microbiologicals

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Does anyone have or know of a source of SEM photomicrographs of
bacteria, mold and yeast. I am asking on behalf of a colleague who will
pay a royalty for the use of the photos.


Mark Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive, #200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 fax

mgermani-at-micromaterialsresearch.com




From daemon Mon Sep 10 12:26:26 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Mon, 10 Sep 2001 13:17:49 -0400
Subject: Re: TEM of x-ray film

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Anyone out there knows how to run up an X-ray film (exposed as well as
unexposed) for TEM?????


Dear Neelima,
What do you mean by "run up"? We are lucky to have our LoDose and
MRF32 x-ray films cut to the appropriate size--6.5 x 9 cm--and we treat
them like other EM film EXCEPT, we load and develop them in total darkness,
and we use x-ray film developer in the tank instead of D-19. Our darkroom
is in the same room as the scope, so transport can occur in total darkness.
If this is not the case for your facility, and you have to run the film up
to your darkroom, get a film transport bag from a photo supply store, put
the film in its original box, put the box in the bag, and carry it to your
darkroom. The bags are designed to be light tight. Good luck, and if you
mean something else by "run up", let the list know, and I'll try to give
you a better answer.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Mon Sep 10 13:51:57 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Mon, 10 Sep 2001 15:03:42 -0400
Subject: RE: Ethanol vs. Acetone for critical point drying

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Karen:
Stick to absolute ethyl alcohol (Et0H). Never use acetone with any critical
point drying apparatus that have glass viewing windows held in place with a
epoxy resin that would be attacked by acetone.
The white crystals may very well be buffer salts. You can start dehydrating
with a larger ratio of water to ethyl alcohol and let stand for a longer
time to remove buffer salts while working up to absolute strength ethanol.
For some samples such as tissue culture monolayers the critical point
dryers can be avoided altogether by proceeding from the absolute ethyl
alcohol to 1,1,1,3,3,3-hexamethyldisilazane to air dry. I would recommend
the 99.9% pure solution.

Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
State University of New York-Binghamton
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

} ---Original Message------------------
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Mon Sep 10 14:49:28 2001



From: yan.hu :      yan.hu-at-brunel.ac.uk
Date: Tue, 11 Sep 2001 02:35:37 -0500
Subject: ask help

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Dear Listers,

I got a problem when preparing TEM foils of Al-alloy by means of
electro-polishing. A dark film produced on the specimen surface when it
was been polishing. I think, the polishing condition is right and
listed below:

Solution, 25 % Nitric acid plus 75 % Mechenol
Temperature, about -30 degree C
Voltage, 15-20V

I would be pleased to hear from anyone who has an idea to sort out this
problem.


Regards

Yan
----------------------

yan.hu-at-brunel.ac.uk


From daemon Mon Sep 10 14:49:30 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Tue, 11 Sep 2001 02:36:21 -0500
Subject: Re: Drosophila brain fixation

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Dear Randy,
Drosophila brains are very hard to fix. The neurons are very
delicate and lose their structure when not well preserved. What age
are the bugs? You might try some adding some acrolein (we used 1%
acrolein and 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.1)
and leave out the paraformaldehyde. See if you still have
immuno-reactivity You don't need to use propylene oxide. You can
use 100% EtOH instead. It is miscible with resins, as long as it is
totally dry. Also, don't store your specimens in buffer, but process
straight through. I do microwaving these days, so time is never an
issue anymore. Good luck!


JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856

04:18 PM 09/06/2001 -0500, you wrote:

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From daemon Mon Sep 10 16:29:20 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Mon, 10 Sep 2001 14:20:25 -0700
Subject: Announcement

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In conjunction with the Pacific Northwest Microscopy Society,
The University of Washington Core for Communication Research, Center on
Human Development and Disability and the W. M. Keck Center for Advanced
Studies in Neural Signaling present:

Microscopy and Digital Imaging: Advances & Applications

September 27, 2001
12:30 - 5 p.m.
Location: CD 150

12:30 "Multiphoton and Multispectral Imaging in Vertebrates"
Mary Dickinson, Caltech

1:15 "Some New Approaches to Intracellular Imaging"
Bob Fern, University of Washington

2:00 FRET imaging with the confocal microscope
Kolja Wawrowsky, Leica

2:45 Break

3:15 "In Vivo Microscopy of Murine Brain"
Liz Yoder, UCSD

4:00 "Deconvolution Methods in Wide-field and Confocal Microscopy"
Paul Goodwin, Applied Precision Inc

5:00 Reception

The Veteran's Administration Hospital and Medical Center Morphological
Analysis Core Facility will have an open house and talks on September
28, 2001.

Speakers sponsored by Applied Precision, BioRad, Leica, and Zeiss
The reception is sponsored by Bartels and Stout, Inc.

Contact Paulette Brunner pbrunner-at-u.washington.edu or Glen MacDonald
glenmac-at-u.washington.edu for information.

Regards,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************

C:} The box said "Requires Windows95 or better". So I bought a
Macintosh.
**************************************************************************





From daemon Mon Sep 10 17:27:38 2001



From: sbcook-at-bcpl.net ()
Date: Tue, 11 Sep 2001 05:22:19 -0500
Subject: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sbcook-at-bcpl.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
September 9, 2001 at 22:52:34
---------------------------------------------------------------------------

Email: sbcook-at-bcpl.net
Name: Katelyn Cook

Organization: St. Michael the Archangel

Education: 6-8th Grade Middle School

Location: Baltimore, MD

Question: I am planning to do a science fair project about germs and
the importance of hand washing. I want to collect germs from common
public places such as doorknobs, handrails, telephones, sink faucets,
etc. What is the best way to collect the germs? Will I need to grow
the germs in a petri dish after I collect them? Will I be able to
identify different types of germs using my microscope?

---------------------------------------------------------------------------


From daemon Mon Sep 10 17:40:42 2001



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Mon, 10 Sep 2001 15:35:20 -0700
Subject: Re: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen cellular voids from poor fixation as well as from genetic
mutations. The biggest impediment I have when fixing drosophila brains is
getting the fixative to the tissue. I recommend cutting the neck as well as
the proboscis and then using gentle centrifugation (microcentrifuge
~2000rpm for one minute) to get the heads to sink. Due to air in the brain
cavity this last step does not always work. If you use 2% glutaraldehyde
and osmium post fixation do you get good ultrastructure? In a project I did
some time ago I had the best results using 0.07 M sodium cacodylate buffer
at pH 7.5. That gives an osmolarity of about 187mOsm. However, I routinely
use 0.1 M sodium cacodylate and find that adequate.

At 04:18 PM 9/6/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Sep 10 18:15:45 2001



From: Ubirajara Pereira Rodrigues Filho :      uprf-at-iqsc.sc.usp.br
Date: Mon, 10 Sep 2001 20:11:26 -0300
Subject: SEM of tempers in potteries ( specially shells )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Liststers;
}
} I'm working with SEM of pre-colonial potteries from Pantanal at the =
} Brazilian West border with Bolivia. In some samples we've found shell =
} and sponge spicule in the bulk of potteries. We would appreciate any =
} article or book with micrographies of similar materials in pottery ? =
} Also, if someone could provide me references on clay with sponge =
} spicule, would be very helpful.
}
} Ubirajara Pereira Rodrigues-Filho
Instituto de Química de São Carlos
Universidade de São Paulo
São Carlos, Brazil



From daemon Mon Sep 10 18:40:15 2001



From: Corvos-at-aol.com
Date: Mon, 10 Sep 2001 18:34:50 -0500
Subject: Re: Cameca MBX Microprobe is available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

Cameca MBX Microprobe is available at this time.
4 wavelength spectrometers (includes PC2 crystal for Lt ele. spec.)
Kevex Sigma EDS
New GW Backscattered system
PC Based operating system
Diff pump vacuum system
Cathodoluminescence system (OEM)
System is about 17 years old and in good condition (at last service call).
Has been turned off for 1 year.

Please send inquires to E-MAC
Walter Protheroe
corvos-at-aol.com

All inquires will be passed along.


From daemon Mon Sep 10 18:47:36 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 10 Sep 2001 16:39:59 -0700
Subject: Re: SEM photos of microbiologicals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark:

You may want to try the following website at Utah State University:

http://bioweb.usu.edu/emlab/Galleries.html

They have many great SEM images. A contact person there to talk to
would
be:

Bill McManus
TEL: (435) 797-1920

I hope this helps.

Best regards-

David

Mark Germani wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Does anyone have or know of a source of SEM photomicrographs of
} bacteria, mold and yeast. I am asking on behalf of a colleague who will
} pay a royalty for the use of the photos.
}
} Mark Germani, Ph.D.
} MicroMaterials Research, Inc.
} 136 Shore Drive, #200
} Burr Ridge, IL 60521
}
} (630) 325-8170
} (630) 325-8178 fax
}
} mgermani-at-micromaterialsresearch.com

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Mon Sep 10 18:55:51 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Mon, 10 Sep 2001 18:50:17 -0500
Subject: Re: Drosophila brain fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,
Drosophila brains are very hard to fix. The neurons are very
delicate and lose their structure when not well preserved. What age
are the bugs? You might try some adding some acrolein (we used 1%
acrolein and 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.1)
and leave out the paraformaldehyde. See if you still have
immuno-reactivity You don't need to use propylene oxide. You can
use 100% EtOH instead. It is miscible with resins, as long as it is
totally dry. Also, don't store your specimens in buffer, but process
straight through. I do microwaving these days, so time is never an
issue anymore. Good luck!


JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856

04:18 PM 09/06/2001 -0500, you wrote:

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Hello everyone,
I have a researcher here that is having some difficulty with getting good
histology at the EM level. She is fixing Drosophlia brains with 4%para/0.5%
glut in phosphate buffer pH7.2, no osmium post fix, ethanol dehaydration,
propylene oxide as a transitional solvent to polybed 812, then performing post
sectioning immunolabeling. The tissue has "holes" in it when viewed at the
TEM. They are not holes in the section, but appear to be voids in the tissue.
We have tried leaving out the propylene oxide, we have osmicated, and have
looked at osmotic strength of the fixative (and many variations of the above,
to no avail). Can anyone offer advice?
Randy Nessler
Univ. of Iowa


From daemon Mon Sep 10 20:27:05 2001



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Mon, 10 Sep 2001 19:16:43 -0600
Subject: RE: ask help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yan,
Assuming (sorry for that) that the polishing conditions are correct, and you
are producing well dished samples with thin area and the only problem is
sludge on the surface, try two things before changing any other conditions
and try them one at a time so as not to confuse the issue.
1: Try increasing the jet speed. If this doesn't work.
2: Try increasing the temperature about 5 degs.
It would be helpful to know what type of jet thinner you are using and
exactly what the alloy is.
Good Luck!
Dorrance

} ----------
} From: yan.hu
} Sent: Tuesday, September 11, 2001 12:35 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: ask help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} I got a problem when preparing TEM foils of Al-alloy by means of
} electro-polishing. A dark film produced on the specimen surface when it
} was been polishing. I think, the polishing condition is right and
} listed below:
}
} Solution, 25 % Nitric acid plus 75 % Mechenol
} Temperature, about -30 degree C
} Voltage, 15-20V
}
} I would be pleased to hear from anyone who has an idea to sort out this
} problem.
}
}
} Regards
}
} Yan
} ----------------------
}
} yan.hu-at-brunel.ac.uk
}
}
}



From daemon Tue Sep 11 00:14:03 2001



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Mon, 10 Sep 2001 22:07:06 -0700 (PDT)
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Tue, 11 Sep 2001 05:22:19 -0500, sbcook-at-bcpl.net wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Below is the result of your feedback form (NJZFM-ultra-55). It was
| submitted by (sbcook-at-bcpl.net) from
| http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
| September 9, 2001 at 22:52:34
|
---------------------------------------------------------------------------
|
| Email: sbcook-at-bcpl.net
| Name: Katelyn Cook
|
| Organization: St. Michael the Archangel
|
| Education: 6-8th Grade Middle School
|
| Location: Baltimore, MD
|
| Question: I am planning to do a science fair project about germs and
| the importance of hand washing. I want to collect germs from common
| public places such as doorknobs, handrails, telephones, sink faucets,
| etc. What is the best way to collect the germs? Will I need to grow
| the germs in a petri dish after I collect them? Will I be able to
| identify different types of germs using my microscope?
|
|
---------------------------------------------------------------------------
|

Dear Katelyn Cook -
While I was teaching as a graduate teaching assistant for a general
biology class, I had the opportunity to lead a group of non-
science college students on an examination of the microbial world around
them. What I did was to have each of them use a sterile, cotton-
tipped swab, moisten it a bit with sterile water (perhaps boiled water will
do just as well), swab the surface of whatever object they wanted to
explore, and then swab it onto the surface of a petri plate that already had
bacterial medium. They then left all the plates for me to seal and
incubate at room temperature for several days (or, at most, 1 week). When
they got the plates back, they then could see with the naked eye
the various colonies that formed and their various colors. If a microscope
is available, then it is possible to identify individual bacteria by using
a "wet mount" (that is, a slide with a small drop of sterile water and a
very small amount of whatever colony is chosen).
Although I don't have any experience with grade school students, I believe
that my approach could work. So... to answer your
questions: (1) A very easy way to collect bacteria is to first get some
sterile swabs. The fact that they are sterile is important because
any box of ordinary (or household) swabs is likely to already have bacteria
on them, and obviously that would defeat the purpose of your
experiment. As a result, you'll need some sterile swabs -- quite likely,
they can be bought at a pharmacy near you when you look for dressings,
bandages, etc. (2) Assuming you have sterile swabs, you could conceivably
boil some water, let it cool to room temperature, and let each
student lightly wet a swab (or more as needed). Why boil the water? For the
same reason as needing sterile swabs -- because ordinary tap
water will very likely contain bacteria (even if it looks perfectly clear).
One alternative is to use distilled water if boiling isn't feasible.
(3) Yes, you will need a petri plate -- the plate needs to have some kind of
bacterial medium capable of supporting their growth; otherwise,
your swabbing exercise will be fruitless. I do not know where such plates
can be obtained, though, and you may want to seek others on
this listserver; finally, (4) The use of a microscope is needed only if
you're interested in examining individual cells. The typical colonies that
are seen on the surface of a plate tend to vary in terms of both color and
the type of colony produced. That is, a colony could be white or
yellow, but its 'type" could be of a sticky nature or a more "liquid-like"
nature. That part could be revealing on its own, or perhaps not.
If you feel that a microscope is needed, then a simple way to see individual
cells is to simply add a small drop of boiled / sterile water onto a
slide, use an inoculating loop or needle, and gently pick up a small amount
of a colony, and spread it around within to the drop. Place a coverslip
over the slide, and put it onto the stage of a microscope. Try to use the
highest objective lens magnification that is possible -- if there are a 10X,
20X and
100X objectives, use the 20X as the individual cells are very small in size
-- and, if it's possible, try to set the microscope under non-brightfield
light conditions such as phase-contrast. Bacterial cells are, generally,
transparent to light, and as a result are often very hard to see because
they have very little contrast with the background light.
Anyway .. I hope you have some success with your experiment for these
grade-school students. It is my my belief that microscopic, living
things (such as the "germs" and, more specifically, bacteria, algae, etc.)
are always fascinating things for kids to see, and I hope it turns out
very well.
Good luck !
Nelson Conti
Former graduate student at San Francisco State University
(in San Francisco, CA)
A Bachelor's and Master's degree in Microbiology





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/




From daemon Tue Sep 11 02:13:28 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 11 Sep 2001 08:14:32 +0100 (GMT Daylight Time)
Subject: Re: Oxford ISIS computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Mel,

Have you contacted Oxford Instruments? With the INCA doing
as well as they tell me it is they must be taking in quite
a few ISIS towers on upgrades (they've had one of ours).
Of course if you expect a full manufacturer's warrantee it
might be pricey but if you took the same 'as seen'
conditions that you would accept from another user then
they might deal.

Regards,
Ron

On Mon, 10 Sep 2001 15:55:51 +1000 Mel Dickson
{m.dickson-at-unsw.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} We are currently running an Oxford ISIS EDS system and an Oxford MonoCL
} cathodoluminescence system from the same computer and on the same SEM. We
} want to split the Cathodoluminescence system off onto another column so we
} need another ISIS board enclosure (box).
}
} We would be pleased to hear from anyone upgrading their system who would
} have an ISIS box that is spare. (The box that houses the ISIS boards, not
} the PC.) We do not specially need the ISIS boards.
}
} We will pay a reasonable amount (say US$5,000) plus shipping for an ISIS
} box in working order.
}
}
}
}
} Dr. Mel Dickson,
} Deputy Director, The Electron Microscope Unit,
} Adjunct Associate Professor, School of Microbiology & Immunology
} The University of New South Wales
} UNSW SYDNEY 2052
} Australia.
} Phone +612 9385 6383 Fax +612 9385 6400
} http://srv.emunit.unsw.edu.au/
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Sep 11 02:38:08 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Fri, 07 Sep 2001 17:35:51 -0400
Subject: Re: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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EMS sells an ampoule breaker , Cat# 60600 for $12. We've used them for
breaking ampoules of paraformaldehyde, glutaraldehyde, acrolein and osmium
within seconds. The soft silicon molds can be twisted to achieve a quick
break. I usually tap the osmium tetroxide crystals to the bottom and use
forceps to lift the open ampoule into my prepared 50 ml of distilled water
to make our stock 2%. I have no financial interest in this company but am
a satisfied customer for many years. Phone number is: (800) 523-5874.
Rosemary Walsh,
EMF for the Life Sciences
PSU




From daemon Tue Sep 11 02:38:23 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 07 Sep 2001 19:04:54 -0500
Subject: Breaking osmium tetroxide ampoules

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Patton wrote:
========================================================
While we are on the topic of making up fixatives I have a supplementary
question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a glass rod.
This is nerve-wracking, tedious (they do not break readily) and not as safe
a procedure as I would like.

The ampoules used to have a weakened neck which could be sawn off but they
do not come in this form from our supplier any more.

Is there an easier way?
==========================================================
I am a bit surprised to hear that you are getting osmium tetroxide that is
not in prescored ampoules. So far as I know, all of the major suppliers of
EM chemicals, like SPI, have long since supplied their ampoules in the pre-
scored form. The only time I have seen in recent years ampoules not in pre-
scored form are those that are destined for other markets and applications.

Also, SPI, as well as the other major suppliers have long offered ampoule
neck breakers, such as are described on URL
http://www.2spi.com/catalog/tools/smtol21.html
These are meant to be used one time and discarded. They are inexpensive and
seem to provide an extra element of safety.

Using only prescored ampoules and an ampoule neck breaker might be easier on
the nerves.....

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From daemon Tue Sep 11 02:38:25 2001



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Fri, 7 Sep 2001 18:25:23 -0400
Subject: Question about preparing osmium tetroxide

Contents Retrieved from Microscopy Listserver Archives
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Use a triangular metal file to score around the neck, then use the
usual precautions (protect fingers, open in hood , etc.) to pop open the
vial. The EMS brand I use breaks open easily by wrapping paper towel/ gauze
around top and gentle application of pressure.


Becky Garrison
I am not an employee of any EM supplier.

-----Original Message-----
} From: Patton, David [mailto:David.Patton-at-uwe.ac.uk]
Sent: Friday, September 07, 2001 5:31 AM
To: Monson, Frederick C.
Cc: 'Microscopy Listserver'


While we are on the topic of making up fixatives I have a
supplementary question.

How do you open glass osmium tetroxide ampoules?

I put mine in a 100ml glass bottle and then break it with a
glass rod. This is nerve-wracking, tedious (they do not
break readily) and not as safe a procedure as I would like.

The ampoules used to have a weakened neck which could be
sawn off but they do not come in this form from our
supplier any more.

Is there an easier way?

Dave


On Thu, 6 Sep 2001 15:46:24 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I dissolve 1g OsO4 in 25ml double distilled water and store it in a bottle
} with a Teflon lined screw cap. The bottle is then held in the
refrigerator
} in a small plastic jar (available from all lab distributors - mine from
} Fisher or S/P) with a screw cap . In this way, the plastic becomes
} osmicated NOT the refrigerator. I have done this for years with no
untoward
} results.
}
} I fix with aldehydes in screw cap scintillation vials (20ml or 5ml) glass
or
} plastic. Osmication is done in glass vials with Teflon lined screw caps
} using a minimum of OsO4 to do the job.
}
} I make glutaraldehyde from a 10ml vial in dd HOH (50%, 10ml Glu + 40ml dd
} HOH yields 10% Glu) as a 10% solution. This in a screw top bottle with a
} Teflon lined cap. I then make up an appropriate amount of fixative using
} the 10% glu and 10X buffer concentrate where possible. The 10% Glu is
kept
} in a plastic jar in the refrigerator.
}
} I find that double containers are safe and reduce contamination
} considerably.
}
} Hope this helps.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Tue Sep 11 02:38:26 2001



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 7 Sep 2001 16:39:49 -0500
Subject: Wiggl vs. waggle

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Fred:
Have you given any though to the fact that Coriolis' forces are
different in the southern hemispher? Water drains from a basin in a
counterclockwise motion south of the Equator and clockwise north of the
Equator. Maybe wiggles and waggles are affected thesame way between the
eastern and western hemispheres.

Regards,

Sam Purdy



Technical Center
National Steel Corp.
1745 Fritz Dr.
Trenton MI
Voice:734-676-2682
FAX: 734-676-2682
spurdy-at-nationalsteel.com



From daemon Tue Sep 11 02:38:28 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 7 Sep 2001 14:45:06 -0400
Subject: Re: Ethanol vs. Acetone for critical point drying

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Karen:

Another source that we have come across is sodium phosphate buffer
crystals. If fixation is carried out with sodium phosphate buffered soluitons,
and then immediately transittioned to dehydration solvent (either EtOH or
Acetone) then we have seen samples coated with fine crystals. We use
either serveral water rinses between fixation and dehydration or an alternative
buffer (i.e. Na Cacodylate)

}
} Recently, a collegue was having problems with a white crystaline
} substance covering his samples of ear tissue after they were removed
} from the critical point dryer. It was believed to be from a
} contaminant in the ethanol and it was recommended to switch to acetone
} dehydration as it was less likely to have this problem. I haven't done
} SEM in years, so I haven't been paying attention to anything like this
} on this listserve. I was wondering if other people experiencing this
} problem. If you are using acetone, have you ever seen this?
}
} Thanks for your comments,
}
} Karen Pawlowski, Ph.D.
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Tue Sep 11 02:38:25 2001



From: Nancy Zjaba :      nzjaba-at-alfalight.com
Date: Fri, 7 Sep 2001 15:54:34 -0500
Subject: Thanks for tilt correction factor

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Thanks to all who responded to this question. The most often-cited equation
was:

Real length = Observed length/cosine (tilt angle)

Several people noted that sample geometry plays a role, and that this
equation is useful only for flat samples.

Have a good weekend, everyone.

Nancy Zjaba, Technical Staff
Alfalight Inc.
1832 Wright Street
Madison, WI 53704
(608) 240-4875
nzjaba-at-alfalight.com




From daemon Tue Sep 11 02:38:28 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 7 Sep 2001 17:05:33 -0400
Subject: RE: Bio. Fixation: Which Champy's?

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Gray, Peter, 1975, Microtomist's Formulary and Guide, R. Krieger,
Huntington, NY. (Still available for the hardy bibliophile at
http://WWW.WEB4U.COM/cgi-bin/full_page?0-88275-247-2) reports FOUR fixative
mixtures that are attributed directly to Champy:

1. Sat. aq. phenol(200ml)+40%HCHO(48ml)+Trichloroacetic
acid(3.6g)

2. HOH(20)+2% CrO3(50)+pyrolignious acid (35)(URL:
http://www.ayrshirehistory.org.uk/AcidWorks/acidworks.htm#Pyroligneous%20Aci
d)

3. HOH(33)+2% OsO4(22)+2%CrO3(20)+7.5% K2Cr2O7(16)

4. HOH(60)+7.5% K2Cr2O7(40)

My favorite, of course, is number 2!!! whose last component provides a
wonderful bit of chemical history. We see how Bayer must have started.
Dupont.

Regards, and I know that this doesn't help at all!

But it is all part of the art.

Fred Monson









} ----------
} From: Richard Edelmann
} Reply To: edelmare-at-muohio.edu
} Sent: Wednesday, September 5, 2001 4:24 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Bio. Fixation: What is Champy's Fluid?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} "Fixed 2-5 minutes in Champy's fluid, rinsed 2x in water" . . . followed
} by
} ETOH dehydration, CPD, mounting and SEM viewing.
}
}
} O.k., we give up, searched numberous books, papers, catalogs, web
} sites, etc. . . . does anyone have a recipe for "Chmapy's fluid" a.k.a.
} "Champy's Solution"? Please?
}
} Thank you much!
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From daemon Tue Sep 11 02:45:27 2001



From: Macatangay, Peggy J., Celanese/US :      PJMckarns-at-celanese.com
Date: Thu, 23 Aug 2001 13:13:47 -0500
Subject: SEM-EDS: Help with choosing a new system

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I am investigating EDS systems to integrate with a JEOL-6100 SEM. I'm
leaning towards PGT's Spirit system. Other considerations are Oxford's INCA
and Thermo Noran's Vantage. Does anyone have particularly positive or
negative experience with PGT? I don't often hear much about that company.
Any comments about the other possibilities would be appreciated, as well.

Thanks!

-Peggy

Peggy McKarns Macatangay, PhD
Project Analyst 2
Celanese Corpus Christi Technical Center




From daemon Tue Sep 11 02:45:27 2001



From: john_bruss-at-bose.com ()
Date: Thu, 23 Aug 2001 13:45:37 -0500
Subject: Ask-A-Microscopist: how to suspend a spider in a clear cube

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (john_bruss-at-bose.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
August 22, 2001 at 17:00:52
---------------------------------------------------------------------------

Email: john_bruss-at-bose.com
Name: John Bruss

Organization: Bose Corp.

Education: Graduate College

Location: San Diego, CA, USA

Question: What materials and process would an amateur use to suspend
a spider in a clear cube for unmagnified artistic and historic use?
Where are those materials available in small quantities?

---------------------------------------------------------------------------



From daemon Tue Sep 11 02:46:21 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 23 Aug 2001 12:32:20 -0500
Subject: RE: Spectral Imaging with ISIS

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I am pretty sure that Alwyn is not talking about x-ray mapping. If that was
all he wanted, I suppose the key disk is all that would be needed if he
already has the other imaging applications and hardware installed.

Someone suggested using Auto to scan an image for multiple spectra. Indeed,
the software and hardware should allow it. The spectra might then be
exported and processed by some other package to perform the type of
component analysis that has been described at MSA over the last few years.
My current ISIS x-ray application (version 3.32) has the option of storing
the x-ray data in single column MSA format. Previous versions placed
multiple channels on a single line.

One hitch would be the limitation on the number of spectra per job. I think
there is a limit of 1000 or so built into the database used for managing
the data. I suppose it could be changed to another number, but we bumped
into it some time back.

That would mean you could do a 32x32 raster of points saving a spectrum at
each. A four second acquisition per point would mean a bit more than an
hour for acquisition. That would not be too bad, but the spatial resolution
would probably be too low to be very useful. But the data should readily
fit onto hard drives with capacities of a few gigabytes.

I would be interested in seeing how the data processing is handled. If
someone makes it work, I would be very interested in hearing the details.

Warren

At 10:33 AM 8/23/2001 -0400, you wrote:
} -----------------------------------------------------------------------.
} This is correct, the keydisk is all that is required for
} the software to be enabled. However, there may be hardware
} required, too, at least a cable interface (RS232?) to the
} microscope. I believe the ISIS Autobeam likes to select
} its own scan rates. You may need to speak with Oxford re:
} compatibility with your particular microscope.
}
} Matt
}
} Matthew J. Lynn
} Center for Advanced Microscopy
} University of Miami
} (305)284-4736
} mlynn-at-miami.edu
}
}
} On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
} [SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
} }
} } Alwyn:
} }
} } By spectral images, do you mean xray mapping? A "key" disk for the ISIS
} } software program is required to activate the xray mapping facility. Of,
} } course this will cost a bit of money to purchase from Oxford. When the
} } ISIS program is loaded initially, all the software required to use the
} } program is there, but activating the unpaid for parts of the program is
} } the thing.
} }
} } Fred
} }
} } On Wed, 22 Aug 2001, Alwyn Eades wrote:
} } } We have an Oxford ISIS EDS system. As it stands it is not set up to
} } } acquire spectral images. We would like to be able to do spectral image
} } } acquisition on this machine. Is there anyone out there who knows how
} } } the system may be modified (macros, interfaces, whatever) to do it? We
} } } do have Digital Micrograph (on a Mac whereas ISIS is on a PC), if that
} } } helps.
} } } ..........
} } } Alwyn Eades
} } } Department of Materials Science and Engineering
} } } Lehigh University
} } } 5 East Packer Avenue
} } } Bethlehem
} } } Pennsylvania 18015-3195
} } } Phone 610 758 4231
} } } Fax 610 758 4244
} } } jae5-at-lehigh.edu




From daemon Tue Sep 11 02:49:04 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 25 Aug 2001 15:57:15 -0400
Subject: Re: Which is Correct, EDS or EDX?

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Mike,
My guess is that either will do the trick, but I would take EDX as a
shortened version of Energy Dispersive Analysis of X-rays, which, of
course, is EDAX. I've also seen EDXA which would keep you out of
trouble with EDAX. One really is doing a specific type of analysis,
spectroscopy, which would cause me to lean towards Energy Dispersive
Spectroscopy.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Ingram, Mike wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Not a serious question here, more of a curiosity.
}
} I use EDS when talking about x-ray analysis. I see many using EDX. Which
} is correct?
}
} Mike Ingram
}
}
}




From daemon Tue Sep 11 04:29:04 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Tue, 11 Sep 2001 10:20:48 +0000 (GMT)
Subject: Debye-Sherrer cameras

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Dear Listers,
In a clear out of a cupboard I have found a pair of Debye-Sherrer cameras and accessories. If anyone would like them, please contact me - otherwise they're skip fodder. I used to demo this as an undergraduate lab some years ago, but I guess no-one uses them any more?

Regards,

Richard Beanland

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
Marconi Optical Components Limited
Registered in England No. 4113798 Registered Office: One Bruton Street London
W1J 6AQ





From daemon Tue Sep 11 07:03:31 2001



From: Wang, Dashan :      Dashan.Wang-at-nrc.ca
Date: Tue, 11 Sep 2001 07:56:14 -0400
Subject: looking for chips

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Dear Listers,

The CCD camera (model 679) in our CM20 TEM does not work any more. It is
the fist commercial CCD camera of Gatan. The company is experiencing hard
time to find the right replacing part. A programmable chip ( TEK1B2FV9
made by ACTEL) for the camera controller is needed. Then they can possibly
figure out what problems with the camera.

If you have this part or any information which can help us to sole this
problem please let me know. I can be reached at (613) 990-1403 or
dashan.wang-at-nrc.ca

Thank you!

Dashan Wang


From daemon Tue Sep 11 07:05:00 2001



From: hagglund.kw-at-pg.com
Date: Tue, 11 Sep 2001 07:58:19 -0400
Subject: Re: SEM of tempers in potteries ( specially shells )

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Try Prudence Rice's book Pottery Analysis: A Sourcebook (1987). This was the
text on archaeological ceramics a few years back, and would be a good starting
point. It is available for $65 on amazon.com.

Karl Hagglund
(513) 634-0146



From daemon Tue Sep 11 07:55:58 2001



From: Jill Verlander Reed :      verlaj-at-medicine.ufl.edu
Date: Tue, 11 Sep 2001 08:44:59 -0500
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

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Dear Katelyn,
I will only add to Nelson Conti's remarks that you should be able to buy
"blood-agar plates," which are Petri dishes already filled with a
sterile, general bacterial culture medium, as well as sterile
cotton-tipped swabs, from a medical supply store in your area. My
daughter did a project along the same lines as yours and that's where we
got the supplies. The dishes came in packs of 10, I think and they only
cost a few dollars.
We kept the inoculated dishes in a warm place. Around body temperature
(37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
If you find bacterial colonies that have a clear ring around them,
instead of the red of the rest of the blood agar, that is an indication
that the bacteria in that colony are pathogenic (disease-producing).
Good luck, and have fun.

Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299


From daemon Tue Sep 11 08:53:16 2001



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Tue, 11 Sep 2001 08:50:44 -0700
Subject: Re: Electron Microscopes: Electric Trains, Buses, Subway

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John

At UIC we are less than 800m from the Blue Line of the cta L. When the lab
was set up we bought an EMF cancellation system, in case. However, it
turned out we did not need it despite doing high resolution annular dark
field STEM with sub 0.2nm resolution.

While I was working for VG we did install a HB501 STEM in Halle,
Germany. Here it was a different story. The main streetcar line through
the city ran less than 100m from the site and to make matters worse a main
feed for the system ran perpendicular to the line along a second wall of
the building (probably 50m away). You could see each and every streetcar
entering the stretch of line powered by the feed, it caused a sideways
image jump! This was fixed by putting a Hall effect coil in the room at
microscope bench level with a feed into the alignment coils.

If this is a new transit system then you are likely to suffer less problems
with vibration and magnetic fields than you are with an old system where
maintenance and standards of construction are likely to be poorer.

Alan

At 12:05 PM 9/7/2001 -0700, John C. Wheatley wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Tue Sep 11 09:05:50 2001



From: evgenia.pekarskaya-at-exxonmobil.com
Date: Tue, 11 Sep 2001 09:57:52 -0400
Subject: Re: ask help

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Yan,

Make sure that you withdraw the specimen from the polishing solution as
quickly as possible (!) and rinse immediately (!). I don't know what you
are using to rinse your specimens. If your electropolishing solution is
based on methanol, than it is a good idea to rinse it in methanol. I
normally rinse my specimens twice in two different beakers with methanol
or ethanol.
Good luck!

Evgenia

**********************************************************
Evgenia Pekarskaya
ExxonMobil Research & Engineering Co.
1545 Route 22 East, Rm. LB388
Annandale, NJ, 08801
Tel. (908) 730-2272
Fax (908) 730-3355




"yan.hu"
{yan.hu-at-brunel. To: microscopy-at-sparc5.microscopy.com
ac.uk} cc:
Subject: ask help

09/11/01 03:35
AM





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Dear Listers,

I got a problem when preparing TEM foils of Al-alloy by means of
electro-polishing. A dark film produced on the specimen surface when it
was been polishing. I think, the polishing condition is right and
listed below:

Solution, 25 % Nitric acid plus 75 % Mechenol
Temperature, about -30 degree C
Voltage, 15-20V

I would be pleased to hear from anyone who has an idea to sort out this
problem.


Regards

Yan
----------------------

yan.hu-at-brunel.ac.uk







From daemon Tue Sep 11 09:48:26 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 11 Sep 2001 09:46:46 -0500
Subject: Facility support survey redux

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Micro listers,

This is the second round. I have not received enough responses to
give any meaningful data. If you are interested in the results of
this survey, but have not responded for your facility, please do so.
Thanks!

This is a survey that was passed out at the Facility Managers Meeting
at this year's M&M meeting. We've only gotten 6 responses so far, and
many more are needed for valid results (although obviously only one
answer per facility). Please take a couple of minutes to fill this
form in, and email back to me. Thank you!

Phil

MICROSCOPY FACILITY SUPPORT

This survey will help compile the nature and amount of support
provided to microscopy facilities by universities, colleges, and
research institutes. We would like to determine how much facility
support is provided by the institution, and how much must be
generated by user fees or other sources, on a per cent basis.

If we get enough responses to have valid data, we will post the
results on the microscopy listserver and send them to the MSA
bulletin.
Names of institutions will not be included in the reported
results.

Institution:
Location:
Nature of institution (Academic, Commercial, private research, etc.)

Type of Facility (institution core, satellite, departmental, etc):

(Place an 'X' by the appropriate answer)
Predominately:
Biological
Materials
Both

User base:
Service
Multi-user
Both

Type of microscopes:
SEM
FESEM
ESEM or LV
TEM
FETEM
Major optical (Specify)
Scanning Probe (specify)
Other (specify)

Approximately what *per cent* of the funds for each of the following
is provided by
A) your institution, B) user fees, or C) grants

Salaries:
A)
B)
C)

Instrument Maintenance (Service contracts, instrument insurance, etc):
A)
B)
C)

Replacement of existing equipment:
A)
B)
C)

Purchase of new equipment (not replacement for but addition to
existing equipment):
A)
B)
C)

Supplies:
A)
B)
C)

Other operating expenses:
A)
B)
C)

Are there other microscope facilities at your institution? if
so, please indicate approximate number having electron
microscopes. Ideally we would like a form filled out for each
major facility (multiple instruments) at your institution if
there are more than one.

Please include additional information such as novel funding
ideas for your facility or for others at your institution.

Please email your responses to Philip Oshel
peoshel-at-facstaff.wisc.edu
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Tue Sep 11 10:21:12 2001



From: yan.hu :      yan.hu-at-brunel.ac.uk
Date: Tue, 11 Sep 2001 16:14:29 +0100 (BST)
Subject: electropolishing

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Many thanks to everyone who responded to my question about
electropolishing. The details of my experiment are:
1. it is an Al-Mg-Si alloy.
2. it is a twin jet-polish by Tenupole
3. The flow rate I used was 6.
4. The black film possibly appeares at the beginning of the polishing
not after the foil prepared.
5. I noted that the current is more than 500 mA this time, not about
100 mA as before. This is a difference. Is it due to the problem of the
instruments.

Yan Hu
----------------------

yan.hu-at-brunel.ac.uk



From daemon Tue Sep 11 14:01:53 2001



From: stacey andringa :      andrina-at-email.uc.edu
Date: Tue, 11 Sep 2001 14:52:06 -0400
Subject: mouse ears

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Has anyone done serial sections of decalcified mouse ears? I have been
given some that were decalcified in EDTA for about 10 days,
post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
grad student did the orienation for embedding, but along what plane I do
not know. Each orientation is a little different. Can anyone recommend
a consistent method of embedding?

Stacey.Andringa-at-uc.edu



From daemon Wed Sep 12 02:38:41 2001



From: R. Cross :      r.cross-at-ru.ac.za
Date: Wed, 12 Sep 2001 09:19:42 +0200
Subject: Black Tuesday

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Dear friends and colleagues

This message is not relevant to our normal topics of discussion but
I hope that under the circumstances you, Nestor, and others will
forgive me for this once-off breach of the rules.

I am sure I speak on behalf of all subscribers to the MSA listserver
when I express heartfelt shock and indignation at yesterdays
tragedies in New York City, Washington DC and near Pittsburg.
Those of us from elsewhere in the world assure our colleagues in
the US that we share your grief, and offer our support at this
difficult time. We extend our sincere condolences to those who
have lost family members, friends and colleagues and we wish a
speedy recovery to those who have been injured.

God bless, vasbyt and sterkte*.

Rob

* loosely translated = "hang in there and be strong"


=======================================================
Robin H Cross
Director : Electron Microscopy Unit, and
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm
http://www.icem15.com
===========================================================================
Remember that ICEM-15 takes place in Durban, South Africa in September 2002
===========================================================================


From daemon Wed Sep 12 04:29:32 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 12 Sep 2001 10:34:19 +0100
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

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I have watched this conversation with interest and must admit that I am
a very nervous about a student in a High School isolating pathogens on
blood agar at 37 deg C. This pre-supposes that there are trained
microbiologists, safe handling facilities and disposal methods for
unknown human pathogens.

If someone was to perform a risk assessment on this activity would they
even consider letting an unsupervised first year degree student isolate
unknown pathogens in the microbiology department?

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Jill Verlander Reed wrote:
}
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Katelyn,
} I will only add to Nelson Conti's remarks that you should be able to buy
} "blood-agar plates," which are Petri dishes already filled with a
} sterile, general bacterial culture medium, as well as sterile
} cotton-tipped swabs, from a medical supply store in your area. My
} daughter did a project along the same lines as yours and that's where we
} got the supplies. The dishes came in packs of 10, I think and they only
} cost a few dollars.
} We kept the inoculated dishes in a warm place. Around body temperature
} (37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
} If you find bacterial colonies that have a clear ring around them,
} instead of the red of the rest of the blood agar, that is an indication
} that the bacteria in that colony are pathogenic (disease-producing).
} Good luck, and have fun.
}
} Jill Verlander Reed, D.V.M.
} Associate Scientist
} Director, College of Medicine Electron Microscopy Core Facility
} University of Florida
} P.O. Box 100215
} Gainesville, FL 32610
} verlaj-at-medicine.ufl.edu
} Phone: (352) 846-0820
} Fax: (352) 846-3299

===============================================================
ORIGINAL MESSAGE:

Email: sbcook-at-bcpl.net
Name: Katelyn Cook

Organization: St. Michael the Archangel

Education: 6-8th Grade Middle School

Location: Baltimore, MD

Question: I am planning to do a science fair project about germs and
the importance of hand washing. I want to collect germs from common
public places such as doorknobs, handrails, telephones, sink faucets,
etc. What is the best way to collect the germs? Will I need to grow
the germs in a petri dish after I collect them? Will I be able to
identify different types of germs using my microscope?

---------------------------------------------------------------------------


From daemon Wed Sep 12 07:48:03 2001



From: =?iso-8859-1?q?Valeria=20L=20Burgos?= :      valaburg-at-yahoo.com.ar
Date: Wed, 12 Sep 2001 09:38:51 -0300 (ART)
Subject: fixatives - a quick question

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Hi everyone! I wonder if this is the right place for my doubt... I
need if someone could tell me whether a major and important
difference exists between Paraformaldehyde 4% vs Formaldehyde4%
as a fixative for brain tissue.
Thank you
:)
Valeria Burgos

_________________________________________________________
¿Lo probaste?
Correo gratis y para toda la vida en http://correo.yahoo.com.ar


From daemon Wed Sep 12 07:54:55 2001



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Wed, 12 Sep 2001 07:49:17 -0500
Subject: mouse ears

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I embed and section mouse cochleas on a regular basis (anywhere between 1
and 4 microns in thickness). Is your tissue dissected down to the
spiral-shaped bone of the cochlea itself? Do you want sections along the
plane of the modiolus (the center columnar structure of the cochlea) so that
you get a cross-sectional view of three to five organ of Corti profiles?
The cochleas I embed still have some vestibular structures remaining.

If so, I embed the cochleas in flat silicone molds with the flat surface of
bone (which faces the brain) facing the bottom of the mold and the spiral
surface up. After the resin has been polymerized and the blocks removed
from the mold, I remount the block onto flat-ended cylinder blanks. (These
are made by closing the cap of a BEEM capsule, cutting off the pyramidal
tip, placing them with the closed cap down, filling them to the rim with
resin, polymerizing and removing the BEEM capsule.) The block is remounted
(I usually use SuperGlue) onto the blank with the cochlea side down (the
curved surface now faces down and the flat bony surface faces up). Excess
resin is removed by trimming the block as usual. When I'm cutting into the
block, I continually check the orientation. I know I'm close the proper
orientation if I start to get profiles of the maculae and/or cristae in the
vestibular structures and a base-to-apex view of the modiolus. Continue to
adjust until you get the desired orientation.

I hope this gets you started.

Jaclynn M. Lett, Research Technician III jlett-at-cid.wustl.edu

Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030

-----Original Message-----
} From: stacey andringa [mailto:andrina-at-email.uc.edu]
Sent: Tuesday, September 11, 2001 1:52 PM
To: Microscopy-at-sparc5.microscopy.com


Has anyone done serial sections of decalcified mouse ears? I have been
given some that were decalcified in EDTA for about 10 days,
post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
grad student did the orienation for embedding, but along what plane I do
not know. Each orientation is a little different. Can anyone recommend
a consistent method of embedding?

Stacey.Andringa-at-uc.edu



From daemon Wed Sep 12 12:08:40 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Wed, 12 Sep 2001 11:59:08 -0500
Subject: LM - counting agglutinated blood cells

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Hi, all,

We have been working with hemagglutination tests lately (something
new for our EM lab), and are looking for a quick and easy way to
score fields captured using a standard LM with a digital camera.

We have tried some of the image analysis packages available, but have
not found them very easy to use. Also we have some questions about
the images themselves - how many blood cells have to be stuck
together before it's considered to be a clump? A clump is a 3
dimensional shape, so this has to be taken into account, doesn't it?

If anyone is doing this routinely and wouldn't mind giving us a few
hints (or references), we'd really appreciate it.

Thanks in advance for any help you can give.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca


From daemon Wed Sep 12 13:26:33 2001



From: Wang, Zhiyu :      Zhiyu_Wang-at-Maxtor.com
Date: Wed, 12 Sep 2001 13:18:26 -0500
Subject: RE: Spectral Imaging with ISIS

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Hi, all:

The term of "Spectral Imaging" is not well defined yet. If it means the
distribution of certain number of elements on the surface of sample, that is
the X-ray mapping. If it means all of possibility on the element table
(means energy spectrum) on the entire surface of sample (mapping), I do not
think the Oxford ISIS can do it. The simple way is to use the "Area
Analysis" in stead of point analysis. That will give you all element
signature on the selected area.

In ISIS 300, there is an EBSD package attached (need to get key, again),
this package can do the similar thing. It collects the electron backscatter
diffraction pattern (EBSP) on a single point and compares it with a signed
crystallographic pattern (one needs to be known and signed). It does this
on an array point by point and displays the matrix as a map. The final
result shows the contrast of crystallography on the surface layer ( {50nm).
This can be called as the imaging of diffraction property of given crystal.
It is a very good tool for the study of grain size and boundary.

It is up to user's interest, what is your goal for "spectral imaging". ISIS
has another software package, call Particle Analyzer (Gun shot analyzer).
Again, user needs to give information about the most possible present
elements, and the software can do the rest to see if these elements are
shown up. Without these pre-defined information, software will have no
direction to go and have no goal to be achieved.

Regards,

Zhiyu Wang
Maxtor Corp.



-----Original Message-----
From: Zhiyuw [mailto:zhiyuw-at-home.com]
Sent: Tuesday, September 11, 2001 9:51 PM
To: zhiyu_wang-at-maxtor.com
Subject: Fw: Spectral Imaging with ISIS


----- Original Message -----
From: "Warren E Straszheim" {wesaia-at-iastate.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 23, 2001 10:32 AM
Subject: RE: Spectral Imaging with ISIS


}
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}
}
} I am pretty sure that Alwyn is not talking about x-ray
mapping. If that
was
} all he wanted, I suppose the key disk is all that would be
needed if he
} already has the other imaging applications and hardware
installed.
}
} Someone suggested using Auto to scan an image for multiple
spectra.
Indeed,
} the software and hardware should allow it. The spectra
might then be
} exported and processed by some other package to perform
the type of
} component analysis that has been described at MSA over the
last few years.
} My current ISIS x-ray application (version 3.32) has the
option of storing
} the x-ray data in single column MSA format. Previous
versions placed
} multiple channels on a single line.
}
} One hitch would be the limitation on the number of spectra
per job. I
think
} there is a limit of 1000 or so built into the database
used for managing
} the data. I suppose it could be changed to another number,
but we bumped
} into it some time back.
}
} That would mean you could do a 32x32 raster of points
saving a spectrum at
} each. A four second acquisition per point would mean a bit
more than an
} hour for acquisition. That would not be too bad, but the
spatial
resolution
} would probably be too low to be very useful. But the data
should readily
} fit onto hard drives with capacities of a few gigabytes.
}
} I would be interested in seeing how the data processing is
handled. If
} someone makes it work, I would be very interested in
hearing the details.
}
} Warren
}
} At 10:33 AM 8/23/2001 -0400, you wrote:
}
} -----------------------------------------------------------------------.
} } This is correct, the keydisk is all that is required for
} } the software to be enabled. However, there may be
hardware
} } required, too, at least a cable interface (RS232?) to the
} } microscope. I believe the ISIS Autobeam likes to select
} } its own scan rates. You may need to speak with Oxford
re:
} } compatibility with your particular microscope.
} }
} } Matt
} }
} } Matthew J. Lynn
} } Center for Advanced Microscopy
} } University of Miami
} } (305)284-4736
} } mlynn-at-miami.edu
} }
} }
} } On Wednesday, August 22, 2001 9:23 PM, Fred Pearson
} } [SMTP:eoptics-at-mcmail.cis.mcmaster.ca] wrote:
} } }
} } } Alwyn:
} } }
} } } By spectral images, do you mean xray mapping? A "key"
disk for the
ISIS
} } } software program is required to activate the xray
mapping facility.
Of,
} } } course this will cost a bit of money to purchase from
Oxford. When
the
} } } ISIS program is loaded initially, all the software
required to use the
} } } program is there, but activating the unpaid for parts
of the program
is
} } } the thing.
} } }
} } } Fred
} } }
} } } On Wed, 22 Aug 2001, Alwyn Eades wrote:
} } } } We have an Oxford ISIS EDS system. As it stands it
is not set up to
} } } } acquire spectral images. We would like to be able
to do spectral
image
} } } } acquisition on this machine. Is there anyone out
there who knows
how
} } } } the system may be modified (macros, interfaces,
whatever) to do it?
We
} } } } do have Digital Micrograph (on a Mac whereas ISIS is
on a PC), if
that
} } } } helps.
} } } } ..........
} } } } Alwyn Eades
} } } } Department of Materials Science and Engineering
} } } } Lehigh University
} } } } 5 East Packer Avenue
} } } } Bethlehem
} } } } Pennsylvania 18015-3195
} } } } Phone 610 758 4231
} } } } Fax 610 758 4244
} } } } jae5-at-lehigh.edu
}
}
}


From daemon Wed Sep 12 13:45:41 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Wed, 12 Sep 2001 14:38:22 -0400
Subject: Postdoctoral Position Available immediately

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Hi, all,

I am posting this for a colleague.

Paula

___________________________________________________________

Postdoctoral Position Available

Wageningen University
Dept Agrotechnology and Food Sciences

Postdoctoral Research Position in Cell Wall Microscopy

A vacancy for a Postdoctoral Position is available in the area of microscopy and functionality of cell walls as part of the research program of Dr. Henk Schols, Laboratory of Food Chemistry. The successful candidate will be studying serum separation in tomato products and the influence of processing at the cell wall level. Ideally immunolocalization techniques will be used to localise specific polysaccharides in these samples.

Candidates must originate from outside The Netherlands and hold a recent Ph.D. in a related field. The appointment is for 9 months and is available immediately.

For more information, interested candidates should contact:


Dr. Henk Schols
Wageningen University
Dept Agrotechnology and Food Sciences
Laboratory of Food Chemistry
Bomenweg 2
6703 HD Wageningen
The Netherlands

tel 31 317 482239
fax 31 317 484893

E-mail: Henk.Schols-at-chem.fdsci.wag-ur.nl
http://www.ftns.wau.nl/lmt/lmc/


¯---------------------------------------------------------------------------------------------------------------------

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Wed Sep 12 14:43:57 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Wed, 12 Sep 2001 15:35:10 -0400
Subject: Re: Black Tuesday

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Thank You Randy.

Here is Washington, DC things are starting to get back to normal. Though what is normal has now changed. Our city was shut down for one day by cowardly terrorist acts. We are back at work and are carrying on. We will not let this break our spirit. The "Sleeping Giant" has been aroused and it is much larger than the perpetrators of this act realized as the Giant includes the global community.

Courage,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

} } } "R. Cross" {r.cross-at-ru.ac.za} 09/12/01 03:19AM } } }
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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear friends and colleagues

This message is not relevant to our normal topics of discussion but
I hope that under the circumstances you, Nestor, and others will
forgive me for this once-off breach of the rules.

I am sure I speak on behalf of all subscribers to the MSA listserver
when I express heartfelt shock and indignation at yesterdays
tragedies in New York City, Washington DC and near Pittsburg.
Those of us from elsewhere in the world assure our colleagues in
the US that we share your grief, and offer our support at this
difficult time. We extend our sincere condolences to those who
have lost family members, friends and colleagues and we wish a
speedy recovery to those who have been injured.

God bless, vasbyt and sterkte*.

Rob

* loosely translated = "hang in there and be strong"


=======================================================
Robin H Cross
Director : Electron Microscopy Unit, and
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
Rhodes University, PO Box 94, Grahamstown, South Africa
tel: +27 46 603 8168/9, fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm
http://www.icem15.com
===========================================================================
Remember that ICEM-15 takes place in Durban, South Africa in September 2002
===========================================================================




From daemon Wed Sep 12 14:57:08 2001



From: Dmrelion-at-aol.com
Date: Wed, 12 Sep 2001 15:52:09 EDT
Subject: Wild M5A microscope

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Anyone know if there was ever a trinocular head available for a Wild M5A
binocular microscope and, if so, where to get a used one?

Thanks,

Don

Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Wed Sep 12 15:07:56 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 12 Sep 2001 16:05:57 -0400
Subject: BLACK TUESDAY

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Dear Listers:

I work on the University side of a large Medical center and yesterday after
the attack in New York and Washington we received a broadcast e mail from
the administration asking for blood. A couple of hours later I got to the
blood bank and found a huge mob of medical workers in line to donate blood.
Today the line was more then three hours long. I am going to wait a couple
of days before donating blood. I think its realalistic that the demand for
blood will be very high for at least a few weeks to come. I urge all
healthy adults to join me in donating blood as its the only real thing that
private citizens can do to help in this unprecedented catastrophe.
Sincerely, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Wed Sep 12 15:45:48 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 12 Sep 2001 15:39:05 -0500
Subject: Re: mouse ears

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Hi Stacey,

There are a couple of ways to reduce the orientation variation. One way
is to use flat-bottomed Beem capsules for embedding molds (I haven't got
the order info on these with me, but I can get it to you later, or you
may get a reply from someone who has the info handy). These molds have
a small enough surface area at the bottom that you can place the bulla
or
inner ear in them, say medial side down and they don't rock or float
out of position. (Fill the mold before manipulating the specimen.)

Another way I have for embedding the cochlea is to split it in half,
down the modiolus just prior to embedding and place the cut surface
face down in the molds. I use half of a double edged razor blade to
split it under a dissecting scope and I orient the cut from the apex
to the base with the round window membrane perpendicular to the top
surface. If you process the cochlea to this point before you split it,
it is much firmer and tougher to work with and you won't lose as much
tissue, but you do lose some. Hope this helps.

Karen Pawlowski, Ph.D.

stacey andringa wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Has anyone done serial sections of decalcified mouse ears? I have been
} given some that were decalcified in EDTA for about 10 days,
} post-osmicated, dehydrated, infiltrated and embedded in Spurr resin. A
} grad student did the orienation for embedding, but along what plane I do
} not know. Each orientation is a little different. Can anyone recommend
} a consistent method of embedding?
}
} Stacey.Andringa-at-uc.edu


From daemon Wed Sep 12 16:00:50 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 12 Sep 2001 15:56:02 -0500
Subject: Re: Ask-A-Microscopist:Germs and Science Projects

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Malcolm,

I find your comment interesting as I have volunteered to judge several
Regional and National Science Fairs for high school aged students and
have seen this particular experiment carried out by a number of the
students. The students that I choose to judge are actually the younger
group, who are "middle school" aged and I doubt the majority of them
are getting much outside help, other than their science teachers or
they would have picked a topic that was more unique (or geared to what
the expert was doing).

I'm sure the petri dishes never leave the science lab. and the students
were gloves while handling the cultures, but that is probably the
extent of it....

Karen Pawlowski, Ph.D.


Malcolm Haswell wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} I have watched this conversation with interest and must admit that I am
} a very nervous about a student in a High School isolating pathogens on
} blood agar at 37 deg C. This pre-supposes that there are trained
} microbiologists, safe handling facilities and disposal methods for
} unknown human pathogens.
}
} If someone was to perform a risk assessment on this activity would they
} even consider letting an unsupervised first year degree student isolate
} unknown pathogens in the microbiology department?
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Jill Verlander Reed wrote:
} }
} } ------------------------------------------------------------------------
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} } -----------------------------------------------------------------------.
} }
} } Dear Katelyn,
} } I will only add to Nelson Conti's remarks that you should be able to buy
} } "blood-agar plates," which are Petri dishes already filled with a
} } sterile, general bacterial culture medium, as well as sterile
} } cotton-tipped swabs, from a medical supply store in your area. My
} } daughter did a project along the same lines as yours and that's where we
} } got the supplies. The dishes came in packs of 10, I think and they only
} } cost a few dollars.
} } We kept the inoculated dishes in a warm place. Around body temperature
} } (37 degrees C, 98-99 degrees F) is ideal is you can find such a place.
} } If you find bacterial colonies that have a clear ring around them,
} } instead of the red of the rest of the blood agar, that is an indication
} } that the bacteria in that colony are pathogenic (disease-producing).
} } Good luck, and have fun.
} }
} } Jill Verlander Reed, D.V.M.
} } Associate Scientist
} } Director, College of Medicine Electron Microscopy Core Facility
} } University of Florida
} } P.O. Box 100215
} } Gainesville, FL 32610
} } verlaj-at-medicine.ufl.edu
} } Phone: (352) 846-0820
} } Fax: (352) 846-3299
}
} ===============================================================
} ORIGINAL MESSAGE:
}
} Email: sbcook-at-bcpl.net
} Name: Katelyn Cook
}
} Organization: St. Michael the Archangel
}
} Education: 6-8th Grade Middle School
}
} Location: Baltimore, MD
}
} Question: I am planning to do a science fair project about germs and
} the importance of hand washing. I want to collect germs from common
} public places such as doorknobs, handrails, telephones, sink faucets,
} etc. What is the best way to collect the germs? Will I need to grow
} the germs in a petri dish after I collect them? Will I be able to
} identify different types of germs using my microscope?
}
} ---------------------------------------------------------------------------


From daemon Wed Sep 12 20:22:17 2001



From: Gerry Nash :      gerry.nash-at-antdiv.gov.au
Date: Thu, 13 Sep 2001 11:13:51 +1000
Subject: For the USA

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To all our Amercian fellow microscopists and friends,
The Australian Society for Electron Microscopy Inc sends its deepest
heartfelt condolences to you all and your loved ones at this time of
such tragic and unbelievable loss.
Our warmest thoughts and prayers are with you all.
Gerry Nash
President ASEM Inc
--
Geraldine (Gerry) Nash
Electron Microscopist

EM Unit
Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351

Visit our EM Unit web site:
http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
and
Australian Antarctic Division web site: http://www.aad.gov.au/


From daemon Wed Sep 12 20:25:21 2001



From: Gerry Nash :      gerry.nash-at-antdiv.gov.au
Date: Thu, 13 Sep 2001 11:19:25 +1000
Subject: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all our Amercian fellow microscopists and friends,
The Australian Society for Electron Microscopy Inc sends its deepest
heartfelt condolences to you all and your loved ones at this time of
such tragic and unbelievable loss.
Our warmest thoughts and prayers are with you all.
Gerry Nash
President ASEM Inc
--
Geraldine (Gerry) Nash
Electron Microscopist

EM Unit
Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351

Visit our EM Unit web site:
http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
and
Australian Antarctic Division web site: http://www.aad.gov.au/


From daemon Thu Sep 13 07:22:29 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Thu, 13 Sep 2001 08:07:53 -0400 (EDT)
Subject: Re: For the USA

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Thank you, Gerry.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Thu, 13 Sep 2001, Gerry Nash wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all our Amercian fellow microscopists and friends,
} The Australian Society for Electron Microscopy Inc sends its deepest
} heartfelt condolences to you all and your loved ones at this time of
} such tragic and unbelievable loss.
} Our warmest thoughts and prayers are with you all.
} Gerry Nash
} President ASEM Inc
} --
} Geraldine (Gerry) Nash
} Electron Microscopist
}
} EM Unit
} Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
} Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351
}
} Visit our EM Unit web site:
} http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
} and
} Australian Antarctic Division web site: http://www.aad.gov.au/
}
}



From daemon Thu Sep 13 07:47:10 2001



From: Alexander Black :      alexander.black-at-nuigalway.ie
Date: Thu, 13 Sep 2001 13:31:51 +0100
Subject: Heartfelt sympathy

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To all our American colleagues:
On behalf of the Microscopical Society of Ireland, I would like to
extend
deepest sympathy and condolences to those who have been affected
directly or
indirectly by the atrocious terrorist attacks on the American Nation.

Sincerely,
Alexander Black,
President,
Microscopical Society of Ireland



Department of Anatomy
National University of Ireland, Galway.





From daemon Thu Sep 13 09:26:10 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 13 Sep 2001 10:16:02 -0400
Subject: RE: fixatives - a quick question

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Morning Valeria,

There is a great difference. Paraformaldehyde is a POLYMER of
formaldehyde(HCHO). Paraformaldehyde will yield HCHO if you heat the dry
powder to around 90 degrees C (NOT RECOMMENDED!!!). I am sending a document
under separate cover that will explain, but briefly, there are three words
that are important here. Formalin (commercial, 40% HCHO solution in HOH,
that contains from 5-15% methanol to prevent the HCHO from polymerizing),
Paraformaldehyde (the polymer, a white powder), and formaldehyde solutions
(generated from paraformaldehyde suspensions in water or buffer, which I
designate as "pHCHO"). The attachment to the following email will explain
in greater detail.
Good question!!!

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Thu Sep 13 11:40:28 2001



From: tea meulia :      meulia.1-at-osu.edu
Date: Thu, 13 Sep 2001 12:39:35 -0500
Subject: anti-dsDNA antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for antibodies against double-stranded DNA, and eventually
antibodies against histones. The two companies that I contacted, Biogenex
and Pierce, discontinued its productions. Does anybody know for a supplier?

Tea


***************************************
Tea Meulia
Research Scientist and Director
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************




From daemon Thu Sep 13 17:39:01 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Thu, 13 Sep 2001 15:24:01 -0700
Subject: Announcement correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We have had a last minute change in the program for our program for
Microscopy and Digital Imaging on Sept. 27, 2001

1:15 "Imaging Tissue Morphogenesis in Zebrafish Embryos."
Mark Cooper, University of Washington

Regards,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************

C:} The box said "Requires Windows95 or better". So I bought a
Macintosh.
**************************************************************************





From daemon Thu Sep 13 21:32:38 2001



From: Edward_Principe-at-amat.com
Date: Thu, 13 Sep 2001 19:20:38 -0700
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In regard to what is "appropriate", I share my own view.

I agree that science and this list server under these circumstances should
be divorced from any shade of political statement.

The science community (emphasis on the community) is not divorced from
emotion, thank goodness.

I believe we graciously accept the simple gestures of sympathy and not
imply insult or rejection.

Justification: It is a historical, if not still surreal and forever
infamous, time for world civilization.

With best regards and appreciation,
Ed


Angela Klaus {avklaus-at-amnh.org} on 09/13/2001 05:07:53 AM


To: Gerry Nash {gerry.nash-at-antdiv.gov.au}
cc: microscopy-at-sparc5.microscopy.com


Thank you, Gerry.

Best regards,

Angela

Angela V. Klaus

Director, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977


On Thu, 13 Sep 2001, Gerry Nash wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all our Amercian fellow microscopists and friends,
} The Australian Society for Electron Microscopy Inc sends its deepest
} heartfelt condolences to you all and your loved ones at this time of
} such tragic and unbelievable loss.
} Our warmest thoughts and prayers are with you all.
} Gerry Nash
} President ASEM Inc
} --
} Geraldine (Gerry) Nash
} Electron Microscopist
}
} EM Unit
} Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050
} Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323
351
}
} Visit our EM Unit web site:
} http://www.aad.gov.au/science/ResearchResources/em_unit/default.asp
} and
} Australian Antarctic Division web site: http://www.aad.gov.au/
}
}






From daemon Fri Sep 14 09:30:58 2001



From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: 14 Sep 2001 10:11:51 -0400
Subject: Wehnalt cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
About a year ago there was a discussion about using ammonia to clean
Wehnelts. I dutifully saved all the emails because the technique looked
interesting. Of course, when I went looking for these emails I couldn't
find them. I looked on the list archives but was not able to come up
with anything. Could someone who uses this technique contact me off
list?

Thanks
--
Glenn

===============================================================================
Glenn Poirier 3450 University St, rm. 238
MicroAnalytical Laboratory Montreal, Qc
Earth and Planetary Sciences tel (514) 398 6774
McGill University fax (514) 398 4680
email: glennp-at-eps.mcgill.ca http://castaing.eps.mcgill.ca

++ Millenium hand and shrimp ++
===============================================================================



From daemon Fri Sep 14 09:48:17 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 14 Sep 2001 10:38:30 -0400
Subject: Re: For the USA

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for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 14 Sep 2001 10:30:23 -0400
Importance: Normal


Thank you, all, for your support. I would like to share an article I
received
with everyone, especially my fellow Americans. I hope nobody is offended
by it, for I do not intend, or wish, for that to happen.

Darrell

} I know you are all receiving a lot of emails like this today...but read
} on:
}
} Subject: This, from a Canadian newspaper, is worth sharing.
}
} America: The Good Neighbor.
}
} Widespread but only partial news coverage was given recently to
} remarkable editorial broadcast from Toronto by Gordon Sinclair,
} a Canadian television commentator.
} What follows is the full text of his trenchant remarks as
} printed in the Congressional Record:
}
} "This Canadian thinks it is time to speak up for the Americans as
} the most generous and possibly the least appreciated people
} on all the earth.
} Germany, Japan and, to a lesser extent, Britain and Italy were
} lifted out of the debris of war by the Americans who poured in
} billions of dollars and forgave other billions in debts.
} None of these countries is today paying even the interest
} on its remaining debts to the United States.
}
} When France was in danger of collapsing in 1956, it was the
} Americans who propped it up, and their reward was to be insulted
} and swindled on the streets of Paris. I was there. I saw it.
}
} When earthquakes hit distant cities, it is the United States
} that hurries in to help. This spring, 59 American communities
} were flattened by tornadoes. Nobody helped.
}
} The Marshall Plan and the Truman Policy pumped billions of dollars
} into discouraged countries. Now newspapers in those countries are
} writing about the decadent, warmongering Americans.
}
} I'd like to see just one of those countries that is gloating over
} the erosion of the United States dollar build its own airplane.
} Does any other country in the world have a plane to equal
} the Boeing Jumbo Jet, the Lockheed Tri-Star, or the Douglas DC10?
} If so, why don't they fly them?
} Why do all the International lines except Russia fly American
} Planes?
}
} Why does no other land on earth even consider putting a man or
} woman on the moon?
} You talk about Japanese technology, and you get radios.
} You talk about German technology, and you get automobiles.
}
} You talk about American technocracy, and you find men on the
} moon-not once, but several times-and safely home again.
} You talk about scandals, and the Americans put theirs right
} in the store window for everybody to look at.
}
} Even their draft-dodgers are not pursued and hounded. They are
} here on our streets, and most of them, unless they are breaking
} Canadian laws, are getting American dollars from ma and pa at home
} to spend here.
}
} When the railways of France, Germany and India were breaking down
} through age, it was the Americans who rebuilt them.
} When the Pennsylvania Railroad and the New York Central went broke,
} nobody loaned them an old caboose.
} Both are still broke.
}
} I can name you 5000 times when the Americans raced to the help of
} other people in trouble. Can you name me even one time when
} someone else raced to the Americans in trouble?
} I don't think there was outside help even during the San Francisco
} earthquake.
}
} Our neighbors have faced it alone, and I'm one Canadian who is
} damned tired of hearing them get kicked around.
} They will come out of this thing with their flag high.
} And when they do, they are entitled to thumb their nose at
} the lands that are gloating over their present troubles. I hope
} Canada is not one of those."
}
} Stand proud, America!
}
} I would hope that each of you would send this to as many people
} as you can and emphasize that they should send it to as many
} of their friends until this letter is sent to every person on the web.



From daemon Fri Sep 14 10:17:02 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 14 Sep 2001 11:16:01 -0400
Subject: RE: Cleaning WWehnelt cylinders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Various methods for cleaning Wehnelt cylinders, and other internal
parts of vacuum systems, are discussed in some detail in Section
2.10.4c (see bottom of page 72) of the book Vacuum Methods in
Electron Microscopy (for info about this book see:
http://www.2spi.com/catalog/books/book48.html and
http://pup.princeton.edu/titles/6484.html).

Disclaimer: I make about $1.50 from each copy of this book sold.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Fri Sep 14 10:44:18 2001



From: steven wintonick :      crimsem-at-hotmail.com
Date: Fri, 14 Sep 2001 11:34:24 -0400
Subject: Thanks from NYC and USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all those people who have expressed their concerns,

I would like to thank all of those people who have sent their heartfelt
messages and words of encouragement. Especially now in our time of need, it
is uplifting to know all of our fellow microscopists from other nations are
expressing their concern and support. During this time, when it is difficult
to sort out our emotions, all of your words have helped to restore some of
my faith in humanity.

Thanks you.
Steve

_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp



From daemon Fri Sep 14 12:10:57 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 14 Sep 2001 12:59:31 -0400
Subject: Please accept my apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I must humbly apologize for the "article" I sent. A coworker
has informed me that it is a hoax. My first instinct was to not
send it. I made the mistake of trusting the source, and allowing
the video images of crowds of people cheering the deaths of
tens of thousands of Americans in the matter of a few hours,
cloud my better judgment.

I was, and am, sincere in my thanks for the support expressed
on the list, and from all other sources.

Darrell

(These are my opinions. You can blame no one else for them.)



From daemon Fri Sep 14 12:19:17 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com (by way of Nestor J.
Date: Fri, 14 Sep 2001 11:53:14 -0500
Subject: Have VP-SEM with tensile stage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

Usually we do our own SEM work, but a special job requirement may arise
which I cannot accommodate using our equipment. It may be desirable to
tensile fracture a thin ceramic sheet (predominately zirconia) and examine
the fracture surface without breaking vacuum. EDS would likely be required
as well. Magnification requirements *should* range from about 20x to 5000x.
It would not surprise me, however, if 30-50 Kx might provide useful
information.

If you have the equipment and take "outside" work, please contact me by
direct email or phone.

Direct line to my office and lab: 434.522.6111 ...If A/C 434 fails, try
the old one: 804

Thanks,

Woody White
McDermott Technology, Inc.
Lynchburg Research Center
Lynchburg, VA


McDermott site: http://www.mtiresearch.com/
Personal site: http://woody.white.home.att.net


From daemon Fri Sep 14 15:04:50 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 14 Sep 2001 12:54:51 -0700
Subject: Re: Wehnalt cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Glenn

I am not sure is it original recipe or it's my modification, but anyway,
the following works for me. I am sonicate Wehnelt cup in 1:1 diluted
ammonia solution (stock ammonia solution I believe is 30%) for 10-15 min,
rinse with deionized water and dry at +60 in the oven.

Good luck. Sergey



At 10:11 AM 9/14/01 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Fri Sep 14 17:40:54 2001



From: Kristof Kovacs :      kris-at-almos.vein.hu
Date: Fri, 14 Sep 2001 09:09:09 +0200
Subject: Sorrow and sympathy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wehnelt caps are usually made from stainless steel (304).

For JEOL wehnelts, I have had customers use "ammonium hydroxide hydrogen
peroxide 30% by volume". I am not a chemist so I can't give you the
particulars but this works great as it attacks everything except the
stainless steel. I usually let the cap soak in this solution overnight.


Regards,

Earl weltmer
----- Original Message -----
} From: "Glenn Poirier" {glennp-at-eps.mcgill.ca}
To: "Microscopy Listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 14, 2001 7:11 AM


Dear American Fellow Microscopists,
After the first moments of shock and grief let us express our deepest
sorrow and sympathy to the American microscopists in particular and to the
American people in general on behalf of all Hungarian microscopists. We
have so many friends and colleagues there...
To overcome these hard days and weeks let us wish you all strength both
physically and in your soul, work and fight together for a better world!
Kristof Kovacs

-----------------------------------------------------------
Dr. Kristof Kovacs
President, Hungarian Society for Microscopy
University of Veszprem
Veszprem, P.O.Box 158
H-8201 HUNGARY
Phone: +36-(88)-421-684, +36-(30)-930-3931
Fax: +36-(88)-423-091



From daemon Sun Sep 16 09:49:37 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Sun, 16 Sep 2001 10:29:20 -0400
Subject: Food Structure and Functionality Symposium 2002 - First

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Food Structure & Functionality Symposium 2002
May 5 - 8, 2002, Palais des Congrès de Montréal o Montréal, Québec, Canada.

An international symposium leading Food Structure & Functionality studies through the 21st century
"webaddress at the AOCS site (http://www.aocs.org/member/division/fsff/index.htm)"

Being held in conjunction with the 93nd AOCS Annual Meeting & Expo (www.aocs.org)

The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting.

Tentative Program Schedule
Sunday, May 5th
Short Course - Understanding structure-function relationships in food systems through specific localisation methods and microscopy. Contact: Marcel Paques (Paques-at-nizo.nl)

Monday, May 6th
Morning

Opening of symposium
Plenary Speaker
Dairy Applications Session.
Chairs: Mark Auty (mauty-at-moorepark.teagasc.ie) and Harjinder Singh (H.Singh-at-massey.ac.nz)
Lunch break
Afternoon
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl) and David Pechak (Dpechak-at-kraft.com)

Dedicated Poster Session
Division Board Meeting

Tuesday, May 7th
Morning
Agricultural Applications of Microscopy and Imaging Session./ joint with Feed Microscopy Division. Topic/Tentative title (will probably be revised): New Microscopic Techniques for Identifying Food/Feed Constituents and Contaminants
contacts: Mark Auty (mauty-at-moorepark.teagasc.ie) and Kim Koch

Lunch break: Division Luncheon and round table (expert) discussion. Topic TBA
Afternoon
Microbiology and Food Session.
Chairs Judy Arnold (jarnold-at-saa.ars.usda.gov) and Ida Yates (iyates-at-ars.usda.gov)

Food Structure and Functionality Forum - Division Members Meeting

Wednesday, May 8th
Morning
Ingredients and Food Processing Session.
Chairs: Diana Kittleson (dkittleson-at-pillsbury.com) and Bernhard Tauscher (bernhard.tauscher-at-bfe.uni-karlsruhe.de)

Lunch break
Afternoon
New Methods and Techniques for Food Structure and Functionality Analysis Session.
Chairs: Kathy Groves Kgroves-at-LFRA.co.uk and Maud Langton maud.langton-at-sik.se

Closure of Symposium.

*--------------------------------------------------------------------------------------------------------------------
For further information, please go to the websites indicated, contact session chairs listed above, or myself.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Sun Sep 16 19:03:32 2001



From: Cathy Gillespie :      cathy.gillespie-at-anu.edu.au
Date: Mon, 17 Sep 2001 09:55:36 +1000
Subject: TEM of wool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Can anyone recommend a method for preparing wool from sheep for TEM? Is it
easy to section?

Thanks

Cathy Gillespie



From daemon Sun Sep 16 19:06:08 2001



From: Peter Jordan :      emsi-at-pe.net
Date: Sun, 16 Sep 2001 16:57:12 -0700
Subject: Re: LETTER TO A TERRORIST...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Well, you hit the World Trade Center, but you missed America. You hit the
Pentagon, but you missed America. You used helpless American bodies, to take
out other American bodies, but like a poor marksman, you STILL
missed America.

Why? Because of something you guys will never understand. America isn't
about a building or two, not about financial centers, not about military
centers, America isn't about a place, America isn't even about a bunch of
bodies. America is about an IDEA. An idea, that you can go someplace where
you can earn as much as you can figure out how to, live for the most part
like you envisioned living, and pursue Happiness. (No guarantees that you'll
reach it, but you can sure try!)

Go ahead and whine your terrorist whine, and chant your terrorist litany:
"If you cannot see my point, then feel my pain." This concept is alien to
Americans. We live in a country where we don't have to see your point. But
you're free to have one. We don't have to listen to your speech. But you're
free to say one. Don't know where you got the strange idea that everyone has
to agree with you. We don't agree with each other in this country, almost as
a matter of pride.

We're a collection of guys that don't agree, called States. We united our
individual states to protect ourselves from tyranny in the world. Another
idea, we made up on the spot. You CAN make it up as you go, when it's your
country. If you're free enough. Yeah, we're fat, sloppy, easygoing goofs
most of the time. That's an unfortunate image to project to the world, but
it comes of feeling free and easy about the world you live in. It's
unfortunate too, because people start to forget that when you attack
Americans, they tend to fight like a
cornered badger. The first we knew of the War of 1812 was when England
burned Washington, DC, to the ground. Didn't turn out like England thought
it was going to, and it's not going to turn out like you think, either.

Sorry, but you're not the first bully on our shores, just the most recent.
No Marquis of Queensbury rules for Americans, either. We were the FIRST
and, so far, only country in the world to use nuclear weapons in anger.
Horrific
idea, nowadays? News for you bucko, it was back then, too, but we used it
anyway. Only had two of them in the whole world and we used 'em both.

Grandpa Jones worked on the Manhattan Project. Told me once that right up
until they threw the switch, the physicists were still arguing over whether
the Uranium alone would fission, or whether it would start a fissioning
chain reaction that would eat everything. But they threw the switch anyway,
because we had a War to win. Does that tell you something about American
Resolve?
}
So who just declared War on us? It would be nice to point to some real
estate, like the good old days. Unfortunately, we're probably at war with
random camps, in far-flung places. Who think they're safe. Just like the
Barbary Pirates did. Better start sleeping with one eye open. There's a
spirit that tends to take over people who come to this country, looking for
opportunity, looking for liberty, looking for freedom. Even if they misuse
it. The Marielistas that Castro emptied out of his prisons, were overjoyed
to find out how much freedom there was. First thing they did when they hit
our shores, was run out and buy guns. The ones that didn't end up dead,
ended up in prisons. It was a big PITA then (especially in south Florida),
but you're only the newest PITA, not the first.

You guys seem to be incapable of understanding that we don't live in
America, America lives in US! American Spirit is what it's called. And
killing a few thousand of us, or a few million of us, won't change it. Most
of the time, it's a pretty happy-go-lucky kind of Spirit. Until we're
crossed in a cowardly manner, then it becomes an entirely different kind of
Spirit.

Wait until you see what we do with that Spirit, this time. Sleep tight, if
you can. We're coming. And when we arrive, well, let's just say it won't
be pretty.




From daemon Sun Sep 16 20:13:03 2001



From: Gao Yihua :      GAO.Yihua-at-nims.go.jp
Date: Tue, 17 Sep 2013 10:06:04 +0900
Subject: thermochemical data of Ge3N4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleages,

Anyone knows which book or paper contains the thermochemical data of
Ge3N4 material.

Thank you very much in advance.


Yours sincerely

Gao Yihua


From daemon Mon Sep 17 05:46:46 2001



From: Martin Saunders :      martin-at-cmm.uwa.edu.au
Date: Mon, 17 Sep 2001 18:40:16 +0800
Subject: TEM sample prep: Ti extraction replica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I have been given a piece of titanium that contains small
precipitates of unknown composition and asked to prepare a TEM sample
so that the precipitates can be analysed. As it is only the
precipitates that are of interest I thought that the best approach
would be to make an extraction replica. However, to do that need to
find a way to etch away the titanium matrix while leaving behind the
unknown precipitates.

I would be grateful for any recommendations for a suitable etchant
that will preferentially remove the titanium matrix so that the
extraction replica can be made.

Regards,

Martin Saunders.
--

*****************************************

Dr. Martin Saunders,
Lecturer,
Centre for Microscopy and Microanalysis,
University of Western Australia,
Crawley,
Western Australia 6009,
Australia.

Phone: +61 8 9380 8092
Fax: +61 8 9380 1087
E-mail: martin-at-cmm.uwa.edu.au

*****************************************


From daemon Mon Sep 17 07:43:58 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Mon, 17 Sep 2001 08:35:23 -0400
Subject: Responsible reactions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues and Friends

It has been very positive to see, on the microscopy listserver, messages
from microscopy communities in other nations addressed to those of us
who live and work in the United States offering sympathy for the tragic
events of last Tuesday. These messages have, without exception, been
measured, responsible and generous. We are grateful for them.

Speaking for myself, I want to say how offended I was to read, on the
microscopy listserver, "Letter to a Terrorist" from Peter Jordan.
There are no terrorists on this list server. This is not an appropriate
place for this kind of polemic. I hope that those who take exception
to Mr. Jordan's remarks will refrain from responding to the substance of
what he says. The last thing we want is for the listserver to be taken
over by a political debate on the issues raised by the attacks on New
York and Washington.

Alwyn
--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Mon Sep 17 07:44:02 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Mon, 17 Sep 2001 22:37:30 +1000
Subject: to solute fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues

I like to dissolve fat in insects bodies what could be the best chemical
. benzine, carbon tetrachloride, or???

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Mon Sep 17 08:00:58 2001



From: Haeseong Lee :      haeseong-at-hanyang.ac.kr
Date: Mon, 17 Sep 2001 07:55:50 -0500
Subject: SEM imaging on CNT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


??
Hello.

Could anybody recommend the good method of sample preparation for SEM
imaging on carbon nano tubes?
The sample is a powder type including single wall carbon nanotubes (SWCNTs).
Thank you very much in advance.

Haeseong Lee

{mailto:haeseong-at-hanyang.ac.kr} haeseong-at-hanyang.ac.kr


From daemon Mon Sep 17 09:10:52 2001



From: efosten-at-mmm.com
Date: Mon, 17 Sep 2001 09:02:44 -0500
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



""} Subject: This, from a Canadian newspaper, is worth sharing.
}
} America: The Good Neighbor.
}
} Widespread but only partial news coverage was given recently to
} remarkable editorial broadcast from Toronto by Gordon Sinclair,
} a Canadian television commentator.
} What follows is the full text of his trenchant remarks as
} printed in the Congressional Record:""

Gordon Sinclair's piece was first broadcast in 1973 - see:

http://www.tysknews.com/Depts/Our_Culture/americans.htm


But it still applies.

Ev Osten

efosten-at-mmm.com
651-736-0104
fax: 651-733-0648

3M Company
Corporate Analytical Technology Center
3M Center, 201-BE-16
St. Paul, MN 55144-1000
U.S.A.



From daemon Mon Sep 17 09:54:00 2001



From: Ubirajara Pereira Rodrigues Filho :      uprf-at-iqsc.sc.usp.br
Date: Mon, 17 Sep 2001 11:42:57 -0300
Subject: thanks to answers on SEM of tempers in potteries ( specially shells )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear collegues;

I'd like to thanks to all who has collaborate for the answers on tempers
studies on potteries by SEM. I hope in brief get in touch with some of them.

I was trying to don't say a word on the terrorism subject, that because
sometimes for me when something so horrible happens with me I prefer stay
quiet on my side in order to recover my balance to overcome such pain.
However, I would like to expreess my deep feelings to American people and
gouvernement. Since the attacks I could not stop to think how terrible the
human been can be, even if sometimes the streets can show us terrible facts.
I've watched several chanels, brazilian and foreigners, and still can
understand what hapens and why that people has died. I would like to remark
my sympathy to your gouvernement which, in my oppinion, is doing their job
in this difficult situation as well as possible. We hope you can find find a
pacific way to solve this problem, however, if you can't don't mistake
several other countries around the world, including Brazil, and I belive
some arabian countries too, is supporting you and almost ready to stay
beside you. You know the American spirit is not living only in US as well
stand by Peter Jordan, this is the spirit that you can see in many other
countries who respect the Democracy, The Human Rights, The Freedom, and have
been proclamed in the France Revolution, US Constitution as well as in many
other constitutions and UN Declarations.
Again, here in Brazil we're deeply touched by your pain and we hope help you
as better as possible to overcome this terrible incident.


Ubirajara Pereira Rodrigues-Filho



From daemon Mon Sep 17 10:09:56 2001



From: John Olesik :      olesik.2-at-osu.edu
Date: Mon, 17 Sep 2001 11:03:39 -0400
Subject: Electron Microscopist Job Opening at Ohio State University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopist Position

The Ohio State University Microscopic and chemical Analysis Research Center
(MARC) anticipates an opening for an electron microscopist beginning October
1 to operate a scanning electron microscope (SEM) and an electron microprobe
(EPMA). The position includes working with researchers, teaching students
the basic theory and practical SEM and EPMA measurement techniques,
collaborating on research projects and directing staff assistants. We seek
an excellent microscopist who enjoys the exciting atmosphere of a major
research university and will enjoy collaborating with scientists on a broad
variety of projects. The MARC serves the entire Ohio State University as
well as other institutions, with projects from diverse research areas
including geology, materials science, environmental sciences, biological
and biomedical sciences, dentistry, textiles. The MARC focuses on elemental
analysis using from microscale to bulk analysis using SEM, EPMA, inductively
coupled plasma optical emission spectrometry and inductively coupled mass
spectrometry with laser ablation or solution sampling. The MARC also
teaches traditional or short courses on each of the techniques. The
position is a full time, hard money funded job.

If you know of a suitable candidate for this position, please contact Dr.
John Olesik, MARC Director, at olesik.2-at-osu.edu {mailto:olesik.2-at-osu.edu} or
(614) 292-6954. Excellent candidates with a range of experience and
potential to grow will be considered.

----------------------------------------------------------------------------
--
John Olesik
Adj. Assoc. Professor, Research Scientist
MARC Director
Microscopic and chemical Analysis Research Center
Ohio State University
125 S. Oval Mall
275 Mendenhall Laboratory
Columbus, OH 43210

Office: 026D Mendenhall
Phone: (614) 292-6954
FAX: (614) 292-7688
E-mail: olesik.2-at-osu.edu
Web page: www.geology.ohio-state.edu/marc
----------------------------------------------------------------------------
--



From daemon Mon Sep 17 10:33:56 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Mon, 17 Sep 2001 11:24:56 -0400
Subject: Re: my apology - Thank You!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, this is abusing the List. It will be my last abuse, but I could
not leave my inaccurate statement sit.

Thank you for the many emails of support and information.

First. As it was the World Trade Center, along with the large
quantity of Americans, there were many citizens of many countries
that died Tuesday.

Second. Mr. Sinclair was a very real person, and he really did
make those very true statements, albeit back in 1973. If you are
curious, check out:

http://www.rcc.ryerson.ca/schools/rta/ccf/personal/hof/sincla_g.html

Or:

http://www.tysknews.com/Depts/Our_Culture/americans.htm

Darrell



From daemon Mon Sep 17 12:07:57 2001



From: NPGSlithography-at-aol.com
Date: Mon, 17 Sep 2001 12:58:45 EDT
Subject: Re: For the USA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Darrell,

The "article" you forwarded is a hoax in that it was originally broadcast in
1973 and the author Gordon Sinclair died in 1984. However, many will agree
that the ideas in the broadcast apply as well today as they did then.

The original version can be read (and heard) at:

http://www.rcc.ryerson.ca/schools/rta/ccf/news/unique/am_text.html

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Mon Sep 17 12:19:04 2001



From: Norman C Miller :      Norman_C_Miller-at-raytheon.com
Date: Mon, 17 Sep 2001 12:14:18 -0500
Subject: used SEM/EDS available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

We have a used Amray 1000A with a PGT IMIX EDS that we will part with, and
are seeking best offer. The equipment details are on the used equipment
page of the MSA web site.

N. Carl Miller


From daemon Mon Sep 17 12:20:26 2001



From: adam.isle-at-hs-scientific.co.uk ()
Date: Mon, 17 Sep 2001 12:16:10 -0500
Subject: Position Opening - Bristol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: adam.isle-at-hs-scientific.co.uk
Name: Adam Isle

Organization: Hudson Shribman Scientific Recruitment

Location: London, England

I am currently recruiting for a Bristol (South West England) based
high technology company which is seeking an experienced microscopist
to join its pioneering team as a Lab Manager. I would like to know
whether or not you might know of anybody suitably qualified who may
be interested. My client will be happy to arrange Working Visa
details. Regards.

---------------------------------------------------------------------------


From daemon Mon Sep 17 13:06:51 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Mon, 17 Sep 2001 10:59:34 -0700
Subject: RE: SEM imaging on CNT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Haeseong,
If you are just looking at the nanotubes, it would be easy to place some
of the powder on double-sided carbon tape or double sided Cu tape, and just put
the sample in the SEM. The important thing is to have the machine very well
aligned, and astigmatism corrected for a small spot size and a voltage of about
5kV or less. You should start seeing useable information at a magnifcation
range of 30-50kx and above.

-Brad

----------
From: Haeseong Lee
Sent: Monday, September 17, 2001 5:55 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: SEM imaging on CNT

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


??
Hello.

Could anybody recommend the good method of sample preparation for SEM
imaging on carbon nano tubes?
The sample is a powder type including single wall carbon nanotubes
(SWCNTs).
Thank you very much in advance.

Haeseong Lee

{mailto:haeseong-at-hanyang.ac.kr} haeseong-at-hanyang.ac.kr




From daemon Mon Sep 17 19:02:32 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Mon, 17 Sep 2001 17:24:36 -0700
Subject: Re: general method to extract fat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I seriously doubt that Mr. Jordan meant or implied that there were
terrorists in the Listserver.

Much like the lamentations I hear on the radio seemingly directed at
terrorists about how the terrorists have "awakened a sleeping giant" etc. I
doubt the author meant that everyone listening to the radio is a terrorist.

I am sure it was meant as a public message to vent all of our outrage at
this terrible event.

Regards,


Earl Weltmer


----- Original Message -----
} From: "Alwyn Eades" {jae5-at-lehigh.edu}
To: "EMNET" {microscopy-at-sparc5.microscopy.com}
Sent: Monday, September 17, 2001 5:35 AM


I'm not familiar with recent reviews or specific insect protocols, but there's an older book by Morris Kates called techniques in lipidology -part of a series of lab -type manuals. One of the more universal ways to dissolve / extract lipids is the Bligh-Dyer method (Can J Biochem & Physiol 1959 37:911-917 , paraphrased from Kates:

Extract the specimen with one volume of chloroform:methanol:water 1:2:0.8, which efficiently extracts lipids. the extract is then diluted with one volume each of chloroform and water tp form the two-phased system, chloroform and Methanol/water (1.0:0.9). Any water-soluble contaminants are thus partitioned into the methanol-water phase.

Carbon tet is probably NOT the best to extract lipids with -they are more soluble in chloroform

Richard

} "Vr. Richard Bejsak-Colloredo-Mansfeld" {ricardo-at-ans.com.au} 9/17/01 5:37:30 AM } } }
Dear Colleagues

I like to dissolve fat in insects bodies what could be the best chemical
. benzine, carbon tetrachloride, or???

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld



From daemon Mon Sep 17 19:49:31 2001



From: JoAnn Buchanan :      redhair-at-leland.stanford.edu
Date: Mon, 17 Sep 2001 19:43:59 -0500
Subject: lanthanum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello folks. I am trying to fix and stain with lanthanum nitrate and
have followed procedures in Hyatt's book. It mentions that lanthanum
will precipitate in the cold, and when mixed with phosphate buffer.
I have used cacodylate buffer, room temperature solutions and checked
the pH of my buffer(7.2), but I am seeing a lot of ppt in all the
solutions (glut fix, wash and osmium fix). I am wondering if anyone
has any suggestions about how to alleviate this problem. Is the
precipitate harmful to the tissue? Can I filter the solutions to get
rid of big clumps? Is this normal? I would appreciate any feedback
from someone who has used the stuff. Thanks, JoAnn

JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856


From daemon Tue Sep 18 00:40:01 2001



From: Jacob Bastacky :      JBastacky-at-chori.org
Date: Mon, 17 Sep 2001 22:30:31 -0700
Subject: TEM need B&W Print Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone taking down a darkroom or willing to donate a good
photographic processor for black and white prints to a Children's
Hospital?



--
Jacob Bastacky, M.D.
Research Physician
Children's Hospital Oakland Research Institute
5700 Martin Luther King Jr. Way
Oakland, California 94609
Telephone: 510.450.7639
email: jbastacky-at-CHORI.org
FAX: 510.450.7910

Senior Scientist, Visiting
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
sjbastacky-at-lbl.gov


From daemon Tue Sep 18 04:51:29 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Tue, 18 Sep 2001 11:42:47 +0200
Subject: Quest. about Idefix EDS System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear collegues

I would like to hear some opinions about the Idefix EDS system,
commercialized by SamX in France. Are they (probably french) List Members
who know about it ? Please contact me off-list by mail.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Tue Sep 18 10:54:37 2001



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 18 Sep 2001 11:25:58 -0400 (EDT)
Subject: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Hope you are not knocked off from your normal life by the tragic events.

Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
regular sized room. Somehow we feel there is a bit above normal acoustic
echos going on in the lab which interferes with the TEM. So we hope to
find some kind of insulation to be put on the wall and/or around the TEM to
improve the performance.

Any thoughts and suggestions are highly appreciated.

Thanks and take care.

*********************************
Chaoying Ni
Electron Microscopy Facility
College of Engineering
University of Delaware
Newark, DE 19716
*********************************



From daemon Tue Sep 18 11:00:26 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 18 Sep 2001 08:49:54 -0700
Subject: Re: TEM of wool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cathy,
When I was at the Australian Electron Microscopy Meeting there were a lot of
papers about wool, including extensive TEM. Perhapps Mel Gibson or someone
at CSIRO (www.csiro.au) could help.
At 09:55 AM 9/17/01 +1000, you wrote:
} Hi,
}
} Can anyone recommend a method for preparing wool from sheep for TEM? Is it
} easy to section?
}
} Thanks
}
} Cathy Gillespie
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Sep 18 11:05:12 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 18 Sep 2001 08:57:18 -0700
Subject: Re: TEM sample prep: Ti extraction replica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Martin,
Although I have not tried an extraction replica on Ti alloys myself, my
Vander Voort lists several Ti alloy etchants, most based on combinations of
HF asnd other strong mineral acids. The first is:
"20 ml HCl
40 ml HF
40 ml H2O
Immerse specimen in solution at 120 - 150 degrees F for 20-30 min. If smut
forms immerse in 30% H2SO4 for 3 min."
The simplest is:
"50 ml HCl
50 ml H2O
General-purpose macroetch (Ogden anbd Holden)."
Hope this helps.
At 06:40 PM 9/17/01 +0800, you wrote:
} Dear all,
}
} I have been given a piece of titanium that contains small
} precipitates of unknown composition and asked to prepare a TEM sample
} so that the precipitates can be analysed. As it is only the
} precipitates that are of interest I thought that the best approach
} would be to make an extraction replica. However, to do that need to
} find a way to etch away the titanium matrix while leaving behind the
} unknown precipitates.
}
} I would be grateful for any recommendations for a suitable etchant
} that will preferentially remove the titanium matrix so that the
} extraction replica can be made.
}
} Regards,
}
} Martin Saunders.
} --
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Sep 18 11:48:58 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 18 Sep 2001 12:41:47 -0400
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
regular sized room. Somehow we feel there is a bit above normal acoustic
echos going on in the lab which interferes with the TEM. So we hope to
find some kind of insulation to be put on the wall and/or around the TEM to
improve the performance.

Any thoughts and suggestions are highly appreciated.

Dear Chaoying,
One of the other listers will surely know a company which produces
sound-dampening material, but while you wait for it to be delivered, try
using either styrofoam or bubble wrap as a temporary solution. The
styrofoam peanuts would probably work best, but they will take a lot of
labor to install--although you might be able to put up something in front
of the walls and backfill with them. Cloth drapes also work to dampen
sound. Furthermore, you only need to be concerned with the frequency range
which matches resonances in your scope, so when you purchase the
insulation, be sure it will absorb those frequencies. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




From daemon Tue Sep 18 17:32:13 2001



From: NPGSlithography-at-aol.com
Date: Tue, 18 Sep 2001 18:19:34 EDT
Subject: Re: Sinclair broadcast 1973

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Siegfried,

Your comments about the European aviation industry are certainly true and I
would expect that everyone on the list knows about the widespread use of the
Airbus. Consequently, I did not think it was necessary to explain that I did
not mean that the parts of the radio broadcast which include specific
references to the "American technocracy" which are so clearly outdated should
be considered as applicable today.

I completely agree with you that care must be taken regarding references to
the Sinclair broadcast. In fact, in my original posting, I said "many will
agree", which implies that "not all will agree". Also, I referred to the
"ideas" of the broadcast, not the "specifics" of the broadcast. But as your
e-mail points out, the ambiguity of the "ideas" can still lead to confusion,
since they are left to personal interpretation.

Actually, I hesitated to respond to the original posting at all since it was
clearly off-topic, however, the magnitude of the attack and the historical
foundations of the "hoax", made me think that a short clarification regarding
the original radio broadcast may be of general interest. I certainly did not
mean to imply anything negative regarding the European (or any other
country's) patriotism or spirit and I will be more careful regarding postings
in the future that may have any nationalistic interpretations.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Tue Sep 18 17:33:28 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Tue, 18 Sep 2001 17:28:11 -0500
Subject: CSMMS Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For more information or to register, contact Lou Ross at rosslm-at-missouri or
(573) 882-4777.

Central States Microscopy and Microanalysis Society Fall Meeting
Friday, October 19, 2001
Adams Conference Center
Veterinary Medicine Building
University of Missouri
Columbia, Mo 65211

8 - 8:45 Registration

8:50 Welcome

9:00 Tobias Baskin, University of Missouri-Columbia, "Ultrastructure of the
Plant Cell Wall Analyzed with Field-Emission Scanning Electron Microscopy"

9:15 Nadia Navarrete-Tindall, University of Missouri-Columbia, "Morphology
of the Endangered Legume Amorpha nitens Boynton and its Rhizobia Symbiont"

9:30 Heide Schatten, University of Missouri-Columbia, "Cytoskeletal
Dynamics in Apicomplexan Parasites"

9:45 Joe Simmons, University of Missouri-Columbia, "Idiopathic Interstitial
Pneumonia in Laboratory Rats"

10:00 Marty Katz, University of Missouri-Columbia, "Stem Cell
Transplantation Therapy for Inherited Neurodegenerative Disorders"

10:15 Tom Phillips, University of Missouri-Columbia, "Fixation of
Biological Tissues - Science or Art?"

10:30 - 10:45 BREAK

10:45 Robert Hall, Associate Vice Provost of Research, University of
Missouri-Columbia, "The University of Missouri: Taking the Lead in National
Research Funding"

11:00 Keynote speaker: Kenneth Moore, University of Iowa, MSA Tour Speaker,
"Applications of Microscopy Techniques to the Study of Genetic Therapy
Research"

12:00 - 1:30 Lunch

1:30 John Bozzola, SIU-Carbondale, "Multidisciplinary Approach to Teaching
Electrion Microscopy"

1:45 Howard Wilson, University of Missouri-Columbia, "Creating a Scientific
Poster Presentation using Microsoft Powerpoint"

2:00 Scott Walck, PPG, "Low Angle Cleavage - A New Specimen Preparation
Method for TEM"

2:15 Louis Ross, University of Missouri-Columbia, "Exciting Times in
Scanning Electron Microscopy and X-ray Microanalysis"

2:30 Vladimir Dusevich, University of Missouri-KC, "Practical ESEM"

2:45 Jeff Speakman, University of Missouri-Columbia, "Laser Ablation ICP-MS
as a Tool for Characterization of Archaeological Materials"

3:00 - 3:15 BREAK

3:15 Cammy Bright, University of Missouri-Columbia, "A Laser Ablation
ICP-MS and SEM Study of Rare Earth and Trace Elements in Conodonts"

3:30 Mike Amspoker, Westminster College, "A Light and Scanning Electron
Microscopy Study of the Freshwater Diatom Desmogonium rabenhorstianum
Grunow"

3:45 Heather Ramsay, University of Missouri-Columbia, "New Applications of
SEM in Osteological Morphology Research"

4:00 Dickerson Moreno, University of Missouri, "Development of N-Type
Diamond Semiconductor Through Field Enhanced Diffusion by Optical
Activation"

4:15 Alejandro Suarez, University of Missouri, "A New Method to Fabricate
Polycrystalline Diamond as a P-Type Semiconductor"

4:30 Closing Remarks

4:45 CSMMS Business Meeting







Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.missouri.edu/~geosclmr/ebaf.html


From daemon Tue Sep 18 19:45:27 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 19 Sep 2001 12:32:46 GMT+1200
Subject: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello, friends

I've just had the misfortune to smash the glass bell jar on my
Edwards 306 coater. About 305mm dia, 400mm high.

While I can and maybe should buy a glass replacement, I'm tempted to
make one from a 400mm length of 305mm diameter stainless-steel pipe
with a flat top of 10mm thick polycarbonate.

I've never liked having my face too close to the glass one when it's
in use, anyway.

Anyone got any comments/recommendations?

Anyone ever had a glass one implode in use?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Sep 19 02:18:48 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 19 Sep 2001 08:15:22 +0100 (GMT Daylight Time)
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ritchie,

I have not known a glass tube to implode but we use an
outer cover just in case (perspex tube). Tubes are checked
for cracks before use and previous problems have been
obvious, usually by the many bits all over the floor!

My worry would be the 10mm polycarbonate top, at 300mm dia
that's over 1500 pounds (750Kg?) force. I would want
something thicker or stronger.

I also like being able to look in the side to see my
coating set up after evacuation and the evaporation
(through appropriate goggles).

Regards,
Ron

} I've just had the misfortune to smash the glass bell jar on my
} Edwards 306 coater. About 305mm dia, 400mm high.
}
} While I can and maybe should buy a glass replacement, I'm tempted to
} make one from a 400mm length of 305mm diameter stainless-steel pipe
} with a flat top of 10mm thick polycarbonate.
}
} I've never liked having my face too close to the glass one when it's
} in use, anyway.
}
} Anyone got any comments/recommendations?
}
} Anyone ever had a glass one implode in use?
}
} cheers
}
} rtch
}
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Sep 19 02:31:44 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 19 Sep 2001 08:34:08 +0100 (GMT Daylight Time)
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chaoying,

There are two sources of noise, externally and internally
generated. You have specificcally asked about echos so I
assume that your problems stem from the rotary pumps,
cooling fans, etc. on equipment that is essential to have
operating in the room.

Try to get the rotary pumps and power supply rack into an
area that can be screened off from the instrument column
but remember that it will still need air flow for cooling.

For the desk and computer fans use some blackout curtains
around the walls. This is readily available and is usually
black one side and coloured the other so you can have a
nice Oxford blue showing into the room:-) Make sure that it
is at least 1.5 lengths, preferably 2x - ie. 7.5m of
curtaining is a minimum for a 5m wall 10m is best. If you
get the rotary pump and power supply at the back of the
room you can hide them behind 3 layers of the same
curtaining.

We have found this very effective for our HREM instruments
and our FEGTEM.

Noise from outside the room needs dealing with outside the
room.

Regards,
Ron

} Dear Listers,
}
} Hope you are not knocked off from your normal life by the tragic events.
}
} Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
} regular sized room. Somehow we feel there is a bit above normal acoustic
} echos going on in the lab which interferes with the TEM. So we hope to
} find some kind of insulation to be put on the wall and/or around the TEM to
} improve the performance.
}
} Any thoughts and suggestions are highly appreciated.
}
} Thanks and take care.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Facility
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Sep 19 03:35:21 2001



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Wed, 19 Sep 2001 09:27:07 +0100
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chaoying,

We have a 2010F in a similar sized room and we use floor to ceiling
black curtaining - the heavier the better (ours are double thickness
and lined) - and it makes a big difference.
Hope this helps
Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365 Mob: 07796 055149
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************


From daemon Wed Sep 19 07:30:14 2001



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 19 Sep 2001 08:18:26 -0400
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie,

Don't use a polycarbonate end plate to cover the end of your vacuum
chamber. A flat plate of polycarbonate isn't strong enough to span the
opening.

Edwards did sell glass cylinders with metal endplates on some of their 306
coaters--in particular their old ion beam sputtering system. The unit I
have here has a 1/2 inch thick Aluminum plate spanning a 12 inch diameter
glass vacuum cylinder. The glass cylinder is the same type of glass you
find on a normal bell jar. It just has an L-gasket on both the top and
bottom. Be careful that you use an adequate grade of aluminum for the top
plate.

The advantage of the cylinder with metal top plate is that you can have
feedthroughs to the vacuum chamber from the top as well as through the base
plate.

I suspect that you can get the glass cylindrical glass vacuum vessels and
L-gaskets from the usual vacuum supply houses.

cheers,
Henk Colijn

At 12:32 PM 9/19/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Fools are pleased when they discover error.
The wise are pleased when they discover truth.



From daemon Wed Sep 19 09:24:21 2001



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 19 Sep 2001 09:59:59 -0400
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Hi Ritchie,

I have not known a glass tube to implode but we use an
outer cover just in case (perspex tube). Tubes are checked
for cracks before use and previous problems have been
obvious, usually by the many bits all over the floor!

My worry would be the 10mm polycarbonate top, at 300mm dia
that's over 1500 pounds (750Kg?) force. I would want
something thicker or stronger.

I also like being able to look in the side to see my
coating set up after evacuation and the evaporation
(through appropriate goggles).

Regards,
Ron

} I've just had the misfortune to smash the glass bell jar on my
} Edwards 306 coater. About 305mm dia, 400mm high.
}
} While I can and maybe should buy a glass replacement, I'm tempted to
} make one from a 400mm length of 305mm diameter stainless-steel pipe
} with a flat top of 10mm thick polycarbonate.
}
} I've never liked having my face too close to the glass one when it's
} in use, anyway.
}
} Anyone got any comments/recommendations?
}
} Anyone ever had a glass one implode in use?
}
} cheers
}


Dear Ritchie,

I have a polycarbonate lid for a vacuum apparatus with the same
diameter as your coater. It is about 45 mm thick, and it has not
given me any trouble. I agree with Ron that 10 mm is not thick
enough.

Yours,


Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us



From daemon Wed Sep 19 09:55:35 2001



From: Russell E. Cook :      recook-at-anl.gov
Date: Wed, 19 Sep 2001 09:48:53 -0600
Subject: Re: How to insulate noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying:

We've had to contend with noisy rooms in nearly all of our electron
microscope laboratories. Here is a list of things to do:

* Try to eliminate the source of the noise.
* Call in the microscope manufacturer to provide a noise spectrum (dB
versus Hz). Ask which frequencies are a problem (usually low frequencies).
* Look for devices and materials which will attenuate the noise. Pay
particular attention to their effectiveness in attenuating the problem
frequencies.

Here are a few companies which manufacture items you may need:
* Kinetics Noise Control, http://www.kineticsnoise.com
* Industrial Acoustics Company, http://www.industrialacoustics.com
* Industrial Noise Control, http://www.industrialnoisecontrol.com

I've worked several times with David Mitchell, VP at The Huff Co., Lake
Bluff, IL (Chicago area), 1-847-362-7440, to ameliorate our noise problems.
Such companies will evaluate the site, recommend solutions, and even
install the various devices and materials from the manufacturers.

Some of the things we've done are:
* glued "egg-crate" foam on the walls
* installed fabric-covered fiberglass panels on the walls
* installed duct silencers in air ducts
* wrapped air ducts with fiberglass batting backed with "loaded vinyl"
* hung fiberglass noise-absorbing curtains
* installed isolation pads under vibrating equipment

If you install wall panels or foam, it is usually not necessary to cover
all of the wall surfaces. There are lots of things to consider, so
evaluate your site carefully.

..Russ Cook
----------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov

} -----------------------------------------------------------------------.
} Dear Listers,
}
} Hope you are not knocked off from your normal life by the tragic events.
}
} Anyway, I have a question for you gurus: we have a 2010F TEM sitting in a
} regular sized room. Somehow we feel there is a bit above normal acoustic
} echos going on in the lab which interferes with the TEM. So we hope to
} find some kind of insulation to be put on the wall and/or around the TEM to
} improve the performance.
}
} Any thoughts and suggestions are highly appreciated.
}
} Thanks and take care.
}
} *********************************
} Chaoying Ni
} Electron Microscopy Facility
} College of Engineering
} University of Delaware
} Newark, DE 19716
} *********************************





From daemon Wed Sep 19 10:39:15 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 19 Sep 2001 11:26:59 +0200
Subject: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it common to use mouse antibodies (monoclonals) on mouse tissue for
immunogold localizations? Is a control using the secondary antibody
(anti-mouse-IgG-gold-conjugate) alone sufficient to make sure that one
is detecting specificly the primary antibody and not tissue-internal
mouse antibodies?
Does somebody know examples from the literature where people used mouse
antibodies (monoclonals) on mouse tissue for immunogold localizations?
I am a botanist, so until now I never ran into that sort of problems.

Thanks,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
Universität zu Köln
Botanisches Institut
Gyrhofstr. 15
50931 Köln
Germany
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 19 11:04:57 2001



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 19 Sep 2001 11:00:52 -0500
Subject: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I didn't get to the MSA meeting this summer, but I heard through the
grapevine about Gatan's new UltraScan 1000 and saw their poster. I was very
impressed with the 4K x 4k montage taken at 5k mag. It looked
publishable.I'd be interested in hearing comments/impressions from anyone
who a chance to use it.
Thanks,

Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Wed Sep 19 11:20:09 2001



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Wed, 19 Sep 2001 12:09:32 -0400
Subject: Re: How to insulat noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chaoying,

We are about to install a Tecnai 20 TEM. In addition to isolating rotary
pumps and power supply as suggested by Ron Doole, we are covering the walls
with Armstrong Sound Soak Panels.

Frank

At 11:25 AM 9/18/01 -0400, Chaoying Ni wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Sep 19 12:28:57 2001



From: Stefan Geimer :      stefan.geimer-at-yale.edu
Date: Wed, 19 Sep 2001 13:17:01 +0200
Subject: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it common to use mouse antibodies (monoclonals) on mouse tissue for
immunogold localizations? Is a control using the secondary antibody
(anti-mouse-IgG-gold-conjugate) alone sufficient to make sure that one
is detecting specificly the primary antibody and not tissue-internal
mouse antibodies? Or are there other things I can doo as a good control?

Does somebody know examples from the literature where people used mouse
antibodies (monoclonals) on mouse tissue for immunogold localizations?
I am a botanist, so until now I never ran into that sort of problems.

Thanks,

Stefan


°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Stefan Geimer
Universität zu Köln
Botanisches Institut
Gyrhofstr. 15
50931 Köln
Germany
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°




From daemon Wed Sep 19 12:31:33 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 19 Sep 2001 13:29:42 -0400
Subject: BELL JAR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to the person from down under with the broken bell jar:

I have been using a Denton 502A carbon evaporator for the past decade and
have also had some visions of it imploding. The model I have has a metal
mesh cover over the jar and while it would not stop little pieces of flying
glass it would catch the big ones. As far as using a home made unit goes, I
would be vary cautious. At the very least consult with some kind of
structural engineer who is familiar with vacuum systems. Be careful. Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Wed Sep 19 14:46:29 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Wed, 19 Sep 2001 15:57:40 -0400
Subject: Re: How to insulat(e) noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sara, actually the total package for the UltraScan 1000 is about $100K. The
UltraScan 4000 is the one you've quoted and that is a 4kx4k camera. The 1000
has the 2kx2k camera and montages the field requiring 4 exposures. I believe
that is the way it works.

Hank

-----Original Message-----
} From: Sara Miller [mailto:saram-at-duke.edu]
Sent: Wednesday, September 19, 2001 1:58 PM
To: Hank Adams


Dear Chaoying,
One unlikely source of noise we recently found came from the
diffusion pump on our CM 300. It was crackling like diffusion pumps
due, but we could see our HRTEM image shake with the big crackles.
The solution was deduced by our excellent FEI field engineer. It was
to reduce the temperature in the diffusion pump by reducing the
amount of oil charge. If you have noise problems that go snap,
crackle, pop in the dark, try checking your oil.
Ciao for now,
Ken


From daemon Wed Sep 19 18:53:38 2001



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Wed, 19 Sep 2001 18:45:24 -0500
Subject: Re: How to insulat(e) noise in EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chaoying,
One unlikely source of noise we recently found came from the
diffusion pump on our CM 300. It was crackling like diffusion pumps
due, but we could see our HRTEM image shake with the big crackles.
The solution was deduced by our excellent FEI field engineer. It was
to reduce the temperature in the diffusion pump by reducing the
amount of oil charge. If you have noise problems that go snap,
crackle, pop in the dark, try checking your oil.
Ciao for now,
Ken


From daemon Wed Sep 19 19:44:56 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 17:39:29 -0700
Subject: Re: Vacuum Coater Bell Jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


10 mm polycarbonate? I would suggest much thicker. Have no idea how much
thick. I think the problem is that your pipe has a huge diameter. The
force on your polycarbonate will be proportional to the size of area (1
kg/cm2 x 706 cm2=approx 700 kg, 1600 lb). I afraid, the total cost of the
pipe etc would be comparable to the cost of Bell Jar. You may find Bell
Jar on used equipment sale for about $300.
Sergey

At 12:32 PM 9/19/01 +0000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 19 19:56:52 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 17:51:05 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is incredible. I would suggest that this is a first EM digital camera
which produces comparable to the film image quality. The chip is huge, so
they have 80-90% area of the film on the chip. The images are very sharp.
The technical problem there was to read information from such huge
chip. They used new technology when chip (virtually?) splitted on quarters
and read in parallel. They did a great job. The camera is very expensive
by the way.

Sergey

At 11:00 AM 9/19/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Sep 19 20:14:55 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Sep 2001 18:09:46 -0700
Subject: RE: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hank,

I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
real number of pixels on the chip. I also believe, Ultrascan1000 is much,
much more expensive than you suggest. As per our conversation with Gatan
people, they expect to start selling that cameras at the end of the year,
so it's very unlikely the price is known for now (I did not ask them
directly about the price, but have impression that it's much more expensive
that previous models, which were in the $70K range). Current Gatan's
cameras (600W/CW Multiscan for instance) already has capacity for multiple
exposure I believe. Actually, it's not a properties of camera but
software. It's called "Auto-montage" and you could "montage" as many
pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
exact number) bigger area of view than film, which sometime is very
useful. Over last few month I was studying EM digital cameras on the
makket very hard, so if you have questions, may be I could help.

Sergey.

At 02:42 PM 9/19/01 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 06:40:30 2001



From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Thu, 20 Sep 2001 13:30:57 +0200
Subject: TEM: fluorescence micrsocopy and TEM on same sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate any advice on the following question. I am planning to do
fluorescence microscopy and electron microscopy on the same sample. I would
like to do immunolabelling on cells grown in a culture dish followed by plastic
embedding of the same sample and further preparation for EM.
In order to find the right area of cells to be sectioned for EM (previously
detected by immuno fluorescence) I suppose one has to have some kind of grid
on the culture dish. I am aware of the fact that there are gridded coverslips
available, however, I always had a lot of trouble to remove the coverslip from
the glass after embedding (used lN2 and so on). So it would be very helpful if
anyone out there could provide any other working recipes, references for
methods or sources for culture dishes with some kind of grid on them.


Dr. Andreas Brech
EM-Unit, Dept. of Biology,
University of Oslo.
P.O.Box 1062 Blindern, Room U 139
Street address: Moltke Moes vei 32
N-0316 Oslo 3
Norway
Tel.: + 47-22 85 47 25/61 89 (work)
+ 47-22 43 83 23 (privat)
Fax.: + 47-22 85 47 26
e-mail.: abrech-at-bio.uio.no


From daemon Thu Sep 20 06:51:52 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Thu, 20 Sep 2001 12:47:31 +0100
Subject: LM - acridine orange - help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Listers

I have a visitor who wants to use acridine orange (0.1%) in seawater (or artificial seawater) to stain nuclei/DNA in limpet eggs and sperm during fertilisation. It is done by other workers, but so far we've found no information as to how long it takes to stain things. Any suggestions? Any pH considerations?

Also, does it work on fresh (i.e. marine, seawater)and formalin-fixed material with equal efficiency.

Finally, we understand that the fluorescence is short-lived - any comments as to how long a time period would be helpful.


Many thanks

Keith Ryan
Marine Biological Association
& University of Plymouth
UK

PLymouth



From daemon Thu Sep 20 07:00:43 2001



From: DrJohnRuss-at-aol.com
Date: Thu, 20 Sep 2001 07:56:12 EDT
Subject: 2nd Announcement: Image Processing and Measurement Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The three-day intensive hands-on workshop on Image Processing and Measurement
presented by John Russ through the North Carolina State University Department
of Continuing and Professional Education is now in its 19th year. The course
will be presented October 30 - November 1, in Raleigh, NC. This course has
generated highly favorable reviews from the thousands of previous students.
The primary focus is on images from various types of microscopy, with
practical guidance in correcting imaging defects, enhancing the images for
presentation and measurement, and performing stereological meaningful
measurements on them. Textbooks and computer software are provided to
attendees. Lab sessions with an opportunity to bring your own images makes
this course immediately useful and highly productive.

For full information on the course, including outlines, faculty information,
a downloadable brochure, and on-line registration, go to

http://members.AOL.com/IPCourse/

or call Cindy Allen, 919-515-8171, at North Carolina State University Dept.
of Continuing and Professional Education.


From daemon Thu Sep 20 11:07:14 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 20 Sep 2001 10:37:47 -0500
Subject: Enviornmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

I am gathering data on enviornmental SEMs. Hopefully I'll be setting up a
research facility within the coming months.

Does anyone have preference to systems?

What, if any differences are there for maintinence compared to a standard
SEM?

COST? (ouch)

Thanks,

Tim Quinn
University of Kansas
Natural History Museum &
Biodiversity Research Center
1345 Jayhawk Blvd 414A
Lawrence, KS 66045
785-864-4556
tquinn-at-ku.edu


From daemon Thu Sep 20 11:39:53 2001



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Thu, 20 Sep 2001 09:37:11 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That *is* incredible.
Chip has length of 4080 pixels by 15 micron pixel pitch = 61.2 mm. Area is then
37.45 square cm or 5.8 square inches. Plate (film) size is 3.25 inches by 4.00
inches = 13 square inches. Chip area is 45% of film area...
-Mike

Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This is incredible. I would suggest that this is a first EM digital camera
} which produces comparable to the film image quality. The chip is huge, so
} they have 80-90% area of the film on the chip. The images are very sharp.
} The technical problem there was to read information from such huge
} chip. They used new technology when chip (virtually?) splitted on quarters
} and read in parallel. They did a great job. The camera is very expensive
} by the way.
}
} Sergey
}
} At 11:00 AM 9/19/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I didn't get to the MSA meeting this summer, but I heard through the
} } grapevine about Gatan's new UltraScan 1000 and saw their poster. I was very
} } impressed with the 4K x 4k montage taken at 5k mag. It looked
} } publishable.I'd be interested in hearing comments/impressions from anyone
} } who a chance to use it.
} } Thanks,
} }
} } Hank Adams
} } Integrated Microscopy Core
} } Molecular and Cellular Biology
} } Baylor College of Medicine
} } Houston, Tx 77030
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu



From daemon Thu Sep 20 12:44:24 2001



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Thu, 20 Sep 2001 13:39:02 -0400
Subject: NYSEM Presidential Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The New York Society of Experimental Microscopists
Announcing the 50th Anniversary
Presidential Symposium

"FRONTIERS in MICROSCOPY"

Caspary Auditorium, Rockefeller University
October 11, 2001 9:00 AM-4:00 PM
Free and open to the Public

50 Years of NYSEM
C. DeLemos-Chiarandini, New York University

George Palade, and the Early Days of Electron Microscopy in New York
P. Satir, Albert Einstein College of Medicine

EM Tomography Reveals Cell Structure at 6nm Resolution
J. R. McIntosh, University of Colorado

Multiphoton Imaging of the Molecular Dynamics of Life Processes
W.Webb, Cornell University

Intravital Imaging of Tumor Cells
J.Condeelis, Albert Einstein College of Medicine

Total Internal Reflectance Microscopy Visualizes Membrane Fusion Events
S. Simon, Rockefeller University

Imaging Biochemistry Inside Cells
P. Bastiaens, EMBL, Heidelberg

The Ribosome: Snapshots of a Molecular Machine in Motion
J. Frank, HHMI, Wadsworth Center

Imaging RNA in Living Cells
R. Singer, Albert Einstein College of Medicine

High Temporal and Spectral Resolution using Acousto-Optical Tunable Filters
D. Farkas, Carnegie-Mellon University

****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************



From daemon Thu Sep 20 12:52:12 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Thu, 20 Sep 2001 12:41:07 -0500
Subject: Re: TEM: fluorescence micrsocopy and TEM on same sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Brech:

You can culture your cells on plastic coverslips and embed them in resin. The
embedded cells are good for both IFM and iEM but you have to work out the
conditions. The coverslip is called Thermanox from EMS.

Greg


Gang Ning, M.D., Ph.D.
Electron Microscopy Facility
Medical College of Wisconsin

Andreas Brech wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would appreciate any advice on the following question. I am planning to do
} fluorescence microscopy and electron microscopy on the same sample. I would
} like to do immunolabelling on cells grown in a culture dish followed by plastic
} embedding of the same sample and further preparation for EM.
} In order to find the right area of cells to be sectioned for EM (previously
} detected by immuno fluorescence) I suppose one has to have some kind of grid
} on the culture dish. I am aware of the fact that there are gridded coverslips
} available, however, I always had a lot of trouble to remove the coverslip from
} the glass after embedding (used lN2 and so on). So it would be very helpful if
} anyone out there could provide any other working recipes, references for
} methods or sources for culture dishes with some kind of grid on them.
}
} Dr. Andreas Brech
} EM-Unit, Dept. of Biology,
} University of Oslo.
} P.O.Box 1062 Blindern, Room U 139
} Street address: Moltke Moes vei 32
} N-0316 Oslo 3
} Norway
} Tel.: + 47-22 85 47 25/61 89 (work)
} + 47-22 43 83 23 (privat)
} Fax.: + 47-22 85 47 26
} e-mail.: abrech-at-bio.uio.no



From daemon Thu Sep 20 15:17:08 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Thu, 20 Sep 2001 16:08:46 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listers,
I have a question about a schottky tipped FE Microscope. Specifically, what
is the best way to shut the beam off at the end of the day. We will be using
probably everyday for long sessions, so would beam blanking be preferable to
shutting off the extraction voltage. I am wondering about the effects of tip
life, beam stability, and contamination. Any advice or past experience would
be greatly appreciated.
Thanks again
Nick Aitken

Nicol Aitken
Sample Preparation and Imaging Specialist
Research and Development
Semiconductor Insights Inc.
email:nicol-at-semiconductor.com
(613)599-6500 ext.4300


From daemon Thu Sep 20 16:47:12 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 20 Sep 2001 14:41:01 -0700
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ingo

Yes, I did check your WEB-site in the beginning of my search a few month
ago as well as many others. My initial criteria were the following:

-35 mm port mount;
-price;
-ability to have demo for the camera;
-ability to have reasonable service in time.

You product was excluded from initial list for the following reasons:

- your Biocam camera, which was possible candidate is bottom-mounted;

- on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
any information about your headquarter in US or contact phones, so I was
not able to talk to your representative (I was using the telephone call as
a first "try" of the customer service quality: if I was on hold for more
than 5 min, I excluded the candidate from my list).

-location of your head office in Germany makes me skeptical about customer
service quality company could provide in US.

I would like to mention that my criteria is subjective and based on my own
way to make a business and spent my money. My decision is not related to
the real quality of your products.

I would mention also that a few companies refused to give me demo on their
cameras (or delayed with answer) and were excluded from the list as well.

Sergey.

At 02:30 PM 9/20/01 +0200, you wrote:
} Dear Mr. Sergey Ryazantsev,
}
} I have read in your email, that you have studied cameras for TEM.
} Have you studied our cameras as well?
}
} For example, this year we have had the following installations in California:
} - Scripps in San Diego, F-224 at a CM-200FEG (Ron Milligan's lab)
} - UC in San Diego, F-224/FastScan at a JEM-200EX (Mark Ellisman's lab)
} - Quantum Dot Corporation in Hayward, TemCam-0124 at JEM-200CX
} - Additionally just this month Bridget Carragher (together with her group) has
} moved from Illinois to the Scripps Institute. She will mount another F-224
} at a
} Tecnai-20FEG.
}
} If you want to know more about our US installations and our cameras,
} please let
} me know.
}
} Best wishes,
} Ingo Daberkow
}
} BTW: We are in contact with Phoebe Stewart from the Department of Molecular &
} Medical Pharmacology, UCLA School of Medicine
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Dr. Ingo Daberkow
} Tietz Video and Image Processing Systems GmbH
} Herbststrasse 7
} D-82131 Gauting, Germany
} Tel: +49-89-8506567
} FAX: +49-89-8509488
} Internet: http://www.tvips.com/
} Email: ingo.daberkow-at-tvips.com
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
}
} Sergey Ryazantsev wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hank,
} }
} } I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
} } real number of pixels on the chip. I also believe, Ultrascan1000 is much,
} } much more expensive than you suggest. As per our conversation with Gatan
} } people, they expect to start selling that cameras at the end of the year,
} } so it's very unlikely the price is known for now (I did not ask them
} } directly about the price, but have impression that it's much more expensive
} } that previous models, which were in the $70K range). Current Gatan's
} } cameras (600W/CW Multiscan for instance) already has capacity for multiple
} } exposure I believe. Actually, it's not a properties of camera but
} } software. It's called "Auto-montage" and you could "montage" as many
} } pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
} } exact number) bigger area of view than film, which sometime is very
} } useful. Over last few month I was studying EM digital cameras on the
} } makket very hard, so if you have questions, may be I could help.
} }
} } Sergey.
} }
} } At 02:42 PM 9/19/01 -0500, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Sara, actually the total package for the UltraScan 1000 is about
} $100K. The
} } } UltraScan 4000 is the one you've quoted and that is a 4kx4k camera.
} The 1000
} } } has the 2kx2k camera and montages the field requiring 4 exposures. I
} believe
} } } that is the way it works.
} } }
} } } Hank
} } }
} } } -----Original Message-----
} } } } From: Sara Miller [mailto:saram-at-duke.edu]
} } } Sent: Wednesday, September 19, 2001 1:58 PM
} } } To: Hank Adams
} } } Subject: Re: Gatan's new UltraScan 1000
} } }
} } }
} } } Micrograph was lovely. Price tag was in range of $275 K--ouch! They
} didn't
} } } have one there on demo that I saw.
} } }
} } } Sara E. Miller, Ph. D.
} } } P. O. Box 3712
} } } Duke University Medical Center
} } } Durham, NC 27710
} } } Ph: 919 684-3452
} } } FAX: 919 684-3265
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 16:47:48 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 20 Sep 2001 14:44:00 -0700
Subject: RE: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hank
Thanks for the comment.

Steve Pfeiffer of Gatan just E.mail to me that price range for Ultrascan
1000 is about $100K. Which is much less than I expect to see for such camera.

Sergey

At 10:06 AM 9/20/01 -0500, you wrote:
} Sergey, I just talked to Gatan about their UltraScan 1000 that tiles 4
} images together. The entire package with installation/training and the
} Automontage plug-in minus the computer/monitor is about $100K. Using their
} specs for the computer add another $4800 which includes a 21" monitor. The
} CCD magnifies the image 1.3x-1.5x compared to film.
}
} Hank
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, September 19, 2001 8:10 PM
} To: Hank Adams; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Gatan's new UltraScan 1000
}
}
} Hank,
}
} I think you wrong. They do have two new cameras 2x2K and 4x4K. This is
} real number of pixels on the chip. I also believe, Ultrascan1000 is much,
} much more expensive than you suggest. As per our conversation with Gatan
} people, they expect to start selling that cameras at the end of the year,
} so it's very unlikely the price is known for now (I did not ask them
} directly about the price, but have impression that it's much more expensive
} that previous models, which were in the $70K range). Current Gatan's
} cameras (600W/CW Multiscan for instance) already has capacity for multiple
} exposure I believe. Actually, it's not a properties of camera but
} software. It's called "Auto-montage" and you could "montage" as many
} pictures as you want. By the way, Multiscan cameras has 2-4x (not sure in
} exact number) bigger area of view than film, which sometime is very
} useful. Over last few month I was studying EM digital cameras on the
} makket very hard, so if you have questions, may be I could help.
}
} Sergey.
}
} At 02:42 PM 9/19/01 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Sara, actually the total package for the UltraScan 1000 is about $100K. The
} } UltraScan 4000 is the one you've quoted and that is a 4kx4k camera. The
} 1000
} } has the 2kx2k camera and montages the field requiring 4 exposures. I
} believe
} } that is the way it works.
} }
} } Hank
} }
} } -----Original Message-----
} } } From: Sara Miller [mailto:saram-at-duke.edu]
} } Sent: Wednesday, September 19, 2001 1:58 PM
} } To: Hank Adams
} } Subject: Re: Gatan's new UltraScan 1000
} }
} }
} } Micrograph was lovely. Price tag was in range of $275 K--ouch! They
} didn't
} } have one there on demo that I saw.
} }
} } Sara E. Miller, Ph. D.
} } P. O. Box 3712
} } Duke University Medical Center
} } Durham, NC 27710
} } Ph: 919 684-3452
} } FAX: 919 684-3265
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Thu Sep 20 17:04:22 2001



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 20 Sep 2001 17:59:41 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nicol,

For the past 4 years we have run the thermal emitter in our Zeiss LEO
DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
emitter (from FEI) has 3,000 hours since November 2000. We routinely image
at } 100kx with great results.
What microscope do you have?

Jim







Nicol Aitken wrote:

Hi Listers,
I have a question about a schottky tipped FE Microscope. Specifically, what
is the best way to shut the beam off at the end of the day. We will be using
probably everyday for long sessions, so would beam blanking be preferable to
shutting off the extraction voltage. I am wondering about the effects of tip
life, beam stability, and contamination. Any advice or past experience would
be greatly appreciated.
Thanks again
Nick Aitken

Nicol Aitken
Sample Preparation and Imaging Specialist
Research and Development
Semiconductor Insights Inc.
email:nicol-at-semiconductor.com
(613)599-6500 ext.4300

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Fri Sep 21 06:00:29 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 21 Sep 2001 11:58:12 +0100 (GMT Daylight Time)
Subject: Re: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Nicol and Jim,

There may be a difference in TEM and SEM FEG use that I am
not familiar with, I am primarily a TEM guy, however we
keep our emission running as much as possible.

With our JEOL3000F FEGTEM we run the tip down maybe 4 times
a year, other than that it is on and the valve between the
gun and the column is closed when the instrument is not
being used. There are several reasons for this.

It takes a couple of hours to run up and condition the HT
at 300kV and then another couple of hours to run up the
emission. This makes it impossible to switch on the
emission for a day's use.

Having run up the emission it is not stable, the emission
current keeps dropping. This exponential decrease takes
several hours to stabilise (we allow 6 hours before we
think it is really stable). Of course if you don't need a
stable current (eg. for HREM imaging) you can still use it.

The most likely time for any damage to the tip (apart fom
accidents) is when it is heated or cooled so we don't want
to do that more than neccessary.

I guess that there is finite life of the tip so it would be
pointless to keep it running for a long time if the
instrument is not being used. Does anyone know why a tip
should eventually fail if operating conditions are
optimised?

Our present tip (the first) has been running during factory
tests and since installation in Oxford in 1998 and has run
for over 21,500 hours.

Regards,
Ron
ps. If our tip now fails I'm going to blame you guys for
raising the subject.



} Nicol,
}
} For the past 4 years we have run the thermal emitter in our Zeiss LEO
} DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
} previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
} emitter (from FEI) has 3,000 hours since November 2000. We routinely image
} at } 100kx with great results.
} What microscope do you have?
}
} Jim
}
}
} Hi Listers,
} I have a question about a schottky tipped FE Microscope. Specifically, what
} is the best way to shut the beam off at the end of the day. We will be using
} probably everyday for long sessions, so would beam blanking be preferable to
} shutting off the extraction voltage. I am wondering about the effects of tip
} life, beam stability, and contamination. Any advice or past experience would
} be greatly appreciated.
} Thanks again
} Nick Aitken
}
} Nicol Aitken
} Sample Preparation and Imaging Specialist
} Research and Development
} Semiconductor Insights Inc.
} email:nicol-at-semiconductor.com
} (613)599-6500 ext.4300
}
} James S. Romanow
} The University of Connecticut
} Physiology and Neurobiology Department
} Electron Microscopy Facility
} Unit-2131
} Storrs, CT 06269-2131
} bsgphy3-at-uconnvm.uconn.edu
} 860 486-2914 voice
} 860 486-1936 fax
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri Sep 21 07:33:02 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 21 Sep 2001 08:23:56 -0400
Subject: RE: LM - acridine orange - help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning (for me!) Keith,

For the most complete information on fluorescence probes (which you may
already know), try URL: http://www.probes.com/; Search: "Acridine Orange"
for a plethora (my son accused me of a "plethora of harassment", the other
night!) of information and citations.

You might also consult Hayat, MA, Stains and Cytochemical Methods, Plenum,
1993, ISBN: 0-306-44294-9 for an informative, though not explicit,
description of AO and its applications and properties.

For the reason that AO has been used extensively in flow cytometry, you
should also look into the several chapters that are relevant to your
question in: Methods in Cell Biology, vol 33, Flow Cytometry, Darzynkiewicz
and Crissman(Eds.)Academic Press, 1990, ISBN: 0-12-564133-8 or
0-12-203050-8.

pH is important to the fluorescent properties of the dissolved dye, but I
have not observed such effects in my experience which has been confined to
viewing virus-infected cultured cells (vital, but usually fixed/dead). My
original histochemical use of AO fluorescence - early '60's - was to
distinguish between DNA and RNA - until it was pointed out by virologists
that there were some single-stranded DNA viruses that could interfere with
interpretation. Now we know that AO can be used to intercalate
preferentially in transcribing DNA, thus providing an indication of RNA
production.

I hope this helps, and

Good luck,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Keith Ryan
} Reply To: kpr-at-mba.ac.uk
} Sent: Thursday, September 20, 2001 7:47 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM - acridine orange - help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello Listers
}
} I have a visitor who wants to use acridine orange (0.1%) in seawater (or
} artificial seawater) to stain nuclei/DNA in limpet eggs and sperm during
} fertilisation. It is done by other workers, but so far we've found no
} information as to how long it takes to stain things. Any suggestions? Any
} pH considerations?
}
} Also, does it work on fresh (i.e. marine, seawater)and formalin-fixed
} material with equal efficiency.
}
} Finally, we understand that the fluorescence is short-lived - any comments
} as to how long a time period would be helpful.
}
}
} Many thanks
}
} Keith Ryan
} Marine Biological Association
} & University of Plymouth
} UK
}
} PLymouth
}
}
}


From daemon Fri Sep 21 08:05:28 2001



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 21 Sep 2001 08:51:01 -0400
Subject: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi-

my experience is the same as jim's. i've got about 10Khours on an fei
emitter now...still works great at } 500KX. its "on" all week and "off"
most weekends.

b-


} X-Comment: UConnVM.UConn.Edu: Mail was sent by d40h44.public.uconn.edu
} Date: Thu, 20 Sep 2001 17:59:41 -0400
} To: Microscopy-at-sparc5.microscopy.com
} From: Jim Romanow {bsgphy3-at-uconnvm.uconn.edu}
} Subject: Thermal FE
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Fri Sep 21 08:49:40 2001



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Fri, 21 Sep 2001 15:41:32 +0200
Subject: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey,

Thanks for the fast reaction, *but* you have forwarded my direct email to you to
the listserver as well.

Do you really want to discuss this matter at the listserver?

Of course, I am willing to discuss the advantage/disadvantage of our cameras in
publicity, buy I fear this might be regarded as advertisement!?

Therefore, at first, I want to know the opinion of the list server community!
Nestor, maybe do you can comment it?

Best wishes,
Ingo


Sergey Ryazantsev wrote:

} Dear Ingo
}
} Yes, I did check your WEB-site in the beginning of my search a few month
} ago as well as many others. My initial criteria were the following:
}
} -35 mm port mount;
} -price;
} -ability to have demo for the camera;
} -ability to have reasonable service in time.
}
} You product was excluded from initial list for the following reasons:
}
} - your Biocam camera, which was possible candidate is bottom-mounted;
}
} - on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
} any information about your headquarter in US or contact phones, so I was
} not able to talk to your representative (I was using the telephone call as
} a first "try" of the customer service quality: if I was on hold for more
} than 5 min, I excluded the candidate from my list).
}
} -location of your head office in Germany makes me skeptical about customer
} service quality company could provide in US.
}
} I would like to mention that my criteria is subjective and based on my own
} way to make a business and spent my money. My decision is not related to
} the real quality of your products.
}
} I would mention also that a few companies refused to give me demo on their
} cameras (or delayed with answer) and were excluded from the list as well.
}
} Sergey.
}



From daemon Fri Sep 21 08:57:44 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 21 Sep 2001 14:52:55 +0100 (GMT Daylight Time)
Subject: Re: Enviornmental SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Only the FEI ESEM allows you to have water in the chamber.
If I had the money I would get the FEGESEM.

Re maintainance, we do not have any extra problems with our
XL30 ESEM as our wet bullet apertures are replaced at
service. Our contract gives us 4 services a year. It would
be useful to get comments from a user without a service
contract.

Dave



On Thu, 20 Sep
2001 10:37:47 -0500 "Quinn, Tim Lee" {tquinn-at-ku.edu} wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } To Subscribe/Unsubscribe -- Send
Email to ListServer-at-MSA.Microscopy.Com } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} } Dear Listservers,
} } I am gathering data on enviornmental SEMs. Hopefully
I'll be setting up a } research facility within the coming
months. }
} Does anyone have preference to systems? }
} What, if any differences are there for maintinence
compared to a standard } SEM?
} } COST? (ouch)
} } Thanks,
} } Tim Quinn
} University of Kansas } Natural History Museum &
} Biodiversity Research Center } 1345 Jayhawk Blvd 414A
} Lawrence, KS 66045 } 785-864-4556
} tquinn-at-ku.edu }

----------------------------------------
Patton, David Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Sep 21 10:10:31 2001



From: Michelle_M_Adams-at-Brown.EDU (Michelle Adams)
Date: Fri, 21 Sep 2001 11:01:08 -0400
Subject: Immungold question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in subscribing to the microscopy listserver.

I have a question about immunogold labeling.
I am interested in surface labeling receptors and examining patterns of
internalization.
Has anyone tried to label the surface receptor with a primary antibody in
the live cell, induce internalization, fix and embed the cells. Then thin
section the preparation and do postembedding with a secondary antibody
tagged with a gold particle? Do you know if the primary antibody will
survive the embedding process with low-termperature embedding with
lowicryl?
Thanks for your help.
Michelle Adams




From daemon Fri Sep 21 10:29:33 2001



From: Christopher Gilpin :      Christopher.Gilpin-at-utsouthwestern.edu
Date: Fri, 21 Sep 2001 10:22:54 -0500
Subject: Field Cancelling systems and Mu Metal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I hope to be installing a new TEM soon and unfortunately have some concerns about magnetic field interference with the column.

Being a new "immigrant" to the USA I am not up to speed with vendors on this side of the Atlantic.
I need to know who supplies field canceling systems in the USA - calls from vendors welcome. I would also be interested in colleagues experiences with individual systems. I may have to deal with particularly high field strengths in the vicinity of the microscope so would be interested in what is the maximum field strength that such systems can compensate.
Another alternative would be to look into Mu metal lining for the microscope room. Again info on vendors would be most useful as well as anyone's experience of using this method of shielding.

Many thanks


Chris



Christopher Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
Cell Biology Department
University of Texas Southwestern Medical Center at Dallas
Dallas, TX, 75390-9039
phone +1 214 648 2827
fax +1 214 648 6408
christopher.gilpin-at-utsouthwestern.edu



From daemon Fri Sep 21 10:57:47 2001



From: Glenn Fried :      gfried-at-uiuc.edu
Date: Fri, 21 Sep 2001 10:53:51 -0500
Subject: Fwd: Re: Gatan's new UltraScan 1000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On a related note we just purchased a camera from Ingo. We have been
extremely happy with the performance. The camera was installed about one
mouth after tvips received the purchase order and the instating went very
smoothly. If anyone has questions please contact me.

Glenn




} Sergey,
}
} Thanks for the fast reaction, *but* you have forwarded my direct email to
} you to
} the listserver as well.
}
} Do you really want to discuss this matter at the listserver?
}
} Of course, I am willing to discuss the advantage/disadvantage of our
} cameras in
} publicity, buy I fear this might be regarded as advertisement!?
}
} Therefore, at first, I want to know the opinion of the list server community!
} Nestor, maybe do you can comment it?
}
} Best wishes,
} Ingo
}
}
} Sergey Ryazantsev wrote:
}
} } Dear Ingo
} }
} } Yes, I did check your WEB-site in the beginning of my search a few month
} } ago as well as many others. My initial criteria were the following:
} }
} } -35 mm port mount;
} } -price;
} } -ability to have demo for the camera;
} } -ability to have reasonable service in time.
} }
} } You product was excluded from initial list for the following reasons:
} }
} } - your Biocam camera, which was possible candidate is bottom-mounted;
} }
} } - on your WEB-site at http://www.tvips.com/Contact_en.htm I did not find
} } any information about your headquarter in US or contact phones, so I was
} } not able to talk to your representative (I was using the telephone call as
} } a first "try" of the customer service quality: if I was on hold for more
} } than 5 min, I excluded the candidate from my list).
} }
} } -location of your head office in Germany makes me skeptical about customer
} } service quality company could provide in US.
} }
} } I would like to mention that my criteria is subjective and based on my own
} } way to make a business and spent my money. My decision is not related to
} } the real quality of your products.
} }
} } I would mention also that a few companies refused to give me demo on their
} } cameras (or delayed with answer) and were excluded from the list as well.
} }
} } Sergey.
} }

Glenn Fried
Imaging Technology Group
Beckman Institute University of Illinois
phone 217 333 5493
Fax 217 244 6219



From daemon Fri Sep 21 12:56:44 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 21 Sep 2001 12:41:34 -0500
Subject: RE: Thermal FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I shut extraction voltage off only when
I was going on a long vacation and when I was told
there could be a possibility of power outage (I put a
microscope in a stand-by mode). Thermo cycling is the
second worst thing you can do to the tip after
power outage.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Nicol Aitken [mailto:nicol-at-semiconductor.com]
} Sent: Thursday, September 20, 2001 3:09 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Thermal FE
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} Hi Listers,
} I have a question about a schottky tipped FE Microscope.
} Specifically, what
} is the best way to shut the beam off at the end of the day.
} We will be using
} probably everyday for long sessions, so would beam blanking
} be preferable to
} shutting off the extraction voltage. I am wondering about the
} effects of tip
} life, beam stability, and contamination. Any advice or past
} experience would
} be greatly appreciated.
} Thanks again
} Nick Aitken
}
} Nicol Aitken
} Sample Preparation and Imaging Specialist
} Research and Development
} Semiconductor Insights Inc.
} email:nicol-at-semiconductor.com
} (613)599-6500 ext.4300
}
}
}


From daemon Fri Sep 21 16:12:35 2001



From: Brian J Laughlin :      brjlau18-at-US.ibm.com
Date: Fri, 21 Sep 2001 17:02:33 -0400
Subject: Cron deamon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been getting mail from root-at-mail.advancelink.com all afternoon.
Twenty or more of these emails. Other (I think that are on this list) have
been getting the same email but from my email address. I have not sent
these messages.

I have this bad feeling that this is a virus and it I have gotten from the
listserver somehow. Could the admin of this listserver check this out.

I am sorry if I am making false accusations.

Sincerely,

Brian Laughlin

FIB/TEM Engineer
IBM, Burlington, VT
Microelectronics Division
Surface and Materials Science Laboratory (Dept. GP8)


Lab: Bldg. 967-1 N18, (802) 769-1596
Fax: (802) 769-1220
Mail: IBM Burlington, 1000 River St., Essex Junction, VT 05452 Mailstop
967L




From daemon Fri Sep 21 17:34:36 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 21 Sep 2001 15:18:35 -0700
Subject: Re: Thermal FE discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What's the rationale for turning it off over weekends?

My system uses, I believe, a Denka ZrO/W filament in my
Amray 305FE gun assembly. I never shut the gun off.
The main problem with this particular SEM is that the
gun valve is manual. If the gun is on and the gun valve
is closed, there is most likely to be an air burp into the
gun chamber and will poison the filament. This process
also typically will burn the valves O-rings due to the high
intensity FE emitter beam. So the valve is never closed
until after turning off the filament.

The only reason I have found to worry about closing the
gun valve is if there is a power failure. That would shut
down the filament and all pumps (mech, turbo, column and
gun ion pumps). I solved this by using two Toshiba 1400XL+
6KVA dual conversion UPS units. Now, I shut the console
off but keep it leaves the pumps and gun running. Plus, the
UPS units

I log gun and column vacuum every few days along with
extractor current. Then, about every month, I measure
gun brightness. Running the way I am now, brightness
is +- no more than about 0.4nA. Iext may change 3-6uA
but brightness is way less affected by changes in Iext.
When Iext and brightness start to drop or waver, that is
an onerous sign that the gun is starting to fail.

I would tend to stick with this pattern since I know that
bringing up a new gun takes about a week for the
Iext and brightness to stabilize. I suspect that there would
be some proportional time to stabilize if shut down over
a weekend or for some extended time. Since I don't use
the SEM every day, if shutting off the filament during some
periods of time would extend its life, that is very desirable.

I have 9,020 hours on this gun since newly installed.
It is very stable.

gary g.

At 05:51 AM 9/21/2001, you wrote:

} hi-
}
} my experience is the same as jim's. i've got about 10Khours on an fei
} emitter now...still works great at } 500KX. its "on" all week and "off"
} most weekends.
}
} b-
}
}
} } X-Comment: UConnVM.UConn.Edu: Mail was sent by d40h44.public.uconn.edu
} } Date: Thu, 20 Sep 2001 17:59:41 -0400
} } To: Microscopy-at-sparc5.microscopy.com
} } From: Jim Romanow {bsgphy3-at-uconnvm.uconn.edu}
} } Subject: Thermal FE
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Nicol,
} }
} } For the past 4 years we have run the thermal emitter in our Zeiss LEO
} } DSM982 almost everyday Monday-Friday and off on weekends and vacations. The
} } previous emitter (Zeiss OEM) lasted 14,900 hours over 3 years. The present
} } emitter (from FEI) has 3,000 hours since November 2000. We routinely image
} } at } 100kx with great results.
} } What microscope do you have?
} }
} } Jim
} }
} }
} }
} }
} }
} }
} }
} } Nicol Aitken wrote:
} }
} } Hi Listers,
} } I have a question about a schottky tipped FE Microscope. Specifically, what
} } is the best way to shut the beam off at the end of the day. We will be using
} } probably everyday for long sessions, so would beam blanking be preferable to
} } shutting off the extraction voltage. I am wondering about the effects of tip
} } life, beam stability, and contamination. Any advice or past experience would
} } be greatly appreciated.
} } Thanks again
} } Nick Aitken
} }
} } Nicol Aitken
} } Sample Preparation and Imaging Specialist
} } Research and Development
} } Semiconductor Insights Inc.
} } email:nicol-at-semiconductor.com
} } (613)599-6500 ext.4300
} }
} } James S. Romanow
} } The University of Connecticut
} } Physiology and Neurobiology Department
} } Electron Microscopy Facility
} } Unit-2131
} } Storrs, CT 06269-2131
} } bsgphy3-at-uconnvm.uconn.edu
} } 860 486-2914 voice
} } 860 486-1936 fax
} }
} }
} }
} }
} }
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875



From daemon Fri Sep 21 18:29:07 2001



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 21 Sep 2001 17:21:57 -0600
Subject: Re: Random mail from your server to me

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this reply from a person about the cron daemon problem this
afternoon. If anyone has continuing problems, I suggest you contact them
directly.

--John
chandler-at-colostate.edu
Colorado State University



} From: "Kim M. Shiu" {kmshiu-at-mail.advancelink.com}
} Subject: Re: Random mail from your server to me
} To: chandler-at-lamar.colostate.edu (John Chandler)
} Date: Fri, 21 Sep 101 16:23:52 -0700 (PDT)
} MIME-Version: 1.0
} Status: RO
}
} Hi,
}
} There was a problem with a program on our server. It was not intentional
} to send multiple copies or spam anyone. The problem has been corrected
} and we apologize for any inconvenience it caused.
}
} Thanks.
}
} Kim Shiu
} Advancelink
}
} }
} } I sent this message to postmaster-at-advancelink.com and the messages continue
} } to be sent. Please attend to this immediately.
} }
} } --John
} }
} } FYI, I have receive 7 messages from your mail server this afternoon similar
} } or identical to the one below. This might indicate a situation that
} } requires your attention.
} }
} } John Chandler
} } Colorado State University
} } chandler-at-lamar.colostate.edu
} }
} }
} }
} } ============================================================================== }
} } }
} } Received: from mail.advancelink.com ([207.214.173.11]) by
} } lamar.ColoState.EDU (AIX4.3/8.9.3/8.8.8) with ESMTP id NAA237230 for
} } {john.chandler-at-colostate.edu} ; Fri, 21 Sep 2001 13:28:56 -0600
} } Received: (from root-at-localhost)
} } by mail.advancelink.com (8.8.5/8.8.5) id LAA29356;
} } Fri, 21 Sep 2001 11:35:00 -0700 (PDT)
} } Date: Fri, 21 Sep 2001 11:35:00 -0700 (PDT)
} } Message-Id: {200109211835.LAA29356-at-mail.advancelink.com}
} } From: root-at-mail.advancelink.com (Cron Daemon)
} } To: coccust-at-mail.advancelink.com
} } Subject: Cron {coccust-at-mail} csh -x ~/bin/get-dist } get-dist.log
} } X-Cron-Env: {SHELL=/bin/sh}
} } X-Cron-Env: {HOME=/usr/home/coc/coccust}
} } X-Cron-Env: {LOGNAME=coccust}
} } X-Cron-Env: {USER=coccust}
} } Status:
} }
} } cd /usr/home/coc/coccust
} } grep [a-z] /usr/home/coc/cocinfo/MAILING-LISTS/CURRENT-LIST.txt
} } chown coccust .forward
} } chown: Command not found.
} } chown coc .forward
} } chown: Command not found.
} } chmod 664 .forward
} } exit
} }
} }
} }
}




From daemon Sat Sep 22 11:06:21 2001



From: Karl Garsha :      keg-at-csd.uwm.edu
Date: Sat, 22 Sep 2001 11:03:05 -0500
Subject: Re: Immunogold / using mouse antibodies on mouse tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Stefan,
My initial reaction is that it is not common to use murine (mouse)
monoclonal antibodies on mouse tissue. The reason for this is that if these
murine antibody hybridomas were derived from mouse B-cells, and these
B-cells produced antibodies to a mouse protein, one would expect an
autoimmune response in the original mouse. In other words, it not be
logical to produce an antibody against mouse proteins in a mouse because
either: 1.) a secondary immune response would not be observed, or 2.) the
ensuing autoimmune response would kill or at least severly debilitate the
mouse. I guess recombinant monoclonal murine antibodies against mouse
proteins could be produced however. A more likely scenario is that these
mouse monoclonal antibodies are against a protein antigen derived from
another species.
A couple of important controls are in order. The simplest is to assess
non-specific binding of your secondary (gold-conjugate) antibody. To do
this you might block your tissue as usual, apply PBS or murine pre-immune
sera to the tissue in place of the primary antibody, and then apply your
secondary antibody.
Another important control is to asses non-specific binding of your
primary antibody. This is an isotype control. You should be able to find
out the isotype of your monoclonal antibody from the source (eg. IgG K2B).
Then obtain a monoclonal antibody of the same isotype which is not specific
for any mouse protein (eg. anti-dansyl or something). This is used in place
of the mouse specific monoclonal to assess non-specific binding due to
charge properties. An isotype control for the secondary antibody could be
conducted as well to assess the presence of tissue internal antibodies.
-Karl G.
_________________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
Room B650J
University of Illinois at Urbana-Champaign
Urbana, IL 61801
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Sat Sep 22 11:29:24 2001



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Sat, 22 Sep 2001 11:32:31 -0500
Subject: fouled objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,
As someone new to the challenge of managing multiuser equipment, I have
come to realize that it will be important to devise an effective strategy to
minimize wear and tear on instrumentation. It will also be important to
attempt to try to salvage the results of the inevetable mistakes that users
make.
I am hoping some of the seasoned veterans out there can offer some
advice. My question concerns how best to try to clean non-oil objectives
which have been used with oil. I was previously under the impression that
attempting this was futile, however I am hoping that I'm wrong. A collegue
of mine suggested sonicating in acetone for a couple of minutes, but I'm
hoping a gentler approach will suffice. Would the use of a surfectant or
detergent like NP-40 or tween or triton-X be a viable appoach? Are the
cements used in objectives commonly water soluble or are there safe organic
solvents to use to clean objectives?
I also have some nice oil-immersion objectives with dried films of oil
on them. Any suggestions?
If any one has some good suggestions for general references pertaining
to the cleaning and maintenence of objective lenses, I would welcome the
correspondence. I'm looking into the matter presently, but a point in the
right direction might expedite things.
Thank you.
-Karl Garsha
_________________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
Room B650J
University of Illinois at Urbana-Champaign
Urbana, IL 61801
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Sat Sep 22 12:27:23 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 22 Sep 2001 11:09:01 -0600
Subject: Cron deamon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

I have been getting these messages, too, but they seem to have stopped now.
I called advancelink, but only got a machine. My hunch is it is one of those
outfits that swamp your inbox with junk email. I simply told me email
application to just delete anything from advancelink.

As for a virus: It might be a virus on the advancelink servers, but the
messages I got contained some Unix commands (like "cron" and "grep"). To me
it looked more like some *really* clever person piped his Unix commands into
some form of mailing application.

As far as I could tell, none of the mails had any attachments, and I don't
think a mail application would run Unix commands embedded in an email.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Brian J Laughlin [mailto:brjlau18-at-US.ibm.com]
Sent: Friday, September 21, 2001 3:03 PM
To: Microscopy-at-sparc5.microscopy.com


I have been getting mail from root-at-mail.advancelink.com all afternoon.
Twenty or more of these emails. Other (I think that are on this list) have
been getting the same email but from my email address. I have not sent
these messages.

I have this bad feeling that this is a virus and it I have gotten from the
listserver somehow. Could the admin of this listserver check this out.

I am sorry if I am making false accusations.

Sincerely,

Brian Laughlin

FIB/TEM Engineer
IBM, Burlington, VT
Microelectronics Division
Surface and Materials Science Laboratory (Dept. GP8)


Lab: Bldg. 967-1 N18, (802) 769-1596
Fax: (802) 769-1220
Mail: IBM Burlington, 1000 River St., Essex Junction, VT 05452 Mailstop
967L




From daemon Sat Sep 22 13:33:29 2001



From: RCHIOVETTI-at-aol.com
Date: Sat, 22 Sep 2001 14:22:32 EDT
Subject: Re: fouled objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karl,

Whatever you do, please *don't* sonicate in acetone! This will dissolve the
adhesives that seal the lens and keep the lens elements in place, and it will
make the lens leaky. Acetone inside the lens will put you in deep trouble.

You can use anhydrous ether *very sparingly.* Use a cotton-tipped applicator
or a "Q-Tip" moistened with just a little ether and work quickly, gently
using circular motions on the front lens element. The ether will evaporate
rapidly, and you don't want to rub the lens with a dry cotton applicator, so
you will probably want to use several applicators. Ether is good for this
purpose because it evaporates so quickly. It's not around long enough to
damage the sealants and the adhesives.

You can follow this with a surfactant if necessary, but the ether should do
the trick. Some surfactants can leave a film on the surface of the lens
unless you work quickly and follow with a rinse of distilled water (using
cotton applicators, *not* a stream from a squeeze bottle). I would avoid the
use of surfactants if at all possible. If you do need some surfactant
action, first try a little commercial or consumer glass cleaner. Check the
labels for contents.

Remember, use ether sparingly, only enough to moisten the cotton applicator.
Avoid flooding the front lens element with ether (or alcohol, or anything,
for that matter).

Also, please note the usual cautions when working with ether (open flames,
sparks, electrical discharges, etc. should be avoided, use adequate
ventilation or work under a fume hood, etc.)

If you feel uncomfortable performing these steps, please call in a good
microscope service technician who knows what he/she is doing. I'm sure there
is one in your area. This can save you a lot of headaches (and a lot of
lenses).

Good luck!

Bob Chiovetti
GTI Microsystems
Leica Exclusive Regional Dealer
Desert Southwest (Arizona, New Mexico, West Texas USA)

***Disclaimer: These are my own opinions, gained from experience, not
necessarily those of GTI Microsystems or Leica Microsystems.***


In a message dated 09/22/2001 9:34:08 AM US Mountain Standard Time,
garsha-at-itg.uiuc.edu writes:

{ { Greetings Listers,
As someone new to the challenge of managing multiuser equipment, I have
come to realize that it will be important to devise an effective strategy to
minimize wear and tear on instrumentation. It will also be important to
attempt to try to salvage the results of the inevetable mistakes that users
make.
I am hoping some of the seasoned veterans out there can offer some
advice. My question concerns how best to try to clean non-oil objectives
which have been used with oil. I was previously under the impression that
attempting this was futile, however I am hoping that I'm wrong. A collegue
of mine suggested sonicating in acetone for a couple of minutes, but I'm
hoping a gentler approach will suffice. Would the use of a surfectant or
detergent like NP-40 or tween or triton-X be a viable appoach? Are the
cements used in objectives commonly water soluble or are there safe organic
solvents to use to clean objectives?
I also have some nice oil-immersion objectives with dried films of oil
on them. Any suggestions?
If any one has some good suggestions for general references pertaining
to the cleaning and maintenence of objective lenses, I would welcome the
correspondence. I'm looking into the matter presently, but a point in the
right direction might expedite things.
Thank you.
-Karl Garsha
_________________________________________________
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
Room B650J
University of Illinois at Urbana-Champaign
Urbana, IL 61801
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu
} }


From daemon Sat Sep 22 21:13:07 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Sat, 22 Sep 2001 19:04:54 -0700 (PDT)
Subject: Re: lanthanum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


JoAnn:
It has been a very long time, but I have used lanthanum quite extensively in
the past. And, yes, you do get a lot of precipitate (maybe flocculant is a
better word) in the various solutions. One of the things I did was to make
sure the washes were clean prior to starting the dehydration process. I
have not found that this flocculant material adversely affects the tissue
(or, for that matter, cultured cells). However, if there is a lot of
remnant material, it can/will appear as large clumps in the EM. If you are
going for cell surface labeling/coating, the large clumps can obscure any
fine details. As with a number of other non-specific labeling methods,
cleanliness, assuring adequate washing and attention to the minutiae will
give you good results. Best of luck.

Roger Moretz, Ph.D.
Dept. of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Mon, 17 Sep 2001 19:43:59 -0500, JoAnn Buchanan wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Hello folks. I am trying to fix and stain with lanthanum nitrate and
| have followed procedures in Hyatt's book. It mentions that lanthanum
| will precipitate in the cold, and when mixed with phosphate buffer.
| I have used cacodylate buffer, room temperature solutions and checked
| the pH of my buffer(7.2), but I am seeing a lot of ppt in all the
| solutions (glut fix, wash and osmium fix). I am wondering if anyone
| has any suggestions about how to alleviate this problem. Is the
| precipitate harmful to the tissue? Can I filter the solutions to get
| rid of big clumps? Is this normal? I would appreciate any feedback
| from someone who has used the stuff. Thanks, JoAnn
|
| JoAnn Buchanan
| Molecular and Cellular Physiology
| Stanford University School of Medicine
| Stanford, CA 94305
| 650-723-5856
|



Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
http://inbox.excite.com




From daemon Sun Sep 23 14:41:13 2001



From: wise :      wise-at-vaxa.cis.uwosh.edu
Date: Sun, 23 Sep 2001 14:29:46 -0500
Subject: Autoradiography of 14C?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

Does anyone know if it is possible to do TEM autoradiography of organic
compounds labelled with 14C?

Bob

Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
tele: (920) 424-3404
fax: (920) 424-1101
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html




From daemon Sun Sep 23 15:21:13 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Sun, 23 Sep 2001 15:00:16 -0500
Subject: Re: Autoradiography of 14C?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bob: There is a book called "Techniques of Autoradiography" by AW
Rogers (1979, Elsevier) that is a gold mine of info about LM & EM
autoradiography. He states that 14C quotes Salpeter & Salpeter, 1971
as showing a C14 specimen giving a HD (half diameter - distance of an
exposed silver grain from its source) resolution of 180 - 285 nm. of
course, a problem with lots of organic compounds is to fix them in
place during the various prep steps. good luck. tom

}
}
} Does anyone know if it is possible to do TEM autoradiography of organic
} compounds labelled with 14C?
}
} Bob
}
} Robert R. Wise, Ph.D.
} Associate Professor of Plant Physiology
} Department of Biology and Microbiology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} tele: (920) 424-3404
} fax: (920) 424-1101
} wise-at-uwosh.edu
} http://www.uwosh.edu/departments/biology/wise/wise.html

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Sun Sep 23 21:31:11 2001



From: aziz :      aaitouch-at-stevens-tech.edu
Date: Sun, 23 Sep 2001 22:31:46 -0700
Subject: postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Post-Doctoral Research Position

Energy-Loss Spectroscopy of Polymers and Biomaterials
at Stevens Institute of Technology

There is a post-doctoral opportunity within the Department of Chemical,
Biochemical, and Materials Engineering at the Stevens Institute of
Technology to develop and apply techniques associated with
spatially-resolved electron energy-loss spectroscopy. Application will
be made to study the morphology in biological tissue, in synthetic
polymers, and in mixtures of these two. Much of this work will be done
in collaboration with Unilever Research to pursue ongoing projects of
mutual interest.

The ideal candidate will have experience in electron optics and electron

energy-loss spectroscopy and preferably in techniques of ultramicrotomy
and cryo-ultramicrotomy.

The position can be filled as early as November, 2001. The appointment
will be for one year with a renewal dependent upon performance and
availability of funds. A multiyear appointment is anticipated.

The Stevens Institute of Technology is a small private university
concentrated on engineering, science, and technology. The Stevens
electron-optics laboratory contains a Philips CM20 FEG TEM/STEM, a
Philips CM30 SuperTwin TEM, and a Leo 982 FEG SEM. The CM20 FEG TEM/STEM

is equipped with a Gatan Enfina ccd PEELS system and a Gatan Multiscan
digital camera. Both are interfaced to an Emispec Vision acquisition
and control system. The facility is fully equipped with cryomicrotomy
and cryo-transfer capabilities to deal with frozen hydrated materials.

For further information please contact:

Professor Matthew R. Libera
Dept. Chemical, Biochemical, and Materials Engineering
Stevens Institute of Technology
Hoboken, New Jersey 07030
ph: 201-216-5259
fax: 201-216-8306
mlibera-at-stevens-tech.edu








From daemon Mon Sep 24 01:57:54 2001



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Mon, 24 Sep 2001 08:48:38 +0200
Subject: Si(Li) surface and FE-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all

What about the surface of the Si(Li) detector to be installed on a FE-SEM
?

I was adviced to take a 30 square mm insted of the classical 10 square
mm, to be installed on a cold FE-SEM. But if I look on my common
analytical conditions on a classical W filament SEM, I work with current
of 0.4 to 1 nA (mesured with a Faraday cup), with suffisant counting
rates (1500 to 2500c/s, with 12 microsecond tc). All FE-SEM can give such
current. Is it really necessary to take a detector with more surface (and
probably less resolution) ?

I am interrested in any advice from users.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Mon Sep 24 07:14:54 2001



From: ae3-at-njit.edu ()
Date: Mon, 24 Sep 2001 07:07:40 -0500
Subject: Ask-A-Microscopist: nanoparticles on a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ae3-at-njit.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
September 24, 2001 at 01:42:00
---------------------------------------------------------------------------

Email: ae3-at-njit.edu
Name: Alexandre Ermoline

Organization: New Jersey Institute of Technology

Education: Graduate College

Location: Newark, NJ, USA

Question: I'd like to get nanoparticles on a grid to study them by TEM.
I chose to use copper grid with plain carbon support film. But I
doubt if the contrast introduced by the support may be negligible
compared to that of the specimen because the thickness of the support
film is 20-30 nm and the nanoparticles sizes are in the range 10-50
nm.
Thank you for your kind attention
Alex

---------------------------------------------------------------------------


From daemon Mon Sep 24 07:18:43 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 24 Sep 2001 08:12:34 -0400
Subject: RE: Autoradiography of 14C?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Bob,
Two suggestions beyond Tom's good one:
1. "Thymidine Metabolism and Cell Kinetics", Elsevier,
1967.
2. "Autoradiography", Elsevier, Audrey Glauert's series in
EM, softcovers.
14C and 3H have been used together for double autoradiography,
because the penetrance of the beta particles from 14C is ~10X that of 3H,
although both tend to average out at lower levels in practical situations.
Some science on the subject:
http://laxmi.nuc.ucla.edu:8248/M248_98/autorad/lm_interp.html. The problem
with 14C IS the greater mean range and greater mean energy of the beta
particles. This is one reason that when there is a choice, 3H label is
preferred.
Working out the bugs for autoradiography can take a long time and
requires patience and attention to detail. I would recommend consideration
of the following: Mol Cell Biochem 1995 Nov 22;152(2):167-73, "Hyperplasia
in the rabbit bladder urothelium following partial outlet obstruction.
Autoradiographic evidence," Monson FC, Sun L, Wein AJ, Levin RM.; for a LM
method by which procedures for EM development might be expedited. If
you are interested and would like more information, please ask.

Also, good luck,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/




} ----------
} From: wise
} Sent: Sunday, September 23, 2001 3:29 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Autoradiography of 14C?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all,
}
} Does anyone know if it is possible to do TEM autoradiography of
} organic
} compounds labelled with 14C?
}
} Bob
}
} Robert R. Wise, Ph.D.
} Associate Professor of Plant Physiology
} Department of Biology and Microbiology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} tele: (920) 424-3404
} fax: (920) 424-1101
} wise-at-uwosh.edu
} http://www.uwosh.edu/departments/biology/wise/wise.html
}
}
}
}


From daemon Mon Sep 24 08:18:07 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 24 Sep 2001 09:09:23 -0400
Subject: RE: fouled objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Karl,
My opinion only!
Since many/most of our best objectives/oculars are coated for color
correction, Please don't use acetone on an objective - EVER!!! Please don't
use 100% ethanol, xylene or toluene on an objective - ever!? You may
concoct your own cleaner with non-ionic detergent (25%) and small amounts of
alcohol(s) and (ONLY IF REQUIRED) small - added when required - amounts of
xylene or toluene. I have known optical technicians who would rather scrape
a lens with the edge of a new scalpel blade or a fragment/piece of
double-edged razor blade than expose a lens to any solvent for more than a
brief moment for final - residual oil - removal. There are some who never
"rub". They "blow" with compressed air!
I have in my possession a product called "ROR" (URL:
http://www.ror.net/ [NO RELATION TO COMPANY]) which smells like a regular
glass cleaner, but has received "KUDOS" from photographic folks for its
effect on residual oils on lenses. Thus, it appears that this is a product
that does not remove color corrective coatings from photographic lenses.
I have also had in my possession for many years a product I acquired
from Xerox called: "Lens and Platen Cleaner", Reorder No. 8R1025 (6
bottles) and "Cleaning Absorbent" [really clean cotton], Reorder No. 8R25.
I have used this for residual oil removal for much longer than ROR, and I
have never been disappointed, though, of course, the Xerox cleaner was
intended for the windows and optics in their copiers.
The biggest problems in cleaning microscope optics are these:
1. corrective coatings which wear the more they are
cleaned,
2. mixing oils from different manufacturers (mentioned in a
recent email to the listserver), because they can react badly with one
another and harden,
3. front surface mirrors which are usually inside
fluorescent cubes or other complicated optical devices (these should be
cleaned only by someone who can afford to replace them),
4. permitting recidivist users to continue using better
microscopes, and
5. assuming that a new faculty member or post doc has been
properly taught or has properly learned while a graduate student. (I will
never forget the senior graduate student who removed an oil immersion lens
on a brand new microscope and poured it full of oil (from the nearby pint
bottle) as he followed the printed directions for its use!) I have NEVER
kept a pint bottle near a microscope since!

To your specific, other, and possibly implied questions (more than
you ever hoped for in one man's opinion):
1. Yes, you can clean the 'dry' objectives of caked on oil
with fluids. These are easier than many older oil immersion objectives,
because their exposed lens surfaces are usually flat.
2. Cleaning is preferable to discarding without trying.
3. Serious caked on oil can be scraped off (GENTLY!!!)
before trying to finish cleaning with a solution.
4. No, please do not soak the lens in anything.
5. Please, do not subject a complicated multi-element lens
system to immersion and ultrasound. You will NOT 'see the baby' by doing
this. The ultrasound will actually tend to separate cemented lens
combinations and to dissolve or fragment the cement that seals the face
element to the surrounding metal jacket. NO, please, definitely NO
ultrasound for microscope objectives, oculars, mirrors, filters, or prisms.
I HAVE used ultrasound to help clean grease from microscope focusing
mechanisms and from sticky leaf diaphragms, but after you dismantle your
first leaf diaphragm, you will know what real trouble is.
One way to help users is to predefine iris and field diaphragm
adjustments for each objective and mark the positions of those adjustments
so that indices are easily repeated. This is far more important for the
iris diaphragm than the field, because most unschooled microscopists learn
in their first class to use the iris diaphragm for contrast enhancement
(a.k.a., resolution diminution!). By placing the settings in plain view you
will give yourself the opportunity to review the purposes of the two
diaphragms with each new user and prevent unnecessary exposure of microscope
internals.
Is it obvious that I have a fixation on these matters?

} ----------
} From: Karl Garsha
} Sent: Saturday, September 22, 2001 12:32 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: fouled objectives
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings Listers,
} As someone new to the challenge of managing multiuser equipment, I
} have
} come to realize that it will be important to devise an effective strategy
} to
} minimize wear and tear on instrumentation. It will also be important to
} attempt to try to salvage the results of the inevetable mistakes that
} users
} make.
} I am hoping some of the seasoned veterans out there can offer some
} advice. My question concerns how best to try to clean non-oil objectives
} which have been used with oil. I was previously under the impression that
} attempting this was futile, however I am hoping that I'm wrong. A
} collegue
} of mine suggested sonicating in acetone for a couple of minutes, but I'm
} hoping a gentler approach will suffice. Would the use of a surfectant or
} detergent like NP-40 or tween or triton-X be a viable appoach? Are the
} cements used in objectives commonly water soluble or are there safe
} organic
} solvents to use to clean objectives?
} I also have some nice oil-immersion objectives with dried films of oil
} on them. Any suggestions?
} If any one has some good suggestions for general references pertaining
} to the cleaning and maintenence of objective lenses, I would welcome the
} correspondence. I'm looking into the matter presently, but a point in the
} right direction might expedite things.
} Thank you.
} -Karl Garsha
} _________________________________________________
} Karl Garsha
} Light Microscopy Specialist
} Imaging Technology Group
} Beckman Institute for Advanced Science and Technology
} Room B650J
} University of Illinois at Urbana-Champaign
} Urbana, IL 61801
} Tel: (217) 244-6292
} Fax: (217) 244-6219
} www.itg.uiuc.edu
}
}
}


From daemon Mon Sep 24 09:23:23 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Mon, 24 Sep 2001 10:22:03 -0400
Subject: NESM's October Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The October meeting of NESM (New England Society for Microscopy) will
be held Tuesday, October 16, 2001 at JEOL USA, Inc. in Peabody, MA.

This meeting will take an unusual format. It will be dedicated to a
single subject, electron back-scatter diffraction pattern analysis in
the SEM and will rely entirely on our sustaining members for content.
EBSD is a relatively new electron beam technique which allows the
determination of the crystallography of solid samples on a
microscopic scale. It can be applied to phase determination in
multi-phase samples, or orientation determination in single-phase
polycrystalline samples, the latter being especially useful if some
form of texture is present, as in drawn wires or thin films, for
example.

Four vendors of commercial EBSD systems will be part of our symposium
exploring the technique. The first part of the meeting will have
demonstrations of EBSD Systems by John Sutliff of HKL Technology,
Inc. and Neil Rowlands and Richard McLaughlin of Oxfor Instruments
Microanalysis Division.

The second half of the evening will have technical presentations on
the Theory and Applications of EBSD by Stuart Wright of TSL/EDAX and
Patrick Camus of Thermo-Noran, Inc.

There will be a buffet supper, courtesy of JEOL.

Members are invited to bring posters on the topic of EBSD analysis
for display at the meeting. Please call Tony Garratt-Reed
(617-253-4622) for more information if you are interested.

The pre-registration deadline is Friday, October 12th. Please
contact Mary McCann at (617-484-7865) or by email: mccanns-at-tiac.net.

I refer you to NESM's website for more information (times,
directions, registration fees, etc.) listed in the current newsletter
(September 2001).

Peggy Sherwood, Corresponding Secretary
NESM
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Mon Sep 24 10:50:45 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 24 Sep 2001 10:17:04 -0500
Subject: Re: Si(Li) surface and FE-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do much EDS with a tungsten filament. My experience is that my image
resolution suffers when I try to collect images at the same conditions used
for EDS. I think I am also normally in the 1 nA range.

Therefore, I would probably welcome the improved count rate/beam current
ratio that would be possible with a 30 mm2 detector. I could probably
accept a small decrease in peak resolution if I could more closely match
the conditions for imaging and x-ray analysis. Of course, I would not want
to pay too much for the improvement. How much more does a 30mm2 cost
compared to a 10 mm2 detector?

Warren

At 08:48 AM 9/24/2001 +0200, you wrote:

} Hello all
}
} What about the surface of the Si(Li) detector to be installed on a FE-SEM
} ?
}
} I was adviced to take a 30 square mm insted of the classical 10 square
} mm, to be installed on a cold FE-SEM. But if I look on my common
} analytical conditions on a classical W filament SEM, I work with current
} of 0.4 to 1 nA (mesured with a Faraday cup), with suffisant counting
} rates (1500 to 2500c/s, with 12 microsecond tc). All FE-SEM can give such
} current. Is it really necessary to take a detector with more surface (and
} probably less resolution) ?
}
} I am interrested in any advice from users.
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Mon Sep 24 13:24:47 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 24 Sep 2001 14:07:49 -0400
Subject: Re: fouled objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karl,
I manage a multi-user optical microscopy facility. Brace yourself.
You will have to resign yourself to the fact that no matter how
carefully you explain things to your users, someone is going to drag
a dry lens through oil, get fingerprints on the ocular lenses, and do
things you hadn't imagined. I try (not always successfully) to keep
a schedule of visually inspecting the lenses, etc and correcting the
damage before it gets too far gone. both of our microscopes are
inverted, so I have had to devise little "diapers" for the lenses to
absorb the excessive amount of immersion oil my users feel they need,
so that it doesn't drip down and work its way into the lenses or even
the body of the 'scope itself! (yes, they are that sloppy).
We routinely use a solution of Windex (the standard blue stuff)&
water (1:1) to keep our lenses clean. It works very well on oil that
is still liquid. For the encrusted lenses, you may need something
stronger. I would call your sales/service rep for the microscope and
ask what they recommend for their lenses, since the coating on the
lenses may be damaged by different solvents.
Vigilance pays. Good luck.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Sep 24 13:24:48 2001



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu
Date: Mon, 24 Sep 2001 13:17:52 -0500
Subject: anti-GFP antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listmembers,

Does anyone know of a commercially available anti-GFP antibody conjugated to
gold? (preferably 10 nm). I know we can use a 'bridging' step to get to the
gold, but I wanted to know if there is a direct secondary available. Thanks
in advance for your help.

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Mon Sep 24 14:01:12 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 24 Sep 2001 08:54:59 -1000 (HST)
Subject: Re: Ask-A-Microscopist: nanoparticles on a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Alex-

} Question: I'd like to get nanoparticles on a grid to study them by TEM.
} I chose to use copper grid with plain carbon support film. But I
} doubt if the contrast introduced by the support may be negligible
} compared to that of the specimen because the thickness of the support
} film is 20-30 nm and the nanoparticles sizes are in the range 10-50
} nm.

I am imaging Si nanoparticles of as small as 4 nm on carbon support
film. There is really very little contrast between the Si and C, so I hope
you're looking at something a bit more electron dense! I make my own
carbon films, and I have no idea how thick they are. I'm using a LEO 912
EFTEM at 100kV, and I can easily see particles of 10 nm and, with a lot of
fiddling around, down to just about 4 nm. Smaller than that and I am
seeing what may be evaporated carbon particles.

I think the main trick is to have as thin a support film as you can get
- I make my own with variable results, and Ted Pella has some ultrathin
carbon on lacy Formvar, I think. Make sure your scope is well
aligned. Use a high enough accelerating voltage that you can resolve the
things, but not so high that you shoot right through them and don't get
enough electrons scattered away to see them. And I hope you're using a
heavier element than I am!

Good luck.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Sep 24 15:54:24 2001



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 24 Sep 2001 16:35:47 -0500
Subject: Re: SEM imaging on CNT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We typically examine SWCNT's by ultrasonically dispersing a small
quantity in a 10 ml beaker of ethanol, remove the beaker from the
ultrasonic bath, dip a holey carbon film supported on a copper grid
into the solution to pick up some of the nanotubes, and then place
the grid on bibulous paper to wick away the excess solvent. This
sample can then be used for both TEM as well as SEM.

I would be careful not to put a large quantity of loose SWCNT's into
your SEM because typically the commercially available carbon
nanotubes are produced with magnetic catalysts (iron). The magnetic
field of the final lens can pull them off your mount and into your
lens! We had a researcher looking at nanotubes grown on a clump
steel wool, part of the sample was pulled into the objective lens of
our FE-SEM, we noticed a glow coming from the final lens with our
chamber view camera when the infrared diodes were turned off! An
expensive light bulb! The service engineer had to clean the final
lens.



At 7:55 AM -0500 9/17/2001, Haeseong Lee wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} ??
} Hello.
}
} Could anybody recommend the good method of sample preparation for
} SEM imaging on carbon nano tubes?
} The sample is a powder type including single wall carbon nanotubes (SWCNTs).
} Thank you very much in advance.
}
} Haeseong Lee
}
} {mailto:haeseong-at-hanyang.ac.kr} haeseong-at-hanyang.ac.kr


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Mon Sep 24 16:00:07 2001



From: Mark Wall :      wall1-at-llnl.gov
Date: Mon, 24 Sep 2001 13:50:18 -0700
Subject: EDS procedures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of any documented EDS calibration (energy display,
quantification, etc.) procedures that have been certified in any way?
i.e. NIST, ISO-9000 or any other.

thanks,

Mark Wall
LLNL
925 423 7162


From daemon Mon Sep 24 16:02:57 2001



From: Krueger, Eugene W. :      krueger.eugene-at-mayo.edu
Date: Mon, 24 Sep 2001 15:53:24 -0500
Subject: 3D reconstruction from serial sections, 2nd request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to all in the microscopy community,

I have previously posted a request for information on 3D reconstruction from
serial sections.....but I only recieved replies from vendors and people
wanting a summary of the replies.

{ { {I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you choose.
Also vendors feel free to send me any info you might have on the products
you sell.} } }

I would really like to hear from the users of these programs! I would be
glad to submit a summary to all interested parties (yes I still have your
addresses).

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/


From daemon Mon Sep 24 20:47:29 2001



From: Moran Scientific :      kmoran-at-goulburn.net.au
Date: Tue, 25 Sep 2001 11:31:06 +1000
Subject: EDS & XRM Courses - NSW, Australia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Moran Scientific is running EDS and XRM Microanalysis Courses from Monday
15th October to Friday 19th October at the Old Bungonia School. These are
Courses designed for Beginners and those more Advanced. This is our last
call for any people interested in attending.
Please email us at the earliest opportunity for more information.
Kind Regards,
Diana Moran
Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"



From daemon Mon Sep 24 23:35:05 2001



From: Divakar :      divakar-at-igcar.ernet.in
Date: Tue, 25 Sep 2001 09:54:35 +0530
Subject: RE: Ask-A-Microscopist: nanoparticles on a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alex:

Contrast depends on the TEM technique that you plan to use. If you need
particle size and morphology, diffraction contrast (bright / dark field
imaging) may be acceptable on the carbon support film. If it is lattice
structure by phase contrast microscopy, holey carbon film coated grids
may be a better option. In the latter case, Jose Yacaman, Materials
Trans JIM, 40 (1999), p 141 - 145 may be of interest to you.

Best wishes.

On Monday, September 24, 2001 5:38 PM, ae3-at-njit.edu [SMTP:ae3-at-njit.edu]
wrote:
}
----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------
} -.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (ae3-at-njit.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
} September 24, 2001 at 01:42:00
}
----------------------------------------------------------------------
} -----
}
} Email: ae3-at-njit.edu
} Name: Alexandre Ermoline
}
} Organization: New Jersey Institute of Technology
}
} Education: Graduate College
}
} Location: Newark, NJ, USA
}
} Question: I'd like to get nanoparticles on a grid to study them by
} TEM.
} I chose to use copper grid with plain carbon support film. But I
} doubt if the contrast introduced by the support may be negligible
} compared to that of the specimen because the thickness of the support
}
} film is 20-30 nm and the nanoparticles sizes are in the range 10-50
} nm.
} Thank you for your kind attention
} Alex
}
}
----------------------------------------------------------------------
} -----




From daemon Tue Sep 25 03:30:10 2001



From: Jean Louis HEITZ :      jl.heitz-at-critt.fr
Date: Tue, 25 Sep 2001 10:20:28 +0200
Subject: Re: Si(Li) surface and FE-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


dear Jacques,

we currentlly use low currents (0.80 nA) for oxide glass EDX analysis
on a tungsten filament SEM. The count rate is approx. 2000 cps at 30%
dead time using a process time which correponds to 129 eV resolution
on Mn. the SiLi detector surface is 10 mm². For elements in the range
0.5 - 1% a higher current is necessary. For elements { 1% we use WDX
if the % has to be known precisely.

I think that a 30 mm² would be better if you need to analyse oxides or
organic compounds at very low SEM currents ( { 0.5 nA). (The distance
detector - sample has to be considered, too). It is more convenient to
use the same SEM currents for imaging and analysis.
A lower resolution is not necessairely a bad choice, provided you
record your own standards and use an FLS-deconvolution program.

kindly,

Jean Louis HEITZ

CRITT Matériaux-LNE Est
19 rue de Saint Junien, BP 23
F - 67305 SCHILTIGHEIM CEDEX
tel. +33(0) 388 191 510
fax +33(0) 388 191 514
email jl.heitz-at-critt.fr
web www.critt.fr



From daemon Tue Sep 25 07:33:47 2001



From: Rakeshnie Ramoutar :      PHYSIOL5-at-akad.sun.ac.za
Date: Tue, 25 Sep 2001 14:25:08 +0200
Subject: LM: fixing sugarcane tissue for mRNA in situ hybridization studies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there
Does anyone know or can suggest how I can fix and either wax or
resin embed sugarcane stem tissue for the purpose of in situ work.
Have tried a number of protocols but the end result is almost
always the same: tears in the sections, thus making it difficult to
continue with the rest of the hybridization procedure. Any help or
suggestion would suffice.
Thanx
Rakeshnie Ramoutar
Msc Student
Institute for Plant Biotechnology
University of Stellenbosch


From daemon Tue Sep 25 07:44:25 2001



From: Jean Louis HEITZ :      jl.heitz-at-critt.fr
Date: Tue, 25 Sep 2001 14:36:53 +0200
Subject: Re: EDS procedures / standardization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


the ISO TC (technical comitee) 202 works on microanalysis (SEM EDX/WDX
- EPMA).
you can contact your local standardization agency for information.

best regards,

Jean Louis HEITZ

CRITT Matériaux LNE Est
19, rue de Saint Junien - BP 23
F - 67305 SCHILTIGHEIM CEDEX
tel. +33(0) 388 191 510
fax +33(0) 388 191 514
email jl.heitz-at-critt.fr
web www.critt.fr



From daemon Tue Sep 25 08:20:43 2001



From: Matthias =?iso-8859-1?Q?M=F6rgelin?= :      Matthias.Morgelin-at-medkem.lu.se
Date: Tue, 25 Sep 2001 08:15:24 -0500
Subject: thin sectioning of cartilage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have the problem to visualize the cytoskeleton of cartilage cells
(chondrocytes) in articular cartilage of wild type and knockout mice.
They are suspected to show differences in cytoskeletal organization.
What is the most appropriate way to obtain highest possible contrast
for the cytoskeleton (tricks during fixation, embedding, poststaining
etc?)? With many thanks in advance for your help

Matthias Mörgelin, PhD
Dept of Cell and Moleculer Biology
Lund University
BMC, C12
S-221 84 Lund
Sweden

phone +46 2220741
fax +46 2113417
e-mail: matthias.morgelin-at-medkem.lu.se


From daemon Tue Sep 25 08:45:15 2001



From: Lauran Oomen :      oomen-at-nki.nl
Date: Tue, 25 Sep 2001 15:36:50 +0200
Subject: LM-FISH-SmartCapture2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Our multi-user facility is in the process of upgrading one of our
microscope (fully motorized Zeiss) / camera (Photometrics) systems and
we are also considering the purchase of the SmartCapture2 software from
Digital Scientific (UK).
However, there is no demo version of this software available and hence
the (dis)advantages of the software ar difficult to judge.
In order to be able to make a well founded decision it is of great
importance to us to get information from existing users.
We would be very glad if you could comment on the following, taking into
account that the software has to be used in a multi-user environment


* SmartCapture2 in general
* the ease of use (interface)
* the flexibility of the software
and more specifically
* the autofocus functioning
* the batch capture possibility
* any other aspect which you find important

I would greatly appreciate your comments, both positive and negative,
and I already thank you in advance for your time taken.
Those willing can of course also respond ofline.

Lauran Oomen
Manager Digital Microscopy Facility
The Netherlands Cancer Institute
Amsterdam
The Netherlands
tel. +31-205121889
oomen-at-nki.nl




From daemon Tue Sep 25 10:28:34 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Tue, 25 Sep 2001 08:18:27 -0700
Subject: RE: 3D reconstruction from serial sections, 2nd request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Eugene,
There is an NIH derivative that I've used for various stereology
applications called Object Image (http://simon.bio.uva.nl/object-image.html).
It also has a module for creating wire frame outlines of serial section type of
information. Once made, it even has the ability to export the wire frame data
to another program that can rotate the 3D object. I like to use this program to
collect statistical info on size and shapes of particles, and I've been pleased
with its useability, and easy learning curve. I haven't done any 3D work with
it, but I've played around with the included tutorial models - pretty fun. The
software is mac-based, has modest processing requirements, and is free. Its a
great software application to have in your "toolbox". Good luck.

-Brad

----------
From: Krueger, Eugene W.
Sent: Monday, September 24, 2001 1:53 PM
To: 'Microscopy-at-sparc5.microscopy.com'
Subject: 3D reconstruction from serial sections, 2nd request

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Hello to all in the microscopy community,

I have previously posted a request for information on 3D reconstruction
from
serial sections.....but I only recieved replies from vendors and people
wanting a summary of the replies.

{ { {I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples
opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you
choose.
Also vendors feel free to send me any info you might have on the
products
you sell.} } }

I would really like to hear from the users of these programs! I would
be
glad to submit a summary to all interested parties (yes I still have
your
addresses).

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/




From daemon Tue Sep 25 11:09:17 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 25 Sep 2001 11:03:09 -0500
Subject: Electron Microscopist Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



US-IL-Chicago Northwest-Electron Microscopist -- SEM


----------------------------------------------------------------------------
----

UOP LLC, located in northwest suburban Chicago, is an international leader
in technology, products and services for the petroleum processing and
separation industries. Our R & D Deptartment, which supports fundamental and
market-focused R & D, has an immediate need for an Electron Microscopist to
work with chemists and engineers throughout the company on structural and
analytical characterization issues relating to catalytic, adsorbent, ion
exchanger and metallurgy materials.

Technical assignments will include:

- Interaction and collaboration with solid state and inorganic chemists,
applications specialists, and technical service personnel in applying
appropriate microscopic and data analysis techniques to problem solving in
catalytic and adsorbent materials fields.
- Interaction and collaboration with existing microscopy group to
effectively utilize SEM and EDXS facilities, including training of junior
technical staff.
- Development and continuation of in-house expertise in current and emerging
scanning electron microscopy techniques and applications.

Minimum qualifications include:

- BS/MS with multi-year relevant work experience, or recent PhD in Geology,
Chemistry, Materials Science, or Physics (preferred coursework in electron
microscopy, solid state chemistry and physics).
- Expertise in SEM and electron probe X-ray microanalysis techniques.
- Knowledge of sample preparation techniques, including ultramicrotomy.
- Excellent team and communication skills.

Knowledge of other microscopic characterization techniques, for example TEM
and STEM, and industrial R&D experience desirable but not essential. Above
all, we are seeking a motivated individual, who will demonstrate a
willingness to learn and openness to acquiring new areas of expertise in
characterization, chemistry and materials properties.

We offer a state-of-the-art scientific research environment coupled with
exciting career opportunities coupled with a highly competitive compensation
and benefits package including medical and dental coverage, 401(k) Plan,
Pension Plan and tuition reimbursement.

US employment authorization required.

UOP is an Equal Opportunity Employer

Visit us at www.uop.com



----------------------------------------------------------------------------
----
Additional Information
Position Type: Full Time, Employee
Ref Code: MB-01-11

----------------------------------------------------------------------------
----
Contact Information
UOP Internet Resume Manager
cro-at-uop.com
UOP LLC
25 E. Algonquin Road, P.O. Box 5017
Des Plaines IL 60017-5017
Fax: 847.391-2921

----------------------------------------------------------------------------
----

To learn more about UOP: http://company.monster.com/llc


From daemon Tue Sep 25 11:24:43 2001



From: George Langford :      amenex-at-amenex.com
Date: Tue, 25 Sep 2001 12:19:40 -0400
Subject: Microscopy equipment listed on eBay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists !

Amenex is giving up its laboratory space, so much of our
equipment is for sale. Check out the eBay seller list
for amenex-at-amenex.com, which includes:

ETEC Autoprobe scanning electron microprobe
ETEC evaporative coater
Wilson Tukon microhardness tester
Bausch & Lomb Balphot metallograph
Lasico digital filar eyepiece
Philtec sectioner
Buehler dual-motor polishing table
Oscar Fisher print dryer

I can be reached at amenex-at-amenex.com or at (610) 430-0117
if you have any questions.

Best regards,
George Langford
amenex-at-amenex.com
http://www.amenex.com/


From daemon Tue Sep 25 12:42:18 2001



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Tue, 25 Sep 2001 10:34:50 -0700
Subject: Need Manual: AO Model 880 Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need the user's manual for an American Optical 880 series microtome.
These microtomes clamp to a bench. The specimen is fixed and the knife is
moved by a parallelogram mechanism.

Will glady defray photocopying and mailing expense.



From daemon Tue Sep 25 13:30:57 2001



From: Vachik Hacopian :      vhacopian-at-wellesley.edu
Date: Tue, 25 Sep 2001 14:24:01 -0400
Subject: water recirculators for electron microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to purchase a new water recirculator for our electron microscope
(Philips CM-10). Is there a consensus of opinion on what the best
recirculator is, reliability being the highest consideration? Thanks.

Vachik Hacopian




From daemon Tue Sep 25 13:31:40 2001



From: Scott J. Robinson :      sjrobin-at-itg.uiuc.edu
Date: Tue, 25 Sep 2001 13:35:12 -0500
Subject: SEM: LaB6 filament for ISI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Out There--

I have an unused LaB6 filament, made by FEI and dated 10-24-95, for an ISI
SEM. It's been stored in a desiccator since it was purchased. The 'scope it
was intended for is long gone. I will be pleased to send it to the first
person who might be able to use it. Please contact me offline.

Thanks

Scott Robinson

Microscopy Suite, room B650J
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana IL 61801
217 265-5071 (office); 217 244-6219 (fax)
sjrobin-at-uiuc.edu



From daemon Tue Sep 25 13:45:27 2001



From: Richard W. Fonda :      fonda-at-anvil.nrl.navy.mil
Date: Tue, 25 Sep 2001 14:39:03 -0400
Subject: Postdoc opening at NRL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL POSITION AT THE NAVAL RESEARCH LABORATORY

The U.S. Naval Research Laboratory has an opening for a postdoctoral
fellow with experience in transmission electron microscopy and a
strong background in physical metallurgy and/or materials science.
The successful candidate will study phase transformations and their
resultant microstructures in materials of interest to the U.S. Navy.
Current research efforts are focussed on steels, aluminum-based
alloys, magneto-electronic thin films, multilayers, and various
welding processes (friction stir, laser, gas metal arc). Selection
of a specific program will depend on the programs available and
interests of the candidate. This position will employ a variety of
analytical equipment as needed. Microscopy facilities available
include a Philips CM-30 TEM, a Hitachi H-9000 HRTEM, a Leo 1550 SEM
with OIM & EDS, and a new JEOL 2100-FEF energy filtered TEM (to be
installed next summer). Additional facilities include a FIB, a
quenching and deformation dilatometer, a Gleeble thermo-mechanical
simulator, a state-of-the-art isothermal heat treatment facility, and
the NRL visualization laboratory for three-dimensional analysis of
microstructures. If interested, please send resume and names of
references to Richard Fonda, Code 6324, Naval Research Laboratory,
Washington, DC 20375. If you have any questions regarding this
position, please contact either George Spanos (202-767-5799,
spanos-at-nrl.navy.mil) or Richard Fonda (202-767-2622,
fonda-at-nrl.navy.mil) for further details.
NOTE: this position is only open to US citizens or residents with a green card.
________________________________________________________________
Richard W. Fonda Naval Research Laboratory
(202) 767-2622 Code 6324
(202) 767-2623 fax Washington DC 20375
________________________________________________________________



From daemon Tue Sep 25 15:22:25 2001



From: Thearith H. Ung :      tung-at-qdots.com
Date: Tue, 25 Sep 2001 08:38:12 -0700
Subject: Re: Ask-A-Microscopist: nanoparticles on a grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Alex,

I have looked at particles down to 1.5 nm in diameter, using grids coated
with incredibly thin carbon film (1-2 nm thick). I get very high particle
contrast, and the particles I look at aren't very dense either. I purchase
these from the University of Chicago. The contact person is Dr. Robert
Joseph (BOB-at-befvax.uchicago.edu). I am sure he will be more than delighted
to sell you some.

Regards,
Thearith



-----Original Message-----
} From: Tina Carvalho [mailto:tina-at-pbrc.hawaii.edu]
Sent: Monday, September 24, 2001 11:55 AM
To: ae3-at-njit.edu
Cc: Microscopy-at-sparc5.microscopy.com


Hi, Alex-

} Question: I'd like to get nanoparticles on a grid to study them by TEM.
} I chose to use copper grid with plain carbon support film. But I
} doubt if the contrast introduced by the support may be negligible
} compared to that of the specimen because the thickness of the support
} film is 20-30 nm and the nanoparticles sizes are in the range 10-50
} nm.

I am imaging Si nanoparticles of as small as 4 nm on carbon support
film. There is really very little contrast between the Si and C, so I hope
you're looking at something a bit more electron dense! I make my own
carbon films, and I have no idea how thick they are. I'm using a LEO 912
EFTEM at 100kV, and I can easily see particles of 10 nm and, with a lot of
fiddling around, down to just about 4 nm. Smaller than that and I am
seeing what may be evaporated carbon particles.

I think the main trick is to have as thin a support film as you can get
- I make my own with variable results, and Ted Pella has some ultrathin
carbon on lacy Formvar, I think. Make sure your scope is well
aligned. Use a high enough accelerating voltage that you can resolve the
things, but not so high that you shoot right through them and don't get
enough electrons scattered away to see them. And I hope you're using a
heavier element than I am!

Good luck.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *

* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*

****************************************************************************



From daemon Tue Sep 25 15:47:05 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 25 Sep 2001 13:39:54 -0700
Subject: RE: 3D reconstruction from serial sections, 2nd request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugene,

our group is involved in developing a program for 3-d reconstruction of TEM
serial sections for modeling processes in prokaryotic cells. I asked the person
responsible for the images stacking part (not a microscopist), and will try to
translate his answer:
It depends on what someone would want to do with the reconstruction. If you
wanted to just look at the image of the reconstruction, then there is some
software that you can kind of do this (with a lot of fiddling of the stack of
data to get it into the program). The two packages are IBM's OpenDX and the VTK
toolkit.
If the purpose is to produce 3-D modeling geometry (like what we want to do),
then there is nothing available to his knowledge. He says this is partly because
there is no 3-D modeling code available that could consume such data.
I do know of two groups that are doing section-by-section segmenting of TEM
images (by hand) so that they can reconstruct a 3-D image. Please let me know if
you'd like more info.
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


Hello to all in the microscopy community,

I have previously posted a request for information on 3D reconstruction from
serial sections.....but I only recieved replies from vendors and people
wanting a summary of the replies.

{ { {I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you choose.
Also vendors feel free to send me any info you might have on the products
you sell.} } }

I would really like to hear from the users of these programs! I would be
glad to submit a summary to all interested parties (yes I still have your
addresses).

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/


From daemon Tue Sep 25 15:49:31 2001



From: Scott J. Robinson :      sjrobin-at-itg.uiuc.edu
Date: Tue, 25 Sep 2001 15:52:48 -0500
Subject: SEM filament out the door

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The LaB6 filament for the ISI is now spoken for.

Thanks

Scott


Microscopy Suite, room B650J
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana IL 61801
217 265-5071 (office); 217 244-6219 (fax)
sjrobin-at-uiuc.edu



From daemon Tue Sep 25 19:02:04 2001



From: Norman C Miller :      Norman_C_Miller-at-raytheon.com
Date: Tue, 25 Sep 2001 18:53:07 -0500
Subject: used Amray SEM and PGT EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

I emailed that we have an Amray 1000A SEM and a PGT EDS with Sun
workstation that we do not need. They are available to anyone who would
like to have them and will take care of taking them away. They are listed
on the MSA web site under surplus equipment.

N. Carl Miller


From daemon Tue Sep 25 19:02:04 2001



From: hwang-at-Guardian.com
Date: Tue, 25 Sep 2001 18:52:43 -0500
Subject: Help-refurbish an optical microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I have a Nikon Optiphot 88 microscope which doesn't give a good image. I
have had Nikon representative in and got little help to improve the
situation. So I am looking for an independent expert to check the
microscope out and repair it. In addition, I'd like to add some more
features such as dark field and possibly add couple of new lenses.

If someone know companies or individuals who provide that kind of service,
please let me know.

Thanks in advance.

Hong Wang
Principle Materials Scientist
Guardian Science and Technology Center
14511 Romine Road
Carleton, MI 48117


From daemon Wed Sep 26 01:34:31 2001



From: Angus Netting :      angus.netting-at-adelaide.edu.au
Date: Wed, 26 Sep 2001 15:56:44 +0930
Subject: Oxford CT1500HF cryostage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi folks,

We have an Oxford CT 1500HF Cro Transfer System on our SEM
and have blown the nude ion gauge. We have no information
on the part , description or Part no.

If any one has one of these units,
or can provide information from their manual.
I would be very grateful to hear from them.

Many thanks

--


Angus Netting
CEMMSA
(Centre for Electron Microscopy & MicroStructure Analysis)
Adelaide University
Ph: 61 8 8303 5855
Fax: 61 8 8303 4356
http://www.adelaide.edu.au/CEMMSA


From daemon Wed Sep 26 06:12:09 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 26 Sep 2001 12:04:21 +0100 (GMT Daylight Time)
Subject: HMDS Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use HMDS to "dry" SEM specimens. My bottle advises
"Moisture sensitive. Stored under argon" Up to now I have
used bottles stored in the fridge for up to a year.

1. Does anyone take any precautions to keep their HMDS
dry?

2. Furthermore how long would you regard an opened
bottle as useful?

3. We use molecular sieves to keep ethanol and acetone dry
- should we put it in HMDS?

Dave


----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Sep 26 08:49:31 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 26 Sep 2001 09:34:24 -0400
Subject: Re: water recirculators for electron microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} We need to purchase a new water recirculator for our electron microscope
} (Philips CM-10). Is there a consensus of opinion on what the best
} recirculator is, reliability being the highest consideration? Thanks.
}
} Vachik Hacopian
****************

I have a 21 year-old Haskris for my JEOL. It has needed very little
attention:, mostly water changes when the engineers do my PMs. I had
to replace the compressor 10 years ago, and just had a leak in the
refrigerant tubing repaired.

(No financial interest in Haskris or JEOL)

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Sep 26 09:12:41 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 26 Sep 2001 08:05:08 -0600
Subject: RE: 3D reconstruction from serial sections, 2nd request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have watched this thread and have refrained from posting since I did not
want to "advertise" our products, but I believe that there is some erroneous
information now.

Alice Dohnalkova said: "If the purpose is to produce 3-D modeling geometry
(like what we want to do),
then there is nothing available to his knowledge". Although I am not sure,
what "3-D modeling geometry" entails, there is software that takes images,
allows you to define certain features in each image of a stack (by hand or
semiautomatically), and take all that information from the different slices
and create a 3-D model of whatever was sliced. Of course you can then do
measurements on the reconstruction. There are several of those packages out
there (our 3D module is one of them).
The other type of software is "voxel" based. In essence a stack of images is
treated as a "cube" of information, which can then be sliced and lookt at in
different directions.

The first option is normally used to recreate a 3-D representation of the
object, which can then be further analyzed with regards to it's position
relative to other objects or for certain 3-D parameters (volume, surface, 3D
angles, etc.).

The second type (voxel-based) is normally used to create a 3-D information
cube of the entire stack, then take slices in different directions and
analyze these (artificial) images.

Both usually require that you know the separation between slices (from your
microtome settings), and that you can apply a number of operations before
creating the 3D shape to account for shift, rotation and distortion. It is
sometimes necessary to embed a "standard" in the sample to be able to do
that.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dohnalkova, Alice [mailto:Alice.Dohnalkova-at-pnl.gov]
Sent: Tuesday, September 25, 2001 2:40 PM
To: 'Krueger, Eugene W.'; 'Microscopy-at-sparc5.microscopy.com'


Eugene,

our group is involved in developing a program for 3-d reconstruction of TEM
serial sections for modeling processes in prokaryotic cells. I asked the
person
responsible for the images stacking part (not a microscopist), and will try
to
translate his answer:
It depends on what someone would want to do with the reconstruction. If you
wanted to just look at the image of the reconstruction, then there is some
software that you can kind of do this (with a lot of fiddling of the stack
of
data to get it into the program). The two packages are IBM's OpenDX and the
VTK
toolkit.
If the purpose is to produce 3-D modeling geometry (like what we want to
do),
then there is nothing available to his knowledge. He says this is partly
because
there is no 3-D modeling code available that could consume such data.
I do know of two groups that are doing section-by-section segmenting of TEM
images (by hand) so that they can reconstruct a 3-D image. Please let me
know if
you'd like more info.
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


Hello to all in the microscopy community,

I have previously posted a request for information on 3D reconstruction from
serial sections.....but I only recieved replies from vendors and people
wanting a summary of the replies.

{ { {I was wondering what computer programs people are using for 3-D
reconstruction of TEM serial sections. I am interested in peoples opinions
of the software (i.e. ease of use, learning curve, cross-platform
functionality, cost, etc.). Feel free to email me off list if you choose.
Also vendors feel free to send me any info you might have on the products
you sell.} } }

I would really like to hear from the users of these programs! I would be
glad to submit a summary to all interested parties (yes I still have your
addresses).

Thanks to all,

-EK

Eugene W. A. Krueger
Sr. Research Technologist in Cell Biology
Center for Basic Research in Digestive Diseases
Mayo Clinic and Foundation
(507)284-0580 (lab)
(507)284-0762 (fax)
krueger.eugene-at-mayo.edu

see our web page at http://www.mayo.edu/mcniven_lab/


From daemon Wed Sep 26 09:46:03 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Wed, 26 Sep 2001 10:45:48 -0400
Subject: NESM's October 16th Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

Several people have gotten back to me re: the website for the
newsletter. The current (Sept. 2001) newsletter should be posted
very soon on NESM's website--we had some problems with the site! I
apologize for any inconvenience.

The meeting will begin with registration at 5-5:30pm at JEOL USA,
Peabody. The demonstrations will be first and the technical talks
will begin between 7-7:30pm.

Once the newsletter is posted--specific details will be listed. Please contact
Mary McCann, NESM Treasurer, by email: mccanns-at-tiac.net to
pre-register for this meeting.

Peggy Sherwood
Corresponding Secretary, NESM
--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Wed Sep 26 10:13:14 2001



From: Thimothy Schneider :      timothy.schneider-at-mail.tju.edu
Date: Wed, 26 Sep 2001 11:01:23 -0400
Subject: WATER CHILLER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the person asking about water chillers: I have a Haskris water cooled
water chiller that has run for 15 years with out failure. I think that's
about as reliable as a machine can get. Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 JAH
1020 Locust Street
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cell
Timothy.Schneider-at-Mail.TJU.edu



From daemon Wed Sep 26 12:27:31 2001



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 26 Sep 2001 10:16:31 -0700
Subject: organelles in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A researcher has asked if he can examine cell fractions from a gel to
determine which organelles comprise each band in the gel. The bands can be
sucked off and isolated in suspension. Are there any quick techniques to
determine which band is mitochondria, nuclei, etc. I have a Hticahi S3500N
tungsten filament SEM and a cryo stage. How about a negative stain and a
quick look in the TEM?

Thanks,


Rick Harris



From daemon Wed Sep 26 12:30:40 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 26 Sep 2001 13:23:12 -0400 (EDT)
Subject: Re: water recirculators for electron microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I too have high regard for Haskris. We had one that was over 35 years
old and still kicking when we moved to a new lab. We now have a younger
one (maybe 25 yrs old) and a new 2-tank one (2 yrs). They're very
reliable.

..no financial interest in this company, just a satisfied customer.


Sara




On Wed, 26 Sep 2001, Leona Cohen-Gould wrote:

} Date: Wed, 26 Sep 2001 09:34:24 -0400
} From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
} To: Vachik Hacopian {vhacopian-at-wellesley.edu} ,
} microscopy-at-sparc5.microscopy.com
} Subject: Re: water recirculators for electron microscopes
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} }
} } We need to purchase a new water recirculator for our electron microscope
} } (Philips CM-10). Is there a consensus of opinion on what the best
} } recirculator is, reliability being the highest consideration? Thanks.
} }
} } Vachik Hacopian
} ****************
}
} I have a 21 year-old Haskris for my JEOL. It has needed very little
} attention:, mostly water changes when the engineers do my PMs. I had
} to replace the compressor 10 years ago, and just had a leak in the
} refrigerant tubing repaired.
}
} (No financial interest in Haskris or JEOL)
}
} Lee
}
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Sep 26 12:50:45 2001



From: Gang Ning :      gning-at-mcw.edu
Date: Wed, 26 Sep 2001 12:34:06 -0500
Subject: Cryoultramicrotomy of brain cortex

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers:

Somebody wants to do ultramicrotomy of brain cortex. Does any of you
have such experience or literature? Any inputs are greatly appreciated.
TIA.

Gang Ning

EM Facility
Medical College of Wisconsin



From daemon Wed Sep 26 13:12:48 2001



From: Psychology Network :      NetMail-at-pnonline.com
Date: Wednesday, Sep 26 2001 2:04:53 PM EST
Subject: Psychology of the Future

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our system is unique - for the first time, we have combined the power of
the Internet with the ease of the telephone to enable immediate access to
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How does it work? Using our revolutionary new technology, you can reach
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Simply enter your callback phone number and credit card info and you will
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From daemon Wed Sep 26 13:24:16 2001



From: Psychology Network :      NetMail-at-pnonline.com
Date: Wednesday, Sep 26 2001 2:15:42 PM EST
Subject: Psychology of the Future

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our system is unique - for the first time, we have combined the power of
the Internet with the ease of the telephone to enable immediate access to
a psychologist.

How does it work? Using our revolutionary new technology, you can reach
our doctors by clicking on this link:
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Simply enter your callback phone number and credit card info and you will
receive an immediate callback from one of our doctors! Or, just pick up
the phone and call 1-877 DR TALKS (1-877 378-2557)!

Get expert advice on such problems as :

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relationships

stress

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Our service is convenient - we are available 24/7/365.

*Promotional Special: A cruise for two to the Caribbean will be awarded to
one registered client. The winner will be contacted via email. Check our
Website for details.










If you would no longer like to be a part of our mailing list, please accept
our apologies in advance for this email. To be removed, just click this link:
http://dev.pnonline.com/unsubscribe.asp?EGUID=10D7096B-CC89-4DAA-A069-F6E781DDAC28&ELinkID=192.


From daemon Wed Sep 26 18:06:37 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Wed, 26 Sep 2001 20:59:02 -0700
Subject: ultracut E

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------


Anybody wanna sell their Ultracut E? Please respond to Christina Stamper.
"Bennett-stamper, Christina (BENNETCN)" {BENNETCN-at-UCMAIL.UC.EDU}
} You can see I am at a new e mail address as I have just started at the
} Univ of } Cincinnati. I am still looking for an ultracut E. I just wanted
} to let you know } just in case you hear anything...thank you so much.
} Christina Stamper University of Cincinnati EMF operations manager

******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************er




From daemon Thu Sep 27 07:54:42 2001



From: Bert Gough :      agough-at-cellomics.com
Date: Thu, 27 Sep 2001 08:42:07 -0400
Subject: Source for EM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a source of larger sizes of EM grid material. We are
presently using 750 and 1000 mesh grids in 1" squares from SPI. We would
like to get it in 3"x4" size, even if we have to cut it down from a larger
size. SPI says they can supply us with 6x6" but they don't normally stock
that and it takes some time to get it. We would be willing to cut it down.
Does anyone know of other sources of this material? We have tried most of
the standard EM materials suppliers, and they have referred us back to SPI.
Any ideas?

Thanks in advance,

Bert Gough, Ph.D.
Vice President, Systems R&D PH: (412) 826-3600 X-2644
Cellomics, Inc. FX: (412) 826-3850
635 William Pitt Way EM: agough-at-cellomics.com
{mailto:agough-at-cellomics.com}
Pittsburgh, PA 15238
Visit CellSpace(TM) Knowledge Miner: Automated Intelligence for the New
Biology
http://cellspace.cellomics.com {http://cellspace.cellomics.com}



From daemon Thu Sep 27 09:06:10 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 27 Sep 2001 09:57:02 -0400
Subject: Source for EM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did a search on the internet for "electroformed mesh" because I could remember the name of the company that I bought my larger sized grids from. The company is Buckbee Mears. I think that they are also a source of EMP protection for mesh on some of our laminate products for transparencies military aircraft. I have copied a paragraph from one page that has some of the information that you might need to contact them. I once had a catalog from their company, but that was long ago.

BMC Industries, Inc. is a multinational manufacturer and distributor of a variety of products through two segments: Buckbee-Mears and Optical Products. Buckbee-Mears is comprised of Mask Operations and Buckbee-Mears St. Paul (BMSP). Mask Operations produces aperture masks, a critical component of color television and computer monitor picture tubes. BMSP is the leading producer of precision photo-etched metal and electroformed parts. Optical Products designs, manufactures and distributes polycarbonate, glass and hard-resin plastic ophthalmic lenses.

Here is their web address:
http://www.bmcind.com/

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Bert Gough [mailto:agough-at-cellomics.com]
Sent: Thursday, September 27, 2001 8:42 AM
To: microscopy-at-sparc5.microscopy.com
Cc: Anika Joseph; Alex Friedman


We are looking for a source of larger sizes of EM grid material. We are
presently using 750 and 1000 mesh grids in 1" squares from SPI. We would
like to get it in 3"x4" size, even if we have to cut it down from a larger
size. SPI says they can supply us with 6x6" but they don't normally stock
that and it takes some time to get it. We would be willing to cut it down.
Does anyone know of other sources of this material? We have tried most of
the standard EM materials suppliers, and they have referred us back to SPI.
Any ideas?

Thanks in advance,

Bert Gough, Ph.D.
Vice President, Systems R&D PH: (412) 826-3600 X-2644
Cellomics, Inc. FX: (412) 826-3850
635 William Pitt Way EM: agough-at-cellomics.com
{mailto:agough-at-cellomics.com}
Pittsburgh, PA 15238
Visit CellSpace(TM) Knowledge Miner: Automated Intelligence for the New
Biology
http://cellspace.cellomics.com {http://cellspace.cellomics.com}



From daemon Thu Sep 27 09:39:03 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 27 Sep 2001 10:32:38 -0400 (EDT)
Subject: Re: fouled objectives: summary (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The EM supply houses carry special (clean & without grit) styrofoam sticks
for cleaning diamond knives; these are fairly inexpensive. I'm sure these
would be a good source for scratch-free styrofoam with which to clean
lenses.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Sep 27 12:38:27 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Thu, 27 Sep 2001 12:30:35 -0500
Subject: 2nd CSMMS meeting announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A few more notes about the CSMMS meeting on 10/19. If you plan on
attending, please contact me (see below) by October 12. There is no
registration fee for the meeting, but we need to know how many attendees
because lunch will be catered. The lunch is only $5. And if needed, I can
send you a list of hotels and a map for directions.

Caroline Miller will be bring the MSA Traveling Poster Exhibit, Caroline
Schooley will be providing information on Project MICRO and there will be a
live TelePrescence Microscopy connection. There will also be an area for
our Corporate Sponsors and other vendors to discuss their products and
services.

Hope to see you in Columbia.

Lou
____________________

Central States Microscopy and Microanalysis Society Fall Meeting
Friday, October 19, 2001
Adams Conference Center
Veterinary Medicine Building
University of Missouri
Columbia, Mo 65211

8 - 8:45 Registration (Free)

8:50 Welcome

9:00 Tobias Baskin, University of Missouri-Columbia, "Ultrastructure of the
Plant Cell Wall Analyzed with Field-Emission Scanning Electron Microscopy"

9:15 Nadia Navarrete-Tindall, University of Missouri-Columbia, "Morphology
of the Endangered Legume Amorpha nitens Boynton and its Rhizobia Symbiont"

9:30 Heide Schatten, University of Missouri-Columbia, "Cytoskeletal
Dynamics in Apicomplexan Parasites"

9:45 Joe Simmons, University of Missouri-Columbia, "Idiopathic Interstitial
Pneumonia in Laboratory Rats"

10:00 Marty Katz, University of Missouri-Columbia, "Stem Cell
Transplantation Therapy for Inherited Neurodegenerative Disorders"

10:15 Tom Phillips, University of Missouri-Columbia, "Fixation of
Biological Tissues - Science or Art?"

10:30 - 10:45 BREAK

10:45 Robert Hall, Associate Vice Provost of Research, University of
Missouri-Columbia, "The University of Missouri: Taking the Lead in National
Research Funding"

11:00 Keynote speaker: Kenneth Moore, University of Iowa, MSA Tour Speaker,
"Applications of Microscopy Techniques to the Study of Genetic Therapy
Research"

12:00 - 1:00 Lunch

1:00 - 1:30 CSMMS Business Meeting

1:30 John Bozzola, SIU-Carbondale, "Multidisciplinary Approach to Teaching
Electron Microscopy"

1:45 Howard Wilson, University of Missouri-Columbia, "Creating a Scientific
Poster Presentation using Microsoft Powerpoint"

2:00 Scott Walck, PPG, "Low Angle Cleavage - A New Specimen Preparation
Method for TEM"

2:15 Louis Ross, University of Missouri-Columbia, "Exciting Times in
Scanning Electron Microscopy and X-ray Microanalysis"

2:30 Vladimir Dusevich, University of Missouri-KC, "Practical ESEM"

2:45 Jeff Speakman, University of Missouri-Columbia, "Laser Ablation ICP-MS
as a Tool for Characterization of Archaeological Materials"

3:00 - 3:15 BREAK

3:15 Cammy Bright, University of Missouri-Columbia, "A Laser Ablation
ICP-MS and SEM Study of Rare Earth and Trace Elements in Conodonts"

3:30 Mike Amspoker, Westminster College, "A Light and Scanning Electron
Microscopy Study of the Freshwater Diatom Desmogonium rabenhorstianum
Grunow"

3:45 Heather Ramsay, University of Missouri-Columbia, "New Applications of
SEM in Osteological Morphology Research"

4:00 Dickerson Moreno, University of Missouri, "Development of N-Type
Diamond Semiconductor Through Field Enhanced Diffusion by Optical
Activation"

4:15 Alejandro Suarez, University of Missouri, "A New Method to Fabricate
Polycrystalline Diamond as a P-Type Semiconductor"

4:30 Closing Remarks
_______________________




Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.bioterc.missouri.edu/emc


From daemon Fri Sep 28 08:08:32 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 28 Sep 2001 08:51:50 -0400
Subject: Re: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just came across a web site that may help this discussion. I know that I
can't address the needs of others, but the Wadsworth Center appears to be
serious about the methods and the approach. URL:
http://www.wadsworth.org/rvbc/index.html

Also the HVEM at Boulder has a 3-D Subdivision with software available:
URL: http://bio3d.colorado.edu/index.html

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Fri Sep 28 08:39:35 2001



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Fri, 28 Sep 2001 08:34:29 -0500
Subject: TEM Ink Jet Printer Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists,

We have recently put a digital camera on our TEM. We have worked out most of
the problems related to obtaining accurate and precise measurements from the
digital images on the monitor. Now I must deal with printer paper.

I believe we have 2 different printing needs. First, we will be printing
hundreds of TEM images per year that do not have to be high quality but will be
filed away for 10-20 years. Second, we will hopefully need to print several
publication quality prints per year for submission with manuscripts. We have an
EPSON Sylus 980 ink jet printer and a long list of possible printer papers.
Before I buy many different papers to test, I would appreciate opinions
regarding which papers will fill our 2 needs.

Thanks for your help,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Fri Sep 28 11:27:54 2001



From: David DeRosier :      derosier-at-brandeis.edu
Date: Fri, 28 Sep 2001 12:21:11 -0400
Subject: Large Structures Meeting-Asilomar-April, 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may have to find better ink that what is normally supplied if you want
the images to last years, or get an (relatively) inexpensive laser printer
like the NEC. Even 600dpi yields decent images from our Philips XL 20. I
believe the newer model NEC (870?) does much better .

Ron
----- Original Message -----
} From: "John Basgen" {basgen-at-maroon.tc.umn.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 28, 2001 9:34 AM


The Biophysical Discussions
...........................................................................
..........
Frontiers in Structural Cell Biology:
Determining the structures of large subcellular machines
...........................................................................
..........
Meeting Dates: April 20-22, 2002.

Meeting Site: Asilomar, California. This is a spectacular site.
You can see it at http://www.asilomarcenter.com/.
...........................................................................
..........
The biophysical discussions are set up so that the discussion time is
greater than the times allotted for presentations, which will be available
on the web prior to the meeting. The format is unusual, but highly
successful in the past.

To apply send a letter of intent to discussions-at-biophysics.org by November
15, 2001. Space is limited.
...........................................................................
..........
Organizing committee: Axel Brunger (Stanford University), David DeRosier
(Brandeis University), Steve Harrison (Harvard University), and Eva Nogales
(University of California at Berkeley).

The Program (Saturday, April 20 at 9 am to Monday April 22 at 12 noon)

Session I. The state of structural biology of large structures.
Moderator Helen Saibil
Richard Henderson
The power of electron cryomicroscopy.
Joachim Frank
The Ribosome -- a molecular machine in motion
Jamie Cate
Biochemical basis for x-ray crystallography of the
ribosome

Session II. Extending x-ray crystallography to ever larger structures
Moderator Keith Hodgson
Andy Thompson
Can we routinely collect useful data from
micro-crystals?
Janos Hajdu
Future X-ray sources (tentative)
Randy Reed
The phase problem: does size matter?

Session III. New ways to obtain large complexes for structural studies
Moderator Axel Brunger
Don Wiley
Stabilizing multi-component biological complexes for
structural studies by protein engineering, expression, and refolding - AND -
avoiding artifacts
TBA
Expression and Co-expression of components
(tentative title)


Session IV. What does the future hold for electron cryomicroscopy?
Moderator Bob Glaeser
Niko Grigorieff
Single particles always fit the mold
Ken Downing
The hybrid approach to electron crystallography
Wolfgang Baumeister
Electron Tomography: Towards visualizing
macromolecular assemblies
inside cells
Ed Egelman
Polymorphism, can we detect it? Can we use it? Can we control it? Examples
from actin and nucleoprotein complexes.

Session V. Can hybrid methods provide credible atomic models?
Moderator Eva Nogales
Niels Volkmann
Atomic model of the cell: docking in a tomographic
environment
Willy Wriggers
Reconciling Shape with Structure: Morphometric Strategies for
Multi-Resolution Flexing



From daemon Fri Sep 28 13:36:15 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Fri, 28 Sep 2001 11:27:46 -0700
Subject: RE: TEM Ink Jet Printer Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John,
The goal of linear, archival, high-quality gray scale images with a
large dynamic range can be difficult to achieve. One option is a Codonix
printer - they produce pretty stunning photo-quality prints, but they are pretty
expensive. Another option might be to go with a quad-black print system. There
are several available for Epson ink jet printers, and I have had good success
with one of these (sold by MIS Associates) set ups on an Epson 1200.
You might want to check out InkJet Mall
(http://www.inkjetmall.com/store/index.html) , and see what they have to offer.
I am not affiliated with them, but ran across them as a vendor. They work with
the art industry, and have developed a proprietary photoshop pluggin, printer
driver, and inkset that is supposed to give outstanding gray scale images. I
just ordered a system, and it will be arriving shortly. Its called piezography
BW.
As far as papers go, you might want to check out LumiJet Preservation
Series Gallery Gloss. I've tested a number of different papers with pigmented
inks, and that's the one that I liked the best. Some vendors that I have used
in the past are: InkjetArt.com at:
http://www.tssphoto.com/sp/dg/luminos/index.html, and MIS Associates at:
http://www.inksupply.com/

Good luck.

-Brad


----------
From: John Basgen
Sent: Friday, September 28, 2001 06:34
To: Microscopy-at-sparc5.microscopy.com
Subject: TEM Ink Jet Printer Paper

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Microscopists,

We have recently put a digital camera on our TEM. We have worked out
most of
the problems related to obtaining accurate and precise measurements from
the
digital images on the monitor. Now I must deal with printer paper.

I believe we have 2 different printing needs. First, we will be
printing
hundreds of TEM images per year that do not have to be high quality but
will be
filed away for 10-20 years. Second, we will hopefully need to print
several
publication quality prints per year for submission with manuscripts. We
have an
EPSON Sylus 980 ink jet printer and a long list of possible printer
papers.
Before I buy many different papers to test, I would appreciate opinions
regarding which papers will fill our 2 needs.

Thanks for your help,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu





From daemon Fri Sep 28 18:42:46 2001



From: Meghan Towner :      town3648-at-uidaho.edu
Date: Fri, 28 Sep 2001 16:35:13 -0700 (PDT)
Subject: pre-perfusion with saline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!
I would like to settle an on-going debate about pre-perfusion with saline. Does anyone pre-perfuse with saline before perfusion with fixative for CNS tissue?
Thank you for your help.
Meghan Towner
University of Idaho



From daemon Fri Sep 28 19:50:32 2001



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Fri, 28 Sep 2001 19:43:17 -0500
Subject: Used Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!

We are looking for a used ultramicrotome for our materials science
lab. If anyone has one they are looking to see, or if anyone knows
where to find one, could you please contact me.

Thanks,

Praveena Bhaskara

Graduate student

Department of chemical engineering

University of Massachusetts,Lowell

978-934-3411





Praveena Bhaskara



Get your FREE download of MSN Explorer at
{http://go.msn.com/bql/hmtag_itl_EN.asp} http://explorer.msn.com


From daemon Sat Sep 29 04:27:48 2001



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 29 Sep 2001 02:17:14 -0700 (PDT)
Subject: Re: TEM Ink Jet Printer Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

For my TEM digital system, I have a HP 960 inkjet printer ($250.00) with
2600 x 1200 dpi quality. The paper I am currently using is HP Premium
Paper at ~$50.00 for 50 sheets. I have tried to get a better deal by
purchasing in bulk, but I was told by our supplier (Corporate Express) Hp
does not sell in bulk and only sell the pre-packaged 50 sheet units.

I am still in the process of evaluating other products/manufacturers of
photo quality paper.

Ken


--
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Director, Imaging Center
Legacy Holladay Park Medical Center
1225 NE 2nd Avenue
Portland, OR 97232


On Fri, 28 Sep 2001, John Basgen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Microscopists,
}
} We have recently put a digital camera on our TEM. We have worked out most of
} the problems related to obtaining accurate and precise measurements from the
} digital images on the monitor. Now I must deal with printer paper.
}
} I believe we have 2 different printing needs. First, we will be printing
} hundreds of TEM images per year that do not have to be high quality but will be
} filed away for 10-20 years. Second, we will hopefully need to print several
} publication quality prints per year for submission with manuscripts. We have an
} EPSON Sylus 980 ink jet printer and a long list of possible printer papers.
} Before I buy many different papers to test, I would appreciate opinions
} regarding which papers will fill our 2 needs.
}
} Thanks for your help,
}
} John
}
} John M. Basgen
} Department of Pediatrics
} University of Minnesota
} Mayo Mail Code 491
} 420 Delaware Street SE
} Minneapolis, MN 55455
} USA
} Phone: 612-625-7979
} FAX: 612-626-2791
} E-mail: basgen-at-umn.edu
}
}





From daemon Sat Sep 29 11:13:39 2001



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Sat, 29 Sep 2001 11:59:15 -0400
Subject: ink jet paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John, Ken, and anyone else

For high-definition ink-jet paper try Jet Print Imaging
from International Paper. It is better the HP Premium
and cheaper. It comes in reams (only) of 500 at $175,
which $0.35/sheet. Cadmet is one of the distributors.

JQuinn

PS: They will also send you a sample pack.

} From Microscopy-request-at-sparc5.microscopy.com Sat Sep 29 09:32:49 2001
} Date: Sat, 29 Sep 2001 02:17:14 -0700 (PDT)
} From: Ken Tiekotter {tiekotte-at-up.edu}
} To: John Basgen {basgen-at-maroon.tc.umn.edu}
} cc: {Microscopy-at-sparc5.microscopy.com}
} Subject: Re: TEM Ink Jet Printer Paper
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear John,
}
} For my TEM digital system, I have a HP 960 inkjet printer ($250.00) with
} 2600 x 1200 dpi quality. The paper I am currently using is HP Premium
} Paper at ~$50.00 for 50 sheets. I have tried to get a better deal by
} purchasing in bulk, but I was told by our supplier (Corporate Express) Hp
} does not sell in bulk and only sell the pre-packaged 50 sheet units.
}
} I am still in the process of evaluating other products/manufacturers of
} photo quality paper.
}
} Ken
}
}
} --
} Ken Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N. Willamette Blvd.
} Portland, OR 97203
}
} Director, Imaging Center
} Legacy Holladay Park Medical Center
} 1225 NE 2nd Avenue
} Portland, OR 97232
}
}
} On Fri, 28 Sep 2001, John Basgen wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Microscopists,
} }
} } We have recently put a digital camera on our TEM. We have worked out most of
} } the problems related to obtaining accurate and precise measurements from the
} } digital images on the monitor. Now I must deal with printer paper.
} }
} } I believe we have 2 different printing needs. First, we will be printing
} } hundreds of TEM images per year that do not have to be high quality but will be
} } filed away for 10-20 years. Second, we will hopefully need to print several
} } publication quality prints per year for submission with manuscripts. We have an
} } EPSON Sylus 980 ink jet printer and a long list of possible printer papers.
} } Before I buy many different papers to test, I would appreciate opinions
} } regarding which papers will fill our 2 needs.
} }
} } Thanks for your help,
} }
} } John
} }
} } John M. Basgen
} } Department of Pediatrics
} } University of Minnesota
} } Mayo Mail Code 491
} } 420 Delaware Street SE
} } Minneapolis, MN 55455
} } USA
} } Phone: 612-625-7979
} } FAX: 612-626-2791
} } E-mail: basgen-at-umn.edu
} }
} }
}
}
}
}


From daemon Sat Sep 29 13:32:15 2001



From: Harrison :      tuttle-at-home.com
Date: Sat, 29 Sep 2001 13:22:24 -0500
Subject: Re: TEM Ink Jet Printer Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you're referring to part number C6979A, HP premium glossy photo
paper, it is available for less
than half that at many retailers.

Regards,
Dave Harrison




On 29 Sep 2001, at 2:17, Ken Tiekotter wrote:

}
} For my TEM digital system, I have a HP 960 inkjet printer ($250.00) with
} 2600 x 1200 dpi quality. The paper I am currently using is HP Premium
} Paper at ~$50.00 for 50 sheets. I have tried to get a better deal by
} purchasing in bulk, but I was told by our supplier (Corporate Express) Hp
} does not sell in bulk and only sell the pre-packaged 50 sheet units.
}


From daemon Sun Sep 30 04:38:57 2001



From: tonino :      t.train-at-tin.it
Date: Sun, 30 Sep 2001 11:39:12 +0200
Subject: Help to stick the dental plaque on glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I'm trying to identify Helicobacter pylori in the dental plaque using IHC
(immunoenzymatic/immunohistochemicals) staining techniques with primary
antibody from rabbit.
For the observations I'm using SEM thanks to the reaction between oxidated
DAB and Osmium that forms an electondense compound.Unfortunately the
adherence of bacterium to glass seems to be an insurmountable obstacle.
Does somebody know any quick and strong techniques to "stick" on glass cover
slip the dental plaque?
thancks in advance
T. Train



From daemon Sun Sep 30 04:38:57 2001



From: ttrain-at-tin.it
Date: Sun, 30 Sep 2001 11:29:01 CEST
Subject: help for dental plaque on glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I'm trying to identify Helicobacter pylori in the dental plaque using IHC (immunoenzymatic/immunohistochemicals) staining techniques with primary antibody from rabbit.
For the observations I'm using SEM thanks to the reaction between oxidated DAB and Osmium that forms an electondense compound.Unfortunately the adherence of bacterium to glass seems to be an insurmountable obstacle.
Does somebody know any quick and strong techniques to "stick" on glass cover slip the dental plaque?
thancks in advance
T. Train



From daemon Sun Sep 30 04:42:48 2001



From: :      ttrain-at-tin.it
Date: Sun, 30 Sep 2001 11:43:36 +0200
Subject: I: Help to stick the dental plaque on glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I'm trying to identify Helicobacter pylori in the dental plaque using IHC
(immunoenzymatic/immunohistochemicals) staining techniques with primary
antibody from rabbit.
For the observations I'm using SEM thanks to the reaction between oxidated
DAB and Osmium that forms an electondense compound.Unfortunately the
adherence of bacterium to glass seems to be an insurmountable obstacle.
Does somebody know any quick and strong techniques to "stick" on glass cover
slip the dental plaque?
thancks in advance
T. Train



From daemon Sun Sep 30 11:02:20 2001



From: Bennett Goldberg :      goldberg-at-bu.edu
Date: Sun, 30 Sep 2001 11:54:47 -0500
Subject: Phillips CM12 Available from Boston University Physics Dept.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Boston University Physics Department is upgrading its Materials Research
Facility and looking for a buyer for its existing STEM. Please contact:
Prof. Bennett Goldberg
Boston University Physics Department
617-353-5789 goldberg-at-bu.edu

-------------------------------------
Philips CM12 (120 keV) "Twin Lens" Electron Microscope
with Software Upgrade, S/N D733 purchased 1988
STEM Unit Model 5227 (for CM10/CM12), S/N D733.1
Specimen Relocation System Model 5235, S/N D733.2
Backscatter detector (for selecting high Z)
Secondary electron detector (for 5-nm-imaging)
Gatan film camera (for conventional "photography")
Various Gatan sample holders
Haskris Cooling Water Heat Exchanger
EDAX Energy Dispersive Analyzer, Model PV9800,
S/N HX-626/01

The microscope was used for conventional electron microscopy,
5-nm-resolution Scanning Electron Microscopy, and 10-nm resolution
electron-beam lithography. The lithography limitations were set by the
maximium chip size (approx. 5 mm by 8 mm), the typical field of view while
writing (80 micrometers), and the mechanical relocation alignment accuracy
(0.25 micrometers).

The system does not have a beam blanker, but has been equipped with a
carefully shielded "x-y" input. Our homemade pattern definition system is
obsolete and not included.

The microscope was under service contract, fully operational, and checked
out by the Philips/FEI preventative maintenance service several times each
year until it was decommisioned last year.

---
Bennett B Goldberg
Professor of Physics
Boston University
590 Commonwealth Ave
Boston, MA 02215
(617) 353-5789/ 1712 lab/ 9917 fax
goldberg-at-bu.edu
http://ultra.bu.edu



From daemon Mon Oct 1 02:41:03 2001



From: Peter Heimann :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Mon, 01 Oct 2001 09:27:47 +0200
Subject: Re: pre-perfusion with saline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



from my experience with mouse (CNS; spinal chord, muscle, testis
and other tissue) I would strongly recommend a short (!) pre-
perfuse (as you name it) to flush out all blood cells, which
otherwise regularly clog and occlude the fine capillaries.
However, though simple saline flush can be sufficient, I routinely
use a mixture of Heparin (anti-coagulant), Procain (keeps the blood
vessels "open"), PVP (polyvinylpyrrolidon, MW-class 30 K; for
osmotic pressure) in aqua dest. and pH-adjustment.
Method published originally in a wonderful perfusion-technique
paper by Forssmann, WG et al (1977) in Anat. Rec. 188: p 307-
314. Just follow their technical procedure exactly and you`ll get
excellent fixation.

good luck!

peter



**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Mon Oct 1 02:54:50 2001



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Mon, 01 Oct 2001 09:49:41 +0200
Subject: Re: 3D reconstruction from serial sections, 2nd request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugene,

Sorry for being rather late with my reaction, but – as far as I have seen –
nobody has given a hint relating the home of “3-Dimensional Electron Microscopy”
(http://3dem.sdsc.edu/). This page collects information about the laboratories
and services available to the 3D-EM community. A lot of available reconstruction
packages are listed at this page. Additionally there exists also a listserver
for specific questions/problems.

I hope this helps.

Best wishes,
Ingo

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
Herbststrasse 7
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: http://www.tvips.com/
Email: ingo.daberkow-at-tvips.com
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++


"Krueger, Eugene W." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello to all in the microscopy community,
}
} I have previously posted a request for information on 3D reconstruction from
} serial sections.....but I only recieved replies from vendors and people
} wanting a summary of the replies.
}
} { { {I was wondering what computer programs people are using for 3-D
} reconstruction of TEM serial sections. I am interested in peoples opinions
} of the software (i.e. ease of use, learning curve, cross-platform
} functionality, cost, etc.). Feel free to email me off list if you choose.
} Also vendors feel free to send me any info you might have on the products
} you sell.} } }
}
} I would really like to hear from the users of these programs! I would be
} glad to submit a summary to all interested parties (yes I still have your
} addresses).
}
} Thanks to all,
}
} -EK
}
} Eugene W. A. Krueger
} Sr. Research Technologist in Cell Biology
} Center for Basic Research in Digestive Diseases
} Mayo Clinic and Foundation
} (507)284-0580 (lab)
} (507)284-0762 (fax)
} krueger.eugene-at-mayo.edu
}
} see our web page at http://www.mayo.edu/mcniven_lab/



From daemon Mon Oct 1 07:13:03 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Mon, 1 Oct 2001 08:25:20 -0400
Subject: EDX Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The only two brands I've had personal experience with are Haskris and Neslab
chillers. Both seem pretty good - usually the first problem you run into
(hopefully only after several years' service) is the pump failing. In these
two brands the pumps are often identical, made by a third company. I think I
prefer the Haskris machines, though - their reservoirs tend to be readily
openable for inspection - most seem to have square tanks with a lid that you
can easily lift off to see what's going on inside. The Neslab chiller we
have now has a cylindrical, sealed tank, so it can be a bit more difficult
to troubleshoot. Hope this helps.

Frank Thomas
GSC Atlantic
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
----- Original Message -----
} From: "Vachik Hacopian" {vhacopian-at-wellesley.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, September 25, 2001 3:24 PM


Dear Fellow Microscopists;

I have a question relative to the detection of Tl [Thallium] in the presence
of a Au plated surface. The application of this plated region is the
metallization system of a microcircuit. SIMS analysis was performed on
these Au plated surfaces and found the presence of thallium in the several
atm. %. However, when I attempt to detect it using EDX, I cannot. I have
looked at the energy spectra and found that they separate out well in the
higher energies yet I see absolutely no indication of thallium. The
thickness of the sample is ~ 1.0 uM and about 100 um X 100 uM in area.
Plenty enough for this analysis.

Does anyone know of any unique situation with thallium in gold? Either the
SIMS analysis is in error or I'm overlooking something.

Peter Tomic
Analytical Services
Anadigics, Inc.
Warren, New Jersey


From daemon Mon Oct 1 08:01:07 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Mon, 1 Oct 2001 07:43:53 -0500
Subject: RE: EDS procedures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check out ASTM standards. ...Available for both Quant EDS and Magnification
for SEMs.

Woody


} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone know of any documented EDS calibration (energy display,
} quantification, etc.) procedures that have been certified in any way?
} i.e. NIST, ISO-9000 or any other.
}
} thanks,
}
} Mark Wall
} LLNL
} 925 423 7162
}


From daemon Mon Oct 1 10:29:34 2001



From: David J. DeRosier :      derosier-at-brandeis.edu
Date: Mon, 1 Oct 2001 11:17:12 -0400
Subject: postdoctoral position in electron cryomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Opportunities for Postdoctoral Fellows in Electron Cryomicroscopy and
Structural Biology
Prof. David DeRosier (derosier-at-brandeis.edu).
Rosenstiel Basic Medical Sciences Research Center
Brandeis University

I have a postdoctoral position to study the structure of large
macromolecular machines. The position is fully funded for at least two
years, and we will pay competitive salaries. Write me if you or someone you
know is interested. Here is a brief description of the available projects:

The cytoskeleton:
The actin cytoskeleton is a set of cellular machines responsible for many
of the dynamic capabilities of eukaryotic cells. Actin bundles are a key
elements used to shape cells, generate filopodia, and order the cell
cytoplasm. The project is aimed at determining the structure of the actin
bundle in the brush border cells, as a model system. These bundles are the
best characterized and most tractable bundles. The approach is two pronged
beginning with in vitro studies of the components (actin, fimbrin and
villin) and leading to in vivo studies of intact bundles. The tool we use
is electron cryomicroscopy and image analysis.

The bacterial flagellum:
The flagellum is a several machines in one. It is composed of over a dozen
different proteins, with one set making up the rotary motor, another the
drive train, another a bushing and seal, and another the flagellar export
apparatus. We determining the structures of these components using
electron cryomicroscopy and image analysis, and we are correlating
structural features with the primary sequences of the component proteins.

The bacterial signaling complex:
The bacterial cell senses the environment and transmits a signal to the
flagellum. We are using electron cryomicroscopy and image analysis to
determine the structure of a 1.4 megadalton complex of the cytoplasmic
domain of a chemoreceptor, a kinase, and an adaptor molecule. The complex
is highly active enzymatically, but has an unusual stoichiometry, which
suggests signal integration from several receptors into a single kinase.


Professor David J. DeRosier
Abraham S. and Gertrude Burg Chair of Life Sciences
W.M. Keck Institute for Cellular Visualization
Rosenstiel Basic Medical Sciences Research Center
MS029
Brandeis University
415 South Street
Waltham, MA 02454-9110
Telephone: 781-736-2494
FAX: 781-736-2405
email: derosier-at-brandeis.edu




From daemon Mon Oct 1 10:50:06 2001



From: Joyce Craig :      J-Craig-at-csu.edu
Date: Mon, 01 Oct 2001 10:36:14 -0700
Subject: pre-perfusion with saline

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you don't wash the blood out by pre-perfusion the blood will fix and
clog up the vascular system so the perfusion will stop, won't it?
Joyce



From daemon Mon Oct 1 12:42:01 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 01 Oct 2001 13:32:42 -0400
Subject: Chatt(er)ing about MT-7 Repairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,

I need to find someone in the Washington, DC area (or close by) who repairs RMC ultramicrotomes. We have an MT-7 that has a very fine chatter visible only under the beam.

If you know of anyone/company that does this type or work, please let me know.

I don't mind chatter, just not on my sections.

Paula :-)

p.s. hey sectioner, sectioner.....Breathe!

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Oct 1 15:07:30 2001



From: Ruth Yamawaki :      Ryamawaki-at-cmexchange.stanford.edu
Date: Mon, 1 Oct 2001 12:57:57 -0700
Subject: Parts for JEOL 100CX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are looking for a specimen rotating holder for a JEOL 100CX part#
IEM100/200CX-SRH. Also for the JEOL 100CX we are in need of a dispensing
magazine, receiving magazine and/or cassette film holders. If you are
looking for a home for any of these items please contact me.

Thank you,

Ruth Yamawaki
Department of Comparative Medicine
Stanford University
(650) 723-3457
FAX (650) 498-6259


From daemon Tue Oct 2 05:57:49 2001



From: Nazlia :      ctssamodien-at-samiot.uct.ac.za
Date: Tue, 2 Oct 2001 12:41:43 +0200
Subject: Diamond Knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All
I need to purchase a new 3mm / 5mm Diamond knife for ultrathin
sections of calcified tissue and polyurethane samples in Spurs resin.
The prices from most of the suppliers are horrendous, as you well
know. Does anyone out there have any of these lying around which are
not used? As a last resort we'll consider a good, used one.Any brand
name would do. And where can I get my "old" one re-sharpened at a
reasonable price?
Thanks and regards
Nazlia


From daemon Tue Oct 2 08:20:37 2001



From: Judy Trogadis :      trogadisj-at-smh.toronto.on.ca
Date: Tue, 02 Oct 2001 09:10:59 -0400
Subject: image collection package

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a high end deconvolution program that would also include image acquisition capabilities. I colleague would like to do both with a single software package.

Any suggestions would be appreciated. Thank you.

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Tue Oct 2 10:04:01 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Tue, 2 Oct 2001 11:15:45 -0400
Subject: RE: MT-7 repairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula:
For many years we have relied on two firms for ultramicrotome maintenance
and repair work. Both firms we found could be counted on for prompt and
excellent work. For our MT-series ultramicrotomes we have engaged the
services of Bill McGee of Microtome Service Co. (315)-451-1404. For our
Reichert and LKB instruments we relied on Helmut Ptzig and Mario Saccoccio
of Microscopical Optical Consulting Inc. (914) 268-6450 or e-mail
MOCLeica-at-aol.com.

Its possible that the chatter is not being generated from the
ultramicrotome, but from some outside source of vibration. If so, you may
want to install an anti-vibration platform.
Regards,
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
State University of New York-Binghamton
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu



From daemon Tue Oct 2 10:07:34 2001



From: Caroline Miller :      camiller-at-creighton.edu
Date: Tue, 02 Oct 2001 10:03:33 -0500
Subject: Ammonium chloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Morning,
Does anyone out there know why you add ammonium chloride to a pre
incubating step? Caroline Miller



From daemon Tue Oct 2 19:46:24 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 3 Oct 2001 12:34:53 GMT+1200
Subject: JEOL X-Ray Counting Unit info reqd

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi there

I have inherited a 1980s JEOL X-Ray Counting Unit Type XCU, which I
want to press into service as a ratemeter for wds alignment purposes.

Is anyone out there sufficiently familiar with this model and
sufficiently generous with their time to reply to me off-list so that
I can be enlightened?

thanks

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Tue Oct 2 20:12:44 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Wed, 3 Oct 2001 11:08:52 +1000
Subject: RE: Diamond Knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Diamond knife manufacturers are mostly located in Switzerland and USA and they
will tell you that prices have remained constant or decreased over the past 20
years. But you are right too, countries whose currencies have not kept pace
with the US$, the Swiss Franc and the Euro find diamond knives and many other
imported items very expensive indeed.
The Australian dollar 2 years ago was equal to US 65 cents. Now it is 48.5
cents; a decline of 25%
at a time of lower inflation here than in the USA. When our business was
started 22 years ago we received US$1.20 for one A$.
This topic is peripheral to the listserver, but the exchange rate woes do
affect numerous microscopists. There is nothing we can do, but to encourage
microscopists in the high exchange rate countries to come and visit our
country. Australia is cheap for you to travel in, as our $ buys as much of most
Australian made goods as the US$ buys in the USA. We have an excellent
conference planned in February 2002 in Adelaide.
Spend your money here and support our A$ !
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, October 02, 2001 8:42 PM, Nazlia
[SMTP:ctssamodien-at-samiot.uct.ac.za] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello All
} I need to purchase a new 3mm / 5mm Diamond knife for ultrathin
} sections of calcified tissue and polyurethane samples in Spurs resin.
} The prices from most of the suppliers are horrendous, as you well
} know. Does anyone out there have any of these lying around which are
} not used? As a last resort we'll consider a good, used one.Any brand
} name would do. And where can I get my "old" one re-sharpened at a
} reasonable price?
} Thanks and regards
} Nazlia


From daemon Wed Oct 3 08:36:23 2001



From: Rudy De Vos :      Rudy.DeVos-at-mtm.kuleuven.ac.be
Date: Wed, 3 Oct 2001 15:17:41 +0200
Subject: spin-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I can't find compagnies which have spin-polarization detectors (magnetic
microstructures in SEM) in their sales program. Does anyone out there can
help.

Ing. Rudy De Vos
K.U.Leuven - Department MTM
Kasteelpark Arenberg 44
B - 3001 Heverlee
Belgium


From daemon Wed Oct 3 08:39:42 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 3 Oct 2001 09:28:22 -0400
Subject: Re: Ammonium chloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Good Morning,
} Does anyone out there know why you add ammonium chloride to a pre
} incubating step? Caroline Miller
******************
It quenches fluorescence from unreacted aldehydes.

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Oct 3 09:03:08 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 3 Oct 2001 08:42:26 -0500
Subject: Re: Ammonium chloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you are trying to bind up all the free aldehyde groups left over from
fixation with aldehydes so they don't react with your antibodies.
glycine works too. tom



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Oct 3 09:16:24 2001



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Wed, 3 Oct 2001 15:11:41 +0100 (BST)
Subject: Confocal listserve URL wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
Could someone please send me the confocal istserve URL
for subscription to that list.
Many thanks,
Jeremy
jb_sanderson-at-yahoo.com

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie


From daemon Wed Oct 3 11:09:16 2001



From: David_R_Stadden-at-armstrong.com
Date: Wed, 3 Oct 2001 12:01:31 -0400
Subject: East coast repair for B&L scope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Anyone know of a good repair facility in PA or close by for a Bausch & Lomb
Stereozoom 7? I'm told the focusing rack is messed up. Appreciate any leads.

Dave Stadden
Armstrong World Industries, Inc.
717-396-5109
drstadden-at-armstrong.com




From daemon Wed Oct 3 12:27:44 2001



From: FABBRI :      fabbri-at-cigssrv1.unimo.it
Date: Wed, 3 Oct 2001 19:19:49 +0200
Subject: Oxford INCA 100 EDS file format

Contents Retrieved from Microscopy Listserver Archives
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Does anyone give me informations about Oxford INCA 100 EDS file format?
Any reference for an INCA {--} EMS spectra converter?

P.L. Fabbri

---------------------------------------------
Dr. Pier Luigi Fabbri
C.I.G.S. - Centro Interdip. Grandi Strumenti
Università di Modena
via Campi 213/A - 41100 Modena, Italy
Tel +39-059-2055231 / +39-059-370551
Fax +39-059-370551
E-mail: fabbri-at-mail.cigs.unimo.it
---------------------------------------------





From daemon Wed Oct 3 13:29:16 2001



From: akc-at-umich.edu
Date: Wed, 03 Oct 2001 14:25:22 -0400
Subject: Re: Ammonium chloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As has been pointed out, ammonium chloride reduces aldehyde double bonds so
they won't react with your antibodies. Another advantage of reducing
double bonds is to cut down the amount of autofluorescence in your specimen
(in other words, places that fluoresce nonspecifically, because of double
bonds). Ammonium chloride is relatively gentle in this regard. If your
specimen has strong autofluorescence, you may need to resort to a more
drastic procedure involving sodium borohydride (which is toxic, so has to
be handled carefully). A good article that deals with sodium borohydride
and bond reduction is:

Eldred et al, 1983, Comparison of fixation and penetration enhancement
techniques for use in ultrastructural immunocytochemistry, J Histochem
Cytochem 31:285-292.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Oct 3 13:29:18 2001



From: Everett Ramer :      eramer-at-cellomics.com
Date: Wed, 3 Oct 2001 14:20:20 -0400
Subject: How to determine shading of fluorescence microscope image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there a procedure for determining the shading of a fluorescence
microscope image? I assume that one needs to take an image of a uniform
object, but are there standard objects? Will a volume object work, or must I
use a surface object. Would a front-surface mirror work, assuming I could
measure the leakage through the barrier filter?
Everett Ramer


From daemon Wed Oct 3 14:36:59 2001



From: wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 03 Oct 2001 14:34:16 -0500
Subject: replicas vs. FE-SEM

Contents Retrieved from Microscopy Listserver Archives
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To my understanding (which is admittedly weak in this area) the preferred
method for visualizing membrane structure is to use freeze-fracture, then
carbon replica, then TEM. Does anyone have an opinion as to whether high
resolution FE-SEM of metal-coated samples would be as effective in this regard
as viewing a replica in the TEM.

Bob

Robert R. Wise, Ph.D.
University of Wisconsin-Oshkosh

Currently on sabbatical at UW-Madison
Botany Department
430 Lincoln Drive
Madison, WI 53706
email: wise-at-uwosh.edu
phone: 608-262-4288
fax: 608-262-7509



From daemon Wed Oct 3 16:38:43 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 3 Oct 2001 17:29:37 -0400 (EDT)
Subject: Re: East coast repair for B&L scope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TekNet has fixed several of our binocs. Email Jon Petz and give him your
model info, etc., to see if he can work on it. He's in your area.

jon-at-teknetinc.com

Sara

On Wed, 3 Oct 2001 David_R_Stadden-at-armstrong.com-at-sparc5.microscopy.com wrote:

} Date: Wed, 3 Oct 2001 12:01:31 -0400
} From: David_R_Stadden-at-armstrong.com-at-sparc5.microscopy.com
} To: Microscopy-at-sparc5.microscopy.com
} Subject: East coast repair for B&L scope?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Anyone know of a good repair facility in PA or close by for a Bausch & Lomb
} Stereozoom 7? I'm told the focusing rack is messed up. Appreciate any leads.
}
} Dave Stadden
} Armstrong World Industries, Inc.
} 717-396-5109
} drstadden-at-armstrong.com
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Oct 3 18:36:58 2001



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Wed, 3 Oct 2001 16:28:41 -0700 (PDT)
Subject: Re: East coast repair for B&L scope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try the Leica Inc. facility in Buffalo NY and ask for Ken Brown. I think
he can help you. The phone number is (716)686-3000 or if needed the main
Leica # 1-800-248-0123. Leica owns B&L and handles parts for these
microscopes.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seatle, WA 98195
(206)543-7702



On Wed, 3 Oct 2001 David_R_Stadden-at-armstrong.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Anyone know of a good repair facility in PA or close by for a Bausch & Lomb
} Stereozoom 7? I'm told the focusing rack is messed up. Appreciate any leads.
}
} Dave Stadden
} Armstrong World Industries, Inc.
} 717-396-5109
} drstadden-at-armstrong.com
}
}
}
}



From daemon Thu Oct 4 00:59:31 2001



From: Pankaj Kumar Patro :      pankaj-at-met.iitb.ac.in
Date: Thu, 4 Oct 2001 11:22:15 +0000 (IST)
Subject: Etchant for Zr

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Dear Listner..

We have deposited Al/Zr Multilayer on Zr subtrate.For the TEM
analysis..we r in need of a prper etchent of Zr.If any one has some idea
please reply...

Thanks in advance
regards
pankaj





From daemon Thu Oct 4 02:29:55 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 4 Oct 2001 08:31:12 +0100 (GMT Daylight Time)
Subject: Re: Oxford INCA 100 EDS file format

Contents Retrieved from Microscopy Listserver Archives
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Hi Pier,

In INCA select the spectrum in any of the Navigator
windows (Acquire, Confirm, Report etc.), right mouse
click, then select 'Export'. You have the choice of image
and file types inculding EMSA format.

Ron

On Wed, 3 Oct 2001 19:19:49 +0200 FABBRI
{fabbri-at-cigssrv1.unimo.it} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Does anyone give me informations about Oxford INCA 100 EDS file format?
} Any reference for an INCA {--} EMS spectra converter?
}
} P.L. Fabbri
}
} ---------------------------------------------
} Dr. Pier Luigi Fabbri
} C.I.G.S. - Centro Interdip. Grandi Strumenti
} Università di Modena
} via Campi 213/A - 41100 Modena, Italy
} Tel +39-059-2055231 / +39-059-370551
} Fax +39-059-370551
} E-mail: fabbri-at-mail.cigs.unimo.it
} ---------------------------------------------
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Oct 4 07:57:01 2001



From: Mayer, Helen K :      Helen.Mayer-at-UCAR.com
Date: Thu, 4 Oct 2001 08:46:03 -0400
Subject: East coast repair for B&L scope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been very happy with Paul Musci of M&R Optical. He is a former B&L
serviceman. He can be reached at:

PO Box 515
Charlton City, Massachusetts 01508
508-248-4658

Helen Mayer
UCAR Carbon
Parma, OH

-----Original Message-----
} From: "David_R_Stadden-at-armstrong.com"-at-sparc5.microscopy.com
[mailto:"David_R_Stadden-at-armstrong.com"-at-sparc5.microscopy.com]
Sent: Wednesday, October 03, 2001 12:02 PM
To: Microscopy-at-sparc5.microscopy.com




Anyone know of a good repair facility in PA or close by for a Bausch & Lomb
Stereozoom 7? I'm told the focusing rack is messed up. Appreciate any
leads.

Dave Stadden
Armstrong World Industries, Inc.
717-396-5109
drstadden-at-armstrong.com




From daemon Thu Oct 4 10:05:07 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 04 Oct 2001 09:40:17 -0500
Subject: Re: Microwave workshop

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We still have a few spots available for our Microwave Specimen preparation workshop. The information follows. Please let me know immediately if you would like to attend.
Thanks, Debby

===========================

The workshop will be held October 21, 22, and 23 in the Life Science Microscopy Facility at Purdue University, Wst Lafayette, Indiana, and run by Richard Giberson of Ted Pella, Inc.

Sunday Oct 21: introductory session starting at 2:00pm
Monday Oct 22: Hands-on prep for TEM and SEM
Tuesday Oct 23: Hands-on prep for ICC including block prep and ICC protocol.

We will have two microwave ovens, 2 TEM's, 1 SEM and 4 microtomes available during this time so there should be plenty of opportunity for examining the final products.

Cost for the workshop will be $350. This registration cost will include the book " Microwave Techniques and Protocols" by Giberson and Demaree, meals from dinner on Oct 21 through lunch on Oct 23, and most materials. Participants will have to provide their own resins if using ones other than Epon generic, Spurr's, or LR White. Also participants will have to provide their own specimen material (call and we will try to obtain it here if possible) and diamond knives for microtoming.
Check with us if you need any other special reagents to determine if we can provide them.

The workshop will be limited to 12 participants. Let me know immediately if you would like to register and I will send info on where to send the registration checks. Please note that once you have registered the registration costs can only be refunded if we can fill the spot. All fees will be refunded if the workshop must be cancelled for any reason.

Participants will also have to provide their own transportation and hotel rooms. We have a block of rooms reserved at the University Inn. The Purdue rate is $70 for 1 person and $77/room for 2 people. We will be happy to try to match up participants who would like to share a room. Contact me for reservation information.

Those who plan to drive in can contact me for instructions. West lafayette is reached via I-65 from Indianapolis (~1 1/4 hr northwest) or Chicago (2+ hr. southeast) and via I-74 from central Illinois (~1 1/2h northwest from Champagne-Urbana).

Those who plan to fly can reach us by flying into Indianapolis and taking ground transportation to campus (~ 1 1/2 hr.. and $20 one-way). Northwest Airlines has commuter flights directly into the Lafayette/Purdue airport from Detroit. Note that we stay on Eastern Standard time all year round so are on Central time in the summer and Eastern time in the winter (at least we don't have to remember to change our clocks!). Planes presently fly into Lafayette leaving Detroit at 5:10 Saturday nights. There are only evening flights coming in on Sunday. Flights to Detroit leave at 6:01 in the evening or 7:15 in the morning during the week.


Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-099e Whistler Building
West Lafayette, IN 47907


On Wednesday, September 26, 2001 8:48 AM, Tindall, Randy D. {TindallR-at-missouri.edu} wrote:
} Hi Debby,
}
} I'd like to receive information about this workshop. (Lou Ross forwarded
} your message to me.) I doubt if I can get funding to make it, but I may
} try.
}
} Thanks,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}



From daemon Thu Oct 4 10:32:39 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Thu, 4 Oct 2001 10:28:00 -0500
Subject: MAS Membership Renewal

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Hi,

The Microbeam Analysis Society (not MSA) membership renewal packets were
mailed out late last week. Inside the packet you will find a membership
renewal form, a M&M Journal subscription form and a ballot for 2002
Executive Council positions. There is a deadline for the 2002 M&M Journal
subscription of December 15, 2001 and the ballots must be postmarked by
December 31, 2001. Everything can be returned in the envelope provided.
Please take a few minutes to fill out the forms and ballot and drop them in
the mail as soon as possible. This will help to avoid any future problems.

If you have not received your packet in the next few days, have any
questions, or if you are not a member of the Microbeam Analysis Society and
would like to join, please contact me at the email addresses or phone
numbers below.

Thank you for your co-operation.
Lou Ross
MAS Membership Services
masmembership-at-excite.com
1-800-4MASMEM (1-800-462-7636)
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.bioterc.missouri.edu/emc


From daemon Thu Oct 4 11:10:53 2001



From: Sergei V. Kalinin :      sergei2-at-seas.upenn.edu
Date: Thu, 04 Oct 2001 12:02:48 -0400
Subject: TEM electron emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everybody
Can you recommend any references that might have total emitted
electron coefficient for different materials as a function of beam
energy? I am interested in oxides, particularly in materials like PZT
and BaTiO3.
Thanks in advance
Sergei



--
Sergei V. Kalinin
Dept. Mat. Sci. Eng.
University of Pennsylvania,
3231 Walnut St, Philadelphia PA 19104
Tel: (215) 898-3446
Fax: (215) 573-2128
E-mail: sergei2-at-seas.upenn.edu
URL: http://www.seas.upenn.edu/~sergei2




From daemon Thu Oct 4 17:56:05 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 4 Oct 2001 17:46:21 -0500
Subject: EM: MT2B microtome servicing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleage of mine is inquiring if anyone knows of a person who can
do routine servicing of MT2-B
Porter Bloom microtomes in Central States area (central Illinois, St.
Louis, Mo).

The only name they have is Bill McGee from New York. They are
worried that getting someone from NY area would be very costly.

Respond directly to Dr. Chang at: Chang.Nada-at-uis.edu

OR, I will forward any responses.

Thank you.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Thu Oct 4 19:11:46 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Thu, 4 Oct 2001 19:06:00 -0500
Subject: IMPORTANT follow-up to MAS Membership Renewal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I made a drastic mistake in my earlier email announcement. Ballots must be
postmarked by DECEMBER 1, 2001 to be counted. I apologize for the wrong
date. All the more incentive to return your application, subscription and
ballot as soon as possible.

Thanks,
Lou Ross
MAS Membership Services
masmembership-at-excite.com
1-800-4MASMEM (1-800-462-7636)
www.microanalysis.org

Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
web: www.biotech.missouri.edu/emc


From daemon Fri Oct 5 09:53:09 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Fri, 05 Oct 2001 10:38:31 -0400
Subject: Food Structure and Functionality Symposium 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Food Structure & Functionality Symposium 2002
May 5 to 8, 2002, Palais des Congres de Montreal, Montreal, Quebec, Canada.

An international symposium leading Food Structure & Functionality studies through the 21st century
"webaddress at the AOCS site (http://www.aocs.org/member/division/fsff/index.htm)"
Held in conjunction with the 93nd AOCS Annual Meeting & Expo (www.aocs.org)

The mandate of the Food Structure & Functionality Forum : "To promote global
collaboration between Food and Agriculture professionals in Structure and Functionality
disciplines by facilitating and providing a forum for exchange of knowledge, expertise and
research findings".

The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and
including trouble shooting.

Tentative Program Schedule
Sunday, May 5th
Short Course - Understanding structure-function relationships in food systems through specific localisation methods
and microscopy. Contact: Marcel Paques (Paques-at-nizo.nl)

Monday, May 6th
Opening of symposium
Plenary Speaker
Dairy Applications Session. Chairs: Mark Auty (mauty-at-moorepark.teagasc.ie) and Harjinder Singh (H.Singh-at-massey.ac.nz)
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl) and David Pechak (Dpechak-at-kraft.com)

Dedicated Poster Session
Division Board Meeting

Tuesday, May 7th
Agricultural Applications of Microscopy and Imaging Session./ joint with Feed Microscopy Division. Topic/Tentative title: New Microscopic Techniques for Identifying Food/Feed Constituents and Contaminants.
contacts: Mark Auty (mauty-at-moorepark.teagasc.ie) and Kim Koch
Division Luncheon and round table (expert) discussion. Topic TBA
Microbiology and Food Session. Chairs Judy Arnold (jarnold-at-saa.ars.usda.gov) and Ida Yates (iyates-at-ars.usda.gov)

Division Members Meeting

Wednesday, May 8th
Ingredients and Food Processing Session. Chairs: Diana Kittleson (dkittleson-at-pillsbury.com) and Bernhard Tauscher (bernhard.tauscher-at-bfe.uni-karlsruhe.de)

New Methods and Techniques for Food Structure and Functionality Analysis Session.
Chairs: Kathy Groves (Kgroves-at-LFRA.co.uk) and Maud Langton (maud.langton-at-sik.se)

Closure of Symposium.

¯------------------------------------------------------------------------------------------------------------------
For more information visit the websites mentioned above, contact any of the session chairs listed above or myself.

Paula Allan Wojtas,
Chair, Food Structure and Functionality Forum - a Division of AOCS

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Fri Oct 5 10:13:11 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Fri, 5 Oct 2001 10:59:41 -0700
Subject: mark rigler?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We are trying to locate Mark Rigler of Materials Analytical Services. Have
they gone out of business? Their 800# says "all circuits are busy now"...it
has been like that for days.
thanks for your help,
Beth

******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************




From daemon Fri Oct 5 12:37:18 2001



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Fri, 5 Oct 2001 18:55:59 +0100 (CAT)
Subject: EDX and SEM coater

Contents Retrieved from Microscopy Listserver Archives
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Hi everybody,
I am the operator of the SEM for my university and I am trying to shop for
these two accesorries
1. Coater
2. EDX accesories
The SEM is a LEO 1400.
Soji




From daemon Fri Oct 5 14:57:33 2001



From: coviello :      coviello-at-mae.uta.edu
Date: Fri, 05 Oct 2001 02:47:10 -0500
Subject: TEM-HRTEM samples with Gatan 600 ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
I am looking for suggestions from experienced microscopists for voltage
and current settings for the final cleaning to remove ion milling damage
on Silicon and GaAs samples for High Resolution microscopy using a Gatan
600 ion mill. Presently we use the standard 5Kv, 0.5 milliamps/gun with
"splotchy" and/or inconsistent results ( we are trying "tweak" our
settings in order to reduce/eliminate the amorphous regions).
Thanks in advance,
Mike
UT Arlington



From daemon Fri Oct 5 16:33:22 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 5 Oct 2001 17:09:24 -0400
Subject: TEM-HRTEM samples with Gatan 600 ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The lowest you can go on the Duomill is about 10 degrees on the top side (with no top plate) and 12 degrees on the bottom side. With normal guns, you can go to about 2.5 - 3 kV with 2.5 mA.

If you insist on ion milling and you can only use the duomill, you can buy the PIPS sample holders and a pedestal from Gatan that can be used in the duomill to go to low angles. The lowest angles on dimple samples will be about 4 degrees on the dimpled side (assuming single sided dimpling). This should help you quite a bit. You will not have a lot of beam current to work with, but it should give you what you want with a little experimentation.

Now if you want no amorphous areas at all, (and I do mean none) you should use the small angle cleavage technique. Check out the Microcleave(TM) Kit from South Bay Technology on their web site. They have a couple of my PDF files that illustrate the quality of the samples that you can get.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: coviello [mailto:coviello-at-mae.uta.edu]
Sent: Friday, October 05, 2001 3:47 AM
To: listserver


Hi All:
I am looking for suggestions from experienced microscopists for voltage
and current settings for the final cleaning to remove ion milling damage
on Silicon and GaAs samples for High Resolution microscopy using a Gatan
600 ion mill. Presently we use the standard 5Kv, 0.5 milliamps/gun with
"splotchy" and/or inconsistent results ( we are trying "tweak" our
settings in order to reduce/eliminate the amorphous regions).
Thanks in advance,
Mike
UT Arlington



From daemon Fri Oct 5 16:56:01 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sat, 6 Oct 2001 09:47:21 GMT+1200
Subject: Vacation Support

Contents Retrieved from Microscopy Listserver Archives
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Hi, friends

I look after a JEOL 840 with EDS and 3 x WDS in a small department.

I usually put it in standby and there's no useage while I'm away, but
I'm being urged to train a deputy to mind it and help/supervise
users.

But when I think about the possibility of returning to find the
column and/or the spectros full of oil, or some other nightmare
scenario that I (thankfully) haven't yet envisaged, I become
apprehensive.

How do other places/people handle the problem of absences of the
primary/sole caregiver?

thanks

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Fri Oct 5 17:55:00 2001



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Fri, 5 Oct 2001 17:41:57 -0500
Subject: Schiff Reagent for Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello fellow histo/micro netters,

Does anyone have any information on Schiff's reagent that contains thionine
or any other blue stain. If so who is the manufacturer, who is the
distributor, what's the cost, what's the shelf life, etc., etc.. I am
looking for an alternative Feulgen stain for DNA ploidy analysis via Image
Analysis.

Thank you in advance.



Donald G. Awbrey, HT(ASCP) QIHC
Image Analysis / Electron Microscopy
donaldawbrey-at-texashealth.org
donaldawbrey-at-hotmail.com



From daemon Sat Oct 6 02:05:42 2001



From: Earl Weltmer :      eweltmer-at-home.com
Date: Sat, 6 Oct 2001 05:31:40 -0700
Subject: High Voltage Cable Repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We pray.

Earl

----- Original Message -----
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, October 06, 2001 2:47 AM


Dear Listers,

I have a JEOL 35C with a defective high voltage cable. It appears to be
"open" at the feedthrough to the gun.
As the SEM is quite old JEOL no longer supplies replacement cables.

Does anyone know a source to repair this cable?

Thank You,

Earl Weltmer



From daemon Sat Oct 6 07:48:33 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Sat, 6 Oct 2001 08:41:25 -0400
Subject: RE: Schiff Reagent for Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Don,
But the Feulgen Schiff has been proved to be stoichiometric! There
are alternatives, but you would have to prepare them yourself and insure
that the stoichiometry holds, but, then, I have always made my own Schiff
reagent anyway. (Method on request, but it is readily available.) [A.G.E.
Pearse, Histochemistry, Theoretical and Applied, (4th Ed.), Churchill
Livingstone, New York, 1985, Vol II, ISBN: 0-443-02997-0] always has
plentiful methods, and the most recent edition (Vol II) not only has many
methods [including alternatives to Schiff!] but a DEEP discussion of the
entire Feulgen-DNA methodology and history.
Hope this helps.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/




} ----------
} From: Awbrey, Donald
} Sent: Friday, October 5, 2001 6:41 PM
} To: 'microscopy-at-msa.microscopy.com'
} Subject: Schiff Reagent for Image Analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hello fellow histo/micro netters,
}
} Does anyone have any information on Schiff's reagent that contains
} thionine
} or any other blue stain. If so who is the manufacturer, who is the
} distributor, what's the cost, what's the shelf life, etc., etc.. I am
} looking for an alternative Feulgen stain for DNA ploidy analysis via Image
} Analysis.
}
} Thank you in advance.
}
}
}
} Donald G. Awbrey, HT(ASCP) QIHC
} Image Analysis / Electron Microscopy
} donaldawbrey-at-texashealth.org
} donaldawbrey-at-hotmail.com
}
}
}


From daemon Sat Oct 6 11:04:45 2001



From: Jon Ekman :      ekman-at-bio.fsu.edu
Date: Sat, 6 Oct 2001 10:50:03 -0500
Subject: DVD RAM media help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

We recently purchased a DVD RAM drive to assist with image storage.
The problem is that our IT department and local venders don't know
much about the media (The actual disks). We have two choices for DVD
RAM disks: authoring or general use. Has anyone out there figured
out which is the best for general microscopy images. The disks will
travel between Mac's, PC's and LINUX/UNIX operating systems.

TIA,

Jon Ekman
Florida State University
Biological Science Imaging Resource
119 Bio Unit I, 4370
Tallahassee, FL 32306
tel: 850.644.6519
fax: 850.644.0481


From daemon Sat Oct 6 11:15:42 2001



From: Jim Bentley :      bentleyj-at-ornl.gov
Date: Sat, 6 Oct 2001 11:09:46 -0500
Subject: MSA Student Travel Awards, ICEM-15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



John H. L. Watson Memorial Student Scholarships
15th International Congress on Electron Microscopy (ICEM-15), Durban,
South Africa, Sep 1-6, 2002

In memory of John H. L. Watson, the Microscopy Society of America
(MSA) has established a Travel Scholarship Award to encourage
graduate students and post-doctoral research assistants who are MSA
members in good standing, to attend international microscopy
meetings. (Note: In the future, The John L. Watson Memorial Student
Scholarships should also be available to enable young researchers
from Latin America and South America to attend appropriate
international meetings hosted by the MSA in the USA).

The MSA will pay from $500 to $1250 towards travel and accommodation
to allow graduate students or post-doctoral research assistants (with
Doctoral degrees awarded on or after January 1, 1998) who are members
of the MSA to attend the 15th International Congress on Electron
Microscopy (ICEM-15), in Durban, South Africa, September 1-6, 2002.
The level of the monetary award will be based on the scientific
content of the paper to be presented and on the number of successful
applicants.

Applicants should forward to the MSA Awards Committee Chair, Dr. J.
Bentley, MS-6136 4500-S, Oak Ridge National Laboratory, 1 Bethel
Valley Rd, Oak Ridge, TN 37831-6136:
(i) their full name, age, address and details of academic and/or
professional status,
(ii) a letter of support from their advisor/supervisor that also
attests to the eligibility of the applicant, and
(iii) an "advanced" copy of their 2-page paper/extended abstract
intended for submission to ICEM-15, on which the applicant is the
lead author.

The deadline for submission is December 15, 2001. This date will
allow time for evaluation of the paper by the MSA Awards Committee,
confirmation with the MSA Business Office that the applicant is a
member in good standing and has renewed their dues for 2002,
selection/approval by MSA President, and notification of successful
and unsuccessful applicants in time to meet the submission deadline
of papers to ICEM-15 (February 1, 2002). Successful applicants will
be required to submit an appropriate paper/extended abstract in
sufficient time and of sufficient scientific content to be published
in the ICEM-15 proceedings. Unsuccessful applicants will be
encouraged to submit an abstract to ICEM-15, but will receive no
financial support from this award.

__________________________________________________________________________
Jim Bentley
Microscopy Microanalysis Microstructures Group
Metals and Ceramics Division
Bldg. 4500-S, MS-6136
Oak Ridge National Laboratory
PO Box 2008
Oak Ridge, TN 37831-6136, USA

Tel: (865)574-5067 Fax: (865)241-3650 bentleyj-at-ornl.gov
express mail use "1 Bethel Valley Rd" instead of PO Box.
** Note the new group name, mail-stop, fax, and area code **


From daemon Sat Oct 6 15:32:43 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 06 Oct 2001 13:29:00 -0700
Subject: Re: DVD RAM media help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


General use media is all that you need. There are two types,
Type 1 5.2GB and Type 2 2.6GB. Type 1 is double sided and
can only be used in its container. Type 2 can be used in the
container or removed for reading in DVD-ROM drives.

Check your drive and media carefully. I've used Panasonic
and Pioneer drives, TDK and Maxell media. When writing
data to the media, I had no problem. When reading the
data from the media, many files were corrupted. Any file
larger than about 1MB was likely to be damaged. Be sure
to perform a verify after writing. Routine scandisk is also
a good idea.

I don't use DVD-RAM any more since I cannot trust them.
I switched to DVD-R and DVD-RW. No problems with this
product.

gary g.


At 08:50 AM 10/6/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Oct 7 19:07:21 2001



From: Lachlan McDonald :      lmcdonald-at-engeneic.com
Date: Mon, 8 Oct 2001 09:57:07 +1000
Subject: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello All,

I'm particularly interested in an electron microscopy application, hope
someone out there can help or point me in the right direction.

What I want to do is follow the route of a phagocytosed bacterium from the
cell surface attaching initially to the cell surface or pseudopods to being
engulfed right through to it's final destination within a lysosome and all
the intervening steps in between. I have a very good antibody to a cell
surface antigen on the bacteria which has been shown to work well in an
immunogold labelling previously so I'd like to try and repeat this.

As I have no experience with TEM or indeed preparation of the samples I
would appreciate very much any useful comments or protocols on this matter.
I intend to grow the cells in flasks then add the bacteria and take a
variety of time points to catch the range of stages of the bacteria
through the cell - is there perhaps a better way to setup this experiment ?
How might I collect the sampes, ie cell scraping or limited ezymatic
digestion to get the cells of the cell culture plat ? I really want to
preserve cell morphology as much as possible and the show the very early
contacts between macrophage & bacterium at the cell surface so am concerned
about the best way to harvest the cells, ie enzymatic harvseting or cell
scraping may be worrysome. Is it possibe to process the cells for TEM &
immunogold analysis whilst still attached to the plastic of a cell culture
flask (ie break the flask open and somehow embed/ process the cells whilst
still in-situ ?).

Any useful comments would be greatly appreciated !

Many thanks in advance.

Lachlan



From daemon Mon Oct 8 04:49:24 2001



From: Steve Chapman :      protrain-at-emcourses.com
Date: Mon, 8 Oct 2001 10:36:00 +0100
Subject: Re: High Voltage Cable Repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

In Europe when I was hopping from country to country I found that the
organisations that work with X-ray sets in hospitals etc are more than
capable of fixing a high voltage cable. We have even replaced the complete
cable simply taking the two ends from the EM cable.

Hope this helps?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com




From daemon Mon Oct 8 12:17:30 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Mon, 08 Oct 2001 10:05:19 -0700
Subject: Re: TEM-HRTEM samples with Gatan 600 ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mike:

You bring up a very good issue of removing amorphous damage from
specimens which has become of increasing interest in HREM applications.
Removing amorphous damage from specimens has been most recently
addressed with the advent of low energy ion milling. This is a
technique where the final milling stage is done at ~ 100-500eV. This is
based on technology developed by Dr. Arpad Barna in Budapest and has
shown some tremendous results. We market the aptly named "Gentle Mill"
and you can find results for both silicon and GaAs on our website at
www.southbaytech.com. Go to the search function and enter "gentle" and
that will bring you to the right page. You need to register to download
the PDF - if that's a bother, just send me an email and I will email the
file to you.

I am sorry for the somewhat commercial nature of the response, but I do
think it appropriately addresses the issue. I hope it helps.

Best regards-

David

"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} The lowest you can go on the Duomill is about 10 degrees on the top side (with no top plate) and 12 degrees on the bottom side. With normal guns, you can go to about 2.5 - 3 kV with 2.5 mA.
}
} If you insist on ion milling and you can only use the duomill, you can buy the PIPS sample holders and a pedestal from Gatan that can be used in the duomill to go to low angles. The lowest angles on dimple samples will be about 4 degrees on the dimpled side (assuming single sided dimpling). This should help you quite a bit. You will not have a lot of beam current to work with, but it should give you what you want with a little experimentation.
}
} Now if you want no amorphous areas at all, (and I do mean none) you should use the small angle cleavage technique. Check out the Microcleave(TM) Kit from South Bay Technology on their web site. They have a couple of my PDF files that illustrate the quality of the samples that you can get.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
} -----Original Message-----
} } From: coviello [mailto:coviello-at-mae.uta.edu]
} Sent: Friday, October 05, 2001 3:47 AM
} To: listserver
} Subject: TEM-HRTEM samples with Gatan 600 ion mill
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All:
} I am looking for suggestions from experienced microscopists for voltage
} and current settings for the final cleaning to remove ion milling damage
} on Silicon and GaAs samples for High Resolution microscopy using a Gatan
} 600 ion mill. Presently we use the standard 5Kv, 0.5 milliamps/gun with
} "splotchy" and/or inconsistent results ( we are trying "tweak" our
} settings in order to reduce/eliminate the amorphous regions).
} Thanks in advance,
} Mike
} UT Arlington

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


From daemon Mon Oct 8 16:11:45 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 08 Oct 2001 14:04:10 -0700
Subject: Re: DVD RAM media help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are using magneto-optical disks (MO-disk) for 5+ years. Since that time
we had 1 disk damaged (and successfully recovered) out of few hundreds
which is in circulation currently. Each disk is 2.6 or 5.2 Gb and
9-something available now. The disk uses FAT or NTFS format. DVD format
is not established well and a subject of frequent changes over last few
years, so I would avoid it unless they establish standard and will follow
it. "FAT"- disks are readable from any platform and no software necessary
to operate the drive (it's SCSII) - it's visible on desktop immediately
after connection.

Sergey

At 01:29 PM 10/6/01 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Mon Oct 8 19:29:34 2001



From: Richard Easingwood :      richard.easingwood-at-stonebow.otago.ac.nz
Date: Tue, 9 Oct 2001 13:29:18 +1300
Subject: TEM: EM Stainer experiences?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We recently purchased a new Leica EM Stainer to replace our aging (but
otherwise pretty trusty) LKB Ultrostainer and have some misgivings about
our new acquisition. Until the company has had a bit longer to try to sort
the problems I do not want to go into specifics here but despite of
spending several months trying to sort out theses 'issues' we are a long
way from being happy with the instrument - this is despite it having been
back to the factory once.

I would really like to know if other owners and users of the new EM Stain
have experienced major problems with this piece of equipment - or if its
just our one (or us!). If your EM Stain has proved totally satisfactory,
I'd like to hear from you too...

Best regards,


Richard

Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: office: 0064 3 479 7301
Facsimile: 0064 3 479 7254
GSM: 0064 21 222 4759

mailto:richard.easingwood-at-stonebow.otago.ac.nz
Web site: http://www.otago.ac.nz/anatomy/emunit/








From daemon Tue Oct 9 08:09:47 2001



From: McFaddin, Wade :      Wade.McFaddin-at-nextekinc.com
Date: Tue, 9 Oct 2001 07:59:24 -0500
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nextek Inc. is seeking a qualified individual to join our analytical
laboratory team as a Materials/Failure Analysis Engineer. The ideal
candidate will have a B.S. in Materials Science, Chemistry, Engineering or
equivalent, and a minimum 3 years experience in the micro-electronic field;
or 5 or more years hands-on lab experience in the micro-electronic
materials/failure analysis
field. Knowledge in cross-sectional and destructive analysis of
micro-electronic assemblies and devices, and experience with analytical
instrumentation such as; SEM/EDS, Micro-FTIR, Thermal Analysis, Acoustic
and Optical Microscopy is a plus.

To learn more about Nextek Inc. visit our web site at
http://www.nextekinc.com/. For more
information about this position, call or email the following:

Wade McFaddin (256) 772-1995 ext.1064
wade.mcfaddin-at-nextekinc.com
or
Jim Chiang (256) 772-1995 ext. 1029
james.chiang-at-nextekinc.com




From daemon Tue Oct 9 09:16:26 2001



From: Hong Yi :      hyi-at-emory.edu
Date: Tue, 09 Oct 2001 10:36:49 -0400
Subject: Antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:
I am looking for a monoclonal anti pancreatic amylase antibody. Does
anyone out there know a source. Thank you in advance.

Hong
------------
Hong Yi
Emory Neurology Microscopy Core Laboratory
Emory University Department of Neurology
6215 Woodruff Memorial Research Building
1639 Pierce Dr.
Atlanta, GA 30322
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu



From daemon Tue Oct 9 10:13:25 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 09 Oct 2001 10:59:08 -0400
Subject: Beta Probe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Listers!

Many years ago, when (or even before) I was an undergraduate (that would
place it in the early '60s), my aunt managed the analytical lab at a
company that produced non-ferrous metals (principally, I think, bronzes and
brasses). As one does in such situations, she invited me to tour her labs
one day.

One of the instruments I was shown was called a "Beta Probe". A piece of
the metal was ground and polished, and was then placed face down on an
aperture in the top plate, with what I now realize was an O-ring seal. A
few buttons were pressed, and after a little while some of the electronic
counters of the day (neon tubes with 10 electrodes) began spinning round.
My memory is that the operator had some range of ratios between the
recorded numbers within which he (it was, of course, a "he" in those days,
even though my aunt was the manager) could pass the sample as meeting
specifications.

I surmise that this was an electron milli-probe (if you'll forgive my
coining of the term!) with preset spectrometers tuned to the elements of
interest in the specification. I would very much like to find out more
about this device. Happily, my aunt is still available to be asked, but
she knows nothing of the operation of the instrument. The people who ran
it have either died, or lost touch (the company failed twenty years ago).

If anyone can give me more information about this instrument, I would be
very interested, and most grateful.

Thanks,

Tony


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*




From daemon Tue Oct 9 10:19:52 2001



From: Nancy Cherim :      nac-at-cisunix.unh.edu
Date: Tue, 9 Oct 2001 03:52:23 -0400
Subject: Position Announcement: Director of University of New Hampshire's Instrumentation Center

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The University of New Hampshire is looking for a Director of the
Instrumentation Center. For more information please go to our website at:

www.unh.edu/instrumentation-center/index

Analytical services at the UIC include: Elemental Analysis; Gas
Chromatography-Mass Spectrometry (GC-MS); High-Field Nuclear Magnetic
Resonance (FTNMR - 90, 360, 400, and 500 MHz); Ultra-Violet, Visible, and
Near-Infrared Spectrophotometry (UV-Vis-NIR); Infrared Spectroscopy (FTIR);
Optical Rotation; X-Ray Photoelectron Spectroscopy; Electron Microscopy
including Scanning Electron Microscopy (SEM) and Transmission Electron
Microscopy (TEM) with Energy Dispersive Spectroscopy (EDS).

RESPONSIBILITIES: Reporting to the Vice President for Research and Public
Service, the University Instrumentation Center Director will be responsible
for leading an emerging research and teaching oriented service center toward
excellence; duties to include:

Working directly with faculty, administrators, and clients from diverse
backgrounds and perspectives to maximize use of all facilities within the
UIC; forging partnerships with researchers, teaching oriented faculty
members, other departments, programs, and research centers and institutes
for purposes of proactively supporting research and instruction;

Managing the financial, administrative, and supervisory aspects of the UIC;

Supporting education and training of staff, university researchers/clients,
and students;

Securing funding through external and internal sources in order to obtain
new instruments and upgrade existing equipment;

Promoting UNH outreach mission by developing knowledge and awareness of
other laboratories in the state and region, for purposes of forming
partnerships, referral relationships, information networks, etc. Serving as
a resource for state government and business and industry.

QUALIFICATIONS:

Advanced degree in physical sciences or engineering.

Minimum of three years managerial experience.

Demonstrated experience with operation of analytical instruments and
application to research problems.

Familiarity with securing external funding for scientific instruments.

Excellent oral and written communication skills.

Demonstrated ability to work with a variety of constituents.

STARTING DATE: Negotiable, as early as 12-1-01

SALARY: Competitive and commensurate with experience and local area.

APPLICATION DEADLINE: Open until filled.

APPLICATION PROCEDURE: Applicants should send a letter of intent, CV/resume,
and the names, addresses, and phone numbers of three references to:

Chair, University Instrumentation Center Director Search Committee

C/o Office of the Vice President for Research and Public Service

Room 107, Thompson Hall

University of New Hampshire

Durham, NH 03824

Electronic resumes and supporting documentation will be accepted. Please
e-mail your package to: diana.couture-at-unh.edu





From daemon Tue Oct 9 11:39:36 2001



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 09 Oct 2001 09:29:53 -0700
Subject: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone offer any ideas on the best way to proceed with removing the
effects of charging on an image? I am referring to the streak across the
image manifested as a change in density. As an example, have a look at
this uncoated mite:

http://katie.ucdavis.edu/pics/sucker2.jpg

The nature of this projects requires that we place the live mite in the
scope and get a picture in the first 5 minutes. This picture was taken on
a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
for publication. I am looking for some help in eliminating some of the
charging artifacts without losing too much detail. Any help will be
appreciated.

We are using PS 6 and I have the IPTK plugins. I made some progress using
a Gaussian filter to produce a very fuzzy duplicate image, invert the
image, add it to a layer and then adjust the opacity to around 50%. This
was only partially successful. Any help will be appreciated.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue Oct 9 12:36:59 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 09 Oct 2001 12:30:32 -0500
Subject: Re: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be interested to hear of any IP solution you come up with.

However, I would think that running the scope in environmental mode at
higher voltage ought to be able to give you a satisfactory image.

We have a Hitachi S2460N here. We routinely run 40 Pa of Helium to
eliminate charging. At more than 10 kV, we can get a suitable image off of
our Robinson BSE. Our Oxford Tetra(tm) allows us to drop the voltage
further. I will forward you an image of a bug from our scope under separate
cover.

Warren

At 09:29 AM 10/9/2001 -0700, you wrote:

} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image? I am referring to the streak across the
} image manifested as a change in density. As an example, have a look at
} this uncoated mite:
}
} http://katie.ucdavis.edu/pics/sucker2.jpg
}
} The nature of this projects requires that we place the live mite in the
} scope and get a picture in the first 5 minutes. This picture was taken on
} a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
} for publication. I am looking for some help in eliminating some of the
} charging artifacts without losing too much detail. Any help will be
} appreciated.
}
} We are using PS 6 and I have the IPTK plugins. I made some progress using
} a Gaussian filter to produce a very fuzzy duplicate image, invert the
} image, add it to a layer and then adjust the opacity to around 50%. This
} was only partially successful. Any help will be appreciated.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Tue Oct 9 14:11:24 2001



From: Privileged Information :      21stnetwork-at-mailandnews.com
Date: Tue, 9 Oct 2001 14:07:19 -0500 (CDT)
Subject: The Power Group

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From daemon Tue Oct 9 14:41:56 2001



From: DrJohnRuss-at-aol.com
Date: Tue, 9 Oct 2001 15:35:35 EDT
Subject: Re: Photoshop and SEM charging

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In a message dated 10/9/01 12:45:01 PM, raharris-at-ucdavis.edu writes:

} We are using PS 6 and I have the IPTK plugins. I made some progress using
} a Gaussian filter to produce a very fuzzy duplicate image, invert the
} image, add it to a layer and then adjust the opacity to around 50%. This
} was only partially successful. Any help will be appreciated.

Another approach you might try is to duplicate the image, smooth the copy
with a Gaussian blur, and then divide by it. The size of the blur to use
depends on the size of the bright streaks, and these are not consistent in
your image, but values in the range of 2-4 pixels seem to work fairly well.


From daemon Tue Oct 9 16:16:33 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Tue, 09 Oct 2001 14:09:10 -0700
Subject: RE: Photoshop and SEM charging

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Hi Rick,
If the image is saturated with white pixels ("blown out highlights")
then your are pretty stuck. There isn't anything that can be done to put
information in where it doesn't exist. However, in order to make the most out
of a bad situation, you can use the levels and curves options to adjust the
brightness and contrast for a reasonable image. I prefer to use adjustment
layers to do this, that way I can edit and tweak the settings without
permanently changing the core data.
Your best best is to avoid/minimize saturation when you capture your
images. A "wet" ESEM is fantastic for this. As you probably know, try to
minimize spot size, current, and voltage to minimize charging artifacts, and
choose brightness and contrast settings that don't saturate the image.
If you want to see what I mean by adjustment layers using curves and
levels, you can send me a file, and I'll set it up for you, and send it back for
you to look at. Good luck.

-Brad

----------
From: Rick Harris
Sent: Tuesday, October 9, 2001 9:29 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Photoshop and SEM charging

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Can anyone offer any ideas on the best way to proceed with removing the
effects of charging on an image? I am referring to the streak across
the
image manifested as a change in density. As an example, have a look at
this uncoated mite:

http://katie.ucdavis.edu/pics/sucker2.jpg

The nature of this projects requires that we place the live mite in the
scope and get a picture in the first 5 minutes. This picture was taken
on
a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor

for publication. I am looking for some help in eliminating some of the
charging artifacts without losing too much detail. Any help will be
appreciated.

We are using PS 6 and I have the IPTK plugins. I made some progress
using
a Gaussian filter to produce a very fuzzy duplicate image, invert the
image, add it to a layer and then adjust the opacity to around 50%.
This
was only partially successful. Any help will be appreciated.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu





From daemon Tue Oct 9 17:59:31 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 09 Oct 2001 18:54:53 -0400
Subject: Re: Photoshop and SEM charging

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Rick,
My experience is that live insects don't generally charge because the
evaporating water carries the charge away. From the looks of the image,
I think you were using a lot of beam current. Cur that back a whole lot
and the charging will probably go away. Also, the horizontal scan
speed has a much greater effect on charging than the vertical speed. If
you run a faster horizontal rate and more lines (slower vertical speed),
this will help.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Rick Harris wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone offer any ideas on the best way to proceed with removing
} the effects of charging on an image? I am referring to the streak
} across the image manifested as a change in density. As an example,
} have a look at this uncoated mite:
}
} http://katie.ucdavis.edu/pics/sucker2.jpg
}
} The nature of this projects requires that we place the live mite in
} the scope and get a picture in the first 5 minutes. This picture was
} taken on a Hitachi S3500N at 2.5 KV. The picture suits our needs but
} is too poor for publication. I am looking for some help in
} eliminating some of the charging artifacts without losing too much
} detail. Any help will be appreciated.
}
} We are using PS 6 and I have the IPTK plugins. I made some progress
} using a Gaussian filter to produce a very fuzzy duplicate image,
} invert the image, add it to a layer and then adjust the opacity to
} around 50%. This was only partially successful. Any help will be
} appreciated.
}
}
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}
}
}
}



From daemon Tue Oct 9 18:29:20 2001



From: Aruna Weberg :      AWEBERG-at-siumed.edu
Date: Tue, 9 Oct 2001 18:22:45 -0500
Subject: Nikon Objective

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Hi,

Our facility is looking to purchase a dry 1x objective for a Nikon Microphot
light microscope. I have contacted a few vendors and have been told that
the microscope is out of date and it will be impossible to locate such an
objective. Any help in locating this item will be appreciated.

Thank you in advance for your help

aruna


From daemon Tue Oct 9 18:49:37 2001



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Tue, 9 Oct 2001 16:44:12 -0700
Subject: Beta Probe

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Twin City International Inc. made a beta backscatter instrument called the
Betascope. It used an isotope source and a Geiger-Mueller tube to measure
the thickness of metal films after a simple calibration with known thickness
standards. I don't know if they are still in business.

-----Original Message-----
} From: Tony Garratt-Reed [mailto:tonygr-at-mit.edu]
Sent: Tuesday, October 09, 2001 7:59 AM
To: microscopy-at-sparc5.microscopy.com


Hi, Listers!

Many years ago, when (or even before) I was an undergraduate (that would
place it in the early '60s), my aunt managed the analytical lab at a
company that produced non-ferrous metals (principally, I think, bronzes and
brasses). As one does in such situations, she invited me to tour her labs
one day.

One of the instruments I was shown was called a "Beta Probe". A piece of
the metal was ground and polished, and was then placed face down on an
aperture in the top plate, with what I now realize was an O-ring seal. A
few buttons were pressed, and after a little while some of the electronic
counters of the day (neon tubes with 10 electrodes) began spinning round.
My memory is that the operator had some range of ratios between the
recorded numbers within which he (it was, of course, a "he" in those days,
even though my aunt was the manager) could pass the sample as meeting
specifications.

I surmise that this was an electron milli-probe (if you'll forgive my
coining of the term!) with preset spectrometers tuned to the elements of
interest in the specification. I would very much like to find out more
about this device. Happily, my aunt is still available to be asked, but
she knows nothing of the operation of the instrument. The people who ran
it have either died, or lost touch (the company failed twenty years ago).

If anyone can give me more information about this instrument, I would be
very interested, and most grateful.

Thanks,

Tony


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*





From daemon Tue Oct 9 21:43:45 2001



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au
Date: Wed, 10 Oct 2001 12:21:11 +1000
Subject: fluorescent cell wall dyes

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Hi,

Does anyone know of a dye that binds to cellulose (or pectins) and is
fluorescent? I am looking for an alternative to calcofluor. I wish to
stain 1 micron thick LR-white sections of Vicia faba cotyledons.

Any help would be greatly appreciated.

Mark Talbot.



From daemon Wed Oct 10 03:08:55 2001



From: JohnB :      jblinco-at-central.murdoch.edu.au
Date: Wed, 10 Oct 2001 16:04:10 +0800
Subject: LM: recommendations for digital camera

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Hi there,
I know this question has been asked lots, but i never needed to pay
any attention till now, so my apologies.
My institute wishes to purchase a very good digital camera for light
/ fluorescence microscopy to replace (compliment?) the 35 mm film
cameras that we currently use.
Part of the requirements is that the camera be able to be taken off
the scope and have a regular lens fitted for other high / professional
quality images (such as whole plants, animals etc.)
So the question I have to ask is, is this a reasonable task for a
system?
Further to that, what do current users of digital cameras for
microscopy see as an absolute requirement and what is nice to have?

A short list of cameras suggested to us locally are:
Canon D30 3.3 megapixel, Fuji/Nikon S1 3.3mp, Nikon DiX 5.5 mp.
Any comments on those (good or bad)? Any other recommendations?

John Blinco
Western Australian State Agricultural Biotechnology Center


From daemon Wed Oct 10 04:46:59 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Wed, 10 Oct 2001 09:14:18 -0300
Subject: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
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Mark
Congo red would be a suitable alternative. Excite with green and
view in orange/red - Rhodamine -type filter set.

You could also try a fluorescent pseudo-schiff reaction using
periodic acid or sodium m-periodate oxidation followed by staining
with a carbohydrazide derivative of a fluorescent dye - e.g. Lucifer
Yellow CH.

A dye suitable for uronic acid polysaccharides such as pectin is
Coriphosphine O CI No. 46020. Again this works with Rhodamine
type filter sets

Chris

Date sent: Wed, 10 Oct 2001 12:21:11 +1000
} From: MARK JEFFREY TALBOT {mark.talbot-at-studentmail.newcastle.edu.au}


The Hitachi S3500N has the N-SEM mode which should give you a reasonable
image in backscatter, with appropriate WD and pressure settings.
Also the 3500 has an integrate capture mode to do multiple rapid scans which
can help reduce charging.

David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford St.
Halifax, NS B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca
http://www.imb.nrc.ca/micros_e.html



-----Original Message-----
} From: Rick Harris [mailto:raharris-at-ucdavis.edu]
Sent: October 9, 2001 1:30 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone offer any ideas on the best way to proceed with removing the
effects of charging on an image? I am referring to the streak across the
image manifested as a change in density. As an example, have a look at
this uncoated mite:

http://katie.ucdavis.edu/pics/sucker2.jpg

The nature of this projects requires that we place the live mite in the
scope and get a picture in the first 5 minutes. This picture was taken on
a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
for publication. I am looking for some help in eliminating some of the
charging artifacts without losing too much detail. Any help will be
appreciated.

We are using PS 6 and I have the IPTK plugins. I made some progress using
a Gaussian filter to produce a very fuzzy duplicate image, invert the
image, add it to a layer and then adjust the opacity to around 50%. This
was only partially successful. Any help will be appreciated.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Wed Oct 10 08:12:45 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Wed, 10 Oct 2001 08:02:18 -0500
Subject: VPSEM vent gasses

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One of those answering Rick Harris' question on charging remarked
about using helium in the variable pressure mode. What other gasses
are used for the VPSEM and ESEM? What considerations are taken into
acccount in choosing a vent gas? Is there a paper somewhere that
answers these questions?

Chuck Butterick
Engineered Carbons, Inc.



From daemon Wed Oct 10 08:29:42 2001



From: Berg, R. Howard :      RHBerg-at-danforthcenter.org
Date: Wed, 10 Oct 2001 08:22:43 -0500
Subject: Re: fluorescent cell wall dyes

Contents Retrieved from Microscopy Listserver Archives
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We use Congo Red and image it in the rhodamine channel...



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Associate Member
Donald Danforth Plant Science Center/Nidus Center
893 North Warson
St. Louis, MO 63141

phone: 314-812-8076
fax: 314-812-8080
cell phone: 314-378-2409

NEW ADDRESS AS OF OCTOBER 15,2001:

Donald Danforth Plant Science Center
975 North Warson Road
St. Louis, MO 63132

http://www.danforthcenter.org


From daemon Wed Oct 10 08:32:39 2001



From: tbargar-at-unmc.edu
Date: Wed, 10 Oct 2001 08:19:05 -0500
Subject: TEM of PLGA nanoparticles and label

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Hi everyone,

One of our pharmacology researchers is making nanoparticles ranging in
sizes from 50nm up to 250nm. The material is
polylactic-acid-coglycolic-acid (PLGA). He's looking for a lipophilic
electron dense particle, dye or stain that could be incorporated into the
particles during their formation and which could be seen in the TEM
sections. The material has to be able to be suspended or dissolved in an
organic solvent.

For our part here are some of the things we've thought of using: Ruthenium
Red powder, colloidal gold in 5 to 10nm range, phosphotungstic acid in
ethanol.

His material will also melt at 45 degrees centigrade. So I embedded in
Unicryl, UV polymerization at 4 degrees centigrade. Does anyone know if
there is still heat produced during the polymerization?

This seems to be in the realm of polymer chemistry and materials science.
I'd appreciate any and all help, Thanks.

Tom Bargar
UNMC EM Lab
402-559-7347



From daemon Wed Oct 10 09:02:57 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Wed, 10 Oct 2001 23:56:44 +1000
Subject: Re: Photoshop and SEM charging

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We run a Hitachi S2250N. Rick, what gas, pressure and WD were you using? Helium is the best, as Warren says, but if you dont have a tank handy, water vapour (for a minimal system - just connect the gas inlet to a half-full flask of water!) is almost as good for imaging. And I would also suggest a higher voltage - 5kV minumum, 10-15 for comfort, if you want to work quickly at low magnifications and can afford to miss a bit of surface detail. On something with as many projections as a mite, utilising the VP system to eliminate charging will let you work much more rapidly than relying on low kV alone. Its not very PC, but we would tend to start at 20kV and work down....

regards
Sally Stowe




Dr Sally Stowe
Facility Coordinator,
ANU Electron Microscopy Unit
Research School of Biological Sciences
Australian National University
Canberra ACT0200
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6125 3218
ph 02 6125 2743
http://www.anu.edu.au/EMU



} } } Warren E Straszheim {wesaia-at-iastate.edu} 10/10/01 03:30AM } } }
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I would be interested to hear of any IP solution you come up with.

However, I would think that running the scope in environmental mode at
higher voltage ought to be able to give you a satisfactory image.

We have a Hitachi S2460N here. We routinely run 40 Pa of Helium to
eliminate charging. At more than 10 kV, we can get a suitable image off of
our Robinson BSE. Our Oxford Tetra(tm) allows us to drop the voltage
further. I will forward you an image of a bug from our scope under separate
cover.

Warren

At 09:29 AM 10/9/2001 -0700, you wrote:

} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image? I am referring to the streak across the
} image manifested as a change in density. As an example, have a look at
} this uncoated mite:
}
} http://katie.ucdavis.edu/pics/sucker2.jpg
}
} The nature of this projects requires that we place the live mite in the
} scope and get a picture in the first 5 minutes. This picture was taken on
} a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
} for publication. I am looking for some help in eliminating some of the
} charging artifacts without losing too much detail. Any help will be
} appreciated.
}
} We are using PS 6 and I have the IPTK plugins. I made some progress using
} a Gaussian filter to produce a very fuzzy duplicate image, invert the
} image, add it to a layer and then adjust the opacity to around 50%. This
} was only partially successful. Any help will be appreciated.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking






From daemon Wed Oct 10 10:06:38 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Wed, 10 Oct 2001 09:58:16 -0500
Subject: Re: Photoshop and SEM charging

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Rick,

I'm not an expert at this, but I do play with images in photoshop for
my own amusement.

I played with your image in Photoshop and the best I could do was reduce
the difference in darkness between the top and the bottom of the picture
by lasooing the top portion of the picture (at 200X mag.) with the lasoo
tool and then using the brightness-contrast setting under Image, Adjust,
to get the top and bottom portions of the photo to match in
brightness. This still left the lines, which I tried the gausian blurr
on, but it couldn't be blurred much before it looked out of place. (like
a blurry band in the middle of an otherwise crisp the picture.)

This is just another trick you might try. Good luck.

Karen Pawlowski

Rick Harris wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image? I am referring to the streak across the
} image manifested as a change in density. As an example, have a look at
} this uncoated mite:
}
} http://katie.ucdavis.edu/pics/sucker2.jpg
}
} The nature of this projects requires that we place the live mite in the
} scope and get a picture in the first 5 minutes. This picture was taken on
} a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
} for publication. I am looking for some help in eliminating some of the
} charging artifacts without losing too much detail. Any help will be
} appreciated.
}
} We are using PS 6 and I have the IPTK plugins. I made some progress using
} a Gaussian filter to produce a very fuzzy duplicate image, invert the
} image, add it to a layer and then adjust the opacity to around 50%. This
} was only partially successful. Any help will be appreciated.
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu


From daemon Wed Oct 10 10:17:49 2001



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 10 Oct 2001 08:07:45 -0700
Subject: High Voltage Cable repair

Contents Retrieved from Microscopy Listserver Archives
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} Dear Listers,
}
} Thank you to all who have responded to my request for high voltage cable
repair.
}
} The results were:
}
} 1. Fix it yourself.
} 2. Buy another JEOL 35C for the cable.
} 3. Have a third party repair the cable.
}
} The consensus is that the best third party repair is:
}
} } Dielectric Sciences Inc.
} } 88 Turnpike Road
} } Chelmsford, MA 01824
} } Phone: 978 250-1507, FAX: 978 250-1699
}
}
} Best Regards,
}
} Earl Weltmer



From daemon Wed Oct 10 11:14:49 2001



From: Nora Pratta- Silvia Montoro :      csedax-at-ceride.gov.ar
Date: Wed, 10 Oct 2001 12:27:25 -0300
Subject: SEM: distortion on digital images

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Dear members of the list,


We need some help for solving a problem related to the deformation of the
image taken with our SEM . We have already asked a number of people about
this but, it seems the solution is not so straightforward.


We have a JEOL SEM 35C, from the early 804s. Recently, we installed the
SemAfore digital acquisition system which gets the electronic signal
directly out of the column and sends it to the computer. The CRT4s show the
same analogic image as always.


The distorsion of the image appears at the beginning of every scanline ramp
(lefthand side on the image). Two micrographs digitally taken from latex
microspheres give the idea what the problem looks like: the same feature (a
group of microspheres) is taken positioning them at the very left-hand side
of the image, then moved to the right-hand side of the same CRT screen. It
is clear that the distortion on the shape of the spheres appears on the
first image (on the second one, you see a very very little deformation to
the opposite direction). Find both micrographs at:

http://www.ceride.gov.ar/servicios/sem/kuqui1.jpg

http://www.ceride.gov.ar/servicios/sem/kuqui2.jpg



The technicians at our institute tested with the oscilloscope the shape of
the signal out of the scan generator unit. See, at:


http://www.ceride.gov.ar/servicios/sem/ the file:
dibujo_mail.bmp


a drawing is seen: in blue the theoreticall scan generator ramp, while in
magenta the real one as shown on the oscilloscope. The scanning ramp showes
a distortion on the shape starting from the begining up to the 8% of the
leght of the ramp, along a scanline (see the magenta dip inside the green
square). From that point, the ramp followes the shape that should have up to
the end of the scanline. Rounded corners at the beginning and the end of the
ramp are commonly found, according to the technicians.


This problem was seen on the CRT screen since long ago, but since the image
on the CRT is diminished in size, the defect is not
so obvious nor so serious. The digital imaging system gets and showes on the
PC screen the complete image, made up by the complete scan along a line and,
along the frame. In these conditions, the distortion shown on the digital
images is dramatical.


The technicians say that a sort of over scan made via software, once the
image is collected, could help. But, that solution reduces the side of the
viewing field and takes extra imaging processing work.


Any suggestion or help will be very much appreciated, thanks in advance.



Silvia Montoro- Nora Pratta
Centro Regional de Investigaciones y Desarrollo de Santa Fe (CERIDE)
G|emes 3450
3000 Santa Fe
Argentina
csedax-at-ceride.gov.ar
----------------------------------------------------------------------------
----







From daemon Wed Oct 10 11:44:27 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 10 Oct 2001 12:37:46 -0400 (EDT)
Subject: EM film casettes

Contents Retrieved from Microscopy Listserver Archives
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I have unearthed 2 EM film casettes (the boxes that store film in a
TEM). They have no identifying marks. They are black, about 5 1/2 x 4
3/8 x 2 1/4 inches. The black metal top that slides out is about 5 1/2
x } 6 7/8 inches. They do not belong to Philips or JEOL microscopes, but I
cannot determine where they originated, perhaps Zeiss? or Hitachi? If
anyone thinks (s)he can use them, please reply directly, and I will
send them to you. Otherwise, they will have to take up some space in the
landfill.

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Oct 10 12:02:07 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 10 Oct 2001 11:56:10 -0500
Subject: Re: SEM: distortion on digital images

Contents Retrieved from Microscopy Listserver Archives
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Your effect looks like there is not enough fly-back delay for your SEM
under your present operating conditions. We can see the same foreshortening
and shifting on the monitor of our JEOL 840A as we switch through the
various scan speeds. The effect is particularly pronounced at TV-rate
scans. It is present, but not so bad, at the SR (super-rapid?) scan rate,
and it is not apparent at the Slow-1 or Slow-2 scan settings.

I am not familiar with the SemAfore system. Is is an active system (takes
control of the SEM scan) or a passive system (just digitizes the regular
SEM scan)? If it is an active system, there should be an adjustment to
increase the fly-back delay before the next horizontal scan starts. If they
don't provide such an adjustment, they should add one. If it is a passive
system, then you should slow down your SEM's scan.

Warren

At 12:27 PM 10/10/2001 -0300, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

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Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Wed Oct 10 12:22:57 2001



From: Julie Gross :      jgross-at-neuron.uchc.edu
Date: Wed, 10 Oct 2001 13:01:49 -0400
Subject: TEM of labelled, transplanted cells

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Dear colleagues,
I don't know if this thread has already come up, but here goes:
Does anyone have tips on using EM to identify transplanted cultured cells
labelled
with latex beads in a host animal. Especially to see if synapses are being
formed.
Would traditional methods destroy the latex? if so, are there other labels
for the
cultured cells? Thanks in advance,
Julie Gross
Dept. of Neuroscience
UCONN Health Center
Farmington, CT 06030



From daemon Wed Oct 10 12:22:58 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Wed, 10 Oct 2001 12:20:09 -0500
Subject: Re: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,

To follow up on two points mentioned by others:

1. Brad Johnson wrote: "Your best bet is to avoid/minimize saturation when
you capture your images A "wet" ESEM is fantastic for this. As you
probably know, try to minimize spot size, current, and voltage to minimize
charging artifacts..."

Your S3500N is well equiped to do this. Consistent with the above
suggestions, have you also been playing around with the mites in the
variable pressure mode? In VP mode, use your backscattered electron image
which inherently shows much less charging artefact thae SE imaging, and by
adjusting the pressure you may be able to eliminate charging. In BSE mode,
you may need higher kV, like 5-10 kV, to "see" through the gas in the VP
mode, and in order for BSE's to be detected by your BSE detector.

If you have the ESED mode on your S3500N, that will enable you to to get a
SE-like image in VP mode. Still, at the low mags you are working at, BSE
should do fine for sufficent resolution, and may be easier to get rid of
charging in that mode.

You may even be able to succeed using BSE image in high vacuum mode, if the
mite doesn't dry out too fast..

2. Ken Converse wrote: "Also, the horizontal scan speed has a much greater
effect on charging than the vertical speed. If you run a faster horizontal
rate and more lines (slower vertical speed), this will help."

Again on your S3500N, you have that Image Integration mode that often can be
used to eliminate charging in whatever scope mode you are operating in.
Select the slowest line scan rate such that you observe no charging bands on
your monitor. If you still see some bands, select next fastest rate. Then go
to Image Integration set-up and select the number of frames to add tgether,
each recorded at the scan rate you just set, usually something like 4-10
will work.

Hope this helps, and good luck. Let us all know what works for you.

Gib

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/

} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image?...snip!...

} The nature of this projects requires that we place the live mite in the
} scope and get a picture in the first 5 minutes. This picture was taken on
} a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
} for publication. I am looking for some help in eliminating some of the
} charging artifacts without losing too much detail. Any help will be
} appreciated.

}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu




From daemon Wed Oct 10 12:44:13 2001



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Wed, 10 Oct 2001 13:37:35 -0400
Subject: Re: SEM: distortion on digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} This problem was seen on the CRT screen since long ago, but since the image
} on the CRT is diminished in size, the defect is not
} so obvious nor so serious. The digital imaging system gets and showes on the
} PC screen the complete image, made up by the complete scan along a line and,
} along the frame. In these conditions, the distortion shown on the digital
} images is dramatical.

I think your clue is in the statement above. If the defect can be seen on
the SEM's CRT screen, no mater how small, then your SEM has a scan
generator problem. SemaAfore (a JEOL product) is a passive scan digitizer.
It can only do as good as the 35C's scan generator. Your 35C's scan
generator is producing ramps that are non-linear at the start and end of
the ramp. Like the tech said, thats not abnormal.

What's missing from your ramp diagram is where does the SEM starts using
the ramp signal for image display. What happens is the ramp is non-linear
at the start but the SEM has a little delay after the start to get into the
linear range before using the ramp for image display. The horizontal
blanking signal defines this region.

You need to look at the horizontal blanking signal to see the real
start/end of ramp location. If you are still non-linear in that ramp
region, then the tech needs to fix this first on the SEM.

SemAfore might have a software param to add a delay after the horizontal
blanking signal. This might fix the acquired image but you are better off
fixing the SEM first.

Scott


-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Oct 10 14:34:10 2001



From: Chen Chen :      cche1-at-mail.jhmi.edu
Date: Wed, 10 Oct 2001 15:18:49 -0400 (EDT)
Subject: looking for an old paper

Contents Retrieved from Microscopy Listserver Archives
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Dear list member,
I am looking for an old paper, Th. Koller, et al., Micron 1:110, (1969).
This journal is not available in Hopkins.
Does anyone would lile to help me?
thanks


Chen Chen

Department of Biological Chemistry
The Johns Hopkins University
School of Medicine
725 N. Wolfe Street
Baltimore, Maryland 21218



From daemon Wed Oct 10 14:51:04 2001



From: Tina Schwach :      tschwach-at-mindspring.com
Date: Wed, 10 Oct 2001 14:51:25 -0500
Subject: Re: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,
I am assuming you want to fix this image rather than go collect another one.
It depends on how much time and effort you wish to put into this project.
One way, that I have used rather successfully has been a combination of the
clone tool with a small brush size and just a little feathering of the edge,
brightess/contrast adjustment, and a little spot blurring. Do each change
as a separate layer leaving your original intact. It will take some time
but I can show you before and after images (much worse than yours since
molds tend to charge like heck) that you'd never know where charging.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112



From daemon Wed Oct 10 15:05:22 2001



From: Johnson, Bradley R :      Bradley.Johnson-at-pnl.gov
Date: Wed, 10 Oct 2001 12:59:46 -0700
Subject: RE: distortion on digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Silvia,
We recently had an EDS mapping and imaging upgrade done to our SEM.
When the technician installed the imaging hardware and software, there were
several adjustments made (using software settings) to select the field of view
that was digitally captured. As I watched the field engineer work, it was
apparent that the area selected for image capture was smaller than the scanned
area. Roughly speaking, it appeared that the scanned area was about 10-15%
larger than what was captured. Possibly you could make some adjustments to the
image capture area such that your digital image field of view would be the same
as your analog, while retaining a larger scanned area. Since every manufacturer
has their own way to accomplish these things, you would probably need to get the
specifics directly from them. Good luck.

-Brad

----------
From: Nora Pratta- Silvia Montoro
Sent: Wednesday, October 10, 2001 8:27 AM
To: microscopy-at-sparc5.microscopy.com
Cc: csedax-at-ceride.gov.ar
Subject: SEM: distortion on digital images

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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Dear members of the list,


We need some help for solving a problem related to the deformation of
the
image taken with our SEM . We have already asked a number of people
about
this but, it seems the solution is not so straightforward.


We have a JEOL SEM 35C, from the early 804s. Recently, we installed
the
SemAfore digital acquisition system which gets the electronic signal
directly out of the column and sends it to the computer. The CRT4s show
the
same analogic image as always.


The distorsion of the image appears at the beginning of every scanline
ramp
(lefthand side on the image). Two micrographs digitally taken from latex
microspheres give the idea what the problem looks like: the same feature
(a
group of microspheres) is taken positioning them at the very left-hand
side
of the image, then moved to the right-hand side of the same CRT screen.
It
is clear that the distortion on the shape of the spheres appears on the
first image (on the second one, you see a very very little deformation
to
the opposite direction). Find both micrographs at:

http://www.ceride.gov.ar/servicios/sem/kuqui1.jpg

http://www.ceride.gov.ar/servicios/sem/kuqui2.jpg



The technicians at our institute tested with the oscilloscope the shape
of
the signal out of the scan generator unit. See, at:


http://www.ceride.gov.ar/servicios/sem/ the file:
dibujo_mail.bmp


a drawing is seen: in blue the theoreticall scan generator ramp, while
in
magenta the real one as shown on the oscilloscope. The scanning ramp
showes
a distortion on the shape starting from the begining up to the 8% of the
leght of the ramp, along a scanline (see the magenta dip inside the
green
square). From that point, the ramp followes the shape that should have
up to
the end of the scanline. Rounded corners at the beginning and the end of
the
ramp are commonly found, according to the technicians.


This problem was seen on the CRT screen since long ago, but since the
image
on the CRT is diminished in size, the defect is not
so obvious nor so serious. The digital imaging system gets and showes on
the
PC screen the complete image, made up by the complete scan along a line
and,
along the frame. In these conditions, the distortion shown on the
digital
images is dramatical.


The technicians say that a sort of over scan made via software, once the
image is collected, could help. But, that solution reduces the side of
the
viewing field and takes extra imaging processing work.


Any suggestion or help will be very much appreciated, thanks in advance.



Silvia Montoro- Nora Pratta
Centro Regional de Investigaciones y Desarrollo de Santa Fe (CERIDE)
G|emes 3450
3000 Santa Fe
Argentina
csedax-at-ceride.gov.ar

----------------------------------------------------------------------------
----









From daemon Wed Oct 10 16:01:43 2001



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Wed, 10 Oct 2001 10:54:45 -0700
Subject: Edwards 505 meter

Contents Retrieved from Microscopy Listserver Archives
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Dear List,
It seems the meter, model 505, for my Edwards penning gauge has bit the
dust. Edwards no longer sells nor works on the 505's so getting it
repaired doesn't seem to be an option. Would anybody perhaps have one of
these "obsolete" meters gathering dust on their shelf? I would
appreciate hearing from you. Thanks.
Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================





From daemon Wed Oct 10 17:06:23 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Wed, 10 Oct 2001 17:59:36 -0400
Subject: LM: Two-Photon Excitation Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Dear List,

I just heard about Two-Photon (or Multi-Photon) excitation microscopy.
Does anybody know of a web site that has basic information about
this technique, descriptions of applications, and the advantages.
Something like "Two-Photon Microscopy for Dummies" would do.

This has always been such a terrific source of information.

Thank you,
Darrell



From daemon Wed Oct 10 17:12:11 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Wed, 10 Oct 2001 16:07:31 -0600 (MDT)
Subject: SKI antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone used the anti-SKI antibody from Santa Cruz? Or any other
commercial antibodies to SKI (and/or SKIP)?

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|




From daemon Wed Oct 10 19:12:36 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 11 Oct 2001 11:53:20 +1000
Subject: Embedding resins/confocal/fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The distortion is caused by the scan coils "ringing".

When an SEM (or imaging system) acquires an image, it rasters from left to
right then restarts at the left side of the screen again.

It is the "retrace", from right to left, at a relatively high speed that
causes the scan coils to generate "back emf" or "ringing".

SEM manufacturers usually "blank" ,or turn off, the video during the
retrace and part of the first few milliseconds of video. The effect is that
you lose part of the image that is distorted.

What can be done to resolve your problem?
Slower horizontal scan speed would help.
Better yet, ask the vendor to blank the image just as the SEM manufacturers
do.

Hope this helps,

Earl





----- Original Message -----
} From: "Johnson, Bradley R" {Bradley.Johnson-at-pnl.gov}
To: {microscopy-at-sparc5.microscopy.com} ; "'Nora Pratta- Silvia Montoro'"
{csedax-at-ceride.gov.ar}
Sent: Wednesday, October 10, 2001 12:59 PM


I would appreciate any information about resins that have been found best for
embedding of tissues for confocal microscopy. Of particular interest are lack
of autofluorescence and quenching.
Thank you
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com



From daemon Thu Oct 11 07:41:35 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 11 Oct 2001 08:34:05 -0400
Subject: Re: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
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Brad,
I'm not familiar with the S3500N, but most SEMs have a means of setting
your record scan speed and this usually involves up to 3 different
settings: 1.) Number of lines per frame (vertical rate), 2.) number of
points per line (part of horizontal rate) and 3.) dwell time per point
(the other part of horizontal rate) Sometimes the last two are
combined in a single adjustment of time per line.

This adjustment is usually different from the viewing scan rate adjustment.

Perhaps someone who is familiar with this SEM can tell you how to make
the adjustments

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


Johnson, Bradley R wrote:

} HI Ken,
} I read your message, and the comment about adjusting scan speed
} interests me. How would one do that?
}
} -Brad
}
} } ----------
} } From: Ken Converse
} } Sent: Tuesday, October 9, 2001 3:54 PM
} } To: Rick Harris; MSA, listserver
} } Subject: Re: Photoshop and SEM charging
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Rick,
} } My experience is that live insects don't generally charge because the
} } evaporating water carries the charge away. From the looks of the image,
} } I think you were using a lot of beam current. Cur that back a whole lot
} } and the charging will probably go away. Also, the horizontal scan
} } speed has a much greater effect on charging than the vertical speed. If
} } you run a faster horizontal rate and more lines (slower vertical speed),
} } this will help.
} }
} } Ken Converse
} } owner
} } Quality Images
} } third party SEM service
} } Delta, PA
} }
} } Rick Harris wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Can anyone offer any ideas on the best way to proceed with removing
} } } the effects of charging on an image? I am referring to the streak
} } } across the image manifested as a change in density. As an example,
} } } have a look at this uncoated mite:
} } }
} } } http://katie.ucdavis.edu/pics/sucker2.jpg
} } }
} } } The nature of this projects requires that we place the live mite in
} } } the scope and get a picture in the first 5 minutes. This picture was
} } } taken on a Hitachi S3500N at 2.5 KV. The picture suits our needs but
} } } is too poor for publication. I am looking for some help in
} } } eliminating some of the charging artifacts without losing too much
} } } detail. Any help will be appreciated.
} } }
} } } We are using PS 6 and I have the IPTK plugins. I made some progress
} } } using a Gaussian filter to produce a very fuzzy duplicate image,
} } } invert the image, add it to a layer and then adjust the opacity to
} } } around 50%. This was only partially successful. Any help will be
} } } appreciated.
} } }
} } }
} } }
} } } Rick A. Harris, Director
} } } Microscopy and Imaging Facility
} } } Section of Molecular and Cellular Biology
} } } 1241 Life Sciences Addition
} } } University of California
} } } Davis, CA
} } } 530 752 2914
} } } 530 754 7536 fax
} } } http://katie.ucdavis.edu
} } } raharris-at-ucdavis.edu
} } }
} } }
} } }
} } }



From daemon Thu Oct 11 07:41:37 2001



From: hagglund.kw-at-pg.com
Date: Thu, 11 Oct 2001 08:27:48 -0400
Subject: Re: SEM: distortion on digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Nora,

The distortion you have looks like a problem with the syncronicity of the x-y
scan control and beam blanking. As the scan moves across the specimen, the beam
physically moves. The electronics of the microscope "blank" the signal as the
beam drops down one scan line and returns to the left hand side of the
micrograph. If the blanking mechanism is slightly out of sync with the scan
coils, the signal turns on while the beam is still moving from left to right.
The distortion is alway at the left hand side of the micrograph and appears as a
mirror image on the left and right sides of a blur. It usually is worse at
faster scan rates. You may be able to get rid of the distortion by using a
slower scan speed.

Karl Hagglund
(513) 634-0146



From daemon Thu Oct 11 08:42:53 2001



From: David Spector at Cold Spring Harbor Laboratory :      spector-at-cshl.org
Date: Thu, 11 Oct 2001 09:25:52 -0400
Subject: Position Available: Biological Microscopy Core Manager

Contents Retrieved from Microscopy Listserver Archives
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Cold Spring Harbor Laboratory on the north shore of Long Island, New
York is seeking an experienced and responsible Biological Microscopy
Facility Core Manager for the laboratory's state-of-the-art central
microscopy facility. The individual should have practical expertise
in transmission electron microscopy, confocal and widefield
fluorescence microscopy, and digital imaging. The successful
candidate will be involved in designing and carrying out experimental
protocols for users, training individuals in the use of various
microscopes, and aligning microscopes and keeping the facility
operating at an efficient and high level of productivity. Interested
individuals should send their resume, including a description of
their expertise and the names and addresses of 3 references to: Dr.
David L. Spector, Cold Spring Harbor Laboratory, One Bungtown Road,
Cold Spring Harbor, New York 11724, email: spector-at-cshl.org
--
Dr. David L. Spector
Cold Spring Harbor Laboratory
One Bungtown Road
Cold Spring Harbor, New York 11724
Tel. (516) 367-8456
Fax (516) 367-8876
email: spector-at-cshl.org


From daemon Thu Oct 11 09:04:43 2001



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 11 Oct 2001 08:57:41 -0500
Subject: Re: LM: Two-Photon Excitation Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Greetings Darrell,
There are some good resources for learning more about multiphoton
microscopy on the web. Gaining a solid conceptual understanding of the
theory requires learning a little (and later, a lot) about the lasers used
in this modality of optical sectioning microscopy. Here are some of the
best resources I've come across:

http://swehsc.pharmacy.arizona.edu/exppath/micro/confocal.html
(a great starting point for links to optical sectioning microscopy)

http://microscopy.fsu.edu/primer/techniques/fluorescence/multiphoton/multiphotonhome.html
(has excellent animated renditions of the concepts)

http://www.coherentinc.com/cohrLasersAPPLICATIONS/assets/applets/CLGMPE.pdf
(a PDF document from coherent, inc., a provider of lasers for multiphoton
microscopy)

and you may find some helpful information and links on our site (I'm
presently putting together some pertinent instructional material for our
website):

http://www.itg.uiuc.edu/

If you have any specific questions, I'll be happy to try and answer them
for you.

-Karl G.


_______________________________________________
Karl Garsha
Specialist in Light Microscopy
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Thu Oct 11 09:33:11 2001



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Thu, 11 Oct 2001 10:27:53 -0400
Subject: RE: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This thread on Photoshop "enhancement" of electronic raw date brings to
light an area of constant concern within my laboratory. At what point have
we over "enhanced" the image, creating/altering features which potentially
do not exist within the sample? It seems clear that any attempt to clone,
paint, or copy/ paste fall into the category of digital artistry and should
be avoid. In fact, most of the respondents have attempted to address the
reduction/elimination of sample charging within the SEM, thus eliminating
the need for image manipulation. Due to the highly regulated environment of
the pharmaceutics industry we must adhere to strict FDA guidelines so our
interpretations tend to be on the extreme side. That being said, my
recommendation is to be very careful with the use of photoshop, it is a
great product for "manipulating" images but our data should not be subject
to artistic license. Switching directions a bit, we do employ filtering
routines when analyzing data. We attempt to insert appropriate controls
into the method to catch any unanticipated events. I would be very
interested in the opinions of others in regards to where they draw the line
on image manipulation and how they rationalize their position.

Thank you, jr

} -----Original Message-----
} From: Rick Harris [SMTP:raharris-at-ucdavis.edu]
} Sent: Tuesday, October 09, 2001 12:30 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Photoshop and SEM charging
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image? I am referring to the streak across the
} image manifested as a change in density. As an example, have a look at
} this uncoated mite:
}
} http://katie.ucdavis.edu/pics/sucker2.jpg
}
} The nature of this projects requires that we place the live mite in the
} scope and get a picture in the first 5 minutes. This picture was taken on
}
} a Hitachi S3500N at 2.5 KV. The picture suits our needs but is too poor
} for publication. I am looking for some help in eliminating some of the
} charging artifacts without losing too much detail. Any help will be
} appreciated.
}
} We are using PS 6 and I have the IPTK plugins. I made some progress using
}
} a Gaussian filter to produce a very fuzzy duplicate image, invert the
} image, add it to a layer and then adjust the opacity to around 50%. This
} was only partially successful. Any help will be appreciated.
}
}
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu
}


From daemon Thu Oct 11 09:34:35 2001



From: Judy Bowen :      jabowen-at-buckman.com
Date: Thu, 11 Oct 2001 09:34:27 -0500
Subject: Re: Nikon Objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Nikon Labophot II and was recently told that 2.5X was the lowest
mag objective that was made. However, I don't know anything about the
Microphot. You can post a "want to buy" add for free on labx.com. I had a
very good experience doing that a short time ago. You can also do an
internet search for used microscopes. You will get a lot of hits. I have
found many of the used equipment dealers to be very nice and helpful.

Judy Bowen

P.S. I think you are located at SIU Springfield. I was there from 1987-89.
I was Judy Rapp then and did a little EM work. I was a friend of Donna's.
Send me an e-mail at jabowen-at-buckman.com if any of this is familiar.

}
} Hi,
}
} Our facility is looking to purchase a dry 1x objective for a Nikon
Microphot
} light microscope. I have contacted a few vendors and have been told that
} the microscope is out of date and it will be impossible to locate such an
} objective. Any help in locating this item will be appreciated.
}
} Thank you in advance for your help
}
} aruna
}



From daemon Thu Oct 11 15:19:33 2001



From: donsheri695-at-yahoo.com ()
Date: Thu, 11 Oct 2001 15:08:11 -0500
Subject: Ask-A-Microscopist: draw tube on a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (donsheri695-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
October 11, 2001 at 15:06:46
---------------------------------------------------------------------------

Email: donsheri695-at-yahoo.com
Name: Justin Kirkendall

Organization: Shawnee Middle School

Education: 6-8th Grade Middle School

Location: Easton, Pensylvania, USA

Question: What is the draw tube on a microscope used for?

---------------------------------------------------------------------------


From daemon Thu Oct 11 15:20:01 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Thu, 11 Oct 2001 13:15:34 -0700
Subject: Re: TEM of PLGA nanoparticles and label

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I assume that a good soluble organometal is easier to incorporate than a suspension of powder etc, esp for such small particles (the ruthenium red powder may be larger than your nanoparticles?), but I could easily be wrong. Sounds like you don't want to just react the stain with the particle surface before doing the study, right?

What about tetraethyl lead? Check into the catalysts (e.g. stannous octoate) used to polymerize the plga or other polymers.

I'm interested, and I would appreciate it if you could let me know what is decided upon.

Richard

Richard Thrift
Richard_Thrift-at-SkyePharma.com
SkyePharma, Inc
10450 Science Center Drive
San Diego, CA, 92121 USA

} } } {"tbargar-at-unmc.edu"-at-sparc5.microscopy.com} 10/10/01 6:19:05 AM } } }
Hi everyone,

One of our pharmacology researchers is making nanoparticles ranging in
sizes from 50nm up to 250nm. The material is
polylactic-acid-coglycolic-acid (PLGA). He's looking for a lipophilic
electron dense particle, dye or stain that could be incorporated into the
particles during their formation and which could be seen in the TEM
sections. The material has to be able to be suspended or dissolved in an
organic solvent.

For our part here are some of the things we've thought of using: Ruthenium
Red powder, colloidal gold in 5 to 10nm range, phosphotungstic acid in
ethanol.

His material will also melt at 45 degrees centigrade. So I embedded in
Unicryl, UV polymerization at 4 degrees centigrade. Does anyone know if
there is still heat produced during the polymerization?

This seems to be in the realm of polymer chemistry and materials science.
I'd appreciate any and all help, Thanks.

Tom Bargar
UNMC EM Lab
402-559-7347





From daemon Thu Oct 11 15:44:52 2001



From: ramos-at-argo-tech.com
Date: Thu, 11 Oct 2001 16:38:27 -0400
Subject: Nikon Objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Aruna,

Try "M & R Optical" -- Sales, Service and Calibration of all Makes and
Models. The persons name is Paul Musci. He services all of our optical
microscopes (all of which are older pieces of equipment).

Here is the pertinent information:
Paul Musci
64 Osgood Rd.
P.O. Box 515
Charlton City ,MA 01508

Tel. (508)248-5684
Fax.(508)248-4658




OR PERHAPS you could try--} A place called PYE Metallurgical Consulting,
INC.
One of the things this business specializes in is used and refurbished
equipment. Perhaps (maybe) they could get this objective for you, or at
least point you in the right direction......

552 Cole Drive
Meadville, PA 16335
Tel. 1-814-337-0194
Fax 1-814-337-5939
www.pyemet.com
pyemet-at-toolcity.net



Kelly A. Ramos
Argo-Tech Corporation
Materials Laboratories
23555 Euclid Avenue
Cleveland, OH 44117




From daemon Thu Oct 11 16:18:08 2001



From: DCiaburri-at-gdds.com
Date: Thu, 11 Oct 2001 17:02:14 -0400
Subject: Re: B eta Probe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tony,

This sounds reminiscent of a beta backscatter detector we used to have.
Except I think we only used it for coating thickness measurements (I never
used it personally). You might want to check out ASTM B-567 Standard Test
Method for Measurement of Coating Thickness by the Beta Backscatter
Method.

Diane Ciaburri
General Dynamics
Pittsfield MA



} Tony Garratt-Reed {tonygr-at-mit.edu}
} 10/09/01 10:59 AM
}
}
} Hi, Listers!
}
} Many years ago, when (or even before) I was an undergraduate (that would
} place it in the early '60s), my aunt managed the analytical lab at a
} company that produced non-ferrous metals (principally, I think, bronzes
} and brasses). As one does in such situations, she invited me to tour
her labs
} one day.
}
} One of the instruments I was shown was called a "Beta Probe". A piece
of
} the metal was ground and polished, and was then placed face down on an
} aperture in the top plate, with what I now realize was an O-ring seal. A
} few buttons were pressed, and after a little while some of the
electronic
} counters of the day (neon tubes with 10 electrodes) began spinning
round.
} My memory is that the operator had some range of ratios between the
} recorded numbers within which he (it was, of course, a "he" in those
days,
} even though my aunt was the manager) could pass the sample as meeting
} specifications.
}
} I surmise that this was an electron milli-probe (if you'll forgive my
} coining of the term!) with preset spectrometers tuned to the elements of
} interest in the specification. I would very much like to find out more
} about this device. Happily, my aunt is still available to be asked, but
} she knows nothing of the operation of the instrument. The people who
ran
} it have either died, or lost touch (the company failed twenty years
ago).
}
} If anyone can give me more information about this instrument, I would be
} very interested, and most grateful.
}
} Thanks,
}
} Tony
}
}
} * * * * * * * * * * * * * * * * * * * * * * * * * *
} * Anthony J. Garratt-Reed M.A., D.Phil.
} * MIT, Room 13-1027
} * 77 Massachusetts Avenue
} * Cambridge, MA 02139-4307
} * USA
} * Phone: (617) 253-4622
} * Fax: (617) 258-6478
} *



From daemon Thu Oct 11 22:39:12 2001



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au
Date: Fri, 12 Oct 2001 13:30:07 +1000
Subject: thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to the people who sent replies to my 'fluorescent cell wall dyes'
question. I have success!

Mark Talbot



From daemon Fri Oct 12 04:29:10 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Fri, 12 Oct 2001 10:23:57 +0100
Subject: Re: TEM of PLGA nanoparticles and label

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In regard to the suggestion:

{ { What about tetraethyl lead? Check into the catalysts (e.g. stannous
octoate) used to polymerize the plga or other polymers. } }

I made a similar suggestion about 30 years ago, and was come down upon
like a ton of bricks. Tetraethyl lead is not only extremely poisonous,
it is rapidly absorbed by the skin. I understand that in companies
where it is used, they have a paraffin shower, and the person is thrown
under that clothes and all, no time to muck about.

Because of this, I looked up the work of J.Smid at Syracuse, NY, who
uses bismuth compounds for the same purpose. From this work I learned
about triphenyl bismuth, which appears to be a remarkably innocuous
compound (remember the old days, when people used "bismuth" as an
indigestion remedy?)

One of his published papers is:

Rawls HR, Granier RJ, Smid J, et al.
Thermomechanical investigation of poly(methylmethacrylate) containing an
organobismuth radiopacifying additive
J BIOMED MATER RES 31 (3): 339-343 JUL 1996

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+





From daemon Fri Oct 12 08:59:19 2001



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Fri, 12 Oct 2001 11:14:30 -0230
Subject: RE: Photoshop and SEM charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick writes ...

} Can anyone offer any ideas on the best way to proceed with removing the
} effects of charging on an image? I am referring to the streak across the
} image manifested as a change in density. As an example, have a look at
} this uncoated mite:
} ...

Know that you can mask this image with a "gray" and set transparency such
that the image will remain unchanged. I see several places in the original
image where the horizontal banding can be sampled as a row of vertical
pixels which range in brightness and is representative of the charging
effect. Now apply this range of grays to the gray mask. If the sampling of
banded grays includes 'white', then begin with a white mask and paint the
banding across the mask and apply the mask with varying transparency. Not
having tried this, it is possible the opposite effect might happen ... if
so, then invert the mask.

hth ... shAf :o)



From daemon Fri Oct 12 09:17:25 2001



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Fri, 12 Oct 2001 09:06:51 -0500
Subject: Re: Thanks for the help.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



To all of the netters,

Thanks for all the help with the blue Feulgen question I had not too long
ago. It was very informative.


Donald G. Awbrey, HT(ASCP) QIHC
Image Analysis / Electron Microscopy
donaldawbrey-at-texashealth.org
donaldawbrey-at-hotmail.com




From daemon Fri Oct 12 11:26:43 2001



From: Dmrelion-at-aol.com
Date: Fri, 12 Oct 2001 12:17:12 EDT
Subject: continuously variable mag for WILD M5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Does anyone know if there was an accessory for the WILD M5 stereo
microscope that provided a (small) range of continuously variable
magnification. The feature is needed to get an exact overlay of video camera
images from two different microscopes.

Thanks,

Don Marshall

Don Marshall
RELION Industries
PO Box 12
Bedford, MA 01730

cathodoluminescence and mass spectroscopy

781-275-4695 (phone)
781-271-0252 (FAX)
dmrelion-at-aol.com

"A weed is a flower out of place."


From daemon Fri Oct 12 12:10:42 2001



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 12 Oct 2001 12:00:20 -0500
Subject: SEM texts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'd love to pick up some used copies of SEM-related texts ,
such as "Electron Microscopy" by Bozzola and Russell and
"Scanning Electron Microscopy and X-Ray Microanalysis" by
Goldstein, Newberg, Joy), or other similar textbooks.
If anyone has spare copies, please contact me in private e-mail.

- John



From daemon Fri Oct 12 14:24:52 2001



From: Joanne Crudele :      Joanne.Crudele-at-unilever.com
Date: Fri, 12 Oct 2001 14:15:57 -0500 (Central Daylight Time)
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does any one use any other service companies for scope service of their
Philips EM400T TEM?

Other than FEI.

Joanne Crudele


-----Original Message-----
} From: McFaddin, Wade [SMTP:Wade.McFaddin-at-nextekinc.com]
Sent: Tuesday, October 09, 2001 7:59 AM
To: Microscopy-at-sparc5.microscopy.com


Nextek Inc. is seeking a qualified individual to join our analytical
laboratory team as a Materials/Failure Analysis Engineer. The ideal
candidate will have a B.S. in Materials Science, Chemistry, Engineering or
equivalent, and a minimum 3 years experience in the micro-electronic field;
or 5 or more years hands-on lab experience in the micro-electronic
materials/failure analysis
field. Knowledge in cross-sectional and destructive analysis of
micro-electronic assemblies and devices, and experience with analytical
instrumentation such as; SEM/EDS, Micro-FTIR, Thermal Analysis, Acoustic
and Optical Microscopy is a plus.

To learn more about Nextek Inc. visit our web site at
http://www.nextekinc.com/. For more
information about this position, call or email the following:

Wade McFaddin (256) 772-1995 ext.1064
wade.mcfaddin-at-nextekinc.com
or
Jim Chiang (256) 772-1995 ext. 1029
james.chiang-at-nextekinc.com






From daemon Fri Oct 12 15:05:18 2001



From: Wang, Dashan :      Dashan.Wang-at-nrc.ca
Date: Fri, 12 Oct 2001 15:58:32 -0400
Subject: comment on CCD camera needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Members,

We are going to purchase a CCD camera for our CM20 STEM. I need comment on
the AltraScan 1000 made by Gatan and KeenView DUAL DOCU IMAGING SYSTEM made
by SIS. Any opinion is welcome.

Regards,


Dashan Wang


From daemon Fri Oct 12 15:47:59 2001



From: Michael L. Boucher :      mboucher-at-tc.umn.edu
Date: Fri, 12 Oct 2001 15:48:21 -0500
Subject: Tech job at U of MN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a technician job, mostly related to repair and maintenance of our
scopes and other equipment. Salary is $20k to $30k per year. It will be
posted next week.

Applications should be done directly to the Univ. See their job website for
the posting:
http://www1.umn.edu/ohr/employ.html

The basic info is as follows:

Assist in an analytical laboratory consisting of electron microscopes, Ion
Beam Accelerator/spectrometer, X-ray diffraction equipment, scanning probe
microscopes, optical microscopes and other instrumentation.

0 50% Daily maintenance of above mentioned instruments and associated
sample preparation equipment

0 35% Troubleshooting and repair of instruments, vacuum equipment,
electronics and computers

0 15% Keeping labs clean, organized and adequately supplied

********************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 55 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://resolution.umn.edu
********************************************************************








From daemon Fri Oct 12 17:38:40 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 12 Oct 2001 15:30:48 -0700 (PDT)
Subject: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I've been trying to carbon coat some samples for SEM in a denton vacuum
evaporator. I used C-rods, one thinned from the original 4mm diameter to
about 1mm for a short distance (1cm) touching a flat C-rod. I manage a
few times to get 12-15 nm, but often the rod breaks, or shorts out before
I can get even 5 nm deposited. I like using the high vacuum on the
evaporator as you get a much better coating than from our sputter coater.

Can anyone send me some pointers as to how I can get a more controlled
coating with one run of the evaporator? I have to almost go to maximum
power to start getting deposition registered on the monitor. I tried
changing the spring so it has a more even gentler push on the sharpened
rod.

The rods I use after sharpening look like this:

--------| |---------------
| |
---------|
---------|
| |
--------| |--------------

Hopefully the ascii art comes out in email.


\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Oct 12 23:13:51 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 12 Oct 2001 20:49:37 -0700
Subject: Re: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon, hello

It's kind of strange that you have a problem. Your setup looks like OK. I
would recommend the following:

-try 5 mm instead 10 mm for 1 mm dia part.
-play with spring pressure and check that spring has enough "power" when
the rods will short at the end of evaporation (very usual problem).
-increase current slowly with a few stops. I would suggest 10A/10-20 sec
is OK.
-you may try graphite or carbon. Carbon is stronger mechanically and
evaporated slower and with higher current than graphite.
-both rods should be CO-AXIAL.
-1 mm "tip" should be sharpened to the conical shape approx 90 deg.

Personally, I prefer 6 mm carbon rods. One of them - with 1 x5 mm tip on
the end:

------------------I /--------------
I {== 6 mm dia
------------------I \--------------
-1x5mm

I wish you luck. Sergey


At 03:30 PM 10/12/01 -0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Sat Oct 13 14:22:03 2001



From: Damian Neuberger :      neuberger1234-at-home.com
Date: Sat, 13 Oct 2001 14:11:10 -0500
Subject: Dye Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

The Problem:

A plastic part is steam sterilized and can have a residual water drop in a
small depression in the part. All the water has to be removed before the
mfg process can continue. The proposed solution is to apply pressurized air
(or some other gas) to remove the water drop. However, when a jet of
pressurized air is applied it will blow out most of the water but leave
behind small 20-50 micrometer microdroplets. These are still a problem.

I thought that I could use a dye in the test droplet of water applied to the
part before it is subjected to the proposed air blast in an experimental
test setup. The idea would be that if any microdroplets remained I would be
able to see them under a simple macroscope. However, for such things as
food dye, when it dries, the color remains and I can't tell if it is wet or
dry.

One of the acceptable situation may be that most of the droplet is blown off
and the remaining microdroplets dry within seconds using heat lamp, dry air,
low RH in the test area, etc. So what I'm looking for is a dye that is one
color when wet and either another color when dry or no color at all (clear,
or white powder). My first attempts have been with cobalt chloride but
driving off all the mositure is difficult. Another possibility is using a
fluorescent dye that fluoresces one color when wet and has a very much
reduced fluorescence when dry or better yet a different color.

I certainly appreciate you help and all suggestions will be considered, even
if you have a totally different idea on how to determine when all the water
is gone.

Damian Neuberger



From daemon Sat Oct 13 19:21:39 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 14 Oct 2001 10:14:11 +1000
Subject: RE: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A couple of additional points:
Some evaporator give a choice of voltages and this is a important feature. For
metal evaporation 10V gives better control then a higher voltage, but this is
not suitable for carbon rods. Graphite and even more so carbon rods, are poorer
conductors than metal wire or foils, consequently evaporation is much more
likely to fail at the lower voltages.
Check your evaporator and if you are using something like 10 volts, then see if
you can tap the transformer at a higher voltage setting, I recommend 25 or 30V.
Obviously the amps required for evaporation will change.
If for daily operations changing the voltage is troublesome, then I would
rather put up with a touchy control for metal evaporation then with carbon
evaporation failures, i.e. rather use 25V for all evaporations then 10V.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, October 13, 2001 1:50 PM, Sergey Ryazantsev
[SMTP:sryazant-at-ucla.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Gordon, hello
}
} It's kind of strange that you have a problem. Your setup looks like OK. I
} would recommend the following:
}
} -try 5 mm instead 10 mm for 1 mm dia part.
} -play with spring pressure and check that spring has enough "power" when
} the rods will short at the end of evaporation (very usual problem).
} -increase current slowly with a few stops. I would suggest 10A/10-20 sec
} is OK.
} -you may try graphite or carbon. Carbon is stronger mechanically and
} evaporated slower and with higher current than graphite.
} -both rods should be CO-AXIAL.
} -1 mm "tip" should be sharpened to the conical shape approx 90 deg.
}
} Personally, I prefer 6 mm carbon rods. One of them - with 1 x5 mm tip on
} the end:
}
} ------------------I /--------------
} I {== 6 mm dia
} ------------------I \--------------
} -1x5mm
}
} I wish you luck. Sergey
}
}
} At 03:30 PM 10/12/01 -0700, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello,
} } I've been trying to carbon coat some samples for SEM in a denton vacuum
} } evaporator. I used C-rods, one thinned from the original 4mm diameter to
} } about 1mm for a short distance (1cm) touching a flat C-rod. I manage a
} } few times to get 12-15 nm, but often the rod breaks, or shorts out before
} } I can get even 5 nm deposited. I like using the high vacuum on the
} } evaporator as you get a much better coating than from our sputter coater.
} }
} } Can anyone send me some pointers as to how I can get a more controlled
} } coating with one run of the evaporator? I have to almost go to maximum
} } power to start getting deposition registered on the monitor. I tried
} } changing the spring so it has a more even gentler push on the sharpened
} } rod.
} }
} } The rods I use after sharpening look like this:
} }
} } --------| |---------------
} } | |
} } ---------|
} } ---------|
} } | |
} } --------| |--------------
} }
} } Hopefully the ascii art comes out in email.
} }
} }
} } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
} } \
} } Gordon Ante Vrdoljak Electron Microscope
} } Lab
} } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} } gvrdolja-at-nature.berkeley.edu UC Berkeley
} } phone (510) 642-2085 Berkeley CA 94720-3330
} } fax (510) 643-6207 cell (510) 290-6793
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}



From daemon Sun Oct 14 15:50:19 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 15 Oct 2001 09:31:02 GMT+1200
Subject: Re: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Gordon

I use the same method, and I have the same problem if I thin the rods
down too much. Try turning it down to taper down to 2mm over 6mm of
length, and make sure that the flat end of the other electrode
doesn't have any debris left on it from previous use. For the
flat-ended rod, I use 6.5mm diameter (I don't know whether it is
graphite or carbon, I don't think it matters for this one).

In contrast to Sergey's experience, I have found that graphite needs
to get much hotter to evaporate than does carbon, and the holder
assembly all became red hot when I tried graphite.

Jim Darley at Proscitech sells both types.

My only relationship with him is as a satisfied customer.

good luck

rtch


}
} Hello,
} I've been trying to carbon coat some samples for SEM in a denton
} vacuum evaporator. I used C-rods, one thinned from the original 4mm
} diameter to about 1mm for a short distance (1cm) touching a flat
} C-rod. I manage a few times to get 12-15 nm, but often the rod
} breaks, or shorts out before I can get even 5 nm deposited. I like
} using the high vacuum on the evaporator as you get a much better
} coating than from our sputter coater.
}
} Can anyone send me some pointers as to how I can get a more
} controlled coating with one run of the evaporator? I have to almost
} go to maximum power to start getting deposition registered on the
} monitor. I tried changing the spring so it has a more even gentler
} push on the sharpened rod.
}

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Oct 14 19:18:36 2001



From: Fauzi Mohd Som :      drfauzims-at-yahoo.com
Date: Mon, 15 Oct 2001 08:10:35 +0800
Subject: SemAfore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We would like to upgrade our PC containing SemAfore 3.02 hooked with JEOL
JSM5300. The old card is using the ISA however all the new boards do not
have the ISA but PCI only. So we've asked JEOL people to get ISA extender.
After getting the new 1 Ghz PC, hooking the ISA extender, hooking the card,
got everything hooked up ... I think the engineer told me ...
"very-very-very low signal". So we looked around and found one motherboard
with one ISA and the rest PCI. Hooked everything up. Still the same thing
happened. The engineer told us that we need to purchase the USB type which
costs a hefty sum in order to use with the new PC. So now we are back to the
old PC. My worry is what happen when the old PC konk.

Any suggestion or somebody experienced this? btw, the engineer also tried
with SemAfore 4.0 and it did not work.
}
} Regards,
}
} Fauzi
} Malaysian Rubber Board
}



_________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.com address at http://mail.yahoo.com



From daemon Mon Oct 15 07:12:22 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 15 Oct 2001 08:03:44 -0400
Subject: Re: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,
The most obvious thing, that no one has mentioned yet, is that the
non-sharpened rod should be shaped at a 45 degree angle with the face
towards your sample. The one mm rod should hit roughly in the center
of the 45 degree face. This allows a better distribution of carbon
towards your sample. 1 cm of 1mm rod seems awfully long to me. The
trade-off is distance from sample vs heating of sample. Closer gives
you more rapid coating due to the larger solid angle. Too close gives
very intense heating. Too far away can also impart too much heat
because it can take so long to get the thickness you're looking for. My
understanding is that 9-10 cm should be a good distance. Someone with
more math skill than I can probably tell you how many mm of 1mm diameter
carbon you need at that distance to get 5, 10 or 15 nm coating.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Gordon Vrololjak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I've been trying to carbon coat some samples for SEM in a denton vacuum
} evaporator. I used C-rods, one thinned from the original 4mm diameter to
} about 1mm for a short distance (1cm) touching a flat C-rod. I manage a
} few times to get 12-15 nm, but often the rod breaks, or shorts out before
} I can get even 5 nm deposited. I like using the high vacuum on the
} evaporator as you get a much better coating than from our sputter coater.
}
} Can anyone send me some pointers as to how I can get a more controlled
} coating with one run of the evaporator? I have to almost go to maximum
} power to start getting deposition registered on the monitor. I tried
} changing the spring so it has a more even gentler push on the sharpened
} rod.
}
} The rods I use after sharpening look like this:
}
} --------| |---------------
} | |
} ---------|
} ---------|
} | |
} --------| |--------------
}
} Hopefully the ascii art comes out in email.
}
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}
}
}



From daemon Mon Oct 15 07:37:22 2001



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Mon, 15 Oct 2001 07:34:50 -0500 (CDT)
Subject: POSTDOCTORAL POSITIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Two postdoctoral positions (see below) are available immediately. If
interested, send a CV including the names of referees to
ldm3-at-apollo.numis.nwu.edu

1. Quantitative Electron Microscopy

A postdoctoral position is available in the area of quantitative TEM/TED
of materials starting immediately. The research project focuses on
developing quantitative methods of solving structures from diffraction
patterns and HREM images. Analysis of diffraction patterns will be via
Direct Methods to recover estimates of the phases; analysis of HREM images
will be to develop methods to routinely perform translational/rotational
averaging. The work will involve collaboration with scientists at LBL and
elsewhere. A good background in computer programming in fortran or C is
required, as well as an understanding of dynamical diffraction theory.
Prior experience in HREM or Direct Methods would be an advantage.

2. Surface Microscopy

The position would involve work using the unique HREM/Surface Science
facility at Northwestern University (see http://www.numis.nwu.edu). The
primary research area will be metalic nanostructures on semiconductor
surfaces, although elements of the work will also overlap with other
ongoing projects involving magnetron deposition of quasicrystalline thin
films, hard coatings and oxide surface structures. A strong background in
basic TEM techniques as well as some familiarity with thin film growth,
surface science and other characterization techniques such as XPS are
important.


-------------------------------------------------------
Laurence Marks
Department of Materials Science and Engineering &
Center for Transportation Nanotechnology
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu http://www.ctn.northwestern.edu
-------------------------------------------------------
The Other Nanotubes http://focus.aps.org/open/st12.html
Boron Nitride Nanotubes http://pubs.acs.org/cen/topstory/7912/7912notw1.html




From daemon Mon Oct 15 08:06:29 2001



From: Markus F. Meyenhofer :      micro-at-superlink.net
Date: Mon, 15 Oct 2001 07:58:48 -0500
Subject: Re: C-coating samples for SEM in vacuum evap?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Gordon

I have used approx. 20 different Denton 502's for evaporation (and FE) over
the last 30 years. I also recondition and sell them. Since you are maybe
using one of them "carbon fiber Flash Evaporators"? sputter coaters, the
speed to evaporate in HV has to be SLOW.
Have sufficient spring pressure, s l o w l y bring up the current, watch
the rod (with welder's glasses or cover the bright zone by viewing over the
holder bar, DONT LOOK INTO THE BRIGHT SPOT!! Of course you know that!) until
some fine sparks appear, immediately reduce the current below sparking and
watch your indicator for appropriate coating. It works 99.9% of the times.
Regards,
Markus F. Meyenhofer
Microscopy Labs


From daemon Mon Oct 15 08:59:33 2001



From: Peter Heimann :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Mon, 15 Oct 2001 15:52:11 +0200
Subject: ? recommendation antibody against Collagen for immuno-EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,
can somebody recommend (by own positive experience!) an
antibody against collagen, e.g. Type IV-Collagen ? Antibody should
work in Immuno-EM, preferably tolerating glutaraldehyde fixed
material.

Thanks for direct short, informal e-mail to me.

Peter


**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************


From daemon Mon Oct 15 11:11:42 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 15 Oct 2001 09:01:50 -0700
Subject: Re: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gordon,
I have a very old JEOL evaporator and what I found was:
1. I sharpen my 5 mm dia. rods to 1 mm for no more than 4 to 5 mm. Flatten
the rod it rests on with the edge of a glass slide.
2. Use pressed carbon, not graphite. It heats more readily by resistance.
3. Carefully align and tighten the rods before you let the spring-loaded one
rest against the center of the fixed one. This avoids putting a twist in
your thinned, weak rod.
4. Don't knock the rods as you are putting the lid on.
I find you have to turn up the current slowly, watching through welding
goggles, just until the tip starts to spit sparks. Leave it there and it
will climb up by itself after that.
At 03:30 PM 10/12/01 -0700, you wrote:
} Hello,
} I've been trying to carbon coat some samples for SEM in a denton vacuum
} evaporator. I used C-rods, one thinned from the original 4mm diameter to
} about 1mm for a short distance (1cm) touching a flat C-rod. I manage a
} few times to get 12-15 nm, but often the rod breaks, or shorts out before
} I can get even 5 nm deposited. I like using the high vacuum on the
} evaporator as you get a much better coating than from our sputter coater.
}
} Can anyone send me some pointers as to how I can get a more controlled
} coating with one run of the evaporator? I have to almost go to maximum
} power to start getting deposition registered on the monitor. I tried
} changing the spring so it has a more even gentler push on the sharpened
} rod.
}
} The rods I use after sharpening look like this:
}
} --------| |---------------
} | |
} ---------|
} ---------|
} | |
} --------| |--------------
}
} Hopefully the ascii art comes out in email.
}
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
I hope this helps.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Oct 15 13:16:37 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 15 Oct 2001 14:07:49 -0400
Subject: Smoothing spectra -How is it done?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The DTSA manual describes the method of "smoothing" a spectrum as a 5, 7, or 9 point Savitsky-Golay polynomial. Does anyone know how this is applied. Is it similar to passing a filter through the data with weighted coefficients? I would appreciate if anyone can give me the algorithm. Thanks in advance.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Mon Oct 15 13:46:27 2001



From: DrJohnRuss-at-aol.com
Date: Mon, 15 Oct 2001 14:39:46 EDT
Subject: Re: Smoothing spectra -How is it done?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 10/15/01 2:27:04 PM, walck-at-ppg.com writes:

} The DTSA manual describes the method of "smoothing" a spectrum as a 5,
} 7, or 9 point Savitsky-Golay polynomial. Does anyone know how this is
} applied. Is it similar to passing a filter through the data with weighted
} coefficients? I would appreciate if anyone can give me the algorithm.
} Thanks in advance

That is exactly what it is. The S&G coefficients allow fitting polynomials of
various degrees (e.g. a quadratic polynomial) to data in order to smooth it.
The 7 point coefficients for a quadratic, for example, are
-.0952
.1429
.2857
.3333
.2857
.1429
-.0952
to be multiplied by each group of the histogram values in order to produce
one new point at the center of the 7. This is then repeated at every point to
generate a new, smoother curve.



From daemon Mon Oct 15 14:49:41 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Mon, 15 Oct 2001 15:41:31 -0400
Subject: Food Structure and Functionality Discussion site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, all

I would like to announce the debut of the Food Structure and Functionality Discussion group at the AOCS (Americal Oil Chemists Society) website, webaddress:

http://www.aocs.org/ubbcgi/ultimatebb.cgi

This discussion group is open to all agri-food researchers from Government, Industry and universities to discuss topics of interest, problems, sample preparation, etc. much like the function of this Microscopy listserver, as well as being a source of information for the activities of the Food Structure and Functionality Forum - a division of AOCS, and its yearly Symposium.

We want to make it an interesting and valuable resource for anyone who visits it. Please visit the site today and start or join a discussion! The site is up and ready for you to use.

Kind regards,

Paula Allan-Wojtas, Chair
Food Structure and Functionality Forum - a division of AOCS

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Mon Oct 15 14:53:57 2001



From: DrJohnRuss-at-aol.com
Date: Mon, 15 Oct 2001 16:05:19 EDT
Subject: Re: Smoothing spectra -How is it done?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks, John.

I was going to take a look at your book and see what they were across the center row for an image Gaussian smoothing filter if nobody replied. Somebody is currently borrowing my copy however. (Fred, if you are reading this, it is time to return it.)

Let me bother you with another question and I made it public on the Listserver. How do you handle the ends of the data array, i.e. in a 1024 array, the indices 1-3 and 1022-1024. When you apply the coefficients, they sum to one, so you can't just use some of them.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: DrJohnRuss-at-aol.com [mailto:DrJohnRuss-at-aol.com]
Sent: Monday, October 15, 2001 2:40 PM
To: walck-at-ppg.com; microscopy-at-sparc5.microscopy.com



In a message dated 10/15/01 3:49:53 PM, walck-at-ppg.com writes:

} I was going to take a look at your book and see what they were across the
center row for an image Gaussian } smoothing filter if nobody replied.
Somebody is currently borrowing my copy however. (Fred, if you are } reading
this, it is time to return it.)

Yes, they are in the book. Tell him to buy his own copy!

} Let me bother you with another question and I made it public on the
Listserver.
} How do you handle the ends of the data array, i.e. in a 1024 array, the
} indices 1-3 and 1022-1024. When you apply the coefficients, they sum to
} one, so you can't just use some of them.

Edges are always a problem. The simplest solution is to consider them a
mirror, so that the -1, -2, -3 positions have the same values as +1, +2, +3
(and the same thing at the other end). The other common choices are to
perform some kind of extrapolation on the data at each end (the simplest of
which is linear) or to make your addressing wrap around (i.e., -1 = 1023, -2 =
1022, etc.) which is not appropriate for most cases.

In any case, you can expect to lose some data at the ends (or for images, the
edges).



From daemon Mon Oct 15 18:08:53 2001



From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Mon, 15 Oct 2001 17:54:42 -0500
Subject: Dye Sub Printer(s)

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists
I have a two dye sublimation printers that are gathering dust in my
lab. If anyone is interested, you can make me an offer.
The the first printer circa 1994 Seiko ColorPoint professional 300 dpi
dye sublimation unit with an interface box to allow screen captures from
a computer monitor. The unit includes a spare ribbon and 50 sheets of
paper.
The second is a Fargo primera pro elite 300 dpi resolution. This unit
is a win printer, and will only work with Windows 95 or 98 (which I no
longer use).
The reason I'm not using them is that they simply cost too much to run.
I replaced both of them with a networked inkjet printer that is much
cheaper to run and suits my requirements just as well.

Both include manuals and are for sale as is. If anyone is interested
they can drop me a note.
--
Glenn

===============================================================================
Glenn Poirier 3450 University St, rm. 238
MicroAnalytical Laboratory Montreal, Qc
Earth and Planetary Sciences tel (514) 398 6774
McGill University fax (514) 398 4680
email: glennp-at-eps.mcgill.ca http://castaing.eps.mcgill.ca

++ Millenium hand and shrimp ++
===============================================================================


From daemon Mon Oct 15 18:28:17 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 16 Oct 2001 09:26:00 +1000
Subject: dust repellent

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

A physicist colleague asked if I knew how to make microscope slides
repellent so that dust and the paper fibres he is studying would not adhere
to them. Apart from applying a thin coat of some non-polar material, I
didn't have any useful suggestions - I'm usually trying to make things
stick, not vice versa.

Anyone have a more useful and/or more specific suggestion or protocol?

Thanks,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Oct 16 00:55:38 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 15 Oct 2001 22:47:05 -0700
Subject: Re: dust repellent

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Rosemary

It depends what you want: if you need to prevent ionic interactions in the
solution (like ion-pairs) make glass hydrophobic by siliconization. If you
want to remove static electricity (which is usually attracts the dust) you
may have to make glass electro-conductive (make it wet in simply case,
poly-lysine may work as well). Both recommendations are quite
controversial because siliconization make glass hydrophobic and it increase
the chance of electization when dry. I would suggest that there is no
solution for your case: dust is heterogeneous and some part of this nasty
thing will find the way to contaminate your sample. In this battle the
dust is always a winner.

Paper fibers, cellulose, has a lot of -OHs, so the nature of adhesion is
polar/ion (in the water solution). Siliconization would help.


Sergey

At 09:26 AM 10/16/01 +1000, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Oct 16 01:02:14 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 16 Oct 2001 01:53:49 +0800
Subject: Re: dust repellent

Contents Retrieved from Microscopy Listserver Archives
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on 10/16/01 7:26 AM, Rosemary White at Rosemary.White-at-pi.csiro.au wrote:

}
} A physicist colleague asked if I knew how to make microscope slides
} repellent so that dust and the paper fibres he is studying would not adhere
} to them. Apart from applying a thin coat of some non-polar material, I
} didn't have any useful suggestions - I'm usually trying to make things
} stick, not vice versa.
}
} Anyone have a more useful and/or more specific suggestion or protocol?
}
Dear Rosemary,
I don't know whether this will work for the particular application, but
many scientific supply houses make a silicone solution designed to make
glass hydrophobic. I think Sigma's is called Siliclad. Good luck to a
fellow physicist.
Yours,
Bill Tivol



From daemon Tue Oct 16 01:59:34 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Tue, 16 Oct 2001 09:00:00 +0200
Subject: Re: dust repellent

Contents Retrieved from Microscopy Listserver Archives
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Hi Rosemary,

Same situation here - we usually want things to stick - but poly-lysine is
out. What charge do the particles have - maybe there is something with the
same charge that would repel?

My other thought is PTFE/Teflon in it various presentations ("thin coat of
some non-polar material"). I use it on the moving parts of my bicycle as a
solution in spray form and also applied as a liquid solution. I can't
remember what the solvent/solvent mixture is but I would guess that it is
alcohol based. I have never tried it on microscope slides but is might work.

Would it do the job?


Med vänliga hälsningar/With best regards

Gareth

Då du älskar, älskar med glödande hetta.
Då du hatar, hata i flammande blixtar.
Då du festar, festa som om vardagen inte fanns.
Då du går, gå fort, men lämna alltid dörren på glänt......

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Tue Oct 16 03:58:51 2001



From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Tue, 16 Oct 2001 10:51:04 +0200
Subject: thanks

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Hi
I would like to thank everybody very much for your advice on the subject of {
fluorescence microscopy and TEM on same sample} . I am working on the procedure
and may come back with further questions.

So long
Andreas



From daemon Tue Oct 16 09:53:21 2001



From: Beauregard, Paul A. :      pabeauregard-at-ppg.com
Date: Tue, 16 Oct 2001 10:43:44 -0400
Subject: dust repellent

Contents Retrieved from Microscopy Listserver Archives
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RE: Silanization of glass surfaces.

Glass microscope slides can be made hydrophobic using hexamethyldisilAzane (HMDS) (Aldrich Chemical 37,921-2). Simply place the separated slides in a container like a wide mouth glass jar with an aluminum or Teflon lined cap. Add a few drops of HMDS and it will vaporize overnight in the sealed jar and react with the silanols on the surface. I prefer to heat the jar in an oven at 70° overnight to drive off residual ammonia. Leave the jar slightly cracked open.
The next day, in a hood, open and remove the slides. They will now bead water. This will be a molecular level coating and invisible.

Things like Rainex® and Aquapels® will work but can't be applied in the vapor state. You do not want to create artifacts (streaks) on the slides, so use the vapor state application of hexamethyldiSILAZANE (HMDS). HexamethylsilOXane is NOT the same material. HMDS is simple, easy to apply, and safely handled.

Use gloves, a hood, and respirator when handling these materials. Let the dropper and the opened jar with HMDS evaporate overnight in the hood and then place them in the trash the next day. Do not breath HMDS vapors at all! Read the MSDS information on handling.

I had a 'never opened bottle and wax sealed bottle' of chlorosilane spontaneously explode in a closed cabinet. It formed lots of white smoke and HCl. Nobody was around at the time it happened. This will make you a believer in using HMDS when possible.

These are my opinions only but based on my experiences.

Paul Beauregard
Senior Research Associate
Monroeville, PA 15601

-----Original Message-----
} From: Rosemary White [mailto:Rosemary.White-at-pi.csiro.au]
Sent: Monday, October 15, 2001 7:26 PM
To: Microscopy-at-sparc5.microscopy.com


Dear all,

A physicist colleague asked if I knew how to make microscope slides
repellent so that dust and the paper fibres he is studying would not adhere
to them. Apart from applying a thin coat of some non-polar material, I
didn't have any useful suggestions - I'm usually trying to make things
stick, not vice versa.

Anyone have a more useful and/or more specific suggestion or protocol?

Thanks,
Rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Oct 16 10:22:20 2001



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 16 Oct 2001 08:16:59 -0700 (PDT)
Subject: Re: C-coating samples for SEM in vacuum evap ?

Contents Retrieved from Microscopy Listserver Archives
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Wow! is this ancient history! My first paper ever was about the
determination of the thickness of carbon films correlated with optical
density. Which meant that I did a _lot_ of films, with different length
carbon rods (using an old Kinney evaporator actually). The primary result
was that OD and thickness have a linear relationship from about 5nm to
100nm. (And right now I am having a senior moment and not remembering the
exact correlation--and where is that paper now that I need it???) On the
practical side, 1mm carbon rod would give a 10nm film, and as I remember the
correlation was fairly linear between 0.5mm (5nm) up to 5mm (50nm). I
simply use the old "folded filter paper" method for evaluation, knowing that
an OD of about 1 gives a very heavy film--probably about 100nm. As Ken
says, geometry has a lot to do with the final thickness deposited, but once
you work it out for your system it should be fairly consistent. If my brain
functions and I can get some of these tired neurons to fire (for more
precise information), I'll forward it along.
Roger

On Mon, 15 Oct 2001 08:03:44 -0400, Ken Converse wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Gordon,
| The most obvious thing, that no one has mentioned yet, is that the
| non-sharpened rod should be shaped at a 45 degree angle with the face
| towards your sample. The one mm rod should hit roughly in the center
| of the 45 degree face. This allows a better distribution of carbon
| towards your sample. 1 cm of 1mm rod seems awfully long to me. The
| trade-off is distance from sample vs heating of sample. Closer gives
| you more rapid coating due to the larger solid angle. Too close gives
| very intense heating. Too far away can also impart too much heat
| because it can take so long to get the thickness you're looking for. My
| understanding is that 9-10 cm should be a good distance. Someone with
| more math skill than I can probably tell you how many mm of 1mm diameter
| carbon you need at that distance to get 5, 10 or 15 nm coating.
|
| Ken Converse
| owner
| Quality Images
| third party SEM service
| Delta, PA
|
| Gordon Vrololjak wrote:
|
| }
------------------------------------------------------------------------
| } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

| } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
| } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| }
-----------------------------------------------------------------------.
| }
| }
| } Hello,
| } I've been trying to carbon coat some samples for SEM in a denton vacuum
| } evaporator. I used C-rods, one thinned from the original 4mm diameter
to
| } about 1mm for a short distance (1cm) touching a flat C-rod. I manage a
| } few times to get 12-15 nm, but often the rod breaks, or shorts out
before
| } I can get even 5 nm deposited. I like using the high vacuum on the
| } evaporator as you get a much better coating than from our sputter
coater.
| }
| } Can anyone send me some pointers as to how I can get a more controlled
| } coating with one run of the evaporator? I have to almost go to maximum
| } power to start getting deposition registered on the monitor. I tried
| } changing the spring so it has a more even gentler push on the sharpened
| } rod.
| }
| } The rods I use after sharpening look like this:
| }
| } --------| |---------------
| } | |
| } ---------|
| } ---------|
| } | |
| } --------| |--------------
| }
| } Hopefully the ascii art comes out in email.
| }
| }
| }
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
| } Gordon Ante Vrdoljak Electron
Microscope Lab
| } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
| } gvrdolja-at-nature.berkeley.edu UC Berkeley
| } phone (510) 642-2085 Berkeley CA
94720-3330
| } fax (510) 643-6207 cell (510) 290-6793
| }
| }
| }
| }
|
|



Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
900 Rigdebury Road
Ridgefield, CT 06877
203-798-5448





_______________________________________________________
http://inbox.excite.com




From daemon Tue Oct 16 10:49:08 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Tue, 16 Oct 2001 11:43:04 -0400 (EDT)
Subject: optical microscope question

Contents Retrieved from Microscopy Listserver Archives
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Hello listers,

A local research physician's widow has contacted me to find out whether her
husband's microscope has any value or is still useful. It's a Nikon model
L-Ke (possibly with a camera attachment). Can anyone help with information?

Many thanks,
Dee





***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Tue Oct 16 11:18:51 2001



From: Karen Dye :      karen.dye-at-medtronic.com
Date: Tue, 16 Oct 2001 11:11:56 -0500
Subject: Nov. 9 Demo

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Just wanted to confirm our schedule and request driving instructions/hotel info for the Hitachi site. Also wondering which airport is best to fly into (?).

Thanks - KDD



From daemon Tue Oct 16 11:22:33 2001



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 16 Oct 2001 17:34:02 +0100
Subject: TEM Peptidoglycan staining

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Dear collegues


I need to produce clear cut high resolution pictures of the peptidoglycan
layer of E. coli. I am looking for a staining method that shows some
specificity for peptidoglycan (may be some method for polysaccharides, I
guess) while allowing high resolution immages.

Preferably the method should work with Epon-Araldite embedded material fixed
in glutaraldehyde and osmium.

Does anyone have suggestions for such a reliable stainning method?.


Dr. A.P. Alves de Matos
Curry Cabral Hospital
Lisbon
apmatos-at-ip.pt




From daemon Tue Oct 16 14:14:41 2001



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Tue, 16 Oct 2001 15:05:26 -0400
Subject: multiple specimen holder for Hitachi

Contents Retrieved from Microscopy Listserver Archives
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We were wondering if anyone else has run into this problem: we bought a
Hitachi VP S-3000N SEM. They offer a 125 mm multiple specimen holder -
essentially an aluminum disc on a small column that has 33 holes in it to
hold 33 stubs. We bought it and have now discovered that none of the
commercial vendors sell pin-type stubs that will actually fit into these
holes! The majority of stubs have pins that are 3mm in diameter. The
holes are only 2 mm in diameter. EMS can make the stubs at 13 cents a
piece but the minimum order is 100,000. Has anyone out there found a
source of stubs at a reasonable price (Hitachi offered to sell them at
approximately $25.00 a piece!) or are people just drilling the holes in the
holder to make them bigger? I'd really like to hear how other people are
handling this!

Lesley



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Tue Oct 16 14:57:41 2001



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 16 Oct 2001 12:50:38 -0700
Subject: Re: multiple specimen holder for Hitachi

Contents Retrieved from Microscopy Listserver Archives
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I made my own multi stub holder. But if I had what you have I'd head down
to the shop and drill/ream out those holes or have a competent shop guy do
it. Maybe 20-30 minutes of work, tops.


} We were wondering if anyone else has run into this problem: we bought a
} Hitachi VP S-3000N SEM. They offer a 125 mm multiple specimen holder -
} essentially an aluminum disc on a small column that has 33 holes in it to
} hold 33 stubs. We bought it and have now discovered that none of the
} commercial vendors sell pin-type stubs that will actually fit into these
} holes! The majority of stubs have pins that are 3mm in diameter. The
} holes are only 2 mm in diameter. EMS can make the stubs at 13 cents a
} piece but the minimum order is 100,000. Has anyone out there found a
} source of stubs at a reasonable price (Hitachi offered to sell them at
} approximately $25.00 a piece!) or are people just drilling the holes in
} the holder to make them bigger? I'd really like to hear how other people
} are handling this!
}
}


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue Oct 16 16:06:08 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 16 Oct 2001 15:58:08 -0500
Subject: Post-doc opening - LM, TEM & SEM

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POSTDOCTORAL POSITION IN MUCOSAL IMMUNOLOGY

Applications for an NEI-supported post-doctoral position to study M
cells in the mammalian conjunctiva using LM & EM techniques are being
accepted.

M cells are classically found overlying the mucosal lymphoid
follicles (Peyer's Patches) of the intestinal tract. The importance
of M cells in the mucosal immune response has only recently been
appreciated. M cells have a distinctive morphology that includes an
invagination of their basolateral membrane to form a "pocket" filled
with macrophages, lymphocytes and other antigen presenting cells.
The apical portion of the M cell forms thin bridges that separate the
extracellular world from the underlying lymphoid cells. This narrow
bridge can result in the distance from the apical membrane to the
underlying pocket of less than 2 microns. The M cell acts as an
antigen sampling cell by capturing souble and particulate lumenal
antigens, transcytosing them across the thin cytoplasmic bridge) and
releasing them into the subcellular pocket filled with macrophages
and other antigen presenting cells. Endocytosis of antigens by M
cells is thought to be the first step in generating a mucosal immune
response. There is considerable interest in the development of
immunogens targeted to M cells to develop immunity against diseases
such as cholera. It has also been realized that opportunistic
pathogens (e.g., HIV, Salmonella, Shigella) selectively bind to
intestinal M cells and exploit the transcytosis process to allow them
to penetrate the epithelial barrier.

M cells have also been found to occur in tonsils, bronchi, and the
nasal cavities. The presence of M cells in the ocular conjunctiva is
much more controversial. In collaboration with Dr. Cecil Moore, DVM
(a veterinary ophthalmologist and chair of Veterinary Medicine &
Surgery, MU Veterinary School), my lab has recently found LM, TEM,
and SEM evidence of cells with the highly distinctive morphology of M
cells in the conjunctiva of dogs (paper under review). Another group
found similar views in the Guinea pig. The bulk of the literature
claims M cells don't exist in the eye. This is mostly because no one
has ever tested the ability of follicular associated epithelium in
the eye to selectively transport antigens. Our new grant is designed
to test the ability of these putative M cells to selectively bind and
transport antigens of pathological importance. We intend to screen
the conjunctiva of multiple mammalian species to prove the widespread
existence of conjunctival M cells. We will also be testing whether
ocular immunization is superior to immunization at other sites in
regards to generating an ocular immune response. Candidates should
have experience in light and/or electron microscopy.

The project is mostly LM, TEM and SEM with some immunology later on.
Funding for the postdoc is $28,260 plus full benefits. There is
funding for 3 years but departmental policy requires offers to be
made for 1 year with re-appointment for subsequent years dependent on
satisfactory progress. The postdoc is available immediately but I
would be willing to wait a reasonable amount of time for the right
candidate. Send CV and names of three references by e-mail to
PhillipsT-at-missouri.edu.
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Tue Oct 16 18:10:08 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 16 Oct 2001 16:02:14 -0700
Subject: Looking for info & feedback on x-ray system

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

I am seeking feedback--good and bad--about a planned
x-ray detector system purchase.

I'm doing semiconductor work and need light element
detection (down to B). I do not have LN2. As a result,
I've narrowed the candidates to Noran's cryo cooled
10mm detector and their Quest software system. The
detector would be mounted on an Amray 1910 FESEM.

The Noran system comes with its own PC. I'd rather
have it integrated into my SEM control PC. But I suppose
this would work out OK in the long run. Noran uses
a Compaq PC with dual Ethernet ports. One port talks
to the pulse counter and detector interface. I may
try to use the other Enet port to connect the SEM PC.

I would appreciate any feedback from current users
of this product. I'm wondering about reliability and
up-time. Also, how are these systems warranted and
maintained? Off-list responses are welcomed.

Thanks,
gary g.



From daemon Tue Oct 16 18:15:13 2001



From: 01151938-at-mrc.vic.edu.au
Date: Wed, 17 Oct 2001 09:09:54 +1000
Subject: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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---
01151938-at-mrc.vic.edu.au
http://www.mrc.vic.edu.au/

Dear Listers
I am presently undetaking an Honours degree at La Trobe University, Victoria, Australia.I have been researching flower pollen using a Scanning Electron and Confocal microscopes.
I am particularly interested to hear your views on the following question:
Do you see a link between science and art? If so, how and why?
Thank you for considering my question.
Yours sincerely
Judi Bowden
La Trobe University
Victoria
Australia


From daemon Tue Oct 16 19:17:43 2001



From: j.miyan-at-umist.ac.uk ()
Date: Tue, 16 Oct 2001 19:07:52 -0500
Subject: Ask-A-Microscopist: stain for proteoglycans

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (j.miyan-at-umist.ac.uk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday,
October 16, 2001 at 12:07:24
---------------------------------------------------------------------------

Email: j.miyan-at-umist.ac.uk
Name: Jaleel Miyan

Organization: UMIST

Education: Graduate College

Location: Manchester, UK

Question: We are looking for a method to stain for proteoglycans in
sections of rat brain. I had heard that you can use a critical
eletrolyte method using alcian blue and varying magnesium
concentrations.

Can you direct me to a protocol and/or comment on the accuracy of
this and any other possible method.

MAny thanks

Jaleel

---------------------------------------------------------------------------


From daemon Tue Oct 16 22:56:04 2001



From: MARK JEFFREY TALBOT :      mark.talbot-at-studentmail.newcastle.edu.au
Date: Wed, 17 Oct 2001 13:47:08 +1000
Subject: Congo Red

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Hi everyone,

This is a reply to all the people who have been asking me about what
kind of success I have had with the cell wall dyes.

First of all, I am trying to stain 1 micron thick sections of LR
White-embedded cotyledons of Vicia faba. I got really nice staining with
a 1% solution (I know - it's pretty strong!) for 2 min (no heating). I
washed the sections after staining in running water for 15 min to remove
any unbound stain. The stain instantly got weaker when excited (green
filter) if I mounted the sections in water or 50% glycerol. So I mounted
them in Zeiss immersion oil, which gave me a really, really nice clear
image. The only problem was that, as was pointed out by Susan Joa, the
dye binds to 1-4 Beta Glucans, so the starch grains were fluorescing
pretty strongly. However, in my opinion the image I got was much better
than any calcofluor image I have ever taken. In addition, the nuceli
were lightly stained (does anyone know why this is?).

Sorry for the delay in answering yuor questions.

Mark Talbot.



From daemon Wed Oct 17 00:09:38 2001



From: Peter Jordan :      emsi-at-pe.net
Date: Tue, 16 Oct 2001 21:57:22 -0700
Subject: Zeiss 10C

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Hi:
We are looking (again) for a used Zeiss 10 TEM for the Los Angeles area.
Please let me know if you have one for sale or if you know of somebody
selling.
Thank you,
Peter Jordan
909 302-9130



From daemon Wed Oct 17 07:13:04 2001



From: mryder-at-brookes.ac.uk ()
Date: Wed, 17 Oct 2001 07:00:28 -0500
Subject: Ask-A-Microscopist:LM color changes in photographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mryder-at-brookes.ac.uk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
October 17, 2001 at 05:31:30
---------------------------------------------------------------------------

Email: mryder-at-brookes.ac.uk
Name: Michelle Ryder

Organization: Oxford Brookes University

Education: Graduate College

Location: Headington Campus, Gipsy Lane, Oxford, OX3 0BP

Question: Why do my wholemount lowish power root
photographs sometimes come out yellow, on the
same film, with the same lighting. Sometimes
the same root taken a few minutes later will
be yellow when it was the usual white on a previous frame.



---------------------------------------------------------------------------


From daemon Wed Oct 17 07:37:56 2001



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 10/16/01 3:05 PM
Subject: multiple specimen holder for Hitachi

Contents Retrieved from Microscopy Listserver Archives
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After my company bought a 3000N, I recognized that the Hitachi sample
holder system was relatively expensive. So I designed a 50 mm holder
with space for 8 12-13 mm dia. relatively inexpensive Zeiss stubs and
had a local machine shop make 7 of them. That included set screws for
the sample stubs and an appropriate center hole to screw in a Hitachi
base. The 3000N memory system was used to remember the centerpoint of
each of the 8 sample sites so moving from sample to sample is easy.
The cost was easily recovered considering the high cost of the Hitachi
mount system. All of the users were assigned a holder and it's worked
without a hitch ever since.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We were wondering if anyone else has run into this problem: we bought a
Hitachi VP S-3000N SEM. They offer a 125 mm multiple specimen holder -
essentially an aluminum disc on a small column that has 33 holes in it to
hold 33 stubs. We bought it and have now discovered that none of the
commercial vendors sell pin-type stubs that will actually fit into these
holes! The majority of stubs have pins that are 3mm in diameter. The holes
are only 2 mm in diameter. EMS can make the stubs at 13 cents a piece but
the minimum order is 100,000. Has anyone out there found a source of stubs
at a reasonable price (Hitachi offered to sell them at approximately $25.00
a piece!) or are people just drilling the holes in the holder to make them
bigger? I'd really like to hear how other people are handling this!

Lesley



Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191






From daemon Wed Oct 17 10:00:48 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Wed, 17 Oct 2001 10:52:28 -0400
Subject: Interaction between art and science

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Dear Judy,

While I'm not a microscopist, I think there is a strong link in that some of
the work one does for science can certainly become art.

If you are interested in further exploring the connection, I suggest you
contact David Scharf of Los Angeles CA. His email address is
dscharf-at-pacbell.net. David is an Artist who produces color SEM
photomicrographs and his work is outstanding. In fact, he recently won an
EMMY award for his work on a National Geographic documentary film called
"Body Snatchers".

Sincerely,

Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: "01151938-at-mrc.vic.edu.au"-at-sparc5.microscopy.com
[mailto:"01151938-at-mrc.vic.edu.au"-at-sparc5.microscopy.com]
Sent: Tuesday, October 16, 2001 7:10 PM
To: Microscopy-at-sparc5.microscopy.com



---
01151938-at-mrc.vic.edu.au
http://www.mrc.vic.edu.au/

Dear Listers
I am presently undetaking an Honours degree at La Trobe University,
Victoria, Australia.I have been researching flower pollen using a Scanning
Electron and Confocal microscopes.
I am particularly interested to hear your views on the following question:
Do you see a link between science and art? If so, how and why?
Thank you for considering my question.
Yours sincerely
Judi Bowden
La Trobe University
Victoria
Australia




From daemon Wed Oct 17 10:21:52 2001



From: Tom Doman :      jtd1-at-psu.edu
Date: Wed, 17 Oct 2001 10:14:22 -0500
Subject: Cell pellet processing for TEM

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Dear Colleges,

Is there a procedure for keeping a pellet of cells together (as
an intact pellet) while processing it for thin sectioning? I am working
with virus infected cells removed from the substrate via trypsin and
desire to keep the pellet densely packed and intact for subsequent
processing steps. The embedded pellet will then be thin sectioned.
Thanks in advance.

Tom Doman
Animal Diagnostic Laboratory
Penn State University


From daemon Wed Oct 17 11:15:05 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 17 Oct 2001 10:58:15 -0500
Subject: Epi-fluorescence on a stereoscope??

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I know they sell epi-fluorescence attachments for stereoscopes but I
am wondering how good they are in a practical sense. I want to
detect clusters of FITC-tagged bacteria clustered on large pieces of
mucosa measuring approximately 2 x 4 cm. Does anyone out there have
any experience in this type of work. TIA, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Wed Oct 17 11:15:05 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 17 Oct 2001 11:47:06 -0400
Subject: Re: Ask-A-Microscopist: stain for proteoglycans

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}
}
} Email: j.miyan-at-umist.ac.uk
} Name: Jaleel Miyan
}
} Organization: UMIST
}
} Education: Graduate College
}
} Location: Manchester, UK
}
} Question: We are looking for a method to stain for proteoglycans in
} sections of rat brain. I had heard that you can use a critical
} eletrolyte method using alcian blue and varying magnesium
} concentrations.
}
} Can you direct me to a protocol and/or comment on the accuracy of
} this and any other possible method.
}
} MAny thanks
}
} Jaleel
}
} ---------------------------------------------------------------------------
Jaleel
There are a variety of cationic dyes, which because of their charge,
will bind to proteoglycans, and that are electron dense. Many moons
ago, I used alcian blue, ruthenium red, safranin O, and
cetyl-pyridinium chloride (?) to contrast the ECM in heart muscle
(see Robinson, et al, lab. Invest. 49(4):482-97 1983.) Also, in the
1970's and 1980 the Simionescu's had a lot of publications using
various cationic dyes.

good luck,
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Oct 17 12:39:25 2001



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 17 Oct 2001 13:30:58 -0400 (EDT)
Subject: Re: Cell pellet processing for TEM

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Hello Tom,

You can resuspend your cell pellet in a few drops of 22 percent bovine
albumin prior to glutaraldehyde fixation. Then, when you add the glut.,
the BSA proteins will crosslink and become a solid chunk which can then be
processed as a piece of tissue.

An alternate method that I use is to resuspend the pellet and then
centrifuge at each step of the prep. I routinely process virally infected
cell monolayers this way. I like to embed in Spurrs rather than Epon
because of its lower viscosity, allowing for easier pelleting. I do not
use trypsin to lift the cells. Rather, I fix and post-fix the cells right
in the culture dishes. Then, after the osmium rinses, I scrape the
monolayers off of the dishes and pellet them in Eppendorf tubes for the
remainder of the process.

I hope this helps!

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
FAX (734)763-1166
dsoren-at-umich.edu

On Wed, 17 Oct 2001, Tom Doman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleges,
}
} Is there a procedure for keeping a pellet of cells together (as
} an intact pellet) while processing it for thin sectioning? I am working
} with virus infected cells removed from the substrate via trypsin and
} desire to keep the pellet densely packed and intact for subsequent
} processing steps. The embedded pellet will then be thin sectioned.
} Thanks in advance.
}
} Tom Doman
} Animal Diagnostic Laboratory
} Penn State University
}



From daemon Wed Oct 17 13:33:53 2001



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Wed, 17 Oct 2001 11:23:55 -0700
Subject: Re: Cell pellet processing for TEM

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleges,
}
} Is there a procedure for keeping a pellet of cells together (as
} an intact pellet) while processing it for thin sectioning? I am working
} with virus infected cells removed from the substrate via trypsin and
} desire to keep the pellet densely packed and intact for subsequent
} processing steps. The embedded pellet will then be thin sectioned.
} Thanks in advance.
}
} Tom Doman
} Animal Diagnostic Laboratory
} Penn State University

After fixing, pellet it down in the buffer in an eppendorf tube
remove the buffer and add a drop of low melting point agarose made up
in buffer (add no more than the same size of pellet)
use a warmed pin (37 degrees) or long needle to swirl the pellet into
the agarose before it sets.
When set use a razor blade to cut off the end of the eppendorf to get
at the pellet
poke out the pellet onto a drop of buffer on dental wax or parafilm
chop the agarose pellet into 1 mm squares
put into a scintillation vial and process as normal for tissue pieces.

If the layer of cells is almost invisible, cover with a tiny amount
of low melting point agarose
set by cooling
finish the processing in the eppendorf as long as the low melting
point agarose does not lift off the bottom of the tube but traps the
cells in place. In this case the thickness of the LMP agarose should
allow penetration of the chemicals so should be very thin.

Elaine

--
Dr. Elaine Humphrey
Director, Biosciences Electron Microscopy Facility
First Vice President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca
website: www.emlab.ubc.ca


From daemon Wed Oct 17 14:39:00 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 17 Oct 2001 15:32:09 -0400 (EDT)
Subject: Re: Cell pellet processing for TEM

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Remove the cells from the substrate (if you're dealing with an enveloped
virus, you might want to use means other than trypsin).

Pellet cells gently (2000 x g for 10 min)

Resuspend in glut in microfuge tube.

Swirl for 30 sec, then pellet again.

Let pellet sit without resuspending to fix. Time will depend on pellet size.

After 1 to several hours, remove fix with pipet; cut end off plastic tube
with razor blade. Holding tip with forceps under dissecting, scope scoop
out pellet with flattened and pointed applicator stick.

Dry further with pie-shapped wedge of filter paper by touching only the
tip to the cell mound. The consistency should be that of cooked
oatmeal. If the pellet is not dry enough, cells will disperse in the
agar and be hard to find in the EM. If it is too dry, ultrastructure
will suffer.

Divide the pellet into clumps about 1 mm cubed with a razor blade or
spatula and surround them with molten agar*.

Let harden, and wash in buffer. Proceed with osmium, etc., as though you
had tissue blocks.

*Mix 1 g purified agar with 100 ml water (or 0.1 g in 10 ml); melt by
boiling--watch out, it easily boils over. Put into small tubes; cap or
cover with Parafilm. Store at 4 degrees C. When needed, melt in beaker
of boiling water. Let cool till barely warm to touch--don't cook the
cells. (Agar solidifies at about 36 degrees and melts at about 95 degrees.)

Do not put agar-encased cells into glut. It will cross-link the agar so
that subsequent solutions don't penetrate. However the remaining glut in
the (unwashed) cells prior to encasing in agar won't hurt.


Good luck.

Sara Miller


On Wed, 17 Oct 2001, Tom Doman wrote:

} Date: Wed, 17 Oct 2001 10:14:22 -0500
} From: Tom Doman {jtd1-at-psu.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Cell pellet processing for TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleges,
}
} Is there a procedure for keeping a pellet of cells together (as
} an intact pellet) while processing it for thin sectioning? I am working
} with virus infected cells removed from the substrate via trypsin and
} desire to keep the pellet densely packed and intact for subsequent
} processing steps. The embedded pellet will then be thin sectioned.
} Thanks in advance.
}
} Tom Doman
} Animal Diagnostic Laboratory
} Penn State University
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Oct 17 14:39:07 2001



From: Lilly M. :      lilly-at-cycy.net
Date: Wed, 17 Oct 2001 12:30:24 -0700
Subject: High vacuum on Hitachi H600

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Hi everyone,

We are having a problem with the high vacuum on our HItachi H600 TEM. When the microscope is
first turned on, the high vacuum is achieved but about 5 to 10 minutes later it starts
dropping. The problem developed over time. In the beginning, it would drop by very little and
go back up again. Later on, it started dropping by a lot more and it took longer to get back
up again. Now, the vacuum is almost competely lost. Does anybody know what might be causing
this problem? Any tips or suggestions? We appreciate the help and thank you all in advance.


From daemon Wed Oct 17 14:55:19 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Wed, 17 Oct 2001 12:48:25 -0700
Subject: science and art

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I am a little rusty on this, but when I did my masters in interdisciplinary arts, I used the SEM. The connection I used between science and art was creativity. There are many fields that we can be creative in...science and art are two of them. We consider art the mother of creativity. Yet, we live in a scientific/technological paradigm these days more than a religious paradigm like the Middle Ages. Those main religious and scientific paradigms overlap(think of interlapping rings much like the Olympic rings) into other disciplines like medicine, art, history, and law. The thread that keeps it all woven together is creativity. There are many definitions to clarify, but I believe looking at science and art as part of a bigger construct fueled by creative energy will help you with the larger picture. The more immediate picture like what is science and what is art and what is creative remains for us to define and redefine. How we define it is by the tools we use, the message we seek, the role of transformation and discovery in what we produce. Good Luck!



From daemon Wed Oct 17 15:17:15 2001



From: gerard.d.gagne-at-abbott.com
Date: Wed, 17 Oct 2001 15:11:22 -0500
Subject: Re: Cell pellet processing for TEM

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Tom,

We do this all the time with cell suspensions. Pellet the live cells (~1,000
rpm for 10 minutes), carefully decant the supernatant and add cold 3%
glutaraldehyde, being careful not to disturb the pellet. After about an hour
of fixation, you should be able to dislodge the pellet and process it as if it
were a piece of tissue.

Hope this helps.

Jerry Gagne
Senior Biologist
Department of Microscopy and Microanalysis
Abbott Laboratories
(847) 938-5023



From daemon Wed Oct 17 18:13:08 2001



From: Allan Mitchell :      allan.mitchell-at-stonebow.otago.ac.nz
Date: Thu, 18 Oct 2001 12:07:18 +1300
Subject: TEM of cadaver tissue

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Kia Ora all

We have a student starting to work with human cerebral blood vessels
obtained from cadavers.

A literature search has revealed little information on working with
cadaver material at the TEM level.

The questions from this student are;

1. Does anyone know of any publications resulting from TEM
investigations of samples from human cadavers, in particular cerebral
blood vessels and brain / nervous tissue ?


2. The standard EM fixation texts talk about not using formalin to
fix for TEM and emphasize speed of primary fixation. My initial
results indicate that the ultrastructure of the cadaver cerebral
blood vessels has remained unexpectedly intact.

Has anyone else experienced good ultrastructural results in the TEM
with cadaver material, embalmed in 10% Neutral Buffered Formalin and
after an initial post mortem delay of up to 24 hours ?


- What ultrastructural changes (at the organelle level) did you
expect and then what actual ultrastructural changes did you see ?


3. What ultrastructural changes would you expect from such material
if it has been in 10% Neutral Buffered Formalin for up to 6 months
prior to processing and examination ?

On behalf of our student, thanks for your help.

Allan


--
-------------------------------------------------
Allan Mitchell
Technical Manager
Otago Electron Microscope Centre
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 7254

Unit: http://www.otago.ac.nz/anatomy/emunit/
Department: http://anatomy.otago.ac.nz/



"Life is a gift, don't waste it"


From daemon Wed Oct 17 18:13:08 2001



From: Cheng Huang :      HUANG-at-rsbs.anu.edu.au
Date: Thu, 18 Oct 2001 12:07:23 +1000
Subject: Cryo-attachment (FC 4) for Reichert-Jung ultramicrotome

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----- Original Message -----
} From: {mryder-at-brookes.ac.uk}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, October 17, 2001 8:00 AM


Dear Listers,

We have recently got a free set of cryo-attachment (FC 4) for our old Reichert-Jung ultramicrotome. However, there are two parts missing. One is the alloy steel bridge, which connects the specimen to the ultramicrotome's specimen arm. The other is the steel guide rails, which are used for the mounting of the cryo-chamber. Anyone has these parts or whole cryo-attachment to give away or sell? Please let us know.

Thanks
Cheng

-----------------------------------------------------
Cheng Huang
Australian National University
EM Unit, RSBS
Box 475, ACT 2601
Canberra, Australia
Phone: 61-2- 6125-6553
Fax: 61-2- 6125-3218
http://www.anu.edu.au/EMU/




From daemon Thu Oct 18 02:39:22 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 18 Oct 2001 17:32:14 +1000
Subject: RE: High vacuum on Hitachi H600

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Is your cooling water cool on return?
How long since your dif pump was cleaned and recharged?
Vacuum fluid can diminish over time and when its down to half of the initial
charge you may have that type of problem. Fluid will work well in some TEMs for
years, but suspect the pump if its more than two years since the last fluid
change - and less if the pump lived through a couple of mishaps.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, October 18, 2001 5:30 AM, Lilly M. [SMTP:lilly-at-cycy.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi everyone,
}
} We are having a problem with the high vacuum on our HItachi H600 TEM. When
} the microscope is
} first turned on, the high vacuum is achieved but about 5 to 10 minutes later
} it starts
} dropping. The problem developed over time. In the beginning, it would drop
} by very little and
} go back up again. Later on, it started dropping by a lot more and it took
} longer to get back
} up again. Now, the vacuum is almost competely lost. Does anybody know what
} might be causing
} this problem? Any tips or suggestions? We appreciate the help and thank you
} all in advance.


From daemon Thu Oct 18 04:41:31 2001



From: Dionezie Bojin :      bojin-at-mail.physics.pub.ro
Date: Thu, 18 Oct 2001 12:39:21 +0300
Subject: Application for SEM, TEM, AFM

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Hi, everyone,


My colleague Dr. I. Pencea has a proposal for an aplication, if
someone is interested in this please contact us.

Best regards,
Prof. D. Bojin

"
Dear Sir/Madam,

My name is Ion Pencea and I am Assoc. Professor with "Politehnica
" of Bucharest, Romania, Materials Science & Engineering Faculty.
My research field is materials characterization by WAXD, SAXD and XPS.
I have gained a scholarship for training in microscopy
investigations (SEM, TEM, AFM, etc.). The training period is of least 6
month and maximum 12 month depending on host agreement. Thus, I am looking
for a laboratory which could accept me as a visiting scholar or other
position (post doc., etc.).
I have some experience in SEM and TEM with a TESLA microscopes (BS
350, BS 540) but I am interested in a modern SEM with EDS / WXD
attachments, and of course in TEM and AFM.
During this training period I can help with investigation on
materials of host interest and other specific work (documentation, papers,
reports, etc.).
My scholarship will cover my accommodation but if it is possible
to get work permission it will be perfect.
If you are interested in my application, please contact us.


Sincerely yours,
Dr. I. Pencea
Hi everyone,






From daemon Thu Oct 18 08:21:00 2001



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Thu, 18 Oct 2001 11:48:24 -0230
Subject: art and science

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Most Hitachi microscopes have a seleniod valve that "vents" air into the
vacuum line when the SEM/TEM is turned off. This prevents any residual
vacuum from sucking oil back into the forlines and causing startup problems.

This seleniod valves get really hot during operation and, over the years,
causes the hose to crack.
This small crack allows air into the foreline thereby raising the foreline
pressure & ultimately the SEM/TEM pressure. As the crack gets larger, the
foreline pressure is worse, etc.

The first thing to do is to identify the Selenoid valve(s) in question.
Trace the vacuum lines from the rotary pumps to a "T" in the line. The "T"
should have a 3/8 in (6mm) OD rubber hose coming from the "T" to the top of
the solenoid valve. The solenoid valve will be very hot. Either replace the
hose or cut it shorter depending upon it's condition.

Hope this Helps.

Earl Weltmer
Scanservice Corp.


----- Original Message -----
} From: "Lilly M." {lilly-at-cycy.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, October 17, 2001 12:30 PM


Judi Bowden writes ...

} ...
} Do you see a link between science and art? If so, how and why?
} ...

I think you are mixing apples and oranges. "Science" is a process by
which we learn ... "art" is something we create and/or observe. However,
that is not to say we cannot use a scientific process to learn how to
create, or create differently.

my 0.02 ... shAf :o)



From daemon Thu Oct 18 09:58:02 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 18 Oct 2001 10:50:54 -0400 (EDT)
Subject: Re: TEM of cadaver tissue

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We have examined numersous brain samples, both biopsy and autopsy
material. The damage you will see due to autolysis will totally
obliterate any difference you might have seen due to the use of formalin
versus or prior to glutaraledehyde. Length of time storaged in fix will
also affect the tissue less that length of time the person was dead before
the tissue went into fixative.

The best ultrastructure would be obtained on biopsy material (hard to
get), and the next best structure would be from rapid autopsies, like
those done in 30 min after Alzheimer's Disease patients have had died for
the special studies some folks are doing on rapidly decaying processes.

If one is comparing ultrastructure of one case to another, (s)he needs to
note carefully the length of time the person was dead before the tissue
went into fix (or the person was embalmed). This time can vary widely.

Sara Miller


On Thu, 18 Oct 2001, Allan Mitchell wrote:

} Date: Thu, 18 Oct 2001 12:07:18 +1300
} From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz}
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM of cadaver tissue
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Kia Ora all
}
} We have a student starting to work with human cerebral blood vessels
} obtained from cadavers.
}
} A literature search has revealed little information on working with
} cadaver material at the TEM level.
}
} The questions from this student are;
}
} 1. Does anyone know of any publications resulting from TEM
} investigations of samples from human cadavers, in particular cerebral
} blood vessels and brain / nervous tissue ?
}
}
} 2. The standard EM fixation texts talk about not using formalin to
} fix for TEM and emphasize speed of primary fixation. My initial
} results indicate that the ultrastructure of the cadaver cerebral
} blood vessels has remained unexpectedly intact.
}
} Has anyone else experienced good ultrastructural results in the TEM
} with cadaver material, embalmed in 10% Neutral Buffered Formalin and
} after an initial post mortem delay of up to 24 hours ?
}
}
} - What ultrastructural changes (at the organelle level) did you
} expect and then what actual ultrastructural changes did you see ?
}
}
} 3. What ultrastructural changes would you expect from such material
} if it has been in 10% Neutral Buffered Formalin for up to 6 months
} prior to processing and examination ?
}
} On behalf of our student, thanks for your help.
}
} Allan
}
}
} --
} -------------------------------------------------
} Allan Mitchell
} Technical Manager
} Otago Electron Microscope Centre
} C/-Department of Anatomy and Structural Biology
} School of Medical Sciences
} P.O. Box 913
} Dunedin
} New Zealand
}
} Phone (03) 479 5642 or 479 7301
} Fax (03) 479 7254
}
} Unit: http://www.otago.ac.nz/anatomy/emunit/
} Department: http://anatomy.otago.ac.nz/
}
}
}
} "Life is a gift, don't waste it"
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Oct 18 10:30:48 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 18 Oct 2001 08:32:19 -0700 (PDT)
Subject: Re: science and art

Contents Retrieved from Microscopy Listserver Archives
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Judy,
For most of us, it isn't good enough just to take a picture that shows what
we need to see, it also has to look good. I think that is where the art
comes in.
I've had 3 cover micrographs for the Scanning Journal
(www.scanning-fams.org).Most of the images were from my Ph. D.
research, but add some color...and you have ART!

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112

----- Original Message -----
} From: {"01151938-at-mrc.vic.edu.au"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, October 16, 2001 6:09 PM



I heard a definition of art when I was in photography school many years
ago. I wish I knew the source...

Art is anything, that when abstracted from its surroundings, embibes its
viewer with a hieghtened or altered sense of thier own existence.

Soon after is when I went from fine art to scientific imaging because it
does this so magically.

Bob Underwood
U of W
Derm Imaging Center




From daemon Thu Oct 18 10:39:19 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 18 Oct 2001 11:32:07 -0400 (EDT)
Subject: Help with clinical EM procedures

Contents Retrieved from Microscopy Listserver Archives
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If you perform clinical TEM or SEM, I need your input on a College of
American Pathology (CAP) reevaluation of the labor time they allot for
these procedures. If you're willing to help, please contact me offline,
and I will send you a draft of the operating procedures for your comments.

The CAP has CPT codes that determine how much hospital labs can be
reimbursed. The descriptions for TEM and SEM procedures are out of
date and appear to have been written by a lab director who was not
actually an electron microscopist as some of the steps don't make sense.
I have been asked by CAP to submit updated lists for consideration for
adoption.

We prepare several hundred EM samples per year for Duke hospital, and I
have, with the help of my 5 superb techs, listed each step and the time
it takes to perform them. If you work in a hospital lab or are
contracted to perform EM on human tissue, I would very much appreciate
your input. Please contact me.

Thanks in advance,
Sara

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Oct 18 10:59:12 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 18 Oct 2001 08:50:04 -0700
Subject: Re: High vacuum on Hitachi H600

Contents Retrieved from Microscopy Listserver Archives
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Dear Lilly,
The two things that I know that can cause that are:
1. the heater on the diffusion pump is not quite heating hot enough to boil
the oil,
2. the diffusion pump oil is almost gone.
The fact that you achieve high vacuum means that your system is almost
working and has no leaks.
At 12:30 PM 10/17/01 -0700, you wrote:
}
} Hi everyone,
}
} We are having a problem with the high vacuum on our HItachi H600 TEM. When
the microscope is
} first turned on, the high vacuum is achieved but about 5 to 10 minutes
later it starts
} dropping. The problem developed over time. In the beginning, it would
drop by very little and
} go back up again. Later on, it started dropping by a lot more and it took
longer to get back
} up again. Now, the vacuum is almost competely lost. Does anybody know
what might be causing
} this problem? Any tips or suggestions? We appreciate the help and thank
you all in advance.
}
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 18 12:02:30 2001



From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Thu, 18 Oct 2001 09:49:52 -0700
Subject: art and science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a few opinions about this topic since I am a research microscopist
by day and an artist by night. One of my interests involves utilizing
elements from science in the production of art. I have an
exhibition/performance November 8th--24th at SomArts Gallery in San
Francisco that will include some exemplary pieces. You are invited! You can
learn more from the web site at http://SaintRubidium.com I will be adding
additional descriptions of the art work to the web site before November
1st. Some examples are: a screen made of 6000 Petri dishes; micro art
viewed with a light microscope and made with drosophila wings, human blood
and copper sulphate; a video of a 30 foot by 30 foot array of data tapes
from electrophysiology potassium channel recordings, an eight foot cylinder
"totem" made from science journal cover photos; another totem made from
radiographic films and transilluminated; a computer controlled
3-slide-projector with stereo sound presentation of botanical scanning
electron micrographs made by Douglas Frank in Portland, Oregon.

I find the world of science rich with imagery and fascinating materials
that are exciting to use in a manner that brings a different perspective on
science to the public while investingating personal issues as well as some
issues common to all of humankind and the universe. There are numerous
people interested in the intersection of art and science. YLEM just
celebrated their 20th anniversary with an excellent exhibit in San
Francisco. More information can be found at http://www.ylem.org See their
links page for connections to other people and organizations such as the
San Francisco Exploratorium. Another resource is the journal Leonardo / the
International Society for the Arts,Sciences and Technology. See:
http://mitpress2.mit.edu/e-journals/Leonardo/index.html

I hope that these few words explain a little bit of the how and why of the
connection between art and science. I must add, however, that I learned
early on in my career that there are scientists that find this whole art
interest in science repugnant. I'm still not clear why but can only
speculate. I also find that the influence of my art on my science is much
less clear to me except that it provides some motivation to survive the
trudgery that experiments often require. Larry



From daemon Thu Oct 18 12:13:44 2001



From: msdunn-at-pop.mailnara.net
Date: Thu, 18 Oct 2001 12:03:05 -0500
Subject: Harvest Nice Rewards With Knowledge/Experience 28035

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Oct 18 13:13:05 2001



From: Danowski, Kristine (KL) :      KLDanowski-at-dow.com
Date: Thu, 18 Oct 2001 14:04:25 -0400
Subject: RE: art and science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The American Chemical Society celebrates National Chemistry Week during
November 4-10, 2001. This year's theme is "Celebrating Chemistry and Art."
For more information, refer to http://www.acs.org and scroll down to
National Chemistry Week.

Kristine Danowski
Analytical Sciences
Dow Chemical Company
Midland, MI 48667
kldanowski-at-dow.com



From daemon Thu Oct 18 13:41:02 2001



From: Saul, Steven :      SSaul-at-optronics.com
Date: Thu, 18 Oct 2001 11:33:47 -0700
Subject: Definition of phrase used by co-worker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hello:
} Could somebody please define for me "home brew and ASR rules"? Does this
} phrase refer to how reagents or antibodies are made, in-house vs.
} commercially?
} Thanks,
} Steve Saul
}
} e-mail: ssaul-at-optronics.com


From daemon Thu Oct 18 14:02:17 2001



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Thu, 18 Oct 2001 11:54:24 -0700
Subject: re: cell pellet for TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Tom,
I have prepared cell pellets for virus-infected cells many times. If the cells are in 6-well plates, one can process them in the plates thru the dehydration and release the cells (quickly!) with washes of propylene oxide. The cells can then be infiltrated in a P.O. resistent polyproylene test tube and spin down after each change of resin. When embedding, then pipet off the resin to the pellet and tranfer the pellet to beem capsules. Spin them in a piggy-back arrangement with the beem capsule tucked in a microcentrifuge tube. (Cut the microcentrifuge tube so the beem capsule fits) Close the cap, spin and top off with resin.
If the cells are in suspension, one must spin after each step of fixation, dehydration, and infiltration before transfering them to beem capsules to embedd. But, you may process suspension cells in the polypropylene tubes which are easy to spin.

Barbara Plowman
Univ. of the Pacific
School of Dentistry
2155 Webster St. Rm 632
San Francisco, CA 94115
email: Bplowman-at-sf.uop.edu
phone: 415-929-6692



From daemon Thu Oct 18 14:22:33 2001



From: Ziegler, David SBCCOM(N) :      David.Ziegler-at-Natick.Army.Mil
Date: Thu, 18 Oct 2001 15:13:42 -0400
Subject: High vacuum on Hitachi H600

Contents Retrieved from Microscopy Listserver Archives
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Hi Lilly,

I operate an H600 and have seen a similar problem. First, by turned "on" do
you mean the high tension voltage (e.g.. 100kv) or do you mean physically
starting the system from no pumps running to switching on the pumps and
waiting for the DP's to heat up and pump the system down?

If you mean applying the 100kv, I've found that filling the large LN2 trap
before switching on the 100kv virtually eliminates any vacuum swings.

The other problem may be that you need to clean the PENNING gauge vacuum
sensor. if its heavily coated with junk it will short-out and you see it as
vacuum drops and poor levels on the readout. There is a section in the H600
instrument manual that tells you how to clean the gauge. Its very easy to
do and takes about 2-3 hours of your time. If needed please contact me off
list and I can fax you the pages in the manual.

dz

-----Original Message-----
} From: Lilly M. [mailto:lilly-at-cycy.net]
Sent: Wednesday, October 17, 2001 3:30 PM
To: Microscopy-at-sparc5.microscopy.com


Hi everyone,

We are having a problem with the high vacuum on our HItachi H600 TEM. When
the microscope is
first turned on, the high vacuum is achieved but about 5 to 10 minutes later
it starts
dropping. The problem developed over time. In the beginning, it would drop
by very little and
go back up again. Later on, it started dropping by a lot more and it took
longer to get back
up again. Now, the vacuum is almost competely lost. Does anybody know what
might be causing
this problem? Any tips or suggestions? We appreciate the help and thank
you all in advance.


From daemon Thu Oct 18 14:54:32 2001



From: Bruce Girrell :      bigirrell-at-microlinetc.com
Date: Thu, 18 Oct 2001 15:48:16 -0400
Subject: RE: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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Mike Nesta wrote:

} If you are interested in further exploring the connection, I suggest you
} contact David Scharf of Los Angeles CA... David is an Artist who produces
} color SEM photomicrographs and his work is outstanding.

The reason that I have an SEM is because of David Scharf. In 1976 I bought a
copy of his book _Magnifications_ and was entranced by the images. Scharf
clearly not only has mastery of the SEM, but also an artist's eye for
composition (and a sense of humor, as well). As I looked through the images
over and over, I came to believe that the SEM has to be one of the best toys
ever invented. It took 25 years, but I now have my own toy.

I believe that science and art support each other very well. One of my other
interests is pottery. My scientific background helps me understand which
forms will be mechanically strong and which will be fragile, what chemicals
in glazes do, how heat in the kiln works, how to conduct meaningful
experiments... I feel sorry for those who attempt to create pottery
_without_ the benefit of a scientific background. They are at a distinct
disadvantage.

Scientific endeavors can also benefit from art. My favorite example is (was)
O'Shaughnessy dam on the Scioto river near Columbus Ohio. I say "was"
because the original dam no longer exists. The dam was beautiful. It was not
just a rectangular block of concrete poured into the path of a river. It had
graceful curves and portions of it were terraced into the surrounding rock.
Rather than simply plummeting down a abrupt spillway, the water broke over a
curved edge, cascaded down the main portion of the breast, and was
reintroduced to the river with a reverse curve at the bottom. As a result,
the water fell almost silently. More sound came from the small terraced
portions, where the water might fall 5 or 10 feet at a time, than from the
main spillway. O'Shaughnessy served its purpose well as a dam and was
beautiful at the same time.

Bruce Girrell





From daemon Thu Oct 18 15:02:24 2001



From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 18 Oct 2001 15:56:17 -0700
Subject: Re: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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Hi Judy,
There was a great talk this year at the M&M Meeting in Long Beach, CA
related to that subject. The MSA Presidential Happenings lecture was
presented by Stuart S. Sumida from California State University San
Bernardino: From Bones and Stones to Balloons and 'Toons: Bringing Biology
and Art Together in Realistically Animated Films.
He's a paleontolgist and he has advised Disney animators on animal movement
for such films as The Lion King, Mulan, etc. He would be a great person to
contact. Perhaps someone has his email address?

On a personal note, I enjoy doing 35mm B&W macro photography. Last year I
entered photographs in a juried show. Up to 3 photos could be entered, so I
submitted 2 of my favorite 35mm photos and for a laugh I threw in a SEM of
pollen grains on a Bumblebee's back. Needless to say I was truly miffed
when the SEM was accepted and my others were rejected - ha! Anyway, it
made me think about my work and how I approach it. I do try to frame things
up in the most esthetically pleasing way and still have it contain all the
scientific information that's needed. Even though I've separated my work
from my art for many years, I think all microscopists are artists. Is
anyone else observing our world so closely? ...well, besides the media;-)
good luck with your project, Beth

} Dear Listers
} I am presently undetaking an Honours degree at La Trobe University,
} Victoria, Australia.I have been researching flower pollen using a Scanning
} Electron and Confocal microscopes.
} I am particularly interested to hear your views on the following question:
} Do you see a link between science and art? If so, how and why?
} Thank you for considering my question.
} Yours sincerely
} Judi Bowden
} La Trobe University
} Victoria
} Australia

******************************************************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
******************************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull. Aqualung

******************************************************************************om




From daemon Thu Oct 18 15:03:22 2001



From: efosten-at-mmm.com
Date: Thu, 18 Oct 2001 14:58:28 -0500
Subject: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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"Do you see a link between science and art? If so, how and why?"

Judi,

Linking creativity in art and science:

Creativity in art is not substantially different from creativity in
science. Creativity in art/science is not a person suddenly struck by a
blinding flash of inspiration/insight and then producing a masterpiece.
Even the most radical artists/scientists start from classical knowledge and
then spend years polishing their technique. [Picasso, Mozart, Einstein]
(source: Julio Ottino, lecturer on "Creativity ? an Art View", an
accomplished artist and Professor, and Chair, Department of Chemical
Engineering, Northwestern University, Evanston, Illinois, USA).

"Even the most talented persons do not reach world class performance in
their fields until they have devoted about 10 years or more of rather
single minded attention to becoming an expert". What chiefly distinguishes
creative thinking from more mundane forms [in art/science] are Ambiguity,
Persistence and Knowledge - (i) willingness to accept vaguely defined
problem statements and gradually structuring them, (ii) continuing
preoccupation with problems over considerable periods of time, and (iii)
extensive background knowledge in relevant and potentially relevant areas.
(source: H. Simon, Nat'l Acad Sci Proc, 80, 4569-4571, 1983).

Art is the selection from a great number of variables (source: book, Zen
and the Art of Motorcycle Maintenance). There are also a great number of
variables to select from at the start of a new science project.


Regards,


Ev Osten

efosten-at-mmm.com
651-736-0104
fax: 651-733-0648

3M Company
Corporate Analytical Technology Center
3M Center, 201-BE-16
St. Paul, MN 55144-1000
U.S.A.




From daemon Thu Oct 18 15:08:42 2001



From: Gay R. Holstein :      holstg01-at-doc.mssm.edu
Date: Thu, 18 Oct 2001 13:27:25 -0400
Subject: reply to listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


the address is correct.




From daemon Thu Oct 18 15:16:28 2001



From: Mark Riggs :      Mark.Riggs-at-asml.com
Date: Thu, 18 Oct 2001 16:10:44 -0400
Subject: semming a capacitor

Contents Retrieved from Microscopy Listserver Archives
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just heard a phone message inquiry about the ability of a sem to evaluate "micro-cracking" of a capacitor's outer shell. the requestor believes the "micro-cracking" might be indicative/source of performance problems. is this possible, and how do i sem it? mr



From daemon Thu Oct 18 16:28:32 2001



From: David Barnard :      David.Barnard-at-wadsworth.org
Date: Thu, 18 Oct 2001 17:19:15 -0400
Subject: art and science

Contents Retrieved from Microscopy Listserver Archives
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From my background in physics I find this question very intriguing.

Art stands out by its resonance with our experience or by harmony
with something within us. We marvel at the expression. Sometimes it
expreses a play on patterns with nothing to do with function like
designs on ancient potery while other times it's the expression of
functional form, the bowmans weapon or the flying gull. We identify,
at once the ideal human form in the Greek sculptures and dynamic
figures from the caves in France while we see the vision and mastery
of the artist.

With science it may not be so different. We paint with a bigger brush
with a handle of technology, bristles of training, and paint of
intellegent abstractions on a canvas of hypothetical vision. Does not
this or that stroke of the brush resemble the observation? If not
quite, then improve the stroke, refine the correspondance. Generalize
the circle to an ellipse. Now the planetary cycles correspond... The
Painting is never done. Maybe it is more like an evolving building
with not so many master archetects and many builder craftsmen all who
live in the structure they build daily. Their dedicated methodology
is required to keep the structure sound with each new change.

But science is an art. It is an art of knowing, a craft of many
participants. Mere scientific method cannot make breakthrough
discoveries. It is an art of creative nurturing of insight correctly
applied to each step.

The same honesty requred in the scientific method may also be
required by any artist to acheive her mastery of technique.. The same
aesthetic experience of the artist and viewer may also be part of the
experience of scientist and student.


My $00.04 worth,

Dave
--
David Barnard
Wadsworth Ctr
NYS Dept Health
Albany NY 12201-0509
barnard-at-wadsworth.org
518 473-5299 voice
518 474-7992 fax


From daemon Thu Oct 18 16:55:14 2001



From: Dan Bumbarger :      danbu-at-citrus.ucr.edu
Date: Thu, 18 Oct 2001 14:54:20 -0700
Subject: Dirty Nematodes

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I am trying to do some SEM on nematodes with elaborate structures on
their front ends. Unfortunately, these critters secrete some sort of
sticky residue around these structures that makes it difficult to get
good, clean specimens. The "stuff"is likely some sort of glycoprotein
goo and I should (hopefully) be able to rinse it off with something,
without damaging the integrity of the collagenous cuticle and without
giving me other sorts of artifacts. Any ideas?
--
______________________________________________
Dan Bumbarger
Graduate Student
Depts. of Biology and Nematology
University of California, Riverside
Riverside, CA 92521
______________________________________________


"With a little more deliberation in choice of pursuits, all men would
perhaps become essentially students and observers, for certainly
their nature and destiny are interesting to all alike. In
accumulating property for ourselves or our posterity, in
founding a family or a state, or acquiring fame even, we are
mortal; but in dealing with truth we are immortal, and need fear no
change nor accident."


-H.D. Thoreau, "Walden"



From daemon Thu Oct 18 18:58:21 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 18 Oct 2001 19:48:37 -0400
Subject: RE: Ask-A-Microscopist:LM color changes in photographs

Contents Retrieved from Microscopy Listserver Archives
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Dear Michelle,
Had the same problem when I was starting out. It turned out that I
was alternating with someone who was using tungsten film and removed the
daylight filter for his photography. On another occasion when I was the
lone user, I found that there were lots of voltage changes during the day
and photos taken at one time were different from those taken at another.
The immediate way to handle the voltage problem is to check the power supply
voltage level for every exposure.
When I had a class taking photos, I used a tabular sign-in sheet
with columns for: Power supply setting; Iris diaphragm open; field
diaphragm adjusted; objective; time of photo; trinocular prism setting (1,2,
or 3); etc. I think you get the point. If your line voltage is at fault,
the only way you can prove it is to have someone perform a time study to
record those changes over a 24hr period at your outlet.
The next problem that I have seen is caused by picking a roll of
film on Monday that is designated for use with a tungsten source and another
roll on Thursday that is designated for use with daylight.
Oh, yes! AND FINALLY, if your photo detector is set for spot
metering rather than averaging, a small change in location can/will have a
great effect on the result, which will appear to be unpredictable.
From all of the above, your best bet is the proper compensating
filter when using artificial light (LBD (Olympus) for daylight-type(5500K)
and LBT for tungsten-type(3400K) films)
For years I have treasured a book I acquired from Olympus entitled,
"How To Improve Photography Through the Microscope" (Part # M132E-0886T).
There is another that I acquired from Leitz before it became Leica.

Hope that one small part of this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


} ----------
} From: mryder-at-brookes.ac.uk
} Sent: Wednesday, October 17, 2001 8:00 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist:LM color changes in photographs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mryder-at-brookes.ac.uk) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} October 17, 2001 at 05:31:30
} --------------------------------------------------------------------------
} -
}
} Email: mryder-at-brookes.ac.uk
} Name: Michelle Ryder
}
} Organization: Oxford Brookes University
}
} Education: Graduate College
}
} Location: Headington Campus, Gipsy Lane, Oxford, OX3 0BP
}
} Question: Why do my wholemount lowish power root
} photographs sometimes come out yellow, on the
} same film, with the same lighting. Sometimes
} the same root taken a few minutes later will
} be yellow when it was the usual white on a previous frame.
}
}
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Thu Oct 18 19:06:03 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 18 Oct 2001 19:59:14 -0400
Subject: Re: Art and Science, Science and Art

Contents Retrieved from Microscopy Listserver Archives
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"This method did not work in our hands." "I've been looking at histologic
sections for 20 years, and I never made that connection." "How did you get
these preparations to have such low background?" If there is no art in the
application of the principles of science to the business of discovery, then
there is no discovery.

Here's to the art(full) scientist who discovers what others have missed,
because she sees the truth in corn while everyone else is treating it like
food.

Regards to all,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Thu Oct 18 19:07:06 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 18 Oct 2001 20:03:26 -0400
Subject: Re: High vacuum on Hitachi H600

Contents Retrieved from Microscopy Listserver Archives
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Lilly,
I've seen this kind of behavior if the oil level in the diffusion pump
gets low (I'm assuming that it is diffusion pumped). What happens is
that the oil starts to boil and the DP pumps. When the oil is all
boiled off, it stops pumping until some condensed oil runs down the side
and back into the boiler section where it can boil and pump a little more.

The solution is to remove the DP, clean it and recharge it with the
proper amount of the proper oil. Generally this problem occurs when you
either have a large air leak or the DP is frequently vented when still
quite hot, a definite no-no.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


Lilly M. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi everyone,
}
} We are having a problem with the high vacuum on our HItachi H600 TEM. When the microscope is
} first turned on, the high vacuum is achieved but about 5 to 10 minutes later it starts
} dropping. The problem developed over time. In the beginning, it would drop by very little and
} go back up again. Later on, it started dropping by a lot more and it took longer to get back
} up again. Now, the vacuum is almost competely lost. Does anybody know what might be causing
} this problem? Any tips or suggestions? We appreciate the help and thank you all in advance.
}
}
}



From daemon Thu Oct 18 19:18:17 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 18 Oct 2001 20:14:35 -0400
Subject: Re: art and science

Contents Retrieved from Microscopy Listserver Archives
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Shaf,
My wife will tell you art can be taught just like science. There are
basic principles involved that anyone can learn. Just like science,
though, some have more "innate", "natural",( you choose the term)
ability. This is why there are some outstanding artists and some
outstanding scientists. The creativity is the difference between the
norm and the great in both cases. The same holds in most every field of
endeavor that I can think of.

Ken Converse


michael shaffer wrote:

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} -----------------------------------------------------------------------.
}
}
} Judi Bowden writes ...
}
} } ...
} } Do you see a link between science and art? If so, how and why?
} } ...
}
}
} I think you are mixing apples and oranges. "Science" is a process by
} which we learn ... "art" is something we create and/or observe. However,
} that is not to say we cannot use a scientific process to learn how to
} create, or create differently.
}
} my 0.02 ... shAf :o)
}
}
}
}



From daemon Thu Oct 18 19:25:51 2001



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 19 Oct 2001 10:26:29 +1000
Subject: Re: TEM of cadaver tissue

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Hi Allan,

I've looked at numerous post mortem retina specimens. I agree with
Sara, though do find that glut fixation is better than form, even
though there are P/M effects to consider. Unfortunately it's not
always possible to say that a tissue from, say, 8 hours P/M will be
better than one from, say 12 hours. Other variables, such as
temperature of the body during that time come into play. Sometimes 8
(or 12 or even 15) hours P/M is fine, sometimes useless. Obviously
however, less is generally better. Some cells seem a lot more
"fragile" than others and deteriorate very rapidly - it's useful to
find, for your tissue, which these are and use them as a rough
measure of P/M effects. Blood vessels do seem pretty tough and I've
seen quite good vessels when surrounding tissue is a mess. I haven't
had to embed much for EM after long term storage so can't answer
question 3, but have done immuno work on form fixed material up to
years old and it was fine for LM. As for what U/S changes to expect
in P/M material, that's a question that could take up a whole book!
Just look carefully and compare your own specimens with each other
and with what you know to be good U/S. It's all fairly obvious with
experience.

Diana

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318


From daemon Thu Oct 18 22:06:31 2001



From: integer-at-www.god-emil.dk
Date: Fri, 19 Oct 2001 04:58:01 +0200 (CEST)
Subject: Re: art and science

Contents Retrieved from Microscopy Listserver Archives
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} Some examples are: a screen made of 6000 Petri dishes;

lekker [delicious]


} micro art
viewed with a light microscope and made with drosophila wings,

micro.theatre + ballet



http://www.m9ndfukc.org/data/filmz/242.koelenterate.mov

http://www.eusocial.com/nato.0+55+3d/gm/242.055.koelenterate.sit.bin



scienza sine ars nihil est
ars sine scienza nihil est




/_/
/
\ \/ i should like to be a human plant
\/ __
__/
i will shed leaves in the shade
\_\ because i like stepping on bugs



*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--*--
Netochka Nezvanova nezvanova-at-eusocial.com
http://www.eusocial.com

http://www.ggttctttat.com/!
n r . 5 !!! http://steim.nl/leaves/petalz
*--*--*--*--*--*--*--*--*--*--*--*--*-- --*--*--*--*--*--*--








From daemon Fri Oct 19 01:50:35 2001



From: Visitec-at-t-online.de (Martin Klein)
Date: Fri, 19 Oct 2001 08:40:51 +0200
Subject: AW: semming a capacitor

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Hi Mark,

Yes it is possible to check electronic devices. From our experience it may
give better results if you are applying a load (mechanical and/or thermal)
to the device or much better to the entire pcb. Sometimes these tiny cracks
are occurring only if the load is applied.

Happy semming
Martin

VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de
* Home of world's largest SEM *


-----Ursprüngliche Nachricht-----
Von: Mark Riggs [mailto:Mark.Riggs-at-asml.com]
Gesendet: Donnerstag, 18. Oktober 2001 22:11
An: microscopy-at-sparc5.microscopy.com
Betreff: semming a capacitor


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


just heard a phone message inquiry about the ability of a sem to evaluate
"micro-cracking" of a capacitor's outer shell. the requestor believes the
"micro-cracking" might be indicative/source of performance problems. is
this possible, and how do i sem it? mr




From daemon Fri Oct 19 02:36:34 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Fri, 19 Oct 2001 02:32:51 -0500
Subject: Re: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
}
} "Do you see a link between science and art? If so, how and why?"
}
} Judi,
}
} Linking creativity in art and science:
}
} Creativity in art is not substantially different from creativity in
} science. Creativity in art/science is not a person suddenly struck by a
} blinding flash of inspiration/insight and then producing a masterpiece.
} Even the most radical artists/scientists start from classical knowledge
and
} then spend years polishing their technique. [Picasso, Mozart, Einstein]
} (source: Julio Ottino, lecturer on "Creativity ? an Art View", an
} accomplished artist and Professor, and Chair, Department of Chemical
} Engineering, Northwestern University, Evanston, Illinois, USA).
}
} "Even the most talented persons do not reach world class performance in
} their fields until they have devoted about 10 years or more of rather
} single minded attention to becoming an expert". What chiefly distinguishes
} creative thinking from more mundane forms [in art/science] are Ambiguity,
} Persistence and Knowledge - (i) willingness to accept vaguely defined
} problem statements and gradually structuring them, (ii) continuing
} preoccupation with problems over considerable periods of time, and (iii)
} extensive background knowledge in relevant and potentially relevant areas.
} (source: H. Simon, Nat'l Acad Sci Proc, 80, 4569-4571, 1983).
}
} Art is the selection from a great number of variables (source: book, Zen
} and the Art of Motorcycle Maintenance). There are also a great number of
} variables to select from at the start of a new science project.
}
}
Creativity is not bounded by art or science. The closer you get to the
leading edge the more science becomes an art. The only difference is in art
you have critics and in science you face peer review.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger




From daemon Fri Oct 19 07:00:33 2001



From: jshields-at-cb.uga.edu
Date: Fri, 19 Oct 2001 07:52:31 -0400
Subject: Re: Dirty Nematodes

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Hi Dan,
We used to remove the stylet secretions from rot-knot and
soybean cyst juveniles (very sticky stuff) by adding 1 part of a high
pH buffer (0.1 M Tris-NaOH, somewhere around pH 11-12) for 2
minutes or so.
Of course, we were attempting to collect the secretions and not
preserve the nematode. Although they seemed to be fine, as we
could usually do this for several days after washing the 'tode.
John Shields
EM Lab
University of Georgia


On 18 Oct 2001, at 14:54, Dan Bumbarger wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} I am trying to do some SEM on nematodes with elaborate structures on
} their front ends. Unfortunately, these critters secrete some sort of
} sticky residue around these structures that makes it difficult to get
} good, clean specimens. The "stuff"is likely some sort of glycoprotein
} goo and I should (hopefully) be able to rinse it off with something,
} without damaging the integrity of the collagenous cuticle and without
} giving me other sorts of artifacts. Any ideas? --
} ______________________________________________ Dan Bumbarger Graduate
} Student Depts. of Biology and Nematology University of California,
} Riverside Riverside, CA 92521
} ______________________________________________
}
}
} "With a little more deliberation in choice of pursuits, all men would
} perhaps become essentially students and observers, for certainly their
} nature and destiny are interesting to all alike. In accumulating
} property for ourselves or our posterity, in
} founding a family or a state, or acquiring fame even, we are
} mortal; but in dealing with truth we are immortal, and need fear no
} change nor accident."
}
}
} -H.D. Thoreau, "Walden"
}




From daemon Fri Oct 19 08:09:46 2001



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 19 Oct 2001 14:00:13 +0000
Subject: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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Well, yes and no. I agree that creativity is basal to both art and
science, but surely the two are fundamentally distinct at least as
far as their aims are concerned. The aim of science is to discover
the truth (whatever that may be! - see e.g. Popper) about the
natural world and its properties. Scientists use their creative
imagination to devise ways of discovering facts about the world.
Artists use their creative imagination to create objects -artefacts-
which never have been of this world before. Scientists also create
artefacts, but usually blush when one is pointed out to them. I find
it fascinating that the most imaginative scientists are also
profoundly aware of the arts, and are frequently very accomplished
artists. Equally many artists are intrigued by the findings of
science, if not actually by the methods, so the possibility exists of
considerable common ground between the two activities as far as
the mental / intellectual processes are concerned. But the key
features that distinguish science from art are the accumulation and
evaluation of information, the use quantitative analysis and of logic,
and the application of a rigorous scientific methodology to
distinguish fact from non-fact. None of these things are
fundamental to art, indeed with a few exceptions their use seems
to be at best optional, if not scorned altogether. In other words it is
the prerogative of the artist to let the mind be totally free of the
contraints of reality. Scientists do not have that luxury.

Chris


} Creativity is not bounded by art or science. The closer you get to the
} leading edge the more science becomes an art. The only difference is in art
} you have critics and in science you face peer review.
}
} Gordon
}
} Gordon Couger
} Stillwater, OK
} www.couger.com/gcouger
}
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================


From daemon Fri Oct 19 10:12:48 2001



From: William Oxberry :      William_Oxberry-at-downstate.edu
Date: 10/17/2001 3:30 PM
Subject: High vacuum on Hitachi H600

Contents Retrieved from Microscopy Listserver Archives
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when was the last time your DP oil was replaced? It could be 'cracked' or,
as was explained to me by a JEOL engineer, saturated by trying to remove
more air from the column than it was capable due to a variety of reasons
like too short a prepump(roughing) time, and/or improper valving sequences
from rough pumping to high vac or a small column leak.


____________________Reply Separator____________________




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Hi everyone,

We are having a problem with the high vacuum on our HItachi
H600 TEM. When the microscope is
first turned on, the high vacuum is achieved but about 5 to 10
minutes later it starts
dropping. The problem developed over time. In the beginning,
it would drop by very little and
go back up again. Later on, it started dropping by a lot more
and it took longer to get back
up again. Now, the vacuum is almost competely lost. Does
anybody know what might be causing
this problem? Any tips or suggestions? We appreciate the help
and thank you all in advance.




From daemon Fri Oct 19 11:50:11 2001



From: Nicol Aitken :      nicol-at-semiconductor.com
Date: Fri, 19 Oct 2001 12:39:35 -0400
Subject: off topic-RIE

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Message-ID: {B083A496531ED5119B6F00010272E3B81B091E-at-xserver-200.semiconductor.com}


Hello semmers,
Since I have had such great help in the past from all of you, I have
decided to once again seek information from this great source. I am
wondering if any of you use reactive ion etching, or even more helpful would
ICP-RIE. If so, it would be great if you could answer some questions for me.
Please contact me off line, as it isn't really a microscopy question.
Thanks again
Nick



Nicol Aitken
Sample Preparation and Imaging Specialist
Research and Development
Semiconductor Insights Inc.
email:nicol-at-semiconductor.com
(613)599-6500 ext.4300



From daemon Fri Oct 19 14:42:31 2001



From: Nora Pratta- Silvia Montoro :      csedax-at-ceride.gov.ar
Date: Fri, 19 Oct 2001 16:30:26 -0300
Subject: SEM: Paper fibers sample preparation

Contents Retrieved from Microscopy Listserver Archives
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Hi all members,

a week ago I wrote asking about a problem
related to image distortion on our SEM. Thanks to all of you who wrote
suggestions to us. the technicians are studying the solution of the problem.


My question now is related to sample preparation of paper for studying the
thickness of the fibers. The idea is to obtain cross sections of paper
sheets showing the sections of the fibers. How would you prepare it in order
to avoid swelling or deformation of the sections? Embedding would not be the
ideal method since the surface of the same paper has to be observed.

Any suggestions will be very much appreciated.

Thanks a lot

Silvia Montoro
CERIDE
G|emes 3450
3000 Santa Fe - Argentina
csedax-at-ceride.gov.ar



From daemon Fri Oct 19 14:42:31 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Fri, 19 Oct 2001 14:32:57 -0500
Subject: Re: semming a capacitor

Contents Retrieved from Microscopy Listserver Archives
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Mark: a lot depends on what type of capacitor it is. More detail about the cap would be helpful. In my experience, small ceramic surface-mount caps are more prone to problems in the area than large metal-can electrolytics. I agree with Martin Klein that
examining the cap in its native habitat would be the best, but it all depends on how big your SEM chamber is and where you suspect the cracks might be occurring. If they are between the cap and the board; they'll be really hard to see in the SEM. A word of
advice: this is best done in a field-emission SEM or variable-pressure SEM because charging is going to be a big problem and I'm assuming you don't want to coat the board with gold or carbon.

Mark Riggs wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} just heard a phone message inquiry about the ability of a sem to evaluate "micro-cracking" of a capacitor's outer shell. the requestor believes the "micro-cracking" might be indicative/source of performance problems. is this possible, and how do i sem it? mr

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
SC Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Fri Oct 19 15:28:00 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Fri, 19 Oct 2001 15:20:47 -0500
Subject: Schematics for boards Polaron evaporator

Contents Retrieved from Microscopy Listserver Archives
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Listers,

We have a Polaron Turbo Coater carbon evaporator, model E6700, probably 10 -
15 years old, which is experiencing some problems. In spite of acceptable
vacuum and functioning pirani gauges, the penning gauge is not turning on,
and one of the fuses is blowing every time we turn it on.

We don't have schematics for any of the boards in the instrument, just a
schematic which gives an overview of the entire instrument, but no specifics
of individual boards.

If anyone out there has schematics for any boards in this instrument, and
would be willing to share this info, could you please contact me off line?

Thanks,
Wharton Sinkler
UOP LLC
25 E. Algonquin Rd.
Des Plaines, IL 60017-5017




From daemon Fri Oct 19 16:07:15 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 19 Oct 2001 14:00:07 -0700 (PDT)
Subject: references

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Hello,
I'm trying to find a couple of recommended references for phytoplankton
and other small items to be fixed/dried for SEM. The samples have the
problem of getting lost during the CPD process. I have the following,
but I cannot find the book Scanning Electron Microscopy II. Has anyone
seen these references and are they correct as I have them:?

Preparation of Microbiological Specimens for Scanning Electron Microscopy,
L.P. Watson, A.E. Mckee, and B.R. Merrell. Scanning Electron
Microscopy/1980/II, pages 45-56.

Specimen Preparation Techniques for Aquatic Organisms, T.K. Maugel, D.B.
Bonar, W.J. Creegan and E.B. Small. Scanning Electron Microscopy/1980/II,
pages 57-77.










\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Fri Oct 19 19:40:46 2001



From: Peter Donahoe :      glencadia-at-berk.com
Date: Fri, 19 Oct 2001 20:34:57 -0400
Subject: RE: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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Dear Judy,
To your question: Do you see a link between science and art? If so, how and
why?
The mediating event between the interiority and exteriority of our
experience of the world is in the aesthetic moment according to Jamesian
philosophy. It forges the union between past and future, between certitude
and expectation. As many other have said previously in this
discussion -especially David Barnard's excellent observation - their work
often hinges on the awareness of beauty within the work. It ties together
the craft of rigorous method with optimism of our theory.

I have spent thirty years working in photography in all its aspects, from
street documentary to fashion studio work, from mug shots to gallery work.
One of the most exciting bodies of work that I ever produced was as student
of EM at the VA Center in Albany, NY, now sadly closed. I shot something
like 4 times the amount of film than most students did. Why? Because the
electron microscope, especially the TEM, is nothing more than a camera, but
what a wonderful camera it is! It investigates a landscape, searches for a
recognizable form, establishes proportions and makes measurements, always
looking for 'significance' however that may be defined . In short, exactly
the same thing I try to do in conventional landscape photography. It' s all
a part of the attempt to make sense of the perceptible world, or to make the
imperceptible visible and then intelligible.

I recommend to you the following:
Art Forms in Nature, Ernst Haeckel and Karl Blossfeldt's Art Forms in the
Plant World

Good Luck,

Peter Donahoe




From daemon Fri Oct 19 20:05:03 2001



From: Damian Neuberger :      neuberger1234-at-home.com
Date: Fri, 19 Oct 2001 20:03:28 -0500
Subject: Re: Ask-A-Microscopist:LM color changes in photographs

Contents Retrieved from Microscopy Listserver Archives
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Fred and Michelle,
I'd like to add a few additional and perhaps unrelated points to getting
consistent results. Having done a a lot! of photomicrography especially for
publication in text books, another method for getting consistent results is
to buy a block of film that is all from the same lot (check the labels). I
also learned that film is made to have a known storage or shelf time until
it reaches the optimum color balance, so you have to find out what that is
as well. Finally, I had to find a processor that ran test strips at the
beginning of the day to assure that the process was correct, then I made
sure that they did my film first thing in the morning. Regarding filters, I
had to buy a set of light balancing and color compensating (CC) filters to
get "perfect" color balance. Naturally, I always bracketed by about 1 stop
in 1/3 stop increments as my time was more expensive than the film. I
learned that the voltage fluctuated slightly (dropped) and had to turn the
lamp on and wait about 30 min to get it stable. Obviously I was doing very
critical color work and this all may be overkill for you.
Regards from an oldtimer whose using digital now :-)
Damian
}
} Dear Michelle,
} Had the same problem when I was starting out. It turned out that I
} was alternating with someone who was using tungsten film and removed the
} daylight filter for his photography. On another occasion when I was the
} lone user, I found that there were lots of voltage changes during the day
} and photos taken at one time were different from those taken at another.
} The immediate way to handle the voltage problem is to check the power
supply
} voltage level for every exposure.
} When I had a class taking photos, I used a tabular sign-in sheet
} with columns for: Power supply setting; Iris diaphragm open; field
} diaphragm adjusted; objective; time of photo; trinocular prism setting
(1,2,
} or 3); etc. I think you get the point. If your line voltage is at fault,
} the only way you can prove it is to have someone perform a time study to
} record those changes over a 24hr period at your outlet.
} The next problem that I have seen is caused by picking a roll of
} film on Monday that is designated for use with a tungsten source and
another
} roll on Thursday that is designated for use with daylight.
} Oh, yes! AND FINALLY, if your photo detector is set for spot
} metering rather than averaging, a small change in location can/will have a
} great effect on the result, which will appear to be unpredictable.
} From all of the above, your best bet is the proper compensating
} filter when using artificial light (LBD (Olympus) for daylight-type(5500K)
} and LBT for tungsten-type(3400K) films)
} For years I have treasured a book I acquired from Olympus entitled,
} "How To Improve Photography Through the Microscope" (Part # M132E-0886T).
} There is another that I acquired from Leitz before it became Leica.
}
} Hope that one small part of this helps,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}
} } ----------
} } From: mryder-at-brookes.ac.uk
} } Sent: Wednesday, October 17, 2001 8:00 AM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Ask-A-Microscopist:LM color changes in photographs
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (mryder-at-brookes.ac.uk) from
} } http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} } October 17, 2001 at 05:31:30
}
} --------------------------------------------------------------------------
} } -
} }
} } Email: mryder-at-brookes.ac.uk
} } Name: Michelle Ryder
} }
} } Organization: Oxford Brookes University
} }
} } Education: Graduate College
} }
} } Location: Headington Campus, Gipsy Lane, Oxford, OX3 0BP
} }
} } Question: Why do my wholemount lowish power root
} } photographs sometimes come out yellow, on the
} } same film, with the same lighting. Sometimes
} } the same root taken a few minutes later will
} } be yellow when it was the usual white on a previous frame.
} }
} }
} }
}
} --------------------------------------------------------------------------
} } -
} }
} }
}
}



From daemon Fri Oct 19 20:18:42 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 19 Oct 2001 21:12:54 -0500
Subject: Preparation of paper cross-sections

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Silvia Montoro wrote:
=========================================================
............... My question now is related to sample preparation of paper
for studying the thickness of the fibers. The idea is to obtain cross
sections of paper sheets showing the sections of the fibers. How would you
prepare it in order to avoid swelling or deformation of the sections?
Embedding would not be the ideal method since the surface of the same paper
has to be observed.

Any suggestions will be very much appreciated.
==========================================================
You are quite correct to be concerned about possible swelling and/or
deformation of the paper structure if embedded. However, another concern is
the possible swelling of the fibers themselves with the embedding resin,
thereby giving you a diameter figure that is much larger than reality.

Our approach has been to gold coat both sides in a sputter coater before
embedding. It is always a trial and error exercise to determine the "right"
thickness of the gold layer, the purpose for which is to a) passivate
individual fibers to reduce the tendency of the embedding resin to penetrate
and swell individual fibers and b) reduce the "elasticity", or the ability
of the larger structure to be changed by the embedding media. We find that
Pt is somewhat better than gold because of its smaller grain size and the
greater hardness results in a lowered tendency for the metal to "smear".

We have always had our best results with our own SPI-Pon™ 812 Epoxy
Embedding Resin Kit but we believe some of the other Epon® "substitutes"
would work just as well.

Using room temperature sectioning, you can then thin section the block and
by TEM, the gold layer acts as a nice decoration line outlining the fibers.
If there has been excessive fiber swelling, usually there are signs that
this is happening, at least once one develops experience in looking at such
micrographs. If you don't have a TEM, you can look by SEM at the "faced-off
-piece", that is, the block from which the last section was taken. So long
as you are using a thin layer of conductive metal, usually there is enough
contrast from the gold decoration line to resolve well the individual fibers
. Obviously this technique will work better with thinner than it will with
thicker papers. It might not be the perfect solution but it does permit one
to make measurements on the fiber diameters. Also, at times a short plasma
etching with oxygen on the faced-off-surface will enhance further the
contrast between the gold layer and the fibers and embedding resin.

If we would be doing this kind of work today, and wanting to look at the
faced-off-piece by SEM, instead of coating with gold we would coat with 1 nm
of osmium metal in the OPC Osmium Coater. Although a heavy metal, that
thickness is thin enough that it does not interfere with being able to see
contrast from the underlying gold (decoration) layer.

Note: You should be using a "materials science" rather than a life science
(LS) diamond knife since they are cheaper than an LS knife and also, the
inorganics in most papers would "kill" a LS knife for future use on LS
samples.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================








From daemon Fri Oct 19 21:45:27 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 19 Oct 2001 22:35:01 +0800
Subject: Re: science and art

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on 10/18/01 11:32 PM, Robert Underwood at underwoo-at-u.washington.edu wrote:

}
} I heard a definition of art when I was in photography school many years
} ago. I wish I knew the source...
}
} Art is anything, that when abstracted from its surroundings, embibes its
} viewer with a hieghtened or altered sense of thier own existence.
}
Dear Robert and other listers,
When I was an undergraduate, I was arrogant enough to propose a
definition of art--one which would include such pieces as a number of bricks
arranged in a neat pile (on display at MOMA). I defined art as the
projection of the universe onto some chosen set of limitations. Good art
enlightens the artist and audience about the nature of the universe by
reducing it to an understandable piece; whereas bad art is unsuccessful in
this enlightenment (so the value judgement rests with the audience).
Not only do I still think that this is a valid definition, but I can
also see how this applies very obviously to science. IMHO, therefore,
science and art are not separate, but science is art.
Yours,
Bill Tivol



From daemon Fri Oct 19 22:25:38 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 19 Oct 2001 20:24:24 -0700
Subject: Re: off topic-RIE

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I'm not sure that this is off topic. Of course, Nestor can correct me
if I am wrong.

I do RIE and ICP-RIE etching of IC passivation followed
by SEM imaging on a somewhat routine basis. ICP is
a new method and is quite improved over traditional RIE
methods. Passivation removal is quite difficult based on
power, pressure and gasses. For oxide, I use CF4 and
O2 at a pressure of about 10mT. I'm not certain of the power.

My particular work in this area is to SEM image ICs which
have been de-passivated. RIE and ICP-RIE accomplish this
in different final outcomes. I find dramatic differences between
packaged die and bare die.

gary g.


At 09:39 AM 10/19/2001, you wrote:

} Hello semmers,
} Since I have had such great help in the past from all of you, I have
} decided to once again seek information from this great source. I am
} wondering if any of you use reactive ion etching, or even more helpful would
} ICP-RIE. If so, it would be great if you could answer some questions for me.
} Please contact me off line, as it isn't really a microscopy question.
} Thanks again
} Nick
}
}
}
} Nicol Aitken
} Sample Preparation and Imaging Specialist
} Research and Development
} Semiconductor Insights Inc.
} email:nicol-at-semiconductor.com
} (613)599-6500 ext.4300



From daemon Sat Oct 20 15:10:59 2001



From: Hong Yi :      hyi-at-emory.edu
Date: Sat, 20 Oct 2001 15:53:56 -0400 (EDT)
Subject: Antibody search again

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Dear All:
Thank you for those who replied to my posting regarding
anti-amylase antibody search. I eventually located one at the Chemicon.
Now, I am looking for a polyclonal anti-synaptophysin antibody. I
know that there are many companies carry this product. But I would like
to know users' experiences before I decide on which one to purchase from.
If you have used this antibody from a particular company, would you share
the following information with me. Thank you a million in advance.

Supplier name:
Samples labeled: (cell culture or tissue type, species...)
Labeling type: (immuno fluorescence, immunoperoxidase, or immunogold?)
Labeling procedure: (pre-embedding or post-embedding?)
Fixative used:
Embedding method used:

By the way, for those who do not have Linscott's directory, Try
www.sciquest.com. I do my antibody search on this site. It is very
easy to use.

Hong




From daemon Sat Oct 20 16:42:08 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Sat, 20 Oct 2001 17:41:07 -0400
Subject: Re: Semming a capacitor - Ceramics

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Mark,

The type of capacitor determines what you have to do. Titanate type
chip capacitors consist of alternating metal electrode sheets sandwiched
between the dielectric layers, both of which are laid down by printing
methods and then fired into a monolithic structure. End metallization
is applied so that the alternate protruding plate layers are
electrically joined to a metal coating on each end. It is a robust
structure but because it is made of very hard, rigid material, locating
an internal fracture can be difficult. A fracture that extends to the
outside can usually be seen in the SEM without coating the specimen.
The tendency for dielectrics like titanates to charge is highly
dependent on the orientation of the subject to the beam. One can
usually tilt the faces of the capacitor to find an angle where charging
is not a problem and then simply search for the crack. Using the
backscatter detector instead of the secondary electron detector will
also help to minimize charging interference.

Apart from the SEM, a fluorescent dye can also be used for an exterior
crack. Magniflux makes such compositions but you can find others by
doing a search with the terms "dye penetrant" and "non-destructive
testing". The usual procedure is to place the cap in the dye inside a
small vessel in which the pressure can be reduced. The vessel is
partially evacuated so that upon return to room pressure the dye is
drawn into the crack. The cap is then quickly rinsed and observed under
a ultraviolet light with a low power microscope. Dye tends to creep out
of the crack revealing its position.

Internal cracks can take different forms. One problem type is where the
end metallization separates from the edge of one or more of the plates.
This is a common source of temperature dependent, intermittent faults.
In this case some destructive sample prep is required and there is a
chance that the defect will be lost or "healed" in the course of the
prep. One can determine the edge direction of the plates and then,
using metallographic methods, grind and polish off the exterior ceramic
coating to expose the edges of the plates. Don't go beyond the minimum
necessary to expose the edges. Use a nap-less cloth for polishing, very
light pressure and diamond abrasive to minimize smearing of the
metallization. The point where each plate intersects it's respective
end cap is a critical area to check for discontinuities. In the SEM you
can now use the secondary detector and some charging to your advantage
by grounding one endcap and leaving the other electrically floating. A
plate that is charging up while its companions are not reveals where a
discontinuity exists. Conversely, a plate that fails to charge when its
companions on the same endcap are doing so reveals a short to the other
endcap. Simple cracks in the internal dielectric can also be revealed
by orienting the subject to where charge accumulates and edge effects
accentuate the charging. If your SEM is set up for specimen current
imaging, compare what you see in this mode with the results of SEI and BEI.

Good luck.

John Twilley
Conservation Scientist


Mark Riggs wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com {mailto:ListServer-at-MSA.Microscopy.Com}
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} -----------------------------------------------------------------------.
}
} just heard a phone message inquiry about the ability of a sem to evaluate "micro-cracking" of a capacitor's outer shell. the requestor believes the "micro-cracking" might be indicative/source of performance problems. is this possible, and how do i sem it? mr



From daemon Sun Oct 21 21:15:30 2001



From: dhicks3345-at-kilten.com :      dhicks3345-at-kilten.com
Date: Sat, 29 Sep 2001 23:11:56 +0800
Subject: All foreign residents. Check out this offer

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From daemon Mon Oct 22 08:16:10 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 22 Oct 2001 09:05:06 -0400
Subject: RE: Dirty Nematodes

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Morning Dan,
One might suggest warm physiological saline, but some will recommend
you try what one of my children called 'goozyme', three others called
'spit', or I call, saliva, but most will recommend amylase, or some other
-ase, which will cost unless you find some in a nearby refrigerator. While
there are some who will interrupt this conversation by mentioning that the
former -ase (that is, amyl-), only hydrolyzes glocogen, I have seen it do
nicely on what used to be called mucous secretions, if your source of
amylase is saliva (p.c.?). NOTE1: All protein-goo is preserved
(cross-linked) by fixation longer than necessary to terminate (p.c.?) the
subject (p.c.?). If it must be removed, then one should try to do it prior
to fixation - not necessarily prior to termination (p.c.?). NOTE2: The
above methods can be tried in any sequence for good or ill, but be
forewarned that your actions will be misunderstood if you are seen by
colleagues to be expectorating (p.c.?) at a worm. NOTE3: Of course, if you
continue to work with worms with rhinitis, you must suffer the consequences.
In all seriousness, however, if one's worm has a cold, could one preempt the
goo with oxymetazoline hydrochloride.
This problem, of course, will remind us all that one cannot grasp a
live hagfish [old or young!], because it ties itself in a knot, secretes a
bucket of goo, and then extricates itself by moving the knot (a posteriori),
all of which hasn't much to do with your goo Dan, but does present a much
more messy problem that others [I know not who!] have had, which should make
you feel better about your, only slightly, goo'd animal.
It is, after all, Monday morning, and having seen our grandson off
on Saturday, Grandma and Grumpy spent the rest of the weekend,
unsuccessfully trying to catch up on sleep. As always, we hope that if the
above doesn't bring immediate ease of the problem, it will, at least
engender a smile.

Regards and commiseration to all those who have wormy problems,

Fred Monson

(p.c.) = (polite conversation?), of course!

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
So, I will answer the phone with my name, not the name of my workplace.
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Dan Bumbarger
} Sent: Thursday, October 18, 2001 5:54 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Dirty Nematodes
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am trying to do some SEM on nematodes with elaborate structures on
} their front ends. Unfortunately, these critters secrete some sort of
} sticky residue around these structures that makes it difficult to get
} good, clean specimens. The "stuff"is likely some sort of glycoprotein
} goo and I should (hopefully) be able to rinse it off with something,
} without damaging the integrity of the collagenous cuticle and without
} giving me other sorts of artifacts. Any ideas?
} --
} ______________________________________________
} Dan Bumbarger
} Graduate Student
} Depts. of Biology and Nematology
} University of California, Riverside
} Riverside, CA 92521
} ______________________________________________
}
}
} "With a little more deliberation in choice of pursuits, all men would
} perhaps become essentially students and observers, for certainly
} their nature and destiny are interesting to all alike. In
} accumulating property for ourselves or our posterity, in
} founding a family or a state, or acquiring fame even, we are
} mortal; but in dealing with truth we are immortal, and need fear no
} change nor accident."
}
}
} -H.D. Thoreau, "Walden"
}
}
}


From daemon Mon Oct 22 11:14:39 2001



From: Tom Tottleben :      tomtot-at-charter.net
Date: Mon, 22 Oct 2001 10:59:40 -0500
Subject: LM: The Coolpix - Nikon Optiphot Connection

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To: Great Minds in the Halls of Science Wisdom!

I realize this topic has been hammered out in this forum a few times
(for everything except the Nikon Optiphot superwide
trinocular) ......can someone direct me to any information that would be
helpful? ("I can do this .......yeah!!!") Thanks,

Tom Tottleben
Tottleben Scientific Co
PO Box 24
104 Burns Farm-W.Ct.
Edwardsville, IL. 62025
618 656 9008 Office
618 656 9599 Fax
618 558 9008 Cell
800 283 9997 Orders
tomtot-at-charter.net
www.tscmicroscopes.com



From daemon Mon Oct 22 13:16:27 2001



From: Nami Choe :      c56nc-at-morgan.ucs.mun.ca
Date: Mon, 22 Oct 2001 15:37:43 -0230 (NDT)
Subject: microdissection before SEM/TEM

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Dear microscopy members;

Hello. I am a marine biology graduate student at Memorial University of
Newfoundland, Canada. One of my research topic is to study the
microstructure of the statolith in the pelagic marine tunicate (Larvacea),
Oikopleura vanhoeffeni. The statolith is a small cylindrical calcium
build up (10 micrometer diameter, 20 micrometer height), attached to the
brain of the tunicate. This bony structure is contained in the
membraneous statocyst and probably works as a gravity detector.
I would like to isolate this structure for SEM and TEM analyses. After
this, Xray microcroprobe and other procedures will follow.

Dissecting the brain is easy but taking out the statolith from the
statocyst is the difficult part. Visualizing the statolith can be done by
calcium specific stain, alizarin red. I have tried to dissect the
statolith out by using fine insect pins ( { 20 micron tip size), but the
pins are too large. Doing this under dissecting scope is impossible since
4x magnification does not allow me to do such microdissection.

I tried to dissolve the statocyst with bleach and hydrogen peroxide to
isolate inorganic statolith. However, after this procedure, I can no
longer visualize the statolith since alizarin red stain dissolves
instantaneously in these chemicals.

Sonication was also done to rupture the statocyst and pop the statolith
out but it did not work.

Do you have any suggestion on how I can separate this structure?
Sincerely,
Nami Choe
____________________________________________________________________________

Nami Choe
Ocean Science Centre
Memorial University of Newfoundland
St. John's, NF
A1C 5S7 Canada
phone:(709)737-3247
fax:(709)737-3220





From daemon Mon Oct 22 16:07:14 2001



From: Wayne Reitz :      Wayne.Reitz-at-ndsu.nodak.edu
Date: Mon, 22 Oct 2001 19:39:48 -0500
Subject: older Ziess optics

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I don't know anything about the structure you describe, but check out
these references:

Howell DN, Miller SE. Identification of viral infection by confocal
microscopy. 1999. Methods in Enzymology. Academic Press, 307:573-91.

Miller SE, Levenson RM, Aldridge C, Hester S, Kenan DJ, Howell DN.
1997. Identification of focal viral infections by confocal microscopy
for subsequent ultrastructural analysis. Ultrastruc Pathol 21:183-193.


Briefly, we make "vibratome" sections, stain the slices in propidium
iodide, and examine the tissue by confocal microscopy for areas of
interest. We also sometimes use antibodies with a fluorescent tag. We
then cut out a smaller area including the structure of interest (1 x 1
mm) in a shape of the state of Nevada so that you will know if the
section gets flipped over.

If your structure is something that you can recognize morphologically, and
you can deal with a 100 to 400 um thick section, this might work.

Good luck,

Sara Miller


On Mon, 22 Oct 2001, Nami Choe wrote:

} Date: Mon, 22 Oct 2001 15:37:43 -0230 (NDT)
} From: Nami Choe {c56nc-at-morgan.ucs.mun.ca}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: microdissection before SEM/TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear microscopy members;
}
} Hello. I am a marine biology graduate student at Memorial University of
} Newfoundland, Canada. One of my research topic is to study the
} microstructure of the statolith in the pelagic marine tunicate (Larvacea),
} Oikopleura vanhoeffeni. The statolith is a small cylindrical calcium
} build up (10 micrometer diameter, 20 micrometer height), attached to the
} brain of the tunicate. This bony structure is contained in the
} membraneous statocyst and probably works as a gravity detector.
} I would like to isolate this structure for SEM and TEM analyses. After
} this, Xray microcroprobe and other procedures will follow.
}
} Dissecting the brain is easy but taking out the statolith from the
} statocyst is the difficult part. Visualizing the statolith can be done by
} calcium specific stain, alizarin red. I have tried to dissect the
} statolith out by using fine insect pins ( { 20 micron tip size), but the
} pins are too large. Doing this under dissecting scope is impossible since
} 4x magnification does not allow me to do such microdissection.
}
} I tried to dissolve the statocyst with bleach and hydrogen peroxide to
} isolate inorganic statolith. However, after this procedure, I can no
} longer visualize the statolith since alizarin red stain dissolves
} instantaneously in these chemicals.
}
} Sonication was also done to rupture the statocyst and pop the statolith
} out but it did not work.
}
} Do you have any suggestion on how I can separate this structure?
} Sincerely,
} Nami Choe
} ____________________________________________________________________________
}
} Nami Choe
} Ocean Science Centre
} Memorial University of Newfoundland
} St. John's, NF
} A1C 5S7 Canada
} phone:(709)737-3247
} fax:(709)737-3220
}
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From root Mon Oct 22 17:12:25 2001
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Reply-To: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}


Nami
My guess is you are probably already on the right tack with your
attempts to isolate the inorganic statoliths by treatment with
hypochlorite and peroxide. If you next wash these reagents out of
your preparation, Alizarin red will again stain the statoliths, so you
can see them. The main problem may be to handle the statoliths during
these treatments. One effective way of containing such small objects
through a series of reactions or fixation/dehydration steps is to
contain them in a Millepore filter unit, exchanging the solutions
using a disposable syringe. There is a wide range of filter media
with different chemical compositions and pore sizes to choose from.
The majority will retain object in the size range of your statoliths.

hope this helps
Chris Jeffree

----- Original Message -----
} From: "Nami Choe" {c56nc-at-morgan.ucs.mun.ca}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, October 22, 2001 7:07 PM


to all,
My question concerns connecting a light source to a metallograph.
I have inherited a Zeiss metallograph with a mismatched light source,
Optiquip. The unit arrived without a collar connecting the lamp to the
metallograph. The equipment is consists of
Zeiss metallograph (gray), 47 17 65
Optiquip light source (creme), model 770.
I would appreciate comments from anyone having experience connecting these 2
components or knows of a supplier that can assist. Apparently, the length
of the collar is critical and perhaps the diameter.

Wayne Reitz
North Dakota State University


From daemon Tue Oct 23 07:40:48 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 23 Oct 2001 08:28:40 -0400
Subject: RE: The Coolpix - Nikon Optiphot Connection

Contents Retrieved from Microscopy Listserver Archives
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We have mounting solutions for the Coolpix cameras
to fit most microscopes through a C-mount, phototube or
eyepiece tube. We have the cameras and accessories as well.
A number of configurations are available depending on the
microscope.
I would be happy to assist in finding the proper
mounting, for most instruments we need brand and model
number to determine requirements.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com


To: Great Minds in the Halls of Science Wisdom!

I realize this topic has been hammered out in this forum a few times
(for everything except the Nikon Optiphot superwide
trinocular) ......can someone direct me to any information that would be
helpful? ("I can do this .......yeah!!!") Thanks,

Tom Tottleben



From daemon Tue Oct 23 07:40:48 2001



From: charlted-at-mcmaster.ca ()
Date: Tue, 23 Oct 2001 07:32:28 -0500
Subject: Ask-A-Microscopist: embedding techniques for light microscopy

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (charlted-at-mcmaster.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
October 22, 2001 at 12:59:02
---------------------------------------------------------------------------

Email: charlted-at-mcmaster.ca
Name: Emily Charlton

Organization: McMaster University

Education: Undergraduate College

Location: Hamilton, Ontario, CANADA

Question: I am wanting to find out better embedding techniques for
light microscopy which will allow me to remove a lot of the
chlorophyll present in the plant tissue but retain as much
anthocyanin as possible. I am currently trying to determine
anthocyanin location in a particular plant leaf. My first embedding
process involved using a 5% gluteraldehyde and phosphate buffer,
followed by a dehydration in increasing concentrations of ethanol and
then embedding using JB-4 epoxy resin kit. However, no anthocyanin
is present after the above process. Do you have any ideas of how I
might retain the anthocyanin, reduce the chlorophyll and observe the
pigment location using a light microscope. EM will be used at a
later date, but for now I am curious on how do do the above process.
Thanks for your help,
Emily Charlton,
McMaster University, CANADA

---------------------------------------------------------------------------


From daemon Tue Oct 23 07:55:49 2001



From: tbonner :      tbonner-at-brockport.edu
Date: Tue, 23 Oct 2001 07:48:23 -0500
Subject: Chairman & Professor Vacancy

Contents Retrieved from Microscopy Listserver Archives
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The Department of Biological Sciences at SUNY-Brockport is searching for a
Chairman. The vacancy announcement follows. We have an Hitachi 7000, several
ulytramicrotomes including an Ultracut S, digital darkroom and wet darkroom
facilities, along with several excellent LM with digital imaging systems.





Chair and Professor
SUNY – BROCKPORT
Department of Biological Sciences

The Department of Biological Sciences invites applications for a chair and
professor. Foremost, the Department is seeking an energetic, visionary chair
who will develop and enhance a balanced undergraduate major and Master’s
degree program. Required: Ph.D. in biology or related field; strong teaching,
scholarship, grant activity and administrative skills; ability to work
collegially in culturally diverse environment. The disciplinary expertise of
the candidates is a secondary consideration. In August 2001 the Department
moved into a newly renovated building ($12 million) with state-of-the-art
teaching and research facilities. Currently, the Department has strengths in
environmental sciences, cell/molecular biology, and vertebrate biology.

Submit letter of application, resume, transcript showing highest earned
degree, three letters of reference, and brief statements of leadership
philosophy, teaching philosophy, research plans and equipment requirements.

Salary is competitive. Starting date is August 2002. Beginning review date
for applications is December 15, 2001. Applications will be accepted until
January 15, 2002 or until the position is filled.

Send application materials to: Mr. Terry Hooper, Faculty/Staff Recruitment
Office, SUNY College at Brockport, 409 Allen Administration Building, 350 New
Campus Drive, Brockport, NY 14420-2929 AA/EOE

Dr. Thomas P. Bonner
Department of Biological Sciences
SUNY at Brockport
Brockport, NY 14420


From daemon Tue Oct 23 09:16:44 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 23 Oct 2001 10:05:13 -0400
Subject: Agfa 23D56 Scientia TEM Film

Contents Retrieved from Microscopy Listserver Archives
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We have been contacted by Agfa Corporation that they
have a small inventory ( approx 30 boxes) of Agfa Scientia
23D56 Electron Microscope film. This film is 3.25" x 4"
100 sheet boxes, with an expiration date of 12/02.
Agfa has said they will offer the film at a discount,
but prefers to sell it in one bundle.
If anyone is interested please contact me directly.

PS- If this is an improper use of this forum, I apologize.
It seemed like the quickest method to distribute this
information to those who may be interested.

Sincerely,

George

George Laing
National Graphic Supply
v:(518) 438-8411 X3109
v:(800) 223-7130 x3109 USA
f:(800) 832-2205
email: scisales-at-ngscorp.com




From daemon Tue Oct 23 10:19:59 2001



From: sghoshro-at-NMSU.Edu
Date: Tue, 23 Oct 2001 09:12:45 -0600 (MDT)
Subject: disposal of refrigerator

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

We are about to retire a full size refrigerator which had been used to
store osmium in both crystalline and liquid form for years. Our Safety
people are asking us to clean/decontaminate it before they remove it from
the lab. Is there any way to clean this ?

Thanks in advance.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml



From daemon Tue Oct 23 11:12:11 2001



From: J. Cindy Kleier :      j-kleier-at-northwestern.edu
Date: Tue, 23 Oct 2001 11:02:59 -0500
Subject: Micromanipulator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone is interested, I have a Narishige Joystick Micromanipulator,
model number MN151, for sale.  I am asking $1300 or best
offer.  It has been used very little and it is still in the
box.

If you are interested please e-mail me at j-kleier-at-northwestern.edu, off
the list serve.

Cindy



From daemon Tue Oct 23 11:28:18 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 23 Oct 2001 12:18:35 -0400
Subject: RE: microdissection before SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Morning Nami,
While the dimensions are somewhat less than optimal, the following
might offer two fruitful paths to try, and I emphasize might.
I am reminded of an old, tried-and-true, AND direct approach which
might help you quickly. Freeze drying of the unfixed organisms. Once the
organism is dried, retrieval of the statolith should be facilitated via
microdissection as long as it is not fractured by the freezing and drying
procedure.
While the above will probably enable isolation of the statolith, if
I were anxious to study a statolith (with those dimensions) and in ascidian
larvae, I would NOT try to isolate it, I would try to expose it in situ.
The easiest method by which one might accomplish that is to deep freeze the
entire structure, then cleave it with a single-edged razor blade, then look
at it directly. If some of my preparations exposed an intact statolith, I
would seek to isolate it after I had viewed it with the SEM and EDS/WDS.
For TEM, I would process the structure in situ and thin section it as I
would a piece of bone.
Getting back to freezing and fracturing, with the proper
equipment (VPSEM w/cleaving and cryotransfer attachment) you might be
rewarded with much valuable data. In the absence of such equipment, I would
concentrate first on the statolith which doesn't have to be preserved in the
same manner as surrounding tissue.
Now, how to accomplish this without expensive equipment. Since best
fractures occur at lower temperatures, you might place a piece (brick) of
steel (1" thick) in a small SS pan (NOT shallow) which is surrounded by the
foam filler one can acquire at Home Depot as an insulator. Pre-cool the
steel by pouring LN2 first over, then maintain it around the steel brick.
Deep freeze the larva or the statolith, place it on the brick in a drop of
an appropriate organic with a low freezing point to bind it to the brick,
and cleave the frozen statolith with a pre-cooled single-edge razor blade
held in a cooled pair of pliers held by your safely gloved hand. Tap the
back of the razor lightly, though simply putting it down might be
sufficient, to create a fracture, and hope for the best. NOTE: A member
of my undergraduate class suffocated (and died) in an evaporated LN2
atmosphere. Please be careful how you construct an open LN2 system with
which you must become somewhat intimate. Also, there are technical concerns
beyond safety with which you should be familiar, so I recommend you 'study
up' on the subject of freezing biological material for microscopic study
before you proceed (if you decide to try this).
You have asked for an approach, and I have suggested two. While the
confocal might very well provide you with some interesting information about
the hemisphere of surrounding tissue on the objective side of the organism,
there is far less chance of acquiring any internal information since the
statolith is not transparent and will thus be reflective no matter how you
embed it.
Good luck,

Fred Monson

} From:

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street

West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/

To:

} Nami Choe
} Ocean Science Centre
} Memorial University of Newfoundland
} St. John's, NF
} A1C 5S7 Canada
} phone:(709)737-3247
} fax:(709)737-3220
}
}
}
}
}


From daemon Tue Oct 23 16:18:50 2001



From: Xinran Liu :      xinran.liu-at-utsouthwestern.edu
Date: Tue, 23 Oct 2001 14:24:16 -0500
Subject: Need help on fluorescence recorded on Fujichrome film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, colleagues,

We sometime document our immunofluorescent images using regular SLR camera
and Fujichrome 400 slide film. The problem is that the red fluorescent
signal (568nm) did not show up bright on final developed film, However, the
green (488) and blue (350nm) ones are fine. We use Olympus BX51 microscopy
with a filter set of wide band (U-MWIY2)for red fluorescence.

Since the red signal is perfectly fine through binocular tube, I suspect
that either the problem is caused by development (chemical) or film itself.

Any help will be highly appreciated.

Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
UT Southwestern Medical Center
6000 Harry Hines Blvd., NA4.214
Dallas, TX 75390-9111

Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: Xinran.Liu-at-UTsouthwestern.edu



From daemon Tue Oct 23 17:47:16 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Wed, 24 Oct 2001 08:48:13 +1000
Subject: Re: disposal of refrigerator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If there is black discolouration inside, it is due to insoluble osmium
dioxide. You can possibly remove it by sponging with diluted hydrogen
peroxide. This will reoxidise the dioxide to the tetroxide which is more
soluble and can be washed off.
Of course, in doing this, you will be exposed to osmium tetroxide solution
and vapour, so you need protective clothing.

Otherwise you have to scrub off the dioxide with some kind of abrasive
cream cleaner (do you have JIF?). The dioxide is inert and relatively
harmless.

BTW adding hydrogen peroxide dropwise to osmium fixative which has gone
dark will reoxidise it to the tetroxide (and water) and restore it to
useful condition.
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Tue Oct 23 19:36:43 2001



From: Alan E Davis :      adavis-at-saipan.com
Date: Wed, 24 Oct 2001 10:23:32 +1000
Subject: Re: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
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I feel somewhat out of parameters on this list; I have had some thoughts,
however, about this topic.

I consider the work of the artist to open our eyes to new realities, to
new ways of seeing ourselves, our experiences, the world around us. The
artist is concerned to enhance our understanding of the world The
physical product of the artist's work are not art, but artifacts. I
think this definition of art is problematical, since there obviously is
some sense of art work as an aethetic object as well.

Is the work of the scientist not also to shed new light that will enhance
our understanding of the natural world?

Both are also concerned with sharing their work. Even the scientist who
is cloistered in his corner of the laboratory must eventually be concerned
to share new knowledge with others.

I wonder whether graphic data displays (as analytical tools) are objects
of art? They can certainly be admirable.

The scientist is concerned with knowledge, with factual truth. This is a
difference.

Another thought. I once read, though I don't remember where, a "Balinese
saying." I cannot verify its authenticity:

We have no art. We do everything the best we can.

Is scientific writing a form of literary art?

Thank you for allowing me to express these disconnected thoughts.

Alan Davis
Marianas High School
Saipan, N. Mariana Islands
adavis-at-saipan.com


From daemon Tue Oct 23 23:10:30 2001



From: Rick Webb :      r.webb-at-mailbox.uq.edu.au
Date: Wed, 24 Oct 2001 14:00:36 -0800
Subject: Nikon Coolscan 8000ED

Contents Retrieved from Microscopy Listserver Archives
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Hi

I have just seen the new Nikon Super Coolscan 8000ED demonstrated. It
was certainly very impressive. The 4000dpi and the density range of 4.2
make it a great scanner for EM negatives. I'm at present using a
LeafScan 45 and this new Nikon was equal to or better than it in all
areas and at a fraction of the cost. I have only a couple of
reservations about it. The only negative holders that we saw
demonstrated would not hold a standard (3 1/4 x 4 in , 8.8 x 10.2cm) TEM
negative. We were forced to cut down the negatives. Has anyone been able
to construct an adapter to take a full negative? I'm not too worried
about the fact that it won't scan the entire area of the negative but
don't like the idea of having to cut up negatives. It also seemed that
the construction of the negative carriers did not appear to be very
solid. I worry that in our multi user centre they may not last very
long.

I'd be keen to hear from anyone who has experience with this scanner

Thanks

Rick
--

===================================================
Rick Webb
Senior Research Officer
Department of Microbiology and Parasitology and
Centre for Microscopy and Microanalysis
University of Queensland 4072
Australia

Ph (+61)- 7-3365-1138
Fax (+61)- 7-3365-2199
==================================================


From daemon Wed Oct 24 02:09:11 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Wed, 24 Oct 2001 08:04:13 +0100
Subject: Re: disposal of refrigerator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Mel
I purchased some ruthenium tetroxide about a year ago, but the stock
went off quite quickly, and
the ampoules are now full of a black precipitate.
Can I titrate this against H2O2 in the same way ?
Chris

}
} BTW adding hydrogen peroxide dropwise to osmium fixative which has
gone
} dark will reoxidise it to the tetroxide (and water) and restore it
to
} useful condition.
} Dr. Mel Dickson,
} Deputy Director, The Electron Microscope Unit,
} Adjunct Associate Professor, School of Microbiology & Immunology
} The University of New South Wales
} UNSW SYDNEY 2052
} Australia.
} Phone +612 9385 6383 Fax +612 9385 6400
} http://srv.emunit.unsw.edu.au/
}



From daemon Wed Oct 24 03:42:17 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 24 Oct 2001 09:34:35 +0100 (GMT Daylight Time)
Subject: Re: RE: microdissection before SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most of us work with liquid nitrogen at some stage. Could
you tell us more about the circumstances in which someone
suffocated. I have up to now assumed small (1L) liquid
volumes in a lab were safe. Last year a scientist died in
Scotland. As far as I can tell a large volume of liquid
was involved.

Dave


On Tue, 23 Oct 2001 12:18:35 -0400 "Monson, Frederick C."
{fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Morning Nami,
} While the dimensions are somewhat less than optimal, the following
} might offer two fruitful paths to try, and I emphasize might.
} I am reminded of an old, tried-and-true, AND direct approach which
} might help you quickly. Freeze drying of the unfixed organisms. Once the
} organism is dried, retrieval of the statolith should be facilitated via
} microdissection as long as it is not fractured by the freezing and drying
} procedure.
} While the above will probably enable isolation of the statolith, if
} I were anxious to study a statolith (with those dimensions) and in ascidian
} larvae, I would NOT try to isolate it, I would try to expose it in situ.
} The easiest method by which one might accomplish that is to deep freeze the
} entire structure, then cleave it with a single-edged razor blade, then look
} at it directly. If some of my preparations exposed an intact statolith, I
} would seek to isolate it after I had viewed it with the SEM and EDS/WDS.
} For TEM, I would process the structure in situ and thin section it as I
} would a piece of bone.
} Getting back to freezing and fracturing, with the proper
} equipment (VPSEM w/cleaving and cryotransfer attachment) you might be
} rewarded with much valuable data. In the absence of such equipment, I would
} concentrate first on the statolith which doesn't have to be preserved in the
} same manner as surrounding tissue.
} Now, how to accomplish this without expensive equipment. Since best
} fractures occur at lower temperatures, you might place a piece (brick) of
} steel (1" thick) in a small SS pan (NOT shallow) which is surrounded by the
} foam filler one can acquire at Home Depot as an insulator. Pre-cool the
} steel by pouring LN2 first over, then maintain it around the steel brick.
} Deep freeze the larva or the statolith, place it on the brick in a drop of
} an appropriate organic with a low freezing point to bind it to the brick,
} and cleave the frozen statolith with a pre-cooled single-edge razor blade
} held in a cooled pair of pliers held by your safely gloved hand. Tap the
} back of the razor lightly, though simply putting it down might be
} sufficient, to create a fracture, and hope for the best. NOTE: A member
} of my undergraduate class suffocated (and died) in an evaporated LN2
} atmosphere. Please be careful how you construct an open LN2 system with
} which you must become somewhat intimate. Also, there are technical concerns
} beyond safety with which you should be familiar, so I recommend you 'study
} up' on the subject of freezing biological material for microscopic study
} before you proceed (if you decide to try this).
} You have asked for an approach, and I have suggested two. While the
} confocal might very well provide you with some interesting information about
} the hemisphere of surrounding tissue on the objective side of the organism,
} there is far less chance of acquiring any internal information since the
} statolith is not transparent and will thus be reflective no matter how you
} embed it.
} Good luck,
}
} Fred Monson
}
} } From:
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
}
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
} To:
}
} } Nami Choe
} } Ocean Science Centre
} } Memorial University of Newfoundland
} } St. John's, NF
} } A1C 5S7 Canada
} } phone:(709)737-3247
} } fax:(709)737-3220
} }
} }
} }
} }
} }
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Oct 24 08:51:50 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 24 Oct 2001 09:41:39 -0400
Subject: RE: microdissection before SEM/TEM -LN2 Hazard

Contents Retrieved from Microscopy Listserver Archives
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LARGE VOLUME, SMALL SPACE - Truck cab in winter. Truck had a leak.
Classmate closed windows of cab and simply went to sleep. Very sad and
avoidable. I have found graduate students (still alive) in small closed
office labs with open pans of LN2. They were unaware of the potential when
air circulation was not assured.

Sorry for the unspecified reference.

Fred Monson

} ----------
} From: Patton, David
} Sent: Wednesday, October 24, 2001 4:34 AM
} To: Monson, Frederick C.
} Cc: 'Microscopy Listserver'; 'Nami Choe'
} Subject: Re: RE: microdissection before SEM/TEM
}
} Most of us work with liquid nitrogen at some stage. Could
} you tell us more about the circumstances in which someone
} suffocated. I have up to now assumed small (1L) liquid
} volumes in a lab were safe. Last year a scientist died in
} Scotland. As far as I can tell a large volume of liquid
} was involved.
}
} Dave
}
}
} On Tue, 23 Oct 2001 12:18:35 -0400 "Monson, Frederick C."
} {fmonson-at-wcupa.edu} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Morning Nami,
} } While the dimensions are somewhat less than optimal, the following
} } might offer two fruitful paths to try, and I emphasize might.
} } I am reminded of an old, tried-and-true, AND direct approach which
} } might help you quickly. Freeze drying of the unfixed organisms. Once
} the
} } organism is dried, retrieval of the statolith should be facilitated via
} } microdissection as long as it is not fractured by the freezing and
} drying
} } procedure.
} } While the above will probably enable isolation of the statolith, if
} } I were anxious to study a statolith (with those dimensions) and in
} ascidian
} } larvae, I would NOT try to isolate it, I would try to expose it in situ.
} } The easiest method by which one might accomplish that is to deep freeze
} the
} } entire structure, then cleave it with a single-edged razor blade, then
} look
} } at it directly. If some of my preparations exposed an intact statolith,
} I
} } would seek to isolate it after I had viewed it with the SEM and EDS/WDS.
} } For TEM, I would process the structure in situ and thin section it as I
} } would a piece of bone.
} } Getting back to freezing and fracturing, with the proper
} } equipment (VPSEM w/cleaving and cryotransfer attachment) you might be
} } rewarded with much valuable data. In the absence of such equipment, I
} would
} } concentrate first on the statolith which doesn't have to be preserved in
} the
} } same manner as surrounding tissue.
} } Now, how to accomplish this without expensive equipment. Since best
} } fractures occur at lower temperatures, you might place a piece (brick)
} of
} } steel (1" thick) in a small SS pan (NOT shallow) which is surrounded by
} the
} } foam filler one can acquire at Home Depot as an insulator. Pre-cool the
} } steel by pouring LN2 first over, then maintain it around the steel
} brick.
} } Deep freeze the larva or the statolith, place it on the brick in a drop
} of
} } an appropriate organic with a low freezing point to bind it to the
} brick,
} } and cleave the frozen statolith with a pre-cooled single-edge razor
} blade
} } held in a cooled pair of pliers held by your safely gloved hand. Tap
} the
} } back of the razor lightly, though simply putting it down might be
} } sufficient, to create a fracture, and hope for the best. NOTE: A
} member
} } of my undergraduate class suffocated (and died) in an evaporated LN2
} } atmosphere. Please be careful how you construct an open LN2 system with
} } which you must become somewhat intimate. Also, there are technical
} concerns
} } beyond safety with which you should be familiar, so I recommend you
} 'study
} } up' on the subject of freezing biological material for microscopic study
} } before you proceed (if you decide to try this).
} } You have asked for an approach, and I have suggested two. While the
} } confocal might very well provide you with some interesting information
} about
} } the hemisphere of surrounding tissue on the objective side of the
} organism,
} } there is far less chance of acquiring any internal information since the
} } statolith is not transparent and will thus be reflective no matter how
} you
} } embed it.
} } Good luck,
} }
} } Fred Monson
} }
} } } From:
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging(CASI)
} } West Chester University of Pennsylvania
} } Schmucker Science Center II
} } South Church Street
} }
} } West Chester, PA, 19383
} } eMail: fmonson-at-wcupa.edu
} } http://darwin.wcupa.edu/casi/
} }
} } To:
} }
} } } Nami Choe
} } } Ocean Science Centre
} } } Memorial University of Newfoundland
} } } St. John's, NF
} } } A1C 5S7 Canada
} } } phone:(709)737-3247
} } } fax:(709)737-3220
} } }
} } }
} } }
} } }
} } }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}


From daemon Wed Oct 24 09:56:44 2001



From: Wiggins, Winston :      Winston.Wiggins-at-carolinashealthcare.org
Date: Wed, 24 Oct 2001 10:33:56 -0400
Subject: TEM:CM10 Specimen Holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers,
If anyone has available for sale a manual single or double-tilt specimen
holder fitting the Philips CM 10/12 series, please contact me with a price
quote and condition of holder.
Thanks,
Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, Supervisor 10/24/2001 10:29 AM
Cannon Electron Microscopy Lab Ofc: 704-355-1267
Carolinas Medical Center Lab: 704-355-7220
P.O. Box 32861 (Ship to: 1000 Blythe Blvd ) Fax: 704-355-0589
Charlotte, NC 28232-2861 (Ship to: 28203 )
Winston.Wiggins-at-CarolinasHealthCare.org {mailto:WWiggins-at-Carolinas.org}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
"Vocatus atque non vocatus, Deus aderit." - C.G.Jung



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From daemon Wed Oct 24 11:10:13 2001



From: Jim Haley :      haley-at-mvia.com
Date: Wed, 24 Oct 2001 11:59:17 -0400
Subject: Re: LM: The Coolpix - Nikon Optiphot Connection

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Tom,

I'm not sure exactly what info you're looking for, but we have adapters
for the Coolpix to mount to just about any microscope. On the Nikon
microscopes we have 3 options: a 23 mm phototube, a 30 mm phototube and
a 38 mm ISO photoport. Give me a call and we can go over the specifics
of your scope.

Thanks,
Jim Haley

******************************
Jim Haley
Applications Engineer
MVIA, Inc.
12901 Duckettown Road
Laurel, MD 20708
voice: (301) 809-6292
fax: (301) 805-2819
e-mail: haley-at-mvia.com
webpage: http://www.mvia.com
******************************

Tom Tottleben wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} To: Great Minds in the Halls of Science Wisdom!
}
} I realize this topic has been hammered out in this forum a few times
} (for everything except the Nikon Optiphot superwide
} trinocular) ......can someone direct me to any information that would be
} helpful? ("I can do this .......yeah!!!") Thanks,
}
} Tom Tottleben
} Tottleben Scientific Co
} PO Box 24
} 104 Burns Farm-W.Ct.
} Edwardsville, IL. 62025
} 618 656 9008 Office
} 618 656 9599 Fax
} 618 558 9008 Cell
} 800 283 9997 Orders
} tomtot-at-charter.net
} www.tscmicroscopes.com


From daemon Wed Oct 24 12:56:29 2001



From: =?iso-8859-1?q?Valeria=20L=20Burgos?= :      valaburg-at-yahoo.com.ar
Date: Wed, 24 Oct 2001 14:48:28 -0300 (ART)
Subject: neural tissue - antibody search

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello all!
for those who work (or starting to work, that`s my case) on Bain
tissue research:
I`m looking for an rat/mouse antibody anti-NCAM and anti-PSA-NCAM.
I recently purchased two antibodies from the Developmental Studies
Hybridoma Bank of the U of Iowa, but those abs are anti-embrionic
form of rat. I am looking for an `adult`rat/mouse form.

Please, I would appreciate your help

:)

Valeria L.Burgos
Instituto de Ciencias Bàsicas y Medicina Experimental
Hospital Italiano de Buenos Aires - ARGENTINA
Tel: 54-11-9590200 extension 8919

_________________________________________________________
¿Lo probaste?
Correo gratis y para toda la vida en http://correo.yahoo.com.ar


From daemon Wed Oct 24 13:26:33 2001



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Wed, 24 Oct 2001 14:19:42 -0400
Subject: alignment of ion sources in Gatan DuoMill

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

I will appreciate to get any information on alignment of ion sources in
Gatan DuoMill

Jinguo Wang


Jinguo Wang, Ph.D
The Pennsylvania State University
Materials Research Institute
194 Materials Research Institute Building
University Park, PA 16802
Tel: (814) 865-9285
Fax: (814) 863-8561, (814) 863-0637
email: jqw11-at-psu.edu


From daemon Wed Oct 24 13:35:56 2001



From: Ed Monberg :      em1-at-lasermotion.com
Date: Wed, 24 Oct 2001 11:30:30 -0700
Subject: Opinions on Panasonic 3CCD Cameras?

Contents Retrieved from Microscopy Listserver Archives
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I am wondering if there are folks who have used the Panasonic GP-US502 1/2"
3CCD camera and its successor the GP-US522 ?

We are thinking of integrating them on some microscopes.

Have you found GP-US502 sensitive enough to capture fluorescence images ?

Criticisms ?
Prices Paid ?
Thoughts ?

Thanks,


Sincerely,

Ed Monberg, General Manager
LMDC
3101 Whipple Road, Union City, CA 94587
510-429-1060, Fax 429-1065, Cell 510-427-0115
WEB CATALOGUE: http://www.lasermotion.com
---------------------------------------------




From daemon Wed Oct 24 14:19:13 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 24 Oct 2001 15:09:06 -0400
Subject: Re: Microdissection and a Potential LN2 Hazard?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To All who are concerned about my earlier LN2 reference.

In an earlier recommendation to the originator of the question about
statoliths, I cautioned in my suggestions that a friend had died in an LN2
accident. Apparently there is some concern that I was referring to a death
by a small amount of LN2. No one knows what volumes were involved, but the
next paragraph should clarify the matter.

I didn't say that my friend died from a liter or, I think, suggest
that the young lady could. My fraternity brother did die from asphyxiation
from a nitrogen leak while he was trying to fix it in the closed cab of a
tanker truck - according to the company AND the coroner at the time. We
never could figure out why he was working in the cab or how there could have
been a leak to fix in such a location. I even asked if it was the cab or
the tank, and it WAS the cab. [A pressure line to the cab to monitor
pressure in the tank? In their O2 carriers as well?????] All other info
was lawyer'd quiet!

I never had a problem with a liter either, but I have recommended
that students not work alone with any amount of LN2 in closed rooms with
poor or no ventilation even if I couldn't specify the specific danger. I've
never felt even drowsy either, but I haven't dealt with large-volume
evolutions.

I did have an assistant once who loaded a 20L dewar in a small
unventilated closet in which the 160L dewer was kept. When she came out,
she claimed to feel faint. When I checked the dewar, it had lost half its
contents in two days, and there appeared to be a leak in the siphon valve.
I had it moved to a corner in a larger, well-ventilated room once the valve
was replaced. Never had a problem thereafter, even when there were leaks.

In the final analysis, however, when I suggest the use of LN2 to
people I don't know who work in environments with which I'm not familiar, I
want a little "CYA" on my side.

Sorry I raised a red flag by an obscure reference to death. Perhaps
it was ill-advised or at least poorly represented. I will not forget the
concerns.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



From daemon Wed Oct 24 22:16:34 2001



From: 01151938-at-mrc.vic.edu.au
Date: Wed, 24 Oct 2001 22:02:13 -0500
Subject: Interaction between art and science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers
I wish to sincerely thank everyone who responded to my question.This
has opened more doors for me to explore.I am grateful for your
generosity, in particular providing me with references and email
contacts.
Kind regards
Judi Bowden


From daemon Thu Oct 25 03:02:54 2001



From: Ian Kaplin :      I.Kaplin-at-emu.usyd.edu.au
Date: Thu, 25 Oct 2001 17:50:33 +1000
Subject: TEM: Need Philips Strain Holder

Contents Retrieved from Microscopy Listserver Archives
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Can anyone lend the University of Sydney EM Unit (for a short time) a
strain holder suitable for use with a Philips 400 (or CM12) series
microscope.

Ian Kaplin Tel : 61 2 9351 3302 Fax : 61 2 9351 7682
Electron Microscope Unit, Madsen Bldg F09
The University of Sydney, NSW 2006
Australia


From daemon Thu Oct 25 05:00:21 2001



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Thu, 25 Oct 2001 10:50:09 +0000 (GMT)
Subject: Re: Ni Films

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Roy,
three easy ways to tell the difference between Si and GaAs:

1) GaAs is not as strong as Si. So GaAs wafers are generally thicker than Si wafers of the same diameter and are easier to break.
2) GaAs is a lot denser than Si. So if you have two wafers the same diameter but one is noticeably heavier (also because of (1)), it's GaAs.
3) GaAs cleaves on (110) and Si cleaves on (111). If your substrate has been cleaved up and has a flat edge perpendicular to the top surface, it's GaAs. If the edge orientation varies and is rarely perpendicular, it's Si.

Hope this helps!

Richard


} From: Beavers, Roy
} Sent: Tuesday, October 23, 2001 4:44 PM
} To: Microprobe List (E-mail)
} Subject: Ni Films
}
} List,
}
} You have help me with issues before, which is greatly appreciated, so I
} would like to ask help on the latest problem I have been working on. I
} have
} been looking at some 500 angstrom Ni films on Si substrates trying to
} determine if there is any W contamination in the film.
}
} At first I thought I would use an beam of ~7KeV to keep my analysis within
} the film as much as possible but I realized I would probably not see the
} Ni
} or W X-ray lines with this set up so I decided to go with a beam of 15KeV
} which is where I usually run just to see what came up.
}
} Next I checked a Ni and W standard to see what lines I would get. This
} looked o.k. so I proceeded to check a sample of the source material and as
} expected I got a match for Ni and only Ni.
}
} Going to the film samples I expected to see Ni and I did, also I see no W,
} but I did expect to see Si because of the higher beam energy but I did
} not.
} This is my first question why no Si lines?
}
} What I did see on the film samples that was unexpected was what I believed
} to be lines for Ga and As. This may answer my question as to why no Si
} signal but I want to check my thoughts on this before I tell my customer
} they have deposited films on the wrong substrates.
}
} Any thoughts again will be greatly appreciated.
}
} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356365
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or
entity named above. If the reader of this message is not the
intended recipient, you are hereby notified that any dissemination,
distribution or copying of this message is strictly prohibited.
If you have received this message in error, please notify us
immediately by telephone."
==============================================================
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Registered in England No. 3694360
Registered Office: One Bruton Street London W1J 6AQ





From daemon Thu Oct 25 07:35:35 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 25 Oct 2001 07:23:23 -0500
Subject: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for advice or anecdotes about moving
heavy SEM equipment. I'm hoping to take possession
of an old SEM, whose column base is 33"x33" and may
weigh as much as 1,200 pounds. The console is
wider and may weigh only 300 pounds.

If I could avoid $1,000 on professional movers and
wrestle it with a few strong guys, that would be great,
but there are other questions in my mind.

Regardless of who moves it, what considerations must
they take regarding the sensitive nature of the
equipment? Certainly sensitive bits like filaments
should be removed and handled separately, right?

And what about the requirements for trucking?
If it's going to spend two or three hours on the
interstate, will any sort of truck do the job?

- John



From daemon Thu Oct 25 08:45:03 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 25 Oct 2001 09:27:36 -0400
Subject: Potential LN2 Hazard?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
To add to Fred's line about potential hazards involved with large
volumes of LN2...
Last spring, during the final stages of installing a new MRI facility
here at the hospital a young man from the company doing the
installation was killed by a large volume LN2 leak. I guess the
coils of those beasts are cooled and there was a leak in one of the
cooling lines and the room was filled with N2 gas...he was
asphyxiated immediately.
As long as our tanks & valves don't leak, the small volumes we
actually deal with on a daily basis are probably not a problem, but I
instruct everyone to work in a large room with good ventilation, or
to leave the door open. I've had the ventilation in my EM room
checked, since that is where my tank is stored.

lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Oct 25 08:45:03 2001



From: Norman C Miller :      Norman_C_Miller-at-raytheon.com
Date: Thu, 25 Oct 2001 08:37:12 -0500
Subject: used WDS electronics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

I have a used Microspec 2A electronics and computer that are no longer
needed. I will part with them to anyone who will take care of shipping
them.

Carl Miller


From daemon Thu Oct 25 09:41:17 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Thu, 25 Oct 2001 10:35:00 -0400
Subject: TEM simulation softwares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are thinking to buy softwares which are good in both high resolution TEM
image simulation and dynamical SAD and CBED diffraction simulation.

Can anyone recommend us some good ones?

Best Regards,


Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Thu Oct 25 12:29:12 2001



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Thu, 25 Oct 2001 10:19:25 -0700
Subject: LM:Ring light transformer to donate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a transformer for a Bausch and Lomb ring illuminator that I wish to
donate to any public institution willing to pay the shipping charge ($
5-10). I believe this works with the fluorescent ring illuminator for the
stereozoom series. The electrical specifications are "PRIMARY: 115 V AC-60
cycles SECONDARY: 750 V 45 milliamps". It appears to be in good working
condition (i.e. my voltmeter reads a 750 V output). I can send a digital
image on request.
Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Thu Oct 25 15:00:16 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 25 Oct 2001 15:49:02 -0400
Subject: Nikon Coolscan 8000ED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,
Many of our customers are very happy with the 8000ED.
I have looked into putting TEM negs into it and it could be
done by modifying one of the 120/220 film holders.
The holders have a raised lip to keep the film in the
channel. I believe these could be removed and the film could then
just extend out past the scan opening. I have not been able to try
this as demand for the scanner has been very high.
Another excellent scanner for TEM negs is the Agfa
T2500 Duoscan. While lower in resolution(2500dpi optical) it has
a glassless carrier design that will enable scanning of an entire
TEM negative. It also will scan reflective originals.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com

-----Original Message-----
} From: Rick Webb [mailto:r.webb-at-mailbox.uq.edu.au]
Sent: Wednesday, October 24, 2001 6:01 PM
To: Microscopy Listserver


Hi

I have just seen the new Nikon Super Coolscan 8000ED demonstrated. It
was certainly very impressive. The 4000dpi and the density range of 4.2
make it a great scanner for EM negatives. I'm at present using a
LeafScan 45 and this new Nikon was equal to or better than it in all
areas and at a fraction of the cost. I have only a couple of
reservations about it. The only negative holders that we saw
demonstrated would not hold a standard (3 1/4 x 4 in , 8.8 x 10.2cm) TEM
negative. We were forced to cut down the negatives. Has anyone been able
to construct an adapter to take a full negative? I'm not too worried
about the fact that it won't scan the entire area of the negative but
don't like the idea of having to cut up negatives. It also seemed that
the construction of the negative carriers did not appear to be very
solid. I worry that in our multi user centre they may not last very
long.

I'd be keen to hear from anyone who has experience with this scanner

Thanks

Rick
--

===================================================
Rick Webb
Senior Research Officer
Department of Microbiology and Parasitology and
Centre for Microscopy and Microanalysis
University of Queensland 4072
Australia

Ph (+61)- 7-3365-1138
Fax (+61)- 7-3365-2199
==================================================




From daemon Thu Oct 25 16:06:49 2001



From: Dave Campbell :      dcampbel-at-US.ibm.com
Date: Thu, 25 Oct 2001 16:58:19 -0400
Subject: Low Volumn Of LN2 and time factor.

Contents Retrieved from Microscopy Listserver Archives
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To All:
I've been wondering whether to respond or not but here is a situation that
happened in Vermont about 10 years ago. An artificial breeder was
asphyxiated and died when he fell asleep in the pickup bed of his truck. He
had his semen in a 5 - 10 gal LN2 Cryogenic Dewar that was in the bed of
his pickup truck. I don't think he had a cab over the bed. The conditions
were; warm/hot afternoon in Vermont (~80 degrees) cold for Texas. I believe
the Breeder wanted to take a nap in the back truck bed. The Cold Nitrogen
filled the truck bed and deplaced the Oxygen. The winds were minimal. He
was overcome by a relative small amount of LN2 but there was a time factor.
I believe they found him a few hours later. Beer may have also played a
role.

Bottom Line: Don't sleep on the floor of the Lab if your using LN2

Dave Campbell
IBM Micro-Electronics
Essex Junction, Vt.

P.S. I was introduced to the Darwin Awards on this forum, but haven't seen
any in a few years. Are there any updates? Please Respond off line.



From daemon Thu Oct 25 16:10:00 2001



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Thu, 25 Oct 2001 17:03:58 -0500
Subject: Re: Potential LN2 Hazard?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would not try to guess on this one. The only real solution is to
have an oxygen monitor. They can be a pain because they are now
required to be set at our facility to 19.5% for the alarm, so small
spills can trip them. They also require weekly maintenance. I'd
rather have the small annoyance of false alarms than the one time
chance of being dead.



At 9:27 AM -0400 10/25/2001, Leona Cohen-Gould wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} To add to Fred's line about potential hazards involved with large
} volumes of LN2...
} Last spring, during the final stages of installing a new MRI
} facility here at the hospital a young man from the company doing the
} installation was killed by a large volume LN2 leak. I guess the
} coils of those beasts are cooled and there was a leak in one of the
} cooling lines and the room was filled with N2 gas...he was
} asphyxiated immediately.
} As long as our tanks & valves don't leak, the small volumes we
} actually deal with on a daily basis are probably not a problem, but
} I instruct everyone to work in a large room with good ventilation,
} or to leave the door open. I've had the ventilation in my EM room
} checked, since that is where my tank is stored.
}
} lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175


--
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov
http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html


From daemon Thu Oct 25 16:20:53 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 25 Oct 2001 14:13:10 -0700
Subject: Re: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
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Dear John,
About two years ago I purchased a used SEM across the country and the movers
only charged me about $500 to move it all that way. I chose a firm that
delivers new SEMs, so they know what to do. You might ask your nearest SEM
service provider for references.
The microscope must be disassembled for shipping and the shipping bolts put
in to stop things from shaking free. There may be some instructions with the
documentation but at the least:
1. Remove the gun, cable and the connection into the high voltage tank and
wrap up separately. Remove and box rotary pumps, hoses, power supplies and
other connected pieces.
2. Label and disconnect all wires that stretch between the two sections.
3. Bolt down the column section.
My moving company did not box the SEM, just put it on two pallets and
wrapped it in plastic and moved everything, as much as possible, with a fork
lift. Two strong men had no problem maneuvering the column section off the
pallet and into the right place. If you think the column section is too
heavy, you can probably take the lenses off, as well.
This is a professional job to do right and IMHO the risk is worth getting an
SEM service professional to pack it up and a scientific instrument moving
company to move it is well worth what they charge. Make sure they have an
"air-bed" truck for moving.
At 07:23 AM 10/25/01 -0500, you wrote:
}
} I'm looking for advice or anecdotes about moving
} heavy SEM equipment. I'm hoping to take possession
} of an old SEM, whose column base is 33"x33" and may
} weigh as much as 1,200 pounds. The console is
} wider and may weigh only 300 pounds.
}
} If I could avoid $1,000 on professional movers and
} wrestle it with a few strong guys, that would be great,
} but there are other questions in my mind.
}
} Regardless of who moves it, what considerations must
} they take regarding the sensitive nature of the
} equipment? Certainly sensitive bits like filaments
} should be removed and handled separately, right?
}
} And what about the requirements for trucking?
} If it's going to spend two or three hours on the
} interstate, will any sort of truck do the job?
}
} - John
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Oct 25 16:25:07 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 25 Oct 2001 17:18:12 -0400 (EDT)
Subject: Re: microdissection before SEM/TEM

Contents Retrieved from Microscopy Listserver Archives
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I don't know anything about the stuctures you mention; however if you
could work from a slice of tissue (e.g., for Xray), check out these
publications:

Howell DN, Miller SE. Identification of viral infection by confocal
microscopy. 1999. Methods in Enzymology. Academic Press, 307:573-91.

Miller SE, Levenson RM, Aldridge C, Hester S, Kenan DJ, Howell DN.
1997. Identification of focal viral infections by confocal microscopy
for subsequent ultrastructural analysis. Ultrastruc Pathol 21:183-193.


Briefly, we make "vibratome" slices; stain them with propidium iodide,
examine them by confocal microscopy for unusual areas, and then cut out
the area of interest. Alternatively, if you have an antibody against the
structure of interest, you can use a fluorochrome to label them. In t
his manner, we've been able to locate a single virus-infected cell by
confocal and then find that same cell by EM.

Good luck,
Sara

On Mon, 22 Oct 2001, Nami Choe wrote:

} Date: Mon, 22 Oct 2001 15:37:43 -0230 (NDT)
} From: Nami Choe {c56nc-at-morgan.ucs.mun.ca}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: microdissection before SEM/TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear microscopy members;
}
} Hello. I am a marine biology graduate student at Memorial University of
} Newfoundland, Canada. One of my research topic is to study the
} microstructure of the statolith in the pelagic marine tunicate (Larvacea),
} Oikopleura vanhoeffeni. The statolith is a small cylindrical calcium
} build up (10 micrometer diameter, 20 micrometer height), attached to the
} brain of the tunicate. This bony structure is contained in the
} membraneous statocyst and probably works as a gravity detector.
} I would like to isolate this structure for SEM and TEM analyses. After
} this, Xray microcroprobe and other procedures will follow.
}
} Dissecting the brain is easy but taking out the statolith from the
} statocyst is the difficult part. Visualizing the statolith can be done by
} calcium specific stain, alizarin red. I have tried to dissect the
} statolith out by using fine insect pins ( { 20 micron tip size), but the
} pins are too large. Doing this under dissecting scope is impossible since
} 4x magnification does not allow me to do such microdissection.
}
} I tried to dissolve the statocyst with bleach and hydrogen peroxide to
} isolate inorganic statolith. However, after this procedure, I can no
} longer visualize the statolith since alizarin red stain dissolves
} instantaneously in these chemicals.
}
} Sonication was also done to rupture the statocyst and pop the statolith
} out but it did not work.
}
} Do you have any suggestion on how I can separate this structure?
} Sincerely,
} Nami Choe
} ____________________________________________________________________________
}
} Nami Choe
} Ocean Science Centre
} Memorial University of Newfoundland
} St. John's, NF
} A1C 5S7 Canada
} phone:(709)737-3247
} fax:(709)737-3220
}
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Thu Oct 25 17:50:22 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 25 Oct 2001 15:42:19 -0500
Subject: SymParter for LKB Knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would the users of the SymParter attachment for the LKB knifebreaker
care to comment on how useful it is? If you want to do it
anonymously, e-mail me directly and I will post the replies without
attribution. Thanks, Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Thu Oct 25 19:35:57 2001



From: Paul B. Grover :      pbgrover-at-home.com
Date: Thu, 25 Oct 2001 19:30:06 -0700
Subject: Re: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
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on 10/25/01 5:23 AM, John Foust at jfoust-at-threedee.com wrote:

} I'm looking for advice or anecdotes about moving
} heavy SEM equipment.

I've moved three SEMs, one of them locally, and two of them across-country
towed behind (uncovered, but contents well-tarped) rental trailers . My
experience has been that hiring SEM engineers is totally unnecessary.

From daemon Fri Oct 26 03:08:02 2001



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 26 Oct 2001 08:53:11 +0200
Subject: TEM of semiconducting materials: the Multiprep system

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Dear colleagues,

our group works on Transmission Electron Microscopy of semiconducting
materials, especially low-dimensional materials for optoelectronic
applications. We work on InGaAs/GaAs single and stacked quantum dots and we
recently started a new research activity on GaN on sapphire and/or Alumina
substrates.

We have a good experience with TEM sample preparation, but we recently
decided to buy the Multiprep System, to exploit new methods for TEM sample
preparation and to minimize the ion milling time.

We just assembled and calibrated the machine, and we are now in the
progress of preparing the first plan-view and cross-sectional samples.

As our machine is one of the few presently available in Italy, we are in
contact with Allied to have suggestions and directions.

I should appreciate it very much if those of you who have operated the
machine can give us suggestions, hints and procedures to get good quality
samples with this nice machine.

Thanks in advance for your help

Massimo

Dr. Massimo Catalano
IME-CNR
Via Arnesano
73100 Lecce - ITALY
phone: +39 0832 391199
fax: +39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it



From daemon Fri Oct 26 03:08:03 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 26 Oct 2001 08:17:18 +0100
Subject: Re: Potential LN2 Hazard?

Contents Retrieved from Microscopy Listserver Archives
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David
this is how it should be done, but there is no point in using a
monitor if it is disabled. A feature of the 1999 Scottish fatality was
that the deceased habitually disabled the oxygen monitor. Having said
that, we have operated without an oxygen monitor for years. Now may be
a good time to buy one ....
The circumstances of the Scottish fatality were somewhat
unrepresentative of lab conditions in that having become unconscious,
the victim was exposed to an effectively endless stream of nitrogen
delivered from a large-volume dewar. My department responded to that
incident by making it a rule that the person delivering nitrogen from
a storage dewar should always be accompanied by a colleague. That way,
there is a chance that someone can raise an alarm if things go
pear-shaped.

Chris

} I would not try to guess on this one. The only real solution is to
} have an oxygen monitor. They can be a pain because they are now
} required to be set at our facility to 19.5% for the alarm, so small
} spills can trip them. They also require weekly maintenance. I'd
} rather have the small annoyance of false alarms than the one time
} chance of being dead.
}
}
}
} At 9:27 AM -0400 10/25/2001, Leona Cohen-Gould wrote:
}
} ---------------------------------------------------------------------
---
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} ---------------------------------------------------------------------
--.
} }
} }
} } Hi All,
} } To add to Fred's line about potential hazards involved with large
} } volumes of LN2...
} } Last spring, during the final stages of installing a new MRI
} } facility here at the hospital a young man from the company doing
the
} } installation was killed by a large volume LN2 leak. I guess the
} } coils of those beasts are cooled and there was a leak in one of the
} } cooling lines and the room was filled with N2 gas...he was
} } asphyxiated immediately.
} } As long as our tanks & valves don't leak, the small volumes we
} } actually deal with on a daily basis are probably not a problem, but
} } I instruct everyone to work in a large room with good ventilation,
} } or to leave the door open. I've had the ventilation in my EM room
} } checked, since that is where my tank is stored.
} }
} } lee
} } --
} } Leona Cohen-Gould, M.S.
} } Sr. Staff Associate
} } Director, Electron Microscopy Core Facility
} } Manager, Optical Microscopy Core Facility
} } Joan & Sanford I. Weill Medical College
} } of Cornell University
} } voice (212)746-6146
} } fax (212)746-8175
}
}
} --
} David R. Hull
} NASA Glenn Research Center at Lewis Field
} Advanced Metallics Branch
} Mail Stop 49-1
} 21000 Brookpark Road
} Cleveland, OH 44135
}
} (216) 433-3281
} fax (216)977- 7132
} david.r.hull-at-grc.nasa.gov
} http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html
}



From daemon Fri Oct 26 05:42:02 2001



From: Ray Hicks :      rh208-at-cam.ac.uk
Date: Fri, 26 Oct 2001 11:31:45 +0100
Subject: Re: Potential LN2 Hazard?

Contents Retrieved from Microscopy Listserver Archives
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Even better, forced ventilation so that accumulation is impossible, and an
alarm for when the impossible happens.

Ray
} From: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
} Organization: Inveresk Cottage
} Reply-To: "Chris Jeffree" {c.jeffree-at-ed.ac.uk}
} Date: Fri, 26 Oct 2001 08:17:18 +0100
} To: "David R Hull" {David.R.Hull-at-grc.nasa.gov}
} Cc: {microscopy-at-sparc5.microscopy.com}
} Subject: Re: Potential LN2 Hazard?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} David
} this is how it should be done, but there is no point in using a
} monitor if it is disabled. A feature of the 1999 Scottish fatality was
} that the deceased habitually disabled the oxygen monitor. Having said
} that, we have operated without an oxygen monitor for years. Now may be
} a good time to buy one ....
} The circumstances of the Scottish fatality were somewhat
} unrepresentative of lab conditions in that having become unconscious,
} the victim was exposed to an effectively endless stream of nitrogen
} delivered from a large-volume dewar. My department responded to that
} incident by making it a rule that the person delivering nitrogen from
} a storage dewar should always be accompanied by a colleague. That way,
} there is a chance that someone can raise an alarm if things go
} pear-shaped.
}
} Chris
}
} } I would not try to guess on this one. The only real solution is to
} } have an oxygen monitor. They can be a pain because they are now
} } required to be set at our facility to 19.5% for the alarm, so small
} } spills can trip them. They also require weekly maintenance. I'd
} } rather have the small annoyance of false alarms than the one time
} } chance of being dead.
} }
} }
} }
} } At 9:27 AM -0400 10/25/2001, Leona Cohen-Gould wrote:
} }
} } ---------------------------------------------------------------------
} ---
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe -- Send Email to
} } } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} } ---------------------------------------------------------------------
} --.
} } }
} } }
} } } Hi All,
} } } To add to Fred's line about potential hazards involved with large
} } } volumes of LN2...
} } } Last spring, during the final stages of installing a new MRI
} } } facility here at the hospital a young man from the company doing
} the
} } } installation was killed by a large volume LN2 leak. I guess the
} } } coils of those beasts are cooled and there was a leak in one of the
} } } cooling lines and the room was filled with N2 gas...he was
} } } asphyxiated immediately.
} } } As long as our tanks & valves don't leak, the small volumes we
} } } actually deal with on a daily basis are probably not a problem, but
} } } I instruct everyone to work in a large room with good ventilation,
} } } or to leave the door open. I've had the ventilation in my EM room
} } } checked, since that is where my tank is stored.
} } }
} } } lee
} } } --
} } } Leona Cohen-Gould, M.S.
} } } Sr. Staff Associate
} } } Director, Electron Microscopy Core Facility
} } } Manager, Optical Microscopy Core Facility
} } } Joan & Sanford I. Weill Medical College
} } } of Cornell University
} } } voice (212)746-6146
} } } fax (212)746-8175
} }
} }
} } --
} } David R. Hull
} } NASA Glenn Research Center at Lewis Field
} } Advanced Metallics Branch
} } Mail Stop 49-1
} } 21000 Brookpark Road
} } Cleveland, OH 44135
} }
} } (216) 433-3281
} } fax (216)977- 7132
} } david.r.hull-at-grc.nasa.gov
} } http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html
} }
}
}
}



From daemon Fri Oct 26 06:18:42 2001



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 26 Oct 2001 12:12:21 +0100 (GMT Daylight Time)
Subject: Re: Potential LN2 Hazard: re oxygen monitors

Contents Retrieved from Microscopy Listserver Archives
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I believe that in the case in Scotland the oxygen monitors
were either switched off or ignored.

Dave


On Thu, 25 Oct 2001 17:03:58 -0500 David R Hull
{David.R.Hull-at-grc.nasa.gov} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would not try to guess on this one. The only real solution is to
} have an oxygen monitor. They can be a pain because they are now
} required to be set at our facility to 19.5% for the alarm, so small
} spills can trip them. They also require weekly maintenance. I'd
} rather have the small annoyance of false alarms than the one time
} chance of being dead.
}
}
}
} At 9:27 AM -0400 10/25/2001, Leona Cohen-Gould wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} } To add to Fred's line about potential hazards involved with large
} } volumes of LN2...
} } Last spring, during the final stages of installing a new MRI
} } facility here at the hospital a young man from the company doing the
} } installation was killed by a large volume LN2 leak. I guess the
} } coils of those beasts are cooled and there was a leak in one of the
} } cooling lines and the room was filled with N2 gas...he was
} } asphyxiated immediately.
} } As long as our tanks & valves don't leak, the small volumes we
} } actually deal with on a daily basis are probably not a problem, but
} } I instruct everyone to work in a large room with good ventilation,
} } or to leave the door open. I've had the ventilation in my EM room
} } checked, since that is where my tank is stored.
} }
} } lee
} } --
} } Leona Cohen-Gould, M.S.
} } Sr. Staff Associate
} } Director, Electron Microscopy Core Facility
} } Manager, Optical Microscopy Core Facility
} } Joan & Sanford I. Weill Medical College
} } of Cornell University
} } voice (212)746-6146
} } fax (212)746-8175
}
}
} --
} David R. Hull
} NASA Glenn Research Center at Lewis Field
} Advanced Metallics Branch
} Mail Stop 49-1
} 21000 Brookpark Road
} Cleveland, OH 44135
}
} (216) 433-3281
} fax (216)977- 7132
} david.r.hull-at-grc.nasa.gov
} http://www.lerc.nasa.gov/WWW/AdvMet/ASGWEB2000/asghome.html
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Fri Oct 26 07:05:47 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 26 Oct 2001 07:59:14 -0400
Subject: Re: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
You didn't state what kind of SEM. I've moved MANY ETECs with a 5x8
covered U-Haul trailer. I built a 10' folding ramp specifically for
these low bed trailers and support the center with a couple of scissors
jacks. I prefer to transport them under vacuum because the column is
small enough to fit fully assembled and doesn't have a whole lot of
mass. The optics table needs to be bolted down, as Mary said, and the
high voltage supply handled separately, etc. The chief advantage on
these is that they're on wheels and one person can do a move.

For systems that aren't on wheels (almost all others) you can rent a
Johnson Bar (pry bar with wheels) and a safe jack (pair of 2 wheeled
fork lift units that are strapped to the thing you want to move) and do
it yourself (with a friend). You might want to consider a truck with a
lift-gate and air suspension. Make sure things are well secured before
you drive anywhere.

Good luck!

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

John Foust wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I'm looking for advice or anecdotes about moving
} heavy SEM equipment. I'm hoping to take possession
} of an old SEM, whose column base is 33"x33" and may
} weigh as much as 1,200 pounds. The console is
} wider and may weigh only 300 pounds.
}
} If I could avoid $1,000 on professional movers and
} wrestle it with a few strong guys, that would be great,
} but there are other questions in my mind.
}
} Regardless of who moves it, what considerations must
} they take regarding the sensitive nature of the
} equipment? Certainly sensitive bits like filaments
} should be removed and handled separately, right?
}
} And what about the requirements for trucking?
} If it's going to spend two or three hours on the
} interstate, will any sort of truck do the job?
}
} - John
}
}
}
}



From daemon Fri Oct 26 08:13:34 2001



From: David Wilbur :      dwilbu01-at-emerald.tufts.edu
Date: Fri, 26 Oct 2001 08:47:54 -0400
Subject: Re: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My experience is only anecdotal, but highly successful.

About a month ago I moved a JEOL-840 about 80 miles. The scope was
donated
to the University by a large corporation. It was decommissioned by a
JEOL
engineer, but it did not appear to have any special preparation other
than
to have the spring loaded column table bolted down. It was put on a
pallet
and moved to the shipping department by professional movers. From there
on
it was strictly an amateur job. I rented a Ryder truck, which I drove
myself. Be sure the truck is high enough to do the job. I had to
remove an
ion pump at the top of the column in order to get the scope through the
door
of the truck. I unloaded it on my end with the help of one other person
and
a hydraulic pallet mover. There was a power lift available on both ends
to
match the height of the truck bed to the height of the loading dock.
Two
people (middle aged and not particularly hefty) were able to get the
scope
off the pallet and to its final position, using pry bars and 1/2 in
aluminum
rods as rollers. I did the installation myself, and it is now working
much
better than the similar but older scope it replaced.

If you have a few strong, but also smart helpers and some modest
equipment,
there is no need to fear the job. Work slowly and carefully. Feel free
to
call me if you want further details.

Dave Wilbur



--
__________________________________
David J. Wilbur, Ph.D.
Instrumentation Specialist
Department of Chemistry
Tufts University
voice: 617-627-2163
Fax: 617-627-3443
email: dwilbu01-at-tufts.edu
__________________________________




From daemon Fri Oct 26 08:25:10 2001



From: Dave Hall :      davehall-at-restechimage.com
Date: Fri, 26 Oct 2001 09:22:12 -0400
Subject: Nikon Coolscan 8000ED

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Paul B. Grover" {pbgrover-at-home.com}
To: "John Foust" {jfoust-at-threedee.com} ; {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 25, 2001 7:30 PM


Rick:

I second the notion that the AGFA T2500 Duoscan is and excellent scanner.
My understanding is that the significant internals are made by Microtek
which makes the AGFA T2500 nearly identical to the Microtek ArtixScan 2500
(another excellent scanner.) We also have customers quite pleased with
these "twin" units. It's also worth noting, from what I've been told, that
AGFA is discontinuing its T2500. I don't know if it's out of production
yet, but I do know that because of the discontinuation, we've been able to
offer good discounting to the customers we've proposed it to. If you choose
to pursue the AGFA, (some inventory is still available) your dealer should
be able to offer the same.

Good Luck.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045
*Microscopy and Digital Image Analysis for Science and Industry



-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Thursday, October 25, 2001 3:49 PM
To: r.webb-at-mailbox.uq.edu.au; Microscopy-at-sparc5.microscopy.com


Rick,
Many of our customers are very happy with the 8000ED.
I have looked into putting TEM negs into it and it could be
done by modifying one of the 120/220 film holders.
The holders have a raised lip to keep the film in the
channel. I believe these could be removed and the film could then
just extend out past the scan opening. I have not been able to try
this as demand for the scanner has been very high.
Another excellent scanner for TEM negs is the Agfa
T2500 Duoscan. While lower in resolution(2500dpi optical) it has
a glassless carrier design that will enable scanning of an entire
TEM negative. It also will scan reflective originals.

George

George Laing
National Graphic Supply
v:(800) 223-7130 x3109
f:(800) 832-2205
email: scisales-at-ngscorp.com

-----Original Message-----
} From: Rick Webb [mailto:r.webb-at-mailbox.uq.edu.au]
Sent: Wednesday, October 24, 2001 6:01 PM
To: Microscopy Listserver


Hi

I have just seen the new Nikon Super Coolscan 8000ED demonstrated. It
was certainly very impressive. The 4000dpi and the density range of 4.2
make it a great scanner for EM negatives. I'm at present using a
LeafScan 45 and this new Nikon was equal to or better than it in all
areas and at a fraction of the cost. I have only a couple of
reservations about it. The only negative holders that we saw
demonstrated would not hold a standard (3 1/4 x 4 in , 8.8 x 10.2cm) TEM
negative. We were forced to cut down the negatives. Has anyone been able
to construct an adapter to take a full negative? I'm not too worried
about the fact that it won't scan the entire area of the negative but
don't like the idea of having to cut up negatives. It also seemed that
the construction of the negative carriers did not appear to be very
solid. I worry that in our multi user centre they may not last very
long.

I'd be keen to hear from anyone who has experience with this scanner

Thanks

Rick
--

===================================================
Rick Webb
Senior Research Officer
Department of Microbiology and Parasitology and
Centre for Microscopy and Microanalysis
University of Queensland 4072
Australia

Ph (+61)- 7-3365-1138
Fax (+61)- 7-3365-2199
==================================================





From daemon Fri Oct 26 09:25:40 2001



From: Christine Broadbridge :      broadbridge-at-southernct.edu
Date: Fri, 26 Oct 2001 10:19:23 -0400
Subject: Service providers for a Cambridge SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

We would appreciate any information/suggestions concerning service
providers for a recently acquired Cambridge Stereoscan 240 SEM. To start we
would be interested in a routine inspection and maintenance visit. We are
located in Southern Connecticut.
Thanks very much!




*************************************
Christine Caragianis Broadbridge, Ph. D.
Associate Professor of Physics
Southern Connecticut State University
501 Crescent Street; Jennings 115
New Haven, CT 06515-1355

Office: 203-392-6461
Lab: 203-392-7018
Fax: 203-392-6466

broadbridge-at-southernct.edu




From daemon Fri Oct 26 10:25:32 2001



From: rnessler :      rnessler-at-blue.weeg.uiowa.edu
Date: Fri, 26 Oct 2001 10:18:35 -0500
Subject: Balzers 301 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello list,
It's been awhile since I needed work done on our Balzers 301 freeze
fracture unit, so I'm asking who provides service on these instruments now?
Randy Nessler
University of Iowa
Phone 319-335-8142



From daemon Fri Oct 26 10:50:44 2001



From: woxberry-at-downstate.edu
Date: Fri, 26 Oct 2001 10:43:51 -0500
Subject: core lab managers-what info do I need?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers-

Can anyone give me their thoughts on the design of a request form for
users of a
microscopy core facility. I have done clinical TEM for twenty years but now
I've been asked to manage a confocal/TEM core and will be getting a
more diverse
mix of specimens. What info should I request from the users?
The obvious:1)project design- what questions do you wish to answer
2)sample type- cells, tissue etc.
3) fixed or live
4) fixation method
5) probes?
6) embedding method
7) etc. ?????

should there be two separate forms- one for TEM and one for Confocal? or one
general form to include both microscopies?

What do you do? Maybe someone can fax me a copy of their labs
request form that
I can use as a template.
Thank you all.
Sincerely,
Bill Oxberry
Health Science Center Brooklyn
fax # 718-270-3313


From daemon Fri Oct 26 11:42:59 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Fri, 26 Oct 2001 12:54:22 -0400
Subject: RE: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John:
It can be done...I managed with the able assistance of a former student to
shift a Hitachi 570 LB via a rental truck from the Smithsonian Institute in
Washington, D.C. to Binghamton, NY. `
Success is dependent largely on the care taken with dismantling and
packing. If possible, consult a service engineer or a firm that services
SEMs of that manufacturer. They should be able to give advice on
precautions to take prior to dismantling and shipment. Check out the SEM
manual. It should give you exact dimensions and weights for each main part.
The height can turn out to be all important...but more of that later.
Plan on using lots of labeling tape to label connections. Stretch wrap
which comes in 5" and 20" width rolls comes in useful. Boxed components
should be plastic or stretch wrapped and well padded.
There will be water hoses that will have to be disconnected and
drained...and perhaps a water chiller to drain. Disconnect the vacuum hose
and pack the rotary pump and make sure its kept upright. Disconnect
connections between the display and column units.
The cable connecting to the gun and high voltage tank should be removed and
the ends stretch wrapped. Pack the cables with care to avoid bending too
sharply. As with car spark plug leads the insulation could be damaged.
Usually the column is suspended on anti-vibration pads. There should be
bolts that can be applied to make the column firm for shipment. Ideally the
column should shipped under vacuum. If you remove the top gun section, use
a blanking plate to seal the rest of the column and rough pump before
shipping.
For strapping to pallets and to secure to truck interior you will find
belt straps and plastic poly strapping come in handy. Strong backs help,
but I would advise having the use of a "J" bar and a heavy duty pallet
truck.
And speaking of pallets... Just as we were on the loading dock and moving
the column unit into the truck we discovered to our consternation we were
just over one inch too tall to fit through the truck's door. So don't
forget to figure in the height of the column PLUS the pallet when selecting
a truck with a tall enough loading door. For us, this meant having to break
the SEM column vacuum and remove the gun segment which housed the LB6
filament and ion pump, which fortunately was sealed off under it own vacuum.
We covered the lower column with a metal plate and had to ship it without
being under vacuum.
Upon arrival back in Binghamton, NY the SEM had to be temporally stored in
a hallway without access to a 208 V,30 AMP power supply. I had to rig up a
system to keep the lower column under rough vacuum until the SEM could be
properly installed in its permanent position. I had hole drilled into a
half-inch thick aluminum plate cover and threaded to fit a pipe with a gas
valve shut-off and a barbed end to fit a vacuum hose. This allowed me to
rough-pump the column from the top.
It was an interesting experience not to mention the coming and going to the
Smithsonian Institute during a rather lively time when streets were blocked
off during the World Trade Organization protests...but that's another story.
Good luck with your move,
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

John Foust wrote:
} I'm looking for advice or anecdotes about moving
} heavy SEM equipment. I'm hoping to take possession
} of an old SEM, whose column base is 33"x33" and may
} weigh as much as 1,200 pounds. The console is
} wider and may weigh only 300 pounds.
}
} If I could avoid $1,000 on professional movers and
} wrestle it with a few strong guys, that would be great,
} but there are other questions in my mind.
}
} Regardless of who moves it, what considerations must
} they take regarding the sensitive nature of the
} equipment? Certainly sensitive bits like filaments
} should be removed and handled separately, right?
}
} And what about the requirements for trucking?
} If it's going to spend two or three hours on the
} interstate, will any sort of truck do the job?
}
} - John

--
************************************************
Philosophy is the microscope of thought.

Victor Hugo, Book 3 Chapter 3 Les Miserables
************************************************
Laura Rhoads, Assistant Professor
Biology Department
SUNY Potsdam
44 Pierrepont Avenue
Potsdam, NY 13676
315-267-2260
315-267-3170 fax



From daemon Fri Oct 26 11:44:20 2001



From: David Cherns :      d.cherns-at-bris.ac.uk
Date: Fri, 26 Oct 2001 17:23:27 +0100
Subject: TEM Postdoctoral position in electron holography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A postdoctoral position for electron holography studies of GaN structures is
available for 14 months from 1 December 2001.

The project makes use of a Hitachi HF2000 FEGTEM equipped with an electron
biprism and Gatan Imaging Filter and aims to develop methods of profiling
electric fields which exist at interfaces and around defects. A good
background in TEM is essential. Experience in electron holography is not
essential.

For further details, contact Prof D. Cherns by e-mail, fax or phone as below

David Cherns
H.H. Wills Physics Laboratory
University of Bristol
Tyndall Avenue
Bristol BS8 1TL

Tel +44 (0)117 9288702
Fax +44 (0)117 9255624
E-mail D.Cherns-at-bris.ac.uk



From daemon Fri Oct 26 12:10:19 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 26 Oct 2001 13:04:45 -0400
Subject: TEM of semiconducting materials: the Multiprep system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's not too late!

If you want better semiconductor and GaN on sapphire samples than you can ever get by polishing and ion milling, may I suggest that you look into using the MicroCleave technique (also known as the small angle cleavage technique). I would recommend that you visit the South Bay Technology web site and check out the PDF files that they have on the kit. You will see an example of GaN on sapphire in one of their PDF files. There is no ion milling damage present at all. That was one of about five samples that was prepared in about two hours with the kit. Their web site is
www.southbaytech.com.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Massimo Catalano [mailto:massimo.catalano-at-ime.le.cnr.it]
Sent: Friday, October 26, 2001 2:53 AM
To: Microscopy-at-sparc5.microscopy.com


Dear colleagues,

our group works on Transmission Electron Microscopy of semiconducting
materials, especially low-dimensional materials for optoelectronic
applications. We work on InGaAs/GaAs single and stacked quantum dots and we
recently started a new research activity on GaN on sapphire and/or Alumina
substrates.

We have a good experience with TEM sample preparation, but we recently
decided to buy the Multiprep System, to exploit new methods for TEM sample
preparation and to minimize the ion milling time.

We just assembled and calibrated the machine, and we are now in the
progress of preparing the first plan-view and cross-sectional samples.

As our machine is one of the few presently available in Italy, we are in
contact with Allied to have suggestions and directions.

I should appreciate it very much if those of you who have operated the
machine can give us suggestions, hints and procedures to get good quality
samples with this nice machine.

Thanks in advance for your help

Massimo

Dr. Massimo Catalano
IME-CNR
Via Arnesano
73100 Lecce - ITALY
phone: +39 0832 391199
fax: +39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it



From daemon Fri Oct 26 13:02:06 2001



From: Szostak, Frank :      szostakf-at-aecl.ca
Date: Fri, 26 Oct 2001 13:54:19 -0400
Subject: Job posting at AECL Chalk River Labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






} The Surface Science Laboratory at AECL Chalk River Labs has an opening for
} a professional surface scientist. Anyone interested in applying is
} encouraged to contact either Bill Hocking or Ian Muir. This position will
} soon appear on the AECL website (www.aecl.ca) under Careers. Please use
} this opportunity to apply on-line.
}
} Ian Bill
} ----------------------------------------------
} -----------------------------------------------
} Ian J. Muir William H. Hocking, Ph.D.
} Corrosion and Surface Science Branch Section Head, Surface Science
} AECL, Chalk River Laboratories Corrosion and Surface Science Branch
} Chalk River, Ontario AECL, Chalk River Laboratories
} Canada K0J 1J0 Chalk River, Ontario, Canada
} ph: (613) 584-8811 ext. 6960 K0J 1J0
} fax: (613) 584-3250 ph: (613) 584-8811 ext. 4651
} email: muiri-at-aecl.ca {mailto:muiri-at-aecl.ca} fax: (613) 584-3250
} email: hockingw-at-aecl.ca {mailto:hockingw-at-aecl.ca}
}
}
}
} Surface Science Laboratory
}
} The Surface Science Laboratory is part of the Corrosion and Surface
} Science Branch within the Fuel Channels Division at AECL. The Laboratory
} is equipped with a VG Scientific ESCALAB 220i-XL Imaging X-ray
} photoelectron Spectrometer (XPS), a CAMECA ims 6F secondary ion
} microanalyzer (SIMS), a Physical Electronics SAM 670 scanning Auger
} microprobe and two JEOL scanning electron microscopes (SEM). An SEM/EDX
} system is available for analysis of low activity samples, whereas highly
} radioactive materials are accommodated in a shielded SEM/WDX facility.
} The XPS and SIMS instruments are located in modern laboratories designed
} for studies of highly radioactive samples and both systems have been
} configured for safe handling of such samples. Activities in the Surface
} Science Laboratory range from fundamental studies on nuclear materials to
} commercial projects and failure analyses with a focus on characterization
} of radioactive materials.
}
} Surface Science Position
}
} A research scientist is required to conduct studies of diverse materials
} degradation and aging problems in the nuclear industry using secondary ion
} mass spectrometry (SIMS) and other surface science methods. Applications
} will be focused primarily on radioactive samples taken from reactor
} components.
}
} Duties:
} * Stress-corrosion cracking (SCC) of feeder pipes and steam-generator
} tubes will be the principal area of initial focus, although support for
} on-going studies of corrosion and deuterium ingress in pressure tubes will
} be important as well.
} * Development of expertise in other areas of applied surface science,
} through participation in projects that require a multi-technique approach,
} will also be expected. This will involve using scanning Auger microscopy
} (SAM), imaging X-ray photoelectron spectroscopy (XPS) and scanning
} electron microscopy (SEM) with energy dispersive X-ray (EDX)
} microanalysis.
} * Preparation of proposals for commercial and internal work, and
} reporting to customers on such projects, will be key professional
} activities.
} *
} * Qualifications:
} * A graduate degree in surface science and a working knowledge of the
} practical application of modern surface analytical methods, especially
} SIMS, to industrial problems are required.
} * Any additional training in, or experience with, electronics,
} computers, spectroscopy, metallography, corrosion processes, radioactive
} materials, or ultra-high vacuum technology would be an asset.
}
} The candidate must be a highly motivated individual with good written and
} oral communications skills, who will be able to exploit the commercial
} potential of the active surface science capabilities at CRL.
}


From daemon Fri Oct 26 13:30:45 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 26 Oct 2001 14:22:42 -0400
Subject: Choose an Ultracut?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I would appreciate some practical help in choosing between the Leica
(Reichert) Ultracut R and the Ultracut S on merit alone, notwithstanding
considerations of price.

Thanks,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Fri Oct 26 13:40:07 2001



From: Eric Steel :      eric.steel-at-nist.gov
Date: Fri, 26 Oct 2001 14:34:29 -0400
Subject: Post Doctoral Positions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The National Institute of Standards & Technology (NIST) has many Post
Doctoral Positions open. These are offered competitively through the
National Research Council (NRC). Within microscopy &
microanalysis research areas we have many possible openings described at
the web site listed below. The Microanalysis Research Groups at NIST have
about twenty full time scientists specializing in the measurement methods
in our extensive facilities including SEMs (FEG, EPMA, Environmental,…),
AEMs (FEG/LaB6 TEM/STEM/EDS/EELS), Auger probes, SIMS/TOF-SIMS,
MicroXRD/XRF, MicroRaman/IR, Synchrotron beam-line with grazing incidence
XPS, etc.

We research new and improved microscopy and microanalysis measurement
methods and we apply these methods to challenging analytical problems in
semiconductor and optoelectronic technology, materials science,
environmental and earth science, etc.

The NIST/NRC Post Doc program offers a two-year position at an annual
salary of approximately $53,200 with an additional $5,500 for research
expenses. The applications are due to the NRC by Jan 15, 2002. This must
include a brief proposal and several recommendations.

A candidate must be a U.S. citizen and start work (with their PhD in hand)
at NIST between July 2002 and January 2003. So, this is the perfect
opportunity for those that are graduating this spring through next fall and
others that have received their degree within the last five years. (Please
note that NIST/NRC only competes Post Doc positions once a year, unlike
some other institutions. )

Please contact me or go to the following site for further info:

http://www.cstl.nist.gov/div837/Division/opportunities/PostDoctoral.htm



From daemon Fri Oct 26 16:45:28 2001



From: The Working Boy :      mark.grimson-at-TTU.EDU
Date: Fri, 26 Oct 2001 15:37:33 -0600
Subject: Student microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are in the process of ordering 30 student compound light microscopes for
an upper level lab course. Our lowest bid ($400.00 below the next lowest)
was for Wesco (or Para/Wesco) microscopes. Has anybody had any expierience
with Wesco student scopes and can you make any recommendations. Thank you.

Mark J. Grimson

IMPORTANT NOTE:
As required by a state of Texas mandate concerning formatting of
state employee e-mail addresses, my new e-mail address is:
mark.grimson-at-ttu.edu. The old one should continue to work for quite
awhile, but you may want to go ahead and make this change in your
address book. Thank you.

Mark J. Grimson, Ph.D
Electron Microscopy Facility
Dept. of Biology, MS 3131
Texas Tech University
Lubbock, Texas 79409

(806)742-2704 (phone)
(806)742-2963 (Fax)
mark.grimson-at-ttu.edu




From daemon Fri Oct 26 22:11:38 2001



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Sat, 27 Oct 2001 16:04:58 +0900
Subject: Pressure sensitive film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the best student microscopes are for Meopta ( or Lambda) Czech rep.
address is available on www.coleoptera.org in section scientific suppliers.

They have very good optical resolution, and very good design

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).



----- Original Message -----
} From: "The Working Boy" {mark.grimson-at-TTU.EDU}
To: "MSA" {microscopy-at-sparc5.microscopy.com}
Sent: Saturday, October 27, 2001 7:37 AM


Dear all:

I know that somebody asked a question on how to observe paper structure. This is a little different case. I want to observe the structure of pressure sensitive film, Pressurex Film from Fuji. I am not aware enough of the film structure, but I wonder if I could see the ink capsules which normally breaks and provides red inks on being pressurized.
If anybody could explain the film structure and/or the sample prep method, I would appreciate it. I am worried that the organic capsule would break and make mess under the e beam in SEM. If I make the film dry completely, would I see the capsule original spherical or like shrunk baloon?

Best regards,

Jondo Yun
jdyun-at-kyungnam.ac.kr


From daemon Sat Oct 27 08:10:14 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 27 Oct 2001 09:04:31 -0400
Subject: Re: Pressure sensitive film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jondo Yun,
Many years ago I had a customer who routinely checked NCR paper for
quality control, checking the size and distribution of the ink balls. I
believe he just mounted and coated the samples. There was no problem
with bursting spheres, etc.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Jondo Yun À±Á¸µµ wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all:
}
} I know that somebody asked a question on how to observe paper structure. This is a little different case. I want to observe the structure of pressure sensitive film, Pressurex Film from Fuji. I am not aware enough of the film structure, but I wonder if I could see the ink capsules which normally breaks and provides red inks on being pressurized.
} If anybody could explain the film structure and/or the sample prep method, I would appreciate it. I am worried that the organic capsule would break and make mess under the e beam in SEM. If I make the film dry completely, would I see the capsule original spherical or like shrunk baloon?
}
} Best regards,
}
} Jondo Yun
} jdyun-at-kyungnam.ac.kr
}
}
}



From daemon Sun Oct 28 19:36:39 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 29 Oct 2001 14:14:28 GMT+1200
Subject: Coating the bell-jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again

I never used to have any problem, every so often I just sprayed with
a multi-solvent household cleaner and the carbon coating on the
inside of the bell-jar wiped off easily.

Now I'm using a different bell-jar, one that had been used for Al and
that I had to clean up with acids, and I can't budge the carbon
resulting from a few useages. I guess that the acid treatment somehow
activated the glass surface so that the carbon really stuck.

Two questions:

How can I clean off this stubborn coating?

How can I prevent it from happening in the future?


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Sun Oct 28 23:05:15 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 29 Oct 2001 01:55:42 -0500
Subject: Vacuum bell jar clean up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ritchie Sims wrote:
=======================================================
I never used to have any problem, every so often I just sprayed with a
multi-solvent household cleaner and the carbon coating on the inside of the
bell-jar wiped off easily.

Now I'm using a different bell-jar, one that had been used for Al and that
I had to clean up with acids, and I can't budge the carbon resulting from a
few useages. I guess that the acid treatment somehow activated the glass
surface so that the carbon really stuck.

Two questions:

How can I clean off this stubborn coating?

How can I prevent it from happening in the future?
========================================================
So far as removing what is already there, probably you are going to have to
use some amount of good old-fashioned rubbing and scrubbing, using acetone
and lint free cotton wipers. You can't do anything about the past but you
can do something about the future.

And that is to use Bell Bright™ Bell Jar Spray, as shown on URL
http://www.2spi.com/catalog/supp/supp3.html

A very thin layer of a water soluble polymer is left behind on the glass
surface so that when it is time for a cleaning, a good lint-free cotton
wiper and water alone, will cause the deposit to lift right off. In
addition to saving a lot of hard work, you eliminate the breathing of
acetone vapors.

Now for three words of further comment:

1] The formulation is not substantially different from a typical cosmetic
hair spray, however it does not have the various additives that would
otherwise gunk up a vacuum system.

2] It is a "gas under pressure" which means it is "dangerous goods" from an
international shipment point of view, so it is expensive to ship by itself.
It can be shipped only by air freight.

3] Disclaimer: SPI Supplies has formulated and sold this product since
about 1978, so we have a vested interest in seeing more of it being consumed

From daemon Mon Oct 29 01:40:40 2001



From: Rene Rodrigez :      rene-at-proj.com
Date: Sun, 28 Oct 2001 22:00:58 -0800
Subject: Instructors: Announcing New Server Side Train the Trainer workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I found your name on the website as someone who teaches existing server-side Java courses. My company has developed a new, practical server-side Java course, and we would like you to consider offering it to your students in the future.

You can view the syllabus of this new class that we developed at www.basebeans.com . The web site has the complete details of the Train the Trainer workshop. As technology advances from servlets to JSPs, the next step is towards JSP frameworks. As an instructor, I'm sure you are aware of the constant need to stay ahead of the curve.

If you would contact me at (415) 781-1463, I can tell you more.
Thanks,
Rene


Microscopy-at-MSA.Microscopy.Com



From daemon Mon Oct 29 08:28:27 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 30 Oct 2001 00:16:41 +1000
Subject: RE: Coating the bell-jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1 Jiffy or another fine household abrasive.
2 Coat the inside of the belljar with dilute (roughly 1:20) dishwashing
detergent. Dry before use.
Less carbon will bounce off the coated belljar. So it gets dark faster, but it
just about floats off with a bit of hot water.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, October 30, 2001 12:14 AM, Ritchie Sims
[SMTP:r.sims-at-auckland.ac.nz] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi again
}
} I never used to have any problem, every so often I just sprayed with
} a multi-solvent household cleaner and the carbon coating on the
} inside of the bell-jar wiped off easily.
}
} Now I'm using a different bell-jar, one that had been used for Al and
} that I had to clean up with acids, and I can't budge the carbon
} resulting from a few useages. I guess that the acid treatment somehow
} activated the glass surface so that the carbon really stuck.
}
} Two questions:
}
} How can I clean off this stubborn coating?
}
} How can I prevent it from happening in the future?
}
}
} cheers
}
} rtch
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand


From daemon Mon Oct 29 08:51:01 2001



From: DrJohnRuss-at-aol.com
Date: Mon, 29 Oct 2001 09:45:36 EST
Subject: Image Processing Toolkit Upgrade

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Version 4.0 of the Image Processing Tool Kit is now available. Free update
copies have been sent to all registered purchasers of version 3.0, so if you
neglected to send in the original registration card, now would be a good time
to do it!
New features, etc., are described at http://ReindeerGraphics.com


From daemon Mon Oct 29 10:17:31 2001



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Tue, 30 Oct 2001 10:52:18 -0500
Subject: Rebuild PGT detector

Contents Retrieved from Microscopy Listserver Archives
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Listers,

Am looking for a source to rebuild PGT detector. Crystal and/or the FET
needs redone. I know PGT does things different than any of the other guys as
far as the detector. Any names would be helpful. Thanks.

Joel McClintock
EM Specialist
U of Kentucky
859-257-1242



From daemon Mon Oct 29 10:19:09 2001



From: William Jany :      bill-at-clarkemosquito.com
Date: Mon, 29 Oct 2001 10:05:43 -0600
Subject: Microscope Repair - Need contacts

Contents Retrieved from Microscopy Listserver Archives
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My company has a number of standard light microscopes which are used by our
traveling technicians.

They are taking a terrible beating.

Is there anybody on the list near Roselle, IL which we can contract with for
regular maintanance, repair, upkeep ect.


Also can anyone recommend a microscope that could survive this type of
constant wear better than others?


Thank you for your time

Bill Jany
Clarke Mosquito Control
110 E Irving Park Road, 4th floor
Roselle, IL 60173
630-671-3121
FAX: 630-894-1171


From daemon Mon Oct 29 11:33:48 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 29 Oct 2001 12:11:24 -0500
Subject: Coating the bell-jar

Contents Retrieved from Microscopy Listserver Archives
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I once cleaned a very stubborn coating on a glass bell jar by using aluminum foil. Just crumple it up and start wiping. It takes time, but it will come off. I recommend that you use a product like bell bright on the glass prior to using the coater. It puts a layer between the glass and the deposit that is easily to take off with a wet cloth.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Monday, October 29, 2001 9:14 AM
To: Microscopy-at-sparc5.microscopy.com


Hi again

I never used to have any problem, every so often I just sprayed with
a multi-solvent household cleaner and the carbon coating on the
inside of the bell-jar wiped off easily.

Now I'm using a different bell-jar, one that had been used for Al and
that I had to clean up with acids, and I can't budge the carbon
resulting from a few useages. I guess that the acid treatment somehow
activated the glass surface so that the carbon really stuck.

Two questions:

How can I clean off this stubborn coating?

How can I prevent it from happening in the future?


cheers

rtch

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Oct 29 11:33:48 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Mon, 29 Oct 2001 12:25:41 -0500
Subject: RE: Balzers 301 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reps for Bal-Tec in USA:

Technotrade International 7 Perimeter Road Manchester, NH 03103-3343 USA Mr. A. Auwärter (GM) / Mr. J. Hagen (Sales) Mr. C. Tavernini ( Sales) T: +1 603 622 50 11 F: +1 603 622 52 11 E-mail: albie-at-technotradeinc.com E-mail: johnny-at-technotradeinc.com E-mail: carlo-at-technotradeinc.com


Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


} Randy Nessler
} University of Iowa
} Phone 319-335-8142
}
}
}


From daemon Mon Oct 29 16:28:48 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 30 Oct 2001 11:16:26 GMT+1200
Subject: Re: Rebuild PGT detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Listers,
}
} Am looking for a source to rebuild PGT detector. Crystal and/or the
} FET needs redone. I know PGT does things different than any of the
} other guys as far as the detector. Any names would be helpful.
} Thanks.
}


Hi, Joel

I'm fairly sure that Gresham, of the UK, will rebuild any brand of
detector. I think that Jim Nicolino, of X-Ray Optics, Florida, is
their US representative.

But why not involve PGT?

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Oct 29 17:13:04 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Mon, 29 Oct 2001 18:07:18 -0500 (EST)
Subject: need sem protocol

Contents Retrieved from Microscopy Listserver Archives
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Hi listers,

I've been asked to look at some polystyrene microspherules in the SEM and
would like some advice on how to prepare them. I'm not a biomedical
person, so need some help!

The spherules are 5.6 um size with a Lumidin (protein) bonding surface and
receptor molecules attached. They're in DI (or a DI solution?), which is
apparently the delivery system for the marker molecules. Would they need
CPD? If so, I've never CPD'd particles in solution before.... The SEM has
hivac and VP modes, plus there's a cool stage.

Any ideas would be highly appreciated!
Many thanks,
Dee




***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Mon Oct 29 17:38:27 2001



From: Arrowood, Roy :      arrowood-at-utep.edu
Date: Mon, 29 Oct 2001 16:31:11 -0700
Subject: another question about the bell jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


(Dr. Ritchie Sims on "Coating the Bell Jar" asked how to remove stubborn
carbon deposits in a bell jar used to coat aluminum, then acid-etched, then
reused with carbon.)

In making carbon fibers for composite materials, it is fairly common for
manufacturers to activate the carbon surface (so that it will bond to matrix
polymer or to a coupling agent) with an oxidizing-acid etch (often sulfuric
or nitric). Dr. Sims, what acid(s) did you use, and at what concentration?
Was the acid treatment applied again after carbon deposition, or only
before?
-------Roy

====================================
Roy Arrowood, Associate Professor
Metallurgical and Materials Engineering
UTEP, El Paso, TX 79968-0520
(915)747-6934
NEW E-MAIL ADDRESS: arrowood-at-utep.edu



From daemon Tue Oct 30 08:23:13 2001



From: Randall, Kevin J :      Kevin.Randall-at-astrazeneca.com
Date: Tue, 30 Oct 2001 13:59:35 -0000
Subject: Antibody to SDAV

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I have been asked to demonstrate, by immunohistochemistry, the presence of
sialodacryoadenitis virus, a rat coronavirus, in formalin fixed paraffin
embedded tissue. Does anyone know of a commercially available antibody
against SDAV that works in paraffin?

Thanks in advance

Kevin



From daemon Tue Oct 30 09:05:51 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 30 Oct 2001 08:57:36 -0600
Subject: Re: need sem protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dee,

Unless they are microballoons, I wouldn't think you would need to do much
special. I would probably start quick and dirty and get more complicated if
necessary. I would try laying some down on a substrate and try crushing a
few between glass slides to get a look at the interior to make sure the
spheres are solid.

I would gold coat and use high vacuum mode in order to see much detail. I
don't know what your clients want to know. We have some here trying to make
small spheres and they are usually somewhat interested in the surface so we
have to push for all the resolution we can get.

Warren

At 06:07 PM 10/29/01 -0500, you wrote:

} Hi listers,
}
} I've been asked to look at some polystyrene microspherules in the SEM and
} would like some advice on how to prepare them. I'm not a biomedical
} person, so need some help!
}
} The spherules are 5.6 um size with a Lumidin (protein) bonding surface and
} receptor molecules attached. They're in DI (or a DI solution?), which is
} apparently the delivery system for the marker molecules. Would they need
} CPD? If so, I've never CPD'd particles in solution before.... The SEM has
} hivac and VP modes, plus there's a cool stage.
}
} Any ideas would be highly appreciated!
} Many thanks,
} Dee
}
} ***************************************************************
} Please do not publicly post any of my correspondence without permission
}
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 845/365-8640

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Tue Oct 30 09:21:26 2001



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Tue, 30 Oct 2001 10:34:42 -0500
Subject: RE: Moving SEM equipment

Contents Retrieved from Microscopy Listserver Archives
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Peter:
I've had to replace a number of parts recently for our Hitachi S 570 LB.
All of the items were shipped promptly by Hitachi Instruments Inc. (800)
253-3053. In the future when parts may no longer be available from the
manufacturer, I would try asking for help right here on the MSA list serve.
Hopefully, someone will be able to come to the rescue.
Henry

-----Original Message-----
} From: Peter Tomic [mailto:PTomic-at-Anadigics.com]
Sent: Monday, October 29, 2001 8:50 AM
To: 'Henry Eichelberger'


John:
It can be done...I managed with the able assistance of a former
student to
shift a Hitachi 570 LB via a rental truck from the Smithsonian Institute in
Washington, D.C. to Binghamton, NY. `
Success is dependent largely on the care taken with dismantling and
packing. If possible, consult a service engineer or a firm that services
SEMs of that manufacturer. They should be able to give advice on
precautions to take prior to dismantling and shipment. Check out the SEM
manual. It should give you exact dimensions and weights for each main part.
The height can turn out to be all important...but more of that later.
Plan on using lots of labeling tape to label connections. Stretch
wrap
which comes in 5" and 20" width rolls comes in useful. Boxed components
should be plastic or stretch wrapped and well padded.
There will be water hoses that will have to be disconnected and
drained...and perhaps a water chiller to drain. Disconnect the vacuum hose
and pack the rotary pump and make sure its kept upright. Disconnect
connections between the display and column units.
The cable connecting to the gun and high voltage tank should be
removed and
the ends stretch wrapped. Pack the cables with care to avoid bending too
sharply. As with car spark plug leads the insulation could be damaged.
Usually the column is suspended on anti-vibration pads. There
should be
bolts that can be applied to make the column firm for shipment. Ideally the
column should shipped under vacuum. If you remove the top gun section, use
a blanking plate to seal the rest of the column and rough pump before
shipping.
For strapping to pallets and to secure to truck interior you will find
belt straps and plastic poly strapping come in handy. Strong backs help,
but I would advise having the use of a "J" bar and a heavy duty pallet
truck.
And speaking of pallets... Just as we were on the loading dock and
moving
the column unit into the truck we discovered to our consternation we were
just over one inch too tall to fit through the truck's door. So don't
forget to figure in the height of the column PLUS the pallet when selecting
a truck with a tall enough loading door. For us, this meant having to break
the SEM column vacuum and remove the gun segment which housed the LB6
filament and ion pump, which fortunately was sealed off under it own vacuum.
We covered the lower column with a metal plate and had to ship it without
being under vacuum.
Upon arrival back in Binghamton, NY the SEM had to be temporally
stored in
a hallway without access to a 208 V,30 AMP power supply. I had to rig up a
system to keep the lower column under rough vacuum until the SEM could be
properly installed in its permanent position. I had hole drilled into a
half-inch thick aluminum plate cover and threaded to fit a pipe with a gas
valve shut-off and a barbed end to fit a vacuum hose. This allowed me to
rough-pump the column from the top.
It was an interesting experience not to mention the coming and going
to the
Smithsonian Institute during a rather lively time when streets were blocked
off during the World Trade Organization protests...but that's another story.
Good luck with your move,
Henry

Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu

John Foust wrote:
} I'm looking for advice or anecdotes about moving
} heavy SEM equipment. I'm hoping to take possession
} of an old SEM, whose column base is 33"x33" and may
} weigh as much as 1,200 pounds. The console is
} wider and may weigh only 300 pounds.
}
} If I could avoid $1,000 on professional movers and
} wrestle it with a few strong guys, that would be great,
} but there are other questions in my mind.
}
} Regardless of who moves it, what considerations must
} they take regarding the sensitive nature of the
} equipment? Certainly sensitive bits like filaments
} should be removed and handled separately, right?
}
} And what about the requirements for trucking?
} If it's going to spend two or three hours on the
} interstate, will any sort of truck do the job?
}
} - John

--
************************************************
Philosophy is the microscope of thought.

Victor Hugo, Book 3 Chapter 3 Les Miserables
************************************************
Laura Rhoads, Assistant Professor
Biology Department
SUNY Potsdam
44 Pierrepont Avenue
Potsdam, NY 13676
315-267-2260
315-267-3170 fax



From daemon Tue Oct 30 09:53:17 2001



From: Judy Zhu :      jzhu-at-multiplexinc.com
Date: Tue, 30 Oct 2001 08:18:59 -0800
Subject: SEM sample prep: stain-etch recipe for InP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings-

I have an InP p-n junction device with Zn-diffused p-region. I would like to
stain-etch the cross-section to observe the junction with SEM. Does anyone
have a recipe for this stain-etch? Thanks.

Regards,

Judy Zhu, Ph.D.
Member of Technical Staff
Multiplex, Inc.
115 Corporate Blvd.
South Plainfield, NJ 07080
phone: 908-757-8817 x 1167
fax: 908-757-8910
email: jzhu-at-multiplexinc.com




From daemon Tue Oct 30 10:45:50 2001



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Tue, 30 Oct 2001 11:45:19 -0500
Subject: Antibody against perforin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear netters,

I've been attempting to do immunogold labelling of 4% paraformaldehyde
fixed human natural killer cells (pre- and post- embedding). We're
trying to label against perforin, but haven't had success. A monoclonal
antibody we have labels the protein in cells fixed with methanol, but we
need to preserve the ultrastructure of the cells. Is anyone using an
antibody against the protein that works well in paraformaldehyde fixed
cells? If so, would you please tell me what vendor you purchased it
from, and perhaps share your protocol? Thank you.

Edward Haller
University of South Florida
Pathology Department
Tampa, Florida(813)974-9584


From daemon Tue Oct 30 10:45:52 2001



From: William P. Sharp :      wsharp-at-asu.edu
Date: Tue, 30 Oct 2001 09:37:52 -0700
Subject: "New" Student Microscopist

Contents Retrieved from Microscopy Listserver Archives
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Listers -

I have become aware of a Community College student, Wil Kunkel, at
Scottsdale Community College who will be joining this list in the near
future. Wil takes some courses at AZ State U as well, and ended up on my
door step with some questions re: SEMs.

It seems that SCC was the recipient of an ISI SX40 scanner from a local
business which retired the instrument when its principal operator retired.
Neither Wil nor the professor who was the receiver of the gift have any
background in microscopy, but Dr. Sickafouse turned Wil loose on the scope
after it was dumped in the room and somehow, Wil put it together and got it
pumping. He next got a beam (with, as far as I can tell, no help) and
started to make micrographs! Pretty good for someone with no EM experience,
no vacuum system experience, and no help.

When we met, Wil was looking for information as to how he could get better
micrographs. I was happy to spend a Saturday afternoon with Wil and Dr.
Sickafouse and their merry band of budding microscopists, teaching some
basic principles like gun alignment, signal maximization, vacuum
cleanliness and protocols, etc. We were able to ascertain that the SEM was
quite usable but will require a column liner dusting and cleaning so it can
be stigmated and it will require another diffusion pump cleaning and
replacement of the silicone based oil they used to begin with. (I hope they
don't have too much trouble with contamination from that - it was a
reasonable mistake for people with no Ebeam experience to make.)

My purpose here is to introduce Wil as a new microscopist who has no formal
background (so his questions won't always be worded the way those of us who
have some years in the business would do) but who has a remarkable
interest, a willing mind, and a very high level of ability to reason and
adapt - how many of us would - successfully- tackle the cleaning and
replacement of an oil diffusion pump with no more help that a typical
factory user's manual offers? How many of us could have buttoned up an old
scope after a trip across town courtesy of amateur movers and got it to
pump with no help?

To get to the purpose of this post - I can and have helped with generic
questions and all-purpose adjustments, principles and practices,
suggestions on writing a protocol so that new users can be trained and use
the instrument for various research projects. What I can't do, and what I
hope Wil and Dr. Sickafouse can find on the list, is help with their ISI
SX40 specific questions and problems. Obviously, they don't have barrels of
money and they have done very well already themselves with the nuts and
bolts side of things, but there are sure to be questions that other ISI
users and/or factory service people can help with much more expediently
than can I.

Thanks in advance to all the generous listers who will, as always, I'm
sure, help new people make it work.

Regards,
Bill Sharp
William P. Sharp
Arizona State University
Dept. Plant Biology, box 871601
Tempe, AZ 85287-1601
Phone - (480)-965-3210
Fax - (480)-965-6899



From daemon Tue Oct 30 11:49:40 2001



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Tue, 30 Oct 2001 12:40:39 -0500
Subject: Amray 1200c

Contents Retrieved from Microscopy Listserver Archives
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Here at the Central Michigan Biology Department Microscopy Facility we
have found our lab not longer requiring the services of an old SEM that
takes incredible images.. . . Lots of spare parts - contact me
directly:
willi1gl-at-cmich.edu

or check http://www.labx.com add #99928

Geoff Williams



From daemon Tue Oct 30 12:33:32 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Tue, 30 Oct 2001 12:57:46 -0600
Subject: Position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I am forwarding (with permission) a different take on electron microscopy
service. This might be an excellent approach for private industry.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/


-----Original Message-----
} From: Excalibur Mineral Co [mailto:tony-at-excaliburmineral.com]
Sent: Tuesday, October 30, 2001 8:27 AM
To: tindallr-at-missouri.edu



We are still accepting applications for the position listed below


US-IL-Chicago Northwest-Electron Microscopist -- SEM


----------------------------------------------------------------------------
----

UOP LLC, located in northwest suburban Chicago, is an international leader
in technology, products and services for the petroleum processing and
separation industries. Our R & D Deptartment, which supports fundamental and
market-focused R & D, has an immediate need for an Electron Microscopist to
work with chemists and engineers throughout the company on structural and
analytical characterization issues relating to catalytic, adsorbent, ion
exchanger and metallurgy materials.

Technical assignments will include:

- Interaction and collaboration with solid state and inorganic chemists,
applications specialists, and technical service personnel in applying
appropriate microscopic and data analysis techniques to problem solving in
catalytic and adsorbent materials fields.
- Interaction and collaboration with existing microscopy group to
effectively utilize SEM and EDXS facilities, including training of junior
technical staff.
- Development and continuation of in-house expertise in current and emerging
scanning electron microscopy techniques and applications.

Minimum qualifications include:

- BS/MS with multi-year relevant work experience, or recent PhD in Geology,
Chemistry, Materials Science, or Physics (preferred coursework in electron
microscopy, solid state chemistry and physics).
- Expertise in SEM and electron probe X-ray microanalysis techniques.
- Knowledge of sample preparation techniques, including ultramicrotomy.
- Excellent team and communication skills.

Knowledge of other microscopic characterization techniques, for example TEM
and STEM, and industrial R&D experience desirable but not essential. Above
all, we are seeking a motivated individual, who will demonstrate a
willingness to learn and openness to acquiring new areas of expertise in
characterization, chemistry and materials properties.

We offer a state-of-the-art scientific research environment coupled with
exciting career opportunities coupled with a highly competitive compensation
and benefits package including medical and dental coverage, 401(k) Plan,
Pension Plan and tuition reimbursement.

US employment authorization required.

UOP is an Equal Opportunity Employer

Visit us at www.uop.com



----------------------------------------------------------------------------
----
Additional Information
Position Type: Full Time, Employee
Ref Code: MB-01-11

----------------------------------------------------------------------------
----
Contact Information
UOP Internet Resume Manager
cro-at-uop.com
UOP LLC
25 E. Algonquin Road, P.O. Box 5017
Des Plaines IL 60017-5017
Fax: 847.391-2921

----------------------------------------------------------------------------
----

To learn more about UOP: http://company.monster.com/llc



From daemon Tue Oct 30 13:12:37 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Wed, 31 Oct 2001 09:36:57 -0500
Subject: Anthrax SEM Images

Contents Retrieved from Microscopy Listserver Archives
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Hi Dee,

I have examined polystyrene beads under the SEM. It is not difficult to do.
I applied a suspension of beads to a solid support with a pipetter, attached
the support to an SEM stub, then sputter-coated the beads with gold. Once
the sputter-coater pulls a vacuum, your solution will evaporate. I then
viewed the beads under the SEM as usual, without any special attachments.

One thing you need to be careful of is losing the beads. Unless they are
physically attached to your surface, they will not adhere to it. (Since
they are spheres, they roll off, I guess.) If you have a steady hand this
will not be a problem. The biggest challenge for me was smoothly inserting
and removing the stub with the beaded support from our sputter coater.
Several times I took the beads/stub over to another building to use the SEM.
This involved walking down several flights of stairs, and I did not lose
many beads (i.e., I saw numerous beads under the SEM.)

Hope this helps,

KD

-----Original Message-----
} From: Dee Breger [mailto:micro-at-ldeo.columbia.edu]
Sent: Monday, October 29, 2001 6:07 PM
To: microscopy-at-sparc5.microscopy.com


A few colleagues and I have an interest in viewing SEM images of anthrax
spores/virus. If someone can point me to a website that may have them in a
library accessible to me, or if someone would forward some to me, we would
greatly appreciate it.

Just so everyone is aware, this laboratory's principal function is the study
of microelectronic failure mechanisms and not in any way related to
biological technologies or biological warfare. We're simply curios.

Regards,
Peter Tomic
Anadigics, Inc.
Warren, New Jersey


From daemon Wed Oct 31 09:39:43 2001



From: Linda Jenkins :      lcjenkins-at-asub.arknet.edu
Date: Wed, 31 Oct 2001 09:28:52 -0600
Subject: LM Maintenance and Repair

Contents Retrieved from Microscopy Listserver Archives
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I am the lab coordinator here at ASUB. We have light microscopes with
100X oil immersion lenses. My supervisor wants me to attend a school of
microscopy to enable me to better maintain and repair our microscopes.
Do you know of available schools of microscopy that will meet my needs?
Thanks,
Linda




From daemon Wed Oct 31 10:27:24 2001



From: Susanne Stemmer :      stemmer-at-rice.edu
Date: Wed, 31 Oct 2001 10:19:47 -0600
Subject: electron microscopist position

Contents Retrieved from Microscopy Listserver Archives
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STAFF SCIENTIST

ELECTRON AND PROBE MICROSCOPY FACILITIES

RICE UNIVERSITY

Rice University

Rice University invites applications for a Staff Scientist position
in the newly established Center for Biological and Environmental
Nanotechnology (CBEN). Responsibilities include management of a
major electron and probe microscopy facility, training of students in
the use of the equipment, day-to-day maintenance of equipment, and
participation in the research projects at Rice. The electron
microscopy facility includes a JEOL 2010 TEM, JEOL 6320F field
emission SEM, Phillips XL30 environmental SEM, Nanoscope AFMs, a
Kamica Electron Microprobe and high-resolution XRD. This position
requires a Ph.D. in Materials Science, Physics or a related field
with specialization on electron microscopy. Preference will be given
to the applicant with (1) experience in hands-on operation and
maintenance of a range of electron and probe microscopes, (2) prior
management experience, and (3) demonstrated research experience
involving transmission electron microscopy.
Interested persons should apply (include resume, statement of
interest, list of publications, names and addresses of at least three
references) by November 15, 2001 to:

Dr. Kevin Ausman
Center for Biological and Environmental Nanotechnology
MS-63
Rice University
P.O. Box 1892
Houston, TX 77005-1892

*Rice University is an Affirmative Action/Equal Opportunity Employer*


From daemon Wed Oct 31 10:27:24 2001



From: Michael Jarnik :      M_Jarnik-at-fccc.edu
Date: Wed, 31 Oct 2001 11:15:26 -0500
Subject: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
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Listers,

I know this question was discussed in the past, but anyway. I would need
a pretty comprehensive textbook(s)/ reference book(s) covering EM use in
biology - possibly with stress on TEM, including sample preparation
techniques. Any bright ideas?

Thanks,

Michael Jarnik


--
Michael Jarnik, Ph.D.
Fox Chase Cancer Center
Electron Microscope Facility
Philadelphia



From daemon Wed Oct 31 11:00:06 2001



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Wed, 31 Oct 2001 10:52:23 -0600
Subject: Re: Anthrax SEM Images

Contents Retrieved from Microscopy Listserver Archives
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Semantics because this mistake has been making me crazy - ANTHRAX IS A
BACTERIUM! This is a particularly important point because the word "virus"
scares people silly. When I think of virus, I think of AIDS and Ebolla
(sorry it that's misspelled). When I think of bacterium, I think antibiotic.
No offense intended,
Kristen

At 09:36 AM 31-10-2001 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Kristen A. Lennon, Ph.D.
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011

515-294-8854
kalen-at-iastate.edu
www.baumlab.org



From daemon Wed Oct 31 15:37:13 2001



From: sghoshro-at-NMSU.Edu
Date: Wed, 31 Oct 2001 14:29:31 -0700 (MST)
Subject: Disposal of refrigerator

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everyone for their helpful suggestions. Given below is the
summary of all the replies I received regarding disposal of osmium
contaminated refrigerator.

****************************

You can make them happy by moving the refer next to a hood and cleaning it
with household bleach, the stronger the better. What this does is takes
the relatively safe but dark osmium dioxide residue and converts it to the
colorless but dangerous osmium tetroxide residue. It is generally
impossible to decontaminate the surfaces, just cycle between toxic and
nontoxic. The only other way is to rip out the contaminated areas of the
interior of the refer and dispose of it in a toxic landfill. I'm sure
they will appreciate this for milligram quantities of osmium.
*****************************

If there is black discolouration inside, it is due to insoluble osmium
dioxide. You can possibly remove it by sponging with diluted hydrogen
peroxide. This will reoxidise the dioxide to the tetroxide which is more
soluble and can be washed off. Of course, in doing this, you will be
exposed to osmium tetroxide solution and vapour, so you need protective
clothing. Otherwise you have to scrub off the dioxide with some kind of
abrasive cream cleaner (do you have JIF?). The dioxide is inert and
relatively harmless. BTW adding hydrogen peroxide dropwise to osmium
fixative which has gone dark will reoxidise it to the tetroxide (and
water) and restore it to useful condition.
***************************

There is often times a wide dichotomy between what safety people demand
and what common logic and good sense suggest. If it is there at all, it
would be black, right? Remember that if it was actually in the tetroxide
form, it would be colorless and you would not see it, you would smell it,
but actually it would disappear by itself because of its high vapor
pressure. So if you have areas that are black, it would be the dioxide
which is harmless. Just don't put in contact with any oxidizing agents
which would reverse the reaction back to the tetroxide. You can clean it
off however you think is the most convenient, perhaps with some laboratory
Alconox, and then you could collect the reside removed and put it aside
for recycling with your other precious metals slated for recyling and
recovery. Unfortunately, too many "safety" people are starting to actual
believe what they tell people like yourself. A few weeks ago, we had
someone like yourself being told the refrigerator had to be sent to land
fill and buried in its entirety!
*********************************

100% ethanol will reduce the OsO4 to OsO2, and the latter is a harmless
oxide.



Soumitra

*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml








From daemon Wed Oct 31 15:57:58 2001



From: akc-at-umich.edu
Date: Wed, 31 Oct 2001 16:51:47 -0500
Subject: Re: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the particularly good ones is: Bozzola JJ, Russell LD, 1991.
Electron Microscopy: Principles and Techniques for Biologist. Jones and
Bartlett Publisher (Boston), 542 pp, ISBN 0-86720-126-6.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
akc-at-umich.edu http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Wednesday, October 31, 2001 11:15 AM -0500 Michael Jarnik
{M_Jarnik-at-fccc.edu} wrote:

}
} Listers,
}
} I know this question was discussed in the past, but anyway. I would need
} a pretty comprehensive textbook(s)/ reference book(s) covering EM use in
} biology - possibly with stress on TEM, including sample preparation
} techniques. Any bright ideas?
}
} Thanks,
}
} Michael Jarnik
}
}
} --
} Michael Jarnik, Ph.D.
} Fox Chase Cancer Center
} Electron Microscope Facility
} Philadelphia
}



From daemon Wed Oct 31 16:21:24 2001



From: dmichael-at-usc.edu ()
Date: Mon, 22 Oct 2001 16:13:07 -0500
Subject: Ask-A-Microscopist: high index of refraction plastic coverslips

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dmichael-at-usc.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
October 31, 2001 at 12:13:55
---------------------------------------------------------------------------

Email: dmichael-at-usc.edu
Name: Darren Michael

Organization: University of Southern California

Education: Graduate College

Location: Los Angeles, CA

Question: Hi,

I'm trying to find high index of refraction plastic coverslips.
I've read about eyeglasses with high index of refraction (1.66)
plastic lenses and have been unable to locate a manufacturer of
coverslips using the same types of materials. Have you come across
them? Do you have any suggestions as to whom to ask?

Thanks,

Darren

---------------------------------------------------------------------------


From daemon Wed Oct 31 17:46:59 2001



From: Christopher Gieczys :      skitch007-at-hotmail.com
Date: Wed, 31 Oct 2001 18:38:29 -0500
Subject: Interactive Scheduling Software

Contents Retrieved from Microscopy Listserver Archives
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Good Evening all,

My department here is looking to acquire interactive scheduling software,
preferably for the internet, that will cover all of our microscopes. Users
should be able to create an account, manage it (add appointments, edit them,
delete them...), and be able to see what time slots are available for the
scope they wish to use. Please feel free to give me all suggestions that
come to mind as to what software is available that will accomplish this.
Thank you all very much.

Chris

_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp



From daemon Wed Oct 31 19:27:19 2001



From: Maxine Dawes :      mdawes-at-scu.edu.au
Date: Thu, 1 Nov 2001 12:19:41 +1100 (EST)
Subject: SEM - Column and Gun Valves

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Just wondering if anyone can give me some idea of the correct procedure for
closing and opening both the column and gun valves when venting a Leica 440
SEM.
Our manual only talks about closing the gun valve when using a Lab6 filament.
The manual also talks about the gun valve 'flashing' when the vacuum is ready,
and that at this point, the valve should be re-opened. Both the valves on our
machine flash upon closing them and stay flashing until we return the
leavers to
the open position We are using a Tungsten Filment not a Lab6 and we do not have
an Ion Pump on the column. We have the standard Rotary and Turbo pumps.

The manual does not explain when to close or open these valves when using a
Tungsten Filament. My question is:-
what is the correct procedure for closing the valves and re-opening them if we
are using a Tungsten Filament and not a Lab6. At present we are closing the
valves upon venting the machine in order to keep some vacuum in the column when
the chamber is at air. As soon as we close the valves they flash red. Once we
pump the column again, the gun and column valves stay flashing until we return
the leavers to the open position.




Maxine Dawes
Technical Officer
School of Environmental Science and Management
Southern Cross University
PO Box 157
Lismore, NSW, 2480

Ph: (02) 66 203661
Fx: (02) 66 212669
E-mail: mdawes-at-scu.edu.au
http://www.scu.edu.au/schools/esm/




From daemon Thu Nov 1 05:23:02 2001



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 1 Nov 2001 11:12:37 +0000
Subject: media/pH for imaging live cells

Contents Retrieved from Microscopy Listserver Archives
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I would like to do imaging of live GFP expressing mammalian cells. I
am concerned about regulating pH without needing a gas flow system on
the stage. Is adding HEPES to media sufficient to control pH in a
bicarbonate based medium? Has anyone tried the Gibco CO2 independent
medium to know if it is a high riboflavin medium (proprietary
formula so I can't look it up). Thanks- Dave
--
Permanent Address
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269-3125
knecht-at-uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html

Current Address 9/01-8/02
Dr. David Knecht
School of Biosciences
The University of Birmingham,
Edgbaston, Birmingham,
B15 2TT, U.K.
Lab telephone: 0121 414 2508 (44-121 414 2508 from abroad)
Department Fax: 0121 414 5925 (44-121 414 5925 from abroad)
Direct Fax: 0121 414 5411 (44-121 414 5411 from abroad)


From daemon Thu Nov 1 08:31:12 2001



From: Doug Cromey :      Cromey-at-Arizona.edu
Date: Tue, 23 Oct 2001 08:20:53 -0500
Subject: Re: Interactive Scheduling Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try iCal {http://www.brownbearsw.com/index.html} , it may not be
completely suitable for a large shared facility (somewhat limited use
of alternate logins), but we use it on several smaller pieces of
shared equipment (runs on NT). You can try the software as a
shareware demo.

Another possibility is {http://www.loci.wisc.edu/calendar/} , which
was described in a recent article in BioTechniques.

No commercial interest in either software.
Doug

At 06:38 PM 10/31/2001 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good Evening all,
}
} My department here is looking to acquire interactive scheduling
} software, preferably for the internet, that will cover all of our
} microscopes. Users should be able to create an account, manage it
} (add appointments, edit them, delete them...), and be able to see
} what time slots are available for the scope they wish to use.
} Please feel free to give me all suggestions that come to mind as to
} what software is available that will accomplish this.
} Thank you all very much.
}
} Chris




....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (NEW email: Cromey-at-Arizona.edu) :
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


From daemon Thu Nov 1 08:37:52 2001



From: Christopher Ogomo :      Ogomoc-at-nac.ac.za
Date: Thu, 01 Nov 2001 16:37:44 +0200
Subject: Help for freeze substitution protocols

Contents Retrieved from Microscopy Listserver Archives
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Greetings fellow listers.

I'm searching for freeze-substitution protocols (without using of any
fixative), preferably fast and slow protocols, for plant leaf specimen
preparation. I'm working on localizing nickel element in the plant leaves of
a hyperacumulator plant, hence using cryo preparation methods. I'll highly
appreciate any contributions and help in this.

My thanks in advance.

Chris Ogomo
MSc Student
University of the Western Cape
Rep of S. Africa









From daemon Thu Nov 1 08:42:02 2001



From: jshields-at-cb.uga.edu
Date: Thu, 1 Nov 2001 09:37:22 -0500
Subject: Re: citfluor

Contents Retrieved from Microscopy Listserver Archives
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citifluor:
Ted Pella
800-237-3526
www.tedpella.com

On 1 Nov 2001, at 15:08, Kathrine Andersen wrote:

} Hi,
}
} can anybody tell me where to order "citfluor"( mounting medium)?
} Thanks in advance!
}
} Kathrine




From daemon Thu Nov 1 08:43:45 2001



From: Chen Chen :      cche1-at-mail.jhmi.edu
Date: Thu, 1 Nov 2001 09:21:20 -0500 (EST)
Subject: Re: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yeah, this book is really a good book for beginners. but there is now second
edition in 1999 available 670pp, ISBN 0-7637-0192-0. The content is from basic
structure and principles of TEM and SEM to sample preparation with many vivid
pictures, mainly for
tissue samples, also some pages on FT. This book is very basic, but not
advanced,
and it is quite cheap($8) .

Chen Chen

Department of Biological Chemistry
The Johns Hopkins University
School of Medicine
725 N. Wolfe Street
Baltimore, Maryland 21218

On Wed, 31 Oct 2001 akc-at-umich.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} One of the particularly good ones is: Bozzola JJ, Russell LD, 1991.
} Electron Microscopy: Principles and Techniques for Biologist. Jones and
} Bartlett Publisher (Boston), 542 pp, ISBN 0-86720-126-6.
}
} Kent
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} A. Kent Christensen, Professor Emeritus
} Department of Cell and Developmental Biology, Medical Science II Building
} University of Michigan Medical School, Ann Arbor, MI 48109-0616
} Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
} akc-at-umich.edu http://www.umich.edu/~akc/
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} --On Wednesday, October 31, 2001 11:15 AM -0500 Michael Jarnik
} {M_Jarnik-at-fccc.edu} wrote:
}
} }
} } Listers,
} }
} } I know this question was discussed in the past, but anyway. I would need
} } a pretty comprehensive textbook(s)/ reference book(s) covering EM use in
} } biology - possibly with stress on TEM, including sample preparation
} } techniques. Any bright ideas?
} }
} } Thanks,
} }
} } Michael Jarnik
} }
} }
} } --
} } Michael Jarnik, Ph.D.
} } Fox Chase Cancer Center
} } Electron Microscope Facility
} } Philadelphia
} }
}
}



From daemon Thu Nov 1 08:48:32 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Thu, 01 Nov 2001 09:41:12 -0500
Subject: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

I am working on a multidisciplinary team of researchers on a number of very interesting projects. I have a very large collection of top quality micrographs from these projects which I would like to publish. The microscopy samples have been selected and photographed within the experimental design which has been set out for each project, so we are pretty sure they are representative of what's there.

One collaborator on the team is hesitant (to the point of being resistant) about my publishing any of the microscopy as part of any of the publications put together by the team because we have not looked at 30-40 samples of each of the many experimental treatments ( we have done 4 samples of each treatment) . The other researchers on the team have looked at between 30 and 40 samples per treatment. This one collaborator says that she has no confidence in the microscopy results because "the statistics just aren't there".

I have tried on numberous occasions to explain to her about why this is not feasible to do in SEM, and is usually not done. She does not believe me and is essentially preventing me from publishing the work. I could probably publish this work as a stand alone ms, but it is so much more meaningful when integrated with data from the other analytical techniques.

My question: is there a reference (which I have somehow missed) to explain why 30-40 samples is not feasible for microscopy and which rationalizes why this is so? The function of microscopy in the projects has been to give pictorial information about the samples, and to suggest trends, not for absolute measurement of structures and changes in them.

Any help you can provide would be very much appreciated - before I rip all of my hair out....

Thanks in advance, listers, to all of you. I know I came to the right place.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Nov 1 09:22:40 2001



From: Joyce Craig :      J-Craig-at-csu.edu
Date: Thu, 01 Nov 2001 09:07:29 -0800
Subject: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy by John Bozzola and Lonnie Russell is excellent. I
use it for both my TEM and SEM class.
Biological Electron Microscopy by Michael J. Dykstra is also useful. It
is more compact in size so easier om students' backs, and still seems to
cover the necessary material. It doesn't include the extensive
ultrastructure section Bozzola's book has.
Either is good, and there are also many other good books out there.
I have always spent a lot of time at the library display at the MSA
meetings.
Joyce Craig
Chicago State University



From daemon Thu Nov 1 09:25:43 2001



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Thu, 01 Nov 2001 09:22:37 -0600
Subject: bacillus anthracis

Contents Retrieved from Microscopy Listserver Archives
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Hello,
The following point is more so semantics.

Anthrax is actually the disease caused by the spore-forming bacterium
Bacillus anthracis. The bacterium in question is actually named Bacillus
anthracis. I guess it is easier for the news media to say anthrax than
bacillus anthracis.

Tyrone Daulton


Kristen Lennon wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Semantics because this mistake has been making me crazy - ANTHRAX IS A
} BACTERIUM! This is a particularly important point because the word "virus"
} scares people silly. When I think of virus, I think of AIDS and Ebolla
} (sorry it that's misspelled). When I think of bacterium, I think antibiotic.
} No offense intended,
} Kristen
}
} Kristen A. Lennon, Ph.D.
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
}
} 515-294-8854
} kalen-at-iastate.edu
} www.baumlab.org

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Thu Nov 1 09:53:35 2001



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Thu, 1 Nov 2001 15:47:59 -0000
Subject: Microlumina Software lost

Contents Retrieved from Microscopy Listserver Archives
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Dear Collegues. I Have a Microlumina camera in my lab and lost the EasyScan
1.2 software as a result of a virus attack. The original diskas are by now
unreadable.
Does anyone have a copy of this software? I would be eternally gratefull if
someone could send me a copy by e-mail to the address below. I think that
the camera has been descontinued and is no longer available comercially.


Thanks in advance
Dr. A.P. Alves de Matos
Lisbon University Dental Medical School
apmatos-at-ip.pt



From daemon Thu Nov 1 09:53:44 2001



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu
Date: Thu, 01 Nov 2001 11:45:10 -0500
Subject: sudan black

Contents Retrieved from Microscopy Listserver Archives
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Dear listmembers,

Does anyone have a protocol for staining lipid droplets in cells using Sudan
black? (Or any other lipid-specific stain?) Thanks for looking.

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Thu Nov 1 10:37:34 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 01 Nov 2001 08:28:57 -0800
Subject: Re: need sem protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dee,
When I looked at latex spheres made in our Pathology department, I just
dried a drop out of the suspension onto a polished graphite planchet. A
glass cover slip is also a good substrate, if you are going to gold-coat
anyway. If the carrier is really DI, it should dry away clean. If there is
stuff in the DI, you will get smut around the spheres. I believe the
polystyrene is strong enough to hold up to drying. If the spheres are small
enough, they stick to the graphite themselves. If they are larger and in
danger of rolling off, dry them onto a sticky tab.
At 06:07 PM 10/29/01 -0500, you wrote:
}
} Hi listers,
}
} I've been asked to look at some polystyrene microspherules in the SEM and
} would like some advice on how to prepare them. I'm not a biomedical
} person, so need some help!
}
} The spherules are 5.6 um size with a Lumidin (protein) bonding surface and
} receptor molecules attached. They're in DI (or a DI solution?), which is
} apparently the delivery system for the marker molecules. Would they need
} CPD? If so, I've never CPD'd particles in solution before.... The SEM has
} hivac and VP modes, plus there's a cool stage.
}
} Any ideas would be highly appreciated!
} Many thanks,
} Dee
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Nov 1 11:20:12 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 1 Nov 2001 12:11:31 -0500
Subject: RE: Ask-A-Microscopist: high index of refraction plastic coversli

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Darren,
Under separate cover I am sending an Excel table with plastics,
their monomers and their indices of refraction. At least this may help you
look.

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: dmichael-at-usc.edu
} Sent: Monday, October 22, 2001 5:13 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: high index of refraction plastic
} coverslips
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (dmichael-at-usc.edu) from
} http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
} October 31, 2001 at 12:13:55
} --------------------------------------------------------------------------
} -
}
} Email: dmichael-at-usc.edu
} Name: Darren Michael
}
} Organization: University of Southern California
}
} Education: Graduate College
}
} Location: Los Angeles, CA
}
} Question: Hi,
}
} I'm trying to find high index of refraction plastic coverslips.
} I've read about eyeglasses with high index of refraction (1.66)
} plastic lenses and have been unable to locate a manufacturer of
} coverslips using the same types of materials. Have you come across
} them? Do you have any suggestions as to whom to ask?
}
} Thanks,
}
} Darren
}
} --------------------------------------------------------------------------
} -
}
}


From daemon Thu Nov 1 11:58:48 2001



From: R. Ann Bliss :      bliss5-at-popcorn.llnl.gov
Date: Thu, 1 Nov 2001 09:52:03 -0800
Subject: Microtome of teeth

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

HAPPY NOVEMBER!

And, can anyone give me some pointers on the microtoming the teeth of
a mouse? We have a Leica Ultracut UCT. Please reply directly to me.

--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Thu Nov 1 12:53:43 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 1 Nov 2001 12:45:17 -0600
Subject: TEM: Mucin fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Does anyone know of a most excellent method of fixing the mucin layers of a
cell culture for TEM? There is a rumor of some osmium/fluoride compound
that I'm trying to track down (osmium perfluouro-something?), but no luck
yet. Personal experiences, references, and sources for exotic compounds
would all be most welcome.

Thanks much.

Randy


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Nov 1 15:11:43 2001



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Thu, 1 Nov 2001 16:01:37 -0500
Subject: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you are teaching biological TEM, then you might consider using as a
corollary "Cell and Tissue Ultrastructure: A Functional Perspective" by
Cross and Mercer, pub by W.H.Freeman & Co NY. No sample prep or EM
operation, but beautifully-presented micrographs illustrating ultrastructure
and tissue types.

A.L.

#######################################
Ann Hein Lehman
Assistant Director, EM Facility
Trinity College - LSC314
300 Summit Street
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman/

-----Original Message-----
} From: Joyce Craig [mailto:J-Craig-at-csu.edu]
Sent: Thursday, November 01, 2001 12:07 PM
To: Microscopy microscopy


Electron Microscopy by John Bozzola and Lonnie Russell is excellent. I
use it for both my TEM and SEM class.
Biological Electron Microscopy by Michael J. Dykstra is also useful. It
is more compact in size so easier om students' backs, and still seems to
cover the necessary material. It doesn't include the extensive
ultrastructure section Bozzola's book has.
Either is good, and there are also many other good books out there.
I have always spent a lot of time at the library display at the MSA
meetings.
Joyce Craig
Chicago State University



From daemon Thu Nov 1 15:11:43 2001



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 01 Nov 2001 15:00:34 -0600
Subject: Re: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 09:21 AM 11/1/01 -0500, Chen Chen wrote:
} Yeah, this book is really a good book for beginners. but there is now second
} edition in 1999 available 670pp, ISBN 0-7637-0192-0. The content is from basic
} structure and principles of TEM and SEM to sample preparation with many vivid
} pictures, mainly for
} tissue samples, also some pages on FT. This book is very basic, but not
} advanced,
} and it is quite cheap($8) .

Amazon shows it at $83, not $8:

http://www.amazon.com/exec/obidos/ASIN/0763701920/qid%3D/104-2645789-0245565

- John



From daemon Thu Nov 1 15:25:51 2001



From: Sara Miller :      saram-at-duke.edu
Date: Thu, 1 Nov 2001 16:17:50 -0500 (EST)
Subject: Re: TEM: Mucin fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Not sure about mucin, but have you looked into ruthenium red????

On Thu, 1 Nov 2001, Tindall, Randy D. wrote:

} Date: Thu, 1 Nov 2001 12:45:17 -0600
} From: Tindall, Randy D. {TindallR-at-missouri.edu}
} To: "'microscopy-at-sparc5.microscopy.com'"
{microscopy-at-sparc5.microscopy.com}
} Subject: TEM: Mucin fixation
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} Does anyone know of a most excellent method of fixing the mucin layers of a
} cell culture for TEM? There is a rumor of some osmium/fluoride compound
} that I'm trying to track down (osmium perfluouro-something?), but no luck
} yet. Personal experiences, references, and sources for exotic compounds
} would all be most welcome.
}
} Thanks much.
}
} Randy
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Thu Nov 1 16:08:50 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 1 Nov 2001 16:06:27 -0600
Subject: Re: Biology EM textbook

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No one has mentioned this book, so I might as well:
"Biomedical Electron Microscopy" by A.B. Maunsbach and B.A. Afzelius
Academic Press, 1999, ISBN 0-12-480610-4
This text has many EMs comparing the effects of using different
fixatives, buffers, embedding resins, and so forth. An excellent
companion to Bozzola & Russell.
Note: check ebay. I got my copy there for about 2/3 of the retail price.

Phil

} Electron Microscopy by John Bozzola and Lonnie Russell is excellent. I
} use it for both my TEM and SEM class.
} Biological Electron Microscopy by Michael J. Dykstra is also useful. It
} is more compact in size so easier om students' backs, and still seems to
} cover the necessary material. It doesn't include the extensive
} ultrastructure section Bozzola's book has.
} Either is good, and there are also many other good books out there.
} I have always spent a lot of time at the library display at the MSA
} meetings.
} Joyce Craig
} Chicago State University

--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Nov 1 16:59:20 2001



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Thu, 01 Nov 2001 16:54:21 -0600
Subject: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Paula,
How many samples you need to examine depends on the details of the experiment, i.e. nature of specimens, the types of measurements taken, and most importantly the experimental question that is addressed by the experiments. The question to whether you have significant statistics is dictated by these enumerated, and perhaps other, factors. These factors will obviously differ for different experiments which have different goals.

My advice is to sit down and examine closely the experimental questions you choose to address and decide whether the data you have collected has the necessary statistics to adequately constrain the interpretations of the data.

The only meaningful reference (of which you seek) would be one that discusses measurement statistics on similar experiments, taking similar measurements, and addressing similar questions.

I hope this is of some help, good luck.
Ty



Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, all,
}
} I am working on a multidisciplinary team of researchers on a number of very interesting projects. I have a very large collection of top quality micrographs from these projects which I would like to publish. The microscopy samples have been selected and photographed within the experimental design which has been set out for each project, so we are pretty sure they are representative of what's there.
}
} One collaborator on the team is hesitant (to the point of being resistant) about my publishing any of the microscopy as part of any of the publications put together by the team because we have not looked at 30-40 samples of each of the many experimental treatments ( we have done 4 samples of each treatment) . The other researchers on the team have looked at between 30 and 40 samples per treatment. This one collaborator says that she has no confidence in the microscopy results because "the statistics just aren't there".
}
} I have tried on numberous occasions to explain to her about why this is not feasible to do in SEM, and is usually not done. She does not believe me and is essentially preventing me from publishing the work. I could probably publish this work as a stand alone ms, but it is so much more meaningful when integrated with data from the other analytical techniques.
}
} My question: is there a reference (which I have somehow missed) to explain why 30-40 samples is not feasible for microscopy and which rationalizes why this is so? The function of microscopy in the projects has been to give pictorial information about the samples, and to suggest trends, not for absolute measurement of structures and changes in them.
}
} Any help you can provide would be very much appreciated - before I rip all of my hair out....
}
} Thanks in advance, listers, to all of you. I know I came to the right place.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Thu Nov 1 17:28:40 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Thu, 01 Nov 2001 17:20:23 -0600
Subject: Re: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

Even statistically speaking, the number of samples that you need depends
on the variability between samples in your group and the extent to
which the experimental group varies from the controls.

If your experimental group consistently looks one way and your controls
consistently and obviously look different from the experimental group,
I don't see why you would need to run statistics.

But it really depends on what you are trying to say. If all you are
trying to point out is group one looks different than normal and it is
obvious in all of your samples, (by obvious, I mean obvious to a
trained eye) statistics is a waste of time. However, when you start to
say group one looks somewhat worse than group two which looks somewhat
worse than normal, and some of the specimens in the group actually
look the same as, or better than ,the ones in the better group, then
you'd need statistics. There are ways to figure out how big the group
actually needs to be by taking a sample and determining the variability
within and between groups (say on your 4 samples already obtained).
I'm betting if you really can see something with these few samples,
the estimation of sample size needed for statistics will be allot less
than 30-40.

Unfortunately, I don't know of any articles you can site about
the use of statistics in SEM off hand. Sorry. I've personally run into
people recommending these ridiculously large sample sizes because they
work with people. I work with animals and have allot more control over
inter subject variability than they do, therefore I can work with a
much smaller sample size even for statistics.

Good luck,

Karen Pawlowski, Ph.D.


Paula Allan-Wojtas wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, all,
}
} I am working on a multidisciplinary team of researchers on a number of very interesting projects. I have a very large collection of top quality micrographs from these projects which I would like to publish. The microscopy samples have been selected and photographed within the experimental design which has been set out for each project, so we are pretty sure they are representative of what's there.
}
} One collaborator on the team is hesitant (to the point of being resistant) about my publishing any of the microscopy as part of any of the publications put together by the team because we have not looked at 30-40 samples of each of the many experimental treatments ( we have done 4 samples of each treatment) . The other researchers on the team have looked at between 30 and 40 samples per treatment. This one collaborator says that she has no confidence in the microscopy results because "the statistics just aren't there".
}
} I have tried on numberous occasions to explain to her about why this is not feasible to do in SEM, and is usually not done. She does not believe me and is essentially preventing me from publishing the work. I could probably publish this work as a stand alone ms, but it is so much more meaningful when integrated with data from the other analytical techniques.
}
} My question: is there a reference (which I have somehow missed) to explain why 30-40 samples is not feasible for microscopy and which rationalizes why this is so? The function of microscopy in the projects has been to give pictorial information about the samples, and to suggest trends, not for absolute measurement of structures and changes in them.
}
} Any help you can provide would be very much appreciated - before I rip all of my hair out....
}
} Thanks in advance, listers, to all of you. I know I came to the right place.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca


From daemon Thu Nov 1 17:31:44 2001



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Thu, 01 Nov 2001 15:24:27 -0800
Subject: Re: Help for freeze substitution protocols

Contents Retrieved from Microscopy Listserver Archives
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Chris,

We regularly use 1% osmium tetroxide with 8% dimethoxypropane (DMP) in
acetone for woody plant tissues with good success. The DMP removes water
from the solution. We substitute for 5 days at -80 C. Anything less that 3
days won't work at all and more than 5 days makes no difference. We have
used straight acetone for immuno work but the fixation (i.e. membranes) is
not so great and the images are low in contrast. Other possibilities are 0.2
to 2% glutaraldehyde in acetone, with or without tannic acid. This is good
for protein preservation for immuno work. Methanol is reported to work but
not as well as acetone (I haven't successfully used it).

Let me know if I can be of further help.

Kim
-------
Kim Rensing Ph.D.
Dept. of Botany, UBC
6270 University Blvd
Vancouver, BC, Canada
V6T 1Z4

} I'm searching for freeze-substitution protocols (without using of any
} fixative), preferably fast and slow protocols, for plant leaf specimen
} preparation. I'm working on localizing nickel element in the plant leaves of
} a hyperacumulator plant, hence using cryo preparation methods. I'll highly
} appreciate any contributions and help in this.
}
} My thanks in advance.
}
} Chris Ogomo
} MSc Student
} University of the Western Cape
} Rep of S. Africa



From daemon Thu Nov 1 18:27:14 2001



From: griggsa-at-dbcc.cc.fl.us ()
Date: Tue, 23 Oct 2001 18:18:09 -0500
Subject: Ask-A-Microscopist: oculars for use by visually impaired

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (griggsa-at-dbcc.cc.fl.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
November 1, 2001 at 14:26:59
---------------------------------------------------------------------------

Email: griggsa-at-dbcc.cc.fl.us
Name: Alvin Griggs

Education: Undergraduate College

Location: Daytona Beach, FL, USA

Question: I am looking for places that sell or lease microscope
projection oculars for use by visually impaired students.

---------------------------------------------------------------------------


From daemon Thu Nov 1 23:51:17 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 02 Nov 2001 00:41:21 -0500
Subject: Re: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 11/1/01 9:41 AM, Paula Allan-Wojtas at AllanWojtasP-at-EM.AGR.CA wrote:

} I am working on a multidisciplinary team of researchers on a number of very
} interesting projects. I have a very large collection of top quality
} micrographs from these projects which I would like to publish. The microscopy
} samples have been selected and photographed within the experimental design
} which has been set out for each project, so we are pretty sure they are
} representative of what's there.
}
} One collaborator on the team is hesitant (to the point of being resistant)
} about my publishing any of the microscopy as part of any of the publications
} put together by the team because we have not looked at 30-40 samples of each
} of the many experimental treatments ( we have done 4 samples of each
} treatment) . The other researchers on the team have looked at between 30 and
} 40 samples per treatment. This one collaborator says that she has no
} confidence in the microscopy results because "the statistics just aren't
} there".
}
} I have tried on numberous occasions to explain to her about why this is not
} feasible to do in SEM, and is usually not done. She does not believe me and
} is essentially preventing me from publishing the work. I could probably
} publish this work as a stand alone ms, but it is so much more meaningful when
} integrated with data from the other analytical techniques.
}
} My question: is there a reference (which I have somehow missed) to explain why
} 30-40 samples is not feasible for microscopy and which rationalizes why this
} is so? The function of microscopy in the projects has been to give pictorial
} information about the samples, and to suggest trends, not for absolute
} measurement of structures and changes in them.
}
} Any help you can provide would be very much appreciated - before I rip all of
} my hair out....
}
Dear Paula,
As others have said, it depends on what kind of experiment you're
looking at. Even if all the experimental samples look different from the
controls, if you're trying to quantitate the changes--e.g., adding X to the
culture shrinks the cells by Y%--you may still need sufficient data to make
the numbers significant and to provide a standard deviation. Is is also
important to have looked at different, independently-prepared samples, so if
the few samples you observed were all from one experiment, I, too, would
have doubts that they were sufficient. Then there's the problem that one
selects what to photograph, and this selection always involves subjective
factors. All that being said, however, if each of the other properties
measured in the experiment were consistent from prep to prep, and if what
you see correlates with what was measured, then, IMHO, it would be proper to
publish the micrographs to illustrate what was found, regardless of how many
or few you have. Good luck.
Yours,
Bill Tivol




From daemon Fri Nov 2 02:04:57 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Fri, 2 Nov 2001 07:57:35 -0000
Subject: Re: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula
Most of us have grappled with this problem, but there is no generally
applicable answer to the question "How many samples do I need to
examine?". You are correct in saying that most electron
microscopists don't attempt to examine 30-40 samples per treatment,
but sometimes that is unavoidable. If the scientific question you are
asking is a quantitative one - e.g. what proportion of cells have a
particular morphology in normal and diseased tissues - and especially
if the legal or public health or commercial cost implications of the
answer are very significant - then it may be necessary to examine the
number of samples and analyse the number of photographs demanded by
the statisticians. If on the other hand you just want to illustrate
the effect of a particular mutation on the shape of a fly's eye 2-3
representative samples are probably adequate. I feel hampered in
making any really helpful suggestions about your experiment because it
is not clear from your message what the nature of the experiment is,
or what kind of observations you are attempting to make. Could you
tell us more about that?

Best wishes
Chris


} Paula Allan-Wojtas wrote:
}
}
} --------------------------------------------------------------------
----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
---.
} }
} } Hi, all,
} }
} } I am working on a multidisciplinary team of researchers on a
number of very interesting projects. I have a very large collection of
top quality micrographs from these projects which I would like to
publish. The microscopy samples have been selected and photographed
within the experimental design which has been set out for each
project, so we are pretty sure they are representative of what's
there.
} }
} } One collaborator on the team is hesitant (to the point of being
resistant) about my publishing any of the microscopy as part of any of
the publications put together by the team because we have not looked
at 30-40 samples of each of the many experimental treatments ( we have
done 4 samples of each treatment) . The other researchers on the team
have looked at between 30 and 40 samples per treatment. This one
collaborator says that she has no confidence in the microscopy results
because "the statistics just aren't there".
} }
} } I have tried on numberous occasions to explain to her about why
this is not feasible to do in SEM, and is usually not done. She does
not believe me and is essentially preventing me from publishing the
work. I could probably publish this work as a stand alone ms, but it
is so much more meaningful when integrated with data from the other
analytical techniques.
} }
} } My question: is there a reference (which I have somehow missed) to
explain why 30-40 samples is not feasible for microscopy and which
rationalizes why this is so? The function of microscopy in the
projects has been to give pictorial information about the samples, and
to suggest trends, not for absolute measurement of structures and
changes in them.
} }
} } Any help you can provide would be very much appreciated - before
I rip all of my hair out....
} }
} } Thanks in advance, listers, to all of you. I know I came to the
right place.
} }
} } Paula.
} }
} } Paula Allan-Wojtas
} } Research Scientist - Food Microstructure
} } Agriculture and Agri-Food Canada
} } Atlantic Food and Horticulture Research Centre
} } Kentville, Nova Scotia Canada B4N 1J5
} }
} } Tel: (902) 679-5566
} } FAX: (902) 679-2311
} }
} } email: allanwojtasp-at-em.agr.ca




From daemon Fri Nov 2 03:51:53 2001



From: vcr.group-vince-at-worldnet.att.net
Date: Fri, 2 Nov 2001 04:43:12 -0500 (EST)
Subject: NYTimes.com Article: Particles Are Tiny, but Damage Can Be Great

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This article from NYTimes.com
has been sent to you by vcr.group-vince-at-worldnet.att.net.



Particles Are Tiny, but Damage Can Be Great

October 30, 2001

By JAMES GLANZ


Environmental scientists have spent decades studying the
physics and physiology of particles very much like those in
the most dangerous forms of biological weaponry.

http://www.nytimes.com/2001/10/30/science/earth/30PART.html?ex=1005694192&ei=1&en=7eed26d952d00beb



HOW TO ADVERTISE
---------------------------------
For information on advertising in e-mail newsletters
or other creative advertising opportunities with The
New York Times on the Web, please contact Alyson
Racer at alyson-at-nytimes.com or visit our online media
kit at http://www.nytimes.com/adinfo

For general information about NYTimes.com, write to
help-at-nytimes.com.

Copyright 2001 The New York Times Company


From daemon Fri Nov 2 07:44:01 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Fri, 02 Nov 2001 08:31:12 -0500
Subject: SEM - how many samples are enough - Round 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, again,

Thank you to everyone who has responded, so far. It has been very helpful for me. The responses have covered the whole spectrum from "yes, do the 30-40 samples" to "3-4 is probably enough". The majority of the answers fell in between these 2 points, saying that the number of samples required to be representative really depends on what you want/need to know from the samples and the sample variability. This could be partially achieved by taking pictures randomly over different samples. I will post a summary of answers soon. Anyone who does not want me to reveal their reply/name please contact me offline to let me know.

In some other unrelated work that we have done recently, we took the approach of working with a statistician from the outset. The project was a much more focused one, and we were looking for treatment effects, and intensity of these. One of the first steps was to actually do a small study to assess the variability of the population, and factors which affected it. With this information we were able to move to the next phase, actually testing the treatments. Statistics (experimental design, proper data collection and analysis of the data) allowed us to separate the population variability from the treatment effects, even when they were subtle. The result was information which did not agree with published literature or longheld assumptions which had arrived at through studies that did not have the "statistical rigour" that our studies did. Sorry to be vague about this, but it will be some months before I can openly talk about this work. I just wanted to say, for the record, that I am not against a statistical approach. I think it's a great idea, and I think it's the way to go.

In the original work I contacted the group about yesterday, we were just making some preliminary observations about the samples and suggesting that a connection could be made between the microscopy and other analytical methods. I have published other papers of this type with other workgroups, and everyone was ok with the "representative micrographs" idea, provided the usual disclaimers were used - "possible relationship" rather than "correlation"; "preliminary observations" ;More work should be done with more samples to be certain we're seeing what we think we're seeing " a first attempt at trying to relate microstructure to..."

I really can't go into more detail about this situation for obvious reasons. Suffice it to say that I plan to do my microscopy from now on in a new frame of mind, and will take some time to speak with a statistician before I start any new projects. My quest is to get the work I originally described, and all the work that we did connected to it, published in some form (complete with all the disclaimers necessary) as soon as I can.

Thanks again for all your help and support.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Fri Nov 2 07:44:01 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Fri, 2 Nov 2001 09:38:34 -0400
Subject: RE: need sem protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been looking at marine algae in the same size range for quite some
time. One of the considerations is having the sample distributed well
enough to see individual particles and have a uniform random distribution
for statistical purposes. Drying down from a drop of liquid tends to bunch
up the particles and distribute the size ranges non-uniformly. We have used
polycarbonate filters (Nuclepore, Poretics) and filter from liquid solution
to collect the particles on a low profile background.

There are a number of pore sizes to choose from, select the one just below
the size range of interest so that smaller detritus is removed.
Try a dilution series to get a useable concentration.
If the particles have enough inherent rigidity so they do not collapse, air
drying is sufficient.
We mount the filters on SEM stubs while still somewhat damp (they will fly
off with static and/or handling of the flimsy material when completely dry).
We then coat with gold. The one caution here is when the vacuum is applied
and released make sure that it is done slowly so that large bursts of air
don't sweep over the particles.
Having said that our experience is that micron sized particles tend to stick
to the surface they lay on.
If you are adventurous you might try looking at the particles uncoated in VP
mode.

Hope this helps.

-David O'Neil
Institute for Marine Biosciences
National Research Council of Canada
1411 Oxford St.
Halifax, NS B3H 3Z1
ph. 902-426-8258
fax 902-426-9413
david.o'neil-at-nrc.ca
http://www.imb.nrc.ca/micros_e.html



At 06:07 PM 10/29/01 -0500, you wrote:
}
} Hi listers,
}
} I've been asked to look at some polystyrene microspherules in the SEM and
} would like some advice on how to prepare them. I'm not a biomedical
} person, so need some help!
}
} The spherules are 5.6 um size with a Lumidin (protein) bonding surface and
} receptor molecules attached. They're in DI (or a DI solution?), which is
} apparently the delivery system for the marker molecules. Would they need
} CPD? If so, I've never CPD'd particles in solution before.... The SEM has
} hivac and VP modes, plus there's a cool stage.
}
} Any ideas would be highly appreciated!
} Many thanks,
} Dee




From daemon Fri Nov 2 08:41:40 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 02 Nov 2001 09:36:01 -0500
Subject: Re: TEM: Mucin fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Tindall, Randy D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} Does anyone know of a most excellent method of fixing the mucin layers of a
} cell culture for TEM? There is a rumor of some osmium/fluoride compound
} that I'm trying to track down (osmium perfluouro-something?), but no luck
} yet. Personal experiences, references, and sources for exotic compounds
} would all be most welcome.
}
} Thanks much.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/

Ruthenium red, as Sara Miller wrote, works well. Osmium can be dissolved in
organic compounds, the idea being not to expose the object of your study to
aqueous solvents while it is being fixed. I don't know how well osmium fixes
the mucin you are interested in. Glutaraldehyde and formaldehyde can also be
"dissolved" or partitioned into organic solvents to accomplish the same end.
This is known as phase partition fixation. I will send a reference if you like.
Of course, if you are working with plastic culture dishes, the organic solvent
must not react with the dish.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Nov 2 08:44:12 2001



From: Russell McConnell :      russell.mcconnell-at-tufts.edu
Date: Fri, 02 Nov 2001 09:38:38 -0500
Subject: Re: scheduling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} } } Dear Christopher,
} } } The software that we use for scheduling is called Webevent. It is
} } } fairly straightforward to use, and people can set up accounts and edit
} } } them, depending on how you set it up. Their web site is:
} } } http://www.webevent.com
} } } Hope that helps.
} } }
} } } -Russell
} } }
} } } Christopher Gieczys wrote:
} } } }

} } } }
} } } } Good Evening all,
} } } }
} } } } My department here is looking to acquire interactive scheduling software,
} } } } preferably for the internet, that will cover all of our
} } } microscopes. Users
} } } } should be able to create an account, manage it (add
} } } appointments, edit them,
} } } } delete them...), and be able to see what time slots are available for the
} } } } scope they wish to use. Please feel free to give me all suggestions that
} } } } come to mind as to what software is available that will accomplish this.
} } } } Thank you all very much.
} } } }
} } } } Chris
} } } }
} } } } _________________________________________________________________
} } } } Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp


--
Russell McConnell
Confocal Imaging Facility Technician
Department of Neuroscience
Tufts University School of Medicine
M&V Building room #137
136 Harrison Ave.
Boston, MA 02111


From daemon Fri Nov 2 08:47:50 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 2 Nov 2001 09:42:32 -0500
Subject: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are my two cents.

If you are not quantifying the microstructure with stereological measurements, then you use your qualitative judgment as to whether the micrographs that you use are representative. Typically you will get a feel for it. If on the other hand, you are using quantitative microscopy techniques, there are statistical tests that you judge your results by. These tests will tell you whether your sampling is sufficient. I recommend that you look at several books on the topic. The materials science oriented books that I am familiar with are DeHoff and Rhines "Quantitative Microscopy" and John Russ's books; try the "Image Processing Handbook" for example. John runs a short course on this topic every year and DeHoff is a co-instructor.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Tyrone L. Daulton [mailto:tdaulton-at-nrlssc.navy.mil]
Sent: Thursday, November 01, 2001 5:54 PM
To: Paula Allan-Wojtas; microscopy-at-sparc5.microscopy.com


Hello Paula,
How many samples you need to examine depends on the details of the experiment, i.e. nature of specimens, the types of measurements taken, and most importantly the experimental question that is addressed by the experiments. The question to whether you have significant statistics is dictated by these enumerated, and perhaps other, factors. These factors will obviously differ for different experiments which have different goals.

My advice is to sit down and examine closely the experimental questions you choose to address and decide whether the data you have collected has the necessary statistics to adequately constrain the interpretations of the data.

The only meaningful reference (of which you seek) would be one that discusses measurement statistics on similar experiments, taking similar measurements, and addressing similar questions.

I hope this is of some help, good luck.
Ty



Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, all,
}
} I am working on a multidisciplinary team of researchers on a number of very interesting projects. I have a very large collection of top quality micrographs from these projects which I would like to publish. The microscopy samples have been selected and photographed within the experimental design which has been set out for each project, so we are pretty sure they are representative of what's there.
}
} One collaborator on the team is hesitant (to the point of being resistant) about my publishing any of the microscopy as part of any of the publications put together by the team because we have not looked at 30-40 samples of each of the many experimental treatments ( we have done 4 samples of each treatment) . The other researchers on the team have looked at between 30 and 40 samples per treatment. This one collaborator says that she has no confidence in the microscopy results because "the statistics just aren't there".
}
} I have tried on numberous occasions to explain to her about why this is not feasible to do in SEM, and is usually not done. She does not believe me and is essentially preventing me from publishing the work. I could probably publish this work as a stand alone ms, but it is so much more meaningful when integrated with data from the other analytical techniques.
}
} My question: is there a reference (which I have somehow missed) to explain why 30-40 samples is not feasible for microscopy and which rationalizes why this is so? The function of microscopy in the projects has been to give pictorial information about the samples, and to suggest trends, not for absolute measurement of structures and changes in them.
}
} Any help you can provide would be very much appreciated - before I rip all of my hair out....
}
} Thanks in advance, listers, to all of you. I know I came to the right place.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Fri Nov 2 08:50:33 2001



From: Karen Pawlowski :      kpawlow-at-swbell.net
Date: Fri, 02 Nov 2001 08:45:07 -0600
Subject: Re: TEM: Mucin fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Or you could try Alcian blue/glutaraldehyde followed by osmium
textroxide. See:
Hear Research 1987;27(1):47-65 A newly identified surface coat on
cochlear hair cells. Santi PA, Anderson CB.

Warning: This method is tricky because it can end up trapping
water molecules in the mucous type tissues and it requires longer
dehydration and polymerization times to set blocks hard enough for
EM. But it looks great! (Actually, the molecules don't collapse as
much in this as in ruthenium red, so the "coat" is closer to it's
true thickness.) I only did some light level stuff with this myself,
but I worked down the hall from Dr. Santi when this experiment was
being done.

Karen Pawlowski

Sara Miller wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Not sure about mucin, but have you looked into ruthenium red????
}
} On Thu, 1 Nov 2001, Tindall, Randy D. wrote:
}
} } Date: Thu, 1 Nov 2001 12:45:17 -0600
} } From: Tindall, Randy D. {TindallR-at-missouri.edu}
} } To: "'microscopy-at-sparc5.microscopy.com'"
} {microscopy-at-sparc5.microscopy.com}
} } Subject: TEM: Mucin fixation
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} }
} } Does anyone know of a most excellent method of fixing the mucin layers of a
} } cell culture for TEM? There is a rumor of some osmium/fluoride compound
} } that I'm trying to track down (osmium perfluouro-something?), but no luck
} } yet. Personal experiences, references, and sources for exotic compounds
} } would all be most welcome.
} }
} } Thanks much.
} }
} } Randy
} }
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
} }
} }
} }
} }
} }
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265


From daemon Fri Nov 2 09:38:21 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 2 Nov 2001 10:28:24 -0500
Subject: Re: Mucin and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,
Sorry for the bulk of this response, but finding what you needed
required two Google searches, both of which might be of use to you along
with the one finding that was close to what I expected.
I recalled a paper in which SEM was performed on Ab-stabilized, then
fixed, brush border. I couldn't remember that so I went searching - not
for you, for me. I found:
http://www.uth.tmc.edu/apstracts/1995/lung/December/226l.html. My two
Google searches were: "glycocalyx fixation"; and "glycocalyx antibody"

Good luck,

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Fri Nov 2 09:49:12 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 02 Nov 2001 10:41:32 -0500
Subject: RE: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paula,

I'm usually wary when someone is fixated on a particular
"magic" number, 30 or 300 or whatever. Often you are
dealing with a person who doesn't understand statistics
nearly as well as they think they do. Since you haven't
been able to convince her of your position, maybe you can
ask her to justify hers? Make her really explain (and
hopefully understand) where that 30-40 target comes from.
I don't necessarily *recommend* this approach, since it
may lead to more conflict than collaboration!

I don't know that a particular reference like the one you
seek is available. As others have pointed out, it's hard
to give specific advice without more specifics of your
experiments. General suggestions might include 1) showing
her other "comparable" microscopy examples from the
literature; 2) explaining the total cost per sample in
dollars and/or person-hours; 3) promising to include
standard disclaimers or a brief discussion about the
statistical relevance of the microscopy portion. I suspect
you have already tried most or all of these. So lastly,
about the only specific example I can think of is the
various asbestos analysis regulations that attempt to apply
statistics to microscopy (particle counting). There is a
long history of trying to establish a minimum
"representative" sub-sample based on sample heterogeneity.
Feel free to email me if you think any of that may be
helpful. Good luck!

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Thursday, November 01, 2001 9:41 AM, Paula Allan-Wojtas
[SMTP:AllanWojtasP-at-EM.AGR.CA] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
} ------------------------------------------------------
----
} -------------.
}
}
} Hi, all,
}
} I am working on a multidisciplinary team of researchers
} on a number of very interesting projects. I have a very
} large collection of top quality micrographs from these
} projects which I would like to publish. The microscopy
} samples have been selected and photographed within the
} experimental design which has been set out for each
} project, so we are pretty sure they are representative of
} what's there.
}
} One collaborator on the team is hesitant (to the point of
} being resistant) about my publishing any of the
} microscopy as part of any of the publications put
} together by the team because we have not looked at 30-40
} samples of each of the many experimental treatments ( we
} have done 4 samples of each treatment) . The other
} researchers on the team have looked at between 30 and 40
} samples per treatment. This one collaborator says that
} she has no confidence in the microscopy results because
} "the statistics just aren't there".
}
} I have tried on numberous occasions to explain to her
} about why this is not feasible to do in SEM, and is
} usually not done. She does not believe me and is
} essentially preventing me from publishing the work. I
} could probably publish this work as a stand alone ms, but
} it is so much more meaningful when integrated with data
} from the other analytical techniques.
}
} My question: is there a reference (which I have somehow
} missed) to explain why 30-40 samples is not feasible for
} microscopy and which rationalizes why this is so? The
} function of microscopy in the projects has been to give
} pictorial information about the samples, and to suggest
} trends, not for absolute measurement of structures and
} changes in them.
}
} Any help you can provide would be very much appreciated
-
} before I rip all of my hair out....
}
} Thanks in advance, listers, to all of you. I know I came
} to the right place.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca



From daemon Fri Nov 2 10:09:25 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 2 Nov 2001 07:51:07 -0800
Subject: Re: Ask-A-Microscopist: oculars for use by visually impaired

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Email: griggsa-at-dbcc.cc.fl.us
} Name: Alvin Griggs
}
} Education: Undergraduate College
}
} Location: Daytona Beach, FL, USA
}
} Question: I am looking for places that sell or lease microscope
} projection oculars for use by visually impaired students.
}
} ---------------------------------------------------------------------------
Alvin -

I would like to suggest that the current selection of add-on video cameras
or direct digital input to a PC is a better way to go. You don't describe
your application or budget, but simple video can be as cheap as $300.
You'll find good descriptions of some possibilities at
www.microscopeworld.com, but there are MANY sources.



Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Nov 2 12:32:05 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Fri, 02 Nov 2001 13:02:38 -0500
Subject: Food Structure and Functionality Symposium 2002 - Second

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Second Announcement

Food Structure & Functionality Symposium 2002

May 5 to 8, 2002, Palais des Congres de Montreal, Montreal, Quebec, Canada

held in conjunction with the 93nd AOCS Annual Meeting & Expo (www.aocs.org)

webaddress at the AOCS site: http://www.aocs.org/member/division/fsff/index.htm

Food Structure and Functionality Forum Bulletin Board:
http://www.aocs.org/ubbcgi/ultimatebb.cgi

Tentative Technical Program Schedule (as of November 2nd, 2001)

Sunday, May 5th
Short Course - Understanding structure-function relationships in food systems through specific
localisation methods and microscopy. Contact: Marcel Paques (Paques-at-nizo.nl)
---------------------------------------------------------------------------------------------------------------
Monday, May 6th-Morning
Opening of symposium - Opening remarks

Plenary Speaker and presentation of Division Achievement Award

Dairy Applications Session. Chairs: Mark Auty, Dairy Products Research Centre, TEAGASC, Ireland (mauty-at-moorepark.teagasc.ie ) and Harjinder Singh, Massey University, NZ (H.Singh-at-massey.ac.nz)

Some Observations of a Microscopist, Author, and Reviewer on Structural Studies of Milk Products. M. Kalab, Agriculture and Agri-Food Canada, Canada

Effect of Emulsifiers and Processing Conditions on Microstructure of Milk Fat/Sunflower Oil Blends. S. Martini1, M. Cerdeira1, C. Puppo1, R.W. Hartel2, and M.L. Herrera1,3, 1CIDCA, UNLP, CONICET, Argentina; 1University of Wisconsin-Madison, USA; 3University of Buenos Aires, Argentina

Texturization of Dairy-Based Spreads. Y. Shi, B. Liang, and R.W. Hartel, University of Wisconsin-Madison, USA

Localization of Whey and Casein in Cheeses Using Microscopy and Immunochemistry Techniques. Y. Wang and D. Pechak, Kraft Foods, U.S.A

Manufacturing Yoghurt Structures with a Predicted Consumer Preference. M Langton and A. Astrom, SIK, Sweden

Dynamic Confocal Imaging of Tension and Fracture in Composite Food Materials. D.P Ferdinando1, K.P Plucknett2, and V. Normand3, 1Unilever Research, UK; 2DERA, UK; 3Firmenich SA, Switzerland

TBA - Topic: Dairy powders/caramels. C. Attapattu, University of Wisconsin, USA

Monday, May 6th - Afternoon
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl ) and David Pechak (Dpechak-at-kraft.com)

Protein Polysaccharide Interactions. C.G. De Kruif, NIZO Food Research, Netherlands (keynote speaker)

To Be Announced. B. Campbell, Kraft Foods, USA

Structure in Heat Treated Low_Fat Emulsions. R. Ofstad and V. Hoest, MATFORSK, Norway

Fatty Acid Salts-Induced Gels of Food Proteins: Their Rheological Properties and Structural Changes of the Proteins During Gelation. N. Yuno-Ohta, Nihon University, Japan

Wheat Gluten Proteins. A.S. Tatham, IACR Long Ashton research Station, United Kingdom

Interfacial Composition and Stability of Oil in Water Emulsion formed with Mixtures of Milk Proteins and Polysaccharides. H. Singh and Y. Hemar, Institute of Food, Nutrition and Human Health, Massey University, NZ

Dedicated Poster Session

Division Board Meeting
--------------------------------------------------------------------------------------------------------------------
Tuesday, May 7th - Morning
Agricultural Applications of Microscopy and Imaging Session/ joint with Feed Microscopy Division. Topic: Food Contamination

contacts: Mark Auty, Dairy Products Research Centre, TEAGASC (mauty-at-moorepark.teagasc.ie ) and Marge McCutcheon, West Virginia Department of Agriculture, USA (Feed Microscopy Division)

Forensic tampering. F. Platek, FDA (keynote speaker)

Contaminants in Food Processing. D. Kittleson, Pillsbury

How to approach contaminant identification. M. Auty, Dairy Products Research Centre

Growth promoters. P. Klink, South Africa

Identification of plant material. D.F. Wood, USDA

To Be Announced. J. Makowski

Quanitation of Total Fat and Fat Quality in Cheese and Dairy Products Using Membrane Separation Technology. V.C. Gordon, Safety Associates, Inc., USA

Additonal speakers to be announced.

Division Luncheon and round table (expert) discussion. Topic to be announced

Tuesday, May 7th - Afternoon
Microbiology and Food Session Chairpersons: Judy Arnold and Ida Yates, USDA, ARS, Russell Research Center, USA

Prevention of Bacterial Fouling on Food Equipment Surfaces. J.W. Arnold, USDA, ARS, Russell Research Center, USA

Probiotics and Their Use in Food Animal Production. R. Droleskey, USDA, ARS, SPARC, USA

The Effect of High Pressure Steriliztion on Listeria Inoculated Seafood. K.R.S. Schneider and M.V.W. Wood, University of Florida, USA

Food Microstructure Investigations by Atomic Force Microscopy. J. Thornton, Digital Instruments/Veeco Metrology Group, USA

Controlling Growth of the Toxigenic Fungus, Fusarium Verticillioides. I. Yates and J. Arnold, Russell Research Center, ARS, USDA, USA

Division Members Meeting (immediately following the afternoon session)
--------------------------------------------------------------------------------------------------------------------
Wednesday, May 8th- Morning
Ingredients and Food Processing Session- Chairpersons: Diana Kittleson, Pillsbury Co, TPC Labs, USA; and Bernhard Tauscher, Federal Research Center for Nutrition, Germany

Non-Invasive Quality Determination of Fruit and Vegetables: Application of a Multi-Wavelength NIR-Diode Laser Array. B. Tauscher, Federal Research Center for Nutrition, Germany

Characterisation of the Application of Novel Oil Structurants. E. Floeter, F. Gandolfo, and W. Hogervorst, Unilever Research Vlaardingen, The Netherlands

High Pressure Processing. E. Ting and E. Raghubeer, Flow International, USA

Microstructure of Rice Starch Isolates. D.F. Wood1, A.M. Ibanez_Carranza2, and C.F. Shoemaker2, 1USDA, ARS, WRRC, USA; 2University of California, USA

To Be Announced. F. Escher, B. Conde-Petit, ETH, Switzerland

To Be Announced. M. Michel, Nestec Ltd., Nestle Research Center, Switzerland

To Be Announced. M. Salmenkallio-Marttila , VTT Biotechnology, Finland

Wednesday, May 8th - Afternoon
New Methods and Techniques for Food Structure and Functionality Analysis Session
Chairpersons: Kathy Groves, Leatherhead Food Research Association, England; and Maud Langton, SIK, Sweden

Quantifying Microstructures through Image Analysis. G.M.P. van Kempen1, M. van Ginkel2, C.L. Luengo Hendriks2, L.J. van Vliet2, and S. Singleton3, 1Unilever Research
Vlaardingen, Netherlands; 2Delft University of Technology, The Netherlands; 3Unilever Research Colworth House, Great Britain

Cryo-TEM of Biopolymers in Comparision with Other TEM-techniques. M. Langton, A. Altskar, and A.-M Hermansson, SIK, Sweden

Freeze-substitution and low temperature embedding of dairy products for electron microscopy.
A.K. Smith and H.D. Goff, Department of Food Science, University of Guelph, Canada

Recent Advances in our Understanding of the Relationship Between Crystallization Behavior, Microstructure and Rheological Properties of Fat Crystal Networks. A. Marangoni, University of Guelph, Canada

Spectroscopic Prediction of Rheological Properties in Grains. F. Meadows and F. Barton USDA, ARS, QARU, USA

Staining Techniques for Detection of Components in Fish Muscle. K. Hanneson Eggen and G. Enersen, Matforsk, Norway

Closure of Symposium
----------------------------------------------------------------------------------------------------------------------
Posters

Relationships between Microstructure and Rheological Properties of Model Lipid Systems. B. Liang, Y. Shi, and R.W., Hartel Univ. of Wisconsin-Madison, USA

Minor Biomolecules from the Olive Drupe to Olive Oil: The Technology and the Well-being Effects. N. Uccella, CIRASAIA-Mediterranean Agrifood Research Centre, Calabria University, Italy

Formation and Physical Properties of ?-Fat Gel. I: Macroscopic and Microscopic Observations. K. Higaki1, Y. Sasakura1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. Ii: In situ Observation of Gel-Formation Processes. K. Higaki1, Y. Sasakura1, I. Hachiya1, S. Ueno2, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. III: Rheological Properties. K. Higaki1, T. Koyano1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. IV: Why and How? K. Sato1, K. Higaki2, T. Koyano2, and I. Hachiya2, 1Faculty of Applied Biological Science, Hiroshima University, Japan; 2Meiji Seika Kaisha Ltd., Japan



From daemon Fri Nov 2 12:38:58 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 02 Nov 2001 10:38:32 -0800
Subject: Re: SEM - how many samples are enough?

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} Date: Fri, 02 Nov 2001 07:40:47 -0800
} To: "Paula Allan-Wojtas" {AllanWojtasP-at-EM.AGR.CA}
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: SEM - how many samples are enough?
}
} Coming from a semiconductor background, I have a
} somewhat different take on this topic.
}
} In evaluating the quality of IC wafer runs, there are
} two methods I use. One way is to check each wafer
} while the other is to check only one wafer. Either
} way, only four die off of the wafer are examined via
} SEM. This is in accordance with MIL-STD-883 Method
} 2018, which can be found at:
}
} http://www.dscc.dla.mil/Downloads/MilSpec/Docs/MIL-STD-883/std883_2018.pdf
}
} In particular, look at paragraph 3.1.1 and Figure 2018-4.
}
} This area discusses die sampling procedures for wafer lots.
} Considering that one wafer may contain upwards of 5,000 die
} (depending on wafer size and die size), examination of
} all die, or even a large number of die is unrealistic. The issue
} is whether the sample is representative of the rest of the wafers
} and the die therein. There are indeed strange things that can
} and do happen to wafers as they are processed such that
} the results of examining a randomly-pulled wafer may not
} be correct about the whole wafer lot--or some of the other
} wafers in the lot.
}
} If there is a reasonable expectation of repeatability from
} wafer-to-wafer, then examination of one wafer and the
} prescribed number of die will pass or fail the whole wafer
} lot. perhaps this same reasoning might apply to your
} situation? I certainly don't SEM 40,000 die! There are
} in-process control wafers which are examined during
} the course of fabrication. Failure at any of these steps
} fails the whole wafer. But the small sample at the end
} of fabrication is a pas/fail for the whole wafer lot.
}
} If you were to be examining E. coli for example and
} imaged say ten individuals, and all were rod-shaped.
} How many more specimens would you have to image
} to find one that was round? The issue seems to me
} to be more of the characteristics of the specimens
} being examined. 100% coverage will always be
} someone's desired approach. But of course, this
} is not practical nor realistic for most situations. Thus,
} the sampling criteria is applied. Which one is
} applied is the key issue. How much or how many
} is sufficient to draw a conclusion?
}
} gary g.
}
}
} At 06:41 AM 11/1/2001, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Nov 2 13:46:25 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Fri, 2 Nov 2001 14:30:25 -0500
Subject: Re: Help for freeze substitution protocols

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Hi Christopher,

We did a lot of freeze substitutions of plant leaf tissues, but with
fixatives. However, I do believe the principle would be the same though
without fixatives you would not clearly see the ultrastructure of the
cells.
Following are some references you might like to check.

Xu and Mendgen (1994) Planta 195:282
Xu and Mendgen (1997) MPMI 10:87

and some publications in Dr. K. Mendgen's lab.

Hope it helps.

Haixin Xu (Ph D)

Biological Sicences
University od Maryland Baltimore County
1000 Hilltop Circle
Baltimore, MD 21250

On Thu, 1 Nov 2001,
Christopher Ogomo wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Greetings fellow listers.
}
} I'm searching for freeze-substitution protocols (without using of any
} fixative), preferably fast and slow protocols, for plant leaf specimen
} preparation. I'm working on localizing nickel element in the plant leaves of
} a hyperacumulator plant, hence using cryo preparation methods. I'll highly
} appreciate any contributions and help in this.
}
} My thanks in advance.
}
} Chris Ogomo
} MSc Student
} University of the Western Cape
} Rep of S. Africa
}
}
}
}
}
}
}
}



From daemon Fri Nov 2 13:47:51 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Fri, 2 Nov 2001 14:42:13 -0500 (EST)
Subject: thanks for protocols

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Thanks to all the generous folks who sent me their microspherule protocols
- The info is most appreciated!
Dee



***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Fri Nov 2 14:16:54 2001



From: Edward_Principe-at-amat.com
Date: Fri, 2 Nov 2001 12:10:12 -0800
Subject: Re: SEM - how many samples are enough?

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I think this is a very interesting thread. I agree with most of the other
comments contributed. It is the sort of problem that is not always clear
but always important. In certain situations there are clear quantitative
ways to arrive at the number of samples (measurements) required for a given
confidence interval (key term), of say 95%, applied to a t-distribution
assumption (usually). This type of formula can be plucked directly from
statistical texts and there are even nifty little sample size calculators
on the web. But, it is not always obvious how to apply that to a given
measurement, and as others have said, we don't know exactly what you are
trying to quantify. Within a given type of measurement, there are also
ways to establish the precision of a given measurement method and then
knowing that value, apply it to a single independent measurement acquired
under conditions your accuracy/precision estimate was valid. There are
very commonly scenarios where your final calculation also has errors that
are cumulative and generally these add in quadrature. So, say you measure
something and then calculate a result that involves ratios of this
intensity or that, and/or multiplication of one length with another to get
your final answer. Errors associated with division, multiplication and
exponents are also known. In related schemes, say your calculation
involves some intensity measurement and you know your the level of "noise"
in that intensity measurement with a given RMS level, then that error may,
in the most simple approximation, be given as 3X the rms and however that
may be related to your final results would possibly be part of the
quadrature-based calculation of your total error. I guess my point is
there should be a way to estimate error within a given confidence interval
in a single measurement if you know what the errors involved in the
fundamental measurement, be it intensity, distance, etc. But, you need to
break down that calculation and pick out the individual errors to determine
the final error. Accuracy, versus precision, is a distinction I am sure
you can appreciate.

I should say, I am definitely not a statistical expert but like you I have
had to deal with this situation on several occaisons, as I am sure others
have. Let's face it, it is also more work to generate error estimates for
everything we do. Given the option we'd all like to plot and quantify with
error bars and confidence limits, it is just not always clear how to "get
there from here". My overall opinion is it is quite likely you have a
valuable contribution and you need to determine some way to express
qualitatively the limit in the interpretable information from the image
(assuming it is an image) or quantitatively estimate that will satisfy your
collegues. Good luck and please share any enlightment you might find as a
results of this quest!

Regards,
Ed Principe


} Paula Allan-Wojtas wrote:
}
}
} --------------------------------------------------------------------
----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------
---.
} }
} } Hi, all,
} }
} } I am working on a multidisciplinary team of researchers on a
number of very interesting projects. I have a very large collection of
top quality micrographs from these projects which I would like to
publish. The microscopy samples have been selected and photographed
within the experimental design which has been set out for each
project, so we are pretty sure they are representative of what's
there.
} }
} } One collaborator on the team is hesitant (to the point of being
resistant) about my publishing any of the microscopy as part of any of
the publications put together by the team because we have not looked
at 30-40 samples of each of the many experimental treatments ( we have
done 4 samples of each treatment) . The other researchers on the team
have looked at between 30 and 40 samples per treatment. This one
collaborator says that she has no confidence in the microscopy results
because "the statistics just aren't there".
} }
} } I have tried on numberous occasions to explain to her about why
this is not feasible to do in SEM, and is usually not done. She does
not believe me and is essentially preventing me from publishing the
work. I could probably publish this work as a stand alone ms, but it
is so much more meaningful when integrated with data from the other
analytical techniques.
} }
} } My question: is there a reference (which I have somehow missed) to
explain why 30-40 samples is not feasible for microscopy and which
rationalizes why this is so? The function of microscopy in the
projects has been to give pictorial information about the samples, and
to suggest trends, not for absolute measurement of structures and
changes in them.
} }
} } Any help you can provide would be very much appreciated - before
I rip all of my hair out....
} }
} } Thanks in advance, listers, to all of you. I know I came to the
right place.
} }
} } Paula.
} }
} } Paula Allan-Wojtas
} } Research Scientist - Food Microstructure
} } Agriculture and Agri-Food Canada
} } Atlantic Food and Horticulture Research Centre
} } Kentville, Nova Scotia Canada B4N 1J5
} }
} } Tel: (902) 679-5566
} } FAX: (902) 679-2311
} }
} } email: allanwojtasp-at-em.agr.ca







From daemon Fri Nov 2 15:46:36 2001



From: Russell McConnell :      russell.mcconnell-at-tufts.edu
Date: Fri, 02 Nov 2001 16:37:57 -0500
Subject: ER staining

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Hi listers,
I am looking for an ER specific fluorophore that can be excited using
visible wavelength lasers. The cells that this would be used in are
cultured fibroblasts. If possible, either the excitation or emission
(one or the other, it needn't be both) would be different from those of
Cy3 and Cy5. If anyone knows of any good kits available for this, I
would greatly appreciate it (Mol. probes has a kit, but it requires UV
excitation, a laser that I unfortunately do not have).

Thanks in advance,
Russell
--
Russell McConnell
Confocal Imaging Facility Technician
Department of Neuroscience
Tufts University School of Medicine
M&V Building room #137
136 Harrison Ave.
Boston, MA 02111
Tel. (617) 636-3795


From daemon Fri Nov 2 17:19:17 2001



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Sat, 3 Nov 2001 10:23:05 -0330
Subject: RE: SEM - how many samples are enough?

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Doug:

I am forwarding the email from van Heel (sent to 3dem). It is a good
summary of scheduling/booking softwares.
I personally like iCal (http://www.brownbearsw.com/). Hope it'll
help--Zhaojie Zhang


**************************************
Zhaojie Zhang, Ph.D.
Manager of Microscopy Facility
University of Wyoming
http://www.uwyo.edu/microscopy
**************************************

-----Original Message-----
} From: van Heel, Secretary T P M [mailto:vanheel.office-at-ic.ac.uk]
Sent: Thursday, October 25, 2001 5:36 AM
To: '3dem-at-sdsc.edu'


Paula writes ...

} ... I have a very large
} collection of top quality micrographs from these projects which I
} would like to publish. The microscopy samples have been selected
} and photographed within the experimental design which has been
} set out for each project, so we are pretty sure they are
} representative of what's there.
}
} One collaborator on the team is hesitant (to the point of being
} resistant) about my publishing any of the microscopy as part of
} any of the publications put together by the team because we have
} not looked at 30-40 samples of each of the many experimental
} treatments ( we have done 4 samples of each treatment) . The
} other researchers on the team have looked at between 30 and 40
} samples per treatment. This one collaborator says that she has no
} confidence in the microscopy results because "the statistics just
} aren't there".
} ...

As others have implied ... how many samples depends on the variation you
see. If you had 3 samples and absolutely no variation, you may be justified
in stopping there and drawing a conclusion ... however, this is generally
never the case. Statistical tools, such as the "chi-square distribution",
are sometimes used for drawing conclusions from a small set, but even
generalized chi-square formulas don't begin to yield meaningful conclusions
unless the # of samples is ~30. This "rule-of-thumb" is probably at the
root of your colleagues criticisms ... as if we remembered anything from
"statistics 101".

shAf :o)



From daemon Sat Nov 3 09:59:37 2001



From: John Bonevich :      john.bonevich-at-nist.gov
Date: Sat, 3 Nov 2001 10:45:19 -0500
Subject: Post Doctoral Positions

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The National Institute of Standards & Technology (NIST) and the
Materials Science and Engineering Laboratory have post-doctoral
positions open. These positions are offered competitively through
the National Research Council (NRC). The Materials Structure and
Characterization Group at NIST has many scientists utilizing our
research facilities including TEMs (HRTEM, S/TEM, EELS, EDS) and SEMs
(FEG, LaB6 equipped with OIM/EBSD).

We employ microscopy techniques to fundamental and challenging
problems in materials science, semiconductors and optoelectronics.
Recent topics have included semiconductor interconnections, nanometer
scale multilayered materials, quantum dot materials, spintronic
devices, etc.

The NIST/NRC Post Doc program offers a two-year position at an annual
salary of approximately $53,200 with an additional $5,500 for
research expenses. The applications are due to the NRC by Jan 15,
2002. The application must include a brief proposal and several
recommendations.

A candidate must be a U.S. citizen and start work (with their PhD in
hand) at NIST between July 2002 and January 2003. Students
graduating this spring through next fall and others that have
received their degree within the last five years are encouraged to
apply. The NIST/NRC Post Doctoral program only selects positions
once a year, unlike some other institutions.

You may contact me or visit our web site for further information at

{http://www.metallurgy.nist.gov/opportunities.html}

--

--------------------------
John Bonevich, Ph.D.
NIST, Metallurgy, Stop 8554
100 Bureau Drive
Gaithersburg, MD 20899 USA
TEL: (301) 975-5428
FAX: (301) 975-4553


From daemon Sat Nov 3 16:52:01 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: Sat, 3 Nov 2001 14:32:54 -0800
Subject: LM/EM Autoradiography

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Hello,

Is there anyone who still uses autoradiography as a routine technique?

I am looking for suppliers of film and emulsion for both EM and LM autoradiography and wonder what is still available.

Any methods, tips, tricks or special needs (light filters, methods for preparing solutions etc) are especially welcome.

Regards,

Paul Webster




From daemon Sun Nov 4 00:15:18 2001



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: Sat, 3 Nov 2001 21:53:45 -0800
Subject: SEM: How many samples are enough?

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Paula writes ...
"I have a very large collection of top quality micrographs from these projects which I would like to publish. The microscopy samples have been selected and photographed within the experimental design which has been set out for each project, so we are pretty sure they are representative of what's there.

One collaborator on the team is hesitant (to the point of being resistant) about my publishing any of the microscopy as part of any of the publications put together by the team because we have not looked at 30-40 samples of each of the many experimental treatments ( we have done 4 samples of each treatment). The other researchers on the team have looked at between 30 and 40 samples per treatment. This one collaborator says that she has no confidence in the microscopy results because "the statistics just aren't there". "

} From Paul Webster:
I am getting into this subject a little late so forgive me if I repeat something that has already been said.

The answer to the question, as posted by Michael S. a little earlier, is "... how many samples depends on the variation you see." This is exactly the solution to the problem. However, to get to a meaningful answer requires us to know what is the variation within the population. This may take a little more experimental planning. This point has been taken up in the rest of Michaels answer and also in previous replies concerning the examination of homogeneous materials samples. Homogeneous sample populations will require less sampling that heterogeneous populations.

To get a "representative" estimate of normal human blood temperature should be pretty easy as we all hover around the same temperature. Of course, a bias will be introduced if we include hospital patients in our sample. This is only a bias if we want an estimate for healthy individuals.

Conversly, obtaining an estimate for the mean height of a population will depend on who is being measured and their location. There will be many different, and correct answers for this one. Of course, the limitations of the estimation should be included with the final estimate (e.g. estimated mean height of adult males, age 35, attending a Yankees baseball game). Hwo many males should be measured so that we obtain a meaningful result? This will depend on the total population, their habits, and the sampling protocol applied. Selecting males going to the game who are seen coming out of "Big and Tall" stores may not be a representative sample!

Representative images in microscopy, selected to be "representative" are actually biased samples in that they have been selected by the operator. To get a truly "representative" result implies that unbiased estimators must be applied to the samples under investigation. An unbiased estimation should then be in the form of a quantitative result and not a "representative" image.

There are many powerful, efficient and easily applied protocols available for estimating number, length, surface area and volume. However, to be applied correctly, they must be applied to truly representative samples of the population. To obtain this unbiased sample requires the application of unbiased sample collection.

I saw in an earlier posting the cautious warning not to confuse accuracy with precision. This is an important warning. It is easy to be precise and still get the wrong answer. Imagine, for example, the result of a target practice using a gun with crooked sights but in the hands of an exceptional marksman. The holes will probably all be clustered together. The bias due to the crooked sight may cause the holes to be way of center. However, in the hands of a novice, the gun will fire off-target, but the operator may possibly be so inaccurate that a stray shot will hit the bulls-eye.

In the words of the sterologists "do more - less well". In biology, most of the variation occurs at the level of the cell or the animal. There will be less variation between samples taken from the same animal or cell population. It is far more reliable to measure small samples from more animals or cells than to take lots of images from one sample.

There are many unbiased sampling protocols available to use. However, most of them are designed to be applied to thin sectioning. It is possible that they can be adapted for use in the SEM. Estimating number and particle size should be possible using ramdom fields.

To obtain more information on these protocols I suggest looking up papers by John Lucocq and Terry Mayhew, who have both written very readable reviews of the challenges of random sampling. In particular, look for descriptions of Systematic Random Sampling.
You will also see references to uniform isotropic sampling to eliminate bias.

You may come to the conclusion that taking one or two random images from your 30-40 samples, and then estimating the paramenters of interest from them is the way to go.

Microscopy has become a powerfully quantitiative branch of science. As such it is possible to express the qualitative results we see every day in unambiguous ways. We should make use of these tools to remove the fuel for argument.

Paul Webster.


From daemon Sun Nov 4 12:53:20 2001



From: zaluzec-at-microscopy.com
Date: Sun, 4 Nov 2001 12:32:49 -0600
Subject: Administrivia: 100,000,000 and counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listserverites...

In setting up the monthly archives for October of 2001
I got moderatley curious about the number of Email messages/ deliveries
have run through the system running out of my home/office
these past 8 years (Oct 1, 1993 was the Listserver's start date).

According to my extremely precise calculations ;-) the 100 Millionth Email
message was delivered to a Microscopy Listserver subscriber
sometime this year.

In case your curious, there have been 58, 280 postings to the Microscopy
Listserver over the last 8 years, all of which are stored in the
searchable archives. The subscriber base varies (daily), but as of this
morning we had 2562 subscribers from at least 62 countries on 6
Continents. There
is no one from Antarctica that I can find, or shall we say that I know about,
nor is anyone from the International Space Station subscribed. :-(
We did have at least one person subscribed whose mail is being
forwarded to an Oceanographic survey vessel via a satellite link.

Not counted in the above totals were 6415 "suspect" messages caught
by the Email filter
and were rejected. Just under 3000 of these had attachments, 700+
had forged IP addresses, another ~2000ish were offers for
credit/mortages/loans/viagra/porn etc... but at the same time
number of valid postings { 3 % were inadvertently caught and these are
generally passed through after I get contacted by the authors asking
what is wrong.

I also noticed that the very first subscriber to the Listserver
(Arthur Day from ANSTO just outside of Sydney Australia)
is still receiving his daily dose of Microscopy Email. Well done, Arthur!

Now if I only had a beer for each of those emails, I'd be set for life.

Cheers...

Nestor
Your Friendly Neighborhood SysOp


From daemon Sun Nov 4 15:09:54 2001



From: Larry Allard :      l2a-at-ornl.gov
Date: Sun, 04 Nov 2001 15:59:47 -0500
Subject: Re: Administrivia: 100,000,000 and counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor states:

} } Now if I only had a beer for each of those emails, I'd be set for life. { {

Actually Nestor, by my extremely precise calculations, someone should
buy you your 100,000,000th beer about MSA time next year...something
to look forward to in Quebec City!

Larry :-)




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listserverites...
}
} In setting up the monthly archives for October of 2001
} I got moderatley curious about the number of Email messages/ deliveries
} have run through the system running out of my home/office
} these past 8 years (Oct 1, 1993 was the Listserver's start date).
}
} According to my extremely precise calculations ;-) the 100 Millionth Email
} message was delivered to a Microscopy Listserver subscriber
} sometime this year.
}
} In case your curious, there have been 58, 280 postings to the Microscopy
} Listserver over the last 8 years, all of which are stored in the
} searchable archives. The subscriber base varies (daily), but as of this
} morning we had 2562 subscribers from at least 62 countries on 6
} Continents. There
} is no one from Antarctica that I can find, or shall we say that I know about,
} nor is anyone from the International Space Station subscribed. :-(
} We did have at least one person subscribed whose mail is being
} forwarded to an Oceanographic survey vessel via a satellite link.
}
} Not counted in the above totals were 6415 "suspect" messages caught
} by the Email filter
} and were rejected. Just under 3000 of these had attachments, 700+
} had forged IP addresses, another ~2000ish were offers for
} credit/mortages/loans/viagra/porn etc... but at the same time
} number of valid postings { 3 % were inadvertently caught and these are
} generally passed through after I get contacted by the authors asking
} what is wrong.
}
} I also noticed that the very first subscriber to the Listserver
} (Arthur Day from ANSTO just outside of Sydney Australia)
} is still receiving his daily dose of Microscopy Email. Well done, Arthur!
}
} Now if I only had a beer for each of those emails, I'd be set for life.
}
} Cheers...
}
} Nestor
} Your Friendly Neighborhood SysOp

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Sun Nov 4 16:27:09 2001



From: Mark G BLACKFORD :      mgb-at-ansto.gov.au
Date: Mon, 5 Nov 2001 09:18:20 +1100
Subject: RE: Administrivia: 100,000,000 and counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

for symmetry I think Arthur Day should be the one to buy Nestor his
100,000,000th beer, and the 17th Australian Conference on Electron
Microscopy in Adelaide this coming February would be the perfect place.
Adelaide is quite warm that time of year , perfect beer drinking conditions.
Why don't all you listservers come and join us. Cheers,


Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.


} ----------
} From: Larry Allard
} Sent: Monday, November 5, 2001 7:59 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Administrivia: 100,000,000 and counting
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Nestor states:
}
} } } Now if I only had a beer for each of those emails, I'd be set for
} life. { {
}
} Actually Nestor, by my extremely precise calculations, someone should
} buy you your 100,000,000th beer about MSA time next year...something
} to look forward to in Quebec City!
}
} Larry :-)
}
}
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Listserverites...
} }
} } In setting up the monthly archives for October of 2001
} } I got moderatley curious about the number of Email messages/
} deliveries
} } have run through the system running out of my home/office
} } these past 8 years (Oct 1, 1993 was the Listserver's start date).
} }
} } According to my extremely precise calculations ;-) the 100 Millionth
} Email
} } message was delivered to a Microscopy Listserver subscriber
} } sometime this year.
} }
} } In case your curious, there have been 58, 280 postings to the
} Microscopy
} } Listserver over the last 8 years, all of which are stored in the
} } searchable archives. The subscriber base varies (daily), but as of this
} } morning we had 2562 subscribers from at least 62 countries on 6
} } Continents. There
} } is no one from Antarctica that I can find, or shall we say that I know
} about,
} } nor is anyone from the International Space Station subscribed. :-(
} } We did have at least one person subscribed whose mail is being
} } forwarded to an Oceanographic survey vessel via a satellite link.
} }
} } Not counted in the above totals were 6415 "suspect" messages caught
} } by the Email filter
} } and were rejected. Just under 3000 of these had attachments, 700+
} } had forged IP addresses, another ~2000ish were offers for
} } credit/mortages/loans/viagra/porn etc... but at the same time
} } number of valid postings { 3 % were inadvertently caught and these are
} } generally passed through after I get contacted by the authors asking
} } what is wrong.
} }
} } I also noticed that the very first subscriber to the Listserver
} } (Arthur Day from ANSTO just outside of Sydney Australia)
} } is still receiving his daily dose of Microscopy Email. Well done,
} Arthur!
} }
} } Now if I only had a beer for each of those emails, I'd be set for life.
} }
} } Cheers...
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
}
} --
} Dr. Lawrence F. Allard
} Distinguished Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov
}
}


From daemon Sun Nov 4 18:58:39 2001



From: Victor Sidorenko :      antron-at-space.ru
Date: Mon, 5 Nov 2001 00:39:23 +0300
Subject: Image plates and CCD in very high voltage TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi friends!

Does anybody have the information about the using possibility of
image recording systems (like PIXsysTEM from JEOL or the
same from Fuji) and CCD cameras (for example from Gatan or
another companies) in 1,000 kV TEM?

Thank you in advance,
Victor Sidorenko, ANTRON Co., Ltd., scientific service,
Moscow, Russia.



From daemon Sun Nov 4 19:03:55 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 5 Nov 2001 13:56:47 GMT+1200
Subject: Franz magnetic separater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello world

Do any of the more geologically-inclined persons have an e-address
for the company that makes the Franz magnetic separator?

thanks

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Nov 5 01:37:11 2001



From: Ian Lamswood :      Ian_Lamswood-at-compuserve.com
Date: Mon, 5 Nov 2001 02:27:14 -0500
Subject: Help for freeze substitution protocols

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Chris,
If you check our new website, em-preparation.com you can
download our manual, 'AFS Recipe book'.
Here you will find a chapter on the freeze substitution of plant
tissue including leaves.
I hope you find it useful.
Regards,

Ian Lamswood
Marketing Manager,
EM Products
Leica, Vienna


From daemon Mon Nov 5 04:31:11 2001



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Mon, 5 Nov 2001 12:53:26 +0100
Subject: RE: scheduling software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Having run a listserv I can appreciate the stable service that Nestor
provides and appreciate the quality assistance the list provides. I have
seen it found many things that I though were made of pure unobtainium with
ease and help the list provides people world wide.

This is the best all around list I follow and I follow a lot. I don't have
a lot to do being retired and not to mobile. I have a broad range of
interests in science and agriculture and there is no question that Nestor
and you members provide the best product out there. Be it a Nobel laureate
or a 6th grader you treat their question with consideration and usualy
present several approaches.

You should be presented a pin at the next meeting and clusters with each
additional billion messages or a pitcher of beer your choice.

} From all of us the appreciate your work

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger


} From: {zaluzec-at-sparc5.microscopy.com}
Sent: Sunday, November 04, 2001 12:32 PM


Hi,

CMP Cientifica today published the first Nanotechnology Overview white
paper, attempting to give a global overview of nanotechnology. Although
written with a business audience in mind, there is probably plenty in there
to interest anyone working at the nanoscale.

The white paper (1.5 Mb) is available for free download at
www.cmp-cientifica.com & other locations.

Any feedback from the microscopy community would be much appreciated, feel
free to contact me directly.

Regards

Tim

Disclaimer: The full version of the Report is a commercial product, but the
White Paper is freely available at no charge.

*****************************************************************
Tim E. Harper CEO
CMP Cientifica s.l.
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/






From daemon Mon Nov 5 08:43:31 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 5 Nov 2001 09:26:42 -0500
Subject: RE: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi Paula,


I'd like to add a suggestion to the one's by Matt listed below:

}
} I'm usually wary when someone is fixated on a particular
} "magic" number, 30 or 300 or whatever. Often you are
} dealing with a person who doesn't understand statistics
} nearly as well as they think they do. Since you haven't
} been able to convince her of your position, maybe you can
} ask her to justify hers? Make her really explain (and
} hopefully understand) where that 30-40 target comes from.
} I don't necessarily *recommend* this approach, since it
} may lead to more conflict than collaboration!...
}
} Matt



} } } } My addition; If this person is so hung up on doing 30-40
} } } } samples, peerhaps you can give him'her instructions n sample prep
} } } } and then tell him'her that s/he should feel free to run up all
} } } } the samples on his/her time and that you would then gladly 'scope
} } } } them. I have found that when people learn by personal
} } } } experience how long it takes to do even a "standard' TEM
} } } } embedding they suddenly readjust their "n" requirement to a more
} } } } realistic goal.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Mon Nov 5 11:21:53 2001



From: Bob Roberts :      bobrobs-at-earthlink.net
Date: Mon, 05 Nov 2001 10:18:14 -0700
Subject: EDS/Bernoulli Disks Revisited

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

A customer of mine has a Kevex Delta II analyzer (circa
1989) equipped with the 8 inch/10 Mb Bernoulli disk drives.
He would like to continue using this media for the time
being but is unable to locate any of these disks for data
storage. Thermo Noran is apparently unable to upgrade his
drives to something more current.

If anyone has these disks laying around, and would like to
sell them, please contact me off-line. Thank you.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
480.967.3946
"Home of the 2001 World Series Champion Arizona
Diamondbacks"



From daemon Mon Nov 5 19:14:37 2001



From: Edward_Principe-at-amat.com
Date: Mon, 5 Nov 2001 16:59:37 -0800
Subject: Beer fund

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I say we all mail Nester one beer, something regional or unique. Just
picture the scene :-)


-Ed



From daemon Mon Nov 5 20:48:48 2001



From: chris :      chris-at-worldwafer.com
Date: Mon, 5 Nov 2001 18:36:49 -0800 (PST)
Subject: New Al2O3, GaAs, GaP, GaSb, GaN, InP, InSb, InAs, SOI & Si...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Visit http://www.worldwafer.com or call
800-713-9375, Fax 888-832-0340 or email chris-at-worldwafer.com

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GaAs Stock (More Available, including 4")
1103SACON 76.2 mm x 635 um (100)SI,undp R} 3x107 EPD {7 x 104 2Pol Epi=09
1104SACON 76.2 mm x 635 um (100)SI,undp R} 2x107 EPD {7x104 2Pol Epi E-J Fl=
ts=09
A0244 20x25mmx381um Zn HB cc=3D1018, (111) 1pol/1etch, EPI, EPD {104=09
AR250E 76.2 mm dia. (100) Semi Insul, 2 x 108 ohm-cm, As-Cut=09
A0432 2=94dia x 450 um (100) N/Te, cc=3D1-5 x 1017, 2pol, EPD {8x104=09
A0437 2=94dia x 450 um (100) P/Zn, cc=3D1-5 x 1017, 2pol, EPD {8x104=09
A0860 76.2 mm x 600 um (100) N/Te cc=3D3 x 1018, 1 Pol, EPI=09
A1291 50.8 mm x 450 um (111) P/Zn, cc=3D1-2 x 1017 EPD:3-5x104 , 2 pol Ep=
i=09
A1397 76.2 mm x 600 um (100) SI, Undp R: 1-2 x 108 EPD {8x104, 2 pol, Epi=09
A1583 76.2 mm x 600 um (111) N/Te, cc} 2 x 1018, EPD {8x104, 1 pol, Epi=09
A1595 76.2 mm x 600 um (111) N/Te, cc} 1 x 1018, EPD {8x104, 1 pol, Epi=09
A1784 50.8 mm x 375 um (111) N/Si, cc} 1 x 1017, EPD {5x104, 1 Pol, Epi=09
L6049 76.2 mm x 600 um (100) P/Zn, cc=3D1-5 x 1017, EPD { 8 x 104, 2 pol,=
Epi=09
L6097 50.8 mm x 400 um (110) SI, Undp R} 1x108, EPD {8x104, 2pol, Epi=09
L6133 50.8 mm x 400 um (111)Ga, SI, Undp R} 1x107, EPD {8x104, 1pol, Epi=09
L6165 50.8 mm x 400 um (100), SI, Undp R} 1x107, EPD {8x104, 2 pol, Epi=09
L6223 50.8 mm x 450 um (100) P/Zn, cc=3D2-4 x 1018, EPD { 8 x 104, 1 pol,=
Epi=09
---------

GaP
L6114 50.8 mm x 300 um (111) N/S, cc: 1 - 5 x 1017 EPD { 2 x 105 2 pol EP=
I=09
L6208 50.8 mm x 300 um (100),N/Undpd,cc { 1 x 1016,EPD { 2 x 105,2Pol EPI=
=09

------------

GaSb
11108SA 2=94 2 Polished=09
1.75=94 (100), P-Type =09
2=94 x 4 mm (100), N/Te, Blanks, R { 1017, EPD { 104, Unpol=09
L5467 50.8 mm x 500 um (100), N/Te, cc =3D 6-8x1017, EPD { 1x103, 1 pol=09
L6116 50.8 mm x 500 um (100) P/Undpd, cc:1-2 x 1016,EPD {5 x 103, 1 Pol Ep=
i=09

------=20
InAs
A0254 50.8 mm x 450 um Zn/P-Type (100) 4o to(111), cc:2x1018, 1 pol/1 etc=
h=09
A0300 50.8 mm x 450 um S/N-Type (100) 4o to(111)A, cc: 1018, 1 pol/1 etch=
=09
A0352 50.8 mm x 450 um Zn/P-Type (100)2o to(110), cc:5-7x1018, 1pol, Epi=09
A0358 50.8 mm x 450 um S/N-Type (100) 4o to(111)A, cc: 3-5x1018, 1 pol=09
A0359 50.8 mm x 450 um S/N-Type (100) 4o to(111)A, cc:3x1018, 1 pol/1 etc=
h=09

------- =09
InSb
L5728 50.8 mm x 450 um (111)A, N/Undpd cc: 4-7x1014, EPD {200, 2 pol, Epi=
=09
L5885 50.8 mm x 450 um (100), N/Te, cc: 3-5x1017, EPD {200, 1 pol, Epi=09
L6207 50.8 mm x 450 um (100), N/Undpd, cc: 4-7x1014, EPD {200, 2 pol, Epi=
=09

--------
=09
InP
50.0 mm x 500 um Prime, SI/Fe,(100),R} 1x107,EPD {5x104,E-J,1pol,Epi=09
50.0 mm x 500 um Test, SI/Fe,(100), R} 1x107,EPD {5x104,E-J, 1pol,Epi=09

InP
Indium Phosphide, LEC
(100) +/- 0.2 degrees Orientation
Semi Insulating, Iron doped
Resistivity } 1 x 10^7 Ohm cm
EPD { 5 x 10^4 cm^-2
Euro-Japanese Flats
1 Side Polished, Back Lapped and Etched
50.0 mm diameter x 500 microns thick

-----------------

InGaAs
Wafer 2"=D8 with InGaAs EPI on SI InP {100} , by MOCVD deposition=20
Substrate: Semi-Insulating InP (eg. InP:Fe), Resistivity: } 1 x 10E7 Ohm =
cm, EDP { 1 x 10E4/cm2)EPI: L
attice matched In/Ga alloy layer of n-type InGaAs Nc } 2 x 10E18/cc (usin=
g Si as dopant) Thickness: 0.5 um (+20%)


Ultra-Thin wafers now in-stock as is Float Zone and Ultra-flat wafers.

We also have fresh stocks of 2"-6" GaAs, GaSb, GaP Germanium, InP, InAs, =
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From daemon Mon Nov 5 20:55:59 2001



From: Arthur Day :      ard-at-ansto.gov.au
Date: Tue, 6 Nov 2001 13:50:17 +1000
Subject: Re: Beer fund

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It would be easier to send Nestor to where the beer is. Less postage
that way...

}
} I say we all mail Nester one beer, something regional or unique. Just
} picture the scene :-)
}
}
} -Ed





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/


From daemon Mon Nov 5 21:05:41 2001



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 05 Nov 2001 20:49:46 -0600
Subject: Re: EDS/Bernoulli Disks Revisited

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'll forward this to the Classic Computer Collector's
mailing list. Someone there certainly will have a lead
on some of these.

- John

At 10:18 AM 11/5/01 -0700, Bob Roberts wrote:
} A customer of mine has a Kevex Delta II analyzer (circa 1989) equipped with the 8 inch/10 Mb Bernoulli disk drives. He would like to continue using this media for the time being but is unable to locate any of these disks for data storage. Thermo Noran is apparently unable to upgrade his drives to something more current.
}
} If anyone has these disks laying around, and would like to sell them, please contact me off-line. Thank you.
}
} Bob Roberts
} EM Lab Services, Inc.
} 2409 S. Rural Rd Suite C
} Tempe, Arizona 85282
} 480.967.3946
} "Home of the 2001 World Series Champion Arizona Diamondbacks"



From daemon Mon Nov 5 22:44:48 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Mon, 5 Nov 2001 22:41:38 -0600
Subject: Re: SEM - how many samples are enough?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
To: {mlynn-at-miami.edu} ; {microscopy-at-sparc5.microscopy.com}
} }
} } Hi Paula,
}
}
} I'd like to add a suggestion to the one's by Matt listed below:
}
} }
} } I'm usually wary when someone is fixated on a particular
} } "magic" number, 30 or 300 or whatever. Often you are
} } dealing with a person who doesn't understand statistics
} } nearly as well as they think they do. Since you haven't
} } been able to convince her of your position, maybe you can
} } ask her to justify hers? Make her really explain (and
} } hopefully understand) where that 30-40 target comes from.
} } I don't necessarily *recommend* this approach, since it
} } may lead to more conflict than collaboration!...
} }
} } Matt
}
}
Have someone that really understands statistics look at the paper. There
is no magic number it all depends on the varibility of the data and what you
are saying about the data.

I doesn't sound like either of you can make a strong case for your position.
At least not one that convinces the other.

Good luck
Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger




From daemon Mon Nov 5 22:44:48 2001



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 06 Nov 2001 16:00:52 +1100
Subject: Re: Beer fund

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "John Foust" {jfoust-at-threedee.com}
To: "Bob Roberts" {bobrobs-at-earthlink.net} ; {Microscopy-at-sparc5.microscopy.com}
Cc: {classiccmp-at-classiccmp.org}
Sent: Monday, November 05, 2001 7:49 PM


I might be willing to share some of my 10 MB Bernoulli cartridges. Since I'll
be holding onto some of the drives, I expect I'll not let them all go.

I do have 20 MB cartridges and drives as well, and those drives read the 10 MB
media, yet write only the 20's. At one time it was convenient to put a
bernoulli box at each of several clients' locations in order simply to move the
cartridges along with me, yet be able to accomplish equivalent useful work at
home, which has always been my primary work location.

regards,

Dick

----- Original Message -----
} From: "John Foust" {jfoust-at-threedee.com}
To: "Bob Roberts" {bobrobs-at-earthlink.net} ; {Microscopy-at-sparc5.microscopy.com}
Cc: {classiccmp-at-classiccmp.org}
Sent: Monday, November 05, 2001 7:49 PM


Maybe..


} } } Arthur Day {ard-at-ansto.gov.au} 11/06/01 02:50PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


It would be easier to send Nestor to where the beer is. Less postage
that way...

}
} I say we all mail Nester one beer, something regional or unique. Just
} picture the scene :-)
}
}
} -Ed





Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www:
http://www.ansto.gov.au/



From daemon Mon Nov 5 23:26:30 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 5 Nov 2001 19:19:31 -1000 (HST)
Subject: Re: Beer fund

Contents Retrieved from Microscopy Listserver Archives
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Tell you what - If all the List subscribers come here I can promise the
Hawaii Visitors Bureau will buy you ALL a beer! And 2,562 for Nestor ...

Mahalo nui loa, Nestor!

Tina

http://www.pbrc.hawaii.edu/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Tue Nov 6 00:52:07 2001



From: Arthur Day :      ard-at-ansto.gov.au
Date: Tue, 6 Nov 2001 17:43:41 +1000
Subject: Re: Beer fund

Contents Retrieved from Microscopy Listserver Archives
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Ok Sally I admit that last message was a bit ambiguous. I'll blame
the Melbourne Cup BBQ for that (beer). So how do I weasel out of it?

Nestor wrote: "Now if I only had a beer for each of those emails,
I'd be set for life."

If nature abhors a vacuum/empty stomach, and if there is no such
thing as one beer, then each of us may have to contribute up to
100,000,000 beers each -- one per message. That's up to 100,000,000
times 2562. So, therefore, it would be easier to send Nestor to each
exotic location to sample the beer in person?

Bit of an extrapolation I suppose.

If anybody needs some tips for their next grant proposal.... ;-(


} Maybe..
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Nov 6 06:21:15 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 6 Nov 2001 09:16:23 -0500
Subject: RE: Beer fund

Contents Retrieved from Microscopy Listserver Archives
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I saw a hand full of this media that VCF East...Did they get sold?
I'm not sure who the seller was...maybe Tom Owad?
-Chandra


-----Original Message-----
} From: owner-classiccmp-at-classiccmp.org
[mailto:owner-classiccmp-at-classiccmp.org] On Behalf Of John Foust
Sent: Monday, November 05, 2001 9:50 PM
To: Bob Roberts; Microscopy-at-sparc5.microscopy.com
Cc: classiccmp-at-classiccmp.org


Having mis-spent my youth at Lehigh University, I suggest we all HAVE a beer
FOR Nestor!

Fred Monson


} ----------
} From: "Edward_Principe-at-amat.com"-at-sparc5.microscopy.com
} Sent: Monday, November 5, 2001 7:59 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Beer fund
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I say we all mail Nester one beer, something regional or unique. Just
} picture the scene :-)
}
}
} -Ed
}
}
}


From daemon Tue Nov 6 08:27:03 2001



From: James Talbot :      james-at-ktgeo.com
Date: Tue, 06 Nov 2001 08:13:48 -0800
Subject: Re: Franz magnetic separater

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Hello Ritchie-

The web page for Franz is http://www.sgfrantz.com/

Sincerely,
James Talbot

K/T GeoServices, Inc.
Bulk and Clay Mineralogy by X-ray Diffraction
(214) 403-6342
web site: http://www.ktgeo.com/



Ritchie Sims wrote:

}
} Hello world
}
} Do any of the more geologically-inclined persons have an e-address
} for the company that makes the Franz magnetic separator?
}
} thanks
}
} rtch
}
} Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Tue Nov 6 08:54:32 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Tue, 6 Nov 2001 10:48:14 -0400
Subject: 100,000,000th message

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Keep up the good work Nestor, as in the immortal words of Buzz Lightyear
"To Infinity And Beyond".

-Dave

ps I guess Buzz is an appropriate (..beer...) reference here.


From daemon Tue Nov 6 10:46:41 2001



From: Scott Johnson :      sjohnson-at-brookdale.cc.nj.us
Date: Tue, 06 Nov 2001 11:36:21 -0700
Subject: Re: bacillus anthracis

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AIDS isn't a virus either. More semantics.


} Kristen Lennon wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Semantics because this mistake has been making me crazy - ANTHRAX IS A
} } BACTERIUM! This is a particularly important point because the word "virus"
} } scares people silly. When I think of virus, I think of AIDS and Ebolla
} } (sorry it that's misspelled). When I think of bacterium, I think
antibiotic.
} } No offense intended,
} } Kristen
} }
} } Kristen A. Lennon, Ph.D.
} } Department of Plant Pathology
} } 351 Bessey Hall
} } Iowa State University
} } Ames, IA 50011
} }
} } 515-294-8854
} } kalen-at-iastate.edu
} } www.baumlab.org
}
} --
} _____________________________________________________
} Tyrone L. Daulton
} Director: Marine Geosciences - Electron Microscopy Center
} Physicist and Senior Electron Microscopist
}
} Naval Research Laboratory
} Marine Geosciences Division (Code 7400)
} Stennis Space Center, MS 39529-5004
}
} Voice (228)-688-4877
} Fax (228)-688-4093
} email tdaulton-at-nrlssc.navy.mil
} tld-at-howdy.wustl.edu
}



From daemon Tue Nov 6 13:09:54 2001



From: Angela Klaus :      avklaus-at-amnh.org
Date: Tue, 6 Nov 2001 14:00:30 -0500 (EST)
Subject: Info needed: JEOL 100 CX TEMSCAN

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Hi all...

Does anyone know what type of microscope the JEOL 100 CX TEMSCAN is/was?
I've come across a reference to it in a 1982 paper by Eddy and Koehler and
need to describe the SEM imaging technology for something I'm writing.

Thanks in advance for any help.

Best,

Angela

Angela V. Klaus

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977



From daemon Tue Nov 6 13:57:38 2001



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu
Date: Tue, 06 Nov 2001 15:47:38 -0500
Subject: beer fund

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Let's take Nestor OUT for beer at the next microscopy meeting. Who says
scientists aren't any fun?

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Tue Nov 6 13:58:45 2001



From: Louis Somlai :      lssomlai-at-www.psrc.usm.edu
Date: Tue, 6 Nov 2001 14:32:01 -0600 (EST)
Subject: zooming website needed

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Dear All,

I think someone had once posted a URL to a website with an HTML
presentation that moved the observer from the sub-atomic viewing level to
one outside of our solar system using metric increments. Could someone
please send me that URL again?

Thanks,

Louis
Louis.Somlai-at-usm.edu




From daemon Tue Nov 6 15:16:33 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Tue, 6 Nov 2001 16:06:14 -0500
Subject: RE: Beer fund

Contents Retrieved from Microscopy Listserver Archives
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I still say it would be easier to have a sampling of beer, and, Oh, alright,
tell Nestor how good it (OR THEY!!!!) WAS!!!!

Fred Monson
I'm heading out now to start!!! See all of you who are down-time from me,
later!!!
} ----------
} From: Arthur Day
} Sent: Tuesday, November 6, 2001 2:43 AM
} To: Sally Stowe
} Cc: microscopy-at-sparc5.microscopy.com; Edward_Principe-at-amat.com
} Subject: Re: Beer fund
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Ok Sally I admit that last message was a bit ambiguous. I'll blame
} the Melbourne Cup BBQ for that (beer). So how do I weasel out of it?
}
} Nestor wrote: "Now if I only had a beer for each of those emails,
} I'd be set for life."
}
} If nature abhors a vacuum/empty stomach, and if there is no such
} thing as one beer, then each of us may have to contribute up to
} 100,000,000 beers each -- one per message. That's up to 100,000,000
} times 2562. So, therefore, it would be easier to send Nestor to each
} exotic location to sample the beer in person?
}
} Bit of an extrapolation I suppose.
}
} If anybody needs some tips for their next grant proposal.... ;-(
}
}
} } Maybe..
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } It would be easier to send Nestor to where the beer is. Less postage
} } that way...
} }
} } }
} } } I say we all mail Nester one beer, something regional or unique. Just
} } } picture the scene :-)
} } }
} } }
} } } -Ed
}
}
}


From daemon Tue Nov 6 17:09:58 2001



From: Paul Webster :      pwebster-at-hei.org
Date: Tue, 06 Nov 2001 15:06:42 -0800
Subject: SEM: how many samples

Contents Retrieved from Microscopy Listserver Archives
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Comments to comments:
}
} I'm usually wary when someone is fixated on a particular
} "magic" number, 30 or 300 or whatever. Often you are
} dealing with a person who doesn't understand statistics
} nearly as well as they think they do. Since you haven't
} been able to convince her of your position, maybe you can
} ask her to justify hers? Make her really explain (and
} hopefully understand) where that 30-40 target comes from.
} I don't necessarily *recommend* this approach, since it
} may lead to more conflict than collaboration!...
}
} Matt



} } } } My addition; If this person is so hung up on doing 30-40
} } } } samples, peerhaps you can give him'her instructions n sample prep
} } } } and then tell him'her that s/he should feel free to run up all
} } } } the samples on his/her time and that you would then gladly 'scope
} } } } them. I have found that when people learn by personal
} } } } experience how long it takes to do even a "standard' TEM
} } } } embedding they suddenly readjust their "n" requirement to a more
} } } } realistic goal.
Lee

Reply:
Unfortunately (or fortunately), the actual sample number is not arrived at
in an arbitrary manner, it is dictated by the nature of the specimen under
study.

Nobody makes up these numbers, but if you look at the article by John
Lucocq, Trends in Cell Biology 1993 Unbiased 3-D quantitation of
ultrastructure in cell biology 3:354-358, he suggests using 3-5
experiments/animals/cultures, only 1 or more blocks/dishes, one or more
sections but 10-40 micrographs and between 100-200 probe counts. I am
pretty sure this is a common sampling scheme for stereologists.

Paraphrasing slightly form this manuscript, Sampling is "..an inherent part
of any quantitative electron micrscopy study since only a portion of all the
possible blocks, sections and micrographs are analyzed. It is essential
therefore that at each level in a sampling scheme items are selected in a
way that gives all parts of the specimen the same chance of being included."

The actual amount of sampling that is performed will be directly related to
the variance occurring within the population. Samples with low variance
will require a lower sample number to give you an answer with an error of 5%
or less. High variance between individual samples will require more
sampling to be performed.

These are all experimental design considerations that have to be applied
much before all the clever statistical analysis is performed. Working out
probabilities on poorly sampled data is not very useful.

It can be said that proper sampling techniques should also be applied to
morphological data too. Where would that put all those text-book images?

Paul Webster.



From daemon Tue Nov 6 20:01:22 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 06 Nov 2001 17:46:57 -0800
Subject: Re: bacillus anthracis

Contents Retrieved from Microscopy Listserver Archives
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AIDS is a name of the illness caused by VIRUS, I believe. Virus itself has
a name: HIV, Human Immuno-deficit Virus.
Sergey

At 10:36 AM 11/6/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Tue Nov 6 20:17:44 2001



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 6 Nov 2001 21:12:19 -0500
Subject: Virus and Bacterium

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Yes, Ebola is a virus, AIDS is caused by a virus and Anthrax is
caused by a bacterium. How come I, as a lowly physicist, know this
but others do not, although they take great delight in trumpeting
their ignorance for all of us to see!

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} AIDS is a name of the illness caused by VIRUS, I believe. Virus
} itself has a name: HIV, Human Immuno-deficit Virus.
} Sergey
}
} At 10:36 AM 11/6/01, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Note new cell phone number!

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"


From daemon Tue Nov 6 20:35:25 2001



From: pcy :      pcy-at-usc.edu
Date: Tue, 6 Nov 2001 18:27:41 -0800 (PST)
Subject: Alkaline Phosphatase staining problems

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Dear Listpeoples

I'm a bit of a novice with antibody staining, but so are my colleagues and
so they've all got conflicting and inconsistent results from trying the
Alkaline Phosphatase staining.

We're trying to stain sea urchin larvae whole-mounts with the 2
antibody-alkaline phosphatase system(larvae are formalin fixed and
rehydrated from 70% EtOH cold storage). Our AkPh substrate is a few months
old and has a small amount of blackish precipitate at the bottom--is this
OK? My colleagues have had different results with using the substrate
undiluted on the animals vs. diluted in some small amount of PBS. I notice
that with the latter, there is a slight whitish precipitate that
occurs--is this due to the pH differential between the AkPh substrate(ph
10 or more) and PBS(7.4)? What are the ideal staining conditions for this
to work?
Please note--I'm getting problems with the preliminary staining where I
know I'm supposed to get endogenous gut AkPh staining with just substrate
and nothing else, but I'm getting almost imperceptible staining.

All suggestions and admonishments will be taken into consideration. Thanks
in advance.

Pauline Yu
pcy-at-usc.edu
Manahan Research Laboratory
http://www.usc.edu/manahanlab
University of Southern California



From daemon Tue Nov 6 22:39:35 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 6 Nov 2001 20:14:01 -0800
Subject: Teacher needs help

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There's a high school teacher in Hull, Massachusetts with 20 or so
microscopes that need cleaning and (hopefully minor) repair. She's willing
to work on them herself, but doesn't know how to begin. Is there a lister
who can visit and help her get started? If so please contact me.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Tue Nov 6 22:59:23 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Tue, 06 Nov 2001 23:53:48 -0500
Subject: Re: Image plates and CCD in very high voltage TEM

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on 11/4/01 4:39 PM, Victor Sidorenko at antron-at-space.ru wrote:

}
} Does anybody have the information about the using possibility of
} image recording systems (like PIXsysTEM from JEOL or the
} same from Fuji) and CCD cameras (for example from Gatan or
} another companies) in 1,000 kV TEM?
}
Dear Victor,
A few years ago (when the 1250 kV scope had been installed at Stutgart,
but I don't remember the exact year) there was a poster at MSA which gave
the quantum efficiency for image plates as a function of voltage up to 1250
kV. Since I was interested in quantitating ED data at 1200 kV, and since I
had heard great things about IPs--single-electron sensitivity, linearity for
a range which would include all the expected intensities in an ED pattern,
etc.--I remember the graph well. The QE for somewhat more than 100 kV was
100% (giving the required sensitivity), but it dropped off at intermediate
voltages--I don't remember whether it was still 100% at 200 kV, but
definitely lower at 400 kV--and was only ~25% at 1000+ kV. I don't know
what improvements may have been made; a thicker active layer will give more
sensitivity, but lower spatial resolution (there is no free lunch). The
manufacturers of the plates would be able to tell you whether they can sell
you what you would need. Good luck.
Yours,
Bill Tivol



From daemon Wed Nov 7 00:14:49 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Wed, 07 Nov 2001 16:38:56 +1030
Subject: Australian 2002 EM Conference

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ACEM 17
The 17th Australian Conference on Electron Microscopy
Adelaide Convention Centre, Adelaide, South Australia
February 4 - 8, 2002
Hosted by Australian Society for Electron Microscopy (Inc.)

This meeting will provide an opportunity to explore the many
applications of Electron, Scanned Probe and Confocal Microscopies, to
keep abreast of the latest developments and to hear and interact with
many of the leaders in the field. Sessions on Cryotechniques,
Virology/Microorganisms, Cell Tomography, Nanotechnology, Emerging EM
Technologies, Materials and Microstructure, Superresolution and an
Industry Forum are being planned, among many others.

Invited and Keynote Speakers (to date)
Martin Bootman; Confocal (biological), David Cockayne; Nanostructure,
Patrick Echlin; Analytical Microscopy of Biomaterials,
Mark Fricker; Confocal (biological), David Jefferson; Emerging
Techniques,
Jan Leunissen; Immunolabelling, Ohad Medalia; Cellular Tomography,
Greg Meeker; Microbeam Analysis, Sara Miller; Virology,
Daniel Mueller; SPM - (biological), David Williams; Microanalysis,
Nestor Zaluzec; Analytical Microscopy/Telepresence
Jamie Chapman; Reproductive Biology, Jacques Dubochet; Cryotechniques,
Alex Hyatt; Virology/Cryotechniques, Sergei Magonov; SPM,
Mary, Mah-Lee Ng; Virology/Confocal


A series of pre-conference workshops will be held to allow participants
to gain hands-on experience in the latest techniques and applications.
Many of the invited and keynote speakers will participate as presenters,
providing a wonderful opportunity for interaction with these noted
scientists. Be sure to book soon, as places in some of the workshops are
limited.

Workshops
Cryo Scanning Electron Microscopy
Environmental Scanning Electron Microscopy, Techniques and Applications
Image Analysis
Leica-Aurion Workshop on Cryotechniques and Immunolabelling
Light Element Analysis: Problems and Solutions
Quantitative Confocal Microscopy
Scanning Probe Microscopy
Trace and Major Quantitative X-ray Mapping (a Hands on Approach)
Virology Using Electron & Light Microscopy

During the week, delegates will have the chance to mingle and relax
after hours in several of Adelaide's noted heritage locations. A range
of accommodation choices, from five-star to budget, ensures choices
suitable for all.

The Trade Exhibition will be a comprehensive and exciting display of the
latest equipment and accessories for microscopy, imaging and analysis.
We are most grateful to our sponsors and exhibitors for their very
generous support of ACEM17.

As is traditional at all ACEMs, Poster and Micrograph Competitions will
be run.
Categories for the Poster Competition will be Best Biological Poster,
Best Physical Poster and Best Student Poster.
Categories for the Micrograph Competition are SEM, TEM, SPM and
Confocal. Entries will be judged on aesthetic, photographic and
scientific merit. Entry details are available on the website.

With the deadline for abstract submission fast approaching, (November
30), make plans now to join us in February! (Early bird registration
deadline - November 16). Abstracts should be submitted electronically,
and the registration form can be downloaded from the website - visit
often for regular updates.

We look forward to seeing you at ACEM17.

Full details can be obtained at
http://www.adelaide.edu.au/CEMMSA/acem17

Enquiries to:
ACEM17-at-all-occasions.com.au


From daemon Wed Nov 7 02:29:14 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 7 Nov 2001 08:31:01 +0000 (GMT Standard Time)
Subject: Re: Info needed: JEOL 100 CX TEMSCAN

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Hi Angela,

} From memory.

The JEOL 100CX TEMSCAN is a 100kV transmission electron
microscope (TEM) with the attachment for scanning imaging
device (ASID) that provides scanning electron microscope
(SEM) facilities (the SCAN part of TEMSCAN). It could image
with secondary electrons (SE) or backscattered electrons
(BSE) in a standard SEM mode or use brightfield or
darkfield detectors for scanning transmitted electron
microscopy (STEM). With an energy dispersive x-ray (EDX)
specrometer fitted it could also provide chemical
analysis, x-ray maps and line scans.

Undoubtably there are still some out there being used and
their users could give you more information but your local
JEOL office could also give you any information you want
(ask for an engineer over 40). If you don't know the local
JEOL office look on the web, although they maybe rushing
out to you now in case you want to buy something!!!

Regards,
Ron

On Tue, 6 Nov 2001 14:00:30 -0500 (EST) Angela Klaus
{avklaus-at-amnh.org} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all...
}
} Does anyone know what type of microscope the JEOL 100 CX TEMSCAN is/was?
} I've come across a reference to it in a 1982 paper by Eddy and Koehler and
} need to describe the SEM imaging technology for something I'm writing.
}
} Thanks in advance for any help.
}
} Best,
}
} Angela
}
} Angela V. Klaus
}
} Director, Core Imaging Facility
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Nov 7 03:40:31 2001



From: Steve Chapman :      protrain-at-emcourses.com
Date: Wed, 7 Nov 2001 09:34:25 -0000
Subject: Re: Info needed: JEOL 100 CX TEMSCAN

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Hi

The 100CX TEMSCAN is a JEOL 100kV TEM fitted with a scanning attachment
which allows imaging by way of "in lens" surface studies (SEM) in addition
to transmitted electron scanning (STEM). The microscope was a second
generation 100C hence the X.

The instrument was designed by Yanaka and Shirota the scanning system
interface was made by Koike (the man who invented the in lens SEM system).
Koike went on to design the ISI DS130 SEM and Yanaka and Shirota the ISI
002B TEM.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com




From daemon Wed Nov 7 03:40:52 2001



From: Steve Chapman :      protrain-at-emcourses.com
Date: Wed, 7 Nov 2001 09:34:25 -0000
Subject: Re: Info needed: JEOL 100 CX TEMSCAN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

The 100CX TEMSCAN is a JEOL 100kV TEM fitted with a scanning attachment
which allows imaging by way of "in lens" surface studies (SEM) in addition
to transmitted electron scanning (STEM). The microscope was a second
generation 100C hence the X.

The instrument was designed by Yanaka and Shirota the scanning system
interface was made by Koike (the man who invented the in lens SEM system).
Koike went on to design the ISI DS130 SEM and Yanaka and Shirota the ISI
002B TEM.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com




From daemon Wed Nov 7 07:19:50 2001



From: Platek, Frank :      FPLATEK-at-ora.fda.gov
Date: Wed, 7 Nov 2001 08:11:32 -0500
Subject: TEM: JEOL 100 CX - info and offer

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Hello to the Group,

Angela Klaus wrote: Does anyone know what type of microscope the JEOL 100 CX
TEMSCAN is/was?


If we're talking about the same instrument, the JEOL 100 CX that I spent
years on was a TEM (100 kV with a booster switch to 120 kV accelerating
voltage). Mine had the SAED (selected area electron diffraction) accessory
and Scanning EM functions.

Coincidentally, I know where just such an instrument is available for the
cost of tear down and shipping to any federal institution OR an institution
with an active NSF grant using electron microscopy. It includes a
full-sized, pneumatic anti-vibration table, chiller, compressor, film
carriers, etc.

Contact me off-line if interested and I'll see if it's still available.

S. Frank Platek
Forensic Chemistry Center
U.S. Food and Drug Administration
6751 Steger Drive
Cincinnati, OH 45237-3097
(513) 679-2700 X254
(513) 679-2761 FAX
fplatek-at-ora.fda.gov




From daemon Wed Nov 7 08:07:09 2001



From: JHoffpa464-at-aol.com
Date: Wed, 07 Nov 2001 09:01:22 EST
Subject: philly area only

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anyone in the philly area interested in getting together for a happy hour in town?


From daemon Wed Nov 7 09:51:59 2001



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Wed, 07 Nov 2001 10:46:17 -0500
Subject: question about energy resolution for a LaB6 TEM

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Hi,

We are interested in doing EELS work with a LaB6 filament TEM. Can anyone
tell us how best is the energy resolution with this type of filament?

Thanks

Best Regards
Yan Xin
=======================================
Yan Xin (Ph.D)
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Wed Nov 7 09:58:34 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 7 Nov 2001 07:46:15 -0800
Subject: Re: zooming website needed

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

That would be "Powers of 10", by Eames. I know it as a video, book, and
flip book (see the MICRO bibliography, URL below), but not as a website.
Will you please post the URL on the list when you get it?


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Nov 7 11:17:52 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 7 Nov 2001 12:08:47 -0500
Subject: RE: Virus and Bacterium and Beer

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Absolutely correct John! And, since you know, I'll ask.

Which physicist is most responsible for "nucular" physics [did I spell it
right???], and which Computer Scientist for "data is"?

I know the biologists are responsible for "dIsection"!

Blessings upon the lexicographers who say, "Cut it any way you want! DIsect
it with a cleaver or DISsect it with fine forceps. Your choice, whether you
know the difference or not!"

As Grandfather used to say when Grandmother was near, "Oh! For the love of
words!!!"

Fred Monson, who hath some affection for the silly!

P.S. By the way, Nestor, I stopped on the way home last evening, tried a
few different brands to be found in Eastern Pennsylvania, just Northwest of
Philadelphia, and most were worth the try. Every one went down as a toast
to your good works, and that was good too! So, you are taken care of here,
and I hope you appreciate the effort on your behalf. FCM

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/


} ----------
} From: John F. Mansfield
} Sent: Tuesday, November 6, 2001 9:12 PM
} To: microscopy-at-microscopy.com
} Subject: Virus and Bacterium
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Yes, Ebola is a virus, AIDS is caused by a virus and Anthrax is
} caused by a bacterium. How come I, as a lowly physicist, know this
} but others do not, although they take great delight in trumpeting
} their ignorance for all of us to see!
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } AIDS is a name of the illness caused by VIRUS, I believe. Virus
} } itself has a name: HIV, Human Immuno-deficit Virus.
} } Sergey
} }
} } At 10:36 AM 11/6/01, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } AIDS isn't a virus either. More semantics.
} } }
} } }
} } } } Kristen Lennon wrote:
} } } }
} } } } }
} ------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} -----------------------------------------------------------------------.
} } } } }
} } } } } Semantics because this mistake has been making me crazy - ANTHRAX IS
} A
} } } } } BACTERIUM! This is a particularly important point because the word
} "virus"
} } } } } scares people silly. When I think of virus, I think of AIDS and
} Ebolla
} } } } } (sorry it that's misspelled). When I think of bacterium, I think
} } } antibiotic.
} } } } } No offense intended,
} } } } } Kristen
} } } } }
} } } } } Kristen A. Lennon, Ph.D.
} } } } } Department of Plant Pathology
} } } } } 351 Bessey Hall
} } } } } Iowa State University
} } } } } Ames, IA 50011
} } } } }
} } } } } 515-294-8854
} } } } } kalen-at-iastate.edu
} } } } } www.baumlab.org
} } } }
} } } } --
} } } } _____________________________________________________
} } } } Tyrone L. Daulton
} } } } Director: Marine Geosciences - Electron Microscopy Center
} } } } Physicist and Senior Electron Microscopist
} } } }
} } } } Naval Research Laboratory
} } } } Marine Geosciences Division (Code 7400)
} } } } Stennis Space Center, MS 39529-5004
} } } }
} } } } Voice (228)-688-4877
} } } } Fax (228)-688-4093
} } } } email tdaulton-at-nrlssc.navy.mil
} } } } tld-at-howdy.wustl.edu
} } } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
}
}
} --
} Note new cell phone number!
}
} Dr. John Mansfield CPhys MInstP
} North Campus Electron Microbeam Analysis Laboratory
} 417 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (734) 936-3352 FAX (734) 763-2282
} Cellular Phone: (734) 834-3913
} (Leaving a phone message at 936-3352 is preferable to 834-3913)
} Email: jfmjfm-at-engin.umich.edu
} URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
} Location: Lat. 42° 16' 48" Long. 83° 43' 48"
}
}


From daemon Wed Nov 7 11:35:44 2001



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Wed, 07 Nov 2001 12:30:36 -0500
Subject: GaN/InN etch

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Fellow microscopists,

Does anyone know a recipe for chemically etching GaN and/or InN? The
material is already thinned and ready for TEM, we just want a final surface
etching to enhance dislocation imaging.

Thanks,

Mick

-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Wed Nov 7 12:23:57 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 7 Nov 2001 13:12:42 -0500
Subject: RE: Info needed: JEOL 100 CX TEMSCAN

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Hi Angela,

Try this: http://www.deltacollege.org/dept/electmicro/history.html

Fred Monson


Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Angela Klaus
} Sent: Tuesday, November 6, 2001 2:00 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Info needed: JEOL 100 CX TEMSCAN
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all...
}
} Does anyone know what type of microscope the JEOL 100 CX TEMSCAN is/was?
} I've come across a reference to it in a 1982 paper by Eddy and Koehler and
} need to describe the SEM imaging technology for something I'm writing.
}
} Thanks in advance for any help.
}
} Best,
}
} Angela
}
} Angela V. Klaus
}
} Director, Core Imaging Facility
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} Email: avklaus-at-amnh.org
} Tel: 212-769-5977
}
}
}


From daemon Wed Nov 7 12:42:03 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Wed, 7 Nov 2001 13:30:23 -0500
Subject: LM & TEM Processing of tendon samples

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} -----Original Message-----
} From: Sy Griffey
} Sent: Wednesday, November 07, 2001 11:48 AM
} To: Jacqueline D. Garfield
} Subject: Processing of tendon samples
}
}
}
} If anyone has some experience processing human tendon samples for EM
} and/or histology we would appreciate your help.
}
} Problem: We have begun processing human tendon samples for both EM and
} standard histology but are having trouble getting good sections and tissue
} quality. The samples for histology can be as thick as 5-8 mm.
}
} For the EM samples we have only done a freeze-substitution protocol
} to date but the tissue was extremely hard before embedding and even thick
} sectioning was a challenge. We anticipate similar problems when doing
} standard EM processing based on experience with histology processing thus
} far........The tissue in the histology sections appears cracked,
} exhibiting separation within the fibrous bundles of the tendon when viewed
} in X-sxn. The samples were actually somewhat flexible just prior to
} embedding. All processing steps (graded alcohol, clearing solution and
} paraffin) have been done at ambient temperature and pressure. The
} incubation times, in general, have been in the 45-60 min. range. We are
} thinking about increasing the incubation times and perhaps doing some
} vacuum infiltration but would welcome any suggestions.
}
} Thank you!
}
} Jackie Garfield and Sy Griffey
}
} Lifecell Corporation
} One Millennium Way
} Branchburg, NJ 08876 USA
} (908) 947-1182 (Jackie)
} (908) 947-1085 (Fax)
} jgarfield-at-lifecell.com


From daemon Wed Nov 7 12:44:13 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 8 Nov 2001 07:38:09 GMT+1200
Subject: Bell Jar solved

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Thank you for the many replies regarding the problem of
firmly-attached carbon.

It appears that in removing the metal coating left on the glass
surface in the bell jar's previous life in a physics lab, the aqua
regia that I ended up using activated the glass surface, perhaps by
producing a micro-roughness. So the first few uses produced a
firmly-attached carbon coating.

I was able to remove the carbon with a mildly-abrasive ("without
harsh scratching") household cleaner, plus lots of rubbing.

I then wiped the surface with a rag wetted with kerosine, then with a
clean dry rag. This left sufficient kerosine to prevent a good vacuum
being obtained, so I sprayed it with a household
general-purpose multi-solvent cleaner ("Ajax Spray 'n' Wipe), and
wiped that off with a clean dry rag.

This has left the surface sufficiently deactivated so that I can
remove new carbon simply by spraying with Spray 'n' Wipe and wiping.

cheers

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Wed Nov 7 15:03:24 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 7 Nov 2001 15:53:37 -0500
Subject: Re: How to sample?

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Hypothetical!

A graduate student in microbiology has just finished her first course in
electron microscopy, and she informs her advisor that she wants to study
mitochondrial structure and function in several related strains of yeast,
for one of which she fortuitously has electron micrographs of mitochondrial
profiles that are, she has been told, quite publishable.

Her advisor suggests that she start by comparing 3-4 strains with respect to
number of mitochondria per cell and total mitochondrial cristal area under
identical conditions of culture.

The student happily responds that she already has micrographs for regularly
sampled sections from serial sections she learned to make of the strain she
used in her EM course. She returns the next week with estimates of cristal
area and numbers of mitochondria per cell based on morphometric analysis of
those regularly sampled sections.

If the cell is 12-25um in length (haploid and diploid) and always between
3-5um in diameter, and if the mitochondrial profiles are in the range of
1-2um (circular and ovoid, and sometimes or often branched), what should
the sampling criteria be for cells that are serially sectioned with sections
approximately 90nm in thickness?

How could one derive the same, but apparently much more statistically
satisfactory, data from the 40+ cell profiles per section of cells randomly
oriented and not necessarily (or importantly) sectioned or analyzed
completely.

All who take on this task should know there is an extremely important, and
possibly significant, hint that can be observed at:
http://www.msg.ucsf.edu/sedat/marsh/mito.html. I sometimes think it is well
to know of such hints (or at least to be aware of their possibility) before
attempting a task. Assumptions are almost always incorrect unless
rigorously defined and applied, and very often difficult to prove as such or
to isolate in generating an experimental design without flaws.

Knowing that reality often gets in the way of statistics, and vice versa, I
am

Yours most respectfully, because I wrestle with this problem every once in a
while and I haven't yet determined a satisfactory answer.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Wed Nov 7 15:46:18 2001



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Wed, 07 Nov 2001 16:46:54 -0500
Subject: Powers of 10 Website

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I just looked in Yahoo and found the website. It's www.powersof10.com.

Edward Haller
University of South Florida
Pathology Department
Tampa, Florida


Caroline Schooley wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear All,
} }
} } I think someone had once posted a URL to a website with an HTML
} } presentation that moved the observer from the sub-atomic viewing level to
} } one outside of our solar system using metric increments. Could someone
} } please send me that URL again?
} }
} } Thanks,
} }
} } Louis -
}
} That would be "Powers of 10", by Eames. I know it as a video, book, and
} flip book (see the MICRO bibliography, URL below), but not as a website.
} Will you please post the URL on the list when you get it?
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From daemon Wed Nov 7 15:54:21 2001



From: Boche, Jo Ellen :      bochej-at-boystown.org
Date: Wed, 7 Nov 2001 16:32:57 -0600
Subject: Cutting thin sections from thick.

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Can anyone in the DFW area perform these types of analysis? Respond to me
with details and contact information.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu

-----Original Message-----
} From: Rajan Vempati [mailto:rvempati-at-mail.smu.edu]
Sent: Wednesday, November 07, 2001 10:16 PM
To: rbeavers-at-mail.smu.edu


I am looking for a simple, reliable method for obtaining thin sections
for EM from thick (1-2 microns) epoxy resin sections that have been cut,
mounted on slides, and stained for preliminary examination with the LM
.


From daemon Wed Nov 7 16:49:42 2001



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Wed, 7 Nov 2001 17:44:00 -0500
Subject: Zooming website needed

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Hi All,
After Caroline's message I did a web search for "powers of 10" and
found this web site which seems to fit what was requested.
http://www.wordwizz.com
Steve

Steve Buckingham
Dir. of Process Development
Excellatron Solid State LLC
1640 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 617 812 5920





From daemon Wed Nov 7 16:56:43 2001



From: Boche, Jo Ellen :      bochej-at-boystown.org
Date: Wed, 7 Nov 2001 16:51:01 -0600
Subject: TEM Request for method for cutting thin sections from thick.

Contents Retrieved from Microscopy Listserver Archives
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} -----Original Message-----
} From: Boche, Jo Ellen
} Sent: Wednesday, November 07, 2001 4:33 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: TEM Request for method for cutting thin sections from
} thick.
}
}
}
} I am looking for a simple, reliable method for obtaining thin sections
} for EM from thick (1-2 microns) epoxy resin sections that have been
} cut, mounted on slides, and stained for preliminary examination with
} the LM.
}
} bochej-at-boystown.org
}
} Jo Ellen Boche
} Neuroanatomy Laboratory
} Boys Town National Research Hospital
} Omaha, NE 68131
} (402) 498-6531
}
} .


From daemon Wed Nov 7 18:57:45 2001



From: Tamara Howard :      thoward-at-UNM.EDU
Date: Wed, 7 Nov 2001 17:50:44 -0700 (MST)
Subject: Re: TEM Request for method for cutting thin sections from thick.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can "just" re-embed the sections directly on the slide (invert the
slide-bound sections on BEEM capsules filled with resin or put the
capsule on the slide), repolymerize, and cut. I always manage to make a
serious mess with the resin, but it does work.

You may also be able to glue the thick section to a polymerized blank
block and then pop it off the slide with a heat/cold regimen - I haven't
tried this but it is supposed to work. I think it may be more useful/less
disastrous with a REALLY thick section than a normal thick section.

Good luck!

Tamara

On Wed, 7 Nov 2001, Boche, Jo Ellen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} } -----Original Message-----
} } From: Boche, Jo Ellen
} } Sent: Wednesday, November 07, 2001 4:33 PM
} } To: 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: TEM Request for method for cutting thin sections from
} } thick.
} }
} }
} }
} } I am looking for a simple, reliable method for obtaining thin sections
} } for EM from thick (1-2 microns) epoxy resin sections that have been
} } cut, mounted on slides, and stained for preliminary examination with
} } the LM.
} }
} } bochej-at-boystown.org
} }
} } Jo Ellen Boche
} } Neuroanatomy Laboratory
} } Boys Town National Research Hospital
} } Omaha, NE 68131
} } (402) 498-6531
} }
} } .
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|



From daemon Wed Nov 7 20:14:14 2001



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 07 Nov 2001 18:01:41 -0800
Subject: Re: How to sample?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good point Fred

For this reason it's more correctly to call it: "mitochondrion" (M),
instead mitochondria. In my point of view, on EM level only 3D
reconstruction from the serial sections may give you truthful info about
this structure.

By the way, mitochondria/mitochondrion is known as an extremely sensitive
organelle: to oxidative stress etc&etc. Its structure may be changed by
very slight variation of condition/procedure. Simple example: you are
looking how some particular mutation affected M structure. It's very
possible, that your mutation will affect the membrane permeability nor M at
all. On EM level this may cause the different conditions during sample
fixation/processing. Finally you will detect M changes, because the
processing conditions were different for control and mutant. Another
example: mutants are growing slowly at many instances, it's very difficult
to have the culture's "age" similar. People usually grow cells to the some
particular optical density (at 450 nm?). So the control will grow up to 1
OD, let say 5 h, but the mutant - 18+ h. Are the "age" of the cells the
same? I guess, not. The structure of the M in the cells of different
"age" may (and could) be different. So, we have to be careful with M as,
from another hand, all EM stuff we have deal with.
Sergey

At 12:53 PM 11/7/01, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From daemon Wed Nov 7 21:25:42 2001



From: george_greg-at-msn.com ()
Date: Wed, 7 Nov 2001 21:15:41 -0600
Subject: Ask-A-Microscopist: LM question about oil immersion lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (george_greg-at-msn.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
November 7, 2001 at 19:50:43
---------------------------------------------------------------------------

Email: george_greg-at-msn.com
Name: Greg George

Education: 9-12th Grade High School

Location: Pearl, Mississippi USA

Question: Dear Sir,
I recently received, as a gift, a used lab grade microscope without a
user manual. Three of the four (oil immersion) objectives are spring
retractable. Does this feature mean that I can touch the slide
surface without fear of damaging the lens?
Thank you for your kind reply,
Greg George


---------------------------------------------------------------------------


From daemon Wed Nov 7 21:43:14 2001



From: Powers, Christine :      Christine.Powers-at-umassmed.edu
Date: Wed, 7 Nov 2001 22:31:47 -0500
Subject: Re: zooming website needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I missed the original posting, but this may be the link you're looking for:

http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/index.h



Christine Powers
UMass Medical School
Biotech 4, 3rd floor
377 Plantation St.
Worcester, MA 01605


-----Original Message-----
} From: Caroline Schooley
To: Louis Somlai
Cc: Microscopy-at-sparc5.microscopy.com
Sent: 11/7/01 10:46 AM


} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

From daemon Wed Nov 7 22:06:37 2001



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Thu, 8 Nov 2001 09:33:04 +0530
Subject: Re: zooming website needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Powers of 10 is also available as a Java applet at
http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/
At this moment, the website is inaccessible to me so I cannot verify for sure.
Best Wishes.

-----Original Message-----
} From: Caroline Schooley [SMTP:schooley-at-mcn.org]
Sent: Thursday, November 08, 2001 9:25 AM
To: Louis Somlai
Cc: Microscopy-at-sparc5.microscopy.com


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

That would be "Powers of 10", by Eames. I know it as a video, book, and
flip book (see the MICRO bibliography, URL below), but not as a website.
Will you please post the URL on the list when you get it?


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html







From daemon Wed Nov 7 23:02:55 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Thu, 8 Nov 2001 14:58:24 +1000
Subject: RE: zooming website needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sounded interesting. So I did an "advanced search" for the phrase "power of
ten" using www.google.com The first three URLs were:

http://www.powersof10.com/
http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/
http://www.cacr.caltech.edu/~roy/dataquan/

The first site mentions Eames and would be the wanted site. The second site too
is very good and worth a long look. Its part of the molecular expressions site
at Florida State Uni.
The third site gives a power of ten perspective on computer data sizes.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, November 08, 2001 1:46 AM, Caroline Schooley
[SMTP:schooley-at-mcn.org] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear All,
} }
} } I think someone had once posted a URL to a website with an HTML
} } presentation that moved the observer from the sub-atomic viewing level to
} } one outside of our solar system using metric increments. Could someone
} } please send me that URL again?
} }
} } Thanks,
} }
} } Louis -
}
} That would be "Powers of 10", by Eames. I know it as a video, book, and
} flip book (see the MICRO bibliography, URL below), but not as a website.
} Will you please post the URL on the list when you get it?
}
}
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}



From daemon Thu Nov 8 00:58:05 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 08 Nov 2001 01:50:16 -0500
Subject: Re: question about energy resolution for a LaB6 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 11/7/01 10:46 AM, Yan Xin at xin-at-magnet.fsu.edu wrote:
} We are interested in doing EELS work with a LaB6 filament TEM. Can anyone
} tell us how best is the energy resolution with this type of filament?
}
Dear Yan Xin,
As I remember it, the energy spread in a LaB6 filament is about 1-2 eV.
The energy resolution obtainable in EELS is affected by factors other than
the filament, however, and I do not know the best that can be obtained with
your instrument.
Yours,
Bill Tivol



From daemon Thu Nov 8 01:48:52 2001



From: greenwood :      greenwoo-at-blue.weeg.uiowa.edu
Date: Thu, 8 Nov 2001 01:41:55 -0600
Subject: RE: Virus and Bacterium and Beer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are trying to section a tampon in to about five micrometers but any normal
sample prep involves EtOH or some other fluid that will be absorbed. i was
wondering if anyone knew a method to keep it in at its normal size but in a
form that can be cut very thin
thanks
john

} ===== Original Message From "Monson, Frederick C." {fmonson-at-wcupa.edu} =====
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Nov 8 03:15:34 2001



From: Mike Wombwell :      mike.wombwell-at-vgscientific.com
Date: Thu, 8 Nov 2001 09:00:39 -0000
Subject: Polaron Range Changes Hands... New Contact Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



With effect from 1st November 2001 the Polaron Range of EM preparation
instruments will change ownership from Thermo VG Scientific to Quorum
Technologies Ltd.

Quorum Technologies Ltd. is the company formed by Bob Kenhard, Mike
Wombwell, David Cheshire and the existing staff within Thermo VG Scientific
involved in the management, development, sales and manufacturing of the
Polaron Range.

We will continue to support the many Polaron instruments out in the field
(including EM preparation instruments branded Polaron Equipment Ltd,
Emscope, Bio-Rad Microscience, Fisons Instruments and VG). Additional
details are available on the new Quorum WWW site (
http://www.quorumtech.com).

The new contact address (effective Nov. 12) will be

Quorum Technologies
Unit 15A
Euro Business Park
New Road
Newhaven
East Sussex
U.K.
BN9 0DQ

Tel: ++ 44(0) 1273 510535
Fax: ++ 44(0) 1273 510536

Contacts:
Mike.wombwell - sales (mike.wombwell-at-vgscientific.com - after 12th November
- mike.wombwell-at-quorumtech.com)
David Cheshire - technical support (david.cheshire-at-vgscientific.com - after
12th November - dave.cheshire-at-quorumtech.com)
Bob Kenhard - sales and other enquiries (bob.kenhard-at-vgscientific.com -
after 12th November - bob.kenhard-at-quorumtech.com)
WWW: http://www.quorumtech.com
US Contact: Energy Beam Sciences (telephone: 413 786 9322 E-mail -
sales-at-ebsciences.com)


Mike Wombwell
Polaron Range Sales Director
Quorum Technologies


From daemon Thu Nov 8 06:02:04 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Thu, 08 Nov 2001 12:58:43 +0100
Subject: Re: zooming website needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Go to the Google site and search on powers of 10

http://www.google.com/
http://www.google.com/search?q=powers+of+10&hl=sv&lr=

Good luck!


At 14:32 2001-11-06 -0600, Louis Somlai wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Thu Nov 8 07:46:30 2001



From: marian miller :      millermn-at-email.uc.edu
Date: Thu, 08 Nov 2001 08:37:16 -0500
Subject: microfillaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can someone give me an excellent fixative for microfillaments in
microvilli, particularly in the gut?

Stacey Andringa
University of Cincinnati



From daemon Thu Nov 8 08:35:14 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Thu, 08 Nov 2001 08:32:27 -0800
Subject: Re: zooming website needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's another website with a cool powers of 10 display.


http://microscopy.fsu.edu/primer/java/scienceopticsu/powersof10/index.html

Fred Schamber
ASPEX LLC

Louis Somlai wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All,
}
} I think someone had once posted a URL to a website with an HTML
} presentation that moved the observer from the sub-atomic viewing level to
} one outside of our solar system using metric increments. Could someone
} please send me that URL again?
}
} Thanks,
}
} Louis
} Louis.Somlai-at-usm.edu


From daemon Thu Nov 8 11:25:27 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 8 Nov 2001 10:58:51 -0600
Subject: Histo certification in Kansas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listies,

Would anyone know how to go about getting official certification for histo
in the KS and MO region.

Thanks
Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Thu Nov 8 12:10:26 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 8 Nov 2001 12:03:45 -0600
Subject: ESEM of cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,

I am looking for publications of cell cultures observation in a ESEM. So
far not a great success. Any help is greatly appreciated.

Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy




From daemon Thu Nov 8 12:40:34 2001



From: Mike Wombwell :      mike.wombwell-at-vgscientific.com
Date: Thu, 8 Nov 2001 18:28:37 -0000
Subject: Polaron Range Changes Hands... New Contact Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



With effect from 1st November 2001 the Polaron Range of EM preparation
instruments will change ownership from Thermo VG Scientific to Quorum
Technologies Ltd.

Quorum Technologies Ltd. is the company formed by Bob Kenhard, Mike
Wombwell, David Cheshire and the existing staff within Thermo VG
Scientific involved in the management, development, sales and
manufacturing of the Polaron Range.

We will continue to support the many Polaron instruments out in the
field (including EM preparation instruments branded Polaron Equipment
Ltd, Emscope, Bio-Rad Microscience, Fisons Instruments and VG).
Additional details are available on the new Quorum WWW site (
http://www.quorumtech.com).

The new contact address (effective Nov. 12) will be

Quorum Technologies
Unit 15A
Euro Business Park
New Road
Newhaven
East Sussex
U.K.
BN9 0DQ

Tel: ++ 44(0) 1273 510535
Fax: ++ 44(0) 1273 510536

Contacts:
Mike.wombwell - sales (mike.wombwell-at-vgscientific.com - after 12th
November - mike.wombwell-at-quorumtech.com)
David Cheshire - technical support (david.cheshire-at-vgscientific.com -
after 12th November - dave.cheshire-at-quorumtech.com)
Bob Kenhard - sales and other enquiries (bob.kenhard-at-vgscientific.com -
after 12th November - bob.kenhard-at-quorumtech.com)
WWW: http://www.quorumtech.com
US Contact: Energy Beam Sciences (telephone: 413 786 9322 E-mail -
sales-at-ebsciences.com)


Mike Wombwell
Polaron Range Sales Director
Quorum Technologies


From daemon Thu Nov 8 14:53:42 2001



From: Paul Webster :      pwebster-at-hei.org
Date: Thu, 08 Nov 2001 12:48:41 -0800
Subject: Re: How to sample?

Contents Retrieved from Microscopy Listserver Archives
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Hi Fred,

At the risk of looking very stupid, I will take your challenge.

Am I correct in understanding that your question has two parts? 1. What is
the mean number of mitochondria per yeast cell in a population. 2. What is
the mean area of cristae membranes.

If this is so, then there are actually three challenges here because to get
an absolute number for the cristae surface area and the number of
mitochondria per cell we also need to have an estimate for a reference space
in actual numbers. A good reference space to estimate is cell volume.

Obtaining mean number of mitochondria per cell is actually very easy. I
would first throw away the images already obtained because they were
subjectively sampled.

We must then remove bias that may be a result of centrifugation - heavier
(more dense) cells will pellet to the bottom, with the possibility that a
gradient is formed in the pellet. To avoid this, embed the cells first in
fast-setting gelatin or agarose, being careful to make sure the cells have
been thoroughly mixed. We could of course do it properly and apply the
"orintator" to make sure our sampling is isotropic (Mattfeldt et al 1989
Acta Stereol. 8:671-676).

Fix the block and cut it into slabs, rods and then cubes. Using a
sequential random sampling protocol, take every "nth" block (decided using
random number generator) and embed them separately in resin (see Lucucq 1993
Trends Cell Biol 3:345-358 for details).

Section for counting. A good way to sample the cells to count particles
(here is where the catch comes in) is to only count the tops of the
particles. With the yeast mitochondria, the 2-D profile does not always
represent an individual particle. A profile may represent one single
mitochondrion, or may represent a multiply branched, single mitochondrion,
depending on the level of branching that is occurring. By counting the
tops, connected profiles are eliminated from the sampled population. (for
details on a possible method for estimating the amount of branching that is
present, look for "Star Volume" in Gundersen et al 1986 J. Microsc.
143:3-45)

How to sample? Use the "Disector" (in this instance, the word means "2
sectors"). The reference is D.C. Stereo (a pseudonym for HJG Gundersen)
1984. J. Microsc. 134:127-136.

To apply, cut two sequential sections and take similar fields of view from
both. Use one field to examine for mitochondrial profiles and the other to
check if the profile is also present there ( the look-up frame). If a
profile is present in one field but not present in the other, it is counted
as a "top". (It is a variation of the 2-D counting rule, the associated
tangent rule).

Knowing the area of the field of view and the thickness separating the two
sequential sections, we can work out the volume being sampled. This will
give us an estimate of the number of mitochondria per unit volume. This is
not the number of mitochondria per cell. We still need to estimate the cell
volume.

To estimate surface area, we can use simple cross lattice overlays and count
the number of times a membrane profile crosses a test line. From this we
can estimate length of membrane, and by knowing section thickness, can
obtain an estimate of membrane area. (see H.J.G. Gundersen et al 1986 J.
Microsc. 143:3-45. 1988 APMIS 96:379-394 & 857-881, T.M. Mayhew 1991 Exp.
Physiol. 76:639-663, J. Lucocq for details on how to do this) We still need
to estimate the cell volume.

How am I doing so far, Fred?

Now, a question for you. I can provide estimates of relative values for
number and surface area. However, I do need a reference space to obtain
absolute values. How would I obtain a reliable estimation of the mean cell
volume of the yeast population? Presumably they are a rapidly dividing
population.

Regards

Paul Webster.






on 11/7/01 12:53 PM, Monson, Frederick C. at fmonson-at-wcupa.edu wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hypothetical!
}
} A graduate student in microbiology has just finished her first course in
} electron microscopy, and she informs her advisor that she wants to study
} mitochondrial structure and function in several related strains of yeast,
} for one of which she fortuitously has electron micrographs of mitochondrial
} profiles that are, she has been told, quite publishable.
}
} Her advisor suggests that she start by comparing 3-4 strains with respect to
} number of mitochondria per cell and total mitochondrial cristal area under
} identical conditions of culture.
}
} The student happily responds that she already has micrographs for regularly
} sampled sections from serial sections she learned to make of the strain she
} used in her EM course. She returns the next week with estimates of cristal
} area and numbers of mitochondria per cell based on morphometric analysis of
} those regularly sampled sections.
}
} If the cell is 12-25um in length (haploid and diploid) and always between
} 3-5um in diameter, and if the mitochondrial profiles are in the range of
} 1-2um (circular and ovoid, and sometimes or often branched), what should
} the sampling criteria be for cells that are serially sectioned with sections
} approximately 90nm in thickness?
}
} How could one derive the same, but apparently much more statistically
} satisfactory, data from the 40+ cell profiles per section of cells randomly
} oriented and not necessarily (or importantly) sectioned or analyzed
} completely.
}
} All who take on this task should know there is an extremely important, and
} possibly significant, hint that can be observed at:
} http://www.msg.ucsf.edu/sedat/marsh/mito.html. I sometimes think it is well
} to know of such hints (or at least to be aware of their possibility) before
} attempting a task. Assumptions are almost always incorrect unless
} rigorously defined and applied, and very often difficult to prove as such or
} to isolate in generating an experimental design without flaws.
}
} Knowing that reality often gets in the way of statistics, and vice versa, I
} am
}
} Yours most respectfully, because I wrestle with this problem every once in a
} while and I haven't yet determined a satisfactory answer.
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} CASI Home Page: http://darwin.wcupa.edu/casi/
} South Church Street
} West Chester, PA, 19383
} Office: SS024
} Phone: 610-738-0437
} FAX: 610-436-3036
} eMail: fmonson-at-wcupa.edu
} Please call before visiting
}
}



From daemon Thu Nov 8 15:05:24 2001



From: thurston e herricks :      thurst0n-at-u.washington.edu
Date: Thu, 8 Nov 2001 12:59:44 -0800 (PST)
Subject: Philips 400T IGP powersupply

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am looking for a transformer to go into the Ion getter pump powersupply
for a Philips 400T. The power supply transformer in this microscope is
fried and needs to be replaced. I'm not sure who to contact since the
microscope division of Philips is now owned by FEI. Any information would be
appreciated.

Take care

Thurston Herricks



From daemon Thu Nov 8 17:54:35 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 8 Nov 2001 17:46:13 -0600
Subject: MRC-1000 video woes

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We have a Biorad MRC-1000 (1994) with the original video card, and do not have a
service contract. The video signal goes into an Indec Systems box with an output
RGB + horiz. + vert. via BNC connectors to the monitor.
Lately we have been having sporadic severe problems where both the frame and
image do not appear on the monitor. Instead a series of dots and/or lines moving
across the monitor screen appear. This problem may persist for days and then
disappear and the normal frame and image reappear. Sometimes the problem appears
momentarily and then corrects. Jiggling the cables during the malfunction
changes the rate of dots and lines, but does not bring the frame or image back.
Most experts have said this is a monitor problem, but some suggest the video
card. The monitor itself is specialized in that ordinary BNC monitors will not
work satisfactorily (we have tried). A new monitor from Biorad is several
thousand dollars. We would hate to buy a new monitor only to discover it really
is the card.
Any suggestions as to what additional troubleshooting can be done, or other
insights, would be appreciated.
Bruce
Bruce Cutler, Ph.D.
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas




From daemon Thu Nov 8 19:28:34 2001



From: reimar_gaertner-at-wsib.on.ca ()
Date: Thu, 8 Nov 2001 19:18:53 -0600
Subject: Ask-A-Microscopist:LM Kohler Illumination Question

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (reimar_gaertner-at-wsib.on.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Thursday,
November 8, 2001 at 12:19:54
---------------------------------------------------------------------------

Email: reimar_gaertner-at-wsib.on.ca
Name: Reimar Gaertner

Organization: WSIB

Education: Graduate College

Location: Toronto, Ontario, Canada

Question: My 8-year-old daughter and I have developed an interest in
microscopy as a hobby.
We have a Meade 9400 student microscope with built-in light (no field
diaphram) and a fixed condenser (with a rotating aperture wheel).
I am able to "focus" the condenser by inserting blocks under the
slide (and lowering the stage to focus).
This has allowed me to get closer to the critical focusing position
(the surface of the light source now focuses about 1 cm above the
stage.
I need this for an effective dark-field effect at 100x magnification
(10x10). I have heard that in order to best see thick specimens
(especially at 400x), we need Kohler illumination.
Do you think this will really make a difference? If so, what
modifications would I need to make to achieve this?
Many thanks.
Reimar

---------------------------------------------------------------------------


From daemon Thu Nov 8 19:59:57 2001



From: Lundberg :      sawlundberg-at-home.com
Date: Thu, 08 Nov 2001 18:48:54 -0600
Subject: SEM Service Contract

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
I am looking for independent providers of service contracts for a
JSM-5310 SEM with Digital Image Grabber in Southern Arizona. I would
appreciate it if anyone who could provide this service, or anyone who
has experiences, good or bad, would email me off list.

Many thanks in advance,
Sarah Lundberg


From daemon Thu Nov 8 22:07:20 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 08 Nov 2001 22:52:36 -0500
Subject: Re: How to sample?

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on 11/8/01 3:48 PM, Paul Webster at pwebster-at-hei.org wrote:

}
} At the risk of looking very stupid, I will take your challenge.
}
} Am I correct in understanding that your question has two parts? 1. What is
} the mean number of mitochondria per yeast cell in a population. 2. What is
} the mean area of cristae membranes.
}
} If this is so, then there are actually three challenges here because to get
} an absolute number for the cristae surface area and the number of
} mitochondria per cell we also need to have an estimate for a reference space
} in actual numbers. A good reference space to estimate is cell volume.
[skip]
}
} Regards

} Paul Webster.

Dear Paul,
The risk of your looking stupid is very minimal. I'd use a
mito-specific dye and LM to count mitos/cell (and get the distribution of
cell sizes, info on whether [mitos] is greater for smaller/larger cells or
is constant, etc.). I also have a bias for using HVEM tomography on thick
sections to determine cristal-area/mito. Otherwise, yours seems like a very
good plan.
Yours,
Bill Tivol



From daemon Thu Nov 8 22:07:20 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 08 Nov 2001 22:57:03 -0500
Subject: Re: ESEM of cell cultures

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on 11/8/01 1:03 PM, Dusevich, Vladimir at DusevichV-at-umkc.edu wrote:

}
} I am looking for publications of cell cultures observation in a ESEM. So
} far not a great success. Any help is greatly appreciated.
}
Dear Vladimir,
I don't know about ESEM, but I did look at cultured PTK1 cells in an
environmental chamber in the HVEM (a TEM). I presented a few images at a
Biophysical Society meeting about 15 years ago, so I do not remember many
details. I'd be happy to discuss this with you off line if you want.
Yours,
Bill Tivol



From daemon Fri Nov 9 08:27:06 2001



From: Brad Jolliff :      blj-at-levee.wustl.edu
Date: Fri, 09 Nov 2001 08:16:04 -0600
Subject: uProbe - position opening, Washington University, St. Louis

Contents Retrieved from Microscopy Listserver Archives
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Dear MSA Listserver subscribers,

Following is an announcement of a position opening at Washington University
in St. Louis. I would appreciate your bringing this to the attention of
anyone who might be interested. The workhorses of our facilities are a
JEOL733 electron microprobe with Advanced Microbeam automation and image
analysis, and a Rigaku Geigerflex X-ray diffractometer. Please respond to
{blj-at-levee.wustl.edu} if you have any questions and not to the listserver.

Thanks!

Brad Jolliff
_________

Research Specialist/Electron Probe Microanalysis

Washington University in St. Louis is seeking a research scientist who will
operate and enhance the Electron Microprobe and X-ray Diffractometer
facilities and conduct independent and collaborative research in petrology
and geochemistry in the Department of Earth and Planetary Sciences. This is
a Research Specialist position and is not tenure-track. Duties will include
day-to-day management and operation of the EMP and XRD laboratories as well
as enhancement of systems and analytical capabilities. Ph.D. preferred. For
his or her research, the Specialist would have access to the wide array of
analytical facilities within the Department of Earth and Planetary Sciences
and in the McDonnell Center for the Space Sciences. These facilities
include microbeam techniques (SIMS, laser-ablation ICP-MS, Raman
spectroscopy), major- and trace-element chemical analysis (INAA, XRF,
ICP-MS), mass spectrometers for stable and radiogenic isotope analysis, and
a variety of other spectrometers including visible, IR, and Moessbauer.

To apply, submit a resume, statement of research interests, and names and
contact information of three professional references to:

Bradley L. Jolliff
Department of Earth and Planetary Sciences
Washington University
Campus Box 1169
One Brookings Drive
St. Louis, MO 63130

Applications should be submitted by Dec 21, 2001. Women and
underrepresented minorities are encouraged to apply. Washington University
is an equal opportunity/affirmative action employer. Employment eligibility
verification is required upon employment.
__________

department web site: {http://epsc.wustl.edu/}
__________

Bradley L. Jolliff, Ph.D
Research Associate Professor
Dept. of Earth & Planetary Sciences
Campus Box 1169
Washington University
One Brookings Drive
St. Louis, MO 63130

ph: 314-935-5622
fax: 314-935-7361
blj-at-levee.wustl.edu

http://epsc.wustl.edu/


From daemon Fri Nov 9 08:55:26 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Fri, 9 Nov 2001 09:27:31 -0500
Subject: SEM Service Contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sarah,

I know you are looking for an independent service provider. However, in the
past, I had been very pleased with JEOL service. Currently, I am working on
a Hitachi EM, but for 12 years prior I worked on JEOL scopes (both TEM and
SEM). The lab that I worked in was on the east coast in New Jersey. I'm
not certain how the service is on the west coast, but the number one reason
why I purchased new JEOL EMs was for their service history. We had an old
JSM-35 and wanted to replaced it as well as purchase a new TEM. Based on
the service we had received in the past, we immediately considered JEOL's
line of EMs. Granted, this was about 7 years ago since the purchase and
about 2 years since I've been out of that particular lab; however I was very
pleased with the service from the north east division of JEOL.
Good Luck with your search.

Jackie Garfield



From daemon Fri Nov 9 09:16:19 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Fri, 9 Nov 2001 10:03:13 -0500
Subject: RE:TEM Request for methold for cutting thin sections from thick.

Contents Retrieved from Microscopy Listserver Archives
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Dear Jo Ellen,

I intended on writing you right away, but I got too busy in the lab. I have
tried this procedure a few times in the past with some success. I have two
articles which I can fax to you if you would like. Sorry, I do not have an
electronic copy. The references are:

Gonzalez-Angulo A, Ruiz De Chavez I, Castaneda M: A Reliable Method for
Electron Microscopic Examination of Specific Areas from Paraffin-embedded
Tissue Mounted on Glass Slides. American Society of Clinical Pathologists
(1978) 697-700.


Bretschneider A, Burns W, Morrison A: "Pop-off" Technic. The Ultrastucture
of Parafin-embedded Sections. American Journal of Clinical Pathology. Vol.
76, No. 4, October 1981. 450-453.


Jackie Garfield
Reseach & Development
Lifecell Corporation
One Millennium Way
Branchburg, NJ 08876
(908) 947-1182
jgarfield-at-lifecell.com


From daemon Fri Nov 9 10:03:22 2001



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Fri, 09 Nov 2001 11:01:00 -0500
Subject: OT: possible spectacular Leonid meteors, Nov 18/19

Contents Retrieved from Microscopy Listserver Archives
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Pardon the off-topic post, but this is the only
list to which I subscribe which has a significant
number of members in Australia and East Asia,
where the meteor shower has potential to be
a once or twice in a lifetime experience this
year:

}
} http://science.nasa.gov/headlines/y2001/ast08nov_1.htm?list534615
}

For North America, best watching period is 4AM
to 6AM EST on Nov. 18. For Australia and East
Asia, predicted maximum is in the hours between
midnight and about 4AM, depending on location,
with possible maximum ZHR (zenithal hourly rates)
approaching 10,000 per hour from dark-sky sites.

Astronomy is just like microscopy except we look at
bigger things with bigger instruments, right?

Rick Mott


From daemon Fri Nov 9 10:54:25 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 09 Nov 2001 10:54:35 -0500
Subject: Re: Philips 400T IGP powersupply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


thurston e herricksthurst0n-at-u.washington.edu11/8/01 3:59 PM


Thurston,

FEI is located in Hillsboro, OR.

Contact them at: 1-503-640-7500.

Good luck!

Gib

} Hello,
}
} I am looking for a transformer to go into the Ion getter pump powersupply
} for a Philips 400T. The power supply transformer in this microscope is
} fried and needs to be replaced. I'm not sure who to contact since the
} microscope division of Philips is now owned by FEI. Any information would be
} appreciated.
}
} Take care
}
} Thurston Herricks
--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/




From daemon Fri Nov 9 10:59:47 2001



From: Kevin Mackenzie :      k.s.mackenzie-at-abdn.ac.uk
Date: Fri, 09 Nov 2001 16:55:00 +0000
Subject: TEM of Brown adipose tissue

Contents Retrieved from Microscopy Listserver Archives
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I have been asked to prepare some samples of brown adipose tissue for TEM,
to look at mitochondrial structure and density.
Has anyone got any suggestions for fixative and processing schedule for
this type of material.

thanks

Kevin
Electron Microscope unit
Department of Zoology
University of Aberdeen
Aberdeen
AB24 2TZ

Tel 01224-272847
Fax 01224-272396
------------
Kevin Mackenzie
k.s.mackenzie-at-abdn.ac.uk



From daemon Fri Nov 9 11:07:24 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 9 Nov 2001 08:59:09 -0800
Subject: Re: Ask-A-Microscopist:LM Kohler Illumination Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Question: My 8-year-old daughter and I have developed an interest in
} microscopy as a hobby.
} We have a Meade 9400 student microscope with built-in light (no field
} diaphram) and a fixed condenser (with a rotating aperture wheel).
} I am able to "focus" the condenser by inserting blocks under the
} slide (and lowering the stage to focus).
} This has allowed me to get closer to the critical focusing position
} (the surface of the light source now focuses about 1 cm above the
} stage.
} I need this for an effective dark-field effect at 100x magnification
} (10x10). I have heard that in order to best see thick specimens
} (especially at 400x), we need Kohler illumination.
} Do you think this will really make a difference? If so, what
} modifications would I need to make to achieve this?
} Many thanks.
} ---------------------------------------------------------------------------
Reimar -

You'll find several suggestions for modifying your scope in Stevens' "The
Microscope on a Budget"; here's the description from the MICRO bibliography
(URL below):

Stevens, M.B. 1993 The Microscope on a Budget 247pp, 5.5x8.5",
paperback, $13.00. ISBN 0-9638839-1-7 Logical Image Research, P.O.Box
1523, Buda, TX 78610. Also available from Nasco, 4825 Stoddard Rd.,
Modesto, CA 95356-9318 as #SB23799M; 800-558-9595.
Subtitled "a complete guide to the low cost light microscope for
the laboratory, photographers, and hobbyists", this book delivers solid
information; it's a good buy. Homemade equipment (including exciting but
seldom-discussed things like darkfield and Rhineberg illumination) is
described in detail for the low-budget teacher or (adult-supervised)
student. Both basic biological microtechnique and microcrystal preparation
are described in detail. There are photography, supply source, reference,
glossary, and index sections. High school - adult. RECOMMENDED

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Nov 9 11:50:29 2001



From: Lou Ross :      RossLM-at-missouri.edu
Date: Fri, 9 Nov 2001 11:45:02 -0600
Subject: Microbeam Analysis Society renewal reminder

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Just wanted to send out a reminder about the ballot and M&M Journal
subscription deadlines.

Ballots MUST BE postmarked by December 1, 2001 to be counted.
M&M Journal subscription forms MUST BE returned by December 15, 2001 for
the 2002 subscriptions.

Both the ballots and the subscription forms can be returned with your MAS
renewal application.

If you have any questions, please contact me at rosslm-at-missouri.edu or
(573) 882-4777 or at the the MAS email address or phone number listed below.

Thanks,
Lou Ross
MAS Membership Services
masmembership-at-excite.com
1-800-4MASMEM (1-800-462-7636)
www.microanalysis.org


From daemon Fri Nov 9 15:51:41 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 09 Nov 2001 16:42:42 -0500
Subject: Re: OT: possible spectacular Leonid meteors, Nov 18/19

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


on 11/9/01 11:01 AM, Rick Mott at rickmott-at-alumni.princeton.edu wrote:
}
} Astronomy is just like microscopy except we look at
} bigger things with bigger instruments, right?
}
Dear Rick,
That, and the fact that no one has yet invented electron astronomy
(although study of the solar wind could be called proton astronomy)--let
alone scanning probe astronomy (scanning black-hole force astronomy
anyone?). :-)
Yours,
Bill Tivol



From daemon Fri Nov 9 16:50:14 2001



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Fri, 9 Nov 2001 16:43:28 -0600
Subject: Re: How to sample?

Contents Retrieved from Microscopy Listserver Archives
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Good Day,

Counting the number of simple convex 3-D particles from some 3-D reference space
(i.e. cell) using 2-D sections is not a trivial task. The sectioning process
reduces the dimension of the particles to 2-D in the section. This 2-D sample
of a particle is usually referred to as a profile. Many people have counted the
number of profiles in a section and used this number to estimate the number of
particles in the 3-D reference space. This of course is usually not a very good
estimate of the TRUTH since the number of profiles in the 2-D sample is related
not only to the number of particles but also the volume of the particles (more
correctly the length of the particle perpendicular to the section plane) in the
3-D reference space. As Paul Webster pointed out in his earlier submission,
Hans Gundersen solved the problem of counting particles in sections in his
DISECTOR paper (J Microscopy 134:127-136, 1984).

Determining the number of complex particles, such as branching mitochondria adds
complexity to the counting task. Luckily the hypothetical graduate student in
Fred Monson's example did not take her EM class last year since Gundersen only
solved the problem of easily counting complex particles using 2-D sections in
September 2001 (J Microscopy 203:314-320). In this paper he introduces the
ConnEulor principle for counting complex particles. Coincidentally the example
he uses to demonstrate the ConnEulor principle is to determine the number of
mitochondria in a cell. This technique like the original disector technique
gives a density measure (number/volume) and you must still multiply by the
average cell volume to obtain your estimate of particle number per cell.

A practical grad student might wonder if number of mitochondria is necessary and
decide to measure the total volume of mitochondria within the cell (a much
easier parameter to measure).

John


}
} Hi Fred,
}
} At the risk of looking very stupid, I will take your challenge.
}
} Am I correct in understanding that your question has two parts? 1. What is
} the mean number of mitochondria per yeast cell in a population. 2. What is
} the mean area of cristae membranes.
}
} If this is so, then there are actually three challenges here because to get
} an absolute number for the cristae surface area and the number of
} mitochondria per cell we also need to have an estimate for a reference space
} in actual numbers. A good reference space to estimate is cell volume.
}
} Obtaining mean number of mitochondria per cell is actually very easy. I
} would first throw away the images already obtained because they were
} subjectively sampled.
}
} We must then remove bias that may be a result of centrifugation - heavier
} (more dense) cells will pellet to the bottom, with the possibility that a
} gradient is formed in the pellet. To avoid this, embed the cells first in
} fast-setting gelatin or agarose, being careful to make sure the cells have
} been thoroughly mixed. We could of course do it properly and apply the
} "orintator" to make sure our sampling is isotropic (Mattfeldt et al 1989
} Acta Stereol. 8:671-676).
}
} Fix the block and cut it into slabs, rods and then cubes. Using a
} sequential random sampling protocol, take every "nth" block (decided using
} random number generator) and embed them separately in resin (see Lucucq 1993
} Trends Cell Biol 3:345-358 for details).
}
} Section for counting. A good way to sample the cells to count particles
} (here is where the catch comes in) is to only count the tops of the
} particles. With the yeast mitochondria, the 2-D profile does not always
} represent an individual particle. A profile may represent one single
} mitochondrion, or may represent a multiply branched, single mitochondrion,
} depending on the level of branching that is occurring. By counting the
} tops, connected profiles are eliminated from the sampled population. (for
} details on a possible method for estimating the amount of branching that is
} present, look for "Star Volume" in Gundersen et al 1986 J. Microsc.
} 143:3-45)
}
} How to sample? Use the "Disector" (in this instance, the word means "2
} sectors"). The reference is D.C. Stereo (a pseudonym for HJG Gundersen)
} 1984. J. Microsc. 134:127-136.
}
} To apply, cut two sequential sections and take similar fields of view from
} both. Use one field to examine for mitochondrial profiles and the other to
} check if the profile is also present there ( the look-up frame). If a
} profile is present in one field but not present in the other, it is counted
} as a "top". (It is a variation of the 2-D counting rule, the associated
} tangent rule).
}
} Knowing the area of the field of view and the thickness separating the two
} sequential sections, we can work out the volume being sampled. This will
} give us an estimate of the number of mitochondria per unit volume. This is
} not the number of mitochondria per cell. We still need to estimate the cell
} volume.
}
} To estimate surface area, we can use simple cross lattice overlays and count
} the number of times a membrane profile crosses a test line. From this we
} can estimate length of membrane, and by knowing section thickness, can
} obtain an estimate of membrane area. (see H.J.G. Gundersen et al 1986 J.
} Microsc. 143:3-45. 1988 APMIS 96:379-394 & 857-881, T.M. Mayhew 1991 Exp.
} Physiol. 76:639-663, J. Lucocq for details on how to do this) We still need
} to estimate the cell volume.
}
} How am I doing so far, Fred?
}
} Now, a question for you. I can provide estimates of relative values for
} number and surface area. However, I do need a reference space to obtain
} absolute values. How would I obtain a reliable estimation of the mean cell
} volume of the yeast population? Presumably they are a rapidly dividing
} population.
}
} Regards
}
} Paul Webster.
}
}
}
}
}
}
} on 11/7/01 12:53 PM, Monson, Frederick C. at fmonson-at-wcupa.edu wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hypothetical!
} }
} } A graduate student in microbiology has just finished her first course in
} } electron microscopy, and she informs her advisor that she wants to study
} } mitochondrial structure and function in several related strains of yeast,
} } for one of which she fortuitously has electron micrographs of mitochondrial
} } profiles that are, she has been told, quite publishable.
} }
} } Her advisor suggests that she start by comparing 3-4 strains with respect
} } to
} } number of mitochondria per cell and total mitochondrial cristal area under
} } identical conditions of culture.
} }
} } The student happily responds that she already has micrographs for regularly
} } sampled sections from serial sections she learned to make of the strain she
} } used in her EM course. She returns the next week with estimates of cristal
} } area and numbers of mitochondria per cell based on morphometric analysis of
} } those regularly sampled sections.
} }
} } If the cell is 12-25um in length (haploid and diploid) and always between
} } 3-5um in diameter, and if the mitochondrial profiles are in the range of
} } 1-2um (circular and ovoid, and sometimes or often branched), what should
} } the sampling criteria be for cells that are serially sectioned with
} } sections
} } approximately 90nm in thickness?
} }
} } How could one derive the same, but apparently much more statistically
} } satisfactory, data from the 40+ cell profiles per section of cells randomly
} } oriented and not necessarily (or importantly) sectioned or analyzed
} } completely.
} }
} } All who take on this task should know there is an extremely important, and
} } possibly significant, hint that can be observed at:
} } http://www.msg.ucsf.edu/sedat/marsh/mito.html. I sometimes think it is
} } well
} } to know of such hints (or at least to be aware of their possibility) before
} } attempting a task. Assumptions are almost always incorrect unless
} } rigorously defined and applied, and very often difficult to prove as such
} } or
} } to isolate in generating an experimental design without flaws.
} }
} } Knowing that reality often gets in the way of statistics, and vice versa, I
} } am
} }
} } Yours most respectfully, because I wrestle with this problem every once in
} } a
} } while and I haven't yet determined a satisfactory answer.
} }
} } Fred Monson
} }
} } Frederick C. Monson, PhD
} } Center for Advanced Scientific Imaging(CASI)
} } West Chester University of Pennsylvania
} } Schmucker Science Center II
} } CASI Home Page: http://darwin.wcupa.edu/casi/
} } South Church Street
} } West Chester, PA, 19383
} } Office: SS024
} } Phone: 610-738-0437
} } FAX: 610-436-3036
} } eMail: fmonson-at-wcupa.edu
} } Please call before visiting
} }
} }
}
}
}
} .


John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Sun Nov 11 17:01:39 2001



From: Mark G BLACKFORD :      mgb-at-ansto.gov.au
Date: Mon, 12 Nov 2001 09:36:11 +1100
Subject: RE: possible spectacular Leonid meteors, Nov 18/19

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,

the much anticipated Leonid meteor show next Monday morning (Australian
time) promises to be a spectacular event if the various predictions are even
half correct. Unfortunately there are no guarantees (unlike eclipse
predictions) so those of us making travel plans to observe the Leonids risk
being in the wrong place at the wrong time. Personally I think the risk is
worth taking considering the spectacular sight of thousands of meteors per
hour if things work out.

In 1999 I drove for 5 hrs west of Sydney with a friend and my son in an
attempt to observe a predicted reasonable Leonid shower. As it turned out
we were treated to a much closer light show. The most ferocious thunder
storm for twenty years devastate the local orchards in the area we chose to
observe from and chased us all the way back to Sydney. We did manage to see
a few dozen bright meteors through patchy clouds early in the night so it
wasn't a total loss astronomically speaking.

This year I will be observing from central Australia, just a few kilometers
from Ayres Rock. I figure that even if the sky show is clouded out or the
predictions don't hold up, at least I can get a few good bush walks done
during the days.

I'll give a brief report next week. Cheers,


Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.


} ----------
} From: Rick Mott
} Sent: Saturday, November 10, 2001 3:01 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: OT: possible spectacular Leonid meteors, Nov 18/19
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Pardon the off-topic post, but this is the only
} list to which I subscribe which has a significant
} number of members in Australia and East Asia,
} where the meteor shower has potential to be
} a once or twice in a lifetime experience this
} year:
}
} }
} } http://science.nasa.gov/headlines/y2001/ast08nov_1.htm?list534615
} }
}
} For North America, best watching period is 4AM
} to 6AM EST on Nov. 18. For Australia and East
} Asia, predicted maximum is in the hours between
} midnight and about 4AM, depending on location,
} with possible maximum ZHR (zenithal hourly rates)
} approaching 10,000 per hour from dark-sky sites.
}
} Astronomy is just like microscopy except we look at
} bigger things with bigger instruments, right?
}
} Rick Mott
}
}


From daemon Mon Nov 12 07:35:16 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Mon, 12 Nov 2001 08:20:58 -0500
Subject: RE: Ask-A-Microscopist:LM Kohler Illumination Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-----Original Message-----
} From: reimar_gaertner-at-wsib.on.ca [mailto:reimar_gaertner-at-wsib.on.ca]
Sent: Thursday, November 08, 2001 8:19 PM
Hi Reimar, Here's a cheap substitute for a Kohler type illumination I
learned early in my microscopy career. Put a piece of translucent tape
(magic mending type) on the rear of the slide. While this is not Kohler it
comes close as it mimics the effect of light emitted close to the sample.
The thinner the slide the better e.g. a cover slip. Good luck and have fun.
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


Email: reimar_gaertner-at-wsib.on.ca
Name: Reimar Gaertner

Organization: WSIB

Education: Graduate College

Location: Toronto, Ontario, Canada

Question: My 8-year-old daughter and I have developed an interest in
microscopy as a hobby.
We have a Meade 9400 student microscope with built-in light (no field
diaphram) and a fixed condenser (with a rotating aperture wheel).
I am able to "focus" the condenser by inserting blocks under the
slide (and lowering the stage to focus).
This has allowed me to get closer to the critical focusing position
(the surface of the light source now focuses about 1 cm above the
stage.
I need this for an effective dark-field effect at 100x magnification
(10x10). I have heard that in order to best see thick specimens
(especially at 400x), we need Kohler illumination.
Do you think this will really make a difference? If so, what
modifications would I need to make to achieve this?
Many thanks.
Reimar

---------------------------------------------------------------------------


From daemon Mon Nov 12 08:25:27 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Mon, 12 Nov 2001 08:56:54 -0500
Subject: old JSM-35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Morris,

Unfortunately, we used our old JSM-35 as a trade-in. The purchase was about
7 years ago. The scope was in good shape, so I am sure it got a good home.
However, you can check with JEOL for any used scopes. With a little luck,
they just might have something. Also, you may want to check with Dick
Daniel from Radco, Inc. (201) 891-8647. He services quite a bit of SEM
equipment (mostly Denton products), and has many contacts throughout the
United States. Perhaps, he knows of a good used SEM. It is worth a call.
Good Luck,

Jackie Garfield


From daemon Mon Nov 12 15:57:17 2001



From: David E. Luzzi :      luzzi-at-lrsm.upenn.edu
Date: Mon, 12 Nov 2001 16:47:26 -0500
Subject: Multiple Post-doctoral Fellowships in Nanoscience

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nanotubes and Peapods - Synthesis, Processing, Structure, Properties

Great progress has been made in the synthesis and characterization of the
new class of materials now commonly known as peapods – 1-D chains of
molecules contained within carbon nanotubes - discovered in our group at
Penn in 1998. These materials hold great promise as new nanoscale components
with new functionality. Their are several openings in the Luzzi research
program on a number of ongoing projects in the design, production and
characterization of peapod systems and related work. The experimental
program necessarily includes work with carbon nanotubes in the
as-synthesized, pre-filled state. As this field is early in its development,
the opportunities to work and make an impact are wide-open and far-reaching.

The experimental program involves electron microscopy including in-situ
experiments, chemical synthesis and processing, and physical property
measurements. The successful candidate should work well in a group, but be
interested in developing and driving an aggressive, independent research
effort.

The positions include health benefits and a competitive salary. We follow
the University of Pennsylvania equal-opportunity guidelines and policies. If
you would like to apply, please send a CV and the contact information of
your references to David Luzzi at

luzzi-at-lrsm.upenn.edu



From daemon Mon Nov 12 15:57:17 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 12 Nov 2001 16:52:47 -0500
Subject: Re: Ask-A-Microscopist:LM Kohler Illumination Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mr. Russ,

Be aware, however, that most tape is anisotropic, subject to strain
polarization, and will have a slight polarizing effect on transmitted
light, perhaps preventing one from obtaining complete extinction between
crossed polars or otherwise modifying expected optical effects.

John Twilley
Conservation Scientist

Gillmeister, Russ wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} -----Original Message-----
}
} } From: reimar_gaertner-at-wsib.on.ca [mailto:reimar_gaertner-at-wsib.on.ca]
}
} Sent: Thursday, November 08, 2001 8:19 PM
} Hi Reimar, Here's a cheap substitute for a Kohler type illumination I
} learned early in my microscopy career. Put a piece of translucent tape
} (magic mending type) on the rear of the slide. While this is not Kohler it
} comes close as it mimics the effect of light emitted close to the sample.
} The thinner the slide the better e.g. a cover slip. Good luck and have fun.
} Russ Gillmeister
} Xerox
} ~~~~~~~~~~~~~
}
}
} Email: reimar_gaertner-at-wsib.on.ca
} Name: Reimar Gaertner
}
} Organization: WSIB
}
} Education: Graduate College
}
} Location: Toronto, Ontario, Canada
}
} Question: My 8-year-old daughter and I have developed an interest in
} microscopy as a hobby.
} We have a Meade 9400 student microscope with built-in light (no field
} diaphram) and a fixed condenser (with a rotating aperture wheel).
} I am able to "focus" the condenser by inserting blocks under the
} slide (and lowering the stage to focus).
} This has allowed me to get closer to the critical focusing position
} (the surface of the light source now focuses about 1 cm above the
} stage.
} I need this for an effective dark-field effect at 100x magnification
} (10x10). I have heard that in order to best see thick specimens
} (especially at 400x), we need Kohler illumination.
} Do you think this will really make a difference? If so, what
} modifications would I need to make to achieve this?
} Many thanks.
} Reimar
}
} ---------------------------------------------------------------------------
}
}
}



From daemon Mon Nov 12 19:55:58 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 13 Nov 2001 14:39:33 GMT+1200
Subject: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi

We have a Nikon Labophot-POL microscope.

In its trinocular head there sits a CF PL 5X Projection lens, above
that is a Microflex HFX-IIA Photomicrographic Attachment, and onto
that is a "35mm camera adapter A", then what is called in the HFX-IIA
manual a "Motorized dark box FX-35WA". The latter looks more like a
camera body to me.

We want to be able to mount our Nikon D 1 body onto the microscope,
with or without the HFX-IIA, whatever it takes.

Is there anyone out there who can tell, from the above, what I need
to do to acheive this?

I would have thought that all we need to do would be to mount the D 1
body directly onto the trinocular vertical tube, but then I know
almost nothing about light microscopy.

tia

rtch


Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Nov 12 19:55:59 2001



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Mon, 12 Nov 2001 17:43:10 -0800
Subject: LKB Knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

Does anyone know who services or can tell me about how the mechanism
for the LKB knifebreaker works? The knob that you turn to put
pressure on the glass to break is very difficult to turn. Is there
an easy way to clean or free it up? Will some solvent work or WD40?
Or is there something else that I can do?

Thanks in advance.

Mei Lie
--
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu


From daemon Tue Nov 13 08:44:39 2001



From: tartenon-at-netscape.net
Date: Tue, 13 Nov 2001 09:32:33 -0500
Subject: RE: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr Sims, first of all you need to get a C-Mount for the microscope, at the bottom of the trinocular head there are 3 hex-screw use them to remove the tube and insert the c-mount with your D1 camera, connect all the wires on the D1, and enjoy.

Alfredo Hernandez
"Ritchie Sims" {r.sims-at-auckland.ac.nz} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


__________________________________________________________________
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From daemon Tue Nov 13 08:44:39 2001



From: Kelly Stanard :      kstanard-at-ncifcrf.gov
Date: Tue, 13 Nov 2001 09:36:20 -0500
Subject: Balzers Freeze-fracture unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My name is Kelly Stanard and I am a lab tech in the Image Analysis
Laboratory at National Cancer Institute at Frederick, Maryland. I
have been left with a Balzers freeze-fracture unit and some basic
instructions but I have no idea what I am doing. Although a few of
my attempts have produced decent replicas, I am very uncomfortable
with the unit. What I am in need of are a few guidelines as to what
all the buttons are for and when would be a good time to push them!!
What I believe I have sitting here is a High Vacuum Coating Unit BAF
300 purchased in May, 1976.
Quartz Crystal Thin Film Monitor QSG 201
Freeze Etching Unit Control BMS 101
Commutator Unit BCM 101
Pumping Unit Control DPA 101
Control Unit EVM 052A
Microtom Movement Control BMB 101
Chamber IKR 010

If you can help me in anyway it would be greatly appreciated.

Thank you,

Kelly Stanard
Image Analysis Laboratory
NCI- Frederick
P.O. Box B
Frederick, MD 21702
301-846-1528
kstanard-at-ncifcrf.gov


From daemon Tue Nov 13 08:44:39 2001



From: tartenon-at-netscape.net
Date: Tue, 13 Nov 2001 09:38:40 -0500
Subject: RE: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr Sims, first of all you need to get a C-Mount for the microscope, at the bottom of the trinocular head there are 3 hex-screw use them to remove the tube and insert the c-mount with your D1 camera, connect all the wires on the D1, and enjoy.

Alfredo Hernandez

"Ritchie Sims" {r.sims-at-auckland.ac.nz} wrote:

} ------------------------------------------------------------------------
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__________________________________________________________________
Your favorite stores, helpful shopping tools and great gift ideas. Experience the convenience of buying online with Shop-at-Netscape! http://shopnow.netscape.com/

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From daemon Tue Nov 13 09:56:14 2001



From: Jacqueline D. Garfield :      JGarfield-at-lifecell.com
Date: Tue, 13 Nov 2001 10:41:38 -0500
Subject: RE: LKB Knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mei Lie,

You can try calling Leica for service, however you may have to send the
knife-maker out. I use a company called Dolbey-Jamison, located in PA.
They do in-house service.
Good Luck,

Jackie Garfield
Lifecell Corp.


From daemon Tue Nov 13 10:17:46 2001



From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 13 Nov 2001 11:09:43 -0500
Subject: Range of size of neuronal nuclei

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know off the top of their head what the range of size of
neuronal nuclei is?

Cheri Owen
Dept. Emergency Medicine/Physiology
Wayne State University



From daemon Tue Nov 13 11:22:20 2001



From: l.tetley :      l.tetley-at-bio.gla.ac.uk
Date: Tue, 13 Nov 2001 17:13:24 +0000
Subject: UK Cryomicroscopy Group Meeting

Contents Retrieved from Microscopy Listserver Archives
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CRYOMICROSCOPY GROUP ANNUAL MEETING
EM Centre, University of Birmingham, Birmingham, UK

Wednesday 21st November 2001

PROGRAMME :

9.30 - 10.30
Registration, Coffee and Trades Exhibition
10.30
Chairman's Welcome and Introduction
10.35 -11.15
Charge elimination for the X-ray
microanalysis of frozen
hydrated specimens
Patrick Echlin, Cambridge Analytical
Microscopy, Cambridge
11.15 -12.00
Freeze-substitution and 3D reconstruction of
muscle
Pradeep Luther, Imperial College, London
12.00 -12.45
Silver enhancement for immunolabelling
Jan Leunissen, Aurion, Wageningen, The
Netherlands
12.45 - 14.00
Lunch , Trades Exhibition & Posters
14.00 -14.15
Annual General Meeting
14.15 - 15.00
RMS Beginners Competition
15.00 -15.45
Ultrastructural localisation of Prion
protein in the brain using
cryo-immunogold electron microscopy
Peter Peters, Netherlands Cancer Institute,
Amsterdam
15.45 - 16.00
Problems with preparing articular cartilage
for microscopic
study
Iolo ap Gwynn, S C Wade and R G Richards.
University of
Wales, Aberystwyth
16.00
Closing Remarks and Tea

FURTHER DETAILS : http://www.cryomicroscopygroup.org.uk/index.htm


Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel 0141 330 4431
FAX 0141 330 3516

Integrated Microscopy Facility:
http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


From daemon Tue Nov 13 13:53:31 2001



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Tue, 13 Nov 2001 14:44:23 -0500
Subject: TEM of brown adipose tissue

Contents Retrieved from Microscopy Listserver Archives
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Use acetonitrile instead of ethanol or acetone for dehydration. Also, use
imidazole buffered osmium to postfix. The mitochondria come out
beautifully. I have done this and it works. The references are:

Edwards, H. H. et al. 1992. Microscopy Research and Technique Volume 21,
pp 39-50.

Angermuller, S. and H. D. Fahimi. 1982. Histochemical Journal Volume 14,
pp. 823-835.

Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Tue Nov 13 18:54:04 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 13 Nov 2001 19:46:00 -0500
Subject: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I am expecting the following samples for paraffin embedding sometime
soon -- "wolf ovaries have been preserved in tequila for the past 6 months
or so". I first assumed that this was a joke but apparently they are
serious--this is a reproductive physiology project from an ecology
lab. Any comments are welcome--levity included. I can hear your laughter
already! My questions:
--has anyone used tequila as a fixative?
-- is the embedding worth pursuing?
Rosemary Walsh



From daemon Tue Nov 13 20:16:30 2001



From: Kalman Rubinson :      kr4-at-nyu.edu
Date: Tue, 13 Nov 2001 21:13:20 -0500
Subject: Re: Range of size of neuronal nuclei

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cheri Owen wrote:

} Does anyone know off the top of their head what the range of size of
} neuronal nuclei is?

Off hand: 5-18 microns.

Kal


From daemon Tue Nov 13 21:07:39 2001



From: snoringremedy-at-excite.com
Date: Tue, 13 Nov 2001 22:11:28 -0500
Subject: LKB Knifebreaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We service and sell reconditioned LKB Knife Makers.
Please contact me off line.
We are closed from Nov. 15 to 26.
Regards,
Microscopy Labs
P.O.Box 338
61 West Street
Red Bank, NJ 07701
732 727 6228
fax 732 758 9142


----- Original Message -----
} From: "Mei Lie Wong" {wong-at-msg.ucsf.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 12, 2001 8:43 PM


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From daemon Wed Nov 14 02:41:51 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 14 Nov 2001 09:41:04 +0100
Subject: Re: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No experience and can't think of any jokes but .....

I guess tequila is about 40% ethanol or is this home-brewed stuff??
Standardisation in the future could be a problem - will they be using the
same brand next time?

Limited lipid extraction in that case - wash in PBS and cryosection?

You could rehydrate to PBS and refix in buffered formaldehyde - could help
to protect against processing.

I guess that wolf ovaries are resonably large so you could try various
combinations?

I would be interested to hear how it goes. Go for it and educate us all!



At 19:46 2001-11-13 -0500, Rosemary Walsh wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Wed Nov 14 07:49:24 2001



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Wed, 14 Nov 2001 10:09:59 -0330
Subject: RE: Nikon camera-microscope interfacing

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} -----Original Message-----
} From: "tartenon-at-netscape.net"-at-sparc5.microscopy.com

} Dr Sims, first of all you need to get a C-Mount

The only question now is which C-mount. Whereas the CCD for Coolpix
mounts requires 1X, the CCD for the D1 is larger ... 1.5X, 2X???

shAf :o)



From daemon Wed Nov 14 08:39:04 2001



From: gary.m.brown-at-exxonmobil.com
Date: Wed, 14 Nov 2001 08:33:09 -0600
Subject: Re: a sample prep queri

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Rosemary,

I haven't worked in biological EM for some time. I do, however, have some
experience with the stated topic. One evening some years ago I partook a
bit too much tequila and had a howling good time. Unfortunately, the next
morning I found that I was very well fixed indeed. Needed the hair of the
dog (or wolf as the case may be) to survive the day. That memory has been
well embedded in my memory as I wax nostalgic.

I have no other comments aside from this nonsense. Good luck.

Gary M. Brown




Rosemary Walsh
{rw9-at-psu.edu} To: Microscopy-at-sparc5.microscopy.com
cc:
Subject: a sample prep queri
11/13/01 06:46
PM





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Dear Listers,
I am expecting the following samples for paraffin embedding
sometime
soon -- "wolf ovaries have been preserved in tequila for the past 6 months
or so". I first assumed that this was a joke but apparently they are
serious--this is a reproductive physiology project from an ecology
lab. Any comments are welcome--levity included. I can hear your laughter
already! My questions:
--has anyone used tequila as a fixative?
-- is the embedding worth pursuing?
Rosemary Walsh








From daemon Wed Nov 14 08:47:52 2001



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 14 Nov 2001 09:38:08 -0500
Subject: resins for culture dishes and plates

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,
Some time ago one of you discussed a combination of resins that snapped off
culture dishes fairly easily. Could you post that again as I need the
information and can't seem to locate it in the archives. I've tried
several combinations of resins from different sources on different brands
of plates and the results are not great. There also appear to be
differences in the "snapability" between the multiple well plates and the
individual dishes from the same companies, which adds to the problem. I'd
really appreciate knowing the magic combination of resin components if you
could readdress the issue.
Thanks,
Mary Gail Engle

Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
Health Sciences Research Bldg. 001
University of Kentucky
Lexington, KY 40536-0305

phone 859-323-6108
fax 859-257-9700


From daemon Wed Nov 14 09:06:48 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 14 Nov 2001 10:02:24 -0500
Subject: Re: a sample prep queri

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} --has anyone used tequila as a fixative?
} -- is the embedding worth pursuing?
} Rosemary Walsh
******************
Hi Rosemary,
that's a new one.

My first question is, what proof was the tequila? If it was potent
stuff, the ethanol level may have been high enough to act as a
coagulative fixative. Many years ago I was asked to do EM of the
gills of clams that had been fixed in 70% ethanol. I rehydrated them
to buffer and then processed for EM as usual (glut, osmium, etc).
The ultrastructure, while clearly compromised was far better than I
had expected. You can try this with the ovary samples. The fact
that they are destined for paraffin means that you won't have the
very fine structure to worry about. You might want to use a higher
than usual concentration of pfa, or even throw in a little glut to
lock in whatever is left.

Good luck,
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Nov 14 09:06:49 2001



From: Michelle.Taurino-at-aventis.com
Date: Wed, 14 Nov 2001 09:00:11 -0600
Subject: Luxol Fast Blue

Contents Retrieved from Microscopy Listserver Archives
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Hello fellow microscopists-

Does anyone have any experience with staining thick (semi-thin) sections
with Luxol Fast Blue?

Any recommendations or thoughts are appreciated.

Thank you-
Michelle Taurino

Michelle Taurino
Aventis Pharmaceuticals
Senior Scientist
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com



From daemon Wed Nov 14 09:16:06 2001



From: Gruber, Tyler :      tgruber-at-phelpsd.com
Date: Wed, 14 Nov 2001 10:10:31 -0500
Subject: Cryosectioning services desired

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Listers,

I would like to contract out cryosectioning of some carbon black - filled rubber material. We desire sections on the order of 1 micron thick amenable to light microscope/image analysis measurements that we will perform. There is plenty of sample material and we don't need serial sectioning. We are hoping to have this done within weeks.

If you are interested or know of someone who may be, and/or have questions, please contact me off line at tgruber-at-phelpsd.com.

Thanks,

Tyler C. Gruber



From daemon Wed Nov 14 11:27:47 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 14 Nov 2001 12:16:00 -0500
Subject: RE: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
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Not too dumb on the part of the enviro's. After all, Tequila does the worm!
Tequila, as I quickly discovered, can have between 35-55% ethanol.

My recommendation would be to proceed with your own fixation and not worry.
They used their heads when they realized they went into the field
unprepared, or they had an accident, or they couldn't take the fixative with
them and couldn't find any there. You treat the tissue as if it just got to
you. The nitrogens are likely still waiting for the formaldehyde. Or, you
could transfer them to Clarke's 3:1(Absolute ethanol:Glacial Acetic Acid),
and have a really granular texture in the sections. Or Bouin's, which would
also complete the job on the worm!

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/



} ----------
} From: Rosemary Walsh
} Sent: Tuesday, November 13, 2001 7:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: a sample prep queri
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} I am expecting the following samples for paraffin embedding sometime
}
} soon -- "wolf ovaries have been preserved in tequila for the past 6 months
}
} or so". I first assumed that this was a joke but apparently they are
} serious--this is a reproductive physiology project from an ecology
} lab. Any comments are welcome--levity included. I can hear your laughter
}
} already! My questions:
} --has anyone used tequila as a fixative?
} -- is the embedding worth pursuing?
} Rosemary Walsh
}
}
}


From daemon Wed Nov 14 11:27:47 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 14 Nov 2001 12:18:31 -0500
Subject: RE: Range of size of neuronal nuclei

Contents Retrieved from Microscopy Listserver Archives
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As I recall the lower range is around that for normal cells and the upper
range is something like 15-40um (BIG!!!). Just hope that J. Kiernan sees
your email so that you get a more refined result.

Fred Monson


} ----------
} From: Cheri Owen
} Sent: Tuesday, November 13, 2001 11:09 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Range of size of neuronal nuclei
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
}
} Does anyone know off the top of their head what the range of size of
} neuronal nuclei is?
}
} Cheri Owen
} Dept. Emergency Medicine/Physiology
} Wayne State University
}
}
}


From daemon Wed Nov 14 12:37:18 2001



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Wed, 14 Nov 2001 13:24:05 -0500
Subject: Position OPEN

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Position Open
Microscopy and Microanalysis Research Assistant


The NCSU Analytical Instrumentation Facility (AIF) is seeking qualified
applicants for a SEM Microscopist position opening December 11, 2001.

Duties and responsibilities include: Operation and maintenance
of SEM/EDS instrumentation and sample preparation and analysis equipment;
scheduling of access to and oversight of the above instrumentation; user
training and assistance; assistance with the teaching of electron microscopy
laboratory classes, and analysis of a wide variety of samples.
Qualifications must include a minimum of a BS with 5 years hands on SEM/EDS
experience, MS with 2 years hands on SEM/EDS experience or a Ph.D. with 1
year hands on SEM/EDS experience. Experience must be in a non biological
materials related discipline. Required skills include: extensive hands on
experience with SEM and related techniques and accessories (e.g. EDS,
specimen preparation and associated analytical tools). Preferred
qualifications include: teaching and user training; familiarity with modern
electronics; and experience with vacuum systems.

Please send resume and three letters of reference to: Phil
Russell, Director; Analytical Instrumentation Facility; North Carolina State
University; Box 7531, Room 318A EGRC; 1010 Main Campus Drive; Raleigh, NC
27695-7531 or email PRUSSELL-at-NCSU.EDU.

North Carolina State University is an Equal Opportunity and Affirmative
Action Employer. ADA Accommodations: Phil Russell, prussell-at-ncsu.edu,
919-515-7501. In its commitment to diversity and equity, North Carolina
State University seeks applications from women, minorities, and persons with
disabilities

______________________________
Roberto Garcia
NCSU/Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh NC 27695-7531
P: (919) 515-8628
F: (919) 515-6965
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm



From daemon Wed Nov 14 13:17:41 2001



From: Jensen, Karen :      Karen_Jensen-at-urmc.rochester.edu
Date: Wed, 14 Nov 2001 13:56:06 -0500
Subject: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
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Rosemary:

It's possible, that the ovaries are fixed OK, provided they were cut open
when placed into the tequila. Normally, 70% ethanol would have been better,
maybe they should have used for alcohol fixation. If they have not been
cut open prior to fixation, then the odds are that the morphology of the
internal areas of the ovary will not be very good. However, try one sample,
trim to desired sized and post-fix in 10% formalin overnight, process for
paraffin embedding.

Good Luck!

Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642




-----Original Message-----
} From: Rosemary Walsh [mailto:rw9-at-psu.edu]
Sent: Tuesday, November 13, 2001 7:46 PM
To: Microscopy-at-sparc5.microscopy.com


Dear Listers,
I am expecting the following samples for paraffin embedding sometime

soon -- "wolf ovaries have been preserved in tequila for the past 6 months
or so". I first assumed that this was a joke but apparently they are
serious--this is a reproductive physiology project from an ecology
lab. Any comments are welcome--levity included. I can hear your laughter
already! My questions:
--has anyone used tequila as a fixative?
-- is the embedding worth pursuing?
Rosemary Walsh



From daemon Wed Nov 14 13:21:00 2001



From: treese :      treese-at-marinebio.mbl.edu
Date: Wed, 14 Nov 2001 13:43:22 -0600
Subject: Balzers Freeze-fracture unit

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My name is Kelly Stanard and I am a lab tech in the Image Analysis
Laboratory at National Cancer Institute at Frederick, Maryland. I
have been left with a Balzers freeze-fracture unit and some basic
instructions but I have no idea what I am doing. Although a few of
my attempts have produced decent replicas, I am very uncomfortable
with the unit. What I am in need of are a few guidelines as to what
all the buttons are for and when would be a good time to push them!!
What I believe I have sitting here is a High Vacuum Coating Unit BAF
300 purchased in May, 1976.
Quartz Crystal Thin Film Monitor QSG 201
Freeze Etching Unit Control BMS 101
Commutator Unit BCM 101
Pumping Unit Control DPA 101
Control Unit EVM 052A
Microtom Movement Control BMB 101
Chamber IKR 010

If you can help me in anyway it would be greatly appreciated.

Thank you,

Kelly Stanard
Image Analysis Laboratory
NCI- Frederick
P.O. Box B
Frederick, MD 21702
301-846-1528
kstanard-at-ncifcrf.gov


From daemon Wed Nov 14 14:37:29 2001



From: George Laing :      scisales-at-ngscorp.com
Date: Wed, 14 Nov 2001 15:26:51 -0500
Subject: RE: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
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The CCD on the D1 is 23.7mm x 15.6mm, so a projection lens of 1.5x should
be sufficient.
Do you have some kind of C-mount to Nikon mount converter? The D1 uses the
same Nikon "F" mount that Nikon SLR bodies have used for years, so without a
converter it will not mount directly to a C-mount.

George

George Laing
National Graphic Supply


The only question now is which C-mount. Whereas the CCD for Coolpix
mounts requires 1X, the CCD for the D1 is larger ... 1.5X, 2X???

shAf :o)





From daemon Wed Nov 14 15:14:20 2001



From: tartenon-at-netscape.net
Date: Wed, 14 Nov 2001 16:07:42 -0500
Subject: RE: RE: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
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C-Mount is just an adapter and wont requiere any additional lens, You'll get a direct image. You can use different c-mounts with zoom in them in order to get a larger field of view. The lens you require will depend on the chip size of your camera (DN100 has 1/2" chip size) so if you want a larger field of view you probably want to buy a C mount + TV adapter 0.45X The DXM1200 has a 2/3" chip size therefore the best combination will be C mount + TV adapter 0.6X

Alfredo
"michael shaffer" {rarewolf-at-roadrunner.nf.net} wrote:

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From daemon Wed Nov 14 19:53:55 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 14 Nov 2001 20:49:14 -0500
Subject: To tequila or not?

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Hi folks,
Thanks for the replies. Apparently this was serious inquiry. Samples
were taken in a remote area in Alaska. The interest is in determining the
number of ovulations compared with the number of pregnancies in dominant
wolves versus pseudo pregnancies in other pack females. We'll probably
rehydrate to PBS and fix in 4% paraformaldehyde, process and embedd in
paraffin, stain with H&E for LM to see what we have. Another option is to
go directly to 10% formalin. If I can obtain some sample I intend to
process for TEM "just for the record". This should take us awhile but I
promise to keep you posted. Thanks again to all of you wonderful folks who
responded. Not yet destined for Margaritaville!
Rosemary


Here are the replies
No experience and can't think of any jokes but .....

I guess tequila is about 40% ethanol or is this home-brewed stuff??
Standardisation in the future could be a problem - will they be using the
same brand next time?Limited lipid extraction in that case - wash in PBS
and cryosection?
You could rehydrate to PBS and refix in buffered formaldehyde - could help
to protect against processing. I guess that wolf ovaries are resonably
large so you could try various combinations?
Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

Sheesh! What an ecology lab. Were they getting the wolves drunk and then
ripping out their ovaries and plunking them in tequila? I've never tried
preserving anything in tequila, but it probably works for livers. Good luck
(and you should charge these idiots A LOT).
Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


Hi Rosemary,
After 6 months or so, do you suppose that the ovaries are
"Wasting Away in Margaritaville"?
JME
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA
phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca

In "The collection and preservation of Animal Parasites" by G.O.W. Kruse e
M.H. Pritchard (Tech. Bull. no 1, The Harold W. Manter Laboratory-
University of Nebraska Press, 1982) the use of any available local brew to
fix and preserve parasites is reported, if no fixative is
available.Transfer of parasites to 70% EtOH as soon as the latter is
available, after carefully pouring out the spirit.Bruno Dore

I have been present at the AFIP when they got specimens similar to your wolf
ovaries. Some of us wanted to go do field work with the guys who came up
with this as they said they use tequila alot, for many things. Our
imaginations went active. You might try contacting the Histology laboratory
there and find out what they do exactly. I can give you some advise a
fellow histologist and I will forward yourmessage to HistoNet as someone
there may be able to assist you also. The first issue is the fixation, at
this point both fixation and dehydration are
complete. Do you plan to dissect the specimens into blocks, if so what
sizes? I have a protocol using butanol to clear the specimen prior to
infiltration with paraffin. It is very gentle and would hopefully allow the
perimeters to stay softer and easier to section. Let me know if I can
assist you or you need the protocol. I am generally in from 9AM to 5PM.
Today will be the exception we have meetings until 1PM.

Pamela A. Marcum
Histology/Microscopy
Product Development Manager
400 Valley Road
Warrington, PA 18976
Phone: 800-523-2575 Ext 167
215-343-6484 Ext 167
Fax: 215-343-0214
E-mail: pmarcum-at-polysciences.com


My first question is, what proof was the tequila? If it was potent stuff,
the ethanol level may have been high enough to act as a coagulative
fixative. Many years ago I was asked to do EM of the gills of clams that
had been fixed in 70% ethanol. I rehydrated them to buffer and then
processed for EM as usual (glut, osmium, etc). The ultrastructure, while
clearly compromised was far better than I had expected. You can try this
with the ovary samples. The fact that they are destined for paraffin means
that you won't have the very fine structure to worry about. You might want
to use a higher than usual concentration of pfa, or even throw in a little
glut to lock in whatever is left. Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


For TEM I have looked at skin and underlying connective tissue from all
kinds of
strange scenarios, eg. collected from a dead emu under freezing weather
conditions for a number of days. Expect loss of fine detail and for certain
organelles to be much more poorly preserved than others. You might take some
samples and run them down to buffer then refix in buffered formaldehyde. Embed
some other samples straight from the tequila. I suspect the tequila is between
40-60% ethanol.
Don't waste the tequila, maybe you can start a fad, a pleasent way to increase
fertility, or some other patent medicine application. Bruce Cutler, Ph.D.
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas

Not too dumb on the part of the enviro's. After all, Tequila does the worm!
Tequila, as I quickly discovered, can have between 35-55% ethanol.
My recommendation would be to proceed with your own fixation and not worry.
They used their heads when they realized they went into the field
unprepared, or they had an accident, or they couldn't take the fixative with
them and couldn't find any there. You treat the tissue as if it just got to
you. The nitrogens are likely still waiting for the formaldehyde. Or, you
could transfer them to Clarke's 3:1(Absolute ethanol:Glacial Acetic Acid),
and have a really granular texture in the sections. Or Bouin's, which would
also complete the job on the worm.Regards,Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu


I do a lot of ovaries here - A LOT, but they are usually rodent and are
fixed in 10% formalin, Bouins or 70% ETOH not tequila (what a waste of good
tequila - assuming that it was of the good variety). I hope that the
ovaries were small or trimmed before they were plunked into the tequila.
I'd process them mostly out of curiosity to see how they might turn out.
The ultrastructure wouldn't be much to speak of but there might be some
histology to see. One reply on the microscopy listserver stated that
tequila is about 40% alcohol. If so I'd trim the samples if necessary and
process as I usually do by starting in 70% ETOH through 80%, 95% x2, 100%
x3, Xylene x 2, paraffin x 2.
The replier on microscopy also said to rehydrate in PBS and refix in
formalin. You could do that. In fact this might be a good idea it might
help to preserve what hasn't already been destroyed.
I hope Rosemary processes the wolf ovaries and gives a report on the
outcome.

Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
Sr. Research Specialist University of Arizona :
(office: AHSC 4212) P.O. Box 245044 :
(voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
(FAX: 520-626-2097) (email: algranth-at-u.arizona.edu) :

I think the active ingredient would be the ethanol content of tequila. No,
I am not laughing, when Lord Byron died somewhere in Mediterrean area or
whereever, his body was preserved in a wine or brandy cask and shipped home
(to England?) for burial.

She might want to know what the percentage of alcohol is, what proof, and
work on processing from that point. I would love to know the outcome of
this, I used Chinese vodka as a means to clean a scratch in skin while in
that country and prevent a skin infection, it worked but I had a quart of
the stuff, gave it to some person who loved the stuff in coca cola! Skin
healed well, his liver suffered badly!

As for humor, it could be said the tissues were well "pickled", same as
people are when they ingest too much of this stuff!!
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

It's possible, that the ovaries are fixed OK, provided they were cut open
when placed into the tequila. Normally, 70% ethanol would have been better,
maybe they should have used for alcohol fixation. If they have not been
cut open prior to fixation, then the odds are that the morphology of the
internal areas of the ovary will not be very good. However, try one sample,
trim to desired sized and post-fix in 10% formalin overnight, process for
paraffin embedding.
Karen Jensen, M.S.
Associate Scientist and Project Manager
University of Rochester Medical Center
Electron Microscope Research Core
Pathology and Lab. Med.
Rochester, NY 14642



From daemon Wed Nov 14 22:27:26 2001



From: Xianglin_Li-at-student.uml.edu (Xianglin Li)
Date: Wed, 14 Nov 2001 22:11:12 -0600
Subject: AFM/EFM to characterize the oxidation layer

Contents Retrieved from Microscopy Listserver Archives
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Hi, I have a question about the GaSb.

How to use AFM/EFM to characterize the oxidation layer which is above the
GaSb surface.

Thank you for your help!

Xianglin Li

University of Massachusetts
978-934-3411


From daemon Thu Nov 15 08:36:58 2001



From: Dave Hall :      daveh-at-restechimage.com
Date: Thu, 15 Nov 2001 09:25:18 -0500
Subject: RE: RE: Nikon camera-microscope interfacing

Contents Retrieved from Microscopy Listserver Archives
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The C-mount interface suggestions below should work and will result in
an image being available to your D1 sensor. However, if your solution
requires that you invest more money into the process, you might consider
additional alternatives geared more towards 35mm SLR photography to
optimize your results. With the D1 being an SLR camera body, and given
the pixel resolution of the sensor, for our D1 customers, we have,
instead, used one of two options which, we believe, result in optimal
sensor fill and end results.

1.) Diagnostic Instruments has a photographic adapter series for
coupling 35mm SLR cameras to microscopes. Within that series, the
PA1-12A with the NIKC-T2 adapter usually fits the bill for most Nikon
scopes. Diagnostic Instruments recommends a Nikon 1.6x photoeyepiece
for image projection.

2.) Alternatively, I believe Nikon now has a direct Nikon option for
the D1 series of cameras. It does pretty much the same thing as Option
1, providing the 1.6x projection, except it all comes from Nikon, and
might be slightly more "refined".

Diagnostic Instruments products are available through a network of
dealers, most of which are also microscope dealers. Nikon products are
(obviously) available from your local Nikon dealer. You might consult
your Nikon dealer in regard to either solution. They can probably offer
both.

PLEASE NOTE: This e-mail is in no way meant to pass judgment on the
suggestions of other list members. I only provide the above info based
on opinion and the approach we have taken in this situation.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045
*http://www.restechimage.com

-----Original Message-----
} From: "tartenon-at-netscape.net"-at-sparc5.microscopy.com
[mailto:"tartenon-at-netscape.net"-at-sparc5.microscopy.com]
Sent: Wednesday, November 14, 2001 4:08 PM
To: Microscopy-at-sparc5.microscopy.com


C-Mount is just an adapter and wont requiere any additional lens, You'll
get a direct image. You can use different c-mounts with zoom in them in
order to get a larger field of view. The lens you require will depend on
the chip size of your camera (DN100 has 1/2" chip size) so if you want a
larger field of view you probably want to buy a C mount + TV adapter
0.45X The DXM1200 has a 2/3" chip size therefore the best combination
will be C mount + TV adapter 0.6X

Alfredo
"michael shaffer" {rarewolf-at-roadrunner.nf.net} wrote:

} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From daemon Thu Nov 15 08:54:06 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 15 Nov 2001 09:49:10 -0500
Subject: Re: resins for culture dishes and plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Dear listers,
} Some time ago one of you discussed a combination of resins that
} snapped off culture dishes fairly easily. Could you post that again
} as I need the information and can't seem to locate it in the
} archives. I've tried several combinations of resins from different
} sources on different brands of plates and the results are not great.
} There also appear to be differences in the "snapability" between the
} multiple well plates and the individual dishes from the same
} companies, which adds to the problem. I'd really appreciate knowing
} the magic combination of resin components if you could readdress the
} issue.
} Thanks,
} Mary Gail Engle
}
****************
HI Mary Gail,

I was the one who posted my "magic mixture". It is this: LX112
(Ladd Industries), DDSA (Electron Microscopy Sciences), NMA (also
EMS) and DMP-30 (Ladd). I use a "standard" Epon recipe, nothing
secret there. After the last 100% ethanol, I pour a shallow layer of
resin into the dish and insert (tubes made by cutting the ends off
BEEM capsules) into the resin over areas of interest. Be sure that
the molded end of the tube is facing down so that you have a
perfectly flat edge. Polymerize overnight, then fill just the tubes
with more resin. Return to the oven for 24-36 hr. when you remove
then dish from the oven, just grasp the embedding tube with a pair of
needle-nosed pliers and snap. If anything, a small amount of the
dish may come off with your block, but it shoul.d not leave any cells
behind. I haven't seen a difference between single and multi-well
plates, although the small diameter wells often don't work well....I
think its tough to get a good exchange of the last alcohol and the
blocks tend to be gummy.

If you have any more questions, contact me off-list and will will elaborate.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Thu Nov 15 09:08:05 2001



From: Russell McConnell :      russell.mcconnell-at-tufts.edu
Date: Thu, 15 Nov 2001 10:02:48 -0500
Subject: Re: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers,
With the agave shortage a couple of years back, tequila is getting
expensive. What about gin? Or whiskey? Then we could give the
fixations cute names like 'The Limey' or 'Kentucky's Finest'
(respectively). The ethanol content would be about the same, and there
would be less impurities (unless those impurities are what makes it a
good fixative). Just a thought.

Gareth Morgan wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} No experience and can't think of any jokes but .....
}
} I guess tequila is about 40% ethanol or is this home-brewed stuff??
} Standardisation in the future could be a problem - will they be using the
} same brand next time?
}
} Limited lipid extraction in that case - wash in PBS and cryosection?
}
} You could rehydrate to PBS and refix in buffered formaldehyde - could help
} to protect against processing.
}
} I guess that wolf ovaries are resonably large so you could try various
} combinations?
}
} I would be interested to hear how it goes. Go for it and educate us all!
}
} At 19:46 2001-11-13 -0500, Rosemary Walsh wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} } I am expecting the following samples for paraffin embedding sometime
} } soon -- "wolf ovaries have been preserved in tequila for the past 6 months
} } or so". I first assumed that this was a joke but apparently they are
} } serious--this is a reproductive physiology project from an ecology
} } lab. Any comments are welcome--levity included. I can hear your laughter
} } already! My questions:
} } --has anyone used tequila as a fixative?
} } -- is the embedding worth pursuing?
} } Rosemary Walsh
} }
} }
}
} Med vänliga hälsningar/With best wishes
}
} Gareth
}
} "Close your eyes and look. What you saw at first is there no more; and what
} you will see next has not yet come to life" Leonardo da Vinci (1452-1519).
}
} http://www.ki.se/biomedlab
} e-mail Gareth.Morgan-at-impi.ki.se
}
} Tel +46 8 728 3734
} Fax +46 8 728 3688
}
} Gareth Morgan MPhil MSc FIBMS,
} Institutionen för Mikrobiologi,
} Patologi och Immunologi(IMPI), H5,
} Karolinska Institutet,
} Dept of Biomedical Laboratory Science,
} Lindhagensgatan 92, Box 12773,
} S 112 96, Stockholm
} Sweden
}
} OBS! Besöksadress: Lindhagensgatan 92
} NB! Visiting address:Lindhagensgatan 92

--
Russell McConnell
Confocal Imaging Facility Technician
Department of Neuroscience
Tufts University School of Medicine
M&V Building room #137
136 Harrison Ave.
Boston, MA 02111
Tel. (617) 636-3795


From daemon Thu Nov 15 12:50:11 2001



From: Sergei V. Kalinin :      sergei2-at-seas.upenn.edu
Date: Thu, 15 Nov 2001 13:40:14 -0500
Subject: Re: AFM/EFM to characterize the oxidation layer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
It all depends on which measurements you have in mind. In the simplest
case, you can use topographic imaging to trace the changes in topography
during oxidation. From these measurements, you can detect the formation of
oxide if it is accompanied by significant morphological changes, e.g. instead
of flat surface with atomic steps you see large (10-100 nm) oxide
crystallites. Of course, if oxide is uniform, you wouldn't see much.
If you want to get oxide thickness, you can etch it locally and measure
the step heigh between etched and unetched parts.
Electrostatic force microscopy and surface potential microscopies are
completely different story. In principle, on the potential map you can see
charged grain boundaries, trapped charges and other electroactive defects.
You can also measure CPD variations between different phases. Some useful
leads can be found in papers by J.W.P. Hsu, D.A. Bonnell et al and Ludeke and
Cartier, among others. However, interpretation of the SSPM images can be
tricky. The usual caveat is topographical artifacts, etc.
You might also consider asking the same question at DI forum at
spm-at-di.com
Hope this helps
Sergei

}
} Hi, I have a question about the GaSb.
}
} How to use AFM/EFM to characterize the oxidation layer which is above the
} GaSb surface.
}
} Thank you for your help!
}
} Xianglin Li
}
} University of Massachusetts
} 978-934-3411

--
Sergei V. Kalinin
Dept. Mat. Sci. Eng.
University of Pennsylvania,
3231 Walnut St, Philadelphia PA 19104
Tel: (215) 898-3446
Fax: (215) 573-2128
E-mail: sergei2-at-seas.upenn.edu
URL: http://www.seas.upenn.edu/~sergei2




From daemon Thu Nov 15 14:20:03 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 15 Nov 2001 14:18:12 -0600
Subject: Re: a sample prep queri

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bacardi 151 proof rum made a good fixative for marine organisms ...

Phil

} Hi listers,
} With the agave shortage a couple of years back, tequila is getting
} expensive. What about gin? Or whiskey? Then we could give the
} fixations cute names like 'The Limey' or 'Kentucky's Finest'
} (respectively). The ethanol content would be about the same, and there
} would be less impurities (unless those impurities are what makes it a
} good fixative). Just a thought.
}
} Gareth Morgan wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } No experience and can't think of any jokes but .....
} }
} } I guess tequila is about 40% ethanol or is this home-brewed stuff??
} } Standardisation in the future could be a problem - will they be using the
} } same brand next time?
} }
} } Limited lipid extraction in that case - wash in PBS and cryosection?
} }
} } You could rehydrate to PBS and refix in buffered formaldehyde - could help
} } to protect against processing.
} }
} } I guess that wolf ovaries are resonably large so you could try various
} } combinations?
} }
} } I would be interested to hear how it goes. Go for it and educate us all!
} }
} } At 19:46 2001-11-13 -0500, Rosemary Walsh wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Listers,
} } } I am expecting the following samples for paraffin embedding sometime
} } } soon -- "wolf ovaries have been preserved in tequila for the past 6 months
} } } or so". I first assumed that this was a joke but apparently they are
} } } serious--this is a reproductive physiology project from an ecology
} } } lab. Any comments are welcome--levity included. I can hear your laughter
} } } already! My questions:
} } } --has anyone used tequila as a fixative?
} } } -- is the embedding worth pursuing?
} } } Rosemary Walsh
} } }
} } }
} }
} } Med vänliga hälsningar/With best wishes
} }
} } Gareth
} }
} } "Close your eyes and look. What you saw at first is there no more; and what
} } you will see next has not yet come to life" Leonardo da Vinci (1452-1519).
} }
} } http://www.ki.se/biomedlab
} } e-mail Gareth.Morgan-at-impi.ki.se
} }
} } Tel +46 8 728 3734
} } Fax +46 8 728 3688
} }
} } Gareth Morgan MPhil MSc FIBMS,
} } Institutionen för Mikrobiologi,
} } Patologi och Immunologi(IMPI), H5,
} } Karolinska Institutet,
} } Dept of Biomedical Laboratory Science,
} } Lindhagensgatan 92, Box 12773,
} } S 112 96, Stockholm
} } Sweden
} }
} } OBS! Besöksadress: Lindhagensgatan 92
} } NB! Visiting address:Lindhagensgatan 92
}
} --
} Russell McConnell
} Confocal Imaging Facility Technician
} Department of Neuroscience
} Tufts University School of Medicine
} M&V Building room #137
} 136 Harrison Ave.
} Boston, MA 02111
} Tel. (617) 636-3795

--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Nov 15 14:20:03 2001



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Thu, 15 Nov 2001 12:15:00 -0800
Subject: Microscopy Technician - SF Bay Area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy technician vacancy in Albany, California (East San Francisco Bay Area), near Berkeley, California.


An individual is needed to provide assistance and expertise in scanning electron and light microscopy on various projects in food and non-food applied research. In addition, the person will provide expertise in photography, imaging, and preparation of graphics for presentation and publication using digital cameras and Windows-based computers. Applicants will be considered at the GS-5 through GS-7 levels.


Those interested, please see the Federal website for further information about the position and how to apply.


http://www.afm.ars.usda.gov/divisions/hrd/vacancy/D2W-2033.htm

{bold} ********************************************************************

Delilah F. Wood

Botanist

USDA-ARS-WRRC

800 Buchanan St.

Albany, CA 94710


Tel: 510-559-5653

Fax: 510-559-5818 {/bold}


From daemon Thu Nov 15 16:34:33 2001



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Thu, 15 Nov 2001 17:20:17 -0500
Subject: Looking for Barium Permanganate supplier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


O.k., this should NOT be this hard!

I am looking for a supplier for Barium permanganate (for use as a TEM
Stain), and I can't find anyone who carries it (Fisher, Sigma, EMS, Pella,
SPI, Ladd, etc.).

Any suggestions?

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."


From daemon Thu Nov 15 17:18:56 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 16 Nov 2001 10:22:09 +1100
Subject: Re: Powers of 10 Website

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have just seen an Imax film "Cosmic Journey" which expands on the plot of
"Powers of Ten" adds some graphics and presents a (speeded up!) depiction
of the early development of the Universe and all that. Its worth looking
out for.


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Fri Nov 16 05:15:56 2001



From: Jonathan Wilson :      wilson_jm-at-cimar.org
Date: Fri, 16 Nov 2001 10:59:47 -0800
Subject: microtome oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I have been loaned a microtome (Leitz 1512) by a colleague which
according to the instruction manual requires regular oiling. The microtome
did not come with any oil and the price I have been quoted by a local
supplier for microtome oil was about 75$US (including VAT) for 50ml (SAKURA
1447). I found the price a bit steep and am left wondering if it is
absolutely necessary to buy "microtome" oil or if I can use an off the shelf
oil (e.g 3 in 1 or something like that). I do not want to compromise the
performance of the microtome as it is not mine and would appreciate any
advice from those with experience.
Thanks for any help.
Sincerely,
Jonathan

Jonathan Wilson (PhD)
CIIMAR
Rua do Campo Alegre 823
4150-180 Porto, Portugal
tel: 351 22 608 0470 / 71
fax: 351 22 606 0423
e-mail: wilson_jm-at-cimar.org
web: www.cimar.org




From daemon Fri Nov 16 07:59:17 2001



From: Jonathan Wilson :      wilson_jm-at-cimar.org
Date: Fri, 16 Nov 2001 13:51:33 -0800
Subject: microtome oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I have been loaned a microtome (Leitz 1512) by a colleague which
according to the instruction manual requires regular oiling. The microtome
did not come with any oil and the price I have been quoted by a local
supplier for microtome oil was about 75$US (including VAT) for 50ml (SAKURA
1447). I found the price a bit steep and am left wondering if it is
absolutely necessary to buy "microtome" oil or if I can use an off the shelf
oil (e.g 3 in 1 or something like that). I do not want to compromise the
performance of the microtome as it is not mine and would appreciate any
advice from those with experience.
Thanks for any help.
Sincerely,
Jonathan

Jonathan Wilson (PhD)
CIIMAR
Rua do Campo Alegre 823
4150-180 Porto, Portugal
tel: 351 22 608 0470 / 71
fax: 351 22 606 0423
e-mail: wilson_jm-at-cimar.org
web: www.cimar.org



From daemon Fri Nov 16 11:34:11 2001



From: Haixin Xu :      xu-at-gl.umbc.edu
Date: Fri, 16 Nov 2001 12:23:08 -0500 (EST)
Subject: membrane preservation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

We are trying to microscopy a microorganism, spiroplasma using TEM. Every
time we had problem to see clearly the membrane around the organism. We
tried to increase the time of Osmium (4%) fixation, 3-5 hours at RT after
glutaldehyde fixation. But the membrane preservation is still terrible. I would very much appreciate
any of suggestions for that.

Haixin Xu
UMBC/BS

Baltimore, MD. 21250



From daemon Fri Nov 16 13:41:28 2001



From: mancini :      mancini-at-bcm.tmc.edu
Date: Fri, 16 Nov 2001 13:33:13 -0600
Subject: Microscopy Core Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Core Technician

The Integrated Microscopy Core, Department of Molecular and Cellular
Biology, Baylor College of Medicine has an immediate full-time opening for
an electron microscopy technician. The Integrated Microscopy Core is a busy,
state-of-the-art facility with a new Hitachi H7500 equipped with Gatan’s new
2Kx2K CCD camera, deconvolution, laser scanning confocal, and 2 CCD-based
upright and inverted epifluorescence microscopes.

The applicant should have at least one year of experience in various aspects
of sample preparation for biological TEM. This should include fixation,
embedding, ultrathin sectioning staining and darkroom procedures, TEM
operation and knowledge of computers. Other duties include preparation of
solutions, embedding media and excellent organizational skills.

The position offers opportunities for training in advanced microscopy
techniques, including digital TEM, fluorescence, deconvolution and laser
scanning confocal microscopy. Excellent communication skills and a
Bachelor's degree are required. A competitive salary and benefit package
commensurate with experience is offered. Please email a cover letter
expressing your research/technical interests and resume to:

Michael A. Mancini, Ph.D.
Assistant Professor
Director, Integrated Microscopy
Department of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX 77030
mancini-at-bcm.tmc.edu
http://microscopy.bcm.tmc.edu
713 798 8952



From daemon Fri Nov 16 13:48:19 2001



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Fri, 16 Nov 2001 14:43:13 -0500
Subject: Silver/carbon Paint composition when Dry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Y'All,
I'm interested to know what exactly is left behind after Ag (or C)
paint (used as an electrical contact on a sample stub) has dried. I have
used this method of attaching samples to holders in ultra high vacuum
systems and after the paint is thoroughly dried there is no outgassing in
uhv to 10-10 torr. However, I wondered if anyone has any idea what the
remaining constituents or composition of the paint would be after drying.

Thanks in advance,

Steve

Steve Buckingham
Dir. of Process Development
Excellatron Solid State LLC
1640 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 617 812 5920







From daemon Fri Nov 16 18:43:58 2001



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Fri, 16 Nov 2001 18:36:31 -0600
Subject: Luxol Fast Blue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Michelle, I have good results staining respiratory mucosal tissue embedded
in Araldite 502 with Alcian blue (8GS?) with or without PAS and in
conjugation with Weigert's hematoxlyn. The plastic is first etched 1 to 3
minutes with sodium ethoxide, one:one, with toluene. After that I followed
standard histological protocols. However, the staining times need to be
increased and/or heated to compensate for the reduced number of available
staining sites in semithins. The sections were spectacular I don't mind
saying.

Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030











Hello fellow microscopists-

Does anyone have any experience with staining thick (semi-thin) sections
with Luxol Fast Blue?

Any recommendations or thoughts are appreciated.

Thank you-
Michelle Taurino

Michelle Taurino
Aventis Pharmaceuticals
Senior Scientist
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com



From daemon Fri Nov 16 19:36:31 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Fri, 16 Nov 2001 17:30:04 -0800
Subject: membrane preservation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haixin,

I believe it's the nature of the Spiroplasma genus membrane structure rather
than the poor preservation from your side. The usual glut/OsO4 fixation should
be just fine, the problem is in visualization - lack of contrast. There is a
good site on http://www.oardc.ohio-state.edu/spiroplasma/what.htm explaining
evolutionary degeneration of the cell walls of the whole class Mollicutes. You
may want to try some other contrast enhancing methods in addition to traditional
heavy metal post-staining. Feel free to contact me off-line.

Alice Dohnalkova
Environmental Microbiology
Battelle, Pacific Northwest National Lab
Richland, WA 99352
(509)372-0692


-----Original Message-----
} From: Haixin Xu
To: Mel Dickson
Cc: microscopy-at-sparc5.microscopy.com
Sent: 11/16/2001 9:23 AM


Hi Listers,

We are trying to microscopy a microorganism, spiroplasma using TEM.
Every
time we had problem to see clearly the membrane around the organism. We
tried to increase the time of Osmium (4%) fixation, 3-5 hours at RT
after
glutaldehyde fixation. But the membrane preservation is still terrible.
I would very much appreciate
any of suggestions for that.

Haixin Xu
UMBC/BS

Baltimore, MD. 21250



From daemon Sat Nov 17 10:21:20 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 17 Nov 2001 11:04:29 -0500
Subject: Silver/carbon Paint composition when Dry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Steve Buckingham wrote:
==========================================================
} I'm interested to know what exactly is left behind after Ag (or C)
} paint (used as an electrical contact on a sample stub) has dried. I have
used
} this method of attaching samples to holders in ultra high vacuum systems
and
} after the paint is thoroughly dried there is no outgassing in uhv to 10-10
} torr. However, I wondered if anyone has any idea what the remaining
} constituents or composition of the paint would be after drying.
==========================================================
Our firm has had hands-on invovlement in the formulation of both silver and
carbon "paints" for SEM (including UHV) applications going back to the early
1970's. I think I can comment on this topic.

The silver and carbon systems have to be discussed separately.

In the case of the silver system, of the leading brands used in microscopy,
there is some considerable difference in the size and shape of the silver
colloid, the suspending organic carrier and the composition and loading of
polymer(s) that are present to impart to the paint different characteristics
. We have learned over the years that some polymer is important in order to
give the final paint layer the kind of adhesive properites users desire.
More can sometimes be "too much" and some polymers tend to be more effective
as an adhesive at low levels than other polymers. The shape of the colloid
(e.g. flat platelet vs. round sphere) is important as well, since some
sizes/shapes of the silver colloid lead to a higher likelihood of a skin
forming during drying, which then acts as a transport barrier so that there
is a wet region underneath. When such a skin-over-wet sample is inserted
into the vacuum system, we all know the consequences.

Finally, we have found that not all silver paints found in the microscopy
market have the same high purity, although we would be the first to
recognize that for most SEM users, the presence of such impurities (such as
contaminant SiO2 particles, otherwise known as glass frit) at low levels
would not make any difference. There is a difference in the % silver
loadings, something many just don't appreciate, and sometimes what appears
to be a "better price" deal in fact is a product that is "cheaper" because
of lower silver solids.......full disclosure on the percent silver solids at
least for the SPI Supplies products are given on our website. Further
evidence of the differences between products are the differences in flash
points as disclosed in MSDS information.

So to now answer the question, with regard to silver, after the liquid
carrier evaporates completely, with no wetness under a skin, there should be
only the silver colloid, polymer at a low level, and otheriwise unintended
impurities. Most people report that when the dried SPI Supplies silver
paint is inserted into a UHV sustem, they find no indication of a
diminuition of the quality of the vacuum.

For the carbon paint, the system is a bit easier to describe, the colloid is
present much as is the silver colloid, a bit of a polymer to give the carbon
paint greater adhesion properties, and a low level of impurities. Again,
the carbon paints are not "all the same". There are differences in the
carbon colloid size, difference in the polymer, and differences in the
impurity elements that might be found. When the carbon paint dries, there
is also a tendency for a skin to form, and since there are differences in
the formulations, there are differences in the tendency for skin formation.
But when fully dry, and there is no organic carrier underneath a skin, there
should be only the carbon colloid, the polymer, and unintended impurities
(which vary from product to product). We have had similar reports with
regard to the SPI Carbon Paint product, that when inserted into a UHV system
, there is no indication of a diminuiution of the quality of the vacuum.

Disclaimer: For nearly thirty years, SPI Supplies has formulated silver and
carbon paints for SEM/EDS and other vacuum applications, and we have been
able to do extensive testing on our own in-house equipment of various
formulations. Now despite our obvious eagerness to sell more, I would
urge consideration of the dry adhesives, mainly the double sided conductive
carbon sheets and the analogous double sided silver sheet products. They
too seem to be equally vacuum compatible and one eliminates the uncertainly
of any kind of "wet" region underneath a skin on the surface. One also
eliminates the possibility of inhalation of organic vapors. Such double
sided (dry) adhesives are available from SPI Supplies as well as from the
main supply sources for consumables for SEM and TEM. But like with the
silver and carbon paint products, they don't all "come from the same place"
and your experiences with one might not necessarily be the same as with the
others. You might want to try more than one kind and then draw your own
conclusions.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Sat Nov 17 18:32:48 2001



From: Erinasilk-at-aol.com ()
Date: Sat, 17 Nov 2001 18:19:40 -0600
Subject: Ask-A-Microscopist: what power microscope do I need to see

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Erinasilk-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
November 17, 2001 at 15:31:20
---------------------------------------------------------------------------

Email: Erinasilk-at-aol.com
Name: Erin Silk

Organization: Summit Middle Schoo

Education: 6-8th Grade Middle School

Location: Boulder,Colorado,USA

Question: What power microscope do I need to see bacteria grow on
samples of meat. I have two microscopes one that is up to 43x and
another at up to 900x. I want to see and measure if and how much
bacteria is present in my slide samples.

---------------------------------------------------------------------------


From daemon Sun Nov 18 01:20:14 2001



From: Marco Arienti :      marienti-at-tiscalinet.it
Date: Sun, 18 Nov 2001 08:09:30 +0100
Subject: Film to ccd camera converter

Contents Retrieved from Microscopy Listserver Archives
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Hi listers!

I am looking for a strange device, but I suppose already someone see
something like hat.

A friend of mine is an optician and got a slit lamp.

He is now picking up pictures to be stored in a computer with a TV camera.

Obviously, after the frame grabber, he get an ugly quality of the image.

The slit lamp is equipped with a camera attachment and he got a camera too,
but the procedure of taking a picture with a film, develop it, scan and
store it is too long.

I remember of a converter device that fit in any 35 mm camera with a CCD
sensor able to convert a normal film camera in a digital one.

Anyone know about it?

Thanks a lot in advance.



Marco Arienti








From daemon Sun Nov 18 06:46:18 2001



From: a6863-at-iobox.fi
Date: Sun, 18 Nov 2001 07:34:21 -0500
Subject: Our Last 3 Picks Are Up Over 400%

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Biomasse International, Inc. (OTCBB BIMS)

Immediate & Strong BUY Recommendation

Huge Analyst and Newletter Coverage for BIMS

BIMS will soon be profiled by some major analysts
and newsletters along with the release of significant
news regarding explosive sales for the Company. There
will be huge volume and a strong increase in price for
several days. The same groups that featured TIWI will
begin coverage on BIMS. TIWI exploded from $ .44 to
$2.41 in 4 days!! We know for certain that the same
groups are going to feature BIMS and even better returns
are expected.

We are very proud that we can share this information with
you so that you can make a profit out of it. It is highly
advisable to take a position in BIMS as soon as possible,
today before the market closes or tomorrow.

The stock could easily reach $5.00 in less than a month on
the strength of their upcoming contract announcements and
Strong Analyst Buy Recomendations.

When word gets out this stock will SOAR!



From daemon Sun Nov 18 10:33:06 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 18 Nov 2001 08:26:28 -0800
Subject: Re: Film to ccd camera converter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try:

http://www.siliconfilm.com/

gary g.


At 11:09 PM 11/17/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Nov 18 10:34:55 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 18 Nov 2001 08:28:48 -0800
Subject: Re: Ask-A-Microscopist: what power microscope do I need to see

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Name: Erin Silk
}
} Organization: Summit Middle School
}
} Education: 6-8th Grade Middle School
}
} Location: Boulder,Colorado,USA
}
} Question: What power microscope do I need to see bacteria grow on
} samples of meat. I have two microscopes one that is up to 43x and
} another at up to 900x. I want to see and measure if and how much
} bacteria is present in my slide samples.
}
Erin -

The "900x" may do the job for you. But since anything above 400x SHOULD
require what a microscopist calls an "oil immersion objective", I fear that
your lenses will not give you a clear image. The best way available to you
for actually seeing the bacteria will be to use a sterile swab to take a
sample to smear on a slide; you'll probably need to stain them to make them
visible. Getting accurate quantitative data probably won't be possible
with the methods available to you. Look in catalogs like Flinn Scientific,
Carolina Biological, or Edmund Scientific for kits with stains and
instructions. If you can't see them, consider trying to grow the bacteria
on culture medium in petrie dishes. You can find supplies in the same
catalogs.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Nov 18 11:23:36 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Sun, 18 Nov 2001 11:21:02 -0600
Subject: Re: Silver/carbon Paint composition when Dry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This depends on whose paint you use. Ted Pella, SPI, EMS, etc. all
use different solvents (such as methylethylketone, acetone, and
others), and they use some sort of polymer (acrylic?) as a binder.
The best thing is to get the MSD sheet for the particular brand you
use, and call the folks from whom you bought the paint.

Phi

} Hi Y'All,
} I'm interested to know what exactly is left behind after Ag (or C)
} paint (used as an electrical contact on a sample stub) has dried. I have
} used this method of attaching samples to holders in ultra high vacuum
} systems and after the paint is thoroughly dried there is no outgassing in
} uhv to 10-10 torr. However, I wondered if anyone has any idea what the
} remaining constituents or composition of the paint would be after drying.
}
} Thanks in advance,
}
} Steve
}
} Steve Buckingham
} Dir. of Process Development
} Excellatron Solid State LLC
} 1640 Roswell Street, Suite J
} Smyrna, GA 30080
} phone 770 438 2201
} fax 617 812 5920

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Sun Nov 18 12:13:03 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 18 Nov 2001 15:21:31 +1000
Subject: RE: microtome oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unless I'm dreadfully mistaken a microtome does not produce extreme heat (like
an engine) and would not be used at -100C. The oil is required for lubrication
and should cause little drag due to high viscosity. Sounds like sewing machine
oil to me. If it was my microtome, I'd use some Molyslip (molybdenum
disulfide), which lubricates better than oil, does not lose its properties,
sticks better then grease and does not increase drag like grease.
Until better reasons are provided, it appears somebody is selling snake-oil.
Many years ago a top engineer from Ford Motors compared fancy oil additives
with quality engine oil thus:
They are mouse milk and very precious, but don't do anything that Cows-milk
won't do.

On second thoughts Jonathan: I'll provide twice the volume and include freight
cost. Maybe we can corner the world market in "microtome oils".
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, November 17, 2001 7:52 AM, Jonathan Wilson
[SMTP:wilson_jm-at-cimar.org] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I have been loaned a microtome (Leitz 1512) by a colleague which
} according to the instruction manual requires regular oiling. The microtome
} did not come with any oil and the price I have been quoted by a local
} supplier for microtome oil was about 75$US (including VAT) for 50ml (SAKURA
} 1447). I found the price a bit steep and am left wondering if it is
} absolutely necessary to buy "microtome" oil or if I can use an off the shelf
} oil (e.g 3 in 1 or something like that). I do not want to compromise the
} performance of the microtome as it is not mine and would appreciate any
} advice from those with experience.
} Thanks for any help.
} Sincerely,
} Jonathan
}
} Jonathan Wilson (PhD)
} CIIMAR
} Rua do Campo Alegre 823
} 4150-180 Porto, Portugal
} tel: 351 22 608 0470 / 71
} fax: 351 22 606 0423
} e-mail: wilson_jm-at-cimar.org
} web: www.cimar.org



From daemon Mon Nov 19 07:42:11 2001



From: eli rothenberg :      elir-at-chem.ch.huji.ac.il
Date: Mon, 19 Nov 2001 15:29:04 +0100
Subject: Wide illumination in fluorescence microscopy.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I am trying to build fluorescence confocal microscope using a laser,a
dichroic mirror, an objective(numerical aperture=0.7;working distance =
6 mm; focal length = 2 mm; focus depth 0.6 micron)which focuses into a
cryostat,the emmited light is focused into the monochromator with a
mirror in, and then to the CCD.

However, I did not achieve wide illumination, I've checked the spot size
with the CCD but it had hardly changed even though i added a short focus
lens in the exciting beam path and a diffuser. I also tried to put a
longer focusing lens in the exciting beam path so that the focal point
will be inside the objective but the image didn't widened.

Does anyone has an idea how to solve the problem and what lens or optics
i should use in the path of the exciting laser so that i would get wide
illumination. and a wide image as well.

Thanx.

Eli



From daemon Mon Nov 19 08:26:28 2001



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Mon, 19 Nov 2001 14:18:32 +0000 (GMT)
Subject: Fwd: FW: New Virus - Important

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



hello everyone,
I very rarely send out a blanket message - please bear
with me, but you might like to consider this virus
warning.
best wishes, Jeremy

--- "Sanderson, Yvonne (REPP)"
{Yvonne.Sanderson-at-repp.co.uk} wrote: } From:
"Sanderson, Yvonne (REPP)"
} {Yvonne.Sanderson-at-repp.co.uk}
} To: "'jb_sanderson-at-yahoo.com'"
} {jb_sanderson-at-yahoo.com}
} Subject: FW: New Virus - Important
} Date: Mon, 19 Nov 2001 14:19:21 -0000

} Importance: High
}
} Subject: New Virus - Important
}
Someone is sending out a very cute screensaver of the
Budweiser Frogs.
} } } } } } } } } }
If you download it, you will lose everything! Your
hard
} drive will crash
} } } } } } } } } } and someone from the Internet will
} get your screen name and
} } } } } } password!
} } } } } } } DO
} } } } } } } } } } NOT DOWNLOAD IT UNDER ANY
} CIRCUMSTANCES!
} } } } } } } } } } It just went into circulation
} yesterday. Please distribute
} } this
} } } } } } } } } } message.This is a new, very
} malicious virus and not many
} } people
} } } } } } know
} } } } } } } } about
} } } } } } } } } } it. This information was announced
} yesterday morning from
} } } } } } Microsoft.
} } } } } } } } } } Please share it with everyone that
} might access the
} Internet.
} } }
} }
}
}
}
***************************************************************************************
} The information in this internet eMail is
} confidential and is intended
} solely for the addressee(s). Access, copying,
} dissemination or re-use
} of information in it by anyone else is unauthorised.
} Any views or opinions
} presented are solely those of the author and do not
} necessarily represent
} those of Reed Educational & Professional Publishing
} or any of its affiliates.
} If you are not the intended recipient please contact
}
} Reed Educational & Professional Publishing, Oxford,
} +44 1865 311366.
}
***************************************************************************************
}

__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page from News and Sport to Email and Music Charts
http://uk.my.yahoo.com


From daemon Mon Nov 19 08:42:17 2001



From: Ann-Fook Yang :      yanga-at-EM.AGR.CA
Date: Mon, 19 Nov 2001 09:39:01 -0500
Subject: Re: membrane preservation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haixin,

You may try using low molecular weight tannic acid after osmium fixation.

Reference: Nicolae Simionescu and Maia Simionescu 1976. Galloylglycoses of low molecular weight as mordant in electron microscopy. The Journal of Cell Biology. Vol 70, 608-621

Annfook Yang


} } } Haixin Xu {xu-at-gl.umbc.edu} 11/16/01 12:23PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Listers,

We are trying to microscopy a microorganism, spiroplasma using TEM. Every
time we had problem to see clearly the membrane around the organism. We
tried to increase the time of Osmium (4%) fixation, 3-5 hours at RT after
glutaldehyde fixation. But the membrane preservation is still terrible. I would very much appreciate
any of suggestions for that.

Haixin Xu
UMBC/BS

Baltimore, MD. 21250





From daemon Mon Nov 19 11:03:07 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 19 Nov 2001 10:51:02 -0600
Subject: MSA Undergraduate Research Scholarship Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscopy Society of America
Undergraduate Research Scholarship Program

With this year's call for applications the MSA Undergraduate
Research Scholarship Program begins its 14th year providing funding
for undergraduate research. To date over 71 projects covering a wide
range of topics in the physical and biological sciences have received
support through this program. Over the years nearly all the
scholarship recipients have maintained a strong interest in imaging
sciences and have gone on to graduate school, professional school,
teaching, or industry positions.
The program, which is funded by MSA and by matching funds from MSA
Sustaining Members, is able to support approximately 30% to 40% of
applicants. This past year the MSA Executive Council approved an
increased in the amount provided by MSA. This, plus matching funds,
will permit funding of additional scholarships for 2002. The maximal
award for the Undergraduate Scholarships is $3000 and helps to
provide student stipends, supply costs, and limited travel expenses
associated with the research. Additional support in the form of
instrument use time, equipment purchases, etc. is generally provided
by the student's supervisor and/or through the sponsoring
institution. Abstracts reporting the research results, are prepared
by scholarship awardees, and published in "Microscopy and
Microanalysis."
The program actively seeks external sources of matching funds in
order to maintain the favorable levels of support both in terms of
the number of projects supported and the level of support for each.
We are extremely grateful for the matching support provided by MSA
sustaining members and individuals. Their support over the years has
enabled the program to increase both the number of awards and the
maximum amount of each award.

The MSA Undergraduate Research Scholarship Program is currently
solicting applications from students interested in conducting a
research project which involves the use of any microscopy technique.
Students should be sponsored by a member of MSA. The maximal award
is $3000. The application deadline is Dec 31, 2001. Applications
can be obtained from the MSA Business Office,
businessoffice-at-msa.microscopy.com or call toll free at (800) 538-3672.
If you have any questions or require additional information regarding
the program please contact either:

Dr. Ralph Albrecht, University of Wisconsin
1675 Observatory Drive, Madison, WI 53706
(608) 263-3952 or 263-4162; (608) 262-5157 FAX;
albrecht-at-ahabs.wisc.edu

Dr. Richard Ornberg, Monsanto Company
Analytical Sciences Center, 800 N. Lindbergh Blvd., St. Louis, MO 63167
(314) 694-1184; (314) 694-6727 FAX;
rlornb-at-ccmail.monsanto.com


This Year's MSA Undergraduate Research Scholarship Awardees and
Alternates were:

Awardees:
JORGE GARCIA
"Frictional and Mechanical Interactions between Nanotubes and
Self-Assembled Monolayers: An Atomic Force Microscope Study"
University of Texas-El Paso/Univ. Wisconsin, Madison
Advisor: Robert Carpick

JAYAN RAMMOHAN
"Imaging Aggregation Proteins InVitro Using Atomic Force Microscopy"
Case Western Reserve University
Advisor: Steven Eppell

PETER KRSKO
"Electron Beam Lithography for Micro-Patterning of Bioactive Surfaces"
Stevens Institute of Technology
Advisor: Matthew Libera

DANIEL J. MAZEAU
"Three-Dimensional Structure of the Human TFIIH-IIE Complex"
University of California-Berkeley
Advisor: Eva Nogales

DANIEL L. PECHKIS
"The Impact of Processing on the Interface between Thin Film
Dielectrics and their Silicon Substrates"
Southern Connecticut State University
Advisor: Christine Broadbridge

EUGENE KUNG
"HR-TEM Studies of Polymer-Carbon Nanotube Composites"
Princeton University
Advisor: Nan Yao

Alternates
RENEE LOPEZ SMITH
"Comparisons Between Pre-Released and Swimming Sperm Cells of the
Fern Lygodium: A Correlated SEM, TEM, and Light Microscope Study"
Southern Illinois University
Advisor: Karen Renzaglia

ERIN GRUND
"Quantitation of Glomerular Cell Number in Diabetic Patients and
Normal Controls"
University of Minnesota
Advisor: John Basgen

--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Mon Nov 19 13:47:06 2001



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 20 Nov 2001 08:36:53 GMT+1200
Subject: Anyone from Nikon listening?

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who replied to my posting last week about
camera-to-microscope interfacing, and for the suggestions, many of
which suggested contacting my local Nikon rep.

I would be perfectly happy to use Nikon parts, after all, I'm trying
to arrange a marriage between a Nikon camera and a Nikon microscope,
so it shouldn't be THAT difficult.

Unfortunately, in my country there is no direct Nikon presence, only
local representatives, but one company handles cameras, another
handles microscopes, so the question of their interfacing kind of
falls into a small but deep void.

If any expert from Nikon is reading this list, I would be grateful if
they would reply to me directly, so that we could engage in a
constructive dialogue.

thanx

rtch



}
} Hi
}
} We have a Nikon Labophot-POL microscope.
}
} In its trinocular head there sits a CF PL 5X Projection lens, above
} that is a Microflex HFX-IIA Photomicrographic Attachment, and onto
} that is a "35mm camera adapter A", then what is called in the
} HFX-IIA manual a "Motorized dark box FX-35WA". The latter looks more
} like a camera body to me.
}
} We want to be able to mount our Nikon D 1 body onto the microscope,
} with or without the HFX-IIA, whatever it takes.
}
} Is there anyone out there who can tell, from the above, what I need
} to do to acheive this?
}
} I would have thought that all we need to do would be to mount the D
} 1 body directly onto the trinocular vertical tube, but then I know
} almost nothing about light microscopy.
}

Dr Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand


From daemon Mon Nov 19 13:47:06 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 19 Nov 2001 11:38:05 -0800
Subject: Invention & Technology

Contents Retrieved from Microscopy Listserver Archives
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Hi:

Just received the Winter 2002 issue of American Heritage of Invention and
Technology, a quarterly magazine with some great articles.

This issue, Vol.17, #3, has an article on the 'birth of the electron
microscope'.

Palucka, Tim. Making the Invisible Visible. 2001, American Heritage of
Invention and Technology, American Heritage, New York, 17(3):12 - 23.

Haven't read it all yet, looks nice.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Nov 19 13:47:09 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 19 Nov 2001 14:39:51 -0500
Subject: cleaning printer rolls -See Nov MT p28

Contents Retrieved from Microscopy Listserver Archives
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I just read Gib Ahistrand's short in "Microscopy Today" about cleaning rolls in a laser printer. When I have problems with rubber rolls, either ink jet printers or laser printers, I rub the rubber with a lintless cloth moistened with isopropanol. It removes the "shine" that Gib is talking about. It definitely fixes the problem on inkjet printers where the paper will not feed from the stack.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)




From daemon Mon Nov 19 13:58:20 2001



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 19 Nov 2001 14:52:31 -0500
Subject: Re: membrane preservation

Contents Retrieved from Microscopy Listserver Archives
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Maybe try fast freezing plus freeze substitution? Either high pressure
freezing or slam freezing? This might better capture the membrane in its
native state. Then a freeze sub into osmium tetroxide in acetone should
give good contrast to the membrane.

David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
website: www.aecom.yu.edu/wormem


From daemon Mon Nov 19 14:26:02 2001



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Mon, 19 Nov 2001 14:18:06 -0600
Subject: Leica Confocal-Users' Forum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Confocal Microscopists:

I am organizing a Leica confocal user/administrator listserver in order to
facilitate and encourage dialogue regarding the problems, idiosyncrasies
and use of the Leica SP platform for confocal and multiphoton microscopy. I
am hoping that such a forum would be regarded as a logical extension of the
existing microscopy listserver for Leica confocal users to voice
Leica-specific questions/concerns.

A Leica LCSM/MP Listserver will allow us to archive questions and answers,
and may offset some of the perceived lack of documentation regarding the
software. As I have witnessed recently on the general confocal listserver,
Leica is paying attention to users' sharing their dilemmas, concerns and
successes. It would be advantageous for system administrators, end users
and Leica representatives to continue to build momentum in this direction.

LeicaSP_LCS_MP_Users is a subscriber access list-server devoted to
questions and issues unique and/or specific to the Leica SP Series Spectral
Confocal and Multiphoton System.

If you would like to subscribe, please send an e-mail message to:

listserv-at-listserv.uiuc.edu

with the body of the message consisting of:

subscribe LEICASP_LCS_MP_USERS {full name}

I will be delighted to manage/maintain a Leica confocal/mp listserver
resource. Let me know if you think if you have any thoughts, suggestions or
concerns regarding this user forum at your convenience. Thank you.

Best Regards,
Karl G.



_______________________________________________
Karl Garsha
Specialist in Light Microscopy
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Mon Nov 19 15:59:09 2001



From: Andre Blanchard :      andre.blanchard-at-venturechemicals.com
Date: Mon, 19 Nov 2001 15:52:32 -0600
Subject: Request for SEM and TEM services

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear members of the list:

I work for a company in Lafayette, Louisiana and we have an interest in
obtaining some SEM and TEM images of chitin isolated from crab shell waste.
Particularly, I am interested if anyone knows of institutions that provide
such a service, be it private or public, the cost of such services and
contact information. The chitin samples we have are in a flake form about 5
- 7mm square. None of the samples are ready for imaging, so they would need
to be prepared for the respective imaging processes. We would prefer the
images to be in a digital format.



Thank you in advance for any information received.

André N. Blanchard, Ph.D.
Manager-Technical and Business Development
The Venture Group, Inc
PO Box 53631
Lafayette, LA 70505-3631
U.S.A.

ph: (337) 232-1977
fx: (337) 237-5340

email: andre-at-vgweb.com
internet: http://www.vgweb.com




From daemon Mon Nov 19 15:59:10 2001



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Mon, 19 Nov 2001 16:52:20 -0500
Subject: Re: Leica Confocal-Users' Forum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A Leica listserv already exists and the reasons for the establishment of
that list several years ago were similar to those being touched upon here.
Traffic on that list has been essentially nil for the past two years. This
tells me one of three things: a) that nobody has any Leica-specific
problems that they feel can be solved by list interaction, b) that the
interaction received through the list was too limited to make the list a
useful resource, or c) that the list consists of "power users" who fix
their own problems. I suspect the answer is a mix of a and c. Certainly a
lot of Leica systems have been installed since the list was first put
together, and I am the first to admit that it was never well publicized,
partly for reasons that may become apparent below.

If you desire to move forward with establishment of this list (and I am not
discouraging you from doing so), then I would ask you to consider a few
points regarding how listservs work and who stands to benefit from the list.
1) is the list set up as "reply to all" or "reply to sender"?
2) can anyone subscribe (it would appear so) and post, or can only those
people with legitimate interests in Leica systems subscribe?
3) will Leica monitor the list? will they respond to posts to the list?
These two items are important. With the earlier (still existing) list, the
answers are "yes" and "no". I hope you can achieve a "yes" for both
questions, but you must at least achieve a "yes" for the first question,
otherwise it can be a needlessly unpleasant experience for the company
itself - very much a blindside tackle. If people simply want to talk about
how terrible tech support or service can be, then the list is pretty
useless (witness current BioRad postings) - everyone knows this is a sore
point with virtually every fancy piece of equipment sold from microscopes
to FACS machines. We don't need "venting" lists, particularly if the
people who should be "vented upon" are not listening (if they were, we
wouldn't be venting now would we??).

We are about to have a new SP2-MP-UV system installed here. My prediction
is that the learning curve on this system will be no steeper than that on
the TCS-NT system - fairly shallow but with a few specific headaches
(really just finding out which buttons to push when). What would really be
useful is a list for people who still have older instruments (e.g., TCS4D)
on which the learning curve is not only very steep but also for which the
repository of that knowledge is a handful of people worldwide. The final
point I would like to bring up is that the hardware/software configurations
of the various Leica machines sold over the years (even just SP/SP2 series)
can be very different, so it can be dangerous to generalize.

Rob Palmer
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396


From daemon Mon Nov 19 16:27:03 2001



From: Greg Strout :      stro8031-at-msmailhub.oulan.ou.edu
Date: Mon, 19 Nov 2001 16:16:40 -0600
Subject: Exhaust line for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
We have been using our Gatan duo mill for some time now with a copper
exhaust line on the rotary pump. It has worked fine, but now we have
someone who would like to use the iodine guns and I am wondering if we
should use a copper exhaust line with a corrosive gas like iodine. Does
anyone know if copper is an appropriate material to use for an exhaust
line when milling with iodine? Any additional pointers for milling with
iodine? TIA.
Greg

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Mon Nov 19 16:33:03 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 19 Nov 2001 17:37:32 -0500
Subject: RE: Gun valve operation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is difficult to answer the question Maxine Daws raised about the
operation of the gun and comumn valves in her electron microscope
without having a diagram of the vacuum system; however, operating
procedures for a variety of different vacuum systems (with a variety
of different kinds of pumps) are described in some detail in Chapter
9 of my book 'Vacuum Methods in Electron Microscopy' (see
http://www.2spi.com/catalog/books/book48.html and
http://pup.princeton.edu/titles/6484.html, for a description). If
you were to read this chapter, I believe that you could figure out
what the proper operating sequence might be. Otherwise, Maxine, if
you will send me a diagram of your system I will see if I can answer
your question.

Good luck & Happy Thanksgiving,
W. C. Bigelow
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Mon Nov 19 16:56:42 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 19 Nov 2001 16:49:58 -0600
Subject: Re: Silver/carbon Paint composition when Dry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



For the heck of it I did a quick & dirty EDS (thin window detector) on some old
carbon and Ag paint spots, no idea of who distributed this paint. The carbon had
significant peaks at C, O & P. The Ag had significant peaks at Ag, C & S. The
only surprise to me was the P peak with the carbon paint. The S on the Ag spot
probably came from Ag tarnishing in air.
For what its worth.....
Bruce
Bruce Cutler, Ph.D.
Director, Microscopy & Electronic Imaging Lab
University of Kansas


} } Hi Y'All,
} } I'm interested to know what exactly is left behind after Ag (or C)
} } paint (used as an electrical contact on a sample stub) has dried. I have
} } used this method of attaching samples to holders in ultra high vacuum
} } systems and after the paint is thoroughly dried there is no outgassing in
} } uhv to 10-10 torr. However, I wondered if anyone has any idea what the
} } remaining constituents or composition of the paint would be after drying.
} }
} } Thanks in advance,
} }
} } Steve
} }
} } Steve Buckingham
} } Dir. of Process Development
} } Excellatron Solid State LLC
} } 1640 Roswell Street, Suite J
} } Smyrna, GA 30080
} } phone 770 438 2201
} } fax 617 812 5920





From daemon Mon Nov 19 17:00:53 2001



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Mon, 19 Nov 2001 14:58:48 -0800
Subject: Re: Fwd: FW: New Virus - Important

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by sparc5.microscopy.com (8.9.3+Sun/8.9.3) id QAA18046
for dist-Microscopy; Mon, 19 Nov 2001 16:59:09 -0600 (CST)
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by SpamWall.lbl.gov (8.11.2/8.11.2) with ESMTP id fAJMsq526332;
Mon, 19 Nov 2001 14:54:52 -0800 (PST)
Message-ID: {3BF98EA8.C7832787-at-lbl.gov}


I believe that this is a hoax.
See:
http://www.f-secure.com/hoaxes/budfrogs.shtml
-Mike

Jeremy Sanderson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hello everyone,
} I very rarely send out a blanket message - please bear
} with me, but you might like to consider this virus
} warning.
} best wishes, Jeremy
}
} --- "Sanderson, Yvonne (REPP)"
} {Yvonne.Sanderson-at-repp.co.uk} wrote: } From:
} "Sanderson, Yvonne (REPP)"
} } {Yvonne.Sanderson-at-repp.co.uk}
} } To: "'jb_sanderson-at-yahoo.com'"
} } {jb_sanderson-at-yahoo.com}
} } Subject: FW: New Virus - Important
} } Date: Mon, 19 Nov 2001 14:19:21 -0000
}
} } Importance: High
} }
} } Subject: New Virus - Important
} }
} Someone is sending out a very cute screensaver of the
} Budweiser Frogs.
} } } } } } } } } } }
} If you download it, you will lose everything! Your
} hard
} } drive will crash
} } } } } } } } } } } and someone from the Internet will
} } get your screen name and
} } } } } } } password!
} } } } } } } } DO
} } } } } } } } } } } NOT DOWNLOAD IT UNDER ANY
} } CIRCUMSTANCES!
} } } } } } } } } } } It just went into circulation
} } yesterday. Please distribute
} } } this
} } } } } } } } } } } message.This is a new, very
} } malicious virus and not many
} } } people
} } } } } } } know
} } } } } } } } } about
} } } } } } } } } } } it. This information was announced
} } yesterday morning from
} } } } } } } Microsoft.
} } } } } } } } } } } Please share it with everyone that
} } might access the
} } Internet.
} } } }
} } }
} }
} }
} }
} ***************************************************************************************
} } The information in this internet eMail is
} } confidential and is intended
} } solely for the addressee(s). Access, copying,
} } dissemination or re-use
} } of information in it by anyone else is unauthorised.
} } Any views or opinions
} } presented are solely those of the author and do not
} } necessarily represent
} } those of Reed Educational & Professional Publishing
} } or any of its affiliates.
} } If you are not the intended recipient please contact
} }
} } Reed Educational & Professional Publishing, Oxford,
} } +44 1865 311366.
} }
} ***************************************************************************************
} }
}
} __________________________________________________
} Do You Yahoo!?
} Everything you'll ever need on one web page from News and Sport to Email and Music Charts
} http://uk.my.yahoo.com



From daemon Tue Nov 20 04:43:13 2001



From: Keith Ryan :      kpr-at-mba.ac.uk
Date: Tue, 20 Nov 2001 10:34:46 +0000
Subject: Optical bench available - in UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



An optical bench plus attachments is available in the Plymouth area, UK, following the passing-on of its owner.

If anyone is interested it can be removed, from an attic, without charge, but with not inconsiderable effort!

I can get more details if anyone is interested - it was not my field.

Keith Ryan
Marine Biological Association
Plymouth, UK





From daemon Tue Nov 20 08:54:15 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 20 Nov 2001 09:28:01 -0500
Subject: Exhaust line for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You need an activated carbon trap between your mechanical pump and your turbo. When I was at the University of Alabama at Birmingham, students on two occasions left the iodine gun valves open. They destroyed the mechanical pump springs and rendered it useless. I found out that we were not the only ones that this happened to. Gatan fixed a bunch of mechanical pumps under warranty and came up with a fix -a carbon trap with a sight glass on the mechanical pump side. (Our second pump failure was not covered.) Inside the tube where you could see, there was a silver strip that would change to black when the trap got saturated. You regenerated the trap by replacing the charge because otherwise you would be getting iodine into the mechanical pump. I would worry that copper might react similarly to iodine as the silver. The iodine does come out the exhaust of the mechanical pump.

I thought that the fix was rather expensive at the time, but it did work. You could probably get a zeolite trap and replace the zeolite with activated carbon from a pet store that sells aquarium supplies. Ten years ago, Gatan wanted $90 for a charge of activated carbon (about a quart jar). You could get twice that amount at a pet store for less than $10.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)

"The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."



-----Original Message-----
} From: Greg Strout [mailto:stro8031-at-msmailhub.oulan.ou.edu]
Sent: Monday, November 19, 2001 5:17 PM
To: Microscopy-at-sparc5.microscopy.com


Hi all,
We have been using our Gatan duo mill for some time now with a copper
exhaust line on the rotary pump. It has worked fine, but now we have
someone who would like to use the iodine guns and I am wondering if we
should use a copper exhaust line with a corrosive gas like iodine. Does
anyone know if copper is an appropriate material to use for an exhaust
line when milling with iodine? Any additional pointers for milling with
iodine? TIA.
Greg

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Tue Nov 20 09:25:32 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 20 Nov 2001 09:20:04 -0600
Subject: RE: Silver/carbon Paint composition when Dry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think you need to check your supply of paints.
I never had anything behind C and O on carbon paint,
do not remember S on Ag either.

Vladimir

}
} For the heck of it I did a quick & dirty EDS (thin window
} detector) on some old
} carbon and Ag paint spots, no idea of who distributed this
} paint. The carbon had
} significant peaks at C, O & P. The Ag had significant peaks
} at Ag, C & S. The
} only surprise to me was the P peak with the carbon paint. The
} S on the Ag spot
} probably came from Ag tarnishing in air.
} For what its worth.....
} Bruce
} Bruce Cutler, Ph.D.
} Director, Microscopy & Electronic Imaging Lab
} University of Kansas
}
}
} } } Hi Y'All,
} } } I'm interested to know what exactly is left behind
} after Ag (or C)
} } } paint (used as an electrical contact on a sample stub) has
} dried. I have
} } } used this method of attaching samples to holders in ultra
} high vacuum
} } } systems and after the paint is thoroughly dried there is
} no outgassing in
} } } uhv to 10-10 torr. However, I wondered if anyone has any
} idea what the
} } } remaining constituents or composition of the paint would
} be after drying.
} } }
} } } Thanks in advance,
} } }
} } } Steve
} } }
} } } Steve Buckingham
} } } Dir. of Process Development
} } } Excellatron Solid State LLC
} } } 1640 Roswell Street, Suite J
} } } Smyrna, GA 30080
} } } phone 770 438 2201
} } } fax 617 812 5920
}
}
}
}
}
}


From daemon Tue Nov 20 09:38:37 2001



From: gary.m.brown-at-exxonmobil.com
Date: Tue, 20 Nov 2001 09:33:28 -0600
Subject: Re: Fwd: FW: New Virus - Important

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Michael,

I suggest that you try Structural Research Inc. in Mobil, Alabama, (205)
649-9740. They come recommended although I have no personal experience with
them.

Best regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Polymers Center
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: (281) 834-2387
fax: (281) 834-2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



"Michael
O'Keefe" To: listserver a
{MAOKeefe-at-lbl.g {microscopy-at-sparc5.microscopy.com}
ov} cc:
Subject: Re: Fwd: FW: New Virus - Important

11/19/01 04:58
PM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I believe that this is a hoax.
See:
http://www.f-secure.com/hoaxes/budfrogs.shtml
-Mike

Jeremy Sanderson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hello everyone,
} I very rarely send out a blanket message - please bear
} with me, but you might like to consider this virus
} warning.
} best wishes, Jeremy
}
} --- "Sanderson, Yvonne (REPP)"
} {Yvonne.Sanderson-at-repp.co.uk} wrote: } From:
} "Sanderson, Yvonne (REPP)"
} } {Yvonne.Sanderson-at-repp.co.uk}
} } To: "'jb_sanderson-at-yahoo.com'"
} } {jb_sanderson-at-yahoo.com}
} } Subject: FW: New Virus - Important
} } Date: Mon, 19 Nov 2001 14:19:21 -0000
}
} } Importance: High
} }
} } Subject: New Virus - Important
} }
} Someone is sending out a very cute screensaver of the
} Budweiser Frogs.
} } } } } } } } } } }
} If you download it, you will lose everything! Your
} hard
} } drive will crash
} } } } } } } } } } } and someone from the Internet will
} } get your screen name and
} } } } } } } password!
} } } } } } } } DO
} } } } } } } } } } } NOT DOWNLOAD IT UNDER ANY
} } CIRCUMSTANCES!
} } } } } } } } } } } It just went into circulation
} } yesterday. Please distribute
} } } this
} } } } } } } } } } } message.This is a new, very
} } malicious virus and not many
} } } people
} } } } } } } know
} } } } } } } } } about
} } } } } } } } } } } it. This information was announced
} } yesterday morning from
} } } } } } } Microsoft.
} } } } } } } } } } } Please share it with everyone that
} } might access the
} } Internet.
} } } }
} } }
} }
} }
} }
}
***************************************************************************************

} } The information in this internet eMail is
} } confidential and is intended
} } solely for the addressee(s). Access, copying,
} } dissemination or re-use
} } of information in it by anyone else is unauthorised.
} } Any views or opinions
} } presented are solely those of the author and do not
} } necessarily represent
} } those of Reed Educational & Professional Publishing
} } or any of its affiliates.
} } If you are not the intended recipient please contact
} }
} } Reed Educational & Professional Publishing, Oxford,
} } +44 1865 311366.
} }
}
***************************************************************************************

} }
}
} __________________________________________________
} Do You Yahoo!?
} Everything you'll ever need on one web page from News and Sport to Email
and Music Charts
} http://uk.my.yahoo.com








From daemon Tue Nov 20 10:20:40 2001



From: Bob Roberts :      bobrobs-at-earthlink.net
Date: Tue, 20 Nov 2001 09:19:16 -0700
Subject: Re: Exhaust line for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Isn't it true some roughing pumps that are intended for this
type of application are teflon coated internally for a
barrier from corrosive gases?

Scott: By the way, the springs in that pump could have been
replaced...

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282



Walck, Scott D. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} You need an activated carbon trap between your mechanical pump and your turbo. When I was at the University of Alabama at Birmingham, students on two occasions left the iodine gun valves open. They destroyed the mechanical pump springs and rendered it useless. I found out that we were not the only ones that this happened to. Gatan fixed a bunch of mechanical pumps under warranty and came up with a fix -a carbon trap with a sight glass on the mechanical pump side. (Our second pump failure was not covered.) Inside the tube where you could see, there was a silver strip that would change to black when the trap got saturated. You regenerated the trap by replacing the charge because otherwise you would be getting iodine into the mechanical pump. I would worry that copper might react similarly to iodine as the silver. The iodine does come out the exhaust of the mechanical pump.
}
} I thought that the fix was rather expensive at the time, but it did work. You could probably get a zeolite trap and replace the zeolite with activated carbon from a pet store that sells aquarium supplies. Ten years ago, Gatan wanted $90 for a charge of activated carbon (about a quart jar). You could get twice that amount at a pet store for less than $10.
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} P. O. Box 11472 (letters)
} Guys Run Rd. (packages)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8515 (fax)
}
} "The opinions expressed are those of Scott D. Walck and not of PPG Industries, Inc. nor of any PPG-associated companies."
}
}
}
} -----Original Message-----
}
} } From: Greg Strout [mailto:stro8031-at-msmailhub.oulan.ou.edu]
} }
} Sent: Monday, November 19, 2001 5:17 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Exhaust line for ion milling
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hi all,
} We have been using our Gatan duo mill for some time now with a copper
} exhaust line on the rotary pump. It has worked fine, but now we have
} someone who would like to use the iodine guns and I am wondering if we
} should use a copper exhaust line with a corrosive gas like iodine. Does
} anyone know if copper is an appropriate material to use for an exhaust
} line when milling with iodine? Any additional pointers for milling with
} iodine? TIA.
} Greg
}
} --
} ==================================================================
} Greg Strout
} Electron Microscopist, University of Oklahoma
} WWW Virtual Library for Microscopy:
} http://www.ou.edu/research/electron/www-vl/
} e-mail: gstrout-at-ou.edu
} Opinions expressed herein are mine and not necessarily those of
} the University of Oklahoma
} ==================================================================
}
}
}
}
}




From daemon Tue Nov 20 10:48:41 2001



From: Smartech :      smartech-at-optonline.net
Date: Tue, 20 Nov 2001 11:50:38 -0500
Subject: Interesting interpretation of a SEM photomicrograph involving an

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I was analyzing a situation where some electroless (Cu bath that plates w/o
electricity) copper plate was peeling away from a copper substrate.

I have to clues. Oxidation and small blisters.

When I do a tape test I get a continuous sheet of electroless Cu on the back
of the tape and also a fresh surface of the Cu substrate. The first clue is
that with the naked eye you can see oxidation on both surfaces. To me this
means there must have been trapped Oxidation during the plating process.
Using SEM/EDS at low and medium kV, I did not observe any contamination on
either surface, just very thin oxidation.

The next clue was that when I took a photomicrograph with the polarity of
the Everhart and Thornley detector inverted I see a two types of
topographies. I see the expected micro-etch of the Cu substrate, but also
smooth bumps at 5-10 microns, as if the electroless plated some droplets. I
think these droplets are really blisters that occurred at very beginning of
the plating process. I feel that the blister cause the poor adhesion. I am
not sure what caused the blisters, maybe internal stress in the plated
deposit together with oxidized or very thin organic contamination of the
substrate. Or possibly gas evolution. The substrate has a very even etch
topography across the surface, no bumps. I would gladly e-mail these images
to anyone interested in seeing this unexpected use of a replicated surface.
I would also appreciate any comments.

Ric

SMARTech
860-491-3299
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Tue Nov 20 11:34:53 2001



From: sghoshro-at-NMSU.Edu
Date: Tue, 20 Nov 2001 10:18:28 -0700 (MST)
Subject: Nikon digital camera

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Hello Everyone,

We are looking into buying a Nikon coolpix 995 digital camera to be used
to take mostly brightfield images in a Zeiss Axioplan microscope. So I
would like to know how good this camera is for photomicrography. I also
found out Nikon has a new coolpix 5000 in the market. Does anyone have
good experience with this new one for our kind of application ?

Thanks a lot,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Director, Electron Microscopy Lab
Graduate Faculty, Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
URL:http://confocal.nmsu.edu/eml



From daemon Tue Nov 20 11:37:22 2001



From: Grizzi Fabio ICH :      fabio.grizzi-at-humanitas.it
Date: Tue, 20 Nov 2001 18:41:35 +0100
Subject: Basic histochemistry

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Dear All

I would like to receive any information about review papers on the basic
principles of histochemical techniques. I am quite interested to receive
references of papers on the basic characteristics of a histochemical method
(reproducibility, specificity etc).

Many thanks.

Fabio Grizzi
Scientific Direction
Istituto Clinico HUMANITAS
Milan, Italy


From daemon Tue Nov 20 11:45:23 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Tue, 20 Nov 2001 12:23:33 -0500
Subject: Tina Weatherby Carvaiho

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Hello, all,

I would like to get in touch with Tina to ask her a question. I cannot seem to find her email address. Can anyone help?

Better yet, Tina, if you're listening, could you please reply?

Thanks.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Tue Nov 20 14:30:52 2001



From: Imre Kovacs :      ikovacs-at-helix.mgh.harvard.edu
Date: Tue, 20 Nov 2001 14:57:37 -0500 (EST)
Subject: Re: Fwd: FW: New Virus - Important

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Dear microscopists,

There is no such thing...
Recently we have more and more spams and hoaxes, unfortunately. This virus
warning (and the others) is a big fat hoax
(http://www.symantec.com/avcenter/venc/data/buddylst.zip.html), only just
for clogging the net, generating chain-mails...
If you receive any "Important-Virus warning" in the future, please check
the Symantec page for hoaxes first
(http://www.symantec.com/avcenter/hoax.html).
Don't forward these messages automatically, especially not for lists or
discussion forums...
Regards,

Imre

Imre Kovacs M.D., Ph.D.
Research Fellow
Genetics and Aging Unit
Harvard Medical School
Massachusetts General Hospital East
Building 114, 16th Street
Charlestown, MA, 02129-4404
Phone: 617-724-3253
Fax: 617-724-1823

On Tue, 20 Nov 2001 gary.m.brown-at-exxonmobil.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Michael,
}
} I suggest that you try Structural Research Inc. in Mobil, Alabama, (205)
} 649-9740. They come recommended although I have no personal experience with
} them.
}
} Best regards,
}
} Gary M. Brown
} ExxonMobil Chemical Company
} Baytown Polymers Center
} 5200 Bayway Drive
} Baytown, Texas 77520-2101
} phone: (281) 834-2387
} fax: (281) 834-2395
} e-mail: Gary.M.Brown-at-ExxonMobil.com



From daemon Tue Nov 20 15:47:40 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 20 Nov 2001 16:41:48 -0500
Subject: Re: Basic histochemistry

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"Histochemistry" by Richard Horobin.

"Histological and Histochemical Methods" by John Kiernan

Most (all?) editions of "Cell and Tissue Biology" by Leon Weiss (editor) have a
nice chapter on histochemistry by Helen Padykula.

Grizzi Fabio ICH wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} I would like to receive any information about review papers on the basic
} principles of histochemical techniques. I am quite interested to receive
} references of papers on the basic characteristics of a histochemical method
} (reproducibility, specificity etc).
}
} Many thanks.
}
} Fabio Grizzi
} Scientific Direction
} Istituto Clinico HUMANITAS
} Milan, Italy

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Tue Nov 20 21:51:48 2001



From: reimar_gaertner-at-wsib.on.ca ()
Date: Tue, 20 Nov 2001 21:34:01 -0600
Subject: Ask-A-Microscopist: What is this marvelous thing?

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (reimar_gaertner-at-wsib.on.ca) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday,
November 19, 2001 at 14:48:41
---------------------------------------------------------------------------

Email: reimar_gaertner-at-wsib.on.ca
Name: Reimar Gaertner

Organization: WSIB

Education: Graduate College

Location: Toronto, Ontario, Canada

Question: My daughter and I have come across a most curious
pond-water creature that we can't identify in any of our books.
It is large - about 400 um in diameter and round.
It has movable internal organs that move food around - especially two
pincer-like claws that appear to be macerating captured food.
It therefore must be an animal - no cilia are apparent.
It has a unique method of feeding: it unfolds a clear
satalite-dish-like catchers mit with which it directs passing prey
into it's mouth area.
It can quickly retract the antena array when touched or jolted.
What is this marvelous thing?

---------------------------------------------------------------------------


From daemon Tue Nov 20 21:51:49 2001



From: elir-at-chem.ch.huji.ac.il ()
Date: Tue, 20 Nov 2001 21:32:28 -0600
Subject: Ask-A-Microscopist: correct setup for a wide illumination in a

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (elir-at-chem.ch.huji.ac.il) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
November 18, 2001 at 13:55:49
---------------------------------------------------------------------------

Email: elir-at-chem.ch.huji.ac.il
Name: Eli rothenberg

Organization: Hebrew University

Education: Graduate College

Location: Jerusalem, Israel

Question: I would like to know what is the correct setup for a wide
illumination in a confocal microscopy arrangement, that is, what
optics should i use in the path of the laser, if i want to get the
maximum illuminated area through my objective,
the setting is as follows: laser-collimated beam-
dichroic mirror-objective-illuminated surface.
(p.s I thought of using a short focusing lens that will focus half
way through the objective so maybe that will give me a wide
illumination on the surface, and/or maybe a diffuser to go along with
it?)

Thanks

Eli

---------------------------------------------------------------------------


From daemon Tue Nov 20 21:51:48 2001



From: malone1527-at-aol.com ()
Date: Tue, 20 Nov 2001 21:33:03 -0600
Subject: Ask-A-Microscopist: Balsam still considered a good mounting

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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (malone1527-at-aol.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
November 18, 2001 at 19:17:02
---------------------------------------------------------------------------

Email: malone1527-at-aol.com
Name: Gene Cimino

Organization: None-Microscopy is a hobby for me. I am 58 years old

Education: Undergraduate College

Location: Hydes,MD,USA

Question: Is Balsam still considered a good mounting medium?
If so, can it be kept fluid enough to apply by adding Xylol to it? I
am curious about this as I want to make some mounts of insect
structures. I know about Permount, but I wanted to try the older
Balsam method.By the way,I got my BS in Biology in 1967 from Towson
University.

Thanks,

Gene Cimino

---------------------------------------------------------------------------


From daemon Wed Nov 21 03:20:42 2001



From: f28130-at-id.ru
Date: Wed, 21 Nov 2001 05:44:37 -0500
Subject: STOCK EXPECTED TO SOAR 400%

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Reimar,

It sounds to me very much like a rotifer: these are very common, and can
survive considerable desiccation, and so are often found in roof gutters
(where I remember finding them). I would guess that the "satellite
dish" is the ciliated "wheel" from which the phylum gets its name - I
think that the cilia are either moving too fast or simply too small to
be seen at whatever magnification you are using. This generates a
current, and I remember as a boy watching green spherical algae being
sucked in and macerated.

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+

----- Original Message -----
} From: {reimar_gaertner-at-wsib.on.ca}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, November 21, 2001 3:34 AM


Biomasse International, Inc. (OTCBB BIMS)

Immediate & Strong BUY Recommendation

Huge Analyst and Newletter Coverage for BIMS

BIMS will soon be profiled by some major analysts
and newsletters along with the release of significant
news regarding explosive sales for the Company. There
will be huge volume and a strong increase in price for
several days. The same groups that featured TIWI will
begin coverage on BIMS. TIWI exploded from $ .44 to
$2.41 in 4 days!! We know for certain that the same
groups are going to feature BIMS and even better returns
are expected.

We are very proud that we can share this information with
you so that you can make a profit out of it. It is highly
advisable to take a position in BIMS as soon as possible,
today before the market closes or tomorrow.

The stock could easily reach $5.00 in less than a month on
the strength of their upcoming contract announcements and
Strong Analyst Buy Recomendations.

When word gets out this stock will SOAR!



From daemon Wed Nov 21 06:56:31 2001



From: pedromiguel.rodriguesdealmeida.pr-at-belgium.agfa.com
Date: Wed, 21 Nov 2001 06:46:04 -0600
Subject: TEM on ink-jet materials (dyes, pigments)

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Dear Colleagues,

I am trying to find out what has been recently published in the field
of ink-jet
materials (dyes, pigments) using transmission electron microscopy. If you know
of any reviews or research articles on this specific subject I would appreciate
your feedback!

Thank you very much,

Pedro Miguel Rodrigues de Almeida, Dr. Sc.
Agfa-Gevaert N.V.
RDM/PA-Mo - Electron Microscopy Laboratory
Septestraat 27
BE-2640 Mortsel

t +32 (0)3 444 3702
f +32 (0)3 444 3709


From daemon Wed Nov 21 08:34:34 2001



From: Melina Meli :      applina-at-libero.it
Date: Fri, 01 Jan 1904 06:09:58 +0000
Subject: EDS Microanalysis

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear expert microscopist,
I'm a very beginner with SEM imaging and particularly with EDS
Microanalysis. I'm trying to work with my own standard (Oxford ME-....)
and the standard-loading job is not quite simple for me. I've got I few
questions tha you might find stupid but I would appreciate any help!!!
1) The SEM is a LEO 4400
2) I work with geological sample
3) The EDS software package is ISIS 300
4) What are the best conditions for loasing the standard? i.e. 15 or 20
Kv EHT? 600 ma I-Probe or more? 50 or 100 sec. Preset Time? I have
mostly pure metals and compounds plus Natural Albite, Ortoclase and
Wollastonite.
5) Once I've performed the calibration using Cobalt, must I fix the
counts per second to the same value I obtained for the Cobalt for any
further standard acquisition?
6) Before preparing the element profiles, what's the best way to strip
peaks away from the spectrum: basic strip or advanced strip? This is
particularly important for silicates where Si, Al and Na peaks are close
(i.e. whithin the -600/+600 ev energy windows).
7) How can I perform deconvolution of peaks (Si and Al) before saving
the profiles? Is it possible to "erase" unwanted peaks from the standard
spectrum without altering the background?
Thank You for any answer or suggestion.
I will appreciate.
Melina Meli




From daemon Wed Nov 21 23:45:42 2001



From: Joanne Colmer :      joanne.colmer-at-adelaide.edu.au
Date: Thu, 22 Nov 2001 15:59:53 +1030
Subject: SEM - removing specimens from stubs

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22 Nov 2001 16:00:22 +1030
Message-ID: {3BFC8D51.E5B293F-at-adelaide.edu.au}


Hi all,

I have been asked to remove a specimen from a sem stub turn it over and
photograph the other side.
The specimen is an organically preserved plant fossil and very fragile,
it is attached to the sem stub using double sided black tape called STR
tape made by Shinto Chemitron Co. Ltd.
I have tried soaking a piece of the tape in acetone and also in ethanol
to see if this would dissolve the tape.
Neither did anything and the tape is still sticky.
Any suggestions would be greatly appreciated

Jo Shrapnel
Environmental Biology
Adelaide University


From daemon Thu Nov 22 02:18:57 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Thu, 22 Nov 2001 03:10:18 -0500
Subject: Another kind of "powers of 10"

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Dear Listers,
As my wife and I drove and hiked around the Northeast one last time to
see the fall colors before driving across from New York to California, I
thought of the differences among what could be seen while walking, driving,
and flying. It occurred to me that these modes of transportation differed
in rates by roughly factors of ten: a brisk walk is about 2 m/s; a
leisurely drive is about 20 m/s; an airliner travels at about 200 m/s. As I
thought further, I realized that a weather satellite at a height of about
1000 km would have an orbital speed of about 2 km/s, and scanning through a
TEM grid looking for suitably crystallized outer mitochondrial membranes
would happen at a speed of about 2 um/s. (I can examine the ~10 cm screen
at 40 kx in about 1 s.). Also, a trip across the solar system at 0.01 c
would take about a day. Other possibilities for filling in this series
could be the scan speed of AFM on one end and the rate that the Hubble
telescope can record a field for the deep field survey at the other.
(Someone knowledgeable in astronomy could calculate the apparent speed by
figuring how much distance is represented by the angular width of the Hubble
field of view at a depth of 10^10 light-years and dividing by the exposure
time.) Can we put together a complete list of powers-of-ten speeds, along
with how these can be realized and what typically can be seen? I'll start.

?? AFM individual atoms
2 um/s TEM organelles
2 m/s walk individual rocks, trees, streams, etc.
20 m/s drive mountains, lakes, forests, etc.
200 m/s fly mountain ranges, storms, deserts, etc
2 km/s orbit weather patterns, continents
200 Mm/s ion drive? solar system
?? Hubble scan quasar field
I hope you will have as much fun with this as I have.
Yours,
Bill Tivol




From daemon Thu Nov 22 03:35:28 2001



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 22 Nov 2001 03:28:11 -0800
Subject: SEM - removing specimens from stubs

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I'm not familiar with that particular product, but adhesives that remain
sticky (rather than dry out) usually respond very well to naptha. Lighter
fluid for zippo type cigarette lighters contains naptha and is an
inexpensive and readily available source.



Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


-----Original Message-----
} From: Joanne Colmer [SMTP:joanne.colmer-at-adelaide.edu.au]
Sent: Wednesday, November 21, 2001 9:30 PM
To: Microscopy-at-sparc5.microscopy.com


Hi all,

I have been asked to remove a specimen from a sem stub turn it over and
photograph the other side.
The specimen is an organically preserved plant fossil and very fragile,
it is attached to the sem stub using double sided black tape called STR
tape made by Shinto Chemitron Co. Ltd.
I have tried soaking a piece of the tape in acetone and also in ethanol
to see if this would dissolve the tape.
Neither did anything and the tape is still sticky.
Any suggestions would be greatly appreciated

Jo Shrapnel
Environmental Biology
Adelaide University



From daemon Thu Nov 22 06:07:00 2001



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 22 Nov 2001 12:28:02 +0000
Subject: Re: SEM - removing specimens from stubs

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Dear all
Most photographers are only too aware that flash intensity
diminishes according to the square of the distance to the subject,
making it difficult to use flash for distance photogrpahy with a lens
or telescope.
Some years ago I considered the possibility of illuminating a
subject viewed with a telescope or telephoto lens by using the
same optics to focus an electronic flash on the subject. A problem
was how short would the flash duration have to be for the all
illuminating light to have left the optics before image-forming light
started to come back. I concluded that for an object distance of
50m, i.e. a total light-path of 100m, would be about a third of a
microsecond, and gave up at that point, having no way of
generating flashes that short.

An orders of magnitude table (in 3-orders of magnitude steps)
for distance travelled by a light flash looks like this:
1 second 300,000 km
1 millisecond 300 km (this is typical electronic flash duration)
1 microsecond 300 m
1 nanosecond 0.3 m
1 picosecond 0.3 mm
1 femtosecond 0.3 µm (30nm)

so a light "beam" of 1 femtosecond duration, such as can be
produced by lasers, is in fact a light disc!

Not a lot of people know that :-)
Best wishes
Chris



Date sent: Thu, 22 Nov 2001 03:10:18 -0500


Joanne

Chloroform appears to soften our sticky tape and its adhesive within a
few minutes - if you have any spare STR tape you could try it first. It
should be easy to separate the sample then. However I don't know
whether chloroform will damage your sample, you will probably need to
rinse the surface of the sample with fresh solvent and remember to use
the chloroform in the fume hood.

Malcolm Haswell
e.m. unit,
School of Sciences,
University of Sunderland,
UK
(+44)0191 515 2872

Joanne Colmer wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all,
}
} I have been asked to remove a specimen from a sem stub turn it over and
} photograph the other side.
} The specimen is an organically preserved plant fossil and very fragile,
} it is attached to the sem stub using double sided black tape called STR
} tape made by Shinto Chemitron Co. Ltd.
} I have tried soaking a piece of the tape in acetone and also in ethanol
} to see if this would dissolve the tape.
} Neither did anything and the tape is still sticky.
} Any suggestions would be greatly appreciated
}
} Jo Shrapnel
} Environmental Biology
} Adelaide University


From daemon Thu Nov 22 07:15:17 2001



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Thu, 22 Nov 2001 13:07:09 +0000 (GMT)
Subject: Confocal applications review paper

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Dear Colleagues,
A colleague of mine, working on a thesis in pathology
is looking for review papers on the application of
confocal microscopy in cancer research, diagnosis and
prognosis. Can anyone cite useful recent references
for him, or possesses or has authored a favored
reference?
many thanks,
Jeremy Sanderson

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From daemon Thu Nov 22 10:36:47 2001



From: Steve Thomas :      THOMASS-at-biosrv1.bham.ac.uk
Date: Thu, 22 Nov 2001 16:28:19 -0000
Subject: ESEM & Colloidal gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

First posting to the list. I originally posted this on Sci.techniques.microscopy but was advised
that better response may be obtained here.
I'm a post doc reseacher at the Uni of B'Ham, UK.

I was wondering whether anybody had any experince of using colloidal
gold in ESEM. I wish to visualise fungal extracellular matrices released
onto glass or plastic surfaces in "wet" mode. If any body has any advice
on this, or has used colloidal gold in ESEM and can pass on a few tips
it would be most appreciated.

Regards

Steve Thomas

*********************************
Dr Steve Thomas
School Of Biosciences
The University of Birmingham
Edgbaston
Birmingham
B15 2TT
0121 414 5573
Email: S.Thomas-at-bham.ac.uk


From daemon Thu Nov 22 15:37:44 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 22 Nov 2001 13:58:22 -0500
Subject: SEM - Removing carbon tape adhesive from fragile samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jo Shrapnel wrote:
=======================================================
I have been asked to remove a specimen from a sem stub turn it over and
photograph the other side.
The specimen is an organically preserved plant fossil and very fragile,
it is attached to the sem stub using double sided black tape called STR
tape made by Shinto Chemitron Co. Ltd.
I have tried soaking a piece of the tape in acetone and also in ethanol
to see if this would dissolve the tape.
Neither did anything and the tape is still sticky.
Any suggestions would be greatly appreciated
========================================================
The adhesive system will not really "dissolve" in any common solvents. It
can be swollen and loosened, such as in chloroform, which can aid in its
removal, but it will not actually be dissolved away in the traditional sense
. For a flat surface often times such solvent treatment is enough but for
your kind of sample, I would trust it would not be enough.

But in a sense you are fortuante in that your sample of interest is not
organic but is in fact inorganic in nature. You should be able to put it
into a small glass petri dish, without cover, contaminating adhesive side
"up", and then using an oxygen plasma, such as would be generated in the SPI
Supplies® Plasma Prep™ II plasma etcher, the organics should be etched away
and the substrate (relatively) untouched. I say "relatively" because
eventually, in the limit, the oxygen will cause changes in the substrate,
but the etch rate for the organic adhesive is many times faster than for the
inorganic substrate. So you want to be careful to etch only to the extend
you need to etch and to try not etching beyond that point.

Special note and disclaimer: SPI Supplies manufactures the Plasma Prep II
plasma etcher so we would naturally have a vested interest in expanding its
use. However, the same physics found in the SPI Plasma Prep II unit also
exist in several other table-top systems being manufactured such as by
Denton Vacuum and Polaron (Now Quorum Technologies, formerly, VG Microtech).
More about the Plasma Prep II plasma etcher can be found at URL
http://www.2spi.com/catalog/instruments/etchers1.html


Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


From daemon Thu Nov 22 16:44:03 2001



From: gaydm :      gaydm-at-uncwil.edu
Date: Thu, 22 Nov 2001 17:20:48 -0500
Subject: Online journal help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are trying to locate a journal in the medical or cellular fields with an
online version that accepts videoclips. Thank you in advance for your help.

D. Mark Gay
UNC Wilmington
gaydm-at-uncwil.edu




From daemon Thu Nov 22 17:18:05 2001



From: Mark G BLACKFORD :      mgb-at-ansto.gov.au
Date: Fri, 23 Nov 2001 10:11:34 +1100
Subject: Leonid meteor report

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

as there was a little interest in the Leonid meteors on this list server
last week I thought would report my observations from Ayers Rock, Australia.


Central Australia was expected to be reasonably placed to observe the peak
activity early on Monday morning, Nov 19th. The sky was clear and dark from
sunset to sunrise and relatively warm (about 16°C). From the sand dunes I
spent a very pleasant 5 or 6 hours under the stars and counted approximately
300 meteors in the first 90 minutes. After that the rate was too great to
keep count. I estimate seeing approximately 2000 to 3000 meteors, with many
others that I missed because I was looking the wrong way at the time. I can
well imagine whiplash injuries being commonplace amongst those of us
fortunate enough to be in the right place at the right time with the right
weather conditions.

By comparison, the next morning I saw only 8 meteors in 90 minutes, and only
2 of these were Leonids.

Nestor, I managed to capture a few meteors in photos (one 15 minute exposure
has over 20 meteors visible on the print). I'll bring some of the better
ones to Adelaide. Cheers,

Mark Blackford
Materials Division, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.



From daemon Fri Nov 23 12:11:02 2001



From: m.andersson-at-t-online.de (Maike Andersson)
Date: Fri, 23 Nov 2001 18:58:54 +0100
Subject: Freeze substitution procedures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: Maike Andersson [m.andersson-at-t-online.de]
Sent: Mittwoch, 14. November 2001 21:27
To: Microscopy


I am trying to characterize the water pathway in plant tissue
by the water soluble, apoplastic tracer sulforhodamin.
In order to prevent secondary movement of the tracer, I want to
freeze substitute under anhydrous conditions prior to embeddment
in Spurr.
What substitution media do you recommend?
Does polar acetone lead to loss or secondary movement of the tracer?
Is apolar diethylether a better choice and how can I overcome
the very slow rewarming protocols of it?
Thanks in advance

Maike Andersson
Institut for Applied Botany
Hamburg / Germany


From daemon Fri Nov 23 13:59:20 2001



From: Jon Mcgovern :      jmcgover-at-ucalgary.ca
Date: Fri, 23 Nov 2001 13:49:32 -0600
Subject: [Fwd: Position Available]: University of Calgary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Listers;
The University of Calgary has a terrific job opening in the Microscopy
and Imaging Facility.

Please check it out. Calgary is one of the most prime cities to live
in. We are 70 miles from Banff and the best mountain activities in the
world. Our province has no sales tax!

Because of government regulations we must first look at qualified
Canadian citizens or Landed Immigrants and then folks from other
nations. We of course are an equal opportunity employer.

Jon McGovern
Manager, Microscopy and Imaging Facility
University of Calgary
Calgary, Alberta, Canada.


Position Available

The Microscopy and Imaging Facility of the University of Calgary
requires an experienced EM technologist.
The Microscopy and Imaging facility houses three transmission and three
scanning electron microscopes. The flagship of these being a newly
acquired FEI/Philips Tecnai F20 equipped with EDX, STEM, EELS, and Cryo
imaging. This instrument is the only one of its kind in Canada.
The successful candidate should have a very strong background in TEM,
preferably with cryo microtomy and cryo observation. Experience in
operational instruction to a wide group of users is a necessity.
Overseas training on the Tecnai F20 will be provided.
It is expected that the successful candidate will assist with the SEM
side of the facility as required. Although there are no strict
educational requirements, candidates should possess the appropriate
qualifications and training for the position. Experience in operating
EM’s, routine maitenance, management of the day-to-day operation of the
instruments, the ability to instruct new users, and work in a multi-user
environment would be an asset. This position will appeal to persons who
thrive on challenges and are willing to take on demanding tasks.
Applicants are invited to forward their resumes, three letters of
reference and if possible examples of their expertise to:

Dr. John Reynolds (reynolds-at-ucalgary.ca)
Head, Department of Cell Biology and Anatomy
Faculty of Medicine
University of Calgary
3330 Hospital Dr NW
Calgary, Alberta
Canada T2N 1N4


From daemon Fri Nov 23 16:07:30 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 23 Nov 2001 15:58:14 -0600
Subject: ESEM & Colloidal gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Steve,

I've never looked at colloidal gold in an ESEM before, although I've imaged
it in a FESEM, but my immediate reaction is that you would have two things
working against you. One is that to image a wet or uncoated sample, you
would have to introduce sufficient gas into the chamber to seriously degrade
your resolution. The second is that you need to use the backscatter
detector to image the gold particles, as you know, which again is
significantly lower resolution than the secondary imaging mode. With these
two resolution-losers combined, I seriously doubt that you could image gold
particles down into the nanometer range.

But, that said, I always tell people that only way to really know for sure
is to try. But, is there some compelling reason why the sample must be
viewed in "wet" mode, or for that mattter, in an SEM at all? Is there a
reason not label and embed your samples for TEM?

Good luck,
Randy Tindall
EM Core
University of Missouri
Columbia, MO



-----Original Message-----
} From: Steve Thomas
To: Microscopy-at-sparc5.microscopy.com
Sent: 11/22/01 10:28 AM


Hello,

First posting to the list. I originally posted this on
Sci.techniques.microscopy but was advised
that better response may be obtained here.
I'm a post doc reseacher at the Uni of B'Ham, UK.

I was wondering whether anybody had any experince of using colloidal
gold in ESEM. I wish to visualise fungal extracellular matrices released
onto glass or plastic surfaces in "wet" mode. If any body has any advice
on this, or has used colloidal gold in ESEM and can pass on a few tips
it would be most appreciated.

Regards

Steve Thomas

*********************************
Dr Steve Thomas
School Of Biosciences
The University of Birmingham
Edgbaston
Birmingham
B15 2TT
0121 414 5573
Email: S.Thomas-at-bham.ac.uk


From daemon Sat Nov 24 16:09:08 2001



From: bob-at-rockisland.com ()
Date: Sat, 24 Nov 2001 15:47:46 -0600
Subject: Ask-A-Microscopist:Info needed Vickers M171237

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bob-at-rockisland.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Saturday,
November 24, 2001 at 15:06:36
---------------------------------------------------------------------------

Email: bob-at-rockisland.com
Name: Bob Carter

Organization: independant researcher

Education: Graduate College

Location: Lopez Island WA 98261 USA

Question: Hi. I recently purchased a Vickers M171237 (Patholux???).
It is a compound "research " type similar to the Leitz Orthomat. It
has a quick change 5 objective nose, digital readout, substage and
epi lighting, plus a box under the binocular with a beamsplitter,
pol, and other optics I am unfamiliar with. I am restoring my
Vickers for daily usage. I am looking for technical information, and
user or repair guides. I completely disassembled, cleaned,
reassembled, and adjusted an AO MicroStar, but this microscope is far
more complicated. If you know of any resources, it would be greatly
apreceated. Thanks, Bob

Bob Carter
2000 Bayshore Road
Lopez Island, WA USA 98261



---------------------------------------------------------------------------


From daemon Sun Nov 25 19:01:54 2001



From: CHSal-at-aol.com
Date: Fri, 23 Nov 2001 14:12:38 -0500
Subject: Chickweed Healing Salve

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chickweed Healing Salve


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Testimonial: I had places on my face that were diagnosed as skin cancer. I saw an ad in the paper on Chickweed Healing Salve. I decided it was worth a try. I used it morning and night for about two weeks. Now I use it at night. The places are almost gone. I have used it faithfully since June 6th, 2001. I wouldn't be without it. Shirley Davis New Washington, IN 812-293-3555
Testimonial: My newly born baby, now one month old, had chronic diaper rash. Several doctors had tried to get it to go away...nothing seemed to work, she continued to be raw and sore. She cried all the time. I tried your Chickweed Healing Salve, putting it on that night. By the next morning she was much better. After using it for 2 days, she was completely cured. She is now a beautiful baby. She doesn't cry anymore. I paid $19.95 for it, however I would gladly have paid $100. Thanks so much. I will always keep it on hand. I will gladly give your product a good reference if anyone wants to give me a call. Shellie Cooper Stanly, KS 913-402-0411
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From daemon Sun Nov 25 19:59:23 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 25 Nov 2001 17:59:28 -0800
Subject: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all:

Is there any direct way to quantitatively determine
and measure the actual spot size of a SEM beam?

Given various KV, WD, final aperture sizes, condenser lens
settings, etc., is there a way to truly find out what
the real spot size is on the specimen?

Any ideas?

gary g.



From daemon Mon Nov 26 07:14:58 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Mon, 26 Nov 2001 08:04:17 -0500
Subject: RE: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

In TEM we measure the actual contamination spot "burned"
into the carbon film by the beam. I use a grid with
calibration spheres for reference. With the appropriate
organic film, a similar technique could work with SEM in
spot mode?? I'm not sure how much accuracy you're looking
for, but it might give you an idea.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Sunday, November 25, 2001 8:59 PM, Gary Gaugler
[SMTP:gary-at-gaugler.com] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} Hi all:
}
} Is there any direct way to quantitatively determine
} and measure the actual spot size of a SEM beam?
}
} Given various KV, WD, final aperture sizes, condenser
} lens
} settings, etc., is there a way to truly find out what
} the real spot size is on the specimen?
}
} Any ideas?
}
} gary g.



From daemon Mon Nov 26 08:00:00 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Mon, 26 Nov 2001 08:53:07 -0500
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
One technique I've seen used is to mount an aperture over a hole (on
carbon is best), or better yet, a thinned foil for a sharper edge, and
use a line-profile mode to scan across after focussing at a very high
mag. Absorbed current can be used instead of secondary for a signal,
although the latter works well, especially on a thinned foil. The curve
you get will resemble a rise-time curve and can be treated in a similar
manner. Mark the 10% and 90% rise points on the curve, then measure the
horizontal distance between those two points in units appropriate for
the mag. This is generally considered to be the beam diameter given the
Gaussian distribution of electrons across the beam.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all:
}
} Is there any direct way to quantitatively determine
} and measure the actual spot size of a SEM beam?
}
} Given various KV, WD, final aperture sizes, condenser lens
} settings, etc., is there a way to truly find out what
} the real spot size is on the specimen?
}
} Any ideas?
}
} gary g.
}
}
}
}



From daemon Mon Nov 26 08:05:17 2001



From: twiinie-at-aolcom ()
Date: Mon, 26 Nov 2001 07:57:57 -0600
Subject: Ask-A-Microscopist: old Bushnell Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (twiinie-at-aolcom) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
November 25, 2001 at 22:36:35
---------------------------------------------------------------------------

Email: twiinie-at-aolcom
Name: Brett Chamberlin

Education: 6-8th Grade Middle School

Location: Gresham, Oregon USA

Question: We recently purchased a fairly old Bushnell Microscope. It
is all metal construction with a service sticker. The service sticker
reads a last serviced date of 1986.

Where might I find a manual and/or some instruction on the features
of this microscope. There are some attachments that I do not
understand the function of.

It says "Magna" on the front of it.

Thank you very much for any help that you can offer.



---------------------------------------------------------------------------


From daemon Mon Nov 26 08:22:21 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 27 Nov 2001 00:17:29 +1000
Subject: RE: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any calculation would require factors specific for any one make of instrument.
Anyway, doing it experimentally is more fun. Microanalysts need to know the
actual location of the spot on the specimen and for that observe the beam spot
on a daily basis. For WDS an SEM needs among other accessories a light
microscope. With that and with the beam on a fluorescent mineral the beam spot
is readily visible.

Usually in SEM the spot is busy scanning, so spot mode is required and for that
the SEM scan coils are switched off. (Also, turn down fluorescent screen/
monitors, to avoid burn-marks. With a graticule in the eyepiece and micron-
step stage movements the beam can be measured, but . . . For small spots,
suitable for SEM the microscope on a microprobe will not have enough power.

The alternative is to burn spots onto a smooth specimen in a location which
could be found under a high power light microscope. A grided coverslip with a
light carbon film may be suitable. By parking the beam spot near line
intersections the burn mark could later be found and measured.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, November 26, 2001 11:59 AM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all:
}
} Is there any direct way to quantitatively determine
} and measure the actual spot size of a SEM beam?
}
} Given various KV, WD, final aperture sizes, condenser lens
} settings, etc., is there a way to truly find out what
} the real spot size is on the specimen?
}
} Any ideas?
}
} gary g.



From daemon Mon Nov 26 08:22:22 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 26 Nov 2001 06:20:47 -0800
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken:

This method has promise....it is not too difficult to do!
Sounds sort of like a Faraday cup affair. I could try
this with 35u, 70u and 100u apertures mounted on
a Carbon stub.

In general terms, what IS a typical spot size? Is it
Angstroms, microns, or what? I never really thought
about the true spot size--only relative, based on CL setting.
Higher setting, smaller spot size. If a spot is used with
the SEM in spot mode, the issue is to find out how big
the spot actually is. This affects spillover of data when
doing x-ray probing of very thin layers in microchips.

thanks to all,
gary g.


At 05:53 AM 11/26/2001, you wrote:
} Gary,
} One technique I've seen used is to mount an aperture over a hole (on
} carbon is best), or better yet, a thinned foil for a sharper edge, and use
} a line-profile mode to scan across after focussing at a very high
} mag. Absorbed current can be used instead of secondary for a signal,
} although the latter works well, especially on a thinned foil. The curve
} you get will resemble a rise-time curve and can be treated in a similar
} manner. Mark the 10% and 90% rise points on the curve, then measure the
} horizontal distance between those two points in units appropriate for the
} mag. This is generally considered to be the beam diameter given the
} Gaussian distribution of electrons across the beam.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
} Gary Gaugler wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Nov 26 09:17:14 2001



From: Ken Bart :      kbart-at-hamilton.edu
Date: Mon, 26 Nov 2001 10:09:24 -0500
Subject: Flatbed scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:
I am looking to replace my old Epson flat bed scanner and
want a high quality scanner. This instrument is used primarily for
scanning TEM negatives, 35 mm slides, some OCR and needs to be
function in a multi-user environment. I have been considering the
UMAX Powerlook 3000 and the Heidelberg Linoscan 2650 due to their
reported 3.6 dynamic range and 42 bit pixel depth. Does anyone have
experience with these machines? Is there something better?

Thanks!

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/


From daemon Mon Nov 26 10:54:27 2001



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 26 Nov 2001 08:45:29 -0800
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gary,
The classic method is to take the highest magnification photo that will show
you the resolution limit, defined as the closest two points can be and still
be determined as two points instead of one. I just take a hi-mag photo of a
highly detailed surface and measure the smallest detail I can see and divide
by the magnification. This will be the resolution at the conditions
currently set. I was always told that the resolution of the SEM was equal to
the diameter of the electron beam. For a sample I use an evaporated gold
film on a graphite substate, to reduce the contribution of back-scattered
electrons.
At 05:59 PM 11/25/01 -0800, you wrote:
}
} Hi all:
}
} Is there any direct way to quantitatively determine
} and measure the actual spot size of a SEM beam?
}
} Given various KV, WD, final aperture sizes, condenser lens
} settings, etc., is there a way to truly find out what
} the real spot size is on the specimen?
}
} Any ideas?
}
} gary g.
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchange.ubc.ca



From daemon Mon Nov 26 11:22:13 2001



From: Michael Herron :      herro001-at-umn.edu
Date: Mon, 26 Nov 2001 11:15:10 -0600
Subject: Whole body fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

I am using a stereo microscope equipped for epiflourescent GFP
excitation and emission. I am looking at live GFP transgenic mice and
while the technique works quite well, there is a problem with
autoflourescence of the skin surface.

These are mouse pups with little or no hair, and the skin has dander or
flakes that seems to autofluor. I was wondering if anyone might know of
a solution I could quench this external autofluor with? Something mild
would be in order since these are delicate little critters.

Thanks,
Mike

--

________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001-at-umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/


From daemon Mon Nov 26 11:56:16 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 26 Nov 2001 10:19:34 -0600
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have _usually_ assumed for purposes of x-ray analysis that the SEM spot
size is zero. I know it is not, but considering the interaction volume
inside the specimen, the beam size is almost negligible. The various Monte
Carlo programs tell me that I must consider an interaction volume on the
order of a micron or more at 15 kV depending on my substrate. I am pretty
sure that my beam is not half a micron in diameter, so how much will it
affect the interaction volume?

I suppose the experiment to try would be to use an x-ray signal along with
the secondary or absorbed current signal to check the effective spot size.
And I suppose I should check the backscattered signal too while I am at it.

"Fortunately", much of my work has not involved the small structures where
we are striving to keep the beam within the very thin layers. But those
problems are coming along with increasing frequency. One of these days I
will probably need to delve into the deconvolutions needed to determine the
compositions of phases smaller than the excitation volume. I recall seeing
an example of principle component analysis at MSA (2000?) where spectrum
imaging allowed the discernment of a thin phase at an interface that was
ordinarily undiscernible. Of course I do not have the program or equipment
that those folks at Oak Ridge and NORAN had, so it will be a while before I
could repeat the experiment. I wonder if anyone has developed a good,
public-domain version of the routine.

Warren

At 06:20 AM 11/26/01 -0800, Gary wrote:

} Ken:
}
} This method has promise....it is not too difficult to do!
} Sounds sort of like a Faraday cup affair. I could try
} this with 35u, 70u and 100u apertures mounted on
} a Carbon stub.
}
} In general terms, what IS a typical spot size? Is it
} Angstroms, microns, or what? I never really thought
} about the true spot size--only relative, based on CL setting.
} Higher setting, smaller spot size. If a spot is used with
} the SEM in spot mode, the issue is to find out how big
} the spot actually is. This affects spillover of data when
} doing x-ray probing of very thin layers in microchips.
}
} thanks to all,
} gary g.
}
}
} At 05:53 AM 11/26/2001, you wrote:
} } Gary,
} } One technique I've seen used is to mount an aperture over a hole (on
} } carbon is best), or better yet, a thinned foil for a sharper edge, and
} } use a line-profile mode to scan across after focussing at a very high
} } mag. Absorbed current can be used instead of secondary for a signal,
} } although the latter works well, especially on a thinned foil. The curve
} } you get will resemble a rise-time curve and can be treated in a similar
} } manner. Mark the 10% and 90% rise points on the curve, then measure the
} } horizontal distance between those two points in units appropriate for the
} } mag. This is generally considered to be the beam diameter given the
} } Gaussian distribution of electrons across the beam.
} }
} } Ken Converse
} } owner
} } Quality Images
} } third party SEM service
} } Delta, PA
} }
} } Gary Gaugler wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Nov 26 12:19:51 2001



From: Dr. William G. Pitt :      pitt-at-byu.edu
Date: Mon, 26 Nov 2001 11:15:12 -0700
Subject: TEM: need a hydrophobic stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a hydrophobic heavy metal stain to stain the
hydrophobic core of micelles suspended in water. I need to view the
micelles with TEM. Non-water soluable hydrophobic drug molecules
partition rapidly into the core; and I suspect a hydrophobic heavy metal
such as a stable organo metallic would also, such as lead
ethylhexanoate. Any suggestions or experience you can share with me?

Bill Pitt
BYU



From daemon Mon Nov 26 12:21:03 2001



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Mon, 26 Nov 2001 13:15:31 -0500
Subject: Dimpling silver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We would like to dimple silver for TEM sample prep. When we used our
standard methods, it dimpled exceedingly slowly (perhaps because it is
soft).

Does anyone have experience in dimpling silver successfully?
--
..........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


From daemon Mon Nov 26 14:38:42 2001



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Mon, 26 Nov 2001 15:29:42 -0500
Subject: Dimpling silver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Is this a silver film on a substrate of some sort or is it a lump of Ag?
If it is a piece of silver why don't you electroplish it? Far fewer
artifacts.

Ron

Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
NOTE CHANGES: 1-845-892-2225 Office; -6899 FAX; 1-800-352-4732, pin 8604
Pager; 1-914-453-2917 Personal Cell Phone


Alwyn Eades {jae5-at-lehigh.edu} on 11/26/2001 01:15:31 PM

To: EMNET {microscopy-at-sparc5.microscopy.com}
cc:



We would like to dimple silver for TEM sample prep. When we used our
standard methods, it dimpled exceedingly slowly (perhaps because it is
soft).

Does anyone have experience in dimpling silver successfully?
--
.........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu






From daemon Mon Nov 26 16:33:48 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Mon, 26 Nov 2001 17:26:25 -0500
Subject: RE: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a good point; the interaction volume overwhelms the
actual "spot size" under most circumstances. But since
Gary asked for some actual numbers: In TEM (W filament) I
generally find the spot ~200nm or less by measuring the
contamination spot. As a matter of interest, I believe for
NVLAP asbestos analysis a spot {250nm is required. That
is, the *minimum* obtainable spot must be {250nm. Since,
as Ken mentioned, the distribution is presumed Gaussian
there is a bit of subjectivity as to where you pick the
"edge" and how long you burn....not to mention
time-dependent drift.

I have a table of "theoretical" spot sizes for my ESEM-FEG:
they range from {1nm to about 40nm depending on kV and
spot size selected.

Matt

On Monday, November 26, 2001 11:20 AM, Warren E Straszheim
[SMTP:wesaia-at-iastate.edu] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} I have _usually_ assumed for purposes of x-ray analysis
} that the SEM spot
} size is zero. I know it is not, but considering the
} interaction volume
} inside the specimen, the beam size is almost negligible.
} The various Monte
} Carlo programs tell me that I must consider an
interaction
} volume on the
} order of a micron or more at 15 kV depending on my
} substrate. I am pretty
} sure that my beam is not half a micron in diameter, so
how
} much will it
} affect the interaction volume?
}
} I suppose the experiment to try would be to use an x-ray
} signal along with
} the secondary or absorbed current signal to check the
} effective spot size.
} And I suppose I should check the backscattered signal too
} while I am at it.
}
} "Fortunately", much of my work has not involved the small
} structures where
} we are striving to keep the beam within the very thin
} layers. But those
} problems are coming along with increasing frequency. One
} of these days I
} will probably need to delve into the deconvolutions
needed
} to determine the
} compositions of phases smaller than the excitation
volume.
} I recall seeing
} an example of principle component analysis at MSA (2000?)
} where spectrum
} imaging allowed the discernment of a thin phase at an
} interface that was
} ordinarily undiscernible. Of course I do not have the
} program or equipment
} that those folks at Oak Ridge and NORAN had, so it will
be
} a while before I
} could repeat the experiment. I wonder if anyone has
} developed a good,
} public-domain version of the routine.
}
} Warren
}
} At 06:20 AM 11/26/01 -0800, Gary wrote:
}
} } Ken:
} }
} } This method has promise....it is not too difficult to
do!
} }
} } Sounds sort of like a Faraday cup affair. I could try
} } this with 35u, 70u and 100u apertures mounted on
} } a Carbon stub.
} }
} } In general terms, what IS a typical spot size? Is it
} } Angstroms, microns, or what? I never really thought
} } about the true spot size--only relative, based on CL
} } setting.
} } Higher setting, smaller spot size. If a spot is used
} } with
} } the SEM in spot mode, the issue is to find out how big
} } the spot actually is. This affects spillover of data
} } when
} } doing x-ray probing of very thin layers in microchips.
} }
} } thanks to all,
} } gary g.
} }
} }
} } At 05:53 AM 11/26/2001, you wrote:
} } } Gary,
} } } One technique I've seen used is to mount an aperture
} } } over a hole (on
} } } carbon is best), or better yet, a thinned foil for a
} } } sharper edge, and
} } } use a line-profile mode to scan across after focussing
} } } at a very high
} } } mag. Absorbed current can be used instead of
secondary
} } } for a signal,
} } } although the latter works well, especially on a thinned
} } } foil. The curve
} } } you get will resemble a rise-time curve and can be
} } } treated in a similar
} } } manner. Mark the 10% and 90% rise points on the curve,
} } } then measure the
} } } horizontal distance between those two points in units
} } } appropriate for the
} } } mag. This is generally considered to be the beam
} } } diameter given the
} } } Gaussian distribution of electrons across the beam.
} } }
} } } Ken Converse
} } } owner
} } } Quality Images
} } } third party SEM service
} } } Delta, PA
} } }
} } } Gary Gaugler wrote:
} } }
}
} } } -------------------------------------------------------
} } } } -----------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy
} } } } Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} } } }
}
} } } http://www.msa.microscopy.com/MicroscopyListserver/FA
Q.htm
} } } } l
}
} } } -------------------------------------------------------
} } } } ----------------.
} } } }
} } } } Hi all:
} } } } Is there any direct way to quantitatively determine
} } } } and measure the actual spot size of a SEM beam?
} } } } Given various KV, WD, final aperture sizes, condenser
} } } } lens
} } } } settings, etc., is there a way to truly find out what
} } } } the real spot size is on the specimen?
} } } } Any ideas?
} } } } gary g.
} }



From daemon Mon Nov 26 18:01:17 2001



From: Dick Windmiller :      dwind-at-exponent.com
Date: Mon, 26 Nov 2001 15:52:53 -0800
Subject: virtual SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to find information on remote control and remote viewing on the
SEM. I am currently trying to use Microsoft Net Meeting. Does anybody have a
better way to do these things?


From daemon Mon Nov 26 19:48:44 2001



From: Norton, Buddy :      hnorton-at-richmond.edu
Date: Mon, 26 Nov 2001 19:35:31 -0600
Subject: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there a reliable method of detecting Anthrax spores within a very short
time, through the use of Electron
Microscopy? Do you know anyone working with this method of detection of
Anthrax spores? The system I am looking into
involves using sticky pads to capture spores in work areas to include
heating and cooling vents. The material collected is then
taken to an area where a microscope is used to determine the presence or
no-presence of the spores. Within a few hours, 2-4
hours, there is a determination made if anthrax spores are in fact present.


$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$

"Buddy"

Howard B. Norton
Captain
Deputy Chief of Police - Operations

Office: 804-289-8723
Pager: 804-346-6263
Fax: 804-289-8720

hnorton-at-richmond.edu

31 UR Drive
Special Programs Building
University of Richmond Police Department
University of Richmond, Virginia 23173

For Sale - 1985 Prowler Camper Trailer 32'

Those who row the boat seldom have time to rock it.

$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$


From daemon Mon Nov 26 19:48:44 2001



From: zaluzec-at-microscopy.com
Date: Mon, 26 Nov 2001 19:31:56 -0600
Subject: Re: virtual SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is my "solution" to the electronic / virtual instrument problem
if you want so see something that is NOT netmeeting based.

http://tpm.amc.anl.gov

But there are other solutions. You should also look at

http://tpm.amc.anl.gov/Lectures/MM2000-M&MovertheNet.pdf

which also presents some other solutions. This is a pdf copy of the
slides I presented during a tutorial at the M&M2000 meeting.
That tutorial was video taped so if you are really keen you can
order a copy of the tape from the MSA Education Committee.

http://www.msa.microscopy.com/MSADocs/MSAVideotapeCatalogue95.html

Look for Tape #224... I've never seen the tape so I cannot comment on
quality or the content.

Cheers...

Nestor
Your Friendly Neighborhood SysOp
Disclaimer... I have a vested interest in the TPM site.
;-)


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Nov 26 19:50:47 2001



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 26 Nov 2001 17:28:10 -0800
Subject: Re: Ask-A-Microscopist: old Bushnell Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Email: twiinie-at-aolcom
} Name: Brett Chamberlin
}
} Education: 6-8th Grade Middle School
}
} Location: Gresham, Oregon USA
}
} Question: We recently purchased a fairly old Bushnell Microscope. It
} is all metal construction with a service sticker. The service sticker
} reads a last serviced date of 1986.
}
} Where might I find a manual and/or some instruction on the features
} of this microscope. There are some attachments that I do not
} understand the function of.
}
} It says "Magna" on the front of it.
}
} Thank you very much for any help that you can offer.
} ---------------------------------------------------------------------------

Brett -

If you can't find a manual, the general advice in this book will be very
helpful:

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

The description above was taken from the MICRO bibliography; you'll find
other helpful books there. URL below.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Nov 26 21:04:57 2001



From: RCHIOVETTI-at-aol.com
Date: Mon, 26 Nov 2001 21:57:09 EST
Subject: Re: Whole body fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 11/26/2001 10:23:39 AM US Mountain Standard Time,
herro001-at-umn.edu writes:

{ { I am using a stereo microscope equipped for epiflourescent GFP
excitation and emission. I am looking at live GFP transgenic mice and
while the technique works quite well, there is a problem with
autoflourescence of the skin surface.

These are mouse pups with little or no hair, and the skin has dander or
flakes that seems to autofluor. I was wondering if anyone might know of
a solution I could quench this external autofluor with? Something mild
would be in order since these are delicate little critters.
} }

Mike,

Is the autofluorescence yellowish? Rather than try and scrub a mouse pup, I
would first try filtering out the offending autofluor color with the scope,
assuming it probably isn't at exactly the same wavelength as the GFP signal.

It depends on your scope and what kind of filters you are using, but I would
contact either the manufacturer of the scope or the fluorescence rig and
inquire about narrow bandpass filters for GFP (both excitation and emission).
Some filter sets have longpass filters in them that pass the required
signals plus everything on either side for quite a distance out.

If you can get narrow bandpass filters, this should help a lot. If you
already have narrow bandpass filter in your scope...never mind the above
advice! I wonder how baby shampoo works on newborn mice...??

FYI, we have had good success using the advice of the folks at Chroma
Technology Corp., especially Paul Millman. You can contact Paul at Choma by
calling (800) 824-7662 in the U.S. They're experts at everything that's
fluorescence-related.

Good Luck!

Bob Chiovetti
GTI Microsystems
Tucson, AZ
Leica Exclusive Regional Dealers
(AZ, NM, West TX)


From daemon Tue Nov 27 00:03:22 2001



From: Lachlan McDonald :      lmcdonald-at-engeneic.com
Date: Tue, 27 Nov 2001 17:01:23 +1100
Subject: SEM - sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

Would appreciate some help with preparation of gram -ve bacteria for SEM.

I plan to do a standard gold coating but was wondering if anyone has some
practical experience on fixation before proceeding with the rest of the
protocol.

Many thanks in advance.

Lachlan

________________________
Lachlan McDonald
EnGeneIc Pty. Ltd.
105 Delhi Road
Riverside Corporate Park
North Ryde
NSW 2113
AUSTRALIA

Tel: (61-2) 9878-4945
Fax: (61-2) 9878-5922
_________________________



From daemon Tue Nov 27 03:13:42 2001



From: Jim Buckman :      Jim.Buckman-at-pet.hw.ac.uk
Date: Tue, 27 Nov 2001 09:06:44 -0000
Subject: LM Manual for Quantimet 570

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi,
}
} We are trying to salvage our 8 year old Cambridge Instruments Quantimet
570
} image analysis workstation. However there is no sign of any manuals.
Does
} anybody have a manual that they would be willing to photocopy for us,
} donate, orcan you put me in contact with someone who might have one??
}
} Any help appreciated,
}
} Jim
}
}
} Jim Buckman
} Department of Petroleum Engineering
} Heriot-Watt University
} Riccarton
} Edinburgh
} EH14 4AS
} Scotland
}
} jim.buckman-at-pet.hw.ac.uk



From daemon Tue Nov 27 07:46:38 2001



From: Dave Hall :      daveh-at-restechimage.com
Date: Tue, 27 Nov 2001 08:35:07 -0500
Subject: Flatbed scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken:

I don't have experience with either of the two scanners you mentioned.
For our customers, we usually recommend Microtek. We have had good
success with the (previously discussed here) ArtixScan 2500 which is
36-bit with a 3.4 dynamic range. Resolution is 2500 dpi (optical) and
the list price is $4,499. (Usually available for less.) (10,000
element CCD)

Microtek also offers (but I have yet to use) the ArtixScan 1100 which is
closer to the ones you mentioned. Optical resolution is 2000 x 1000,
42-bit, and a higher reported dynamic range of 3.9. This one has a list
price of $1,499. (8,000 element CCD)

Both of these use dual plate technology including Microtek's patented
Emulsion Direct Imaging Technology for incredibly sharp transparency
scanning. With E.D.I.T., there is no glass between the scanning lens,
film, or light source, eliminating distortion and Newton rings that
occur with traditional transparency adapters.

I hope this helps. If I can be of any additional assistance, please let
me know.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045



-----Original Message-----
} From: Ken Bart [mailto:kbart-at-hamilton.edu]
Sent: Monday, November 26, 2001 10:09 AM
To: microscopy-at-sparc5.microscopy.com


Hi:
I am looking to replace my old Epson flat bed scanner and
want a high quality scanner. This instrument is used primarily for
scanning TEM negatives, 35 mm slides, some OCR and needs to be
function in a multi-user environment. I have been considering the
UMAX Powerlook 3000 and the Heidelberg Linoscan 2650 due to their
reported 3.6 dynamic range and 42 bit pixel depth. Does anyone have
experience with these machines? Is there something better?

Thanks!

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/



From daemon Tue Nov 27 08:01:21 2001



From: Gillmeister, Russ :      RGillmeister-at-crt.xerox.com
Date: Tue, 27 Nov 2001 08:55:13 -0500
Subject: Whole body fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike, Why don't you try something like magic mending tape and do a few
strips on the areas of interest. The tape is quit good at cleaning surfaces
and it should be gentle enough and leave no residue. Good Luck,
Russ Gillmeister
Xerox
~~~~~~~~~~~~~


-----Original Message-----
} From: Michael Herron [mailto:herro001-at-umn.edu]
Sent: Monday, November 26, 2001 12:15 PM
To: microscopy-at-sparc5.microscopy.com


All,

I am using a stereo microscope equipped for epiflourescent GFP
excitation and emission. I am looking at live GFP transgenic mice and
while the technique works quite well, there is a problem with
autoflourescence of the skin surface.

These are mouse pups with little or no hair, and the skin has dander or
flakes that seems to autofluor. I was wondering if anyone might know of
a solution I could quench this external autofluor with? Something mild
would be in order since these are delicate little critters.

Thanks,
Mike

--

________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001-at-umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/


From daemon Tue Nov 27 09:28:17 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 27 Nov 2001 10:18:32 -0500 (EST)
Subject: Re: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Norton,

The answer is complex. Probably, the bottom line for practicality is
"no", not easily or rapidly. It could be done with the right equipment
and the right reagents.

Bacillis anthrasis spores morphologically resemble spores of other
Bacillus species (e.g., B. cereus). Some of these other bacilli are
ubiquitous (e.g., on food). Thus, it would be impossible to
differentiate, based solely on appearance, what species the spores were.

One possibility would be to react an antibody against B. anthrasis with
them and then tag that first antibody with a secondary antibody that had
a marker recognizable in the electron microscope. This could be done
either by scanning electron microscopy (SEM) of the surface of spores, or
by transmission electron microscopy (TEM) of ultrathin sections or of
negatively stained bacteria--although negative staining would not be
ideal because of the large size of the organism. (Negative staining is
usually used for small things like viruses).

This immunolabeling technique would not be particularly fast (a matter of
hours, rather than the days it would take to grow and speciate the
bacteria, but not minutes). It would also be rather cumbersome in
requiring immunological reagents and operators familiar with
immunolabeling and thin sectioning. It would also require that the
bacteria be handled as a biohazard and be killed before examination to
prevent contamination.

Another possibility for identification, or for at least preliminary
indication that malice was intended, would be to examine the preparation
with Xray microanalysis. Most times, weaponized B. anthrasis spores are
mixed with a chemical that causes them not to form large clumps. (The
smaller particle size allows them to get further into the small airways
of the lung.) This powder could be detected with microprobe analysis.
However, one could question whether a smart crook might use other kinds
of powder with different components to disguise the dispersant. Also,
one would need to kill the spores while working on them to prevent infection.

I hope this helps. While I¹m in the business of using the electron to
diagnose infectious diseases (we examine almost a thousand samples a year
for viruses), and welcome any new use, I want to acknowledge the
limitations.

Sara Miller


(P. S. The genus and species should be either italicized or underlined;
my primitive email program won¹t do this.)



Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




On Mon, 26 Nov 2001, Norton, Buddy wrote:

} Date: Mon, 26 Nov 2001 19:35:31 -0600
} From: Norton, Buddy {hnorton-at-richmond.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: detecting Anthrax spores using EM?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is there a reliable method of detecting Anthrax spores within a very short
} time, through the use of Electron
} Microscopy? Do you know anyone working with this method of detection of
} Anthrax spores? The system I am looking into
} involves using sticky pads to capture spores in work areas to include
} heating and cooling vents. The material collected is then
} taken to an area where a microscope is used to determine the presence or
} no-presence of the spores. Within a few hours, 2-4
} hours, there is a determination made if anthrax spores are in fact present.
}
}
} $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
}
} "Buddy"
}
} Howard B. Norton
} Captain
} Deputy Chief of Police - Operations
}
} Office: 804-289-8723
} Pager: 804-346-6263
} Fax: 804-289-8720
}
} hnorton-at-richmond.edu
}
} 31 UR Drive
} Special Programs Building
} University of Richmond Police Department
} University of Richmond, Virginia 23173
}
} For Sale - 1985 Prowler Camper Trailer 32'
}
} Those who row the boat seldom have time to rock it.
}
} $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
}
}
}





From daemon Tue Nov 27 09:49:59 2001



From: Peter Tomic :      PTomic-at-anadigics.com
Date: Tue, 27 Nov 2001 10:46:39 -0500
Subject: Infra-Red Thermal Imaging for Microelectronics; Contract Services

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists;

Our lab. had a failure of a thermal imaging system. The system is used for
thermal mapping integrated circuits. We are interested in using a contract
service to do measurements for us while our hardware is being repaired.
Does anyone know of a contract lab., preferably on the East Coast, that has
this capability for hire? I may consider a thermal AFM if available. The
system we have now is optical and low resolution compared to an AFM.
Resolution is ~ 10 uM.

Regards,
Peter Tomic
Failure Analysis & Analytical Services Group
Anadigics, Inc.
Warren, New Jersey



From daemon Tue Nov 27 10:36:14 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 27 Nov 2001 11:28:37 -0500
Subject: NESM Fall Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The New England Society for Microscopy (NESM) will be holding it's 35th
Annual Fall Symposium at UMASS-Lowell in Lowell, Massachusetts on Friday,
Dec. 7, 2001.

The meeting will begin at 12 Noon with Registration in the Mil Conference
Center-Wannalancit Building (North Campus).

There will be 3 Sessions: A special student session beginning at 1:05 pm
with 4 presentations, followed by a Poster session and coffee break.

Session II (Biological) begins at 2:20 pm and has as it's speaker, Ken
Moore (University of Iowa), the National MSA Speaker. His talk will
be: "Application of Microscopy Techniques to the Study of Genetic Therapy
Research".

Another Poster Session and Afternoon Coffee Break will follow at 3:20 pm.

Session III (Materials Science) will begin at 3:40 pm and have 2
speakers: Elen Humphreys from M.I.T. and Koichi Nishikida from Thermo
Spectra-Tech.

The Annual Business Meeting will convene at 5:00 pm and will include the
election of NESM officers for 2002.

A Dinner and after-dinner speaker will follow at 6 pm.

Advance registration is due by Friday, November 30th. Registration after
November 30th will NOT include dinner. The registration for NESM members
is $10.00 and $25.00 for non-members. Dinner is $15.00 extra.

For more detailed information, please contact Mary McCann, NESM Treasurer
at mccanns-at-tiac.net or NESM's website: The New England Society for
Microscopy (NESM) will be holding it's 35th Annual Fall Symposium at
UMASS-Lowell in Lowell, Massachusetts on Friday, Dec. 7, 2001.

The meeting will begin at 12 Noon with Registration in the Mil Conference
Center-Wannalancit Building (North Campus).

There will be 3 Sessions: A special student session beginning at 1:05 pm
with 4 presentations, followed by a Poster session and coffee break.

Session II (Biological) begins at 2:20 pm and has as it's speaker, Ken
Moore (University of Iowa), the National MSA Speaker. His talk will
be: "Application of Microscopy Techniques to the Study of Genetic Therapy
Research".

Another Poster Session and Afternoon Coffee Break will follow at 3:20 pm.

Session III (Materials Science) will begin at 3:40 pm and have 2
speakers: Elen Humphreys from M.I.T. and Koichi Nishikida from Thermo
Spectra-Tech.

The Annual Business Meeting will convene at 5:00 pm and will include the
election of NESM officers for 2002.

A Dinner and after-dinner speaker will follow at 6 pm.

Advance registration is due by Friday, November 30th. Registration after
November 30th will NOT include dinner. The registration for NESM members
is $10.00 and $25.00 for non-members. Dinner is $15.00 extra.

For more detailed information, please contact Mary McCann, NESM Treasurer
at mccanns-at-tiac.net or NESM's
website: http://www.msa.microscopy.com/MSALAS/NESM/NESMHome.htm






** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Tue Nov 27 10:50:42 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 27 Nov 2001 11:48:59 -0600
Subject: Re: Anthrax spore detection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Norton:

There is a SEM/EDS software "gunshot inspection" for particle inspection
automatically. I use this system for particle analysis on hard drive disk,
a 95 mm diameter disk with 0.5 um resolution in whole disk scaning. The
particle size range can be set so that the software ignores the larger one
or the smaller one (dirt), and locates the particles and does EDS analysis
automatically. I do not think you need to do EDS, but the image capture and
classification functions may be good enough for you.

Contact Oxford Instrument (I have this system) or Noran Instrument (they
make the similar system) for more information. Your sample should be no
problem (particles on sticky pad).

Hope this helps,

Best regards,

Zhiyu Wang
Sr. Engineer
Maxtor Corp.
Milpitas, CA
(408)432-4421

-----Original Message-----
} From: zhiyu wang [mailto:zhiyuw-at-home.com]
Sent: Monday, November 26, 2001 2:39 PM
To: zhiyu_wang-at-maxtor.com


The problem with this method is that most Bacillus species spores will look
alike by SEM . . . and Bacillus species are very common contaminants. Most
Bacillus species are not pathogenic. Some of the species spores, such as
Bacillus stearothemophilus are used on purpose, like in Spore Strips, which
are put into autoclaves to make sure the proper kill time and temp are
reached. Unless there are some very distinctive characteristics only found
on Bacillus anthracis spores, SEM or any other strictly visual method will
not work to prove the presence of anthrax-causing spores.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112

----- Original Message -----
} From: "Norton, Buddy" {hnorton-at-richmond.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, November 26, 2001 7:35 PM


As discussed by Sara Miller and Tina Schwach, the genus Bacillus can
not be distinguished on the basis of morphology alone. One needs
specific tests: (1) immunological tests such as fluorescent antibody
staining and light microscopy (LM) or even antibody-labeled latex
spheres that would attach to the spores (LM and EM), (2) molecular
amplification techniques where unique sequences are amplified and
identified. Either of these tests take hours rather than days. The
gold standard is still growing the organisms from the spores since
even a single spore is detectable by this method. The downside is
that culturing can take many days.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Tue Nov 27 13:19:51 2001



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 27 Nov 2001 14:07:55 -0500 (EST)
Subject: RE: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mssrs. Wang and Norton,

With this instrumentation, one might be able to detect particles the size
of bacillus spores, but would not be able to tell the difference between
spores of the different bacillus species.

Sara Miller

On Tue, 27 Nov 2001, Wang, Zhiyu wrote:

} Date: Tue, 27 Nov 2001 09:45:59 -0700
} From: Wang, Zhiyu {Zhiyu_Wang-at-Maxtor.com}
} To: "'Norton, Buddy'" {hnorton-at-richmond.edu}
} Cc: "'microscopy-at-MSA.Microscopy.com'" {microscopy-at-sparc5.microscopy.com}
} Subject: RE: detecting Anthrax spores using EM?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, Norton:
}
} There is a SEM/EDS software "gunshot inspection" for particle inspection
} automatically. I use this system for particle analysis on hard drive disk,
} a 95 mm diameter disk with 0.5 um resolution in whole disk scaning. The
} particle size range can be set so that the software ignores the larger one
} or the smaller one (dirt), and locates the particles and does EDS analysis
} automatically. I do not think you need to do EDS, but the image capture and
} classification functions may be good enough for you.
}
} Contact Oxford Instrument (I have this system) or Noran Instrument (they
} make the similar system) for more information. Your sample should be no
} problem (particles on sticky pad).
}
} Hope this helps,
}
} Best regards,
}
} Zhiyu Wang
} Sr. Engineer
} Maxtor Corp.
} Milpitas, CA
} (408)432-4421
}
} -----Original Message-----
} } From: zhiyu wang [mailto:zhiyuw-at-home.com]
} Sent: Monday, November 26, 2001 2:39 PM
} To: zhiyu_wang-at-maxtor.com
} Subject: Fw: detecting Anthrax spores using EM?
}
}
}
} ----- Original Message -----
} } From: "Norton, Buddy" {hnorton-at-richmond.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, November 27, 2001 1:35 AM
} Subject: detecting Anthrax spores using EM?
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Is there a reliable method of detecting Anthrax spores within a very short
} } time, through the use of Electron
} } Microscopy? Do you know anyone working with this method of detection of
} } Anthrax spores? The system I am looking into
} } involves using sticky pads to capture spores in work areas to include
} } heating and cooling vents. The material collected is then
} } taken to an area where a microscope is used to determine the presence or
} } no-presence of the spores. Within a few hours, 2-4
} } hours, there is a determination made if anthrax spores are in fact
} present.
} }
} }
} } $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
} }
} } "Buddy"
} }
} } Howard B. Norton
} } Captain
} } Deputy Chief of Police - Operations
} }
} } Office: 804-289-8723
} } Pager: 804-346-6263
} } Fax: 804-289-8720
} }
} } hnorton-at-richmond.edu
} }
} } 31 UR Drive
} } Special Programs Building
} } University of Richmond Police Department
} } University of Richmond, Virginia 23173
} }
} } For Sale - 1985 Prowler Camper Trailer 32'
} }
} } Those who row the boat seldom have time to rock it.
} }
} } $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
} }
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Tue Nov 27 13:31:36 2001



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Tue, 27 Nov 2001 11:26:32 -0800
Subject: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Endospores are produced by many bacteria in the genera Bacillus and
Clostridium. Many of these bacteria are quite common and most are harmless.
EM could doubtless identify a bacterial endospore as such but could not, by
itself, identify the species as Bacillus anthracis. Even so, a positive
result should elevate your typical "suspicious white poweder" to the status
of a "very credible biohazard threat" and elicit a vigorous response from
the FBI, CDC etc.

Phase contrast light microscopy would be just as capable for this purpose,
and much more convenient than EM. LM and EM would also both provide some
characterization of dispersants mixed with the spores (i.e. bentonite, etc.).

Sample preparation and microscopy should be done with appropriate biosafety
containment measures. Anthrax requires Biosafety II containment with
special attention to elimination of aerosols.

I have designed sampling and inspection procedures for our campus. If you
need further details you can contanct me off list.

At 07:35 PM 11/26/01 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Tue Nov 27 15:02:00 2001



From: J. Cindy Kleier :      j-kleier-at-northwestern.edu
Date: Tue, 27 Nov 2001 14:50:43 -0600
Subject: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi I was wondering if any one has a good electropolish recipe, using a
double jet-polisher to thin a niobium sample, with out using HF if
possible. The sample is needed for high resolution TEM imaging.

Thanks,

Cindy Kleier
EPIC Facility
Northwestern University
j-kleier-at-northwestern.edu



From daemon Tue Nov 27 15:05:43 2001



From: kszaruba1-at-mmm.com
Date: Tue, 27 Nov 2001 15:00:43 -0600
Subject: Re: Whole body fluorescence microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,
In my experiments with skin imaging, the dryer the skin (and flakes) the
brighter the autofluorescence. How would the pups respond to a little
hand lotion? (no experience with this, do lotions autofluoresce?)

As an aside, has anyone else observed that the yellow stratum corneum
autofluorescence (in unfixed skin cross sections) actually increases with
time exposed to the light beam? Is this just localized drying, or
something else (the sections are mounted in 50% glycerol)? Added
fluorescent dyes photobleach as expected.
Karen
--------------------------------------------------------------------------------
Karen S. Zaruba
BioMaterials Technology Center
3M Center Bldg. 270-1S-01
Tel: (651) 737-2971
Fax: (651) 737-5645




Michael Herron {herro001-at-umn.edu}
11/26/01 11:15 AM
Please respond to herro001


To: microscopy-at-sparc5.microscopy.com
cc: (bcc: Karen S. Zaruba/US-Corporate/3M/US)
Subject: Whole body fluorescence microscopy



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


All,

I am using a stereo microscope equipped for epiflourescent GFP
excitation and emission. I am looking at live GFP transgenic mice and
while the technique works quite well, there is a problem with
autoflourescence of the skin surface.

These are mouse pups with little or no hair, and the skin has dander or
flakes that seems to autofluor. I was wondering if anyone might know of
a solution I could quench this external autofluor with? Something mild
would be in order since these are delicate little critters.

Thanks,
Mike

--

________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001-at-umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/






From daemon Tue Nov 27 17:08:47 2001



From: Beauregard :      beaurega-at-westol.com
Date: Tue, 27 Nov 2001 17:53:46 -0500
Subject: Cooke CV 301 Evaporator feed through connection help.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have gotten an old Cooke Vacuum Products CV 301 evaporator to restore but
have no manuals. I received an email 'manual' on how to operate the
vacuum system valves but not how to connect the transformer connections
behind a clear plastic panel to do an evaporation.

There are six wing nuts with connections on a phenolic mounting board
behind a plastic panel and two copper bus bars can be connected about seven
different ways. I believe you connect the bottom right and bottom middle
wing nuts together with one bus bar (the common ground). That connects the
two 6 volt center taps together with the two right hand feed throughs in
the bell jar. Also the top middle nut probably swings to either of the
two bell jar connections in order to do an evaporation. Just guessing.

Volt meter readings could not sort out what the big step down transformer
internal wiring was.

Please email me privately if you know how to connect them without me
shorting the transformer out. The 2 transformer secondary tap SETS are
labeled zero 0, 6 and 12 (volts). I would be glad to call you for your
advice.

Any operating manual concerning the proper connection sequence would be
nice to get in the mail. The manufacturer says he has none for this old
instrument.

Paul Beauregard
Senior Research Associate
PPG Industries
440 College Park Drive
Monroeville, PA 15146



From daemon Tue Nov 27 17:54:30 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 27 Nov 2001 18:49:03 -0500
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron,
Right you are! Oops! Actually, the beam could be even wider if the
distibution wasn't Gaussian. Guess this requires a more expert source
of information.

Ken Converse
owner
Qualtiy Images
third party SEM service
Delta, PA

Ronald Anderson wrote:

} Ken,
}
} You are only seeing the rise part of the beam width distribution curve.
} Wouldn't the distance between the points be the beam radius and not the
} beam diameter?
}
} Ron
}
} Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
} NOTE CHANGES: 1-845-892-2225 Office; -6899 FAX; 1-800-352-4732, pin 8604
} Pager; 1-914-453-2917 Personal Cell Phone
}
}
}



From daemon Tue Nov 27 18:40:42 2001



From: Robert Alain :      Robert.alain-at-inrs-iaf.uquebec.ca
Date: Tue, 27 Nov 2001 18:31:50 -0600
Subject: TEM Conservation of Formvar-carbon coated grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
In summer it is difficult to get nice Formvar-carbon films for electron
microscopy grids. So we prefer to prepare them in autumn or winter,
because the humidity is less important in our laboratory.
My question is, according your experiences, how many times can we
conserve the Formvar-carbon coated grids and at which temperature.

Thank you very much,

Robert Alain
Microscopie Electronique
Inrs-Institut Armand-Frappier
Laval, H7V 1B7
Robert.alain-at-inrs-iaf.uquebec.ca


From daemon Wed Nov 28 00:59:23 2001



From: =?iso-8859-1?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Wed, 28 Nov 2001 07:30:21 +0100
Subject: Re: LM Manual for Quantimet 570

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning for all,


I have full paper technical data for q570, but it is too long to you,
I have this email for q570 "ia-support-at-leica.co.uk", ask people on this
adress (Richard Hayden ?), or find Leica www page and call to him with this
problem,
this q570 is very good image analyser

best regards

Krzysztof Jan Hübner
Foundry Reserach Institute,
ul. Zakopianska 73
Kraków, Poland



From daemon Wed Nov 28 02:57:21 2001



From: Dr. Herbert Schmid :      schmid-at-inm-gmbh.de
Date: Wed, 28 Nov 2001 09:51:57 +0100
Subject: Electropolishing Nb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
Niobium is polished with good results in an acid and water free solution (H
and O atoms are absorbed by unprotected Nb surfaces) using a solution of 10g
Magnesium Perchlorate (Mg[ClO4]2)in 1 liter Methanol (Struers Tenupol: 60 V;
85 mA; temperature -5°C; flow rate 5); ref.: T. Schober and V. Sorajic,
Metallography 6 (1973) 183.
Good luck,
H.K. Schmid




From daemon Wed Nov 28 07:20:12 2001



From: Valdes, Erica R Dr. SBCCOM :      erica.valdes-at-sbccom.apgea.army.mil
Date: Wed, 28 Nov 2001 08:12:14 -0500
Subject: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please, please, please DO NOT take sampling and analysis of suspected
bioattack materials into your own hands. Contact the authorities. If you
are indeed dealing with an agent you have a "hot zone" and have to be in
full protective gear. As someone already mentioned, you have to do the work
at the highest BSL the unknown might contain. Small quantities of
B.anthracis would be BL2 but what if it turns out to be something worse?
You are risking turning your labs into hot zones. Also you are dealing with
a crime scene or a potential crime scene. Anything you do could be
contaminating evidence and jeopardizing the probability of conviction. I
know it is tempting to play detective on your own, but there is an
infrastructure in place for dealing with these things. Anthrax is not the
only agent "out there" and a course of antibiotics isn't going to do the
trick for all of them.

My intention is not to slam Buddy and Mike for their comments, as for all I
know they are part of the infrastructure and are working with appropriate
authorities in what they do. I just don't want anyone to get it into their
heads to go sleuthing into potential incidents with an "it's okay, I'm a
scientist" attitude. Even the people here, who design biodetectors and
train first responders across the country, call in the authorities if they
suspect anything.


Dr. Erica R. Valdes
US Army Edgewood Chemical Biological Center
EC/B Forensic Analytical Center
AMSSB-RRT-CF/Building 3160
Aberdeen Proving Ground, MD 21010

410-436-2608
FAX 410-436-4470
erica.valdes-at-sbccom.apgea.army.mil


-----Original Message-----
} From: Mike Dalbey [mailto:dalbey-at-biology.ucsc.edu]
Sent: Tuesday, November 27, 2001 2:27 PM
To: Microscopy-at-sparc5.microscopy.com


Endospores are produced by many bacteria in the genera Bacillus and
Clostridium. Many of these bacteria are quite common and most are harmless.
EM could doubtless identify a bacterial endospore as such but could not, by
itself, identify the species as Bacillus anthracis. Even so, a positive
result should elevate your typical "suspicious white poweder" to the status
of a "very credible biohazard threat" and elicit a vigorous response from
the FBI, CDC etc.

Phase contrast light microscopy would be just as capable for this purpose,
and much more convenient than EM. LM and EM would also both provide some
characterization of dispersants mixed with the spores (i.e. bentonite,
etc.).

Sample preparation and microscopy should be done with appropriate biosafety
containment measures. Anthrax requires Biosafety II containment with
special attention to elimination of aerosols.

I have designed sampling and inspection procedures for our campus. If you
need further details you can contanct me off list.

At 07:35 PM 11/26/01 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Wed Nov 28 08:33:20 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 28 Nov 2001 09:26:14 -0500
Subject: detecting Anthrax spores using EM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } }
} } } Hi All,
} } }
} } } I have been given a set of blocks of mouse brain (corpus callosum) to
} } } cut. The investigator did her own embedding in Embed-812/Araldite
} } } and the blocks are actually quite nice at first blush. The
} } } semi-thins are gorgeous and I can cut nice light gold-silver
} } } sections, but her boss is interested in the interline structure of
} } } the myelin sheaths, and wants silver-grey sections. The block won't
} } } cut well that thin. I get alternating silver then gold-purple
} } } sections and sections either stick to the knife edge or drag over. I
} } } have tried all the standard tricks: a newly re-sharpened diamond
} } } knife, smallest possible block face, faster cutting speed. I've
} } } played with the meniscus. I even put the blocks back in the oven for
} } } another day. We will get whatever we can out of these blocks (the
} } } result of a many-week treatment), but want to know how she should
} } } vary her formula to get harder blocks next time around. She is using
} } } a pretty standard recipe (Mollenhauer's Mixture No. 1, 1964). It is:
} } }
} } } Embed 812 25 ml
} } } Araldite 15 ml
} } } DDSA 55 ml
} } } DMP-30 1.5%
} } }
} } }
} } } I know that there are other recipes out there, but since I'm a
} } } Spurr's fan, I'm not familiar enough with them to know which ones
} } } will yield what we need. We probably need just a tad more hardness
} } } to enable the really thin sections.
} } }
} } } Thanks in advance,
} } } Lee
} } } --
} } } Leona Cohen-Gould, M.S.
} } } Sr. Staff Associate
} } } Director, Electron Microscopy Core Facility
} } } Manager, Optical Microscopy Core Facility
} } } Joan & Sanford I. Weill Medical College
} } } of Cornell University
} } } voice (212)746-6146
} } } fax (212)746-8175


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Nov 28 08:59:28 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 28 Nov 2001 09:39:01 -0500
Subject: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I looked up two in the Philips Number 5 book that have no HF.

Schrober and Sorajic, 1973, Metallagraphy,6,p.183.
0.05 molar magnesium pechlorate per liter of methanol. 50-70V, -5 deg C.

and another Gidley and Davis, 1967 , J Sci. Instrum. 44, p. 297.
90% methanol
10% perchloric
5.5V, -30C
BE CAREFUL WITH Perchloric acid solutions!!!


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: J. Cindy Kleier [mailto:j-kleier-at-northwestern.edu]
Sent: Tuesday, November 27, 2001 3:51 PM
To: microscopy-at-sparc5.microscopy.com


Hi I was wondering if any one has a good electropolish recipe, using a
double jet-polisher to thin a niobium sample, with out using HF if
possible. The sample is needed for high resolution TEM imaging.

Thanks,

Cindy Kleier
EPIC Facility
Northwestern University
j-kleier-at-northwestern.edu


From daemon Wed Nov 28 09:25:02 2001



From: Judy Trogadis :      trogadisj-at-smh.toronto.on.ca
Date: Wed, 28 Nov 2001 10:18:08 -0500
Subject: fluorescent vascular markers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Microscopists:

We are interested in differentiating between arteries and veins in the adult rat pulmonary vasculature. Are there specific markers or antibodies we could use? A variety of plant lectins exists which label endothelial cells but the literature is confusing about their specificities. They may also preferentially stain only certain organs.

We are perfusing the rats, and cutting thick sections (~100 microns) on a vibratome for confocal microscopy. So staining could be carried out either at the time of perfusion or later, with the sections. Is there enough time for a probe to stick during perfusion? A fluorescent marker is required for the confocal technique.

I look forward to any suggestions.
Thank you

Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph: 416-864-6060 x6337
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca




From daemon Wed Nov 28 10:27:47 2001



From: Melina Meli :      applina-at-libero.it
Date: Thu, 14 Jul 1904 01:42:36 +0000
Subject: Try-Do not reply

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Try if account works



From daemon Wed Nov 28 10:27:47 2001



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 28 Nov 2001 10:20:38 -0600
Subject: Re: TEM Conservation of Formvar-carbon coated grid

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} Hi All,
} In summer it is difficult to get nice Formvar-carbon films for electron
} microscopy grids. So we prefer to prepare them in autumn or winter,
} because the humidity is less important in our laboratory.
} My question is, according your experiences, how many times can we
} conserve the Formvar-carbon coated grids and at which temperature.
}
} Thank you very much,
}
} Robert Alain
} Microscopie Electronique
} Inrs-Institut Armand-Frappier
} Laval, H7V 1B7
} Robert.alain-at-inrs-iaf.uquebec.ca


I have kept C coated formvar grids for negative staining for a couple of years
in an a/c lab. These usually have a medium to "thickish" C coat. I have prepared
these in the summer as well, and eastern KS is fairly humid.
Bruce
Bruce Cutler, Ph.D.
Director, Microscopy & Electronic Imaging Lab
University of Kansas





From daemon Wed Nov 28 10:30:34 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 28 Nov 2001 08:25:58 -0800 (PST)
Subject: Re: fluorescent vascular markers

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Hi Judy,

Just a thought: Could you use a fluorescent marker for endothelial cells
such as CD-31 (PeCam) and then use the autofluorescence of the elastin
sheath that would be around the arteries to distinguish the ateries from
the veins?

Bob Underwood
U of Washington
Seattle

On Wed, 28 Nov 2001, Judy Trogadis wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Fellow Microscopists:
}
} We are interested in differentiating between arteries and veins in the adult rat pulmonary vasculature. Are there specific markers or antibodies we could use? A variety of plant lectins exists which label endothelial cells but the literature is confusing about their specificities. They may also preferentially stain only certain organs.
}
} We are perfusing the rats, and cutting thick sections (~100 microns) on a vibratome for confocal microscopy. So staining could be carried out either at the time of perfusion or later, with the sections. Is there enough time for a probe to stick during perfusion? A fluorescent marker is required for the confocal technique.
}
} I look forward to any suggestions.
} Thank you
}
} Judy
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 8Q
} 30 Bond St.
} Toronto, ON M5B 1W8
} Canada
}
} ph: 416-864-6060 x6337
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
}
}



From daemon Wed Nov 28 12:45:09 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 28 Nov 2001 13:35:57 -0500
Subject: Help for JEOL HITACHI PHILIPS LEO Purchase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,
I would appreciate any PRIVATE feedback I can get from USERS of the
following:

JEOL JEM 1230
Hitachi 7500 or 7600
Philips Technai 12
LEO 910
Quartz Imaging Intranetworking and Image Database Technology
[URL:http://www.quartzimaging.com/index.html]

Thanks for the help to all.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Wed Nov 28 13:40:25 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Wed, 28 Nov 2001 11:27:47 -0800
Subject: Re: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cindy:

We have a paper in our archive of technical reports titled "A Jet
Electropolishing Solution for Silicon Germanium, Tantalum, Niobium, and
Tungsten Rhenium" written by Bernie Kestel. This is for our Model 550
Single Vertical Jet Electropolihser, but it may be useful anyway. I will
put a copy in the mail to you.

You can view other papers and application notes on our website at
www.southbaytech.com.

I hope this helps.

David

"J. Cindy Kleier" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi I was wondering if any one has a good electropolish recipe, using a
} double jet-polisher to thin a niobium sample, with out using HF if
} possible. The sample is needed for high resolution TEM imaging.
}
} Thanks,
}
} Cindy Kleier
} EPIC Facility
} Northwestern University
} j-kleier-at-northwestern.edu

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Wed Nov 28 13:58:52 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 28 Nov 2001 14:47:02 -0500 (EST)
Subject: Re: TEM Conservation of Formvar-carbon coated grid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One possibility would be to make the Formvar films whenever the humidity
permitted it and then coat them later (weeks, months) as needed. For
small particulate particles (e.g., viruses, subcellular organelles)
adherence is better with freshly carbon-coated grids. Alternativily, you
could glow discharge the stored Formvar and carbon coated grids prior to
use to improve spreading and adherence.

Sara Miller

On 28 Nov 2001, Bruce Cutler wrote:

} Date: 28 Nov 2001 10:20:38 -0600
} From: Bruce Cutler {bcutler-at-eureka.idl.ukans.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM Conservation of Formvar-carbon coated grid
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } Hi All,
} } In summer it is difficult to get nice Formvar-carbon films for electron
} } microscopy grids. So we prefer to prepare them in autumn or winter,
} } because the humidity is less important in our laboratory.
} } My question is, according your experiences, how many times can we
} } conserve the Formvar-carbon coated grids and at which temperature.
} }
} } Thank you very much,
} }
} } Robert Alain
} } Microscopie Electronique
} } Inrs-Institut Armand-Frappier
} } Laval, H7V 1B7
} } Robert.alain-at-inrs-iaf.uquebec.ca
}
}
} I have kept C coated formvar grids for negative staining for a couple of years
} in an a/c lab. These usually have a medium to "thickish" C coat. I have prepared
} these in the summer as well, and eastern KS is fairly humid.
} Bruce
} Bruce Cutler, Ph.D.
} Director, Microscopy & Electronic Imaging Lab
} University of Kansas
}
}
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Nov 28 16:19:02 2001



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Wed, 28 Nov 2001 14:11:28 -0800
Subject: LM anthrax spore hazard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree that sampling and analysis of "suspicous white powders" is not a
casual activity and that it must be done with serious consideration of
potential risks. Our activity is fully integrated with existing hazmat
response procedures followed by the campus police and EH&S. This includes
sample collection by EH&S personnel with appropriate personal protection.

Our procedure was created following an incident on campus that the
authorities (i.e. FBI) could not effectively respond to because they were
overwhelmed by such a large number of similar incidents in the aftermath of
the anthrax exposures on the east coast. The FBI agent that did eventually
respond had zero knowledge of microbiology and zero personal protection
gear. I suspect that waiting for a response by someone prepared to deal
with "the highest BSL the unknown might contain" (that would be level IV, I
assume) would mean we would be waiting still, and that a major campus
building would still be closed. (That material proved to be laundry
detergent, by the way.) Based on this experience we concluded that, in
fact, there was NOT an infrastructure in place to deal with these
situations AT THE LEVEL WE ARE CURRENTLY EXPERIENCING THEM. We hope and
expect this will be a very temporary situation.

Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Wed Nov 28 20:54:33 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 28 Nov 2001 21:49:04 -0500
Subject: Digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I am preparing a report comparing digital cameras for TEMs. We are
looking to upgrade our system intending to image negative stains of
heterochromatin samples. At this point we do not have cryo capability. If
any of you have experience with the digital cameras which can be upgraded
for automated tomography, I would appreciate hearing from you.
Rosemary Walsh



From daemon Thu Nov 29 00:05:25 2001



From: zhiyu-wang :      zhiyuw-at-home.com
Date: Wed, 28 Nov 2001 21:56:23 -0000
Subject: Test, delete this email...thanks,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It should work...



From daemon Thu Nov 29 03:51:36 2001



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 29 Nov 2001 03:37:21 -0600
Subject: Re: LM anthrax spore hazard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The incident we had here at Oklahoma State was handled Veterinary medicine
and the State Veterinary Diagnostic lab on campus. The Haz Mat guys showed
up in moon suits but vet med didn't. They came in rubber boots, mask,
coveralls, goggles, gloves and cap the same as they would for a necropsy of
a suspicious animal.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger
}
}
} I agree that sampling and analysis of "suspicous white powders" is not a
} casual activity and that it must be done with serious consideration of
} potential risks. Our activity is fully integrated with existing hazmat
} response procedures followed by the campus police and EH&S. This includes
} sample collection by EH&S personnel with appropriate personal protection.
}
} Our procedure was created following an incident on campus that the
} authorities (i.e. FBI) could not effectively respond to because they were
} overwhelmed by such a large number of similar incidents in the aftermath
of
} the anthrax exposures on the east coast. The FBI agent that did eventually
} respond had zero knowledge of microbiology and zero personal protection
} gear. I suspect that waiting for a response by someone prepared to deal
} with "the highest BSL the unknown might contain" (that would be level IV,
I
} assume) would mean we would be waiting still, and that a major campus
} building would still be closed. (That material proved to be laundry
} detergent, by the way.) Based on this experience we concluded that, in
} fact, there was NOT an infrastructure in place to deal with these
} situations AT THE LEVEL WE ARE CURRENTLY EXPERIENCING THEM. We hope and
} expect this will be a very temporary situation.
}
} Mike Dalbey
}
} Lecturer in Biology
} University of California, Santa Cruz
}
} 831-459-3674
}
} dalbey-at-biology.ucsc.edu
}
}
}



From daemon Thu Nov 29 06:09:13 2001



From: James Young :      durganyoung-at-sympatico.ca
Date: Thu, 29 Nov 2001 07:01:45 -0500
Subject: Antrax Facts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some Anthrax History:



How many anthrax cases have we had in the United States in the last 50
years?



} From January 1955 to December 1999, there were 236 reported cases of
anthrax, most of them cutaneous, in 30 states and the District of Columbia.



When was the last case of inhalational anthrax in the United States?



The last case of inhalational anthrax in the United States, before 2001, was
in 1976 in California. A home craftsman, who worked with yarn, died.
Bacillus anthracis was isolated from some of the imported yarns used by the
patient.



When was the last case of cutaneous anthrax?

The last case of cutaneous anthrax, before 2001, occurred in North Dakota,
in 2000



Jim Young

Those who row the boat seldom have time to rock it.




From daemon Thu Nov 29 08:29:43 2001



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Thu, 29 Nov 2001 10:20:37 -0400
Subject: test message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please ignore this and my previous message, I am testing my e-mail system.

Dave O'Neil


From daemon Thu Nov 29 09:07:03 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Thu, 29 Nov 2001 09:58:23 -0500
Subject: Re: Confocal Imaging of a Vascular Bed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague (Hossler, F, East Tennessee State Med. Sch.) and I have
used a method called corrosion casting to study the rich capillary bed
underlying the trasitional epithelium of the urinary bladder. We used a
modified commercial preparation "MERCOX Blue" for the preparation. The blue
dye is, fortuitously, fluorescent.
In a report given at the recent Philadelphia meeting of MSA (M&A,
Vol6, Suppl 2, pp562-563), Czymmek et al. described the use of confocal
microscopy in an analysis of corrosion casts. They used a Zeiss LSM 510
confocal (exciting the dye with the HeNe(Red) laser (633nm)) and detecting
via a 660nm longpass emission filter. I have repeated this on an Olympus
FV-300 using both the HeNe(R) as above with casts of pig bladder wall
prepared by one of the above authors (Hossler).
I have a particularly useful z-projection of a large, low power,
image stack that I would be willing to send to requestors (69k, jpg) as an
example of what can be accomplished with the application of confocal
microscopy applied to low-power microanatomic studies of fluorescent
objects.
I have recommended the use of Evans Blue (C.I. 23860, CAS #
314-13-6) on several occasions in the past but have seen no results. This
dye is known to form covalent bonds with albumin stoichimetrically and to be
fluorescent in THAT form. It has been used in studies of extravasation
around injury sites as well as for vascular volume studies. There is one
reported incident of complicity in animal carcinogenesis (bolus, i.p. in
rats) mentioned by IPCS INCHEM
(http://www.inchem.org/documents/iarc/iarc/iarc121.htm).

Hope this is of some use.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
CASI Home Page: http://darwin.wcupa.edu/casi/
South Church Street
West Chester, PA, 19383
Office: SS024
Phone: 610-738-0437
FAX: 610-436-3036
eMail: fmonson-at-wcupa.edu
Please call before visiting


From daemon Thu Nov 29 10:07:24 2001



From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 29 Nov 2001 09:57:32 -0600
Subject: Administrivia: No Test Messages !!! Please read the Listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

We are having a sudden rash of test messages. PLEASE review
the FAQ for the Microscopy Listserver. Every one of your
test messages creates over 3000 email messages and needlessly
clogs up the system.

If you need to test your Email program send a private message
to Zaluzec-at-microscopy.com

Nestor
YourFriendly Neighborhood SysOp.





From daemon Thu Nov 29 10:07:36 2001



From: Carol L. Creasey :      creasey-at-cats.ucsc.edu
Date: Thu, 29 Nov 2001 10:00:50 -0600
Subject: Re: Characterization of Colloids

Contents Retrieved from Microscopy Listserver Archives
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} } I am developing a research proposal to investigate organic carbon
} } colloids as a mechanism for contaminant transport of hydrophobic
} } pollutants such as pesticides in surface and ground waters. The
} } colloids generally range in size from 0.001 to 1 micron in size. I
} } would like to characterize these organic colloids. I know that
} } considering the low molecular weight of the carbon that this can be a
} } problem for a lot of the microscopes and and complementary analytical
} } devices. I was wondering if you had any suggestions for how I might
} } characterize these organic colloids?
} }
} } Thank you for your help,
} } Carol Creasey
} } creasey-at-cats.ucsc.edu
}
} --


From daemon Thu Nov 29 13:46:41 2001



From: tbargar-at-unmc.edu
Date: Thu, 29 Nov 2001 13:16:02 -0600
Subject: old EDS Detector

Contents Retrieved from Microscopy Listserver Archives
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I have the chance of receiving a surplus PGT EDS detector. It has not been
used for two years. My question is would the detector's window still be
good? It has been in storage and the detector window has not been kept
cold during these past two years. Any advice is appreciated.

Tom Bargar
tbargar.unmc.edu



From daemon Thu Nov 29 15:49:01 2001



From: Jean Cline :      jcline-at-unlv.edu
Date: Thu, 29 Nov 2001 13:44:54 -0800
Subject: Job Announcement

Contents Retrieved from Microscopy Listserver Archives
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Research Technician

Applications are invited for a full-time, non-tenure track position to
operate a new electron microprobe/SEM facility in the Department of
Geoscience at UNLV. The successful candidate will maintain a user-friendly
facility for faculty, students, and visiting users, establish standard
operating procedures (some to fulfill DOE quality assurance requirements),
and solicit work from outside users. The candidate may conduct research as
time permits; however, the primary focus of the position is to maintain the
facility and to assist UNLV and outside users. The laboratory currently
serves a wide range of research activity in geoscience, biology, chemistry,
engineering, and materials science.
Minimum requirements include a Master's degree in geology, geochemistry, or
an appropriate related field, and electron microprobe and/or SEM
experience. Review of applications will begin immediately and will
continue until the position is filled. Salary will be commensurate with
qualifications and experience. Position is contingent upon funding.

Interested applicants should send a CV, letter of interest detailing your
interest in the position as described above, and names, addresses, phone
numbers, and email addresses of at least three professional references to
Dr. Jean Cline, Department of Geoscience, UNLV, 4505 Maryland Parkway, Box
454010, Las Vegas, NV 89154-4010; phone: 702 895 3262; fax: 702 895 4064,
email: jcline-at-nevada.edu.

For information on the Geoscience department at UNLV visit our Web page at
http://www.unlv.edu. UNLV is an Equal Opportunity/Affirmative Action
employer. Persons are selected on the basis of ability without regard to
race, color, sex, age, national origin, sexual orientation, religion,
disability or veteran status.



Jean S. Cline
Associate Professor, UNLV
Department of Geoscience
4505 Maryland Parkway, Box 454010
Las Vegas, NV 89154-4010

702/895-1091 (phone)
702/895-4064 (fax)
jcline-at-nevada.edu




From daemon Thu Nov 29 16:22:49 2001



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 20 Nov 2001 16:41:48 -0500
Subject: Re: Basic histochemistry

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


"Histochemistry" by Richard Horobin.

"Histological and Histochemical Methods" by John Kiernan

Most (all?) editions of "Cell and Tissue Biology" by Leon Weiss (editor) have a
nice chapter on histochemistry by Helen Padykula.

Grizzi Fabio ICH wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear All
}
} I would like to receive any information about review papers on the basic
} principles of histochemical techniques. I am quite interested to receive
} references of papers on the basic characteristics of a histochemical method
} (reproducibility, specificity etc).
}
} Many thanks.
}
} Fabio Grizzi
} Scientific Direction
} Istituto Clinico HUMANITAS
} Milan, Italy

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Nov 29 16:30:19 2001



From: elir-at-chem.ch.huji.ac.il
Date: Tue, 20 Nov 2001 21:32:28 -0600
Subject: Ask-A-Microscopist: correct setup for a wide illumination in a

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reimar,

It sounds to me very much like a rotifer: these are very common, and can
survive considerable desiccation, and so are often found in roof gutters
(where I remember finding them). I would guess that the "satellite
dish" is the ciliated "wheel" from which the phylum gets its name - I
think that the cilia are either moving too fast or simply too small to
be seen at whatever magnification you are using. This generates a
current, and I remember as a boy watching green spherical algae being
sucked in and macerated.

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+

----- Original Message -----
} From: {reimar_gaertner-at-wsib.on.ca}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, November 21, 2001 3:34 AM


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (elir-at-chem.ch.huji.ac.il) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday,
November 18, 2001 at 13:55:49
---------------------------------------------------------------------------

Email: elir-at-chem.ch.huji.ac.il
Name: Eli rothenberg

Organization: Hebrew University

Education: Graduate College

Location: Jerusalem, Israel

Question: I would like to know what is the correct setup for a wide
illumination in a confocal microscopy arrangement, that is, what
optics should i use in the path of the laser, if i want to get the
maximum illuminated area through my objective,
the setting is as follows: laser-collimated beam-
dichroic mirror-objective-illuminated surface.
(p.s I thought of using a short focusing lens that will focus half
way through the objective so maybe that will give me a wide
illumination on the surface, and/or maybe a diffuser to go along with
it?)

Thanks

Eli

---------------------------------------------------------------------------



From daemon Thu Nov 29 17:07:12 2001



From: Dave Hall :      daveh-at-restechimage.com
Date: Thu, 29 Nov 2001 17:58:11 -0500
Subject: Flatbed scanners

Contents Retrieved from Microscopy Listserver Archives
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I apologize if this message has been posted already. I responded with
this information several days ago and it did not come back to my Inbox
as a message distributed to the List. I wasn't sure if it had made it
out or not.


Ken:

I don't have experience with either of the two scanners you mentioned.
For our customers, we usually recommend Microtek. We have had good
success with the (previously discussed here) ArtixScan 2500 which is
36-bit with a 3.4 dynamic range. Resolution is 2500 dpi (optical) and
the list price is $4,499. (Usually available for less.) (10,000
element CCD)

Microtek also offers (but I have yet to use) the ArtixScan 1100 which is
closer to the ones you mentioned. Optical resolution is 2000 x 1000,
42-bit, and a higher reported dynamic range of 3.9. This one has a list
price of $1,499. (8,000 element CCD)

Both of these use dual plate technology including Microtek's patented
Emulsion Direct Imaging Technology for incredibly sharp transparency
scanning. With E.D.I.T., there is no glass between the scanning lens,
film, or light source, eliminating distortion and Newton rings that
occur with traditional transparency adapters.

I hope this helps. If I can be of any additional assistance, please let
me know.

*Kind Regards,
*Dave Hall
*Resolution Technology, Inc - (614) 921-0045



-----Original Message-----
} From: Ken Bart [mailto:kbart-at-hamilton.edu]
Sent: Monday, November 26, 2001 10:09 AM
To: microscopy-at-sparc5.microscopy.com


Hi:
I am looking to replace my old Epson flat bed scanner and
want a high quality scanner. This instrument is used primarily for
scanning TEM negatives, 35 mm slides, some OCR and needs to be
function in a multi-user environment. I have been considering the
UMAX Powerlook 3000 and the Heidelberg Linoscan 2650 due to their
reported 3.6 dynamic range and 42 bit pixel depth. Does anyone have
experience with these machines? Is there something better?

Thanks!

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/



From daemon Thu Nov 29 17:25:46 2001



From: Shu-You Li :      syli-at-northwestern.edu
Date: Thu, 29 Nov 2001 17:18:04 -0600
Subject: TEM: thickness of holography biprism?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Does anyone know the reasonable thickness of TEM holography biprism? Is 0.5um enough for normal holography?

Thanks,
Shuyou.

______________
Shuyou Li
Dept MSE
Northwestern Univ
2225 N Campus Dr
Evanston, IL 60208

Tel: 1-(847)491-7798(Lab); 1-(847)-869-4348(Home)
Email: syli-at-northwestern.edu; syli16-at-hotmail.com
Web: http://pubweb.northwestern.edu/~sli974


From daemon Thu Nov 29 17:37:03 2001



From: Margie Bryant :      mbryant-at-hsc.usf.edu
Date: Thu, 29 Nov 2001 19:02:21 -0500
Subject: TEM preparations after fluorescence microscopy

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Has anyone successfully processesed tissue (in our case part
of a rat brain) for TEM just after studying brain circulation by
fluorescence microscopy? We use rhodamine and fluorescein tagged AB 40
and the experiment runs for about 1 1/2 hours. At that point, I fix the
tissue with glutaraldehyde and osmium and embed it in epon-araldite.
The problem is that membranes disappear. I'd been warned
that would happen and it did. Is there any way to protect the membranes
or any other stain or method that might help?

Thanks for any help!
Margie Bryant
Anatomy Dept.
Univ. of South Florida Medical School
12901 Bruce D. Downs Blvd
Tampa, Fl. 33612
email address: mbryant-at-com1.med.usf.edu



From daemon Thu Nov 29 18:14:09 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Thu, 29 Nov 2001 16:05:54 -0800
Subject: Philips 410LS for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Consolidation of our EM facilities has resulted in a surplussing a 1984
Philips 410 LS. It has been factory maintained and remains in excellent
condition. The department is asking $30,000, or best offer. For more
information or sales inquiry, please contact:
Dr. Lesnick Westrum
Dept. Neurological Surgery
University of Washington
(206) 543-5434
westrum-at-u.washington.edu

Regards,
Glen
--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Thu Nov 29 18:42:14 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 29 Nov 2001 16:34:43 -0800
Subject: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
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I heard on the radio that the economy is in a recession, so I figured I had
better get busy and try to save money and recycle some supplies. I also had
a big box of used SEM stubs and thought I could start there.

The stubs are left over from various projects, some have silver paint, some
with double stick tape, etc. I thought I could loosen things up a bit by
soaking them in water and/or an Alconox solution. This fit in very well
with my plan for recycling since the plan was to let them soak until the
recession is over.

Since there was no way to know when things would return to normal, I put
some into an ultrasonic cleaner with Fisher Ultrasonic Cleaning solution.
Says it is safe for aluminum and other things.

Now I have a problem. The stubs turned pretty ugly. Big black stains all
over the place. Looks like some reaction between the Al and the Alconox.
Stubs in plain water are not so bad. Ultrasonic cleaner can't remove the
black stuff. I can polish off the black stuff, but that wasn't part of the
plan. This was supposed to be simple.

I don't think the black stuff affects the functioning of the stub, it just
looks ugly and means I have to explain to some users that they are just as
good as the few shiny ones left in the drawer (stubs that is, not users).

So tell me oh mighty metal and materials experts, what is the black stuff,
why did it appear, and is there any easy way to get rid of it? Or is this
some kind of plan to pull the economy back up to speed by making me buy a
bunch of shiny new SEM stubs?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Nov 29 20:25:05 2001



From: David P. Bazett-Jones :      dbjones-at-sickkids.ca
Date: Thu, 29 Nov 2001 21:19:01 -0700
Subject: fluorescence/antibodies

Contents Retrieved from Microscopy Listserver Archives
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I need practical advice for a colleague on conjugating fluorophores to
primary antibodies for immunofluorescence microscopy. She would like to
use Alexa and/or Cyanine labels. Are there particular products/vendors
that people have used with success? What are the problems and pitfalls
to avoid?

Thanks in advance!

Dr. David P. Bazett-Jones
Senior Scientist
Programme in Cell Biology, Research Institute
The Hospital for Sick Children
555 University Avenue
Toronto, ON M5G 1X8
Canada

TEL: 416 813-2181
FAX: 416 813-2235
EMail: david.bazett-jones-at-sickkids.ca




From daemon Thu Nov 29 22:00:19 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 29 Nov 2001 22:48:54 -0500
Subject: Re: old EDS Detector

Contents Retrieved from Microscopy Listserver Archives
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Tom,

You will want to check if the window is OK, but the fact that the detector
has been in storage warm for 2 years does not make it likely that it is
broken.

If I had the chance (and needed the detector, of course!), I would accept
it. I would pump it (for which you will need a pumping adapter) and see if
you get a vacuum (do this *BEFORE* you put liq. N2 in it). You can get by
with a rotary pump vacuum, but if you can get a high vacuum (we have a port
plumbed into one of our evaporaters, with a valve, so we can pump
dtectors), so much the better. If it pumps down, let it pump for a few
hours, fill with nitrogen, then plug the pumping port and remove the
adapter. Let it cool overnight, then fire it up. If you have access to a
Fe55 radioactive source you won't even need to put it on the microscope to
check it out, but I guess that you probably don't have one of those!

I have a pumping adapter for very old Kevex detectors with US threads on
the plug - more recent detectors, and all those from Link have metric
threads. I don't know about PGT, but if yours has US threads, you are
welcome to borrow my adapter - I don't think they vary from manufacturer to
manufacturer.

Good luck!

Tony Garratt-Reed

At 01:16 PM 11/29/2001 -0600, you wrote:
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From daemon Thu Nov 29 22:22:43 2001



From: Larry Allard :      L2A-at-ornl.gov
Date: Thu, 29 Nov 2001 23:16:27 -0500
Subject: Re: TEM: thickness of holography biprism?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shu-You:

Our experience is that 0.3 mm is better. 0.5 mm will work, but the
width of the hologram for a given fringe spacing will be reduced. It
depends on what you mean by "normal holography"...

Larry



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov


From daemon Fri Nov 30 02:36:44 2001



From: Alan Davis :      adavis-at-saipan.com
Date: Fri, 30 Nov 2001 08:27:22 +1000
Subject: cover glass thickness

Contents Retrieved from Microscopy Listserver Archives
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I would ask advise concerning cover glass thickness specs. The objectives
on my microscope are specified for .17 mm thick cover slips. I understand
this is quite important.

I guess the answer must be obvious, but given that the idea mounting
mediun would have the same refractive index as the cover slip, and I
think the oil (in case of an immersion situation), how should one deal
with this? the objects should be in direct contact with the cover slip?

Obviously this is not possible in all cases. Should one such as myself
(unfortunate to have objectives with no correction collar) use even
thinner cover slips to compensate for any distance between the object and
the bottom side of the cover slip?

If I might pose another perhaps equally obvious question, is the reasoning
for use of hanging drop preparations also the need to keep the specimen at
this exact distance?

Then what does water do when doing wet mounts?

Sorry for wasting bandwidth on such ridiculous questions,

Alan Davis

--
Alan E. Davis, Science Instructor
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent." ---Lord Raleigh(aka John William Strutt),or
else
his son, Jr., who was also a scientist.


From daemon Fri Nov 30 03:41:13 2001



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Fri, 30 Nov 2001 04:35:27 -0500
Subject: TEM - Colloidal Gold-Lectin protocols

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopy Listers:

We have a collaborator who is having problems using colloidal gold-lectins
for the post-embedding labeling of protozoa (lots of non-specific binding,
even to Epon and Lowicryl embedding media). Does anyone have any good
protocols for the use of these conjugates?

Thanks in advance,

Rick Powell


*****************************************************************************************
Richard D. Powell * rpowell-at-nanoprobes.com * www.nanoprobes.com
NANOPROBES, Incorporated
95 Horse Block Road, Yaphank, NY 11980-9710, USA

US Toll-free: (877) 447-6266 * Tel: (919) 845-6324 * Fax: (631)
980-3608

Sign up for our newsletter: http://www.nanoprobes.com/Newsletter.html
*****************************************************************************************



From daemon Fri Nov 30 06:47:03 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 30 Nov 2001 06:38:33 -0600
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
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Jon -

I don't know how you could get rid of those ugly stains, but you can
probably get your clients to accept the blackened stubs without a murmur by
saying that you "antiqued" then after seeing Martha Stewart do it :-)

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, November 29, 2001 8:34 PM


I have seen black "smut" on Al alloys etched with alkalis. Although I never
analyzed it, I would guess it was undesolved Al alloying elements (Fe, Cu,
etc). A quick dip in a medium strength nitric acid solution, followed by
water rinse cleaned it right off.

Woody


}
} I heard on the radio that the economy is in a recession, so I
} figured I had
} better get busy and try to save money and recycle some
} supplies. I also had
} a big box of used SEM stubs and thought I could start there.
}
} The stubs are left over from various projects, some have
} silver paint, some
} with double stick tape, etc. I thought I could loosen things
} up a bit by
} soaking them in water and/or an Alconox solution. This fit in
} very well
} with my plan for recycling since the plan was to let them
} soak until the
} recession is over.
}
} Since there was no way to know when things would return to
} normal, I put
} some into an ultrasonic cleaner with Fisher Ultrasonic
} Cleaning solution.
} Says it is safe for aluminum and other things.
}
} Now I have a problem. The stubs turned pretty ugly. Big black
} stains all
} over the place. Looks like some reaction between the Al and
} the Alconox.
} Stubs in plain water are not so bad. Ultrasonic cleaner can't
} remove the
} black stuff. I can polish off the black stuff, but that
} wasn't part of the
} plan. This was supposed to be simple.
}
} I don't think the black stuff affects the functioning of the
} stub, it just
} looks ugly and means I have to explain to some users that
} they are just as
} good as the few shiny ones left in the drawer (stubs that is,
} not users).
}
} So tell me oh mighty metal and materials experts, what is the
} black stuff,
} why did it appear, and is there any easy way to get rid of
} it? Or is this
} some kind of plan to pull the economy back up to speed by
} making me buy a
} bunch of shiny new SEM stubs?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}


From daemon Fri Nov 30 07:21:11 2001



From: John Bonevich :      john.bonevich-at-nist.gov
Date: Fri, 30 Nov 2001 08:16:06 -0500
Subject: Re: TEM: thickness of holography biprism?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those of us with "text-only" email, Larry means 0.3 micrometers.

At 11:16 PM -0500 11/29/01, Larry Allard wrote:
} ------------------------------------------------------------------------
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} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Shu-You:
}
} Our experience is that 0.3 mm is better. 0.5 mm will work, but the
} width of the hologram for a given fringe spacing will be reduced.
} It depends on what you mean by "normal holography"...
}
} Larry
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Nov 30 07:38:27 2001



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Fri, 30 Nov 2001 08:35:21 -0500
Subject: Re: fluorescence/antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used the Alexa kits from Mol Probes to conjugate their visible
fluors to protein-G-purified IgG. I have always gotten satisfactory
results, although my degree-of-labeling was always less than optimal as
reported by Mol Probes. We do almost exclusively laser confocal
microscopy, and I can say that the primaries I made are very useful with
that approach. The visible Alexa fluors are very bright and very resistant
to bleaching. I cannot comment on how they would work in standard
epifluorescence, but my guess is that if one has a decent camera (i.e.,
short exposure times), they should work equally well for this approach.
The only practical pitfall in the conjugation process is separation of
unbound dye from conjugate. Mol Probes includes a simple gravity-run
molecular sizing column in the kit with which to do this. However, if
efficiency of labeling is low, it can be difficult to see the band you want
and, as with any such separation approach, you will definitely experience
some loss of material regardless of how critically you run the column.
Also, if you have never set up a column before, this resin is very "gloply"
and not trivial to pack. So, I thought, "why not dialyze away the unbound
dye?" and Mol Probes says you can do this (they supply the column because
you get faster results and they can justify a higher cost for the kits...).
As I am never in a huge hurry, I tried this. As far as I could see, there
was NO difference in the color intensity of the dialysed material! This
suggests that I had 100% labeling (!). The upside on that particular prep
was that, as Mol Probes also points out, if your immunofluorescence sample
prep is good then you really don't have to worry a lot about unbound dye as
it will be washed out any (only the conjugate should stick). This was the
case for me with that dialyzed prep.

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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396


From daemon Fri Nov 30 08:10:32 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 1 Dec 2001 00:05:02 +1000
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Commercially supplied stubs are anodized, which makes them more resistant to
oxidizing, but with detergent that barrier breaks down. The dark stuff has to
be aluminium oxide, which is much more stable then plain Al. You could argue
that those mounts have character and that the appearance does not matter.

I used to recycle a percentage of mounts, but solvents and detergent are just
too messy. I prefer a fine flat file (could also use sanding paper, a medium to
coarse grade). Peel off any easily removed sticky material and then give the
surface of the mount a few strokes on the file. A double layer of paper towel
under the file collects most of the rubbish and protects the bench.

Its an ok job, if you don't expect to do that full-time for the rest of your
working life. If you are entrepreneurial, leave the set-up with some old mounts
on the bench and hide the new mounts. This is doubly effective if some
influential people come to contemplate your funding problems while cleaning
dirty mounts.

With a bit of tinkering you could have your lab's economy run counter-cyclical
to the one Alan Greenspan is worried about.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, November 30, 2001 10:35 AM, Jon Krupp [SMTP:jmkrupp-at-cats.ucsc.edu]
wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I heard on the radio that the economy is in a recession, so I figured I had
} better get busy and try to save money and recycle some supplies. I also had
} a big box of used SEM stubs and thought I could start there.
}
} The stubs are left over from various projects, some have silver paint, some
} with double stick tape, etc. I thought I could loosen things up a bit by
} soaking them in water and/or an Alconox solution. This fit in very well
} with my plan for recycling since the plan was to let them soak until the
} recession is over.
}
} Since there was no way to know when things would return to normal, I put
} some into an ultrasonic cleaner with Fisher Ultrasonic Cleaning solution.
} Says it is safe for aluminum and other things.
}
} Now I have a problem. The stubs turned pretty ugly. Big black stains all
} over the place. Looks like some reaction between the Al and the Alconox.
} Stubs in plain water are not so bad. Ultrasonic cleaner can't remove the
} black stuff. I can polish off the black stuff, but that wasn't part of the
} plan. This was supposed to be simple.
}
} I don't think the black stuff affects the functioning of the stub, it just
} looks ugly and means I have to explain to some users that they are just as
} good as the few shiny ones left in the drawer (stubs that is, not users).
}
} So tell me oh mighty metal and materials experts, what is the black stuff,
} why did it appear, and is there any easy way to get rid of it? Or is this
} some kind of plan to pull the economy back up to speed by making me buy a
} bunch of shiny new SEM stubs?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}



From daemon Fri Nov 30 08:25:36 2001



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Fri, 30 Nov 2001 08:20:09 -0600
Subject: Cross Sectioning Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been asked to prepare some cross sections of hair for inspection with
an optical microscope and not having much experience doing this I have
gotten some advice locally and tried a few things with an old microtome, but
without much success.

I am wondering if someone on this list could offer me some advice on how to
prepare the hair for the sectioning. So far working with paraffin has not
be successful as the hair seems to not cut well leaving either a hole in the
wax strip or a raised and irregular hair.

Alternately I would be interested in talking to a lab near Connecticut that
could do this sectioning for me.

Thanks very much for any help you may be able to offer!

Richard Shalvoy
Arch Chemicals
Cheshire, CT
203-271-4394
rbshalvoy-at-archchemicals.com


From daemon Fri Nov 30 08:33:34 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 30 Nov 2001 09:29:05 -0500
Subject: Re: fluorescence/antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} I need practical advice for a colleague on conjugating fluorophores to
} primary antibodies for immunofluorescence microscopy. She would like to
} use Alexa and/or Cyanine labels. Are there particular products/vendors
} that people have used with success? What are the problems and pitfalls
} to avoid?
}
} Thanks in advance!
}
} Dr. David P. Bazett-Jones
****************
Dear David,
The Alexa family of dyes are sold by Molecular Probes, and if you buy
them uncongugated, they come with instructions for congugating them
to your Ab. I haven't done it personally, but my coworkers have and
tell me its easy.

I have not financial interest in Molecular Probes, I'm just a
satisfied customer.
Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Fri Nov 30 09:24:37 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 30 Nov 2001 10:15:11 -0500
Subject: Recycling of aluminum mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jonathan Krupp wrote:
=============================================================
I heard on the radio that the economy is in a recession, so I figured I had
better get busy and try to save money and recycle some supplies. I also had
a big box of used SEM stubs and thought I could start there.

The stubs are left over from various projects, some have silver paint, some
with double stick tape, etc. I thought I could loosen things up a bit by
soaking them in water and/or an Alconox solution. This fit in very well with
my plan for recycling since the plan was to let them soak until the
recession is over.

Since there was no way to know when things would return to normal, I put
some into an ultrasonic cleaner with Fisher Ultrasonic Cleaning solution.
Says it is safe for aluminum and other things.

Now I have a problem. The stubs turned pretty ugly. Big black stains all
over the place. Looks like some reaction between the Al and the Alconox.
Stubs in plain water are not so bad. Ultrasonic cleaner can't remove the
black stuff. I can polish off the black stuff, but that wasn't part of the
plan. This was supposed to be simple.

I don't think the black stuff affects the functioning of the stub, it just
looks ugly and means I have to explain to some users that they are just as
good as the few shiny ones left in the drawer (stubs that is, not users).

So tell me oh mighty metal and materials experts, what is the black stuff,
why did it appear, and is there any easy way to get rid of it? Or is this
some kind of plan to pull the economy back up to speed by making me buy a
bunch of shiny new SEM stubs?
===================================================================
In the typical laboratory that "accummulates" no-longer-needed SEM mounts,
the "history" of what was originally there on the mounts (e.g. the samples)
is often times lost with the passing of the months if not also from the
natural turnover of both employees and/or students. We have ourselves
considered offering some kind of a "recycling" program for SEM mounts, but
the inability to really know what we would be getting would pose to our
employees an unacceptable safety risk. Furthermore, from the standpoint of
shipping, some might argue that phenomenologially, "waste" mounts are akin
in lots of ways to a hazardous waste, which would impose restrictions on how
they could be shipped (if indeed they could be shipped easily at all).

Also, some laboratories have a way of accidentally or inadvertently "co-
mingling" or mixing up used beryllium mounts with aluminum mounts, and once
a part of the kind of "mess" that was described, they look no different from
orginary aluminum mounts. Hence you do want to be further cautious when
talking about polishing down or grinding, because you absolutely don't want
to get into a beryllium dust exposure situation, which can surely occur if
the polishing pads or grinding papers dry out.

Disclaimer: SPI Supplies offers new aluminum mounts for all SEMs, and we
would like to see more new mounts being used. But we offer the above
comment not to encourage the purchase of more new mounts, but only to point
out that if you did want to "recycle" used SEM mounts, you do want to be
careful not to mix up beryllium mounts with the aluminum and you also want
to have some awareness of what kinds of samples, might be on the no-longer-
needed mounts.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Fri Nov 30 11:56:47 2001



From: Abizar Lakdawalla :      abizarl-at-innogenex.com
Date: Fri, 30 Nov 2001 09:51:09 -0800
Subject: Re: fluorescence/antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Molecular probes
www.probes.com
have outstanding products.

Abizar
www.innogenex.com

"David P. Bazett-Jones" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I need practical advice for a colleague on conjugating fluorophores to
} primary antibodies for immunofluorescence microscopy. She would like to
} use Alexa and/or Cyanine labels. Are there particular products/vendors
} that people have used with success? What are the problems and pitfalls
} to avoid?
}
} Thanks in advance!
}
} Dr. David P. Bazett-Jones
} Senior Scientist
} Programme in Cell Biology, Research Institute
} The Hospital for Sick Children
} 555 University Avenue
} Toronto, ON M5G 1X8
} Canada
}
} TEL: 416 813-2181
} FAX: 416 813-2235
} EMail: david.bazett-jones-at-sickkids.ca



From daemon Fri Nov 30 11:56:48 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 30 Nov 2001 12:49:24 -0500
Subject: RE: cover glass thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Alan,
First, the mountant's RI is matched to that of the specimen. That
of the immersion oil to that of the glass, although I cheat when I use
Cargille oils of non-standard RI to view some difficult coverglassed
specimens in order to alter the phase dynamics of the system.
Second, the 0.17mm coverglass represents the average thickness of
the No 1 coverglass that one normally uses for the purpose of mounting
specimens for microscopy. Further, the glass components of the objective
have been ground in expectation of that thickness of glass between the
objective and the specimen, whether the objective is immersive or not. Even
though the use of oil immersion to view a blood smear without a coverglass
is common, the oil immersion objective almost always 'says' that it expected
a coverglass to be there.
Third, with respect to specimen thickness, there is nothing that can
change the built-in working parameters of the objectives, thus, you won't be
able to use oil immersion to any good effect on a whole mount of any
specimen much thicker than 10um. A 40x objective is stressed beyond its
limits when one tries to view a whole mounted 24-hr chick embryo. In other
words, the working distance, which is not usually given, defines the limit
of the distance between the face of the objective and the object one wishes
to view. If one places physical impediments between face and specimen that
exceed the working distance, then the specimen cannot be viewed by the
scientist and is crushed by the freshman biology student who doesn't know
about it. On the other side of that is the fact that were the working
distance of the oil immersion lens too long, the oil might not be able to
bridge the gap between objective and coverglass.
Fourth, the information on the barrel of an objective is part of the
description of the optical properties of the objective. Its design
parameter set, if you will. The 0.17 correction tells much about working
distance. The numerical aperture tells a lot about the optical components
used inside the objective.
Fifth, I always thought that the hanging drop was to provide a
method for viewing live material in a confined environmentally friendly
volume (cage).

} ----------
} From: Alan Davis
} Sent: Thursday, November 29, 2001 5:27 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: cover glass thickness
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would ask advise concerning cover glass thickness specs. The objectives
} on my microscope are specified for .17 mm thick cover slips. I understand
} this is quite important.
}
} I guess the answer must be obvious, but given that the idea mounting
} mediun would have the same refractive index as the cover slip, and I
} think the oil (in case of an immersion situation), how should one deal
} with this? the objects should be in direct contact with the cover slip?
}
} Obviously this is not possible in all cases. Should one such as myself
} (unfortunate to have objectives with no correction collar) use even
} thinner cover slips to compensate for any distance between the object and
} the bottom side of the cover slip?
}
} If I might pose another perhaps equally obvious question, is the reasoning
} for use of hanging drop preparations also the need to keep the specimen at
} this exact distance?
}
} Then what does water do when doing wet mounts?
}
} Sorry for wasting bandwidth on such ridiculous questions,
}
} Alan Davis
}
} --
} Alan E. Davis, Science Instructor
} Marianas High School
} PMB 30, Box 10006,
} Saipan, MP 96950
} Northern Mariana Islands
} adavis-at-saipan.com
}
}
} "An inviscid theory of flow renders the screw useless, but the need
} for one non-existent." ---Lord Raleigh(aka John William Strutt),or
} else
} his son, Jr., who was also a scientist.
}
}


From daemon Fri Nov 30 13:17:43 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 30 Nov 2001 13:10:39 -0600
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Commercially supplied stubs are anodized, which makes them
} more resistant to
} oxidizing

It is my understanding that anodized aluminum has much thicker
oxide layer than a natural oxide film, and, as a result,
it should lead to the coloring of the metal (which we do
not see for the stubs). Im I wrong?

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


From daemon Fri Nov 30 13:41:57 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Fri, 30 Nov 2001 14:34:15 -0500
Subject: RE: microtome oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The old AO Spencer rotary microtome manual recommends Pike Oil and a
Neutral grease. I haven't used Pike oil, but I usually purchase a light
machine oil. For use in colder environments such as when used for cryotomy,
this manual recommended a lighter Hamilton watch oil. Clock oil will do.
Others have suggested Singer sewing machine oil. I have used "Bear Oil"
from Norton ("Formerly Pike Oil") which now appears to be Norton Part #
07660 787940 0, 4.5oz "Sharpening Oil" [URL:
http://www.nortonconsumer.com/catalog/displaytier.asp?tier_id=D110050050010&
display=all].

Hope this helps. Getting the proper lubricating oil is always a problem for
these old machines.

P.S.1 I just purchased a new volume of "3 to 1" oil, and was surprised at
its viscosity which I consider to be higher than in previous purchases.

P.S.2 A good rule is that if the oil hole contains a wick, the oil should be
really light! Whatever, the lubricant should NOT be so light that it leaks
out of the bearing.

Regards to all,

Fred Monson

} ----------
} From: Jonathan Wilson
} Reply To: Jonathan Wilson
} Sent: Friday, November 16, 2001 4:51 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: microtome oil
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I have been loaned a microtome (Leitz 1512) by a colleague which
} according to the instruction manual requires regular oiling. The microtome
} did not come with any oil and the price I have been quoted by a local
} supplier for microtome oil was about 75$US (including VAT) for 50ml
} (SAKURA
} 1447). I found the price a bit steep and am left wondering if it is
} absolutely necessary to buy "microtome" oil or if I can use an off the
} shelf
} oil (e.g 3 in 1 or something like that). I do not want to compromise the
} performance of the microtome as it is not mine and would appreciate any
} advice from those with experience.
} Thanks for any help.
} Sincerely,
} Jonathan
}
} Jonathan Wilson (PhD)
} CIIMAR
} Rua do Campo Alegre 823
} 4150-180 Porto, Portugal
} tel: 351 22 608 0470 / 71
} fax: 351 22 606 0423
} e-mail: wilson_jm-at-cimar.org
} web: www.cimar.org
}
}
}


From daemon Fri Nov 30 14:33:27 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 30 Nov 2001 15:25:30 -0500
Subject: Negative Fixing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

I can't put cute subject headings anymore, so I'm trying not to be too negative ;-).

I'm working in a lab where they have been adding a hypo clearing agent called Orbit Bath into the Kodak rapid fix. Has anybody heard of doing this? I have never heard of this and I can't get the Orbit Bath without promising my first born and pulling a few teeth.

I'd rather go back to the tried and true method of developing the Kodak 4489 in HRP or D-19, fixing in Kodak Rapid Fix (using A & B), dunking in Hypo clear and washing as usual.

If someone can come up with a good reason to keep continuing this practice, or even an explanation as to why they even started it, I'm all ears.

Help clear me of my negative thoughts,

Paula :-)


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Fri Nov 30 15:49:47 2001



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 30 Nov 2001 13:41:44 -0600
Subject: RE: cover glass thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One correction to the comment below.

No. 1 coverslips have a range of 0.13 to 0.17 (for Fisher) or 0.13 to
0.16 (for Corning brand).

No. 1 1/2 range from 0.16 to 0.19 for both Corning and Fisher.

Having used a micrometer on both sizes and brands, I find the most
0.17 coverslips in No. 1 1/2 boxes.

Tom



}
}
} Hi Alan,
} First, the mountant's RI is matched to that of the specimen. That
} of the immersion oil to that of the glass, although I cheat when I use
} Cargille oils of non-standard RI to view some difficult coverglassed
} specimens in order to alter the phase dynamics of the system.
} Second, the 0.17mm coverglass represents the average thickness of
} the No 1 coverglass that one normally uses for the purpose of mounting
} specimens for microscopy. Further, the glass components of the objective
} have been ground in expectation of that thickness of glass between the
} objective and the specimen, whether the objective is immersive or not. Even
} though the use of oil immersion to view a blood smear without a coverglass
} is common, the oil immersion objective almost always 'says' that it expected
} a coverglass to be there.
} Third, with respect to specimen thickness, there is nothing that can
} change the built-in working parameters of the objectives, thus, you won't be
} able to use oil immersion to any good effect on a whole mount of any
} specimen much thicker than 10um. A 40x objective is stressed beyond its
} limits when one tries to view a whole mounted 24-hr chick embryo. In other
} words, the working distance, which is not usually given, defines the limit
} of the distance between the face of the objective and the object one wishes
} to view. If one places physical impediments between face and specimen that
} exceed the working distance, then the specimen cannot be viewed by the
} scientist and is crushed by the freshman biology student who doesn't know
} about it. On the other side of that is the fact that were the working
} distance of the oil immersion lens too long, the oil might not be able to
} bridge the gap between objective and coverglass.
} Fourth, the information on the barrel of an objective is part of the
} description of the optical properties of the objective. Its design
} parameter set, if you will. The 0.17 correction tells much about working
} distance. The numerical aperture tells a lot about the optical components
} used inside the objective.
} Fifth, I always thought that the hanging drop was to provide a
} method for viewing live material in a confined environmentally friendly
} volume (cage).
}
} } ----------
} } From: Alan Davis
} } Sent: Thursday, November 29, 2001 5:27 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: cover glass thickness
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I would ask advise concerning cover glass thickness specs. The objectives
} } on my microscope are specified for .17 mm thick cover slips. I understand
} } this is quite important.
} }
} } I guess the answer must be obvious, but given that the idea mounting
} } mediun would have the same refractive index as the cover slip, and I
} } think the oil (in case of an immersion situation), how should one deal
} } with this? the objects should be in direct contact with the cover slip?
} }
} } Obviously this is not possible in all cases. Should one such as myself
} } (unfortunate to have objectives with no correction collar) use even
} } thinner cover slips to compensate for any distance between the object and
} } the bottom side of the cover slip?
} }
} } If I might pose another perhaps equally obvious question, is the reasoning
} } for use of hanging drop preparations also the need to keep the specimen at
} } this exact distance?
} }
} } Then what does water do when doing wet mounts?
} }
} } Sorry for wasting bandwidth on such ridiculous questions,
} }
} } Alan Davis
} }
} } --
} } Alan E. Davis, Science Instructor
} } Marianas High School
} } PMB 30, Box 10006,
} } Saipan, MP 96950
} } Northern Mariana Islands
} } adavis-at-saipan.com
} }
} }
} } "An inviscid theory of flow renders the screw useless, but the need
} } for one non-existent." ---Lord Raleigh(aka John William Strutt),or
} } else
} } his son, Jr., who was also a scientist.
} }
} }

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)


From daemon Fri Nov 30 15:54:24 2001



From: Kai Lorcharoensery :      kai-at-lehigh.edu
Date: Fri, 30 Nov 2001 16:48:28 -0500
Subject: Re: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The thickness of a natural alumina film is in the order of some tens of
nanometers. An anodized layer, on the other hand, can reach the
micrometer level. However, it is porous. Usually, we can fill those
pores simply by immersing parts in boiling or hot water. The film is
nearly transparent or translucent. The color we see is actually the
color of the dye used during pore filling for decoration purpose. There
are many colors we can choose.

Kai Lorcharoensery

Materials Science & Engineering
Lehigh University
Bethlehem, PA

===============Quote==================
"Dusevich, Vladimir" wrote:

It is my understanding that anodized aluminum has much thicker oxide
layer than a natural oxide film, and, as a result, it should lead to the
coloring of the metal (which we do not see for the stubs). Im I wrong?

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy
=========================================


From daemon Fri Nov 30 17:14:46 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Fri, 30 Nov 2001 18:06:45 -0500
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anodizing of aluminum can be done in many colors, as
well as a clear coating. I would not expect SEM stubs to be
anodized, as the aluminum oxide coating which forms is an
insulator. This would inhibit the removal of the electron
charge from the sample. The native oxide film is no where
near the thickness of the usual anodized film, and can easily
be broken. The thickness of the anodized film can be
controlled, but it is still an effective insulator. I am fairly
certain that none of the stubs I have ever used were anodized.

Regards,
Darrell



From daemon Sat Dec 1 03:51:54 2001



From: Gordon Couger :      gcouger-at-provalue.net
Date: Sat, 1 Dec 2001 03:41:43 -0600
Subject: Re: Negative Fixing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Paula Sicurello" {patpxs-at-gwumc.edu}
}
} I'm working in a lab where they have been adding a hypo clearing agent
called Orbit Bath into the Kodak rapid fix. Has anybody heard of doing
this? I have never heard of this and I can't get the Orbit Bath without
promising my first born and pulling a few teeth.
}
} I'd rather go back to the tried and true method of developing the Kodak
4489 in HRP or D-19, fixing in Kodak Rapid Fix (using A & B), dunking in
Hypo clear and washing as usual.
}
} If someone can come up with a good reason to keep continuing this
practice, or even an explanation as to why they even started it, I'm all
ears.
}
Some people use Orbit bath in the fix to cut the time in the fixer. I know
of no advantage to it except it speeds up the process a little but not much.
The main advantage is for fixing fiber paper so the paper spends less time
in the fix and there is less time for fixer to diffuse into the paper.

Gordon

Gordon Couger
Stillwater, OK
www.couger.com/gcouger



From daemon Sat Dec 1 05:20:30 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 1 Dec 2001 21:16:56 +1000
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm certain that every mount sold by ProSciTech is anodized, and by their very
appearance, all other such commercially sold mounts I have seen are anodized
too. True the anodized film is very thin.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, December 01, 2001 9:07 AM, Darrell Miles [SMTP:milesd-at-US.ibm.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Anodizing of aluminum can be done in many colors, as
} well as a clear coating. I would not expect SEM stubs to be
} anodized, as the aluminum oxide coating which forms is an
} insulator. This would inhibit the removal of the electron
} charge from the sample. The native oxide film is no where
} near the thickness of the usual anodized film, and can easily
} be broken. The thickness of the anodized film can be
} controlled, but it is still an effective insulator. I am fairly
} certain that none of the stubs I have ever used were anodized.
}
} Regards,
} Darrell



From daemon Sat Dec 1 07:33:41 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 01 Dec 2001 08:26:43 -0500
Subject: Re: old EDS Detector

Contents Retrieved from Microscopy Listserver Archives
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Tom,
The window should be OK IF:
1.) it was protected from mechanical damage (covered effectively) and

2.) the vacuum in the dewar had not deteriorated much while it was
cold, ie there was still a vacuum after it warmed up.

The question really is: is the SiLi crystal still good? Upon warming
up, it may have gotten contaminated by all of the junk coming out of
the zeolite cry-sorption material.

Basically, many detectors do store fairly well, but I would not invest
much, if any, money in aquiring one unless is was proved to be good. On
the other hand, if you can get it for free, the mounting is correct for
your microscope and you're willing to have it rebuilt, it could be a
good deal. Contact

"Jim Nicolino" {JNicolino-at-xraydetectors.com}

for possible cost. He's done several rebuilds for some of my customers.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA


"tbargar-at-unmc.edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have the chance of receiving a surplus PGT EDS detector. It has not been
} used for two years. My question is would the detector's window still be
} good? It has been in storage and the detector window has not been kept
} cold during these past two years. Any advice is appreciated.
}
} Tom Bargar
} tbargar.unmc.edu
}
}
}
}



From daemon Sat Dec 1 18:31:04 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sun, 2 Dec 2001 10:24:21 +1000
Subject: RE: old EDS Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


True, the most likely defect of a soft-vacuum, stored SiLi detector would be a
contaminated crystal. This "may" be worth fixing in the USA. In Australia, with
the added high freight cost, such detectors become ornaments. At one point I
had three SiLi detectors in use and two attractive, high-technology vases with
dried flower arrangements.
Cheers
Jim Darley
ProSciTech

On Saturday, December 01, 2001 11:27 PM, Ken Converse
[SMTP:qualityimages-at-netrax.net] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Tom,
} The window should be OK IF:
} 1.) it was protected from mechanical damage (covered effectively) and
}
} 2.) the vacuum in the dewar had not deteriorated much while it was
} cold, ie there was still a vacuum after it warmed up.
}
} The question really is: is the SiLi crystal still good? Upon warming
} up, it may have gotten contaminated by all of the junk coming out of
} the zeolite cry-sorption material.
}
} Basically, many detectors do store fairly well, but I would not invest
} much, if any, money in aquiring one unless is was proved to be good. On
} the other hand, if you can get it for free, the mounting is correct for
} your microscope and you're willing to have it rebuilt, it could be a
} good deal. Contact
}
} "Jim Nicolino" {JNicolino-at-xraydetectors.com}
}
} for possible cost. He's done several rebuilds for some of my customers.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
}
} "tbargar-at-unmc.edu"-at-sparc5.microscopy.com wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have the chance of receiving a surplus PGT EDS detector. It has not been
} } used for two years. My question is would the detector's window still be
} } good? It has been in storage and the detector window has not been kept
} } cold during these past two years. Any advice is appreciated.
} }
} } Tom Bargar
} } tbargar.unmc.edu
} }
} }
} }
} }



From daemon Sun Dec 2 17:48:20 2001



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Sun, 2 Dec 2001 17:30:25 -0600
Subject: Re: Cross Sectioning Hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Richard,

Do you need to observe the hair cross-sections in great detail, or are you
just looking at general morphology? If the latter, then you can
cross-section hairs for microscopy by pulling the hairs through a small
hole (0.5 or so mm diameter) in a flat metal sheet (can't remember exactly,
but maybe 1 mm thick - about the same size as a microscope slide). You
will probably need to mix the hairs with cotton so they go through and then
stay tight in the hole - they need to be jammed fairly hard in the hole for
the next part. Then you just cut off the hairs either side with a sharp
razor blade so you get a flat cross-section, mount the metal slide on top
of a regular slide, apply a drop of oil (or preferred mountant) to the
hairs in the hole, coverslip and observe.

This is one way I've seen zoologists identify hairs and characterise
differences.
}
} I have been asked to prepare some cross sections of hair for inspection with
} an optical microscope and not having much experience doing this I have
} gotten some advice locally and tried a few things with an old microtome, but
} without much success.
}
} I am wondering if someone on this list could offer me some advice on how to
} prepare the hair for the sectioning. So far working with paraffin has not
} be successful as the hair seems to not cut well leaving either a hole in the
} wax strip or a raised and irregular hair.
}
} Alternately I would be interested in talking to a lab near Connecticut that
} could do this sectioning for me.
}
} Thanks very much for any help you may be able to offer!
}
} Richard Shalvoy
} Arch Chemicals
} Cheshire, CT
} 203-271-4394
} rbshalvoy-at-archchemicals.com

cheers,
rosemary

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475 or 61-0402 835 973
fax 61-2-6246 5000
email r.white-at-pi.csiro.au


From daemon Sun Dec 2 18:15:58 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Sun, 02 Dec 2001 18:14:08 -0800
Subject: Re: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would certainly hope that SEM stubs are NOT anodized. Not only does anodizing
create an insulating surface, but it also creates a surface with very poor vacuum
properties -- anodized parts will pump down slowly due to the gas trapped in the
porosity.

Fred Schamber
ASPEX, LLC

Jim at Proscitech wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm certain that every mount sold by ProSciTech is anodized, and by their very
} appearance, all other such commercially sold mounts I have seen are anodized
} too. True the anodized film is very thin.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Saturday, December 01, 2001 9:07 AM, Darrell Miles [SMTP:milesd-at-US.ibm.com]
} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Anodizing of aluminum can be done in many colors, as
} } well as a clear coating. I would not expect SEM stubs to be
} } anodized, as the aluminum oxide coating which forms is an
} } insulator. This would inhibit the removal of the electron
} } charge from the sample. The native oxide film is no where
} } near the thickness of the usual anodized film, and can easily
} } be broken. The thickness of the anodized film can be
} } controlled, but it is still an effective insulator. I am fairly
} } certain that none of the stubs I have ever used were anodized.
} }
} } Regards,
} } Darrell



From daemon Sun Dec 2 18:57:17 2001



From: Krzysztof Herman :      kherman-at-labsoft.com.pl
Date: Mon, 3 Dec 2001 01:54:28 +0100
Subject: Fw: EDS detector for EM400

Contents Retrieved from Microscopy Listserver Archives
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Hello senior-TEM-users (Philips...).

I have question:
Is it possible to attach EDS detector to old EM400 WITHOUT goniometer ?
1) What one need to change - polepieces to have a hole ? apertures for low
bkgnd ? or maybe whole goniometer has to be installed ?
2) Does maybe someone have such detector for give/sell away ??

regards

Krzysztof Herman
EO Service
Labsoft, ul.Ba¿ancia 45A
02-892 Warszawa, tel/fx: (+48 22)6449753, 6449750
mobile: (+48 601)307456
www.labsoft.com.pl



From daemon Sun Dec 2 22:00:25 2001



From: Alan Davis :      adavis-at-saipan.com
Date: Mon, 3 Dec 2001 03:50:56 +1000
Subject: Artifacts and blunders

Contents Retrieved from Microscopy Listserver Archives
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I am interested in gaining insight into the role of microscopic artifacts
in the history of biology. May I impose on list members to contribute
particularly glaring examples of misinterpreation of biological facts due
to improper microscopic technique? I apologize if this is off-topic or a
waste of bandwidth.

Let me provide the first example of a misinterpretation and request your
assistance in learning whether this was due to improper use of the
microscope, or perhaps even malfeasance: Sidney Hickson's early work on
Millepora spp. (Cnidaria:Hydrozoa) fire corals. It seems an incredible
lapse, a wholly fabricated natural history account, one that persisted for
a considerable period in the fabric of the mythology of biological
knowledge. I am interested because reproduction of Millepora platyphylla
is the subject of incompleted thesis research of mine.

Hickson published a report on reproduction of "Millepora" around the end
of the 19th Century. (Anong his other errors he synonomyized all species
of Millepora as ecomorphs of one, M. alcicornis.) In this report, which
I do not have available at this time, he included several plates of
drawings depicting a putative sequence of reproductive events in this
organism. We now understand that his depiction is not even close to the
way that Millepora spp. (which were later redesignated as proper
individual species through painstaking work by Boschma---notwithstanding
the issues recently raised by molecular work) reproduce. The depiction
involved dozens of drawings, and a sequence of events based on a
misinterpretation of what are apparently artifacts.

Hickson (of Cambridge University) worked extensively in the field,
including Indonesia and the Philippines. Was his microscopic work done
in the field? Are members of this list enlightened as to Hickson's
methods?

Hickson's erroneous drawings of the medusae of Millepora lived on for over
3/4 of a century in virtually every Invertebrate Zoology textbook
published until the late 1980s or 1990s. His erroneous description of the
medusa of Millepora as lacking a velum led to the designation of a
separate branch of hydromedusae by Mayer, as the only hydrozoan medusa
without a velum. My unpublished observations in the 1980s as well as
published observations by John Lewis of McGill University showed that the
medusae of Millepora spp. clearly possess a velum.

I apologize for monopolizing the bandwidth. I hope this is as fascinating
a topic for others as for myself, and not considered off-topic.

Alan Davis

--
Alan E. Davis, Science Instructor
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.


From daemon Sun Dec 2 23:02:28 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 03 Dec 2001 16:06:28 +1100
Subject: Film Thickness Monitor Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

We wish to set up a film thickness monitor to satisfy the wants of a user
who has to work to ISO 9000 rules. We have a used Varian Auto deposition
system Model 985-7009 which has become separated from its manual and which
has problems.

Does anyone out there have a copy which we could have/get a copy of?

Thanks.
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400
http://srv.emunit.unsw.edu.au/


From daemon Mon Dec 3 04:13:49 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Mon, 3 Dec 2001 20:08:00 +1000
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You are hoping in vain, commercial mounts are anodized, because:
1 The layer is too thin to insulate even at low voltages. SEM have high volts
(even specimen currents are many and not fractions of volts) and low currents.
No problem.
2 Otherwise the mounts would oxidise and I believe that too is not the best
conductor.
3 Al too is porous, if you really care use polished stainless.
4 Does it matter? It would in an ion pumped TEM, but in an SEM?
5 All other suppliers do anodize. That is a better reason than you may think!
6 If we did not, people would buy other supplier's mounts because ours would
look "cheap"

Now, I have a question: How long have you and some other people been using
anodized mounts and not realised that the nice shiny finish is not a polish.
Have you never seen machine finished Al???

If you really want to make a point, place a hundred commercial mounts into the
bottom of the SEM chamber and then repeat that experiment with machine finished
Al mounts. Record vacuua at various points and run the experiment three times.
Report the results. I would be interested to learn if there is any difference.
Those hundred plain Al mounts made in a machine shop would be a trifle
expensive (you could acid strip the anodizing), but cheaper than most
experiments.

Christmas is coming, so lets be charitable: anodized mounts are a good deal and
they are very suitable for SEM work.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, December 03, 2001 12:14 PM, Frederick Schamber
[SMTP:schamber-at-aspexllc.com] wrote:
} I would certainly hope that SEM stubs are NOT anodized. Not only does
} anodizing
} create an insulating surface, but it also creates a surface with very poor
} vacuum
} properties -- anodized parts will pump down slowly due to the gas trapped in
} the
} porosity.
}
} Fred Schamber
} ASPEX, LLC
}
} Jim at Proscitech wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } I'm certain that every mount sold by ProSciTech is anodized, and by their
} } very
} } appearance, all other such commercially sold mounts I have seen are
} } anodized
} } too. True the anodized film is very thin.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Saturday, December 01, 2001 9:07 AM, Darrell Miles
} } [SMTP:milesd-at-US.ibm.com]
} } wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Anodizing of aluminum can be done in many colors, as
} } } well as a clear coating. I would not expect SEM stubs to be
} } } anodized, as the aluminum oxide coating which forms is an
} } } insulator. This would inhibit the removal of the electron
} } } charge from the sample. The native oxide film is no where
} } } near the thickness of the usual anodized film, and can easily
} } } be broken. The thickness of the anodized film can be
} } } controlled, but it is still an effective insulator. I am fairly
} } } certain that none of the stubs I have ever used were anodized.
} } }
} } } Regards,
} } } Darrell


From daemon Mon Dec 3 08:31:41 2001



From: Catherine.Derr-at-gastechnology.org
Date: Mon, 3 Dec 2001 08:20:26 -0600
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I looking at purchasing a microtome for sectioning polymers, primarily
PE. Although I am familiar with microtomes used in biological studies, I
have
no experience in sectioning polymers. Has anyone sectioned polymers with
repeated success? What features are desired for sectioning polymers? Is
there a specific knife angle that works well?

KD Derr
SEM/Polymer Technician
Gas Technology Institute
1700 S Mount Prospect Rd
Des Plaines, IL 60018
(847) 768-0505 (phone)
(847) 768-0569 (fax)
kd.derr-at-gastechnology.org





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From daemon Mon Dec 3 08:33:18 2001



From: Ann-Fook Yang :      yanga-at-EM.AGR.CA
Date: Mon, 03 Dec 2001 09:30:57 -0500
Subject: Re: Negative Fixing

Contents Retrieved from Microscopy Listserver Archives
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Hi Paula,

I have never used Orbit bath nor Hypo. In fact the former is totally new to me. I have heard of Hypo in the '60s. I was curious and went to the expert.
I phoned Kodak Scientific Support (800-225-5352) to find out whether developing the 4489 film in D19 and fixing in Kodak Rapid Fixer is adequate. The answer is "Yes"
What about Hypo? He asked, "Who makes it?"
He assured me that nothing else is needed after Rapid Fix. Just wash the negs. in water will do.





AnnFook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Room 2091, Bldg. 20,
Central Experimental Farm,
Ottawa, Ontario
Canada K1A 0C6

Tel: 1-613-759-1638
Fax: 1-613-759-1701

e-mail: yanga-at-em.agr.ca

} } } "Paula Sicurello" {patpxs-at-gwumc.edu} 11/30/01 03:25PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Listers,

I can't put cute subject headings anymore, so I'm trying not to be too negative ;-).

I'm working in a lab where they have been adding a hypo clearing agent called Orbit Bath into the Kodak rapid fix. Has anybody heard of doing this? I have never heard of this and I can't get the Orbit Bath without promising my first born and pulling a few teeth.

I'd rather go back to the tried and true method of developing the Kodak 4489 in HRP or D-19, fixing in Kodak Rapid Fix (using A & B), dunking in Hypo clear and washing as usual.

If someone can come up with a good reason to keep continuing this practice, or even an explanation as to why they even started it, I'm all ears.

Help clear me of my negative thoughts,

Paula :-)


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax





From daemon Mon Dec 3 08:40:40 2001



From: zaluzec-at-microscopy.com
Date: Mon, 3 Dec 2001 08:31:45 -0600
Subject: Al SEM Stubs: is it Anodized or Not....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



As I recall from my metallurgy

To "Anodize" is to subject a metal to an electrolytic action as the
anode of a cell in order to coat/deposit a protective or decorative film.

Most aluminium anodization that I have seen creates a dark
THICK oxide film (tens to hundreds of nm thick) and is insulating.

I've never seen this on an aluminium SEM stub. Certainly many I've used are
electropolished and some (the ones we make here in our machine shop )
are simply milled and mechanically polished. All will at a minimum
have a native oxide.
All are conductive, and yes (Jim) I've seen polished
(electrochemical, mechanical) as
well as all ranges of "machine" finished products. These are NOT anodized, they
are all simply oxidized by leaving them sit in air. The native oxide
which forms
is a few tens of angstroms thick and is stable, by definition this is not
anodization. I have never heard of anyone calling this process "anodization"
should that be what you are referring to.

So... now I'm curious and I would request that the various suppliers
who like reply
to this list to fill us all in on what you do.

1.) How many of you anodize your stubs (ie. coat), vs electropolish,
vs mechanically polish.
2.) If you intentionally anodize, (ie. deposit/coat) your stub with
an electrodeposited layer
how thick is your film, and what do you anodize it with.
3.) It might also help to define the "anodization" process (if you do
this) so that we are all
understand what each of you mean in the context of how you make the
stubs we purchase.

Nestor
Your Friendly Neighborhood SysOp.



From daemon Mon Dec 3 08:41:44 2001



From: gary.m.brown-at-exxonmobil.com
Date: Mon, 3 Dec 2001 08:34:55 -0600
Subject: RE: Cleaning SEM stubs

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Jim,

Orbit bath is very useful. It should not, however, be added to the fixer.
The purpose of the hypo clearing agent is to remove the residue of fixer
from the negatives after fixing. I strongly recommend that Orbit not be
added to the fixing bath.

Good luck,

Gary M. Brown




Jim at
Proscitech To: "'Darrell Miles'" {milesd-at-US.ibm.com} ,
{jim-at-proscitech "Microscopy-at-sparc5.microscopy.com"
.com} {Microscopy-at-sparc5.microscopy.com}
cc:
Subject: RE: Cleaning SEM stubs
12/01/01 05:16
AM
Please respond
to
"jim-at-proscitech
.com"





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm certain that every mount sold by ProSciTech is anodized, and by their
very
appearance, all other such commercially sold mounts I have seen are
anodized
too. True the anodized film is very thin.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, December 01, 2001 9:07 AM, Darrell Miles
[SMTP:milesd-at-US.ibm.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Anodizing of aluminum can be done in many colors, as
} well as a clear coating. I would not expect SEM stubs to be
} anodized, as the aluminum oxide coating which forms is an
} insulator. This would inhibit the removal of the electron
} charge from the sample. The native oxide film is no where
} near the thickness of the usual anodized film, and can easily
} be broken. The thickness of the anodized film can be
} controlled, but it is still an effective insulator. I am fairly
} certain that none of the stubs I have ever used were anodized.
}
} Regards,
} Darrell








From daemon Mon Dec 3 08:54:36 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 3 Dec 2001 08:46:17 -0600
Subject: Negative Fixing

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Hi Paula,

Orbit Bath was, as you say, a hypo-clearing agent designed to decrease water
wash times while increasing removal of residual fixer from photographic
materials after processing. I believe it is no longer made. We recently
sent a BUNCH of this stuff to our chemical recycling folks here on campus,
because we do so little silver-based photographic work anymore. We retained
a small stock.

Although also used for films, Orbit and similar chemicals had their heydey
before the routine use of resin-coated papers, which don't absorb chemicals
readily. The fiber-based materials used commonly in the past were (and are)
very elegant papers and for my money are still the best photographic papers
made. However, for anything other than fine-arts use, resin coated papers
have rightfully taken over, with the advantages of decreased processing
times and chemical consumption.

By way of interesting trivia, I had once heard that some of the
fixer-removal products got their start after someone discovered that washing
photo materials in seawater after processing (maybe because it was the only
water they had available in an emergency) was more efficient than fresh
water. Does anyone know if this has a basis in fact or is it just urban
myth?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





-----Original Message-----
} From: Paula Sicurello [mailto:patpxs-at-gwumc.edu]
Sent: Friday, November 30, 2001 2:26 PM
To: microscopy-at-sparc5.microscopy.com


Hi Listers,

I can't put cute subject headings anymore, so I'm trying not to be too
negative ;-).

I'm working in a lab where they have been adding a hypo clearing agent
called Orbit Bath into the Kodak rapid fix. Has anybody heard of doing
this? I have never heard of this and I can't get the Orbit Bath without
promising my first born and pulling a few teeth.

I'd rather go back to the tried and true method of developing the Kodak 4489
in HRP or D-19, fixing in Kodak Rapid Fix (using A & B), dunking in Hypo
clear and washing as usual.

If someone can come up with a good reason to keep continuing this practice,
or even an explanation as to why they even started it, I'm all ears.

Help clear me of my negative thoughts,

Paula :-)


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Mon Dec 3 10:15:46 2001



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Mon, 3 Dec 2001 10:07:29 -0600
Subject: TEM Magnification Calibration

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We have been using carbon grating replicas for calibrating the magnification
of our TEM images for many years. We are in the process of revising our imaging
and morphometry protocols. I am not happy with the precision and longevity of
the carbon grating replicas. Are there more precise and/or stronger standards
for TEM magnification calibration? The final magnifications of our images range
from 3,000 -20,000 X.

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-umn.edu



From daemon Mon Dec 3 10:17:17 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 03 Dec 2001 11:12:38 -0500
Subject: "Anodized" aluminum mounts

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Fred Schamber wrote:
===============================================================
I would certainly hope that SEM stubs are NOT anodized. Not only does
anodizing create an insulating surface, but it also creates a surface with
very poor vacuum properties -- anodized parts will pump down slowly due to
the gas trapped in the porosity.
================================================================
Fred is certainly correct.

SPI Supplies has offered "lathe finish" aluminum SEM mounts for nearly
thirty years and while we have offered different types of surface finishes,
none have ever involved the use of an anodization process. I think the same
would be true for the other major manufactures of SEM mounts but I would not
want to speak for them since I really don't know exactly what they might or
might not have offered over the years. To apply a nonconductive layer to
an SEM mount would surely, to me at least, seem quite counter productive.

I think that there is a problem with semantics here. Let me quote from my
trusty Webster's New Word Dictionary:

-----------------------
an•o•dize
to put a protective, often colored, oxide film on (a light metal) by an
electrolytic process in which the metal serves as an anode.
----------------------

I was happy to see that my memory was not failing me.

The nornal, naturally occurring oxide layer that forms on bare exposed
aluminum is just that: an oxide layer. It is very thin, and generally not
enough to cause anyone real complications with charging. If this is the
layer people are talking about, then so far as I know, no one (well almost
no one) would consider this to be an "anodized" layer. After all, we are
talking about a layer intentionally applied, not one that forms naturally
upon exposure to air.

I can not comment about what some other firm(s) might be doing, but if they
are indeed anodizing their aluminum mounts, they would be growing a nice
oxide layer, much thicker than the naturally occurring kind, and one that
certainly would exhibit lower conductivity characteristics.

SPI Supplies and to the best of my knowledge, the other leading firms
offering SEM mounts, offer aluminum (often times an alloy rather than pure
aluminum) that is either "lathe finish", or "polished" or an intermediate
finish, called by SPI Supplies as its Luster™ finish. But in no case is an
oxide layer being intentionally grown onto the aluminum surface using any
kind of anodization process. Details about the SEM mounts produced by SPI
Supplies can be found on the SPI Supplies website given below.

Actually, what is involved here is a bit more than just semantics. The
naturally formed oxide layer is many times thinner than an anodized layer,
and if my memory is right, that naturally occurring layer is on the order of
5 nm or less. Most anodized layers we have seen over the years (by cross-
section TEM, for example) are on the order of 500 - 1000 nm (or more), and
all exhibit high porosity (and therefore surface area). Until now, I have
not been aware that anyone would call the naturally formed oxide layer to be
an anodized layer, but perhaps I am not keeping up with the more modern use
of the term. Someone help me out if I am wrong about this.

Chuck


===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Mon Dec 3 11:28:25 2001



From: Frederick Schamber :      schamber-at-aspexllc.com
Date: Mon, 03 Dec 2001 11:24:17 -0800
Subject: Re: Cleaning SEM stubs

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Jim,

I will be the first to admit that I am not an expert on either SEM stubs or anodizing,
but I have had very negative experience with the latter in vacuum systems. I once
designed some relatively small black-anodized parts into a SEM and spent considerable
time trying to find out why we had a degradation in pumping speed -- finally, someone
wiser than me sanded off the anodizing and the vacuum was fine. So I have, in effect,
done the experiment you describe -- ordinary machined aluminum parts are vastly
superior to anodized aluminum parts in terms of their vacuum properties in my
experience (and I did some followup experiments in a test system employed specifically
to measure vacuum properties of materials and confirmed that a little bit of anodizing
is a surprisingly bad thing). If one actually did the experiment you propose with one
hundred anodized stubs, my bet is that you would be shocked (but in order to do the
experiment, I think you would first have to have MOST stubs anodized). It is not a
question of the mere "roughness" of the surface.Apparently the anodizing process
creates very tiny interstices which hold the dye, and this is what causes the problem
-- or so I have assumed. I suppose it could be the dye itself -- I never tried the
experiment on "clear anodized" parts.

When I look at catalog advertisements for aluminum SEM stubs, I always see reference to
various degress of polishing, and don't recall ever seeing reference to anodizing.
Reading the description, it certainly sounds like they are just pure uncoated aluminum
-- I've never looked at the ones you sell.

} From an electrical standpoint, I also really wonder how anodizing could work. I know
that anodizing must be avoided on electrical cabinetry because one cannot establish
reliable contacts (a different surface treatment process is used on aluminum instead).
I'm sceptical about the "thin coating" concept since it is my understanding that it is
precisely the insulating layer which protects the surface from oxidation. Any amount
of insulation on a SEM stub would be a big problem. (Of course, the same thing can be
said about the thin oxide layer that naturally forms when aluminum is exposed to air.)
But it is my understanding that anodizing is a process which modifies the surface, not
a simple "coating" which can be "thin".

My negative opinions about anodizing for vacuum systems aren't just mine -- in fact, I
thought they were "common knowledge". For example, I refer you to the very informative
vacuum information site maintained by Roy Schmaus at the University of Alberta
http://nyquist.ee.ualberta.ca/~schmaus/vacf/index.html
Under Basics/An Introduction to Materials for use in Vacuum we find the following
statement: "Aluminum that will be exposed to vacuum should never be anodized due to
serious outgassing problems." Under "References" on the same page he notes: "I
recently had a call from someone who has used Anodized Aluminum as an INSULATOR down
into the ultra high vacuum region. Admittedly the pieces used were very small and
INITIAL OUTGASSING WAS A PROBLEM but low costs were a major advantage." [my EMPHASIS
added]

So given my own negative experience and the congruent remarks of others who work in
this field, I have long understood that anodizing was something to always avoid in
vacuum systems -- and I've ASSUMED that applied to SEM stubs. Am I wrong?

How DO polished stubs stay so shiny? I've wondered the same. However, anodized parts
I have seen always have a kind of "matte" appearance, rather than a high gloss in any
case.

So, though it is entirely possible that I will learn something from this discussion
that I did not know previously (it would hardly be the first time), my limited grasp of
the subject still convinces me that SEM stubs should NOT be anodized. But how do they
retain their surface finish and conductivity? Your response suggests that you
manufacture SEM stubs and call out an anodized surface -- did I read this correctly?
Or are you purchasing stubs which you assume are anodized? I really would be
interested if someone who is in the business of manufacturing these things could
comment directly to this -- without disclosing proprietary information of course.

"Charity" in view of the season notwithstanding, I consider this a matter of
considerable practical importance. If I am in error about this, I want to know it,
since a non-insulating, non-outgassing, but still protective anodizing surface would be
a very good thing. If I am correct, then others reading this exchange should know that
anodizing is something that should be scrupulously avoided in vacuum systems (SEM and
otherwise). This is the kind of thing where a little misinformation can cause a great
deal of grief, so getting to the bottom of this will presumably be a good thing for
everyone. I'm prepared to learn from new facts, if someone can put them on the table.

Fred Schamber
ASPEX, LLC

Jim at Proscitech wrote:

} You are hoping in vain, commercial mounts are anodized, because:
} 1 The layer is too thin to insulate even at low voltages. SEM have high volts
} (even specimen currents are many and not fractions of volts) and low currents.
} No problem.
} 2 Otherwise the mounts would oxidise and I believe that too is not the best
} conductor.
} 3 Al too is porous, if you really care use polished stainless.
} 4 Does it matter? It would in an ion pumped TEM, but in an SEM?
} 5 All other suppliers do anodize. That is a better reason than you may think!
} 6 If we did not, people would buy other supplier's mounts because ours would
} look "cheap"
}
} Now, I have a question: How long have you and some other people been using
} anodized mounts and not realised that the nice shiny finish is not a polish.
} Have you never seen machine finished Al???
}
} If you really want to make a point, place a hundred commercial mounts into the
} bottom of the SEM chamber and then repeat that experiment with machine finished
} Al mounts. Record vacuua at various points and run the experiment three times.
} Report the results. I would be interested to learn if there is any difference.
} Those hundred plain Al mounts made in a machine shop would be a trifle
} expensive (you could acid strip the anodizing), but cheaper than most
} experiments.
}
} Christmas is coming, so lets be charitable: anodized mounts are a good deal and
} they are very suitable for SEM work.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Monday, December 03, 2001 12:14 PM, Frederick Schamber
} [SMTP:schamber-at-aspexllc.com] wrote:
} } I would certainly hope that SEM stubs are NOT anodized. Not only does
} } anodizing
} } create an insulating surface, but it also creates a surface with very poor
} } vacuum
} } properties -- anodized parts will pump down slowly due to the gas trapped in
} } the
} } porosity.
} }
} } Fred Schamber
} } ASPEX, LLC
} }
} } Jim at Proscitech wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } I'm certain that every mount sold by ProSciTech is anodized, and by their
} } } very
} } } appearance, all other such commercially sold mounts I have seen are
} } } anodized
} } } too. True the anodized film is very thin.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } ABN: 99 724 136 560 www.proscitech.com
} } }
} } } On Saturday, December 01, 2001 9:07 AM, Darrell Miles
} } } [SMTP:milesd-at-US.ibm.com]
} } } wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Anodizing of aluminum can be done in many colors, as
} } } } well as a clear coating. I would not expect SEM stubs to be
} } } } anodized, as the aluminum oxide coating which forms is an
} } } } insulator. This would inhibit the removal of the electron
} } } } charge from the sample. The native oxide film is no where
} } } } near the thickness of the usual anodized film, and can easily
} } } } be broken. The thickness of the anodized film can be
} } } } controlled, but it is still an effective insulator. I am fairly
} } } } certain that none of the stubs I have ever used were anodized.
} } } }
} } } } Regards,
} } } } Darrell



From daemon Mon Dec 3 11:34:55 2001



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Mon, 03 Dec 2001 09:29:42 -0800
Subject: artifacts and blunders

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Surely the most famous misinterpretations of microscopical observations are
those recorded in drawings made by preformationists in the 18th Century of
the "homunculus" in the head of human sperm. Several of these drawings are
commonly reprinted in textbooks as cautionary examples.

For a discussion of a common histological artifact see "Multinucleate Plant
Cells" by Burkholder and Mc Veigh (1941) in vol. 68 of the Bulletin of the
Torrey Botanical Club p. 395.

You should also check out what the web has to offer on the history of Royal
Rife and the Rife Microscope.

Finally, evidence seems to be accumulating that the "nanobacteria" observed
by EM in the martian meteorite are artifacts.

Mike Dalbey

Lecturer in Biology
University of California, Santa Cruz

831-459-3674

dalbey-at-biology.ucsc.edu



From daemon Mon Dec 3 12:26:50 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 03 Dec 2001 13:18:44 -0500
Subject: NESM Fall Symposium

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Some subscribers to the list did not see this message the first time I
posted it. At Nestor's suggestion, I am re-posting it, with apologies to
those who did see it the first time!

Tony G-R

-------------

The New England Society for Microscopy (NESM) will be holding it's 35th
Annual Fall Symposium at UMASS-Lowell in Lowell, Massachusetts on Friday,
Dec. 7, 2001.

The meeting will begin at 12 Noon with Registration in the Mil Conference
Center-Wannalancit Building (North Campus).

There will be 3 Sessions: A special student session beginning at 1:05 pm
with 4 presentations, followed by a Poster session and coffee break.

Session II (Biological) begins at 2:20 pm and has as it's speaker, Ken
Moore (University of Iowa), the National MSA Speaker. His talk will
be: "Application of Microscopy Techniques to the Study of Genetic Therapy
Research".

Another Poster Session and Afternoon Coffee Break will follow at 3:20 pm.

Session III (Materials Science) will begin at 3:40 pm and have 2
speakers: Elen Humphreys from M.I.T. and Koichi Nishikida from Thermo
Spectra-Tech.

The Annual Business Meeting will convene at 5:00 pm and will include the
election of NESM officers for 2002.

A Dinner and after-dinner speaker will follow at 6 pm.

Advance registration is due by Friday, November 30th. Registration after
November 30th will NOT include dinner. The registration for NESM members
is $10.00 and $25.00 for non-members. Dinner is $15.00 extra.

For more detailed information, please contact Mary McCann, NESM Treasurer
at mccanns-at-tiac.net or NESM's
website: http://www.msa.microscopy.com/MSALAS/NESM/NESMHome.htm






** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**




From daemon Mon Dec 3 12:49:53 2001



From: Griffiths, Vern :      VGriffiths-at-mtech.edu
Date: Mon, 3 Dec 2001 11:41:35 -0700
Subject: RE: Cleaning SEM stubs

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----------
From: Griffiths, Vern
Sent: Friday, November 30, 2001 11:34 AM
To: 'microscopy-at-sparc5.microscopy.com'
Subject: RE: Cleaning SEM stubs

We have a metallographic lab next door. I've cleaned and reused
hundreds of stubs. After I've accumulated 50 - 100 stubs, I put them in an
ultrasonic cleaner in acetone for five to ten minutes. This gets off most
carbon paint and adhering specimen stuff and the like. Then I take the
stubs, one at a time, and polish/grind them on a 320 grit silicon carbide
paper on a standard metallographic polishing wheel. I generally do the
sides and one end, sometimes (rarely) both ends and they look like new. A
relatively negligible amount of material is removed so that I have stubs on
which this operation has been performed many times. Each stub may require
30 - 60 seconds total so that you may find this time consuming but for us
it's worth the effort. I don't know what the black stuff you mention is,
but aluminum will corrode in aqueous solutions and the Alconox is probably
alkaline enough to aggravate the situation.
Vern Griffiths, Montana Tech
----------
From: jmkrupp-at-cats.ucsc.edu {mailto:jmkrupp-at-cats.ucsc.edu}
[SMTP:jmkrupp-at-cats.ucsc.edu] {mailto:[SMTP:jmkrupp-at-cats.ucsc.edu]}
Sent: Thursday, November 29, 2001 5:35 PM
To: Microscopy-at-sparc5.microscopy.com
{mailto:Microscopy-at-sparc5.microscopy.com}
Subject: Cleaning SEM stubs
-------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
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-----------------------------------------------------------------------.


I heard on the radio that the economy is in a recession, so
I figured I had
better get busy and try to save money and recycle some
supplies. I also had
a big box of used SEM stubs and thought I could start there.

The stubs are left over from various projects, some have
silver paint, some
with double stick tape, etc. I thought I could loosen things
up a bit by
soaking them in water and/or an Alconox solution. This fit
in very well
with my plan for recycling since the plan was to let them
soak until the
recession is over.

Since there was no way to know when things would return to
normal, I put
some into an ultrasonic cleaner with Fisher Ultrasonic
Cleaning solution.
Says it is safe for aluminum and other things.

Now I have a problem. The stubs turned pretty ugly. Big
black stains all
over the place. Looks like some reaction between the Al and
the Alconox.
Stubs in plain water are not so bad. Ultrasonic cleaner
can't remove the
black stuff. I can polish off the black stuff, but that
wasn't part of the
plan. This was supposed to be simple.

I don't think the black stuff affects the functioning of the
stub, it just
looks ugly and means I have to explain to some users that
they are just as
good as the few shiny ones left in the drawer (stubs that
is, not users).

So tell me oh mighty metal and materials experts, what is
the black stuff,
why did it appear, and is there any easy way to get rid of
it? Or is this
some kind of plan to pull the economy back up to speed by
making me buy a
bunch of shiny new SEM stubs?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu {mailto:jmkrupp-at-cats.ucsc.edu}




From daemon Mon Dec 3 12:56:44 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 03 Dec 2001 13:52:38 -0500
Subject: TEM Calibration

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John M. Basgen wrote:
===============================================================
We have been using carbon grating replicas for calibrating the magnification
of our TEM images for many years. We are in the process of revising our
imaging and morphometry protocols. I am not happy with the precision and
longevity of the carbon grating replicas. Are there more precise and/or
stronger standards for TEM magnification calibration? The final
magnifications of our images range from 3,000 -20,000 X.
================================================================
Have you seen the MAG*I*CAL™ TEM Calibration Specimen as described on the
SPI website, page URL
http://www.2spi.com/catalog/standards/magical.html

It seems to get around most of the problems associated with the carbon
grating replicas and although nothing is forever, it is quite robust in
comparison and should last quite a long time.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


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From daemon Mon Dec 3 13:14:44 2001



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Mon, 3 Dec 2001 14:09:18 -0500
Subject: RE: Cleaning SEM stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim,

As far as the finish goes, I have machined aluminum myself, and
have left a far "shinier", superior finish on the aluminum, compared
to what the normal SEM stubs show. I must admit that I have never
had any from ProSciTech, but from your statement, they are very
similar. It must be more than the finish that makes you spend the
money to have them anodized.

True, the freshly machined aluminum forms a native oxide fairly fast,
but it is nowhere near the thickness of the oxide formed by anodizing.

I'm not trying to be uncharitable, I am just surprised that someone
would go to the expense of anodizing SEM stubs, which also forms
an insulating film. I have made my own mounts for specific uses
which are not anodized, and they don't look any different from the
commercially acquired ones, even after years of use. Of course, the
proper alloy needs to be used, or you WILL have vacuum problems.

Have a happy Holiday...
Darrell



From daemon Mon Dec 3 13:44:45 2001



From: Paula Allan-Wojtas :      AllanWojtasP-at-EM.AGR.CA
Date: Mon, 03 Dec 2001 14:19:59 -0500
Subject: Food Structure and Functionality Symposium 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Food Structure & Functionality Forum Symposium 2002
May 5 to 8, 2002, Palais des Congres de Montreal, Montreal, Quebec, Canada

An international symposium leading Food Structure & Functionality studies through the 21st century

"webaddress at the AOCS site (http://www.aocs.org/member/division/fsff/index.htm)",
bulletin board (http://www.aocs.org/ubbcgi/ultimatebb.cgi

held in conjunction with the 93nd AOCS Annual Meeting & Expo (www.aocs.org)

The mandate of the Food Structure & Functionality Forum : "To promote global
collaboration between Food and Agriculture professionals in Structure and Functionality
disciplines by facilitating and providing a forum for exchange of knowledge, expertise and
research findings".
The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function
relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and
including trouble shooting.

Tentative Technical Program Schedule (as of December 3rd, 2001)

Sunday, May 5th
Short Course - Understanding structure-function relationships in food systems through specific
localisation methods and microscopy. Contact: Marcel Paques (Paques-at-nizo.nl)

Monday, May 6th-Morning
Opening of symposium - Opening remarks

Plenary Speaker and presentation of Division Achievement Award

Dairy Applications Session. Chairs: Mark Auty, Dairy Products Research Centre, TEAGASC,
Ireland (mauty-at-moorepark.teagasc.ie ) and Harjinder Singh, Massey University, NZ (H.Singh-at-massey.ac.nz)

Some Observations of a Microscopist, Author, and Reviewer on Structural Studies of Milk Products. M. Kalab, Agriculture and Agri-Food Canada, Canada

Effect of Emulsifiers and Processing Conditions on Microstructure of Milk Fat/Sunflower Oil Blends. S. Martini1, M. Cerdeira1, C. Puppo1, R.W. Hartel2, and M.L. Herrera1,3, 1CIDCA, UNLP, CONICET, Argentina; 1University of Wisconsin-Madison, USA; 3University of Buenos
Aires, Argentina

Texturization of Dairy-Based Spreads. Y. Shi, B. Liang, and R.W. Hartel, University of Wisconsin-Madison, USA

Localization of Whey and Casein in Cheeses Using Microscopy and Immunochemistry Techniques. Y. Wang and D. Pechak, Kraft Foods, U.S.A

Manufacturing Yoghurt Structures with a Predicted Consumer Preference. M Langton and A. Astrom, SIK, Sweden

Dynamic Confocal Imaging of Tension and Fracture in Composite Food Materials. D.P Ferdinando1, K.P Plucknett2, and V. Normand3, 1Unilever Research, UK; 2DERA, UK; 3Firmenich SA, Switzerland

TBA - Topic: Dairy powders/caramels. C. Attapattu, University of Wisconsin, USA

Monday, May 6th - Afternoon
Colloidal and Interfacial Sciences Session.
Chairs: Marcel Paques (Paques-at-nizo.nl ) and David Pechak (Dpechak-at-kraft.com)

Protein Polysaccharide Interactions. C.G. De Kruif, NIZO Food Research, Netherlands (keynote speaker)

To Be Announced. B. Campbell, Kraft Foods, USA

Structure in Heat Treated Low_Fat Emulsions. R. Ofstad and V. Hoest, MATFORSK, Norway

Fatty Acid Salts-Induced Gels of Food Proteins: Their Rheological Properties and Structural Changes of the Proteins During Gelation. N. Yuno-Ohta, Nihon University, Japan

Wheat Gluten Proteins. A.S. Tatham, IACR Long Ashton research Station, United Kingdom

Interfacial Composition and Stability of Oil in Water Emulsion formed with Mixtures of Milk Proteins and Polysaccharides. H. Singh and Y. Hemar, Institute of Food, Nutrition and Human Health, Massey University, NZ

Dedicated Poster Session

Division Board Meeting

Tuesday, May 7th - Morning
Agricultural Applications of Microscopy and Imaging Session/ joint with Feed
Microscopy Division. Topic: Food Contamination contacts: Mark Auty, Dairy Products Research Centre, TEAGASC (mauty-at-moorepark.teagasc.ie ) and Marge McCutcheon, West Virginia Department of Agriculture, USA (Feed Microscopy Division)

Contaminants in Food Processing. D. Kittleson, General Mills Technology East , USA

How to approach contaminant identification. M. Auty, Dairy Products Research Centre, Ireland

Identification of plant material. D.F. Wood, USDA, USA

To Be Announced. J. Makowski, Windsor and Associates, USA

Species identification of Animal Hair by Using Atomic Force Microscopy. C.W. Cruywagen, University of Stellenbosch, South Africa.

Quanitation of Total Fat and Fat Quality in Cheese and Dairy Products Using Membrane Separation Technology. V.C. Gordon, Safety Associates, Inc., USA

Detection and Differentiation of APRALAN, PAYLEAN, PULMOTIL and TYLAN in Animal Feeds using microscopy. P. Klink. Elanco Animal Health, a Division of Eli Lilly and Company, USA.

Additonal speakers to be announced.

Division Luncheon and round table (expert) discussion. Topic to be announced

Tuesday, May 7th - Afternoon
Microbiology and Food Session Chairpersons: Judy Arnold and Ida Yates, USDA, ARS, Russell Research Center, USA

Prevention of Bacterial Fouling on Food Equipment Surfaces. J.W. Arnold, USDA, ARS, Russell Research Center, USA

Probiotics and Their Use in Food Animal Production. R. Droleskey, USDA, ARS, SPARC, USA

The Effect of High Pressure Steriliztion on Listeria Inoculated Seafood. K.R.S. Schneider and M.V.W. Wood, University of Florida, USA

Food Microstructure Investigations by Atomic Force Microscopy. J. Thornton, Digital Instruments/Veeco Metrology Group, USA

Controlling Growth of the Toxigenic Fungus, Fusarium Verticillioides. I. Yates and J. Arnold, Russell Research Center, ARS, USDA, USA

Division Members Meeting (immediately following the afternoon session)

Wednesday, May 8th- Morning
Ingredients and Food Processing Session- Chairpersons: Diana Kittleson, General Mills Technology East, USA; and Bernhard Tauscher, Federal Research Center for Nutrition, Germany

Non-Invasive Quality Determination of Fruit and Vegetables: Application of a Multi-Wavelength NIR-Diode Laser Array. B. Tauscher, Federal Research Center for Nutrition, Germany

Characterisation of the Application of Novel Oil Structurants. E. Floeter, F. Gandolfo, and W. Hogervorst, Unilever Research Vlaardingen, The Netherlands

High Pressure Processing. E. Ting and E. Raghubeer, Flow International, USA

Microstructure of Rice Starch Isolates. D.F. Wood1, A.M.Ibanez_Carranza2, and C.F. Shoemaker2, 1USDA, ARS, WRRC, USA; 2University of California, USA

To be announced, D.W. Stanley, Department of Food Science, University of Guelph, Canada

To Be Announced. F. Escher, B. Conde-Petit, ETH, Switzerland

To Be Announced. M. Michel, Nestec Ltd., Nestle Research Center, Switzerland

To Be Announced. M. Salmenkallio-Marttila , VTT Biotechnology, Finland

To Be Announced. J. Boye, Agriculture and Agri-Food Canada, Canada.

Wednesday, May 8th - Afternoon
New Methods and Techniques for Food Structure and Functionality Analysis SessionChairpersons: Kathy Groves, Leatherhead Food Research Association, England; and Maud Langton, SIK, Sweden

Quantifying Microstructures through Image Analysis. G.M.P. van Kempen1, M. van Ginkel2, C.L. Luengo Hendriks2, L.J. van Vliet2, and S. Singleton3, 1Unilever Research Vlaardingen, Netherlands; 2Delft University of Technology, The Netherlands; 3Unilever Research Colworth House, Great Britain

Cryo-TEM of Biopolymers in Comparision with Other TEM-techniques. M. Langton, A. Altskar, and A.-M Hermansson, SIK, Sweden

Freeze-substitution and low temperature embedding of dairy products for electron microscopy. A.K. Smith and H.D. Goff, Department of Food Science, University of Guelph, Canada

MicroRheology: preliminary results of structural behaviour of foods under deformation.
M. Paques, Y. Nicolas, Wageningen Centre for Food Sciences/Unilever Vlaardingen, The Netherlands.

Recent Advances in our Understanding of the Relationship Between Crystallization Behavior, Microstructure and Rheological Properties of Fat Crystal Networks. A. Marangoni, University of Guelph, Canada

Spectroscopic Prediction of Rheological Properties in Grains. F. Meadows and F. Barton USDA, ARS, QARU, USA

Staining Techniques for Detection of Components in Fish Muscle. K. Hanneson Eggen and G. Enersen, Matforsk, Norway

Changes in plant tissue after pulsed electric field treatment. M. Fincan, P. Dejmek, Dept. of Food Engineering, Lund University, Sweden

Closure of Symposium

Posters

Relationships between Microstructure and Rheological Properties of Model Lipid Systems. B. Liang, Y. Shi, and R.W., Hartel Univ. of Wisconsin-Madison, USA

Minor Biomolecules from the Olive Drupe to Olive Oil: The Technology and the Well-being Effects. N. Uccella, CIRASAIA-Mediterranean Agrifood Research Centre, Calabria University, Italy

Formation and Physical Properties of ?-Fat Gel. I: Macroscopic and Microscopic Observations. K. Higaki1, Y. Sasakura1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. Ii: In situ Observation of Gel-Formation Processes. K. Higaki1, Y. Sasakura1, I. Hachiya1, S. Ueno2, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. III: Rheological Properties. K. Higaki1, T. Koyano1, I. Hachiya1, and K. Sato2, 1Meiji Seika Kaisha Ltd., Japan; 2Faculty of Applied Biological Science, Hiroshima University, Japan

Formation and Physical Properties of ?-Fat Gel. IV: Why and How? K. Sato1, K. Higaki2, T. Koyano2, and I. Hachiya2, 1Faculty of Applied Biological Science, Hiroshima University, Japan; 2Meiji Seika Kaisha Ltd., Japan

Exchange in Semi-Solid Triglyceride systems measured by NMR Spectroscopy: Effect of Partial Glycerides on Exchange Rates. P. Smith1, N. Haghshenas1, I. Furo2, and B. Bergenstahl3, 1YKI Institute for Surface Chemistry, Sweden; 2Royal Institute, Sweden; 3Lund University, Sweden.

Effect of Shear Rate on Fat Crystallization Kinetics. P.H. Rousset and V. Mooser, Nestle Research Center, Switzerland.

Utilizing Polarized Light Microscopy to Characterize the Effects of Tween60 on the Physical Properties of a Model Plastic Fat System. J.W. Litwinenko and A.G. Marangoni, University of Guelph, Canada.


From daemon Mon Dec 3 14:39:45 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 03 Dec 2001 15:32:40 -0500
Subject: Re: Negative Fixing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


AnnFook Yang may have gone to the experts, but do they know their own products?

The material known today as Sodium Thiosulphate (or sodium thiosulfate),
with the composition Na2S2O3.5H2O was formerly known as sodium
hyposulphite. As it was the principal ingredient in fixers, the
conventional abbreviation for the fixing bath was "Hypo". The chemistry
hasn't changed - just the name has been forgotten, but not by Kodak, who
still manufacture (or at least, did last time we ordered it!) "Hypo-Clear",
which is a rapid washing agent.

Kodak's current Rapid Fixer (which isn't listed on their Web site, but is
referred to in the literature accompanying their current black-and-white
photography products, and which we and others still buy in significant
quantities) uses Ammonium Thiosulphate rather than the sodium salt. The
same literature also specifies the use of Kodak Fixer, which presumably
therefore is still available, and which I imagine is still a sodium
thiosulphate (or "Hypo"!) based fixer, but I don't have any on hand, so
can't be sure of that.

Interestingly, Ilford's web pages also do not mention their black-and-white
fixers - only by going to the product specification pages can one find
mention of the Ilford line of B&W chemicals - as asides in the processing
recommendations. Is this some sort of conspiracy to render the market
obsolete by denying users any information about the products, thus ensuring
that the demand will wither away?

For our film - SO163 in our case - we use the exact sequence that the Kodak
support person recommended, as we have for the past 25 years. For paper,
on the rare occasions when we make photographic enlargements, we add the
step of soaking in Hypo-Clear.

Tony Garratt-Reed



} I have never used Orbit bath nor Hypo. In fact the former is totally new
} to me. I have heard of Hypo in the '60s. I was curious and went to the expert.
} I phoned Kodak Scientific Support (800-225-5352) to find out whether
} developing the 4489 film in D19 and fixing in Kodak Rapid Fixer is
} adequate. The answer is "Yes"
} What about Hypo? He asked, "Who makes it?"
} He assured me that nothing else is needed after Rapid Fix. Just wash the
} negs. in water will do.
}
}
}
}
}
} AnnFook Yang
} EM Unit,
} Eastern Cereal and Oilseed Research Centre,
} Room 2091, Bldg. 20,
} Central Experimental Farm,
} Ottawa, Ontario
} Canada K1A 0C6
}
} Tel: 1-613-759-1638
} Fax: 1-613-759-1701
}
} e-mail: yanga-at-em.agr.ca
}
} } } } "Paula Sicurello" {patpxs-at-gwumc.edu} 11/30/01 03:25PM } } }
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
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** Fax: (+) 1-617-258-6478
**




From daemon Mon Dec 3 16:56:36 2001



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Mon, 03 Dec 2001 14:48:38 -0800
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Catherine,
a microtome devoted to polymers should have a cryo chamber since a lot of
polymers are easier to section at sub-zero (C) temperatures. Definitely
recommend a 35 deg knife.
Other than that, just use common strategy for microtomy of biological
samples (with some patience for trial/error temp setting). You can contact
me off-line for our instrument info. Good luck,

Alice.

Alice Dohnalkova
Battelle, PNNL
Richland, WA
(509)372-0692

-----Original Message-----
} From: "Catherine.Derr-at-gastechnology.org"-at-sparc5.microscopy.com
[mailto:"Catherine.Derr-at-gastechnology.org"-at-sparc5.microscopy.com]
Sent: Monday, December 03, 2001 6:20 AM
To: Microscopy-at-sparc5.microscopy.com


I looking at purchasing a microtome for sectioning polymers, primarily
PE. Although I am familiar with microtomes used in biological studies, I
have
no experience in sectioning polymers. Has anyone sectioned polymers with
repeated success? What features are desired for sectioning polymers? Is
there a specific knife angle that works well?

KD Derr
SEM/Polymer Technician
Gas Technology Institute
1700 S Mount Prospect Rd
Des Plaines, IL 60018
(847) 768-0505 (phone)
(847) 768-0569 (fax)
kd.derr-at-gastechnology.org





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From daemon Mon Dec 3 21:38:37 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 03 Dec 2001 22:26:52 -0500
Subject: microtomy of polyethylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Catherine Derr wrote:
======================================================
I looking at purchasing a microtome for sectioning polymers, primarily PE.
Although I am familiar with microtomes used in biological studies, I have no
experience in sectioning polymers. Has anyone sectioned polymers with
repeated success? What features are desired for sectioning polymers? Is
there a specific knife angle that works well?
======================================================
We have been sectioning polyethylene for over thirty years in our laboratory
. If you are talking about thin sectioning polyethylene, unpigmented, then
in many ways, the sectioning is not going to be greatly different from your
biological samples.

There are several things that are going to be very important for you:

1] PE sectioning can not be done at room temperature, only cryo.

2] polyethylene is very soft, and the key is to do the sectioning with
minimum distortion. That means diamond knives, and the smaller the angle
the better. We usually are using a 35° knife angle.

3] polyethylene is either injection molded (into parts) or melt extruded
(into film). The results you get from sectioning along the injection or
extrusion direction might not necessarily be the same as when you section
transverse to that section. So you want to give some thought as to which
view would give you the most useful information considering your objectives
and then it would be most important to always section all samples at the
same angle relative to the machine direction. Otherwise, differences in
section angle could be "interpreted" as differences between samples but such
would be false interpretations.

4] pure unfilled polyethylene are normally pretty unremarkable by thin
section TEM because there is nothing really to "see" or to give any contrast
. Unless you were trying something exotic with the sections, such as
negative staining of the lamellar or spherulytic structures, your main
concern would be in the sectioning of the inorganic additive particles which
in general would be much harder than the polyethylene. The presence of
these additives would impart small damge points on the knife edge which
would then show up as annoying striations in the final section (and image).
Therefore you don't want to use your good (and expensive) life science
knives for such sectioning, but a good materials science knife (which can be
obtained through SPI or the other firms offering diamond knives.

5] We have found that when comparing microtomes from the two major
ultramicrotome manufacturers, one can get comparable results using either
system. Just remember that for this kind of work, a good cryo system is
needed.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


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From daemon Tue Dec 4 02:04:11 2001



From: Grup Epigenetica :      planaria2000-at-yahoo.com
Date: Mon, 3 Dec 2001 23:57:15 -0800 (PST)
Subject: help with confocal assistant

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers

I would like to do 3D reconstructions out of my .avi or .tif series, is
it possible to do so with the Confocal Assistant ? If not, what
easy-to-handle software is available?

Thank you


Albert Cardona
Genetics Department
University of Barcelona
Av. Diagonal, 645 08028 Barcelona, Spain

__________________________________________________
Do You Yahoo!?
Buy the perfect holiday gifts at Yahoo! Shopping.
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From daemon Tue Dec 4 02:36:02 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 4 Dec 2001 08:40:23 +0000 (GMT Standard Time)
Subject: Re: anodising vacuum components

Contents Retrieved from Microscopy Listserver Archives
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Hi Fredrick

As a follow up to your posting on anodised componenets in
vacuum - decorative anodising is often wiped with a light
oil or polish, after dyeing, to give that nice shiny look.

Now that really isn't good for the vacuum!!!!

Regards,
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Tue Dec 4 06:58:50 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Tue, 4 Dec 2001 22:53:49 +1000
Subject: RE: Cleaning SEM stubs - reply to all correspondence

Contents Retrieved from Microscopy Listserver Archives
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For crying into a big bucket. Under the onslaught I have no doubt that normal
heavy duty anodized surface (like a handrail) are unsuitable for SEM mounts.
} Most aluminium anodization that I have seen creates a dark
} THICK oxide film (tens to hundreds of nm thick) and is insulating. (Nestor).

But I never suggested that the mounts were heavily anodized; on the contrary
I stated that the coating on our mounts is very thin. Furthermore, I stated
that the appearance of our mounts is identical to those from other suppliers.
Recently, because of a lost shipment we had to buy some American-made mounts
and I'm sure that no customer would have noted.

I am not an expert on anodizing either, but when many years ago I sent samples
to our manufacturer, I was immediately advised - "they are anodized". So we had
ours "anodized", afterall the samples had come from Chuck!

I find it amazing that, seemingly same listers believed that I would supply
dull, thick anodized mounts which don't conduct and outgas catastrophically. My
previous two communications, made it clear that that just wasn't so.

The issues may be: Is our supplier electropolishing, but calls that anodizing?
Or how would in practice a very thin layer of anodizing differ from the
naturally occurring oxide film? Or what is the end result of electro-polishing?
Could this in fact add a thin anodized layer? Is a very thin anodized film
shiny or dull?

I would have liked answers to those questions, which could have resolved the
differences. At this point I know that the mounts are not just machined Al;
they had a treatment and according to the manufacturer they are "anodized".
There is of course the possibility that our manufacturer never applied any
process. But why would he? The charge for "anodizing" is paltry.
Thank you for your vigorous discussion.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, December 04, 2001 4:01 AM, Frederick Schamber
[SMTP:schamber-at-aspexllc.com] wrote:
} Jim,
} I will be the first to admit that I am not an expert on either SEM stubs or
} anodizing, but I have had very negative experience with the latter in vacuum
} systems. I once designed some relatively small black-anodized parts into a
} SEM and spent considerable time trying to find out why we had such bad
} "outgassing" -- finally, someone wiser than me sanded off the anodizing and
} the vacuum was fine. So I have, in effect, done the experiment you describe
} -- ordinary machined aluminum parts are vastly superior to anodized aluminum
} parts in terms of their vacuum properties in my experience (and I did some
} followup experiments in a well-controlled apparatus designed specifically to
} measure vacuum properties of materials and confirmed that a little bit of
} anodizing is a very bad thing). It is not a question of the mere "roughness"
} of the surface. Apparently the anodizing process creates very tiny
} interstices which hold the dye, and this is what causes the problem -- or so
} I have assumed. I suppose it could be the dye itself -- I never tried the
} experiment on "clear anodized" parts.
} When I look at catalog advertisements for aluminum SEM stubs, I always see
} reference to various degress of polishing, and don't recall ever seeing
} reference to anodizing.
} From an electrical standpoint, I also really wonder how anodizing could work.
} I know that anodizing must be avoided on electrical cabinetry because one
} cannot establish reliable contacts (a different surface treatment process is
} used on aluminum instead). I'm sceptical about the "thin coating" concept
} since it is my understanding that it is precisely the insulating layer which
} protects the surface from oxidation. Any amount of insulation on a SEM stub
} would be a big problem. (Of course, the same thing can be said about the thin
} oxide layer that naturally forms when aluminum is exposed to air.) But it is
} my understanding that anodizing is a process which modifies the surface, not
} a simple "coating" which can be "thin".
} My negative opinions about anodizing for vacuum systems aren't just mine --
in
} fact, I thought they were "common knowledge". For example, I refer you to the
} very informative vacuum information site maintained by Roy Schmaus at the
} University of Alberta
} ~http://nyquist.ee.ualberta.ca/~schmaus/vacf/index.html
} {http://nyquist.ee.ualberta.ca/schmaus/vacf/index.html}
} Under Basics/an Introduction to Materials for use in Vacuum we find the
} following statement: "Aluminum that will be exposed to vacuum should never be
} anodized due to serious outgassing problems." Under "References" on the same
} page he notes: "I recently had a call from someone who has used Anodized
} Aluminum as an insulator down into the ultra high vacuum region. Admittedly
} the pieces used were very small and initial outgassing was a problem but low
} costs were a major advantage." [emphasis added]
} So given my negative experience and the congruent remarks of others who work
} in this field, I have long assumed that anodizing was something to avoid at
} all costs in vacuum systems -- and I've assumed that applied to SEM stubs. Am
} I wrong?
} How do polished stubs stay so shiny? I've wondered the same. However,
anodized
} parts I have seen always have a kind of "matte" appearance, rather than a
} high gloss in any case.
} So, though it is entirely possible that I will learn something from this
} discussion that I did not know previously (it would hardly be the first
} time), my limited grasp of the subject still convinces me that SEM stubs
} should NOT be anodized. But how do they retain their surface finish and
} conductivity? Your response suggests that you manufacture SEM stubs and call
} out an anodized surface -- did I read this correctly? Or are you purchasing
} stubs which you assume are anodized? I really would be interested if someone
} who is in the business of manufacturing these things could comment directly
} to this -- without disclosing proprietary information of course.
} "Charity" in view of the season notwithstanding, I consider this a matter of
} considerable practical importance. If I am in error about this, I want to
} know it, since a non-insulating, non-outgassing, but still protective
} anodizing surface would be a very good thing. If I am correct, then others
} reading this exchange should know that anodizing is something that should be
} scrupulously avoided in vacuum systems (SEM and otherwise). In either case,
} getting to the bottom of this will presumably be a good thing for everyone.
} I'm prepared to learn from new facts, if someone can put them on the table.
} Fred Schamber
} ASPEX, LLC
}
} Jim at Proscitech wrote:
} You are hoping in vain, commercial mounts are anodized, because:
} 1 The layer is too thin to insulate even at low voltages. SEM have high volts
}
} (even specimen currents are many and not fractions of volts) and low
currents.
}
} No problem.
} 2 Otherwise the mounts would oxidise and I believe that too is not the best
} conductor.
} 3 Al too is porous, if you really care use polished stainless.
} 4 Does it matter? It would in an ion pumped TEM, but in an SEM?
} 5 All other suppliers do anodize. That is a better reason than you may think!
}
} 6 If we did not, people would buy other supplier's mounts because ours would
} look "cheap"
} Now, I have a question: How long have you and some other people been using
} anodized mounts and not realised that the nice shiny finish is not a polish.
} Have you never seen machine finished Al???
} If you really want to make a point, place a hundred commercial mounts into
the
}
} bottom of the SEM chamber and then repeat that experiment with machine
} finished
} Al mounts. Record vacuua at various points and run the experiment three
times.
}
} Report the results. I would be interested to learn if there is any
difference.
}
} Those hundred plain Al mounts made in a machine shop would be a trifle
} expensive (you could acid strip the anodizing), but cheaper than most
} experiments.
} Christmas is coming, so lets be charitable: anodized mounts are a good deal
} and
} they are very suitable for SEM work.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
} On Monday, December 03, 2001 12:14 PM, Frederick Schamber
} [SMTP:schamber-at-aspexllc.com] wrote:
} } I would certainly hope that SEM stubs are NOT anodized. Not only does
} } anodizing
} } create an insulating surface, but it also creates a surface with very poor
} } vacuum
} } properties -- anodized parts will pump down slowly due to the gas trapped
in
} }
} } the
} } porosity.
} }
} } Fred Schamber
} } ASPEX, LLC
} }
} } Jim at Proscitech wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
} } }
} } } -----------------------------------------------------------------------.
} } }
} } } I'm certain that every mount sold by ProSciTech is anodized, and by their
} } }
} } } very
} } } appearance, all other such commercially sold mounts I have seen are
} } } anodized
} } } too. True the anodized film is very thin.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } ABN: 99 724 136 560 www.proscitech.com
} } }
} } } On Saturday, December 01, 2001 9:07 AM, Darrell Miles
} } } [SMTP:milesd-at-US.ibm.com]
} } } wrote:
} } } }
------------------------------------------------------------------------
} } } }
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } }
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } }
} } } } On-Line Help
} } } } {http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
} } } }
-----------------------------------------------------------------------.
} } } }
} } } }
} } } }
} } } } Anodizing of aluminum can be done in many colors, as
} } } } well as a clear coating. I would not expect SEM stubs to be
} } } } anodized, as the aluminum oxide coating which forms is an
} } } } insulator. This would inhibit the removal of the electron
} } } } charge from the sample. The native oxide film is no where
} } } } near the thickness of the usual anodized film, and can easily
} } } } be broken. The thickness of the anodized film can be
} } } } controlled, but it is still an effective insulator. I am fairly
} } } } certain that none of the stubs I have ever used were anodized.
} } } }
} } } } Regards,
} } } } Darrell



From daemon Tue Dec 4 07:30:17 2001



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 04 Dec 2001 08:24:27 -0500
Subject: Negatives in Orbit

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Hi Listers,

The consensus on my question was HUH? Most of the EM world has used a hypo clearing agent after the fixer step to reduce the washing time. Though I did get a reply stating that is was used originally to reduce the time in fixer and it was supposed to make for better archival properties.

I will be returning to the tried and true method as soon as it gets here.

Thanks to all who replied.

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax



From daemon Tue Dec 4 08:05:44 2001



From: Paul.Nolan-at-alcan.com
Date: Tue, 4 Dec 2001 08:58:31 -0500
Subject: SEM stubs

Contents Retrieved from Microscopy Listserver Archives
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Just to clear up a couple of statements someone made.
There is no such thing as a "clear" anodic film.
And dies are not used to give anodic films colour ..The colour comes from
metals in the anodic layer. Thickness of the layer is also a factor.

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Tue Dec 4 09:01:24 2001



From: Rotole, John A. :      John.Rotole-at-ispat.com
Date: Tue, 04 Dec 2001 08:28:41 -0600
Subject: Aluminum metal - native oxide thickness

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Good Morning everyone,

The native oxide thickness of aluminum metal, depending upon processing, is typically on the order of 30 to 50 angstroms. You can measure this thickness very easily by making a simple XPS measurement of the Al2p core level.

For more information, I would recommend:

B.R. Strohmeier, Surface and Interface Analysis, Volume 15, pgs 51 - 56 (1990).

Cheers,

John


John A. Rotole, Ph.D.
Project Engineer
Coatings & Surface Technology
Ispat Inland Product Research
3001 E. Columbus Drive
East Chicago, Indiana 46312
Tel: 219-399-6308
Fax: 219-399-6562



From daemon Tue Dec 4 09:12:08 2001



From: Paul.Nolan-at-alcan.com
Date: Tue, 4 Dec 2001 10:06:12 -0500
Subject: adhesive

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Hello

I'm looking for a double sided adhesive to stick samples (aluminum) onto
glass slides.
I want to be able to remove them with relative ease and replace them for
storage.
Conductivity is not an issue
At present i am using those little double sided sticky press down adhesives
used for SEM stubs ..they are a little to sticky for my liking.

Any suggestions?

Paul D. Nolan
Electron Optics

Alcan International Limited
Kingston Research and Development Centre
P.O.Box 8400, 945 Princess Street
Kingston, Ontario K7L 5L9

Tel: (613) 541-2066
Fax: (613) 541-2134
paul.nolan-at-alcan.com



From daemon Tue Dec 4 09:30:35 2001



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Tue, 04 Dec 2001 10:22:22 -0500
Subject: Re: microtoming PE

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} With any instrument purchase the best thing to do is go to the
} manufacturers, or better yet have them come to you, and see how the
} instrument works on your samples. I have extensive experience sectioning
} PE and I have always used Reichert-Jung, now Lieca, ultramicrotomes. That
} does not mean others would not do the job. The main thing is to get a
} system that works well when cryo-ultramicrotoming. As stated in another
} response you may well have to cryotome your samples and if you are buying
} for polymer work you definitely want to get a system that will work at
} both room temp and cryo conditions. How to section PE can differ
} depending on the type of PE, high density or low density. If you have
} HDPE then you can often pre-stain with Chlorosulfonic Acid and room temp
} microtome. Otherwise you may have to stain with RuO4 before or after
} cryomicrotoming. There are many good references on these
} techniques. Good luck on your purchase.


} I looking at purchasing a microtome for sectioning polymers, primarily
} PE. Although I am familiar with microtomes used in biological studies, I
} have
} no experience in sectioning polymers. Has anyone sectioned polymers with
} repeated success? What features are desired for sectioning polymers? Is
} there a specific knife angle that works well?
}
} KD Derr
} SEM/Polymer Technician
} Gas Technology Institute
} 1700 S Mount Prospect Rd
} Des Plaines, IL 60018
} (847) 768-0505 (phone)
} (847) 768-0569 (fax)
} kd.derr-at-gastechnology.org

Stephen McCartney
Research Associate
2108 Hahn Hall
Materials Institute
Virginia Tech
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Tue Dec 4 11:21:19 2001



From: Petersen, Maureen A. :      Mape-at-mail.ifas.ufl.edu
Date: Tue, 4 Dec 2001 12:13:27 -0500
Subject: RE: artifacts and blunders

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Being a Plant Pathologist, the best I can think of is the story of Pierce's
disease of grapes, which was of unknown etiology for a long time. Forgive me
for not seeking details from the literature.

At one time it was proposed to be caused by a virus. Extensive light
microscope work missed the true cause- a bacterium. After elucidation of the
pathogen, I am told that upon review of work previously done, the bacterium
WAS there to be seen, but was missed.

Upon a similar vein, MLO's in plants were not recognized in TEM work until a
human or animal pathologist saw micrographs of MLO's in plant tissue, and
was readily able to say what they were.

Anecdotal, but food for thought.

Lesson? I better keep my eyes and my mind open.

Maureen Petersen
Dept. Plant Pathology
University of Florida


From daemon Tue Dec 4 11:49:43 2001



From: robert.fowler-at-tdktca.com
Date: Tue, 4 Dec 2001 12:45:32 -0500
Subject: Aluminum Stubs

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Dear Listers,
Given that my curiosity is up per the plating/no plating of the stubs, my
posting is geared more towards the actual reusing of stubs. For the past
eight years I have been subjecting a set of 50 or so stubs to a sand
blasting treatment. You may question the feasibility of a sandblaster BUT
what is important is the media used to blast with. glass beads in the range
of 170-325 mesh are used to effectively remove residue, glue, tape, and any
material that resides on the stub. This particular media results in no
residue and no tolerance change, although a pure water treatment and
ultrasonic wash are utilized. I have had no trouble at all even after this
amount of time.

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



From daemon Tue Dec 4 11:53:31 2001



From: Joyce Craig :      J-Craig-at-csu.edu
Date: Tue, 04 Dec 2001 11:40:53 -0800
Subject: cross sectioning hair

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I embed the hair in epoxy after the usual fixation and dehydration
steps. Then section with a glass knife, mount on a slide, and stain
with toluidene blue.
Joyce Craig
Chicago State University



From daemon Tue Dec 4 13:04:56 2001



From: Mike Nesta :      MNesta-at-ebsciences.com
Date: Tue, 4 Dec 2001 13:57:22 -0500
Subject: Al SEM Stubs: is it Anodized or Not....

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Dear Nestor,

You are right about an anodized aluminum finish. It is an excellent
insulator, not at all the type of finish you want on an SEM specimen mount,
as conductivity is critical. I think it may also create vacuum problems.

The mounts (stubs) we manufacture are machined on very high speed turning
equipment from high grade aluminum bar stock. After machining, we put them
through a rigorous part cleaning process using special cleaning solutions.
Typical chemical based solutions tend to leave a film on the mounts that
both insulates and degrades vacuum. Event our turning equipment uses a
special, non-petro based cutting lubricant to avoid the potential for any
contamination. Each mount is then carefully inspected and wiped down to
ensure that they are completely clean and dry, then stored in air tight
packing material.

Because our machines operate at such high speeds, our process eliminates the
need for polishing. However, mounts that are made on more conventional
lathes or machines may require a final polishing to achieve the same
results.

I hope this helps.

Best regards,
Michael R. Nesta
General Manager
Energy Beam Sciences, Inc.
Agawam, MA USA
Tel: (413) 786-9322
"Adding Brilliance to Your Vision"



-----Original Message-----
} From: zaluzec-at-sparc5.microscopy.com
[mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Monday, December 03, 2001 9:32 AM
To: Microscopy-at-sparc5.microscopy.com



As I recall from my metallurgy

To "Anodize" is to subject a metal to an electrolytic action as the
anode of a cell in order to coat/deposit a protective or decorative film.

Most aluminium anodization that I have seen creates a dark
THICK oxide film (tens to hundreds of nm thick) and is insulating.

I've never seen this on an aluminium SEM stub. Certainly many I've used are
electropolished and some (the ones we make here in our machine shop )
are simply milled and mechanically polished. All will at a minimum
have a native oxide.
All are conductive, and yes (Jim) I've seen polished
(electrochemical, mechanical) as
well as all ranges of "machine" finished products. These are NOT anodized,
they
are all simply oxidized by leaving them sit in air. The native oxide
which forms
is a few tens of angstroms thick and is stable, by definition this is not
anodization. I have never heard of anyone calling this process
"anodization"
should that be what you are referring to.

So... now I'm curious and I would request that the various suppliers
who like reply
to this list to fill us all in on what you do.

1.) How many of you anodize your stubs (ie. coat), vs electropolish,
vs mechanically polish.
2.) If you intentionally anodize, (ie. deposit/coat) your stub with
an electrodeposited layer
how thick is your film, and what do you anodize it with.
3.) It might also help to define the "anodization" process (if you do
this) so that we are all
understand what each of you mean in the context of how you make the
stubs we purchase.

Nestor
Your Friendly Neighborhood SysOp.





From daemon Tue Dec 4 14:36:30 2001



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Tue, 04 Dec 2001 14:29:10 -0600
Subject: envelopes

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we inadvertantly ordered several thousand 4x5 inch glassine envelopes
(for EM negatives). As we are not using this size and I would hate to
waste them, anybody could have them for free. I am at the University of
Chicago. Please contact me offline.

Christoph


From daemon Tue Dec 4 15:14:02 2001



From: Chris Jeffree :      c.jeffree-at-ed.ac.uk
Date: Tue, 4 Dec 2001 21:10:52 -0000
Subject: Re: SEM stubs

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Paul
Is there a distinction between an anodic film and an anodised film??

Organic dyes are in fact widely used to colour anodized aluminium,
though I am prepared to concede that I am not up to date with
techniques for anodizing, and may have missed recent innovations
involving metal-doping of the oxide film.
The aluminium oxide film formed on anodized aluminium at first has a
frosted appearance, which is
presumably caused by light scattering from multiple voids and
micro-rough surfaces.
The film is rendered more translucent by various post-anodizing
treatments, following dyeing if desired. But once this treatment is
carried out, the film is clear - i.e. you can see right through to the
base metal, aluminium oxide being one of nature's clear,
light-transmitting materials.
Most people have experienced lightfastness problems in dyed anodized
aluminium products, a syndrome which I am not aware occurs in
metal-doped alumina such as ruby.
I have seen the anodizing process being carried out, including dyeing
with organic dyes, without any other metal than aluminium in sight.
Was I dreaming this?

Chris

} Just to clear up a couple of statements someone made.
} There is no such thing as a "clear" anodic film.
} And dies are not used to give anodic films colour ..The colour comes
from
} metals in the anodic layer. Thickness of the layer is also a factor.
}
} Paul D. Nolan
} Electron Optics
}
} Alcan International Limited
} Kingston Research and Development Centre
} P.O.Box 8400, 945 Princess Street
} Kingston, Ontario K7L 5L9
}
} Tel: (613) 541-2066
} Fax: (613) 541-2134
} paul.nolan-at-alcan.com
}
}



From daemon Tue Dec 4 16:36:57 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Dec 2001 17:34:59 -0500
Subject: Re: Aluminum Stubs

Contents Retrieved from Microscopy Listserver Archives
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Robert,
I also bead-blast stubs. Works very well as long as you're not looking
for a smooth surface, then you have to grind and polish afterwards, but
the bead blasting DOES clean that top surface in a hurry. On Al I
usually only blast at 35psi to keep the erosion down.

I have several pieces of Al that have a lot 1/8" holes drilled in them.
Load'em up with stubs and blast away! It's fast, it's fun and
reasonably ecologically sound. Follow with a BRIEF ultrasonic bath in
JOY, rinse with tap, DI or distilled water, depending on how particular
you are, and dry. Alconox and some of the other heavy-duty lab cleaners
have a pH that is far too high for Al, although they work great on glass.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} Given that my curiosity is up per the plating/no plating of the stubs, my
} posting is geared more towards the actual reusing of stubs. For the past
} eight years I have been subjecting a set of 50 or so stubs to a sand
} blasting treatment. You may question the feasibility of a sandblaster BUT
} what is important is the media used to blast with. glass beads in the range
} of 170-325 mesh are used to effectively remove residue, glue, tape, and any
} material that resides on the stub. This particular media results in no
} residue and no tolerance change, although a pure water treatment and
} ultrasonic wash are utilized. I have had no trouble at all even after this
} amount of time.
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}
}
}



From daemon Tue Dec 4 20:39:58 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 04 Dec 2001 21:32:59 -0500
Subject: SEM Mount Production

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael R. Nesta wrote:
===============================================================
You are right about an anodized aluminum finish. It is an excellent
insulator, not at all the type of finish you want on an SEM specimen mount,
as conductivity is critical. I think it may also create vacuum problems.

The mounts (stubs) we manufacture are machined on very high speed turning
equipment from high grade aluminum bar stock. After machining, we put them
through a rigorous part cleaning process using special cleaning solutions.
Typical chemical based solutions tend to leave a film on the mounts that
both insulates and degrades vacuum. Event our turning equipment uses a
special, non-petro based cutting lubricant to avoid the potential for any
contamination. Each mount is then carefully inspected and wiped down to
ensure that they are completely clean and dry, then stored in air tight
packing material.

Because our machines operate at such high speeds, our process eliminates the
need for polishing. However, mounts that are made on more conventional
lathes or machines may require a final polishing to achieve the same results

From daemon Wed Dec 5 01:02:03 2001



From: Kristian Ukkonen :      kristian.ukkonen-at-iki.fi
Date: Wed, 05 Dec 2001 08:55:46 +0200
Subject: Re: SEM stubs

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"Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com wrote:
} Just to clear up a couple of statements someone made.
} There is no such thing as a "clear" anodic film.
} And dies are not used to give anodic films colour ..The colour comes from
} metals in the anodic layer. Thickness of the layer is also a factor.
} Paul D. Nolan

The anodizing process for aluminium indeed does require a dye.
There are plenty of companies selling the dyes etc..
http://www.caswellplating.com/frames.asp?bottom=/anodizedye.htm
http://www.plating-supplies.com/Anodizing.html
http://www.davistl.com/services/anodizing_plating.htm

Some pages actually showing the process:
http://www.focuser.com/atm/anodize/anodize.html
http://hem.passagen.se/ballista/anod_eng.html

And one does get the "clear" anodization by using same
process, but not using dye.

There are as well other methods for getting, for example
titanium, colourfull surface films. These are used in
jewelry etc. and some do refer to these as anoziding
(anodic polarization). However, the aluminium anodizing
does as common term refer to the dye process described
above.

Kristian Ukkonen.


From daemon Wed Dec 5 01:13:18 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 05 Dec 2001 08:15:59 +0100
Subject: RE: artifacts and blunders

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Hi

Not sure if this one fits the bill but it is a similar one to the grape
disease story by Maureen Petersen.

The one I mean is the discovery/description of Helicobacter pylori in human
gastric biopsies in the early 80's (when it was given the name
Campylobacter pylori). It had been there all of the time but had been
disregarded as 'stuff'.

I seem to recall a similar story regarding the finding of Actinomyces in
cervical smear material.

We tend to see what we expect might possibly be there and consciously or
unconsciously ignore the rest as 'noise' - perhaps a natural action but
also potentially dangerous.

I have not looked up the original references either but a search on
www.google.com
will provide much information.




Med vänliga hälsningar/With best wishes

Gareth

"Close your eyes and look. What you saw at first is there no more; and what
you will see next has not yet come to life" Leonardo da Vinci (1452-1519).

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734
Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Wed Dec 5 07:48:29 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 05 Dec 2001 08:34:08 -0500
Subject: Re: Al SEM Stubs: is it Anodized or Not....

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We usually tend to avoid issues between suppliers which are
controversial, but based on our long experience in the business in this
area, we shall.
Ladd, being the oldest EM supplier and first company to provide
commercail SEM mounts, has never anodized our mounts. We continue to
machine polish them as we have done thru the years. Based on our long
history we feel that anodizing would be ineffective at best and perhaps
just increase cost unnecessarily.


John Arnott

Disclainer: Ladd Research sells EM supplies, including SEM mounts.
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
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Quality Since 1955





zaluzec-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} As I recall from my metallurgy
}
} To "Anodize" is to subject a metal to an electrolytic action as the
} anode of a cell in order to coat/deposit a protective or decorative film.
}
} Most aluminium anodization that I have seen creates a dark
} THICK oxide film (tens to hundreds of nm thick) and is insulating.
}
} I've never seen this on an aluminium SEM stub. Certainly many I've used are
} electropolished and some (the ones we make here in our machine shop )
} are simply milled and mechanically polished. All will at a minimum
} have a native oxide.
} All are conductive, and yes (Jim) I've seen polished
} (electrochemical, mechanical) as
} well as all ranges of "machine" finished products. These are NOT anodized, they
} are all simply oxidized by leaving them sit in air. The native oxide
} which forms
} is a few tens of angstroms thick and is stable, by definition this is not
} anodization. I have never heard of anyone calling this process "anodization"
} should that be what you are referring to.
}
} So... now I'm curious and I would request that the various suppliers
} who like reply
} to this list to fill us all in on what you do.
}
} 1.) How many of you anodize your stubs (ie. coat), vs electropolish,
} vs mechanically polish.
} 2.) If you intentionally anodize, (ie. deposit/coat) your stub with
} an electrodeposited layer
} how thick is your film, and what do you anodize it with.
} 3.) It might also help to define the "anodization" process (if you do
} this) so that we are all
} understand what each of you mean in the context of how you make the
} stubs we purchase.
}
} Nestor
} Your Friendly Neighborhood SysOp.



--

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Quality Since 1955


From daemon Wed Dec 5 08:06:59 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 05 Dec 2001 08:58:13 -0500
Subject: TEM video rate CCD camera

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers,
I know this topic has come up previously but many of the responses have gone directly to the inquirer rather than to the list. I am looking for information about video rate CCD camera for TEM below $50,000 in cost. The intended use for the camera is teaching and assisting investigators in viewing samples in a service lab situation. It is not meant for capture of high quality images but capture of lower quality images "for the record" would be helpful.
I am already in touch with Gatan concerning such cameras so would like to hear about other options.
I would appreciate hearing from users regarding their recommendations and experiences but do not mind hearing directly from venders.
Thank you,
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Wed Dec 5 08:10:54 2001



From: robert.fowler-at-tdktca.com
Date: Wed, 5 Dec 2001 09:04:06 -0500
Subject: Re: Aluminum Stubs

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Ken,
If a smoother finish is in order then I also use a more aggressive media
(60 - 120 mesh). This media gives a much smoother "glossy" finish but I
personally have never had a reason to have the surface that smooth.

Robert Fowler
Quality Assurance Technician (Failure Analysis)
TDK Components USA, Inc.
Multilayer Ceramic Capacitor Division
1 TDK Boulevard
Peachtree City GA 30269-2051
Telephone: (770) 631-0410 Ext.315
Fax: (770) 487-1460
email: rfowler-at-tdktca.com
www.tdk.com



Ken Converse
{qualityimages-at-n To: "robert.fowler-at-tdktca.com"
etrax.net} -at-sparc5.microscopy.com, "MSA, listserver"
{Microscopy-at-sparc5.microscopy.com}
12/04/2001 05:34 cc:
PM Subject: Re: Aluminum Stubs






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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Robert,
I also bead-blast stubs. Works very well as long as you're not looking
for a smooth surface, then you have to grind and polish afterwards, but
the bead blasting DOES clean that top surface in a hurry. On Al I
usually only blast at 35psi to keep the erosion down.

I have several pieces of Al that have a lot 1/8" holes drilled in them.
Load'em up with stubs and blast away! It's fast, it's fun and
reasonably ecologically sound. Follow with a BRIEF ultrasonic bath in
JOY, rinse with tap, DI or distilled water, depending on how particular
you are, and dry. Alconox and some of the other heavy-duty lab cleaners
have a pH that is far too high for Al, although they work great on glass.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

"robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
} Given that my curiosity is up per the plating/no plating of the stubs,
my
} posting is geared more towards the actual reusing of stubs. For the past
} eight years I have been subjecting a set of 50 or so stubs to a sand
} blasting treatment. You may question the feasibility of a sandblaster BUT
} what is important is the media used to blast with. glass beads in the
range
} of 170-325 mesh are used to effectively remove residue, glue, tape, and
any
} material that resides on the stub. This particular media results in no
} residue and no tolerance change, although a pure water treatment and
} ultrasonic wash are utilized. I have had no trouble at all even after
this
} amount of time.
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}
}
}







From daemon Wed Dec 5 08:22:19 2001



From: Leslie Eibest :      leibest-at-duke.edu
Date: Wed, 5 Dec 2001 09:13:52 -0500
Subject: Re: adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul;

One of my customers needs to remove AFM cantilevers from SEM
stubs after examination without breaking them. Standard SEM
adhesives are too sticky for him too.

We cut a square from the adhesive section of a Post-It note,
and used double-stick tape to attach it to the stub with the Post-It
adhesive facing up. It is sticky enough to hold his small samples in
place - if your samples are not too large or heavy, it might be worth
a try.

Leslie Eibest
SEM lab


From daemon Wed Dec 5 09:24:48 2001



From: Dean Miller :      miller-at-anl.gov
Date: Wed, 05 Dec 2001 08:37:52 -0600
Subject: SBIR program for electron beam instrumentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

The Department of Energy has issued a solicitation for Small Business
Innovative Research (SBIR) proposals related to electron beam
instrumentation. This is a great opportunity for small businesses to apply
for research funding to help develop their creative ideas for
instrumentation and methods that can help advance the field. This is the
second year the solicitation includes electron beam instrumentation and it
is hoped that a strong set of proposals will be received.

The web site detailing the solicitation is:
http://sbir.er.doe.gov/sbir/Solicitations/FY%202002/BES.htm#T9
(This will take you directly to the portion dealing with electron beam
instrumentation.)

Electron beam instrumentation is included in technical topic 9, "NEUTRON AND
ELECTRON BEAM INSTRUMENTATION." Part b covers Electron Beam
Microcharacterization Facilities.

In short, grant applications are sought:

-to develop stages and holders with new capabilities for in situ experiments
in the transmission electron microscope.

-to develop electron sources for scanning transmission electron microscopy
with brightness on the order 109 Amp/cm2/steradian or higher.

-for systems for automated data collection and processing.

-for improved x-ray and electron detectors.

The closing date is Jan 15, 2002 but please be sure to check the web site to
verify this!!

Please distribute this information to your colleagues that may have an
interest in this area and encourage them to think creatively. If there are
any questions, please contact the SBIR program office or you may contact me
off-line.

Best regards,
Dean


---------------------------
Dean J. Miller
Materials Science Division
Argonne National Laboratory
Argonne, IL 60439

630-252-4108 (office)
630-252-7777 (FAX)

miller-at-anl.gov





From daemon Wed Dec 5 09:24:49 2001



From: Richard Beanland :      richard.beanland-at-marconi.com
Date: Wed, 5 Dec 2001 08:39:26 -0600
Subject: TEM Calibration

Contents Retrieved from Microscopy Listserver Archives
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We use cleaved edge specimens of III-V multilayers. We can measure layer
thicknesses in-house to a good accuracy (~0.1%) using high resolution X-ray
diffraction, whereas the mag-i-cal is (I believe) only guaranteed to +/- 2%.
Measurement errors from TEM negatives puts the error in calibration up to
about 0.5%. The thin area on the samples is miniscule and they are very
robust. A drawback is that you need to be able to tilt the sample to 45
degrees. As discussed some time ago on this listserver, you need to be
careful to eliminate lens hysteresis effects if you want to make your
measurements accurate.

Cheers,

Richard Beanland
Marconi Caswell Ltd.
Towcester,
Northants,
NN12 8EQ
UK

e-mail richard.beanland-at-marconi.com


-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: 03 December 2001 18:53
To: MICROSCOPY BB


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John M. Basgen wrote:
===============================================================
We have been using carbon grating replicas for calibrating the magnification
of our TEM images for many years. We are in the process of revising our
imaging and morphometry protocols. I am not happy with the precision and
longevity of the carbon grating replicas. Are there more precise and/or
stronger standards for TEM magnification calibration? The final
magnifications of our images range from 3,000 -20,000 X.
================================================================
Have you seen the MAG*I*CAL(tm) TEM Calibration Specimen as described on the
SPI website, page URL
http://www.2spi.com/catalog/standards/magical.html

It seems to get around most of the problems associated with the carbon
grating replicas and although nothing is forever, it is quite robust in
comparison and should last quite a long time.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


From daemon Wed Dec 5 09:28:03 2001



From: Libby Shaw :      elshaw-at-mit.edu
Date: Wed, 5 Dec 2001 09:35:30 -0500
Subject: Anodizing aluminum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anodizing. Seems like everyone knows what it is,
until they compare notes!

The furor drove me to crack a wonderful older
textbook, Thin Film Processes, edited by John L.
Vossen and Werner Kern, Academic Press 1978.

} From my reading the reason for the confusion is clear:
there really are a lot of ways to anodize aluminum,
some of which result in a clear coating, some colored,
some porous, some hard. The most common and least
expensive method is the sulfuric acid process with
follow-up dying described so excellently on the
web pages Kristian Ukkonen refers us to.

For anyone who is interested, here are some excerpts
on various ways of anodizing aluminum, from the chapter
by Frederick Lowenheim on "Deposition of Inorganic Films
from Solution".

"Although H2SO4 is the most common anodizing electrolyte,
many others are in use. For special effects or attainment
of special properties, chromic acid anodic coatings are
opaque, limited to about 10 um in thickness...

"Phosphoric acid anodizing has been used as a basis for
plating on Al, though it has been largely superseded by
the zincate and other processes.

"Oxalic acid coatings are yellow, and somewhat harder
than conventional H2SO4 coatings. Mixtures of oxalic
and sulfuric acid produce hard coatings, competing with
low-temperature H2SO4 processes.

"Sulfonated organic acids, combined with H2SO4, produce
so-called 'integrally colored' coatings on specific alloys.
Various shades are used in architectural applications...

"Boric acid electrolytes, often with additions of borax,
produce thin barrier oxide coatings used for electrical
capacitors.

"Many anodic coatings on Al can be colored, either with
organic dyes or mineral pigments. Postanodic treatments,
especially that known as sealing, are important in
determining the final properties of the coating..."

Libby Shaw




} From: Kristian Ukkonen {kristian.ukkonen-at-iki.fi}
}
}
} "Paul.Nolan-at-alcan.com"-at-sparc5.microscopy.com wrote:
} } Just to clear up a couple of statements someone made.
} } There is no such thing as a "clear" anodic film.
} } And dies are not used to give anodic films colour ..The colour comes from
} } metals in the anodic layer. Thickness of the layer is also a factor.
} } Paul D. Nolan
}
} The anodizing process for aluminium indeed does require a dye.
} There are plenty of companies selling the dyes etc..
} http://www.caswellplating.com/frames.asp?bottom=/anodizedye.htm
} http://www.plating-supplies.com/Anodizing.html
} http://www.davistl.com/services/anodizing_plating.htm
}
} Some pages actually showing the process:
} http://www.focuser.com/atm/anodize/anodize.html
} http://hem.passagen.se/ballista/anod_eng.html
}
} And one does get the "clear" anodization by using same
} process, but not using dye.
}
} There are as well other methods for getting, for example
} titanium, colourfull surface films. These are used in
} jewelry etc. and some do refer to these as anoziding
} (anodic polarization). However, the aluminium anodizing
} does as common term refer to the dye process described
} above.
}
} Kristian Ukkonen.
}
***************************************************************
Elisabeth L. Shaw, Facility Coordinator
Surface and Spectroscopy Labs
Analytical Shared Experimental Facilities
MIT Center for Materials Science and Engineering

Address: MIT Room 13-4149 Tel: 617-253-5045
77 Massachusetts Ave. Email: elshaw-at-mit.edu
Cambridge, MA 02139 Fax: 617-258-6478
http://web.mit.edu/cmse/www/
***************************************************************


From daemon Wed Dec 5 10:51:05 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 5 Dec 2001 10:44:11 -0600
Subject: Tip flashing: Hitachi S4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

This query is directed specifically at users of the Hitachi S4700 FESEM, but
may also apply more widely to users of FESEM's.

We get a curious result (to me) when flashing the tip. We were advised to
flash until the emission current reading read about 20uA on the "le" meter.
The flash intensity adjustment behind the scope was initially set to
accomplish this in one or two flashes when flashing at an intensity of "2"
in the Setup-Column menu.

That is what happened for quite some time, however for a while now flashing
has resulted in values between 7-12 on the "le" meter. The initial flash
briefly degrades the vacuum at the gun as expected as contamination is
driven off, but subsequent flashes do not, also as expected. However,
subsequent flashes usually LOWER the "le" meter value, rather than raise it
toward our goal of 20. This is the really puzzling part to me. Originally,
the value increased with each flash, and if an excessive number of flashes
were required, we would adjust the intensity pot in the rear of the scope.

We normally run the scope at an emission of 10uA, with kV's of 1.0-10.0.
All vacuum readings are normal, and the scope performs beautifully in terms
of resolution and brightness.

My questions are:

1) Why is the "le" value decreasing with multiple flashes?

2) How dangerous are multiple flashes to the tip?

3) Does the condition described above indicate any problem with the tip
and/or scope?

4) Is flashing actually accomplishing anything if the vacuum gauge shows no
contamination being driven off?

5) Is it safe to keep turning up the intensity pot on the rear of the
scope? (Something tells me no.)

6) Do I simply not understand what's going on? (Something tells me yes.)

Thanks in advance!

Puzzled in Missouri,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Dec 5 10:52:57 2001



From: max.sidorov-at-amd.com
Date: Wed, 5 Dec 2001 08:48:20 -0800
Subject: TEM: ctfExplorer update

Contents Retrieved from Microscopy Listserver Archives
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Dear All:

FYI: ctfexplorer has been updated and it is still free for all.
New features/improvements:
- extended microscope list
- "Euro-bug" removed (problem with Windows locales which use a comma instead of a point for a decimal separator).

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98/NT4/2000.

Please give it a try. Please DO direct your suggestions and comments to sidorov-at-yahoo.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location). Direct link: http://www.maxsidorov.com/ctfexplorer

Enjoy,
__________________________________
Max Sidorov, Ph.D.
sidorov-at-yahoo.com


----------Additional Info----------
ctfExplorer is a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfexplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

Features
- Calculates 1-Dimensional CTF
- Calculates 2-Dimensional CTF
- Calculates Defocus Map
- Calculates point-to-point resolution, Lichte defocus and info limit
- Shows the effects of 2-Fold and 3-Fold astigmatism
- Allows to change Defocus, 2-Fold and 3-Fold astigmatism in real time
- Shows what happens to 1D CTF in different directions when there's astigmatism
- Displays the damping envelopes
- Allows to select a microscope from a list of microscopes
- Allows to create a custom microscope
- Allows to change HT, Cs, Cc, Energy Spread, Convergence for any microscope
- 2 modes of operation: CTF Explorer (to see everything) and CTF Plotter
- Compares 2 microscopes or 2 settings for 1 microscope
- Saves 1D CTF, 2D CTF and "Defocus Map" as bitmaps or metafiles
- Exports 1D plots to tab-delimited text format








From daemon Wed Dec 5 12:02:19 2001



From: Ladd Research :      sales-at-laddresearch.com
Date: Wed, 05 Dec 2001 12:52:20 -0500
Subject: Re: adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

What you are looking for sounds like our product numbers 60550, Double
Coated Aluminum tape. Please contatc me or check our website for more
details.

John Arnott

Disclaimer: Ladd Reserch sells EM supplies. This product may be
available from other vendors.
--

**** Please Note Our New Address, Fax and Phone Numbers ****

LADD RESEARCH
83 Holly Court
Williston, VT 05495 USA

On-line Catalog: http://www.laddresearch.com

TEL: 1-800-451-3406 (US) or 1-802-658-4961 (anywhere)
FAX: 1-802-660-8859
e-mail: sales-at-laddresearch.com

Quality Since 1955

Paul.Nolan-at-alcan.com-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Hello
}
} I'm looking for a double sided adhesive to stick samples (aluminum) onto
} glass slides.
} I want to be able to remove them with relative ease and replace them for
} storage.
} Conductivity is not an issue
} At present i am using those little double sided sticky press down adhesives
} used for SEM stubs ..they are a little to sticky for my liking.
}
} Any suggestions?
}
} Paul D. Nolan
} Electron Optics
}
} Alcan International Limited
} Kingston Research and Development Centre
} P.O.Box 8400, 945 Princess Street
} Kingston, Ontario K7L 5L9
}
} Tel: (613) 541-2066
} Fax: (613) 541-2134
} paul.nolan-at-alcan.com


From daemon Wed Dec 5 13:05:24 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 5 Dec 2001 10:58:05 -0800 (PST)
Subject: Electroscan E3 ESEM problems

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I have another vacuum problem with our Electronscan E3 ESEM after swapping
out a failing rotary pump. The vacuum was working fine and then after
being in standby for a couple days, we went into wet mode and had a vacuum
failure with the message:
"Error cannot Determine state" on the console.
Resetting gives the same message. Power cycling the console gives an "E2
can't pump column error", followed by the cannot determine state message.

Powering everything off and restarting, it seems the diffusion pumps
won't start up now. Everything was working fine, but now I think there is
a problem with the vacuum control unit or a sensor. Is there a way of
resetting the VCU, or some other troubleshooting I can do?
Thanks for any advice.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Wed Dec 5 13:14:49 2001



From: Russell.McConnell-at-tufts.edu
Date: Wed, 05 Dec 2001 15:52:43 -0500 (EST)
Subject: glass fluorescence

Contents Retrieved from Microscopy Listserver Archives
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Thanks for the comment, Mike. Just a bad wording from my side.
MAx

-----Original Message-----
} From: Michael O'Keefe [mailto:MAOKeefe-at-lbl.gov]
Sent: Wednesday, December 05, 2001 10:56 AM
Cc: sidorov-at-yahoo.com


Sorry about the subject name, it is a bit misleading. In using a Leica SP2
confocal, I have noticed that it is possible to see the cover slip and slide.
Why/how is this possible? The setup is essentially this: a coverslip is placed
on a slide, which is then placed on the stage (there is nothing between them,
except of course for miscellaneous small dust particles and air). The slide is
then scanned with light from a HeNe laser (633nm). When the light detection
(wavelengths directed to the PMT's) is set to include 633nm (i.e. 610-650nm), the
signal is of course very bright. I am assuming that this is due to reflection.
What I am confused about is that if the light detection doesn't include 633nm
(sampling from 660-710nm), there is still signal present, and it is in the same
pattern as is seen when looking at the reflected light. It is important to note
that this pattern is connected to the coverslip and slide somehow, as moving the
stage will cause a corresponding change in the image produced by the system.
I realize that I may be missing something fairly obvious, but any help would
be greatly appreciated.

Thanks,
Russell



--
Confocal Imaging Facility Technician
Tufts Medical School
136 Harrison Ave.
Boston, MA 02111
Tel. (617) 636-3795


From daemon Wed Dec 5 17:33:31 2001



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 05 Dec 2001 17:30:57 -0600
Subject: Re: adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie has a great idea. I've used it myself for wafer pieces. Another
thing that I've used is the double-sided carbon tape, but have reduced the
surface adhesion by touching it repeatedly with my fingers or something else
that is strong enough to be pried off without damage. This makes the tape
tacky, but without the death grip is has when it's new.

Leslie Eibest wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Paul;
}
} One of my customers needs to remove AFM cantilevers from SEM
} stubs after examination without breaking them. Standard SEM
} adhesives are too sticky for him too.
}
} We cut a square from the adhesive section of a Post-It note,
} and used double-stick tape to attach it to the stub with the Post-It
} adhesive facing up. It is sticky enough to hold his small samples in
} place - if your samples are not too large or heavy, it might be worth
} a try.
}
} Leslie Eibest
} SEM lab

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
SC Packaging Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Dec 5 17:33:31 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 5 Dec 2001 18:12:42 -0500
Subject: TEM Calibration

Contents Retrieved from Microscopy Listserver Archives
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I have commented on advantages of using the MAG-I-CAL sample in the past and will not do so again here.

I want to comment on Richard's response below. Before I do so, I want to say that I am an overly enthusiastic supporter and believer in the Small Angle Cleavage Technique (SACT) or MicroCleave(TM) Technique and that South Bay Technology is the only company that makes it and I don't work for them. (I hope that this helps keep any feathers from being ruffled.)

Richard, SACT is a marvelous way of preparing III-V samples very quickly and it is an excellent way of calibrating layer thickness values in the TEM when used in conjunction with high resolution X-ray diffraction. I have a PDF file on the SBT web site (https://www.southbaytech.com) that illustrates how this is done. Search for the Microcleave Kit (model number 520) and then look at the application note number 61. The advantage that the SACT method has is that you can get all the thickness that you need from very thin to very thick. By setting up the appropriate imaging conditions, thickness of the layers can be measured very accurately. Similar to what you have said in your response, you can use them to calibrate the TEM. This is what John McCaffrey did when he created the Mag-i-cal sample. BTW, application note #226 is a poster of the technique that John and I put together and is also available on the SBT web site.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Richard Beanland [mailto:richard.beanland-at-marconi.com]
Sent: Wednesday, December 05, 2001 9:39 AM
To: Microscopy-at-sparc5.microscopy.com


We use cleaved edge specimens of III-V multilayers. We can measure layer
thicknesses in-house to a good accuracy (~0.1%) using high resolution X-ray
diffraction, whereas the mag-i-cal is (I believe) only guaranteed to +/- 2%.
Measurement errors from TEM negatives puts the error in calibration up to
about 0.5%. The thin area on the samples is miniscule and they are very
robust. A drawback is that you need to be able to tilt the sample to 45
degrees. As discussed some time ago on this listserver, you need to be
careful to eliminate lens hysteresis effects if you want to make your
measurements accurate.

Cheers,

Richard Beanland
Marconi Caswell Ltd.
Towcester,
Northants,
NN12 8EQ
UK

e-mail richard.beanland-at-marconi.com


-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: 03 December 2001 18:53
To: MICROSCOPY BB


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John M. Basgen wrote:
===============================================================
We have been using carbon grating replicas for calibrating the magnification
of our TEM images for many years. We are in the process of revising our
imaging and morphometry protocols. I am not happy with the precision and
longevity of the carbon grating replicas. Are there more precise and/or
stronger standards for TEM magnification calibration? The final
magnifications of our images range from 3,000 -20,000 X.
================================================================
Have you seen the MAG*I*CAL(tm) TEM Calibration Specimen as described on the
SPI website, page URL
http://www.2spi.com/catalog/standards/magical.html

It seems to get around most of the problems associated with the carbon
grating replicas and although nothing is forever, it is quite robust in
comparison and should last quite a long time.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


From daemon Wed Dec 5 17:36:03 2001



From: microscope-at-tin.it ()
Date: Wed, 5 Dec 2001 17:29:17 -0600
Subject: Ask-A-Microscopist: Koehler method question

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (microscope-at-tin.it) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
December 5, 2001 at 09:25:51
---------------------------------------------------------------------------

Email: microscope-at-tin.it
Name: Massimo

Organization: None

Education: Graduate College

Location: Torino; Italy

Question: 05 December 2001

Let introduce myself. I am an Italian amateur naturalist. I have a
light microscope and I have arranged an iris diaphragm on the
collector lens of illuminator system to performing an illumination
according to Koehler method.
I do not understand well the last procedure step:

{ { Step 4. Now lift the eyepiece out of the body tube and look down
the tube at the back lens of the fully-lighted objective. (This is
best accomplished by the use of a pinhole eyepiece; an eyepiece with
a tiny hole) while looking down the microscope tube, slowly open and
close the substage condenser aperture iris diaphragm. It will be seen
that closing the aperture iris diaphragm "cuts into" the periphery of
the back lens of the objective. For excellent illumination and
contrast, approximately º-1/3 of the back lens should be occluded,
thus leaving 2/3-3/4 of the back lens illuminated. Then replace the
eyepiece in the tube. This step too must be repeated each time a
different objective is turned into place on the nosepiece.} }

So just a few questions:

how much would the hole diameter be of the pinhole eyepiece? And in
which way would I have to use it? Or what would I expect by seeing
throught the hole?

There are some photos or web location where I could see the back lens
to become occluded by closing or opening the aperture iris diaphragm?
And to understand the meaning of this operation.

Thank you

Best Regards

Massimo

%

---------------------------------------------------------------------------


From daemon Wed Dec 5 17:36:44 2001



From: robert.s.mattingly-at-grc.nasa.gov ()
Date: Wed, 5 Dec 2001 17:29:58 -0600
Subject: Ask-A-Microscopist:Adaptor for Nikon/Olympus system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (robert.s.mattingly-at-grc.nasa.gov) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
December 5, 2001 at 14:00:05
---------------------------------------------------------------------------

Email: robert.s.mattingly-at-grc.nasa.gov
Name: Bob Mattingly

Organization: NASA Glenn Research Center

Education: Undergraduate College

Location: Cleveland, Ohio, USA

Question: I have heard of an adapter for mounting a Nikon
995 Digital Camera to an Olympus SZH-ILLD
Macroscope. I cannot find any information on the
manufacturer of an adapter to meet my application.
Are you aware of any suppliers?
Thanks in advance for your help with this issue.

---------------------------------------------------------------------------


From daemon Wed Dec 5 17:55:47 2001



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 5 Dec 2001 17:50:07 -0600
Subject: LM: Leica Orthoplan 2 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our Leica Orthoplan 2 needs to be decontaminated. Fungi have grown on
the front surface mirrors in the internal prism of the main stand.

In the past, we had to return the stand to Germany for repair but are
wondering if anyone knows of a qualified Leica repair center in the
US. Barring this, we would purchase a second Orthoplan 2 main stand
if one were available.

Thank you.

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################


From daemon Wed Dec 5 19:26:09 2001



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 05 Dec 2001 20:20:02 -0500
Subject: Re: Electroscan E3 ESEM problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is a while since our E3 departed, so my memory of the symptoms is a bit
rusty. However, a problem we had from time to time was the "U" bend in the
vibration isolation block in the pumping lines filling with oil, and
thereby preventing any pumping from taking place, once the column has been
pumped to a modest vacuum. This would cause the vacuum pumps to fail to
start as you described.

The remedy was to disconnect the rubber hoses, pour out the oil, then
reassemble and start up.

I do recall seeing the error messages you describe, but whether they were
associated with this particular fault I can't now remember.

Tony Garratt-Reed.

At 10:58 AM 12/5/2001 -0800, Gordon Vrololjak wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Dec 5 21:33:59 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 05 Dec 2001 19:33:39 -0800
Subject: Re: Ask-A-Microscopist:Adaptor for Nikon/Olympus system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Optem at http://www.optem.com

They make nice adapters for the 990 to most any scope.

gary g.



At 03:29 PM 12/5/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Dec 5 22:18:55 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 05 Dec 2001 20:20:35 -0800
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to this query. There is much
more to this than meets the eye. Rather typical in this field
it seems.

Finding a sharp edge seems easy enough. Not so. Sharp is
sharp but really sharp is hard to find. At 200KX, sharp is not
so sharp. I used some xxxx maker's apertures only to find
that they were not so sharply defined. I tried two others and
found that they too were not sharp. I finally found one of my
Amray 70u Au coated apertures to be sharp enough to use.
I had to tilt it to make a truly sharp edge. The trick is to
increase contrast, decrease brightness and slow scan the
image at 200KX. Then, do the line scan. I go the following
results:

10KV, CL0, WD4mm spot size=168A
10KV, CL5, WD4mm spot size=129A

Do these figures make sense?

With my Amray FESEM, higher CL# means smaller spot size.
So the spot size numbers seem to jive.

This is no small feat to measure!! The key is to do the slow
scan first and then do the line scan. Otherwise, there is too
much mechanical noise and human interference (coughing)
which corrupts the results.

I'm going to do CL15 and CL20 tomorrow and see what I get.

I intuitively see the rationale for how this works. It becomes
more obvious as I work with it. The Gaussian aspects are
surprising yet rather logical. A 3D plot of a spot would surely
look like this. -- good stuff.

A recent example image is at http://photoweb.net/spot-23.tif


Thanks.

gary g.


At 03:53 PM 11/26/2001, you wrote:
} The SEM spot size can be measured by doing a line scan across a sharp edge.
} For more details see the SEM lab workbook by Lyman et al., 1990.
}
} Krassimir Bozhilov.
}
}
} }
} } Hi all:
} }
} } Is there any direct way to quantitatively determine
} } and measure the actual spot size of a SEM beam?
} }
} } Given various KV, WD, final aperture sizes, condenser lens
} } settings, etc., is there a way to truly find out what
} } the real spot size is on the specimen?
} }
} } Any ideas?
} }
} } gary g.
} }
} }



From daemon Thu Dec 6 02:03:53 2001



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 5 Dec 2001 21:55:59 -1000 (HST)
Subject: Re: Artifacts and blunders (Long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Alan and Listers-
}
} I am interested in gaining insight into the role of microscopic artifacts
} in the history of biology.


Although I 'm sure there are examples of misinterpretation of artifacts
that have been perpetuated for many years, I can offer here a story of the
flip side. This is about what appeared to be a well-known artifact that
turned out to be, in fact, a surprising real structure. It's also an
embarrassing personal story!

An undergraduate student was working with some colleagues who were
studying the behavior and neurophysiology of the escape response of
several species of planktonic copepods (small crustaceans). They had reams
of data and were beginning to characterize the responses of the animals to
different kinds of stimuli. Each species had it's own characteristic
response, but a general picture was beginning to form. There appeared to
be two different classes of response though, one type particularly
puzzling. The undergrad spent some time in the EM facility with me, using
SEM to describe the sensory setae. She expressed an interest in trying TEM
and so we went through fixation, embedding, sectioning, etc. I had worked
on the tiny beasts for close to 10 years and had found some interesting
structures that I worked on from time to time. Copepods were difficult to
fix, some species more so than others. So I had concentrated on the
"pretty" ones and temporarily given up on the "ugly" ones - the ones with
all those myelin body artifacts that I just couldn't seem to
avoid. Finally it was time to try to work on them again, so I gave this
one particularly difficult species to the undergrad to try to fix and
section. She came to me with micrographs, wondering what in the world were
those squiggles she saw. I patiently explained that myelin bodies were a
fixation artifact, produced when lipids became mobile during fixation,
then reformed in these "onion bodies" . I sent her back to work to try
again, and she came up with the same results. "What if they aren't an
artifact?" she kept asking. I explained that invertebrates do not have
such membranous structures; they were artifactual. I brought out the books
and papers about myelin body artifacts. I brought out the books and papers
that said invertebrates do not have membranous wrappings around their
axons. She was unconvinced. In a hurry one day when she was really bugging
me I dug through my files and found the decade-old folder to show her I'd
seen the same thing - an artifact. "But there's an axon in the middle of
each one", she protested, not knowing that was "impossible". I glanced at
them impatiently and saw that she was right, but she was running off to
class.

Later that day we looked closely and decided that, indeed, the images
were a real mess and difficult to interpret, but there was a faint
possibility that these weren't classic myelin body artifacts. Whatever it
was was certainly reproducible, and these forms appeared every time in the
antennal nerve. So we emailed the PIs of the project and told them we
might have myelinated axons in the difficult-to-interpret species. It was
April1, April Fools' Day, the undergrad's name was April, and they thought
it was a joke. After all, the dogma is that invertebrates don't have
myelin! Check any biology book.

But by the next day they realized that myelin (OK, "myelin-like
structures" for you purists) would instantly and completely explain 10
years' worth of puzzling data.

It took awhile to prove to ourselves (mainly me, since I'd been repeating
the dogma for many years) that these structures were real, orderly, and
extensive wrappings of membrane around the axons. It took
ultrarapid cryofixation and cryosubstitution to really show that the
artifactual bodies that had plagued a number of people working on these
critters were in truth these surprising, dogma-busting structures.

We made the April the first author on the Nature paper, which came out in
April 1999.

I don't take anything for granted anymore! The lesson is: keep an open
mind.

For more on the copepod story- Copepod neuroecology
http://www.pbrc.hawaii.edu/~petra/copepod.html


Aloha,
Tina

http://www.pbrc.hawaii.edu/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Dec 6 03:05:27 2001



From: Mustapha Jouiad :      jouiad-at-lmpm.ensma.fr
Date: Thu, 06 Dec 2001 09:54:07 +0100
Subject: TEM specimen thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi There,
I'm interested in mesuring specimen thickness in TEM. In my case these two
techniques (covergent beam technique and the spacing Moire franges) can not be
applied. I'll apreciate any further suggestion.
Thanks in advance
M. Jouiad

--------------------------------------------------------
DR. Mustapha JOUIAD

ENSMA/LMPM
Teleport 2, 1 Ave. Clément Ader, Futuroscope-Chasseneuil 86960
Tel. 33 (0) 5 49 49 82 09
Fax. 33 (0) 5 49 49 82 38
Email. jouiad-at-lmpm.ensma.fr
Web. www.lmpm.ensma.fr
[]
--------------------------------------------------------


From daemon Thu Dec 6 04:59:02 2001



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Thu, 6 Dec 2001 11:57:41 +0100
Subject: SEM: probecurrentmeter?

Contents Retrieved from Microscopy Listserver Archives
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Hi There,

.. does anbody have an idea (... or even better plans) how to build a more or less
"homebrewn" probecurrentmeter (... FEGSEM -} pA resolution needed) ... a comercial
instrument is probably to expensive??? (} = 10TDM,- = 5000€,- ???)
.. i just had to learn that this is a very useful tool!!!


Best regards!
Gunnar



Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195 379391
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the
position of the MPI-P or the MPG!



From daemon Thu Dec 6 07:40:29 2001



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Thu, 6 Dec 2001 07:30:40 -0600
Subject: re: TEM specimen thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi, Mustapha,
you could try EELS for that; the method can give you a very precise value for
local thickness.

Corneliu Sarbu, PhD
MTMDept.
Belgium

- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi There,
I'm interested in mesuring specimen thickness in TEM. In my case these two
techniques (covergent beam technique and the spacing Moire franges) can not be
applied. I'll apreciate any further suggestion.
Thanks in advance
M. Jouiad

--------------------------------------------------------
DR. Mustapha JOUIAD

ENSMA/LMPM
Teleport 2, 1 Ave. Clément Ader, Futuroscope-Chasseneuil 86960
Tel. 33 (0) 5 49 49 82 09
Fax. 33 (0) 5 49 49 82 38
Email. jouiad-at-lmpm.ensma.fr
Web. www.lmpm.ensma.fr
[]
--------------------------------------------------------


- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -


From daemon Thu Dec 6 09:29:11 2001



From: Ken Bart :      kbart-at-hamilton.edu
Date: Thu, 6 Dec 2001 10:15:50 -0500
Subject: Flatbed Scanner Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to everyone who responded to my request for information about
flatbed scanners! There appears to be a number of very good
instruments out there. Thanks for your suggestions!

Ken
--


Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323

Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu
http://academics.hamilton.edu/biology/kbart/


From daemon Thu Dec 6 11:41:24 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 06 Dec 2001 09:39:20 -0800
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would not think that specimen current is independent of
spot size. In fact, I go to great lengths to adjust KV, WD and
spot size to obtain just the right specimen current to image
upper metal on microchips underneath 10000A of SiN. I lay
down a Schottky barrier for the particular type of passivation
and adjust the SEM parameters for best picture. Too high of
specimen current produces too many SE and thus charging and
bad images. The optimum is just enough SE for detail and plenty
of BSE for topography.

I can measure specimen current using my Fluke DMM. If the value
is not too low, the Fluke will read it (nA). I also use it to measure
gun brightness as part of routine calibration.

I'll try measuring the currents today and see what gives.

gary g.


At 06:00 AM 12/6/2001, you wrote:
} Hi
}
} I am very interested with this topic, and we have done such mesures years
} ago, as we buid our lab-made LEED optics, with low energy guns, but with
} spot sizes of microns... I have for our Jeol 840 a table buid with
} such mesures by an japanese ingenior of Jeol Europe, which gives the spot
} size versus WD, Energy, and current. So for exemple, with 10 keV, 10E-11A
} and 8 mm WD, it should be 110 angstrom. It's a w type gun. This table is
} of course not valuable for an other SEM, and I'll have to built it for our
} FE-SEM...
}
} So, a question : do you have a mesure of the probe current (faraday cup,
} or as sample current on carbon tape), corresponding to the condenser
} settings you have used ? In case our FE-SEM (Jeol 6700F), Jeol claims that
} the spot size is independant of the probe current, and I want to verify
} it. And comparaison with an other SEM is interesting too.
}
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Dec 6 12:13:55 2001



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Thu, 06 Dec 2001 13:17:37 -0600
Subject: specimen thickness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





I would like to add a comment to John's discussion on calibration samples. I used both the diffraction gratings and the latex spheres prior to John's development of the Mag-i-cal sample. I found that over the overlapping magnification range that both were suitable, they did not agree. Further, I did see changes in the latex spheres at long exposures --I believe it was when I used a lower energy microscope.

I had to do periodic QS9000 (automotive industry equivalent to ISO-9000) calibration procedures for the microscope. With the Mag-i-cal sample, all of the microscope calibrations can be done with one sample very quickly. It is the only way to go.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: John McCaffrey [mailto:john.mccaffrey-at-nrc.ca]
Sent: Thursday, December 06, 2001 1:07 PM
To: Microscopy listserver





You can also determine specimen thickness with electron energy loss
spectroscopy
(EELS) by applying the log-ratio technique to the low loss spectra (see
Egerton,
Electron energy loss spectroscopy in the electron microscope, New York:
Plenum,
1996). This technique yields a result in units of the mean free path
for
inelastic scattering.


Mustapha Jouiad wrote:

} Hi There,
} I'm interested in mesuring specimen thickness in TEM. In my case these
two
} techniques (covergent beam technique and the spacing Moire franges)
can not be
} applied. I'll apreciate any further suggestion.
} Thanks in advance
} M. Jouiad
}
}

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Thu Dec 6 14:47:34 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 06 Dec 2001 12:47:02 -0800
Subject: Re: SEM spot size

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I have completed many measurements of spot size and specimen
current. Tabular data can be found at http://photoweb.net/SEMLog.pdf

This SEM is a Schottky field emission scope. One of the routine
periodic checks is to measure the gun brightness. The spec is 12.5nA.
This gun is doing 13.81nA and is just a bit higher than the +-1.5nA
limits. The method of correcting this is to adjust the extractor voltage
by 100 Volt increments and see how the brightness changes after about
an hour. Then, once stable, that is the new extractor voltage. Extractor
current can vary greatly whereas the gun brightness is quite steady.

Gun brightness is measured with a 4-digit Fluke 79-III DMM connected
to the stage alarm BNC jack. The beam is directed into a Faraday cup
located on the stage, CL is opened up to -35 and mag is set to 150KX.
The voltage reading on the DMM divided by 1E7 (10 Meg Ohms) is
the current at the Faraday cup. I used this same method for measuring
specimen current with the target aperture for measuring spot size.

The numbers are somewhat believable. However, at low CL values,
the specimen current became negative. So I suspect that this method
does not work for really low values of specimen currents.

Can anyone comment on the sanity level of the spot size values
I have derived? Are these reasonable for a FESEM at 10KV, 4mmWD,
and 100u final aperture?

tnx,
gary g.



At 06:00 AM 12/6/2001, you wrote:
} Hi
}
} I am very interested with this topic, and we have done such mesures years
} ago, as we buid our lab-made LEED optics, with low energy guns, but with
} spot sizes of microns... I have for our Jeol 840 a table buid with
} such mesures by an japanese ingenior of Jeol Europe, which gives the spot
} size versus WD, Energy, and current. So for exemple, with 10 keV, 10E-11A
} and 8 mm WD, it should be 110 angstrom. It's a w type gun. This table is
} of course not valuable for an other SEM, and I'll have to built it for our
} FE-SEM...
}
} So, a question : do you have a mesure of the probe current (faraday cup,
} or as sample current on carbon tape), corresponding to the condenser
} settings you have used ? In case our FE-SEM (Jeol 6700F), Jeol claims that
} the spot size is independant of the probe current, and I want to verify
} it. And comparaison with an other SEM is interesting too.
}
}
} J. Faerber
} IPCMS-GSI
} (Institut de Physique et Chimie des Matériaux de Strasbourg
} Groupe Surface et Interfaces)
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr



From daemon Thu Dec 6 16:43:17 2001



From: ebhan-at-cybermed.ucsd.edu
Date: Thu, 06 Dec 2001 14:32:20 -0800
Subject: Olympus IMT-2 filters

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Hi all,

I have an old Olympus IMT-2 inverted scope. It has sliders for inserting
filters into the light path for fluorescence imaging. Right now I can only
image two fluorophores limited by my slider slots. Does anyone know of
some way I can image three dyes, i.e. a filter cube or expanded slider
system, specifically for the IMT-2?

Thanks for your help!

Ed Han


From daemon Fri Dec 7 04:25:32 2001



From: Richard Beanland :      richard.beanland-at-marconi.com
Date: Fri, 7 Dec 2001 10:13:58 -0000
Subject: Fw: TEM Calibration

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John,
I'd just like to add a couple of points - I'd like to make it clear that I
do think that the the mag-i-cal specimen is probably the best commercially
available calibration sample. I didn't mean to be disparaging! It's much
better than diffraction grating replicas or other commercially available
calibration specimens - it beats diffraction grating replicas in that you
can use the crystallography to eliminate specimen tilting and is also more
stable. Also, both this and the SACT technique have the advantage that you
don't need large tilts and could be used in a single tilt holder.

The extent to which you calibrate your microscope depends on what you use
it for - you can get a very accurate relative calibration by taking pictures
of the same region at different magnifications. You are only then limited
by how accurately you can measure features on the negative (0.05 mm in 5cm
is readily achievable with the right specimen and a lupe, which gives you 1%
relative accuracy). Once you get down to layers below 100 atomic layers
thick then of course it becomes impossible to get better than 1% accuracy
with HRTEM. Lack of contrast between the different layers can make things
even worse at larger thicknesses for some samples.

The 0.1% layer thickness accuracy I quoted was for X-ray diffraction, and
for the right sample XRD does beat TEM every time in terms of accuracy.
However, most device structures can't be measured using superlattice fringes
in XRD and that is where TEM comes in. Of course we're only measuring
thickness because it is a measure of device performance; really thin layers,
below 100 atomic planes thick, are usually only used as quantum well
structures, delta-doping layers or etch stops, the effectiveness of which
can usually be assessed by means more relevant to the device performance
than layer thickness (e.g. PL, sheet carrier density, overetching). (I am
unaware of any applications which need high accuracy from thin layers apart
from microelectronics(?))

I do XRD with a 1mm spot on the sample I use for calibration and make a
cleaved edge specimen from a region as close as possible to that position.
With a 1% thickness variation over a 3" wafer I'm happy that this is truly
representative. With lattice matched III-V layers you can grow as thick as
you like, and you get better contrast in dark field 002 images. The
downside is that the samples are less stable chemically and physically than
Si/SiGe which doesn't lend itself to commercial supply (I certainly wouldn't
like to put 100 InP cross sections in the post with a bet that more than 50%
would arrive unscathed).


Richard Beanland
Marconi Caswell Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK

e-mail richard.beanland-at-marconi.com


-----Original Message-----
} From: John McCaffrey [mailto:john.mccaffrey-at-nrc.ca]
Sent: 06 December 2001 18:07
To: Microscopy listserver

Hello John,

While I noted that you weren't too happy with the replica
samples, these calibration samples (particularly the cross grating replica
samples) are probably still the best choice for the calibration ranges in
which you are working. A common cross-grating replica (see, for example,
http://www.2spi.com/catalog/standards/othertem10.html) has a line density of
2160 lines/mm, which would give you a line spacing on your negatives of 1.39
mm -at- 3000X and 9.26 mm -at- 20,000X. This length would allow you to measure
tens of spacings at all of your magnification ranges, and cut your
uncertainty down to reasonable levels. While the edge definition of the
grating replicas is not the greatest, and while you may have to replace the
sample every year or two, these calibration samples are still among the most
simple to use, as well as being reliable and reproducible. They are also
inexpensive. Other options include latex spheres, which are in the size
range that would be useful for your required magnification ranges. The
down-side of these samples is that the spheres have a stated average value,
so measurements of many spheres are required to lower the uncertainty to the
desired level. Also, there is some anecdotal evidence that these spheres
change size when exposed to higher electron doses.
If your requirement included the entire set of magnification
ranges of your TEM, the MAG*I*CAL sample is still the best bet.
(Disclaimer: I "invented" the sample, but will attempt to stay objective
about it's merits!). Since this sample and all other samples based on
crystal lattice spacings rely on fundamental constants of nature, they are
the most accurate and precise samples currently available. However, in
spite of this glowing pedigree, crystal-based calibration samples still
cannot give measurements with better than ~1% uncertainty. The response to
your enquiry from Richard Beanland claiming 0.1% accuracy for cleaved III-V
multilayers is a bit misleading (not to mention that he disparagingly
compared this supposed superior uncertainty to my beloved MAG*I*CAL!). His
layer thickness confidence is based on X-ray diffraction (XRD) measurements.
XRD gives very accurate layer thickness measurements, but these measurements
are averaged over enormous surface areas in TEM terms - the diameter of the
x-ray beam, which can be significant fractions of a square mm. This is a
very useful measurement for a layer thickness, but this does not translate
directly into uncertainty in a TEM. When a semiconductor multiplayer is
viewed in cross section, there are a series of contributors to the layer
measurement uncertainty:
The state-of-the-art TEM's have resolutions of approximately 0.2 nm. The
(002) lattice spacings in most semiconductors (the atomic layers parallel to
the surface in most wafers) are a little below 0.3 nm. This means that any
measurement of a thicker semiconductor layer in a TEM is going to have a
"TEM uncertainty" of approximately one atomic layer at each interface.
Producing a semiconductor crystal interface that is perfectly, atomically
abrupt (even over the relatively small volume of material used in high
resolution TEM) is not trivial, and rarely claimed. Any additional atoms
from layer A mixed into layer B, or vice versa, will tend to blur the
interface when viewed in cross-sectional TEM. This "epitaxial layer"
uncertainty is also approximately one atomic layer at each interface.
Highly competent crystal growers can minimize, but not eliminate this
interfacial intermixing, allowing the combination of the "TEM uncertainty"
and the "epitaxial uncertainty" into one atomic layer at each interface.
Semiconductor multilayers can only be grown up to a certain critical
thickness, at which point the differences in lattice parameter between the
adjoining layers becomes so great that the crystal 'relaxes' with the
generation of misfit dislocations at the interface. These dislocations need
to be avoided in a calibration sample, but the contrasting layer needs to be
grown as thick as possible to minimize the influence (i.e., the percentage)
of the two points above.
Over an entire semiconductor wafer, there will be systematic thickness
variations; i.e., layers may be slightly thicker in the middle of the wafer
than at the edge. This is a fairly small, but still significant variation -
typically less than 1%. Therefore, it becomes important to know where on
the wafer your piece of material comes from.
So, why can't the "God-traceable" crystal-based TEM calibration samples give
measurements with better than ~1% uncertainty? From the combination of
uncertainties given above! The thinnest SiGe marker layers in the MAG*I*CAL
sample are approximately 10.0 nm thick, and considering their two interfaces
to be uncertain in the worse case to approximately one atomic spacing each,
this interfacial contribution to uncertainty is about 0.6%. Coupled with
the {1% uncertainty across the entire MAG*I*CAL wafer gives a total
uncertainty of ~2%, with a safety margin built in. Richard Beanland did
correctly imply the important point that, if one wishes to internally
calibrate one's own sample against a known lattice spacing on that same
sample, the uncertainties will indeed be less. However, 1% uncertainty is a
better estimate of the 'best' uncertainty available in a TEM calibration
sample, even for a sample based on a fundamental constant of nature.
Cheers
John


John P. McCaffrey, Ph.D.
Institute for National Measurement Standards
National Research Council of Canada
M-35 Montreal Road Labs
Ottawa, Ontario
K1A 0R6 CANADA

Tel: (613) 993-2715
Fax: (613) 952-9865
Email: john.mccaffrey-at-nrc.ca



Richard Beanland wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We use cleaved edge specimens of III-V multilayers. We can measure layer
} thicknesses in-house to a good accuracy (~0.1%) using high resolution
X-ray
} diffraction, whereas the mag-i-cal is (I believe) only guaranteed to +/-
2%.
} Measurement errors from TEM negatives puts the error in calibration up to
} about 0.5%. The thin area on the samples is miniscule and they are very
} robust. A drawback is that you need to be able to tilt the sample to 45
} degrees. As discussed some time ago on this listserver, you need to be
} careful to eliminate lens hysteresis effects if you want to make your
} measurements accurate.
}
} Cheers,
}
} Richard Beanland
} Marconi Caswell Ltd.
} Towcester,
} Northants,
} NN12 8EQ
} UK
}
} e-mail richard.beanland-at-marconi.com
}
} -----Original Message-----
} } From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
} Sent: 03 December 2001 18:53
} To: MICROSCOPY BB
} Subject: TEM Calibration
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} John M. Basgen wrote:
} ===============================================================
} We have been using carbon grating replicas for calibrating the
magnification
} of our TEM images for many years. We are in the process of revising our
} imaging and morphometry protocols. I am not happy with the precision and
} longevity of the carbon grating replicas. Are there more precise and/or
} stronger standards for TEM magnification calibration? The final
} magnifications of our images range from 3,000 -20,000 X.
} ================================================================
} Have you seen the MAG*I*CAL(tm) TEM Calibration Specimen as described on
the
} SPI website, page URL
} http://www.2spi.com/catalog/standards/magical.html
}
} It seems to get around most of the problems associated with the carbon
} grating replicas and although nothing is forever, it is quite robust in
} comparison and should last quite a long time.
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================



From daemon Fri Dec 7 04:33:40 2001



From: Richard Beanland :      richard.beanland-at-marconi.com
Date: Fri, 7 Dec 2001 10:26:17 -0000
Subject: Looking of a 'Nanospec' microspectrophotometer or similar in the

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Hi All,
I am attempting to make very accurate measurements of optical
coating thicknesses ( {1%), sampling regions a couple of microns or less in
size. This isn't possible with SEM or TEM due to interfacial mixing and/or
lack of contrast between the layers. (No matter how well calibrated the
microscope may be!) One tool which may do the job (and be more relevant to
device performance) is a microspectrophotometer such as a 'Nanospec' - or
possibly a spectroscopic ellipsometer with very small spot size.
Is anyone aware of such a tool I could try in the UK (I am unable to raise
the suppliers of Nanospec tools). Alternatively is there another
(preferably optical) technique I could use which could also give relevant
data?

Mnay thanks for any ideas you may have,

Richard Beanland,
Marconi Caswell Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK

e-mail richard.beanland-at-marconi.com


From daemon Fri Dec 7 05:46:22 2001



From: Jean Louis HEITZ :      jl.heitz-at-critt.fr
Date: Fri, 7 Dec 2001 12:39:28 +0100
Subject: RE:SEM probecurrentmeter

Contents Retrieved from Microscopy Listserver Archives
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I am about purchasing a picoammeter.

I have found 2 models :

- KE development www.kedev.com
range approx 1 pA - 2 µA
price approx 1200 €

- Keithley www.keithley.com
range approx 0.02 pA - 20 mA
price approx 2000 € (model 6485)

sincerely,

Jean Louis HEITZ

CRITT Matériaux-LNE Est
19 rue de Saint Junien, BP 23
F - 67305 SCHILTIGHEIM CEDEX
tel. +33(0) 388 191 510
fax +33(0) 388 191 514
jl.heitz-at-critt.fr
www.critt.fr



From daemon Fri Dec 7 07:11:05 2001



From: ATC SEM Laboratory :      atcsem-at-earthlink.net
Date: Fri, 7 Dec 2001 08:08:56 -0500
Subject: EDS depth of penetration

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Hello,
I have been dealing with SEM/EDS for only 1.5 years and I have a lot to
learn, but recently I have been asked a question: What is the EDS depth of
penetration on my unit? I work with Amray 1645 and it was upgraded by PGT
for imaging and EDS. Could anybody answer the question How deep does the
x-ray beam penetrates through a sample and, if it is varies, how to find out
actual depth?

Thank you in advance.

Pavel
atcsem-at-earthlink.com





From daemon Fri Dec 7 07:24:46 2001



From: JHoffpa464-at-aol.com
Date: Fri, 07 Dec 2001 08:18:49 EST
Subject: negative staining

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hi all, i am doing collagen extractions from corneas and negative staing them to get the lenghts. my problem is that i am having trouble getting the collagen to stick to the formvar. any suggestions. i am on a deadline to work out the details of this project.
John


From daemon Fri Dec 7 07:48:24 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Fri, 7 Dec 2001 07:41:57 -0600
Subject: Oil backstreaming into SEM chamber

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Dear Listers,

I have an older SEM which over a period of several months seems to
repeatedly develop a small droplet of oil on the EDS detector collimator.

It is a diffusion pumped system. The mechanical backing pump is regularly
serviced and gets oil changes.

I am inclined to think it is time to clean the diffusion pump and change the
oil there. Perhaps over time backstreaming from the mechanical pump has
occurred, causing contamination of the diffusion pump oil with mechanical
pump oil. The latter, which has higher vapor pressure, is evaporating out
of the diffusion pump into the chamber and condensing on the coldest point
there (the detector).

Any opinions on whether this is reasonable or not? Any other possibilities
anyone can think of? I'd hate to change the diffusion pump oil, which is
going to be a big pain, if this is not going to do the trick.

By the way, I'm already planning to install a foreline trap (there currently
isn't one).

Thanks,

Wharton

**********************************************************
Wharton Sinkler
UOP LLC
Des Plaines, IL


From daemon Fri Dec 7 08:24:52 2001



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Fri, 7 Dec 2001 08:15:25 -0600
Subject: Re: glass fluorescence

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Russell,

If the pattern is the same, the signal is probably still reflected
light. This signal can be very large compared to fluorescence and it
is just blasting through (or around) the filters/prism.

Jim Pawley


} ------------------------------------------------------------------------
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****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Fri Dec 7 09:02:21 2001



From: robert.fowler-at-tdktca.com
Date: Fri, 7 Dec 2001 09:58:34 -0500
Subject: Aluminum Stubs - Long Version

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----- Forwarded by Robert Fowler/TCU/TDK-US on 12/07/2001 09:54 AM -----

"Monson,
Frederick C." To: "'robert.fowler-at-tdktca.com'"
{fmonson-at-wcupa {robert.fowler-at-tdktca.com}
.edu} cc:
Subject: RE: Aluminum Stubs - Long Version
12/07/2001
09:48 AM






Thanks for pointing the direction. The only sand blasting I have any
recollection of was many years ago when you could take your spark plugs to
Pep Boys and use a machine they had for public use. Like the fluoroscope
in
Buster Brown(?), they too have disappeared.

Thanks again Bob, I appreciate your time and consideration,

Regards,

Fred


} ----------
} From: robert.fowler-at-tdktca.com
} Sent: Friday, December 7, 2001 9:03 AM
} To: Monson, Frederick C.
} Subject: RE: Aluminum Stubs - Long Version
}
}
} Hello again Mr Monson,
} In the past you have taken the time to help me with a problem on my SEM
} so
} I will take the time to fill you in on the details of my procedures. Due
} to
} liability reasons I have to say that these are my own procedures and I
} cannot endorse anyone using these (reference only). All materials
required
} are readily available from McMASTER-CARR WWW.MCMASTER.COM
}
} P/N
} Price
} 1- Self Contained Vacuum 9808T44 450.00
} 2- HEPA Filter 9808T47 89.00
} 3- Blasting Cabinet 33005K71 250.00
} 4-Media (170-325 mesh) 3386K75 37.00
} 5-Media (60-120 mesh) 3386K72 35.00
}
} Stubs are placed in an appropriate holder. This will vary slightly with
} the
} style of stubs used. A piece of 12 inch square of aluminum with 1/8" or
} 1/4" holes is fine. This is placed in the blasting cabinet. Vacuum turned
} on. Air supplied is usually run at 35 PSI. If residue is thick (most of
} the
} time some tapes such as double sided carbon tape can be mostly removed
} before blasting) then a higher (50 PSI) air pressure is required. Parts
} are
} blasted until visibly clean. 170-325 mesh media will produce a matte
} finish. 60-120 mesh grit will produce a glossier finish and removes
} residues faster. After blasting parts are removed and cleaned in
} ultrasonic
} wash using DI water. Distilled water is fine. This wash is repeated three
} times. The following are clips from correspondence about cleaning stubs.
}
} Robert,
} I also bead-blast stubs. Works very well as long as you're not looking
} for a smooth surface, then you have to grind and polish afterwards, but
} the bead blasting DOES clean that top surface in a hurry. On Al I
} usually only blast at 35psi to keep the erosion down.
}
} I have several pieces of Al that have a lot 1/8" holes drilled in them.
} Load'em up with stubs and blast away! It's fast, it's fun and
} reasonably ecologically sound. Follow with a BRIEF ultrasonic bath in
} JOY, rinse with tap, DI or distilled water, depending on how particular
} you are, and dry. Alconox and some of the other heavy-duty lab cleaners
} have a pH that is far too high for Al, although they work great on glass.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Hi Robert,
}
} You are quite correct, this is undoubtedly the "best" way to clean sample
} mounts for reuse. However, we have had several concerns, and that is why
} we
} have not recommended it ourselves when people ask:
}
} a) In a large central facility, some of the remains of samples could
} contain hazardous materials which would then end up contaminating the
} media
} and in the end, while you have clean sEM mounts, there is created a large
} volume of hazardous waste, and
}
} b) How would you really know that the "cleaned" mounts were really
} "clean"
} and in a virgin pristine state? In other words, what would be the
"test"?
}
} I would be curiously as to how you approach those two issues. We
} ourselves
} have to think about liability issues, and that is why we have not
} aggressive
} promoted this as a means for people to clean and reuse their "used" SEM
} mounts. WE even considered offering a recycling service, and doing mount
} cleaning here, but I did not know how to protect the health and safety of
} my
} people when handing the waste mounts from other laboratories.
}
} Chuck
}
} PS: Please remember that we are nearly 100% paperless and we would ask
} that any reply to this message be by way of the "reply" feature on your
} software, so that the entire string of correspondence can come back to us
} and all be in one place.
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}
} Hello Mr Garber
} In response to your e-mail
} a) The actual blasting unit that I use is relatively small in size. (2
ft
} square) The volume of media used is around 3 quarts. In the eight years I
} have been cleaning stubs the media has been changed maybe twice. This
} material is treated as hazardous waste and disposed of accordingly. In my
} opinion this is a small amount to dispose. Of course the relatively small
} amount of stubs cleaned weekly (50 pcs) has a direct impact on media
life.
} I am not sure of most facilities volume, but if handled correctly
} (dedicated blasting unit) this should not create problems.
} b) That is a very good question. I honestly do not know of a procedure
to
} test to confirm the stubs are actually "clean"
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Many thanks for sharing that information with us. I am sure some of my
} other
} people will find it interesting. As I think I indicated, we have long
} thought of a mechanism for "recycling" used mounts for people (many just
} throw them out) but these other considerations have generally stopped us
} dead in our tracks. But within a given laboratory, like yours, you would
} pretty much know what was or was not on the mounts, and therefore would
} know
} how to properly protect yourself from exposure to bad things.
}
}
}
} Robert Fowler
} Quality Assurance Technician (Failure Analysis)
} TDK Components USA, Inc.
} Multilayer Ceramic Capacitor Division
} 1 TDK Boulevard
} Peachtree City GA 30269-2051
} Telephone: (770) 631-0410 Ext.315
} Fax: (770) 487-1460
} email: rfowler-at-tdktca.com
} www.tdk.com
}
}
}
}
} "Monson,
}
} Frederick C." To:
} "'robert.fowler-at-tdktca.com'"
} {fmonson-at-wcupa {robert.fowler-at-tdktca.com}
}
} .edu} cc:
}
} Subject: RE: Aluminum Stubs
}
} 12/05/2001
}
} 10:38 AM
}
}
}
}
}
}
}
}
}
} Morning Bob,
} Would you mind telling me/us how you accomplish the sandblasting of
} your used stubs. A real materials and methods paragraph would be most
} helpful for us biologists in the audience. I will soon be surrounded by
} bags of the things and I keep on finding more. Have tried sanding and
} some
} other methods, but all either mar the surface unsatisfactorily or leave
} residue.
}
} Thanks in advance,
}
} Fred Monson
}
} Frederick C. Monson, PhD
} Center for Advanced Scientific Imaging(CASI)
} West Chester University of Pennsylvania
} Schmucker Science Center II
} South Church Street
} West Chester, PA, 19383
} eMail: fmonson-at-wcupa.edu
} http://darwin.wcupa.edu/casi/
}
}
} } ----------
} } From: "robert.fowler-at-tdktca.com"-at-sparc5.microscopy.com
} } Sent: Tuesday, December 4, 2001 12:45 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Aluminum Stubs
} }
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} } Given that my curiosity is up per the plating/no plating of the stubs,
} my
} } posting is geared more towards the actual reusing of stubs. For the
past
} } eight years I have been subjecting a set of 50 or so stubs to a sand
} } blasting treatment. You may question the feasibility of a sandblaster
} BUT
} } what is important is the media used to blast with. glass beads in the
} } range
} } of 170-325 mesh are used to effectively remove residue, glue, tape, and
} } any
} } material that resides on the stub. This particular media results in no
} } residue and no tolerance change, although a pure water treatment and
} } ultrasonic wash are utilized. I have had no trouble at all even after
} this
} } amount of time.
} }
} } Robert Fowler
} } Quality Assurance Technician (Failure Analysis)
} } TDK Components USA, Inc.
} } Multilayer Ceramic Capacitor Division
} } 1 TDK Boulevard
} } Peachtree City GA 30269-2051
} } Telephone: (770) 631-0410 Ext.315
} } Fax: (770) 487-1460
} } email: rfowler-at-tdktca.com
} } www.tdk.com
} }
} }
} }
}
}
}
}




From daemon Fri Dec 7 09:02:40 2001



From: Kalvoda Jiri :      dino-at-sci.muni.cz
Date: Fri, 7 Dec 2001 15:21:03 +0100
Subject: apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I apologize to all who recently received a queer email from me. I was hit by
s new virus W32Aliz . Worm which my antivirus failed to detect and which is
spreading in Microsoft Outlook Express without you open any atachment.
Jiri Kalvoda


---
Odchozí zpráva neobsahuje viry.
Zkontrolováno antivirovým systémem AVG (http://www.grisoft.cz).
Verze: 6.0.306 / Virová báze: 166 - datum vydání: 4.12.2001





From daemon Fri Dec 7 09:02:41 2001



From: Kalvoda Jiri :      dino-at-sci.muni.cz
Date: Fri, 7 Dec 2001 15:22:01 +0100
Subject: apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I apologize to all who recently received a queer email from me. I was hit by
s new virus W32Aliz . Worm which my antivirus failed to detect and which is
spreading in Microsoft Outlook Express without you open any atachment.
Jiri Kalvoda


---
Odchozí zpráva neobsahuje viry.
Zkontrolováno antivirovým systémem AVG (http://www.grisoft.cz).
Verze: 6.0.306 / Virová báze: 166 - datum vydání: 4.12.2001






From daemon Fri Dec 7 09:14:28 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 7 Dec 2001 10:08:55 -0500
Subject: EDS depth of penetration

Contents Retrieved from Microscopy Listserver Archives
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These are fundamental questions that you can learn about from a number of text books and courses. I would suggest starting with the SEM and Microanalysis book by Goldstein et al. You could also think about attending the Lehigh Microscopy Short Course which is very good and would teach you these things. David Joy has some Monte Carlo simulation software in the public domain that calculates these things and graphically displays them. You can get them at the EMMPDL - MASLIB - Mac & PC Shareware Libraries at Argonne National Laboratory. The following FTP Site give you access to these Software Libraries. Access is by anonymous Login:

Host : WWW.AMC.ANL.GOV or
the mirror site WWW.MSA.Microscopy.Com

Login:
Username = Anonymous
Password = Your Email Address


You will have to hunt for them. Go to the main listing and look at the titles.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: ATC SEM Laboratory [mailto:atcsem-at-earthlink.net]
Sent: Friday, December 07, 2001 8:09 AM
To: Microscopy-at-sparc5.microscopy.com


Hello,
I have been dealing with SEM/EDS for only 1.5 years and I have a lot to
learn, but recently I have been asked a question: What is the EDS depth of
penetration on my unit? I work with Amray 1645 and it was upgraded by PGT
for imaging and EDS. Could anybody answer the question How deep does the
x-ray beam penetrates through a sample and, if it is varies, how to find out
actual depth?

Thank you in advance.

Pavel
atcsem-at-earthlink.com





From daemon Fri Dec 7 09:14:29 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 7 Dec 2001 09:57:21 -0500
Subject: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you have not lost cooling efficiency in the cold trap at the top of the diffusion pump or in the lines around the diffusion pump, I would wager that it is not diffusion pump oil. Check the cooling lines for adequate flow. If they are plugged, you could get diff pump fluid up in the chamber. Chances are that it is mechanical pump fluid. I trap will help, but you have to maintain it properly. To get rid of the oil in the chamber, there are some things that you could do. I recommend the Evactron system by XEI. I have checked it out (see the M&M 2001 proceedings) and it does clean up hydrocarbon oils in a vacuum chamber. XEI also has an overnight nitrogen purge system that helps clean microscope by using clean dry nitrogen. A colleague at one of our other research sites has this system on an older SEM and it really helps to keep beam contamination down. You could set up your own system with a leak valve and a pump that throttles the vacuum pump. You then keep the system in
the viscous flow regime and it will clean the system. Better yet, you can put heaters on the tubing to heat the nitrogen going into this system and it will improve the "scrubbing" action of the gas molecules on the surface of the chamber.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Friday, December 07, 2001 8:42 AM
To: 'Microscopy-at-MSA.Microscopy.Com'



Dear Listers,

I have an older SEM which over a period of several months seems to
repeatedly develop a small droplet of oil on the EDS detector collimator.

It is a diffusion pumped system. The mechanical backing pump is regularly
serviced and gets oil changes.

I am inclined to think it is time to clean the diffusion pump and change the
oil there. Perhaps over time backstreaming from the mechanical pump has
occurred, causing contamination of the diffusion pump oil with mechanical
pump oil. The latter, which has higher vapor pressure, is evaporating out
of the diffusion pump into the chamber and condensing on the coldest point
there (the detector).

Any opinions on whether this is reasonable or not? Any other possibilities
anyone can think of? I'd hate to change the diffusion pump oil, which is
going to be a big pain, if this is not going to do the trick.

By the way, I'm already planning to install a foreline trap (there currently
isn't one).

Thanks,

Wharton

**********************************************************
Wharton Sinkler
UOP LLC
Des Plaines, IL


From daemon Fri Dec 7 09:40:18 2001



From: Sara Miller :      saram-at-duke.edu
Date: Fri, 7 Dec 2001 10:34:28 -0500 (EST)
Subject: Re: negative staining

Contents Retrieved from Microscopy Listserver Archives
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Hi Richard,

Thanks for the note. I didn't take your remarks as disparaging -
however, I did take advantage of them to have a little fun!

I now work for the NRC Institute for National Measurement Standards,
which is the Canadian equivalent of your NPL, American's NIST, the "world's"
BIPM, etc., so issues of traceability and ISO 17025 take up a lot of my time
now. The fact that there is no officially traceable, high-magnification
TEM calibration sample available has caused me (and probably most
microscopists) some extra headaches. I maintain that TEM calibration
samples based on crystal lattice spacing are fundamental constants of nature
(i.e., "God -traceable") and hence do not require the blessing of a National
Measurement Institute. It does occasionally take a bit of persuasion to
convince the bureaucratically-minded that the whole point of traceability is
not to refer to SI units through a National Measurement Institute, but to
refer to nature through all of the above!

As you implied in your original posting, having a crystal as the basis
of the sample allows self-calibration at better than the 1% level. Backing
that measurement up with XRD is even more convincing. You have a nice
advantage in your calibrated measurements in that you can lattice-match your
alloy layers, and avoid worries about layer thickness variations as a result
of strain. A potential problem with the SiGe marker layers in the MAG*I*CAL
sample was that they were strained layers and hence had a slight variation
in lattice parameter. We worked around that problem by self-calibrating the
thickness of the individual SiGe layer(s)against the pure silicon crystal
substrate. Silicon and TEM are a marriage made in heaven - the low atomic
number of silicon makes the sample still useful for TEMs with accelerating
voltages less than 200 keV. You are also correct about the robustness of Si
relative to InP - in my experience, InP crystals will gratuitously cleave
under any kind of stress, and usually in exactly the region of interest . .
.!

Anyway, I appreciate the opportunity to discuss with you the subtlties
of TEM calibration. The topic doesn't work it's way into social
conversation very often. . .!

Cheers
John


All the best.

Cheers
John


John P. McCaffrey, Ph.D.
Institute for National Measurement Standards
National Research Council of Canada
M-35 Montreal Road Labs
Ottawa, Ontario
K1A 0R6 CANADA

Tel: (613) 993-2715
Fax: (613) 952-9865
Email: john.mccaffrey-at-nrc.ca

----- Original Message -----
} From: "Richard Beanland" {richard.beanland-at-marconi.com}
To: "'John McCaffrey'" {john.mccaffrey-at-nrc.ca}
Cc: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, December 07, 2001 5:13 AM


How are you preparing the Formvar?

Films should be freshly carbon coated. If not fresh, should be less than a
week old--or if you're still having trouble with non-sticking, carbon coat
same day). Also, you can "rejuvenate" them with glow discharge in a vacuum
evaporator for 10-30 sec. You can also irratiate them with UV light for
30-40 min.

If neither is available, try poly-L-lysine (FW: 4000; 0.1% aqueous for a
couple min, then wash in a couple drops of water); drain, dry). Other
possible rx: alcian blue, benzyldimethyl alkylammonium chloride (BAC).


Hayat MA, Miller SE. Negative Staining. McGraw-Hill. 1990.



Sara Miller



On Fri, 7 Dec 2001 JHoffpa464-at-aol.com-at-sparc5.microscopy.com wrote:

} Date: Fri, 07 Dec 2001 08:18:49 EST
} From: JHoffpa464-at-aol.com-at-sparc5.microscopy.com
} To: microscopy-at-sparc5.microscopy.com
} Subject: negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hi all, i am doing collagen extractions from corneas and negative staing them to get the lenghts. my problem is that i am having trouble getting the collagen to stick to the formvar. any suggestions. i am on a deadline to work out the details of this project.
} John
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Fri Dec 7 09:48:42 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Friday, December 07, 2001 6:58 AM
Subject: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
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Try chroma.com They are a great resource and have lots of information on
their web site.

Dave Burton
Optical Specialist
University of Washington
----- Original Message -----
} From: {"ebhan-at-cybermed.ucsd.edu"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 06, 2001 2:32 PM


Dear Wharton and Microscopy List:

The assumption that your oil droplet is roughing pump oil distilled through
the diffusion pump is very reasonable and is the most likely source. Michael
Postek of NIST wrote a paper on this and a method he came up for control of
the problem: "An Approach to the Reduction of Hydrocarbon Contamination in
the Scanning Electron Microscope" Scanning Vol. 18, 269-274 (1996). He used
a controlled leak into the foreline to stop backstreaming and control the
problem. He also dicusses Cryotrps and Nitrogen purge systems as controls. I
have reprints available of this article available for the asking.
Send me your mailing address.
Ric Felton found by IR that his oil drop was coming a oil outgassing
from the Japanese Black Rubber vacuum hose supplied with his SEM.
The oil can also come in from an airlock if the airlock is pumped
continuously by a roughing pump.

I make my living by controling contamination and Oil backsteaming
problems in electron microscopes. Doing business as XEI Scientific, I make
the both a Nitrogen Purge system
and the Evactron SEM-CLEAN Plasma Cleaning system for cleaning Electron
Microscopes. Visit my web site at www. SEMCLEAN.COM for details.

Ronald Vane
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
(650) 369-0133



-----Original Message-----
} From: Sinkler, Wharton {WSinkler-at-uop.com}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}




From daemon Fri Dec 7 10:31:00 2001



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Fri, 7 Dec 2001 10:29:05 -0600
Subject: EM position

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPY TECHNICIAN

The Integrated Microscopy Core, Department of Molecular and Cellular
Biology, Baylor College of Medicine has an immediate full-time opening for
an electron microscopy technician. The Integrated Microscopy Core is a busy,
state-of-the-art facility with a new Hitachi H7500 equipped with Gatan’s new
2kx2k CCD camera, deconvolution, laser scanning confocal, and 2 CCD-based
upright and inverted epifluorescence microscopes. The applicant should have
at least one year of experience in various aspects of sample preparation for
biological TEM including fixation, embedding, ultrathin sectioning, staining
and darkroom procedures. The applicant should have experience in the
operation of TEMs and knowledge of computers. Other duties include
preparation of solutions, embedding media and the maintaining of records.
The position offers opportunities for training in advanced microscopy
techniques, including digital TEM, fluorescence, deconvolution and laser
scanning confocal microscopy. The position requires a minimum of a
Bachelors degree and will start as a Lab Technician II; salary range is in
the 30-40K, commensurate with experience, and the standard Baylor College of
Medicine benefits package.

Send CV and letter of research/technical interests to:

Michael A. Mancini, Ph.D.
Assistant Professor
Director, Integrated Microscopy Core
Department of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX 77030
713 798 8952
713 798 8005 (fax)
mancini-at-bcm.tmc.edu
http://microscopy.bcm.tmc.edu

Baylor College of Medicine is an Equal Opportunity, Affirmative Action and
Equal Access Employer



From daemon Fri Dec 7 11:50:55 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 7 Dec 2001 11:35:43 -0600
Subject: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
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Wharton,

I don't think this is a terribly unusual problem. I have operated two
scopes with EDS detectors that have also developed oil drops on the end of
the snout. The problem is that the collimator is chilled from the liquid
nitrogen in the detector dewar, so any oil vapor in the chamber (and there
is always some) goes right to it and condenses. If your scope is a variable
pressure SEM one thing that helps is to maintain it at 7-10 Pa chamber
pressure when not in use. This keeps a certain amount of gas circulating
through the chamber and helps to flush out the backstreamed vapors. There
is also a product called SEMCLEAN (I think), and I believe it operates on a
similar principle.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Sinkler, Wharton [mailto:WSinkler-at-uop.com]
Sent: Friday, December 07, 2001 7:42 AM
To: 'Microscopy-at-MSA.Microscopy.Com'



Dear Listers,

I have an older SEM which over a period of several months seems to
repeatedly develop a small droplet of oil on the EDS detector collimator.

It is a diffusion pumped system. The mechanical backing pump is regularly
serviced and gets oil changes.

I am inclined to think it is time to clean the diffusion pump and change the
oil there. Perhaps over time backstreaming from the mechanical pump has
occurred, causing contamination of the diffusion pump oil with mechanical
pump oil. The latter, which has higher vapor pressure, is evaporating out
of the diffusion pump into the chamber and condensing on the coldest point
there (the detector).

Any opinions on whether this is reasonable or not? Any other possibilities
anyone can think of? I'd hate to change the diffusion pump oil, which is
going to be a big pain, if this is not going to do the trick.

By the way, I'm already planning to install a foreline trap (there currently
isn't one).

Thanks,

Wharton

**********************************************************
Wharton Sinkler
UOP LLC
Des Plaines, IL


From daemon Fri Dec 7 13:21:01 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 07 Dec 2001 11:09:17 -0800
Subject: Re: SEM spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 09:03 AM 12/7/2001, you wrote:
} Dear Gary,
}
} [snip]



} While I am not familiar with the Amray 1910, I have worked with many other FE
} and conventional SEMs and I think your current measurements are not accurate.
} In general, as beam currents increases, the spot size should also increase,
} which is the opposite of what your data shows.

I would think that this SEM should exhibit the same characteristics as
others would. I agree that the relationship of spot size and specimen
current is whacky. I attribute this to my poor method of measuring
the low specimen currents. As a check, I did the gun brightness test.
It was working as normal. But the current value is probably 100 times
what actual operating specimen current is. Gun brightness is measured
without any final aperture and with condenser lens wide open (-35).


} I also have a graph from JEOL for the spot size vs. beam current for a JEOL
} 840, which apparently is similar to what the other writer has. The graph I
} have has almost a straight line on a log-log grid that goes through 10 nm at
} 10 pA, 50 nm at 1 nA, and 500 nm at 500 nA (this is for a W filament at 30 kV
} and the aperture size is increased as the beam current increases).
} Unfortunately, the JEOL data does not indicate how the "spot diameter" was
} measured.

Assuming we use the same "sharp corner" method, the log-log data means that
the relationship is not linear. My data suggests that it is linear. So again,
my measurements are suspect. The only thing I seem to find believable about
what I measured is that spot size gets smaller at higher CL settings. At least
this is a good correlation direction.


} Spot size vs. beam current data from an older Philips FE SEM at 10 kV with a
} 30 um aperture is as follows: ~0.5 nm at ~10 pA, ~3.5 nm at ~0.55 nA, and
} ~30 nm at ~35 nA.
}
} It is also interesting that in your data, over the full range of CL settings,
} the currents you have measured hardly change, while most SEMs will have a
} full range of several orders of magnitude or more.
}
} If possible, I would suggest you borrow a Keithley 485 picoammeter (or
} similar) to check the current measurements you have made. Note that if the
} sign of the measurement changes at low beam currents, it indicates that there
} is a grounding problem that has a leakage current that is larger (and
} opposite) of what you are measuring. Also note, that on a picoammeter like
} the 485, the displayed reading when connected to a Faraday cup in an SEM will
} always be negative when working correctly.

I ordered a 485. It should be here in two weeks. I'll re-do all measurements
when it gets here. Any special notes I should know about when using it?
It has a BNC input jack so I'll just coax from it to my stage alarm BNC.

What could I do to check the stage grounding situation? The BNC alarm
output is not grounded (shorted). Perhaps I should check the BNC ground
side to be sure it is grounded. That would mess things up for sure. But
the gun brightness measurement would not have worked correctly.


} My background is in using electron microscopes for e-beam lithography. In
} that case, the exposed spot is always larger than the size of the incident
} beam. This results from the fact that as soon as the beam hits, there will
} be scattering and it is the secondary electrons that have the highest
} probability for exposing the resist. For comparison, a high performance FE
} SEM at 30 kV and ~20 pA of current can expose lines down to 10 or 20 nm,
} which puts an upper limit on the beam size for those models at those
} conditions. Since such models have a resolution spec of 1 to 2 nm above 10
} kV, the lithography size is reasonable, since the resolution measurements are
} not limited by a resist resolution or by broadening by secondary electrons.

We use Heidelberg DWL to write masks. The laser makes patterns down to about
0.1u which is all we need at 0.25u feature size. It is probably better
than that
but I have never measured it.


} In general, the approach for measuring resolution is to see how small a gap
} the beam can fit between islands of gold. While this approach does not give
} any information on the profile of the beam as you are trying to measure, it
} certainly gives reasonable data on the "width" of the beam. Quite simply, if
} the image is dark when the beam is positioned in a 5 nm gap between to gold
} islands on carbon, then it is clear that "most" of the beam is hitting the
} carbon and not the gold. It would be interesting if you could correlate your
} width measurements to values from a resolution measurement.

there might be another approach to performing a sanity check. I can
set up some IC process control monitors which have various geometries
of metal fingers meshed between one another. Each set of fingers (E with
lots of fingers sticking out) are meshed together but not touching. With
24VDC or 12VDC in the chamber for the stepper motors and limit switches,
I could connect one E to +12 and bring the other E out through the
picoammeter and place a beam at various points. This ought to cause
current flow when the beam transcends two adjacent fingers. Might be
worth a shot.

I am still searching for a true sharp corner. They are not all that sharp.
At 100KX and above, the detail in their surface shows up and degrades
a sharp delineation at the boundary.

Gary G.



} Joe
} _________________________________________
} Joe Nabity, Ph.D.
} JC Nabity Lithography Systems
} E-Beam Lithography using Commercial SEMs & STEMs
} PO Box 5354, Bozeman, MT 59717 USA
} Voice: (406) 587-0848
} FAX: (406) 586-9514
} E-mail: info-at-jcnabity.com
} Web: www.jcnabity.com



From daemon Fri Dec 7 13:32:58 2001



From: Jill.Webb-at-rssl.com
Date: Fri, 7 Dec 2001 19:25:51 +0000
Subject: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
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Dear Wharton

This is something we had been experiencing with our SEM and our former
beryllium window detector. The X-ray detector had to be removed more than
once to wash oil off the window, in spite of having upgrading the diff pump
oil to Santovac to try to minimise the contamination. I analysed some of
the oil deposited on the collimator by FT-IR and it wasn't Santovac oil, it
was backstreaming diff pump oil. Then when we upgraded the X-ray system a
couple of years ago to a thin (polymer) window detector, I decided
something had to be done about the oil, even though the new detector
surfaces weren't so cool as the previous one's.

One problem with foreline traps is that they slow down pumping speed. So I
cannibalised a much bigger rotary pump from an old mass spec that was being
binned, had it reconditioned, fitted a good foreline trap and haven't
looked back. If you can do the same, I'm sure you will be pleased with the
effort.

Good luck.

Best regards

Jill

Jill Webb
Principal Scientist, Microscopy, RSSL

( Office : +44 (0)118 986 8541 ext 242
( Fax : +44 (0)118 986 8932
* jill.webb-at-rssl.com







"Sinkler, Wharton" {WSinkler-at-uop.com} on 07/12/2001 13:41:57

To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
cc:




Dear Listers,

I have an older SEM which over a period of several months seems to
repeatedly develop a small droplet of oil on the EDS detector collimator.

It is a diffusion pumped system. The mechanical backing pump is regularly
serviced and gets oil changes.

I am inclined to think it is time to clean the diffusion pump and change
the
oil there. Perhaps over time backstreaming from the mechanical pump has
occurred, causing contamination of the diffusion pump oil with mechanical
pump oil. The latter, which has higher vapor pressure, is evaporating out
of the diffusion pump into the chamber and condensing on the coldest point
there (the detector).

Any opinions on whether this is reasonable or not? Any other possibilities
anyone can think of? I'd hate to change the diffusion pump oil, which is
going to be a big pain, if this is not going to do the trick.

By the way, I'm already planning to install a foreline trap (there
currently
isn't one).

Thanks,

Wharton

**********************************************************
Wharton Sinkler
UOP LLC
Des Plaines, IL







********************************************************************
This e-mail is confidential and may contain privileged
information. If you are not the addressee it may be
unlawful for you to read, copy, distribute, disclose or
otherwise use the information in this e-mail. If you are
not the intended recipient please notify us immediately.

Reading Scientific Services Ltd,
The Lord Zuckerman Research Centre,
Whiteknights, PO Box 234, Reading. RG6 6LA.

http://www.rssl.com
http://www.rssl-pharma.com
http://www.productcontamination.com
********************************************************************


From daemon Fri Dec 7 14:51:20 2001



From: Audette, David E. :      David.Audette-at-sylvania.com
Date: Fri, 7 Dec 2001 15:42:00 -0500
Subject: RE: EDS depth of penetration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Pavel,

When I get a question about SEM/EDS analysis depth, I refer people to the
ASTM E 1508-98 "Standard Guide for Quantitative Analysis by Energy
Dispersive Spectroscopy" which is in volume 3.01 if you have the annual
books ASTM publishes. In paragraph 8.2.3, there is a rule of thumb to
estimate the analysis depth where 99% of the x-rays are emitted. The rest
of the guide is also a useful, brief, reference to be familiar with when
doing EDS experiments.

Regards,
Dave Audette
david.audette-at-sylvania.com

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end


From daemon Fri Dec 7 17:41:08 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sat, 8 Dec 2001 10:33:15 +1100
Subject: Re: [Nature Potpourri] Digest Number 147

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

Our website about beetles / coleoptera on www.coleoptera.org has been
updated


You can look on earth from 100's different satellites in real time in
section {other useful things} {Locality search}
You can search fir any locality in section {FAQ}
You can download special program for naturalists and webmasters in section
{software house}
there is Free delta program, true font (male female symbols) and FREE very
good ANTIVIRUS PROGRAM, free font, graphic, bullets in section {Interested
for internet?}
You can search Organism names, Journal Index etc. in section {Other useful
things} {For taxonomists}

We have updated Entomologists glossary on front/index page.

and front index page you can subscribe free newsletter/ discussion forum for
anyone who is interested for beetles / coleoptera.

and more..

Regards

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Fri Dec 7 18:06:36 2001



From: JoAn Hudson :      hudson-at-uoneuro.uoregon.edu
Date: Fri, 7 Dec 2001 17:58:24 -0600
Subject: Book Reviewers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy and Microanalysis Journals is looking for reviewers
for the following books;



Methods in Cellular Imaging

Edited by Ammasi Periasamy



Selected papers on Optical Low-Coherence Reflectometry and Tomography

General Editor, Brian J. Thompson



Selected papers on Optical Microscopy

General Editor, Brian J Thompson



If you would like write a review for the journal please e-mail me and
I will arrange to have the materials sent to you.



Thank you!



JoAn Hudson

Book Review Editor


From daemon Fri Dec 7 23:29:13 2001



From: Jim at Proscitech :      jim-at-proscitech.com
Date: Sat, 8 Dec 2001 15:24:17 +1000
Subject: RE: glass fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soda glass, is used in most and especially the cheaper microscope slides. Soda
glass does fluoresce. If you look at the slide side on and the glass has any
tinge of green, than its soda glass. If you can, just place a couple of
coverslips on the stage - hopefully you would not get fluorescence, proving its
the slide.
The slides to use for any fluorescence applications are the water- white or
superwhite slides. These the manufacturer claims, do not fluoresce at all -
probably true in normal microscopy situations, but one customer with an extra
powerful detection system managed to get some slide fluorescence from the
superwhite slides too.
Disclaimer: ProSciTech supplies Superwhite slides.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, December 08, 2001 12:15 AM, James Pawley
[SMTP:jbpawley-at-facstaff.wisc.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Russell,
}
} If the pattern is the same, the signal is probably still reflected
} light. This signal can be very large compared to fluorescence and it
} is just blasting through (or around) the filters/prism.
}
} Jim Pawley
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Sorry about the subject name, it is a bit misleading. In
} } using a Leica SP2
} } confocal, I have noticed that it is possible to see the cover slip
} } and slide. Why/how is this possible? The setup is essentially this:
} } a coverslip is placed
} } on a slide, which is then placed on the stage (there is nothing between
them,
} }
} } except of course for miscellaneous small dust particles and air). The slide
} } is
} } then scanned with light from a HeNe laser (633nm). When the light detection
} } (wavelengths directed to the PMT's) is set to include 633nm (i.e.
} } 610-650nm), the
} } signal is of course very bright. I am assuming that this is due to
} } reflection. What I am confused about is that if the light detection
} } doesn't include 633nm
} } (sampling from 660-710nm), there is still signal present, and it is
} } in the same
} } pattern as is seen when looking at the reflected light. It is
} } important to note
} } that this pattern is connected to the coverslip and slide somehow,
} } as moving the
} } stage will cause a corresponding change in the image produced by the system.
} } I realize that I may be missing something fairly obvious, but
} } any help would
} } be greatly appreciated.
} }
} } Thanks,
} } Russell
} }
} }
} }
} } --
} } Confocal Imaging Facility Technician
} } Tufts Medical School
} } 136 Harrison Ave.
} } Boston, MA 02111
} } Tel. (617) 636-3795
}
} --
} ****************************************
} Prof. James B. Pawley, Ph. 608-263-3147
} Room 223, Zoology Research Building, FAX 608-265-5315
} 1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
} "A scientist is not one who can answer questions
} but one who can question answers."
} Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Sat Dec 8 23:14:49 2001



From: c. yeh :      buri-at-onebox.com
Date: Sat, 08 Dec 2001 20:58:40 -0800
Subject: pigment reference set & meltmount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I am an art conservator working in Japan and would like to purchase a
pigment reference set and mounting medium but have been unsuccessful
in my web searches.

I seem to recall that McCrone and Fisher sold such pigment reference
sets--with about 40 or so mounted samples of common artist pigments,
but their web pages did not turn up with any such item.

Also, I would like to know what is the best refractive index for medium
used to mount pigments? Is is 1.55? When I was in the U.S. I used Cargille
mounting medium.

Thank you so much for your assistance, since it is quite difficult for
me to find information on this in Japan.


__________________________________________________
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From daemon Sun Dec 9 09:55:36 2001



From: Jim Nicolino :      JNicolino-at-fcol.com
Date: Sun, 09 Dec 2001 10:46:08 -0500
Subject: Re: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Wharton,
It seems like all of the responses to your inquiry are trying to solve
the effect of your problem rather than the cause. The oil that is
condensing is most likely mechanical pump oil if your vacuum system is
working properly. In any vacuum system using a oil charged mechanical
pump and a diffusion pump there will be oil vapors. These oil vapors are
also the cause of contamination spots on the sample after the beam has
stayed in one location for a period of time. Unless you upgrade your
vacuum system to a dry mechanical pump and a turbo molecular pump, oil
vapors will be present.
Now the cause of your problem is the EDX detector itself. EDX detectors
are static vacuum systems. They are initially pumped out; when LN2 is
present in the dewar, which is attached to a cold finger holding all of
the internal components, cryostatic pumping occurs within the EDX
detector. With a well maintained EDX detector, very little thermal loss
is transferred to the external portion of the detector. In your case,
the internal vacuum of your EDX detector is poor and a high thermal loss
causes the EDX tube to be cold and therefore becoming coldfinger within
the vacuum system. This coldfinger ( your EDX tube) is now attracting
the oil vapor present in your SEM and creating the oil droplets.
To solve your problem you need to have a vacuum treatment ( pump-out and
bake-out )performed on your EDX detector.
If you would like additional information concerning EDX detectors,
please visit my web site xraydetectors.com.
Regards,
Jim Nicolino

"Sinkler, Wharton" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} I have an older SEM which over a period of several months seems to
} repeatedly develop a small droplet of oil on the EDS detector collimator.
}
} It is a diffusion pumped system. The mechanical backing pump is regularly
} serviced and gets oil changes.
}
} I am inclined to think it is time to clean the diffusion pump and change the
} oil there. Perhaps over time backstreaming from the mechanical pump has
} occurred, causing contamination of the diffusion pump oil with mechanical
} pump oil. The latter, which has higher vapor pressure, is evaporating out
} of the diffusion pump into the chamber and condensing on the coldest point
} there (the detector).
}
} Any opinions on whether this is reasonable or not? Any other possibilities
} anyone can think of? I'd hate to change the diffusion pump oil, which is
} going to be a big pain, if this is not going to do the trick.
}
} By the way, I'm already planning to install a foreline trap (there currently
} isn't one).
}
} Thanks,
}
} Wharton
}
} **********************************************************
} Wharton Sinkler
} UOP LLC
} Des Plaines, IL


"Sinkler, Wharton" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Listers,
}
} I have an older SEM which over a period of several months seems to
} repeatedly develop a small droplet of oil on the EDS detector collimator.
}
} It is a diffusion pumped system. The mechanical backing pump is regularly
} serviced and gets oil changes.
}
} I am inclined to think it is time to clean the diffusion pump and change the
} oil there. Perhaps over time backstreaming from the mechanical pump has
} occurred, causing contamination of the diffusion pump oil with mechanical
} pump oil. The latter, which has higher vapor pressure, is evaporating out
} of the diffusion pump into the chamber and condensing on the coldest point
} there (the detector).
}
} Any opinions on whether this is reasonable or not? Any other possibilities
} anyone can think of? I'd hate to change the diffusion pump oil, which is
} going to be a big pain, if this is not going to do the trick.
}
} By the way, I'm already planning to install a foreline trap (there currently
} isn't one).
}
} Thanks,
}
} Wharton
}
} **********************************************************
} Wharton Sinkler
} UOP LLC
} Des Plaines, IL


From daemon Sun Dec 9 13:31:26 2001



From: Jill.Webb-at-rssl.com
Date: Sun, 9 Dec 2001 19:20:56 +0000
Subject: Re: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chuck

Apologies. I have just reread my message and can see the mistake. The
analysed oil that had condensed on our detector collimator did not give the
characteristic FT-IR spectrum of a polyphenol ether, e.g. Santovac oil, it
was rotary pump oil (and not diff pump oil). Hence, a foreline trap was
fitted to the rotary pump.

Regards

Jill

Jill Webb
Principal Scientist, Microscopy, RSSL

( Office : +44 (0)118 986 8541 ext 242
( Fax : +44 (0)118 986 8932
* jill.webb-at-rssl.com







"Garber, Charles A." {cgarber-at-2spi.com} on 08/12/2001 05:19:15

To: "jill.webb-at-rssl.com" {jill.webb-at-rssl.com}
cc:



-------- REPLY, End of original message --------








********************************************************************
This e-mail is confidential and may contain privileged
information. If you are not the addressee it may be
unlawful for you to read, copy, distribute, disclose or
otherwise use the information in this e-mail. If you are
not the intended recipient please notify us immediately.

Reading Scientific Services Ltd,
The Lord Zuckerman Research Centre,
Whiteknights, PO Box 234, Reading. RG6 6LA.

http://www.rssl.com
http://www.rssl-pharma.com
http://www.productcontamination.com
********************************************************************


From daemon Sun Dec 9 16:05:10 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 09 Dec 2001 13:56:48 -0800
Subject: Re: Tip flashing: Hitachi S4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My FESEM is Schottky ZrOW, thermally assisted. I believe
that all Hitachi FESEMs are cold cathode. If so, my experience
probably does not apply.

The Schottky filaments have to be "conditioned" prior to use.
This process involves having the gun assembly sitting by itself
and braid strap grounded to Earth ground. The entire gun
electrode set is then brought up to 32KV while a separate
gun chamber ion pump power supply is connected and monitored
for pump current.

The potential is raised slowly, about 500V per 5 minutes or so.
The pump current is constantly watched. If it starts to increase,
the potential is reduced, let sit and then raised again. At some
point, the unit may zap. This drive the pump current way high
and the whole process starts over again. Once the gun assembly
can sit at 32KV for an hour with low pump current and no zapping,
it is then mounted on the column. It should never zap again during
operation at 30KV max rated potential.

When connected to the column and console electronics, a zap
will most likely take out some electronics in the system. This is
why the gun is conditioned off of the column.

I don't have an emission current control. The only computer
controlled setup values are for extractor voltage and suppressor
voltage. Once set, they are not adjusted again under normal
operation.

I would think that any zapping is an indication of dirt or crud on
the gun area. What does Hitachi say about this?

gary g.


At 08:44 AM 12/5/2001, you wrote:

} Dear listers,
}
} This query is directed specifically at users of the Hitachi S4700 FESEM, but
} may also apply more widely to users of FESEM's.
}
} We get a curious result (to me) when flashing the tip. We were advised to
} flash until the emission current reading read about 20uA on the "le" meter.
} The flash intensity adjustment behind the scope was initially set to
} accomplish this in one or two flashes when flashing at an intensity of "2"
} in the Setup-Column menu.
}
} That is what happened for quite some time, however for a while now flashing
} has resulted in values between 7-12 on the "le" meter. The initial flash
} briefly degrades the vacuum at the gun as expected as contamination is
} driven off, but subsequent flashes do not, also as expected. However,
} subsequent flashes usually LOWER the "le" meter value, rather than raise it
} toward our goal of 20. This is the really puzzling part to me. Originally,
} the value increased with each flash, and if an excessive number of flashes
} were required, we would adjust the intensity pot in the rear of the scope.
}
} We normally run the scope at an emission of 10uA, with kV's of 1.0-10.0.
} All vacuum readings are normal, and the scope performs beautifully in terms
} of resolution and brightness.
}
} My questions are:
}
} 1) Why is the "le" value decreasing with multiple flashes?
}
} 2) How dangerous are multiple flashes to the tip?
}
} 3) Does the condition described above indicate any problem with the tip
} and/or scope?
}
} 4) Is flashing actually accomplishing anything if the vacuum gauge shows no
} contamination being driven off?
}
} 5) Is it safe to keep turning up the intensity pot on the rear of the
} scope? (Something tells me no.)
}
} 6) Do I simply not understand what's going on? (Something tells me yes.)
}
} Thanks in advance!
}
} Puzzled in Missouri,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/



From daemon Sun Dec 9 17:26:23 2001



From: Beauregard :      beaurega-at-westol.com
Date: Sun, 09 Dec 2001 18:10:55 -0500
Subject: After market carbon and W basket evaporator units and DP oil

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Hi,

I saved an old Cook CV-301 thermal evaporator from another group from a
dumpster and I rebuilt it. It has two sets of boat holders mounted on the
base plate. The boat gap is 3" and the clamping screws have 3/16" diameter
threads. The bell jar is 12" in diameter by 12" high. Pump down pressure
was improved from 10E-3 to 3 - 5 x10E-6, now.
I have no accessories for the thing. I have no manuals (hint), no
shutter, etc.

I want to set this up as a dedicated old style 1/8" carbon rod and a W
basket (mgs of metal) evaporator. I buy my TEM grids but sometimes they
are too light on carbon or I would like to increase the conductivity of the
carbon 'back coated' formvar grids. Thus, I would like to 'thicken' them
up a bit with carbon. I do NOT want to send big 'boulders' of carbon
smashing through perfect featureless grid films, however.

So I need to buy a carbon evaporation unit of some type.
Should I go with an old style combo unit like Ladd Research sells? Many
think carbon fiber / cord / strings are the way to go and the Ladd unit
does not appear to do that type of C.
Obviously, I would want to have a W basket or W wire setup as a option for
my troubles saving this unit.

Ladd sells both combination and separate units to do both jobs and they can
be shielded. Have you used one of these?
Fullams sells much cheaper priced units, but are unshielded. Have you used
them on a non-EFFA evaporator or port?
Does the carbon rod spring work smoothly and reliably on any of these units?

What other after market units are reliable based on your REAL experiences
and what would you buy today, if you had to do it over again, like me?

One final, low priority, question on DP oil:
The Cook is simpler to use than my Edwards 306A but it only has a 400-450
watt DP heater on a Varian HS2 DP. It calls for 100mls of Dow Corning 704
silicone oil. Not my first choice. I think the heater is too small in
wattage for Santovac based on my experience with a 1964 JEOL JEM 7 TEM oil
conversion attempt. I could try to switch to another DP fluid like Apiezon
A, B or a Fomblin. DC 704 boils at about 215°C. So, have you ever done
this type of oil conversion successfully and what oil did you use?
Alternatively, I also have a degree in Electronic Technology with honors of
the highest distinction and could take the controling circuit board out,
figure out the schematic and convert the design to supply a 1KW or larger
heater. However, I am not sure this HS2 DP can handle a 500 - 600 watt
heater using Santovac for the next 10 years I have to work.
Notes: The HS2 DP seems to be a bit too slow in pumping speed to me and
might be undersized. It is not the original DP unit but looks right
mechanically except for a slight roughing port mis-aligment of 1/4 " at the
4" long vac. hose outlet. The chimney is clean, aligned and assembled
correctly.

Thank you in advance for any PRACTICAL experience(s) you can provide. I am
trying to avoid the trial and error route.

Sincerely,

Paul Beauregard
Senior Research Associate
PPG Industries
Monroeville Technical Center
Monroeville, PA 15146
(724) 325-5131



From daemon Mon Dec 10 00:00:19 2001



From: Gervais Sawyer :      gsawye01-at-bcuc.ac.uk
Date: Mon, 10 Dec 2001 15:11:05 +0000
Subject: LM/SEM Need help on preparing suspended particles

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Gary,

You are correct. The process you describe does not apply to the Hitachi
S-4700 FESEM.

The Hitachi S-4700 is a cold FE. The procedure you describe is for the Amray
"305" gun which is normally brought to 35 KV for one hour or more. If the
engineer tells you 32KV, he is probably cutiing corners.

For the Amray 305 gun:
The zapping you describe is from stray molecules that have a tendency
collect randomly around the gun, including the insulators. I have found that
there are several "thresh-holds": voltages at which the gun arcs. These are
proportional to vacuum integrity. The "thresh-holds" are at 14-15 KV, 24KV
and 30KV. The 305 guns will arc then the vacuum will need to be "cleaned up"
by gettering. We then start the whole conditioning process over again.

Although the indicated vacuum on the Amray CRT reads about 10e-10 Torr, the
actual pressure is closer to 1x10e-8 or 8x10e-9. I have actually measured
this from a calibrated hot cathode gauge.

Amray guns could use with more assistance from an additional ion pump. The
existing ion pump is rated at 2 l/s. This is barely enough to "hold" the
vacuum & seems to be nothing more than an "indicator" gauge. I have
installed a 20 l/s ion pump on the gun and have had considerable success
with less arcing, faster pump down times, better resolution, etc. It is no
mistake that most other Schottky FE guns have this arrangement (LEO,
Hitachi, JEOL).

Now for the Hitachi 4700 Cold FESEM questions:

} 1) Why is the "le" value decreasing with multiple flashes?

It shouldn't. Most Hitachi guns I have seen always increase with multiple
flashes. I would suspect contamination or a gun problem.

} 2) How dangerous are multiple flashes to the tip?

Multiple flashes have a tendency to "blunt" or "round" the tip giving the
tip less filament life but more filament stability. Hitachi actually
recommends multiple flashes to condition a new tip.

} 3) Does the condition described above indicate any problem with the tip
} and/or scope?

See the answer to question #1.

} 4) Is flashing actually accomplishing anything if the vacuum gauge shows
no
} contamination being driven off?

The first flash sould drive off any molecules at the FE tip and, therefore,
give a poor, but temporary, vacuum indication. Successive flashes should
affect the FE tip but will not change the vacuum.

} 5) Is it safe to keep turning up the intensity pot on the rear of the
} scope? (Something tells me no.)

Define "safe" and the amount you are turning up the intensity pot. Hitachi
typically has three flash intensity settings (approprately named: 1,2,3)
with "3" being the most intense. I usually set "1" for 5 ua, "2" for 10 ua
and "3" for 30 ua on the FIRST flash. Sucessive flashes have a tendency to
increase the emission current. This is rather tricky as each filament will
have a different setting. I would guess that anything over 40 & above is
probably melting the FE tip and giving you poorer filament life.

} 6) Do I simply not understand what's going on? (Something tells me yes.)

Based on the depth and type of questions you ask, I think that you do know
what is going on. I also would be concerned also if sucessive flashes
decrease the emission current.

Summary: my guess is that there is something wrong (or going wrong) in the
gun area.

What is the extraction voltages before& after all this?


DISCLAIMER: THE ABOVE ARE BASED UPON MY EXPERIENCE & ARE OF MY OPINION AND
OF MY COMPANY, SCANSERVICE CORP.
TO THOSE THAT WOULD CRITICIZE THE ABOVE BASED UPON A MIS-SPELLED OR
MISPLACED WORD (especially from acadamia) I WOULD ONLY SAY "GET A LIFE".

Any and all CONSTRUCTIVE advice is certainly welcomed.

Regards,

Earl Weltmer



----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Tindall, Randy D." {TindallR-at-missouri.edu}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, December 09, 2001 1:56 PM


I have some samples of tungsten carbide and diamond dust in mineral oil
taken from grinding machines. How do I separate these from the oil for
LM and SEM and disperse them for examination? I need to get these
particles into a solvent so that the particles separate. Oil makes the
particles clump together. Carbides are heavy and sharp and stick to the
sides of all containers so it is just too easy to get a false picture.

Many thanks. Gervais Sawyer. Forest Products Research Centre. UK.



From daemon Mon Dec 10 10:40:49 2001



From: Debby Sherman :      dsherman-at-purdue.edu
Date: 10 Dec 2001 11:19:56 -0500
Subject: plant histo book

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Although there are many excellent books on histochemical techniques for animal tissues, I have been unable to find one still in print that concentrates on plant tissue. I would appreciate any recommendations so I can add this resource to our facility mini-library.

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
West Lafayette, IN 47907



From daemon Mon Dec 10 10:40:49 2001



From: NPGSlithography-at-aol.com
Date: Mon, 10 Dec 2001 11:34:09 EST
Subject: Re: SEM spot size

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Message-ID: {126.89d294b.29463e01-at-aol.com}


Dear Gary,

} I ordered a 485. It should be here in two weeks. I'll re-do all
} measurements
} when it gets here. Any special notes I should know about when using it?
} It has a BNC input jack so I'll just coax from it to my stage alarm BNC.

First, the stage alarm circuit must not be connected while using the
picoammeter. If the BNC is normally connected to the alarm circuit, removing
the cable to the alarm and connecting the picoammeter should be fine. The
stage should have no other grounding path other than through the picoammeter.


(Note that the stage alarm will then be disabled, so it shouldn't be left
this way if inexperienced users will be running the SEM.)

Ideally, the power outlet used by the picoammeter will have the same
grounding as the SEM.

A good reality check is to connect the picoammeter and take a reading while
the SEM is powered up, but the beam is off. With good grounding, a reading
of zero will result. If a non-zero reading is measured, a quick and dirty
solution is to use the "relative" mode of the Keithley 485 in order to take
subsequent readings relative to the beam off baseline. This approach assumes
that any leakage current being measured is a constant. Valid beam current
measurements from a Faraday cup will always be displayed as negative numbers
with the Keithley.

Also, note that a standard BNC cable will generate tens or hundreds of
picoamps through internal friction if it is flexed or even bumped.
Consequently, a relatively short BNC cable that is reasonably motionless will
give the best measurements.

Finally, the Keithley 485 has a local magnetic field that is significant
compared to the typical environmental specs for an SEM. Consequently, for
best imaging, the 485 should be more than ~1 meter from the column. It is
especially important to note that the 485 has a higher local magnetic field
when the front power button is in the off position, than when the meter is
turned on. Consequently, if the meter is close to the column, it must be
unplugged to stop the field.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Mon Dec 10 10:48:12 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 10 Dec 2001 10:43:27 -0600
Subject: Hitachi S400 Tip Flashing

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Thanks to everyone who replied to my questions about flashing the tip in our
FESEM giving us odd flashing current readings. I received some very
informative and helpful replies, as always from this list.

Incidentally, I didn't put the question to the list because of any lack of
helpfulness by Hitachi Instruments. I was just curious about the
experiences of fellow users and what they thought about the theory behind
this. We love this scope!

Happy Holidays,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Mon Dec 10 11:43:18 2001



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Sunday, December 09, 2001 9:00 AM
Subject: Re: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
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Dear Jim and listers,

While Jim Nicolino is says about EDS detectors acting as cold fingers can be
true. However, I wish to point out that not all oil diffusion pumped are
SEMs are the same as far as oiliness. For example, old Hitachi S-570s are
infamous for being oily because they lacked a proper baffle above the
diffusion pump mouth. I have never cleaned a S-570 without finding oil
dripping from the bottom of the baffle plate at the bottom of the chamber.
Hitachi S-2500 and S-2700 which updated the S-570s did not have as severe of
a oil problem because of an improvement to the design.

Also contributing to the effect are choices of pump oil, heater temperature,
cooling water temperature, and crossover pressure. If any of these is wrong
then their will be severe backstreaming from the diffusion pump. Will
Biglow's book "Vacuum Methods in Electron Microscopy" is good reference.

In modern JEOL and Hitachi SEMs that have diffusion pumps rarely does one
find that it is the diffusion pump giving the problem. More common is oil
backstreaming from the load locks. Many a user has replaced the diff pump
with a turbo pump only to get little improvement because of the rotary oil
roughing pumps, poor sample handling, and oily chambers and stages.

Notice: XEI Scientific makes products to remove oil from SEM chambers.
www.SEMCLEAN.com

Ronald Vane
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
(650) 369-0133


-----Original Message-----
} From: Jim Nicolino {JNicolino-at-fcol.com}
To: Sinkler, Wharton {WSinkler-at-uop.com}
Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}



From daemon Mon Dec 10 12:09:48 2001



From: John Twilley :      jtwilley-at-sprynet.com
Date: Mon, 10 Dec 2001 13:11:30 -0500
Subject: Re: pigment reference set & meltmount

Contents Retrieved from Microscopy Listserver Archives
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McCrone continues to sell the small set of reference pigments. Perhaps
you did a search on the "McCrone Associates, Inc." page instead of on
the "McCrone Microscopes and Accessories" pages.

The direct link to the page is:

http://www.mccrone.com/cgi-bin/SoftCart.exe/mac/reference/paintref.html?L+mccrone+svuu9375+1007930922
The choice of mounting medium depends on several factors related to your
own work. It is often convenient to have a mounting medium with higher
refractive index somewhere in the middle of the range of refractive
indices of your samples. First, in many cases a rapid subdivision
between pigment types can be made on the basis of whether they are above
or below the r.i. of the mounting medium.

Second, pigments tend to have high refractive indices for the simple
reason that, in order to function well as pigments, they must be
effective at scattering light. The greater the difference in r.i.
between the organic binder and the pigment, the more effective the
scattering and the greater the opacity of the paint. If you chose a
medium with a low r.i., the large index difference between the particles
and the medium will make it more difficult to observe the optical
properties and shape of very fine particles.

In general, Far Eastern art done in aqueous media makes greater use of
low r.i. pigments in the form of clays, micaceous minerals and so on.
So the nature of the art which you spend time on is something to
consider as well.

The softening temperature of the mounting medium is a factor to
consider. It is convenient to have a material which remains viscous
near room temperature so that particles may be observed while rolling
under the cover slip. On the other hand, if you store mounted samples
for long periods, such media will cold-flow and require you to store
your slide cases standing on end so that the slides remain horizontal to
keep the coverslips from sliding off.

Finally, I would caution against placing too great a confidence in
pigment identifications based primarily on comparison to a limited
reference set. Many conservation studies suffer from erroneous pigment
identifications based PLM comparison to the most similar example in a
limited reference set rather than an actual identification. There is no
substitute for instrumental analysis to supplement microscopy.

Disclaimer: I am not associated with McCrone A. & C. and have no
interest the company.

John Twilley
Art Conservation Scientist

c. yeh wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} I am an art conservator working in Japan and would like to purchase a
} pigment reference set and mounting medium but have been unsuccessful
} in my web searches.
}
} I seem to recall that McCrone and Fisher sold such pigment reference
} sets--with about 40 or so mounted samples of common artist pigments,
} but their web pages did not turn up with any such item.
}
} Also, I would like to know what is the best refractive index for medium
} used to mount pigments? Is is 1.55? When I was in the U.S. I used Cargille
} mounting medium.
}
} Thank you so much for your assistance, since it is quite difficult for
} me to find information on this in Japan.
}
}
} __________________________________________________
} FREE voicemail, email, and fax...all in one place.
} Sign Up Now! http://www.onebox.com
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}
}
}



From daemon Mon Dec 10 12:32:18 2001



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 10 Dec 2001 12:23:39 -0600
Subject: AMRAY 1610T manuals?

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I recently acquired an AMRAY 1610T with PGT 4000 EDS.
(Thanks, Melissa!)

I hope to restore it to working condition in my
spare time. The manuals that were included appear to
be incomplete copies. For example, the SEM manual
doesn't include schematics, but the vacuum logic
manual does.

Are there also service manuals for this? What might
be my best route to getting a set of complete manuals?

- John



From daemon Mon Dec 10 12:43:37 2001



From: Frank Thomas :      thomasf-at-gsca.NRCan.gc.ca
Date: Mon, 10 Dec 2001 14:16:14 -0400
Subject: Joysticks

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Here's a question - can joysticks be serviced? The one that controls the
stage movement on our ElectroScan E3 has been getting a little wonky lately;
movement is quite jerky, especially in side-to-side motion. I'm thinking
maybe there's a build-up of crud or something in there that might be causing
this.
There are four small Phillips-head screws that seem to hold the joystick in
place on the control panel, and I wonder if we could get at the mechanism
that way.
Has anyone out there ever worked on their own joystick? (There's just no way
to say that without it sounding kind of funny...)


F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada



From daemon Mon Dec 10 14:14:48 2001



From: Shanling Shi :      Shanling.Shi-at-unilever.com
Date: Mon, 10 Dec 2001 15:06:31 -0500
Subject: Oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

We have a small room for cryoesctioning and storage of a liquid nitrogen
container. For the safety concern, I would like to get a oxygen sensor or alarm
to monitor the oxygen level. Does anyone konw where I can get this kind of
device? Any suggestion will be appreciated. Thanks in advance.

Shanling

Shanling Shi
Advanced Imaging & Measurement
Unilever Research US
45 River Road
Edgewater, NJ 07020
201-840-2340
Shanling.Shi-at-unilever.com



From daemon Mon Dec 10 16:17:35 2001



From: Jim Romanow :      bsgphy3-at-uconnvm.uconn.edu
Date: Mon, 10 Dec 2001 17:03:33 -0500
Subject: Oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
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Hello Shanling,

We are using a:
PriorityOne
Model 6810-0056-M
AirAware O2 Monitor
telephone 877 746-1266
www.prio1.com

Distributed by:
Lab Safety Supply
Box 1368
Janesville, WI 53547-1368
800 356-0783
800 543-9910 fax

Lab Safety's part# is OF-67816
Cost was: $610 (6 months ago)

This AirAware model is AC powered (Comes with an AC adaptor, no batteries
to worry about.), simple to install, and has a loud alarm. The sensor is
relatively inexpensive and easy to replace. We use it in our freeze
fracture/cryo microtome room.

Good Luck,
Jim

Disclaimer:
We have no monitary interest in PriorityOne or Lab Safety Supply.







}
} Hello everyone,
}
} We have a small room for cryoesctioning and storage of a liquid nitrogen
} container. For the safety concern, I would like to get a oxygen sensor or
} alarm
} to monitor the oxygen level. Does anyone konw where I can get this kind of
} device? Any suggestion will be appreciated. Thanks in advance.
}
} Shanling
}
} Shanling Shi
} Advanced Imaging & Measurement
} Unilever Research US
} 45 River Road
} Edgewater, NJ 07020
} 201-840-2340
} Shanling.Shi-at-unilever.com
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
Unit 2242
Storrs, CT 06269-2242
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Mon Dec 10 16:38:13 2001



From: Juan Hernandez :      jherna2-at-po-box.mcgill.ca
Date: Mon, 10 Dec 2001 19:31:17 -0500
Subject: Upgrading a EDX tracor Northern TN5500

Contents Retrieved from Microscopy Listserver Archives
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Dear, All:

I was wondering if it is possible to upgrade an old Tracor Northern
TN5500 EDX into
a PC based system.

Thanks for your time.

Juan





From daemon Mon Dec 10 17:19:14 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 10 Dec 2001 15:08:26 -0800
Subject: Re: AMRAY 1610T manuals?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does it have an ion pump on the gun chamber? If so,
it is set up to handle LaB6, otherwise W only.

What seems to be the problem with the system at present?
Is it connected and powered? Check all vacuum connections.
A good option is to remove the standpipe (if ion pump is present)
and replace with Lesker butterfly valve and serrated stainless steel
flex pipe with KF fittings. The standard vertical stand pipe can
be a a leaker.

The UVACOS vacuum board is pretty much standard for
all 1000 series SEMs. The 1610T added the Balzers 240
turbo and controller. The slow scan nature of this system
makes it difficult to use. I brought a 1610T to life about four
years ago. I moved on to an 1830 and and now have a
1910FESEM. There is much similarity between these
extremes of models.

If there are specific sections you need, I have a complete
manual for AMR1000.

gary


At 10:23 AM 12/10/2001, you wrote:

} I recently acquired an AMRAY 1610T with PGT 4000 EDS.
} (Thanks, Melissa!)
}
} I hope to restore it to working condition in my
} spare time. The manuals that were included appear to
} be incomplete copies. For example, the SEM manual
} doesn't include schematics, but the vacuum logic
} manual does.
}
} Are there also service manuals for this? What might
} be my best route to getting a set of complete manuals?
}
} - John



From daemon Mon Dec 10 17:40:27 2001



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Tue, 11 Dec 2001 10:34:13 +1100
Subject: Seventh Annual UBC Live-Cell course

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Announcing the Seventh Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells, June 10 - 20, 2002

Sixth, Post-course Workshop on, 3D Image Processing, June 22 - 24, 2002

Organized by Prof. James Pawley, University of Wisconsin-Madison

in association with the, UBC Brain Research Centre, Prof. Max
Cynader, Director.
University of British Columbia, Vancouver, BC, Canada

(CORRECTION!! some early brochures were sent out with an incorrect
URL. Course info can be found at: ht tp://www.3dcourse.ubc.ca )

THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary
improvement in our ability to view living cells. To help convert
this promise to reality for a wider selection of biological
scientists, the organizers have designed an intensive eleven-day
residential course concentrating on all aspects of the 3D Microscopy
of Living Cells. Sponsored by the Brain Research Centre at the
University of British Columbia, it will be held in June of 2002. The
course includes 4 days on 2D techniques, 5 days of 3D techniques and
2 days on 3D measurement and display. It includes everything from
basic microscopy to confocal and multiphoton microscopy. A half-day
Pre-course is offered for those wishing to brush up on (very!) basic
optics.

Topics include:
o Quantitative 2D light microscopy
o 3D Imaging in confocal and widefield
o Fluorescent and backscattered light signals
o Digitization: The Nyquist Criterion
o Poisson noise QE and S/N.
o Lasers and laser tweezers
o Objectives and aberration correction
o Scanning-systems: AODs, mirrors, disks
o Wide field/deconvolution techniques
o Detectors: operation and performance
o Optimal pinhole size/photon efficiency
o Dye design, characteristics and use
o How to keep your cells alive
o Multi-photon excitation
o Measuring ion concentrations
o Display and measurement of 3D data

Morning lecture/demonstrations lead to hands-on laboratory exercises
each afternoon utilizing most of the commercial instruments currently
available for 3D microscopic imaging. Students will work in groups
of 3 or 4 throughout the discussion and laboratory sessions, and may
complete a live-cell 3D study on their own specimens. In the first 6
years, over 160 students from 25 countries have attended. Last year,
12 separate 3D microscopical workstations were each scheduled for
over 30 hours of student use under the supervision of an
international faculty of 17 who represent the state-of-the-art in
live-cell microscopy. Including manufacturers representatives, the
teacher/student ratio will be almost 2:1.

INTERNATIONAL FACULTY
o Stephen Adams University of California-SD
o Dan Axelrod University of Michigan
o Mark Cannell University of Auckland
o Milton Charlton University of Toronto
o Ping Chin Cheng SUNY, Buffalo
o Stefan Hell Max Planck Institute, Goettingen
o Alan Hibbs BioCon, Melbourne, Australia
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andres Kriete Tissue Informatics, Pittsburgh
o Glen MacDonald Virginia Bloedel Hearing Inst, WA
o Irina Majoul Max Planck Institute, Goettingen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Technology
o Chip Shook Eppendorf Scientific,Albequerque, NM
o Jim Turner Wadsworth Institute, NY
o Michael Weis Agriculture Canada

TUITION
Course tuition is $2,250 US and includes lunches and generous snacks.
On receipt of 50% deposit, all students will receive preliminary
group assignments and a copy of the textbook, Handbook of Biological
Confocal Microscopy, (Plenum, 1995). The tuition fee includes the
Opening Reception, the Manufacturer's Reception, and the Beach Party,
and a handout binder. Accommodations and other meals are not
included. The Pre-course is $100 US

APPLICATIONS
Applicants will submit an application to assess knowledge level and
field of interest. Enrollment will be limited to 24 - 32
participants (depending on equipment availability). Selection will
be made on the basis of background and perceived need. Those with
little previous LM experience will be provided with basic texts to
read before the course begins, and should take the Pre-course.

Application forms and other course information from this and past
years can be downloaded from the WWW site at

h ttp://www.3dcourse.ubc.ca/home.html

or obtained from:

Prof. James Pawley,
Zoology Department.,
1117 W. Johnson Drive,
Madison, WI.
Phone: 608-263-3147 fax. 608-265-5315
Email: jbpawley-at-facstaff.wisc.edu

IMPORTANT DATES
Applications must be received by Mar. 15, 2002
Deposit due Apr. 15, 2002
Registration 5:00 - 7:00 pm Sunday, June 9, 2002
Intro. Lecture 7:00 PM, Sunday, June 9, 2002
Last class ends with lunch, Thursday, June 20, 2002

--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From daemon Mon Dec 10 19:57:24 2001



From: IAN HALLETT :      ihallett-at-hortresearch.co.nz
Date: Tue, 11 Dec 2001 14:50:06 +1200
Subject: Autoradiography emulsions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a query from a collegue carrying out autoradiography of an
uneven surfaced structure. She has found that standard
autoradiographic plates are not suitable and is interested in using a
liquid emulsion or stripping film. I have directed her towards Ilford
as a source of a liquid emulsion - has anyone any other suggestion
and is stripping film (Kodak AR 10) still available.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________
The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From daemon Tue Dec 11 06:21:20 2001



From: Micha Bayer :      M.Bayer-at-rbge.org.uk
Date: Tue, 11 Dec 2001 12:00:20 BST
Subject: LM: deconvolution/diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

we are using a light microscope to acquire brightfield images of
diatoms, which we then want to process by computer. However, the
presence of diffraction effects around the edges of the images is
causing us problems.

Does anyone know of any software, preferably free, which can
deconvolve an image to reduce the effects of diffraction?

thank you

Micha Bayer
______________________________

Dr. Micha Bayer
Royal Botanic Garden Edinburgh
20A Inverleith Row
Edinburgh EH3 5LR
Scotland, U.K.
Tel. (+44) (0)131-248 2915
Fax (+44) (0)131-248 2901
Project Homepage at http://www.rbge.org.uk/research/Diadist/index.htm
RBGE home page at http://www.rbge.org.uk
______________________________


From daemon Tue Dec 11 08:07:21 2001



From: David Wilbur :      dwilbu01-at-emerald.tufts.edu
Date: Tue, 11 Dec 2001 08:58:37 -0500
Subject: Re: Oxygen monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oxygen monitors are pretty standard in hospital MRI centers. I have some
experience with ones from Enmet. URL is http://www.enmet.com/medical.html. Sensor
cells need to be replaced at least once a year, and the calibration drifts as the
cells age. Although calibration kits are available, I alway just calibrated to the
known O2 level in the air, at a place and time when no cryogens were present.

Dave

Shanling Shi wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} Hello everyone,
}
} We have a small room for cryoesctioning and storage of a liquid nitrogen
} container. For the safety concern, I would like to get a oxygen sensor or alarm
} to monitor the oxygen level. Does anyone konw where I can get this kind of
} device? Any suggestion will be appreciated. Thanks in advance.
}
} Shanling
}
} Shanling Shi
} Advanced Imaging & Measurement
} Unilever Research US
} 45 River Road
} Edgewater, NJ 07020
} 201-840-2340
} Shanling.Shi-at-unilever.com

--
__________________________________
David J. Wilbur, Ph.D.
Instrumentation Specialist
Department of Chemistry
Tufts University
voice: 617-627-2163
Fax: 617-627-3443
email: dwilbu01-at-tufts.edu
__________________________________




From daemon Tue Dec 11 08:15:13 2001



From: Jessica Wagner :      WagnerJS-at-missouri.edu
Date: Tue, 11 Dec 2001 08:08:14 -0600
Subject: Retiga 1300 camera from QImaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

Does anyone have any information or opinion, positive or negative,
about Q-Imaging and their Retiga 1300 cooled ccd camera? Their web
site is www.qimaging.com. We would be using the camera for
fluorescence imaging (with deconvolution) and bright field, dark
field, DIC, phase imaging. Please feel free to contact me on or off
the list.

Thanks for any info,
--
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
Jessica Wagner
Molecular Cytology Core Facility
University of Missouri
573-882-4895


From daemon Tue Dec 11 08:33:48 2001



From: Karli Fitzelle :      fitzelle.1-at-osu.edu
Date: Tue, 11 Dec 2001 09:28:37 -0500
Subject: EM -Plant Pathology Book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
Does anybody out there know of a decent EM book that focuses on pathology
in agricultural systems (i.e. chickens, maize and insects [as vectors])?



From daemon Tue Dec 11 09:58:58 2001



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 11 Dec 2001 15:46:44 -0000
Subject: Cracking Hairs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Cleaning out my "delete" file I have just noticed a request for a method to
enable the cracking of human hair. I have been involved with this type of
"problem" many times so set out below is a method which we have found works
well for LM as well as SEM. We have even used the technique to investigate
failures in freezer bags!

1. Drill two or three holes through two SEM stubs placed face to face.
2. Pass the fibres through the holes.
3. Fix them in place with a water based carbon solution, make sure the
fibre/stub interface is well wetted.
4. When fully dry place in liquid nitrogen CARE!!.
5. When the bubbling stops lift out CARE!!
6. Place on an insulating surface and with a blade strike the interface
between the two stubs - the system will fracture.
7. When dried off you have two sets of surfaces to look at in LM or SEM.

It works great on a number of differing media other than fibres, the only
snag is they must not be affected by the water base.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com



From daemon Tue Dec 11 12:36:53 2001



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Tue, 11 Dec 2001 13:33:33 -0500
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
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I am posting the following for a colleague. Please respond directly to him.

Marie

**********************

Electron Microscopy Technician/Research Assistant



This is a full time position of which 70% of time will be devoted to
electron microscopy research support and 30% to a combination of research
support in light microscopy immunocytochemistry, cell biology and general
laboratory maintenance. Percentages might be adjusted to research demands.
Responsibilities include: Transmission electron microscopy (TEM)
preparation, immunolabeling and TEM observation and documentation of
perfused brain tissues; preparation and immunolabeling of perfused brain
tissue samples for light microscopy immunocytochemistry and
immunofluorescence; cell culture; preparation of buffer solutions;
laboratory maintenance; supervision of laboratory ordering, receiving and
record keeping, among others.
Qualifications: Background in biological sciences. Candidates with
experience in biological transmission electron microscopy and good
communication skills are preferred. Salary commensurate with experience.

Submit curriculum vitae plus names, addresses and phone numbers of three
references to:

Prof. Angel L. de Blas
Dept. Physiology & Neurobiology
3107 Horsebarn Hill Rd; U-4156
Storrs, CT 06269-4156
Ph: (860) 486-3285
Fax: (860) 486-3303
e-mail: deblas-at-oracle.pnb.uconn.edu


We encourage applications from under-represented groups including
minorities, women and people with disabilities.




Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2242
University of Connecticut
Storrs, CT 06269-2242
Phone: 860-486-3588
Fax: 860-486-6369




From daemon Tue Dec 11 17:42:37 2001



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Wed, 12 Dec 2001 10:45:13 +1100
Subject: EM Position in Sydney Australia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following position for a microscopist is being advertised in a major
Australian University.

The University of New South Wales is 30 minutes to the central business
district of Sydney and 10 minutes to Coogee Beach. The University of NSW
is widely regarded as the premier Australian research University and
encourages a vigorous research program. The University was the cradle for
two major advances in electron microscopy:

1. The Robinson scintillator backscatter detector

2. The Environmental scanning electron microscope



The monetary reward may not seem generous compared with US or European
salaries.

But this is compensated for by the strong local purchasing power of the
Australian dollar and the benefit that a good lifestyle is much less
expensive and more accessible in Sydney than in other international
capitals.


Sydney is noted for its mild climate; beaches; opera house; cosmopolitan
atmosphere; busy arts scene; access to aquatic sports like swimming,
surfing, sailing, SCUBA diving; international standard sports facilities;
quality of cuisine and value of wines; excellent educational facilities;
high quality of medical care etc. Sydney is an ideal city in which to
raise children.


You can learn about NSW from the State Government website

http://www.nsw.gov.au/


You can learn about Sydney from the tourism website

http://www.visitnsw.com.au/0300/0300.asp


You can learn about the University at http://www.unsw.edu.au and about
the Electron Microscope Unit at http://srv.emunit.unsw.edu.au


You can direct questions to

p.munroe-at-unsw.edu.au


{bold} {color} {param} ffff,0000,0000 {/param} {bigger} Deputy Director

UNIVERSITY OF NEW SOUTH WALES

ELECTRON MICROSCOPE UNIT

REF. 1293EMAIL

{/bigger} {/color} {/bold}

The Electron Microscope Unit is a central infrastructural research
facility, containing eleven principal instruments with a staff of ten.
The Unit supports a very wide range of research projects from across the
University and its success is based on a strong client-focused
orientation. The Unit seeks a dynamic electron microscopist, ideally with
a strong background in biomedical or polymer research. The successful
applicant will be expected to assist the Unit's Director in the
day-to-day management of the Unit, provide expertise in microscopy to a
wide range of research projects in biomedical and organic materials and
provide appropriate training in microscopy to the Unit's users. The
Deputy Director will also develop and carry out research projects, not
only related to their own interests, but also in collaboration with other
academic staff within the University.


The successful applicant will also be expected to take up a fractional
(20%) appointment as Lecturer or Senior Lecturer in a School relevant to
their background and experience and will contribute to the teaching
effort of that School and to supervise research students as
appropriate.


{bold} Additional essential criteria {/bold} for appointment at Senior
Lecturer level: a significant record of publication, a demonstrated
track record in attracting research funding and prior experience in
managing an electron microscope facility.


{bold} Essential criteria: {/bold} a PhD; proven capacity to undertake
high quality research; track record of publications in internationally
refereed journals; proven experience in teaching; excellent
interpersonal, oral and written communication skills; very high level
practical experience and theoretical ability in electron microscopy;
ability to implement OHS principles and equity and diversity policies and
programs.

Desirable criteria: track record in attracting research funding;
experience in management of a research laboratory.


{bold} The salary range {/bold} will be commensurate with Lecturer A$52,173
- A$61,957 per year or Senior Lecturer is A$63,912 - A$73,695 per year
depending on qualifications and experience.

Membership of a University approved superannuation scheme is a condition
of employment.

Enquiries may be directed to Electron Microscope Unit Director, Associate
Professor Paul Munroe, on telephone (61 2) 9385 4435, facsimile (61 2)
9385 6400 or email p.munroe-at-unsw.edu.au.

Applications close 7 February 2002.


APPLICATION PROCEDURE

Applicants should submit written applications systematically addressing
the selection

criteria, QUOTING REFERENCE NUMBER. Include business and private
telephone numbers; a complete resume, (copies of academic transcript and
qualifications where appropriate); and the names, addresses (and
preferably email addresses or facsimile numbers) of at least two referees
to: The Recruitment Officer, Human Resources, UNSW Sydney 2052, email:
recruitment-at-unsw.edu.au or facsimile (61 2) 9662 2832 by applications
close date.

www.unsw.edu.au




Dr. Mel Dickson,

Deputy Director, The Electron Microscope Unit,

Adjunct Associate Professor, School of Microbiology & Immunology

The University of New South Wales

UNSW SYDNEY 2052

Australia.

Phone +612 9385 6383 Fax +612 9385 6400

http://srv.emunit.unsw.edu.au/


From daemon Tue Dec 11 17:44:11 2001



From: tartenon-at-netscape.net
Date: Tue, 11 Dec 2001 18:39:13 -0500
Subject: RE: Olympus IMT-2 filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Chroma Inc at www.chroma.com (the website is only a guess) but they manufacture custom made filters

Regards

Alfredo
"ebhan-at-cybermed.ucsd.edu"-at-sparc5.microscopy.com wrote:

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From daemon Tue Dec 11 17:50:32 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Tue, 11 Dec 2001 15:46:11 -0800
Subject: Re: LM: deconvolution/diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How are they mounted? I have no experience with diatoms, but for what it's worth, what about minimizing the refraction BEFORE the light gets to the computer? I mean if the diatoms are in water you may be able to increase the refractive index of the medium you are using (by adding dextran, polyvinyl alcohol, etc) to nearly match that of the specimen, so that the object's refraction is less of a problem.

OptiPrep (TM) (aka iodixanol) is miscible with water and has a very high refractive index, 1.40 for a 40% solution, which is near the R.I. of silica. Another contrast medium, Omnipaque (TM) (iohexol) is lower in R.I. but may be less expensive.

I would guess that this approach would even improve deconvolved images of diatoms in water. Does anyone have experience with this?

Richard

Richard Thrift, PhD

(858) 625-2424 Richard_Thrift-at-SkyePharma.com
SkyePharma, Inc
10450 Science Center Drive
San Diego, CA, 92121 USA

} } } "Micha Bayer" {M.Bayer-at-rbge.org.uk} 12/11/01 12:00:20 PM } } }
Hi,

we are using a light microscope to acquire brightfield images of
diatoms, which we then want to process by computer. However, the
presence of diffraction effects around the edges of the images is
causing us problems.

Does anyone know of any software, preferably free, which can
deconvolve an image to reduce the effects of diffraction?

thank you

Micha Bayer
______________________________

Dr. Micha Bayer
Royal Botanic Garden Edinburgh
20A Inverleith Row
Edinburgh EH3 5LR
Scotland, U.K.
Tel. (+44) (0)131-248 2915
Fax (+44) (0)131-248 2901
Project Homepage at http://www.rbge.org.uk/research/Diadist/index.htm
RBGE home page at http://www.rbge.org.uk
______________________________




From daemon Tue Dec 11 22:12:31 2001



From: Smartech :      smartech-at-optonline.net
Date: Tue, 11 Dec 2001 23:13:53 -0500
Subject: digital microscopy and stereo microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With the advent of digital cameras that can be hooked into either eye piece
of a stereo microscope, the collection of stereo pairs seems very simple.
And with inkjet printers and programs like Adobe Photoshop we now have a
great deal of freedom. Has anyone found any simple solutions for printing
and presenting the stereo pairs?

Ric

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Dec 12 01:19:29 2001



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Wed, 12 Dec 2001 08:20:38 +0100
Subject: Re: LM: deconvolution/diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I have never worked with diatoms and have never experienced the problem you
describe. I have however worked with image processing and analysis. One
thing that experience has taught me is that it is best to present as 'good'
or 'pure' an image as possible to the computer rather than use the software
to 'clean it up'. Such processing will change your image/data in some way
which may be undesirable (depends what you are doing).

Could you suspend them in something else?
Use a different optical setup?

I would be interested to know if you find an answer.

Good luck.


Nadolig Llawen a Blwddyn Newydd Dda / God Jul och Gott Nytt År / Merry
Christmas and a Happy New Year

Med vänliga hälsningar/With best wishes

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-impi.ki.se

Tel +46 8 728 3734 Fax +46 8 728 3688

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi,
Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Dept of Biomedical Laboratory Science,
Lindhagensgatan 92, Box 12773,
S 112 96, Stockholm
Sweden

OBS! Besöksadress: Lindhagensgatan 92
NB! Visiting address:Lindhagensgatan 92


From daemon Wed Dec 12 05:50:08 2001



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 12 Dec 2001 09:06:34 -0600
Subject: Re: LM: deconvolution/diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Fred Monson -

Fred -

I tried to reply directly to you but it bounced for some reason, so this
time I'm sending to the whole list, assuming you'll see it this way. (If
anyone else is having problems with a wonky joystick, read on to learn
what's fixed mine.)

Frank Thomas
GSC Atlantic



----- Original Message -----
} From: "Frank Thomas" {thomasf-at-agc.bio.ns.ca}
To: "Monson, Frederick C." {fmonson-at-wcupa.edu}
Sent: Tuesday, December 11, 2001 3:07 PM


Hello Micha,
I would agree that index matching your mounting media to the refractive
index of silica should alleviate the diffraction problems you
describe. One important concern is the method by which you are introducing
contrast into the specimen for visualization. When the refractive indices
are matched, the diatoms will be invisible under standard brightfield
illumination. Phase contrast and Nomarski DIC depend on the changes in
refractive index at boundries between areas of differing RI to enhance
contrast.
I have no experience with imaging diatoms, but I seem to recall
that darkfield illumination is a popular way to image diatoms, although
this method is also dependant on at least a slight mismatch in the
refractive index of the specimen and mountant. You might be able to get
away with a very close match, however.
The numerical aperture of the lens you are using to image the
diatoms should be as large as possible. I'm not sure how big your diatoms
are, but using a higher numerical aperture objective (preferably oil
immersion) may help. If your diatoms are small enough to approach the
diffraction-limited resolution limit of the objective lens you are using
switch to a higher power or better quality objective (if you have that option).
Good luck!
-Karl G.



At 12:00 PM 12/11/2001 +0100, Micha Bayer wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________
Karl Garsha
Specialist in Light Microscopy
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu



From daemon Wed Dec 12 09:16:22 2001



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 12 Dec 2001 08:03:12 -0700
Subject: digital microscopy and stereo microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ric,

I just wanted to point you to our analySIS software and the Stereo module.
You can take a stereo pair, and display that anaglyphically (red and green),
and with the correct glasses you see a 3D image (you can of course also
print that). Alternatively, you can have the software calculate the 3D
surface of the Object from the stereo pair and then display your object in
3D on the computer. The software was initially written for stereo imaging on
an SEM, but there is no reason it should not work on images from a stereo
microscope.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Smartech [mailto:smartech-at-optonline.net]
Sent: Tuesday, December 11, 2001 9:14 PM
To: To all on the list


With the advent of digital cameras that can be hooked into either eye piece
of a stereo microscope, the collection of stereo pairs seems very simple.
And with inkjet printers and programs like Adobe Photoshop we now have a
great deal of freedom. Has anyone found any simple solutions for printing
and presenting the stereo pairs?

Ric

SMARTech
860-485-5054
www.semguy.com
19 Cornwall Drive
Goshen CT 06756



From daemon Wed Dec 12 10:07:53 2001



From: DrJohnRuss-at-aol.com
Date: Wed, 12 Dec 2001 11:00:01 EST
Subject: Re: digital microscopy and stereo microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 12/12/01 10:24:00 AM, mb-at-Soft-Imaging.com writes:

} I just wanted to point you to our analySIS software and the Stereo module.
} You can take a stereo pair, and display that anaglyphically (red and green),
} and with the correct glasses you see a 3D image (you can of course also
} print that). Alternatively, you can have the software calculate the 3D
} surface of the Object from the stereo pair and then display your object
} in 3D on the computer. The software was initially written for stereo imaging
} on an SEM, but there is no reason it should not work on images from a stereo
} microscope.

Since you originally asked about Photoshop, I'd like to point out that
combining two images as an anaglyph is straightforward using the Photoshop
channels command (put the left image into the red channel, the right one into
the blue and green). And for measuring the parallax and generating a height
map or reconstructed 3D image of the surface you can use the Image Processing
Tool Kit plug-ins for Photoshop. One of the examples on the web site (see
http://www.ReindeerGraphics.com/foveapro2/surface.html) in fact uses images
of a small fossil captured through a stereo microscope using an inexpensive
Nikon digital camera.


From daemon Wed Dec 12 10:21:47 2001



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 12 Dec 2001 11:26:00 -0500
Subject: RE: Oil backstreaming

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The backstreaming of oil from diffusion pump systems into the
specimen chambers of electron microscopes has been a problem that has
been around for a long time, and one that is very hard to eliminate.
This was a matter that I addressed as fully as I could while writing
my book on 'Vacuum Methods in Electron Microscopy' (see
http://www.2spi.com/catalog/books/book48.html and
http://www.pup.princeton.edu/titles/6484.html for a description).
Dealing with backstreaming from rotary vane pumps is described on
pages 144 to 150, and from oil diffusion pumps on pages 190 to 200.
Perhaps some of the information presented there would be of help.
--
Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
3062 Dow Bldg.; 2300 Hayward St.
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237


From daemon Wed Dec 12 12:23:44 2001



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 12 Dec 2001 10:13:55 -0800 (PST)
Subject: demonstration for K-6th grade students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I was going to volunteer to give a 60 minute science demonstration to
students at local grade schools and was looking for ideas for projects. I
came up with some things such as having the students look at organisms in
pond water in light microscopes, maybe some SEM photos of common insects
(which may not be hands on enough to interest the kids), or teaching them
how to pan for gold. The idea is to capture the interest of students and
encourage them to study science.

Could you forward any ideas you may have?
Thanks,
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Wed Dec 12 12:43:13 2001



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Wed, 12 Dec 2001 10:35:12 -0800
Subject: Coverslip culture chambers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Çan anyone direct me to a manufacturer of 2-chamber coverslips for cell
culture that use #1.5 thickness coverslips? So far, I'm only finding #1 thickness.

Thanks,
Glen

--
Glen MacDonald
Microscopy and Imaging Facility
University of Washington Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156 (206) 616-1828 fax
**************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
**************************************************************************


From daemon Wed Dec 12 12:57:18 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 12 Dec 2001 12:55:10 -0600
Subject: Nikon Diaphot 100X oil lens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We're looking for a 100X planapo oil lens (NA = 1.4) for a Nikon
Diaphot TMD, tube length 160 mm.
If anyone has one in excellent condition to sell, please let me know.
Vendor replies welcome.
Phil
--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Dec 12 13:32:14 2001



From: Richard Thrift :      Richard_Thrift-at-SkyePharma.com
Date: Wed, 12 Dec 2001 11:24:35 -0800
Subject: Re: LM: deconvolution/diffraction, matching RI's of sample &

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I guarantee that they won't match! Without a bit of directed effort, at least, and if they do happen to match, it is easily fixed by adjusting the concentration of polymer.

With just a tiny bit of mismatch between sample & medium, the sample can be imaged easily by tweaking the contrast on the camera, even if it is not so easily seen by eye.

Another trick which works for latex beads & may work for silica is to add a bit of Nile Red to the suspension. The dye will stick to the surface & fluoresce, while it is almost nonfluorescent in water.

Richard

} } } James Pawley {jbpawley-at-facstaff.wisc.edu} 12/12/01 10:08:29 AM } } }
Problem is that when the RI finally does match, bright field contrast
disappears.

Jim P.
} ------------------------------------------------------------------------
} How are they mounted? I have no experience with diatoms, but for
} what it's worth, what about minimizing the refraction BEFORE the
} light gets to the computer? I mean if the diatoms are in water you
} may be able to increase the refractive index of the medium you are
} using (by adding dextran, polyvinyl alcohol, etc) to nearly match
} that of the specimen, so that the object's refraction is less of a
} problem.
}
} OptiPrep (TM) (aka iodixanol) is miscible with water and has a very
} high refractive index, 1.40 for a 40% solution, which is near the
} R.I. of silica. Another contrast medium, Omnipaque (TM) (iohexol) is
} lower in R.I. but may be less expensive.
}
} I would guess that this approach would even improve deconvolved
} images of diatoms in water. Does anyone have experience with this?
}
} Richard
}
} Richard Thrift, PhD
}
} (858) 625-2424 Richard_Thrift-at-SkyePharma.com
} SkyePharma, Inc
} 10450 Science Center Drive
} San Diego, CA, 92121 USA
}
} } } } "Micha Bayer" {M.Bayer-at-rbge.org.uk} 12/11/01 12:00:20 PM } } }
} Hi,
}
} we are using a light microscope to acquire brightfield images of
} diatoms, which we then want to process by computer. However, the
} presence of diffraction effects around the edges of the images is
} causing us problems.
}
} Does anyone know of any software, preferably free, which can
} deconvolve an image to reduce the effects of diffraction?
}
} thank you
}
} Micha Bayer
} ______________________________
}
} Dr. Micha Bayer
} Royal Botanic Garden Edinburgh
} 20A Inverleith Row
} Edinburgh EH3 5LR
} Scotland, U.K.
} Tel. (+44) (0)131-248 2915
} Fax (+44) (0)131-248 2901
} Project Homepage at http://www.rbge.org.uk/research/Diadist/index.htm
} RBGE home page at http://www.rbge.org.uk
} ______________________________

--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39



From daemon Wed Dec 12 16:01:33 2001



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Wed, 12 Dec 2001 13:52:35 -0800 (PST)
Subject: Re: demonstration for K-6th grade students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon - Try liquid nitrogen experiments. These are always a hit with
kids although a but risky so caution is advised. You could freeze and
break rubber bands, shatter a rose, inflate a balloon with LN gas boil off
(use a plastic soda bottle) then deflate it by placing back into LN.
There are lots of other ideas as well but this can get you started. Good
luck.

Dave

On Wed, 12 Dec 2001, Gordon Vrololjak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} I was going to volunteer to give a 60 minute science demonstration to
} students at local grade schools and was looking for ideas for projects. I
} came up with some things such as having the students look at organisms in
} pond water in light microscopes, maybe some SEM photos of common insects
} (which may not be hands on enough to interest the kids), or teaching them
} how to pan for gold. The idea is to capture the interest of students and
} encourage them to study science.
}
} Could you forward any ideas you may have?
} Thanks,
} Gordon
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
}
}



From daemon Wed Dec 12 16:29:26 2001



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 12 Dec 2001 14:20:52 -0800
Subject: TEM coccolithophorids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Season's Greetings:

I am looking for anyone with experience looking at coccolithophorids using TEM.

We need to look at sections. The guys are only about 5 um in diameter. I
have done some thin layer embedding and individual cell selection before,
but never on anything this small. I will be checking other sources of
information about techniques, but thought I would ask here too,

Any hints or tips on fixing, embedding, etc. that might help?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Dec 13 06:13:54 2001



From: mega_star7tgt7-at-excite.com
Date: Wed, 12 Dec 2001 23:50:16 -0500
Subject: .....improve reception 23 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Poor reception and dropped calls getting you down?
Get the cell phone booster antenna.
Easy to install works on any cell phone


http://61.16.69.50/booster



Removes to: CellSales4-at-excite.com With "PsRemove" in the subject














Poor reception and dropped calls getting you down?
Get the cell phone booster antenna.
Easy to install works on any cell phone













From daemon Thu Dec 13 07:16:12 2001



From: Gerhard Frank :      Gerhard.Frank-at-ww.uni-erlangen.de
Date: Thu, 13 Dec 2001 14:08:36 +0100
Subject: Re: TEM coccolithophorids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List, dear Jon,
In our lab people from the institute of crystallograhpy investigated coccoliths
using TEM.
They just used "powders" in plane view.
If there is any method to prepare these algae so that the algae or the
coccoliths can be sectioned and investigated individually I would be very glad
to receive this inforamtion, too.
Thank you very much.
Gerhard Frank

Jon Krupp schrieb:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Season's Greetings:
}
} I am looking for anyone with experience looking at coccolithophorids using TEM.
}
} We need to look at sections. The guys are only about 5 um in diameter. I
} have done some thin layer embedding and individual cell selection before,
} but never on anything this small. I will be checking other sources of
} information about techniques, but thought I would ask here too,
}
} Any hints or tips on fixing, embedding, etc. that might help?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

--
Gerhard Frank
Central Facility for High Resolution Electron Microscopy
UNIVERSITAET ERLANGEN-NUERNBERG
Institut fuer Werkstoffwissenschaften VII
Cauerstr. 6, D-91058 Erlangen, Germany Gerhard.Frank-at-ww.uni.erlangen.de


From daemon Thu Dec 13 07:42:36 2001



From: joe.p.neilly-at-abbott.com
Date: Thu, 13 Dec 2001 07:36:13 -0600
Subject: Re: demonstration for K-6th grade students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

Caroline Schooley has assembled several microscopy based school activities on
the Project Micro web page (
http://www.msa.microscopy.com/ProjectMicro/ClassroomActivities.html). The one
I am familiar with, The Beanie Baby Mystery, is easy to do with a few
stereomicroscopes or magnifying glasses. It can take 30-45 minutes depending
on the size of the class and the story can be changed for the age of the
students.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202




Gordon Vrololjak
{gvrdolja-at-nature.Ber To: microscopy-at-sparc5.microscopy.com
keley.EDU} cc:
Subject: demonstration for K-6th grade students
12/12/01 12:13 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,
I was going to volunteer to give a 60 minute science demonstration to
students at local grade schools and was looking for ideas for projects. I
came up with some things such as having the students look at organisms in
pond water in light microscopes, maybe some SEM photos of common insects
(which may not be hands on enough to interest the kids), or teaching them
how to pan for gold. The idea is to capture the interest of students and
encourage them to study science.

Could you forward any ideas you may have?
Thanks,
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope
Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793








From daemon Thu Dec 13 08:37:54 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 13 Dec 2001 08:34:47 -0600
Subject: Re: Coverslip culture chambers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nalge Nunc, their Lab Tek II chambered coverglass. They also make 1,
4, and 8 well coverslips with 1.5 thickness coverglasses.
I don't have a phone number or URL, as our dealings are with the
local distributor.
Phil

} Çan anyone direct me to a manufacturer of 2-chamber coverslips for cell
} culture that use #1.5 thickness coverslips? So far, I'm only
} finding #1 thickness.
}
} Thanks,
} Glen
}
} --
} Glen MacDonald
} Microscopy and Imaging Facility
} University of Washington Core for Communication Research
} Virginia Merrill Bloedel Hearing Research Center
} Box 357923
} University of Washington
} Seattle, WA 98195-7923
} glenmac-at-u.washington.edu
} (206) 616-4156 (206) 616-1828 fax
} **************************************************************************
} C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
} **************************************************************************

--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Thu Dec 13 09:24:59 2001



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 13 Dec 2001 15:22:18 +0000 ()
Subject: SEM specimens: removing gold with KI/I2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon -

General preparation methods for light microscopy and SEM examination of
coccolithophorids are nicely summarized on pages 330-331 of "Plankton
Stratigraphy" Bolli, Saunders and Perch-Neilsen (Editors), 1985, Cambridge
University Press. They don't, however, say anything about sectioning them.
For biostratigraphy, where it's important to know exactly what species
you're dealing with, you normally want the little guys as whole as possible,
and id's are made on the basis of (mostly) morphology. I bet your local
Earth Sciences library has this book, anyway.
Are you at liberty to say why you're interested in sectioning them?

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia

----- Original Message -----
} From: "Jon Krupp" {jmkrupp-at-cats.ucsc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, December 12, 2001 6:20 PM



Calling All Microscopists,

We have often removed sputtered gold from SEM specimens with a solution of
potassium iodide / iodine complex. However, I've forgotten the
quantitative formulation. Does anyone know how much of KI and of I2 to
use?

+-----------------------------------------+
Robert H.Olley
J.J.Thomson Physical Laboratory
University of Reading
Whiteknights
Reading RG6 6AF
England
+-----------------------------------------+
Phone:
{direct line +44 (0) 118 9318572
{University internal extension 7867
Fax: +44 (0) 118 9750203
Email: R.H.Olley-at-reading.ac.uk
URL: http://www.reading.ac.uk/~spsolley
+-----------------------------------------+




From daemon Thu Dec 13 09:29:37 2001



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Thu, 13 Dec 2001 10:31:32 -0400
Subject: Re: demonstration for K-6th grade students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon,

I would like to direct you to a wonderful program called "Project
MICRO" (details can be found on the MSA website):
http://www.msa.microscopy.com/Project Micro

Basically it is a program to introduce microscopic explorations to
elementary and junior high school students. I am a member and
corresponding secretary of MSA's local affiliate, New England Society
for Microscopy, and our group has wholeheartedly embraced Project
MICRO. We actually raised money and put together three kits
(inlcudes all the materials, dissecting and simple monocular
microscopes). Several members have used the program in their local
schools. We even had a workshop at our annual Woods Hole Meeting
(Woods Hole, MA) a few years back to encourage members to get
involved. I am hoping to run a Saturday Workshop at a local school
sometime in January.

The program utilizes a workbook "Microscopic Explorations"-a GEMS
Festival Teacher's Guide, which was put together at the Lawrence Hall
of Science at the University of California at Berkeley.

It sounds like a good program for you. You could get a kit and run a
workshop with teachers in a local school. Students and teachers are
overwhelmingly supportive.


Take a look......

Peggy Sherwood






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu


From daemon Thu Dec 13 10:35:05 2001



From: Tom Moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Thu, 13 Dec 2001 10:24:47 -0600
Subject: Re: Coverslip culture chambers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check out:

http://nunc.nalgenunc.com/products/catalog/cellculture/LabTekIIChamberSlideSystem.html

Tom

At 08:34 AM 12/13/2001 -0600, you wrote:
} Microscopy-at-sparc5.microscopy.com

Thomas Moninger (thomas-moninger-at-uiowa.edu)
University of Iowa Central Microscopy Research Facility (www.uiowa.edu/~cemrf)
View expressed are my own.



From daemon Thu Dec 13 12:33:20 2001



From: Oakley, Jeff :      oakleyj-at-rayovac.com
Date: Thu, 13 Dec 2001 12:24:37 -0600
Subject: EDS analysis / "Chemical shift"

Contents Retrieved from Microscopy Listserver Archives
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I am currently working on an EDS analysis project on carbon / fluorine
matrices. Usually carbon starts as the taller peak, and remains so
throughout the acquisition. Occasionally, however, when starting an
spectrum acquisition I will see the fluorine peak start as the taller peak,
then slow to become the shorter peak.

I was told by an observer that there is a phenomenon known as "chemical
shift," that could have an impact on the amount of certain elements in the
interaction volume of a sample. This person didn't know much about it other
than it could give you inaccurate quant results.

Is there such a thing as chemical shift, and if so, could someone explain
what exactly is happening during the process?

Thanks,


Jeff Oakley
Rayovac Corporation


From daemon Thu Dec 13 14:21:14 2001



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 13 Dec 2001 14:11:20 -0600
Subject: Re: EDS analysis / "Chemical shift"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This sounds like a problem with terminology. I would not call it chemical
shift but probably would call it devolatilization.

When someone says "chemical shift", I usually think of a shift in the
energy or wavelength of the x-ray lines due to the chemical environment of
an atom. For example, sulfur as sulfide, as sulfate, and as thiol each have
slightly different peak energies. That is something that can be observed
and can be a problem with wavelength dispersive analysis. However, the
resolution is not so good to be able to observe such a shift, unless you
have one of the new microcalorimeter detectors.

I might also consider a shift of intensity between peaks as a chemical
shift. I remember seeing a paper about the L-lines of nickel. Depending on
the amount of alloying element (Al, I think), the relative intensity of the
L-lines changed dramatically. The explanation was that the electronic
environment changed so that different transitions giving rise to L-lines
were more favorable as there was more or less of the alloying element. This
is quite visible by EDS, but not that common.

I sincerely doubt that you are seeing either of these effects.

What you are probably seeing is a change in chemical composition with
continued exposure to the electron beam. That does lead to a shift in the
chemical composition, but I reserve the phrase chemical shift for what I
explained above. You are either preferentially volatilizing one component
compared to another, or you are changing the components through heating.
This is not uncommon, particularly at higher currents and voltages.

You might try a number of things to reduce the energy dumped into your
sample. You might try dropping your beam current. If you are doing SEM, you
might also reduce the voltage. You might try spreading a beam over an area
(or larger area) rather than using a point analysis so that the energy is
spread out. (This presumes your sample is homogeneous.)

You might also try a much shorter exposure, or successive exposures to try
to capture the initial composition. I know our Tracor Northern TN-2000 had
a feature for painting a window for an element and plotting the intensity
of that window over time - you picked the time interval per channel.
However, I don't think that feature has been on any of our last three EDS
systems. It would be really helpful for what you are facing. There are
probably ways to fake your way through with what you do have. \

I hope this helps.
Warren

At 12:24 PM 12/13/01 -0600, you wrote:

} I am currently working on an EDS analysis project on carbon / fluorine
} matrices. Usually carbon starts as the taller peak, and remains so
} throughout the acquisition. Occasionally, however, when starting an
} spectrum acquisition I will see the fluorine peak start as the taller peak,
} then slow to become the shorter peak.
}
} I was told by an observer that there is a phenomenon known as "chemical
} shift," that could have an impact on the amount of certain elements in the
} interaction volume of a sample. This person didn't know much about it other
} than it could give you inaccurate quant results.
}
} Is there such a thing as chemical shift, and if so, could someone explain
} what exactly is happening during the process?
}
} Thanks,
}
}
} Jeff Oakley
} Rayovac Corporation

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Thu Dec 13 16:02:50 2001



From: Jesse Rodrigues :      Jesse_Rodrigues-at-kopin.com
Date: Thu, 13 Dec 2001 16:59:12 -0500
Subject: Staining of GaN materials

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Hi,

I am looking for help finding staining chemistries or procedures,
which may be used to enhance imaging of GaN based material layers. Any
information you could pass along would be helpful.

Thank you,
Jesse Rodrigues

Device Processing Manager
Kopin Corporation
695 Myles Standish Blvd.
Taunton, MA 02780
Ph#(508) 824-6696
Fax#(508) 824-6958
email:jrodrigues-at-kopin.com




From daemon Thu Dec 13 17:13:48 2001



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 13 Dec 2001 18:06:46 -0500
Subject: Removing gold layers

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert H.Olley wrote:
=============================================================

We have often removed sputtered gold from SEM specimens with a solution of
potassium iodide / iodine complex. However, I've forgotten the quantitative
formulation. Does anyone know how much of KI and of I2 to use?
===============================================================
I know it goes against conventional wisdom, but in the past we have been
able to remove the sputtered gold layer using an oxygen plasma in one of our
Plasma Prep II plasma etchers. I have never had anyone explain to me why it
would do this but it apparently does. A typical gold thickness would come
off in about thirty or so minutes. We made this discovery when trying to
come up with a good demonstration of the effect of etching as a function of
time on some ordinary clay coated paper. To etch a little more we had to
first etch off the applied gold layer for the previous step. This was done
some years ago before the days or the more modern techniques that often
times require no coating at all. We have the results from that study on a
sign we use in our exhibit stands but don't have it yet on our website. If
you would like to see the results, ask me when ever you see SPI at a trade
show exhibition. I usually have it with me on display.

For some samples, the liquid method would change the sample and that would
certainly be the case for paper.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask
that any reply to this message be by way of the "reply" feature on your
software, so that the entire string of correspondence can come back to us
and all be in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From daemon Thu Dec 13 18:47:35 2001



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Thu, 13 Dec 2001 16:39:44 -0800
Subject: RE: EDS analysis / "Chemical shift"

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It's also possible that carbon is increasing during the analysis due to
hydrocarbon contamination of the sample or microscope, a common situation in
e-beam analysis. You might try monitoring the count rates for the C and F
x-rays to see whether the carbon is increasing or fluorine is being lost.

Larry Thomas
Pacific Northwest National Laboratory
P.O. Box 999
Mail Stop P8-16
Richland, WA, USA

email: Larry.Thomas-at-pnl.gov
phone: (509) 372-0793
fax; (509) 376-6308



----------
From: Warren E Straszheim
Sent: Thursday, December 13, 2001 12:11 PM
To: Oakley, Jeff
Cc: Microscopy-at-sparc5.microscopy.com
Subject: Re: EDS analysis / "Chemical shift"


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-----------------------------------------------------------------------.


This sounds like a problem with terminology. I would not call it
chemical
shift but probably would call it devolatilization.

When someone says "chemical shift", I usually think of a shift in
the
energy or wavelength of the x-ray lines due to the chemical
environment of
an atom. For example, sulfur as sulfide, as sulfate, and as thiol
each have
slightly different peak energies. That is something that can be
observed
and can be a problem with wavelength dispersive analysis. However,
the
resolution is not so good to be able to observe such a shift, unless
you
have one of the new microcalorimeter detectors.

I might also consider a shift of intensity between peaks as a
chemical
shift. I remember seeing a paper about the L-lines of nickel.
Depending on
the amount of alloying element (Al, I think), the relative intensity
of the
L-lines changed dramatically. The explanation was that the
electronic
environment changed so that different transitions giving rise to
L-lines
were more favorable as there was more or less of the alloying
element. This
is quite visible by EDS, but not that common.

I sincerely doubt that you are seeing either of these effects.

What you are probably seeing is a change in chemical composition
with
continued exposure to the electron beam. That does lead to a shift
in the
chemical composition, but I reserve the phrase chemical shift for
what I
explained above. You are either preferentially volatilizing one
component
compared to another, or you are changing the components through
heating.
This is not uncommon, particularly at higher currents and voltages.

You might try a number of things to reduce the energy dumped into
your
sample. You might try dropping your beam current. If you are doing
SEM, you
might also reduce the voltage. You might try spreading a beam over
an area
(or larger area) rather than using a point analysis so that the
energy is
spread out. (This presumes your sample is homogeneous.)

You might also try a much shorter exposure, or successive exposures
to try
to capture the initial composition. I know our Tracor Northern
TN-2000 had
a feature for painting a window for an element and plotting the
intensity
of that window over time - you picked the time interval per channel.

However, I don't think that feature has been on any of our last
three EDS
systems. It would be really helpful for what you are facing. There
are
probably ways to fake your way through with what you do have. \

I hope this helps.
Warren

At 12:24 PM 12/13/01 -0600, you wrote:

} I am currently working on an EDS analysis project on carbon /
fluorine
} matrices. Usually carbon starts as the taller peak, and remains so
} throughout the acquisition. Occasionally, however, when starting
an
} spectrum acquisition I will see the fluorine peak start as the
taller peak,
} then slow to become the shorter peak.
}
} I was told by an observer that there is a phenomenon known as
"chemical
} shift," that could have an impact on the amount of certain elements
in the
} interaction volume of a sample. This person didn't know much about
it other
} than it could give you inaccurate quant results.
}
} Is there such a thing as chemical shift, and if so, could someone
explain
} what exactly is happening during the process?
}
} Thanks,
}
}
} Jeff Oakley
} Rayovac Corporation

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking





From daemon Thu Dec 13 19:52:56 2001



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu (by way of Nestor J.
Date: Thu, 13 Dec 2001 19:46:36 -0600
Subject: anti-fade agent

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Dear Listmembers,

Does anyone know of an antifade agent that works for liver-cell microscopy
systems? We're having trouble with fading of the fluorescent signal.
Thanks in advance.

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Thu Dec 13 23:19:10 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 13 Dec 2001 21:10:22 -0800
Subject: IC cross sections -- with high Z contrast

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hello listers:

I am working ICs which have an upper and lower
TiW barrier metal layer between AlSi. The problem is
that the huge extreme of Z makes SE and BSE imaging
big time difficult. I tried moderating the range using
Au/Pd (60/40%) but it makes little difference. This
coating was about 40A thick.

Does anyone have a suggestion about how to normalize
the Z range of materials in this type of specimen so that
either SE or BSE images would show reasonable contrast?
If anyone would like to see my pathetic images at this time,
I can put them on one of my web sites.

Here are the killer (Z values):
Al=13
Si=14
Ti=22
W=74 (low % but huge impact on image contrast)

I have thought about Silver (Z47) and Zirconium (Z40)
to try to even out the overall Z range of the specimen.
Unfortunately, Anatech does not make Zirconium
targets for the Hummer VII. I have available Au, Pd, Pt, silver, or
air.

Am I at the limits of this coater and its target materials?

TIA,
gary g.



From daemon Fri Dec 14 02:35:38 2001



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Fri, 14 Dec 2001 14:00:21 +0530
Subject: Gatan Duo Mill 600 TMP - servicing information requested

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Dear list members:

Our ion mill has developed a vacuum problem. After checking and servicing various components including replacing the whisperlock with the blanking tool, we have narrowed down the possible defect to be with the vac valve (airlock evacuation valve) on the right mill. The vacuum deteriorates significantly when the right mill vac button is depressed and remains at the poor vac continuously. This is irrespective of whether the whisperlock or the blanking tool is installed . Among the spares, we have spare o-rings for the vac and vent valves, but we do not have any information regarding how these are to be greased / replaced or for the valve to be serviced. No apparent screws / nuts / washers etc are found. Any information regarding the servicing of these parts will be greatly appreciated since we do not have a service contract on the instrument. Our instrument is nearly 10 years old and we have been maintaining it ourselves for most part of the period.

With Thanks and Best wishes to all list members,
----
Divakar R
Physical Metallurgy Section,
Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----





From daemon Fri Dec 14 03:10:55 2001



From: Allen Sampson :      ars-at-sem.com
Date: Fri, 14 Dec 2001 03:04:53 -0800
Subject: EDS analysis / "Chemical shift"

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I wish you had said more about the materials you are looking at. The
electron beam causes considerable heating in a sample, and if there is free
fluorine in the sample, not chemically bonded, then the action of this
heating and the vacuum could cause migration of the fluorine. As mentioned
by another poster, 'chemical shift' in x-ray spectroscopy refers to the
actual shifts in peak locations caused by chemical bonds altering the
quantum energy levels of the electron shells. This effect will only be
seen on fully focusing Johannson wavelength spectrometers or the more
recent microcalorimeter systems, developed at NIST.

One other possibility is an instrumental problem. Problems in the DAC
circuitry of an EDS can result in odd effects, particularily at the low
end. Normally, something like this would be heat related and probably show
up as the instrument is first warming up. Is it possible that the high
fluorine peaks that slowly decrease are found primarily on the first spe
ctrum or two in a run of samples?


Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


-----Original Message-----
} From: Oakley, Jeff [SMTP:oakleyj-at-rayovac.com]
Sent: Thursday, December 13, 2001 10:25 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


I am currently working on an EDS analysis project on carbon / fluorine
matrices. Usually carbon starts as the taller peak, and remains so
throughout the acquisition. Occasionally, however, when starting an
spectrum acquisition I will see the fluorine peak start as the taller peak,
then slow to become the shorter peak.

I was told by an observer that there is a phenomenon known as "chemical
shift," that could have an impact on the amount of certain elements in the
interaction volume of a sample. This person didn't know much about it
other
than it could give you inaccurate quant results.

Is there such a thing as chemical shift, and if so, could someone explain
what exactly is happening during the process?

Thanks,


Jeff Oakley
Rayovac Corporation



From daemon Fri Dec 14 05:53:56 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 14 Dec 2001 06:50:17 -0500
Subject: Re: IC cross sections -- with high Z contrast

Contents Retrieved from Microscopy Listserver Archives
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Gary,
You should have a gamma function that will allow non-linear
amplification on your AMRAY. Have you tried that?

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hello listers:
}
} I am working ICs which have an upper and lower
} TiW barrier metal layer between AlSi. The problem is
} that the huge extreme of Z makes SE and BSE imaging
} big time difficult. I tried moderating the range using
} Au/Pd (60/40%) but it makes little difference. This
} coating was about 40A thick.
}
} Does anyone have a suggestion about how to normalize
} the Z range of materials in this type of specimen so that
} either SE or BSE images would show reasonable contrast?
} If anyone would like to see my pathetic images at this time,
} I can put them on one of my web sites.
}
} Here are the killer (Z values):
} Al=13
} Si=14
} Ti=22
} W=74 (low % but huge impact on image contrast)
}
} I have thought about Silver (Z47) and Zirconium (Z40)
} to try to even out the overall Z range of the specimen.
} Unfortunately, Anatech does not make Zirconium
} targets for the Hummer VII. I have available Au, Pd, Pt, silver, or
} air.
}
} Am I at the limits of this coater and its target materials?
}
} TIA,
} gary g.
}
}
}
}



From daemon Fri Dec 14 07:41:13 2001



From: DCiaburri-at-gdds.com
Date: Fri, 14 Dec 2001 08:16:14 -0500
Subject: Re: IC cross sections -- with high Z contrast

Contents Retrieved from Microscopy Listserver Archives
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Gary,

Sounds like gamma filtering would help you. If you have that option on
your SEM, give it a try.

Diane Ciaburi






Gary Gaugler {gary-at-gaugler.com}
12/14/01 12:10 AM


To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
cc:
Subject: IC cross sections -- with high Z contrast


hello listers:

I am working ICs which have an upper and lower
TiW barrier metal layer between AlSi. The problem is
that the huge extreme of Z makes SE and BSE imaging
big time difficult. I tried moderating the range using
Au/Pd (60/40%) but it makes little difference. This
coating was about 40A thick.

Does anyone have a suggestion about how to normalize
the Z range of materials in this type of specimen so that
either SE or BSE images would show reasonable contrast?
If anyone would like to see my pathetic images at this time,
I can put them on one of my web sites.

Here are the killer (Z values):
Al=13
Si=14
Ti=22
W=74 (low % but huge impact on image contrast)

I have thought about Silver (Z47) and Zirconium (Z40)
to try to even out the overall Z range of the specimen.
Unfortunately, Anatech does not make Zirconium
targets for the Hummer VII. I have available Au, Pd, Pt, silver, or
air.

Am I at the limits of this coater and its target materials?

TIA,
gary g.







From daemon Fri Dec 14 08:02:12 2001



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 14 Dec 2001 07:56:50 -0600
Subject: RE: IC cross sections -- with high Z contrast

Contents Retrieved from Microscopy Listserver Archives
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Hello Gary,

Unfortunately, application of a coating will not solve your problem. The
only effect will be to reduce sensitivity to changes in Z under the coating.
The same effect could be had by just reducing the gain/contrast control.
The Z ratio remains the same under the coating. If the contrast/gain is
increased to compensate for the sensitivity loss generated by an applied
coating, you will be back where you started, but with a degraded signal to
noise ratio. I have graphics of this effect in my homegrown "SEM Primer"
booklet.

The problem is common for me. Sometimes I want to see very small Z changes
when the overall range is huge. You ought to try heterogeneous
mixes/compounds like C, O, Al, and U!!!

To my knowledge, there is no really *good* solution to maintaining high BSE
sensitivity while not saturating, but I have used two methods.

1) Multiple images: 2, maybe 3 images, each covering a band of atomic
numbers without black/white saturation. The images may be used separately,
or colored and an overlay composite made using an image editor.

2) Use non-linear video processing. I modified my (previous GW Elec.) BSE
system by adding a clipping diode with a variable reference bias . Could
set white clipping just about anywhere in the output range. The
clipping/limiting was not "hard" so that some signal variation would make it
through. This had the effect of reducing effective gain/brightness on the
high end. The degree of gain compression was a function of the original
gain & brightness and the new adjustment.

Although only viewable effectively using a software package, another
approach would to use more than 256 shades/8 bit gray scale
(hardware/software permitting). For Example, a 32 bit gray scale image
would likely work fine, but you could only "see" a portion of the range at
any time.

Woody White
McDermott Technology, Inc.



} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Friday, December 14, 2001 12:10 AM
} To: MSA listserver
} Subject: IC cross sections -- with high Z contrast
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} hello listers:
}
} I am working ICs which have an upper and lower
} TiW barrier metal layer between AlSi. The problem is
} that the huge extreme of Z makes SE and BSE imaging
} big time difficult. I tried moderating the range using
} Au/Pd (60/40%) but it makes little difference. This
} coating was about 40A thick.
}
} Does anyone have a suggestion about how to normalize
} the Z range of materials in this type of specimen so that
} either SE or BSE images would show reasonable contrast?
} If anyone would like to see my pathetic images at this time,
} I can put them on one of my web sites.
}
} Here are the killer (Z values):
} Al=13
} Si=14
} Ti=22
} W=74 (low % but huge impact on image contrast)
}
} I have thought about Silver (Z47) and Zirconium (Z40)
} to try to even out the overall Z range of the specimen.
} Unfortunately, Anatech does not make Zirconium
} targets for the Hummer VII. I have available Au, Pd, Pt, silver, or
} air.
}
} Am I at the limits of this coater and its target materials?
}
} TIA,
} gary g.
}
}


From daemon Fri Dec 14 08:26:06 2001



From: Anthony J. Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 14 Dec 2001 09:18:40 -0500
Subject: Re: EDS analysis / "Chemical shift"

Contents Retrieved from Microscopy Listserver Archives
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I second all the comments made by Warren Straszheim in reply to this
posting, but would also suggest what seems to me to be an equally likely
possibility given the elements in question - contamination.

Jeff does not mention the form of the samples or the conditions of the
experiment, but the observation that the carbon signal increases with
analysis time is very common, and is the result of the build-up of
contamination resulting from the break-down of hydrocarbon molecules
diffusing around the sample surface (sometimes originating from the
microscope vacuum, sometime3s carried in with the sample, often
both). This is observed much more at high magnifications or in point mode,
and both in the SEM and the STEM/TEM. Frequently, a careful examination of
an SEM image taken after the analysis will show a spot where the probe had
been (in the STEM/TEM the contamination is usually very obvious).

It can be very difficult to predict when you will see contamination
problems, and when you won't. Even two samples prepared, supposedly, in
the same way, and examined one after the other, may behave differently.

Tony G-R

At 12:24 PM 12/13/2001 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
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** Cambridge, MA 02139-4307
** USA
**
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** Fax: (+) 1-617-258-6478
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From daemon Fri Dec 14 09:06:26 2001



From: Susan Stanton :      stantosg-at-email.uc.edu
Date: Fri, 14 Dec 2001 10:08:34 -0500
Subject: LM experience with Microgen Optics

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I am considering purchasing a refurbished fluorescence microscope from
Microgen Optics. Any feedback re: experience with this company would be
much appreciated. Thanks,

Susan Stanton, Ph.D.
College of Allied Health Sciences
University of Cincinnati
Cincinnati, OH 45267-0394



From daemon Fri Dec 14 09:36:47 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 14 Dec 2001 09:25:34 -0600
Subject: RE: EDS analysis / "Chemical shift"

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Chemical shift (changing of the position of the peak due
to changing energy of the outer electrons because of the
chemical interaction) for fluorine should be relatively small.
It usually important only for WDS acquisition if it is performed
on standard position. For EDS it should be irrelevant since
the whole spectra is acquired and chemical shift will
affect mostly position and not intensity of a peak.

I think you run into one of the two (or both) problems:
1. Beam induced migration of fluorine (most probable).
2. Carbon contamination under the beam.

You can try to apply methods suitable for beam sensitive
specimens.
- Use only low magnifications for very fast observing
of the specimen.
- Make all preparations at higher magnifications -
focusing and whatever else - on one place, then move to
desired place and perform acquisition as quickly as possible.
- Defocus beam as much as possible.
- Use shorter acquisition times and lower voltage and
beam current.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: Oakley, Jeff [mailto:oakleyj-at-rayovac.com]
} Sent: Thursday, December 13, 2001 12:25 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: EDS analysis / "Chemical shift"
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} I am currently working on an EDS analysis project on carbon / fluorine
} matrices. Usually carbon starts as the taller peak, and remains so
} throughout the acquisition. Occasionally, however, when starting an
} spectrum acquisition I will see the fluorine peak start as
} the taller peak,
} then slow to become the shorter peak.
}
} I was told by an observer that there is a phenomenon known as
} "chemical
} shift," that could have an impact on the amount of certain
} elements in the
} interaction volume of a sample. This person didn't know much
} about it other
} than it could give you inaccurate quant results.
}
} Is there such a thing as chemical shift, and if so, could
} someone explain
} what exactly is happening during the process?
}
} Thanks,
}
}
} Jeff Oakley
} Rayovac Corporation
}
}
}


From daemon Fri Dec 14 11:31:25 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 14 Dec 2001 09:23:24 -0800
Subject: RE: IC cross sections -- with high Z contrast

Contents Retrieved from Microscopy Listserver Archives
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Mark and others:

Lots of good suggestions. Several questions I did not
answer. First, the SEM is an AMRAY 1910 Schottky
FESEM. Over a reasonable condenser setting range,
the probe diameter is about 90A at high condenser
setting, 10KV. I have a new Keithley picoammeter
on order to use to re-do the probe diameter measurements
and record specimen currents. My DMM approach
does/did not work.

The cross section sandwich is non-conducting by its nature.
Since the device is a completed IC, it has all underlayers, poly,
interlayer dielectric, double metal interconnect, 2KA SiO2
and 10KA SiN passivations. Top down, this device would charge.
I can image it at moderate KV and small probe diameter with
BSE.

My BSE is a Robinson Model 6. I can mix any amount of
SE and BSE in any relationship. SE+BSE, SE-BSE, BSE-SE,
etc.

My scope has a Gamma control. However, it does not work.
This could be because I'm not using it correctly or have not even
turned it on. I will check this for sure. If it is broken, Amray
will fix it. Gamma is a good idea to try.

The other idea of separate images of different Z areas of
interest has potential. How different this would be from the
mixer on the Robinson remains to be seen. Even so, separate
images could be shot and aligned afterwards.

Procedurally, the cross sections are made with traditional
Buehler equipment and fine diamond paste polish. Specimens
without barrier metal turn out quite well. This new lot with
TiW is a real problem. The next one has TiN barrier metal.
The TiN devices are built on thin SOS. The ones I'm working
with now are bulk CMOS. The TiW devices will shift to SONOS
early next year.

The consensus seems to be that coating is a waste of time.
I can't really argue with that, based on my bad prior history
of doing this. I have a new specimen with TiW which is
mounted and desiccated overnight. I will try looking at it
without any coating. The main glass slide is stuck down
on a 90 degree Pella stub and the bottom portion is covered
with coloidal silver. The upper part of the specimen is
un-touched.

Thanks to all.
gary g.


At 03:59 AM 12/14/2001, you wrote:
} You didn't mention what type of SEM you are using. I assume that you are
} looking at cross-sections of the IC's. We use a FESEM and use 2.5kv and
} that works nicely without any coating at all. Before we had the FESEM we
} used a gold coater. We did not coat the cross-section directly but laid it
} flat down so the gold just drifted over the edge of the cross-section. This
} gave us very nice contrast with the metalization and the SiO2. Let me know
} how this works for you or give me a call.
} Mark
} 612-954-2845



From daemon Fri Dec 14 14:16:44 2001



From: mega_star7tgt7-at-excite.com
Date: Wed, 12 Dec 2001 23:50:16 -0500
Subject: .....improve reception 23 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Poor reception and dropped calls getting you down?
Get the cell phone booster antenna.
Easy to install works on any cell phone


http://61.16.69.50/booster



Removes to: CellSales4-at-excite.com With "PsRemove" in the subject














Poor reception and dropped calls getting you down?
Get the cell phone booster antenna.
Easy to install works on any cell phone













From daemon Fri Dec 14 17:06:21 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 14 Dec 2001 14:58:12 -0800
Subject: High Z cross sections

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Thanks to all for the many excellent suggestions.
Some ideas had already been tried while others
had not.

Gamma is a good idea. My scope has gamma feature
but for some reason, it does not work. A service call
should solve that problem. But I found a very nice way
to handle the huge Z contrast range in barrier metal
ICs.

I still have to coat the specimen with only about 30A of
Au/Pd or Pt to eliminate charging. I shoot at between
5-10KV with medium spot size. At this range, the key
is to do a SE-BSE image with the amount of subtracted
BSE set for best image. This seems rather obvious right
now. But it did not this AM.

Setting up the SE for excellent detail and definition in the
low Z areas while ignoring the high Z areas is the first action.
Then, engage mixing, set for SE-BSE and adjust the amount
of SE to BSE so that the overall contrast range is evened out.
The results are perfect, I think.

you can see a sample at:

http://photoweb.net/Test7-5dsp8bc.jpg

If anyone has any other ideas or suggestions, please don't
hesitate to put them forward.

Thanks,
Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA
916.791.8191
916.791.8186 fax



From daemon Fri Dec 14 17:12:39 2001



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Fri, 14 Dec 2001 15:13:11 -0800
Subject: call for image processing presentations

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Howdy all

Start off the New Year with plans to attend the annual MSA/MAs
meeting in Quebec August 4-8 2002. This year, the Education Outreach
symposium will be examining the use of image processing as a tool in
the academic curriculum. In addition to using image processing as a
way to increase the use of microscopic images in classes, Ken and I
are interested in new approaches for microscopy or remote access to
expand the role of microscopy and microscopes in the classroom at any
level.

If you are interested in presenting in this symposium, please contact
me. I have included the symposium abstract for your information.

Please note: we are more interested in discussing image processing
as a curriculum tool than in discussing 'how to' process images.

See you all in Quebec
Steve Barlow


Teaching and Learning - Creating Effective, Innovative Solutions in
Microscopy, Imaging, and Analysis
Steve Barlow and Ken Baker

Teaching and learning in the field of microscopy imaging and analysis
are important to the practice of all forms of microscopy. Microscopy
imaging and analysis has adopted an extensive collection of tools and
techniques and continues to develop as an increasingly complex
subject area. Computer-assisted microscopies, high speed global
internet communications, remote microscopies and image acquisition,
digital imaging tools, and multidimensional imaging and analysis
techniques all offer opportunities for powerful innovation in
teaching and learning. Pedagogical resources include: distance
learning, on-line manuals, references, tutorials, image databases,
and a selection of shared utilities and dedicated discussion forums.
This symposium will encourage contributions that emphasize creative,
effective, and innovative educational approaches to microscopy, image
acquisition and analysis, and increased use of microscopy and images
in a student curriculum.


From daemon Fri Dec 14 17:14:10 2001



From: Vr. Richard Bejsak-Colloredo-Mansfeld :      ricardo-at-ans.com.au
Date: Sat, 15 Dec 2001 10:09:46 +1100
Subject: looking for nice photo of beetle

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dear colleagues
I would like to add anatomy of the beetle. I am looking for nice scan
picture of the beetle both sides...

Thank you very much for your help.

Marry Christmas

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Colloredo-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).




From daemon Fri Dec 14 20:21:31 2001



From: Bill & Sue Tivol :      wtivol-at-earthlink.net
Date: Fri, 14 Dec 2001 18:12:23 -0500
Subject: Re: EDS analysis / "Chemical shift"

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on 12/14/01 6:04 AM, Allen Sampson at ars-at-sem.com wrote:

}
} As mentioned
} by another poster, 'chemical shift' in x-ray spectroscopy refers to the
} actual shifts in peak locations caused by chemical bonds altering the
} quantum energy levels of the electron shells. This effect will only be
} seen on fully focusing Johannson wavelength spectrometers or the more
} recent microcalorimeter systems, developed at NIST.
}
In the interest of completeness, the tunnel junction diode detector,
also recently developed, has energy resolution in the few eV range, so it is
also able to detect chemical shifts.
Yours,
Bill Tivol



From daemon Sat Dec 15 09:32:17 2001



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Sat, 15 Dec 2001 09:20:59 -0600
Subject: Re: TEM coccolithophorids

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Hi Jonathan,

I have no experience with TEM investigation of coccolithophorids, but for
SEM, the folks here spray them onto stubs - they're so tiny they adhere on
their own - and then sputter coat. Also, filtering seawater with coccos in
it and looking at a cutout from the filter paper (in the SEM, at least)
works well for us.

Good luck,
Dee
}


} Season's Greetings:
}
} I am looking for anyone with experience looking at coccolithophorids using
} TEM.
}
} We need to look at sections. The guys are only about 5 um in diameter. I
} have done some thin layer embedding and individual cell selection before,
} but never on anything this small. I will be checking other sources of
} information about techniques, but thought I would ask here too,
}
} Any hints or tips on fixing, embedding, etc. that might help?
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu



***************************************************************
Please do not publicly post any of my correspondence without permission

Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 845/365-8640
F: 845/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)


From daemon Sat Dec 15 09:32:22 2001



From: Mary McKee :      mckee-at-helix.mgh.harvard.edu
Date: Sat, 15 Dec 2001 09:21:35 -0600
Subject: anti-fade agent

Contents Retrieved from Microscopy Listserver Archives
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Dear Listmembers,

Does anyone know of an antifade agent that works for liver-cell microscopy
systems? We're having trouble with fading of the fluorescent signal.
Thanks in advance.

Mary

Mary McKee
MGH Renal Unit
Charlestown, MA 02129
(617)726-3696


From daemon Sun Dec 16 00:38:17 2001



From: Alan Eugene Davis :      adavis-at-saipan.com
Date: Sun, 16 Dec 2001 16:23:44 +1000
Subject: (long) SUMMARY: responses to "artifacts and blunders" request

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Patient list:

I am posting a summary of responses to my request to this list on 3
December 2001, for information about any roles microscopic artifacts
may have played in the history of biology. I posted my own experience
regarding Sidney Hickson's description of reproductive events in
_Millepora_ sp)p). (A copy of my somewhat lengthy post is enclosed at
the end of this summary.)


To be fair to Sidney Hickson, who drew and described the incredible,
imagined sequence of reproductive events in Millepora sp(p). I
mentioned in my original , I believe that he did print a retraction of
the findings in that paper.


Gareth Morgan (whose contribution is mentioned below) offered the
following remark:

We tend to see what we expect might possibly be there and
consciously or unconsciously ignore the rest as 'noise' - perhaps a
natural action but also potentially dangerous.

Maureen A. Peterson offered a similar remark, which I took as
personal advice:

"Lesson? I better keep my eyes and my mind open."





Both Volker Brinkmann and Mike Dalbey called attention to the
"homunculus" (little man-shaped pre-embryos) which were reported in
the 18th Century in human sperm.


Volker Brinkmann:

The first microscopic misinterpretation was probably that by Antoni
van Leeuwenhook analysing human sperm. He was convinced to see
little men with heads, arms and legs swimming around. There are
some nice drawings of that.


Mike Dalbey:

Surely the most famous misinterpretations of microscopical
observations are those recorded in drawings made by
preformationists in the 18th Century of the "homunculus" in the
head of human sperm. Several of these drawings are commonly
reprinted in textbooks as cautionary examples.


Mike Dalbey further pointed out several interesting examples,
including a contemporary issue, the putative bacteria fossiles in the
martian meteorite::

For a discussion of a common histological artifact see
"Multinucleate Plant Cells" by Burkholder and Mc Veigh (1941) in
vol. 68 of the Bulletin of the Torrey Botanical Club p. 395.

You should also check out what the web has to offer on the history
of Royal Rife and the Rife Microscope.

Finally, evidence seems to be accumulating that the "nanobacteria"
observed by EM in the martian meteorite are artifacts.


Maurreen A. Peterson (Mape-at-mail.ifas.ufl.edu) posted concerning a
disease of grapes, a case of failure to see the etiological agent,
which was there all the time:



Being a Plant Pathologist, the best I can think of is the story of
Pierce's disease of grapes, which was of unknown etiology for a
long time. Forgive me for not seeking details from the literature.

At one time it was proposed to be caused by a virus. Extensive
light microscope work missed the true cause- a bacterium. After
elucidation of the pathogen, I am told that upon review of work
previously done, the bacterium WAS there to be seen, but was
missed.

Upon a similar vein, MLO's in plants were not recognized in TEM
work until a human or animal pathologist saw micrographs of MLO's
in plant tissue, and was readily able to say what they were.

Anecdotal, but food for thought.



Two similar misinterpretations of existing evidence were pointed out
by Gareth Morgan ( {Gareth.Morgan-at-impi.ki.se} ). The first regarded
_Helicobacter pylori_, a causitive agent for gastric ulcers.


Not sure if this one fits the bill but it is a similar one to the
grape disease story by Maureen Petersen.

The one I mean is the discovery/description of Helicobacter pylori
in human gastric biopsies in the early 80's (when it was given the
name Campylobacter pylori). It had been there all of the time but
had been disregarded as 'stuff'.

Gareth suggested a google search might reveal more information, and
indeed it did: over 82,000 hits. I hope I will be forgiven for
quoting one site in part:

Although they were the first to succeed in establishing a link
between bacteria and ulcers, the Australians were not the first to
try. Since the time of Robert Koch in the late19th century,
microscopists observed curved bacteria among the cells under the
mucus lining of the stomach, particularly in and around ulcer
craters. No one had ever succeeded in isolating the microorganisms,
however, and their presence had been explained away as artifact or
postmortem contamination. Besides, it was thoroughly accepted among
clinical microbiologists of the time that it was unlikely that
bacteria could live and grow in the strongly acidic environment of
the human stomach.

Warren, a pathologist who examined gastric biopsies, also observed
the curved rod-shaped bacteria under his microscope. After
examining many such specimens, he realized that the bacteria were
always present in tissue that showed signs of inflammation, that
the number of organisms correlated with the degree of the
inflammation present, and that they occurred in half of the routine
gastric biopsy specimens he examined. Convinced that his
observations were significant and merited further investigation, he
kindled the interest of Barry Marshall, then a trainee in internal
medicine, and together they set out to isolate the source of the
infection.

[This site is: http://www.faseb.org/opar/pylori/pylori.html]


Gareth also suggested a similar misreading may have been involved with
"the finding of Actinomyces in cervical smear material." I was unable
to find any clear elucidation of such events; however, one site
mentioned that care should be taken by the pathologist in diagnosing
"atypical _Mycobacteria_" due to possible presence of Actinomycetes.




H. F. Moura Nunes posted about another possible case:


I am not sure of that. But I heard some years ago that the first
information about the structure of poliovirus - in the early days
of the electron microscope - was that they were long filamentous
structures. Some time later it has been seen that the filamentous
structures were in fact bacterial flagella.




Tina Carvalho posted a long discussion of a personal experience
regarding another real structure that had been interpreted as an
artifact. The experience must be re-posted in its entirety, in spite
of its length.


Although I 'm sure there are examples of misinterpretation of
artifacts that have been perpetuated for many years, I can offer
here a story of the flip side. This is about what appeared to be a
well-known artifact that turned out to be, in fact, a surprising
real structure. It's also an embarrassing personal story!

An undergraduate student was working with some colleagues who were
studying the behavior and neurophysiology of the escape response of
several species of planktonic copepods (small crustaceans). They had
reams of data and were beginning to characterize the responses of
the animals to different kinds of stimuli. Each species had it's own
characteristic response, but a general picture was beginning to
form. There appeared to be two different classes of response though,
one type particularly puzzling. The undergrad spent some time in
the EM facility with me, using SEM to describe the sensory
setae. She expressed an interest in trying TEM and so we went
through fixation, embedding, sectioning, etc. I had worked on the
tiny beasts for close to 10 years and had found some interesting
structures that I worked on from time to time. Copepods were
difficult to fix, some species more so than others. So I had
concentrated on the "pretty" ones and temporarily given up on the
"ugly" ones - the ones with all those myelin body artifacts that I
just couldn't seem to avoid. Finally it was time to try to work on
them again, so I gave this one particularly difficult species to the
undergrad to try to fix and section. She came to me with
micrographs, wondering what in the world were those squiggles she
saw. I patiently explained that myelin bodies were a fixation
artifact, produced when lipids became mobile during fixation, then
reformed in these "onion bodies" . I sent her back to work to try
again, and she came up with the same results. "What if they aren't
an artifact?" she kept asking. I explained that invertebrates do not
have such membranous structures; they were artifactual. I brought
out the books and papers about myelin body artifacts. I brought out
the books and papers that said invertebrates do not have membranous
wrappings around their axons. She was unconvinced. In a hurry one
day when she was really bugging me I dug through my files and found
the decade-old folder to show her I'd seen the same thing - an
artifact. "But there's an axon in the middle of each one", she
protested, not knowing that was "impossible". I glanced at them
impatiently and saw that she was right, but she was running off to
class.

Later that day we looked closely and decided that, indeed, the
images were a real mess and difficult to interpret, but there was a
faint possibility that these weren't classic myelin body artifacts.
Whatever it was was certainly reproducible, and these forms appeared
every time in the antennal nerve. So we emailed the PIs of the
project and told them we might have myelinated axons in the
difficult-to-interpret species. It was April1, April Fools' Day, the
undergrad's name was April, and they thought it was a joke. After
all, the dogma is that invertebrates don't have myelin! Check any
biology book.

But by the next day they realized that myelin (OK, "myelin-like
structures" for you purists) would instantly and completely explain
10 years' worth of puzzling data.

It took awhile to prove to ourselves (mainly me, since I'd been
repeating the dogma for many years) that these structures were real,
orderly, and extensive wrappings of membrane around the axons. It
took ultrarapid cryofixation and cryosubstitution to really show
that the artifactual bodies that had plagued a number of people
working on these critters were in truth these surprising,
dogma-busting structures.

We made the April the first author on the Nature paper, which came
out in April 1999.

I don't take anything for granted anymore! The lesson is: keep an
open mind.

For more on the copepod story- Copepod neuroecology
http://www.pbrc.hawaii.edu/~petra/copepod.html



Finally, perhaps appropriately, I include my original posting:



I am interested in gaining insight into the role of microscopic artifacts
in the history of biology. May I impose on list members to contribute
particularly glaring examples of misinterpreation of biological facts due
to improper microscopic technique? I apologize if this is off-topic or a
waste of bandwidth.

Let me provide the first example of a misinterpretation and request your
assistance in learning whether this was due to improper use of the
microscope, or perhaps even malfeasance: Sidney Hickson's early work on
Millepora spp. (Cnidaria:Hydrozoa) fire corals. It seems an incredible
lapse, a wholly fabricated natural history account, one that persisted for
a considerable period in the fabric of the mythology of biological
knowledge. I am interested because reproduction of Millepora platyphylla
is the subject of incompleted thesis research of mine.

Hickson published a report on reproduction of "Millepora" around the end
of the 19th Century. (Anong his other errors he synonomyized all species
of Millepora as ecomorphs of one, M. alcicornis.) In this report, which
I do not have available at this time, he included several plates of
drawings depicting a putative sequence of reproductive events in this
organism. We now understand that his depiction is not even close to the
way that Millepora spp. (which were later redesignated as proper
individual species through painstaking work by Boschma---notwithstanding
the issues recently raised by molecular work) reproduce. The depiction
involved dozens of drawings, and a sequence of events based on a
misinterpretation of what are apparently artifacts.

Hickson (of Cambridge University) worked extensively in the field,
including Indonesia and the Philippines. Was his microscopic work done
in the field? Are members of this list enlightened as to Hickson's
methods?

Hickson's erroneous drawings of the medusae of Millepora lived on for over
3/4 of a century in virtually every Invertebrate Zoology textbook
published until the late 1980s or 1990s. His erroneous description of the
medusa of Millepora as lacking a velum led to the designation of a
separate branch of hydromedusae by Mayer, as the only hydrozoan medusa
without a velum. My unpublished observations in the 1980s as well as
published observations by John Lewis of McGill University showed that the
medusae of Millepora spp. clearly possess a velum.

I apologize for monopolizing the bandwidth. I hope this is as fascinating
a topic for others as for myself, and not considered off-topic.






--
Alan E. Davis, Science Instructor
Marianas High School
PMB 30, Box 10006,
Saipan, MP 96950
Northern Mariana Islands
adavis-at-saipan.com


"An inviscid theory of flow renders the screw useless, but the need
for one non-existent."
---Lord Raleigh(aka John William Strutt),or else
his son, Jr., who was also a scientist.



From daemon Sun Dec 16 01:15:17 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 15 Dec 2001 23:09:12 -0800
Subject: Re: Fw: Joysticks

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I'd be willing to work on them at $75 plus parts.
Most of the problems are cleaning of the stick
and guides. Other problems are dirty pots.

Send 'em to me and I can fix them and send them
back.

No guarantee, but its worth a shot.

gary g.


At 03:40 AM 12/12/2001, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Dec 16 18:55:55 2001



From: Keith Cochran :      Cochrak-at-egr.uri.edu
Date: Sun, 16 Dec 2001 19:41:06 -0500 (EST)
Subject: AFM Help

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I am an undergraduate ChemE major at the University of
Rhode Island and I had a question on techniques and AFm
capabilities.

Is there a way to measure the force a piezoelectric
crystal applies with a certain voltage using an AFM and
proscan version 1.5b.0001

Right now I can use the cantiliever to exert force and
measure the spring constant of a sample by pushing
down, using spectroscopy.

Can it be done without pushing the cantilever down, and
instead put a voltage though the crystal and have the
crystal push up and measure the force exerted?

Also, on a side note, what is the best way to prepare a
blood sample onto a slide (or what would be better than
a slide) in a study on the aging of blood. This study
will be using primarily AFM techniques, and perhaps
nanoindentation. It will also be using the same
samples for several weeks, perhaps monthes. After only
a week the samples are already crumbling. How can I
keep them at ambient conditions, and still keep them
from breaking apart?

Thank you,
~Keith Cochran


From daemon Mon Dec 17 10:20:36 2001



From: Pradyumna Prabhumirashi :      p-prabhumirashi-at-northwestern.edu
Date: Mon, 17 Dec 2001 10:04:23 -0600
Subject: TEM Sample Prep

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Hello listers:
I am currently trying to prepare TEM samples of polycrystalline
strontium titanate (grain size ~20 micron) by conventional dimpling/ion
milling. However, due to porosity, I have been having problems in
thinning down this material. One of the suggested sample prep methods
was to thin the material up to say ~50micron and then embed some sort of
epoxy in it, so that the epoxy will fill in the pores.
Could someone explain to me how to do this? What type of epoxy should I
use and how do I ensure that the epoxy has filled up the pored?
Thanks,
Prad

Pradyumna Prabhumirashi
Dept. of Materials Science
Northwestern University
Phone: (847)491-7798
Fax : (847)491-7820




From daemon Mon Dec 17 14:23:51 2001



From: mancini :      mancini-at-bcm.tmc.edu
Date: Mon, 17 Dec 2001 14:15:30 -0600
Subject: Postdoc opening at Baylor College of Medicine

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Postdoctoral Associate

A postdoctoral associate position is immediately available in the Department
of Molecular & Cellular Biology, Baylor College of Medicine, Houston, TX.
The work focuses upon steroid receptor function in an integrating molecular,
biochemical, genetic and cell biological approach, and is extremely imaging
intensive (including both live and fixed cells, LM/EM). Specific interests
address questions of the relationship between transcription factor function,
subnuclear organization, intranuclear dynamics and associations with nuclear
structure. This single cell approach also allows examining in vivo
protein-protein interactions, large-scale chromatin organization, and direct
transcription read-out using cell lines with integrated bioluminescent
reporters. Imaging resources include state-of-the-art confocal and
deconvolution microscopy, and a new digital transmission electron
microscope. All microscopes and robust computer/software resources are
housed in a completely redesigned/renovated Integrated Microscopy Core. The
ideal candidate will have extensive molecular/biochemical experience, OR,
extensive imaging/computer training. The ability to communicate
exceptionally well (oral and written) and interact with a larger group
studying mechanisms of receptor function from many other angles is
imperative.

Interested applicants should send an electronic CV and a cover letter
expressing their research interests and career plans to:

Michael A. Mancini, Ph.D.
Assistant Professor
Director, Integrated Microscopy Core
Department of Molecular & Cellular Biology
Baylor College of Medicine
Houston, TX 77030
713.798.8952
mancini-at-bcm.tmc.edu
http://microscopy.bcm.tmc.edu
web version of the ad: http://208.188.94.28/templates.asp?PageID=127


References:

Stenoien DL, Nye AC, Mancini MG, Patel K, Dutertre M, O'Malley BW, Smith CL,
Belmont AS, Mancini MA. (2001) Ligand-mediated assembly and real-time
cellular dynamics of estrogen receptor alpha-coactivator complexes in living
cells. Molecular and Cellular Biology. 21:4404-12.

Stenoien DL, Patel K, Mancini MG, Dutertre M, Smith CL, O'Malley BW,
Mancini MA. (2001). FRAP reveals that mobility of oestrogen receptor-alpha
is ligand- and proteasome-dependent. Nature Cell Biology. 3:15-23.

Stenoien DL, Mancini MG, Patel K, Allegretto EA, Smith CL, Mancini MA.
(2000). Subnuclear trafficking of estrogen receptor-alpha and steroid
receptor coactivator-1. Molecular Endocrinology. 14:518-34



From daemon Mon Dec 17 14:58:11 2001



From: Karli Fitzelle :      fitzelle.1-at-osu.edu
Date: Mon, 17 Dec 2001 15:51:29 -0500
Subject: Fixing petunia petals..........................

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Hello everyone!!

One quick question.....I am currently performing a general TEM fixation on
petunia petals. In our lab, we place plant tissue (once dissected and in
fixative) in a vacuum to remove the air. This normally causes the tissue to
sink. For these petals, however, I have had them in a vacuum 5 times so far
(around 15 minutes each time, or until they "bubble over") and some of them
are still not sinking..........

It's almost as if they are hydrophobic. Does anyone know if there is
something about petals in general that makes them more difficult to
infiltrate with the fixative? I am doing leaves as well and they sank to
the bottom just fine.

Does anyone else on this list perform a lot of plant tissue (leaves /
petals) fixation? If so, do you place your samples in a vacuum, or use a
detergent like Triton X to get the tissue to sediment?

I'm wondering how much damage is caused by placing the tissue in a vacuum
as opposed to using a detergent. I know the detergent disrupts membranes,
but wouldn't a vacuum have the same effect? Are there other means to get
the tissue to sediment? Is one technique better than the other?


Any input would be greatly appreciated!

Thanks in advance



From daemon Mon Dec 17 15:16:07 2001



From: James Martin :      james.s.martin-at-att.net
Date: Mon, 17 Dec 2001 16:10:05 -0500
Subject: microchemical tests for fecal matter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am helping a museum establish a protocol to identify residues on African
tribal beads, which is thought to be fecal matter. The sample material will
be limited to micrograms and will contain mineral and other contaminants.
Can anyone recommend microchemical or micro tests that might be used to
differentiate fecal matter?

With thanks,

Jamie



From daemon Mon Dec 17 18:01:47 2001



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 17 Dec 2001 18:53:58 -0500 (EST)
Subject: Re: microchemical tests for fecal matter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If the material is dried out, the bugs will probably be dead and
uncultivable. Microscopy observation, unless there are specific
antibodies against enteric bacteria available for labeling, will be
useless. Probably the best test would be a molecular test for E. coli
(e.g., PCR).

On Mon, 17 Dec 2001, James Martin wrote:

} Date: Mon, 17 Dec 2001 16:10:05 -0500
} From: James Martin {james.s.martin-at-att.net}
} To: microscopy-at-sparc5.microscopy.com
} Subject: microchemical tests for fecal matter
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am helping a museum establish a protocol to identify residues on African
} tribal beads, which is thought to be fecal matter. The sample material will
} be limited to micrograms and will contain mineral and other contaminants.
} Can anyone recommend microchemical or micro tests that might be used to
} differentiate fecal matter?
}
} With thanks,
}
} Jamie
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Mon Dec 17 19:17:57 2001



From: R. Ann Bliss :      bliss5-at-popcorn.llnl.gov
Date: Mon, 17 Dec 2001 19:05:49 -0600
Subject: JEOL 733 Help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy Holidays:

I have gained the above mentioned microprobe, but it doesn't work
yet. It was disassembled in another building, brought over and I have
reassembled it to the best of my ability. Kind of a baptism by fire.

The chiller is chilling and the water is coursing around the oil
reservoir as well as into the appropriate parts under the column. The
oil, used to cool the objective lens, is running from inside that
reservoir through new hoses. And the vacuum measures in the 0-5 micro
amp range where I'm told it should be (its actually 0.3 at the gun
and 0.5 in the column). And the penning gauge measures in the 10^-5
range at the main evacuation manifold - that's good, too.

There is a gauge (Load/Filament) on the Accelerating Voltage panel
that should light up "when the vacuum is at the desired level"
according to the manual. But something is not allowing that to
happen. Consequently, the high voltage cannot be turned on. And there
is the rub.

We do not have a service contract on this beast yet. I have called
the service people and they have given me a considerable amount of
help to date. We just can't get any farther than this. Is there any
help for me and my albtross?

Kind regards,
--

+++++++++++++++++++++++++++++

R. Ann Bliss
Technician, Chemistry and Materials Science
Materials Science and Technology Division
Lawrence Livermore National Laboratory

_____________________________


From daemon Mon Dec 17 22:27:13 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 17 Dec 2001 20:19:25 -0800
Subject: Re: microchemical tests for fecal matter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please let me give you a perspective from that of
a photographer. In this respect, I have poop specimens
which are between days and years old. They all seem
to share the same basic characteristics. There is
fiber, bulk, stuff, and lots of E. coli. I have also found
a few cocci in the mess.

These specimens were sealed under #1 cover slips
with cyanoacrylate glue. They are still viable to this day.

The specimens I made for SEM were not very sophisticated.
I would have fixed them with Osmium and done CPD. I am
not set up to do this. But what I found was that the bacteria
were crushed by the high vacuum of the SEM....but they were
still viable, morphologically. But without fixing, they deteriorate
rapidly. Even stored at 40mT, they do not hold up. Being
devoid of Oxygen, the bacteria seem to be confused--as
I have seen nearly zero spores. Only dead rods. Of course,
there may be other organisms present which do not sporulate.
Anyway, not many spores are seen.

It seems to me that the standard protocol for bacterial fixation
is quite good and certainly appropriate. In contrast, the LM
method can go on for a long time. But of course, the magnification
and resolution between LM and SEM is quite different.

But...to your problem. Given that you have a very small amount
of material with which to work, experimentation is not a luxury.
I would suggest taking a small amount of material and doing a
gas chromatograph analysis on it and then taking another small
amount and examining it under SEM. With the SEM of the material,
you will probably see a lot of crap that is not at all associated with
the human crap (feces). What I mean is, junk. This will take some
doing to evaluate. Sorting it all out will not be easy at all.

Lots of luck.

gary g.



At 01:10 PM 12/17/2001, you wrote:

} I am helping a museum establish a protocol to identify residues on African
} tribal beads, which is thought to be fecal matter. The sample material will
} be limited to micrograms and will contain mineral and other contaminants.
} Can anyone recommend microchemical or micro tests that might be used to
} differentiate fecal matter?
}
} With thanks,
}
} Jamie



From daemon Tue Dec 18 03:57:09 2001



From: simon gary :      simongary-at-onebox.com
Date: Tue, 18 Dec 2001 02:40:03 -0800
Subject: URGENT AND CONFIDENTIAL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Keith

I would recommend you ask this question to the SPM mailing list
http://www.di.com/spm%20forum/spmforummailinglist.html

You may also find the information you need in the application notes found at
the DI and Thermomicroscope websites (they are now the same company but
still have separate websites): - www.di.com and http://www.tmmicro.com/

Giles

***************************************************************************

Dr. Giles H.W. Sanders

DeltaDOT Ltd.
PFSG group
ACE Building
Department of Bioengineering
Imperial College
London
SW7 2AY

0207-594-5174
0794 - 1312335


www.deltadot.com



Information on scanning probe & microfluidics : www.achem.ic.ac.uk/gsanders
****************************************************************************
*******
****************************************************************
----- Original Message -----
} From: "Keith Cochran" {Cochrak-at-egr.uri.edu}
To: "Microscopy Listserve" {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, December 17, 2001 12:41 AM


Karli
Yes, petals are very often hydrophobic. They are covered with a waxy,
hydrophobic cuticle.
The cell surfaces are very often papillate and the papilla surface may
be minutely wrinkled, increasing the water contact angle so that the
surface is non-wettable. The most important thing (and this applies
to fixation of many types of plant leaves too) is that the pieces you
are fixing are small enough for the glutaraldehyde fix to penetrate
rapidly via the cut edges - it will penetrate only exceedingly slowly,
if at all, via the cuticle, so total immersion is not worth striving
for. Cutting pieces 1/2 (half) to 1 mm square should be the aim. Do
this using a slicing action with a very sharp degreased razor blade
with the petal in a pool of glutaraldehyde in a petri dish, so that
cut surfaces are immediately exposed to fix. Subsequent vacuum
infiltration will help to get the fix into the tissue if the petals
(or leaves) have a lot of intercellular air space, but they will
continue to float.

Chris

----- Original Message -----
} From: "Karli Fitzelle" {fitzelle.1-at-osu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, December 17, 2001 8:51 PM





I am DR Nelson Afam {a Civil servant in the
ministry of Health} here in Nigeria. I know this
letter would come to you as a surprise because we have
not met before either physically or through
correspondence. I got your contact from our chamber of
commerce here in Nigeria and have no doubt in your
ability to handle this proposal involving huge sums of
money.

The Subject: My father chief Michael Afam {Now late}
was the Royal head of my community, Eleme {an oil
rich town} in Nigeria. My community produce 5.8% of
the total annual crude oil production in Nigeria and
0.5% of the Dollar value of each barrel is paid to my
father as royalty by the federal government. In his
position as the royal head and chairman of the Akwaeze
oil trust fund, he made some money which he behind for
his children. The money is twenty five Million, Six
thousand US Dollars {$25 .6m}. This money originated
from the accumulated royalties between 1998.

Due to poor banking system in Nigeria and
political\economics instability as a result of past
military rules {1985-1999}, he deposited this money in
a “Box” with an open beneficiary in a local security
firm. He told the security Company that the content of
the box is Gold worth $25.6m. And the box will be
safeguarded until he finishes arrangement to transfer
it abroad. He was planning this when he died last year
of heart Attack.


The Proposal: Just before my father died, he called
my attention in confidence to the money and charged me
to look for a foreigner who would assist me in the
transfer\investment of the fund. So I would be very
grateful if you could accept to help me achieve this
great object. I promise to give you 20% of total fund
s transferred as compensation for your assistants.
Five present {5%} would be set aside to take care of
all expenses we may incur during the transaction. To
the indicate your interest, contact me urgently and
confidentially through E-mail for information and the
roles you will play in the business. God
bless you.
DR Nelson Afam






__________________________________________________
FREE voicemail, email, and fax...all in one place.
Sign Up Now! http://www.onebox.com



From daemon Tue Dec 18 08:18:24 2001



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Tue, 18 Dec 2001 09:08:47 -0500
Subject: Re: EDS analysis / "Chemical shift"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Jeff-
I do not know what beam energy you are using, but another possibility
is that the sample(s) in question are charging enough to reduce the
effective landing energy of the primary electrons thus altering the peak
ratios. I would be surprised if this is what is occurring in your case
since the C and F peaks aren't that far apart in energy, but fluorocarbons
can be very insulating. With my PGT EDX system in mapping mode, I can view
the quasi-real-time count rates in the C and F energy windows. Perhaps if
your system can do the same, that could shed some light on your problem
(e.g. are both C and F signals getting smaller with the F signal doing so
faster, or is F going down as C is going up?). Are the samples gold
coated? Does gold coating eliminate the observed effect? Good luck.

Sincerely,
Matthew Ervin, Ph.D.
(301)394-0017 phone, (301)394-1559 fax
MErvin-at-ARL.Army.mil

M/S: AMSRL-SE-RL
US Army Research Laboratory
2800 Powder Mill Road
Adelphi, MD 20783-1197

Disclaimer: The opinions and views expressed above are those of the author
and do not necessarily represent those of the U.S. Army Research Laboratory
or any other government agency



"Oakley, Jeff"
{oakleyj-at-rayovac.com} To: "'Microscopy-at-MSA.Microscopy.Com'"
{Microscopy-at-sparc5.microscopy.com}
12/13/01 01:24 PM cc:
Subject: EDS analysis / "Chemical shift"






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am currently working on an EDS analysis project on carbon / fluorine
matrices. Usually carbon starts as the taller peak, and remains so
throughout the acquisition. Occasionally, however, when starting an
spectrum acquisition I will see the fluorine peak start as the taller peak,
then slow to become the shorter peak.

I was told by an observer that there is a phenomenon known as "chemical
shift," that could have an impact on the amount of certain elements in the
interaction volume of a sample. This person didn't know much about it
other
than it could give you inaccurate quant results.

Is there such a thing as chemical shift, and if so, could someone explain
what exactly is happening during the process?

Thanks,


Jeff Oakley
Rayovac Corporation






From daemon Tue Dec 18 08:29:04 2001



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 18 Dec 2001 09:25:57 -0500
Subject: Re: Fixing petunia petals..........................

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karli,
This primary fixative worked very well for Arabidopsis
inflourescences even the smallest buds. I also used it for fixing bean
(Fava) leaves but in that case I needed a brief vacuum to submerge the leaf
samples. The reference is included.
2.8 % glutaraldehyde
Buffer 0.1 M Hepes buffer (pH- 7.2) + 0.02% Triton X-100
Buffer wash 0.1 M Hepes buffer

Owen, Heather A., and C.A. Makaroff, Ultrastructure of microsporogenesis
and microgametogenesis in Arabidopsis thaliana (L.) Heynh, ecotype
Wassilewskija (Brassicaceae) Protoplasma (1995) 185:7-21

Rosemary






From daemon Tue Dec 18 08:29:56 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 18 Dec 2001 08:24:38 -0600
Subject: RE: JEOL 733 Help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It was a long time ago that I worked with 733, but as I remember
the vacuum control board has two or three variable resistors
which set thresholds, including threshold for turning on high
voltage. This board is not very stable and thresholds needs to
be readjusted from time to time. I believe it could be your
problem. Consult with manual which resistor control high
voltage threshold (or are they marked on the board?).

Good luck,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: R. Ann Bliss [mailto:bliss5-at-popcorn.llnl.gov]
} Sent: Monday, December 17, 2001 7:06 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: JEOL 733 Help needed
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Happy Holidays:
}
} I have gained the above mentioned microprobe, but it doesn't work
} yet. It was disassembled in another building, brought over and I have
} reassembled it to the best of my ability. Kind of a baptism by fire.
}
} The chiller is chilling and the water is coursing around the oil
} reservoir as well as into the appropriate parts under the column. The
} oil, used to cool the objective lens, is running from inside that
} reservoir through new hoses. And the vacuum measures in the 0-5 micro
} amp range where I'm told it should be (its actually 0.3 at the gun
} and 0.5 in the column). And the penning gauge measures in the 10^-5
} range at the main evacuation manifold - that's good, too.
}
} There is a gauge (Load/Filament) on the Accelerating Voltage panel
} that should light up "when the vacuum is at the desired level"
} according to the manual. But something is not allowing that to
} happen. Consequently, the high voltage cannot be turned on. And there
} is the rub.
}
} We do not have a service contract on this beast yet. I have called
} the service people and they have given me a considerable amount of
} help to date. We just can't get any farther than this. Is there any
} help for me and my albtross?
}
} Kind regards,
} --
}
} +++++++++++++++++++++++++++++
}
} R. Ann Bliss
} Technician, Chemistry and Materials Science
} Materials Science and Technology Division
} Lawrence Livermore National Laboratory
}
} _____________________________
}
}


From daemon Tue Dec 18 08:57:10 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 18 Dec 2001 08:50:49 -0600
Subject: Tip flashing in Hitachi FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

Regarding the recent question I posted about flashing current decreasing
with each tip flash in a Hitachi S4700 FESEM, the problem appears to have
been in the gun cable contacts. The Hitachi engineer flashed the tip
several times, getting tiny increases in current each time, then checked the
contacts on the cable. The next flash produced a MARKED increase in
flashing current and the system is now running normally.

My question was aimed more at understanding at the theory behind all this,
since the scope was performing just fine, but it turns out to have been a
simple mechanical problem fixable in about five minutes.

Thanks again for all the replies.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Tue Dec 18 09:33:24 2001



From: michael shaffer :      rarewolf-at-roadrunner.nf.net
Date: Tue, 18 Dec 2001 11:56:26 -0330
Subject: SE detector gamma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When I open a SE image into Photoshop (v6), it has been my practice to
assign a generic gamma=2.2 working space to it. Better, would be to assign
whatever space it was acquired with (i.e., the actual display profile for
the SEM computer). My thinking is, since we make all adjustments with that
display, it is the gamma space I should be associating the SE image to. And
indeed, the image appears just like it did at the SEM computer.

However ... the SEM image acuisition software only allows me to fill up
the histogram, endpoint to endpoint, without any tools for what's between
(gamma). Am I somehow doing the acquisition an injustice with respect to
the detector's true gamma?? What if the SE detector's true gamma is unity
(e.g., like x-ray maps)?? Has anyone ever determined what a SE detector's
apparent gamma is?? If for the sake of the images' data integrity,
shouldn't acquisition software be aware of detector gamma, and compensate
for the monitor's gamma??

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland



From daemon Tue Dec 18 11:06:52 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Tue, 18 Dec 2001 11:00:58 -0600
Subject: Sectioning collagen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I cannot help this person. I was wondering if anyone out their could.
Please contact him directly.

-----Original Message-----
} From: Nellis, David [mailto:dnellis-at-ncifcrf.gov]
Sent: Tuesday, December 18, 2001 11:12 AM
To: 'semguy-at-semguy.com'
Cc: Giardina, Steve


Dear Listies,

I'm having a bit of trouble sectioning epidermis and dermis. The dermis has
thick collagen bundles that tear when thin sectioned for TEM. The epidermis
stays intact.

I've tried LR White for better infiltration, initial 15% resin infiltration,
extended infiltration times, increased and decreased knife angle, glass
knives, diamond knives, decreased and increased cutting speeds.

Befuddled,

Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Tue Dec 18 11:32:02 2001



From: David Henriks :      henriks-at-southbaytech.com
Date: Tue, 18 Dec 2001 08:59:58 -0800
Subject: Re: TEM Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pradyumna:

I don't have an answer to your specific question, but I do have a paper
titled "Technique for the Preparation of TEM Plan-View and Cross-Section
Samples of YBa2Cu3O7 Thin Films on MgO and SrTiO3" by Dr. Stephen Strieffer
that may be of help. If you would like a copy of the paper, please send me
your complete mailing address off-line and I will get it out to you. We
also have many other technical papers and application notes available on our
website under "Applications Support" at www.southbaytech.com.

DISCLAIMER: South Bay Technology produces equipment and supplies which are
described in the above mentioned paper and, therefore, has a vested interest
in promoting their use.

I hope this helps.

Best regards-

David

Pradyumna Prabhumirashi wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello listers:
} I am currently trying to prepare TEM samples of polycrystalline
} strontium titanate (grain size ~20 micron) by conventional dimpling/ion
} milling. However, due to porosity, I have been having problems in
} thinning down this material. One of the suggested sample prep methods
} was to thin the material up to say ~50micron and then embed some sort of
} epoxy in it, so that the epoxy will fill in the pores.
} Could someone explain to me how to do this? What type of epoxy should I
} use and how do I ensure that the epoxy has filled up the pored?
} Thanks,
} Prad
}
} Pradyumna Prabhumirashi
} Dept. of Materials Science
} Northwestern University
} Phone: (847)491-7798
} Fax : (847)491-7820

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***




From daemon Tue Dec 18 13:29:25 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 18 Dec 2001 14:20:52 -0500
Subject: SE detector gamma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You have to be careful when referring to gamma. I read a technical article about 1-1/2 years ago that discussed the use of gamma processing and the definition of gamma in different media. It is different for display monitors, film, SEM's etc. I wish that I had photographic memory so that I could recall the points -but I do remember being surprised because of the different definitions in various fields of imaging technology. I had learned about gamma from an SEM course and thought of it as the processing curves shown in the Goldstein et al. book.

I don't think that you should be using gamma processing on SEM images after you collect them. The SEM should have gamma processing on the output of the detector amplifier to the analog to digital converter. Do it electronically to capture what you need from the image. Of course, you should set up the digital image so that as much of the gray scale is used as possible. After a digital image is acquired, the middle control in Photoshop's level control, i.e. the gamma, should only be needed to be moved slightly to adjust the average gray level of the image. If your SEM doesn't have this ability to adjust gamma before the image is digitized, then you can always do a gamma correction using the LUT (Look up table. My philosophy is to use as much of the gray scale as possible when the image is made and to put the average gray level where I like it. I adjust all the display monitors to display what a properly set up image should look like.

Another thing about the SE detector is that the PMT ages. So that if you did set up or relied on some value, over time it would change.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: michael shaffer [mailto:rarewolf-at-roadrunner.nf.net]
Sent: Tuesday, December 18, 2001 10:26 AM
To: MSA listserver


When I open a SE image into Photoshop (v6), it has been my practice to
assign a generic gamma=2.2 working space to it. Better, would be to assign
whatever space it was acquired with (i.e., the actual display profile for
the SEM computer). My thinking is, since we make all adjustments with that
display, it is the gamma space I should be associating the SE image to. And
indeed, the image appears just like it did at the SEM computer.

However ... the SEM image acuisition software only allows me to fill up
the histogram, endpoint to endpoint, without any tools for what's between
(gamma). Am I somehow doing the acquisition an injustice with respect to
the detector's true gamma?? What if the SE detector's true gamma is unity
(e.g., like x-ray maps)?? Has anyone ever determined what a SE detector's
apparent gamma is?? If for the sake of the images' data integrity,
shouldn't acquisition software be aware of detector gamma, and compensate
for the monitor's gamma??

genuinely ... michael shaffer :o)
Avalon Peninsula, Newfoundland



From daemon Tue Dec 18 14:31:09 2001



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 18 Dec 2001 14:23:13 -0600
Subject: RE: SE detector gamma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do not understand purpose of replicating SEM monitor.
I think it is more useful to set PC monitor so that it will
correspond to printed image (or to averege PC's monitor, if
digital images are going to be electronically distributed).

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: michael shaffer [mailto:rarewolf-at-roadrunner.nf.net]
} Sent: Tuesday, December 18, 2001 9:26 AM
} To: MSA listserver
} Subject: SE detector gamma
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} When I open a SE image into Photoshop (v6), it has been my
} practice to
} assign a generic gamma=2.2 working space to it. Better,
} would be to assign
} whatever space it was acquired with (i.e., the actual display
} profile for
} the SEM computer). My thinking is, since we make all
} adjustments with that
} display, it is the gamma space I should be associating the SE
} image to. And
} indeed, the image appears just like it did at the SEM computer.
}
} However ... the SEM image acuisition software only allows
} me to fill up
} the histogram, endpoint to endpoint, without any tools for
} what's between
} (gamma). Am I somehow doing the acquisition an injustice
} with respect to
} the detector's true gamma?? What if the SE detector's true
} gamma is unity
} (e.g., like x-ray maps)?? Has anyone ever determined what a
} SE detector's
} apparent gamma is?? If for the sake of the images' data integrity,
} shouldn't acquisition software be aware of detector gamma,
} and compensate
} for the monitor's gamma??
}
} genuinely ... michael shaffer :o)
} Avalon Peninsula, Newfoundland
}
}
}
}


From daemon Tue Dec 18 14:31:45 2001



From: Tom Schamp :      cts2v-at-virginia.edu
Date: Tue, 18 Dec 2001 18:29:06 -0500
Subject: What is the best technique to determine nanoparticle lattice parameters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We want to determine the lattice parameter of nanoparticles on
amorphous carbon as a function of size in the TEM. The nanoparticles
are smaller than an extinction length, so I can't use dynamical
diffraction techniques such as CBED. We are planning on coating the
backside of the carbon film with a standard such as gold to use as a
reference for SADP analysis. Does anyone know of a better technique to
use to determine the lattice parameter of these nanoparticles in the
TEM?

Thank you in advance,

Tom Schamp

University of Virginia
Dept. of Materials Science
cts2v-at-virginia.edu



From daemon Tue Dec 18 15:06:22 2001



From: Wilson, Nick :      nwilson-at-nrcan.gc.ca
Date: Tue, 18 Dec 2001 15:59:42 -0500
Subject: Wanting to purchase a CL detector for a Philips XL30 SEM - Any a

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

We are considering purchasing a CL detector for our Philips (now FEIC) XL30
SEM. We are looking for a cost effective CL detector for diagenetic and
paragenetic studies of geological materials, typically sandstones. I have
looked over the Gatan (formerly Oxford) web site and I wondered if any users
out there have used these detectors? If so could you send recommendations or
advice regarding the possible options for CL detectors? If there are any
other companies out that also make detectors please let me know.

Thanks in advance,

Nick Wilson


Dr. Nick Wilson
Research Scientist
Geological Survey of Canada
3303-33rd St. N.W.
Calgary AB, Canada T2L 2A7
Tel: 403-292-7045
Fax: 403-292-7159
Email: nwilson-at-nrcan.gc.ca



From daemon Tue Dec 18 15:08:33 2001



From: Richard Cole :      rcole-at-wadsworth.org
Date: Tue, 18 Dec 2001 16:04:59 -0500
Subject: position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Group

Job Announcement:
Electron Microscopy (full time, permanent) Research Scientist level
Health Research Inc., Wadsworth Center, Albany, NY 12058.

We are looking for a motivated, mature and
responsible individual to join a state-of-the-art Federal funded biomedical
research laboratory. Flexible job hours in a challenging and stimulating
research environment. Will train for specialized tasks.

Minimum qualification: B.S. in biology with experience in specimen
sectioning and
electron microscopy.
Preferred qualification: B.S. in biology with experience in serial
sectioning and
electron microscopy.

Responsibilities:
1. Embedding various biological specimens for transmission electron
microscopy analyses.
2. Thin and thick (serially) sectioning of specimens.
3. Scanning and photographing sections with the EM
4. Conducting 3D analyses.




Contact:
Richard Cole
Research Scientist III
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-486-4901 Fax



From daemon Tue Dec 18 15:16:21 2001



From: Chun-Ming Li :      lichun-at-uiuc.edu
Date: Tue, 18 Dec 2001 15:06:56 -0600
Subject: Re: What is the best technique to determine nanoparticle lattice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tom,

I think you can try to determine it by means of HRTEM.

Chun-Ming Li
Dept. of Materials Science and Engineering
University of Illinois at Urbana-Champaign
1304 West Green St.
Urbana, IL 61801
Email: lichun-at-uiuc.edu


Tom Schamp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
} We want to determine the lattice parameter of nanoparticles on
} amorphous carbon as a function of size in the TEM. The nanoparticles
} are smaller than an extinction length, so I can't use dynamical
} diffraction techniques such as CBED. We are planning on coating the
} backside of the carbon film with a standard such as gold to use as a
} reference for SADP analysis. Does anyone know of a better technique to
} use to determine the lattice parameter of these nanoparticles in the
} TEM?
}
} Thank you in advance,
}
} Tom Schamp
}
} University of Virginia
} Dept. of Materials Science
} cts2v-at-virginia.edu



From daemon Tue Dec 18 15:58:24 2001



From: Seijo, Edward R. :      SeijoER-at-moffitt.usf.edu
Date: Tue, 18 Dec 2001 16:04:04 -0500
Subject: Need feedback on inverted microscopes for microinjection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a group in our institution that is putting together a setup
for microinjection. The group interested in this setup is going to be
microinjecting fluorescent labeled compounds into adherent cells. We are
currently looking at a Zeiss Axiovert 25 CFL. This is a very popular
microscope which allow for phase contrast and fluorescence. The only
drawback of this microscope and others in this price range is that the
mercury lamp is only 50 Watts. The 50W lamp is fine for routine fluorescence
but my question is whether it will be effective in picking up dimly lit
samples like the fluorescent compounds we are going to microinject?

Any thoughts?

Thanks.








From daemon Tue Dec 18 16:20:57 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 18 Dec 2001 17:13:44 -0500
Subject: request for help/service TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this query, and I cannot help her. Anyone out there who
can? Please respond offline to Dr. Marszal.

We wonder if it is possible to
have TEM images (with negative staining) taken in your facility as a
service. We need to analyze protein polymer samples. If it can be done as a
service, what fee could we expect per sample? Such an analysis was done for
us before and we know the approximate conditions of the experiment. Also, if
it can be done, how soon can we do it? If you do not provide the service,
what are the other options; can I participate in the analysis although I
have never operated an electron microscope?


Ewa Marszal, PhD
FDA/CBER
HFM-345
1401 Rockville Pike, Suite 200 N
Rockville, MD 20852-1448
phone: 301-402-4638
fax: 301-402-2780



--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Tue Dec 18 17:01:13 2001



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 18 Dec 2001 17:01:49 -0500
Subject: Re: Sectioning collagen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tim,

I'd suggest a harder resin, an epoxy type, to give support to the collagen
bundles. Try Spurrs or Embed812, formulated for a harder block. Both are
also a fairly low viscosity type resin. You may have to try a few hardness
formulas to get it right. Use the diamond knife, if its in good shape, that
is.

Good luck,

Gib

--
Gib Ahlstrand
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)625-5754 FAX, giba-at-puccini.cdl.umn.edu
http://www.cbs.umn.edu/ic/

} Dear Listies,
}
} I'm having a bit of trouble sectioning epidermis and dermis. The dermis has
} thick collagen bundles that tear when thin sectioned for TEM. The epidermis
} stays intact.
}
} I've tried LR White for better infiltration, initial 15% resin infiltration,
} extended infiltration times, increased and decreased knife angle, glass
} knives, diamond knives, decreased and increased cutting speeds.
}
} Befuddled,
}
} Tim Quinn
} University of Kansas
} Research Assistant
} Natural History Museum and Biodiversity Research Center
} Dyche Hall Room 414
} Lawrence, KS 6604-2454
} 785-864-4556
} tquinn-at-ku.edu




From daemon Tue Dec 18 17:30:23 2001



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 18 Dec 2001 18:22:35 -0500
Subject: Re: What is the best technique to determine nanoparticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might want to try HRTEM. Put your samples on amorphous Ge with gold particles. You can use the spots from an FFT of the gold particles to use as a reference standard for your magnification. Then you can use the spots (calibrated from the Au particles) in the FFT from your particles to give you the lattice spacings seen in your particles and count the fringes. Alternatively, you could use the mag from the calculated from the gold particles.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8515 (fax)



-----Original Message-----
} From: Chun-Ming Li [mailto:lichun-at-uiuc.edu]
Sent: Tuesday, December 18, 2001 4:07 PM
To: cts2v-at-virginia.edu
Cc: Microscopy-at-sparc5.microscopy.com


Hi Tom,

I think you can try to determine it by means of HRTEM.

Chun-Ming Li
Dept. of Materials Science and Engineering
University of Illinois at Urbana-Champaign
1304 West Green St.
Urbana, IL 61801
Email: lichun-at-uiuc.edu


Tom Schamp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
} We want to determine the lattice parameter of nanoparticles on
} amorphous carbon as a function of size in the TEM. The nanoparticles
} are smaller than an extinction length, so I can't use dynamical
} diffraction techniques such as CBED. We are planning on coating the
} backside of the carbon film with a standard such as gold to use as a
} reference for SADP analysis. Does anyone know of a better technique to
} use to determine the lattice parameter of these nanoparticles in the
} TEM?
}
} Thank you in advance,
}
} Tom Schamp
}
} University of Virginia
} Dept. of Materials Science
} cts2v-at-virginia.edu



From daemon Tue Dec 18 22:13:47 2001



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Tue, 18 Dec 2001 23:01:06 -0500
Subject: Workshop "Digital Image Capture and Management in Microscopy"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society
30 North Mountain Avenue
Montclair, NJ 07042


Bernard Friedman
Memorial Workshop


Digital Image Capture and Management in Microscopy
May 3, 2002

A course on Digital imaging in light microscopy which will cover the
following topics:

Optical Limitations in Light Microscopy...Photographic Imaging Strategies...
Digital Imaging Strategies...Selection of Digital Capture (Camera vs.
Scanner)...
Image Processing of Captured Images...Image File Formats...Printing
Images... Color Management Systems...Database Management
Software...Presentation Software for Oral Reports...Website
Performance...Integration of Image Data with Sample Information,
Calibration, Other Data & Reports...Acrobat and html Software for Written
Reports and Archives...Examples of Efficient, Low Cost Image Handling
Systems...
Examples of Electronic Microscopy Reports and Databases

The course instructors are Mary and John McCann of McCann Imaging.

WHEN: Friday, May 3, 2002, from 9 A.M. to 5 P.M.

WHERE: 30 North Mountain Avenue, Montclair, NJ 07042. Phone (973) 744-0043

COST: $330 for N.Y.M.S. members, $360 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

HOW: Register using the form below. Limited to the first 12 registrants.
Return form with a check to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ
07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849 e-mail
donoleary-at-att.net
PLEASE POST
----------------------------------------------------------------------------
------------------------------------------------
Digital Image Capture Registration Form
N.Y.M.S. Member_________________ ($330) Non-Member__________($360)

Name____________________________________________________________________
Address__________________________________________________________________
Phone (W)_____________________(H)_____________________Fax_________________
e-mail________________________________________



From daemon Tue Dec 18 22:35:22 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 18 Dec 2001 20:29:21 -0800
Subject: SE detector gamma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Tue, 18 Dec 2001 20:28:32 -0800
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: SE detector gamma
}
} I think that gamma should be set at the computer and its
} monitor. Adobe gamma adjust works quite well. There
} are hardware units which will do a more precise job but
} at higher cost.
}
} That said, the issue of gamma can take on many meanings
} for SE signals. The total dynamic range of a signal is one
} thing. The relationship of a peak value to the overall value
} range is another. I solve this dilemma by adjusting the
} offset of the SE amplifier while independently adjusting the
} contrast (SE detector gain).
}
} A real time histogram (Soft Imaging analySIS ADDA) shows what will
} be captured. The contrast and offset can be adjusted to maximize
} the tonal range of the input signal. I think this is what other are
} suggesting. I do agree. I try to achieve this same goal. If I cannot,
} I use Lucis or analySIS' DCE to make up the deficiencies for me.
}
} gary g.
}
}
}
} At 07:26 AM 12/18/2001, you wrote:
}
} } When I open a SE image into Photoshop (v6), it has been my practice to
} } assign a generic gamma=2.2 working space to it. Better, would be to assign
} } whatever space it was acquired with (i.e., the actual display profile for
} } the SEM computer). My thinking is, since we make all adjustments with that
} } display, it is the gamma space I should be associating the SE image to. And
} } indeed, the image appears just like it did at the SEM computer.
} }
} } However ... the SEM image acuisition software only allows me to fill up
} } the histogram, endpoint to endpoint, without any tools for what's between
} } (gamma). Am I somehow doing the acquisition an injustice with respect to
} } the detector's true gamma?? What if the SE detector's true gamma is unity
} } (e.g., like x-ray maps)?? Has anyone ever determined what a SE detector's
} } apparent gamma is?? If for the sake of the images' data integrity,
} } shouldn't acquisition software be aware of detector gamma, and compensate
} } for the monitor's gamma??
} }
} } genuinely ... michael shaffer :o)
} } Avalon Peninsula, Newfoundland



From daemon Wed Dec 19 00:03:06 2001



From: Tina Schwach :      tschwach-at-mindspring.com
Date: Wed, 19 Dec 2001 00:02:01 -0600
Subject: microchemical tests for fecal matter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jamie,
It is not clear from your request whether you want to establish that the
residue is fecal matter or something else or if you want to identify the
individual bacterial species which might be within the fecal matter. It
could simply be a matter of placing some of the material into nutrient broth
to encourage the growth of whatever was able to survive, realizing of course
that if the material is dried out, most organisms would not have survived
and therefore would not be able to grow. E. coli is always the predominant
bacterial species in fecal matter, but there are also hundreds of other
organisms (anaerobic and aerobic).

There are procedures used in the food industry to test for the presence of
fecal coliforms (not just E. coli) but they do require viable organisms.
They use enrichment procedures to coax out whatever may be present, even if
only one organism was able to survive. However, before you can establish
any procedures, it would be helpful for you to know why they want this done
and what exactly they are looking for.

Dr.Tina S. Schwach, President
Microscopy Consulting Services Inc.
651-681-0112

----- Original Message -----
} From: James Martin
To: microscopy-at-sparc5.microscopy.com
Sent: Monday, December 17, 2001 3:10 PM


I am helping a museum establish a protocol to identify residues on African
tribal beads, which is thought to be fecal matter. The sample material will
be limited to micrograms and will contain mineral and other contaminants.
Can anyone recommend microchemical or micro tests that might be used to
differentiate fecal matter?

With thanks,

Jamie



From daemon Wed Dec 19 03:22:14 2001



From: Ian MacLaren :      maclaren-at-hrem.mpi-stuttgart.mpg.de
Date: Wed, 19 Dec 2001 10:15:10 +0100
Subject: Re: What is the best technique to determine nanoparticle lattice parameters in the TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} I think you can try to determine it by means of HRTEM.
}
This is very easy to interpret but accuracy is the problem. If you could
have small particles of a known structure with a well established lattice
parameter on the same grid as your nanoparticles, then it would give you an
internal calibration of the magnification. You just can't trust that 500 kX
nominal magnification on the TEM is really 500 kX.

Alternatively, if you should be able to determing the parameters using
diffraction methods.

Using SAD, the same proviso applies. A standard on the same grid would
allow internal calibration. You could use zone axis patterns if you need to
determine the structure, or if the structure is already known, you could use
a large SA aperture and collect ring patterns. These might be more accurate
(especially where the structure is simple such as cubic) since you can
calculate the parameters from several different rings and then average them.

With a known structure, it may also be possible using convergent beam
methods, using the position of the HOLZ lines in the central transmitted
disc. The biggest problem with this is that converging a beam on your
nanoparticles may fry them, or at the very least drop lots of carbon
contamination from the vacuum on them. So, getting good diffraction
patterns is rather difficult. (Cooling the specimen and using zero loss
energy filtering can help). Then the lattice parameters are determined by
comparison with computer simulated patterns.

The last method is in principle the most accurate, but requires the most
work. If you want a quick, but not quite so accurate answer, use either of
the first two methods.

I hope this helps

Best wishes

Ian MacLaren
Max Planck Institut für Metallforschung, Seestraße 92,
70174 Stuttgart, Germany
Email: ian.maclaren-at-physics.org / maclariz-at-yahoo.co.uk
Home page: http://members.tripod.co.uk/IanMacLaren/
MPI-Stuttgart (in German): http://www.mpi-stuttgart.mpg.de/



From daemon Wed Dec 19 08:20:33 2001



From: Greg Martin :      gvm-at-aretha.jax.org
Date: Wed, 19 Dec 2001 09:15:09 -0500
Subject: Third-party service for Leica LMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey Folks --

Can anyone recommend a good outfit for servicing Leica light
microscopes in the northeast? Please reply to me directly at gvm-at-jax.org.

Thanks! Greg.

Greg Martin
Light/Confocal Microscopy
Biological Imaging Service
The Jackson Laboratory
600 Main Street
Bar Harbor ME 04609
207-288-6310



From daemon Wed Dec 19 08:50:13 2001



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 19 Dec 2001 08:34:24 -0600
Subject: RE: SE detector gamma

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think the point here is two-fold: first, the idea is to collect the
best image possible on the SEM, "best image" meaning the image best
showing the data being collected, which is not necessarily the most
aesthetic image. Second, it's very difficult to determine what the
"average gamma of PC monitors" might be -- monitors on Apples
generally have a gamma of 1.8, those on Wintel machines something
like 2.2, Linux and Unix, I have no clue. Then on top of this,
different people adjust the brightness and contrast of their
computer's monitors differently. Then there's the differences between
CRT and LCD displays. Setting a monitor's display to be the same as a
printed display is also interesting. B&W isn't too bad, but color
involves color-matching software, meters for measuring colors on the
monitor screen, RGB {-} CMYK conversions ... some people get paid lots
of money to do this.

Phil

} I do not understand purpose of replicating SEM monitor.
} I think it is more useful to set PC monitor so that it will
} correspond to printed image (or to averege PC's monitor, if
} digital images are going to be electronically distributed).
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
}
} } -----Original Message-----
} } From: michael shaffer [mailto:rarewolf-at-roadrunner.nf.net]
} } Sent: Tuesday, December 18, 2001 9:26 AM
} } To: MSA listserver
} } Subject: SE detector gamma
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } When I open a SE image into Photoshop (v6), it has been my
} } practice to
} } assign a generic gamma=2.2 working space to it. Better,
} } would be to assign
} } whatever space it was acquired with (i.e., the actual display
} } profile for
} } the SEM computer). My thinking is, since we make all
} } adjustments with that
} } display, it is the gamma space I should be associating the SE
} } image to. And
} } indeed, the image appears just like it did at the SEM computer.
} }
} } However ... the SEM image acuisition software only allows
} } me to fill up
} } the histogram, endpoint to endpoint, without any tools for
} } what's between
} } (gamma). Am I somehow doing the acquisition an injustice
} } with respect to
} } the detector's true gamma?? What if the SE detector's true
} } gamma is unity
} } (e.g., like x-ray maps)?? Has anyone ever determined what a
} } SE detector's
} } apparent gamma is?? If for the sake of the images' data integrity,
} } shouldn't acquisition software be aware of detector gamma,
} } and compensate
} } for the monitor's gamma??
} }
} } genuinely ... michael shaffer :o)
} } Avalon Peninsula, Newfoundland
} }
} }
} }
} }

--
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From daemon Wed Dec 19 09:22:48 2001



From: Mark Aindow :      m.aindow-at-uconn.edu
Date: Wed, 19 Dec 2001 10:14:49 -0500
Subject: Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



University of Connecticut
Institute of Materials Science

Postdoctoral Position

One post-doctoral position is available within the Institute of Materials
Science at the University of Connecticut starting early 2002. This
position is for one year, with possible renewal for another year. The
selected person is expected to work on characterization and synthesis of
ceramic coatings, thin films, and nanocomposites. Experience in
transmission electron microscopy, scanning electron microscopy, and x-ray
diffraction is required. Some experience in synthesis/processing of
coatings/films is highly desirable. Please send complete resume and names
(and email addresses) of 3 references to: Prof. Nitin Padture at
nitin.padture-at-uconn.edu (website: http://www.ims.uconn.edu/metal).

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Prof. Nitin P. Padture, Ph.D.
Department of Metallurgy and Materials Engineering
Institute of Materials Science, Rm. 141 (New IMS Plaza)
97 N. Eagleville Road
University of Connecticut, Storrs, CT 06269-3136
Phone: (860)486-4206; FAX: (860)486-4745
Email: Nitin.Padture-at-UConn.edu
Website: http://www.ims.uconn.edu/metal
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Dec 19 10:23:31 2001



From: tartenon-at-netscape.net
Date: Wed, 19 Dec 2001 11:15:59 -0500
Subject: RE: Need feedback on inverted microscopes for microinjection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If your fluorescence is to dimly and you are not using plan fluor objectives probably not, you can always use intensified cameras to try to pick most of the fluorescence on the sample

Regards

Alfredo
"Seijo, Edward R." {SeijoER-at-moffitt.usf.edu} wrote:

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From daemon Wed Dec 19 10:43:50 2001



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 19 Dec 2001 11:41:44 -0500
Subject: Nickel thin film production

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Have any of you used a Cressington 108 sputtering unit to produce nickel films?? Thus far no one with Cressington here in the US knows if the equipment can do it and I am awaiting England response. Anything I should be aware of?? Thanks



From daemon Wed Dec 19 11:22:00 2001



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Wed, 19 Dec 2001 11:45:45 -0500
Subject: RE: TEM coccolithophorids

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Jonathan,

Sorry to be late to the discussion. I assume you want to
section the cell itself. Are they naked cells, or covered
with coccoliths? Do you care about the cell/lith
interface? I ask because I think the real trick is
sectioning the soft tissue cleanly while also retaining the
mineralized (CaCO3) coccoliths. As I recall, it works with
standard fixations and embedding (Spurrs)...and a good
diamond knife. Of course, your diamond is at risk.

If your cells are in suspension, it's easiest to pelletize
them. As for the whole procedure, I have "seen it done"
but no real experience myself! I could get you some
references and maybe an experienced contact person.

Matt

Matthew J. Lynn
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Saturday, December 15, 2001 10:21 AM, Dee Breger
[SMTP:micro-at-ldeo.columbia.edu] wrote:
}
----------------------------------------------------------
} --------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht
} ml
}
----------------------------------------------------------
} -------------.
}
}
} Hi Jonathan,
}
} I have no experience with TEM investigation of
} coccolithophorids, but for
} SEM, the folks here spray them onto stubs - they're so
} tiny they adhere on
} their own - and then sputter coat. Also, filtering
} seawater with coccos in
} it and looking at a cutout from the filter paper (in the
} SEM, at least)
} works well for us.
}
} Good luck,
} Dee
} }
}
}
} } Season's Greetings:
} }
} } I am looking for anyone with experience looking at
} } coccolithophorids using
} } TEM.
} }
} } We need to look at sections. The guys are only about 5
um
} } in diameter. I
} } have done some thin layer embedding and individual cell
} } selection before,
} } but never on anything this small. I will be checking
} } other sources of
} } information about techniques, but thought I would ask
} } here too,
} }
} } Any hints or tips on fixing, embedding, etc. that might
} } help?
} }
} } Thanks
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
}
}
}
}
**********************************************************
} *****
} Please do not publicly post any of my correspondence
} without permission
}
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 845/365-8640
} F: 845/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)


From daemon Wed Dec 19 11:27:37 2001



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 19 Dec 2001 09:21:27 -0800 (PST)
Subject: Re: Sectioning collagen

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Howdy Tim,

We section human skin in our lab and usually find if the collagen doesn't
section it is infiltration. This can usually be traced back to water
remaining in the tissue. We use freshly opened 100% etoh in small bottles
in the final dehydration steps. Even when using LRWhite which is supposed
to be more tolerant of remaining water we have found it best to mix the
LRWhite with fresh 100% etoh in the infitration steps leading up to the
pure LRWhite.

Or the block of tissue is too big.

Bob Underwood
Dermatology Research Center
U of Washington

On Tue, 18 Dec 2001, Quinn, Tim Lee wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listies,
}
} I'm having a bit of trouble sectioning epidermis and dermis. The dermis has
} thick collagen bundles that tear when thin sectioned for TEM. The epidermis
} stays intact.
}
} I've tried LR White for better infiltration, initial 15% resin infiltration,
} extended infiltration times, increased and decreased knife angle, glass
} knives, diamond knives, decreased and increased cutting speeds.
}
} Befuddled,
}
} Tim Quinn
} University of Kansas
} Research Assistant
} Natural History Museum and Biodiversity Research Center
} Dyche Hall Room 414
} Lawrence, KS 6604-2454
} 785-864-4556
} tquinn-at-ku.edu
}
}



From daemon Wed Dec 19 12:02:38 2001



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 19 Dec 2001 09:55:39 -0800
Subject: Re: SE detector gamma (long)

Contents Retrieved from Microscopy Listserver Archives
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Still an interesting topic. My current thinking, warped or
otherwise, is similar to what I previously posted. But I'd
like to clarify a couple of points and expand on some others.

I think that Scott D. Walck's response hit on some good
points--in particular, the differences between film, CRTs
and detectors. For film, there is a near-straight line
relationship between density and log of exposure (log E).
A lot of this was discussed much earlier relative to scanners.
But anyway, the bottom part of the curve is called the toe,
the middle part is called the straight line section, and the
top part is called the shoulder (Adams, 1981) The Negative,
New York: Little, Brown and Company.

The slope of the straight line portion is a measure of the
film's contrast. The slope is the ratio of the density change
to the exposure change in the straight line region. This is
also called gamma. Thus, gamma equals change in density
divided by change in log E in the straight line region.
As Adams (1981) points out, a film with higher gamma will
have higher contrast than one with lower gamma. This is
because a same change in exposure value results in
greater density change for the higher gamma film.

The purpose of Adams' Zone system was to be able to
adjust the gamma of film to match the range of light
for particular scenes. A good example is a scene with
blue sky in the upper half of the image and dark forest
in the lower half. This could easily represent six to seven
stops of light range. The film is typically able to do a
decent job within a five stop range. To increase this range,
one can combine over exposure, under exposure, over
development and under development. The net effect of
these actions is to change the slope of the film's curve--
its gamma. The SE detector will have a non-linear characteristic just like
film does. So does the scintillator.

In fine art photography, or any other type for that matter,
if the film is not exposed (no information on the negative),
no other gyrations are going to create information that
is simply not there. Thus, I over expose and under develop.

I recently asked this list about shooting microcircuit cross
sections which have high Z contrast. The problem here is
the same as the bright and dark photographic scene.
The solution is a bit different. I actually found two.

First, mix SE and BSE signals as SE-BSE. With high
Z, the BSE image is going to be the brightest. Subtracting
some amount of BSE from SE will reduce the brightest
portion of the image. The other solution is to create a
non-linear relationship between input signal and captured
signal. I did this using the log G function with my Soft Imaging
ADDA active scan system (sorry for the plug). Using the
real time histogram, the G value and offset can be set such
that the whole 256 or 65536 bit range has information in
each bin. And the bright areas will be suppressed in
maximum value--thus not saturating the image.

As far as the computer CRT is concerned, I agree completely
that it should be calibrated and then left alone. Use Adobe
gamma calibrator to do this. Free software and takes about
five minutes to set up. There is expensive hardware to do
this but it really makes no economic sense for they type
of work we are doing with SEM/TEM, etc. I think.

My SEM has a gamma control. It does the same non-linear
alteration of input versus capture as previously discussed.
Most SEMs probably have gamma controls too. Use them.
The basic idea is to set up a log relationship between input
and output rather than a linear one. Thus, as input values
increase they are reduced in recorded value whereas
lower input value are not reduced or reduced very much.

One can still take a rather crappy SEM image and post
process it on the computer to make it look much better.
There are programs which do this quite nicely. But it
is best to get the shot right when it is created, if and
when possible.

gary g.



At 07:26 AM 12/18/2001, you wrote:

} When I open a SE image into Photoshop (v6), it has been my practice to
} assign a generic gamma=2.2 working space to it. Better, would be to assign
} whatever space it was acquired with (i.e., the actual display profile for
} the SEM computer). My thinking is, since we make all adjustments with that
} display, it is the gamma space I should be associating the SE image to. And
} indeed, the image appears just like it did at the SEM computer.
}
} However ... the SEM image acuisition software only allows me to fill up
} the histogram, endpoint to endpoint, without any tools for what's between
} (gamma). Am I somehow doing the acquisition an injustice with respect to
} the detector's true gamma?? What if the SE detector's true gamma is unity
} (e.g., like x-ray maps)?? Has anyone ever determined what a SE detector's
} apparent gamma is?? If for the sake of the images' data integrity,
} shouldn't acquisition software be aware of detector gamma, and compensate
} for the monitor's gamma??
}
} genuinely ... michael shaffer :o)
} Avalon Peninsula, Newfoundland



From daemon Wed Dec 19 13:04:36 2001



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 19 Dec 2001 13:51:10 -0500 (EST)
Subject: Re: Third-party service for Leica LMs

Contents Retrieved from Microscopy Listserver Archives
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Absolutely.

TekNet 732 905-5530.

No commercial interest, just a satisfied customer.

Sara Miller


On Wed, 19 Dec 2001, Greg Martin wrote:

} Date: Wed, 19 Dec 2001 09:15:09 -0500
} From: Greg Martin {gvm-at-aretha.jax.org}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Third-party service for Leica LMs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hey Folks --
}
} Can anyone recommend a good outfit for servicing Leica light
} microscopes in the northeast? Please reply to me directly at gvm-at-jax.org.
}
} Thanks! Greg.
}
} Greg Martin
} Light/Confocal Microscopy
} Biological Imaging Service
} The Jackson Laboratory
} 600 Main Street
} Bar Harbor ME 04609
} 207-288-6310
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265



From daemon Wed Dec 19 13:04:36 2001



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 19 Dec 2001 13:58:18 -0500
Subject: help for Dr Marszal

Contents Retrieved from Microscopy Listserver Archives
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To everyone who responded, thank you. Dr Marszal has contacted me
to let me know that she found a lab near her, in Maryland, to do the
work.
Happy Holidays and a peacefull New Year to you all.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From daemon Wed Dec 19 13:11:01 2001



From: Monson, Frederick C. :      fmonson-at-wcupa.edu
Date: Wed, 19 Dec 2001 14:03:43 -0500
Subject: RE: Need feedback on inverted microscopes for microinjection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ed,
I just got off Google where I was surfing through lunch having
searched for "microinjection confocal". Thus, I came back to email already
loaded with a response for your question, and , of course, much more than
you wanted.
You might try to contact someone at USF-physiology to get some
near-home advice. E.g., Dr. Yip: dyip-at-hsc.usf.edu. I found him by
searching USF for "confocal". Please don't think me a pompous git (as Ron
Weasely would say) for responding this way, but I can't imagine why you
would want to expend the energy to set up a system that didn't fully image
the object.
I am in possession of an Olympus FV-300 which was acquired for
between 145 and 155k$. Remember that you will be operating on specimens in
an aqueous environment and that you should look to water immersion
objectives whether you use an inverted or upright microscope. They exist
having been designed for non-coverglass immersion (upright) or coverglass
immersion (inverted). The high res water immersion from Olympus was quoted
at around 9-10k$. WOW!!! In any case, if you can find a confocal on which
there is time available, then you might be able to pay your way by supplying
the water immersion objective - something that many purchasers don't miss
until after they have made the deal. Then again, you might get really
lucky.
Finally, if you really don't need a confocal, then you must get
advice from the apps folks at Molecular Probes about the excitation of the
fluorescent probes you want to use and their 'exciting' characteristics and
how they 'quench' when lit, and how to limit their 'quenching' - when lit.
After you get that done, you should demand a chance to test the scope,
objective(s) and illumination. Oh yes, I'm not privy to the Zeiss you
mentioned, since our inverted, and quite stable, inverted scope is from
OLYMPUS, but whatever the scope, insure that the stage doesn't move or that
the micromanipulators are stage-mounted. Twenty years ago, I would not have
tried an Olympus dealer, but I would now (and my only relationship with them
is as a user).
Advisory! One spends money for a microscope, because one needs
optical and mechanical stability. Without one or the other, even the little
funds end up wasted. Nikon made a really solid inverted scope twenty years
ago - just perfect for physiologic experiments. I would bet that one could
be found on the used market. Its only deficiency, in today's environment,
would be that it wasn't infinity corrected. Tube-length corrected lenses
are still made, however, and can be acquired for less than 9-10k$. Such
optics are usually quite good.

Two really good little books:
"Confocal Laser Scanning Microscopy", Sheppard and Shotton,
Springer-Verlag, ISBN: 0 387 91514 1. ~$35.00
"Flow Cytometry - first Principles", Givan, A., Wiley-Liss, ISBN:
0-471-56095-2

Also, some bigger tomes,

Methods in Cell Biology, vol. 33 for fluorescent methods, and
Methods in Enzymology, vol. 307(under $90), and
"Electronic Light Microscopy", Shotton, D., Wiley-Liss, ISBN:
0-471-56077-4.

Hope some of this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
eMail: fmonson-at-wcupa.edu
http://darwin.wcupa.edu/casi/







} ----------
} From: Seijo, Edward R.
} Sent: Tuesday, December 18, 2001 4:04 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Need feedback on inverted microscopes for microinjection
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have a group in our institution that is putting together a setup
} for microinjection. The group interested in this setup is going to be
} microinjecting fluorescent labeled compounds into adherent cells. We are
} currently looking at a Zeiss Axiovert 25 CFL. This is a very popular
} microscope which allow for phase contrast and fluorescence. The only
} drawback of this microscope and others in this price range is that the
} mercury lamp is only 50 Watts. The 50W lamp is fine for routine
} fluorescence
} but my question is whether it will be effective in picking up dimly lit
} samples like the fluorescent compounds we are going to microinject?
}
} Any thoughts?
}
} Thanks.
}
}
}
}
}
}
}
}


From daemon Wed Dec 19 14:30:59 2001



From: Centro de =?iso-8859-1?Q?Im=E1genes?= y =?iso-8859-1?Q?Microscop=EDa?= :      cimic-at-bg.fcen.uba.ar
Date: Wed, 19 Dec 2001 17:37:48 -0300
Subject: Looking parts of TEM Philips EM 301

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott:

After a quick poll of my technical people here it seems that typical
planar magnetron sputtering units, even with a high vacuum system, will
not sputter Nickel. This is due to the magnetic properties of the Nickel
target material which interferes with the planar magnetron permanent
magnet and will not effectively make a suitable thin film. But, you can
prepare precise nickel thin films in an ion beam sputtering system such
as our IBS/e. If you would like more information on Ion Beam
Sputtering, I'd be pleased to send it to you or you can visit our
website at www.southbaytech.com and type in the
keyword "IBS/e".

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

--
David Henriks TEL: +1-949-492-2600
South Bay Technology, Inc. FAX: +1-949-492-1499
1120 Via Callejon
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

*** www.southbaytech.com ***


Original Message -----
} From: Scott Whittaker {Whittaker.scott-at-nmnh.si.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, December 19, 2001 11:41 AM


Hello people.......

I´m looking for a Objetive Stage High Resolution for a TEM Philips EM 301.....
any other spare parts are welcome.....

If any knows Santa Claus, please contact me via email.......

merry christmas.......





Gabriel Adriano Rosa
Centro de Imagenes y Microscopia
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Ciudad Universitaria, 4 piso, Pab. II, CP 1428, Buenos Aires, ARGENTINA
TE -(54-11)-4576-3349 e-mail cimic-at-bg.fcen.uba.ar
FAX (54-11)-4576-3384



From daemon Wed Dec 19 20:41:28 2001



From: Andrew Tzanavaras :      attz-at-postoffice.pacbell.net
Date: Wed, 19 Dec 2001 18:29:04 +0200
Subject: Re: Nickel thin film production

Contents Retrieved from Microscopy Listserver Archives
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Scott,

During my last job, I had no problem sputtering Ni or NiFe alloys with a
magnetron source. I used a Sputtered Films R&D System, that employs a
conical magnetron source. The magnetic field from the gun (source) magnets
will interact with the magnetic target material, but with a sufficient
source to substrate distance, high-quality, uniform Ni films can be
achieved. IBS will undoubtedly work, but the deposition rate is inherently
slow and the technique is much more expensive than magnetron sputtering.
IBS is generally used where thickness control is extremely critical, like
giant magnetoresistive multilayer applications.

Andrew Tzanavaras, M.S.
Engineering Manager
Peripheral Storage, Inc.
USA

David Henriks wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Scott:
}
} After a quick poll of my technical people here it seems that typical
} planar magnetron sputtering units, even with a high vacuum system, will
} not sputter Nickel. This is due to the magnetic properties of the Nickel
} target material which interferes with the planar magnetron permanent
} magnet and will not effectively make a suitable thin film. But, you can
} prepare precise nickel thin films in an ion beam sputtering system such
} as our IBS/e. If you would like more information on Ion Beam
} Sputtering, I'd be pleased to send it to you or you can visit our
} website at www.southbaytech.com and type in the
} keyword "IBS/e".
}
} DISCLAIMER: South Bay Technology produces equipment and supplies as
} described above and, therefore, has a vested interest in promoting their
} use.
}
} Best regards-
}
} David
}
} --
} David Henriks TEL: +1-949-492-2600
} South Bay Technology, Inc. FAX: +1-949-492-1499
} 1120 Via Callejon
} San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
}
} *** www.southbaytech.com ***
}
} Original Message -----
} } From: Scott Whittaker {Whittaker.scott-at-nmnh.si.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, December 19, 2001 11:41 AM
} Subject: Nickel thin film production
}
} }
} ------------------------------------------------------------------------
}
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
}
} }
} }
} } Have any of you used a Cressington 108 sputtering unit to produce
} nickel
} films?? Thus far no one with Cressington here in the US knows if the
} equipment can do it and I am awaiting England response. Anything I
} should be
} aware of?? Thanks
} }
} }



From daemon Wed Dec 19 20:54:43 2001



From: Pea9000-at-aol.com
Date: Wed, 19 Dec 2001 21:49:26 EST
Subject: Re: What is the best technique to determine nanoparticle lattice parameters ...

Contents Retrieved from Microscopy Listserver Archives
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Also, beware that nanoparticles below a certain size (in the realm of a few
nanometers) are prone to certain image abberations, which leads to somewhat
erroneous lattice measurements (I believe up to 10-20%). I wish I had the
reference and more concrete info, I apologize. It was a talk at the Spring
American Chemical Society Meeting by a microscopist at Monsanto. I
unfortunately threw away the program!
Anyone else familiar with such findings?

Paul E. Anderson, Ph.D.
Department of Chemistry
Northeastern University
Boston, MA

In a message dated 12/19/01 3:14:22 AM Eastern Standard Time, lichun-at-uiuc.edu
writes:

{ { cts2v-at-virginia.edu } }


From daemon Wed Dec 19 21:30:23 2001



From: Heather19fx-at-aol.com ()
Date: Thu, 20 Dec 2001 04:24:06 +0100
Subject: hey hun

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From daemon Wed Dec 19 21:38:17 2001



From: HeatherC1-at-aol.com ()
Date: Wed, 19 Dec 2001 21:27:50 -0600 (CST)
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From daemon Wed Dec 19 23:41:51 2001



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Thu, 20 Dec 2001 16:33:43 +1030
Subject: Australian 2002 EM Conference update

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all:

The term of "chemical shift" is defined as the energy peak shift on certain
condition such as oxidization status.
Compare to the pure element, its oxidized molecule would have an energy peak
shift due to chemical bonding (with O, F etc).
It is on the order of 0.5-3 eV and won't be "seen" by any current EDS
detector. This is not the case for the F intensity low,
because the chemical shift has nothing to do with the intensity.

What you have seen on the F peak lower in intensity depends on the material
property. Some F-polymers are very sensitive
for E-beam called beam damage. The electron charge from e-beam can destroy
the bounding on certain points of molecule chain and causes it broken. The
F fragment such as CF, CF2 and CF3 (F comes with C) can be released as gas
and goes away from the vacuum. After certain period of time, the F compound
will be gone and no more F can be determined on the surface of test point.
Also there will be C-rich material left on the site causing C peak growth.
No all F-polymers are sensitive for e-beam (Teflon is the stable one). I
use EDS for F-polymer lubricant analysis (used on the surface of hard drive
disks) and I can see this phenomenon clearly. There is impossible to do
quantitative analysis for this kind of material.


Zhiyu Wang, Sr. Engineer
Maxtor Corp.
Milpitas CA

----- Original Message -----
} From: "Matt Ervin" {mervin-at-arl.army.mil}
To: "Oakley, Jeff" {oakleyj-at-rayovac.com} ; {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, December 18, 2001 2:08 PM


ACEM 17
The 17th Australian Conference on Electron Microscopy
Adelaide Convention Centre, Adelaide, SA
February 4 - 8, 2002

It's not too late to book a winter escape trip - why not combine an
exciting conference and summer holiday in Adelaide, South Australia in
Feb 2002! Registrations will be accepted right up until the conference
date, and there are a range of accommodation choices suitable for all.

Encompassing the areas of Electron, Scanned Probe and Confocal
Microscopies, this important meeting will provide an opportunity to
explore the many applications of these techniques, to keep abreast of
the latest developments and to meet and mix with many of Australia's and
the world's leaders in the field.

Invited and Keynote Speakers include:
Martin Bootman; Confocal (biological), David Cockayne; Nanostructure,
Patrick Echlin; Analytical Microscopy of Biomaterials, Mark Fricker;
Confocal (biological,) David Jefferson; Emerging Techniques, Jan
Leunissen; Immunolabelling, Sergei Magonov; SPM, Ohad Medalia; Cellular
Tomography, Greg Meeker; Microbeam Analysis, Sara Miller; Virology,
Daniel Mueller; SPM - (biological), David Williams; Microanalysis,
Nestor Zaluzec; Analytical Microscopy/Telepresence, Jamie Chapman;
Reproductive Biology, Jacques Dubochet; Cryotechniques, Alex Hyatt;
Virology/Cryotechniques,
Mary, Mah-Lee Ng; Virology/Confocal

Workshops
A series of pre-conference workshops will be held to allow participants
to gain hands-on experience in the latest techniques and applications.
Many of the invited and keynote speakers will participate as presenters,
providing a wonderful opportunity for interaction with these noted
scientists. Some of the workshops are close to full - book soon!

Cryo Scanning Electron Microscopy
Patrick Echlin, John Terlet
An introduction to SEM low-temperature techniques such as specimen
preparation (freezing methods), field-emission SEM cold stage operation,
obtaining optimum imaging conditions and image interpretation.

Environmental Scanning Electron Microscopy, Techniques and Applications
Brendan Griffin, FEI representative, Matthew Phillips
The Workshop will cover all aspects of the theory and practice of
environmental scanning electron microscopy (ESEM). The use of ESEM for a
number of applications will be shown with hands-on demonstrations.

Image Analysis
Allan Jones, Arne Muller, T.K. See Tho, Craig Noble
The first day of this 2 day Workshop will comprise lectures and
practicals that cover the major aspects of image analysis. The lectures
will cover the range from basics such as image capture and digitisation
to the latest techniques and algorithms for image processing. The
practicals will be hands-on problem solving sessions. Day 2 will be an
open forum in which participants will be able to discuss analysis
strategies for images relating to their particular interests and to
discuss current technologies and future directions with vendors of image
analysis software packages.

Leica-Aurion Workshop on Cryotechniques and Immunolabelling
Ross Boadle, Jacques Dubochet, Alex Hyatt, Jan Leunissen, Ian Lamswood,
Alasdair McDowall, OhadMedalia & Graham Tranter
The Leica-Aurion Workshop will be a major event of ACEM17. This Workshop
brings together a number of experts in the field of immunolabelling and
cryotechniques. The Workshop is based on Leica Microsystems Workshops
generally available only in Europe as well as other courses presented
locally and overseas. This comprehensive Workshop will cover all aspects
of immunolabelling and cryo-preparation techniques, including staining,
thin sectioning, immuno-histology, gold labelling, immunopathological
techniques, cryo-sectioning and cryo-staining.

Light Element Analysis: Problems and Solutions
Greg Meeker, Colin MacRae
The Workshop will focus on light element analysis issues such as sample
preparation, soft x-ray spectroscopic strategies and techniques,
calculation of density of states and matrix corrections.

Quantitative Confocal Microscopy
Martin Bootman, Guy Cox, Mark Fricker, Leann Tilley, Meredith Wallwork
The Workshop will focus on quantitative confocal microscopy techniques.
Formal presentations will be followed by sessions on the confocal and
multi-photon microscopes using techniques such as live-cell imaging, 3-D
and 4-D techniques, multi-channel imaging and fluorescence recovery
after photobleaching (FRAP).

Scanning Probe Microscopy
Sergei Magonov, Daniel Mueller, John Thomas
The full day workshop consists of 3 parts:
1. An Introduction to Scanning Probe Microscopy will cover the
history/development, principles and basic techniques (contact mode,
tapping mode, phase imaging, force distance measurements).
2. Application of SPM to biological systems including sample
preparation, imaging techniques, information obtainable etc.
3. SPM studies of polymers will highlight the techniques used and
information available with a focus on studies at elevated temperatures.
A scanning probe microscope system will be used for demonstration.
Registrants are asked to indicate their interest in the 3 topics above.
The convenor may rearrange the schedule and the time allotted
accordingly.

Trace and Major Quantitative X-ray Mapping (a Hands on Approach)
Peter Lloyd, Ken Moran
The Workshop will present a practical course on aspects of x-ray
mapping. Topics will include:
instrumentation; how to get your equipment stable: how to get as many
counts as possible; x-ray maps using ED and WD; full spectrum mapping;
trace element determinations; statistical benefits.

Virology Using Electron & Light Microscopy
Sara Miller, David Howell, Alex Hyatt, Marilyn Henderson
The virology workshop will cover techniques for preparing samples for
examination, including histological, immunohistochemical, negative
staining, and thin sectioning methods for virus detection. It will
describe the pathology, cytology and ultrastructural characteristics of
infections. It will also demonstrate a "key" for morphologically
identifying viruses down to the family or genus level so that an atlas
can be easily consulted.


The Trade Exhibition will be a comprehensive and exciting display of the
latest equipment and accessories for microscopy, imaging and analysis.
We are most grateful to our sponsors and exhibitors for their very
generous support of ACEM17.

Sponsors

Platinum: FEI Company, Leica Microsystems, OED P/L (LEO),
Soft Imaging System GmbH, Thomson Scientific Instruments (Gatan)

Gold: JEOL (Australasia) P/L

Social Functions: FEI Company (Conference Dinner),
Thomson Scientific Instruments (Gatan) (Welcome Reception), Oxford
Instruments (Zoo-B-Que)

Session Sponsors: Adelaide University, South Australian Centre for
Manufacturing

Poster Competition: ProSciTech

Micrograph Competition: Unibooks

Workshop Sponsors: Adelaide University, Aurion, Bio-Rad Laboratories
P/L, Carl Zeiss P/L, Digital Instruments Inc, FEI Company, Group
Scientific P/L, Leica Microsystems P/L, Moran Scientific P/L, Olympus,
Soft Imaging System GmbH

Exhibitors
FEI Company, Leica Microsystems, OED P/L (LEO), Soft Imaging System GmbH
(Olympus), Thomson Scientific Instruments (Gatan), JEOL (Australasia)
P/L,
ASEM Inc., Bio-Rad Laboratories P/L, Carl Zeiss P/L, EMISPEC Systems
Inc, Group Scientific, Holgate Scientific P/L (SPI), IATIA Vision
Sciences, Meeco Holdings P/L, Oxford Instruments P/L, ProSciTech, SMR
Scientific P/L, Syncroscopy

As is traditional at all ACEMs, Poster and Micrograph Competitions will
be run.
Categories for the Poster Competition will be Best Biological Poster,
Best Physical Poster and Best Student Poster. Categories for the
Micrograph Competition are SEM, TEM, SPM and Confocal. Entries will be
judged on aesthetic, photographic and scientific merit. Entry details
are available on the website.

Visit the website often for regular updates!
http://www.adelaide.edu.au/CEMMSA/acem17

Enquiries to: ACEM17-at-all-occasions.com.au


From daemon Thu Dec 20 02:37:16 2001



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 20 Dec 2001 08:39:01 +0000 (GMT Standard Time)
Subject: Re: Nickel thin film production

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Scott,

We have an Ion Tech (later called Atom Tech, last known as
MECA2000 in France) magnetron sputterer that has been
modified to sputter Fe, Ni etc. We also bought an AJA
magnetron head for magnetic materials.

The Ion Tech magnetron has a ring of small magnet stacks
around the circumference and a central magnet stack of the
opposite polarity to produce the magnetic field. We
weakened the central stack (replaced rare earth magnets
with an Fe rod) to allow magnetic materials to be
sputtered. We also use thin magnetic targets (0.5 to 1mm
thick compared to 3mm of non magnetic materials).

Not being a sputtering expert I'm rusty on the exact
details but I could check it out if you want to follow it
up.

Regards,
Ron

On Wed, 19 Dec 2001 18:29:04 +0200 Andrew Tzanavaras
{attz-at-postoffice.pacbell.net} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Scott,
}
} During my last job, I had no problem sputtering Ni or NiFe alloys with a
} magnetron source. I used a Sputtered Films R&D System, that employs a
} conical magnetron source. The magnetic field from the gun (source) magnets
} will interact with the magnetic target material, but with a sufficient
} source to substrate distance, high-quality, uniform Ni films can be
} achieved. IBS will undoubtedly work, but the deposition rate is inherently
} slow and the technique is much more expensive than magnetron sputtering.
} IBS is generally used where thickness control is extremely critical, like
} giant magnetoresistive multilayer applications.
}
} Andrew Tzanavaras, M.S.
} Engineering Manager
} Peripheral Storage, Inc.
} USA
}
} David Henriks wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Scott:
} }
} } After a quick poll of my technical people here it seems that typical
} } planar magnetron sputtering units, even with a high vacuum system, will
} } not sputter Nickel. This is due to the magnetic properties of the Nickel
} } target material which interferes with the planar magnetron permanent
} } magnet and will not effectively make a suitable thin film. But, you can
} } prepare precise nickel thin films in an ion beam sputtering system such
} } as our IBS/e. If you would like more information on Ion Beam
} } Sputtering, I'd be pleased to send it to you or you can visit our
} } website at www.southbaytech.com and type in the
} } keyword "IBS/e".
} }
} } DISCLAIMER: South Bay Technology produces equipment and supplies as
} } described above and, therefore, has a vested interest in promoting their
} } use.
} }
} } Best regards-
} }
} } David
} }
} } --
} } David Henriks TEL: +1-949-492-2600
} } South Bay Technology, Inc. FAX: +1-949-492-1499
} } 1120 Via Callejon
} } San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
} }
} } *** www.southbaytech.com ***
} }
} } Original Message -----
} } } From: Scott Whittaker {Whittaker.scott-at-nmnh.si.edu}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Wednesday, December 19, 2001 11:41 AM
} } Subject: Nickel thin film production
} }
} } }
} } ------------------------------------------------------------------------
} }
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} }
} } }
} } }
} } } Have any of you used a Cressington 108 sputtering unit to produce
} } nickel
} } films?? Thus far no one with Cressington here in the US knows if the
} } equipment can do it and I am awaiting England response. Anything I
} } should be
} } aware of?? Thanks
} } }
} } }
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Dec 20 04:00:20 2001



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Thu, 20 Dec 2001 10:53:25 +0100
Subject: List of Microscopy Meetings for 2002

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I should like to inform you, that the list of microscopy meetings for
the year 2002 at so called Petr's Microscopy Resources has been
extended. You can see it at the

http://www.petr.isibrno.cz/microscopy/meetings.php#2002

Regards,

Petr Schauer
+---------------------------------------------------------------------+
| Dr. Petr Schauer tel.: (+420 5) 41514313 |
| Head of Electron Microscopy Laboratory fax : (+420 5) 41514404 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS (+420 5) 41514402 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+---------------------------------------------------------------------+


From daemon Thu Dec 20 06:59:32 2001



From: Steve Thomas :      THOMASS-at-biosrv1.bham.ac.uk
Date: Thu, 20 Dec 2001 12:51:21 -0000
Subject: receiving junk emails

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Is anybody else receiving daily junk emails from this list. These range from offers of money to
offers of sex and more!!!

The "to" box of the emails is the group email address and I've only been recieving them since I
joined this list. Is there any way to stop them other than setting a mail rule for all messages
from this group which would be silly as i would miss out on all the emails!


Thanks

Steve Thomas

*********************************
Dr Steve Thomas
School Of Biosciences
The University of Birmingham
Edgbaston
Birmingham
B15 2TT
0121 414 5573
Email: S.Thomas-at-bham.ac.uk


From daemon Thu Dec 20 08:33:09 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Thu, 20 Dec 2001 08:24:02 -0600
Subject: RE: What is the best technique to determine nanoparticle lattice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There is also an error component in lattice parameter measurements due to
tilt of the particles away from the exact Bragg condition. Think of the
situation in reciprocal space, where the reflections are in general
elongated. Depending on particle tilt, the Ewald sphere cuts the streaked
reflection somewhere along its length and the answer you get - the measured
d-spacing - depends on where the relrods are cut.

There was a nice talk by Pete Crozier et al at the M&M conference '01 (page
1078 of proceedings) which deals with this topic. In it is a reference to
an article in J. Electron Microscopy 48 (1999) p. 1015. I would imagine you
could get more details on this there.

Regards,
Wharton


} -----Original Message-----
} From: "Pea9000-at-aol.com"-at-sparc5.microscopy.com
} [SMTP:"Pea9000-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Wednesday, December 19, 2001 8:49 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: What is the best technique to determine nanoparticle
} lattice parameters ...
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Also, beware that nanoparticles below a certain size (in the realm of a
} few
} nanometers) are prone to certain image abberations, which leads to
} somewhat
} erroneous lattice measurements (I believe up to 10-20%). I wish I had the
} reference and more concrete info, I apologize. It was a talk at the Spring
}
} American Chemical Society Meeting by a microscopist at Monsanto. I
} unfortunately threw away the program!
} Anyone else familiar with such findings?
}
} Paul E. Anderson, Ph.D.
} Department of Chemistry
} Northeastern University
} Boston, MA
}
} In a message dated 12/19/01 3:14:22 AM Eastern Standard Time,
} lichun-at-uiuc.edu
} writes:
}
} { { cts2v-at-virginia.edu } }


From daemon Thu Dec 20 08:45:37 2001



From: zaluzec-at-microscopy.com
Date: Thu, 20 Dec 2001 08:35:11 -0600
Subject: Re: receiving junk emails - Filters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve

The answer is no, in general, but a few slip through. For example
2 slipped throught the fliters last night, and doing a trace I see that
they originated via 2 automated reply sites which then used some
public software list which
subsequently sent the messages to this listserver. This Server
attempts to filter most of this stuff
out but only when the culprits, phrases, keywords etc.... get added to the
filter system databses. I monitor things and when the occasional one
gets through I add the
keywords/phrases to the "database" , so the same route should, in
principle, never occur
again. No filter is perfect and some will get through.

Just to give you an order of magnitude ~ 100 junk mail messages are
rejected / week. So the one or two that slip through represent only the tip
of the iceberg.

Nestor

Your Friendly Neighborhood SysOp


From daemon Thu Dec 20 09:23:02 2001



From: Quinn, Tim Lee :      tquinn-at-ku.edu
Date: Thu, 20 Dec 2001 09:01:43 -0600
Subject: Befuddled thanks all for collagen info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listies,

A general consensus of the info received suggests ideal fixation and
embedment conditions for skin would be-

1.Use only unopened ETOH for final 100% dehydration steps. And possibly
increase amount of 100% ETOH steps.

2. Use Spurrs resin, maybe adjusted to be slightly harder than normal.
Increase infiltration times substancially.

3. Make block face smaller. Cut through epidermis first (least resistance).

I'll be working with blue monkey scrotum and blue mandrill face skin
samples. I'll let everyone know how the results turn out.

Thanks to all.
Tim Quinn
University of Kansas
Research Assistant
Natural History Museum and Biodiversity Research Center
Dyche Hall Room 414
Lawrence, KS 6604-2454
785-864-4556
tquinn-at-ku.edu


From daemon Thu Dec 20 10:46:10 2001



From: Jeff Shallenberger :      jxs124-at-psu.edu
Date: Thu, 20 Dec 2001 11:38:24 -0500
Subject: used Electroscan E-3 ESEM with heating/cooling stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Penn State University is looking too sell an Electroscan E-3 ESEM for a
nominal fee. Interested parties can contact:

Jeff Shallenberger
jxs124-at-psu.edu
814-865-0337



From daemon Thu Dec 20 11:34:12 2001



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Thu, 20 Dec 2001 11:09:38 -0600
Subject: RE: Oil backstreaming into SEM chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Listers,

I wanted to thank all who replied to my query on oil backstreaming into the
chamber of SEM's, and to provide a summary of the responses.

Owners of multiple makes/models of SEM's replied, and the impression was
that this is a very common problem.

The majority of repliers, but not all, indicated that the oil was probably
coming from the backing pump. It was indicated that this can be easily
tested by IR, which will detect if it is silicone or hydrocarbon.
Unfortunately, I cleaned the detector already so that will have to wait for
possible future occurrences.

About cleaning the detector, it was pointed out that this can be done by
removing the collimator, cleaning that with acetone/methanol, and dribbling
methanol over the head of the detector. However, beware! It was by doing
this that I somehow caused the thin window to fail. In short, this process
should be avioded if possible as it can shorten the life of a thin polymer
window, or the adhesive which seals it. If you are doing this frequently,
you should act now to get rid of the backstreaming.

Some responders indicated that DP oil can separate into two components at
advanced age (which is the case for this instrument). The lighter component
can then backstream into the chamber, i.e. it is possible that this is
occurring. This is supported by one response which claimed that the
mechanical pump had been replaced with a scroll pump and the DP left alone -
the oil problem didn't go away.

Several proposed solutions

1) Install a foreline trap and keep it maintained
2) Cut two holes in a refrigerator and insert foreline through it so that
trap is inside frig (!)
3) Eliminate oil-based system entirely and go to an oil-free turbo/scroll
system.
4) Install a system such as SEM-clean (XEI Scientific), essentially
injecting a slow stream of dry nitrogen into the foreline to produce a
constant gas flow, which will block oil from backstreaming.
5) Change the switchover from rouging to diffusion pumping to a lower
pressure. If the pressure in the chamber is above 100 millitor when the
switchover occurs this will be too much for the DP and will cause turbulent
flow in the DP leading to backstreaming. 70 millitor was recommended. It
was noted that this is a problem on the Hitachi S570.
6) Check cooling lines to cold trap to ensure it is working (we don't have
one).
7) Have the detector pumped out so that it will insulate better and the tip
won't be cold (eliminates condensation).
8) Get a better mechanical pump. (Our mechanical pump was in fact getting
rather hot - too hot to touch - when running continuously, which I gather is
a characteristic of that particular pump).
9) Get Will Bigelow's book and learn more about this problem.

So what do you do when you don't have unlimited time to fiddle or money to
spend? Do what is easiest and cheapest and hope it works.

This eliminates #2, 3, and 4. I have installed the foreline trap
(bakeable). I also found a better mechanical pump, had it serviced and
installed it, also changing all the rubber hose. By default I am doing #7
because the detector needs service. I know I should do #9. Number 5 is
very interesting and I intend to mention it to our service people to see if
it can easily be accomplished.

My advice - If you are buying a new SEM, an oil free vacuum system is a very
good idea. Right now I am going to wait and see what I get from the
improvements already made. I will let you know how it goes.

Happy Holidays to all,

Wharton


From daemon Thu Dec 20 11:59:51 2001



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Thu, 20 Dec 2001 10:06:49 -0800
Subject: Re: What is the best technique to determine nanoparticle lattice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sorry if this is repeat information, I deleted the other "EDS chemical
shift" emails.

The microcalorimeter being developed with the support of NIST represents
hardware capable of yielding chemical information from x-ray
micro-analysis. If and when this becomes a commerical product it will have
the energy resolution needed to provide "chemical shift" type information.

For those interested in the microcalorimeter project here is a websit link}
http://www.boulder.nist.gov/div814/div814/news/microcal/microcal.htm

Regarding beam damage to fluorinated polymers, it is a fact of life. Every
system has a critical dose that is a function of the area of the beam and
the current. You can reduce the damage rate by rastering over a larger
area, if that is an option. You can check for this effect by acquiring
spectra over time and plotting a ratio of the components (say F:C ratio).
You can then even assign a "damage parameter" based upon the data
exponents. A higher voltage for a given current will tend to reduce the
damage rate also, because the same current is distributed over a larger
area.


Regards,
Ed Principe
Defect & Thin Film Characterization Lab
Applied Materials
Santa Clara, Ca.



"Zhiyu Wang" {zhiyuw-at-attbi.com} on 12/19/2001 01:31:19 PM

Please respond to "Zhiyu Wang" {zhiyuw-at-attbi.com}


To: "Oakley, Jeff" {oakleyj-at-rayovac.com} , "Matt Ervin"
{mervin-at-arl.army.mil} , "'Microscopy-at-MSA.Microscopy.com'"
{Microscopy-at-sparc5.microscopy.com}
cc:


Hi, all:

The term of "chemical shift" is defined as the energy peak shift on certain
condition such as oxidization status.
Compare to the pure element, its oxidized molecule would have an energy
peak
shift due to chemical bonding (with O, F etc).
It is on the order of 0.5-3 eV and won't be "seen" by any current EDS
detector. This is not the case for the F intensity low,
because the chemical shift has nothing to do with the intensity.

What you have seen on the F peak lower in intensity depends on the material
property. Some F-polymers are very sensitive
for E-beam called beam damage. The electron charge from e-beam can destroy
the bounding on certain points of molecule chain and causes it broken. The
F fragment such as CF, CF2 and CF3 (F comes with C) can be released as gas
and goes away from the vacuum. After certain period of time, the F
compound
will be gone and no more F can be determined on the surface of test point.
Also there will be C-rich material left on the site causing C peak growth.
No all F-polymers are sensitive for e-beam (Teflon is the stable one). I
use EDS for F-polymer lubricant analysis (used on the surface of hard drive
disks) and I can see this phenomenon clearly. There is impossible to do
quantitative analysis for this kind of material.


Zhiyu Wang, Sr. Engineer
Maxtor Corp.
Milpitas CA

----- Original Message -----
} From: "Matt Ervin" {mervin-at-arl.army.mil}
To: "Oakley, Jeff" {oakleyj-at-rayovac.com} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, December 18, 2001 2:08 PM


Examples of such errors are discussed in the following:
"Deceptive "Lattice Spacings" in High-Resolution Micrographs of Metal Nanoparticles", J.-O. Malm and M.A. O'Keefe, Ultramicroscopy 68 (1997)13-23.
"Fresnel effect in high resolution TEM imaging of small particles", Velimir Radmilovic and Michael A. O’Keefe, in 53rd Ann Proc. MSA, Kansas City, Missouri (1995) 564-565.
"Application of cross-correlation technique for the analysis of Fresnel effect in small particle HREM imaging", V. Radmilovic, R. Kilaas, and M.A. O'Keefe, in Proc. European. Congress for Electron Microscopy II (1996) 50-51.
"The effect of crystal tilt on high-resolution micrographs of small metal particles", Jan-Olle Malm and Michael A. O'Keefe, SCANDEM-93 (Extended Abstracts of the 45th Annual Meeting of the Scandinavian Electron Microscopy Society), Lund, Sweden (1993) 131-132.
Please contact me with further questions.
-Michael A. O'Keefe
NCEM, LBNL

"Pea9000-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Also, beware that nanoparticles below a certain size (in the realm of a few
} nanometers) are prone to certain image abberations, which leads to somewhat
} erroneous lattice measurements (I believe up to 10-20%). I wish I had the
} reference and more concrete info, I apologize. It was a talk at the Spring
} American Chemical Society Meeting by a microscopist at Monsanto. I
} unfortunately threw away the program!
} Anyone else familiar with such findings?
}
} Paul E. Anderson, Ph.D.
} Department of Chemistry
} Northeastern University
} Boston, MA
}
} In a message dated 12/19/01 3:14:22 AM Eastern Standard Time, lichun-at-uiuc.edu
} writes:
}
} { { cts2v-at-virginia.edu } }



From daemon Thu Dec 20 12:09:43 2001



From: tartenon-at-netscape.net
Date: Thu, 20 Dec 2001 13:04:34 -0500
Subject: RE: RE: Need feedback on inverted microscopes for microinjection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually Nikon has Infinity optics and longer working distances than any other maker, TE300 or TE2000 (visit Nikon USA website) are as solid as the old TMD and these new microscopes has water immersion objectives and an upright microscope with the stand fixed. The actual focusing is done through the nosepiece the N. A. for 60X Water immersion objective is 1.4 as far as I can remember

Alfredo

"Monson, Frederick C." {fmonson-at-wcupa.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




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From daemon Thu Dec 20 14:01:12 2001



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Thu, 20 Dec 2001 13:57:57 -0800
Subject: SEM: Need ISI (Topcon?) SEM service info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:
I was asked to look at an old SEM we have and I am looking
to see if there is company support for an old ISI SEM. I guess
thay have become Topcon ? Do they support older ISI SEM's.
Let me know if you have any knowledge. Thanks in advance.
Thx,
Mike Coviello
UTA



From daemon Thu Dec 20 20:05:16 2001



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 20 Dec 2001 21:00:40 -0500
Subject: AMRAY 1610 stage/door ass'y

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear collectors of valuable stuff,
One of my customers is looking for a second stage and door ass'y for his
AMRAY 1610. His present one has gotten "hot" and he would like to have
a clean one for most of his work. Makes life simpler.

Contact me off line and I can put you in touch with him if you have
something.

Thanks and Happy Holidays,
Ken Converse
owner
Quality Images
third party SEM service
Delta, PA



From daemon Fri Dec 21 00:07:19 2001



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 20 Dec 2001 21:55:17 -0600
Subject: Adhesive tabs cracking in sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A free holiday Trivia Tip:

We had noticed some time ago that our carbon adhesive tabs seemed to
develop a series of fine
cracks (looks like drying mud) after being sputter-coated. This was not a
real problem, just a minor
aesthetic annoyance when they sometimes showed up in the background of a
micrograph. However,
when a client recently expressed concern about these cracks and wondered
if sputtering was also
damaging her samples, I did a rather unscientific series of tests to see
what was going on.

If anyone is interested, we discovered that sputtering at 20mA for 60 secs
invariably caused cracking
of the tabs, but sputtering at 5mA for 60 seconds did not. Sputtering for
20 seconds at 20mA also
avoided the cracks. To see if heating was the culprit, we put an uncoated
tab on an SEM stub into a
60 degree oven for an hour. No cracks. We also ran one through the
sputter coater cycle, but did not
actually apply a coating, to see if the turbo-level vacuum alone would do
it. No cracks.

So, if cracked tabs are keeping you awake at night, use a lower sputtering
current or a shorter time.
If you couldn't care less, well, have a Merry Christmas anyway and delete
this!

Randy

Randy Tindall


From daemon Fri Dec 21 05:57:50 2001



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Fri, 21 Dec 2001 17:22:29 +0530
Subject: Thanks for Gatan Duo Mill 600 TMP - servicing information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List members:

Many thanks for the useful information received from Drs Hunt, Boucher, Wieland and Quinn. We were able to service the vac valve of the right mill. We replaced the both 'o' rings. The bellows turned out to be good, i.e., no leak there. Also thanks to Dr Mitro for the detailed instructions. Vacuum levels are really good now.

Merry Christmas to all,

----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----






From daemon Fri Dec 21 07:18:59 2001



From: jearanai-at-nsrc.or.th ()
Date: Fri, 21 Dec 2001 07:09:23 -0600
Subject: Ask-A-Microscopist: X-ray Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jearanai-at-nsrc.or.th) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
December 21, 2001 at 02:06:36
---------------------------------------------------------------------------

Email: jearanai-at-nsrc.or.th
Name: Samornmate Jearanaikoon

Organization: National Synchrotron Research Center

Education: Graduate College

Location: City, State, Country

Question: Where can I find the graduate program for studying X-ray
microscopy (emphasis on Biological research)?

---------------------------------------------------------------------------


From daemon Fri Dec 21 07:54:21 2001



From: Verlander Jill :      VERLAJ-at-medicine.ufl.edu
Date: Fri, 21 Dec 2001 08:49:21 -0500
Subject: SEM: Need ISI (Topcon?) SEM service info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike - Topcon discontinued service support years ago. We have an ISI
(Topcon) SEM that we continue to have serviced by our former ISI service
engineer, who is independent now. His name is Chuck Humphrey and his
company's name is Scanning Solutions. He has always done an excellent job
for us. You can reach him the following ways:
telephone 407 234 0676
fax 407 277 4423
email cchumph-at-attglobal.net

Good luck with the scope.

Sincerely,

Jill

Jill Verlander Reed, D.V.M.
Associate Scientist
Director,Electron Microscopy Core Facility
UF College of Medicine
P.O. Box 100215 HSC
1600 SW Archer Rd. Room RB-167
Gainesville, FL 32610-0215

-----Original Message-----
} From: Mike Coviello [mailto:coviello-at-mae.uta.edu]
Sent: Thursday, December 20, 2001 4:58 PM
To: verlaj-at-mail-cs.med.ufl.edu


Hi All:
I was asked to look at an old SEM we have and I am looking
to see if there is company support for an old ISI SEM. I guess
thay have become Topcon ? Do they support older ISI SEM's.
Let me know if you have any knowledge. Thanks in advance.
Thx,
Mike Coviello
UTA



From daemon Fri Dec 21 07:55:25 2001



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Fri, 21 Dec 2001 08:54:55 -0500
Subject: Re: Address and info request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I guess I should clear some things up here. I am actually impressed with the rapidity and thoroughness of the response from both you and the England group, I was just simultaneously polling the Listers for their experiences, which I feel is the most rapid and effective way to get the nitty gritty tips and info you will never find in any manual or instruction sheet and save several hours of trial and error work. Happy holidays to all.


Scott Whittaker
SEM Lab Manager/Senior Dishwasher
Smithsonian Institution
National Museum of Natural History MRC104
10th ST & Constitution Ave, NW
Washington DC 20560-0104
202-357-1651


} } } "James Long" {james_long-at-tedpella.com} 12/21/01 12:17AM } } }
Greetings Scott,

Please disregard my voice mail request for your equipment info and address -
I didn't realize you had sent it to Mark already. I'm sorry you have felt
bounced around, I hope that we have properly addressed (at least initially)
your questions. I have some additional information that I will send to you
as well. Ted Pella and Cressington are both very interested in you
achieving success in your project. Please let me know what else I can do to
support you.

Sincerely,

James


James Long
Materials Science Specialist
Ted Pella, Inc.
512-657-0898
james_long-at-tedpella.com
www.tedpella.com







From daemon Fri Dec 21 10:28:42 2001



From: Verlander Jill :      VERLAJ-at-medicine.ufl.edu
Date: Fri, 21 Dec 2001 11:05:31 -0500
Subject: SEM: Need ISI (Topcon?) SEM service info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike - Topcon discontinued service support years ago. We have an ISI
(Topcon) SEM that we continue to have serviced by our former ISI service
engineer, who is independent now. His name is Chuck Humphrey and his
company's name is Scanning Solutions. He has always done an excellent job
for us. You can reach him the following ways:
telephone 407 234 0676
fax 407 277 4423
email cchumph-at-attglobal.net

Good luck with the scope.

Sincerely,

Jill

Jill Verlander Reed, D.V.M.
Associate Scientist
Director,Electron Microscopy Core Facility
UF College of Medicine
P.O. Box 100215 HSC
1600 SW Archer Rd. Room RB-167
Gainesville, FL 32610-0215

-----Original Message-----
} From: Mike Coviello [mailto:coviello-at-mae.uta.edu]
Sent: Thursday, December 20, 2001 4:58 PM
To: verlaj-at-mail-cs.med.ufl.edu


Hi All:
I was asked to look at an old SEM we have and I am looking
to see if there is company support for an old ISI SEM. I guess
thay have become Topcon ? Do they support older ISI SEM's.
Let me know if you have any knowledge. Thanks in advance.
Thx,
Mike Coviello
UTA



From daemon Fri Dec 21 14:06:31 2001



From: NPGSlithography-at-aol.com
Date: Fri, 21 Dec 2001 14:56:28 EST
Subject: "Best of MSA Listserver"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Just a thought...

As intended, detailed responses to many helpful topics are often generated by
the group. However, searching through archives for the discussions that one
vaguely remembers from several years ago is quite difficult given the
limitation of a month by month search.

My thought was that perhaps a listing of "Best Of" discussions could be made
that might be as simple as a listing of topics and the starting date of the
discussion. Interested parties could then look through this summary before
searching the monthly archives. The monthly archives would then be much more
useful and repeats of common questions would be reduced. (A similar benefit
could be achieved by providing a search capability of just the subject lines
for each year.)

Overall, this listserver is a very valuable resource, and this suggestion may
be a relatively easy way to make it even more useful.

Happy Holidays to All!

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Fri Dec 21 14:06:31 2001



From: NPGSlithography-at-aol.com
Date: Fri, 21 Dec 2001 14:56:30 EST
Subject: Re: SEM: Need ISI (Topcon?) SEM service info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mike,

} was asked to look at an old SEM we have and I am looking
} to see if there is company support for an old ISI SEM. I guess
} thay have become Topcon ? Do they support older ISI SEM's.
} Let me know if you have any knowledge. Thanks in advance.

It is my understanding that ISI became Topcon, which in the US became part of
RJ Lee, which later became Aspex Instruments ("www.aspexllc.com" or
"www.rjleeinst.com"). I have no idea if they support the ISI line.

You can find a list of third party SEM service providers at
"www.jcnabity.com/service.htm".

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com


From daemon Sun Dec 23 19:13:32 2001



From: Ben Kirkup :      bckirkup-at-post.harvard.edu
Date: Sun, 23 Dec 2001 19:56:15 -0500
Subject: Tunable Dye Laser Vendor Wanted for in eAFP excitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am a graduate student working on a bacterial ecology
project, and attempting to find a vendor for a tunable
dye laser to replace my mercury lamp in exciting the
eAFP (GFP/CFP/YFP) that my bacteria produce. I would
like to be able to move fairly rapidly between widely
different frequencies, so that I can later false color
a frame with relative ease.

I was looking at Laser Science (www.laserscience.com) and
have been having major trouble getting timely service from
them even in shipping a demo unit, replying to email/phone
calls, etc.

I would like to know if there are more established or
recommended vendors, and if anyone has experience with
a machine, can give interesting data such as price range, etc.

My email is: bckirkup-at-post.harvard.edu;
my apologies that even as an MSA member, I do not regularly
read the listserv itself, so if you would please reply
privately, thank you.

ben


From daemon Wed Dec 26 10:52:20 2001



From: Jacob Bastacky :      JBastacky-at-chori.org
Date: Wed, 26 Dec 2001 08:30:55 -0800
Subject: Part Time Bio TEM position in Northern California

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Group,

We are looking for an experienced person to do TEM including specimen
preparation, embedding, sectioning, staining, and some microscopy on
individual biomedical projects. A part time position with very
flexible hours, ideal for someone who would like to work this into
life. We are building a center for microscopy in children's diseases
at Children's Hospital Oakland and have more and more projects from
within the research institute and from collaborations with physicians
and scientists at other institutions in Northern California. We
would like one or more people to be able to carry out parts of the
individual projects, working closely together with us. Someone who
takes pride in doing superb work, who has the experience to help
choose the most appropriate techniques for each project, and who
doesn't mind travelling to our sister institutions to use particular
instruments or help with experiments. I would be happy to correspond
with anyone interested at {jbastacky-at-chori.org} . Please send a
portfolio of your micrographs.
Children's Hospital is an equal opportunity employer.
--
Jacob Bastacky, M.D.
Research Physician
Children's Hospital Oakland Research Institute
5700 Martin Luther King Jr. Way
Oakland, California 94609
Telephone: 510.450.7639
email: jbastacky-at-CHORI.org
FAX: 510.450.7910

Senior Scientist, Visiting
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
sjbastacky-at-lbl.gov


From daemon Wed Dec 26 15:30:53 2001



From: James Long :      james_long-at-tedpella.com
Date: Wed, 26 Dec 2001 15:21:43 -0600
Subject: Re: Nickel thin film production

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This post was written in response to the question by Scott Whittaker
regarding Ni sputtering in a Cressington 108 sputter coater. Disclaimer: It
contains both material on Ni sputtering in general as well as specific
information regarding the Cressington instrument line that Mr. Whittaker
brings up in his question. Since Mr. Whittaker brought it up, we felt that
it was important to respond with specific information regarding instrument
models; otherwise, a general answer might still leave some possibility of
confusion. This is not meant as an “ad”, and we hope it is not taken as
such.

First, as mentioned by others, a planar magnetron system is certainly
capable of sputtering Ni, and at a much lower cost compared to an ion beam
system. You mention that you have a Cressington 108 system. After some
offline correspondence we've determined that you have a 108autoSE system.
This is important to know, since different Cressington systems have
different Ni sputtering capabilities and other Cressington users who read
this response should be clear about the capability of their particular unit.

Two major issues in sputtering Ni concern a coater’s vacuum system and
magnet system. Regarding the vacuum system, pumping capability affects the
ratio of argon to background undesirables (oxygen, water) and affects what
happens chemically at the target. Materials that oxidize are going to do so
much more readily in a 108 series unit. Your 108autoSE as well as the
108auto use a rotary pump, while a 208HR uses both a rotary and a molecular
drag pump. In short, the 208HR is pumping the chamber more than 30 times
faster than either 108. It is more capable of handling Ni, which quickly
oxidizes.

Regarding the magnet system, the strength of the magnet combined with the
thickness of the target will determine your sputtering capability. A weaker
magnet necessitates a thinner target. Your 108auto/SE as well as a 208HR
have a much more powerful magnet than a 108auto. Any attempt to sputter Ni
in a 108auto with a thick target will fail as the magnetic field cannot
penetrate to the active surface of the target. Using thin targets (.1mm)
may work but the rates will be slow. Even though your 108auto/SE has a
strong magnet system, sputtering should also only be attempted with thin
targets to optimize your setup.

There are those who use the 108auto to produce Ni films for EDX but it is
not the optimum route. The 108auto/SE would be better with its superior
magnetic system. The best choice is the 208HR with good gas handling and
magnetic systems. The 208HR sputters Ni readily with targets up to 2mm
thick.

I have more detailed product information that I would be happy to share with
anyone offline.

Disclaimer: Ted Pella, Inc. is now the exclusive representative of the
Cressington EM products in the Americas and also sells sputter targets.

James Long
Materials Science Specialist
Ted Pella, Inc.
512-657-0898
james_long-at-tedpella.com
www.tedpella.com



From daemon Wed Dec 26 21:39:49 2001



From: cg27-at-wwa.com ()
Date: Wed, 26 Dec 2001 21:28:32 -0600
Subject: Ask-A-Microscopist: LM Reference Books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cg27-at-wwa.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday,
December 21, 2001 at 13:33:29
---------------------------------------------------------------------------

Email: cg27-at-wwa.com
Name: Paul

Organization: none

Education: Graduate College

Location: DuPage County, IL

Question: Hello,

I am an adult hobbyist with a rather old but nice
B&L microscope. Can you recommend any good
reference books that would help me identify the
various microbes, bacteria, etc. that I encounter
here in Cook/DuPage? I work within the 100x -
500x range using very little preparation; my
1000x oil-imms optic is decent but rarely gets
used due to its "high-maintenance" character.

Don't hesitate to recommend a children's book
(that's all I've been able to find!) if it's
particularly good. I'm looking for a good, quick
reference that is well-illustrated; if it also
contains theory - all the better!!

Thank you very much!!

Paul


---------------------------------------------------------------------------


From daemon Wed Dec 26 21:42:25 2001



From: david.m.heckman-at-pharmacia.com ()
Date: Wed, 26 Dec 2001 21:33:47 -0600
Subject: Ask-A-Microscopist:Oil Immersion Lens?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (david.m.heckman-at-pharmacia.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Wednesday,
December 26, 2001 at 12:55:37
---------------------------------------------------------------------------

Email: david.m.heckman-at-pharmacia.com
Name: David Heckman

Organization: Pharmacia

Education: Graduate College

Location: Kalamazoo, MI USA

Question: how do I use an "oil immersion" lense, and how do I
determine which oil to use and what refraction rating to use.

---------------------------------------------------------------------------


From daemon Thu Dec 27 14:08:24 2001



From: Grazyna M Tokarczyk :      gmtokarc-at-is.dal.ca
Date: Thu, 27 Dec 2001 15:50:11 -0400 (AST)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe



From daemon Thu Dec 27 16:13:20 2001



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Thu, 27 Dec 2001 15:54:49 -0600
Subject: Bausch & Lomb Clear Glass Plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need two Bausch & Lomb Clear Glass Plates (Cat. No. 31-26-86) to replace
missing plates in the base stands of two binocular dissecting scopes. Can
someone please tell me where I might order these (from B&L or other
supplier). I can only find internet sites to the Bausch & Lomb contact
lens business. Thank you.

Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242
319-335-1070




From daemon Fri Dec 28 10:09:35 2001



From: Stephen.R.Poe-at-aphis.usda.gov
Date: Fri, 28 Dec 2001 10:58:16 -0500
Subject: Bausch & Lomb Clear Glass Plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dean,

I think the originals are available but quite expensive - to replace
severaql I needed I went to a local glass shop and they cut them out of
1/4" plate and polished the edges - for about $8.00 each - B&L were about
$24 when you could find them. You can also make them yourself out of Lexan
or plexiglass - I have been able to do these myself out of scrap material
and they worked fine and don't break. If you need more details on making
the Lexan ones (very simple with a router - takes me about a minute each)
get back to me.

Stephen Poe
USDA, APHIS, PPQ
Riverdale, MD




Dean Abel {dean-abel-at-uiowa.edu} on 12/27/2001 04:54:49 PM

To: Microscopy Society of America {Microscopy-at-sparc5.microscopy.com}
cc:


I need two Bausch & Lomb Clear Glass Plates (Cat. No. 31-26-86) to
replace
missing plates in the base stands of two binocular dissecting scopes. Can
someone please tell me where I might order these (from B&L or other
supplier). I can only find internet sites to the Bausch & Lomb contact
lens business. Thank you.

Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242
319-335-1070









From daemon Fri Dec 28 18:02:49 2001



From: Neil L. Chavigny :      neil-at-radioranch.com
Date: Fri, 28 Dec 2001 17:51:26 -0800
Subject: TEM, SEM Balzers MED 010 FS

Contents Retrieved from Microscopy Listserver Archives
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I have one Balzers Model MED 010 vacuum deposition unit that is
surplus to my needs.

Photos available -at- http://www.radioranch.com/balzers/

My asking price is $1750.00.

I do not have the manuals for this unit.

I do have the extra head for carbon coating.

This unit is in excellent condition.

You will need your own roughing pump to complete this package.

Road shipping at actual cost will be needed.

I will provide the packing.

call directly or e-mail me at neil-at-radioranch.com for information.

Thanks for the visit.

Neil L. Chavigny

--
Neil L. Chavigny
5413 Woodview Ave.
Austin, Texas 78756
512 459 6855
512 459 9196
512 517 0407 Cell
800 357 9257


From daemon Mon Dec 31 11:44:26 2001



From: zaluzec-at-microscopy.com
Date: Mon, 31 Dec 2001 11:23:34 -0600
Subject: M&M 2002 Call For Papers is On-Line

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Seasons Greetings and Happy New Year to Everyone !

The Call for Papers for the Microscopy and Microanalysis 2002
meeting is now on-line.

All the details for the meeting: pre-conference congresses, short courses,
tutorials, symposia, awards, travel, exhibition, registration, lodging
and paper submission are now avialable available at:

http://www.msa.microscopy.com/MMHomePage.html

Join MSA, MAS, MSC/SMC and IMS in Quebec City August 4-8, 2002.

You may also download a PDF copy of the complete Call for Papers from
this WWW Site.

See you in August .

Nestor
Your Friendly Neighborhood SysOp






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